LOCUS       DASFSW010000001        87430 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.001, whole genome shotgun sequence.
ACCESSION   DASFSW010000001 DASFSW010000000
VERSION     DASFSW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 87430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87430
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.001"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(354..2138)
                     /locus_tag="U1099_000001"
     CDS             complement(354..2138)
                     /locus_tag="U1099_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866358.1"
                     /translation="MKTLRFFWFYFKRYKLSFAVIFLAIVAATYLQVKTPVFLGNAIA
                     EMGKIGQAYFMANQAGQADFQPDMADFNGVMLNLFFAYAATVVASLIYTLLFTRIVAH
                     STNRMRKGLFGKLERLTVAFFDSHKDGDILSRFTSDLDNIQNAFNQSLTQVVTNIALY
                     VGMVIMMFRQDTRLALVTIASTPVALIALVIIIRLSRKYTDKQQAAVSKLNAYMDEKI
                     SGQKAIIVQGVQEETIDGFLELNEEVRRTTFKGRLFGGILFPFMNGMSLVNTAIVIFA
                     GSSIVLNDSSLETAAALGLVVTFVQYSQQYYQPIMQVAASWAELQLAFTGAHRIQEMF
                     DEPEEVRPQNAPLFIELKEGVEIKDIDFGYLPGQKVLDKVSISAPKGKMVAVVGPTGS
                     GKTTIMNLINRFYDVNGGSVAFDGRDIREYDLDSLRDKVGIVLQESVLFSGTIADNIR
                     FGDESISQEMVETAARATHIHDFIMSLPEGYETFVTDDENIFSTGQKQLISIARTLLT
                     DPQVLILDEATSNVDTVTEAKIQKAMEAIIAGRTSFVIAHRLKTILNADEIIVLKDGK
                     VIEQGNHSQLLKLNGFYAELYHNQFVFE"
     gene            complement(2139..3845)
                     /locus_tag="U1099_000002"
     CDS             complement(2139..3845)
                     /locus_tag="U1099_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866359.1"
                     /translation="MFKEAILRYKWYALASVLLTSIVVATTLMQPSYLQDVLTAVLAN
                     DKEEIVRVGKFLLLIAGIGLLAGLTNTIMAAKISQGVSADIREKTFRKIQSFSYANVE
                     EFNAGNLVVRMTNDVNQIQNLVMMLFTILMRVPLLFVGAFIMAVRTLPELWWIIVLMV
                     IIIMGIMAVVMGLMGPRFGKFQSLMDRMNSIAKENLRGIRVVKSFVQEKNQYAKFKEV
                     SNELLDLNLFIGYGFSILQPLMMFISYMAIFVSLYLVSGMLDTNIEAVGGFTSFMSYL
                     MQIMFAIIMTSFMGMQASRAAISIKRLSEVLDTEPAMTFKDGAKEDLDGRIVFDNVSF
                     TYPHDTEPTLKNISFEIESGQMVGVVGATGAGKSTLAQLIPRLFDPQEGTVSIGGRDL
                     KDISQETLRDTVSIVLQKAILFSGTIADNLRQGAPGADLERLERAAGIAQAKEFIDRL
                     DDTYESQVEERGNNFSGGQKQRMSIARGVISDPKVLVLDDSTSALDAKSEKLVQEALN
                     HDLKGTTTVIVAQKISSVVKADKILVLDEGRLIGQGTHAELVATNDVYREIYETQKGR
                     EE"
     gene            complement(3838..4287)
                     /locus_tag="U1099_000003"
     CDS             complement(3838..4287)
                     /locus_tag="U1099_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM4866360.1"
                     /translation="MGHTIADFRNLLNQIEQISETIAKEYDVEHLAGPQGWALRFIAE
                     RSEVETFVKDIEAELRISKSVASNLVKRMEKNDFIRVLPSQFDRRYKQLVLTEKGRSK
                     ICHLKSFHEEMHHSLFRGIQKEEFDLIRQVADQLKDNIQHYKEKNHV"
     gene            4509..5525
                     /locus_tag="U1099_000004"
     CDS             4509..5525
                     /locus_tag="U1099_000004"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEM4866361.1"
                     /translation="MTKQTIAILGPGSWGTALGQVLNDNGHTVRIWGNVPEQIDEINK
                     EHTNKRYFKDVILDENIKGCKDLTEALDGVDAILFVVPTKVTRLVAKQVAQALKHKVV
                     VMHASKGLEPDSHKRLSEVLEEEIPAELRSEIVVVSGPSHAEETIVRDLTLISAASKD
                     LETASYVQNLFSNHYFRLYTNNDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     ARGLTEITRLGVAMGANPLTYSGLSGVGDLIVTGTSVHSRNWRAGDQLGRGEKLEDVE
                     RNMGMVIEGISTTKAAYELAQELDVYMPITQAIYKVIYQGAGIEDAIKEIMTGEFRHE
                     NEWH"
     gene            5571..6470
                     /gene="galU"
                     /locus_tag="U1099_000005"
     CDS             5571..6470
                     /gene="galU"
                     /locus_tag="U1099_000005"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEM4866362.1"
                     /translation="MKKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALRSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNDLLKLVDETTGIRLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDTKAVPLTKQLMNDYEKTHASTI
                     AVMPVPHEEVSAYGVIAPQGEGVNGLYSVETFVEKPKPEDAPSDLAIIGRYLLTPEIF
                     EILENQKPGAGNEIQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALQH
                     PQVKDDLKQYLIDLGKKLDTKKN"
     gene            complement(6489..7166)
                     /locus_tag="U1099_000006"
     CDS             complement(6489..7166)
                     /locus_tag="U1099_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEM4866363.1"
                     /translation="MKNFFDKRYPVTNALLAVTALVFLLIQIFRFGQTTTAYTIFEFG
                     GMYGQVVRYDPTQLWRLISPIFVHIGWEHFIFNSITLLGLGYQLEGLFGSRRFFLLYL
                     LSGIMGNLFVLFFTPDVVGAGASTSLFGLFAAMALLRKFSRSPYLQVLGQRYMVLLGL
                     NLVLGLFNPTISMAGHIGGAIGGCLVVIFLPPLVEKDLFTGKQIFFSFIGYMSLIVFL
                     LGIFYLI"
     gene            complement(7150..7692)
                     /locus_tag="U1099_000007"
     CDS             complement(7150..7692)
                     /locus_tag="U1099_000007"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEM4866364.1"
                     /translation="MDKKEIRQEVLQRLKALTSSQRARWSQQLTERLLSSDTYKCSKS
                     LGTYLSMPHEFDTSYLIEQAQKDGKQIFIPKTYSQGRMDFVEYNPDDLMKSRFGVWEP
                     GTYSQPVDKSVVKLIHVPGLAWNQAGFRVGYGGGFYDRYLADYQGETVSTLADFQVYD
                     FEPDVFDIPVKELLIFEELF"
     gene            complement(7734..8861)
                     /locus_tag="U1099_000008"
     CDS             complement(7734..8861)
                     /locus_tag="U1099_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEM4866365.1"
                     /translation="MLDLIATRRALHQIPELGMEEFKTHAFLMETIENLLEDCSFAQV
                     RTWKTGILVYLTGSAPEKTIGWRADIDGLPIVEETELGFKSLHPDRMHACGHDFHMTI
                     ALGLLEKMAEQQPRNNLLFLFQPAEENLAGGMLMYEAGAFGDWLPDEFYGLHVRPDLK
                     VGQMATNRATLFAGTCEVKIRFTGKGGHAAFPHTANDALVAASYFVTQVQSVVSRNVD
                     PIEGAVVTFGSMHAGTTNNVIAETAFLHGTIRALTQNMSLLVQKRVREVAEGIALSFG
                     VDLEIELNPSGYLPVENNPQLADELMTYFDGVDGVEMIDCPPAMTGEDFGYLLNKVPG
                     VMFWLGVDTPYPLHNPRLSPKEEVLPFAVDKLSDFLKMKAN"
     gene            complement(8871..9569)
                     /gene="dapD"
                     /locus_tag="U1099_000009"
     CDS             complement(8871..9569)
                     /gene="dapD"
                     /locus_tag="U1099_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEM4866366.1"
                     /translation="MTAQKMTAQEIIAFIGNAVKKTTVKVTFEGELAGPVPAEVTKLG
                     NVLFGDWKDVEPLLTNLTENVDYVVEQDGRNSAVPLLDKRNINARIEPGAIIRDQVTI
                     GDNAVIMMGAVINIGAEIGPGTMIDMGAILGGRATVGKNSHIGAGAVLAGVIEPASAE
                     PVRVGDNVLVGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEEALRTL"
     gene            complement(9726..10259)
                     /locus_tag="U1099_000010"
     CDS             complement(9726..10259)
                     /locus_tag="U1099_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866367.1"
                     /translation="MKKKWLLIFSALLTVTSLVACSNSKEETTSKSSSTEQTSTTKSS
                     TKESTSESSTVTLKESTVTYLADAEIDAIQTIGEYKTSLNSLMDSYVADFDGLIVQLP
                     EEAQTTLKPYREQIVTMLEQQKSALDSQFATLGDDSTVIPEEARATVLDSLKTVRDQL
                     KQTMTVVHEQAQTLLNE"
     gene            complement(10574..11302)
                     /locus_tag="U1099_000011"
     CDS             complement(10574..11302)
                     /locus_tag="U1099_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEM4866368.1"
                     /translation="MKKDFFVIGDVHGKFELLLDILKKWNEERQQLIFLGDLIDRGEN
                     SKACLELVWQLVREKDAICLTGNHERMFLAWLRDPLERYDHYRRNGGDTTINSLLGRP
                     LDAPVDGLVDANAVMEQYEKLIDFVKSCPYHLETEGYIFVHAGVDLTLPDWRKTSNHD
                     KVWLRTPFHEAENTTGKMIVFGHTPVYNLYQTKLRISQIWTSSDGKMGIDGGAVYGGV
                     LHGLVLNQQGLVRDYAVGCMESTQ"
     gene            complement(11368..12717)
                     /locus_tag="U1099_000012"
     CDS             complement(11368..12717)
                     /locus_tag="U1099_000012"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEM4866369.1"
                     /translation="MTHITFDYSKVLGQFVGAQEVEYMQPQVTLADQMLRQGTGPGSD
                     FIGWLDLPENYDKEEFARIKEAAAKIQNESEVLVVIGIGGSYLGAKAAIDFLSNAFVN
                     LQTREERKAPQILYAGNSISSSYLADLVDYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKAKGAVKVEADANGWETFVVPDDVGGRFSVLTAVGLL
                     PIAAAGADIDALMEGANAARTTFTSDKIAENQAYQYAAVRNILYRKGYVTEILANYEP
                     SLQYFSEWWKQLAGESEGKDQKGINPTSANFSTDLHSLGQFIQEGNRNIFETVVRVDK
                     PRKNILIPDMAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPQQDEF
                     TLGYTFYFFELAIAISGYLNAINPFDQPGVEAYKKNMFALLGKPGFEELGAELNARL"
     gene            complement(12799..13341)
                     /locus_tag="U1099_000013"
     CDS             complement(12799..13341)
                     /locus_tag="U1099_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866370.1"
                     /translation="MFLFAFPGMGKTTLAKKYTDVVDLEMSDIKYDNSSVRHLTKEER
                     KSTKRTLKDKGYKTIYVNKAYTLHRQGKTVLVALNFLMRMLLAMSIRGGVPFHIYIPH
                     PSLKEEYRQRYISRGNNDRFIFEVMFIWYPALIPLYLLSKILPKWITVTQSGETLEDY
                     YKRENFVRFSQKLRFIQHIS"
     gene            complement(13481..13570)
                     /gene="ffs"
                     /locus_tag="U1099_000014"
     ncRNA           complement(13481..13570)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U1099_000014"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(13613..14134)
                     /gene="tadA"
                     /locus_tag="U1099_000015"
     CDS             complement(13613..14134)
                     /gene="tadA"
                     /locus_tag="U1099_000015"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEM4866371.1"
                     /translation="MNYTHEEKEYFMTQALQEARKSLEKDEIPIGCVIVKDGQIIGRG
                     HNAREELNQAIMHAEVMAIQEANNVEGNWRLLDSTLFVTIEPCVMCSGAIGLARIPQV
                     IYGATNKKFGAAGSLYDILTDERLNHRVKVETGILEAECANIMQDFFRQRRERQKAAK
                     LIMKTQEEATSDS"
     gene            complement(14414..16384)
                     /gene="tkt"
                     /locus_tag="U1099_000016"
     CDS             complement(14414..16384)
                     /gene="tkt"
                     /locus_tag="U1099_000016"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEM4866372.1"
                     /translation="MSNLSVNAIRFLGIDAIEKSKSGHPGVVMGAAPMAYSLFTKELR
                     INPAQPNWVNRDRFILSAGHGSMLLYGLLHLSGFEEVSMDEIKNFRQWGSKTPGHPEF
                     GHTAGVDATTGPLGQGISTATGFAQAERFLAAKYNRDGFPIFDHYTYVICGDGDLMEG
                     VSAEAASYAGLQKLEKLIVLYDSNDINLDGETKDSFTEDVRARYNAYGWHTDLVTDGT
                     DVDAIFAAIEKAKAAGKPSLIEIKTVIGHGSPNKQGTNAVHGAPLGAEETEATRKALD
                     WNYAPFEIPAEVYADFKANVADRGAAAYDAWVKLVEDYKVAHPELAAEVTAILEGRDV
                     VEIKPEDFPVYENGFSQATRNSSQDALNAAAKVLPTFLGGSADLAHSNMTYIKEDGLQ
                     DSVNPLNRNIQFGVREFAMGTILNGMAAHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPIEHLAGLRAMPNLNVFRPADARETQAAWYLSLTSKS
                     TPSALVLTRQNLTVEEGTDFDKVAKGAYVVYEVAGFDTILLASGSEVNLAVKAAKELE
                     AAGTKVRVVSVPSTELFDAQDAAYKEEILPNAIRRRLAIEMGTTQSWYKYVGLDGKVL
                     GIDTFGASAPAQTVIDNYGFTVENVVKLVGEL"
     gene            complement(16596..17687)
                     /gene="ulaG"
                     /locus_tag="U1099_000017"
     CDS             complement(16596..17687)
                     /gene="ulaG"
                     /locus_tag="U1099_000017"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEM4866373.1"
                     /translation="MAKVQDITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSSRGKSTKKVKDMVWGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHVHSDHMDISTAAAIINNPKLDHVKFVGPVESGDIWEKWGVPAD
                     RIIRIAPGDSFEFKDIKVHAVESFDRTCLVTLPIEGYDENGGKLAGLPVTDELMARKA
                     VNYVFETPGGTIYHGADSHFSNYFAKHGRDFNIDVAINNYGDNPIGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPM
                     DKDRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(17797..19485)
                     /locus_tag="U1099_000018"
     CDS             complement(17797..19485)
                     /locus_tag="U1099_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEM4866374.1"
                     /translation="MLLDKSSCALLRYLIGLQEPETIMAISKGLQQSRRKIYYHLEKI
                     NDALPTTVESIESLPRLGIRLTEAQKLACRDLLDSIDSYTYVMSMPERIQLMLVYICI
                     TPERVTIERLMELTEVSRNTVLNDLNEIRTQLAQEQYKMTLYVSKSHGYSLVCHPLNK
                     IQYVHSLLYTIFLDANKGFLQVLEDKLFELVGCQLLFSEEMAQFLQGQVSQLEKKLGK
                     KINRYEIEFMLKVLPFLLLSYRNMELDDLEERAILKEFEQVRERIEYRVAQELKDALL
                     QTFDLKLDEIEISLIAILLLSYRKDSDVHVFSQDFIDLKRVVERFIHHFEIHSPFELE
                     NPDELAKHLLAHCKALLFRKTYGILSKNPMVQQIQDKYSTLFAMTRVSGKILEEEWQI
                     ELSDGDIAYLTIHLGGAIRRSGTRKAQTQIVYLICDEGVSVQRLLYKQCQHHLPDKKI
                     GAVFTTEQFKSVEDLLEVDFLITTSDGLETDFPLVQVHPILDFDDVLNMTHFAKYRSL
                     ADEKQVFAVELDKLLSGYIADGRTARELKQRLQKLITNELLTNLSSQDIETDLY"
     gene            complement(19610..20227)
                     /locus_tag="U1099_000019"
     CDS             complement(19610..20227)
                     /locus_tag="U1099_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866375.1"
                     /translation="MRKFLQDSLTETAYYLELILSAILGLALLALSTLLFADLLSSLA
                     GDIQADTFIQNFLSRAMTLAVGVEFIKMLYKQSPSTVIEVLLVAIARQLIVEHGSSSD
                     YLIGIISVAILFAVRKYLFTQFDDSNNIIMRASQKVKVANIIARVKIPSEGSETLRDF
                     VTRKLNEEEKTIAIGACVYLKNVALRIDNMHGELITRVEIIKSIY"
     gene            complement(20312..21028)
                     /locus_tag="U1099_000020"
     CDS             complement(20312..21028)
                     /locus_tag="U1099_000020"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEM4866376.1"
                     /translation="MPKDLQEMRQRVYEANIALPAHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYEKLSPENMVITDLDGNVVEGDLNPSSDLATHVALYKAWPNVHAVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTAEEVNSAYEKETGAVIIEEFERRGI
                     DPVAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVARMNRYTEQINPRVEPAPSYIMD
                     KHYLRKHGPNAYYGQKGDEH"
     gene            complement(21028..21891)
                     /locus_tag="U1099_000021"
     CDS             complement(21028..21891)
                     /locus_tag="U1099_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEM4866377.1"
                     /translation="MARPIGIYEKATPKHFTWKERLEFAKELGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGVRIPTICFSGHRRYPLGSNAPALEAKSLETMKQCIEL
                     AQDLGVRVIQLAGYDVYYEEKSPETRERFIKNLRQACDWAEQAQVMLAIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDIYSEFYLGHRSIAALHLKDTYAVTEN
                     SKGQFRDVPFGQGCVDWDNMFAVLKKTNYQGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLIEKAGLN"
     gene            complement(21897..22559)
                     /locus_tag="U1099_000022"
     CDS             complement(21897..22559)
                     /locus_tag="U1099_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEM4866378.1"
                     /translation="MKHIPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICSATIPTMAAALKA
                     IKEVRGDKGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KKLVDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAAAAREFKDEIRRI
                     WG"
     gene            complement(22570..23055)
                     /locus_tag="U1099_000023"
     CDS             complement(22570..23055)
                     /locus_tag="U1099_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM4866379.1"
                     /translation="MNLQKSFTENNSIRLGLTAETWQEAVHKAVEPLIDSGAATEEYY
                     DAIIASTEEYGPYYVLMPGMAMPHAQAGVGVLKDSFALITLTKPVVFSDGKEVSVLLT
                     LAATDPKIHTSVAIPQIIALFELENSIERLIACQTPEEVLAMVEESKTSPYLEGLDLE
                     S"
     gene            complement(23118..23396)
                     /locus_tag="U1099_000024"
     CDS             complement(23118..23396)
                     /locus_tag="U1099_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM4866380.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDFQSASCSVGEA
                     KGLAAGYDIVVASNHLISELEGRTKGHLVGLDNLMDDNEIKTKLQAVL"
     gene            complement(23455..24903)
                     /locus_tag="U1099_000025"
     CDS             complement(23455..24903)
                     /locus_tag="U1099_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM4866381.1"
                     /translation="MDFLQTPLNWFSQNILQNPAFFVGLLVLVGYALLKKKPHDVFAG
                     FIKATVGYMILNVAAGGLVTTFRPILAALNYKFQIDAAVIDPYFGLAAANGKIAEEFP
                     NFVSAATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATVSLMVLFLIPALR
                     NQFGTAAIGIICGIYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKIAPKLGK
                     KEENLDNLKLPSFLNIFHDTVVASATLMLVFFGVILFILGPEIMANPEVITSGAPYNP
                     AKQAFFMYVIQTAFTFSVYLFILMQGVRMFVAELTNAFQGISSKLLPGSFPAVDVAAS
                     YGFGSSNAVLSGFAFGLIGQLITIVLLIVFKSPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAAILSFISGVLQVALGAVCVGLLELSGGYHGNIDFEVPWLPFGFAFKYLGIAGY
                     VLVCLFLLAIPQLQFAKAKDKEAYYRGEAQAE"
     gene            complement(25251..31556)
                     /locus_tag="U1099_000026"
     CDS             complement(25251..31556)
                     /locus_tag="U1099_000026"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEM4866382.1"
                     /translation="MKKKSVQNMKRTGMLEKKQIFGIRKLNVGVASVGIAAALFLSGA
                     GQLVQAEEVVLPASTSSTTLSEQDGGAVEATTTAIEATDSMVAEATAVTETADATAPE
                     EAPIEEGSIRLHFENVDETAPESQGLWTWGGVAEPSDSNQWPTDTANFSSSQVDDYGH
                     YVDIKKSETPGTIGYLVLKNGEKITESDQKVELLVPEQNEAWISSDYSVSSYEPLKDE
                     NVLRINYTREDNNYEGWGVWTWGDTTEASDGWPTGAVDFKLGKYGAYVDIPLSNGLDS
                     KLGFLLINQNNPDLAGNKTIDLAFADRKRHSQIFLRNGDDKVYTNPYFIEEKVELDTS
                     KATPGTKNVTVEASVKAPFNYNESGLVSVTVTNPENAEIVKMEVDTSAIGGGLVPIST
                     ELNRVTIKATSSTAPGTYSLPVKVYDKDNGYYETKLDVTITERVKADGEKDWDEQVIY
                     FMMTDRFYNGDTSNDKLVEGTVTNPRGLYQGGDFKGVTAKLDYLKELGVDSIWLTPIV
                     ENIPQNVGSVKDGEYYAYHGYWASNFEKLNPHLGSLADFHELIDAAAEKGINIVVDVV
                     LNHAGYGTEETFAGMVRTKEEDKQGDDQLGSLSGLPDFKTEEAAVRNQLVAWQASWLE
                     RSTTAKGNSIYAFRVDTVKHVDDTTWQHFKNELVDRDPDFHLIGETWGANYKDTKGDL
                     GIGTMDSLLDFGFKDIAKYLVNGQLKAAGKELEERSKVLTSAASLGQFLGSHDEDGFL
                     YSLGGAEKEGNLDKLKLAASLLITAKGQPVIYYGEEVGQSGQNNWPVYDNRYDFDWSK
                     VETSDITDHYQKLLAFRNANSTLLSRGDTSTLAGNDSQGWLISKRSYQDQAAYLVFST
                     NTESKEMALEVSSKDVVVTDAYTGKSYQAIEKGGKWVVQVELPTIGQGGTMLLQTEAG
                     DIVNASVQGATEEPIEAGYFRVHFKTLPSDNLSSLGLWTWDDVEKPSSDVGAWPTGAT
                     NFSTAKQDDYGYYLDIKMKDETASKISLLINNTSGDNITGDKTIERISTKMNEAWFDE
                     NYQLSLYQPLKEGCIRINYFRTDGNYDQKGLWIWGDVTDLTLGDWPNGIDFENQGKYG
                     AYIDVKLTDLPSSIGFLLLDESKSGDDVKIQQKDYSFKDLKNQTQIFLKDDDATIYTN
                     PYFVNNVRVTGVSHVSLTALEAAFTTLEGADKDSILEKLSVTDKNGQTVAVTDLVLDL
                     TSNKVRVLGDFNQENAGYTLKYGNDSFTTTMSWQLKDELYAYDGELGARVRQAGSVVD
                     MTLWSPSADSVAVVLYDKDDQSKVVGKLAMTKGDKGQWDLELNSQSGLGIADYRGYYY
                     HYEITRGGQSVLVLDPYAKSLAEWNSDLADTDPSYRIAKAAIVDPAEVGPSDLNYATI
                     PNFNQREDAIIYEAHVRDFTSDPAISDELTAQFGTFAAFAERLSYLKDLGVTHIQLLP
                     VMSYYFVNELKNAERMSEYASSNSNYNWGYDPQSYFAFTGMYSTDPTDPMKRIEEFKN
                     LVNEIHKQGMGVILDVVYNHTSKTFLFEDLEPNYYHFMEADGTAKSSFGGGRLGTTHY
                     MSRRVLVDSIKYLVDEFKVDGFRFDMMGDHDAEAIELAFTEAQKLNPNIIMLGEGWRT
                     FTGDANQPVQPADQDWMSSTDTVAVFSDEIRNTLKSGYPNEGQPAFITGGAKSVESVF
                     NNIKAQPGNFLADDPGDVIQYIAAHDNLTLFDIIAQSIKKDPSIAENYTEIHQRQRLG
                     NLLVLTAQGTPFIHSGQEYGRTKQFRHPDYKEPVSEDKVPNKAHLLTNADGTPFDYPY
                     YIHDSYDSSDAVNKFDWTKATDEALYPENTRTQAFTKGLIALRKSTDAFRLGTKEEVE
                     QKVSLISIPGQNGIAKNDVVIAYQTIASNGDRYAVFVNADSKERSFVLSDIYKELLKG
                     HVLVDGKRAGIAALTDLVGVTLTDDAVVLAPLTATVIRLPYITTAVPDTAPTAEEKPS
                     LDFTTKERTEEKILPIVEEIRYDASLSTDQSYVLQEGKTGKKVLVYQDVLIDGKVVAT
                     NLLSETVVDSETRIVVKGSMEAKKEAEKPSLSTTAQASGQATSGASKASLPITGDRQS
                     DLALLGLGLAGLGLTVAAQGRTKKSEE"
     gene            complement(31737..32678)
                     /locus_tag="U1099_000027"
     CDS             complement(31737..32678)
                     /locus_tag="U1099_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM4866383.1"
                     /translation="MTTLADVARLANVSKMTVSRVINHPEQVTQELRTLVTAAMEELN
                     YKPNVAAKALAQQRTLVVQAVILEKMDVVEPYYIDLLAGIADELNHRNYTLQLVTNPS
                     QITDQCDGYIVTGARKNDYPWLKGLRKPLILFGENQEGLPFVDSDNRLATRSATQFAL
                     SKGYERIVFVGIDLPEAFEKGREEGYKDAMQGRETHLYRLENRSRVAEEFVKNLDYLP
                     ENTCFICASDRLALGVTRGLQALGKSIPNDVGVIGFDGFFLDRMSRPALTTMKQPIRQ
                     MGALSVKHLMTILDGKPLARQGHYCQATLIERETTPQ"
     gene            complement(33043..33789)
                     /locus_tag="U1099_000028"
     CDS             complement(33043..33789)
                     /locus_tag="U1099_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM4866384.1"
                     /translation="MIQASGVKLTFGGQQVLKGIDVEVNPGEVVVILGPSGSGKTTLL
                     RSLNFLEKADAGHLVFGEDSYDLAQMTKKEIAGIRKRTAFVFQNYNLFANKTALQNVT
                     EGLIVARKVPKQEAERIGKAALDKVGLSGKYGFYPSQLSGGQQQRVGIARAIATNPEV
                     IYFDEPTSALDPELIGEVLAVMMQLAKEGMTMVVVTHEMGFARAVADRVIFMDGGRII
                     EEGRPEDIFDRPQEERTKQFLARIQKVDVE"
     gene            complement(33799..34458)
                     /locus_tag="U1099_000029"
     CDS             complement(33799..34458)
                     /locus_tag="U1099_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM4866385.1"
                     /translation="MTERVWQLLLDSFSQILVPGLLVTIPLTILSFTFGLLIAIGTAL
                     VQIAQIPILKQVARFYIWVVRGTPLLVQLYVIFFGLPSLGIVLDAFPSAVLVFSVNTG
                     AYAAETIRASIESVPKGQLEAGYSVGMSFAQTMRRIILPQAFRVAFPPLSNTLIGLVK
                     DTSLAANITVLEMFMATQQIAARTYEPFALYCEVALVYLLFSTILTKLQAYGEKKLAV
                     Y"
     gene            complement(34471..35274)
                     /locus_tag="U1099_000030"
     CDS             complement(34471..35274)
                     /locus_tag="U1099_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM4866386.1"
                     /translation="MKKIAMFTSLLFAGIVLAACNSSAGISNSTDQTLLAQIKEEGVI
                     QIGTEGAYAPYSYHDESGKLVGYDVEVAEAVAEKLGVKVEFVETEWDSMIAGLDAARF
                     DTIANQVGITDERKAKYDFSTPYTYIYGALVTQKDNTDITSFADLSGKKSANSLTSNW
                     ADLARENGAEVVGVDGFSQAVELLNTGRVDATINDNLVYLDYVKQHADAPIKVVTLTD
                     DVSTTAFPVAKGNEDLAKEIDAALAELASEGKLVEISNKYFGEDVSKAK"
     gene            complement(35426..36034)
                     /locus_tag="U1099_000031"
     CDS             complement(35426..36034)
                     /locus_tag="U1099_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEM4866387.1"
                     /translation="MSYQMIKKQFKLGLFTALIGNLLWLGMVNVSADDTVISDSPTVS
                     ALTKKVEAAKIAAKAAAEKIVAEKAAAAAKAALATNMVSEVAVEYTANTYPAGQCTWG
                     AKEMAPWVGNYWGNGGDWAASAAALGYEVGTTPKVGAIAVWTDGGYGHVAYVTDVAAN
                     GHIQVKESNYGGVYYPSNVRGFFDPTTTSEGTVSYIYPPAGV"
     gene            complement(36367..37743)
                     /locus_tag="U1099_000032"
     CDS             complement(36367..37743)
                     /locus_tag="U1099_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM4866388.1"
                     /translation="MSNISEKFMEISGKIGSQRHLIAIRDSFISMMPITMAGTVAVLL
                     NVFLRDIPNNMGWTGFADAMQPVIDINGYVYFGTIGIMALVFAFTFGYNLSLMYKVNP
                     LAGGLISFASFISTIPQSVTISTTLDGVDKSVVSALEGLGLSIVTTDGVSALETSQWG
                     AIALKYGGATGLFTALFIGFLATWVYASLVKRNVIIKLPDSVPPAVNKAFAAIIPGTA
                     AIYASSIVAYLAFTLTGQSLGDLVSTYIQLPLLGLSQGLGSVILLTFLVQLFWFFGLH
                     GHNVLAPIMDGVYMVALNENAAAYTATHSTANLPWLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEYKMVAKLSAPMGVFNINEPVTFGIPMVLNPLFAIPWLIVPPICATIA
                     YLATAAGIIPPVFAPVPWITPPGLYAYLATGGNIMAALVSLFNLFVAFLIWTPFVIAA
                     NKVKAGEE"
     gene            complement(37787..38227)
                     /locus_tag="U1099_000033"
     CDS             complement(37787..38227)
                     /locus_tag="U1099_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEM4866389.1"
                     /translation="MKISKIGQVPIERLFATIRQSLKDDFQQNTGSILQDQDIIQGLT
                     YTKTFGDKGQHSVKVTVEEFTAPYCYAVCFHSNRGKEHVRYQLEDLGNDQTNIEYSYE
                     MEAGDIFVKGNQFLMEKIFSKSIKRQTNAQLEALIRCSVEPVSV"
     gene            complement(38246..38560)
                     /locus_tag="U1099_000034"
     CDS             complement(38246..38560)
                     /locus_tag="U1099_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM4866390.1"
                     /translation="MNSPEYLEAIMGLIMYGGEAKGFASQAIQAAKEGRIDEAEQLLQ
                     DAIASLNIAHKSQTQLLAKEASGESVELSLLMVHGQDHLMNALTFIDLAKEFVDLYKQ
                     IG"
     gene            complement(38562..38873)
                     /locus_tag="U1099_000035"
     CDS             complement(38562..38873)
                     /locus_tag="U1099_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM4866391.1"
                     /translation="MKHIMLVCNAGMSTSMLVTKMQKASEETGVEATIWAVPVSEADN
                     EVAQKEIDVLLLGPQVKFLLNDYKAKFEPTIKVDAINMMDYGVMNGAKVLEAALKMME
                     G"
     gene            complement(38886..40880)
                     /locus_tag="U1099_000036"
     CDS             complement(38886..40880)
                     /locus_tag="U1099_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM4866392.1"
                     /translation="MLSKKEVRLLEALLAKQETFVPSIELAKVLSVSDRTVRTYIHQL
                     TSSLENSGAHILSKQGSGYCLQIERPVEFEMFWQEQLLLKKRLTDLTQLEEAGDRQRY
                     ILNKLLFEDPVQSPECLQRELFIGKTTLHSILADIRKLFIPYQLELLVTRKGIELKGD
                     EPAIRHFIMDYFFDEGNVQSVYGVVETNLLPDIRFTDLMLIVLDECRDAKLRLSDFVM
                     QNLVLHIALLLQRLKIGSGLDRFPIPERIQSSQEYLVAQRIIERIEEMFQVTIPSEEA
                     NYIALHLKVKLTGNPNQDGEVEEIFRQNVENSLLHLSHLTGLELSKDQQLFKGVLAHL
                     MPLRIRLENNIQLGNPLTAEIKDQYFEAFELTKTAFAAIPELMGYSISDDEWAYLTLH
                     VMAAIERYQGFKKTRVLVVCATGIGSALMLKNRLEKEFSSSLEILDTVSYYEVTEERL
                     KDVDLIISSISLSNLIFMTPVINVSVFLSEKDIEAIRSHLQQTTPVIPDQGESVLMEV
                     GQAKQIAEAVFAPHRIIYLEEDCDREVALKQLVTTLNESIEPDFVNRFIEQVNLRESY
                     SPVVFGDWLAFPHPALPMSYSEQIVVGIYKNAVDWGHGKQVQFVFLLSPSKGRNAFLK
                     YISPCLVDFVQDRVLQERLLHHPNYKELIDIFIPLIQRKG"
     gene            complement(40972..42078)
                     /locus_tag="U1099_000037"
     CDS             complement(40972..42078)
                     /locus_tag="U1099_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM4866393.1"
                     /translation="MVELGFSIYPENYSLEESQAYIKLLHQYGARRLFMSLLQLENGD
                     RTVFELYQKLVDYANQLGMTVIADISPHFIAAQGWDGALMERAAELGLAGIRLDEALP
                     LEEIIEMTHNPHNIKVELNLSTDRTLLSNLLASVANRDNIIACHNFYPHAYTGLSQEH
                     FLEMSSFYYQSGIRTAAFVSAQTASEGPWPLSEGLPTLEDHRFWLLPLQIAWMKATGF
                     IDSILISNQFISEEELQSIQTVLNEDTISLMVDIEEGLTPVEREIVAFDHVYRGDISA
                     YVLRSTMPRVVYKDASVPARSDQAIPVGRGDILIDNDLYGRYKGELQIALKEFTISPK
                     VNKVGRISPDYLPLLRFIKPWQEFKLVIASEHFH"
     gene            complement(42088..43488)
                     /locus_tag="U1099_000038"
     CDS             complement(42088..43488)
                     /locus_tag="U1099_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM4866394.1"
                     /translation="MTKDKVFYQFPTNFLWGSSTSGPQSEGIEPGDGKGQNNWDYWYS
                     IEPERFHNKIGPSQTSTFYKHYEKDIDLLVETGHTVFRTSIQWSRLIPTGTGEINQEA
                     VSFYRSVFSLVRAKGIQLFVNLYHFDLPYCLQEKGGWENKEIVRAYEAYARTCFELFD
                     DLVDRWITFNEPIVPVECGYLGNYHYPCKVDAKAAVAVAYHTQLASSLAVKACHEVNP
                     HKKIAIVLNLTPAYPRSEQAEDIKAARIAELFQTKSFLDPSVLGEYPDELVELIDAYG
                     LMPEVSDEELAIIKENRVDFLGVNYYQPLRVQAPTNSWKEGEPLTPEHFFAPYDMPGK
                     KMNPHRGWEIYEKGIYDIAINIKENYHNIEWFVTENGMGVEGEGAFKDNGLIQDDYRI
                     EFIEDHLIELHRAIQEGANCKGYMLWTFIDCWSWLNAYKNRYGLVELDLDTQERIVKK
                     SGKWFKKLSDANGFHR"
     gene            complement(43485..44846)
                     /locus_tag="U1099_000039"
     CDS             complement(43485..44846)
                     /locus_tag="U1099_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM4866395.1"
                     /translation="MLKTLKLVTIGGGSSYTPELVEGMILRAHQLPIREWWFVDVEQG
                     AKKQEIVVELARRMVAKAGLDWKIESTLDRREALIEADFVTSQFRVGQLPARYLDETI
                     PLSYGMLGQETNGAGGIFKAFRTIEAYKGIIKDMKDLCPNAWLINFTNPAGIVTEAIA
                     NELHWKKVIGLCNIPIGQKKLAAQILGQSEGDLSTRHIGLNHFHFHEVWDKYGENRTQ
                     VILETLYGDLREETPEAVKNITNLEFPIQLLRTIGMLPCDYHRYYFLEQEMLEDALEQ
                     YRQGNVRAKVVQEVETELFELYKDPQLKDKPKQLELRGGAYYSDVACQIICAIHNDSR
                     EELTVSTVNRGVIPYLPENCVVEVTSIISAQGATPLACPPTPPIVTGYLQLMKQMELV
                     TVQAAMTGDYSLALQAFTLNPLISNGPQAEVLLQDMLLAHENYLPQFAPAIQHIKERR
                     NNQ"
     gene            complement(45115..47616)
                     /locus_tag="U1099_000040"
     CDS             complement(45115..47616)
                     /locus_tag="U1099_000040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEM4866396.1"
                     /translation="MSFYNHKEIEPKWQGFWAKNHTFKTGTDAAKPNFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPADFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKIKDTDKDFTVFTTRPDTLFGATYAVLAPEHDLVDSITSPEQ
                     TEAVAEYKRQASLKSDLARTDLAKDKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFGLDIIPVLEGGNVEEAPYTEDGAHINSDFLDGLNKEEA
                     IAKMVAWLEENGVGQEKISYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPENELPLV
                     LPKTSDIKPSGTGESPLANLTDWLEVVREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NDEKLADEDLLKAWLPVDIYIGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFNIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQFGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLLTTKELAAENSGALD
                     KVYNETVKTVTEHIEDLKFNTAIAQLMIFVNAANKEDKLYVEYAKGFVQLIAPFAPHL
                     AEELWQGLTNTGQSISYVAWPTYDESKLVESEVEIVVQIKGKVKARLTVAKDLAPAEL
                     EKVALADEKVQAEIAGQTVVKVITVPNKLVNIVTK"
     gene            complement(47719..49611)
                     /locus_tag="U1099_000041"
     CDS             complement(47719..49611)
                     /locus_tag="U1099_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEM4866397.1"
                     /translation="MTRLQDDFYEYVNGEWAKTAVIPDDKPRTGGFSDLADEIEKLMI
                     DTTNAWLAGEDVPEDSVLQNFVAFHKQVADYETRDRLGAEPAQALIAEYKALNSFEEF
                     TSKLAEYELAGKPNLMPFGVAPDFMDATTNVLWADSLGIILPDTTYYEEGHEKGAELL
                     KIWRESQEALLPKFGFSDEEIKDLLDKRLELDATIAKYVLSNEEGSEYAKLYHPYEWA
                     DFTALAPELPLDDFFTAILGQTPDKIIVPEERFWQAAKDIYSADNWELLKATLILKAA
                     GAYTAFLSDEIRILAGAYSRALSGTPQAQNQEKAAYNLAQGYFNQALGLWYAGEKFSP
                     EAKADVEAKVAKMIEVYKSRLETADWLAQETRDKAIVKLNVIKPYIGYPEALPERYYK
                     KIVDPSKSLVENAIELNKIDIAHGWSKWNKPVDIKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDSNGASFDEHGSLNNWWTEEDYAA
                     FEARTQQVIDQFEGQDSYGAKINGKLTVSENIADLGGIAAALEAAKSEDDFSAEEFFT
                     NFARIWRMKARPEFMQMLASVDVHAPGHLRTNIQLPNFDEFHETFGVQEGDGMWRAKE
                     DRVIIW"
     gene            complement(49667..50506)
                     /locus_tag="U1099_000042"
     CDS             complement(49667..50506)
                     /locus_tag="U1099_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM4866398.1"
                     /translation="MFEVLLILMVIASSCGLLGSILVVKNQSMLADALSHSVLLGIVL
                     GFFISHSLDSPLLIVGASLFGLLSVLAIDRLHSRKIAHDAATGLVFSFFFAVAVLLIS
                     LFARNVHLDVDLVLQGEVLFAPLHRMDVLAWSLPVSLVKSSLAWLVIVLFFAWAYHRL
                     QVYLFDSNHARLSGLRTRILEMVILILVSLTTVLAFEAIGSMTVIVFLVAPSMAALRW
                     VKSFWQLLLLGQGIAILTVVLGFLVANQLDLTMSGTCAVVSLLVVCSSIILKNTWSRS
                     SDK"
     gene            complement(50499..51359)
                     /locus_tag="U1099_000043"
     CDS             complement(50499..51359)
                     /locus_tag="U1099_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM4866399.1"
                     /translation="MFEVFKEYSFWTVALGTVSLAVAASTIGSISVLTKQSLLGDALG
                     HASYPGVIVSFMIFQSRHPLYLLLGAVLSGYLSYVLVHWLRRKGGHSLVNALSLVSAS
                     FFGLGMVLKNAIQGNEAFAGSSQAGLQTYLFGQAAFIQLDDVILIGIISLLALALFTF
                     FYQDYKLYLFDQTFARVIGVRVKYLQQLTMFLMICLIAVGLKLVGAILMSSFLIAPAV
                     FGLMLGKSYHKSLLLAGIVAIGSAFVGTWISSSVSGLSTGPTIIVCLTGLTLSAFVYV
                     TYVRKENGRV"
     gene            complement(51352..52122)
                     /locus_tag="U1099_000044"
     CDS             complement(51352..52122)
                     /locus_tag="U1099_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM4866400.1"
                     /translation="MSAIIELKDVNLAYTASQTMALEDVNLVIPRGSRTAIVGPNGAG
                     KSSLFKVILGLEKPDTGQVRLLGQEAGLERLIAQKVAYIPQSSQVNWQFPATVFEIVL
                     MGRFAHSKGMFRRPTKADRQIVEQALERLKIADLRHRQIDQLSGGQRQRVFLARALAQ
                     EAELYLMDEPLAGIDQATEIMIMDMLKEFQCEGKTSIVIHHDLTTLDAYFDHLVWLHK
                     HVIDSGPMDETLTSENYQATYGIGNGLFLGNAKGGSHV"
     gene            52109..52435
                     /locus_tag="U1099_000045"
     CDS             52109..52435
                     /locus_tag="U1099_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866401.1"
                     /translation="MIADIFPPIFKNDMPHSYSMKIKTRPTYQAEKVDKIKIPYQQIA
                     GKPYTSLISTHQYMNLIFAEHQCFLQSYVYKSVKNGPKTERTRPRPSPFYYSFEIPIH
                     AQTTDI"
     gene            complement(52676..53599)
                     /locus_tag="U1099_000046"
     CDS             complement(52676..53599)
                     /locus_tag="U1099_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEM4866402.1"
                     /translation="MKKILFSFALLLSLIGLGACRPSQTTEGSSKPRVAVTTSFLNDM
                     VYQLAGDEVERDLLIPAGEDPHLYVAKSSDLSKLQKADLVLYHGLHFEGKMVEALEKT
                     GVAVSKNFNAKDLNTMDEDGEEIVDPHFWFSIPLYKSAVAVASEELQKLLPAKAEMIQ
                     KNAEKYQAQLDDLHAWVEKELSVIPKESRYLVTPHDAFNYFAASYDFTLYAPQGVSTD
                     SEVANSDMIETVNLIIDHNIKAIFTESTTNPERMKKLQEAVKAKGGQVEVVTGEGKEL
                     FSDSLAPEGEEGDTFIDMYKHNVKLMVKYLK"
     gene            53725..54384
                     /locus_tag="U1099_000047"
     CDS             53725..54384
                     /locus_tag="U1099_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEM4866403.1"
                     /translation="MTPNKEDYLKCIYELGQLDQKITNKLIAEKMAFSAPAVSEMLKK
                     MVAEELISKDAKAGYLLSQTALEMVASLYRKHRLIEVFLVEQLGYSPEEVHEEAEILE
                     HTVSDHFINRLDLLLEQPQTCPHGGSIPQAGQPLIERYQTRLSQLTETGNYQLVRIHD
                     FYQLLQYLEQHELAVGDLLTVTAFDQFAQTITIQYKDKELAVPTAIAQQLFIEKSNRP
                     A"
     gene            complement(54431..54694)
                     /locus_tag="U1099_000048"
     CDS             complement(54431..54694)
                     /locus_tag="U1099_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866404.1"
                     /translation="MRKFFQDLNQEYLETRSSVGEDWLEWIIQRKMTWGMRWGLVGIL
                     MFVLQPLTYNPHFLLGCFYIGFLLWLVYVCGQLFLQPDLFSVI"
     gene            complement(54800..54985)
                     /locus_tag="U1099_000049"
     CDS             complement(54800..54985)
                     /locus_tag="U1099_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866405.1"
                     /translation="MKKLLTNHFFYLTIAFLLILVIYFSGIDKRWIILASFLYFIPSQ
                     ILYRRRLRERLQEDQPE"
     gene            complement(55061..55504)
                     /locus_tag="U1099_000050"
     CDS             complement(55061..55504)
                     /locus_tag="U1099_000050"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEM4866406.1"
                     /translation="MKIVLQRVSQASVTIEGSIHGQIEQGLLLLVGVGPDDTQEDLDY
                     AVRKIVNMRIFSDEAGKMNKSVQDVAGKILSISQFTLFSDTQKGNRPAFTGAAAPALA
                     SQLYDAFNQALSAFVPVEVGVFGADMAVSLVNDGPVTIVLDTKNK"
     gene            complement(55506..57707)
                     /locus_tag="U1099_000051"
     CDS             complement(55506..57707)
                     /locus_tag="U1099_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEM4866407.1"
                     /translation="MKEINFTGEEVVGLTATYLPAEDVAFVQKALDFATEAHKLQFRK
                     SGEPYIVHSIQVAGILAGLKLDAVTVACGFLHDVVEDTDVTLDDMEAEFGPEVRHIVG
                     GVTKLGKVEYKSHEEQLAENHRNMLIAMSQDMRVILVKLADRLHNMRTLKHLRKDKQE
                     RISRETMEIYAPLAHRLGISSIKWELEDMSFRYLNEVEFYKITRMMNEKRREREELVN
                     EVVDKLRDYTEERHLHGQIYGRPKHIYSIYRKMHDKKKRFDQLYDLIAIRCIMDTPSD
                     VYAMLGYIHELWKPMPGRFKDYIANQKANGYQSIHTTVYGPRGPIEFQIRTMEMHQVA
                     EYGVAAHWAYKRGGKATASEKELRWINNLIELQEGAGDAQTFVNSVKDDIFTERIYVF
                     TPDGAVRELPKDSVPIDFAYEIHTKVGERATGAKVNGRMVPLTTKLKTGDQVEIITSA
                     NSFGPSRDWVNLVKTHKARNKIKQFFKNQDKELSVSKGREMLQNLLQENGYVPNQYLD
                     RRHMDEVLQKTSYKTDESLFAAVGFGEVSAISIFNRLTEKERREAERAKAKAVADELV
                     NGGEVKHDNKDSLKIRHEGGVVIEGASGLLIRIAKCCNPVPGDEIVGYITKGRGVAVH
                     RVDCMNLKSQENYDVRLIDVDWEDENSTKEYMANIDIYGLNRSGLLNDVLKVLTNASK
                     NISSVNAQPTKDMKFATIHVSFGISNLATLTSLVDKIKSVPEVYSVKRTNG"
     gene            57971..60460
                     /locus_tag="U1099_000052"
     CDS             57971..60460
                     /locus_tag="U1099_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEM4866408.1"
                     /translation="MKFRFSKCALTLSLALLASTNNALVSAEETSTDTPVLKTETEIS
                     SSVATSQAEATTGEVATPTTTETTAGEAVTPTTETTDQSVTPVTEEAAPVVETSNTPV
                     NDTTASPVTATRAAATETTTPVEGQSVDVRILATTDLHTNLVNYDYYQDKPVETLGLA
                     KTAVLIEEAKKENPNVVLVDNGDTIQGTPLGNYKSIVDPIEEGEQHPMYAALETLGFD
                     VGTLGNHEFNYGLAYLEKVIRTANMPLVNANVLDPTTKDFLYTPYTIVKKTFTDTEGK
                     KVTLNVGVTGIVPPQILNWDKAYLEGKVIVRDAVEAVRDIIPTMRENGADIVLVLSHS
                     GIGDDQYEVGEENVGYQIASLSGVDAVITGHSHAEFPGTAEKPSFYAKYSGVDDTNGK
                     INGTPVTMAGKYGDHLGVIDLNLVFKDGKWTTTSSKAAIRKIDTKSSVADGRIIDLAK
                     EAHNETINYVRQQVGETTAPINSFFALVQDDPSVQIVNNAQIWYAKQQLAGTSEANLP
                     ILSAAAPFKAGTRGDASAYTDIPAGPIAIKNVADLYLYDNVVAILKVNGAQLKEWLEM
                     SAGQFNQVDPSSTEPQNLVNTDFRTYNFDVIDGVTYQYDITQPNKYDRNGKVVNETAS
                     RVRNLQYNGQDVTADQEFIVVTNNYRANGTFPGVREASVNYLLNLENRQAIINYIIAE
                     KVINPTADNNWTFTDSIKGLDLRFLTADRAKELVANQEGLVYLQASAENEGFGEFKFV
                     YTEPKVATPDEQQSDQGNTGQDIVLESGQRITLPAVNPPAPAPQHKLASPHSQASTKT
                     LPATGEATSMLSLLGLTLIGFVGAWTKKKEH"
     gene            complement(60581..61138)
                     /locus_tag="U1099_000053"
     CDS             complement(60581..61138)
                     /locus_tag="U1099_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866409.1"
                     /translation="MKRLSTPIFAALGFFLIVFVALNQFGRVPGTSYQIISELVLYNQ
                     ATEQTYEFENHEVRKAFIRQLSSTSSRGFQASDSRTSLETTYHETIMSENLISSIYGG
                     EEGRIMEFSRPRKSTLGNLVFASESVSYLVFARKYGNIRLETTILCQEFRVEQRLANQ
                     SNWVDLGTYTVKSPKETRYVPIYWE"
     gene            complement(61379..62094)
                     /locus_tag="U1099_000054"
                     /pseudo
     CDS             complement(61379..62094)
                     /locus_tag="U1099_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636729.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
     gene            complement(62104..63366)
                     /locus_tag="U1099_000055"
     CDS             complement(62104..63366)
                     /locus_tag="U1099_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866410.1"
                     /translation="MSELEQKEHRELIVSDDCFALIQKMSELDSSAYQRYEITNPNIL
                     AHVEKIVQPALKHHLKKAGQPNIPKGLFKVDLPIGDLHKIKGETDKYRAFVKGAEGKF
                     KAHAILTPAQVEIAKGAKAAAAVSGAMQVAAMVVGQYYMAEINGQLDSMKETLNDVKD
                     FQVREFKGTVRALIINISQLSKFSADYVENAELARLKLMECANFRAEVTKLLEQVNLA
                     IEESITKGKGLSFEKYEQLIDKLSTNLFYQQTLLRLLAELSKLDLVFSQGVASEDSSY
                     YVYEYYLNNCNSLGEKIANWHKDKIKFFQIDTKRNRRTQEGPMKVVADIAKELDKNAH
                     VAAGVGFLGGLVGAAAGFGIGKAAGELNKAIRYIDVRKRTVKLISEQETVSYQSSKRL
                     SVNNYGENVKLLVEDGKVYYLLPDDSES"
     gene            complement(63380..64126)
                     /locus_tag="U1099_000056"
     CDS             complement(63380..64126)
                     /locus_tag="U1099_000056"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEM4866411.1"
                     /translation="MQQYFINGRAPQGVFQISDKDTAKHMFSVMRLQADEQIVLVFDD
                     GIKRLARVVDSQNQSVEIIEELSDNVELPVSVTIAMGFPKGDKLEFVAQKATELGMAA
                     LWAFPADWSVVKWDGKKLGKKAEKLEKIAQGAAEQSKRNRIPAVRLFEKKVDFLAQLA
                     GFDQIILAYEEAAKEGEQANLVKILSGLEIGQSVLVIVGPEGGVSPEEVAAFEGAGAV
                     KTGLGPRILRAETAPLYALSAISYATELLR"
     gene            complement(64128..65081)
                     /locus_tag="U1099_000057"
     CDS             complement(64128..65081)
                     /locus_tag="U1099_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEM4866412.1"
                     /translation="MNSWQELTIHVHRDAEEAVSNLMIETGSQGVAISDSADYVGQED
                     RFGELYPEVEQSDMIVITAYYPDTVDIEEVKADLATRLADLTGFGLETGQVSLESQEL
                     AEEDWADNWKKYYEPARISHDLTIVPSWTDYEATAGEKIIRLDPGMAFGTGTHPTTKM
                     SLFALSQVLRGGETVIDVGTGSGVLSIASSLLGAKEIYAYDLDEVAVRVAQENIDLNA
                     NTSNIHVAAGDLLRGVDIKAEVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIADK
                     WDMVRASAEAAGFFLETHMIQGEWNCCVFKKTADRSGVIGG"
     gene            complement(65677..66030)
                     /locus_tag="U1099_000058"
     CDS             complement(65677..66030)
                     /locus_tag="U1099_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEM4866413.1"
                     /translation="MVHEMLLAPKPFEMMKSGQKTIELRLYDEKRKHIQIGDRIRFYC
                     TENQTQTIEVQVLDLHIFDNFAQLYKELDLLSCGYTQSSIRGAKPEDMEDYYSREQLE
                     QYGAVGIELRVIDSI"
     gene            complement(66032..66502)
                     /locus_tag="U1099_000059"
     CDS             complement(66032..66502)
                     /locus_tag="U1099_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEM4866414.1"
                     /translation="MAKHGFLDVLEAALDKHFTYDYELNWDKKNHAVELAFILEAQNV
                     AGIETVDDEGNASSEDIFLEEYVLFYNQDKSRFDEEDYLVALPFDAKKGFSAEFLTYF
                     AGFLKDTADQGLDDLMDFLVDPEAQDFAISWDGEAFEKGRADLLEGEFYPYPRY"
     gene            66576..67862
                     /locus_tag="U1099_000060"
     CDS             66576..67862
                     /locus_tag="U1099_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEM4866415.1"
                     /translation="MPANLALRMRPKSIDEVIGQEHLVGPGKIIRRMIDANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATTDNQKRLQEIAEEAKFSGGLVLMLDEIHR
                     LNKTKQDFLLPLLENGNIIMIGATTENPFFSILPAIRSRVQIFELQPLKTSHIRQALE
                     LALTDSERGFDFPVTIEPEALDFLANATNGDLRAAYNSLELAVLSTKESDDGSRHIDL
                     DAVENSLQKSYISMDKNGDAHYDILSALQKSIRGSDVNASLHYAARLIEAEDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALEAAQKIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRKNGHLPIPNHLRDGHYAGSKELGNAIGYQYPHAYPEKWVDQQYLPDKL
                     LAADYFTANDTGKYERALGMTQEKIKNLKKNRRQNP"
     gene            67964..68157
                     /gene="ssrS"
                     /locus_tag="U1099_000061"
     ncRNA           67964..68157
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U1099_000061"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            68166..68238
                     /locus_tag="U1099_000062"
     tRNA            68166..68238
                     /locus_tag="U1099_000062"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68199..68201,aa:Lys,seq:ctt)
     gene            complement(68343..68900)
                     /gene="nrdG"
                     /locus_tag="U1099_000063"
     CDS             complement(68343..68900)
                     /gene="nrdG"
                     /locus_tag="U1099_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEM4866416.1"
                     /translation="MNNPKPQEWKSEELSQGRIMDYKAFNFVDGEGVRCSLYVSGCLF
                     HCEGCYNVATWSFKAGIPYTKELEDRILADLGQPYVQGLTLLGGEPFLNTGTVLPLVK
                     RIRKELPEKDIWSWTGYTWEEMMLETPDKLELLSYLDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLQEKRVVLWDKLKK"
     gene            complement(69087..69581)
                     /locus_tag="U1099_000064"
     CDS             complement(69087..69581)
                     /locus_tag="U1099_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866417.1"
                     /translation="MELRRPTIADKETILEMLAEFEAAGSAMDGGFISKDVDFEEWIF
                     RNKDYEAGLNLPDGFVPSIQYVSFDQTGRALGFLSLRLRLNDFLLNKGGHIGYSIRPT
                     ERGKGYAKDQLRLGLQEAATKNISKVLVTCSTDNQASRRTIVACGGVLEDVRDGVERY
                     WIED"
     gene            complement(69583..70929)
                     /locus_tag="U1099_000065"
     CDS             complement(69583..70929)
                     /locus_tag="U1099_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEM4866418.1"
                     /translation="MDYTDISILHTNDMHSYMENFPKKAQLIANIRARNEKEGVPTFV
                     FDSGDLFSGNIFFNMYRGVKEIELMNQIGCQAMTLGNHEFDHGDELISRLDDYAQFPI
                     VSSNLRYQDVEVADELVPLEDVLEFDMNGKNLYVLGLTTLETQEVASPSENVIFEDHK
                     QALRRLVDQIVSQNPSAHLVLLSHLGYDEDVHLAKDFPELNVILGGHTHTILKQPSQI
                     GGVSICQAGQYGRFVGHLSLRCFADGRHEVQAYDLIEVEKLTQEDRAVKAIIDQMKSE
                     RDAAFSKPIAILPQALDGERDSIRQGLSTLAPVICQALFEQASQLGLQADGAVINGFG
                     IRASLSAGPIFYSDLVKVLPFSKRVLLVRIKGRDLLSSLQTGLHPQMWGIVQSGEDLL
                     VNGRAVESDKDYQIVTNSFVWSGKDNYDDFHKAEIVQDLGLDIDIISEFFKRQYGG"
     gene            complement(71158..73329)
                     /gene="nrdD"
                     /locus_tag="U1099_000066"
     CDS             complement(71158..73329)
                     /gene="nrdD"
                     /locus_tag="U1099_000066"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEM4866419.1"
                     /translation="MNVQENVLPSIELLVLKRDGRTVSFDQDKIFSALRRANQELEHP
                     VSEAGLKIVLEAVLAEIGRRFEKDVQIYEIQTVVEQELLKAHLYDLAELYIQYRTRRD
                     FRRHQATDINFEIHKLLSKDQSVVNENANKDADVFNTQRDLTAGIVGKSIGLKLLPAH
                     VANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLAQGFKIGNADVESPKSIQTATAQ
                     ISQIIANVASSQYGGCSADRIDEVLAPYAELNYQKHLKDAKDWVLPDKQEEYARAKTQ
                     KDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILNIRIKGLGREG
                     RTAIFPKLIFTVKRGLNLEPDSPNYDIKQLAIECATKRMYPDMLSYDKIVELTGSFKV
                     PMGCRSFLQGWKDENGQDVTSGRMNLGVVTVNLPRIAMESAGDKDKFWEIFVERMAIA
                     KDALVYRVERVKEATPANAPILYQYGAFGKRLAKTDAVDEVFKNRRATVSLGYIGLYE
                     VATVFYGGEWETNPEAKDFTVDIIKKMKALVEDWSDEYGYHFSVYSTPSESLTDRFCR
                     MDTEKFGIVKDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPAVGASGGFIHYCEYP
                     VLQQNPKALEAVWDYAYDRVGYLGTNTPIDHCYACDFEGDFEPTERGFKCPNCGNSDP
                     KSVDVVKRTCGYLGNPQARPMVKGRHKEISARVKHMNGSSL"
     gene            complement(73635..74378)
                     /locus_tag="U1099_000067"
     CDS             complement(73635..74378)
                     /locus_tag="U1099_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM4866420.1"
                     /translation="MIKIRNLVKEFSGQKVLDGIDMDIEKGQVIALVGPSGAGKSTIL
                     RSLNYLEQADAGSIEIDGFTVDFTNISKEEILTLRRKLAMVFQQFNLFERKTALDNVK
                     EGLKIVKRMDDASATALAKEELRKVGLSERENHYPRHLSGGQKQRVALARALAMQPDI
                     LLLDEPTSALDPELVGEVERSIADAAKTGQTMILVSHDMNFIYQVADKVLFLENGRIL
                     EQGTPEELFHHPKEERTREFFASYSNPQF"
     gene            complement(74378..75181)
                     /locus_tag="U1099_000068"
     CDS             complement(74378..75181)
                     /locus_tag="U1099_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM4866421.1"
                     /translation="MIGVLGITGPAWYQGFLEWLPDGKLFSIRAVFDALPSIIERLPI
                     TIGLTLAGAFFGLILGLVFAIVKINRVRFLYPVQAVFVSFLRGTPILVQLMLTYYGIP
                     LVLRGLNQEYGLDWNINAIPAAVFAITAFAFNEAAYMSESIRAAIQAVSPGEIEAARS
                     LGMTSGQVYRRIIIPNAAVIATPSLINTLIGLTKGTSLAFSAGIVEVYAQAQIQGGAD
                     YRYFERYISVSLVYWALSVLIESFGRFLENKMAIQAPESVDISGVGGDR"
     gene            complement(75367..76218)
                     /locus_tag="U1099_000069"
     CDS             complement(75367..76218)
                     /locus_tag="U1099_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM4866422.1"
                     /translation="MKLRTILSLAGITALATVALAACSSTSNSSNTSSSEADKKETIV
                     MGTVGTTKPFSYRDENGELTGFDIELARAIFDGSDQYELTFETTDGSGQFPGLDSGRF
                     DLLGNNTSYTKERGEKYLYSYPTASTPSVLAVPKDTDIQSYDDIGGHSTQVVTGTTTA
                     AQLEEYNTQHTDKPVDINYTGENITQILTNINDGKYDFKIFDAPTVNAIIADQNLTNL
                     KTIELEAAEKPFIYYVFSSDNTALQSFVNDRIKALNEDGTIAKLTEQFFGKDYAPTAE
                     ELAVPNE"
     gene            complement(76251..76937)
                     /locus_tag="U1099_000070"
     CDS             complement(76251..76937)
                     /locus_tag="U1099_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEM4866423.1"
                     /translation="MGKPIIGITSNEKPVAEGIPIMNLSLSRNFADGIKRAGGLPFYI
                     PLSGETDVVDYVSAIDKLLLTGGQHVLPQFYGQEKLIESDDYFKERDEFELKLVHEAL
                     RQEKPILGICRGMQLFNVARGGTLKQAVEGHRSLPFDLIHQVECKEATPLASIFGQRV
                     AVNSIHSQAIENLGQGLEAIVHSVDDGIIEAVRSMDETRFLGVQWHPELLIDQSGGNQ
                     KLFDYFVQTL"
     gene            complement(77051..77224)
                     /locus_tag="U1099_000071"
     CDS             complement(77051..77224)
                     /locus_tag="U1099_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866424.1"
                     /translation="MKIILTIVYSLGVVWNILFLAVNVGRIPVINLLWPAVILVACAI
                     NLYFLWTRKQESE"
     gene            complement(77477..80017)
                     /gene="mutS"
                     /locus_tag="U1099_000072"
     CDS             complement(77477..80017)
                     /gene="mutS"
                     /locus_tag="U1099_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEM4866425.1"
                     /translation="MATEKISPGMQQYLDIKAQYPDAFLLFRMGDFYELFYEDAVEAA
                     QILELSLTSRNKNAENPIPMAGVPYHAAQQYIDTLVELGHKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVTDSSKMGADSNYLVALDRQGVQFALSYMDVSTGQFFVTSLDDFTS
                     LCGEIRNLRARELVIGYALSEEEEQVFSNQMNLLLSFEDEVTEDVQLIDNSLTDLEKA
                     AAGKLLSYLHRTQMRDLSHLQKVVHYEIKDYLQMDYATKSSLDLLENGRTGKKHGSLY
                     WLLDETKTAMGMRLLRTWIDRPLIDLKRIENRQAIVQVFLDYFFERSDLVEALKGVYD
                     IERLASRVSFGKTMPKDLLQLSQTLGNIPAIKNILQQINEPALGNLVAGLDPIPELHA
                     LISSAIDPEAQGTITDGNIIRTGFDETLDQYRLVIREGAGWIAEIEAKEREASGINNL
                     KIDYNKKDGYYFHVTNSNLGNVPDHFFRKATLKNSERYGTEELAKIEGQMLEARDKSA
                     NLEYEIFMRIRQEVEKYIGRLQKLARTIATIDVLQAFAVVAEQQHLVCPRFTDQRELT
                     IDRGRHAVVEKVMGKQTYIPNSIHLNTDTHMQLITGPNMSGKSTYMRQLAVIVIMAQM
                     GSYVPADQAELPIFDAIFTRIGAADDLVSGQSTFMVEMMEANKAVRLATDRSLILFDE
                     LGRGTATYDGMALAQSIIEYIHDKIGAKTLFATHYHELTDLSQTLEHLENVHVSTLEK
                     DGQVTFLHKIAQGPADKSYGIHVAKIAGMPEELLQRADRILQTLENQAPTAPTTPAPI
                     VVEEPSGQIDLFADTPSHPVLDELAKLDIYNMTPMEVMMAVAELKKKI"
     gene            complement(80032..80472)
                     /gene="argR"
                     /locus_tag="U1099_000073"
     CDS             complement(80032..80472)
                     /gene="argR"
                     /locus_tag="U1099_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM4866426.1"
                     /translation="MNKAGRHDLIKAMIRQEKIGRQTDIQQGLEARGVVVTQTTLSRD
                     LRELGVIKIHENGRSFYSLAIEEERVNFIQLLAQYAYKVDRASFILVLHSELGEAALM
                     ANIIDAEKPETILGTLAGANTLLVICRDDVAAQQVEAEINYYLN"
     gene            80579..82267
                     /locus_tag="U1099_000074"
     CDS             80579..82267
                     /locus_tag="U1099_000074"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEM4866427.1"
                     /translation="MNQKQVIAERLAAILPSLEVEAIYNLLEKPKSSEMGDIAFPAFS
                     LAKVERKAPQAIAADIVEKLDTTGFEKVVATGPYVNFFLDKAAISHQVLTDVITEKDQ
                     YGKLNIGQGRNVTIDMSSPNIAKPFSVGHLRSTVIGDALANIHEKLGYKPIRINHLGD
                     WGKQFGMLIVAYKLWGDKAAVEADPISELLKLYVRINAEAEEKPELDDEARQWFKKLE
                     DGDSEAHELWQWFRDESLVEFNRIYDKLDVTFDSYNGEAFYNDKMDEGIQILEEKGLL
                     QESKGARIVDLESYNLPPALIMKTDGATLYITRDMATAMYRKRTYDFVKSIYIVGQEQ
                     INHFKQLKAVLKEMDFDWSDDMTHVTFGLVTKDKKKLSTRKGNIILLEPTLDEAISRA
                     LTQIEAKNPDLENKEEVAHAVGVGAVKFYDLKTDRDNGYDFDLEAMVSFEGETGPYVQ
                     YAYARIQSILRKANFVPSAENDYKLADAESWDIIKHIQNFSNVVERAGDKFDPSLIAK
                     YAINLAQAFNKYYAHTRILDESPERDSRLALAYATGLVLKEALRLLGVKAPEKM"
     gene            82447..83361
                     /locus_tag="U1099_000075"
     CDS             82447..83361
                     /locus_tag="U1099_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEM4866428.1"
                     /translation="MKTFLRRKGIACQLLAAIFIVGINLQSPPSASANARGDDYPYTV
                     VDAVDPWRLYTRQCTSFVAFRLSTVNGFTLPPAYGDANVWGHRAKQEGYQVNMTPAVG
                     AVAWWETPMHVAWVSAVNGDTVEIEEYNYGVRYTYGRRVIPKNAVSGYIHFKNLGAGT
                     TNSQPAVAPSGTYTFTSQLAIKSQASLSSPTLDYYYAGESVRYDKVLEADGQRWISYI
                     SYSGARRYIAIQPTITQSTNNSLPASGSYTIQSYSSVRNNPSLSSPEITFYGAGSVVR
                     YDKVITAEGRTWISYISYSGARRYIAIS"
     gene            complement(83415..83939)
                     /locus_tag="U1099_000076"
     CDS             complement(83415..83939)
                     /locus_tag="U1099_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866429.1"
                     /translation="MKRPIHLYIFVVLSSIASVLRVFNVFFAKYNEEAVRQLLQNFNV
                     EGLDEVYFTYMIESVNFQTNLVNKAFAVVLLLAVIATIVLLFLKKNEQASYTYLGYLF
                     VTLLFSTYAFIGEKGLSQIYTDSVMRQSVEAQAMMNYIIRVVLFAIYFGVTVFFHLRK
                     PKEKPSTAINSTDI"
     gene            84051..84233
                     /locus_tag="U1099_000077"
     CDS             84051..84233
                     /locus_tag="U1099_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866430.1"
                     /translation="MKKLPTYIGVIIFVVTLLINWLLPSLAKVLNTFFIWTIVACLLY
                     TIYYWIRYWLYVRQQR"
     gene            complement(84274..85791)
                     /gene="malQ"
                     /locus_tag="U1099_000078"
     CDS             complement(84274..85791)
                     /gene="malQ"
                     /locus_tag="U1099_000078"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEM4866431.1"
                     /translation="MKVRQSGVLMHISSLPGKYGIGSMGQSAYEFVDFLVRTRQRYWQ
                     ILPLGTTSYGDSPYQSFSAFAGNTHFIDFDILIDKGWLAAEDLHGVDFGQNPTEVDYA
                     AIFYARRPLLEKVVVNMKAQGLPEDYWMFLGENDFWIHTFAEYMAIKEHFDLKPWTEW
                     EDQGARLRYHDTLEYYRHLLADRIDYHRITQYLFFSQWKALKAYANAKGVEFVGDMPI
                     YIAADSADMWANPHFFKTDEEGKPSVVAGCPPDAFSEIGQLWGNPIYDWEAMKHDGFT
                     WWVARLRESFKIYDMVRIDHFIGFASFWEIPAGEETAVNGYRVEAPGFELFDTIKREL
                     GDLNIIAEDLGSVTDKVIQLRDTTGFPGMKVLQFAFDPEGDSIEMPHNHPNNVVAYTG
                     THDNDTILGWYENETTKESRAFLDKYSNRREGETVSHALFRLLFGSPAFMAVATMQDL
                     LGLDGSARMNLPNTLGGNWTWRMTADQLTPEIEAELLDLTETYRRVNVHLVDEKS"
     gene            86095..87354
                     /locus_tag="U1099_000079"
     CDS             86095..87354
                     /locus_tag="U1099_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM4866432.1"
                     /translation="MKHNLLKSVALLAASTAVLAACSNSGSSTEASKSAEGSKELTVY
                     VDQGYESYINDVKAGFEKENGVSVTVKTGDALTGLDNLSLDNQSGSAPDVMMAPYDRV
                     GSLGSEGQLSELTLADDSKADDTTTALVTNGGKVYGSPAVIETLVLYYNKDLLTEAPK
                     TFAELETLAKDSKYAFAGEEGKTSAFLADWTNFYYTYGLLSGYGGYVFGENGTNPKDI
                     GLANEGAIKAIEYAKTWYEKWPQGLQDGTAANNLINTQFTDGKAAAIIEGPWKAASYK
                     EAGVNYGVATIPTLVNGKNYSAFGGGKAWVVPAGAKNQEMAQKFVDFLTATDQQKALY
                     DATNEVPANTEAREYAVSKKDELTTAVINQFASAQPMPNISEMGSVWTPAGNMLFEAA
                     SGSKDAKTAATDAVKAIADEIAQKHSN"
BASE COUNT        25006 a        20858 c        16997 g        24569 t
ORIGIN      
        1 gcaatcaaaa aaaagcgtag ctgattacta cgctagtact tgcctgctga actcattaaa
       61 atttttaggt tgtcaaacgt taatttgata accttttttc attgtaaaat ttgttcacct
      121 tcttgttcac ctttggaggt gttctaagga aatctaaaga gatacgattt tttaaaaacc
      181 cagtaaaatc acgagtttgg gagttatgac actctcaaac gtgagtcaaa tttcaattcc
      241 acggattttc atagtagttt cccttcaatt tcttcctgca taatacgctc tctattataa
      301 tttattcgta ggctaaaagc aaaactccaa cctagtggct ggggttttca actttactca
      361 aacacaaact ggttgtggta gagttcggca tagaagccgt tgagcttgag gagttggctg
      421 tggttgcctt gctcgatcac ttttccgtct ttgaggacga tgatttcgtc cgcattgagg
      481 atggttttga ggcggtgggc aatgacgaag ctggtccgtc cagcgataat agcctccata
      541 gccttttgaa ttttggcttc cgtcaccgta tcaacgtttg aggttgcttc gtccaaaatc
      601 aagacttgtg gatctgtcaa gagcgtacgg gcaatggaaa tcagctgttt ctgacctgtc
      661 gagaagatat tctcatcatc agtcacaaag gtttcataac cctctggcaa gctcatgata
      721 aagtcgtgga tatgggtggc acgagctgcg gtttcaacca tttcctgcga aatgctctca
      781 tcaccaaagc gaatattgtc cgcaatagta cctgagaaaa gaaccgactc ctgcaagaca
      841 atgccgacct tatcccgcaa gctgtccaag tcatattccc gaatatcacg accgtcaaaa
      901 gccacactac caccattgac atcgtagaag cggttaatca agttcataat ggtagtctta
      961 ccagatcccg tcggaccaac gactgccacc attttgccct taggagcaga gattgacacc
     1021 ttgtccaaga ccttctgacc cggcaggtag ccaaagtcaa tgtccttgat ttcaacacct
     1081 tcttttaatt cgataaatag cggagcgttt tgagggcgaa cttcctcagg ctcatcaaac
     1141 atttcctgaa tacgatgagc tcctgtgaaa gctagctgca attctgccca gctggcagca
     1201 acctgcatga ttggctggta atactgttgc gaatattgaa caaaagtcac caccagacca
     1261 agtgcggcag ctgtttctag cgaactatcg ttgagaacaa tgctggaacc tgcaaagata
     1321 acaatggcag tattgaccaa actcatccca ttcataaatg ggaagagaat cccaccaaac
     1381 aagcgtccct tgaaggtcgt acgacgaact tcttcattga gttccaagaa accatcaatt
     1441 gtctcttcct gcacaccttg tacaataatc gctttttgtc cggaaatctt ctcatccatg
     1501 tatgcattaa gcttagatac cgcagcctgt tgcttatcag tatatttccg tgacaggcga
     1561 atgataatca ctagggcaat caaagcaact ggcgtagaag caatggtcac caaggccaag
     1621 cgagtatcct gacggaacat catgatgacc ataccaacat aaagagcgat gttggtcacc
     1681 acttgggtca aggactggtt aaaagcgttt tggatattgt ccaaatcact ggtaaagcga
     1741 gaaagaatat ccccgtcctt gtggctatca aagaaggcaa ctgtcaagcg ttccagcttg
     1801 ccaaacaagc ccttacgcat acggttggtc gaatgagcca cgatacgcgt gaagaggagg
     1861 gtgtaaatca aggaagccac aaccgtcgcc gcataggcaa agaaaagatt gagcataacc
     1921 ccgttaaaat cagccatatc tggctgaaag tcagcctgac cagcttgatt ggccataaag
     1981 taagcctgcc caattttccc catctccgca atggcatttc caaggaaaac aggtgtcttg
     2041 acctgcaagt aggtcgctgc cacaatagct agaaaaatca cagcaaagga cagtttatag
     2101 cgtttaaaat aaaaccagaa aaaacgtaat gtcttcatct attcctccct ccctttctgt
     2161 gtttcgtaga tttcacggta aacatcgttc gtcgctacca actccgcgtg ggtaccttga
     2221 ccgatcagac ggccttcgtc aagaaccaaa atcttatcag ctttgacaac agaagaaatc
     2281 ttctgagcaa cgatgaccgt cgttgtcccc ttcaagtcat ggttgagggc ctcttggacc
     2341 agcttctcag acttggcatc caaggctgat gtcgaatcat ctagcaccaa gactttagga
     2401 tcactaatca ccccacgcgc aatggacatc cgctgtttct gaccacctga gaagttattt
     2461 ccacgttctt ctacttggct ctcataggta tcatccaagc ggtcgataaa ttctttggct
     2521 tgggcaatac ctgcggcccg ctccaaacgt tccaaatctg caccaggtgc accttgacgc
     2581 aggttgtctg caatggtccc tgagaagaga atagcttttt gcaaaacgat agacactgta
     2641 tctctcaagg tttcctgact gatgtctttc aaatcacgac cgccaattga cacagtccct
     2701 tcctgtggat caaacaaacg cggaatgagc tgagctagtg ttgatttacc cgcacccgtt
     2761 gcaccgacca caccgaccat ctgaccagac tcaatctcaa aggagatatt tttcaaggtc
     2821 ggctcggtat catgcggata ggtgaagcta acattgtcaa agacaatccg accatccaag
     2881 tcctcttttg caccatcttt aaaggtcatc gctggctctg tatctaaaac ctcactcaaa
     2941 cgtttaatgg aaatagcagc acgtgaagcc tgcatcccca taaagctagt catgataatg
     3001 gcaaacatga tttgcatgag gtaactcata aaggaagtaa agcccccaac tgcttcgata
     3061 ttagtgtcta acatccctga aacgagataa agcgacacaa aaatcgccat gtaggagata
     3121 aacatcatca aaggttgcaa gatagaaaaa ccataaccaa taaagaggtt aagatccaaa
     3181 agctcattgg atacttcttt aaatttagcg tattgattct tctcctgtac aaaggactta
     3241 accacacgaa tcccacggag gttttccttt gcaatgctgt tcatcctgtc catcagggac
     3301 tggaatttac caaagcgcgg tcccatcaag cccatcacga ctgccatgat tcccatgatg
     3361 atgatgacca tgagcacgat aatccaccaa agttctggca aagtgcgcac cgccatgata
     3421 aaggcaccaa caaagagcag aggtacccgc atcaagatcg taaagagcat catgacaagg
     3481 ttctgaattt ggttgacgtc gttggtcata cgaaccacca aattccctgc gttaaattcc
     3541 tctacattgg cataagaaaa gctctgaatt ttccgaaagg tcttctcacg aatatccgca
     3601 gacactccct gagaaatctt agctgccatg attgtattgg tcagaccagc caagagaccg
     3661 attcccgcaa tcagcaagag gaacttaccg acacgaacaa tttcttcctt atcattggcc
     3721 aagacagcag tcaatacatc ctgtaaataa ctaggttgca tcaaggtcgt tgctacgaca
     3781 atagacgtca acaagaccga tgccaaggca taccatttgt aacgtaaaat ggcttcttta
     3841 aacatgattc ttctccttat agtgttgaat attatctttt aattgatcgg ctacctgtct
     3901 gatcaaatca aattcttcct tttgaatacc tcgaaagaga gaatggtgca tctcctcatg
     3961 gaaggatttt aggtgacaaa tcttgctccg acctttctct gtcaagacca actgcttata
     4021 gcgtctgtca aactgagaag gtaaaacccg aataaagtca tttttctcca ttcgcttaac
     4081 tagattgctg gcaacggatt tggaaatcct caactccgct tcaatgtctt tgacaaaggt
     4141 ttcaacttca gaccgttctg ctatgaaacg cagagcccat ccctgtggtc cagctaggtg
     4201 ctccacatca tattcctttg caatggtttc actgatttgt tcaatctgat ttaatagatt
     4261 ccgaaaatct gcaatagtat gtcccataat agctcctttc tacgaaaata attaccatga
     4321 gaactattat agttctctta ggaactattg tcaattattc cgtttgctct tttctgaaaa
     4381 tattttgtta tttcagcacc attacttact cgaaaactat aatatatata ctgcataact
     4441 tatataagat tttatgtatc aattatggta taatatatct aaattacacc aactagaaag
     4501 gattttttat gaccaaacaa accattgcca tcttgggacc cggttcatgg ggcaccgccc
     4561 taggacaagt tctcaacgac aacggtcata ctgtccgtat ttggggaaat gtccccgaac
     4621 aaatcgacga aatcaataaa gaacatacaa ataagcgcta tttcaaggat gttatcctag
     4681 atgaaaacat caagggctgt aaggatttga ctgaagcctt ggacggtgtt gacgctattc
     4741 ttttcgttgt accaaccaag gtaacacgtc tcgttgccaa gcaagtcgca caagctctca
     4801 aacacaaggt agttgtcatg cacgcttcaa aaggcttaga gcctgatagt cacaagcgct
     4861 tgtctgaggt actggaggaa gaaattcctg ctgaactccg ttctgaaatc gtcgtggttt
     4921 cagggccaag tcatgcagaa gagactatcg tccgtgattt aaccttgatt tctgcagctt
     4981 caaaagactt ggaaacagcc agctacgtcc aaaatctttt cagcaaccac tacttccgac
     5041 tttatacaaa taatgatgtc atcggtgtag aaacagcagg tgcccttaaa aatattattg
     5101 ctgtcggcgc tggcgcttta cacggacttg gttatggtga taatgccaag gcagctatta
     5161 tcgctcgcgg tttgacagaa atcacacgtc taggcgttgc tatgggcgca aatcctctga
     5221 cttacagcgg tctttctggt gttggtgatt tgattgttac cggtacatct gtccactccc
     5281 gcaactggag agctggagat cagctcggtc gcggtgaaaa actcgaagat gtagaacgca
     5341 atatgggaat ggtgattgaa ggtatttcaa caactaaggc agcctatgag ttggctcaag
     5401 aattagatgt ttacatgcct attacccaag ccatctacaa ggtcatctac caaggtgcag
     5461 gtattgaaga tgccatcaaa gaaatcatga cgggtgaatt ccgccatgaa aatgaatggc
     5521 attgattctt attaaacaca aaaaaataaa ttataaagga ttacatactt atgaaaaaag
     5581 ttagaaaagc tgttatccca gctgcaggtt tgggaacacg tttcttgcct gctactaagg
     5641 ctcttgccaa ggaaatgttg ccaatcgttg acaagccaac catccaattt atcgttgaag
     5701 aagccctccg ttcaggtatt gaagatattt tggtggtcac tggtaagtca aaacgttcta
     5761 tcgaagacca ctttgattca aactttgaat tggaatacaa cctcaaagaa aaaggcaaaa
     5821 atgatttgtt gaaactggtt gatgagacaa caggtattcg actacacttc attcgtcaaa
     5881 gccatccacg tggacttggg gatgctgttc ttcaagcaaa agcctttgtc ggaaatgagc
     5941 catttgttgt catgctcggc gatgacttga tggacatcac agatacaaaa gctgtcccat
     6001 tgaccaagca actcatgaac gactatgaaa aaacacatgc ttcaaccatt gctgttatgc
     6061 ctgttcctca tgaagaagtt tctgcatacg gtgttatcgc tccacaaggt gaaggtgtca
     6121 acggtctcta tagtgtggaa acctttgtgg aaaaaccaaa accagaagac gctccttctg
     6181 accttgctat tatcggacgc tacttactca ctcctgaaat ctttgagatt ttagaaaacc
     6241 aaaaaccagg tgccgggaac gagattcaat tgacagatgc cattgatact ctgaacaaaa
     6301 cgcaacgtgt tttcgctcgt gaattcaaag gcaaccgtta cgatgttggg gataagttcg
     6361 gtttcatgaa aacttctatc gactacgccc tccaacaccc tcaagtaaaa gatgatttga
     6421 aacaatactt aatcgatctt ggtaaaaaat tagatacaaa gaaaaactag caatcgctag
     6481 tttttttctt atatcaaata gaatatccct agtagaaaaa cgatcaagct catatagcca
     6541 atgaagctga aaaaaatctg ttttcctgta aagagatcct tttccacaag agggggaagg
     6601 aaaatcacga ccaaacaacc accaatagct cctccaatat gtcctgccat gctaatggtt
     6661 ggattaaaaa gtcccaagac caagttcaat cccaaaagaa ccatataacg ttgaccgaga
     6721 acctgtagat aagggctgcg actaaatttc ctcaaaagtg ccatagctgc aaaaagacca
     6781 aacagagagg tcgatgcacc tgcaccaact acatctggtg taaagaagag aacaaaaaga
     6841 ttgcccatta tcccagacaa gagatagagg agaaagaagc gccgtgaccc aaacagtcct
     6901 tctaattgat aacccaaacc aagcaaggta atgctgttaa agataaaatg ttcccaacca
     6961 atgtggacaa agataggaga aatcaagcgc cacaactgag tcggatcata ccgcaccact
     7021 tgaccgtaca taccaccgaa ttcaaaaatc gtataggctg tagtggtctg cccaaatcga
     7081 aatatttgta tcaaaagaaa aacaagagcc gtgactgcca acaaggcatt ggtcacagga
     7141 tagcgcttat caaaaaagtt cttcaaaaat caacaactcc tttactggaa tatcaaatac
     7201 atctggttca aaatcataga cttgaaagtc tgccaaggtc gataccgtct ccccttgata
     7261 atctgctagg tagcgatcat aaaaaccgcc tccataaccc actcgaaaac ccgcttgatt
     7321 ccaagctaaa ccaggaacat ggatcaactt aaccacagac ttatccacag gttgtgaata
     7381 agtcccaggt tcccaaactc caaacctaga tttcatcaaa tcatcaggat tatattctac
     7441 aaaatccata cgaccttgtg aataggtctt agggatgaaa atctgcttgc catccttttg
     7501 agcctgctca atcaggtaag aagtgtcaaa ctcatggggc atggaaaggt aggttccaag
     7561 cgactttgag cacttatagg tatcagatga aaggagcctt tctgttagtt gctgactcca
     7621 tcttgctcgc tggctactag tcagggcttt caatctctgc aagacctctt gtcgaatttc
     7681 ttttttatcc acaggaactc ctttcttttc agaaaaagac tggaaactcc agtctagttt
     7741 gctttcattt tcaaaaaatc gctcaactta tccacagcaa agggaagcac ttcctctttg
     7801 ggactgagac gtggattgtg aaggggataa ggcgtatcca cacccaacca gaacataaca
     7861 cccggaacct tgttaagcag gtagccgaag tcctctcccg tcatggcagg tggacaatca
     7921 atcatctcca ctccgtcaac accatcaaag taggtcatca gttcatcagc cagctgggga
     7981 ttgttctcca caggcagata accgctagga ttaagctcga tctccaagtc cactccaaag
     8041 gacagggcga ttccctcggc cacttcccgc acccgttttt gcaccaagag gctcatgttc
     8101 tgagtcaagg cccgaatggt cccgtgcaaa aaggccgttt cagcaatgac attgttggtc
     8161 gtgccagcat gcatggagcc gaaggtcacc accgctccct cgatcgggtc aacgttacgg
     8221 ctgaccaccg actgcacctg agtcacaaag tagctggctg ccaccaaggc gtcattggcg
     8281 gtatggggaa atgctgcgtg gccacccttt cctgtaaacc gtatcttgac ttcacaggtt
     8341 ccagcaaaga gggtcgcacg gttggtggcc atttgaccga ctttgaggtc aggtcggaca
     8401 tggagcccat aaaattcatc tggcaaccaa tccccgaaag cccccgcttc atacataagc
     8461 ataccacctg ctagattttc ctccgcaggc tgaaacaaga agagaagatt atttctcggt
     8521 tgctgctctg ccattttctc caaaagcccc agggcaatgg tcatgtggaa atcatgtccg
     8581 caggcgtgca tccggtctgg gtggagggac ttgaagccca gctccgtttc ttccacgata
     8641 ggcaggccgt caatatctgc ccgccagccg atggtctttt caggagcaga acctgtcaga
     8701 tagaccagga tacctgtctt ccatgtacgg acttgggcaa aagagcaatc ttccaacaag
     8761 ttctcaatag tttccataag aaaggcgtgt gtcttgaact cctccatacc cagctctgga
     8821 atctggtgga gggcccggcg tgtcgcgatt aaatctagca tagctacctc ttagagtgtc
     8881 cgcaaagcct cttccaaggc tgttttttgt tgggtttgag cgtcgatttc cttgatgatg
     8941 cgggctggca cacctgccac cactacgttt tcagggacgt cttgggtcac aatagcaccg
     9001 gctgcgacaa cagagccact accgatttgt actccctcga tgacaacggc attggcaccg
     9061 accaaaacat tgtcgccaac acgcactggc tcagccgaag ctggctcgat aacacctgca
     9121 aggaccgcac ctgcaccgat gtggctgttt ttaccaaccg tcgcacgacc accaaggata
     9181 gcacccatgt caatcatggt acctgggccg atttccgcac cgatattaat gaccgcaccc
     9241 atcatgataa cggcattatc accgattgtc acttggtcac ggataatggc acctggctcg
     9301 atacgggcat tgatgttacg tttgtctagc aaaggcacag ccgaattgcg gccgtcttgc
     9361 tcaaccacgt agtctacatt ttcagtcaag tttgtcaaaa gtggctcgac gtctttccag
     9421 tcaccaaaga ggacgttgcc aagttttgtc acttctgcag gaacaggacc agccaattca
     9481 ccttcaaaag tcactttaac agttgtcttt ttcaccgcat taccgataaa ggcaatgatt
     9541 tcttgtgcag tcattttttg tgcagtcata atgattccaa tctaaaaata atattttttc
     9601 tattatatca aaattttctg aaaatttggg aagaaatgca aaaaaatgga aaagaggccc
     9661 tcagttccca atgttattaa gttagctaac ttaataacat tgggcaggag ccaaatcttt
     9721 ctctgttatt catttaataa tgtttgtgcc tgttcatgca caactgtcat cgtttgtttt
     9781 agttgatcac gaactgtttt taaactatcc aaaacagtag ctcgtgcttc ttctggaatc
     9841 actgtcgagt catcccctaa tgtcgcaaat tgtgaatcta gtgctgattt ttgttgttca
     9901 agcatcgtta caatttgctc acgatatggt tttaaagtag tctgtgcttc ttctggtaac
     9961 tgaacaatta acccatcaaa gtcagcaaca taagagtcca tcaatgagtt caatgatgtt
    10021 ttatattctc cgatagtctg aatcgcatca atttctgcat ctgccagata agtcactgtt
    10081 gactctttta aagtaacagt cgaggattca gatgtagact ctttagtaga ggatttcgtt
    10141 gtggatgttt gttccgttga agagcttttt gacgttgttt cttccttact attagagcaa
    10201 gccaccaaac tagtaactgt tagaagagct gagaaaatca gcaaccattt ctttttcatt
    10261 ataaaactcc ttttgtttat tactactaga taatattatt tttaagtatg aaagtcaagt
    10321 atatctagaa aacaatttat ctttttagaa tatacaacca gtagccctct caaatgcaga
    10381 atcattatga gaatcactag gtttttatag ggtatttcat accgttcctg actaaacact
    10441 cggataaagc ttgataaaat ctgccggatt gactagaaat tttataccga acaaattggc
    10501 atattcatct cctaaaaatt gttaatatcc ctagtatata ttaccatgca attttgtaaa
    10561 tttttacagt aagctactgg gtcgactcca tacagcccac tgcgtaatct ctaaccaatc
    10621 cctgttgatt caagactaga ccatgcagaa ctccgccgta aacagctcct ccgtcaatcc
    10681 ccattttccc atctgaactg gtccagattt ggctaatgcg gagcttggtc tggtagaggt
    10741 tataaacagg ggtatgccca aagacaatca ttttccctgt ggtattttcg gcctcatgga
    10801 aaggggtacg taaccaaacc ttatcatgat tactagtttt tcgccagtct ggcaaagtca
    10861 agtccacacc agcatggaca aagatatatc cttccgtttc cagatgatag ggacaagatt
    10921 tgacaaaatc aatcaacttc tcatattgtt ccatcactgc attagcatcc accaaaccat
    10981 ctacaggagc atccaaggga cgacctaata gcgagttgat ggtcgtatct ccaccattcc
    11041 tccggtaatg gtcatagcgt tccaagggat cccgcaacca agccaaaaac attcgttcat
    11101 gattcccagt cagacagata gcatcttttt ctcggactaa ttgccagacc aactccaaac
    11161 aagcttttga attttctcca cggtcaatta aatccccaag aaaaatcagc tgttgtcgtt
    11221 cttcattcca tttcttcaaa atatcgagta gcagttcaaa ctttccatgg acatcgccga
    11281 ttacaaaaaa atcttttttc atagactcta ttctaccata tttaataagc aaagaaaaac
    11341 tgaccgtttc cgatcagttt cattctatta caaacgtgcg ttcaattctg caccgagttc
    11401 ttcaaatcct ggttttccaa gaagagcgaa catgtttttc ttgtaggctt ctacacctgg
    11461 ttggtcaaat gggttgatcg cattgaggta accagagata gcaatcgcca actcgaagaa
    11521 gtagaaggtg taaccaagtg tgaactcatc ttgttgcgga agggtcacaa acatgtttgg
    11581 tacgccacca tctgtgtgag caagaagcac gccgtccgtt gcttttttgt ttacaaagtc
    11641 aacatcctta ccttgcaagt agccaagacc gtcaaggtcc tctgccatgt caggaatgag
    11701 gatatttttt cttggtttgt caacacgaac cactgtctca aagatattgc ggtttccttc
    11761 ttggatgaat tgcccaagag agtgcaagtc tgttgagaag ttagcagatg ttgggttgat
    11821 acctttttgg tccttacctt ctgactcacc agccaattgt ttccaccatt cgctgaagta
    11881 ttgaagtgat ggctcgtagt tagccaagat ttctgttaca tagccttttc tgtaaaggat
    11941 gttacggaca gctgcatatt ggtatgcttg gttttcagca atcttatcag atgtaaatgt
    12001 cgtgcgggct gcgtttgcac cttccataag ggcatcaatg tctgcaccag ctgccgcgat
    12061 tggcaaaaga ccaactgcag tcaagactga gaaacggcca ccgacatcat ctggaacaac
    12121 aaaggtttcc caaccatttg cgtccgcttc aaccttaaca gcaccttttg ccttgtcagt
    12181 tgtcgcatag atacgcttgt tagcttcttc ttgaccgtat ttcttgacaa gtaattcttt
    12241 gaagacgcgg aaggcaatag caggttcagt tgttgtacct gattttgaaa tcacgttaac
    12301 tgagaaatcc ttatcagcaa cataatccac caagtcagca agatagcttg atgagataga
    12361 gtttccagcg taaaggattt gtggagcttt gcgctcttca cgtgtttgca agtttacaaa
    12421 ggcattgctc aagaaatcaa tagctgcttt agcaccaagg taagaaccac cgataccgat
    12481 aactaccaaa acttcacttt cattttggat tttagctgca gcttccttga tacgagcaaa
    12541 ctcttctttg tcgtagtttt ctggcaaatc caaccagccg ataaagtcgc taccaggacc
    12601 tgttccttga cggagcattt ggtctgcaag ggtaacttgt ggttgcatgt attctacttc
    12661 ttgagctcca acgaattggc ccaagacttt tgaataatca aatgtaatat gtgtcatgtt
    12721 tttcctccat ttatgtacca taatatgata acgctttatc atattttttt caagaaattt
    12781 atcaattttc atttattttc atgaaatatg ttgtataaat cttaattttt gtgaaaatct
    12841 aacgaaattt tcgcgtttgt aataatcctc caaagtctct cccgactggg ttactgtaat
    12901 ccatttcggg agaatcttag atagtaggta aagcggaatg agggcaggat accaaatgaa
    12961 catgacttca aagatgaaac ggtcgttgtt gcctcttgaa atgtaacgtt ggcggtattc
    13021 ttctttgaga gaagggtggg gaatatagat atgaaatggc acaccaccgc gtatagacat
    13081 ggccagcaac attctcatca gaaaattcag cgctactagg actgtctttc cttgtctatg
    13141 aagagtgtag gccttattga catagattgt tttatagcct ttatccttca gtgtgcgttt
    13201 ggtggatttt cgctcctctt tcgttaaatg acgaacactg ctattatcgt acttaatgtc
    13261 ggacatttct aagtccacaa catctgtata tttcttagca agtgtcgttt ttcccatacc
    13321 tggaaaggca aataaaaaca taggctttcc tccttattcc tcagtagaat agcagagcat
    13381 ccaaaaagtt ttcttaagtt gcccctattt tgcccctcat ttctaaaaat aaaccaaaga
    13441 aaaaccctct gaaatcctga tttcaaaggg tttttagatt atatgcaaaa agagcacaca
    13501 ttcgacctcg cttagggctg ctggattcct cccctgaccc gcttcacgca cgaatgttgc
    13561 tccgtttaat attataacac aatttggcca gagtgcaagt ttggaatatt aactagctat
    13621 ccgaagttgc ttcctcttgc gtcttcatga taagtttagc agccttctgt ctttctctgc
    13681 gttgtctgaa aaaatcttgc atgatattgg cacattccgc ttctaaaata cctgtttcta
    13741 ccttgacacg atggttgagc cgctcatctg tcagaatgtc atagaggctc cccgcagctc
    13801 caaacttttt gttggttgct ccatagatga cctgcggaat gcgtgctagg ccaatagctc
    13861 cgctacacat cacacaaggt tcaatcgtca caaaaagggt cgaatcaagc aagcgccaat
    13921 tcccctcaac attgttagcc tcttggattg ccataacctc agcgtgcatg attgcctgat
    13981 tgagttcctc acgcgcatta tgccctctgc caataatctg gccatccttg acaatcacac
    14041 aaccaattgg aatttcatcc ttttctagcg actttctagc ttcctgcaag gcttgagtca
    14101 taaaatactc tttttcttca tgtgtatagt tcataaatct attatagcac agccctgata
    14161 acagttcaaa atcacttctt tattctctgc tatcaaaatc aatctaacac tacttcttcc
    14221 ttatcacaca tttctttcaa aattctgccc ttctattaac ttaaaaatat aaaaatacct
    14281 cctcagtcga gaaggtaggg ggagcaatat tgatatttac agatattaat atatcatcta
    14341 ttgtcgatat tgtcaagaaa atttttaaaa aaattcaaaa acagcctcga aaggctgtct
    14401 ctgacatttt ctattacaat tcgccaacta atttcacaac attttctact gtgaagccgt
    14461 agttgtcaat aactgtttgg gctggagctg aggcaccgaa tgtgtcgata ccaaggactt
    14521 taccgtcaag accgacatac ttgtaccaac tttgagttgt acccatttca attgccaagc
    14581 gacgacggat agcatttgga aggatttctt ccttgtaagc tgcgtcttgt gcatcgaaga
    14641 gttcagttga tggcacagaa accacacgaa ccttagtacc tgctgcttct agttctttgg
    14701 cagctttaac agccaagttc acttctgaac cagatgcaag aaggattgta tcaaaccctg
    14761 ctacttcata gacaacataa gccccttttg caaccttgtc aaagtctgtt ccttcttcaa
    14821 ctgtcaagtt ttgacgagtg agaacaaggg ctgatggtgt agacttgcta gtcaatgaca
    14881 agtaccaagc tgcctgtgtt tcacgcgcat ctgctgggcg gaagacattg aggtttggca
    14941 tagcacgaag tcctgccaag tgctcaatcg gctcgtgagt tggaccatct tcaccaactg
    15001 cgatagagtc atgagtgaag acataagtca caggaagtcc ttgaagagct gacaaacgaa
    15061 ctgctgcttt tacatagtct gagaagacaa agaaggtacc accatagaca cgaagtccgc
    15121 cgtgggctgc cataccattc aagatagttc ccatggcaaa ttcacgcaca ccgaactgaa
    15181 tattgcggtt gagtggattt actgaatctt gcaaaccatc ttccttgatg taggtcatgt
    15241 ttgagtgagc caagtcagct gaaccaccta agaaagttgg caatacttta gccgctgcgt
    15301 tgagggcatc ttgtgatgag ttacgagttg cttgtgagaa gccattttcg taaaccggga
    15361 agtcttctgg cttaatttca actacgtcac gaccttccaa gatagctgtt acttcagctg
    15421 ctaattctgg atgtgctact ttgtagtctt caactaattt cacccaagca tcgtaggctg
    15481 ctgcaccgcg atcagctaca ttagctttga agtccgcata gacttcagct ggaatttcaa
    15541 atggtgcata gttccaatca agcgccttac gagttgcttc tgtttcttct gcaccaagtg
    15601 gagcaccgtg aacggcattt gtaccttgtt tgtttggcga accatgaccg ataacagtct
    15661 tgatttcgat aagagatggc ttacctgctg ccttagcttt ttcaatagca gcaaagatgg
    15721 catctacatc tgtaccgtct gttacgaggt ctgtatgcca gccataggca ttgtaacggg
    15781 cacgtacatc ttctgtgaag gaatcttttg tttcgccatc caagttgatg tcatttgaat
    15841 cgtaaagaac gatgagtttt tctaattttt gaagacctgc gtaagaagcc gcttctgctg
    15901 aaacaccctc catcaagtcg ccatcaccac agattacgta agtatagtgg tcaaagattg
    15961 ggaaaccatc gcggttgtac ttagctgcaa ggaaacgctc tgcttgtgca aaacctgtcg
    16021 cagttgagat accttgtcca agaggacctg ttgttgcatc tacacctgct gtgtgaccaa
    16081 attctgggtg acctggcgtt tttgatcccc actgacggaa gttcttgatc tcatccatac
    16141 tcacttcttc aaaacctgaa aggtgaagaa gtccgtaaag caacattgac ccatgacctg
    16201 ctgataagat aaagcggtca cggttcaccc agtttggctg agctgggttg atacgaagtt
    16261 ctttagtaaa aaggctgtag gccattggag ccgcacccat gacgacacct gggtgacctg
    16321 attttgattt ttcgatggca tcaataccaa ggaaacgaat cgcattgact gaaagatttg
    16381 acatgttttt ctcctttttt aaagtcatga atttattata ccataaagcg ttttctatgt
    16441 ctgtttgaaa tgaagcgaga aataaattgt ccgtagaaaa gaggcaggac tttcgtcccg
    16501 cccttggagt gaacagtagt aattcgcaca tttcatactt tatcagagtt tatagtgcct
    16561 ctcctcgtct ctatggggca agagagttct aaatcttata acaaagcctt aaattgaata
    16621 tttgagtctt ctaagaagca atcatcaaag ccacgtgggt ggtgatattc gatacggtcc
    16681 ttgtccatcg gataagtata cttgccacca acttcccaga tgaatggatg gaaatcgtat
    16741 tgcaagcgtt ccttacgcat tttccagagc tgcaagattt catctgttga cgccatgaag
    16801 ttagaccaga tatcgtagtg aacagggatg attaccttag cacgcaagtt ttctgccata
    16861 cgaagaaggt cgattgatgt catcttgtcc tggataccga ttgggttgtc accgtagttg
    16921 ttgatagcaa cgtcgatgtt gaagtcacga ccgtgttttg caaagtagtt tgagaagtgg
    16981 gagtctgcac cgtggtagat ggttccgcca ggcgtttcaa agacatagtt gactgccttg
    17041 cgagccatga gttcatctgt cacaggaagt cctgctaatt taccaccatt ttcgtcataa
    17101 ccttcgattg gtaaggtcac caagcaagta cggtcgaagg attctacagc gtggaccttg
    17161 atgtctttga actcaaagct atcacctggt gcgatacgga tgatacggtc agctggaaca
    17221 ccccattttt cccaaatgtc accactttca accggtccga caaatttgac gtggtccaac
    17281 tttggattgt tgatgatggc agcggctgtg ctgatgtcca tgtggtcact gtggacgtgc
    17341 gatactagat agtagtccaa ttcattgatg gcaaatggat cgataaccat tggctgcaca
    17401 cgcaagtttg gttgcaattt gcgcacaccg gccatgttag ccatctggtg tccccaaacc
    17461 atatctttca cttttttagt ggattttcca cgtgatgacc agaggtccat gaccacgtta
    17521 gctccacctg gtgttttaat ccagacacca cagtttccaa gccaccacat agcaaagttg
    17581 ccttctggca ctacttcttc ttcaatttct tcattgagcc aagttcccca ttctgggaag
    17641 gttgaaagaa tccaggattc tcttgtaata tcttgaactt tagccatttt ccattcctcc
    17701 tataaggtac gtttctatgc ttctagtata tgctccaaag gcaacaaaaa aaagaccaaa
    17761 taaaacacag gcttgtgttc aaacttggac tttaccctaa tacagatctg tttcaatatc
    17821 ttgactggat aaattggtta ataattcatt ggtaatcagt ttttgtagcc gttgtttgag
    17881 ttctcgagcg gtccttccgt ctgcgatata tcccgacaaa agcttgtcta gttctactgc
    17941 aaaaacttgt ttttcatctg ccaaactacg atattttgca aaatgagtca tgtttagcac
    18001 atcatcaaaa tctaaaatgg ggtgaacctg caccaaaggg aaatccgttt ccaacccatc
    18061 gctggttgta atcaagaaat caacctctag taagtcctcg acactcttga attgttcagt
    18121 ggtaaagacg gcgccaatct tcttatctgg caaatgatgc tggcattgtt tataaagtaa
    18181 gcgttggact gacacccctt catcacagat taaatagaca atctgtgtct gtgccttgcg
    18241 cgtcccactg cgtcgaatag ctcctcctag atgaatcgtc aaataggcaa tatctccatc
    18301 ggataactca atctgccact cctcctccaa aatctttcca gaaacccgtg tcatagcaaa
    18361 cagggtgctg tatttatctt ggatttgttg gaccattgga tttttagata ggatgccgta
    18421 ggtttttctg aacaacagag ccttacaatg tgccaagaga tgtttggcta attcgtcagg
    18481 attttccaat tcaaatggtg aatgtatctc aaaatgatga ataaatcgct ctaccactcg
    18541 cttcaagtca atgaaatctt ggctgaatac atgaacatcg ctgtcctttc gataggatag
    18601 aagtaaaatg gcaatcaggg agatttctat ttcatccaat ttcaaatcaa aggtttgcaa
    18661 caaggcatct ttcaattcct gagcaactcg gtactcgata cgctctcgaa cttgttcaaa
    18721 ttctttgaga atggctcgtt cttccaagtc atccagctcc atattgcgat agctaaggag
    18781 caagaaaggc aatactttga gcataaactc gatttcgtag cgattgattt tcttacccag
    18841 tttcttttct agctgcgata cttgtccctg taaaaattgt gccatttctt ctgagaagag
    18901 caactgacac cccaccaact caaataactt atcttctaga acctgcaaaa aacctttatt
    18961 agcatccaag aagattgtat agagaagtga gtggacatat tggattttat ttaaagggtg
    19021 acaaacgaga ctatagccat gactcttaga cacatagagg gtcatcttgt attgttcttg
    19081 agccaactgg gttcgaattt cattcaaatc atttaagacc gtattacgag agacttctgt
    19141 caactccatt agacgttcaa tggtcactct ttctggtgtg atacagatat ataccaacat
    19201 cagctgaatg cgctcaggca tgctcatgac gtaggtgtac gaatcaatgg aatcgagaag
    19261 atctcgacag gctagttttt gcgcttcggt aagtcgaata ccgaggcgag gtaagctttc
    19321 aatactctct acagttgttg gtagggcatc gtttattttt tctagatggt aatagatctt
    19381 tcgacgtgat tgttgcaagc ccttggaaat agccatgatt gtctcaggtt cctgcagacc
    19441 aatcaggtac ctcagtagag cgcaactact cttatctaat aacaagattc tcctcctctt
    19501 tcccccgtcc cgttactatc ttaacataat tatctgggat gttctaataa ttattaaaat
    19561 tcatctgatc attacttgct ttatgttgca taaatatttg tagttagaat taatagatgc
    19621 ttttaataat ttctacccgg gtaatcagtt ctccgtgcat gttatctatc cgaagggcaa
    19681 catttttcaa atatacacag gcaccaatag caatggtttt ctcttcttca ttgagttttc
    19741 gtgtcacaaa atctctcaaa gtctcgctac cttcacttgg aatttttact cgtgcgatga
    19801 tattagccac ttttactttc tggctggcgc gcatgataat attattggaa tcatcaaact
    19861 gagtgaagag gtattttcta actgcaaata ggatggcgac cgaaataatt ccaatcaggt
    19921 aatcactact agaaccatgc tctacaatca gttggcgagc aatagcaacc aggagtacct
    19981 caatcacggt gctaggtgac tgtttgtaaa gcattttgat aaattcgact ccgactgcca
    20041 aggtcattgc acgactcaga aaattttgaa taaacgtatc cgcttgaata tcaccagcta
    20101 atgagctgag taggtctgca aaaagaagcg ttgacaaggc taaaagtgct aagccaagta
    20161 tggcagataa aatcaattct aaatagtagg ctgtctctgt cagactatcc tgtaaaaatt
    20221 tcctcatctt tctcctcatc aaaagggaaa aagcagttct cctttttggg gagtctgcta
    20281 cttttccctg ctgttttttt cacacaagaa cttaatgttc atcacccttt tgtccatagt
    20341 aggcattggg accatgttta cggaggtagt gcttgtccat gatatagctt ggcgctggct
    20401 ccacccgtgg attgatttgt tcggtatagc ggttcatccg agccacttcc tcaaggacaa
    20461 cgctatggta aactgcctga gcgggatcct tgccccaggt aaatggtccg tggttacgga
    20521 caacaatacc cggaacggcc actggatcaa tacctcggcg ttcaaattcc tcgatgatga
    20581 ctgctcctgt ttctttttca taggccgagt tcacttcctc agccgtcaaa gaacgagcac
    20641 atggtaccgg accatagaaa tagtccgcat gggtcgtccc ataaaatgga atgtcacgac
    20701 ctgcctgagc ccaacccact gcttctgtcg aatgtgtatg cacaacggca tggacatttg
    20761 gccaagcctt gtagagggca acgtgggtcg ccaaatcaga cgaaggattg aggtcacctt
    20821 ctaccacatt tccgtccaag tccgtaatga ccatattttc tggtgacaat ttctcatagt
    20881 ccacaccaga cggcttgatg acgatgcgac caagttcacg gcagacttct gatacgttgc
    20941 cccaggtaaa cttgacgagc ccatgagcag gcagggcgat attggcttca tagacccgtt
    21001 gacgcatttc ctgtaaatct tttggcatta gttcaacccc gctttctcaa tcaatggata
    21061 gaggaaatct tgtgcctcct tgatagctgc tcgtgtttcc tcaaccgttt cacaattctc
    21121 tgaccacatt tcaatcaaga atggaccttg gtagttggtc tttttcaata ctgcaaacat
    21181 attgtcccag tccacacagc cctgaccgaa tgggacatca cggaattgac ctttggaatt
    21241 ttcagttacc gcataggtat ccttgaggtg aagggctgcg atagaacggt gtcccagata
    21301 aaattcgcta taaatgtcat tatgccaagc agacacgttt cccgtatctg gatagacaaa
    21361 gagataaggc gagtcgattt ctttttcaac agctaggtat ttttcaatgg agttgataaa
    21421 tggatcatcc ataatttcaa tggccaacat gacctgagcc tgttctgccc agtcgcaagc
    21481 ctgacggaga ttcttgatga aacgctctct ggtttcaggt gatttttcct cgtagtaaac
    21541 atcatagcca gccaactgga tgactcgaac gcccaaatcc tgagccagtt cgatacactg
    21601 tttcatggtt tctaaggact tggcttcaag tgcaggggca ttggagccaa gcgggtagcg
    21661 acggtgacct gagaaacaaa tggtcggaat gcggacacct gtttcataga tggccttgac
    21721 caagtctaaa cgctcttcct tggtccactc caaacgagcc aaacgagcgt cgctctcatc
    21781 caccgacatt tccacaaagt cgaaacctaa ttctttggca aactccaagc gttccttcca
    21841 tgtgaaatgc ttgggcgttg ccttttcata tattccgatt ggtcgtgcca taggccttac
    21901 ccccagatac gacggatttc gtctttaaat tcacgcgccg cagctgctgg atctgctgct
    21961 tctgtgatac cgcgacctgc gataaaggtg aagacatcta caccctcaaa gagtttgagg
    22021 gtatccacat caagaccacc tgttacagaa acacggaagc ccatgtcaac caattttttc
    22081 actttattga ggtctttttc accccaggtt tcgccagcaa ggagggcgtc acgcgattgg
    22141 tggtaaatcg cttgggaaat acctgcgtcc aaccaaagct gagcttgttc aaacgtccaa
    22201 tcaccgtaaa gttcgatttg gatttcaccc ttgtcgccac gcacttcctt gatagccttc
    22261 aaagcagctg ccatagttgg aatagttgct gaacaaatac aagtcatcca gtccgcacca
    22321 cgaacagcat tgtttttagc cacagtacca cccgcatccg cacacttggt atctgcaacg
    22381 ataattttgt ctgggaagag gctacgaaga acttctacca attcgctacc aacttgaagc
    22441 aaacaaacgg tacctgcctc gataacatcc acttcatgac caacggagac agctgcttta
    22501 atcgctccct gcaagtctga atggtcaagg gcgacttgta aatttggaat atgtttcatg
    22561 ttttttctcc taactttcta aatctaaacc ttctaggtaa ggactggttt tggactcttc
    22621 caccattgcc aagacttcct caggagtttg acaagcaatc aaacgctcaa tcgaattttc
    22681 caattcaaag agggcaatga tttgtggaat agctacggat gtatgaatct ttggatcggt
    22741 agctgcaaga gtgagcagaa cagacacttc tttcccatct gagaagacga ctggttttgt
    22801 caaggtaatc aaagcaaagg aatccttcaa gacgccgaca cctgcttggg cgtgaggcat
    22861 agccatacca ggcatgagca catagtaagg tccatattct tcagttgatg cgatgatcgc
    22921 gtcgtagtat tcttctgtcg cagcaccgct gtcaatcaag ggctcaacag ccttgtgaac
    22981 agcctcctgc caggtttccg ctgtcaatcc caagcgaatg gaattatttt ctgtaaatga
    23041 tttttgtaaa ttcatagttt ttcccttcta cacgctatat gaaaggggga gggaaactcc
    23101 ctacccctaa cggctattta tagcactgct tgtaattttg ttttgatttc gttgtcgtcc
    23161 atgaggttgt caagaccaac caaatgacct ttcgtgcgac cttccaactc agaaatcaag
    23221 tggttagagg ctacgacaat atcatatcct gccgctaaac cttttgcttc accgactgag
    23281 caagaagctg attgaaaatc tgttacgccg agttgacgaa gggcgttttc taccttcatc
    23341 ttgatgacca tagatgagcc catgccattt ccgcaggctg ttaaaacttt aaccataatt
    23401 tcactcctat tttattttta atacattatg ctctgatttt acagaccaag gatactattc
    23461 tgcttgtgcc tcaccacgat agtatgcttc cttatctttt gctttcgcaa attgaagttg
    23521 cggaatagcc aataagaaga ggcagacaag aacataacca gcaataccta ggtacttaaa
    23581 tgcaaatcca aatggtaacc atggcacttc aaagtcaatg tttccgtggt aaccaccgct
    23641 caattcaagc aagccaacgc agactgcacc aagagcaact tgaaggacac ctgagatgaa
    23701 ggaaaggatg gctgccgctt tccaaccacc acgtttgtca gcgtacactg cgatagctgc
    23761 gttatcgaag aatacaggta cgaaaccagt gatgatgagg attggactct tgaagacaat
    23821 caagaggacg attgtaatca actgaccgat taaaccgaag gcaaaacctg aaagaactgc
    23881 gttagatgaa ccgaagccgt aagaagctgc aacgtccacc gctgggaatg aaccaggaag
    23941 caatttgctt gagatacctt ggaaggcatt tgtcaattct gctacgaaca tgcggacacc
    24001 ttgcatcaaa atgaaaagat atactgagaa ggtgaaggct gtttgaatca catacataaa
    24061 gaaggcttgt ttagctgggt tgtatggagc acctgaagtg ataacttctg gatttgccat
    24121 gatttctgga cccaagatga acaaaatcac accgaagaac acaagcatca atgtcgctga
    24181 agcaacgact gtatcgtgga agatgttgag gaagctaggt agcttcaagt tatccaagtt
    24241 ttcttctttt ttaccaagtt ttggtgcaat cttatcgaca aaccagattg cgaattgttg
    24301 ttggtgaccg atggcaaatc caccgccacc tgtcaatctt tgcgttgctt caactgtcat
    24361 atttgagctg actgcccagt agataccaca gatgataccg atcgctgctg taccgaattg
    24421 gttacgaaga gccgggatga ggaagagcac catcaatgag actgttgccg cttgttgtac
    24481 catgatgtgt ccagtgatga acagagtacg tactttggta accttacgta gtgccaccaa
    24541 gaggatgttg acaccgaaac caatcaagag tgctgtagta gctgctgata cgaaattagg
    24601 gaactcctca gcaattttcc catttgctgc tgcaagacca aagtaagggt caataactgc
    24661 cgcatcaatt tggaacttat agttcaaggc cgccaaaatc ggacggaagg ttgttaccaa
    24721 accaccagcc gctacgttca aaatcatata gccgactgtc gctttgataa accctgcaaa
    24781 aacatcatga ggctttttct ttaagagggc atagcccacc aatactaaaa gacctacgaa
    24841 gaaggctgga ttctgcagga tattctgtga gaaccagttt aatggggttt gaaggaaatc
    24901 catttttcat tctccttttt tgaaatgatt tttatggttt tattataagt tatgtaagcg
    24961 gaaacaaaaa gaccaaataa agcacaggct tgtgttcact attggactat ttccgaactt
    25021 taatggctga tttaatcaga attcctccct atagttcacc agcccattta ttctaataat
    25081 caataaatag acgttatttc ccctaaaaat ttaaaagaac ctcaaaaaac ataaaagaag
    25141 aggttggttt gaaaccaacc tcttctagag ggaagtgata ctctcaactc ttgtatttat
    25201 aagacattca caagattttg agcaatgctc ctctggagag ccaaatatta ttactcttct
    25261 gattttttag ttcttccttg cgcagcaact gtcaaaccaa gtccagcaag tccgagacct
    25321 agaagtgcca aatcgctttg tcggtcaccc gtaatcggca gactcgcctt gcttgcacca
    25381 cttgtcgctt gtccagatgc ttgagcagta gtagaaagac tcggtttttc agcctctttc
    25441 ttagcctcca tactgccttt cacaacaata cgtgtttcgc tatcaacaac tgtttctgat
    25501 aacaaattag tagcaactac cttaccatca atcaagacat cctgataaac cagaactttc
    25561 ttaccagttt tgccttcttg aaggacataa gattgatcgg tagagagact tgcatcatag
    25621 cgaatttcct ctacgatagg aagaattttt tcctctgtac gttccttggt agtaaagtct
    25681 agacttggtt tttcttcagc agtaggcgct gtatctggaa ctgccgtagt gatgtaaggc
    25741 aagcgaatca ccgttgcagt caacggtgca agcacaactg catcatctgt caatgtcaca
    25801 cctaccaaat cagtcagcgc tgctattcca gcccgcttac catcgaccag cacgtgaccc
    25861 ttaagcaatt ctttgtaaat atcagaaaga acaaagctgc gctccttact gtcggcgtta
    25921 acaaagacag catagcggtc cccattgctt gcaatggttt gataggcaat gaccacatca
    25981 tttttggcaa tgccattttg acctggaatt gaaatcaagc taaccttctg ttcaacttct
    26041 tcttttgttc ccaaacggaa ggcatctgta gatttgcgca aggcaatcaa tccttttgta
    26101 aaggcctgag tacgggtatt ctctggatag agcgcctcat ctgtcgcttt ggtccagtca
    26161 aacttgttga ccgcatccga tgaatcgtaa gaatcatgaa tgtagtatgg atagtcaaat
    26221 ggcgtaccat ctgcatttgt caacaaatga gctttgttcg gtaccttgtc ctctgaaaca
    26281 ggttccttgt aatcaggatg gcggaactgt ttggtccgtc catactcctg accagaatga
    26341 ataaacggtg tcccttgagc agtcaagact agcaaattac ccaaacgttg acgttggtga
    26401 atttcagtgt aattttcagc aattgatgga tccttcttga tggactgagc gataatatca
    26461 aagagggtca agttatcatg cgcagcaata tactggatca catcacctgg atcatctgcc
    26521 aagaagttgc ctggttgtgc cttgatgtta ttgaagacag attctacact cttagcaccg
    26581 cctgtgataa aggctggctg accttcgttt ggataaccag atttaagtgt attgcgaatt
    26641 tcatctgaga ataccgcaac cgtatctgtt gaactcatcc agtcttggtc cgctggctga
    26701 acaggctgat tggcatcacc tgtaaaggtt ctccatcctt caccaagcat aatgatgttt
    26761 ggattgagtt tttgtgcctc ggtaaaggcc agctcaattg cttcggcatc atggtcaccc
    26821 atcatatcaa agcggaaacc atctacttta aactcgtcca caagatactt gatagaatca
    26881 acgaggacac ggcgactcat ataatgagtt gttccgagac gaccaccacc aaagcttgat
    26941 ttagccgtac catctgcctc catgaagtga taatagtttg gctctaagtc ttcaaataag
    27001 aaggtcttag aagtgtggtt atagaccaca tccaaaatca cacccatgcc ttgtttgtgg
    27061 atttcattga ccaggttttt gaattcctcg atgcgtttca ttggatcggt cggatctgta
    27121 gaatacatac ctgtaaaggc aaagtagctc tgtggatcat agccccagtt gtagttgctg
    27181 ttgctagaag catactcact catccgctct gcatttttca attcattgac aaaatagtag
    27241 ctcataactg gtagcaactg aatatgggtc acacccaagt ctttgaggta gctgagacgc
    27301 tctgcaaagg ccgcaaaggt accgaactga gctgtcaatt catcagaaat agcaggatcg
    27361 gatgtgaagt cacggacgtg agcctcatag ataatagcat cttcacgctg attgaagtta
    27421 ggaattgtgg catagttcaa atcacttgga ccaacttctg ctgggtccac aatcgcagct
    27481 tttgcaatcc gataagatgg atctgtatct gccaaatcac tattccactc cgctaaagat
    27541 ttagcatatg ggtctaaaac aagaacagat tgacccccgc gagtgatttc ataatggtag
    27601 tagtaaccac gatagtccgc gataccaaga cctgattgac tgttcaattc gaggtcccat
    27661 tgtcccttat cccccttggt catggccaat ttaccgacca ccttagattg gtcatcctta
    27721 tcataaagaa cgactgccac actgtctgca cttggagacc atagggtcat atcaacgaca
    27781 ctaccagcct gacgcacacg cgcaccaagc tctccatcat aagcatagag ctcatccttc
    27841 aactgccagc tcatagttgt tgtgaagcta tcattgccat atttgagggt atagcccgca
    27901 ttttcttggt tgaaatcccc gaggactcgc accttgttgc tggtaaggtc caagacaaga
    27961 tccgtcacag caactgtctg accattctta tctgttacag acaatttctc caagatgcta
    28021 tccttatccg caccttccaa tgttgtaaag gctgcttcta aggctgttag gctaacgtga
    28081 gaaacacctg tcacacggac attattgaca aaataagggt tggtgtaaat agtcgcatca
    28141 tcatccttga ggaagatttg tgtctgattt ttcaaatctt taaagctata atctttctgc
    28201 tgaatcttca catcatctcc tgacttgctt tcatccaata gcaagaagcc aatggaactt
    28261 ggtaaatctg tcaatttgac atcgatatag gcaccgtact tgccttggtt ttcaaaatca
    28321 ataccatttg gccagtcacc caaggtaagg tcagtcacat caccccaaat ccagagacct
    28381 ttttggtcat aattgccatc ggtacggaag taattgatac ggatgcagcc ttcttttaga
    28441 ggttgataga ggctgagttg gtagttttca tcaaaccaag cttcattcat cttggtgcta
    28501 atccgttcaa tggtcttgtc acctgtgatg ttatcaccag aagtgttatt aatcaacaga
    28561 ctaatcttgc tggctgtttc atctttcatc ttgatgtcca gataatagcc ataatcatcc
    28621 tgcttagcag tactgaaatt ggttgcacct gttggccaag caccaacatc tgacgatggt
    28681 ttttcgacat catcccatgt ccataaacct aggctagata aattatcaga tggtagagtt
    28741 ttaaagtgaa cacggaagta acctgcttca attggttctt cagttgctcc ttgcacactt
    28801 gcattgacga tgtcacctgc ttctgtctga aggagcatgg tgccaccttg accgattgtt
    28861 ggaagttcga cttgaacaac ccatttacca cctttctcga tagcttgata agattttcct
    28921 gtgtaggcat ctgtgacaac tacatcttta ctagatactt caagcgccat ttccttgctc
    28981 tcggtattgg ttgagaagac aagataggct gcttgatctt ggtaactgcg tttgctgata
    29041 agccaacctt ggctatcatt tccagcaagg gtgcttgtat caccacgtga gagcaatgtt
    29101 gaattggcat tgcggaaagc caataatttc tgatagtggt ctgtgatgtc gctagtctct
    29161 accttgctcc aatcgaaatc gtaccggttg tcataaactg gccagttgtt ttgcccagat
    29221 tgccccactt cttcaccgta gtaaatgact ggctgacctt tagctgtaat caaaagacta
    29281 gcagccaatt tcagtttgtc cagatttcct tctttttctg cgccaccgag actgtagagg
    29341 aaaccatctt cgtcgtgact gcccaagaat tgacccagag aagctgcgct agtcaaaacc
    29401 ttgctacgct cttctagctc ttttccagct gctttcagtt gaccattgac caagtactta
    29461 gcgatatcct tgaagccaaa gtccaacaag ctgtccatag taccgattcc caagtcaccc
    29521 ttggtatctt tatagttagc tccccatgtt tctccaatta agtggaagtc aggatctcta
    29581 tctaccaatt catttttgaa atgttgccat gtggtatcat caacgtgttt gactgtatcg
    29641 acacggaaag catagataga gttgcccttg gcagttgttg agcgttccag ccaagatgcc
    29701 tgccaagcaa ccaactgatt gcgaacagca gcttcttctg tcttgaagtc cggtaatcca
    29761 gataatgagc ctaattggtc atctccctgc ttgtcttctt ccttagtccg taccatacct
    29821 gcaaaggttt cttctgtacc atagccagca tggttcagca ccacgtcaac aacaatattt
    29881 ataccttttt ccgccgcagc atcaatcaat tcgtggaaat cggctaaact tcccaaatgc
    29941 ggatttagtt tctcaaaatt ggatgcccaa taaccatggt aagcatagta ctctccatct
    30001 tttacgctac caacattctg tggaatattt tccacaatcg gagtcaacca gatagaatcc
    30061 acacccaatt ctttcagata gtccagcttg gctgtcactc ctttgaagtc accgccctgg
    30121 taaagaccgc gtgggttggt gaccgttcct tctaccagct tatcattgct ggtgtctcca
    30181 ttatagaaac ggtctgtcat catgaagtag atgacttgct cgtcccagtc tttttcacca
    30241 tctgccttga cacgctctgt aatggtcaca tccaattttg tttcgtagta accattgtct
    30301 ttatcataga cttttacagg aagactatat gtacctggcg ctgtgctaga cgtcgcttta
    30361 atggtcacac gattgagctc agtagagatt ggcacaagac ctccgccgat ggcacttgta
    30421 tcaacttcca ttttgactat ttcagcattt tcagggttgg tgacagttac agataccaag
    30481 ccgctttcat tgtagttgaa tggtgctttc acacttgcct caacagtcac attttttgta
    30541 cccggagtag ctttgcttgt atctaattct actttttctt cgataaagta tggatttgtg
    30601 taaactttgt cgtcaccgtt tcgaaggaaa atttggctat gccgtttacg gtctgcaaag
    30661 gctaaatcaa tggttttatt gcctgccaaa tctggattat tctgattgat tagcagaaat
    30721 ccaagcttgg aatctaaacc attcgagagg ggaatgtcaa cataagcgcc atatttacca
    30781 agtttaaagt caacggcacc tgttggccag ccatcactcg cttcagtcgt atcgccccaa
    30841 gtccaaacgc cccaaccttc ataattattg tcttcacggg tgtagttgat acggaggaca
    30901 ttttcatctt tgagcggctc atagctagac acgctataat cactagagat ccaagcctca
    30961 ttttgttccg gaacaagaag ctccactttt tggtccgatt ctgttatttt ttccccattc
    31021 ttgagaacca gataaccaat cgttcctggt gtctcagatt ttttaatatc aacgtaatgg
    31081 ccgtagtcat ctacctgact gcttgaaaaa ttcgctgtat ccgttggcca ttgattacta
    31141 tcggacggct ctgccactcc tccccaagtc caaagtcctt gactttctgg tgctgtttca
    31201 tctacatttt caaagtgcag acgaatgctg ccttcttcga taggagcttc ctctggagca
    31261 gttgcatcgg ctgtttctgt taccgctgtc gcttcagcta ccatagaatc tgtagcttct
    31321 atcgcagttg tggtcgcttc taccgcgccg ccatcctgtt ccgaaagagt cgttgaacta
    31381 gttgacgcag ggaggacaac ttcctctgcc tgcactagtt gacctgctcc acttaaaaac
    31441 agagcagccg caatcccaac agaggctaca cctacattta gtttacgaat gccaaaaatc
    31501 tgtttcttct ctagcatccc tgttcgtttc atattctgaa cggatttctt tttcatagac
    31561 tcctccatat aaataagttt gaattgtaca ctaccagtat atacaattcc aaggattctt
    31621 tgcaaacgtt ttcttaagtg ttagcggtaa caagttttgg gccactatct aacaaaaata
    31681 gaataaatag ctcccccttc ttttgctcct agaccgccca ctgaccaaaa aagagactac
    31741 tgaggtgtag tctccctttc aattaaggtt gcctggcagt aatgcccctg tctagccaga
    31801 ggcttgccat ccaatatcgt cattaaatgt ttgacgctaa gagcccccat ttgacgaatc
    31861 ggctgcttca tcgttgtcaa ggctggtctg gacatccgat ctaaaaagaa gccgtcaaaa
    31921 ccaatcaccc caacgtcatt cggaatagac tttcctagag cttgtaaacc tcttgtaacc
    31981 ccaagagcga gacgatccga tgcgcagata aaacaggtgt tttctggtaa ataatcaaga
    32041 tttttcacaa attcttccgc aacacgagaa cgattttcca atcgatagag atgagtttct
    32101 cggccctgca tcgcatcttt gtagccttct tctctccctt tttcaaaggc ttctggcaag
    32161 tcaattccta caaaaacaat cctctcataa ccttttgata aagcaaactg ggttgcggac
    32221 ctagtcgcca aacgattgtc tgaatctaca aaaggaaggc cttcttgatt ctccccgaat
    32281 aaaatcaatg gcttccgcag ccctttcaac caagggtagt cattcttgcg agcaccagtc
    32341 acaatgtaac catcacattg atcagtaatc tgacttggat tggtcaccaa ctgcaaggta
    32401 taatttcgat gattgagctc atcagcgatt cctgcaagaa ggtcgatata gtacggctct
    32461 accacatcca tcttttcgag aatgacagcc tgaacaacca gtgtccgttg ctgggccaag
    32521 gccttggccg ccacatttgg cttatagttt aattcttcca tcgcagctgt tacgagcgtc
    32581 cgcagctctt gtgtaacttg ttctggatgg ttgatgacac gcgaaacagt catctttgat
    32641 acattggcta acctagccac atcggctaat gtcgtcatag aactcctccc taattctatt
    32701 tctgttgctt atcttttagc aagcaaacag tcaaaattgt gcaaacgttt tctttaggtt
    32761 attatatact atttattact aaaaagtaaa gacctttcga atacatttat cgtcatttag
    32821 ttttccttct attacttcct tagcttcctt gcctaacttc atctatgcct gcggctcatc
    32881 gcctcgtgct aaaagtacta tataagaata tccattccct ctaaacaaaa aatactccaa
    32941 ccgctagacc aatcaatgtc taacaattga agtatctttg tcaaattctg tccattttct
    33001 gtacgattat ggaaataaat aagataaatt aatttgctta gcttactcaa catctacctt
    33061 ttgaatgcga gctaagaatt gcttagtacg ttcttcttgt ggacggtcaa aaatgtcttc
    33121 tggtcgacct tcttcgataa tccgaccacc atccataaat atgactcgat cagctaccgc
    33181 acgcgcaaag cccatttcat gagtcacaac caccatggtc ataccttctt ttgccaactg
    33241 catcatgaca gccaagacct caccgattaa ctcaggatcc aaggccgagg ttggttcatc
    33301 aaaatagatg acctctggat tggtcgcaat ggctctggca atgccgaccc gttgctgctg
    33361 acctcctgat agctggcttg gatagaaacc atatttacca gatagtccca ccttgtccaa
    33421 ggctgcttta ccgattcttt cagcctcttg tttgggtact ttccgagcca caatcagacc
    33481 ttccgtcaca ttttgtaagg cagtcttgtt ggcaaagaga ttatagtttt gaaaaacgaa
    33541 ggctgtgcgc ttgcgaatgc ctgcaatttc cttcttggtc atctgagcca agtcatagct
    33601 gtcctcacca aagactaagt gacccgcgtc agctttttcc aaaaagttta aagaacgaag
    33661 aagagtcgtt tttcccgatc cagacgggcc taaaatgacg accacctcac ctggattgac
    33721 ctctacatca attcctttta acacctgctg accaccgaag gtcagcttta ctccacttgc
    33781 ttgaatcatc tgaacctcct agtaaacggc taacttcttc tcaccatagg cttgaagctt
    33841 ggtcaaaatg gttgaaaaga gtagataaac aagggccacc tcacaataga gggcaaacgg
    33901 ttcgtaggtc cttgctgcaa tctgctgagt tgccataaac atctccaaaa cggtaatatt
    33961 ggcagccagc gatgtatcct tgaccaatcc aatcaaggtg ttggacaagg gcgggaaggc
    34021 cactcgaaag gcctgtggga gaataatccg acgcatggtc tgggcaaaac tcatcccgac
    34081 agaatagccg gcctccaact gccccttagg cacggactcg atagatgccc gaatggtttc
    34141 cgcagcgtag gcacctgtat tgacagaaaa aaccaaaacc gccgacggaa aggcatccaa
    34201 gacaattcct agacttggta gcccaaagaa tatcacatag agttgaacca agagtggtgt
    34261 ccctcgaaca acccaaatat agaaacgagc cacttgtttg agaatgggaa tctgagcaat
    34321 ttgcaccaag gccgtcccaa tagcaattag caagccaaag gtaaaagaca ggattgtcag
    34381 tgggatagtc accaacaatc ctggaacaag tatctgtgaa aatgaatcaa gcaatagttg
    34441 ccatactctt tctgtcattg tttctcctga ttacttagct tttgatacgt cttcaccaaa
    34501 gtatttgttt gaaatttcaa ctaattttcc ttcactagcc aattctgcca aggcggcatc
    34561 aatctctttc gcaagatctt catttccttt tgcaactggg aaggctgttg tcgatacgtc
    34621 atccgtcaag gtcacaacct taatcggtgc atcagcatgc tgtttcacat aatccaagta
    34681 caccaagttg tcattgatgg tagcatccac acgccctgta tttaacaatt caactgcctg
    34741 agagaatcca tccacaccga caacttctgc accattttca cgcgccaaat ctgcccagtt
    34801 acttgtcaag ctgttggctg attttttacc agataaatct gcaaagcttg taatgtcagt
    34861 attatccttc tgtgttacca aggctccata gatgtatgta tatggtgttg aaaaatcgta
    34921 ttttgcctta cgctcatctg taataccgac ttggttagcg atggtatcaa aacgggccgc
    34981 atccaaacct gcaatcatgg aatcccattc tgtttcaaca aattccacct taacacccaa
    35041 tttctcagca acggcttcgg ctacttctac atcataaccg accaatttcc cgctctcatc
    35101 gtggtaagag taaggagcat aggcaccttc agtaccgatt tgaataacgc cttcttcctt
    35161 aatttgtgcc aagagggttt ggtctgtact attcgatata cctgcagatg aattgcaggc
    35221 tgctaacacg atacccgcaa acaataaact tgtaaacata gctattttct tcataattaa
    35281 aacacacttt ctttttcaga ttttacaata gtatcatgaa taacaatttc cagccaatat
    35341 attttttcta tcagagacat taaaaatttt aatcaagtat cggagcataa aaaaagacct
    35401 actcggtctt tatatctcat attaattata ctccagctgg tgggtaaata tagctgactg
    35461 ttccttcgct agtcgttgtt ggatcaaaga aacctcggac attacttggg tagtatactc
    35521 caccatagtt tgattcctta acttgaatgt gaccgttagc tgccacatct gttacatagg
    35581 caacatgacc gtagcctcca tctgtccata cagcaatagc gccaactttt ggagtagttc
    35641 ccacttcata tccaagcgct gccgcactag ctgcccaatc tccaccatta ccccaatagt
    35701 ttccaaccca aggagccatt tctttggcac cccaagtaca ctgaccagct ggataggtat
    35761 tagccgtata ttccacagca acctctgaca ccatattggt agccaaagca gcctttgcag
    35821 cggcagcagc tttctcagcc acaatttttt ctgcagcagc tttggctgct atttttgcag
    35881 cttcaacctt ttttgttaac gctgatactg tcggactatc gcttataact gtgtcatctg
    35941 cactgacatt caccattccc aaccataaaa gatttcctat gagggctgta aacagtccca
    36001 atttgaattg tttttttatc atttgataac tcataatatt tgattccttc gtgttttttc
    36061 tgccctatct atcctaaggg ataatccttg cagagagctt aaagaaatgt aacaaatatt
    36121 acagataact tgcaatctct taacaatcac agtgttcgga ctttttgtca aaaaaacgac
    36181 ctccaatacg gaagtcgttt tctatttagt gttagtgtaa ttattttctc ctactcctga
    36241 gtacactgat tagaataaaa accaaaccag atataatgtt tagatattta ttcccataaa
    36301 gcggactaat cgcgataagg aaaaatccta gcgccaataa gaaaggagat ttgttcttgg
    36361 aaaacattat tcctcaccag ccttaacctt attggctgca ataacaaatg gtgtccaaat
    36421 caggaaggca acaaagaggt tgaatagtga aaccaacgca gccataatgt taccacctgt
    36481 tgctagataa gcgtagagtc ctggaggggt aatccatgga acaggagcaa agactggcgg
    36541 aattatacct gccgctgtcg ctaagtatgc gatagttgca caaatcggtg gaacaattag
    36601 ccatggaata gcaaacagtg gattcaatac cattggtata ccaaaggtaa ctggttcgtt
    36661 aatattgaat acacccattg gagcagataa tttagctacc attttgtatt cttctcgttt
    36721 tgagaaaatg aaaatagcaa taatcaaagc tagagtgacg cctgaaccac ccatttgagc
    36781 ataagcatca aaggaaccac gagtccaaag ccacggtaaa ttcgcagtac tatgagttgc
    36841 agtataagca gccgcatttt catttagtgc aaccatatag actccgtcca taattggcgc
    36901 caagacattg tgaccatgca ggccgaagaa ccaaaaaagt tgtacaagga aagttaaaag
    36961 aatgactgac ccaagacctt gtgacagacc aagtaatggc agttgaatat aagttgatac
    37021 taaatctccc aaagattgtc ctgttagagt aaatgcaaga tatgccacaa tggatgacgc
    37081 ataaattgca gcagtacctg ggataatagc agcaaaggcc ttattaactg ccggtggaac
    37141 agaatctggc aatttaataa tgacattccg ttttacaaga cttgcataga cccatgtagc
    37201 gagaaatcca ataaacaatg ctgtaaacaa acctgttgca ccgccatatt tcaatgcgat
    37261 agctccccac tggcttgttt ccaaggctga aactccatct gtcgtcacaa tggataatcc
    37321 caaaccttcc aaagcagaaa caactgactt atcaactccg tctaaagtag tggaaatcgt
    37381 tacactttga ggaatagtag agatgaatga tgcgaaggaa atcaaaccac ctgctaaagg
    37441 attcacctta tacatgagtg ataaattgta accaaaggta aaggcaaata caagtgccat
    37501 aatcccgatg gttccaaaat aaacgtagcc atttatatct atcactggtt gcatggcatc
    37561 agcaaaacca gtccatccca tattatttgg gatgtctctc agaaagacgt tcaatagaac
    37621 ggctactgta cctgccatcg taatgggcat catagaaata aaggaatcac gaatcgcaat
    37681 caaatgccgt tgcgaaccaa ttttcccaga tatttccatg aatttctcag aaatatttga
    37741 catattctgt ctccttttct attttttgtt ttatatgtcc taaatgttaa actgataccg
    37801 gctcaactga gcaacgaatc agcgcctcta actgggcatt tgtctgacgt tttatacttt
    37861 ttgaaaagat tttttccatg agaaattgat tgccttttac aaaaatatca ccagcctcca
    37921 tctcataaga atattctata ttggtttgat cattccctaa atcctctaac tgatagcgaa
    37981 catgctcttt tcctcgatta gaatggaaac aaactgcata gcaataagga gctgtaaact
    38041 cttctacagt taccttgaca ctatgttgtc ccttatcacc aaatgtttta gtataagtaa
    38101 gtccctggat aatgtcttga tcttgcaaga tggaacccgt attttgctga aaatcatctt
    38161 ttaaggattg gcgaatagta gcgaaaagtc gctcaattgg aacttgacct atttttgata
    38221 tcttcataac ccagttctcc tataattatc caatctgttt ataaagatct acgaactctt
    38281 tcgctaaatc aataaatgtc aaagcattca tcaagtgatc ttgtccatgt accattaaca
    38341 aagataattc aacagactct ccacttgctt cttttgctaa taattgtgtt tgcgatttat
    38401 gggcaatatt caatgaagct attgcgtctt ggagcagctg ttccgcttcg tcaatacgac
    38461 cttctttggc tgcttgaatc gcctggctag cgaagccctt tgcttcacca ccgtacatga
    38521 ttaaccccat aatcgcttca agatactctg gagaattcat actacccctc catcattttt
    38581 agggcagcct ctagcacctt agctccgttc atcactccgt aatccatcat attgatagca
    38641 tctaccttaa tagttggctc aaattttgcc ttatagtcgt tcaataggaa ttttacttgt
    38701 ggacctaaga gtaatacatc aatctctttt tgagcaactt cattatcggc ttcagatact
    38761 ggaacagccc aaatagttgc ttcaacccct gtttcttctg atgctttttg catcttagtt
    38821 acaagcatac ttgtagacat gcctgcgtta caaactaaca taatgtgttt cataaaaaac
    38881 tcctcctacc cctttctttg aatgagggga ataaaaatat caattaattc tttataattt
    38941 ggatgatgta acaaacgttc ttggagaact ctatcttgaa caaaatcaac cagacaaggt
    39001 gagatatact tcagaaatgc atttcgaccc tttgacggtg acaataggaa gacgaattga
    39061 acttgctttc catggcccca atccacagca ttcttatata tccctacaac aatttgttct
    39121 gaataactca tcggcaaggc tggatgaggg aaggctaacc aatccccaaa gacaaccgga
    39181 ctgtaagatt ctcgtaaatt cacttgttct ataaagcgat taacaaaatc aggctcaatg
    39241 gattcgttca aagtagttac tagttgcttc aaggctactt ctcgatcaca atcctcctca
    39301 agatagatta ttctatgtgg agcaaatact gcttctgcaa tttgcttagc ctgacctact
    39361 tccattagca cactttcccc ttggtcagga ataactgggg tcgtttgctg taaatgagat
    39421 cgaatagcct caatgtcctt ctctgatagg aagacgctga cattgataac cggtgtcata
    39481 aaaattagat tcgacaagct aatggatgaa ataatcaaat caacatcttt tagtcgttcc
    39541 tctgtaactt cataataact aaccgtatca agaatttcta agctggatga aaattctttc
    39601 tccaagcgat tctttaacat caatgcactt ccaattccgg tagcacagac taccaatact
    39661 ctggtcttct taaatccctg ataacgttca attgccgcca tcacatgcaa ggtaaggtaa
    39721 gcccactcat catccgaaat cgaatacccc attagttctg gtatcgctgc aaatgccgtt
    39781 tttgttaatt cgaaagcctc aaaatattgg tctttaattt cagcagttag gggatttccc
    39841 agttgaatat tattctccaa acgaatacgt aacggcatca aatgggccag aactcctttg
    39901 aatagctgct gatcctttga caactctagg cctgttaaat gtgacaaatg taaaagacta
    39961 ttctccacat tctgtctaaa tatctcttcc acctcaccat cttgatttgg attccccgtt
    40021 agttttacct taaggtgcaa cgcaatgtaa tttgcttcct ctgacggtat cgtcacttga
    40081 aacatttctt cgattcgttc aataatccgt tgtgccacaa gatactcttg agacgactga
    40141 atacgctccg gtattgggaa ccggtctaga ccagatccaa ttttcaaccg ttgtaaaaga
    40201 agagcgatat ggagcactag attctgcatg acaaaatctg ataaacgtaa tttagcatcc
    40261 cgacactcgt ctaagacgat taacattaag tctgtgaacc gtatatctgg caatagattg
    40321 gtttcgacaa ccccataaac agattgaaca tttccctcat caaaaaagta atccataata
    40381 aagtgacgga tagcaggttc atcccccttt agttcaattc ctttacgtgt cacaaggagt
    40441 tctaattgat aggggataaa tagttttcga atatccgcca aaatggaatg aagcgtagtc
    40501 tttccaatga ataactctct ttgtaaacat tcaggagact gaactggatc ctcgaacaag
    40561 agtttgttca gaatataccg ttgccgatca cccgcttctt ctagttgagt taaatcagtt
    40621 aaccgctttt ttaggagcaa ttgctcctgc caaaacattt caaactcaac tggccgttca
    40681 atctgtaaac aataaccact accttgtttt gaaaggatat gagcgccgct attttctaaa
    40741 gaagatgtca gttgatgaat atacgttctt accgttcgat cactcactga taataccttg
    40801 gctagctcga tgcttggtac gaatgtctcc tgttttgcaa gtaaagcctc taacaagcgt
    40861 acttcttttt tagataacat gctccctcct ttccgccaac agtctttgat ttcttatatg
    40921 tacattataa catgaaagcg atttctagag gtgcctactt tttccgcatc attaatgaaa
    40981 gtgctcggaa gctatgacca gtttaaactc ttgccacggt ttaataaagc gtaacaaggg
    41041 aagatagtct gggctgattc gtccgacctt atttaccttt ggactgatgg taaattcttt
    41101 gagtgcaatt tgcaattcac ccttgtagcg accatataaa tcattatcaa ttaagatatc
    41161 ccctcgtccc acaggaattg cttggtctga ccgagccggt acagatgcgt ccttatacac
    41221 cacacggggc atggttgaac gcagaacata agcagaaata tcacctctat aaacatgatc
    41281 aaacgctaca atctctcttt caacgggagt caaaccctcc tcaatatcaa ccataaggct
    41341 gattgtatct tcatttaaaa ctgtttgaat ggattgtagt tcctcctccg agataaattg
    41401 gtttgaaatc aaaatagaat caataaaacc cgttgccttc atccaagcaa tttgcaaggg
    41461 aagaagccag aatcgatgat cttctaaagt tggtagacct tcagataatg gccacggccc
    41521 ctcagaagct gtttgtgcac tgacaaatgc cgcagttctt ataccggatt gataataaaa
    41581 agatgacatc tctaagaagt gctcttggga taaacctgta taagcatgag gataaaaatt
    41641 atggcaggca ataatattat ccctgtttgc aacactagcc aaaagattgg acagcaaggt
    41701 cctatctgta ctcaaattca attcaacttt tatgttgtgt ggattatgtg tcatctcaat
    41761 gatttcttcc aaaggaagag cttcatcaag acgaatccca gccaacccca gttcagctgc
    41821 acgctccatc aatgccccat cccaaccttg cgctgcgata aaatggggac tgatatctgc
    41881 aatgacagtc atccctagtt gatttgcata gtcaactagt ttttgataca attcaaaaac
    41941 tgttcggtct ccattttcaa gttgcaacaa actcataaat aagcgccttg ctccgtattg
    42001 gtgcagaagt ttaatgtaag cttgactctc ttctagcgaa tagttttcag gataaattga
    42061 aaagcccagt tctaccatca ttccctccta gcgatgaaaa ccattagcat cgctcaattt
    42121 cttgaaccac ttgcctgatt tcttaacaat tctttcttgc gtgtctagat ccagttcaac
    42181 taatccatag cgattcttgt atgcatttaa ccaagaccag caatcaataa aggtccataa
    42241 catgtatcct ttacaattcg ccccttcttg gatagctcga tgtaattcaa ttaaatgatc
    42301 ttcgataaat tcaatccggt aatcatcttg aatgagtcca ttgtctttaa atgcgccctc
    42361 tccttcaact cccataccat tttctgttac aaaccattcg atattatggt aattttcctt
    42421 aatatttatc gcaatatcat atatcccttt ttcataaatc tcccaacctc tatgcggatt
    42481 cattttttta cctggcatat catatggggc aaagaaatgt tccggagtaa gaggctctcc
    42541 ctctttccaa ctattggttg gagcttgtac ccgaaggggt tgataataat tcacccctag
    42601 gaagtccaca cgattttcct taataatggc taattcctca tcgctgacct ctggcattag
    42661 cccatatgca tcaatcagct cgactaattc atcgggatat tctcctaaca cagacggatc
    42721 tagaaaactc tttgtttgaa acaattctgc aattcgagct gcttttatat cctctgcttg
    42781 ctcactacga gggtaggcag gagttagatt caagacaata gcgatttttt tgtgaggatt
    42841 tacctcatgg catgccttta cagctaaaga acttgccaac tgtgtatgat aagccaccgc
    42901 aactgccgct tttgcatcaa ccttacatgg ataatgataa tttcctagat aaccacactc
    42961 tactggaaca atcggctcat taaaggtaat ccaacgatcg actaagtcat caaacaattc
    43021 aaaacatgta cgtgcatatg cttcataggc tcgaacaatt tccttatttt cccaaccacc
    43081 tttttcttgt aaacaatagg gaagatcaaa gtgataaaga ttgacaaaga gttgaattcc
    43141 ctttgctctt accaaagaaa aaaccgaacg atagaaggac actgcctctt gattaatttc
    43201 tccagtacct gttggtataa gacgtgacca ctgaatggag gtccgaaata cagtgtgccc
    43261 agtttcaact aataaatcaa tatctttttc ataatgtttg taaaatgttg atgtttgact
    43321 agggccaatt ttgttatgaa atcgttcagg ctcaatacta taccaatagt cccagttgtt
    43381 ctgtcctttt ccatcacctg gctcaattcc ctcactttga ggtcctgatg tagaagaacc
    43441 ccacaaaaaa tttgttggaa attgataaaa taccttgtct ttagtcattg attgttcctt
    43501 ctctctttta tgtgctgtat agctggtgcg aattgtggca aataattctc atgtgcaagt
    43561 aacatatctt gtaatagcac ttcagcttgt ggtccgtttg aaataagcgg gttgagtgta
    43621 aaagcttgta aagccagtga ataatcacct gtcattgctg cctgaacagt gacaagctcc
    43681 atttgcttca tcagttgtaa atagccggtt acaatcggcg gagttggtgg acaagctagt
    43741 ggtgttgctc cttgcgcaga gataatcgag gtgacttcta ccacacaatt ttcaggtaga
    43801 tagggaataa ctcctctatt tacagtagat acagtcaatt cttctctact atcattatgg
    43861 atagcacaaa tgatttgaca ggcaacatca ctatagtagg cacccccacg tagttccagt
    43921 tgttttggtt tatctttcaa ttgtgggtct ttgtaaagct caaacaattc tgtctctact
    43981 tcttgaacaa ccttggctcg tacatttccc tgacgatact gctccaaagc atcctctaac
    44041 atttcttgtt ccaaaaaata ataacgatgg taatcacatg gcaacattcc aatcgtccga
    44101 agcaattgaa taggaaactc tagattggta atatttttga ctgcttctgg cgtctcctct
    44161 ctcaaatccc catacaatgt ttctaagatt acttgcgtac gattttcccc atacttatcc
    44221 caaacttcat gaaagtgaaa atgatttaac ccaatatggc gcgtacttag gtctccttct
    44281 gattggccta aaatctgagc agcaagtttc ttttgaccaa tagggatatt acacaaacca
    44341 ataactttct tccagtggag ttcattagca atggcttccg ttacgatacc agcaggattc
    44401 gtaaaattaa tcaaccaagc attcggacac aagtccttca tatccttgat aatcccttta
    44461 tatgcttcaa tggtcctaaa tgctttaaag attcctccag ccccattggt ctcttgcccc
    44521 agcatcccat aactcaaggg aatggtctca tctaagtatc ttgcaggtag ttggccaact
    44581 cggaactgag acgttacaaa atcagcctct atcaatgctt ctcttctgtc taaggtcgac
    44641 tctatcttcc agtccaggcc tgcttttgcg accattcgcc gtgctaactc aactacgatc
    44701 tcctgttttt ttgcgccttg ttcaacatca acaaaccacc attctcgaat gggtaattga
    44761 tgtgcacgaa gaatcatacc ttcaaccaat tctggagtgt aacttgagcc tccaccaatt
    44821 gtcactaatt taagagtttt caacacgctt tccctccctc atcttctttt atattcttat
    44881 tatatttcag tgaacatcta ttgtaaacgg atacacagac cctctttttc cgtatcacaa
    44941 cggaaattaa gacaacatga tcattctaga aaatacaaaa acgacctcca atacggaagt
    45001 cgtcttctta ttatttacat ttatttcaca acaatattaa caagtttagt tggctccaac
    45061 agtctgcgga tagactgttg gaggttggaa atagacaaac aaacgaggca atcgttactt
    45121 ggtaacgata ttgacaagtt tgtttggaac cgtaatcacc ttcaccactg tctgcccagc
    45181 aatttcagct tggacttttt cgtccgcaag ggcaactttt tcaagctctg ctggtgctag
    45241 gtctttggct acggtcaagc gggcttttac cttgcccttg atttggacaa cgatttccac
    45301 ttcgctttct accagcttgc tttcgtcata tgttggccat gcgacatagc tgattgactg
    45361 acctgtattt gtcagacctt gccagagttc ttctgccaag tgtggcgcaa atggagcaat
    45421 caattggaca aagcctttgg cgtattcgac atagagcttg tcttctttgt tggctgcgtt
    45481 gacaaaaatc atgagctggg cgatagccgt gttgaatttc aggtcttcaa tatgctctgt
    45541 caccgtcttc acagtttcat tgtaaacttt atcaagcgct ccgctatttt ccgccgcaag
    45601 ctccttggtc gtcaagaggc gataaacacg gtcaaggaac ttacggctgc cctcgagacc
    45661 ttcttctgac caggcgattg acgcatcaag tgggcccatg aacatctcgt aaacacggag
    45721 ggtatccgca ccgaactgct cgaccacatc gtctggattg accacgtttt taagggactt
    45781 ggacatctta gcaggtgcct gctccaactc ctcaccagtt tcgatgttga agaaagaacc
    45841 atcacgtttt tcaactttat ctgtcgcaac aagggcacca cggctgtcac ggtagctagt
    45901 tcccaaaatc ataccctggt taaatagttt ttggaaaggc tctttagtcg gcacaacgcc
    45961 aaggtcatag aggaacttgt gccagaagcg agcataaagc aagtgaagga ctgcgtgctc
    46021 ggcaccaccg atgtagatgt ccactggcaa ccaagctttg agaagatcct cgtctgccaa
    46081 cttctcatca ttgtgcgggt cgatatagcg gaggtagtac cagctagaac ccgcccattg
    46141 tggcatagtg ttggtttcac ggcgaccttt aacgccgtct tcccgcacca cttccaacca
    46201 gtctgtcaag ttagcaagag gtgactcccc tgtacctgaa ggcttgatgt ctgaggtttt
    46261 tggcaatacc agaggtaact cattttctgg tacggctgtg gaagtgccgt cttcccagtg
    46321 gatgattgga attggctcac cccagtaacg ctggcggctg aagagccagt cgcggaggcg
    46381 gtaagaaatt ttctcttgac caacaccatt ttcttccaac caagctacca ttttggcaat
    46441 agcttcttcc ttgttaagac catctaggaa atcagaattg atgtgggcac cgtcctctgt
    46501 gtaaggagct tcttctacat tgccaccttc cagaactgga atgatgtcca aaccaaactg
    46561 tttggcaaat tcccagtcac gctcatcgtg ggcaggaacg gccatgatag cacctgttcc
    46621 gtagcttgcc agtacatagt ccgcaatcca gattggaatt tctttaccgt tgacagggtt
    46681 gatggcgtag gcaccagtcc aaacacctgt cttgtctttt gccaaatctg tacgggctag
    46741 gtctgatttg agagaagctt ggcgtttgta ctctgccaca gcttctgtct gctcaggcga
    46801 tgtaatgcta tctaccaagt catgctcagg agcaagaaca gcataagtcg caccaaaaag
    46861 ggtgtcagga cgagttgtaa agactgtgaa gtccttgtct gtgtccttaa tcttgaaagt
    46921 cacattggca cccgttgact tgccgatcca attgcgttgc atatccttga tagactctgg
    46981 ccaatcaact tcttctaggt cgttgagcaa acgctctgca taggcagtga ttttcaacat
    47041 ccattggcgc attggcttgc ggacaactgg gtagccacca cgttcagacg ttccgtcagg
    47101 aaggacctct tcgttggcga tagctgttcc caattcctct acccagttaa caggcacttc
    47161 tgcctcatag gctaggcctt tttcatacaa cttggtgaaa atccactggg tccacttgta
    47221 gtagttaggg tcagtcgtat tgacctcgcg gtcccagtca taagaaaagc caagggcgtt
    47281 aatctgacgc ttgaagttag caatattttc cgccgtaaag tcagctgggt cattgcctgt
    47341 atccatagcg tattgctcag ctggcagacc aaaggcatcc caccccattg ggtgaagaac
    47401 gttgtagccc tgagcacgct tgtatcggct gagaatgtcc gtcgccgtat agccctcagg
    47461 gtgaccaacg tgcaagcccg caccagacgg ataagggaac atatctaggg cataaaagtt
    47521 tggcttagcc gcatctgttc ccgtcttaaa agtatgattt tttgcccaaa atccctgcca
    47581 tttgggctcg atttccttgt gattgtagaa gctcatatca tttctccatg tcaataaatt
    47641 atctttacta ttatatcacg ttttggggga attgggaaag acaaaaacag ccagttttca
    47701 ctagctgttt tcgcattttt accaaataat cacacggtct tcttttgcac gccacatgcc
    47761 gtcgccttct tgaacgccaa atgtttcatg gaattcatcg aagtttggca actggatgtt
    47821 ggtgcgtagg tgacctggtg catggacatc gacactagcc aacatctgca tgaactcagg
    47881 acgagccttc attcgccaga tgcgagcaaa gttagtgaag aattcctctg cggagaagtc
    47941 atcttctgac ttggctgctt ccaaggccgc tgcaatccca ccaaggtcag caatgttttc
    48001 tgacacggtc aacttaccat tgattttggc accgtaagaa tcctgaccct caaactgatc
    48061 aataacctgc tgggtacggg cctcaaaggc tgcataatct tcctctgtcc accagttatt
    48121 caagctacca tgctcatcaa aggaagcacc gtttgagtca aaggcatggg aaatctcgtg
    48181 ggcaatgacg gcaccgatac cgccgtagtt ggctgacgaa gattgctcca aggagtaaaa
    48241 cggagcctgc aaaatcgccg ctgggaagac aatcaagttc ttctgtgggt tatagtaggc
    48301 gttgaccatg tgagctggca tgccccactc cttaatgtcc acaggcttgt tccacttgct
    48361 ccaaccatgg gcgatgtcaa tcttgtttag ttcgatggca ttttccacca aggacttgct
    48421 tgggtcaaca atcttcttat aataacgctc tggcaaggct tctggatagc cgatataagg
    48481 cttgataaca ttgagtttga caatggcctt gtcacgcgtt tcctgagcca accagtctgc
    48541 cgtttccaaa cgagatttgt aaacctcaat catcttggca actttggctt ccacatcggc
    48601 cttagcttct ggtgagaatt tttctcctgc ataccaaaga ccgagggctt ggttaaagta
    48661 gccttgtgcc aagttgtagg ctgctttttc ttggttttgc gcctgcggtg taccagaaag
    48721 agcacggctg taggctcctg ctagaatgcg aatttcatcc gacaggaagg cagtataggc
    48781 gccagccgct ttcaaaatca aggtcgcctt caaaagctcc caattatccg cactgtaaat
    48841 gtccttggca gcctgccaga aacgttcctc aggcacgata atcttgtctg gcgtttgacc
    48901 taaaatcgct gtgaagaaat catccaaagg caactctggt gccaaggcag taaaatctgc
    48961 ccactcgtac gggtggtaaa gtttggcgta ctcagaacct tcttcatttg acagaacata
    49021 cttggcaatc gtggcatcta gttccaaacg cttgtccaag aggtccttga tttcctcatc
    49081 gctgaaacca aattttggca ggagggcttc ttggctctca cgccagattt tgagtaattc
    49141 tgcacccttt tcatgacctt cctcatagta ggtcgtatct gggaggataa tgcctagaga
    49201 atctgcccag aggacattgg tggtcgcgtc cataaagtca ggtgccacgc cgaaaggcat
    49261 gaggtttggc ttgcctgcca actcatattc tgccaacttg ctggtgaatt cttcgaaaga
    49321 gttgagagcc ttgtactctg caatcaaggc ctgagcaggt tctgctccca aacggtcacg
    49381 ggtttcatag tcggctactt gtttgtgaaa agccacaaag ttttgcaaaa cgctatcttc
    49441 tgggacatct tctcccgcca accaagcgtt tgtcgtgtca atcatgagct tttcaatctc
    49501 atccgccaag tctgagaaac cacctgttct tggcttatcg tctggaatca cagctgtttt
    49561 tgcccattca ccattgacgt attcgtaaaa atcatcttgt aaacgtgtca ttgattttct
    49621 cctttgtctt tcttaatgac tataataaca aaaaattctg acaatttcac ttatcagatg
    49681 accgtgacca agtatttttt agaataatac tcgaacaaac cacaagaagg ctgacaacag
    49741 cacaggtccc tgacatggtt aaatccagtt gattggccac taaaaaacct agcacaaccg
    49801 ttagaatagc gataccttgc cctaaaagca gcaattgcca aaaggatttg acccaacgta
    49861 gggcagccat acttggtgcc accaagaaaa caataacagt catcgagcca atcgcttcga
    49921 aggccagaac agtagttaag gaaactagaa tcaaaataac catttctaaa atccttgttc
    49981 gtaagccaga caaacgagca tggttgctat caaataggta cacttgtaaa cgatggtaag
    50041 cccaagcaaa aaatagaaca ataaccaacc aagccaaact cgacttgacc agactaacag
    50101 ggagtgacca ggctagaaca tccatacgat ggagcggagc aaaaagtact tccccctgca
    50161 aaaccagatc cacatccaag tgaacattgc gagcaaaaag agaaataagc aaaaccgcca
    50221 ccgcaaaaaa gaaagaaaaa actagacctg ttgcggcatc atgcgctatc tttcgactat
    50281 gcagacggtc aatggcaaga accgataata acccaaacag actggcacca actattagca
    50341 atggcgaatc caaactatga ctaataaaaa atcccagaac aatcccaagc aagacagaat
    50401 gcgatagtgc atctgccaac atcgattgat tttttacaac cagtatcgag ccgagcaagc
    50461 cacaagaact agcaataacc atcaaaatga gcaatacttc aaacacgacc attctccttt
    50521 ctgacatagg taacatacac aaatgcagac agggtcagac ctgtcagaca gacgataata
    50581 gtcggtccgg tcgataaacc agatacacta gagctaatcc aggtccctac gaaagccgac
    50641 ccaattgcaa cgattcctgc cagtaacaga ctcttatgat acgattttcc cagcatcaag
    50701 ccaaagacag ctggtgcaat caaaaaacta ctcatcaaaa tagctccgac taactttaat
    50761 cctactgcaa ttaggcaaat catcaaaaac atcgtcaact gctgcaagta ctttacacga
    50821 acaccaataa cccttgcaaa agtttgatca aacagatata gcttatagtc ttgataaaag
    50881 aaagtaaaca atgccaaagc caatagagaa ataattccga tgagtatcac atcatccagc
    50941 tgaataaagg ctgcctgtcc aaatagatag gtttgcaatc cagcttggga ggaccctgca
    51001 aaggcttcat ttccctgaat agcatttttc aagaccattc ccaaaccaaa gaaagacgca
    51061 gacaccaatg aaagggcatt gactaggctg tgaccacctt ttcgacgcaa ccaatggaca
    51121 agtacataag aaaggtagcc tgacaacact gctcctaaca gcaagtagag aggatgtcgc
    51181 gactgaaaaa tcatgaaaga gacaatcact ccaggataag aagcatgccc aagagcatcc
    51241 ccaagcaaac tctgtttagt aagaacagat atacttccga tagtgctggc tgctacagct
    51301 aggctgacgg tccctaaagc aactgtccag aaagaatact ctttgaaaac ctcaaacatg
    51361 actgcctcct ttggcatttc ctagaaacaa cccatttcct atgccgtagg tcgcctgata
    51421 attctcgctt gtcaaagtct catccatcgg accagagtca atcacatgct tatgaagcca
    51481 gacaaggtga tcaaagtagg catccagcgt cgttaagtca tgatgaatga caatggaagt
    51541 tttcccttca cattggaact ccttgagcat atccatgatc atgatttctg ttgcctgatc
    51601 aatccctgct agaggctcat ccataagata caattctgct tcctgtgcca gggctctcgc
    51661 tagaaagacc cgctgacgct gcccacctga aagctgatca atctgacgat ggcgtaaatc
    51721 cgcaattttt aaccgttcta gagcctgctc aacaatctgt ctatcagcct tagtcggtct
    51781 tcgaaacatt cccttactat gagcgaagcg tcccatcaga acaatttcaa agactgttgc
    51841 aggaaattgc caatttactt gactggattg gggaatatag gctacttttt gagcaatcaa
    51901 cctctctaga cctgcctcct gaccaagcaa gcgaacctgc cctgtgtcag gtttttccaa
    51961 ccccaaaatg accttgaata gactggactt cccagcacca tttggtccaa caatagccgt
    52021 ccgactccct ctgggaatca ccaaattgac atcttctaaa gccatggtct ggctggctgt
    52081 atatgctaaa ttgacatctt ttaattcaat gattgctgac atatttcctc ctatttttaa
    52141 aaatgatatg cctcattcat actcaatgaa aatcaaaacc agaccaacct accaagctga
    52201 gaaagtcgat aagattaaga taccgtacca acaaattgct ggcaaaccct atacatcgct
    52261 tatttcaaca catcagtaca tgaatttgat tttcgcagag caccaatgct tccttcaatc
    52321 gtatgtttat aaatctgtta aaaatggtcc aaaaacggaa agaactcgac ctagaccgag
    52381 tcctttttac tactcatttg aaatccctat tcacgcacag actactgata tataaagtcc
    52441 cgctgtgaac caagatcatt gaggtctaca agcggtattt ctagtcaagg acctcaataa
    52501 ctggtgtgat ttgctactaa cgcctatcct cccccaagcg ttaccacctc caatagaaag
    52561 agccctatac cgcttgtgct cgaaacaatt gattgtccct catattggta cctatcaact
    52621 ctatcttcgg taatatctct agtcttcatt gaatagcaag tccgttcgat ctgtactatt
    52681 tcagatattt aaccatcagt ttaacattat gtttgtacat gtcgataaag gtatcccctt
    52741 cctcgccttc tggtgccaag gaatctgaga acaactcctt accttcgcca gtaacaactt
    52801 caacctgacc acctttggct tttactgcct cttgcaattt cttcatgcgt tctggattgg
    52861 ttgtagactc tgtaaagata gccttgatat tatggtcgat gattaggtta actgtctcaa
    52921 tcatgtcact gttagccact tctgagtcgg tgctcacgcc ttgaggagca taaagagtga
    52981 agtcataact agcagcaaag taattaaagg catcatgtgg tgttaccaaa tagcgacttt
    53041 cttttggaat cacacttaat tctttttcta cccaggcatg caaatcatct agctgcgctt
    53101 ggtatttttc tgcatttttc tgaatcatct cagctttagc aggtaagagt ttttgtagtt
    53161 cctctgaagc tacagctaca gctgacttgt acaagggaat tgaaaaccag aagtgcggat
    53221 cgacaatctc ttctccatcc tcatccatcg tattcaagtc ttttgcatta aagtttttag
    53281 aaacagctac tccggtcttc tccaaagctt caaccatttt accttcaaag tgcaaaccgt
    53341 gatagagaac aaggtcagcc ttttgtaatt ttgataaatc actagatttg gctacgtaca
    53401 aatgagggtc ttctcctgct ggaatcagca aatcacgttc cacctcatca cccgccaact
    53461 gatagaccat gtcattgagg aaggaagtag tcacagccac tcttggctta ctagagcctt
    53521 ctgtggtttg cgatggacga caagctccta accctattag ggacaatagc agagcaaaac
    53581 tgaacaatat tttcttcata taggttctcc ttttattttt attaagcata cctaactata
    53641 taattagatt aatctaaaaa aatcattttg tcaactgatt tttaaatttt ttgatataat
    53701 agtttcagaa gaaagcgagt tctcatgaca ccaaacaaag aagattacct aaaatgtatt
    53761 tatgaactag gtcaattaga ccaaaaaatt accaataaac tcatcgcaga gaagatggcc
    53821 ttctccgcac cagccgtttc cgaaatgctt aaaaaaatgg tagccgaaga gctcatttct
    53881 aaggatgcca aggcaggtta tctcctcagc caaactgccc ttgaaatggt agccagcctc
    53941 tatcgcaaac accgcttgat tgaggtattc ttagttgagc aactcggcta ctctccagaa
    54001 gaagtacatg aagaagctga gattttagaa cacaccgttt cagatcactt tatcaatcgc
    54061 ctagacctgc tactggaaca gcctcaaact tgtcctcacg ggggaagcat tcctcaagca
    54121 ggacaaccgc tcatcgaacg ctaccagaca cggctgtcac agctaactga gacagggaac
    54181 taccagcttg tccgtatcca tgacttctat caactccttc agtacttgga acaacatgaa
    54241 ttagctgtcg gtgatttact aaccgtcaca gccttcgacc agtttgccca gaccatcacc
    54301 atccagtaca aggacaaaga gctcgccgtc ccaacagcca tcgctcaaca attattcatc
    54361 gaaaaaagca atcgcccagc ctaaacaagc tgagcgattt tttcttctaa aataaatagt
    54421 tacctctttt tcaaattacg gaaaatagat ctggctgcaa aaatagttgc ccacatacat
    54481 acaccaacca aagtaggaaa cctatataaa aacagcccaa tagaaaatga gggttatagg
    54541 ttaaaggctg caagacaaac atgagaatcc ctaccaaccc ccaccgcata ccccaggtca
    54601 tctttctctg gataatccac tccagccaat cttctccaac tgaacttctg gtttccagat
    54661 actcttgatt aaggtcttga aaaaattttc tcatatttct gcccacctca tcctaacttc
    54721 aaagcataaa ctaccacttt atgacaatat tatcatagca cattgttttc aaataaccaa
    54781 tactgtcgaa aaaaccagcc tactcgggct ggtcttcttg gagtcgctct cttaggcgac
    54841 gacggtataa aatctgcgat ggaatgaagt aaagaaaact tgccaaaata atccagcgtt
    54901 tgtcaattcc actaaagtaa atcactaaaa tcaaaagaaa ggcaatcgtc aaataaaaga
    54961 aatgatttgt gagcaatttt ttcatagtag cctccatacc tatttttcaa tattagtata
    55021 tcaaaataca caaatagagt acataccgtt gccaaaagtt ttacttattt ttcgtatcca
    55081 aaacaatagt caccggccca tcattgacca ggctaaccgc catatccgcc ccaaaaacgc
    55141 ccacttcaac aggcacaaag gccgacaagg cctggttaaa agcatcatag agttggctgg
    55201 ctagggcagg agctgcagct cctgtaaagg cagggcgatt acctttttgg gtgtccgaaa
    55261 agagggtaaa ttgggaaatg gataaaatct tgcccgccac atcttgaacg gacttgttca
    55321 tcttgcctgc ctcatctgaa aaaatccgca tattgacaat tttccgaaca gcatagtcca
    55381 ggtcttcctg tgtgtcatct ggccccacac cgactaggag caaaagccct tgttcaattt
    55441 gcccatggat acttccttcg atggtcacac tggcctgtga tacacgttgt agtacaattt
    55501 tcatcttatc cgttggtcct tttcactgag taaacttctg gcaccgactt aatcttgtcc
    55561 accagactgg tcaaggttgc caggttggaa ataccaaagg aaacgtggat agtcgcaaac
    55621 ttcatatcct tggttggctg ggcattgacc gaagaaatgt tcttgctggc attggtcaaa
    55681 accttgagaa catcattgag cagtcctgag cggttgagac cgtaaatgtc aatgtttgcc
    55741 atgtattcct tggtcgaatt ctcatcctcc cagtccacat caatcaagcg aacatcgtag
    55801 ttttcttgac tctttaggtt catacagtct acacggtgaa cagctacccc acgccccttg
    55861 gtgatgtagc cgacaatttc atcgccaggt actggattac aacatttggc aatccgtatc
    55921 agcaagcctg acgctccttc aatgaccacg ccaccttcgt gacggatttt cagactatct
    55981 ttgttgtcgt gtttgacttc gccaccattg accaattcat ccgcgacagc tttggcctta
    56041 gcccgctcag cttctcgacg ttctttctct gtcagacggt taaagattga tatggcagaa
    56101 acttctccaa aaccaactgc cgcaaaaagg gattcgtcgg tcttatagct ggtcttttgc
    56161 aggacctcat ccatgtggcg acggtccagg tattggttgg gaacgtagcc attttcttgg
    56221 agtaagttct gaagcatctc acgccctttt gaaacagaca attccttgtc ctgattcttg
    56281 aagaactgct tgattttatt gcgagccttg tgagttttaa ccaagttgac ccagtcacga
    56341 cttggaccga aagagttggc agaggtgatg atttccacct gatcgcctgt cttaagcttg
    56401 gtcgtcaaag gcaccatacg gccgttgacc ttggcacctg ttgctcgctc gccgaccttg
    56461 gtatgaattt cataggcgaa atcaatcggt acagaatcct ttggcaactc acgcacagca
    56521 ccatctggcg tgaaaacgta aatacgttct gtaaaaatat cgtccttgac agaatttaca
    56581 aaggtttgcg catccccagc cccttcttgg agttcaatca ggttgttaat ccagcgcaac
    56641 tctttttcgc tggcagttgc cttgccacca cgcttgtaag cccagtgagc tgccaccccg
    56701 tattccgcca cctgatgcat ttccatggtc cgaatttgga actcgattgg acctctcggt
    56761 ccgtaaaccg tggtatgaat agactggtag ccgttagctt tttggttggc aatgtagtcc
    56821 ttgaagcgac ctggcatagg tttccacaat tcatgaatat accctaacat agcatagaca
    56881 tcgcttggag tatccatgat acaacgaatg gcaatcaagt catagagttg atcaaaccgc
    56941 tttttcttat cgtgcatttt gcgatagata gagtaaatat gcttgggtct accgtaaatc
    57001 tgaccatgta gatggcgctc ttctgtataa tctcttagct tatcaacaac ttcattgacc
    57061 aactcttccc gttcacggcg cttctcattc atcatgcgcg taatcttgta gaactcaact
    57121 tcattgagat agcggaaaga catgtcttcc aattcccact tgatagaaga aatacctaga
    57181 cggtgggcaa gcggtgcata gatttccatt gtttcacgtg aaatacgttc ctgcttgtcc
    57241 ttgcgcaagt gcttaagagt ccgcatgttg tgaaggcggt ctgccagttt aaccagaatg
    57301 acccgcatgt cttgggacat ggctatcagc atgttgcggt ggttttcagc caactgctcc
    57361 tcatgagact tatactcaac cttacccagt ttggtcacac cgccgacaat atggcgaacc
    57421 tctggaccaa attcagcctc catatcatcc aaagttacat ccgtatcttc aaccacatca
    57481 tgtaagaaac cacaggcaac cgtcacagca tccagcttga gtcctgctaa aatacctgct
    57541 acctggatcg agtgtacaat atagggttcg cccgatttac gaaattgtag cttatgagct
    57601 tccgtcgcaa aatccaaggc cttctgaaca aaagcaacat cttcggctgg aaggtaggtg
    57661 gcagtcagcc caacaacctc ttctcctgta aaattgattt ccttcatttg tttctctccc
    57721 taactatttc ctattatttt accattttca ctggaaaaga aaaaggaaag cggtaattca
    57781 tccatactat accattcaaa atgacgataa acaaagaaaa ccagacaaac aattttttag
    57841 tcattgttcg ttttttgttg cttttatgaa aatttttctt tatttttctt ttgtgttttc
    57901 gtagaaaccg ttgatgtttt cgttttcaat atgtataata ttcttgagat tatatcaaat
    57961 ggaggttctc atgaaatttc gttttagtaa gtgtgcccta accctcagcc tagctttact
    58021 ggctagcacc aacaacgccc ttgtctctgc tgaagaaacc agcacagata cccctgtttt
    58081 gaagactgaa acagaaatca gtagctctgt agcaaccagc caagccgaag caacgactgg
    58141 tgaggttgcc acaccgacaa ctaccgaaac tacagccggt gaggctgtca cgccaactac
    58201 agaaaccact gaccagtctg ttacaccagt aacagaagaa gctgctcctg ttgtagaaac
    58261 cagcaatact ccagttaacg acacaacagc tagccctgtt acagcaacac gcgcagcggc
    58321 tactgagact acaactcctg tcgaaggcca gtctgttgat gttcgtatcc tcgcaacaac
    58381 tgacctccat acgaacttgg tcaactatga ctattaccag gataaacctg tagaaacact
    58441 gggtctagcc aaaacagctg ttcttatcga agaggcaaaa aaagaaaatc caaatgtcgt
    58501 attggtagat aatggagaca ccattcaagg tactcctctt ggaaattaca agtctatcgt
    58561 tgatcctatt gaagaaggtg aacaacatcc tatgtacgcc gccttagaga ctctgggatt
    58621 tgatgtcggc acacttggca accacgaatt taactacggc cttgcctacc tcgaaaaagt
    58681 aattcgtaca gccaacatgc ctctagtcaa tgctaatgtc ctcgatccga ctacaaaaga
    58741 ctttctctac accccttata ccattgtgaa aaagacattc acagacaccg agggtaaaaa
    58801 ggttacacta aatgtcggtg ttacagggat tgttccccct caaattctta actgggacaa
    58861 agcttatcta gaaggaaaag tgattgttcg tgatgcagtt gaagccgttc gcgatattat
    58921 cccaaccatg cgagaaaatg gcgcagacat cgttctggtc ctttcccact caggtatcgg
    58981 ggatgaccaa tatgaagtcg gcgaggaaaa tgtcggttac caaatcgcca gcttgtcagg
    59041 agttgatgcg gttatcacag gtcactccca cgcagaattc ccaggaactg ccgaaaaacc
    59101 aagcttctat gctaaatact ctggcgtgga tgatacaaat ggtaaaatca acggtactcc
    59161 tgtcaccatg gcgggtaaat acggtgatca cctcggcgtc atcgacctca atctggtctt
    59221 taaagatggc aaatggacaa ctacttctag caaagcggct attcgtaaaa tcgatacaaa
    59281 atcatctgta gcagatggtc gcatcatcga ccttgctaaa gaagcccata acgaaaccat
    59341 caactatgtg cgccaacaag tcggtgagac aactgctcca atcaacagct tctttgccct
    59401 tgttcaagat gatccttctg tacagattgt taacaatgca cagatttggt atgcaaaaca
    59461 acagttggcg ggtacttcag aagctaatct accaatccta tctgcggctg caccatttaa
    59521 ggctggtact cgaggcgatg cttcagccta tacagacatt cctgctggcc caatcgccat
    59581 caagaacgta gcagaccttt acctctacga caacgttgta gccatcttaa aagtcaacgg
    59641 tgcccaactc aaagaatggt tggaaatgtc cgcagggcaa ttcaaccaag ttgacccaag
    59701 ctcaacagaa ccacaaaacc tagtcaacac agatttccgc acttacaatt ttgacgttat
    59761 tgacggagta acttatcagt acgatattac gcaaccaaat aaatacgatc gtaacggtaa
    59821 ggtagtcaac gaaactgcca gcagagttcg gaatctgcaa tacaatgggc aagatgtgac
    59881 agctgatcaa gaattcatcg tggtgaccaa caactaccgt gcaaacggta ctttccctgg
    59941 ggtccgcgaa gcttctgtca attacttgct caatctggaa aaccgccaag ccatcatcaa
    60001 ttatattatc gctgagaaag ttatcaaccc tacagcagac aataactgga cctttacaga
    60061 tagcatcaag ggactcgacc tccgcttctt gacagcagat cgtgcaaaag aattggtagc
    60121 taatcaagaa gggctggttt acctccaagc ctcagctgaa aacgaaggat ttggcgaatt
    60181 caaatttgtc tacactgagc caaaagtagc gactccagat gaacagcagt cagatcaagg
    60241 taacacaggt caagatattg tactcgaatc tggtcaacga attactcttc cagcggtaaa
    60301 tccacctgct cctgctccac aacataaatt ggctagtcca cattcacaag cgagcaccaa
    60361 gacactccct gcaacaggag aagctacttc tatgcttagt ctgctcggtc tcactttgat
    60421 tggttttgtc ggcgcatgga ctaagaaaaa agaacattaa aataatcaag agcttgtttt
    60481 ttcaagctct tttttaccct catacactct ctagataaat taatcaaagc atataaaaat
    60541 agctcgaaga cgtactcgag ctatttttat ctttatatcg ctactcccaa taaatcggta
    60601 catagcgggt ttctttggga ctttttactg tataggttcc caaatctacc cagtttgatt
    60661 ggttcgccaa ccgctgttct actctaaact cctgacaaag gatcgttgtt tctaagcgaa
    60721 tgtttccata cttccttgca aaaacaaggt aagaaacgct ttctgaagca aagacgagat
    60781 tccccaaagt cgattttcta ggacgactaa actccataat tctaccttct tctccaccat
    60841 aaatcgaaga aatcagattt tcgctcataa tggtttcatg ataggttgtt tcgagagaag
    60901 tacgactatc agacgcttga aaacctcgac tagacgtact cgatagttga cgaataaaag
    60961 cctttctcac ttcatgattt tcaaactcat aggtctgctc tgttgcttga ttatacaaaa
    61021 ccaattccga gataatctga tagctagtcc ctggaactct accgaattga ttaagagcaa
    61081 caaagactat caagaaaaat ccaagagcag caaaaatagg ggtactcaat cttttcatgg
    61141 gcaatctcca tcacatatct cctcaatctt cgctaattgc aacgaccatt gagctatgaa
    61201 cgaatccatc acgatcctgt ttattcgaat cgttcagagc gatacattca ctatttgcta
    61261 acgtgtgttc gagactattt tctctcttta gatgataaat cattcacttt attacaataa
    61321 tagtatatca atatgatttt taaaaaacaa ctaatcaggt atgaaaatgt aaaccagatc
    61381 atgtaaaata tgaccaaata actgcaatca cgatggctgg caacatattg gccacacgaa
    61441 tctttttgtc ccagatgata ttgacaccga cacagaagat gagggcggag ccgataaagg
    61501 agagattggc aagggcctga tccgtcatca aaggttcaat caggcgggcc aaaagagtca
    61561 cagagccttg aaagagaaaa acgggtactg ctgaaaagcc tgctcccttg tccaaactcg
    61621 aggtcataat caaaatgata ataaagtcca gaatactctt aacggccaac aagcgataat
    61681 cacccgtcaa tccatcttgg atggctccta gtatggccat agcaccgata cagacggtca
    61741 aagaagctgt caggaagcat ctaagaattg accatcttgg ctgtttcctg acctttctcg
    61801 gagccagata cccaagcgct caaaccaacc ttcgatatgc agtacttccc caatcaaggt
    61861 ccccagggct agactaagaa ccaaaagcat gctattttga cttttgagtt gaccatcaac
    61921 gactgtcagc agtccttcta aactacctga aattcctaga aataaaacag ctagcccgct
    61981 cgccaccgtt agtcctgact gatgccgttc tttcagtaaa tggccaaacc aagatcctag
    62041 caaacctgct agggcaatgg ccaccgtatt gataatggtt cctaatgcga acatgatcct
    62101 cctttatgat tcactatcat ctggcaatag ataataaact tttccatctt caaccaacaa
    62161 tttcacattc tccccataat tgttaacaga caatcgctta ctagattgat aggaaacagt
    62221 ttcttgttct gaaattagct taactgtccg ttttcgaaca tcaatgtaac gaatggcctt
    62281 atttagctct ccagccgctt taccaattcc aaaacctgct gctgcaccaa caaggcctcc
    62341 aagaaagcct actccagcag caacatgggc attcttatcc aactcttttg caatatctgc
    62401 aactactttc ataggaccct cttgggtacg tctattgcgc ttagtgtcaa tttggaaaaa
    62461 ttttatctta tctttgtgcc aattggctat cttttcacct agactattac aattgtttag
    62521 atagtactca tacacatagt aactactatc ctctgacgcc actccttgcg agaagactag
    62581 atctagctta ctaagttccg ctaataatct caacaaggtt tgttgataga ataaattagt
    62641 acttaactta tcaattaact gttcatactt ttcaaaagat aatccttttc ccttagtgat
    62701 actctcttca atagctagat tgacctgttc tagtaacttg gttacttcag cacggaagtt
    62761 agcacactcc atcaacttta gtcttgcaag ttcagcattt tcgacatagt ctgctgaaaa
    62821 ttttgatagt tgagaaatat taattatcaa agcgcgaaca gtccctttaa attcccttac
    62881 ttgaaaatcc ttaacatcgt tcaacgtttc tttcattgaa tcaagctgac cattgatttc
    62941 agccatataa tactgaccaa ctaccatagc agcgacctgc atagctcctg aaacagcagc
    63001 tgcagctttt gctccttttg caatttcaac ttgagcagga gttagtattg catgagcctt
    63061 aaattttcct tccgcacctt taacaaaagc acgatactta tccgtttccc ctttgatttt
    63121 gtgcaaatcg cctatcggca aatccacttt aaacaatccc ttaggaatat taggctgtcc
    63181 ggctttctta agatgatgtt taagtgctgg ttgaactatc ttttcaacat gagccaaaat
    63241 attcggattc gtaatttcat atctctgata tgctgatgaa tccaactcac tcattttctg
    63301 aatgagagca aagcaatcat ccgaaacaat caattctcgg tgttcctttt gttctaattc
    63361 actcatctcc cgattctcct tacctcaaca attccgtcgc gtaactaata gcagaaaggg
    63421 cgtagagggg agccgtttct gctcgcaaga tacgaggacc aagacctgtc ttgaccgctc
    63481 ctgctccttc aaatgcggct acttcctcag gcgacacgcc accttctgga ccgacgatga
    63541 caaggactga ttggccgatt tctaagccag acaagatttt caccaggttg gcttgttcgc
    63601 cctcttttgc ggcctcttcg taggctaaga taatctggtc aaagcctgct agttgagcta
    63661 ggaaatccac ttttttctca aataaacgaa cagctggaat gcgattgcgt ttgctctgct
    63721 cggctgcgcc ttgggcaatt ttttctaact tttctgcttt tttgcctaat tttttaccgt
    63781 cccatttgac cacggaccag tccgctggaa aggcccagag ggctgccatg cctaactccg
    63841 ttgccttttg ggcgacaaat tcgagcttat ctcccttggg aaagcccatg gctatagtga
    63901 cggaaacagg taattcgaca ttgtctgaca gttcttcgat gatttcaaca gattggttct
    63961 ggctatctac cacgcgcgcc aagcgtttaa tcccgtcgtc aaagactagg acaatctgtt
    64021 catctgcctg caagcgcatg acagaaaaca tatgcttggc ggtgtcctta tcgcttatct
    64081 ggaacacgcc ctgcggtgct ctgccattga taaaatactg ctgcatccta gcctccaatc
    64141 acacctgagc ggtcagccgt cttcttaaag acacagcaat tccactcgcc ctgaatcata
    64201 tgggtctcaa ggaagaaacc agccgcctcc gcagatgctc gcaccatgtc ccacttatcc
    64261 gcgatgatgc cgctcataat caggtagcct tcgtccttga ccagacggta ggcatcctcg
    64321 gtcagatgga tgagaatgtc cgccaagatg ttggcaacga taacttctgc tttaatatct
    64381 acgccacgaa gcaaatcgcc cgctgcaaca tgaatattgc tagtattagc gttgaggtca
    64441 atattttcct gagccacacg caccgcaact tcatccaagt cataggcata aatctccttg
    64501 gcacctagga gggagctagc aatagaaagg acacctgaac ctgtgccgac atcaatgact
    64561 gtctcgccac cacgcaagac ctgagaaaga gcaaagaggc tcatcttggt cgtcgggtgg
    64621 gttcctgtac cgaaagccat gcctggatcc aagcggataa tcttctcccc agctgtcgcc
    64681 tcatagtcgg tccaagacgg tacaatggtc aaatcgtggc taatgcgggc tggctcatag
    64741 tatttcttcc agttgtccgc ccagtcttcc tctgccaatt cctgactttc taggctgacc
    64801 tgacctgttt ccaagccaaa gcctgtcaaa tccgccaagc gagtcgccaa gtctgcctta
    64861 acttcctcaa tatctacagt atcaggataa taggccgtaa tcacaatcat atccgactgt
    64921 tccacctcgg gatagagttc accaaaacgg tcctcctgcc ccacatagtc agccgagtcg
    64981 ctaatagcca ccccctgact gcccgtttca atcatgagat ttgaaactgc ttcttctgca
    65041 tcacgatgca cgtgaatcgt taattcttgc catgagttca tgtttgttgt tccttttctt
    65101 ttctatgcgt caatcactgt attattgtac cgatttctag cctattttac aagttaatgt
    65161 tacgcgatgg agccctaata atactttttc gaaaattttc taatcgcact gcgaataaac
    65221 atgtgtagga aagtttgaga cggtggttcc aagtttctga acggaggtga tgcccatgaa
    65281 agtggacagc aaatctgcta gaaaggctag ctcatgccag atggtaagta aacttactca
    65341 tctggcaacg acaaatccta taagatttgt ctagggcacg cactagctcg agttttgaaa
    65401 aatatcgttt caaaactctc acgtcggaga actctggatt tatatttatc aagtctactt
    65461 gttgaaatgc tgagcttgat aatcgtcatc attttagcta ttcgagataa aaaataaccg
    65521 tcagcaaact ttgacacagt tctgacggtt atttaatcta acgaactgaa ccatcgttat
    65581 ggtcctgtca gggcgttgag ctggaactca acgccttttc tatttttata atagcaaaag
    65641 tttcgcctgc agtcaagcaa gaaagaaaac ggaatgtcaa atagaatcta ttaccctcaa
    65701 ttctatacca actgcaccgt actgttctaa ttgttctcgt gaatagtaat cttccatatc
    65761 ttcgggtttc gcgcctctga tactgctttg cgtataccca caagacaata aatccagttc
    65821 tttgtataac tgagcaaaat tatcaaatat gtgaagatct aatacttgca cctcgatcgt
    65881 ttgtgtctga ttttctgtac aataaaaacg gatgcgatct ccaatttgta tatgcttgcg
    65941 cttctcgtca taaagtcgta gctcaatggt cttctgacca gacttcatca tttcaaaagg
    66001 ttttggagcc agtaacattt catgaaccaa ctcaatacct cggatatggg taaaattctc
    66061 cctccaacaa gtctgctctg cctttttcaa aagcctctcc gtcccaagaa atcgcaaaat
    66121 cttgggcttc tgggtccacc aaaaaatcca tgaggtcatc taaaccttgg tcggcggtat
    66181 ctttgagaaa gcctgcgaag taggtcagaa actccgctga aaagcccttc ttggcgtcaa
    66241 atggcagagc gaccaggtag tcttcctcgt caaagcgaga cttgtcctgg ttgtagaaaa
    66301 ggacatactc ctctaggaaa atatcctcag aagacgcatt accttcgtcg tccaccgttt
    66361 caatcccagc cacattttgc gcttccaaga taaaagccaa ctcaactgcg tggtttttct
    66421 tgtcccagtt gagctcgtaa tcgtaggtaa aatgtttgtc cagtgccgct tccagcacat
    66481 ctaaaaatcc atgttttgcc atgtcagtcc tctttcgatt ttgtgttata ataagtgtac
    66541 cataatcagc taacaaaaga aacgaaagga agcctatgcc agccaatctc gcccttcgta
    66601 tgcggcccaa atccattgat gaagtcatcg gtcaggaaca cctggtcggt cctggaaaga
    66661 ttatccgtcg catgattgat gccaatatgc tgtcgtccat gattctctac ggtccgccag
    66721 ggattggtaa gacctcgatt gcgtccgcca ttgctggtac gaccaagtat gcctttcgaa
    66781 cctttaatgc cacgacagac aaccaaaaac gcctgcagga aatcgctgaa gaagctaaat
    66841 tttctggtgg cctggtcctc atgctcgatg aaatccaccg cctcaacaag actaaacagg
    66901 acttcctgct tcctcttttg gaaaacggca atatcatcat gattggagca acgacggaaa
    66961 atcccttctt ctcaattttg cctgccattc gcagtcgggt gcagattttt gaattacaac
    67021 ctttgaaaac cagtcacatc cgacaggcct tggaactggc tctgacagac agcgaacgtg
    67081 gttttgattt tcccgtcacc attgaacctg aggccctgga ttttctggca aatgcgacaa
    67141 acggtgacct tcgtgccgct tacaattctc tagaactggc tgtgctttcg accaaggaaa
    67201 gtgacgacgg tagccgccac attgatctgg acgccgtgga aaatagcctg caaaagtcct
    67261 acatcagcat ggacaagaac ggcgatgccc actacgatat cctctccgcc ctacaaaaat
    67321 ccattcgagg tagcgatgtc aatgccagcc tccactacgc cgctcgtttg attgaggccg
    67381 aagatctgcc tagtctggcc cgtcgcttga cggtcattgc ctacgaagac atcggcttgg
    67441 ccaatccaga ggctcagatt catacggtga cggcccttga agccgcccag aaaattggct
    67501 ttccagaggc acgcattttg attgccaatg tggtgattga tttggctctt tctcccaagt
    67561 ccaattctgc ctatctggct atggatgcag ctctggctga tttgcggaaa aacggccatc
    67621 tgcccattcc aaatcatctt cgggatggcc actatgctgg cagcaaggag ctgggaaatg
    67681 ctattggcta ccagtatccg catgcctatc ctgaaaaatg ggtggaccag caatacctgc
    67741 ccgataagtt actagctgcg gactacttca ccgccaacga cactggaaaa tacgagcgtg
    67801 ccttgggcat gacacaagaa aagataaaaa atttgaaaaa aaatagacgc caaaatcctt
    67861 gatagaactg gtttttgggt acacacgctt tgacaccgct tacatttttg ccatttttct
    67921 cttgattttt caaaaaaaag tggtattatt aaatcataaa taagaactgc tgtggtatac
    67981 gaattcatac ctatgtgttg accgactatt tttgtattac tagggagaca aagatcctct
    68041 aacagtatgc aagctggcac acccagaagc aactaagtta gaaacgcgtc acccacctgc
    68101 ttttagcgtg cgggatcaat acaatccatg gataaccggt accaatacag catttttatt
    68161 ttttaccttc cttagctcag ctggcagagc agcggactct taatccgtgg gtcgtaggtt
    68221 cgatccctac agggaggatt ggttgatatg aggaaagtct tgatttatca aggctttttc
    68281 tcgttttttg agtgaatttg gtcaaaattt ggtcaaaaag aaccctttca cattgtgaag
    68341 ggctattttt ttaacttatc ccacaggaca acacgttttt cctgtaatga tttctgtaca
    68401 tcgatgatgc gttggttgga agagcctcga aattgcagca taagattgcg cttggtacgg
    68461 tcataacggc cgtcaactag aatatctaaa taggatagta actctaactt atccggagtt
    68521 tctaacatca tttcttccca tgtgtagcct gtccaagacc aaatgtcctt ttctggcagt
    68581 tctttccgaa ttcgctttac gagtgggagg accgtgcctg tgttcagaaa aggctcgcca
    68641 cccaagagag ttaagccctg cacatagggc tggcccaagt ctgctaaaat gcggtcttca
    68701 agctccttgg tatagggaat ccctgcttta aaagaccagg ttgcaacatt gtagcagccc
    68761 tcacagtgaa acagacagcc actaacatat agagagcaac ggactccttc tccatccaca
    68821 aagttaaagg ctttgtagtc cattattcgc ccttggctaa gttcctcgct cttccattct
    68881 tgtggtttag gattattcat ttcaaatacc tgagcataag caaatgaaat ctgatcgttt
    68941 ggatgaattc cgttttacca gtacacagac tgtttttatc cataaatggt acttccaatg
    69001 ctttagctcg gattgatccc agcaagctta gtgcggaacc aatctcaatt cctttcctgt
    69061 tttgattcag cagtttgtca ctcccattaa tcctcaatcc agtatctttc tactccgtct
    69121 cgaacatctt ccaaaacgcc accacaagcc acaatcgtgc gacggctggc ttgattgtct
    69181 gtcgagcaag tcacaaggac cttggaaata tttttggttg cggcttcctg taaaccaagt
    69241 cgcagctggt ccttggcata gccctttcct cgctcggtcg gacgaatgga atatccgata
    69301 tgaccgcctt tattgagcag gaaatcattg agccgtaggc gtagactgag aaaaccaagg
    69361 gctcgaccag tctggtcaaa tgacacatat tgaatggaag gaacaaaacc gtctggcaga
    69421 ttgagacctg cctcatagtc tttgtttcta aaaatccatt cttcaaagtc cacatctttg
    69481 gagataaagc ctccgtccat agctgagcct gccgcttcaa actctgccag catttccaaa
    69541 atcgtttcct tatccgctat agtcggtctt cttagttcca ttctagcctc catattgtct
    69601 cttgaaaaat tcactgatga tgtctatatc cagtcccaag tcctgcacaa tttctgcctt
    69661 gtggaaatca tcatagttgt ccttaccgct ccaaacaaaa gaattggtca caatctgata
    69721 gtccttatct gactcgactg ctctgccatt gaccaaaaga tcctcaccgc tttggacaat
    69781 gccccacatc tgagggtgga ggcctgtctg gagactggag agtaagtctc tgcccttgat
    69841 acgaactaag agtactcgct tggaaaaggg caagaccttg accaagtccg aataaaagat
    69901 tggtcccgcc gacaaggaag ctcgaatacc gaagccgttg atgaccgctc catccgcctg
    69961 caaacctagt tgactcgcct gttcaaataa agcctgacaa ataacagggg ccagagttga
    70021 taacccttga cgaatgctgt ctcgctctcc gtctagtgcc tgaggtaaaa tcgctatcgg
    70081 tttggaaaaa gctgcgtcac gttccgattt catctggtcg ataatagcct tgaccgccct
    70141 gtcttcttgg gtcagtttct ccacctcaat caagtcataa gcctgaacct cgtgccgtcc
    70201 atctgcaaaa caccgcaggg atagatgacc gacaaagcga ccatactgac cagcttggca
    70261 gatactaact ccgccaatct gactaggttg ttttagaatg gtatgggtat gtccgcccaa
    70321 aatcacgtta agctcaggga aatccttggc caaatgcaca tcctcatcgt atcccagatg
    70381 actgagcaag accagatgag ccgatggatt ctgactcaca atctgatcca ccaatcgccg
    70441 cagagcttgt ttatggtctt caaaaatcac attctccgac ggtgacgcaa cctcctgggt
    70501 ttccaaggta gtcaaaccga ggacatagag gtttttccca ttcatatcaa attcaaggac
    70561 atcctcaagc ggcaccaact catctgccac ctccacatcc tgatagcgga gattggagga
    70621 cacaattgga aactgggcat agtcatccaa gcgactaatc aactcatctc cgtggtcaaa
    70681 ctcatgattg cccaaggtca tagcctgaca accaatttga ttcattagct cgatttcctt
    70741 gactcctcgg tacatattga aaaagatatt acccgaaaag agatcgccgc tgtcaaagac
    70801 aaaggtcgga actccctctt tttcattcct agcccgaatg ttcgcaatca gttgcgcctt
    70861 ctttggaaaa ttctccatgt aggaatgcat atcattggta tggagaatgc taatatctgt
    70921 atagtccata ctattttttc ttattttttg cttgtttgag caattcctgc acacgcgctt
    70981 ttttatcctg cttcacatta gaattcttct cgtgatagcg tgtcaccagg gccttcccct
    71041 tatcgtctaa ttgatatttt cccattgttt tctcctcttt ttttatctct tatttgttta
    71101 taataaactt ccacagctaa ctggaactgt ggaagctcta catagtctgt ttattactta
    71161 taagctcgac ccattcatgt gtttcacacg cgccgaaatt tccttgtgac ggccttttac
    71221 catcggacgg gcctgtggat tgcctagata accacaagtt cgtttaacaa catccaccga
    71281 cttaggatcg ctattcccgc aatttggaca cttgaagcca cgctccgtcg gctcaaaatc
    71341 accttcaaaa tcgcaagcgt aacaatggtc aattggagta ttggttccca agtaaccaac
    71401 acggtcataa gcatagtccc aaacagcttc caaggccttt ggattttgtt gcagcacagg
    71461 gtattcacag taatggataa atccaccact ggcacctaca gcaggataaa ttttctcaaa
    71521 gtccagtttt tcaaacggtg ttggattttt gcgtacatca taatggaaac tgttagtgta
    71581 atattcttta tccgtaatgt ccttaacgat gccaaatttc tctgtatcca tacggcagaa
    71641 gcggtctgtc aggctttcag atggtgtcga ataaactgag aaatgataac catattcatc
    71701 cgaccaatct tcaaccaagg ctttcatttt cttgataata tccaccgtga aatcttttgc
    71761 ctctgggttg gtctcccatt cgccaccgta aaatactgtt gcgacttcat acaaaccaat
    71821 gtaccccaaa gatacggttg ctcgacgatt tttaaagact tcatctactg cgtctgtctt
    71881 agccaagcgt ttgccgaatg ccccatactg atacaaaatt ggtgcattgg caggtgtcgc
    71941 ctccttgacc cgctccacgc ggtaaaccaa ggcatcctta gcaatggcca tacgttctac
    72001 aaagatttcc cagaacttat ccttatctcc agcagactcc atggcaatcc gtggcaaatt
    72061 gacggttacg acacccaagt tcatccgacc agatgtcaca tcctgcccat tttcatcctt
    72121 ccaaccttga aggaaagaac ggcagcccat tggcaccttg aaagaacctg tcaactcaac
    72181 aatcttgtca taagaaagca tatctggata catacgcttg gtcgcacatt caatcgccaa
    72241 ttgcttgatg tcatagtttg gagaatctgg ctccaagttc aatccacgct taacggtgaa
    72301 aatcaactta ggaaagatag ctgtccgacc ttctcgccct agacccttga tacgaatgtt
    72361 caaaatcgct ttttgaattt ctcgctcaaa ccatgatgtc cctaacccaa aaccgagtga
    72421 ggtaaagggt gtttgtccat tggaagtaaa gagggtgttg atttcatact ccaaagactg
    72481 catagcatca tagatgtctt tttgcgtttt tgcacgcgca tattcttctt gcttgtctgg
    72541 caatacccaa tccttggcgt ctttcaaatg cttttggtaa ttgagttccg cataaggcgc
    72601 caaaacttca tcgatacggt cagctgagca accaccatat tgactagaag ccacattggc
    72661 aatgatttgt gaaatctgag ccgtagctgt ttggatagac ttcggacttt ctacatcggc
    72721 atttcctatc ttgaagcctt gtgccaacat tcctttgaaa tcaatcaaac aacagttggt
    72781 cattggtgta taagggctgt aatccaaatc atgataatgg atgtcaccct tttgatgagc
    72841 attggctaca tgagctggta ataatttaag accgattgac ttgccgacaa tccccgctgt
    72901 caggtcccgt tgagtattga acacatccgc gtccttattg gcattttcat tgaccacaga
    72961 ctggtccttg ctcaacagct tatgaatctc aaaattaata tcggtagcct gatgacggcg
    73021 aaaatcacgg cgtgttcggt attgaatata aagctccgcc aaatcatata aatgagcctt
    73081 taacaattcc tgttcaacga ccgtttgaat ttcgtaaatt tgaacatctt tttcaaatcg
    73141 gcgaccaatt tcagccaaaa cagcttctaa tacaattttt aaacctgcct ctgaaacagg
    73201 atgttctaat tcttggtttg cccgccgaag agcagaaaaa atcttatcct ggtcaaagga
    73261 tactgtccgt ccatcacgtt ttaaaactaa taattctata ctcggtaaga cattttcttg
    73321 cacattcatc tgacaaacct ccaaaattct tcacttctat tagtgtagca cttcacaaaa
    73381 caaaaatcaa tatcttgtgt aaaaaaatat taaacaacct atccatgcac tatatatagg
    73441 tcttgaaaat tctatcaatt ggaaaacaac tgtgccaaat ttcaccactc gacaaggatg
    73501 aattactaga ttgaaatatg acaacagatt gggcaaaggt ctaacttctc tatcagaaaa
    73561 cctaaaaaac agccctctaa cagggctggc tctatgtatt gaaaataggc tagggcgggt
    73621 caccacatgc ctatttaaaa ttgaggattt gaataagagg cgaaaaattc tctagtccgt
    73681 tcttctttag gatgatggaa aagctcctca ggtgtcccct gctccaggat acggccgttt
    73741 tctagaaata ataccttatc cgccacttgg taaatgaaat tcatgtcatg actaactaaa
    73801 atcatcgttt gtcctgtctt agccgcatcg gcaatggaac gttctacttc tccaaccaac
    73861 tctggatcaa gcgccgaagt cggctcatct agtagcaaaa tgtctggttg catggcaaga
    73921 gcgcgtgcaa gagccacacg ttgcttttga ccacctgaca gatgtcttgg ataatggttc
    73981 tcacgttctg acagaccgac ctttctcaat tcttctttgg caagggcggt tgcagatgcg
    74041 tcatccatcc gcttgacaat tttaagccct tccttgacgt tatcaagagc tgtctttcgt
    74101 tcaaacagat taaactgctg aaagaccata gccagtttgc ggcgtaaggt caatatctcc
    74161 tcttttgaaa tattggtaaa atcaactgta aaaccatcaa tttcgatact ccccgcatct
    74221 gcctgctcca gatagttcag actacgcaaa atggttgatt ttcctgctcc agatggtcca
    74281 acaagggcaa taacctgccc tttttcaatg tccatatcga ttccatctag tactttttgt
    74341 cctgaaaatt ccttgactaa attgcgaatt ttaatcatta gcgatcacct ccaactccag
    74401 aaatatctac tgattcaggg gcctgaatag ccattttatt ttcaaggaaa cggccgaaag
    74461 actcaatcag aacagagagc gcccagtaga ccaaagagac agaaatgtac cgctcaaagt
    74521 agcgatagtc tgccccgcct tgaatctgtg cctgtgcata gacctcaacg atacccgcac
    74581 tgaaggctag ggaagttcct ttagtcagac caatcaaggt gttaatcaac gacggagttg
    74641 caatcacagc cgcattagga atgatgatcc gacgataaac ctggccagaa gtcataccta
    74701 aggaacgagc cgcctcaatc tcccctggac taacagcctg aatcgcagca cgaatagact
    74761 cactcatata ggctgcctca ttaaatgcaa aggctgtaat cgcaaagaca gctgcaggaa
    74821 tagcattgat attccaatca agaccatatt cctgattgag ccctctaaga accaatggaa
    74881 ttccgtagta ggtcaacatc agctgcacca agataggagt gcctcgcaaa aaactaacaa
    74941 aaactgcctg aacgggatat aagaatcgca cacgattaat ctttacaatc gcaaaaacca
    75001 agcccaatat caaaccaaaa aaagcacctg cgagcgtcaa gccaatcgta atcggtaaac
    75061 gttcaataat ggagggtagg gcatcaaaaa cagccctaat actaaatagc ttaccatccg
    75121 gtaaccactc taaaaaacct tgataccatg ccggtcctgt tattcctaat acaccaatca
    75181 tactcttctc caaacattta aaaatgacag attttctgcc gtatactcta ttttatcatg
    75241 taaccaccca catcaacctt atatatattt tatggctgtg ataagtaatc ttaatgatta
    75301 taaaaagaca atgaaaaaga aacagcccac ttggactgct ccctcctgtc acaatactat
    75361 cgactcctat tcattaggaa cagctagttc ctccgccgtt ggagcatagt cttttccgaa
    75421 gaattgctct gtcaatttcg caatggtccc atcttcgttc aaagccttga tacggtcgtt
    75481 gacaaagctt tgtagagccg tattgtccga agaaaataca tagtaaataa acggtttttc
    75541 tgctgcttca agctcgattg ttttaagatt tgtcaaattt tgatctgcaa taatagcatt
    75601 aaccgtaggg gcatcaaaaa ttttaaaatc atacttgcca tcatttatat tggtcaaaat
    75661 ctgtgtgata ttttcaccag tatagttgat gtcgactggt ttatccgtat gctgagtatt
    75721 gtattcctct agctgagcag ctgttgttgt ccctgtaact acctgtgtag aatgaccacc
    75781 aatatcatca taagactgaa tatccgtatc ttttggcaca gctaaaacgg atggtgtaga
    75841 ggccgttgga taagaataga ggtatttttc accacgctct tttgtatagg aagtattatt
    75901 ccctaacaaa tcaaagcgtc ctgaatccaa gccagggaat tgaccgctac catctgttgt
    75961 ttcaaaggtc aattcatact ggtcagaacc atcaaagata gctcgagcca attcaatatc
    76021 aaaccctgtc aattctccat tctcatctcg ataagaaaaa ggcttggtag tcccaacagt
    76081 tcccattacg atggtttctt ttttatccgc ttctgaagaa ctagtgtttg atgagtttga
    76141 ggtagacgag caagctgcca aagcaactgt cgcaagagct gtaataccag ccagtgatag
    76201 aatagttctt aatttcatag taattctcct tttgtataag caagtcagac ctacaaggtc
    76261 tgcacaaaat aatcaaatag cttctgattg ccgccacttt gatcaatcag aagttccgga
    76321 tgccactgga cacccagaaa acgtgtctca tccattgatc ggacagcctc aataatacca
    76381 tcatccacag aatgcacaat cgcttctagc ccctgaccca gattctctat cgcctggctg
    76441 tgaatagaat tgacagctac tctctgacca aaaatactag ccagaggcgt tgcctcttta
    76501 cattctacct ggtgaatcag atcaaaaggc aaggaccgat gcccctctac cgcttgtttg
    76561 agagttcctc cacgtgcaac attgaaaagc tgcatccccc tgcaaatccc caaaatcggt
    76621 ttttcctgtc tcaaggcttc gtggaccaat ttcagttcaa attcgtcacg ttccttgaaa
    76681 tagtcatcgc tttcgatgag tttttcttga ccgtaaaact gaggtaatac atgttgacca
    76741 cccgtcagta ataacttatc aatcgccgac acatagtcga caacatccgt ttcaccagac
    76801 aaggggatat aaaaaggaag gccccccgct cgcttgattc catctgcaaa gttacgagac
    76861 aacgataaat tcataatcgg aatcccctct gcgacaggct tttcattact ggtgatgcca
    76921 ataatcggtt tccccatcct gccctccttt cgtaatgtat agacactatc ttatcatgct
    76981 ctctcttttt cgtatatttt aaattattta tagccagcca taaaaaatat ttatggatca
    77041 aaaaaagagc ctattctgac tcttgttttc tagtccataa aaagtacagg ttaatagcac
    77101 aggccactaa aatcacagca ggccaaagca agttgatgac tggaattctg ccaacattta
    77161 ccgcaagaaa gaggatattc cacaccactc ccaagctata gacaatcgtc aaaataattt
    77221 tcataaaaca ctccaacaaa atgatagtgc tattatacta caaacgaccg ttaatgtcct
    77281 tgatttgccg ctccattttc cgatggttta ggaaaaatag caaagcgtat accaaggtaa
    77341 agataaacca gaagaggtag ataagttcct tgtccgacaa tagaagaggt tatgagagaa
    77401 ataatggttc cgagaataac acctaaactg actaaacggc aaaaaagcca gaacaattgt
    77461 cctgactttc attgtcttat atttttttct tcaactccgc cactgccatc atgacttcca
    77521 taggggtcat attgtagata tccagttttg ccagttcatc gaggactggg tgagaaggcg
    77581 tgtctgcgaa gaggtcgatt tgaccagacg gctcttccac gactatcgga gctggggtag
    77641 ttggtgcagt aggagcttgg ttttcaagcg tctgcaaaat cctgtccgcc cgctgcaata
    77701 gttcctctgg cattcccgca atcttagcca catgaatccc gtaggacttg tcagctggac
    77761 cttgggcaat cttgtggagg aaggtgactt gaccgtcctt ctccaaggtc gatacatgga
    77821 cattctctag gtgttccaag gtctggctaa ggtctgtcaa ctcatggtag tgggtagcaa
    77881 agagagtctt ggctccaatc ttgtcgtgga tgtactcgat gatagactgg gccagggcca
    77941 taccgtcata ggtggccgtt ccccgcccca actcatcaaa gaggatgagt gaccgatctg
    78001 tcgccagacg gactgcctta ttggcctcca tcatttcgac catgaaggtc gattgaccac
    78061 taaccaagtc gtctgccgcc ccaatacgag tgaaaatagc atcgaaaatc ggtaattcag
    78121 cctgatccgc aggcacatag gaacccatct gcgccatgat gacgataacc gccaactgcc
    78181 gcatataggt agacttacca ctcatgtttg gaccagtaat cagttgcata tgcgtatcgg
    78241 tattcaagtg aatagagttg ggaatgtagg tctgcttgcc catgaccttc tccaccaccg
    78301 catgacgacc acggtcaatg gtcagttctc tttgatctgt aaagcgtgga caaaccaaat
    78361 gttgctgctc cgccacaact gcaaaggcct gcaaaacatc gatggttgca atggtccgag
    78421 ccagcttctg caagcgacct atatacttct caacctcttg gcgaatccgc atgaaaatct
    78481 cgtactccaa attagcagac ttatcacgcg cctccaacat ctgcccttca atcttagcca
    78541 actcttccgt tccatagcgc tccgagtttt tcaaggtcgc cttacggaaa aaatggtccg
    78601 gcacattgcc cagattggaa ttggtaacat ggaaatagta accgtctttt ttattgtaat
    78661 caatcttaag attattgata ccagacgctt cacgctcctt agcctcaatc tccgcaatcc
    78721 aacccgctcc ctcacgaatg accagacgat actgatccag cgtttcatca aagcccgtac
    78781 ggatgatgtt tccatctgtg atggtccctt gggcttctgg gtcaatagca gagctgatga
    78841 gagcgtgcag ttctgggatt gggtccaatc cagccaccaa attgcctaga gcaggctcat
    78901 tgatttgttg caagatattc ttgattgctg ggatatttcc cagagtctgg gagagctgca
    78961 agagatcctt tggcatggtt tttccaaaag atacccggct agccaagcgt tcgatgtcat
    79021 agacaccttt taaagcttca accaagtcac tccgttcaaa gaagtaatcc agaaaaacct
    79081 gaacgatagc ctgacgattc tcaatccgct taaggtcaat caagggacga tcaatccatg
    79141 tccgcaatag tcgcataccc atggctgtct tagtttcgtc taacaaccag tacaaactac
    79201 catgcttctt gcctgtccga ccgttttcaa gcaggtccag actggacttg gttgcatagt
    79261 ccatttgcag atagtccttg atttcatagt ggaccacctt ttgcaagtgg ctcagatctc
    79321 gcatctgagt ccgatgtagg tagctgagga gtttgcccgc cgcagccttt tccaaatctg
    79381 tcaaggagtt atcaatcaat tggacatcct ctgtcacctc atcttcaaaa gagagtagga
    79441 ggttcatctg gtttgaaaag acctgctctt cttcctcaga taaagcatag ccgatgacca
    79501 attcacgtgc ccgtaaattg cggatttcac cgcaaagact agtaaaatca tccaaactag
    79561 tcacaaaaaa ctgacctgtt gacacatcca tataagaaag tgcgaactgc actccctgac
    79621 ggtcaagcgc aaccaaatag ttactgtctg ctcccatttt ggacgagtct gttaccgtac
    79681 caggagtaat gacctgcact acttcccgct tgaccacgcc gactgcctgc ttaggatctt
    79741 ccatctgctc ggcaattgcg accttgtgcc ccaattcaac aagtgtgtcg atatactgtt
    79801 gtgccgcatg atacggtacc cccgccatag gaatcggatt ctccgcattc ttattccgac
    79861 tggtcaagga caattccaaa atctgcgccg cttctaccgc atcttcataa aataattcat
    79921 aaaagtctcc catacggaaa agcaaaaaag catctggata ctgtgctttt atatccagat
    79981 actgctgcat tcccggagaa attttttctg ttgccatcat ctctccctgt tctaattcaa
    80041 atagtagtta atctccgctt caacttgctg agccgctaca tcatctcgac aaataactaa
    80101 aagtgtatta gctcccgcca gcgttcccaa aatagtctct ggcttttctg catcaatgat
    80161 attggccatc aaggccgctt ctcccagttc tgaatgaagg accagaataa aactagccct
    80221 atctacttta tacgcatatt gtgccaataa ttggataaaa ttcacgcgct cttcttcgat
    80281 agctaaggaa taaaatgaac gcccattttc atgaattttt atcactccca actcacgtaa
    80341 atcacgagac aaggttgtct gggttaccac tacccctcga gcctctaaac cttgctgaat
    80401 atccgtttgt cgccctattt tttcttgacg aatcatggcc tttattaaat catgtcgtcc
    80461 tgctttattc atcattttac ctcaaaatgt atatttatag aaatattata acacttttcc
    80521 ccgctattag cctagaaatg tgttaaaata gaatcaataa ctttggagga aatcctatat
    80581 gaatcaaaaa caagtgattg cagaaagatt ggctgccatc cttccgagtt tggaagtgga
    80641 agctatctac aatctgctag aaaaaccaaa atcatcagaa atgggcgata ttgccttccc
    80701 agccttctca cttgctaaag tggaacgcaa ggctccacag gctatcgcag cggacatcgt
    80761 tgaaaaactc gatacaactg gctttgagaa ggttgtagct accggtcctt acgtcaactt
    80821 cttcttggac aaggctgcga tttcccacca agtcttgaca gatgttatta ctgaaaaaga
    80881 ccaatacggt aaactcaaca ttggtcaagg acgcaatgta accatcgaca tgtctagccc
    80941 aaacattgct aaaccattct cagttggaca tttgcgctca accgttatcg gcgatgccct
    81001 tgccaacatc cacgaaaagc ttggctacaa gccaatccgc atcaaccact tgggtgactg
    81061 gggtaaacaa tttggtatgt tgattgttgc ctacaaactt tggggtgaca aggctgcggt
    81121 cgaagcagac ccaatttcag aactcctcaa actctatgtg cgtatcaacg ctgaagcgga
    81181 agaaaagcct gagttggacg acgaagcacg tcaatggttc aaaaaattgg aagatggcga
    81241 ttcagaagca cacgagctat ggcaatggtt ccgtgatgag agcttggtcg aattcaaccg
    81301 catttacgat aaactcgatg taactttcga cagctataat ggcgaagctt tctacaacga
    81361 taagatggac gaaggcatcc aaatcttaga agaaaaagga cttcttcaag aatctaaagg
    81421 tgcgagaatc gttgaccttg aaagctacaa tctcccacca gccctcatca tgaaaacaga
    81481 cggtgcaaca ctttatatca cacgtgacat ggcaacagct atgtaccgca aacgcactta
    81541 cgactttgtg aaaagtatct acatcgttgg tcaggagcaa atcaaccact tcaagcaact
    81601 caaggctgtc ttgaaagaaa tggatttcga ctggagcgac gatatgaccc atgtcacctt
    81661 cggtctggtt accaaggaca agaaaaagct atccactcgt aaaggaaata tcatcctact
    81721 cgagccaact ctagatgaag ctatttcacg cgcccttact caaatcgaag ctaaaaaccc
    81781 tgaccttgaa aacaaggaag aagtagcaca cgcagttggt gtaggcgctg ttaagttcta
    81841 cgacctcaaa accgaccgtg acaacggcta cgacttcgac ctagaagcta tggtttcctt
    81901 cgagggtgaa acaggtcctt atgtacaata cgcatacgcc cgcatccagt ctatcctacg
    81961 caaggcaaac tttgtaccaa gcgcagaaaa tgactacaaa ctggctgatg cagaaagctg
    82021 ggacatcatc aagcacatcc aaaacttctc aaacgttgta gaacgtgctg gtgacaaatt
    82081 tgacccatcc ctcattgcta aatatgctat caacctggcg caagccttca acaagtacta
    82141 cgcacacact cgtatcttgg atgaaagccc agagcgtgat agccgtctag cacttgccta
    82201 cgcaacagga cttgtcctca aagaagctct acgtcttctc ggcgtaaaag caccagaaaa
    82261 aatgtaatca aagccttcgg ccatcagccg aggctttttt tctaccacgt tgcctactct
    82321 cgtttccgat tccttatcac aacaactcct tatcccaaat gaaaatcaaa aagattcatt
    82381 tcaattacga atatatagat gggaggtaca cgtgatacct ctacatctac tacacaagga
    82441 gaaataatga aaacattctt gagaagaaaa ggaatcgcct gtcagcttct agccgccatt
    82501 tttattgtcg gtatcaattt acaatcccca ccttcagcct cagccaatgc acgaggagat
    82561 gactatccct acacagtagt tgatgcagtc gatccatgga gactctacac acgccagtgc
    82621 acctcctttg tagcctttcg acttagcaca gttaacggtt ttacattgcc acctgcctat
    82681 ggtgatgcca atgtttgggg ccatcgtgcc aaacaagaag gttatcaagt taatatgaca
    82741 cctgctgtcg gagccgttgc ctggtgggaa actcccatgc atgtcgcatg ggtttctgca
    82801 gtaaatggag ataccgtaga gattgaggaa tacaactacg gtgttcgcta tacctacgga
    82861 aggcgagtta tccccaaaaa tgccgttagc ggctacattc attttaaaaa cctcggagcg
    82921 ggaacaacca actctcaacc cgccgtagcc ccaagcggca cctatacttt taccagccaa
    82981 ctggccatca aatctcaagc tagtctatct agtcctaccc tcgattacta ttatgctggc
    83041 gaaagcgtcc gttacgacaa ggttctcgaa gccgacggac aacgctggat tagctatatc
    83101 agctattctg gagctcggcg ctatatcgcc atccagccta ccattactca atctacaaac
    83161 aacagcctac cagcatcagg tagctacacc atccagtcct actctagtgt ccgcaacaat
    83221 ccaagtctgt ccagtcctga aatcaccttc tacggagctg gctcagtcgt tcgctatgat
    83281 aaagttatta ctgccgaagg tcgcacatgg attagctata tcagttactc aggtgctcga
    83341 cgctacatcg ccatctccta atcacaaaaa agacaatatt taaaagcaca atctttttag
    83401 actgtgcttt ttcattaaat atctgtcgaa ttaatagctg tgcttggttt ttcctttggt
    83461 ttacgcagat ggaagaagac ggtcacacca aagtaaatgg caaataggac aactcgaata
    83521 atataattca tcattgcctg cgcctctact gactgacgca taactgaatc agtataaatt
    83581 tgactcaacc ctttctcacc aataaaagca taggtcgaaa ataggagagt tacaaaaaga
    83641 taacccaggt aggtataaga agcctgctca tttttcttga ggaaaagcag cacaatcgtc
    83701 gcaatcacag caagtaataa cacaactgca aatgccttat tgacaagatt cgtttggaaa
    83761 ttgacagact ctatcatata ggtaaaatat acctcatcaa gcccctctac attaaaattc
    83821 tgcaacaatt gacgcacagc ttcctcattg tacttcgcaa aaaatacatt aaagacacgc
    83881 aatacgctcg caatagatga cagcacaaca aagatataca aatgaattgg tcttttcata
    83941 atagcctcct tttttctata ttttatactc atatttttat attgtcaatc atatatttca
    84001 tttgactgca aatataattt tgttatacta agttcaaagg aggaaaactc atgaagaaat
    84061 tgcccaccta tatcggagtg attatttttg tagtcactct actgattaac tggttattgc
    84121 caagtcttgc taaagtgctg aatacatttt tcatttggac tatcgtagcc tgcctactct
    84181 acaccatcta ctactggatt cgctactggc tctatgtccg acaacagaga taacaaagac
    84241 tcctctaagt tatacttaga ggagttcatt ttattatgac ttttcatcaa ccaaatgcac
    84301 attaacccga cgataagttt ctgtaaggtc tagcaattcc gcttcaatct ctggtgtcaa
    84361 ttgatccgcc gtcattctcc aagtccagtt accaccaagc gtatttggca agttcatgcg
    84421 agctgaacca tccaaaccaa gtaggtcctg catagtagcc accgccataa aggcaggtga
    84481 accaaagagc aggcggaaaa gagcatgact gaccgtttcg ccctcacgac gattgctgta
    84541 cttatctaag aatgcacgcg attctttcgt tgtttcattt tcgtaccagc caagaatcgt
    84601 atcattgtca tgggttcccg tatatgccac cacattatta ggatggttat gtggcatttc
    84661 aatgctgtct ccttctggat caaaagcaaa ctgcaggact ttcataccag gaaaacctgt
    84721 cgtatcacgt aattggatga ccttgtctgt cacagagccc aaatcctcag ctatgatatt
    84781 taagtctccg agttcacgtt tgattgtatc aaataactca aaacctggtg cttcgacacg
    84841 atagccattt acagctgttt cttcacccgc tggaatttcc cagaaggagg caaagccgat
    84901 aaagtggtcg atacgtacca tatcgtatat cttaaaggat tcacgtaaac gagcaaccca
    84961 ccaagtaaat ccatcatgct tcatcgcttc ccaatcataa atcggattgc cccaaagctg
    85021 accaatttcc gaaaaggcat ctggtgggca tcctgcaacc acactcggct tgccttcttc
    85081 atctgtttta aagaaatgtg gattagccca catatcggca gaatcagccg cgatataaat
    85141 cggcatatcc ccaacaaact caacaccttt ggcattggca taagccttta aggccttcca
    85201 ttgtgaaaag aataaatatt gtgtaatgcg gtgataatca attcgatctg ccaacaagtg
    85261 acgataatat tccaaggtat cgtggtaacg taagcgcgcc ccttgatctt cccactctgt
    85321 ccaaggtttg aggtcaaaat gctcttttat agccatatac tccgcaaagg tatgaatcca
    85381 aaagtcattc tcccctaaga acatccagta atcttctggc aagccttgtg ccttcatgtt
    85441 gacaactacc ttctccagta atggacggcg agcataaaaa atggctgcgt aatctacctc
    85501 tgtaggattc tgcccaaagt caacaccgtg taaatcctct gctgctagcc agcctttatc
    85561 aatcaatata tcgaaatcaa taaaatgagt attccctgca aaagctgaaa atgactggta
    85621 aggagaatct ccataactgg ttgttcccaa aggtaagatt tgccaatagc gttgcctagt
    85681 tcttaccaaa aaatcaacaa attcataagc cgattgtccc atggaaccaa ttccatattt
    85741 cccaggtaat gatgaaatgt gcatcagcac accactctgt cttactttca taagttctcc
    85801 tatctaatcc gcttgcacaa cgcaaacgtt ttctcttttt tattatatac tgaaaacggt
    85861 aacaaatcaa ggaatttcca tttaattctg ttagttcaca tgccattcca tatttctttt
    85921 cattaaatat gtgcaaaaaa attcttatat ttttttaaac tcaaaaaaat ttttaaaaat
    85981 ttttataaaa acgcttgcaa acggattcaa tctagtgtat aatgaagtca acttaggaaa
    86041 acgtttgcgc aaagcaagcg tcacacaaaa ctatttatct tcggaggaaa gaaaatgaaa
    86101 cacaatctcc ttaagagcgt tgctcttctt gctgcatcaa ctgctgtttt agctgcatgc
    86161 tcaaactcag gttcatcaac agaggcatct aagtctgctg aaggtagcaa agaattgact
    86221 gtctacgttg accaaggtta tgaatcttac atcaacgatg tcaaagctgg cttcgaaaaa
    86281 gaaaatggtg tatctgttac cgttaaaact ggtgatgctt taactggttt ggataactta
    86341 tcattggata accaatcagg ttcagcccca gacgttatga tggcaccata cgaccgtgta
    86401 ggtagccttg gttctgaagg tcaactatct gaattgacac ttgcagatga tagcaaagca
    86461 gatgacacaa caactgctct tgtaacaaac ggtggtaaag tttacggttc acctgcggtt
    86521 atcgaaacac ttgttcttta ctacaacaaa gacttgttga ctgaagctcc taagacattt
    86581 gcagaacttg aaacacttgc taaagacagc aaatacgcat ttgctggtga agagggtaaa
    86641 acttcagcat tcctagctga ctggacgaat ttctactata catacggact tctttctggt
    86701 tacggcggct atgtattcgg tgaaaatggt acaaatccaa aagacatcgg acttgctaat
    86761 gaaggtgcca tcaaagcaat tgaatacgct aaaacttggt acgaaaaatg gcctcaaggt
    86821 cttcaagacg gtactgctgc aaacaacttg attaacactc aatttacaga cggtaaagca
    86881 gctgctatca tcgaaggtcc ttggaaagca gcttcatata aagaagctgg tgtaaactat
    86941 ggtgtagcaa caataccaac tcttgtaaac ggcaaaaact actcagcatt cggtggtggt
    87001 aaagcatggg ttgttccagc aggcgctaaa aaccaagaaa tggctcaaaa gtttgttgac
    87061 ttcttgactg ctacagacca acaaaaagca ctttacgacg ctacaaacga agttccagct
    87121 aacactgaag ctcgtgaata tgctgtaagc aaaaaagacg aattgacaac tgcagttatc
    87181 aaccagtttg cttcagctca accaatgcca aacatctcag aaatgggttc agtttggaca
    87241 ccagctggta acatgctctt cgaagctgca agtggttcaa aagacgctaa gactgctgca
    87301 acagatgctg tgaaagctat tgcagacgaa attgctcaaa aacacagcaa ctagtcaagt
    87361 ttaaagttgg actgggagtt ctcttcccag tctactttat tacttaacga taaactgaat
    87421 agatatagtc
//
LOCUS       DASFSW010000002        41039 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.002, whole genome shotgun sequence.
ACCESSION   DASFSW010000002 DASFSW010000000
VERSION     DASFSW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41039
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.002"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            13..1563
                     /gene="guaA"
                     /locus_tag="U1099_000080"
     CDS             13..1563
                     /gene="guaA"
                     /locus_tag="U1099_000080"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEM4866433.1"
                     /translation="MTKQDVQKIIVLDYGSQYNQLISRRIREFGVFSELKNHKITAEE
                     VRAINPIGIVLSGGPNSVYAENAFDIDPEIFELGIPILGICYGMQLITHKLGGKVVPA
                     GEAGNREYGQSNLQLKTESALFAGTPEEQLVLMSHGDAVTEIPADFHLVGLSADCPYA
                     AIENTERRIYGIQFHPEVRHSVYGNDILKNFAFGICGAKGDWTMENFIETEIEKIRQT
                     VGDKKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMEMLGGKFG
                     LNIIRVDAAKRFLDLLAGVSDPEKKRKIIGNEFVYVFDDEASKLTDVEFLAQGTLYTD
                     IIESGTDTAETIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTALGMPDEVVWRQ
                     PFPGPGLAIRVMGEITEEKLQTVRESDAILREEIAKAGLDRDVWQYFTVNTGVRSVGV
                     MGDGRTYDYTIAIRAITSVDGMTADFAKLPWDVLQKISVRIVNEVDHVNRIVYDITSK
                     PPATVEWE"
     gene            complement(1725..2774)
                     /gene="mcrC"
                     /locus_tag="U1099_000081"
     CDS             complement(1725..2774)
                     /gene="mcrC"
                     /locus_tag="U1099_000081"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HEM4866434.1"
                     /translation="MIPVQNIYYMLSYAFKILHKQEYKRIATEEFKNAADLLAEIMII
                     SLSIQVKRGLGREYRSQTESLSALKGKINISESLTPPNWRRKQLVCQYDDFSLDSRMN
                     RIIKASIEILLKADISRDRKKKLRKLLVFFGEVSKINLHSINWNLQYNRNNQSYQLLM
                     SICYLVVNGLIHTEREGNKKLMNFLDERRESLLYEKFILGYYKKHYPQIQVTASQIPW
                     ALDDGFGEMLPIMQSDIYLKYKDTILIIDAKYYSSNTQIRFDKRTLHSNNLYQIFTYV
                     KNQAYRLSDSNDTVAGMLLYAKTDIDIQPNQVYQMHGNQISVKNLDLNLQFASIAEQL
                     DDIITSHFVSPPKRY"
     gene            complement(2771..4468)
                     /locus_tag="U1099_000082"
     CDS             complement(2771..4468)
                     /locus_tag="U1099_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4866435.1"
                     /translation="MDEQNQFNWVNFYKEFAWKLLDYKDNRAELVEKVKAIYTKTKIH
                     MPTLEKDNHLIDIDPFTIFGLFNKQIKEENRIKILTAIAELFNLQTEIPSSFESIPVL
                     MNQNATFYYFIGDRDEKDIDDLWELFIAALTYSKEPTAEKKEKFSHYFDLAINKKGNG
                     NSKITMALYWIAPDFFLNLDSRNKWYVYESGEIPTDVIKKLPKMKSKISADDYFYISD
                     SMHHYLNSAESKLKDFKELSSMAWKYSKQINQEEKERSAGAISPLADETDTGNEIGSE
                     EVVEDSYTKNHFLSEVYMTEEEYDQLTSILQMKKNIILQGAPGVGKTFVAERLAFSLM
                     GKPDLERVMTIQFHQSYSYEDFIMGFRPSTSGFELRRGAFYTFCKKAESDKSHDYFFI
                     IDEINRGNLSKIFGELFMLIENDKRGKSLQLLYSDEQFSIPENLYIIGMMNTADRSLA
                     LLDYALRRRFAFFDIKPGFSTAGFKDYQTNLKNEKFDRLISCIEKLNQAISTEEVLGD
                     SFCIGHSYFCGLSAEVLDQGLLEAIVEYELIPLLKEYWFDEPTKIHDWSSKLRSAIQ"
     gene            4669..5457
                     /locus_tag="U1099_000083"
     CDS             4669..5457
                     /locus_tag="U1099_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866436.1"
                     /translation="MAVLKYSKARLLVLLIATGLSCIGIYWLGKEQNRLLNEQCHALN
                     IRIINDLGAKIDAIGGPQNPRIIGFFQQDDTTAISQRIGTASEEELKIAKPDNLFQKE
                     WIVLYPQTRSSPFENTSAYAVMKTSIKADWLHVTTSSETELDIFYEKADESLLTLEDL
                     VQDKESFHATLKTILVSAKNEAEIQVQKDILEMFESDDWSAIPFAYTEKSLILEKAVI
                     SVSAFVDSLNPYYFSEQTLADLRLSEESRQALEDSVDKTIITYP"
     gene            5485..6432
                     /locus_tag="U1099_000084"
     CDS             5485..6432
                     /locus_tag="U1099_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866437.1"
                     /translation="MDVFPSFFIFLFNKLNIFVDLKNIFCYTCVMKTSTLGKLLELPN
                     FHHFYTVPIRENTESSGNYIIVFEQDKQPVFVDLKNETGHTREVRFASHFDSAGLLVG
                     LGAIPINYWPTCQILGVQKNNNRRGLSLSSLMDGYGRLPIFKEMAERYRARGQSTIYD
                     PYRYSYTIDEKKVIKDNQLTLIPKKEYEKIKDFHPFLQTKHGNVYAVPCFYQDRKRNQ
                     TIKIYIIGWYFYEKQLKISWKPSHSRKSRWRSSLVSVDKLSDRFFFQGELYKKGTYPV
                     FLEDLTIKNKAYQHRLDREFAAFLSIIEEQKRRAQLRVK"
     gene            complement(6453..6785)
                     /locus_tag="U1099_000085"
     CDS             complement(6453..6785)
                     /locus_tag="U1099_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEM4866438.1"
                     /translation="MILNVQDFHLAAFAYYDGLDVIDQLKPGTPVQLVGEPSNPHDSE
                     AVAIFYQGTKLGYIPSDKNSLISRLIYFGHGDILEARIQMSNTENHPDRQFRVVVKLK
                     DNRKNDSR"
     gene            complement(6782..7534)
                     /locus_tag="U1099_000086"
     CDS             complement(6782..7534)
                     /locus_tag="U1099_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 1 (IF-1)"
                     /protein_id="HEM4866439.1"
                     /translation="MKRKYLALALGLLTIMSLSACQTEKKGSSASTSTADTQVSNANT
                     STSSTIQREISLSSMDIDALMQGNYDSILGTWQNSQGNSLVFNSKGLVADSQSLLGRG
                     KITDGIFETGYVDATIGDVTLLMIPKGTQANTTETDTTDSTRDRMIVISQETTADVVD
                     VYYRLTNSATSGIDPLKNTETGVQLDSGPKTIDYANSILGENNWRVIEGNYTRTESIP
                     YNILEGDDNARYTIYQNGVIINADYQIVYQPQ"
     gene            complement(7551..8309)
                     /locus_tag="U1099_000087"
     CDS             complement(7551..8309)
                     /locus_tag="U1099_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEM4866440.1"
                     /translation="MKRNVLITGASSGIGKAIAYVFAANGDNIIITGRRLDKLEEIKQ
                     DIEKQYHVQVSVHSFDITNMEQTIVNCQKILEEVGQIHILVNNAGLALGLDKFQDYDL
                     TDMMTMIDTNIKGLLTVTRQLLPQMVENNEGHIINIGSTAGIYAYAGAAVYAATKSAV
                     KVLSDGIRIDTIDKNIKVTTLQPGIVETDFSQVRFHGDKERAATVYQGIDALQANDIA
                     SCTLFVANQPAHVQISDITIMATKQATGFTIHRD"
     gene            complement(8410..9009)
                     /locus_tag="U1099_000088"
     CDS             complement(8410..9009)
                     /locus_tag="U1099_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEM4866441.1"
                     /translation="MKKLIRTLILLLLIFLGYKFYQTYSAVKQVMTYQTMVQEVLAEN
                     DTPANEELVLAMIYTETKGQEADVMQSSESATGYANTITDSRESIRQGIIYLTENLRL
                     AEEKGVEVWTAVQAYNFGPAYIDYIAEHGGEHTLSLAKEYSRTVVAPSLGNTTEETYT
                     YFHPLALLNGGKLYVNGGNIYYARQVRFNMHLMRLINLF"
     gene            complement(9010..9984)
                     /locus_tag="U1099_000089"
     CDS             complement(9010..9984)
                     /locus_tag="U1099_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEM4866442.1"
                     /translation="MDIFVKKAIIHQFSPDDTELVLSDQLLTVSPKIEEYLRKKIERV
                     FSDEAKTGQFNPDNPFLDYLDGDLLTNSVKIANLWKEAFSISENLKTNDLIFIEFERN
                     GVEHFTFLRISLRENLAHVGLESGSPLKITQNNLPGAGSAPDEALIVNLQTRKYHLIE
                     KRIKHNGSFLNYFSDNLLQVTPAISAKKSIKAVEQTAQKIADNFHQGDFQFQSKIKSA
                     IFNNLEEDNELSPEKLADQLFDNNLTARLNFVDQLKEVIPDKISFDEIDSSRQLKKFE
                     NQKLSLSNGIELIVPNAIYEDAESVEFIQNDNGTYSILIKNIEDIKSK"
     gene            complement(9984..11243)
                     /locus_tag="U1099_000090"
     CDS             complement(9984..11243)
                     /locus_tag="U1099_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEM4866443.1"
                     /translation="MIFDKVNYKEFDKEVWEAIQAEEKRQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTECVDVVESLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMALIEPGDTVMGMDLAAGGHLTHGASVSFSGQTYNFVAYNVDEETGLLDYDAIL
                     KQAKEVQPKLIVAGASAYARTIDFAKFREIADAVGAKLMVDMAHIAGLVAAGLHPNPV
                     PHAHITTTTTHKTLRGPRGGLILTNDEELIKKINSAIFPGIQGGPLEHVIAAKAVSFK
                     EVLDPDFKDYAQKVIENSKAMAEVFLANPNFKVITGGTDNHLFLVDVTKVVENGKVAQ
                     HLLDEVNITLNKNSIPYEKLSPFKTSGIRIGSAAITARGFGVEEARKVAQLTIKALEN
                     AENEKALEEVRQEVRALTDQFPLYEGL"
     gene            complement(11267..11698)
                     /locus_tag="U1099_000091"
     CDS             complement(11267..11698)
                     /locus_tag="U1099_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866444.1"
                     /translation="MIRPIEKGDLVVIREINAQSLGYDCSLEQTERQFFRCTSTLGHI
                     LLVYIDDISGAVQGYIHAQVYESLYSDTGLNILGLAVLPGHQVQGIGASLLKAVEQIA
                     QKEGYHFIRLNSAESRLQAHLFYEKNGYHSDKMQKRFIKHI"
     gene            complement(11695..12303)
                     /locus_tag="U1099_000092"
     CDS             complement(11695..12303)
                     /locus_tag="U1099_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEM4866445.1"
                     /translation="MTMDKLRTILENGGAVILPTETVYGLFAQALNEDAVNRVYQLKQ
                     RPRDKAMNLNVSNLNDIYFFSQNTPFFLEKLYNRFMPGPLTIILKANENVPFWVNSGL
                     DTVGFRLPNHEQTLRLISETGPLIGPSANISGNESGKKFSDIQAQFSVDLPGIEDDQA
                     LTGIDSTILDLSGQKARILRQGAISREEIQKAIPEIEFEVVL"
     gene            complement(12290..13123)
                     /gene="prmC"
                     /locus_tag="U1099_000093"
     CDS             complement(12290..13123)
                     /gene="prmC"
                     /locus_tag="U1099_000093"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEM4866446.1"
                     /translation="MNYAQLFAAYEEQLVAIGEEAESLSFTFRGLKGLNFTEFLLLLR
                     QEVTPTDKEEIDAIFQQLSQHRPAQYIIGKADFHGLEFAVDERVLIPRPETEELVNLI
                     LQENSGAGLRILDIGTGSGAIAISLAKARSDWEVVAVDISKDALAVAQENARTNQVSV
                     HFLESDVFQAVTGQFDLIVSNPPYISPDDTDEVGLNVLASEPHLALFAEEDGMAIYRQ
                     IAEQAGAFLKEKGKLYFEIGYKQGQDLTDLLALHFPKKRIRVLKDQFGQDRKVIADDN
                     G"
     gene            complement(13123..14202)
                     /gene="prfA"
                     /locus_tag="U1099_000094"
     CDS             complement(13123..14202)
                     /gene="prfA"
                     /locus_tag="U1099_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEM4866447.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEAATR
                     DTVTAYREYKTVLQNMVDAEEMIKESSGDADLEEMAKEELKQAKADKEAYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEGQGWRFEVMEASYNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLIMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVVDALVLYDQTQKLEELNK"
     gene            complement(14483..15064)
                     /locus_tag="U1099_000095"
     CDS             complement(14483..15064)
                     /locus_tag="U1099_000095"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEM4866448.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     AFGVVSSRIGMRRDAVAIDDDMDIFGFIEKMEPRPYCVLIDEAQFLRRHHVYALARVV
                     DELDVPVMAFGLKNDFRNELFEGSQHLLLLADKLDEIKTICQYCSKKATMVLRTQDGK
                     PTYEGEQIQIGGNETYIPVCRKHYFSPEIKELP"
     gene            complement(15163..15387)
                     /locus_tag="U1099_000096"
     CDS             complement(15163..15387)
                     /locus_tag="U1099_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866449.1"
                     /translation="MAYKYRMILSFLLAGLCLYLVATVFAKSIWEGPLFLAFSFYSLI
                     YGCVMLYKWKPTAAKIIFQCIGEFLSLPWS"
     gene            complement(15449..16756)
                     /locus_tag="U1099_000097"
     CDS             complement(15449..16756)
                     /locus_tag="U1099_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEM4866450.1"
                     /translation="MFKKKATQIVSETSIKKSRKFLNRKTAGITGGILLTTFIGSQLM
                     TAELPKKDDLYGQQYVTVVKHLQEAGFKNIQGVELSDLEFGKIGESNLVELVSVDGED
                     WKEGRALKNIPITISYHVPKKDAVEFNLPASKNLADVEKELKDSGFKQVELTPVLLVE
                     EGNADKKDKIDRLQIGNHTYQSNHFYSTSLPVTLTYFDVRKDNIKLPENLAEAKTKPE
                     LEKQLKTAGFTDIKWTAVADKDKAKHEKIQKINLGGAEIQLPTKQEIISKKSTPIVIT
                     YYDFSSFAELPSSISTKTATDTKKLFTDGGFSQVSEVATETNEIAKNGQIIAVEIDGK
                     SFNEMNDTVIKKDSKVIIKYWNAEKAIAEKARKEEEERLAAEAQKVAEAQSQVQQFAA
                     TPSQNTYYPNCKAVRQAGAAPIYRGEPGYGSHLDRDGDGVGCE"
     gene            16891..17073
                     /locus_tag="U1099_000098"
     CDS             16891..17073
                     /locus_tag="U1099_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEM4866451.1"
                     /translation="MPFVRIDLFEGRTEEQKIALAREVTEVVSRNTNAPKEAIHVFIN
                     DMPEGTYYPQGEMKRK"
     gene            complement(17123..18955)
                     /gene="lepA"
                     /locus_tag="U1099_000099"
     CDS             complement(17123..18955)
                     /gene="lepA"
                     /locus_tag="U1099_000099"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEM4866452.1"
                     /translation="MNLEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPIINKIDLPAADPERVRQEIEDVI
                     GLDASEAVPTSAKAGIGIEEILEQIVEKVPAPTGDVEAPLQALIFDSVYDPYRGVILQ
                     VRIVNGVVKPGDTIQMMSNGKTFDVTEVGIFTPKAIGRDYLATGDVGYIAASIKTVAD
                     TRVGDTVTLAKNPASEPLAGYKQMNPMVFAGIYPIDSNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERIEREFNIDLIMTAPSVVYHVNMTDGEMID
                     VANPSEFPDPTKIATIEEPYVKAQIMVPQEYVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRSSKLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEDDKK"
     gene            complement(19051..19464)
                     /gene="ndk"
                     /locus_tag="U1099_000100"
     CDS             complement(19051..19464)
                     /gene="ndk"
                     /locus_tag="U1099_000100"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEM4866453.1"
                     /translation="MERTFFIIKPDALKRGLAGQILSRIERRGFQIRDLKMVTATEEL
                     ISQHYDHLTDKPFFPQLVQYMTSGPMIAGIIEGPEVIKSWRDMMGATNPVNALPGTIR
                     GDFATAPVEGIVANVVHGSDSAEAAEREIGLWLGK"
     gene            complement(19487..20074)
                     /locus_tag="U1099_000101"
     CDS             complement(19487..20074)
                     /locus_tag="U1099_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEM4866454.1"
                     /translation="MEINTHNAEILLSAVSKAQYPGDDIPEIALAGRSNVGKSSFINT
                     LLGRKNLARTSGKPGKTQQLNFYNIDDKIRFVDVPGYGYAKVSKTERAKWGKMIEEYL
                     TTRENLRVVVSLVDMRHEPSADDVQMYDFLKYYEIPVIIVATKADKIPQGKWNKHESM
                     IKKKLDFDKNDQFVIFSSETRHGYDQAWDAILSNI"
     gene            complement(20130..21356)
                     /gene="clpX"
                     /locus_tag="U1099_000102"
     CDS             complement(20130..21356)
                     /gene="clpX"
                     /locus_tag="U1099_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEM4866455.1"
                     /translation="MAVKHTHELIYCSFCGKNQEEVKKIIAGNNVFICNECVELAQEI
                     IREELAEEVLTDLADTPKPQELLNILNNYVIGQDRAKRALAVAVYNHYKRINFQDSRD
                     ENDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVENIL
                     LKLLQAADFNIDRAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIHVDTKNILFIVGGAFDGIEEIVKQRLGEKIIGFGHNNRAIDE
                     KESYMQHIIADDIQKFGIIPELIGRLPVFAALDQLTTEDLVRILTEPKNALVKQYQTL
                     LSYDDVELDFDEDALLAIAEKAIERKTGARGLRSIIEETMLDVMFEVPGQDNVKRVRV
                     TKAAVDGKEKPLLETA"
     gene            complement(21554..22066)
                     /locus_tag="U1099_000103"
     CDS             complement(21554..22066)
                     /locus_tag="U1099_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM4866456.1"
                     /translation="MTKKIVAIWAQDENGLIGKGDRLPWSLPADLAHFKETTIGHTMV
                     MGRITFDGMGKRALPNRHTIVLTTDKTYQLESERITILHSVEDVLDWYNKQEQTLFVI
                     GGGQIFTAFAPYIETLIVTDIHGQFNGDVYFPKAFPMEKFQLQSANLRPKDEKNPYDF
                     TIKTYERRDG"
     gene            complement(22404..23243)
                     /locus_tag="U1099_000104"
     CDS             complement(22404..23243)
                     /locus_tag="U1099_000104"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEM4866457.1"
                     /translation="MTKADIIFKENIRKIMEEGVFSENARPRYKDGNVANSKYVTGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEILWIYQDQTNDLSVLQEKYGVQYWNDWEVEGT
                     GTIGERYGAIVKKHDIISKILKQLEENPWNRRNVISLWDYEAFDQSAGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAEELLRRTPSEVEPRLVLNVPDGTNFFDIRAEDFELVDYNPVKPQLKFD
                     LAI"
     gene            complement(23296..23598)
                     /locus_tag="U1099_000105"
     CDS             complement(23296..23598)
                     /locus_tag="U1099_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866458.1"
                     /translation="MNLTLLKRLNLVLYGIAIFLFVMLFLPIGQWFDIVNVNFKLTFF
                     IIPFFGLASLPTAIYTKNVRQILLSAILVALYFILFSLITALSGLFHLNFYSFFFK"
     gene            complement(23610..23888)
                     /locus_tag="U1099_000106"
     CDS             complement(23610..23888)
                     /locus_tag="U1099_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866459.1"
                     /translation="MKQVNMSKIINYLTILGLLILLSAFFLDNWIRDWFFPSSWGNVA
                     TMLILPLLGTLILILSIYYKKLWTGLISIFLMISFPLIFGIGYFIFGP"
     gene            complement(24015..24974)
                     /locus_tag="U1099_000107"
     CDS             complement(24015..24974)
                     /locus_tag="U1099_000107"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEM4866460.1"
                     /translation="MSKKIIGIDLGGTSVKLAILTTEGEIQEKWSIKTNILDDGSHIV
                     PDIIDSIQYRFETHGLTKDNFLGIGMGSPGVVDSEAGTVIGAYNLNWKTLQLVKDQFE
                     SALGLPFFIDNDANVAALGEQWVGAGNNNPNVVFMTLGTGVGGGVIAAGNLIRGVKGA
                     GGELGHITVDFDEPFACTCGKKGCLETVASATGIVNLSRRYADQYAGDAKLKQMIDDG
                     QDVTAKDVFDLAKEGDDLALIVYRHFSEYLGVACANIAAVLNPAYIVLGGGVSAAGEL
                     LLDGVRKVFAENSFPQIKESTQIVLATRGNDAGVLGAASLVLK"
     gene            complement(25070..25405)
                     /locus_tag="U1099_000108"
     CDS             complement(25070..25405)
                     /locus_tag="U1099_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866461.1"
                     /translation="MKVLVMSYMVIYLLVTLGAALFSYLKTKKMNTLRLILTILSMIL
                     LTSTLYFYSQSYHDLQMVGFALGFTFISTLFLYNGTKEGSNFTTVMLFSIGRFILHIQ
                     FLILLYLFR"
     gene            complement(25406..27274)
                     /locus_tag="U1099_000109"
     CDS             complement(25406..27274)
                     /locus_tag="U1099_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM4866462.1"
                     /translation="MSDFIVEHLTKSVGDKTVFADLSFIIHQGDRIGIIGVNGTGKTT
                     LLDVLSGRIGFDGDVSPFRTKNAYKIAYLTQEPDFDESKTVLDTVLSSDLRETQLIRE
                     YELLLSHYDEASQARLEKVMAEMDSLNAWEIESQVKTVLSKLGLTDLNKTVVELSGGL
                     RRRVQLAQVLLGNADLLLLDEPTNHLDIDTIEWLTTFLKNTKKSVLFITHDRYFLDNV
                     ATRIFELDRASLTEYQGNYQDYVRLKAEQDERDAALRHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKGDLANKIDDSELEINFETSRIGKKVINFENVSFSYPDKPILAD
                     FNLLIQNKDRIGIVGDNGKGKSTLLNLIAGDLQADSGKVDIGETIRIGYFSQTIKGLD
                     ESKRVINFLQEVAEEAKTTSGMVSIAELLEQFLFPRNTHGTLIEKLSGGEKKRLYLLK
                     ILLQRPNVLLLDEPTNDLDIATLTVLEHFLQSFTGPVITVSHDRYFLDKVANKILAFE
                     DSGIETFFGNYTDYLDEKAFLASSSAISLEKPKEKTEKIKENKKRMSYFEKQEWATIE
                     EDIAGLEERIAAIEAEMLTCGSDFTKLSDLQKELDEKNDLLLEKYERYEYLSELEG"
     gene            complement(27267..28478)
                     /locus_tag="U1099_000110"
     CDS             complement(27267..28478)
                     /locus_tag="U1099_000110"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEM4866463.1"
                     /translation="MKLNNLPSEFQEALPILEKIKAAGYEAYFVGGSVRDAILGRPIH
                     DVDIATSSYPQETKQIFSRTIDVGIEHGTVLVLEGKKEYEITTFRTEEEYVDFRRPSQ
                     VSFVRSLEEDLKRRDFTVNAFALDEEAQIIDLFEGMTDLENRTLRAVGIPAERFNEDA
                     LRIMRGFRFAATLDFEIEPTTFEAMVKTAPLLEKISVERSFIEFDKLLMADFWRKGLR
                     AMIDSKAYNFLPDLAGKSDELETMLTSLTEEFRFSTSEQAWAMLFVCLGIDSIKSFLK
                     KWKTSNDFQRSVVKLVEIYQLRQAGPVTKQICFKYGKEFLYLVEELRQAQGLVTDFVA
                     IDKIDQALTIHDKHEIVVNGGHLMKAFDLKPGPVLGELLKEVEFQIVEGQLENEEQAI
                     MTFVKGILENE"
     gene            complement(28475..29242)
                     /locus_tag="U1099_000111"
     CDS             complement(28475..29242)
                     /locus_tag="U1099_000111"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEM4866464.1"
                     /translation="MTIKVIIAGFKGKMGSTAVEMVKGDAALSLAALVDPFATETEVD
                     GVPVFKTKEEVASLEADVWVDFTTPKFAYENTRFALENGFAPVVGTTGFTPEEIEELT
                     ALSAEKGLGGLIAPNFAIGAILLMQFAAQAAKYFPNLEIIELHHDKKKDAPSGTAVKT
                     AELISQVRQSQTQGAADEEELIAGARGAAFDGFRIHSVRLPGLVAHQEVIFGAQGEGL
                     TIRHDSYDRISFMGGVNLGIKEVVKRSQLVYGLEHLL"
     gene            complement(29251..30102)
                     /locus_tag="U1099_000112"
     CDS             complement(29251..30102)
                     /locus_tag="U1099_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM4866465.1"
                     /translation="MKLAVITDSSAILNVQSIERDDLFVLSIPVSIDGENYIEGQNLT
                     VEEFYQKMASSKELPKTSQPSLMELEEILAKLTEKGYTHALGLFLSSGISGFYQNIQY
                     LADEFEGLTVAFPDTKITSAPLGMMVENVLKWADAGLSFEEITGKLDQEIGKTTAFIM
                     VDDLNHLVKGGRLSNGAALLGNLLSIKPILYFTGEGKIEVYEKVRTEKKAIKRLLEIL
                     QEQTTEGQYQIAIIHANAPQKAENFKQQLEEAGVGSDLPIVSFGSVIGTHLGEGAVAF
                     GISPIIE"
     gene            complement(30104..30478)
                     /locus_tag="U1099_000113"
     CDS             complement(30104..30478)
                     /locus_tag="U1099_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEM4866466.1"
                     /translation="MEIIRDKEFVNQYHFDARNHAWEKENGIPETKLKVDFQLIEQNR
                     AENRTSMITILRFMIVLDHFVISGAMSQAVHLPNRLVEEPTEFTDEEKRVLVEPLLDI
                     LKRMTYEVTEIAFDAPGVNLEF"
     gene            complement(30574..32529)
                     /locus_tag="U1099_000114"
     CDS             complement(30574..32529)
                     /locus_tag="U1099_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM4866467.1"
                     /translation="MKIQDVLRKDVMLLDLQATSKEAVIDEMIASLVDKGYVTDFDVF
                     KTGIMNREAQTTTGLGDGIAMPHAKNAAVKEATVLFAKSNNGVDYASLDGQPTDLFFM
                     IAAPEGANDTHLAALAELSKYLMKAGFADRLRAATNPEEVIAVFDTAEAADKAVEEVV
                     AAPSGDRPFIVAVTACTTGIAHTYMAEEALKKQAAEMGVGIKVETNGASGVGNKLTAE
                     DIKNAAGVIIAADKAVDMPRFNGKPLVSRPVAAGIKQPEELINIILNGKAAAYTASEG
                     AATVESSEKLSLGKAFYKHLMSGVSQMLPFVIGGGILIALAFLFDGALGVPQDSLGSL
                     GSYHEIAAMFMKIGGAAFGFMLPLLAGYIAYSIAEKPGLVAGFVAGAIASSGLAFGKI
                     PYAAGGEETLALAGVSSGFLGALVGGFLAGGVVLVLRNALRNLPKSLQGLNAILLLPL
                     LGTAITGFLMFFVNIPMAAINTGMNNFLAGLEGSSAILLGLVLGGMMAVDMGGPVNKA
                     AYVFGTGTLAATVADGGSVAMAAVMAGGMVPPLAVFVATLLFKDKFTQEERNSGLTNI
                     VMGLSFITEGAIPFGAADPARAIPSFIAGSALAGALVGLSGIQLMAPHGGIFVIALTS
                     NPLLYIAYVLIGAVVSGVLYGALRKAK"
     gene            complement(32526..33437)
                     /gene="pfkB"
                     /locus_tag="U1099_000115"
     CDS             complement(32526..33437)
                     /gene="pfkB"
                     /locus_tag="U1099_000115"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEM4866468.1"
                     /translation="MIYTVTLNPAIDYIVRLEHVETGSVNRMESDDKYAGGKGINVSR
                     VLKRLGYPNTATGFLGGFTGQFIKDGLIAEGIDTDFVQVDQDTRINVKIKADQETEIN
                     GLGPIVTDTQLAELEMVLAGLTKEDTVVFAGSAPASLGNEVYNRLIPVAKKAGAQVVC
                     DFEGQTLLDSLAHQPLLVKPNNHELEAIFNVELNDIADIETYAKKILEMGAQNVIISM
                     AGDGALLVTPAATYFAKPIKGTVKNSVGAGDSMVAGFTGEYVRSQDPIEALKWGVACG
                     TATAFSDDLASIEFIKETYEKVEVETL"
     gene            complement(33434..34183)
                     /locus_tag="U1099_000116"
     CDS             complement(33434..34183)
                     /locus_tag="U1099_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM4866469.1"
                     /translation="MNILKSERKQIILERIHSQQFVRLEELIEVLDTSESTVRRDLDE
                     LENEGKLRRVHGGAEAPRNLQLEESILEKSVKNVQEKREIAERAAQQIVNGDVIFLDA
                     GTTTGVLIDYLQQENLTVVTNSIHHAVRLVERKIKTIIIGGFVKQSTDASVGAVALEQ
                     IRQLNFDKAFLGMNGIDKNYFTTPDIEEATIKRTIIDNAKESYVLADASKIGQFSFVK
                     VAAIEKSNIICQSSESSLLDIIKEKTRVIEV"
     gene            complement(34287..35342)
                     /locus_tag="U1099_000117"
     CDS             complement(34287..35342)
                     /locus_tag="U1099_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HEM4866470.1"
                     /translation="MKRKVFEHYQPYKIKQKARFDLGNNENRIIDWSFLPQETLENLD
                     VNQLSFYGDNTYSELIEKYAAYLGVNPKQVTVGVGSDHLIHMIVSTFMEKDDTFLTVN
                     PDFFMYETYNHMHGSRFETIDLEAKNDTLVLPVEKLLARAEEVKAKIIMLSNPNNPSS
                     VAYSLEDLEKLAISFKGLLVVDEAYVEFANVESFISRLQQFDNVLVLRTLSKAFGLAG
                     LRVGFAVGSEELIYELDKVIPPFSLSNLTAKMAAVALNYTDKVLETVGVIQNLREEMI
                     LFLQGLDNCQVLPSQASFVTFKAPWAEEFYQQALAQDWNFKYYPTGKLEGHIRLSIGR
                     PEEMDMMKKMIEKLVEA"
     gene            complement(35373..36098)
                     /gene="trmD"
                     /locus_tag="U1099_000118"
     CDS             complement(35373..36098)
                     /gene="trmD"
                     /locus_tag="U1099_000118"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEM4866471.1"
                     /translation="MRIDILTLFPEMFAPLEHSIVGKARDKGLLEINYHNFREKAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTMDSIEQAKPRVILLDPAGRTFNQAYAEELAQEEQ
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKEVSH
                     TDDSFSSGLLEYPQYTRPYEYRGMVVPDVLMSGHHENIRKWRLEESLRKTYQRRPDLL
                     ENYNFTAEELTIFEKIKAEDTVD"
     gene            complement(36088..36606)
                     /gene="rimM"
                     /locus_tag="U1099_000119"
     CDS             complement(36088..36606)
                     /gene="rimM"
                     /locus_tag="U1099_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEM4866472.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAKLALFDDK
                     DQFVMEVEIANHRKAKNFDIIKFKGMYHINDIEKYKGFSLKIAEENLTDLEDGEFYYH
                     EIIGLDVYENDILIGQIKEILQPGANDVWVVKRKGKKDLLLPYIPPVVLNIDIPNNRV
                     DVELLEGLDDEN"
     gene            complement(36704..38053)
                     /locus_tag="U1099_000120"
     CDS             complement(36704..38053)
                     /locus_tag="U1099_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEM4866473.1"
                     /translation="MYQTRNNKEKLWLFIKIFLPILIYQFANYSASFIDTMMTGQYST
                     MDLAGVSMATSLWNPLFSFLTGIVSALVPIIAQYLGKGERDKIREEFHQFVYLAIGLT
                     ILLLCSVLFIAVPALAQFGLDESIFQVGRQYLYFISIGILPLLLFSVCRSFFDALGLT
                     QLSMYLMLLLVPFNSLFNYLLIYGKMGLPALGGAGAGLGTALAYWAVLLVIVIVMCKN
                     KTIASYQIWQWSPIDLSLLKEGLKIGLPIGLQVFAEVAIFAVVGLYMAKFSAQIIAAH
                     QSAMNFATLLYAFPASVSSALAIVIAYEVGAKRPQDVKAYSRLGRLVALGFAVLTLTF
                     LYFFRSKVAYLYGNDAEFIRLTSHFLSFALMFQLADAFTAPIQGILRGYKDTTVPFIL
                     GLVAYWSMTFPVAFLLERFFQMGPEAYWIGLISGIFVCGIALNLRLIIIAHSHLSVK"
     gene            complement(38123..39106)
                     /gene="guaC"
                     /locus_tag="U1099_000121"
     CDS             complement(38123..39106)
                     /gene="guaC"
                     /locus_tag="U1099_000121"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEM4866474.1"
                     /translation="MFNETPVFDYEDIQLIPNKCIINSRSEADTTVTLGKYSFKLPVV
                     PANMQTIIDEDVAEMLAKDGYFYIMHRFDEAGRIPFIKRMHEQGLIASISVGVKEYEY
                     EFVTSLKADAPEFITIDIAHGHAESVIKMIQHIKKELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTIEVDGEKFKEYYGSASEYQKGAYKNVEGKKIL
                     LPAKGHLKDTLVEMEQDLQSSISYAGGRDITSLKHVDYVIVKNSIWNGDSI"
     gene            complement(39392..40015)
                     /gene="nth"
                     /locus_tag="U1099_000122"
     CDS             complement(39392..40015)
                     /gene="nth"
                     /locus_tag="U1099_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEM4866475.1"
                     /translation="MVLSKKRARKVIEEIIALYPDAKPSLDFRNHFELVCAVLLSAQT
                     TDAAVNKATPGLFEAFPTPQAMAAAQVKDIEPYISRLGLYRNKAKFLKECAQQLLDRH
                     DGIVPQTREELEALAGVGRKTANVVLSVGFGIPAFAVDTHVGRICKHHDIVKKSATPL
                     ETEKRVMEVLPPELWLPAHQAMIYFGREVCHPKNPECEKFPQLYEFD"
     gene            40113..40964
                     /locus_tag="U1099_000123"
     CDS             40113..40964
                     /locus_tag="U1099_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEM4866476.1"
                     /translation="MDLQELCRKVIQARPRNSHKGSYGRVLLIGGLYPYGGAIIMAAL
                     ATVNSGAGLVTVATEKDNIPALHSHLPEAMAFSFDDQALLISSLENADLVLIGPGFGE
                     NSRAEQLLDFVFEHLSDQQILVLDGSALTLVAKQNRKNFPCKQVILTPHQKEWERLSS
                     IPIADQTSQRNLEALQSFQENTILVAKSSATQILKTQPNQVTPIGAGGPYQATGGMGD
                     TLAGMIAGFAVQFPHVQLYERILVATYLHSYIADQLAQDYYLVKPTDVSRNIQKTMLQ
                     FQTQTTA"
BASE COUNT        11800 a         8830 c         7889 g        12520 t
ORIGIN      
        1 aaagggaaaa acatgacaaa acaagatgtc caaaaaatta ttgtattgga ttatggtagc
       61 cagtacaacc aacttatttc acgtcgaatt cgtgaatttg gtgtcttttc agaattgaaa
      121 aaccataaaa ttacagctga ggaagttcgt gctatcaatc caattggtat cgttctttca
      181 ggtggaccaa actctgtcta cgcagaaaat gcctttgata ttgatccaga aatctttgaa
      241 ttgggtattc ctatcttagg gatttgctat ggtatgcagt taattaccca taaattggga
      301 ggtaaggttg ttcctgctgg tgaggctgga aatcgtgaat atggtcaatc aaatcttcaa
      361 ctgaagactg aatcagcgct ttttgctgga acaccagaag aacagcttgt actcatgagc
      421 cacggggatg ctgtaactga aattcctgct gatttccacc tggttggtct gtctgctgac
      481 tgcccttacg ctgccattga aaacacggaa cgccgtatct acggtatcca gttccaccca
      541 gaagttcgtc attccgtata tggaaacgat attttgaaaa actttgcgtt tggtatctgt
      601 ggagccaaag gcgactggac aatggaaaac ttcatcgaaa cagaaattga aaaaatccgc
      661 cagactgttg gggacaagaa agtcttgctc ggtttatcag gcggtgtgga ttcatctgtt
      721 gttggtgtgc ttcttcaacg tgctattggt gaccaattga cctgtatctt cgttgaccac
      781 ggtcttcttc gtaagaatga aggcgatcaa gttatggaaa tgttgggcgg taaatttggc
      841 ctcaatatta ttcgcgtcga tgctgctaag cgtttcttgg atctacttgc tggcgtatct
      901 gaccctgaga agaaacgtaa aatcatcggt aatgaatttg tttacgtatt tgacgatgaa
      961 gcgagcaagt tgactgatgt tgaattccta gcgcaaggaa cactttatac cgatattatc
     1021 gagtcaggaa cggatactgc tgagacaatc aaatctcacc acaacgttgg tggtctgcca
     1081 gaagatatgc agttcaagtt gattgaaccg ctcaatactc tctttaagga tgaagttcgt
     1141 gctctcggta ctgctcttgg tatgccggat gaagttgtat ggcgccaacc attcccagga
     1201 ccaggccttg ccattcgtgt catgggtgaa atcactgagg aaaaacttca aactgttcgc
     1261 gaatctgacg ctatcttgcg tgaggaaatt gcaaaagcag gtcttgaccg cgatgtgtgg
     1321 caatatttca cggttaatac tggcgttcgc tccgtaggtg tcatgggaga tggtcgtacc
     1381 tacgactaca ccatcgccat ccgtgctatc acttctgtcg atggaatgac agctgacttt
     1441 gccaaactac catgggatgt tcttcaaaaa atctctgtcc gtatcgtaaa cgaagtcgac
     1501 cacgtcaacc gcatcgtcta tgatataaca agtaaaccac cagcaacagt tgagtgggag
     1561 taaattagtc cagtggacta atttacccga tgcctaaaaa taaaaaagca tcgctgatct
     1621 gggaaatcaa ttaaccagac gctccaaaat ataaaaaaac aatcaaagag tagtttagtg
     1681 ctactacttt gattgttttt ttgttttcta gcttaaagct ctaactagta ccgttttgga
     1741 ggggatacaa aatgcgatgt aatgatgtca tccagttgtt cagcaatact tgcaaactgc
     1801 aggtttaagt ctaaattttt gacactaatc tggtttccat gcatttggta gacctggtta
     1861 ggttgaatat ctatatcggt tttagcgtat agaagcattc ctgctactgt atcgtttgaa
     1921 tctgacaggc gataagcctg gtttttcaca taggtaaaaa tttggtatag attatttgaa
     1981 tggagtgtgc gcttatcaaa gcgaatctgt gtgttactag aataatattt agcatcaata
     2041 atcaatatag tatctttata tttgaggtaa atatcactct gcatgatggg gagcatttct
     2101 ccaaaaccat catctagtgc ccaaggtatc tgagaggcgg taacctgtat ttggggataa
     2161 tgctttttat aatatccaag aatgaacttc tcatatagca aggattccct ccgctcatca
     2221 agaaaattca tcaatttctt attcccctcc ctctctgtat gaattaatcc attcaccacc
     2281 aaatagcaga ttgacattag taattgatac gactgattgt ttcgattata ttgtaaattc
     2341 caattgattg agtgcagatt aatttttgag acttctccaa agaaaactaa gagttttctc
     2401 aatttctttt tcctgtccct gcttatatca gcttttagca gtatctcaat gcttgctttg
     2461 ataatgcgat tcattctact gtcaagagaa aaatcatcgt attggcaaac aagttgcttt
     2521 ctacgccaat tcggaggtgt taaagattca gagatattta ttttaccttt aagtgccgag
     2581 agtgactcgg tttgagaacg atactctcga ccaagtcctc ttttcacctg tatcgagagg
     2641 ctgattatca ttatttccgc cagtaaatct gcagcatttt taaattcttc agtagcgatc
     2701 cgtttatatt cctgcttatg aaggattttg aatgcgtagg atagcatata gtagatattt
     2761 tggacaggta tcactggatg gcactcctta acttactact ccaatcatga atttttgtcg
     2821 gttcatcaaa ccaatattct ttcaagaggg gaatcagttc atattcaaca atggcttcta
     2881 gcaaaccttg gtccagtact tctgccgata aaccgcagaa gtaactatgc ccaatacaga
     2941 agctatctcc cagtacttcc tctgttgaaa tggcctgatt tagcttttct atacaggaaa
     3001 tgagtctatc gaatttttcg tttttcagat ttgtttgata atctttgaat cccgcagttg
     3061 aaaaacctgg cttgatgtca aagaaggcaa agcgtcgtct cagtgcataa tcaagtaggg
     3121 ctaaactcct atctgcggta ttcatcatgc cgatgatata tagattttct ggaattgaga
     3181 attgctcatc cgagtagagg agctgtaaag acttgcctcg tttgtcattt tctatcagca
     3241 taaacaattc accaaaaatt ttgctcaaat ttcccctgtt tatttcatcg ataataaaga
     3301 aataatcatg actcttgtca gactctgctt ttttgcagaa agtatagaat gcccctcttc
     3361 tcagctcaaa gccacttgtc gaaggtctaa agcccatgat gaagtcctcg taggaatagc
     3421 tttggtgaaa ctgaattgtc atgacccttt ctaaatctgg tttacccatt aatgaaaatg
     3481 ctaggcgttc tgcaacaaac gtctttccaa ctcctggcgc tccttgtaag atgatattct
     3541 ttttcatttg gagtatggag gttaattgat catactcttc ttctgtcata tagacttctg
     3601 ataggaagtg atttttcgta tagctatctt ctactacctc ttcactacct atctcgtttc
     3661 cagtatcagt ttcatcagcc agtggagaaa ttgctccagc acttctttcc ttctcttctt
     3721 gattgatctg tttagagtat ttccaagcca tggatgataa ttctttaaaa tctttcagtt
     3781 tgctctctgc actgtttaaa taatgatgca tagagtccga gatataaaaa taatcatcgg
     3841 cagaaatttt tgacttcatc tttggtagtt ttttgatgac atcagtagga atctccccag
     3901 attcgtatac ataccactta tttctgctat ctaagttcaa gaaaaagtca ggagcaatcc
     3961 aatacaaggc catcgtaatc ttgctgttgc catttccttt cttgtttata gccaaatcaa
     4021 aatagtgtga aaatttttct ttcttctcag ccgtaggctc cttgctatag gtcaaagcag
     4081 caatgaaaag ttcccataag tcatcaatat ctttctcatc acgatctccg ataaaataat
     4141 agaaagtcgc attctgattc atcagtactg gtatactttc aaaagaggat gggatttccg
     4201 tttggaggtt gaataattct gctatagctg tcagaatttt aatgcgattc tcctctttta
     4261 tttgcttgtt gaagagtcca aatatcgtaa aaggatcaat atctatcagg tgattgtcct
     4321 tttctaacgt gggcatatgt atctttgttt tggtatagat agcttttact ttctcaacca
     4381 gctctgctct attatcttta tagtctagta atttccaagc aaattcctta taaaagttta
     4441 cccaattgaa ttgattttgt tcatccatag tatcctccaa cggtgttgta tctacaccta
     4501 ttaaattatg agttcgaatt tacctatatt ttatcattaa acattcctgt cgtaaactta
     4561 gaatttatat acttgtgtaa cagtttaatg tggtacctta aaataaaata gtttcttttg
     4621 atatttatat atgataatat atgactgaaa gcgttttaga aaggtgtgat ggctgtgttg
     4681 aaatactcga aagcgcggtt acttgtcttg cttattgcca ctggtctatc ttgtatcgga
     4741 atctactggc tcggaaaaga gcaaaataga ctattaaatg agcagtgtca tgccttgaac
     4801 atacgcatta ttaatgatct gggagcaaaa attgatgcca ttggcggtcc gcaaaatccg
     4861 cgtatcattg gattctttca gcaagatgac actactgcta tcagccagcg gattggtacc
     4921 gcttctgagg aggaactgaa aatagctaaa cctgacaatc tattccagaa agagtggatt
     4981 gttctctatc ctcaaacgcg cagcagtccc tttgaaaata cttctgccta tgcagtaatg
     5041 aaaaccagta tcaaggctga ctggctccat gtgactactt cgtcagagac ggaattagat
     5101 attttttatg agaaggcaga tgaaagcttg ctgaccttgg aagacttggt gcaagacaag
     5161 gaaagttttc atgctacact aaaaacaatc ctagtttcag ctaaaaatga ggctgaaatt
     5221 caagttcaaa aagatatttt agaaatgttt gagagtgatg attggtctgc tattcccttt
     5281 gcttacactg aaaaatctct gatattggag aaagctgtca tttcagtttc tgcatttgta
     5341 gatagtctaa atccctacta tttttcagaa caaacccttg cagatttgcg tttatcagaa
     5401 gaatcgcgcc aggctttaga agattcggta gataagacaa ttatcaccta tccttgaata
     5461 aacggtggga gtgggaaagt cgtcatggat gtctttccca gcttctttat atttttgttt
     5521 aacaaattaa acatttttgt ggatttgaaa aatatttttt gttatacttg tgttatgaaa
     5581 acatcaacac ttggaaagtt actagaatta ccaaattttc accacttcta caccgttcca
     5641 attagagaaa atacggagtc ttctggaaat tatataattg tttttgaaca ggacaagcaa
     5701 cctgtatttg ttgatttgaa aaatgagact ggtcacactc gtgaggtacg ctttgccagt
     5761 cattttgaca gcgccggtct tctggtcggc ttaggagcca ttcctatcaa ttattggcct
     5821 acctgccaga ttcttggtgt gcagaaaaat aataatcgac gaggacttag tctatctagt
     5881 ctgatggatg gttatggacg tctacctatt tttaaggaaa tggcagagcg ttatagagcg
     5941 cgtggacaat caaccattta tgacccctac cgttattcat atactattga tgaaaagaag
     6001 gttattaagg ataatcaatt aacccttatt ccaaagaaag aatacgaaaa gataaaagat
     6061 tttcatcctt ttttgcaaac aaagcatgga aatgtttatg cagttccttg tttctatcaa
     6121 gacagaaaac gaaatcagac tattaaaatc tatattatag gctggtactt ttatgagaag
     6181 caattaaaaa tatcttggaa accttctcac tctcgtaaaa gccgctggcg ttcttctctt
     6241 gtttcagttg ataaattatc ggaccgcttc ttctttcagg gagaattgta taaaaaagga
     6301 acctatccag tgttcctgga agatctcaca ataaaaaata aggcctacca acatcgcctg
     6361 gatagggagt ttgcagcctt tttaagtatt attgaagaac aaaaacggag agcacagctc
     6421 cgtgttaagt agaaaatggg taggttggta aattaccgac tatcattttt acgattatct
     6481 tttagtttta caacgactcg aaactggcga tcaggatgat tttctgtatt ggacatctga
     6541 atacgtgctt ctagaatatc gccatgtcca aaatagatta accgactgat tagtgagttt
     6601 ttatccgatg ggatatatcc taactttgtg ccctgataaa agattgctac agcttctgag
     6661 tcatgtgggt ttgaaggctc tccgactagc tgaacaggtg ttcctggctt gagttggtcg
     6721 attacatcta gtccatcata gtaggcaaag gctgccaaat gaaaatcttg aacattgaga
     6781 atcattgtgg ttgataaaca atttgataat ctgcattgat aataacaccg ttttggtaaa
     6841 tagtgtaacg ggcattatca tcgccttcca aaatattata aggaatagat tcggtgcgcg
     6901 tgtagttacc ttcaatgacg cgccaattgt tttcgcctaa aattgagttg gcataatcaa
     6961 tcgtttttgg tccactatcc aactggacgc ccgtttcagt attttttaat gggtctatac
     7021 cgctagtggc tgaattggtc aagcgataat agacatctac cacgtctgct gttgtttctt
     7081 ggcttattac aatcattcta tcgcgcgtag agtcagtagt gtctgtctca gttgtatttg
     7141 cttgtgttcc tttgggaatc attaatagtg ttacatcgcc aatcgttgcg tctacatagc
     7201 ctgtttcaaa aattccgtca gttatttttc cgcggccaag taaggactgg ctatctgcta
     7261 ctaatccttt actattgaag actagtgaat ttccttggct attttgccag gtgcctagta
     7321 tactatcgta attgccttgc attagagcat ctatatccat agaactaaga gaaatctcac
     7381 gttggattgt tgatgacgtg cttgtattgg cattagaaac ctgagtatca gcagttgagg
     7441 tacttgcgga actgcccttc ttttcagttt gacaggcaga aagcgacatg atagtgagca
     7501 agccaagagc gagtgctaaa tattttcttt tcattctagc tatctcccct ctaatctcta
     7561 tgaatagtaa agccagttgc ctgtttggtt gccataatgg ttatatcaga aatctgcacg
     7621 tgtgcaggtt gatttgcgac aaatagagta cagctggcaa tatcatttgc ttgaagagca
     7681 tcaattcctt gatagactgt cgctgctctc tctttatctc catgaaaacg gacttggcta
     7741 aaatcagtct caacgatacc gggttgtaac gtagttacct tgatattttt atcaatcgtg
     7801 tcaatacgga tcccgtctga aagcaccttg acagctgatt tggttgcagc atagacagca
     7861 gctcctgcat aggcgtaaat accggccgtt gagccaatat tgataatatg accctcattg
     7921 ttttcaacca tttgtggcaa taactgacgt gtcacagtta gtaatccttt aatattggta
     7981 tcaatcattg tcatcatgtc agtcaaatca tagtcttgga atttgtctaa tcccaatgcc
     8041 agaccagcat tgtttaccaa aatatgaatt tgtccaactt cttctaagat tttttgacaa
     8101 tttacaattg tttgttccat attcgtaata tcaaatgaat gaactgatac ctgaacatgg
     8161 tattgctttt caatatcttg cttaatttct tctagtttgt ccaatcttcg tcctgtaatg
     8221 atgatattat caccattagc agcaaacaca taagcgattg ctttgccaat accactactt
     8281 gcacctgtaa tcaatacatt tcgtttcatc actctgtctc ctattctctt tattatacta
     8341 caattttacc tagtttgtaa aaatgctgta gttaaaaact catcagattt ccgatgagtt
     8401 tgataggtat taaaataaat taatgaggcg catcagatgc atattaaaac gaacctgtct
     8461 agcatagtag atatttccac catttacata taacttccca ccatttagca aagccaaggg
     8521 gtgaaaatag gtatatgttt cttctgtagt attccctaag cttggtgcta ctactgttct
     8581 ggagtattct ttagctaaag agagtgtatg ttctccacca tgctcagcaa tatagtcaat
     8641 ataggctgga ccgaaattat atgcctgaac tgctgtccaa acctctactc ctttttcctc
     8701 agccaaccgt aggttttctg ttaggtagat aattccttgc ctgatactct ctctactatc
     8761 cgtgatagtg ttagcatagc cagtagcgga ctcacttgat tgcatgacat ctgcttcttg
     8821 tcctttggtt tctgtgtaaa tcattgctag gactagttct tcattagctg gtgtgtcgtt
     8881 ttctgccaat acttcttgta ccatagtttg ataggtcatc acttgcttga ctgcactgta
     8941 ggtttgatag aatttgtaac cgagaaaaat cagtaaaaga agtatcagcg tacgtatcag
     9001 ttttttcatt tacttgctct ttatgtcttc aatatttttg ataagaatag aataggttcc
     9061 gttgtcattt tgaataaact caaccgattc tgcatcctca tagattgcat ttggcacaat
     9121 caactcaatt ccgtttgata aagatagctt ctgattttca aatttcttaa gttgacggct
     9181 actatcaatt tcatcgaagg aaatcttatc aggtatgacc tctttcagtt gatcgacgaa
     9241 atttaaacga gcggtcaggt tgttatcgaa taactgatca gccaattttt cgggtgaaag
     9301 ctcattatct tcttctaaat tgttgaaaat agctgacttg attttcgatt gaaattgaaa
     9361 atcgccttga tggaaattgt cagcaatctt ctgtgcagtt tgctctactg ctttgattga
     9421 tttttttgca gaaatagctg gtgtaacttg cagaagatta tctgagaaat aattgaggaa
     9481 ggatccattg tgtttgattc ttttctcaat caaatgatac ttccgcgttt gaagattgac
     9541 aatcaaggct tcatcaggtg ctgaacctgc tccaggtaaa ttattctggg tgatttttaa
     9601 aggacttccg ctttccagac caacgtgggc gagattttca cgcagtgaga ttctcaaaaa
     9661 ggtaaaatgt tccacaccat ttcgttcaaa ctctatgaaa atgaggtcat tggtttttaa
     9721 attctcagaa attgaaaatg cttctttcca tagattagca atcttgacag agttggtcag
     9781 taggtcacca tctaaatagt caagaaaagg attgtcgggg ttaaattgtc ctgtttttgc
     9841 ttcatcagaa aagacacgtt caatcttttt acgcaaatat tcttctattt ttgggctaac
     9901 agtcaaaagc tggtcggaaa ggaccagctc tgtatcatca ggactaaatt gatgtatgat
     9961 ggcttttttt acaaatatat ccattataaa ccttcgtaaa gtgggaattg atctgtcaaa
    10021 gcacgcactt cttgacggac ttcctcaaga gctttttcat tttctgcatt ttcaagtgct
    10081 ttgatagtta gttgtgcaac tttgcgagct tcctctactc caaaaccgcg agcagtaatg
    10141 gccgcagagc caatacggat accgcttgtt ttgaatggtg agagtttttc gtaaggaatt
    10201 gagtttttat tgagagtaat atttacttca tccaagagat gctgagcaac tttaccattt
    10261 tcaacaactt tggtcacgtc aacgaggaag agatggttgt ctgttcctcc tgtgatgact
    10321 ttaaagttag ggtttgcaag gaagacctca gccatggcct tgctgttttc aatcactttt
    10381 tgagcatagt ctttgaaatc tgggtccaaa acttctttga aagatacggc ttttgcagca
    10441 ataacatgct ctaatggacc accttggatg cctgggaaga tggctgagtt gattttcttg
    10501 ataagctctt catcattggt caaaattaaa ccaccacgtg gcccacgaag agttttatga
    10561 gtcgtagttg ttgtaatatg ggcatgtggc actgggttcg gatgaagacc tgctgctaca
    10621 agaccagcaa tatgagccat atccaccatc agtttggcac ccactgcatc tgcaatttcg
    10681 cggaatttag caaagtcaat tgtacgtgca taggctgaag cacctgctac aatcaatttt
    10741 ggctgtactt cttttgcttg ttttaaaatt gcatcgtaat caagcaagcc agtttcttcg
    10801 tcaacattgt aagctacaaa gttgtaagtt tgtcctgaga agctaactga tgcaccgtga
    10861 gtcaagtgac cacctgcagc taagtccatc cccataacag tgtctcctgg ctcaatcaag
    10921 gccatgtagg cagcacaatt ggcctggctt cctgagtgtg gctgaacgtt agcaaatttc
    10981 gcaccgaata tttctttagc acgttcaata gctaaacttt caactacgtc aacgcattcc
    11041 gtaccgccat agtaacgacg acctgggtaa ccttctgcgt atttattggt caaaatagaa
    11101 ccttgagctg ccataacagc tttagaaaca acgttttcag acgcaatcaa ttcgatatta
    11161 ttttgttggc gtttttcttc agcttggata gcttcccaaa cttctttatc aaattctttg
    11221 tagttaactt tgtcaaaaat catatacggt actcctttgt attgggctaa atgtgcttga
    11281 taaaacgctt ctgcatttta tcggagtgat agccattctt ttcgtagaaa agatgggctt
    11341 ggagacgact ttcagcagaa ttgaggcgaa taaaatggta gccctctttc tgtgcaattt
    11401 gctcaacagc tttcagcagg cttgctccaa tcccttgtac ttgatgacca ggcaaaacag
    11461 ctagtccgag aatattgagg cctgtatccg aatagagact ttcatagact tgtgcgtgaa
    11521 tgtaaccttg cactgcgcca cttatgtcat ctatatagac caacaagata tgccccaatg
    11581 tactcgtaca tctaaaaaat tgtcgctcag tttgttccaa tgaacaatca taccctaagg
    11641 attgagcatt gatttctcgg atgactacca agtcaccttt ctcaatggga cgaatcataa
    11701 aactacctca aattctattt cgggaatggc tttctgaatc tcttcacggc tgatagcacc
    11761 ttggcgcaaa atccgcgctt tctgtccaga aaggtccaaa atcgttgaat caatgcctgt
    11821 cagagcttgg tcatcctcaa tgccaggcaa atcaactgaa aattgtgcct ggatgtccga
    11881 aaacttcttg ccactttcgt ttcctgaaat attggcggac ggtccaataa gtgggccagt
    11941 ctctgaaatc aaacgtaagg tttgttcgtg attgggaaga cgaaatccaa cagtatccag
    12001 tccagaatta acccaaaaag ggacgttttc gttcgctttt aggataatcg ttagtggtcc
    12061 tggcataaat ctattataca atttctccag aaaaaatggt gtattttgac tgaaaaaata
    12121 aatatcgttc agatttgaga catttaagtt cattgccttg tccctcggac gctgtttcaa
    12181 ctgatagaca cgattgactg catcttcatt caaggcttgg gcaaaaagcc catagactgt
    12241 ttcagttgga aggataacag caccaccatt ttcaagaatt gttcggagtt tatccattgt
    12301 catccgctat gacctttcta tcctgtccaa attggtcttt gaggactcta attcgctttt
    12361 tggggaaatg cagagcgagt aagtcagtta ggtcttgccc ttgcttgtag ccgatttcaa
    12421 agtagagttt tcctttttcc ttcaaaaatg cccctgcttg ctccgcaatt tgtcgataga
    12481 tagccatgcc atcttcttcc gcaaataagg ccagatgagg ctcagaagcc aatacattga
    12541 gtccaacttc gtctgtgtca tctggtgaga tatagggcgg attagaaaca atgaggtcaa
    12601 attgccctgt cactgcttgg aagacatccg attctagaaa gtgaacagag acttggttgg
    12661 ttcttgcatt ttcctgagcc actgctaggg catcctttga aatgtcaact gctaccactt
    12721 cccaatcaga tctagctttt gcaagggaaa tagctatggc accgcttcct gtaccgatgt
    12781 ctaagatacg caaacccgct ccgctatttt cctgcaaaat caaattaact aattcttccg
    12841 tttcaggtct tggaatcaaa actcgctcat ccaccgcaaa ctccaagcca tgaaaatcag
    12901 ccttgccgat aatgtactgg gcaggtcggt gttgggaaag ttgctgaaaa atcgcatcaa
    12961 tctcttcttt gtcagtcggt gtaacttcct gacggagcag gaggagaaat tccgtaaagt
    13021 tcaatccctt taagccacga aaggtgaagg acagggattc tgcctcttca ccgattgcga
    13081 ctaattgttc ttcgtatgct gcaaataatt gagcgtaatt cattatttgt taagctcttc
    13141 tagtttttgc gtttgatcgt agagaacaag ggcatctacg acttcgtcca tcttacctga
    13201 caagatggta tcgagctttt gcaaggttaa gccgatacgg tggtctgtca cacgattttg
    13261 tgggaaattg taagtacgga tacgctctga gcggtcacct gtaccgatag ttgatttacg
    13321 ctctgcatcc tgctcatctt gggcaatctg tgcaaagtgg tcagctacac gggcacggat
    13381 aatcttcata gccttttcac ggtttttctg ctgggtccgt tcttcctgca tttctacctt
    13441 gatgttggtt ggcaagtgaa cgatacgcac ggcagttgca accttgttga cgttctgtcc
    13501 accagcacca gaagcgtggt aaatatcgac acgaagatct tttggatcga tgtcatattc
    13561 cacttcttca acttctggca tgatgaggac agttgctgtt gaggtgtgga cacgaccttg
    13621 gctttctgtc acagggacac gttggacacg gtgagctcct gattcatact tgagttttga
    13681 ataaacggat tgaccagaga ccatggcaac cacttccttg ataccgccga caccattata
    13741 agaagcctcc atgacttcaa aacgccagcc ttgaccttct gcatactttt gatacatggt
    13801 caaaaggtct ccagcaaaga gggcggcttc atcaccacct gcggcaccac ggatttccaa
    13861 gataatgttt ttgtcatcgt tagggtcttt tggtaaaaga aggattttca gcttttcttc
    13921 gtaagcctcc ttatcagcct tagcttgttt gagttcttcc ttagccattt cctccaaatc
    13981 agcatcgcca gaggattctt taatcatctc ttctgcatcg accatgtttt gaagtacagt
    14041 cttatattcg cggtaggctg ttactgtatc acgagttgca gcttcttcct tagacaactc
    14101 cataaaacgc ttggtatcgc tgactacatc agggtcagac aacaattcac caagttcctc
    14161 gtagcggtcc tccaccgcct gtaattgatc gtagatgttc ataattttaa gatattaagg
    14221 gcgttaaacg aacaaagcaa aaataggagt tttgacgaag gacgaaagcg tcctaggaaa
    14281 aactatcttt tttgcacagt tcgtagcccg tattcaattc ctttctaaat atttgacaga
    14341 acacacttct agttagcttg tagggcgtgt tcaattcctt tgccgtagta tgtaagtcat
    14401 gaaaattggc acagagtcat aggcaaattc agttctatac agttgctcaa tttacagtcg
    14461 cttctcattt cattttcaaa aattagggta attctttaat ttctggtgaa aaataatgtt
    14521 tgcgacagac aggaatgtag gtttcattgc caccaatttg gatttgttct ccttcataag
    14581 taggttttcc atcctgtgtt ctcaaaacca ttgtcgcttt tttagaacaa tattggcaga
    14641 ttgttttgat ttcatctaat ttatcagcca ataagagcaa atgttgggaa ccttcaaata
    14701 gttcatttcg gaaatcattt ttaagaccga aagccataac gggtacatct aattcatcga
    14761 ctacgcgagc aagtgcgtag acatgatgac gtcgaagaaa ttgtgcttca tcaatgagaa
    14821 cacagtaagg acgcggttcc attttttcaa taaaaccaaa gatatccata tcatcgtcaa
    14881 tcgccactgc atctcttctc atgccaatac gactggatac aacaccaaag gcatcacgag
    14941 tatcaagtgc agatgtcatg ataacaacag gttttccttg ttcttcatag ttgtgggcaa
    15001 ccttcaaaat ctcaatggtt tttcctgagt tcattgtccc atatttataa tataattgag
    15061 ccacttttct ctttccaatc ttaaattttc actccttcca ttctatcata aattgaccat
    15121 ttaggaaata gaaaaacaag cccgttttga gcttgtttga tattaagacc atggtaaaga
    15181 gagaaattct ccaatacatt ggaaaatgat tttagctgcc gttggtttcc acttgtagag
    15241 cataacacaa ccatatatca ggctgtaaaa agaaaatgca agaaacaacg gtccttccca
    15301 gatactcttg gcaaaaacag ttgctactag atacaaacat aatccagcga gtaggaacga
    15361 taaaatcata cggtatttat aggccatgag taatccctcc ttataagagc agtatagcat
    15421 gcaatgtaaa cgttgtcaat gttttttctt actcgcatcc aactccatct ccatcacggt
    15481 ctaaatgact accgtaacct ggttctccac gatagattgg tgcagctcct gcttgtctaa
    15541 ctgccttaca atttggataa tatgtgtttt gagatggtgt agcagcaaat tgctgaactt
    15601 gactttgtgc ttctgctact ttttgcgctt cggcagcgag gcgttcttcc tcctctttac
    15661 gtgctttctc agcaatagct ttttcagcat tccaatattt gataatgact ttgctatctt
    15721 ttttgatgac cgtatcgttc atctcgttaa aagattttcc atcaatctcg accgcaatga
    15781 tttgtccgtt tttagcaatt tcattggtct ctgttgctac ttcagatact tgagaaaaac
    15841 caccatcggt aaacaatttt ttagtatctg tagctgtttt cgttgaaatg gatgatggca
    15901 gttcagcgaa gctagagaaa tcgtagtagg taatcacaat tggggttgat tttttgctga
    15961 taatctcctg tttggtgggc aattgaatct ctgcaccacc tagattaatt ttttgaattt
    16021 tttcgtgctt tgctttatcc ttgtctgcaa cagcagtcca cttgatgtct gtaaatcctg
    16081 ctgtttttag ttgtttttcg agctctggtt ttgttttagc ctctgctaga ttttctggta
    16141 gtttgatgtt atcttttcta acgtcaaaat aggttaatgt aacagggaga ctagtagagt
    16201 aaaaatgatt ggattgatag gtgtgatttc cgatttgaag gcgatcaatt ttatcttttt
    16261 tgtccgcatt gccttcttct actagcagaa caggagttag ttcgacttgc ttaaaacctg
    16321 aatctttcag ctctttttcg acatctgcta aatttttaga agctggtagg ttgaattcaa
    16381 cggcatcttt tttgggaaca tggtaggaga ttgtaattgg gatgtttttt aaagcacgtc
    16441 cttccttcca atcttctccg tcaacagaga cgagttcgac aagattgctt tcacctattt
    16501 ttccaaattc taaatcacta agttccaccc cctgaatatt tttaaaacct gcttcctgaa
    16561 gatgttttac gactgttaca tactgttgtc cataaaggtc atcttttttt ggaagctcag
    16621 cagtcattaa ttgtgaaccg ataaatgttg taagtaggat gccaccagtt attcctgctg
    16681 ttttccgatt aagaaacttt ctagattttt taattgacgt ttcgctaact atctgagttg
    16741 cttttttctt aaacataaat cattctcctt attgtttatt gtgatattat tataacaaaa
    16801 tataaatata ttttgcaata tcttatgaaa atatccttgg agaatcataa aaactatggt
    16861 acaataaacg aaaaataaag gaggaaatat atgccatttg tacgtattga tttgtttgaa
    16921 ggacgcacag aagaacaaaa gattgcctta gcacgtgaag ttaccgaagt tgtatctcgt
    16981 aatactaatg cgcccaaaga agccattcat gtcttcatca atgatatgcc tgaagggacc
    17041 tactacccac aaggtgaaat gaaacgaaaa taagagaaaa cactcttgca agaaaatgca
    17101 ggagtgtttc tttgtatcaa gtttatttct tatcgtcttc atccatgctg agaacactga
    17161 ggaaggcttc ttgtggaact tcgacagagc cgatggcttt catccgtttt ttaccagctt
    17221 tttgcttttc taggagtttg cgtttacggg aaacgtcacc accataacac ttggccaaaa
    17281 cgttcttccg aagagccttg atgtcgctac gggcaacaat tttctgaccg atagctgctt
    17341 ggattggtac ttcaaactgt tgacgaggaa tgattttttt gagtttgtcc acgataattt
    17401 tcccacgttc gtaggcaaat tccttgtgga cgataaagct gagggcatcg accttatcac
    17461 cattgaggag aatatccatt ttcaccaact tggatgaacg gtattcagaa atctcatagt
    17521 cgaagctggc ataaccacga gtggacgact taagcttgtc aaaaaagtca aaaacaatct
    17581 cagccagtgg aatttgataa ataacattga cacgattatc atcgatgtaa tccatagtca
    17641 caaagtcacc gcgtttgcgt tgggccaatt ccatgactgc accgacgtat tcctgtggta
    17701 ccatgatttg tgccttaacg taaggctctt cgatagtggc aatcttggtt gggtctggga
    17761 attcggatgg gttggctaca tcaatcattt caccgtccgt catgttgaca tggtagacaa
    17821 ccgacggtgc tgtcatgatg aggtcaatgt taaactcacg ctcaatccgt tcttggataa
    17881 catccatgtg caagaggccc aagaatccac aacggaaacc aaatccgagt gcctgcgatg
    17941 tttctggctc aaactgtaag ctggcatcgt tgagttggag tttttccaag gcttcacgga
    18001 ggtcattgta cttgttggaa tcgattggat agatacctgc aaagaccatt ggattcatct
    18061 gcttgtagcc cgctagtggc tcgctagcag gattttttgc caaagttacc gtatcaccga
    18121 cacgggtgtc ggcaaccgtc ttgatagagg ctgcaatgta acctacatca ccagtcgcca
    18181 aataatcacg accgattgct ttgggtgtga agatacccac ttccgtcaca tcaaaggttt
    18241 tgccattgct catcatctga atagtatcgc caggttttac cacaccgttg acaatacgaa
    18301 cttggaggat aacgcctcga tacggatcgt aaaccgagtc aaaaatcaag gcttgcaatg
    18361 gagcttcgac atcaccagtc ggtgctggaa ccttctccac gatctgctcc aagatttcct
    18421 cgataccaat acctgccttg gctgatgttg gcactgcttc ggaagcatcc aaaccaatca
    18481 catcttcgat ttcctgacga acacgttctg ggtctgctgc tggtaggtca atcttgttaa
    18541 tgattggcaa gatttccaaa tcattgtcca aggctagata aacgttagcc aaggtctgtg
    18601 cttcaatccc ttgagcagca tccaccacta aaatcgctcc ttcacaggca gccaacgaac
    18661 gcgatacctc ataggtaaaa tccacgtgtc ctggtgtgtc aatcaagtgg aaaatgtatg
    18721 tctctccatc tttagctgta tagttgagtt cgattgcgtt gagtttaatt gtgataccac
    18781 gctcgcgttc caagtccata ctatctagca actgggcttg catttcacgg ctggaaactg
    18841 tttctgtctt ctcaagaata cggtcagcca aggttgattt accatggtcg atgtgggcaa
    18901 taatggagaa attacgaatt ttctcctgtc gttttttcaa atcttctaaa ttcatgtttc
    18961 ttcctctaca aggctattac tcctcattat atcagattta gtgataattt tccataagaa
    19021 aaacacctag aaaactaggt gtctgctctg ctattttccc agccaaagac cgatctctcg
    19081 ttcggctgct tctgcactat ctgagccatg aacgacattt gccacaattc cctcaacggg
    19141 cgctgtcgca aaatctccac gaatagtccc aggtagggcg tttactggat tggtagctcc
    19201 catcatatct cgccaagatt tgataacttc tggcccttct ataatgccag ctatcatcgg
    19261 cccgctcgtc atatattgta ctaattgcgg gaagaaaggt ttatctgtca aatgatcata
    19321 atgttgactg attagctctt ctgtcgctgt aaccattttt aaatctcgga tttgaaatcc
    19381 cctgcgttcg atacgtgaaa gaatttgtcc tgcaagtcca cgcttaagag catctggttt
    19441 gatgatgaag aaagtacgtt ccatgatttt tctcttgaac tgtttattag atgttactta
    19501 agatagcatc ccatgcctga tcgtatccgt gacgagtctc agaagaaaag ataacgaatt
    19561 gatcattttt atcaaaatcc aatttctttt taatcatcga ttcgtgctta ttccactttc
    19621 cttgtgggat tttatcagcc ttcgttgcca caataataac aggaatttca taatatttca
    19681 agaaatcata catttgaaca tcatctgctg atggctcatg acgcatgtct actaaactca
    19741 ccacaacacg tagattttct cgtgtcgtca aatactcttc aatcattttg ccccacttag
    19801 cgcgctccgt tttggatact ttagcatagc cataacctgg aacatctaca aatcgaattt
    19861 tatcatctat attataaaaa ttgagttgtt gagtctttcc tggttttcct gacgtacgtg
    19921 caagattttt tcttcctaga agagtattga taaatgagga ctttccaacg ttagatcgcc
    19981 cggcaagggc aatttctgga atgtcatcgc ccggatactg ggcttttgaa acagcactga
    20041 gcaaaatctc agcattatgt gtgttgattt ccattttata cctttcataa ataagggtag
    20101 aaaacaatca gtttccaccc tttatttttt tatgcagttt ctagtaaagg cttttcctta
    20161 ccgtctactg ctgctttggt tacacgaacg cgtttaacgt tatcttgacc tggcacctca
    20221 aacatgacat ccaacatcgt ttcttcaatg atggagcgaa gtccacgagc accagtttta
    20281 cgttcaattg ctttttctgc aattgctaac aaggcgtcct catcaaagtc aagctccacg
    20341 tcatcataag acaagagtgt ctggtattgt tttacaagag cattcttcgg ttccgtcaaa
    20401 atgcgtacca aatcctctgt tgtcaattgg tccaaagctg cgaatacagg caagcgacca
    20461 atcaactctg gaatgatacc gaatttttga atatcgtctg caatgatatg ttgcatgtat
    20521 gattcttttt catcaatagc acgattatta tgaccaaaac cgatgatttt ttcaccgaga
    20581 cgctgcttga cgatttcttc gataccatca aatgcaccgc caacgataaa caggatattt
    20641 ttggtgtcca cgtgaatcat ttcttggttt ggatgtttgc gtccaccttg tggtggtacg
    20701 ctggccacag ttccctcgat aatttttagg agagcttgct ggacaccttc accagataca
    20761 tcgcgggtaa tggacacatt ttcacctttt ttggcaattt tatcaatttc atccacatag
    20821 ataattcctc tttctgcacg gtcaatgtta aagtctgcag cctgcaaaag tttgaggagg
    20881 atattttcta cgtcttcacc aacataaccc gcctcagtaa gagcggtcgc atcggcaata
    20941 gcaaacggga cattcaaact tttagccaag gtctgagcta ggaaggtttt tcctgaacca
    21001 gtcgggccaa tcataaggat atttgatttt tgcaaatcaa catcgttctc atcacggcta
    21061 tcttggaaat tgatacgttt gtagtggttg taaacggcta ctgctaaagc acgttttgca
    21121 cgatcctgtc cgatgacgta gttgttcaga atattgagta attcttgtgg ctttggcgta
    21181 tcggccaagt ctgtcaaaac ttcttctgcc agttcctcac gaatgatttc ctgtgctagt
    21241 tccacgcact cattacaaat gaagacattg tttcctgcga taattttttt aacttcttct
    21301 tgattttttc cgcaaaatga gcaatagatt agctcatgtg tatgcttaac agccatgtgt
    21361 ttcctctttc atatctctta tctaaataaa aatccataaa tggcgtgaat actattgacc
    21421 agattggtaa aagcattcag taaaaataaa attgctaaac caaaacgttt cttccgaaag
    21481 gcttgaatcg cacaaagaac acaaatccca cagagaaatg ctgtaaataa accgaataaa
    21541 cttctctcca tgcctaacca tctcttcttt cataggtttt tattgtaaaa tcatacgggt
    21601 ttttctcgtc ttttggacgt aaattggcgc tttgtagctg aaatttctcc ataggaaatg
    21661 ctttcggaaa atacacatcg ccattaaatt gaccgtgaat atctgtcact attaatgttt
    21721 caatgtaagg tgcaaaagca gtaaaaattt gtcccccacc aatcacaaat agtgtctgct
    21781 cctgtttgtt ataccaatct agaacatctt ccacactgtg taaaatggta atacgttcac
    21841 tctctagttg ataggtttta tccgttgtca aaacgatggt atgacgattg ggaagagcac
    21901 gtttacccat gccgtcgaaa gttatacgtc ccatcaccat cgtgtgacct attgtcgttt
    21961 ctttgaaatg agctaagtca gctggcaatg accaaggaag tctatcacct tttccaatca
    22021 atccattttc atcttgagcc caaatggcaa caatcttttt agtcattttc tctcactttc
    22081 cgtcctatca ttctatcaaa aaaacatgaa aaatgcactt accaagtagg ccaagcgcaa
    22141 atttttatca tgttgaaact atcaccaact cctcagagat agggaggagt ccgagattcg
    22201 tagaatgtga acctcgctgg tgctggaaag tccccctcat cttaaatcgc taagtcaaaa
    22261 ttaagttgcg gtttaactgg cttcgaaagt ctgagtatag actgttgaag gttggaagtg
    22321 aaacagttcg gggaactgtt tcagcccgag cctaaaaata aagaagcgag gtaaaagaac
    22381 gaagttcttc actgctaatc cagttaaatt gctaaatcga attttaactg cggtttaact
    22441 ggattgtagt ccaccaactc aaaatcttct gcccgaatgt caaagaaatt ggtgccgtca
    22501 ggaacattta agaccaaacg tggctcaact tcagatggtg tgcgacggag taattcttca
    22561 gcctgttcaa actgattatc atagatgtga agattgttga tgaaatagaa aaacttccca
    22621 accttccagc caaaatgttt agcaatcatc atctgaaggg ctacatactg catagcgttg
    22681 atatgatggg ctaccaacat atcatttgac cgttgagtta gagttgcatc caaataaatc
    22741 tccccatcca ctcgccgaac gtcaaacatg gtctgaaagg cacatggcaa aagacccgcg
    22801 gattggtcaa atgcttcata atcccacaaa gaaatcacat tacggcggtt ccatggattt
    22861 tcctccaatt gcttgaggat ttttgaaatg atgtcatgct ttttaacaat tgcaccatag
    22921 cgttcaccga ttgtgcctgt tccttcaact tcccagtcat tccaatactg gacaccgtat
    22981 ttttcctgta aaaccgacaa atcattggtc tggtcttggt aaatccagag aatttccttg
    23041 atggctgatt taatcggaat aggtctaagt gttgtaatag gaaactcccc cttagacaag
    23101 tcatactcag caaaggcgcc tgtaacgtac ttggaattag cgacatttcc atccttgtaa
    23161 cgtgggcgag cattttctga aaaaacgccc tcttccataa tcttacgaat gttttcttta
    23221 aaaataatat ctgctttagt catggtctta gtatagcaaa ttctagcaaa aaactccagt
    23281 tttgtaccgg agttctcatt tgaaaaagaa tgaataaaaa ttaaggtgga acaaaccaga
    23341 tagggcagtt attaggctga acagtataaa atacaaggca accaaaatag cactcaacaa
    23401 tatctgacga acatttttag tataaatagc cgttggtaag ctggctagtc cgaaaaatgg
    23461 aatgatgaaa aatgttagtt taaaattcac atttacaata tcaaaccatt gccctattgg
    23521 caaaaatagc atgacgaaga gaaaaattgc gataccatac aaaactagat ttaatctttt
    23581 tagtaaagtc aggttcataa ccgctccttc taggggccaa atataaagta gccaataccg
    23641 aatatcagag gaaatgagat cattaagaag atactaatca aaccagtcca caatttttta
    23701 taatagatag atagtataag aattagagtc cccagtagag ggagaatcaa catggttgct
    23761 acgtttcccc agctagacgg gaaaaaccag tcacgaatcc agttatcaag gaaaaaagca
    23821 gataacaaaa tcaataaacc aagaatggtc agatagttga ttattttaga catgttcacc
    23881 tgtttcataa ggtgacactc ctttctcttt acactttcat tgtacgctta aatactacat
    23941 ttttcaatat atgtatataa agaaaatgtg acagcggaat tgaaaaaaag cagagtttcc
    24001 cctgctttta acgattattt caagacaagt gatgctgcgc ccaatacacc tgcatcatta
    24061 ccacgtgttg ccaagacaat ttgcgtgctt tccttgattt gtgggaagct attttcagca
    24121 aagactttac gaacaccatc caataagagc tctcctgcag ctgatacacc gccacccaga
    24181 acgatgtagg caggattgag gactgcagca atgtttgcac aagcaacacc taggtattct
    24241 gagaagtgac ggtagacaat caaagccaaa tcatcgcctt cttttgccaa atcaaagaca
    24301 tctttagccg ttacatcttg cccatcatca atcatttgtt tcagttttgc atcgcctgcg
    24361 tattggtccg cataacgacg gctaaggttg acaatacctg tcgctgaagc cacggtttcc
    24421 aagcaacctt tcttaccaca agtacatgca aatggctcat caaagtcaac tgtgatatgg
    24481 cccaattcac caccagcgcc tttgacccca cgaatcaagt ttccagcagc gatgacaccg
    24541 ccaccgacac ctgtaccaag cgtcataaag acaacgtttg gattgttgtt tccagcacca
    24601 acccactgct caccgagagc cgctacgttt gcatcattgt cgatgaaaaa tggtaaaccg
    24661 agcgcagatt caaactgatc tttaaccaat tgcaaggtct tccagttgag gttataggct
    24721 ccgataacag tgccagcttc actatccact acaccgggtg agcccatacc aattcctaag
    24781 aaattgtcct ttgtcaaacc atgggtttca aaacgatatt gaatgctgtc aataatatct
    24841 ggaacaatat ggcttccatc gtctaatata tttgtcttga tagaccattt ttcttgaatt
    24901 tctccctcag tagtcaaaat ggccaattta acagaagttc ccccaaggtc aataccgata
    24961 atttttttag acataatcat cacctcaaat gtattttctt tattataaca caatgcaaag
    25021 gtttgcgtaa agcctttgca aaaatttgta cggataaaaa ttaattgctc tatcgaaaga
    25081 gatagagcaa aattaaaaat tgaatatgta agataaacct tccaatggaa aagagcataa
    25141 ctgtcgtaaa attactacct tctttcgttc cattatagag aaaaagagta gaaataaagg
    25201 taaatcctag agcaaacccc accatctgca agtcgtgata agactgacta taaaaataca
    25261 aggtgcttgt cagcaatatc atggatagga tagttaaaat caagcgcaat gtattcattt
    25321 ttttggtctt tagatagcta aacaaagccg ctcccaaggt caccaagaga tagatgacca
    25381 tatatgacat gacaagaact ttcatctagc cctccagttc gctcagatac tcataccgct
    25441 catatttttc caagagcaga tcgtttttct catccaattc cttctgcaaa tcggataatt
    25501 ttgtaaaatc actaccacag gtcagcattt ccgcctcgat tgccgcaatc cgctcctcca
    25561 agccagcaat atcctcttcg atggttgccc attcctgctt ctcaaagtag gacatccgct
    25621 tcttgttctc tttgattttc tcggttttct cctttggttt ttccaaagaa atggcagagc
    25681 tggaagccag aaaggccttc tcatccagat agtctgtgta gttaccaaag aaggtctcta
    25741 taccgctatc ttcgaaagcc aagattttat tggctacctt gtctaggaaa taacggtcgt
    25801 gactaacggt aatgactgga ccagtaaagc tttgcaagaa atgttccaag actgtcagag
    25861 tcgctatgtc caagtcgttg gtcggctcat ctagtaagag aacattgggc cgttgcaaga
    25921 gaatcttcag caaataaagt cgcttcttct ccccaccaga caatttctca atcagggtac
    25981 catgggtatt acgtgggaag aggaattgct ctaagagttc ggcaattgaa accataccag
    26041 aagtcgtctt agcttcttct gccacttcct gcaagaaatt gatgacccgc ttgctttcgt
    26101 ccaagccctt gatggtctga gaaaaataac cgatacggat ggtttcacca atatctacct
    26161 taccgctgtc tgcctgcaaa tcacctgcga taagattgag taaggtagat tttccttttc
    26221 cattatcacc gacaattcct atgcggtcct tgttttgaat gagcaggtta aagtctgcta
    26281 aaatgggctt atcaggatag gaaaagctga cattttcaaa gttaatgact tttttaccga
    26341 tacgagaggt ttcaaaattg atttccaatt cgctatcgtc tatcttgttg gctaaatcgc
    26401 ctttaaggtc atggaaacga ttgatacggg cctgttgctt ggtagcacga gcttgaggtt
    26461 gtctgcgcat ccaagccaac tcttgtttgt agagctgctc tttcttatgg cgaagggcgg
    26521 catctcgctc gtcctgttct gcctttaagc gaacatagtc ctggtaattt ccctgatatt
    26581 cggttaagct tgcgcgatcc aattcaaaaa tccgtgtcgc cacattgtcc aagaaatagc
    26641 gatcgtgggt aataaagaga acagactttt tagtattttt caagaaagtc gtcaaccact
    26701 caatggtatc aatgtccagg tggttagtcg gttcatccag caagagcaag tcagcattgc
    26761 caaggaggac ttgggccaat tgcactcgtc tacgtagacc acctgataac tcaacaacgg
    26821 ttttgttgag gtctgtcaga ccgagttttg acagaacagt cttgacctga ctttcaatct
    26881 cccaagcatt gagagaatcc atctctgcca tgactttctc cagtcttgct tgacttgctt
    26941 catcgtaatg agataagagc aactcgtatt cacgaatcag ctgggtttcg cgtaggtcag
    27001 atgaaagaac ggtgtctaaa actgtcttac tttcatcaaa atcaggttcc tgggtcagat
    27061 aggcgatttt ataggcattt tttgtacgaa aaggtgatac atctccgtca aaaccgatac
    27121 gacctgacag gacatccaac aaagtcgtct ttcctgtacc attgacccca ataatcccga
    27181 ttctgtcgcc ttgatggatg atgaaagaca gatcagcaaa gaccgtttta tctccgacag
    27241 atttagtcag gtgttcaacg ataaaatcac tcattctcta agattccttt cacaaatgtc
    27301 ataatcgctt gctcttcgtt ttccagctgt ccttccacaa tctggaattc cacctctttg
    27361 agtagttctc ccaatactgg acctggtttg aggtcaaatg ccttcatcag atgtcctcca
    27421 ttgacaacaa tttcatgctt gtcgtggata gtcaaggctt ggtcaatctt atcgattgct
    27481 acaaaatctg tgacaagacc ttgtgcctga cgaagttcct ctaccaagta taagaattct
    27541 ttgccatact tgaaacagat ttgtttagtg acaggtcctg cttggcggag ctgataaatc
    27601 tctactaatt tgaccacgct acgttggaaa tcattgctgg ttttccattt tttcaggaag
    27661 gatttgatgc tatcaatacc taggcagaca aagagcatgg cccaagcttg ttcagatgtc
    27721 gaaaagcgaa actcttctgt cagacttgtt agcatggtct ctaattcatc actttttcca
    27781 gctaaatcag gcaagaaatt gtaagccttg gaatcaatca tagcgcgcaa gccttttcgc
    27841 caaaaatccg ccatcaagag tttatcaaac tcgataaagc tacgttccac cgagattttt
    27901 tctaaaagcg gtgcagtttt aaccatggct tcaaaggtag tcggctcaat ttcaaagtcc
    27961 aaggtcgctg caaagcgaaa tccacgcatg atacggagag catcttcgtt gaaacgctca
    28021 gccgggatgc ctacagcccg tagggtgcgg ttttccaagt cagtcatccc ttcaaaaagg
    28081 tcaatgattt gagcctcttc atcaagggca aaggcattaa ctgtgaagtc gcgacgtttg
    28141 agatcctctt ctaaggaacg cacaaaagaa acctgactcg gcctacggaa gtcgacatac
    28201 tcctcttctg tccgaaaggt ggtaatttca tactcctttt tcccctctaa taccaacacc
    28261 gtaccgtgtt caattccgac atcaatagtc cgtgaaaaga tttgtttcgt ttcctgaggg
    28321 tagctggatg tcgcaatatc gacatcatga ataggcctac cgagaatggc atcacgaacg
    28381 gaaccgccaa cgaaataagc ctcgtaacca gctgctttaa ttttctctaa aatcggcaaa
    28441 gcctcctgaa actcagaagg cagattgttt aatttcataa taagtgttcc aaaccataaa
    28501 cgagttgtga gcgtttgact acctctttaa tgccgagatt gacaccgccc ataaaggaaa
    28561 tacgatcgta agagtcatga cgaatggtca agccttctcc ctgtgcccca aaaatcactt
    28621 cctgatgggc aacaaggcct ggcaagcgta ctgagtggat acggaagcca tcaaatgctg
    28681 caccgcgagc gccagcaatc agttcttctt catccgctgc accttgagtc tgtgactgac
    28741 ggacttgtga aatgagttcg gcagttttga cagccgtacc actcggtgca tccttcttct
    28801 tatcgtggtg taattcaatg atttcaagat ttgggaaata cttggctgcc tgtgctgcaa
    28861 attgcatgag caagatagcg ccaatagcaa agttaggagc aatcaagccg cccaaaccct
    28921 tctcagcaga taaggcggtc aattcctcga tttcttcagg ggtaaatcct gtcgttccaa
    28981 ccacaggtgc gaaaccattt tccaaggcaa aacgggtgtt ttcataggca aattttggcg
    29041 ttgtaaaatc cacccagaca tcagcttcta ggctggcaac ttcttctttg gtcttgaaaa
    29101 caggaacacc atctacttct gtttctgtcg caaagggatc caccaaggca gccaaactaa
    29161 gtgctgcgtc acctttgacc atctcaacag ccgttgagcc catttttcct ttgaatcctg
    29221 cgataattac cttaattgtc atgcaaactc ctattcaata atgggagaaa taccaaaggc
    29281 cactgcccct tctcccaagt gcgtaccgat gaccgaacca aatgacacga ttggtaaatc
    29341 gctaccgaca cctgcttctt ccaactgctg tttaaagttt tcagccttct gaggtgcgtt
    29401 ggcgtgtatg atagcaatct gatactgtcc ttcggtcgtt tgttcttgga ggatttccaa
    29461 gaggcgttta atggcctttt tctccgtccg aactttttca tacacttcaa tcttaccctc
    29521 gcctgtaaaa tacagaatcg gtttgatgct caaaagattg ccaagcaagg ctgcaccatt
    29581 ggacaagcga ccacctttta ccaaatgatt gaggtcatca accatgatga aggctgtagt
    29641 cttcccaatt tcttggtcca atttcccagt aatctcttca aaactaagtc ctgcatcagc
    29701 ccatttgaga acattttcga ccatcatgcc taaaggagcc gatgtaattt tggtatctgg
    29761 aaaggcaacg gttaatcctt caaattcatc agccaaatat tgaatatttt gataaaaacc
    29821 tgaaatacct gatgacaaga ataagccaag tgcgtgtgta tagcctttct ctgttaattt
    29881 agccagaatt tcttctagtt ccatcaaact cggttgactt gttttaggta attctttcga
    29941 tgaagccatt ttttgataaa actcttcaac cgtcaaattt tgcccttcta tataattttc
    30001 cccatcgatg ctgactggaa tacttaggac aaacaggtca tcccgctcaa tactttgaac
    30061 attcaaaata gccgatgagt cagttataac tgctaacttc atattaaaac tccaaattca
    30121 ctcctggtgc atcaaaggcg atttcagtaa cctcataggt cattcgtttt aaaatgtcca
    30181 aaagaggctc taccaaaaca cgtttttcct catcagtaaa ttcagttggc tcttctacca
    30241 atcgatttgg taagtgaacg gcctgactca tggcacctga aatcacaaaa tgatctagta
    30301 caatcataaa gcgcaaaatg gtaatcatcg atgttcgatt ttccgcacga ttttgctcaa
    30361 tcaattgaaa gtcaactttc aattttgttt ccggaattcc gttctctttt tcccacgcgt
    30421 gattgcgggc atcaaaatga tactgattga caaattcttt atcgcgaatg atttccattt
    30481 caagactcct ctaataacaa taatactcac attataccat aatcctcgtg aaatagctaa
    30541 aaaaccttgc cagaagcaag gtttcaatca aaattattta gctttacgaa gtgctccgta
    30601 aaggacacct gatacaaccg caccgatcaa aacataggcg atgtaaagca atggattaga
    30661 tgtcaaagcg ataacgaaga taccaccgtg aggagccatc aattggatgc ctgacaaacc
    30721 aaccaaggca cctgcaaggg cagaaccagc gataaagctt gggattgcac gagctgggtc
    30781 agcagcacca aatggaatcg caccttcagt gatgaatgaa agacccataa cgatgtttgt
    30841 caaacctgag ttacgctctt cctgtgtgaa tttgtctttg aaaagaaggg ttgcaacgaa
    30901 tactgccaac ggtggaacca taccacctgc cattaccgct gccatggcaa cagaaccacc
    30961 atctgcaaca gttgctgcaa gtgtacctgt accgaagaca taagctgcct tgttgactgg
    31021 accacccata tcgactgcca tcattccacc gaggacaagt ccaaggagaa tagcagagct
    31081 accttcaaga cctgcaagga agttattcat gcctgtgttg atagctgcca ttgggatgtt
    31141 aacgaagaac atcaagaaac cagtgattgc tgttcccaaa agtggcaaga gcaagatagc
    31201 gttcaaacct tgaagtgatt ttggaaggtt gcgcaaagcg ttgcgaagaa cgagcactac
    31261 accaccagca aggaaaccac caacaagggc acccaagaaa ccagatgata cacctgcaag
    31321 agcaagcgtt tcttcaccac cagctgcgta aggaatttta ccgaacgcaa gaccgctact
    31381 agcaattgca ccagctacga aacctgcaac caaacctggt ttttcagcga ttgagtaagc
    31441 aatgtaacct gctagaagtg gcaacatgaa gccgaaggct gctccaccga tcttcatgaa
    31501 catagctgcg atttcatggt atgaaccaag actaccaagg ctgtcttgtg gtacacctaa
    31561 agctccatcg aataggaagg caagggcaat caagataccg ccaccgataa cgaatggcaa
    31621 catttgagaa acaccactca tcaagtgttt atagaacgct ttgccaagac tgagtttttc
    31681 tgaagattcc acagtagctg ctccttctga cgctgtgtag gctgctgctt taccgtttaa
    31741 gatgatgtta atcaattctt ctggttgttt gataccagct gcaactggac gagataccaa
    31801 tggtttgcca ttgaaacgag gcatgtcaac agccttatct gctgcgatga taacacccgc
    31861 cgcattcttg atgtcttcag cagtcagttt gttaccaacc ccagacgcac cgtttgtttc
    31921 aaccttgatg ccaacaccca tctcagcagc ctgcttctta agagcttctt ctgccatata
    31981 agtgtgagca ataccagttg tacatgctgt aactgcaacg ataaatggac ggtcgccact
    32041 aggagctgca accacttctt ctactgcctt gtcagctgct tcagctgtat cgaagactgc
    32101 aatcacttct tcaggatttg ttgccgcacg aagtctatca gcaaatccag ctttcatcaa
    32161 gtatttagac aattccgcaa gggctgccaa gtgcgtgtcg ttggcacctt ctggagctgc
    32221 aatcatgaag aacaagtcag ttggttgacc atcaagactt gcataatcaa caccattgtt
    32281 tgattttgca aagagaacag tcgcttcttt tacagccgca ttcttagcgt gtggcattgc
    32341 gataccgtca ccaagtcctg ttgtcgtttg tgcttcacgg ttcatgatac ctgttttgaa
    32401 aacatcaaaa tccgttacat aacctttatc aaccaaacta gcaatcattt catcaataac
    32461 tgcttccttg cttgtcgctt gcaaatcaag caacatgaca tcttttctca aaacgtcttg
    32521 aattttcata gtgtttctac ctcaactttt tcataagttt ccttaataaa ttcgatactt
    32581 gccaaatcat ctgaaaaggc tgttgccgta ccgcaagcaa ctccccattt tagggcttca
    32641 attgggtctt gtgagcgaac gtattcgcca gtgaatcctg ccaccatgga atctccagct
    32701 ccgacagagt ttttcactgt acccttgatt ggtttggcaa agtaagtagc tgctggagtg
    32761 accaacaaag ccccatcccc tgccatggaa atgataacat tctgagcacc catttctaag
    32821 attttcttag cgtaggtctc gatatctgca atgtcattca actcgacgtt gaagattgct
    32881 tcaagttcat ggttgtttgg cttgaccaag agtggctggt gtgctagtga gtctagaagt
    32941 gtttgacctt caaagtcaca gaccacttgc gctcctgctt ttttagccac aggaatcagc
    33001 ctattataga cttcatttcc aagactagct ggagcagacc cagcaaagac aacggtgtct
    33061 tctttggtca atcctgctaa gaccatttct aattcggcca actgtgtatc tgtcacaatc
    33121 ggacccaaac cgttgatttc tgtttcttgg tcagctttga ttttcacgtt aatacgtgta
    33181 tcttgatcaa cctgaacaaa atcagtatca attccttcgg caatcaatcc atctttgatg
    33241 aactgacctg tgaatccccc aaggaaaccc gttgctgtat taggatagcc caatcgcttc
    33301 aaaacacggc taacgttaat tcccttaccg ccggcatact tatcgtcgct ctccatacga
    33361 ttgacactgc ccgtctcgac atgttctaaa cgaacgatat agtcaatcgc tggattgagc
    33421 gtaactgtat aaatcatacc tcgattaccc tcgttttctc ttttatgatg tccaacaaac
    33481 tgctttctga agattggcag ataatgttgg atttttcgat tgccgcaact ttaacaaagg
    33541 agaattgacc aattttagaa gcatctgcca aaacatagct ttctttagca ttgtctatga
    33601 tagtgcgttt gatggtcgct tcttcaatat ctggcgttgt aaaatagttt ttatctatac
    33661 cattcatccc cagaaatgcc ttatcaaaat tgagctgtcg aatttgttcc aatgccactg
    33721 ctccaacgga agcatctgtt gattgcttaa caaaaccacc tataatgatt gtttttatct
    33781 tgcgttcaac cagcctcact gcgtggtgaa tagaattggt aacgactgtc agattttcct
    33841 gctgaagata atcaatcagt acaccggttg ttgtacctgc atctaaaaaa attacgtctc
    33901 cgttaacaat ttgttgagca gctctttccg caatctctcg tttttcttga acgtttttga
    33961 cagatttttc tagaatagac tcttcgagtt gcaaattcct tggggcttca gcaccgccgt
    34021 gtacacgacg aagcttacct tcgttttcca actcgtccaa atccctacgt acagtagact
    34081 cagatgtatc aagaacttcg attaattcct ctagccgaac aaactgttga gagtgtattc
    34141 gctccaatat aatctgtttt cgttctgatt tgagtatgtt caacacctcc tgcaatcgtt
    34201 tacaaaaatc attatacacg attttctgcc attgtcaatc attttctatc attttctttc
    34261 aaaaaaacaa aaaaactagc agaaatctac gcttccacta gtttctcaat catttttttc
    34321 atcatatcca tttcttctgg acgaccaata gataggcgaa tatgcccctc taatttacct
    34381 gttggataat atttgaaatt ccaatcctgt gccaaggctt gctgataaaa ttcttctgcc
    34441 catggtgctt taaacgttac aaaactagcc tgactaggta gaacttgaca gttatctaac
    34501 ccttgcaaga ataaaatcat ttcttctcgc aaattctgaa ttactcccac tgtttctaaa
    34561 accttgtctg tataattcaa tgcaacggct gccatttttg cagtcaagtt tgacaagctg
    34621 aatgggggaa tgaccttgtc caattcataa attaactctt cgcttcctac cgcaaagcca
    34681 actcgtaaac ctgcaagtcc aaaagccttt gataaggtcc gtaaaactaa aacattgtca
    34741 aattgttgca gacgactgat gaagctttcg acatttgcaa attcaacata agcctcatct
    34801 acaacgagta gccctttaaa agaaatagcc aatttctcca aatcttctag tgaataagct
    34861 actgaagaag gattgttagg attggaaagc atgattatct ttgccttcac ctcctctgca
    34921 cgcgccaaca atttctctac tggcaggacg agcgtatcgt tttttgcttc caaatcaatg
    34981 gtttcaaaac ggctaccatg catgtgatta taggtttcat acatgaagaa gtctggattg
    35041 acagtcaaaa aagtatcatc cttttccata aaggtggaaa caatcatgtg aataaggtga
    35101 tcagagccca cacctactgt tacttgtttg ggattcactc caagataggc cgcatatttc
    35161 tcgatcagtt cggagtaagt gttatcacca taaaaactta attgattgac atccaaattt
    35221 tccaaggtct cttgtggtaa aaaggaccaa tcaataatgc gattttcatt atttcccaag
    35281 tcaaacctag ccttttgttt gattttataa ggttggtaat gttcaaatac cttgcgtttc
    35341 atcatttctc cttctagcaa gaaaaacaca gtctaatcaa ctgtgtcttc tgccttgatt
    35401 ttttcaaaaa tagttaactc ttcagctgta aagttgtagt tttccaacaa gtctggacga
    35461 cgttgatagg ttttgcgtag actttcttcc aaacgccatt tacgaatatt ttcatggtga
    35521 ccgctcatta atacatctgg aactaccatt ccacgatact cgtatggtcg ggtgtattga
    35581 ggatattcca gtagaccaga tgaaaaacta tcatccgtat gactgacttc tttgccaata
    35641 acctctggaa tcagacgaac ggtagcatca atcatggtca tagctgccaa ctctccacct
    35701 gtcagaacat agtctccgag agaaatctca tctgttacca aggtcttaat ccgctcatca
    35761 tatccttcat agtgaccgca aatgaaaatc agctgctctt cctgtgccaa ttcttcggca
    35821 taggcctgat tgaaagtacg cccagcggga tccaatagga taacacgtgg cttggcttgt
    35881 tcaatgctat ccatggtatc aaaaattggc tgggcacgga gcaacatgcc ttgtcctcca
    35941 ccatagggtt catcgtccac atggcgagcc ttttcagctt tctctcggaa attgtggtag
    36001 ttgatttcta gcaagccctt gtcacgcgcc ttacccacaa tagagtgttc tagaggtgca
    36061 aacatctcag ggaagagggt caaaatatca attctcatcg tctaggccct cgagcaattc
    36121 cacatcgaca cggttatttg gaatatcaat gttcaagaca acaggtggaa tatatggcaa
    36181 gagcaggtct ttcttacctt ttcgtttcac cacccagaca tcattggcac ctggctgcaa
    36241 gatttccttg atttgtccga tcagaatatc attctcatag acatccaagc cgataatttc
    36301 atggtaataa aactcaccat cttcaaggtc agtcaagttt tcctcggcaa ttttcaaact
    36361 aaagcccttg tacttttcaa tatcgttgat atggtacatg cccttaaact tgataatatc
    36421 aaagttctta gccttacggt gattagcaat ttccacttcc atgacaaact gatccttgtc
    36481 atcaaaaagt gcaagctttg cccctttttt aaaacgttct tctgcaaaat cggtcacaga
    36541 caaaacacgc atttcccctt gtaggccttg cgtgttaaca atctttccta cgttaaaata
    36601 attcataatt tctccatttg attttctatc cttttatttt aacatgagca agatattttc
    36661 acaagagaga aaaagccgga tgtctccgac tttcatagtt aatttatttc acggacaaat
    36721 gagaatgagc tattatgatc aagcgcaaat taagagctat tccgcagaca aaaatccctg
    36781 aaataagacc gatccaatag gcttctggac ccatttggaa aaatctttca agcaagaatg
    36841 cgactgggaa ggtcatggac caataagcaa ccaaaccaag tataaatgga acagtcgtat
    36901 ccttgtaacc acgtaaaatc ccctgaatag gtgctgtaaa ggcatctgct aactggaaca
    36961 tgagggcaaa gctcaggaaa tgcgaggtca aacggataaa ttctgcgtca ttaccataaa
    37021 ggtaggcaac tttggagcgg aaaaaatata ggaaagtgag tgttaggact gcaaaaccaa
    37081 gtgcaaccaa gcgtcctaga cggctgtagg ctttcacatc ctgtggtcgt ttagccccta
    37141 cttcataagc aatgacaata gctagggctg aagaaactga ggcaggaaag gcatataata
    37201 atgtcgcaaa gttcatagct gactggtggg ctgctataat ctgtgctgaa aattttgcca
    37261 tgtagagccc tactactgca aaaattgcta cttctgcaaa gacttgtaaa ccgatgggaa
    37321 gaccgatttt caaaccttcc ttcaataaag aaaggtcaat cggactccac tgccaaatct
    37381 gataagatgc aatagtctta tttttgcaca tgacaatgac gattactagc aataccgccc
    37441 agtaagctaa agcagtaccc aatccagctc cagctccgcc cagggccggt aggcccattt
    37501 tcccataaat taagagatag ttaaatagac tgttaaatgg gacaaggagc aacatgaggt
    37561 acatggatag ctgagtcaaa ccgagagcgt cgaaaaaaga gcgacagaca ctaaagagca
    37621 agagaggcag gatgccaatg gaaataaaat agaggtactg ccgaccaact tgaaaaatag
    37681 attcatcaag tccaaactga gccaaggcgg gaacagcgat aaataggacc gagcacaaga
    37741 gtaaaatggt taatccgatg gctagataga caaactgatg aaattcctca cgaatcttat
    37801 ctctttcacc ttttcctaaa tattgagcga tgattgggac caacgcagat acaatccctg
    37861 ttagaaatga aaaaagcgga ttccaaagac ttgttgccat ggaaacacct gccaaatcca
    37921 tggtactata ctgaccagtc atcatagtat caatgaagga ggctgaataa ttagcaaatt
    37981 gataaattaa aataggcaaa aatattttga taaagagcca gagcttctct ttgttatttc
    38041 ttgtttggta catacttatt ctctttcaat tttatataaa tattgtaaca agaaaaggac
    38101 ctgcattgca agtcctcttt gactagatag agtcaccatt ccagatagag tttttcacga
    38161 taacatagtc cacatgcttc aagctggtaa tgtcacgtcc tcctgcataa gaaatagatg
    38221 actgcaaatc ttgctccatt tcaaccagag tatctttcaa atgtcctttg gcagggagta
    38281 agattttctt accttctacg tttttatagg cacctttttg gtattcagat gcagaaccgt
    38341 agtactcttt gaatttctca ccgtcaactt caattgtttt acctgggctt tcaatgtgac
    38401 cggcaaagag cgaaccaatc atgaccatgc ttgcaccgaa gcggatggat ttggcaatat
    38461 ctccgtgggt acggatacct ccgtccgcaa taattggttt acgggcagct tttgcacacc
    38521 aacgaagggc tgccaactgc caaccacctg taccaaaacc agtcttaacc tttgtaatac
    38581 atactttacc tggaccaata ccaacttttg tcgcatctgc accagcattt tctagttcac
    38641 gaactgcttc tggagtaccg acattacctg caatgacgaa cgtctcaggc agttctttct
    38701 tgatatgttg gatcatcttg ataacacttt ctgcatggcc atgagcaata tcaatggtga
    38761 taaactcagg ggcatcagct ttcaagctag tcacaaactc atattcatat tcttttacgc
    38821 caacagagat agaagcaatc aaaccttgct cgtgcatgcg tttgataaat ggaatacgac
    38881 cagcttcatc gaaacgatgc atgatgtaga aataaccatc tttggcaagc atttctgcca
    38941 catcttcatc aataatagtc tgcatattag ctggaacaac tggtaattta aagctatatt
    39001 tcccaagagt cactgttgta tctgcctctg aacggctatt gatgatacat ttatttggaa
    39061 tgagctgaat atcttcgtag tcaaaaactg gagtttcgtt gaacatgctt ctacctcttt
    39121 ttcttaacgt tggaacagaa aaacggtttg tttttcgtat tttttcctta ttacaagata
    39181 tagtatagct tattgtttgt gttttgtcaa taaaaaagaa acctatctag acaatattcg
    39241 gatatatcac ctttaaggct tgtttataaa atataacatt cgtataattc ttttctgagt
    39301 tactattctt ataaaatttt aaaaaatgtt cagaatttac tcaaacaatt ttacataaaa
    39361 actaaaaaaa tcttcaaaaa cagaagattt tttagtcaaa ttcatataat tgtgggaatt
    39421 tttcgcattc agggttctta ggatggcaaa cctcacgacc aaaataaatc atagcttgat
    39481 gagcagggag ccagagttca ggaggtaaga cttccatgac tcgtttttct gtttccaggg
    39541 gtgtagccga ttttttgaca atatcgtggt gtttacaaat ccgtcccacg tgggtatcaa
    39601 ctgcgaaggc gggaattccg aaaccgacac ttaaaacaac attggccgtt tttcttccca
    39661 ctcctgccaa ggcttctaat tcctcacgag tctgtgggac tatgccatca tgtcggtcca
    39721 acaactgttg ggcacattct tttaaaaact ttgctttatt acgatataag cccaagcgag
    39781 atatataggg ctcgatgtcc ttcacttgtg ctgctgccat ggcttgaggc gttgggaaag
    39841 cctcgaacaa acctggggtt gccttattaa cggcagcatc cgttgtctga gctgacaaaa
    39901 gtactgcgca gaccaattca aaatggttgc ggaaatccag actaggctta gcatcaggat
    39961 agagggcgat gatttcttca ataacttttc ttgcacgttt ttttgataat accatgaatt
    40021 cattatagca tgaaaacttg gatttatcta aaatctggat tgtcataaac ctcattcctc
    40081 gtggtataat aagaagaaaa gggggaatat gtatggactt acaagaactc tgtagaaagg
    40141 tcattcaagc aaggccacgc aacagccata agggaagcta tggtcgagtc ttgctgattg
    40201 gtggactgta tccctatggt ggagcgatta tcatggccgc actagcaacc gtcaacagtg
    40261 gagcaggctt ggtgactgtt gctactgaaa aagataacat accagcctta catagtcatc
    40321 tgcctgaagc catggcattt tcatttgacg atcaagcctt gctgataagc agcttagaaa
    40381 atgctgactt ggtattgatt ggtccaggtt ttggggaaaa tagcagagct gaacagttat
    40441 tggactttgt ttttgaacat ttatctgacc agcagatttt agttcttgac ggctcagccc
    40501 ttaccttagt cgcaaagcaa aacagaaaga acttcccgtg taaacaagtc atcctgacac
    40561 cccaccagaa agaatgggaa cggttatcat ccattccgat tgctgaccaa acaagccaaa
    40621 gaaatcttga agccttgcaa tcttttcaag aaaatacaat tcttgtagct aaaagttctg
    40681 ctacacaaat cctaaaaacc cagccaaacc aagtcacccc aattggagct ggtggtcctt
    40741 accaagcaac aggaggtatg ggagatactc ttgcaggtat gattgcagga tttgctgtac
    40801 aatttcccca tgttcaacta tatgaaagaa tccttgtagc aacctacctc cactcctata
    40861 ttgccgacca actggctcaa gattattatc ttgtaaagcc aactgatgtc tcaagaaaca
    40921 tccaaaagac aatgcttcaa tttcaaacac aaacaaccgc ctaatcaatg attaagcggt
    40981 cttttatata acaaactcgt gattaaagcc tgacaaatgg tagaaaatga tactcaatg
//
LOCUS       DASFSW010000003        40980 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.003, whole genome shotgun sequence.
ACCESSION   DASFSW010000003 DASFSW010000000
VERSION     DASFSW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40980
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.003"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            217..1041
                     /locus_tag="U1099_000124"
     CDS             217..1041
                     /locus_tag="U1099_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866477.1"
                     /translation="MITVQHIHKQFGDKVALDNISFDIQEGQIFGFLGPSGSGKTTMI
                     KIITGQLPADSGKSYLLGKESTLLSPEDFEAMGLVSDSSGFFEKMTLENNLLFYAKFF
                     DVSPKEVEVILQKVGLLDSKKTIAEKLSTGMKQRMLLARALINSPKVLFLDEPTSGLD
                     PTTSKRIHELLLELKGKGTTIFLTTHDMAEATFLCDQLVLLNQGNLVEKGSPQDLIHK
                     YNVTKKVRVTFEDGSDTELLFSELTPEKLAQATTIHSCEPTLEDIFIQQTGATLNV"
     gene            1034..1768
                     /locus_tag="U1099_000125"
                     /pseudo
     CDS             1034..1768
                     /locus_tag="U1099_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2103..2831
                     /locus_tag="U1099_000126"
     CDS             2103..2831
                     /locus_tag="U1099_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEM4866478.1"
                     /translation="MNMFWSIFKSVIHRRDVALFYAFAGLPILVPILSKFLVGVKAEY
                     TDNFLDFLGAALATQDGIVLPILLLSLIISAVFRDEIDSGILFLYKDLNRTRLFNAKI
                     ISLVTMYASYVLLTMLTSAIAYFGFLNASGKVVSDDWNNVQSTFLSIFATISINVIGI
                     LLVAMVSIKAKSLQAVLAGVFWSLFTTTAPFLIGVRYVVPNGYAQMSFNQPLLAWSLV
                     AAITTLYIVATYLKGRSNFEKLEF"
     gene            2841..3419
                     /locus_tag="U1099_000127"
     CDS             2841..3419
                     /locus_tag="U1099_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4866479.1"
                     /translation="MVIRYIRPVALSIALIILASISYLLATSLVLFSPSQFLQVAYLF
                     SIMVGMPVGFILFPSMLTKRYQLCQELSEVKFSWKSFVLLAIAIFLINFWCIQSDEYV
                     NQFIIATCEEFLFRYLIYRILKSEYPTWLAMLVTSLLFGVLLHMNYPLLDNLIIRTPL
                     GLLFSVLATRFGLQYAIGGHWIYNLLVSRFPF"
     gene            3434..3862
                     /locus_tag="U1099_000128"
     CDS             3434..3862
                     /locus_tag="U1099_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866480.1"
                     /translation="MIFILLLSLLYTCLTVVSSQLLLSLSVPILLLLTYLLPPIVNSI
                     CLGLLKDNSWKAWSAGIFPTMSMIFYAAFAYITSSNGQWAQFSQLNTVSDDVMSVEVA
                     SNLLAPSQILFVAVVYYGVAIATYFIQQTSRQKEKGVTYA"
     gene            3855..4727
                     /locus_tag="U1099_000129"
     CDS             3855..4727
                     /locus_tag="U1099_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866481.1"
                     /translation="MLKINHLSKQFAGNEFYSLKDVSLEINKGEIVGLIGKNGAGKST
                     LMKLIAKSLKPSSGTITYKGTDIFSQDNLLADFGIMIDPVFYPEMTVMDNLKFYLDLH
                     GKKDLYPNIEKTLKLVELWEARNRKPKGFSFGMKQRTALAIALVAEPDFLILDEPFVG
                     LDPIGVQKLIDILKQWSSERQISMLISSHQLGELEALCSRYVYIESGELAEAFEGKSH
                     PSVLVQLNTTKNLDSVEALLNDHVVLEGEVLEISTATPTSELNQIFGVLARQELIVKL
                     EVKENHLKEIFTKG"
     gene            4733..5503
                     /locus_tag="U1099_000130"
     CDS             4733..5503
                     /locus_tag="U1099_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866482.1"
                     /translation="MKQIFPAIFKTIWKRKETQIYLLFTLFPFIYLVTSFIEGSNFMQ
                     IHASEGSVSLVAFTNMMMSSVDSFILPSLTLYFLAISVFRKEVDEHTMFLYRDLGRKQ
                     IFWSKYLGLLATIVIFYGLFFATSAFVHYVRVIHLPFGSPSVFDTSLAESLSNVFCIV
                     AYLLKDILSVSLATALCLYLKNITTMIIGFLVTITMMILAVVGGPVAMIFPTSYMPLA
                     SEGLTGVGLAYLGAIGVTLVYALVFTKIAAKKFKNLEF"
     gene            5506..7218
                     /locus_tag="U1099_000131"
     CDS             5506..7218
                     /locus_tag="U1099_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866483.1"
                     /translation="MNRRLVGVLLKSLNKTMFLFGIALSALGVGMGLFLPQFIGQLLD
                     QTYLSNLLTRPELLAGFILFFVSVYTVQALSNYFIGRSGGNALKQLQQYIYESLLTTY
                     VKDLDQYQSGDLASRLTNDMSVVLNFITVILPNFLMNGLMVIGSIYFLWTISPWLTGL
                     SLLLLPMLSMVMIPMNQRLEGYYSAYQEGLGQVSSRISHKFTTIRLMKAFQGEKHEQR
                     EMGKSFQKLSQTFERMIRLSAVQHTLVSSLMTGFIILMLLVAGIEVTKGVMTMATLTT
                     FVLYMMQLIDPVTDIASSLNELTEFQAVSKRLVELLELTKEEQADTELVDEASIQLEN
                     VRFSYHTDPVLNGVSVRIPEGAHVAIVGPSGAGKSTIFALLMKYYQDYQGEIRIGQQC
                     LSDISTKEMRRLISFIPQDNTLFHGTIRENLLYGKNESVSEERIAHILKELGLSHLVA
                     ELEDGLDTRISENGTGLSEGQKQRFSIARALLLEHPIYLLDEATASLDTVTERVISKA
                     IDRLTAGKTRLTIAHRLHTVREADAILVLDKNGQVADYGPHQQLVERNHLYQDFLRGL
                     PQAS"
     gene            7320..7493
                     /locus_tag="U1099_000132"
     CDS             7320..7493
                     /locus_tag="U1099_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866484.1"
                     /translation="MKQTTYTLSLGGNDSGDSKVSKMEKKFEKYMEGIAERLRRSGKR
                     SIDKWFIRVRIEE"
     gene            7788..8789
                     /gene="ruvB"
                     /locus_tag="U1099_000133"
     CDS             7788..8789
                     /gene="ruvB"
                     /locus_tag="U1099_000133"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEM4866485.1"
                     /translation="MTNRILDMEQMQDEEYVERTLRPQKLNEYIGQDKVKDQLKIFIE
                     AAKLRDEALDHTLLFGPPGLGKTTMAFVIANELGVNIKQTSGPVIEKAGDLVALLNDL
                     EPGDVLFIDEIHRMPMAVEEILYSAMEDFYIDIMIGAGEASRSVHLELPPFTLIGATT
                     RAGMLSNPLRARFGITGHMEYYELADLTEIVERTADIFDMEITHEAAIELARRSRGTP
                     RIANRLLKRVRDFAQIMGDGLIDDNITDKALTMLDVDREGLDYVDQKILRTMIEMYGG
                     GPVGLNTLSVNIAEERETVEDMYEPYLIQQGFLMRTRTGRVATAKAYEHLGYPYTEK"
     gene            8789..9535
                     /locus_tag="U1099_000134"
     CDS             8789..9535
                     /locus_tag="U1099_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866486.1"
                     /translation="MAEQMRRVARLFGDWPETIIWTCLEGTMGDIYVDDSQSPQSALA
                     LYGRQSFFGFLAGQPHRDLLKICEGKNIILVPQNQAWSDLIEEVYGDGVRFFTRYATK
                     KDTEFDLGHLQKLVDDLPESFDMKLIDRNLYETCLVEEWSRDLVGNYIDVEQFLDLGL
                     GCVILHKGQVVSGASSYASYSAGIEIEVDTREDYRGLGLAKACAAQLILACLDRGLYP
                     SWDAHTLTSLKLAEKLGYELDKAYQAYEWR"
     gene            9537..10175
                     /locus_tag="U1099_000135"
     CDS             9537..10175
                     /locus_tag="U1099_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEM4866487.1"
                     /translation="MTTPTFIWDLDGTLLDSYEAILAGIQETYEQFDIPFDREEVRNF
                     ILRYSVKDLLVRDADKYGLDSDELNHVRATSLKEKNTQIPLMTGAVEILNWTAEQGIQ
                     NFVYTHKSDNAFQVLEDLGIRHHFTEILTSDSGFARKPSPEALLFLIEKYGLDKESTY
                     YIGDRLLDVETAINAGIRSINLQINGVEQNWKIVSLLDIKQILTDKSSHNNL"
     gene            10422..11339
                     /locus_tag="U1099_000136"
     CDS             10422..11339
                     /locus_tag="U1099_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM4866488.1"
                     /translation="MNDKEFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGAS
                     KPTFSKIQYIATRLGMGLYELMPDYVSLPERYSKLKFDVLRTPTYGNEDLAEKRDAMM
                     TEIYDDYYDELPEEEKIVVDTLCSLFDVLDTDSQEYGKEILDDYLHQSYHRSKLSIND
                     LMILRLFIEHCQLEDLSVGTSNYALFTDLIEKLPQSIYDVHSESLFIVRDLFLAIVRI
                     LFSKELYNHIPVYIEKIENIMELSQDFQKKPILNLVKWKYELKVQHNHEIAERYYNEA
                     ITFASLLNQFHLKEKLQMEWEKDTQSLKR"
     gene            11604..12845
                     /locus_tag="U1099_000137"
     CDS             11604..12845
                     /locus_tag="U1099_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEM4866489.1"
                     /translation="MLENWLNTKQGQVFHYKMEKIEYALELLGNPQFAVPVIHVAGTN
                     GKGSTIAFMRQLFQTHGLRVGSFVSPHMVSVHDRICIDSQPISDHDFQHYLQKVYDLE
                     QEVATRYEPFRYFEVMVLIMFLYFEAQQPDVALVEVGIGGLLDTTNVVAPALSVITSI
                     GMDHQDLLGSTLGEIAEQKAGIIKENVPVVLGPLCPETTAICRHIAQDKQASVYQFGQ
                     EFTYKAGQFSNADLELSELVLGLAGHHQEENAAVALQTFLLYMTNIQKDIQPQLIQQA
                     LAQTSWPGRLELVAQEPKIYLDGAHNVPAIERLIEFIQVQEEPVTILFSALRRKDFQE
                     MLELLDEKLPHTALVLTSFAYDGALSEENRQGRDYVENYRQFIEAWQSSKQGILIVTG
                     SLYFISEVRRIFKNEYLFANK"
     gene            13014..14537
                     /locus_tag="U1099_000138"
     CDS             13014..14537
                     /locus_tag="U1099_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HEM4866490.1"
                     /translation="MTELDVVSDVVTHPEETYRRSRRASRRQQVQLNGEFHERVLKTK
                     YWPALVWSLVLSVFSVANPLLMPFATNMQTQNLYAGMAMANGQIPYGDFFGTSGLLFY
                     LLAFLGHLGGTFIIFGILQFIALLIAGVYFYKVVAYFSQSEHLATSSSHWFYVFIFAL
                     GFGGMYAEMFALPFLLTSVWFLVRYFENAVRDEAFILYGIDAALVFLIYPKSLILWLV
                     AGLVLFIFNIQHRQVTRGIYQLLATIFGFLLILYAVGYYAFEAQILGTAIQQTFLYNL
                     QLDFHHSYLYLALAIVSVFLLLSGFFKSFIQMVFSFKQGRHTYIKVLLLLTFLVQCVF
                     IIGNANFQWSQLILLLPYGFAMSVVYLRDEDVEDYRGYLRRQFFLPLAICLGIIAQPA
                     YLYLVQGDLRTDREQVANYIGEQTKDSDKIYVWDNSASIYLSSQRLSAATITTAEPYL
                     NTDDNKNSLMYNINKNEAKFVVVNKNLPILDEIKTNLESQYQSVQTTDYFTIYQKNE"
     gene            14655..16592
                     /gene="mutL"
                     /locus_tag="U1099_000139"
     CDS             14655..16592
                     /gene="mutL"
                     /locus_tag="U1099_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEM4866491.1"
                     /translation="MSHIIELPEILANQIAAGEVIERPASVVKELVENSIDAGASQIE
                     ISVEEAGLKMIQITDNGEGIAPDEVALALRRHATSKIKNQSDLFRIRTLGFRGEALPS
                     IASVSHMIIETATADSAHGLHLEAKGGVIEKEEPVSRPVGTQITVSDLFYNTPARLKY
                     VRSQQAELSHIVDVVNRLSLAHPEMAFALVNEGRELIRTAGTGKLRQAISGIYGIASA
                     KKMVEIEAEDLDFQISGYVSLPELTRANRNYISIFINGRYIKNFLLNRAILEGYGSKL
                     MVGRFPLAVISIEIDPYLADVNVHPTKQEVRISKEKELMTLIREAISQALKEQDLIPD
                     ALENLAQSSTRPKVKAEQGTLPLREPKIYYDTIKQDFFLKPDVVAEDVKPLEEDRQEI
                     VESPAENKPTSVQFAERQSVESEDQEHPNLSAKELAKLADKLDREATSTFPELEYFGQ
                     MHGTYLFAQGKTGLYIIDQHAAQERVKYEYYREKIGQVDNSAQQLLVPYIFEFPQNDA
                     LDLVHKMDALRQVGVNLEEYGSNQFILREHPIWMKEEEIESGIYEMCDMLLLTDQVSI
                     KQYRAELAIMMSCKRSIKANHALDDYSARDLLRQLSYCQNPYNCPHGRPVLVHFSKSD
                     MEKMFRRIQENHTSLRELGKY"
     gene            16631..17221
                     /gene="ruvA"
                     /locus_tag="U1099_000140"
     CDS             16631..17221
                     /gene="ruvA"
                     /locus_tag="U1099_000140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEM4866492.1"
                     /translation="MYDYIKGILTKITAKYIVVETQGVGYILQVANPYAYSGQVQQEV
                     TVYTHQVIREDAHLLYGFATENEKSVFLSLISVSGIGPTTALAIIAVDDNDGLVRAIE
                     QKNITYLTKFPKIGKKTAQQMILDLEGKFVMSEEAGPVQQVAPSSENIALDEAMEAME
                     ALGYRPAELKKIKKFFEGTNDTAENYIKSALKMLMK"
     gene            17475..18044
                     /locus_tag="U1099_000141"
     CDS             17475..18044
                     /locus_tag="U1099_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEM4866493.1"
                     /translation="MSRCAWVNLNNPLYIAYHDEEWGKSLHDEQSLFELLCLESYQAG
                     LSWEIVLNKRQAFRSAFFNYDIQKVAAMTDSELDSLLTNPDIIRHKAKLYATRANAQA
                     FLRVQEEFGTFDTYLWSWVDFTPLDNPIKSFRELPTKSELSERLSKDLKKRGFKFVGP
                     VCIYSYLQAAGLLNEHEETCEIGKKLRTN"
     gene            18081..19262
                     /locus_tag="U1099_000142"
     CDS             18081..19262
                     /locus_tag="U1099_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEM4866494.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKCAELGIDVYFHTAVGD
                     NEQRLLSVLEVARQRSDLVILCGRLGPTEDDLTKQILATFLGRNLVFDELAMAKLDRF
                     FASRPGRVRTPNNERQAQIVEGSQALQNPAGLAVGGMIEQDGVTYIVLPGPPSELKAM
                     FSESLLPLLSQSQQQLYSRILRFFGIGESQLVTVLADLIDKQTDPTLAPYAKVGEVTL
                     RLSTKATSQEEANLRLNQLEEEILQHDKLADYFYAYGEDNSLVKTVATRLAEKRQTIA
                     IVEQGTGGLLQAELSLALADQPYFSGGKVIGQLGTESGRLSEEADCIRQELQADLGLA
                     VSVLIKPESTEDNVLAKVYLTLATTSGISQKEIDLGGYSWQYLRQLACLQAWDFVRNT
                     L"
     gene            19314..20465
                     /gene="recA"
                     /locus_tag="U1099_000143"
     CDS             19314..20465
                     /gene="recA"
                     /locus_tag="U1099_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEM4866495.1"
                     /translation="MAKKPGKKLEDITKKFGDERKKALDDALKSIEKDFGKGAVMRLG
                     ERAEQKVQVMSSGSLSIDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKDGG
                     IAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSV
                     AALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTVAIFINQLREKVGVMFG
                     NPETTPGGRALKFYASVRMDVRGNTQIKGTGDKKDQNVGKETKVKIVKNKVAPPFKEA
                     VVEIMYGEGISRTGELIEIGSNLGIIQKAGAWYSYNGEKIGQGSENAKKFLADNPAIF
                     DEIDRKIRVHYGLIEADGVEEVATEEAPVVAEEIQDVILDLDGGIELED"
     gene            20701..21099
                     /gene="spx"
                     /locus_tag="U1099_000144"
     CDS             20701..21099
                     /gene="spx"
                     /locus_tag="U1099_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEM4866496.1"
                     /translation="MIKIYTVSSCTSCKKAKNWLNAHQLSYNEHNLAKEAITKEEILN
                     ILTKTENGIASIVSSKNRYAKSLDFDIEELSVNEVIDLITSNPRILKSPILIDEKRLQ
                     VGYKEDDIRAFLPRAVRNVENAQARMRAAL"
     gene            21199..21465
                     /locus_tag="U1099_000145"
     CDS             21199..21465
                     /locus_tag="U1099_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEM4866497.1"
                     /translation="MGFTEETVRFRLDDTDKQEISKTLTSVYRSLEEKGYNPINQIIG
                     YVLSGDPAYIPRYNDARNQIRKHERDEIIEELVRYYLKGNGIDL"
     gene            21465..21884
                     /gene="ruvX"
                     /locus_tag="U1099_000146"
     CDS             21465..21884
                     /gene="ruvX"
                     /locus_tag="U1099_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEM4866498.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIPIDEEKGEFGLER
                     LTELVEQYKVDKFVVGLPKNMNNTSGPRVEASQAYGDLLTERYKLPVDYQDERLTTVA
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLDRTF"
     gene            21896..22216
                     /locus_tag="U1099_000147"
     CDS             21896..22216
                     /locus_tag="U1099_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEM4866499.1"
                     /translation="MAHHHDHEHDHNHDERELITLVDDQGNETLFEILLTIDGQEEFG
                     KNYVLLIPASAEEDENGEVEIQAYSYIENENGTEGDLQPIPEDATAEWDMIEEVFNSF
                     MEEE"
     gene            22474..23052
                     /locus_tag="U1099_000148"
     CDS             22474..23052
                     /locus_tag="U1099_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEM4866500.1"
                     /translation="MKDSFTERSKRLSEELERCLLADKNILVILDIMDRLNLSDCWLC
                     AGTIRNFIWNQYSFDEETDVDLVFFDENISYEETMEIESDLYQNYPAYRWELKNQVYM
                     HIHSPNTKPNQSSRDAIEKFPERCTAIGICRTERNKLEIFAPFGLDDIYAYQVRPTPH
                     FLADPNRMELYRQRILKKQWKKRWLSLTVKET"
     gene            23125..23391
                     /locus_tag="U1099_000149"
     CDS             23125..23391
                     /locus_tag="U1099_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM4866501.1"
                     /translation="MEFETVCLKVRNLGRLYCYFKVKFRNHIEAIIRKQESQKCKFDR
                     LFHEDIHSLIQSPSLGNDELLMLWGLLRDYRKNLINDRLENMKN"
     gene            23713..24021
                     /gene="rpsJ"
                     /locus_tag="U1099_000150"
     CDS             23713..24021
                     /gene="rpsJ"
                     /locus_tag="U1099_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEM4866502.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVETATRTGAQVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIINPTQKTVDALMKLALPSGVNVEIKL
                     "
     gene            24234..24860
                     /gene="rplC"
                     /locus_tag="U1099_000151"
     CDS             24234..24860
                     /gene="rplC"
                     /locus_tag="U1099_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEM4866503.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYAAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGQEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGNRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            24885..25508
                     /gene="rplD"
                     /locus_tag="U1099_000152"
     CDS             24885..25508
                     /gene="rplD"
                     /locus_tag="U1099_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEM4866504.1"
                     /translation="MANVTLFDQTGKQAGEVVLNDAIFGIEPNQAVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLP
                     QKVRRLALKSVYSEKVAENKFVAVNSLEFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNIPGVKVATATTASVLDIANADKLLVTQAAISKIEEVLA"
     gene            25508..25807
                     /locus_tag="U1099_000153"
     CDS             25508..25807
                     /locus_tag="U1099_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEM4866505.1"
                     /translation="MNLYDVIKKPVITESSMGQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKTKRVGRYVGRTNKVKKAIITLAADSKAIELFATADAE"
     gene            25825..26658
                     /gene="rplB"
                     /locus_tag="U1099_000154"
     CDS             25825..26658
                     /gene="rplB"
                     /locus_tag="U1099_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEM4866506.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDGVEAIVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTVVHNIELKPGRGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATVGTVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            26760..27041
                     /gene="rpsS"
                     /locus_tag="U1099_000155"
     CDS             26760..27041
                     /gene="rpsS"
                     /locus_tag="U1099_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEM4866507.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            27059..27403
                     /gene="rplV"
                     /locus_tag="U1099_000156"
     CDS             27059..27403
                     /gene="rplV"
                     /locus_tag="U1099_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEM4866508.1"
                     /translation="MAEITSAKATARTVRVSPRKSRLVLDNIRGKSVADAIAILKFTP
                     NKAAGIIEGVLNSAIANAENNFGLEKANLVVSEAFANEGPTLKRFRPRAKGSASPINK
                     RTAHITVVVAEK"
     gene            27416..28069
                     /gene="rpsC"
                     /locus_tag="U1099_000157"
     CDS             27416..28069
                     /gene="rpsC"
                     /locus_tag="U1099_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEM4866509.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRNFIKK
                     ELADASTSTIEIERAVNKVIVSIHTAKPGMVIGKAGSNVDALRAQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGESIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEALTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            28073..28486
                     /gene="rplP"
                     /locus_tag="U1099_000158"
     CDS             28073..28486
                     /gene="rplP"
                     /locus_tag="U1099_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEM4866510.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKQVDFGQYGLQATTSSWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEVAGVSEEIAREAFRLAGHKLPVKVKFVKREAE"
     gene            28496..28702
                     /locus_tag="U1099_000159"
     CDS             28496..28702
                     /locus_tag="U1099_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEM4866511.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFELRFQAAAGQL
                     EQTARLNEVKKQIARIKTVQSETK"
     gene            28726..28986
                     /gene="rpsQ"
                     /locus_tag="U1099_000160"
     CDS             28726..28986
                     /gene="rpsQ"
                     /locus_tag="U1099_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEM4866512.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            29011..29379
                     /gene="rplN"
                     /locus_tag="U1099_000161"
     CDS             29011..29379
                     /gene="rplN"
                     /locus_tag="U1099_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEM4866513.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDIIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRADGSYIKFDENAAVIIREDKNPRGTRIFGPV
                     ARELRDGGFMKIVSLAPEVL"
     gene            29518..29823
                     /gene="rplX"
                     /locus_tag="U1099_000162"
     CDS             29518..29823
                     /gene="rplX"
                     /locus_tag="U1099_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEM4866514.1"
                     /translation="MFVKKGDKVRVIAGKDKGVEAVVVTALPKVNKVIVEGVNIVKKH
                     QKPNSENPQGAIVEKEAPIHVSNVQVFDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            29847..30389
                     /gene="rplE"
                     /locus_tag="U1099_000163"
     CDS             29847..30389
                     /gene="rplE"
                     /locus_tag="U1099_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEM4866515.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGDA
                     VSNAKNLEKAAQELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVTVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGMDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            30404..30589
                     /locus_tag="U1099_000164"
     CDS             30404..30589
                     /locus_tag="U1099_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEM4866516.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RDLAYLGQIPGVTKASW"
     gene            30914..31312
                     /gene="rpsH"
                     /locus_tag="U1099_000165"
     CDS             30914..31312
                     /gene="rpsH"
                     /locus_tag="U1099_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEM4866517.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIATILKNEG
                     FVKNVEFIEDDKQGIIRVFLKYGPNGEKVITNLKRVSKPGLRVYSKREDIPKVLNGLG
                     IAIISTSEGLLTDKQARQKNVGGEVIAYVW"
     gene            31777..32313
                     /gene="rplF"
                     /locus_tag="U1099_000166"
     CDS             31777..32313
                     /gene="rplF"
                     /locus_tag="U1099_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEM4866518.1"
                     /translation="MSRIGNKVITLPAGVELAQNNGVVTVKGPKGELTREFPTAIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTSRANLNNMVVGVSEGFKKELEMRGVGYRAQLAGNK
                     LTLAVGKSHPDEVVAPEGITFEVPTPTQIVVSGINKEVVGQTAAYIRSLRAPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            32402..32758
                     /gene="rplR"
                     /locus_tag="U1099_000167"
     CDS             32402..32758
                     /gene="rplR"
                     /locus_tag="U1099_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEM4866519.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKISGTAARPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALAESARENGLKF"
     gene            32777..33271
                     /gene="rpsE"
                     /locus_tag="U1099_000168"
     CDS             32777..33271
                     /gene="rpsE"
                     /locus_tag="U1099_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEM4866520.1"
                     /translation="MAFKDNAVEIEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVESAKKNMIEVPMVGTTIPHEVRSEFGGARVLLKPASEGS
                     GVAAGGATRAVIELAGIADVTSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            33286..33468
                     /gene="rpmD"
                     /locus_tag="U1099_000169"
     CDS             33286..33468
                     /gene="rpmD"
                     /locus_tag="U1099_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEM4866521.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNPAIL
                     GMVNAISHLVTVEEVK"
     gene            33604..34044
                     /gene="rplO"
                     /locus_tag="U1099_000170"
     CDS             33604..34044
                     /gene="rplO"
                     /locus_tag="U1099_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEM4866522.1"
                     /translation="MKLHELQPATGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRPGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNAFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITSKGGSVEVI"
     gene            34058..35368
                     /gene="secY"
                     /locus_tag="U1099_000171"
     CDS             34058..35368
                     /gene="secY"
                     /locus_tag="U1099_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEM4866523.1"
                     /translation="MFFKLLKDALKVKLVRSKILFTIFILFVFRVGTHITVPGVNAKS
                     LEALSNLPFLNMLSLVSGNAMRNFSVFALGVSPYITASIIVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFVQSIGITAGFNALSGAKLTNMPLNWQTYLLIGAILTT
                     GSIIVTWLGEQISEKGYGNGTSMIIFAGIISSLPGTFHEIYIDRFVNIESSRLGESAI
                     FVAALVVLIFFVVYFTTFVQQAEYKLPIQYTKRAQGAPSSSYLPLKLNPAGVIPVIFA
                     GSITAVPTSLIQYFASQNKSAGWLLTVQEYFDYSTAKGMIVYAGLIIAFTFFYTFVQV
                     NPEKTAESLQKSAAYIHGVRPGNGTEQFLSKLLTRLAVIGALFLSFVALLPILAQNLF
                     GLSSSIAFLGTSLIIVISTSIEGIKQLEGYLLKRKYVGFLEITE"
     gene            35462..36103
                     /locus_tag="U1099_000172"
     CDS             35462..36103
                     /locus_tag="U1099_000172"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEM4866524.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEKFNVAHISTGDMFRAAMANQTE
                     MGILAKSYIDKGDLVPDEVTNGIVKERLVQDDIKEKGFLLDGYPRTIEQAHALDENLA
                     DLGIELQGVINIEIDPSKLVERLSGRIIHKETGETFHKVFNPPVGDYKEEDFYQREDD
                     KPESVKRRLEVNIAQGQPIIDHYRAKGLVHDIEGDQDIDLVFQAIDTVLSKLQ"
     gene            36225..36443
                     /gene="infA"
                     /locus_tag="U1099_000173"
     CDS             36225..36443
                     /gene="infA"
                     /locus_tag="U1099_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEM4866525.1"
                     /translation="MAKEDVIEIEGKVVDTMPNAMFTVELENGHQVLATVSGKIRKNY
                     IRILVGDRVTVELSPYDLTRGRITYRFK"
     gene            36468..36584
                     /gene="rpmJ"
                     /locus_tag="U1099_000174"
     CDS             36468..36584
                     /gene="rpmJ"
                     /locus_tag="U1099_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEM4866526.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            36604..36969
                     /gene="rpsM"
                     /locus_tag="U1099_000175"
     CDS             36604..36969
                     /gene="rpsM"
                     /locus_tag="U1099_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEM4866527.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            36987..37370
                     /gene="rpsK"
                     /locus_tag="U1099_000176"
     CDS             36987..37370
                     /gene="rpsK"
                     /locus_tag="U1099_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEM4866528.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            37415..38353
                     /locus_tag="U1099_000177"
     CDS             37415..38353
                     /locus_tag="U1099_000177"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEM4866529.1"
                     /translation="MIEFEKPTITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSIKIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKKIELDVVGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIADGATFKAVLTVNSGRGYVPAEDNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTLEINTNGTIVPEDALGLSARILMEH
                     LGLFTDLTEVAKSAEVMKEAEVASDDRMLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKVKLADLGLGLKKDK"
     gene            38368..38754
                     /gene="rplQ"
                     /locus_tag="U1099_000178"
     CDS             38368..38754
                     /gene="rplQ"
                     /locus_tag="U1099_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEM4866530.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            39065..39920
                     /locus_tag="U1099_000179"
                     /pseudo
     CDS             39065..39920
                     /locus_tag="U1099_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            40127..40597
                     /locus_tag="U1099_000180"
     CDS             40127..40597
                     /locus_tag="U1099_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4866531.1"
                     /translation="MEFEKVYASVKGIVNKARKEFYIKLWDRDDWEQEGMMTLFELLE
                     AQPWLVDEQVQLYCYFKVKFRNRIKDRIRKQESQKRKFDRMPHEDIHELSHAIQSPGL
                     INDELLMLRGALRDYRKNLSNDQLDKYEKLISGQCFNGRREMIRDLQIHLKDFR"
BASE COUNT        12561 a         7508 c         9074 g        11837 t
ORIGIN      
        1 ttttcaaaaa atgcagacga tttgcgaata agtaagtgag agggaaaatt cccctcaagg
       61 agatacacac ttgaacaaaa taaatcattt tcaaaatgat ttacgaataa acaagtgtag
      121 gaaaaatgaa aggagaagaa ggatgattat agcaccagct ttatggattt tatcatggtg
      181 gaaaaaataa gtttgtttta atttagggga gtagagatga taacagttca acatatccat
      241 aaacagtttg gagacaaggt tgctttagac aacattagct ttgatattca agaaggacaa
      301 atctttggct tcttgggtcc ctctggatcg ggaaagacaa ccatgattaa gattattacc
      361 ggccagctac cagcagatag cggaaaatcc tatctactag gtaaagaatc gaccctattg
      421 tcaccagagg actttgaagc aatgggcttg gtcagtgatt cgagtggttt cttcgagaag
      481 atgaccttgg aaaataattt gttgttctat gcaaaattct ttgatgtcag tccgaaagaa
      541 gtagaggtta ttctgcaaaa agtagggtta ctggatagta aaaagacaat tgcagaaaaa
      601 ctatctacag gtatgaaaca gcgaatgttg cttgccagag ccctgatcaa ttcaccaaaa
      661 gtattgtttc ttgatgagcc gacaagtggt ctagatccaa caacttcaaa acggattcat
      721 gaactattat tggagctaaa aggtaagggg acgacgatct tcttgaccac gcatgatatg
      781 gcagaagcga cattcttatg tgatcaattg gttctattga atcaaggaaa tttggttgaa
      841 aagggaagtc cgcaggattt aattcataag tacaatgtca ctaaaaaagt gagggttact
      901 tttgaggatg gtagtgatac tgagctgttg tttagtgagt tgacacctga gaagttagct
      961 caggcaacca ctattcactc ttgtgagccg actttggaag acatttttat tcaacagaca
     1021 ggagcaacct taaatgtata aacgattttt agccttgctt tggcttcgca cacaagtctt
     1081 ggtgaccaat aaaaacatcc tattgccagt attgatgcct tacttcatgt tattgctgtt
     1141 taagtttgtc atgaaaacag atgataccaa ggcaatggat ttgatgggaa tttgtttttc
     1201 aatggctatt ggtatgtcag taggaagtcc tatatctgca atgatttctg aagaaaagga
     1261 aaagaataat ctgtctacct tgttactgac aggtgttcgc ccaagtgaat atgtgctgtc
     1321 tattttgtgt tatcctgttg tcattagctt tgtcaacctc attctatttc cagctatcac
     1381 acaggccgat gtatcaggat acatgcttag ctactcagtc atcatgctct tgacatcatt
     1441 ggcttctatt ctaatcaacc ttttaatcgg ggcaacttcc caatctcaat ccaagtcaca
     1501 ggtgacctcg atgatgatca caatggcagt atctatggga cctagcctgt ctttgatgaa
     1561 tgagacagtg gggcagattt tgaaatatag ttttctagga gcttatattg aatttttcta
     1621 aaatccccat tttgaacttt tgaatcaaga attctattac ctattggggt ggattgtcct
     1681 gattagtttg gccttgattc tctacttggg aaattggatg aaatatttta agaactccct
     1741 atcaggaata aaagaaaaaa tccaatagtc tttataatat gagtaaatta gaaaggagaa
     1801 atttatggta tcagcattca ttatcggtgt cggtgtagga attattgttt attgtattca
     1861 aaactaatag gaaattgaag aaagagtatt atattatgaa ggaggagaaa cacatgctag
     1921 tagctgcagt tacaggtttc gttgttggtt atgtggcggc acattatttg agaccataat
     1981 tgatggtgaa atatgagtat taaaattctt tggtttttcc agagaatttt tctttttgtt
     2041 tacgaataaa taagtgaggg agttattgct catctatctt attgataaaa aggggaaaca
     2101 atatgaatat gttttggtca atttttaaat cggtaataca tcgaagggat gttgctttgt
     2161 tctatgcttt tgctggttta ccaatcttag ttcctatact gtccaaattt ttggtaggcg
     2221 taaaggctga atatacggat aattttttag attttctagg agcagcattg gcaacacagg
     2281 atgggattgt gttaccaata ttgttgttat ccttgataat ttccgcagtt tttagggatg
     2341 agattgatag cggtattcta tttttatata aagatctgaa tagaactcga ctttttaatg
     2401 caaaaatcat tagtttagtg acgatgtatg cctcttatgt ccttctaaca atgctgacaa
     2461 gtgcgattgc ttattttggc ttcttgaatg cttctggaaa agtagtatcg gatgattgga
     2521 ataatgtaca gtccaccttc ctatctattt ttgcaacaat ttcaatcaat gttatcggta
     2581 ttctattggt tgccatggtt tctatcaaag ctaaatcctt acaggcagtt ttagcaggag
     2641 ttttttggtc gctgtttaca acaactgcac catttctcat tggagttcgt tatgtcgtac
     2701 cgaatggcta tgctcagatg agctttaatc agccactgct tgcatggtcc ttggtagcag
     2761 ctataacaac attatatatt gttgctacct atctcaaagg ccgttcgaac tttgaaaagc
     2821 tagaatttta gaggtgtttt atggtaatac gctatattag accagttgcc ttatcaattg
     2881 ctttgattat tttagcgtca atttcatatc ttctggcaac atctttggtt ttgtttagtc
     2941 ccagtcaatt tttacaggtg gcttacctat tttccattat ggtcggaatg ccagtaggtt
     3001 ttattctatt tccatctatg ttaaccaagc gttatcaact ttgccaggag ctatcggaag
     3061 taaagttcag ttggaaaagt tttgttttgc tggctattgc aattttcctg atcaatttct
     3121 ggtgtattca aagtgatgag tatgtcaatc aatttattat tgcgacttgt gaagaattct
     3181 tgttccgtta tcttatctac cgtattctga aaagtgaata ccctacttgg ctagctatgt
     3241 tggtcacttc tctcttgttt ggagttttgc tacatatgaa ttatccattg ttggataatc
     3301 taatcattcg aactccacta ggtttattgt tctctgtgtt agctacacgt tttggattgc
     3361 agtatgctat cggaggacat tggatctaca atctgttggt ttcgagattt ccattttaat
     3421 tgtgagggtt atgatgatat ttattctact attgagttta ttgtatactt gtttgacagt
     3481 tgtaagttcg caactgctgt taagcctttc ggttcctatt ttgttattgt tgacctattt
     3541 gctaccaccc attgtcaatt caatctgttt aggattgcta aaggataatt cttggaaagc
     3601 atggtcagca ggcatttttc caacaatgtc aatgatattt tatgctgctt ttgcctacat
     3661 tacgagtagt aatggtcagt gggctcagtt ttctcaactg aatacagttt cagacgatgt
     3721 catgagtgtc gaggtagcta gtaatctgtt agctccatca caaatactat ttgttgctgt
     3781 agtgtattat ggtgtagcta ttgctacata ctttattcaa caaacaagtc gtcagaaaga
     3841 aaaaggagtt acttatgctt aaaatcaatc atttatcaaa acagtttgca ggaaacgaat
     3901 tttactcact aaaagatgtg agtttagaga tcaataaggg cgagattgtt gggctgattg
     3961 ggaaaaacgg agctggaaaa tccacgctca tgaaattaat agccaagtcc ctaaaaccaa
     4021 gttcaggaac cattacttat aaggggacgg atatttttag tcaggataat ttgttggcag
     4081 attttgggat tatgattgat cccgtcttct atccagaaat gacagttatg gacaatctta
     4141 agttttattt ggatctgcat gggaaaaaag acctctatcc aaatattgag aagactttga
     4201 aattagtaga attgtgggaa gctcgcaatc ggaaacccaa gggattctcg tttggtatga
     4261 aacaacggac tgctttggca attgccttgg ttgcggagcc agatttcctt atcttagatg
     4321 agccgtttgt gggcttggac ccgattggtg ttcagaaatt gattgatatt ttgaagcagt
     4381 ggtcgagtga gcgacaaatt tccatgctga tttcgagtca ccaactgggg gagctggagg
     4441 ccttgtgcag ccgctatgtc tatatcgaat cgggagaatt ggcagaggct tttgaaggca
     4501 aatctcatcc aagtgtgctc gtgcagttga atacgactaa aaacctagac tctgtagagg
     4561 ctttgctcaa tgaccatgtg gtgctagagg gggaagtgtt agaaatttcc actgctacac
     4621 ctaccagtga gctaaatcag atttttggtg tgctggctcg tcaagaattg attgtgaaac
     4681 tagaagtgaa agagaatcac ttgaaagaaa tctttacgaa agggtagtga gtatgaaaca
     4741 aatttttcca gctatcttta aaacgatttg gaagcgaaag gagacgcaga tttatctcct
     4801 gttcactttg tttcctttta tttatctggt cacttcattt attgaagggt cgaattttat
     4861 gcaaattcat gctagtgagg ggtcggttag ccttgtagcc tttacgaata tgatgatgag
     4921 ctctgtggat agttttattc ttcctagttt gaccctgtat ttcttggcta tctctgtctt
     4981 taggaaggaa gtagatgaac ataccatgtt tttatacaga gatcttgggc ggaaacagat
     5041 tttctggtct aaatatttgg gtctcttagc aacaattgtt attttctatg gtctcttctt
     5101 tgcgacatct gcatttgtac attatgttcg agtaattcac ctgccttttg gcagtccgtc
     5161 cgtttttgat actagtttgg cagagagttt gagtaatgtg ttttgtattg tcgcttattt
     5221 actaaaagat attttatcgg ttagtttggc aactgctctt tgtttgtatc tgaaaaatat
     5281 taccacaatg atcataggat tcttggtgac gattacgatg atgattttag cagttgttgg
     5341 cggtccagtt gcgatgattt ttccaactag ttatatgcct ttagcgagcg aaggtctaac
     5401 aggagtggga cttgcttact taggagctat tggtgtgacg cttgtttacg cactcgtatt
     5461 tactaaaatt gctgcgaaaa aattcaagaa tttggagttc taggtatgaa tagaagactc
     5521 gtaggagtat tgttgaaatc gctcaataaa acgatgttct tgtttggaat agcgctgagt
     5581 gctcttgggg ttggaatggg cttattcctt ccccagttta ttggtcagtt gctagatcaa
     5641 acctatttaa gcaatctatt aacacgacca gaattgctgg ctggcttcat ccttttcttt
     5701 gtatcggttt atacggtaca agccctatcc aattatttca tcgggcgtag tggtggtaac
     5761 gcactcaaac agttgcaaca atatatttat gaatccttgt tgaccacata tgtaaaggac
     5821 ttggatcagt accaatccgg tgatcttgcc agtcgtttga ccaatgatat gtcggttgtt
     5881 ctgaacttca tcaccgttat tcttccaaat ttcttgatga atggtttgat ggttatcggg
     5941 tcaatttatt ttctgtggac catcagtccg tggctcacag gtcttagtct cttgttgctt
     6001 cctatgttgt caatggttat gattccaatg aatcagcggt tggaaggtta ctactctgct
     6061 tatcaagagg gattagggca ggtttctagt agaattagtc ataaatttac aacgattcgt
     6121 ttgatgaagg cctttcaggg agaaaagcat gaacaacgag agatgggcaa gtcttttcag
     6181 aaactgtcgc aaacctttga gagaatgatt agactgtcag cggtgcaaca tactttggtt
     6241 agtagcttga tgacgggttt tatcattctg atgttgctgg tagcaggtat agaagtgacc
     6301 aagggagtga tgaccatggc tacattgaca acctttgtat tgtacatgat gcaactgatt
     6361 gatccagtaa cagatattgc ctcttcctta aatgaactaa cggagtttca ggcggtgtct
     6421 aaacgtttgg tagagttgct ggagctgact aaggaagaac aggcagatac agagttggtt
     6481 gatgaagcta gtatccagtt ggaaaatgtt cggttttcct atcatacaga tccggttttg
     6541 aatggagtat cggttagaat tcctgaagga gctcatgtgg cgattgttgg tccaagtgga
     6601 gcaggaaaat caactatttt tgccttgctg atgaaatact atcaagacta ccaaggagag
     6661 attcggattg gtcaacaatg cttaagtgat atttcaacca aggagatgag aagattgatt
     6721 tcctttatac ctcaagataa taccctcttc catgggacga ttagagaaaa tctattgtat
     6781 ggaaagaacg agtcagtgtc tgaagagcgt attgctcata ttctcaagga gttgggattg
     6841 tctcatttag tagctgagtt ggaagatggt ttagatactc ggatttcaga aaatgggaca
     6901 ggtttgtcgg aaggccaaaa acaacgattt agcattgcga gagccttgtt gttagagcat
     6961 cctatctatt tgttggatga ggcgacagca agtttagata ctgtcacaga acgtgtcatt
     7021 agcaaagcga ttgaccgttt aacagctggg aaaacaaggc taaccattgc tcaccgtttg
     7081 cacacagtgc gtgaggcgga tgcgattctg gtcttagata aaaatgggca agtagccgat
     7141 tatggtcctc accagcagtt agtggagaga aatcatctct atcaagattt cttaagaggc
     7201 ttaccgcaag ccagttagtg aatgggagtc gatggaccga ctagtctaag tctagggtta
     7261 cctaataata aaatggaata ctgatgatta cttgcacaat aaatagatag gagaagagaa
     7321 tgaaacaaac aacttacaca ttatctttgg gaggaaatga tagcggggat tcgaaagttt
     7381 caaagatgga aaaaaagttt gaaaaatata tggaggggat cgcagagcgg ttacggcgtt
     7441 ctgggaagag gagtattgat aaatggttta tccgtgtgcg gattgaggag taaagtagct
     7501 atgaccaaaa ttcacagacg tttttataaa tagtgaaaaa tagaatgaat tctcttttag
     7561 tttcccttaa gtttggcgag ttatacttta ttcaagctag aaatagcgag acttttactg
     7621 aaatagtttc aatatcgtca tccgtaagag ctaaagctca aacggctggc atatcttttc
     7681 aatccgtatc tctaagtcgc aagcgacaag gactagggat tatataatct cctgagaata
     7741 ctggactcac tgagtctggt attttcattt tatgctataa tggtttcatg acaaatcgaa
     7801 ttttagatat ggaacaaatg caggacgagg agtatgttga gcgtaccctg cgtccgcaga
     7861 aattaaatga atacatcggt caggataagg tcaaggacca gctcaaaatc tttatcgagg
     7921 cggccaagct ccgtgatgaa gccttggacc atacccttct ttttggccca ccgggtctgg
     7981 ggaagaccac catggccttt gtcattgcca acgaattggg cgtcaatatc aagcagacca
     8041 gtggtcctgt tattgaaaaa gcgggtgact tggtggccct tctcaacgac ttggagcctg
     8101 gcgacgtcct ctttatcgat gaaatccacc gtatgcccat ggcggtcgag gaaattctct
     8161 actctgctat ggaggatttc tacatcgaca ttatgattgg agcaggggag gccagtcgct
     8221 ccgtgcattt ggagttgcca ccgtttaccc tgattggagc aaccactcgt gcgggtatgc
     8281 tatccaatcc tctgcgggcc cgttttggta ttaccggtca tatggagtat tatgaactgg
     8341 ctgatttgac ggagattgtc gagcgaacag cggacatctt tgacatggag attactcatg
     8401 aagctgctat tgagctggct cgtcggtctc gtggaactcc ccgtatcgcc aaccgcttgc
     8461 tcaagcgggt gcgggatttc gcacagatta tgggagacgg cctgattgat gataacatta
     8521 cggacaaggc cctgaccatg ctggatgtgg accgcgaggg gctggactac gtggaccaga
     8581 agattctccg caccatgatt gagatgtacg gcggcggtcc cgtcggcctc aacactctgt
     8641 cggtcaatat tgccgaggag cgtgagacgg tggaggacat gtacgaacct tacctgattc
     8701 agcagggctt cctcatgcgg acacggacgg ggcgggttgc gacagccaag gcttacgaac
     8761 acctgggcta tccctatacg gaaaaataat ggcagagcag atgagacgag tagctcgttt
     8821 atttggagac tggcctgaaa cgataatctg gacctgtcta gaaggtacta tgggagacat
     8881 ttatgttgat gacagccagt caccccagtc agctctagct ctctatggac ggcagagctt
     8941 ctttggtttt ttagctggcc agccacatcg agacttgcta aaaatctgtg agggaaagaa
     9001 tattattcta gtcccccaaa accaagcctg gtcagacttg atagaagaag tttacggaga
     9061 cggagttcgt ttttttactc gatacgctac gaaaaaggac actgagtttg accttgggca
     9121 tttacagaaa ctggttgatg acttgcctga aagcttcgat atgaaactga ttgaccgtaa
     9181 tctgtacgag acttgtctgg tagaggaatg gtcacgggat ttggtgggaa attatataga
     9241 tgtggaacaa tttttggact tgggtctggg ctgtgtcatc ttgcataagg gacaggtggt
     9301 ctcaggggct tcatcctatg ccagctattc agctgggatt gagatagaag tggatactag
     9361 ggaagactat cgaggtctgg gtttggcaaa agcctgtgcg gctcagttaa ttttagcttg
     9421 tttagaccgc gggctctacc ctagctggga cgcccataca ctgacttcct tgaaacttgc
     9481 ggaaaaactg ggctatgaac tagataaagc ctatcaagca tacgaatgga gatagaatga
     9541 caacaccgac ctttatttgg gatttggatg gaacactttt agattcttac gaggccattt
     9601 tggctggtat ccaagaaacc tatgaacaat ttgacatccc ttttgatcga gaggaagtaa
     9661 gaaatttcat cctccgctat tctgtcaagg atttgctggt gcgtgatgcg gacaagtacg
     9721 gtctggatag cgatgagctc aatcatgtgc gtgcaacttc cttgaaggag aagaatacgc
     9781 agattccctt gatgacaggt gcggtggaaa tcttaaactg gactgcggaa caaggcattc
     9841 aaaattttgt ctatacccac aagagtgaca atgcctttca ggtcttggag gacttgggca
     9901 ttcgccacca tttcacagaa atcttgacca gcgattctgg ctttgcccgc aaaccaagtc
     9961 cagaagctct gctatttctc atcgaaaaat acgggctgga taaggaaagc acctactata
    10021 ttggcgaccg tctgctcgat gtggaaacgg ctattaatgc gggtattcgt agtatcaatc
    10081 tgcaaattaa tggcgtggag cagaattgga agattgtttc tttattggac ataaaacaaa
    10141 tattaacaga taaatcaagt cataacaacc tgtgaaaacg ggtggcttgt acaccagcta
    10201 taagctgttt ctctccagcg atgtctaaag acgtccgctg aacttcgttc aggttagtgc
    10261 tatgattact tgacgaatgc ccgtaacatc gggcttttat cttgttatgg cttcaagcta
    10321 taataaaaac gaatccagcc gtctgtttta agaagagcat gatatggaat ttgtgtaata
    10381 tatttgatat aatttaaaaa gtatatttgg aggtgtggtt tatgaacgat aaggagtttg
    10441 gacagcgtgt gcgtcaatta cgagaatctg ctagtatgac acgtgaacag ttttgtgacg
    10501 atgaactgga actctctgtg cgccaattaa ctcgtattga agcaggtgct tccaagccga
    10561 ctttttcaaa gattcagtat attgcaactc gtttaggtat gggactttac gagcttatgc
    10621 cagattatgt atctttaccc gaaagatatt ccaagctgaa gtttgatgtg cttcgcaccc
    10681 caacttatgg taatgaagat ttggcggaaa agcgagatgc catgatgaca gaaatctatg
    10741 acgattatta tgatgaattg cctgaggagg agaaaatagt tgtagacacc ctatgttccc
    10801 tatttgatgt attggataca gatagccagg aatacggtaa agaaattctt gatgattatt
    10861 tacatcaaag ctatcaccgt tccaaactat ctataaacga tttaatgatt ttgcgattat
    10921 ttattgaaca ttgtcagttg gaggatttat ctgttggaac ttccaattat gctttattta
    10981 cagatttgat agagaaattg cctcaatcaa tctatgatgt acattcggaa tccttattca
    11041 ttgtccgcga tttatttcta gctattgttc gtattttatt ttcgaaggag ttgtataatc
    11101 atattccagt ctatatagaa aaaatagaaa acatcatgga attatcacaa gattttcaga
    11161 agaagccaat tctaaatctt gtgaaatgga aatatgaatt aaaggtacaa cataatcatg
    11221 aaattgctga gaggtactat aatgaagcga ttacatttgc cagcctacta aatcaatttc
    11281 acttgaaaga aaaactgcaa atggaatggg aaaaagatac gcagagtcta aaaagatgac
    11341 atttttgtcc tgttgagaat ctagagttta ttttatacta gattcataaa acaaaaggag
    11401 attatatgtt atgtttcaat tttttaaatt ttttggtggt tttggtcaac ttggtgatga
    11461 gaactggtgg gtaaaataaa taattgaata ctcagcatag agcgtttatt ttatggggat
    11521 ttaagtcgtg gagtacgata ttttcaaaaa gccaacatcc gttaggcttt tttgttatac
    11581 tagacagtat gtaggaggtg gctatgttag aaaattggtt aaacaccaaa caaggtcagg
    11641 tttttcatta caagatggaa aagattgagt atgccctaga actgctaggg aatccccagt
    11701 ttgcagttcc ggtcattcat gttgctggaa ccaatggcaa gggatcgacc attgccttta
    11761 tgcgtcagct ctttcagact cacggcttgc gtgtcggtag ttttgtatct cctcacatgg
    11821 tgagtgtaca cgacaggatt tgtattgaca gccagcccat ttcagaccat gattttcagc
    11881 actatttaca gaaagtctac gacttggagc aggaagtcgc tacacgctat gagcccttcc
    11941 gttattttga ggtcatggtg ctcattatgt tcctctattt cgaagctcaa caacccgatg
    12001 tggcactagt agaggtgggt atcggagggc ttttggatac aaccaacgtc gtggcaccgg
    12061 ctctcagcgt catcacctcc atcggcatgg accatcagga tttactaggc tcgactttag
    12121 gggaaatagc agagcagaaa gcagggatta tcaaggaaaa cgtgcctgtc gtcttaggac
    12181 cactttgtcc agaaaccaca gccatctgtc gccacattgc ccaagacaaa caagcctctg
    12241 tctaccaatt tggtcaggaa ttcacctata aagcaggaca gttcagcaat gcggacttgg
    12301 aactgtcaga attagttctg ggtctggcag gccatcatca agaagagaat gcggccgtag
    12361 ccctacaaac ttttctactc tatatgacca atattcaaaa agacattcaa cctcagttga
    12421 ttcaacaagc ccttgcccaa actagctggc ctggtcgttt ggaattggtt gctcaagagc
    12481 caaaaatcta tttggatgga gcccacaatg tccccgctat cgaacgcttg attgaattta
    12541 ttcaggttca agaagagcct gtcaccattc tcttttcggc ccttcggcgc aaggactttc
    12601 aagaaatgct ggaattattg gacgaaaaat taccacatac tgcccttgta ttaaccagct
    12661 ttgcttatga tggtgccttg tcggaggaaa accgacaagg acgagattat gttgaaaatt
    12721 accggcagtt tatagaagct tggcaatcta gcaagcaagg gattttgatt gtcacaggct
    12781 ctctctactt tatctcagaa gttcgtcgga tttttaaaaa tgaatatctt tttgcgaata
    12841 aataggtatg acacatgcag aaaaagtagc ctttttaaaa gaatttcaag agaacaaacg
    12901 ccataccttt cgccaattag aacagtttgc aaaaatttcc agaactcccg ataggaaatg
    12961 acatcgggag tgctttttga tataataagg aaagcaaatc caaggagaag aaaatgacag
    13021 aattagatgt agtctcggac gtagttactc atcctgagga aacttatcgg cgtagtcgta
    13081 gagcatcacg cagacagcaa gtgcaactga atggtgaatt tcatgaaaga gtattaaaga
    13141 caaagtattg gccagctctt gtttggagct tagttttaag tgtttttagc gtagccaatc
    13201 cactgctaat gccatttgca accaatatgc aaactcaaaa tttatatgca gggatggcaa
    13261 tggcaaatgg acaaatccct tatggtgatt tttttggtac aagcgggtta ttattctatt
    13321 tacttgcttt cttaggtcat ctgggaggaa ctttcattat ctttggaatc ctccagttta
    13381 tagcattact gatagcggga gtctatttct ataaggttgt agcttatttt agtcagtcag
    13441 aacacctagc gacaagttct agtcattggt tctatgtatt tatttttgca cttggttttg
    13501 gagggatgta tgcagaaatg tttgctcttc catttctttt gactagtgtg tggtttctgg
    13561 tccgctattt tgaaaatgca gtgcgtgatg aggcatttat cctctacgga atcgatgcgg
    13621 cattggtatt tttaatttac ccaaaaagtc taatcctttg gttagttgca ggtctagttt
    13681 tatttatttt taatatccaa caccgccaag ttactcgagg aatctatcaa ttattagcga
    13741 ccatttttgg ttttcttctg attctatatg cggtaggtta ttatgctttt gaggcacaga
    13801 ttctggggac agctattcaa caaactttcc tttataactt acagttagac tttcaccatt
    13861 cctatctata tttagccttg gcgattgtgt ctgtattttt actcttatct ggattcttta
    13921 aaagtttcat ccaaatggtg ttttctttca aacaaggaag acatacctat atcaaagttc
    13981 tcctattatt gactttctta gttcaatgtg tctttattat tgggaatgct aattttcaat
    14041 ggagccaact cattcttctt ttaccgtatg gttttgcgat gtcagtcgtt tacctgcgtg
    14101 atgaggatgt agaagattat cgtgggtatt tgcgtcgtca attctttctt ccgttagcta
    14161 tttgcttggg tattatcgct caacctgctt atctttattt ggtacaggga gatttgagaa
    14221 ctgaccgtga acaagttgcc aattacattg gtgaacaaac gaaagattcg gataagattt
    14281 atgtttggga taatagcgca agcatctatc tttcaagtca acgtttatct gccgcaacga
    14341 ttactacagc agaaccctat ctgaatactg acgacaataa aaatagcttg atgtataaca
    14401 tcaataaaaa tgaggcaaaa tttgtagtcg tcaataagaa tttgccaatt ctagatgaaa
    14461 tcaagacaaa tttagaatca cagtaccagt ctgttcagac aacggattac tttacgattt
    14521 atcaaaagaa tgaatgattg ctcaggcagt cattttttct ttagcctatc tgggaaaagg
    14581 agggaaagtg gatatgtggt ataatgtaaa aataagcatt cgggaaaaat tattaaagaa
    14641 atgtaggaga aatgatgtca catattattg aactaccaga aatactagcc aaccagattg
    14701 cggctggtga ggtcattgag cgaccagcca gtgtggttaa ggagttggtg gaaaattcca
    14761 ttgacgcagg ggctagtcag attgaaatta gtgttgaaga ggctggcctt aaaatgattc
    14821 aaattacgga taacggtgag gggattgccc ctgatgaagt agcgcttgcc ctccgccgtc
    14881 acgccaccag taagataaaa aatcaatcgg atttgtttcg tattcgcacc ctcggttttc
    14941 gtggagaagc tctgccttcc attgcttctg tcagtcatat gatcattgag acagctacgg
    15001 cagactctgc acacggactt catttggagg ccaaaggtgg tgtcatcgag aaagaggagc
    15061 cagttagtcg tccagttggt acgcagatta ctgtttcgga tttattttac aatacccctg
    15121 ctcgtctcaa atatgttcgc agtcaacagg ctgaattatc acatattgtt gatgttgtaa
    15181 atcgattgag tctagctcat ccggaaatgg cttttgcctt agtaaacgaa ggacgagaat
    15241 taattagaac tgcagggaca gggaaacttc gtcaggcaat ttcagggatt tatgggattg
    15301 cttctgccaa aaaaatggtt gaaattgagg cagaagatct ggattttcag atttcaggtt
    15361 atgtttcctt gccagagttg actcgtgcca accgcaacta catttccatt ttcatcaatg
    15421 gtcgctatat caagaatttt ttgctgaatc gagcgatttt ggaaggttac ggcagtaagt
    15481 tgatggttgg acgctttccg ttggcagtga tttctataga aattgacccc tatcttgctg
    15541 atgtcaatgt gcatccgacc aagcaggaag ttcgcatctc caaggaaaaa gaactcatga
    15601 ccttgattcg agaggcgatt agtcaagcgc ttaaagagca ggacttgata ccagatgctc
    15661 ttgagaattt agctcagtca agtactcgac caaaagtaaa agcagagcaa ggcaccttac
    15721 cactcagaga gccaaaaatc tattatgata cgattaagca agacttcttc ttaaaaccag
    15781 acgtggtcgc tgaggatgtc aaacctctcg aagaggatag gcaagagatt gttgagtcgc
    15841 ctgccgaaaa taaacctaca tctgttcagt ttgcagaacg tcagtcggtg gaatcagaag
    15901 atcaggagca tcctaatcta agtgcaaaag aattggcaaa actggcagat aaattagacc
    15961 gggaagcgac ctcgacattt ccagagttag aatattttgg tcaaatgcat gggacgtatt
    16021 tatttgcgca aggtaagaca ggactttata tcattgacca gcatgccgca caagagaggg
    16081 tcaaatacga atactaccgt gagaaaattg gacaggttga caattcggct caacagttat
    16141 tggtgcccta tatttttgaa tttccgcaga atgatgccct tgacctagtc cacaaaatgg
    16201 atgcccttcg tcaagttggt gtcaacttgg aagaatatgg atctaatcaa tttattctgc
    16261 gtgaacatcc tatttggatg aaggaagagg agattgagtc tggcatttat gagatgtgcg
    16321 acatgttgct cttgacggat caggtgtcaa tcaagcagta tcgggcagaa ctggctatca
    16381 tgatgtcttg taaacggtca atcaaggcca accatgcttt ggatgattat tcggcgcgtg
    16441 atttattgag acaattgtct tactgccaaa atccctataa ctgcccgcac ggtcgcccag
    16501 tcttagtaca ttttagcaaa tcggatatgg aaaaaatgtt ccgtcgcatt caggaaaatc
    16561 acacgagttt gcgagaattg ggcaagtatt aacatagttg aaaaagttga cgcattagta
    16621 aaggaagtat atgtacgact atattaaagg aattttaaca aaaataactg caaaatatat
    16681 tgtggtagaa acacaaggag taggctatat cttgcaggtt gctaatccct acgcatactc
    16741 tggacaagtc cagcaagaag tgacggtcta tactcatcaa gtgattcgag aagatgctca
    16801 tttgctctac ggatttgcta cagaaaatga aaaatccgtc tttctgagtc tgatttcagt
    16861 atcaggtatt ggtccaacaa cggctctggc tattattgct gttgatgata atgatggact
    16921 tgttcgtgct attgagcaga aaaacattac ctacctgacc aagtttccga agattggcaa
    16981 gaaaacagcc cagcagatga ttttggacct ggaaggcaag tttgtcatga gcgaagaagc
    17041 gggtcctgtt caacaagtag caccatccag tgaaaatatc gctcttgatg aagctatgga
    17101 agccatggaa gcccttggtt accgcccagc cgaactcaag aaaatcaaga aattctttga
    17161 aggcaccaat gacaccgctg aaaactacat caagtcagcc cttaaaatgc tgatgaagta
    17221 ataggatact aaggcgttaa gcgatacaaa gcaaaataga cggccgaaca gaaattttgc
    17281 tagaaatttc gttgtgagac ctccgcaatg acaactaggt aaaaggcgag ttttgacgaa
    17341 gccttttgcc agatgtcaac tgcgttattc ggtgatattt aggattcagt ttgacaattt
    17401 tacaaagtat cgtagccgtg tttcagtcag tttatcctga ttcgcacttt agttattcac
    17461 gaaaaggagt ttctatgtcc cgttgtgctt gggttaatct aaataatcca ctttatattg
    17521 cttaccatga tgaagaatgg ggcaagtcac ttcacgatga gcagtctttg tttgagttgc
    17581 tttgcttgga atcctaccaa gcaggacttt cctgggaaat tgttctcaat aaacgccagg
    17641 cttttcgctc tgcttttttc aactatgata ttcaaaaggt tgcggctatg acagatagcg
    17701 agttagatag ccttctgacc aatccagaca tcattcgtca taaggcaaag ctatacgcta
    17761 cccgcgccaa tgctcaggcc tttcttcgag tacaggagga attcggcacg tttgatacgt
    17821 atctttggtc ttgggttgac tttacacctc ttgacaaccc tattaagtcc tttcgagagc
    17881 taccgaccaa aagtgaacta tcagaaagat tgtccaaaga tttgaagaaa cgaggtttca
    17941 agtttgtagg tcctgtctgt atttattctt atttgcaggc agcaggtctg ttgaatgaac
    18001 atgaagaaac ttgcgagatt ggaaagaaat tacgaactaa ttagatgagc gagcaatcgc
    18061 accctagaca gaggaggaat atgaaagcag aactaatcgc cgttgggacg gagattttga
    18121 caggtcaaat cgttaatacc aatgctcagt tcctttcgga gaaatgtgca gagctgggaa
    18181 ttgatgtcta tttccacaca gctgttggag ataatgaaca gaggcttctg tctgtactgg
    18241 aagtagcccg tcagcggagc gacctggtca ttctctgtgg aagattgggt ccaactgagg
    18301 atgacttgac caagcagatc ctggcgacct tcttaggtag aaatcttgtg tttgatgagt
    18361 tggctatggc aaaattagac cgtttctttg ccagtcgccc aggtcgtgtc cgtacaccaa
    18421 ataatgaacg tcaggcacag attgtggagg gaagtcaggc gctacagaat ccagctggtt
    18481 tagctgttgg tggtatgatt gagcaagatg gtgtaaccta tattgttttg cctggcccac
    18541 caagtgagct caaggccatg ttttctgaga gtctcttacc tttactgtcc caatctcagc
    18601 agcaacttta ttcgcgtatc ctacgttttt ttggcattgg tgaaagccag ttggtgactg
    18661 ttttggcgga cttgatcgac aagcagacag acccaactct tgctccttat gcaaaagttg
    18721 gagaggtgac tttacgcttg tccaccaaag cgaccagcca agaagaggca aatctacgtt
    18781 tgaatcagtt ggaagaagaa atcttacaac atgacaaact ggcagactat ttctatgcct
    18841 acggagagga caatagtttg gtcaaaacgg tagcgactcg tttggcggag aaaagacaaa
    18901 ccatcgctat cgtcgaacag gggacaggtg gtctcttgca agctgaatta agcctggctt
    18961 tggctgatca gccgtatttt agcggaggaa aagtcatcgg tcagctaggg acagaatcgg
    19021 gcaggctatc agaggaagct gactgcattc ggcaggagct gcaagctgat ttgggtttgg
    19081 ctgtgtctgt gcttatcaaa ccggaatcaa cagaggacaa cgtacttgca aaagtatatc
    19141 tcactttggc tacgacctcg ggtatttccc aaaaagagat agatttaggt ggttattcgt
    19201 ggcaatacct tcgccagctt gcttgtctgc aggcctggga ttttgtacga aacactttgt
    19261 gaaatagcag atatttgcta taatagtttg actaagaaaa gaggagaaaa tcattggcta
    19321 agaaaccagg aaaaaaatta gaagatatta caaagaaatt tggcgatgag cgtaaaaaag
    19381 cgctggatga tgccctaaaa tcaattgaaa aagattttgg taagggtgct gttatgcgtc
    19441 ttggtgagcg tgctgaacaa aaagttcaag tcatgagctc aggtagtttg tcaatcgaca
    19501 ttgcgcttgg agcgggtggc tatccgaaag gtcgtatcat tgagatttat ggtccagaga
    19561 gttcaggtaa gacaactgtt gcccttcatg cagtagctca agcccagaaa gatggtggta
    19621 ttgctgcctt tatcgatgcg gaacacgcct tggatccagc ctatgcagca gctcttggtg
    19681 tcaatattga tgagttacta ttgtcacagc cagactcagg ggaacaaggt cttgaaattg
    19741 caggcaagct gatcgactct ggtgcggttg acttggttgt tgttgactcg gttgcagccc
    19801 ttgtaccacg tgcagaaatc gatggcgata ttggtgatag tcatgttggt ctgcaggctc
    19861 gtatgatgag tcaggctatg cgcaaactcg gagcatccat caacaaaact aagacggtag
    19921 ccattttcat taaccagttg cgtgaaaaag tcggtgtcat gtttggtaac cctgaaacaa
    19981 cacctggtgg acgtgcgctt aaattctatg catctgtccg tatggatgtt cgtggaaaca
    20041 cacaaatcaa agggacaggc gacaagaaag atcaaaacgt tggtaaggaa accaaggtga
    20101 agattgtgaa gaacaaggtg gcgccaccat ttaaagaagc tgttgttgaa atcatgtacg
    20161 gtgaaggaat ttctcgcaca ggtgaattga ttgagattgg tagtaacctc ggcatcatcc
    20221 aaaaagcagg tgcttggtat tcatataatg gagaaaaaat tggccaaggt tctgaaaatg
    20281 ctaagaaatt cttagcagat aatccagcaa tctttgatga aattgaccgc aagatacgtg
    20341 ttcattatgg cttgattgaa gcagatggag ttgaagaagt tgcgaccgaa gaagctcctg
    20401 ttgttgcgga agaaatccaa gatgttatcc tagatcttga tggcggtatt gaattagaag
    20461 attaattcct aacaaaatag atttaatatt gaactatttg tgcatatctg ttataggtgt
    20521 gtacgaagtt gtttagtata agaacgaaaa agtctggaga ttccagactt ttttctaata
    20581 aaaaccgaat ttttacccca aaatttaaag tttttttaaa aaataggtct gcagaccgag
    20641 ggaatttatg atataatagt ctataatgtg cttaataaaa tgtagcaaag gagtgactac
    20701 atgattaaaa tttatactgt atctagttgt acttcttgta agaaagcgaa aaattggtta
    20761 aatgctcacc aattatctta taatgagcat aatcttgcga aggaagccat tactaaggaa
    20821 gaaatcctaa acatcttaac aaaaacagag aatggtattg caagtatcgt atcgtcaaaa
    20881 aaccgttatg caaagagtct ggactttgat attgaagagt tgagtgtgaa cgaggtcatt
    20941 gatttaatta cctctaaccc acgtattttg aagagtccaa tcttgattga tgagaaacga
    21001 cttcaagtgg gctataaaga agatgatata cgcgctttct tgccacgtgc agtgcgcaat
    21061 gttgaaaatg ctcaagctcg tatgagagca gctctataag atgtacaaaa ggactctttt
    21121 ggggttcttt ttgcgcattt tttgagaata attaaaattt gtgatataat gtaaaaaata
    21181 gggaaagaag gtgtaagtat gggatttacc gaagaaaccg ttcgttttcg cctagatgat
    21241 acagataaac aagaaatcag caagacattg actagtgttt atcgttcgct agaggaaaag
    21301 ggctataatc cgattaacca aatcattggc tatgtattaa gtggggaccc tgcttatatt
    21361 cctcgctata atgatgcccg caatcagatt cgtaagcatg aacgagatga aatcattgaa
    21421 gaattggtgc gctactattt gaaagggaat gggattgacc tctaatgaga ataatgggat
    21481 tagacgtcgg ttccaagaca gttggtgtag ccatttcaga tccgttaggt ttcacggccc
    21541 aagggttgga aatcatccca atcgatgaag aaaagggcga attcggtctg gagcgtttga
    21601 ccgaacttgt agaacagtac aaggttgata aatttgttgt aggcttgccg aagaatatga
    21661 ataatactag tggtccacgt gtggaagcta gtcaagctta cggtgattta ttgactgagc
    21721 gttacaaact tccagttgat tatcaggatg aacgtttaac aactgttgca gcagaacgga
    21781 tgctgattga gcaagcggat atcagccgag gaaaacgcaa gaaagttatc gataaattag
    21841 cagctcagct gattttacaa aattatttag atagaacatt ttaaaggaga ttctaatggc
    21901 acatcatcat gaccatgaac acgaccacaa ccacgacgaa cgtgaattga ttactttggt
    21961 agacgaccaa ggtaacgaaa cactttttga aatcttattg acgattgacg gtcaagaaga
    22021 gtttggtaag aactatgttc tcttgattcc tgcaagtgca gaagaagatg agaacggaga
    22081 agttgaaatc caagcctact catacattga gaatgagaat ggcacagaag gcgatttgca
    22141 acctatccca gaagacgcaa cagcagaatg ggatatgatt gaagaagtct tcaacagctt
    22201 tatggaagag gaataaaaag gtccagtgga cctttttatc atgagcatga aaacaagaaa
    22261 gcgaattaag gtccggtgga cctttttatc atgagcctga aaacaagaaa gtgaattaag
    22321 gtccagtgga cctttttgtc atgagcctga aaacaagaaa gcgaattaag gtccggtgga
    22381 gtgaagcagt ctgggggaga ctgtttcagc ccgagttaag aaacagcaag gcgaggaatg
    22441 gattatgagc cagaaaatca aaagggaggc cagatgaaag atagttttac agaaagaagt
    22501 aagaggctga gtgaggaatt agagaggtgt ctactggctg ataaaaatat tttggtcata
    22561 ttagacataa tggatagact gaatttatca gattgctggc tctgtgcggg aactatccgt
    22621 aatttcattt ggaatcaata cagttttgat gaagaaacag atgttgatct tgtgtttttt
    22681 gatgaaaata tttcttatga agagacaatg gaaatagaaa gcgatttata tcaaaactat
    22741 ccagcctatc gatgggaatt aaaaaatcaa gtgtatatgc atatccatag tccgaatact
    22801 aaaccgaatc aatcatcaag ggatgcgatt gaaaaatttc cagaaagatg cacagcgata
    22861 ggtatttgtc gaacagaaag aaataaatta gaaatatttg ctccttttgg attggatgac
    22921 atttatgctt accaagtacg gccaacacca catttcttag cggatcctaa tcgcatggaa
    22981 ttgtatcgac aaagaattct taagaaacag tggaaaaaga ggtggctatc gctaacagta
    23041 aaagagactt aactcccacc aagtaagtag gccaaaaaca gtatttttta taaactaaaa
    23101 caatcaacta cgggagatta tgttatggaa tttgaaacgg tttgtttgaa ggttagaaac
    23161 ttaggtaggc tatattgcta cttcaaagtt aaatttagaa accatatcga ggctataatt
    23221 cgtaaacagg aaagtcaaaa atgtaaattt gatcgtctct tccatgaaga tattcatagt
    23281 ttgattcaat caccgagctt gggtaatgat gagttgctga tgttatgggg tctcctgaga
    23341 gattatcgaa aaaatttaat caatgaccga cttgaaaata tgaaaaattg ataagcggac
    23401 agagttttac tggcagtcaa aaaaataatt cgagacttac aaggatactt gaaaaaaacg
    23461 ttgatagtta actcaaataa aaaataagta atgaagaatc ctgaaataaa agttttggga
    23521 ttttttgaat ttttgagtaa aaactcttgc aatggcaagt ggaatctggt agaatgttct
    23581 agtgccgtaa aaaatacggc tgtagctttg atgcaagagg ttgcgacacg ctcggttgca
    23641 ttgccacgca acacgtgttg gttttcttgt ggagctagcc tattatgtta aatagacgaa
    23701 aaggagaaaa agatggcaaa caaaaaaatt cgcatccgct tgaaagcgta cgaacaccgt
    23761 acacttgata cagcagctgc aaaaatcgtt gaaactgcaa cacgtacagg tgctcaagta
    23821 gcaggtccag ttccgcttcc aacagaacgt agcctctaca caatcatccg tgcgactcac
    23881 aagtataaag actctcgtga gcagttcgaa atgcgtacac acaaacgttt gatcgacatc
    23941 attaacccaa ctcaaaaaac agttgatgct ttgatgaaat tggctcttcc aagtggtgta
    24001 aacgtagaaa tcaaattgta atcaagtagc aaggtaaact aaaagcaagt gttcataatt
    24061 gaacacgggc taaactccgt gtgaaaaaga taaactttcc tagaaacaga agtttcttcg
    24121 tcaagtttcc tattttcact ttgagtttga cgccctttgt atcttggttt gagcatgaaa
    24181 aacgctcgtt aaaaactttt taactataaa ataagaaaag gaatattttt ctcatgacaa
    24241 aaggaatctt agggaaaaaa gtgggaatga ctcaaatctt cactgaatct ggtgaattta
    24301 tccctgttac tgtcatcgaa gcaactccaa acgttgttct tcaagtgaaa acagttgaaa
    24361 ctgacggtta tgcagcagtt caagttggtt ttgatgacaa acgtgaagta ttgagcaaca
    24421 aacctgccaa aggccatgta gctaaagcta acacagctcc taagcgcttc attcgtgaat
    24481 tcaaaaacat tgaaggcttg gaagttggac aagaaattac agttgaaact ttcgcagctg
    24541 gtgatgttgt tgatgtaact ggtacatcta aaggtaaagg tttccaaggc gttatcaaac
    24601 gccatggtca atcacgtggt cctatggctc acggttctcg ttaccaccgt cgtccaggtt
    24661 ctatgggtcc tgttgcacct aaccgtgtat tcaaaggtaa aaaccttgca ggtcgcatgg
    24721 gcggcaaccg tgtaacaatt caaaaccttg aagttgtaca agttgttcca gaaaagaacg
    24781 ttatccttat caaaggtaac gtaccaggtg ctaagaaatc tcttatcact atcaagtcag
    24841 cagttaaagc tggtaaataa taaggaaagg ggaaatcagt cgaaatggca aacgtaacat
    24901 tatttgacca aactggtaaa caagctggtg aagtagttct taacgatgcg atctttggta
    24961 tcgagccaaa ccaagcagtt gtatttgatg tgatcatcag ccaacgtgct agccttcgtc
    25021 aaggtactca cgcagttaaa aaccgttcag cagtctcagg tggcggacgc aaaccatggc
    25081 gtcaaaaagg aactggacgt gctcgtcaag gttctatccg ttcaccacaa tggcgtggcg
    25141 gtggcgtagt cttcggacca actccacgtt catacgcgta caaacttcca caaaaagttc
    25201 gtcgcttggc acttaaatct gtttactcag aaaaagttgc tgaaaacaaa tttgtagctg
    25261 ttaactcgct tgaattcaca gctccaaaaa ctgctgaatt tgcaaaagta cttgcagcat
    25321 tgagcattga ttctaaagtc cttgttattc ttgaagaagg caacgaattc gcagctcttt
    25381 ctgctcgtaa catcccagga gttaaagttg caactgcaac aactgcaagc gtacttgaca
    25441 tcgcaaatgc agacaaactt cttgtaactc aagcagctat ctctaaaatt gaggaggttc
    25501 ttgcataatg aatttgtatg atgttatcaa aaaacctgtc atcacagaaa gctcaatggg
    25561 ccaactcgaa gcaggcaagt atgtatttga agttgacact cgtgcgcaca aactcttgat
    25621 caagcaagct gttgaagctg cattcgaggg tgttaaagtt gcaaatgtta acacaatcaa
    25681 cgtgaaacct aaaacaaaac gcgtaggtcg ttatgtaggt cgcacaaaca aagtgaaaaa
    25741 agcaatcatc acattggctg ctgattcaaa agcgatcgaa ttgttcgcta cagctgacgc
    25801 tgaataatca aaggaggaat taacgtgggt attaaagttt ataaaccaac gacaaatggc
    25861 cgtcgtaaca tgacttcttt ggacttcgct gaaatcacaa caagcactcc agaaaaaagc
    25921 ttgcttgttg ctttgaagag caaagctggt cgtaacaata acggtcgcat cactgttcgt
    25981 caccaaggtg gcggtcacaa acgtttctac cgtttggtag acttcaaacg taacaaagat
    26041 ggcgttgaag caatcgttaa aactatcgaa tacgatccaa accgttcagc aaacatcgct
    26101 cttgtacact acacagacgg tgttaaagct tacatcattg ctcctaaagg tcttgaagtt
    26161 ggtcaacgca tcgtttcagg tccagaagca gatatcaaag ttggtaacgc acttccactt
    26221 gcaaacatcc cagtcggtac tgttgttcac aacatcgagt tgaaaccagg tcgcggtggt
    26281 gagttggttc gtgctgctgg tgcatctgca caggttcttg gtcaagaagg taaatacgtt
    26341 cttgttcgcc ttcaatcagg tgaagttcgt atgatccttg gtacttgccg tgctactgtt
    26401 ggtactgtag gtaacgaaca acatggcctt gttaaccttg gtaaagcagg tcgtagccgt
    26461 tggaaaggta tccgtccaac agttcgcggt tctgtaatga accctaacga tcacccacac
    26521 ggtggtggtg aaggtaaagc accagttggt cgtaaagcac catctacacc atggggtaaa
    26581 ccagctcttg gtttgaaaac tcgtaacaag aaagctaaat ctgacaaact tatcgttcgt
    26641 cgtcgcaacc aaaaataatc tattcttggt tgcttagcat tctatatcat ccgccaactc
    26701 ggtagcccgc aaagcgggca agccgctgtg gtacatattt taaaggagaa aacactaaaa
    26761 tgggacgtag tcttaaaaaa ggacctttcg tcgatgagca tttgatgaaa aaagttgaag
    26821 ctcaagcaaa cgacgaaaag aaaaaagtaa ttaaaacttg gtcacgtcgt tcaacgattt
    26881 tcccaagttt catcggttat acaatcgcag tttacgatgg acgtaaacac gtacctgtat
    26941 acattcaaga agacatggta ggtcacaaac ttggtgaatt tgcaccaact cgtacttaca
    27001 aaggtcatgc tgctgacgac aagaaaactc gtcgtaaata atttaggagg aaaacacaat
    27061 ggcagaaatt acttcagcta aagcaactgc tcgcacagta cgtgtttcac ctcgtaaatc
    27121 acgtcttgtc ttggataaca tccgtggcaa aagcgtagca gacgcaatcg caatcttgaa
    27181 attcacacca aacaaagctg caggcattat cgagggagtt ttaaactcag caatcgctaa
    27241 cgctgaaaac aactttggtt tggaaaaagc taacttggta gtcagcgaag cattcgcaaa
    27301 cgaaggacca acgttgaaac gtttccgtcc acgtgcgaaa ggttcagctt caccaattaa
    27361 caaacgcaca gctcacatca ctgtagttgt ggcagagaaa taaggaggta aaaacgtggg
    27421 tcaaaaagta catccaattg gtatgcgtgt tggcatcatc cgtgattggg atgctaaatg
    27481 gtatgctgaa aaagaatacg cggattacct tcatgaagat cttgcaatcc gcaactttat
    27541 caaaaaagaa ttggctgatg cgtcaacatc aacaatcgaa atcgaacgtg ctgtaaataa
    27601 agtaatcgtt tctatccaca ctgctaagcc aggtatggtt atcggtaaag ctggtagcaa
    27661 cgttgatgca cttcgtgctc aattgaacaa attgactggt aagcaagtac acatcaacat
    27721 catcgaaatc aaacaacctg atttggatgc acaccttgtt ggtgagtcaa tcgctcgtca
    27781 attggagcaa cgtgtggcat tccgccgtgc tcaaaaacag gctatccaac gtgcaatgcg
    27841 cgctggtgca aaaggtatca aaacacaagt ttctggtcgt ttgaacggtg ctgatattgc
    27901 ccgtgcagaa ggctactcag aaggaactgt tcctcttcat acacttcgtg cggatatcga
    27961 ctacgcttgg gaagaagctt tgacaactta cggtaaactt ggtgttaaag tatggatcta
    28021 ccgtggtgaa gttcttccag ctcgtaaaaa cactaaagga ggtaaataac aaatgttagt
    28081 acctaaacgt gtaaaacacc gtcgtgaatt ccgtggaaaa atgcgcggtg aagctaaagg
    28141 tggaaaacaa gtagactttg gtcaatacgg tcttcaagca actactagct catggattac
    28201 aaaccgccaa atcgaagctg cccgtatcgc tatgacgcgt tacatgaaac gtggtggtaa
    28261 agtttggatc aagatcttcc cacacaaatc atacactgct aaagctatcg gtgtacgtat
    28321 gggttctggt aaaggtgctc ctgaaggttg ggtagctcca gttaaacgcg gtaaggttat
    28381 gtttgaagta gctggcgttt ctgaagaaat cgctcgcgaa gcattccgcc ttgctggtca
    28441 caaattgcca gttaaagtta aattcgtaaa acgtgaagca gaataaggag aagacatgaa
    28501 acttcaagaa attaaagatt ttgtaaaaga acttcgtggc ctttctcaag aagaacttgc
    28561 taagaaagaa aacgaattga agaaagaact cttcgaactt cgtttccaag ctgctgctgg
    28621 tcaacttgag caaactgctc gtttgaacga agtgaagaaa caaattgcac gtatcaaaac
    28681 tgtgcaatct gaaactaaat aatagattgg gaaaggagaa tttcaatgga acgcaataat
    28741 cgtaaagttc ttgttggacg cgtagtatct gacaaaatgg acaaaacaat cacagttgta
    28801 gttgaaacta aacgtaacca cccagtctat ggtaaacgta ttaactactc taaaaagtac
    28861 aaagctcatg atgaaaacaa tgttgctaaa gaaggcgata tcgttcgtat catggaaact
    28921 cgcccacttt cagctacaaa acgtttccgt cttgtagaag ttgtggaaga ggcagttatc
    28981 atttaatcaa cctgaaagga gaaaattgac atgattcaaa cagaaactcg tttgaaagtt
    29041 gctgacaaca gtggcgcacg tgaaatcttg acaatcaaag ttcttggtgg ttcaggacgt
    29101 aaattcgcga acatcggcga catcatcgtt gcttcagtaa aacaagctac tcctggtggt
    29161 gcggttaaaa aaggtgacgt tgttaaagcc gttatcgttc gtactaagac aggtgctcgt
    29221 cgtgctgatg gttcatacat caaattcgat gagaatgctg cagttatcat ccgtgaagac
    29281 aaaaaccctc gcggaactcg tatctttggc ccagtggcac gcgaattgcg tgatggcggt
    29341 ttcatgaaaa tcgtttcatt ggcaccagaa gtactttaat caaatagttt taggaaattt
    29401 gttacaaact aacgaagtct gtgacgggtt tagtaaacta gaaaacaaac taagtcccct
    29461 ggttaaatca ctaaccaggg tgcccattag ggcgtaagaa attataggag aaatcaaatg
    29521 tttgtaaaaa aaggcgataa agttcgcgta atcgctggta aagacaaagg cgttgaagca
    29581 gttgtcgtaa cagcacttcc aaaagtaaac aaagttattg ttgaaggtgt taacatcgtt
    29641 aagaaacacc aaaaaccaaa cagcgaaaac cctcaaggcg ctatcgttga aaaagaagct
    29701 ccaatccatg tgtcaaacgt tcaagttttt gacaaaaatg gtgttgcagg acgcgttggt
    29761 tacaagtttg tagacggcaa aaaagttcgc tacaacaaaa aatcaggcga agtacttgat
    29821 taatcacgaa ggaaaggaga agtataatgg caaatcgttt aaaagaaaaa tatcttaatg
    29881 aagtagttcc tgctttgact gaacaattta actattcttc agttatggct gtgccaaaag
    29941 ttgataagat cgttttgaac atgggtgttg gtgacgctgt ttctaacgct aaaaaccttg
    30001 agaaagctgc tcaagaattg gctttgatct caggtcaaaa accacttatc actaaagcta
    30061 agaaatcaat cgccggcttc cgtcttcgtg agggtgttgc gatcggtgcg aaagtaactc
    30121 ttcgtggcga acgtatgtat gaattcttgg acaaattggt tacagtttca cttccacgtg
    30181 tacgtgactt ccacggtgta ccaactaagt catttgacgg acgtggtaac tacacacttg
    30241 gtgtgaaaga gcaattgatc ttcccagaaa tcaacttcga cgatgttgat aaaactcgcg
    30301 gtatggatat cgttatcgtt acaactgcta acactgacga agaatcacgt gcattgctta
    30361 ctggccttgg tatgccgttt gcaaaataag agggagagaa taaatggcta aaaaatcaat
    30421 gatcgctaag aacaaacgcc cagctaagtt ctctacacaa gcttacacac gctgtgaaaa
    30481 atgtggacgt ccacactcag tttaccgcaa attcaaattg tgccgtgtat gcttccgcga
    30541 cttggcttac cttggacaaa ttccaggcgt aacaaaagct tcttggtaag ataagatgtg
    30601 agagcgttta gcaaccaacg caaagatagg agattttaca acgaagttta cttctaggat
    30661 ttgctaagct tgtagctcga actcaattaa aacatgatac taagggcgtg acggacaaag
    30721 tgaaaatagg aactctagcg aagaacgctt gcgttctaga tagagttatc tttttcacac
    30781 aggccgtagc ccgagttcaa atgttaggat gcaatagcat catactagca aattcaaact
    30841 ttgcattgcc tatcaggcac acattaacta gtaagtgatc taatcaaact gctagtaaga
    30901 ggagaaacaa caaatggtta tgactgaccc aattgcagat tttttgacac gtattcgtaa
    30961 cgctaaccaa gcaaaacacg aagtgcttga agtacctgca tcaaacatca aaaaaggtat
    31021 tgctacaatc cttaaaaacg aaggttttgt aaaaaacgtt gaattcatcg aagatgacaa
    31081 acaaggcatc atccgtgtat tcttgaaata cggaccaaac ggtgaaaaag ttatcacaaa
    31141 cttgaaacgc gtttcaaaac caggtcttcg tgtttactca aaacgtgaag atattcctaa
    31201 agttcttaac ggacttggta tcgcaatcat ctcaacatca gaaggtcttt tgactgacaa
    31261 acaagctcgt caaaagaacg ttggtggtga ggttatcgca tacgtttggt aatcttttag
    31321 cttctaattt cgtcatgtgc ctgtgttaga gcgtcttgac atactcaagt atggtctgcg
    31381 tcgctctgcc ttgtcacatt tagaaattga aacctaaaag catgatacaa atgcgagagg
    31441 accagttctt cgtgcgtgtt caaatctgac ttactgatta tttggtatca tgcaaccaaa
    31501 ctagaacaag attcactaat tgaacacgcc tacaactctt tgaaaaaagt gaaaagtgtt
    31561 tcagaaatgc tcaccttgtt cgtcgtaaac gtctgaactt tctctggcct tcggtcagga
    31621 aagtcctagt cgttctgacg agggtgcgtc tgcgaaatca aagacttcgg acttaccgct
    31681 tatttttctt cgagttgctt aacggctttg tatcttgttc accccgtgaa aactagtcgc
    31741 tcgacggctt gataatttaa caggagaatt aaaaatatgt cacgtattgg taataaagta
    31801 attacattgc ctgctggcgt tgagcttgct caaaacaacg gcgtggtaac tgtaaaagga
    31861 cctaaagggg aattgactcg tgaattccca actgctattg aaatccgtgt ggaaggtaca
    31921 gaagttactc ttcaccgtcc aaacgattca aaagaaatga agactattca cggtactagc
    31981 cgtgctaacc tcaacaacat ggttgttggt gtttctgaag gcttcaaaaa agaacttgaa
    32041 atgcgtggtg tcggttaccg tgctcaattg gctggtaaca aattgacact tgctgttggt
    32101 aaatcacatc cagatgaagt ggttgcacca gaaggtatta catttgaagt tccaacacca
    32161 acacaaatcg tcgtgtctgg tatcaacaaa gaagttgttg gtcaaacagc agcttacatc
    32221 cgtagccttc gcgctcctga gccatacaaa ggtaaaggta tccgctacgt tggtgaattc
    32281 gttcgccgta aagaaggtaa aacaggtaaa taatagcttg ctgaggtggc ttagccacct
    32341 ggctgactat ttctcaaatt gtttcgcaag aaacagaatc atacattaag aggtgaatat
    32401 tgtgatttca aaaccagata aaaacaaaat ccgccaaaaa cgccaccgtc gcgttcgcgg
    32461 taaaatctct ggaactgctg ctcgcccacg tttgaacatt ttccgttcta atacaggcat
    32521 ctacgctcaa gtgattgatg acgtagcggg tgtaacgctc gcaagcgctt ctactcttga
    32581 taaagaagtt tcaaaaggta ctaagacaga acaagctgtt gttgtaggta aactcgttgc
    32641 tgaacgcgcg gtagctaaag gtatttctga agtggtcttt gaccgcggtg gatatctcta
    32701 tcacggacgt gtgaaagctt tggctgaatc agctcgtgaa aacggattga aattctaata
    32761 gggaggacac taagaaatgg cattcaaaga taacgcagtt gaaattgaag aacgcgtagt
    32821 agccatcaac cgtgttacaa aagttgttaa aggtggacgt cgtcttcgtt ttgcagctct
    32881 tgtggttgtt ggtgaccgta acggtcgcgt aggtttcggt actggtaaag ctcaagaagt
    32941 accagaagct atccgtaaag cagttgaatc tgctaagaaa aacatgattg aagtaccaat
    33001 ggttggtaca acaatccctc acgaagttcg ctcagaattt ggcggcgctc gtgtattgtt
    33061 gaaacctgct tcagaaggtt ctggggttgc tgccggtggt gcaactcgtg ccgtaatcga
    33121 attggcaggt atcgcagatg tgacttctaa gtcacttggt tcaaacacac caatcaacat
    33181 cgttcgcgca acagttgaag gtttgaaaca attgaaacgt gctgaagaag tggctgcact
    33241 tcgtggcatc tcagtttctg atttagcata agaaaggaga tactcatggc tcaaattaaa
    33301 atcactttga ctaagtctcc aatcggtcgc aaaccagaac aacgtaaaac agttgttgca
    33361 cttggacttg gtaaattgaa ctcttcagtt gttaaagaag ataacccagc tattcttgga
    33421 atggtgaacg ctatctctca cttggtaact gtagaagaag ttaaataatc taacaaatag
    33481 actaagtcgc atagagaaat ctttgcgact tctagtccat tttacatatt tacatacagc
    33541 gagtttgtgg aggaagactc ttgtacttca caggcgctag catataaaag aggagaaata
    33601 ataatgaaac ttcatgaatt acaacctgct acaggttctc gtaaagtccg caaccgtgta
    33661 ggtcgtggta catcatcagg taacggtaaa acatctggcc gtggtcaaaa aggtcaaaaa
    33721 gctcgtagcg gtggcggtgt tcgcccaggt tttgaaggtg gacaaactcc attgttccgt
    33781 cgtcttccaa aacgtggatt tacaaatatc aacgctaaag agtacgcaat cgttaacctt
    33841 gatcaattga acgcttttga agatggtgca gaagtaacac cagttgtgct tatcgaagct
    33901 ggaatcgtta aagctgaaaa atcaggaatt aaaattcttg gtaacggtga attgacgaag
    33961 aaattgacag ttaaagctgc taaattctct aaatcagctg aagaagcaat cacttctaaa
    34021 ggtggctcag tagaagtcat ctaagaggtg gcagcctatg ttttttaaac ttttaaaaga
    34081 tgccttaaag gtaaaattgg tacgtagtaa aattctattt actatcttta tcctttttgt
    34141 cttccgcgta ggaacccaca ttacagtacc aggagtaaac gcaaagagtc ttgaggcctt
    34201 gtcaaacctt ccgttcttga acatgttgag tttggtttca ggaaatgcta tgcgtaattt
    34261 ctcagttttc gcactaggtg ttagtcctta tattacagct tccatcattg ttcaactttt
    34321 acaaatggat attttaccta aatttgttga atggggtaag caaggtgaag ttggtcgtcg
    34381 taaactaaat caagcaacac gctacatttc tttggtactg gcatttgttc aatcaattgg
    34441 tattacagca ggctttaatg ctttgtccgg tgcaaaattg accaatatgc cattaaattg
    34501 gcaaacatat ttgttgattg gagcaatttt gacaacaggt tcgattattg taacctggct
    34561 gggggaacag atttctgaaa agggctatgg taatggtaca tcaatgatca tctttgcagg
    34621 gattatttca tcgttaccag gaacttttca cgagatttac attgatcgct ttgttaatat
    34681 tgaatcaagt cgtttggggg aatcagcaat ctttgttgct gcactcgtcg tattaatatt
    34741 ctttgttgtg tattttacaa cttttgtaca acaagcagaa tataaattac caattcaata
    34801 tacaaaacgt gcacaaggag caccttctag ctcatattta ccgctgaaat tgaatccagc
    34861 aggagttatt cccgtaatct ttgcaggctc aatcactgca gttccaactt ctttgattca
    34921 atatttcgca agtcaaaata agagtgccgg gtggttattg acggttcagg aatactttga
    34981 ttattcaact gctaaaggta tgattgtata tgcaggttta attattgcct ttacattctt
    35041 ctacaccttt gttcaagtaa atcctgagaa gactgcagaa agccttcaga aaagtgcagc
    35101 ctatatccat ggagttcgtc ctggtaatgg cactgaacag tttttgtcaa aattattgac
    35161 aagattggca gtaatcggtg cactcttctt gagttttgta gctttgctgc ctatccttgc
    35221 gcaaaatctc tttgggcttt cttcaagcat tgcgttcctt ggtacaagtt tgattatcgt
    35281 aatctctaca agtatcgaag gcatcaagca attagaaggc taccttctta agagaaaata
    35341 tgtaggtttc ttagaaatta cagaatagaa tataggttga cctagtcaac cttctatttt
    35401 gtttttagat aagtttgtca ataatggcaa atttatgtga aaacaaaata aggagtttgt
    35461 catgaatctt ttaattatgg gtttaccagg tgctggtaaa gggacacaag cggccaagat
    35521 tgttgagaaa ttcaatgttg ctcatatttc aacaggagat atgttccgtg cagcaatggc
    35581 caatcagact gaaatgggta ttcttgccaa gtcgtacatt gataagggtg acctcgttcc
    35641 cgatgaagtt actaatggca ttgtcaaaga acgtttggtt caggatgaca tcaaggaaaa
    35701 aggctttttg cttgatggct atcctcgtac gattgaacaa gcccatgcct tggatgaaaa
    35761 tttggctgac ttaggaattg agttgcaagg tgttatcaat attgagattg atccttcaaa
    35821 attggtagag cgtctcagcg gtcgtattat tcataaagaa actggagaga ctttccacaa
    35881 agtattcaat ccacctgttg gagactacaa agaggaagac ttctatcaac gtgaagatga
    35941 caagccagaa tctgtcaaac gtcgtttaga ggtcaatatc gcacaaggac agccaatcat
    36001 tgatcattat cgtgcaaaag ggctggtcca tgatattgaa ggtgaccaag acattgacct
    36061 tgttttccaa gcaattgata cagtactatc aaaattgcaa taaactaata gatttggctt
    36121 gcatttttta tacaaagatg ataaaatagc ctagtctgac ttataattgt tacctctgtg
    36181 ttcagaggac atcaaatcga tatttagaga ggggttactt ttgcatggca aaagaagatg
    36241 tgattgaaat tgaaggcaaa gtagtcgata caatgccaaa tgctatgttt actgttgagt
    36301 tggagaatgg acaccaagtc cttgcaactg tttcaggtaa aatccgtaaa aactacattc
    36361 gtattttggt cggtgaccgc gtaactgttg agcttagtcc atacgacttg acacgtggac
    36421 gtatcacata ccgctttaaa tagtcgaaat acttggaggg attaaacatg aaagtaagac
    36481 catcggtcaa accaatttgc gaatactgca aagttattcg tcgtaatggt cgtgttatgg
    36541 tgatttgccc agcaaaccct aaacacaagc aacgtcaagg ttaagaaata gaaaggagaa
    36601 aacatggctc gtattgctgg agttgacatt ccaaatgaca aacgtgtagt tatttcatta
    36661 acttatgttt atggtatcgg tcttgcaaca tctaaaaaaa ttcttgcagc tgcaggtatt
    36721 tcagaagatg tacgcgtgaa agatttgaca tcagatcaag aagatgctat tcgtcgtgaa
    36781 gttgatgcaa tcaaagttga aggtgacctc cgtcgtgaag ttaacttgaa catcaaacgt
    36841 ttgatggaaa tcggttcata ccgtggtatc cgtcaccgtc gcggacttcc tgtccgtgga
    36901 caaaacacta aaaacaatgc ccgcactcgt aaaggtaaag ctgttgcgat tgcaggtaag
    36961 aaaaaataat ataggaggta gaaaaattgg ctaaaccaac acgtaaacgt cgtgtgaaaa
    37021 agaatatcga atctggtatt gctcatattc acgctacatt taataacact attgttatga
    37081 ttactgatgt gcatggtaac gctattgctt ggtcatcagc tggtgctctt ggtttcaaag
    37141 gttctcgtaa atctacacca ttcgcggctc aaatggcttc agaagctgct gctaaatctg
    37201 cacaagaaca cggtcttaaa acagttgaag ttaccgttaa aggcccaggt tcaggtcgtg
    37261 agtctgctat ccgcgctctt gctgccgctg gtcttgaagt aacagcaatt cgtgatgtga
    37321 ctcctgtacc acacaatggt gctcgtcctc caaaacgtcg ccgtgtataa tcatacattc
    37381 catacacagc ttttcgttta agagggagta agaaatgatt gagtttgaaa aaccaacaat
    37441 aacaaaaatt gatgaaaata aagattacgg cagatttgta atcgaaccat tagaacgtgg
    37501 ttacggtaca actcttggta actctcttcg tcgtgtactt cttgcctcac ttccaggtgc
    37561 tgcagtgaca tcgattaaaa ttgatggtgt actccacgaa ttcgacacag ttccaggtgt
    37621 tcgtgaagat gttatgcaaa ttattcttaa catcaaaggc attgctgtaa aatcttatgt
    37681 cgaagacgaa aagaaaattg aacttgatgt agtaggtcca gctgaagtaa cagcaggaga
    37741 cattcttaca gatagtgaca ttgaaattgt aaaccctgac cattatctct ttaccattgc
    37801 tgatggtgcc acttttaaag ctgttttgac tgtcaattca ggtcgtggtt atgtaccagc
    37861 tgaggataat aagaaagatg atgcaccagt gggaacactt gctgtagatt ctatctatac
    37921 gccagtgaag aaagtcaatt atcaagttga accagctcgt gttggtagca acgatggttt
    37981 tgacaaatta acacttgaaa tcaacacaaa tggcaccatt gttccagaag atgctttagg
    38041 tctttctgca cgtattttga tggagcactt aggtctcttc actgacttga ctgaagttgc
    38101 aaaatctgca gaagtgatga aagaagctga agtggcttca gatgatcgta tgcttgatcg
    38161 tacgattgaa gaattggatc tatctgttcg ctcatataac tgtctgaaac gtgcaggcat
    38221 taacactgta ttcgacttga cagagaaaac tgagccagaa atgatgaaag tgcgcaatct
    38281 tggtcgcaag agtcttgaag aagttaaagt taaattggct gatcttggtc taggattgaa
    38341 aaaagataaa taatatagga ggaaatcatg gcataccgta aactaggacg cactagctca
    38401 caacgtaaag caatgttgcg cgatttgaca actgaccttt tgatcaacga atcaatcgtt
    38461 acaactgaag ctcgtgctaa agaaatccgt aaaacagttg aaaaaatgat cacacttggt
    38521 aaacgtggtg atttgcacgc acgtcgtcaa gcagcagcat ttgttcgtaa cgaaattgca
    38581 tctgaaaact atgatgaagc aactgataag tacacttcta ctacagcact tcaaaaattg
    38641 ttctctgaaa ttgcacctcg ttatgcagaa cgtaatggtg gatatactcg tatcctcaaa
    38701 actgaaccac gtcgtggtga tgctgcacca atggcaatta tcgaacttgt ataagatcat
    38761 caattttgtt gagtgttatg atgatggaat cctattctga ttcttagtct agctctggtc
    38821 taccgctgag cttatctcag cggtaacact catcatgttg attggacgct tgtttacgaa
    38881 attactctaa gatagaataa tttcgtaagc aggcgttttt taatagatga tacatatcag
    38941 aaacagagca tttacaagaa gaacgaatca atagttactc gtggtgctag ttcatttcaa
    39001 aatacaataa aaagcccaaa tggactatac tgtaagtgac gaaacataca ggaggttagt
    39061 ccaaatgagc cacttacagt ataccgctaa atctcatcat ttgcaatgga atgtacgcca
    39121 attatcacaa atttgtcatc acttctacaa caactattgc ccggattcct tcaaatatcg
    39181 gcgaaatgtt ggcttagtta aagtttcaga tgaatcaatc cttgtcttac ttttgttaca
    39241 agctgaacta ggtatgacat cacagcgcca tttctacacc atctgccacc tcttcccttg
    39301 cggtcagtta ctagaaagaa gccgtttcaa tcgtcggtca aaacaattga tttggctcgt
    39361 ccaatcaatc cgaaaagcta tgagtgaaat gcttccatct ggtaaactag ccattataga
    39421 tagttttcct ttgccaacct gttcgcaatc acagagcaac catttttaaa ggattagccg
    39481 atattggcta caacgcctct aaacatcttt ggttctacgg cttcaaagtt catatgctag
    39541 tgactttatc tggttatatt ttgaactatg ttgtcacgcc agcctctgtc catgatatca
    39601 aggtggttta tgagctatta gaggggtgta agcaatcggt tatcttaggt gatctaggct
    39661 acctaagcag tgagctcaaa aaggacttag agcaagaagg ttaccatcta tggacgccgt
    39721 ttcgtaaaaa catgactggg tccgaagaac ataacaattg gaaagtaatg gccatgagac
    39781 gaactgttga aacacgtttt tctgaactgt gtcgtctctt tgacatcgaa catacattag
    39841 ctagaagcct agctggacta cagttacgaa ttgaacaaat tattctagct cataacttac
    39901 gctattttga aatgaactag caccacgggt aatagttatt tatcacaatc ttagaactat
    39961 accaaatcac tcccttctga agcagaatgc aatgtttgcc atagagtatc aatagcaaat
    40021 atacttaaaa aaagaaaaat caaataaagt atgctagaca aaaatgtcct gtagaaagac
    40081 agcacttttc gataaactta aaccatcaac aaaaggagat taagtaatgg aattcgaaaa
    40141 agtgtacgca agcgtcaaag gtattgtaaa taaggctcga aaagagtttt acattaaact
    40201 atgggatcga gatgattggg aacaagaagg aatgatgacc ttgtttgaat tgttggaagc
    40261 tcaaccgtgg ctagttgatg aacaagttca attatattgt tattttaaag tcaagttcag
    40321 aaatcgaatc aaggatcgta tccgcaaaca ggaaagtcaa aaacgcaagt ttgaccgtat
    40381 gccacatgaa gatattcacg aattatctca cgcaatacaa tcaccgggat taataaacga
    40441 tgaactatta atgctaagag gtgccttgag agattatcga aaaaatctga gtaatgatca
    40501 acttgataaa tacgaaaaat taattagcgg acaatgtttt aatggtcgcc gtgaaatgat
    40561 acgtgattta caaattcatt tgaaagactt tcgctaaagc tagtcactaa catcatgtta
    40621 atcgaattaa gaaattgggc agaagcacca taaaaagttc tgttcagttt ctttttttat
    40681 aggttatagc aaagagaaga aggaataggg ggaaaaaaac aaatttcaag ttaaaattag
    40741 ccatcctatc ttatgaagag taggggtgtg actaaatgtg atactcagcc aatagtaacc
    40801 taagtaattg aaagactgat tcagcggtat tatttagaat taaccttatg ctttagtatc
    40861 tacatagaac tttcagtgta aaaaaatccc aaaaatcggt tgacaatgta tataagtcgt
    40921 gatagaataa atgagttgtc tcttgaggga ctggttaaca gaggaacgaa aaaaagtttc
//
LOCUS       DASFSW010000004        35583 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.004, whole genome shotgun sequence.
ACCESSION   DASFSW010000004 DASFSW010000000
VERSION     DASFSW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35583
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.004"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            121..462
                     /locus_tag="U1099_000181"
     CDS             121..462
                     /locus_tag="U1099_000181"
                     /inference="COORDINATES: protein motif:HMM:NF019021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEM4866532.1"
                     /translation="MAVLHHDQIQIGDDWTVKLFEFDPEDYKGKVHGWQREAPNEVNE
                     ILKAINAIAKPRHQAILIMSYILPEKIRSAKQAQRLGIAASTYYLAKNEALKEFAGQY
                     RDGSLLQYLDS"
     gene            880..2133
                     /locus_tag="U1099_000182"
     CDS             880..2133
                     /locus_tag="U1099_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM4866533.1"
                     /translation="MKLQEGVDLHFIDTDQFTTNRIRIRFAAEMSEATVAGRVLVANI
                     FEMGNQEFQTAQAVRRRLAELYGAQFSTSVSKRGRVHCVDVTISYVSPRHLPENEDIT
                     VEILDFLYTCIFRPLKKGRGFDSQIFEVEKTNLINFLQSEIEDNFYHADVEMSKLFYK
                     DPSLQIPRVGRLDLVEKETAESTFQIYRNMLRMDKIDIFVLGKVDREQVKRKLEDFGF
                     TYRNPKLELEYNQEYSNITQEKIERKQARQSILELAYHLQVVYNDVNYPALMVFNGLL
                     GAFSHSKLFMNVREKESLAYTIGSQVSIFSGMLKVYAGISHENRLRVMKLISKQLLDL
                     KCGKFTEEELELTKNMLIHSATLAQDRQNNLIEQVYNQVTLGNRNLSWLDWIEAIKSV
                     SIEDVIRVGQMINLQAVYFMEGTEE"
     gene            2130..3413
                     /locus_tag="U1099_000183"
     CDS             2130..3413
                     /locus_tag="U1099_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM4866534.1"
                     /translation="MKLVEKKYPYMKESVFYGKVDNGLTVILLPKVDFHETYGILTTN
                     FGGIHTRFTLANGKSVVYPAGIAHFLEHKLFETENEEDIMNEFAKLGASANAYTSFRQ
                     TSYLFSTTQKVLESLSLLQSFVREPYFTEDNVEREQGIIEQEIEMYQDDADYRLFTGI
                     LSSLYPESPLAYDIAGTVESIAAITADDLHENFEVFYHPSNMNLFVIGNFDLEAVWKQ
                     ISSYQVAQMDNPAQSFELAGVQKLPIQEHLSEQFEVSTPKLAVGLRGNDEVDKETIQK
                     YRLSLQFLFAMLFGWTSKRYQQLYEQGKIDSSFQFQLEVTPEYHYLIISGDTQEPITL
                     SSILMKALRKFEDDADVTEDHLQLLKNEMYGDFIRSLNSLEFTASHFVSQYSEYENVF
                     DIPQMIQSLQLDDIREAGRRFIDHCDMTDFTIFPK"
     gene            3495..4352
                     /locus_tag="U1099_000184"
     CDS             3495..4352
                     /locus_tag="U1099_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM4866535.1"
                     /translation="MRQKSIGEVLRTAREGRSWTFVDLQRITKIQAKYLQALEYNDFD
                     FIANSDDVQSILKAYAEALELDADVLLDAYETNSLVKYYEDGEEELRSSELKRSYKVR
                     KRKKNSYLPLIYLLLATGLIIIFVTYIVHSRLQNRASITPTTTSYSIVEQSVSEASSG
                     TETSSTEQAQSNSTSSSSSVTENVTISGSGSNIVATLKTVTYPVEVTVTAKNTTSWIS
                     LSDTLLEGGVTLTPDNPTVTTTVEEGVTSTTLILGVVKGVEVTVGGQKLNLSALTADT
                     GSITINFEQ"
     gene            4362..4901
                     /gene="pgsA"
                     /locus_tag="U1099_000185"
     CDS             4362..4901
                     /gene="pgsA"
                     /locus_tag="U1099_000185"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEM4866536.1"
                     /translation="MKKENIPNALTIGRILVIPIFILLLTVWDSTISHVLAALIFALA
                     SITDYLDGYLARKWQVVTNFGKFADPMADKILVMTAFIMLVELGFAPAWVVAIIICRE
                     LAVTGLRLLLVENGGTVLAAAMPGKIKTFSQMFAVIFLLLHWNAVGQITLYIALFFTL
                     YSGYDYFKGASFLFKDTFK"
     gene            4898..5734
                     /locus_tag="U1099_000186"
     CDS             4898..5734
                     /locus_tag="U1099_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEM4866537.1"
                     /translation="MMNIIEVKNLKYKYNQEDERYTLNDVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGDIYIDGDALTIDNVWDKRRLIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGIPLEEMRSRVDEALELVGMTDFKTREPARLSGGQKQRVAIAGVVALRPK
                     IIILDEATSMLDPEGRLDLIKIVREIKERHGMTVISITHDLDEVALSDRVIVMKNGQV
                     ESISTPNELFMREDLVDLDLDRPFTTELASSLRQTGLDLPLRYFTEEELEETLWELIS
                     KK"
     gene            5710..6552
                     /locus_tag="U1099_000187"
     CDS             5710..6552
                     /locus_tag="U1099_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEM4866538.1"
                     /translation="MGIDLQEVSYTYQAGTPFEGRALFGVDLEIVDGSYTALIGHTGS
                     GKSTILQLLNGLNVPTEGRVVIDDVVITATSENKDIKQVRKKVGLVFQFPESQVFDET
                     VLKDVAFGPQNFGVSQEEAETIAREKLALVGIDESLFERSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPAGRQELMDIFKKLHESGMTIVLVTHLMDDVANYADFVYIM
                     EKGKLVRSGKPVDIFQEVDFLESIQLGVPKITKFAANLERRGFVLERLPITIEEFTGM
                     VTHG"
     gene            6545..7339
                     /locus_tag="U1099_000188"
     CDS             6545..7339
                     /locus_tag="U1099_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM4866539.1"
                     /translation="MDKLILGRYIPGDSIIHRLDPRSKLLAMFGFLLMIFWANNLVTN
                     ALLIAFVLGMVVLSRIRLSFFINGLKPMIGIILFTTFFQVFFTPGATILWEFWIFKLS
                     VEGLQQAAIIFVRFVLIIFFSTLLTLTTTPLSLADGIESGLAPLKKFKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQRARGVDFNEGNLIQKVKSVIPILIPLFASSFKRADAL
                     ATAMEARGYQGGDGRTKYRILEWKLADTLAILVMLALAGLLFILKK"
     gene            7471..8025
                     /locus_tag="U1099_000189"
     CDS             7471..8025
                     /locus_tag="U1099_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM4866540.1"
                     /translation="MNITLKKNLKTTIAGLVAGISLLTAGVVSAETYTVKSGDTLSEI
                     AETYNTTVEKLAEQNKITNLDFIHVGQVIELGDVTVKAVESSIEQTQSTSTSTATVTT
                     STYSSNLSAEDAAAKEWIAMKESSGSYDARNGIYIGRYQLTNTYLNGDYSPENQERVA
                     DAYVASRYGSWSAAKAFWLANGWY"
     gene            complement(8482..9354)
                     /gene="sdaAA"
                     /locus_tag="U1099_000190"
     CDS             complement(8482..9354)
                     /gene="sdaAA"
                     /locus_tag="U1099_000190"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEM4866541.1"
                     /translation="MFYTIEELVQQADNFSANVAELMIATEIELTGRSREEILTIMSR
                     NLTVMKASIVDGLTESKSVSGLTGGDAAKLDAYMKSGKTLSDSAVLSAARNAMAVNEL
                     NAKMGLVCATPTAGSAGCLPAVLGVAIEKLNLTEEQQLDFLFTAGAFGLVIANNASIS
                     GAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAICFVIKNMLGLICDPVAGLVEVP
                     CVKRNAMGASYAMVAADMALAGIESKIPVDEVINAMYQVGAALPTAFRETAEGGLAAT
                     PTGRRLTQEIFGED"
     gene            complement(9367..10038)
                     /gene="sdaAB"
                     /locus_tag="U1099_000191"
     CDS             complement(9367..10038)
                     /gene="sdaAB"
                     /locus_tag="U1099_000191"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEM4866542.1"
                     /translation="MTNLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFGDEPT
                     EVEFQLYNSFAKTYRGHGTDVALVAGILGMDTDDPRIPDSLDIARERGIKVYWRVNKD
                     SNTPHPNTTRIIIKNDKKSISATGVSIGGGNIQVTELNGFSVNLNMNTPTIIIVHQDV
                     PGMIAKVTDVLSKYDINIAQMNVTREKAGEKAIMIIEVDARQCENSIAEIEKIPHLHN
                     VTFFN"
     gene            10253..11377
                     /gene="mnmA"
                     /locus_tag="U1099_000192"
     CDS             10253..11377
                     /gene="mnmA"
                     /locus_tag="U1099_000192"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEM4866543.1"
                     /translation="MSIDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDD
                     TDENGFCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDV
                     MCNKEIKFKAFLDYAMNLGADYVATGHYAQVSRDEDGTVHMLRGADNGKDQTYFLSQL
                     SQEQLQKTMFPLGHLQKPQVREIAERAGLATAKKKDSTGICFIGEKNFKEFLGQYLPA
                     QPGRMMTVDGRDMGEHTGLMYYTIGQRGGLGIGGQIGGDNEPWFVVGKDLSKNILYVG
                     QGFYHESLMSTSLQASQVHFTRDMPEEFKLECTAKFRYRQPDSQVTVKVKGDKAEVIF
                     AEPQRAITPGQAVVFYDGQECLGGGMIDMAYKDGEACQYI"
     gene            complement(11420..11809)
                     /locus_tag="U1099_000193"
     CDS             complement(11420..11809)
                     /locus_tag="U1099_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEM4866544.1"
                     /translation="MSTNLEIFNAYNNALIAGDFAALFETMADDIIWHQPGNHSTSGA
                     KIGKEVLGAHLATFAEKTNGTFKVVTNWVSDNDTLVAANVTFLGTRTDGEELNMNGID
                     LFRMEDGKIKEVWLFSADQTAEDAFWG"
     gene            11935..12261
                     /locus_tag="U1099_000194"
     CDS             11935..12261
                     /locus_tag="U1099_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4866545.1"
                     /translation="MKKVSEYGICPFATTQKVLTGKWGLVIIYQLSTGTKRFNELQRL
                     IPGITQTMLTRHLRQLEEDRIISRTVYAEVPPRVEYSLTEMGEKFRSVLDAVEAWGLE
                     YIETLT"
     gene            12403..12864
                     /locus_tag="U1099_000195"
     CDS             12403..12864
                     /locus_tag="U1099_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEM4866546.1"
                     /translation="MDFRTKVDNQIFGVRATALLIKDGKIFLTKDSKGRYYTIGGAVE
                     VNEVAADAVVREVKEELGIDSHVNQLAFVVENKFTQEGIHFHNIEFHFIVEPIGEIPE
                     EMIEDNLKQACEWIDVDKLVNLDVVPVFLAEKLPNWSGQVTHIMNMKEKTT"
     gene            12861..14777
                     /gene="mnmG"
                     /locus_tag="U1099_000196"
     CDS             12861..14777
                     /gene="mnmG"
                     /locus_tag="U1099_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEM4866547.1"
                     /translation="MTHTFAENYDVIVIGAGHAGVEAGLAASRMGCKTLLATINLDMV
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQMKMLNMGKGPAVRALRAQAD
                     KAEYAAEMKRTVERQENLTLRQTMIDEILVEDGKVIGVRTATNQKFSAKAVVVTTGTA
                     LRGEIIIGDLKYSSGPNNSLASITLADNLKELGLEIGRFKTGTPPRVNARTINYEETE
                     IQPDDEKPNHFSFLSKDEDYLQDQIPCWLTYTNATSHEIINSNLHRAPMFSGIVKGIG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTDEIYVQGLSTSLPEDVQQDLIHSIKGL
                     ENAQMMRTGYAIEYDMVMPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIVAGI
                     NAALKVQGKPELILKRSDGYIGVMIDDLVTKGTVEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRQVGLVDDERWQVFQIHKNQFDNEMKRLESIKLKPIKETNEKVVAMGFKPLTD
                     ALTAKEFMRRPDVTYADAVAFIGPAAEDLDAKTIELIETEVKYEGYIAKALDQVEKMK
                     RMEEKRIPADIDWDDIDSIATEARQKFKLISPETIGQASRISGVNPADISILMVYLEG
                     RSRSISKNKSKDSH"
     gene            14884..16845
                     /locus_tag="U1099_000197"
     CDS             14884..16845
                     /locus_tag="U1099_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEM4866548.1"
                     /translation="MKRFRFSTIHFVMIGVILFGLVALVHRFFPIGASTVFALLISLL
                     VLIALFIYQKHSYEFSELEQIEYLNNQANSGLMMLLDKMPVGVIKVRPDSNQVEWFNP
                     FAELIFAQENGEFDQKKLREIIDVGLDEERIYANFSGKRYAVNVDFDQGLFYFFDAST
                     EYSATNSLIGSRPVIGIISVDNYDELEDAVTDSQISQVNSFLAQFVSEFCHDYGIYYR
                     RGTMDRFYFFTDYAVLEKLIENKFSVIDKFREEAKKLDLGLTLSMGLAYGNENHHQIG
                     QVALQNLNMAEVRGGDQVVVKENDESKQPLFFGGGSASSVKRTRTRTRAMMTAVSDKL
                     KSVDAVFVVGHRNLDMDALGSAVGMQYFAQNIMNNAYTVYNPDEMAPDIARAIKKLSD
                     ENCSNLLTVEEAMKMVTFQSLLIMVDHSKIGLTLDKDFYDQFTQVVVVDHHRRDTDFP
                     NNAVLTYIESGASSASELVTELIQFQNDKHHKLNRIQSSLLMAGIMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSLEIQQIAATDFEEYRQINELILSGQRIEANVVIACADDTKEY
                     DNIIPSKAANTILDMAGIEAVFVVTRNTKGYVSISARSHSKINVQRIMEEMGGGGHFN
                     LAAAQVYDQTIAEVCEKLTDKIREEINGN"
     gene            16856..17308
                     /gene="rplI"
                     /locus_tag="U1099_000198"
     CDS             16856..17308
                     /gene="rplI"
                     /locus_tag="U1099_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEM4866549.1"
                     /translation="MKVIFLADVKGQGKKGEIKEVPTGYAQNFLIKKNLAKEATNQAI
                     SALRGQEKSKEKAHAEMIAEAEAIKAKLAEEATLVKFVEKVGPDGRTFGSITSKKIAE
                     ELDKQFGIKIDKRHIQLDHPIRAIGLIDVPVKIYQDVIGIINLSIKEA"
     gene            17340..18695
                     /gene="dnaB"
                     /locus_tag="U1099_000199"
     CDS             17340..18695
                     /gene="dnaB"
                     /locus_tag="U1099_000199"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEM4866550.1"
                     /translation="MAELDELRVQPQDILAEQSVLGAIFLDEGKLVFVREYIEAEDFF
                     KHAHKLIFKAMIALSDRNEAIDATTMRNYLVNHGDLENIGGLAYLAEIISSVPTAANA
                     EFYAKIVAEKSNLRKLIARLTDAVNQAYEGADGSDDIIANAEKALIDVSEGASRSGFK
                     RIDDVLNINFDNLETRAKQTSDITGIATGYPALDAMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLGLTVAIFSLEMGAESLVDRMLASEGVINSRAIRTGHLTQEEWNKY
                     MVAQANLARASIYIDDTPGIKITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGEQEDGGIPNNTVEVIIEKNRSGARGTVELMFQKEYNKFASISKRED
                     G"
     gene            18699..18950
                     /locus_tag="U1099_000200"
     CDS             18699..18950
                     /locus_tag="U1099_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866551.1"
                     /translation="MSDAFTDVAKMKQIKQDIKDHEGQIVELTLENGRKREKNKQGRI
                     VEVYQSLFIVEFEDPNNTFVESYTYSDILTEKILIHYLD"
     gene            19161..19772
                     /gene="rpsD"
                     /locus_tag="U1099_000201"
     CDS             19161..19772
                     /gene="rpsD"
                     /locus_tag="U1099_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEM4866552.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKQGTLGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVEVGQVISVREKSIKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            19839..20288
                     /locus_tag="U1099_000202"
     CDS             19839..20288
                     /locus_tag="U1099_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEM4866553.1"
                     /translation="MWRKYFLLVLLAILVVGGAFFLSQSAATPNESDYVKAVKSYQAI
                     SIDEVEQKVQDEEEFILYIGRETCPYCRDFVPKLTEAVERSRATIYYLDSESDPNGKI
                     QQFRQSQGLATVPSLTYYKSGKLSGILRKGSQATLSEIENFLALQGQ"
     gene            20421..21938
                     /locus_tag="U1099_000203"
     CDS             20421..21938
                     /locus_tag="U1099_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEM4866554.1"
                     /translation="MKKKAWLGLLMSLLAVLFLVACGGKSEKTATSSSSTTSSSSEEA
                     VSGASEKVYTDPSELKDSYDVIVVGSGGAGMSAAISAKDAGASVALLEKMPVIGGNTA
                     KSSAGMNASQTKFQEAEGIADTNDKFYEETLKGGKGTNDPELLRYLVDNSASAIDWLD
                     GMGITLSNLTTTGGMSEKRTHRPADGSAVGGYLVNGLYHNLVERKVPIFVNADVTKLQ
                     DKDGAVTGVEVKIAGETKQISAKAVVLATGGFGADLEMVAKLNPALKGYVTTNQEGST
                     GDGIALAESEGAATVDMEQIQIHPSVEQETSYLITEAVRGEGAILVNSKGERFVNELE
                     TRDKVSAAINSLDPNYAYVIFDDALKDRVKAIAQYEEKGLVKTGATLADLAKEIDLPA
                     DTLQTTLDTWNKAVADKNDAAFGRTSGMDHALNTGSYHAIKVAPGVHHTMGGVKINTN
                     TEVLKTDGSAISGLYAAGEVTGGVHGQNRIGGNAVADIIVFGRQAGEQAAGFAKK"
     gene            22024..23388
                     /locus_tag="U1099_000204"
     CDS             22024..23388
                     /locus_tag="U1099_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM4866555.1"
                     /translation="MKKKIVLLLGAIAVIVLTWSAIAGQRQQSYAEAELRDQDKIWII
                     TDLHYLSQDLFDDGEAFSYIEKTAAGKDLRYGKERMEALVEQVGREHPSLLLVSGDLT
                     LNGEKQSMVELAQYFTEIEEQGTEVLVIPGNHDIASGWARAFKGDQQIVTDQVTAQQF
                     AELFAHHGYQQASSRDKASLSYLAKPFSNAWFLMIDSNIYSDGYGKGAPPTNGRINKE
                     TLEWIEIQLQAAKETGVSLIPVVHHNVLQQHAMLSKGYTLDNAADLKTLFNQYGIQFG
                     FSGHTHSQNIVKEDLGQVNYTEVVNGAFSIYPAIIGQLSLDDTSIHYQKTQLDMASWA
                     DKHQPSDPNLQDHVTYLQTVFDNTSDIMVHNMLNDEGWYDGETADAISQFIMPANRAY
                     FSGEKLDQTWLDEKVFPSQAYQTLQAASPRSFLADYLDVIIKRSQNRDVEKLVLKTEK
                     DFVQ"
     gene            23552..24565
                     /locus_tag="U1099_000205"
     CDS             23552..24565
                     /locus_tag="U1099_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4866556.1"
                     /translation="MTLLSIAIPSYNSEAYLHYCVHSLVMGGDKVEILIINDGSSDRT
                     QEIAEELANQFSNVRAIYQENKGHGGAVNTGIREAKGKYFKVVDSDDWVDTRAYLKIL
                     ESLQALEDENTPVDAFISNFVYEKEGQSRKKSMSYQSTLPENRIFGWEDVGAFSKGQY
                     MMMHSLIYRTDLLREVGLVLPEHTFYVDNIFVFTPLQAVKTMFYLPVDFYRYFIGRND
                     QSVNESVMIKRIDQQLKVNRILVDNLDLEAIDNPDLRSYLLNHVEITTIISCALLNRA
                     GTAEHMMKKQELWHYIRDNNPALFKIVRKGLLGQLTNLYGYPGRKIFNAVYKIAKRIY
                     GFN"
     gene            24635..25546
                     /locus_tag="U1099_000206"
     CDS             24635..25546
                     /locus_tag="U1099_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM4866557.1"
                     /translation="MQRLGQTGLEVSRIGLGCMRMASLTEQETAKVLDTVVGQGINFF
                     DHADIYGGGESEIRFAQGAKLAGLKREDLLLQSKCGIRKGYFDFSKDYILESVDGILK
                     RLDTEYLDVLALHRPDALMEAEEVAEAFYLLKKAGKVHHFGVSNQNIYQMELLQSYFD
                     QPLVVNQLQLSPAHTPLIDAGLHVNMKDDAATMRDGGLIDYCQLKKITIQAWSPFLID
                     LQRGIFANHPNYALLNQTIGEIAERYNVSHETIVVAWILRHPAKIQTIVGSMNPERLK
                     KIAQASQIILTRPEWYDIYRSAGNILP"
     gene            25556..26008
                     /locus_tag="U1099_000207"
     CDS             25556..26008
                     /locus_tag="U1099_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866558.1"
                     /translation="MQWEIKAFDQLSLQELYTILTLRTDVFVVEQACPYPEIDGKDPS
                     CLHLLGTDNGELVAYLRILPAGLSYDEVSIGRVVIKPSHRGKGLGRPMMEQAIAYITT
                     EWKESQIKIGAQAYLEKFYQSLGFEPISEVYLEDDIPHLDMLYRKLVV"
     gene            26103..27854
                     /gene="aspS"
                     /locus_tag="U1099_000208"
     CDS             26103..27854
                     /gene="aspS"
                     /locus_tag="U1099_000208"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEM4866559.1"
                     /translation="MKRSMYAGRVRKEHVGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPESVEAEVMAKAESLRSEFVIEVTGTVVEREQANDNIPTGAVELQVTSLTV
                     LNTAKTTPFEIKDGIEASDDTRLRYRYLDLRRPEMLNNFKLRAAVTHSIRNYLDELEF
                     IDVETPMLTKSTPEGARDYLVPSRVSKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLNEVEIQDIVEGLIAKVLKDTKGIDVTLPFPRMAY
                     DHAMNFYGSDKPDTRFEMLLQDLTELVKEVDFKVFSEAPVVKAIVVKGAADCYSRKDI
                     DKLTDYAKQFGAKGLAWVKVDKGELAGPVAKFLTGITENLTASLQLEDKDLVLFVADE
                     LEVANNTLGALRNRLAKEQGLIDESKFNFLWIVDWPMFEWSEEEGRYMSAHHPFTLPT
                     EETAHHLDGDLAQVRAVAYDIVLNGYELGGGSLRINQKDMQERMFKALGFSAEDAHEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEDNIREVIAFPKNNKASDPMTQAPSTV
                     ASAQLEELALNITLENE"
     gene            27847..28779
                     /locus_tag="U1099_000209"
     CDS             27847..28779
                     /locus_tag="U1099_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM4866560.1"
                     /translation="MNKFFRKQRVRFLRKARKNKFWSVILGISREKYAERISGSIIYG
                     LLSSIAVNFFFRPGNVYSSGVTGLAQIVSALAASYAGWNVPIAVVYYGLNIPLLILAW
                     YKIGYKFTIFTFITVSFSSFFIQFMPPVTLTTDPLVNAIFGGLMLGTGIGFALRNNVS
                     SGGTDIVSIIIRKKTGRQVGSISLIVNIMIMLIAGMTFGWQYALYSMVALFISSRMTD
                     AIFVKQKRMQAMIITNHPERVIKKIHKKLNRGVTIIDGAEGAYNHEQKTILITIITRA
                     EFNDFKHIMKKADPKAFVSVADNVNIIGRFVESD"
     gene            28856..29308
                     /gene="nrdI"
                     /locus_tag="U1099_000210"
     CDS             28856..29308
                     /gene="nrdI"
                     /locus_tag="U1099_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM4866561.1"
                     /translation="MKKKIYLVYISLSGNTESFVKRLKSFFQFQTDWEIELVHVKDLV
                     KQDIPFYQLDAPFVAFLPTYLEGGNGVDNGDVEILTNPLGDFIALGTNAELCLGVVGS
                     GNRNFNNQYCLTAKQYAERFNFPVIDTFELRGMQNDIERIGRKIMELM"
     gene            complement(29462..31513)
                     /gene="pulA"
                     /locus_tag="U1099_000211"
     CDS             complement(29462..31513)
                     /gene="pulA"
                     /locus_tag="U1099_000211"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEM4866562.1"
                     /translation="MALRQFQASLDDVATIRLVMEKRFDSEHMSFSLESNDATSQLFI
                     HSCLEVDNLVIYYLTSLHALTLDKDYTIYDQDRNKTELGYGHIVRSTLFEQSYTYNGQ
                     DLGATYRPEATTFKLWAPISKQVFLVLEGNPHAMTKGAKGVWEITVEGDLEAHSYHYL
                     HKVNSEWISVHDPYALSSTANSGDSYVINPDKLHKIRRAKTQRPISQAIIYEMSVRDF
                     SWQKEAGFKHRSQFLGLTESPTMDGMKLGMDYIRELGVTHIQLLPVYDFGSVDENKPQ
                     AVYNWGYDPMQYNLPEGSYSSQPNDPYARILELQEAIQAYHDADISVIMDVVYNHVYH
                     AEKYAFELIVPGYFYRYDDHGMRTDGTFCGNDVASERSMVRNYIKQSLRQWLELYGFD
                     GFRFDLMGILDSETINQIQIELSAIHPNVYLYGEGWKMATGLEFEKLAHQYNAEQLPE
                     ISFFNDDYRDTFKKLLLNPNRLVDKQLHEKVQHLLTGSRLSHFLTPQQSLNYIECHDN
                     ATAFDYFHIENPNWTPQQQKRAASFGLQLILISQGMAFIHSGQEFFRTKDEIDNTYNV
                     PDRINRLDWTRAIRYKEHIEFIQELITFRKNNPILSQDNYESIQETCDFYWLTEYVLR
                     YQVTSEEKTIQFIINFADSDFTYEREKDKAVLYNYPPVEERDQQEITIAGQSICILED
                     K"
     gene            complement(31537..32520)
                     /locus_tag="U1099_000212"
     CDS             complement(31537..32520)
                     /locus_tag="U1099_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM4866563.1"
                     /translation="MRATIKDVAKLAGVSPSTVTRVIQNSSAISQKTKDLVRKAMADL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDVFYQNPFFPTALRGISQVAADHNYAIQIST
                     GKNEEQRLEAISQMVYGKRVDGLIFLYSKPDDPLVQLAIQHKFPFLILGKADSPFISL
                     VDNDNIQAGFEATNYFINKGYKNIAFVAGNKELVVSQDRYTGYKNALKSHNIPLDENK
                     VKFVSGFLLEDSAYKISKKLLKQDLDAIVTTDTSVAEGVVKYLNEVGVKLPIISFDSV
                     KPKLDIEAYVDVHAIKLGRVAFNTLHQIINDNKEDKQVCYRRVIPHTITEL"
     gene            complement(32530..33312)
                     /locus_tag="U1099_000213"
     CDS             complement(32530..33312)
                     /locus_tag="U1099_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866564.1"
                     /translation="MLPYPFSYFSSIFSPRNMFANRRLMNLWQRLFTTLFLVALLLIP
                     SSLQTVNLETYPLENFVEGVYAPLTDQVVDDLRQYAVIENNHLTYTGNTNWEQVTFGD
                     TVSSSSDFTYQFANQSLTIRKGSDILSELTYEGLNTDDFASKETLTATISKKWYQANR
                     AAVGLSITLISTFILTTKLLFILLGASFFLFLTKKSRFFHFETFAECYNFALNCLGLP
                     TLIACLIGLFGQPVTTLLTIQNILFVLCLLWVFFKTKFRDEI"
     gene            complement(33372..34214)
                     /locus_tag="U1099_000214"
     CDS             complement(33372..34214)
                     /locus_tag="U1099_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM4866565.1"
                     /translation="MKVSVKTRRILNQTLTYLYLIVLSVIIIYPLLITIMSAFKSGNV
                     VAFKLDSNINFTLDNFSKLFSETLYGTWYFNTLIIALLTMVIQTSIVVLAGYAYSRYN
                     FIARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNQSWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPISLDESAKLDGAGHFRRFWQIVLPLVRPMIAVQALWAFMGPFGDYILSKF
                     LLREKEFYTVAVGLQTFISDVKNMKIAYFAAGAILIALPISILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(34216..35511)
                     /locus_tag="U1099_000215"
     CDS             complement(34216..35511)
                     /locus_tag="U1099_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM4866566.1"
                     /translation="MTMKQNPGKALLLSLIPGLGQIYNKQKAKGYIFLGVTLAFLVYF
                     IAIAAGELGNLITLGSVRGQDNSLFMLIRGSFHLIITVVYLAFYALNLKDARDTAKRW
                     NSGMPVATTLKEMVKGIYENGFPYLLIIPSYIAMTFAIIFPVVVTLFIAFTNYDFKHL
                     PPSALLDWIGITNFTNIWKLSTFRTAFGSVLGWTIIWALCASTAQIVIGILTAIIANQ
                     PFIKGKRIFGVIFLLPWAVPAFVTILTFSNMFNDSVGAINTQVLPFLSKFLPFIDNFL
                     IPWKTDPFWTKVALIMMQAWLGFPYIYVLTLGILQSIPNDLYEAAYIDGASAIQKFRN
                     ITLPMILAVAAPTLISQYTFNFNNFSIIYLFNNGGPGSVGGGAGSTDILISWIYKLTT
                     GTSPQYSMAAAVTLIISMIVISISMIAFKKLNAFEMEDV"
BASE COUNT        10431 a         6365 c         8304 g        10483 t
ORIGIN      
        1 gcacgcttgt actgaccgtg atgtaattaa ctctaataat attatatcac agcggagggc
       61 gctgacttgt gcaaacaact agcaaaaaga aaactaaagg agtttccccg ctggtgcagg
      121 gtggcagtcc tacatcatga ccaaatacag ataggtgatg attggactgt aaagctgttt
      181 gagtttgacc ctgaagacta caagggcaag gtacacggct ggcaacgtga ggcaccaaac
      241 gaggtcaacg agattttgaa agctatcaac gcgatagcta aaccaagaca tcaggctata
      301 cttatcatga gttatatatt gcccgaaaag atacgatcag caaagcaggc acaacgactt
      361 gggatagcag catctactta ctacttggct aaaaatgaag ctttgaaaga gttcgccggt
      421 cagtaccgag atggctcact attgcagtac ttggatagtt aagtttgaaa tcattcgtaa
      481 atacccccca actttttttg aacggggtgc tatatcgttc gaattccaca acgccgccct
      541 cttccgtgta caattttccc tttatgaaac ttttggatac agtaaaaaca tggtataata
      601 cacttatcag caatcaaaaa aagcgtaccg attcgatacg ctagacttgc ctgctgaact
      661 cattattttt agtctatcat gctataaatg ataggctttt tttgttcccc tttttgtaca
      721 ctttctaggg aaatgtaacg ctgtataaag tttacacttt ttttcaaaaa tccagtaaaa
      781 tcaactaaaa aggcgtgtaa agcaaccaaa tgctaaagcc aagcatacct tatcacaaaa
      841 tggtataatg gaaggtacaa tgtttgaaag aggaagacga tgaaattaca agagggagta
      901 gatcttcatt ttattgatac agatcagttt acgacaaatc gtatacgtat tcgctttgca
      961 gctgaaatga gtgaggctac agttgctggt cgtgtgttag ttgcaaatat ttttgaaatg
     1021 ggtaaccaag aatttcagac tgctcaggct gttcggagaa gattggcaga attgtatggt
     1081 gctcagttct cgacctcagt ttcgaaacgt ggtagggtgc actgtgtaga tgtgacaatt
     1141 tcatatgtca gtcctcgtca cttaccagaa aatgaggata ttacagtgga gattcttgat
     1201 tttttataca catgtatatt tagaccactg aaaaaggggc gaggatttga tagccagatt
     1261 ttcgaggttg aaaaaacgaa tttaatcaat tttcttcagt cagagataga agataatttt
     1321 tatcatgcag atgttgaaat gagtaagctt ttttataaag atccctctct tcaaattcca
     1381 cgcgtcggta ggcttgattt ggttgaaaaa gaaacagcag aatcaacttt tcagatttat
     1441 cggaatatgt tgcgtatgga taaaattgat atatttgtct tagggaaggt tgacagagaa
     1501 caagtcaaaa gaaaacttga agattttggt tttacttata gaaatccaaa attagagtta
     1561 gaatataatc aggaatactc aaacatcacg caagaaaaaa tcgagcgtaa acaggcaagg
     1621 cagtccattt tggaattggc atatcattta caagtagttt acaacgatgt aaactatccg
     1681 gctttgatgg tatttaatgg tctactgggt gctttctccc attcgaagtt atttatgaat
     1741 gttcgtgaga aagaaagttt ggcctataca attggcagtc aggtttctat tttttcagga
     1801 atgctgaagg tctatgctgg aattagccat gaaaacagac tcagggtaat gaagttaatt
     1861 agtaaacaac tacttgattt aaaatgtggt aagtttacag aagaagaatt agagttgaca
     1921 aaaaacatgt tgattcattc agcaaccttg gctcaagata ggcagaataa tttgatagaa
     1981 caagtatata atcaagttac cttaggaaat agaaatttaa gttggttaga ttggattgag
     2041 gctatcaaat cggtatcaat agaggatgtc attcgagtag gacagatgat taatttacag
     2101 gctgtttact ttatggaggg aacagaagaa tgaagttagt tgagaagaag tatccttaca
     2161 tgaaagaatc tgtcttttat gggaaagttg acaatgggtt gacagttatt ttgcttccta
     2221 aagtagattt ccatgagaca tatggaattc taacgacaaa ttttgggggt attcatactc
     2281 gatttacatt ggctaatggt aaaagtgttg tttatcctgc agggattgca cattttttag
     2341 aacataagct gtttgaaact gagaatgaag aggatattat gaatgagttt gctaaattgg
     2401 gagcaagcgc aaatgcttat accagtttta gacagactag ttacttattt tcaacgactc
     2461 agaaagtatt ggagtctcta tcacttttac aatcttttgt tcgagaaccg tattttacag
     2521 aagacaatgt agaaagagaa caggggatta ttgaacaaga aattgagatg tatcaagatg
     2581 atgcagatta tcgcttgttt acgggaattt tatcatcgct ttatccagaa agtcctttgg
     2641 cctatgatat agcagggaca gttgaatcaa ttgctgcaat tactgcggat gatttacatg
     2701 agaattttga agtgttttat catccaagta acatgaattt attcgttatt ggtaattttg
     2761 atttagaggc agtttggaaa caaatttcaa gttatcaagt ggctcagatg gataatccag
     2821 ctcagtcgtt tgaattggca ggtgtccaaa agttgccgat acaggaacat ctctcagaac
     2881 aatttgaagt atctacccca aaattggctg ttggtttacg gggaaatgac gaggttgata
     2941 aggagactat tcagaaatat cggcttagtt tacaattttt atttgccatg ttgtttggtt
     3001 ggacttcaaa acgatatcaa cagttatacg agcagggaaa aattgattct tctttccagt
     3061 tccaattaga ggttacacct gaataccact atttgattat ctcgggcgat actcaagaac
     3121 ccattacgct ttctagtata ctgatgaaag cacttaggaa attcgaggat gatgcagatg
     3181 tgacagagga tcatttacaa ttgttgaaaa acgagatgta tggagatttt attcgaagct
     3241 taaattctct tgagtttaca gctagtcact ttgtttctca gtattcagaa tatgaaaatg
     3301 tttttgatat acctcaaatg atacaaagtc ttcagttaga tgacatcaga gaggcaggtc
     3361 gtcgctttat tgatcactgt gatatgacag atttcacgat ttttccaaaa taagtgggcg
     3421 caacccgtcg aaatttcttt ggaattttgt tagaatagta tactgtacat gatgagcgat
     3481 agagagagga tattatgaga caaaagagta taggggaagt acttagaaca gctagagaag
     3541 gacggagttg gacatttgtt gacttgcaac ggataacaaa aatccaagct aaatatttgc
     3601 aggcacttga gtacaatgat tttgatttta ttgcaaactc agatgatgtt cagtctattt
     3661 taaaagcata tgctgaggct ttggaattag atgcagatgt tctactggat gcttacgaga
     3721 ccaatagttt agtgaaatat tatgaagatg gagaagaaga actacgctct tcagaattga
     3781 aaagaagcta taaggttcgt aaacgtaaaa agaactctta ccttccgttg atttacttat
     3841 tacttgcgac gggtttgatt attatttttg tgacttatat tgttcatagc aggctccaaa
     3901 atagagcatc tattacgcct actacaacgt catatagtat agttgagcaa tctgtatctg
     3961 aagcaagtag tggcacagag acgagttcta ctgagcaggc gcagtcaaat tcaacgagtt
     4021 caagtagttc cgttacagaa aatgtgacta tttcaggatc aggttctaac attgttgcga
     4081 cccttaaaac agtgacttat ccagtagaag taactgtaac agcaaaaaat acaacgagtt
     4141 ggatcagcct ttctgacacc ttgttagagg ggggcgttac actaacacct gataatccaa
     4201 cagtaactac tactgttgaa gaaggagtga cttctactac acttattctt ggtgttgtca
     4261 aaggagtaga ggtaactgta ggtggccaaa agctaaatct atcagctctg acagcagata
     4321 caggctcaat tacaataaat tttgaacaat aaaggtttta tatgaaaaaa gaaaatattc
     4381 ccaatgcatt gacgataggt aggattttgg taatcccaat ttttatcttg ttactgaccg
     4441 tttgggattc aacaattagt catgtcttag cagcacttat ttttgcatta gctagtatta
     4501 cggattatct agacggctac ttggctcgta aatggcaggt tgtaacaaac tttggaaaat
     4561 ttgcggatcc gatggcagat aagattttgg tgatgacagc atttatcatg cttgtagagc
     4621 ttggctttgc tccagcttgg gtagttgcaa ttattatttg tcgtgaattg gcagtgacag
     4681 gtcttcgtct actcttggtg gaaaatggag gaacagttct agctgcggct atgccaggaa
     4741 aaatcaaaac attttcacag atgtttgcag ttattttcct gctcttacac tggaacgccg
     4801 tggggcaaat aacactctat attgcacttt tctttaccct atattcaggt tatgattatt
     4861 tcaagggtgc gtccttcttg tttaaagata catttaaatg atgaatatta ttgaagtaaa
     4921 aaatttaaaa tataaataca accaagagga tgagcgctat actctaaatg atgtttcgtt
     4981 tcacgtgaaa cagggagagt ggctgtctat tattggtcat aatggttcgg gtaaatcaac
     5041 aacagttcgt ttgattgatg gtttgttgga agctgaatca ggtgatattt atattgacgg
     5101 tgatgcctta accattgata atgtatggga taagcgacgc ttgattggta tggtttttca
     5161 aaatccagat aaccaatttg taggcgctac agttgaagat gatgttgcat ttggtttaga
     5221 gaatcaggga atccctttgg aggagatgcg tagtcgagtt gacgaagctt tggaattagt
     5281 tggaatgact gatttcaaaa cacgtgagcc tgctcgttta tctggtggtc agaagcagag
     5341 ggtggcgatt gctggagtgg ttgccttgcg tccaaaaatt attattttgg atgaggctac
     5401 gtctatgttg gatccagaag gtcgtctcga cttgattaag attgtccgtg aaattaagga
     5461 gcgtcatggt atgacggtta tttctataac acatgatttg gatgaggttg cattgagtga
     5521 tcgagttatc gtgatgaaaa atggacaggt tgaatcaatt agcactccaa atgaactatt
     5581 tatgcgagaa gatttggtag atttagactt ggatagaccg tttacgacag aactggcttc
     5641 atctttgcgc cagactggac ttgatttacc gctccgctat tttacagagg aagaattaga
     5701 ggaaacttta tgggaattga tctccaagaa gtaagctata cctatcaagc tggcactcct
     5761 tttgaggggc gtgcgctttt tggtgtggat ttggagattg ttgatggctc ttacacggcc
     5821 cttattgggc atacgggttc gggcaaatca acgattttac aactcttgaa tggattaaat
     5881 gtaccaacag aaggaagagt agtcattgat gatgttgtga ttacagcgac gtctgaaaat
     5941 aaggatatta agcaagtcag gaagaaagtg gggctggtgt tccaatttcc tgaaagtcaa
     6001 gtgtttgatg aaacggtctt aaaagacgtt gcttttggtc cacaaaattt cggagtttct
     6061 caggaagagg ctgaaacgat tgcgcgtgaa aagttggcct tggttggaat tgatgaaagc
     6121 ttgtttgagc gtagtccttt tgaactatct ggtggtcaaa tgcgtcgggt agctattgct
     6181 gggattctgg caatggaacc aagtatctta gttttggatg aaccaacggc tggattggat
     6241 cctgcgggtc gtcaggaatt gatggatatt ttcaaaaagc ttcatgaatc aggcatgaca
     6301 atcgttttgg taactcattt gatggacgat gtggctaact atgctgattt tgtttacatt
     6361 atggaaaagg gaaagctggt ccgttctggt aaaccagtcg atatttttca agaggtagat
     6421 tttttagaga gtattcagtt gggagtgcct aagattacta agtttgcagc taatttggag
     6481 agaagaggtt ttgtacttga acgtcttcct attacaattg aagaatttac ggggatggtg
     6541 acacatggat aaattaattt taggtcgcta tattcctggg gattcaatta ttcatcgttt
     6601 ggatccaaga agtaagcttt tagcaatgtt tggatttcta ttgatgattt tttgggccaa
     6661 taatttggtc accaatgcct tgttgatagc atttgtttta ggaatggttg ttttatctcg
     6721 tattcgctta tcatttttta ttaatggttt gaaaccgatg ataggaatta ttctatttac
     6781 aactttcttc caggttttct ttacacctgg agcgaccatt ctatgggaat tttggatttt
     6841 taaattatct gtagaagggt tgcaacaggc ggcgattatt tttgttcgct ttgttttgat
     6901 tattttcttc tcaaccctat tgaccttgac gacgacaccg ctcagtttgg ctgatggtat
     6961 tgagtcgggg cttgcacctt tgaagaaatt taaagttcct gtgcatgaaa ttggtttgat
     7021 gttgtccatg agcttgcgtt ttgtaccgac cttgatggat gataccactc ggattatgaa
     7081 tgctcagaga gctcgtggag tggactttaa tgagggaaat cttattcaga aggtaaagtc
     7141 agttattcct attttgattc cgttattcgc atcaagtttt aagagggcag atgctcttgc
     7201 aacggcgatg gaggcgcgtg ggtatcaagg cggagatggg agaaccaagt accggatttt
     7261 ggaatggaag ttagcagata cgttagcaat tttagttatg ttagctttag ctggactctt
     7321 atttatattg aaaaaataag taaaaaacgc tagaaatagc gtttttattg tctaatctca
     7381 atttgtctgt aatatttgta acaatttcac aaagtctaag taatagttag agggtatgat
     7441 agacacatga cagaaaggaa tgtgactatt atgaacatta cattaaagaa aaacttaaaa
     7501 acaactattg ctggtttggt agcgggaatc tcccttttaa ctgcgggtgt agtcagtgca
     7561 gaaacctata ctgtaaagtc tggtgatacc ctgtctgaaa ttgctgaaac atacaatact
     7621 actgttgaaa aattagcaga gcagaataag attaccaact tagattttat ccatgtcggt
     7681 caagttatcg aattgggaga tgtgacagtg aaggcagtag agagttcaat tgaacaaact
     7741 caatcaacat caacttctac tgcaacagta acaactagca cctattcttc aaatttgagt
     7801 gctgaagatg cagcagcaaa agaatggatt gcaatgaagg aatcaagcgg tagctatgat
     7861 gcccgtaacg gtatctacat tggtcgttac cagttgacaa atacttactt gaatggtgac
     7921 tactcaccag aaaaccaaga gcgtgtagca gatgcttatg ttgctagtcg ctatggttca
     7981 tggtctgcag ccaaggcttt ctggttggca aatggttggt actaagataa attagaagag
     8041 gtctagcacg atgctagact ttttctgtct cacgaatgta tttcgcaact actttttcgt
     8101 tcagtccaac ggtactgaat agtaacctct agcccaaaac ttttgatttc cacgctgtat
     8161 ggacataggt gatggaaaat atcaattaag tctagtctaa ttttttagta aatggatttt
     8221 ctgcggtact taggagtgac atacatttgg tatgtgataa actgtaacgg ttttaaccat
     8281 gctaagctag tccactggac tagtttactc ctcctttatt taacttgaca aattagatta
     8341 taacaggaaa aaaatagaag ctcaaagcct tgccagccac cagtataggt ggtatttttc
     8401 ttgtttttct ctatgagaaa actagcccga agccataata agaaaaagga ccgttagggt
     8461 cctttttggt ttacattatt gtcaatcttc cccaaaaatt tcttgagtaa ggcgacgacc
     8521 cgtcggtgta gctgcaaggc caccttcagc agtttcacgg aaggcagttg ggagggcggc
     8581 tccaacttgg tacatggcat tgataacttc atccacaggg atttttgatt caattcctgc
     8641 cagtgccata tcagcagcca ccatagcgta gctagctccc atggcgtttc gtttgacaca
     8701 tgggacttct accagaccag cgacgggatc gcaaatcaga cccagcatat tttttatgac
     8761 aaagcagatg gcttggctgg cttggtaggg agttccacct gctgccagtg tcagggctgc
     8821 ggcagacata gcagaggcag atccgacttc tgcctgacag cctccttctg cacctgagat
     8881 ggaggcgttg ttggcgataa ctagtccaaa ggctccagct gtaaatagga agtcaagttg
     8941 ctgttcttct gtcaaattga gcttttcaat agcaactcct agcacagctg gtagacagcc
     9001 tgcagatcct gctgtcggag tggcacagac taagcccatt ttggcattca attcattaac
     9061 ggccatggca tttcttgcgg cggataggac agcggaatct gacagggttt taccagattt
     9121 catataggca tctaacttgg cagcatcgcc tcctgttaaa ccagatacag atttactttc
     9181 agtaagtcca tctacgatgg aggctttcat aactgtcaag tttcttgaca taattgtcaa
     9241 tatttcttcg cggctacgac cggtcaactc aatttcagtc gcgatcatga gttcagctac
     9301 attagcagaa aagttgtctg cttgctgaac taattcttcg atagtataaa acatgtcttt
     9361 ctctttctag ttgaagaagg ttacattgtg caggtgtgga attttttcaa tttctgcaat
     9421 agaattttca cattggcgag catccacttc gataatcata atggcttttt cgccagcttt
     9481 ttcacgtgtc acgttcattt gggcgatatt gatgtcatac ttagaaagga catcggtcac
     9541 tttcgcaatc ataccaggga catcttggtg aacaataata atagttggcg tattcatatt
     9601 gagattgaca gagaagccgt tgagctctgt tacttgaatg tttcccccac cgatggaaac
     9661 gcctgtggct gagatggatt ttttatcgtt ttttatgatg attctggtgg tattgggatg
     9721 tggggtattg ctgtccttgt tgacacgcca gtaaacctta atgcctcgtt ctcttgcaat
     9781 atcaagagag tcaggaatgc ggggatcatc cgtatccatg cccagaatac ctgctaccaa
     9841 ggctacatcc gtaccgtgac cacgataggt cttggcaaag gaattgtaga gttgaaattc
     9901 cacctctgtc ggctcatcgc cgaagatgga agatacaatt ttcccaattc ggaccgcccc
     9961 agctgtgtga ctagaagatg gaccgatcat aacaggtcca ataatatcaa agaccgactg
    10021 aaattttaag tttgtcatag taatcctcac gtttttcttg gtactattat atcaaatttt
    10081 ctagttagag aatatgccta gatgaatgtg ttttcttatg ttaaaattct atcgcttacc
    10141 ctttttgtat ggtataatac aagttgttca aaatcgtcaa tcgtttttgg ataatcaaat
    10201 tttggaatac gaccttcaaa tcgttatttc tacgaaaaga tgggagaaga ttatgtcaat
    10261 tgacaattct aaaacccgtg ttgttgtcgg tatgagtggc ggtgtggatt catcggttac
    10321 ggctctcttg ctcaaagagc agggctacga tgtgatcggc atcttcatga aaaactggga
    10381 cgacacggat gaaaatggct tctgtacagc aacagaagat tacaaggatg tggctgctgt
    10441 tgcagaccag atcggcattc cttattactc tgtcaacttt gaaaaagagt attgggatcg
    10501 cgtatttgag tactttttag cagagtatcg ggcaggtcgc acacccaatc cagatgtcat
    10561 gtgtaacaag gaaatcaagt tcaaggcttt cttggattac gctatgaacc tgggtgcgga
    10621 ctatgtggcg acagggcact acgctcaggt gtcacgcgac gaggacggca ccgttcatat
    10681 gctgcgtggg gcagataatg gtaaggacca gacctacttc ctcagccaac tctcacagga
    10741 acagctgcag aaaaccatgt ttccgctcgg ccatttacaa aagcctcagg tgagggaaat
    10801 agcagagcga gcaggcttgg cgacagctaa aaagaaagac tcgacaggca tctgctttat
    10861 cggtgaaaag aacttcaaag aatttctagg gcagtatttg ccagcccagc ccggtcggat
    10921 gatgaccgtt gatggtcgtg atatgggtga acacacaggc cttatgtact ataccattgg
    10981 ccagcggggc ggacttggta tcggaggaca aatcggtggg gacaatgagc cttggtttgt
    11041 tgtcggaaaa gacctgtcaa aaaatatcct ctatgtcggt cagggcttct atcatgagtc
    11101 cttgatgtca acttctttac aggctagtca agtccacttt acacgtgata tgcctgaaga
    11161 attcaagctg gaatgcacag ctaagttccg ctaccgtcaa ccagacagtc aagtaactgt
    11221 caaggtgaaa ggtgacaagg cagaagtcat ttttgcagag ccacaacgag caatcacacc
    11281 aggacaagcc gttgttttct acgacggaca agagtgtctg ggcggtggta tgattgatat
    11341 ggcctataaa gatggagagg cttgtcagta tatttaatca aaaataaaag cttagcaccc
    11401 ttgtgctgag ctttttatct tatccccaga aagcatcttc ggcggtttgg tcggctgaga
    11461 agagccaaac ttccttgatt ttgccatctt ccatacggaa aagatcgatg ccattcatgt
    11521 ttagttcttc accgtctgtt cgagtaccga ggaatgttac gttagcggca acaagagtat
    11581 cattatcgga tacccagttt gtcactactt tgaatgttcc atttgttttt tcggcgaaag
    11641 ttgctagatg tgctccgagt acttccttac caattttagc accagatgtt gaatgatttc
    11701 cgggttgatg ccagataatg tcgtctgcca ttgtttcaaa aagagctgca aaatcaccag
    11761 cgatgagagc attgttatag gcattaaaaa tttccaagtt tgttgacatt tgatgtcctc
    11821 caattttttt gatatagtac aattatagtg gaagtaatca attatacaag taggtacttt
    11881 tgcatttgtt ggtatcaaaa gtgatactat tgtgataaat aaaggagaat ttttttgaaa
    11941 aaagttagtg aatatggtat ttgccctttt gcaacaacac aaaaggtctt gactggtaag
    12001 tggggattgg tgattatcta ccaactcagt acaggaacga aacgcttcaa tgaattacag
    12061 cgactgattc caggtatcac tcaaaccatg ttaacaaggc accttagaca attggaggag
    12121 gatcgcatta ttagtcgcac ggtctatgca gaagtccccc ctcgtgtaga gtatagcttg
    12181 actgaaatgg gcgaaaaatt tcgttcggtg ctggatgctg ttgaggcttg gggcttagaa
    12241 tatattgaaa cgttaacgta acaccagatt tgaaattcta caaatctttt gctaaaataa
    12301 gagtaacaat catcatgatg gtcaaagctc atgggaattg taggcttttt tgtcctgcaa
    12361 ttttcgagag ttttggctat tttttgctat tggggagaga agatggattt cagaaccaaa
    12421 gttgacaacc agattttcgg tgtccgtgcc actgcattgc ttataaaaga cgggaaaata
    12481 tttctcacta aagactctaa ggggcgttat tataccattg gcggtgcggt tgaggtcaat
    12541 gaggtagcag cagatgcggt tgttcgagaa gtcaaagaag aattagggat tgacagtcat
    12601 gtcaaccaac tggcttttgt tgtcgaaaat aagtttacac aggaaggcat acactttcac
    12661 aacatcgagt ttcattttat cgtggagcct attggagaaa tacctgagga gatgattgaa
    12721 gataacttga aacaagcttg tgaatggata gacgttgaca aacttgtcaa cctagatgtc
    12781 gtaccagttt ttctggcaga gaaattgcca aactggagtg gacaagttac acacattatg
    12841 aacatgaagg agaaaacaac atgacacaca catttgcaga aaattatgat gtcatcgtga
    12901 ttggtgcggg gcatgcgggt gtagaagcag gattggcagc cagtcggatg ggctgtaaga
    12961 ccttgcttgc aactattaac ttggatatgg tggcctttat gccttgtaat ccctccattg
    13021 gtggctctgc caaggggatt gtggtacgag aaattgatgc cttgggtggc gaaatgggtc
    13081 gcaacattga caagacctat atccagatga aaatgcttaa tatgggtaaa gggccagctg
    13141 ttcgtgcctt gcgggctcag gcagataagg cagagtacgc agcagagatg aagcggacgg
    13201 tggagcgtca ggaaaatctg accctgcgtc aaaccatgat tgatgagatt ttagtggaag
    13261 acggcaaggt tatcggtgtc cgcacggcta ccaaccagaa attttcagcc aaggcggttg
    13321 tggtgacgac gggtacagcc ttgcgtggtg agattatcat cggtgacctc aagtattcat
    13381 caggacctaa caatagtctg gcttctatca cgctggcgga taatctgaaa gaattgggac
    13441 ttgaaatcgg tcgtttcaag acaggaacac caccgcgtgt caatgctcgt accattaact
    13501 acgaagaaac cgagattcag ccagacgatg aaaagccaaa tcacttttca ttcttgtcca
    13561 aggacgagga ttatttgcag gatcagattc cttgctggtt gacctatact aacgcgacca
    13621 gccatgagat tatcaacagc aatcttcacc gagcacccat gttttcgggc attgtcaagg
    13681 ggattggtcc ccgttattgc ccttctattg aggacaagat tgtccgtttt gccgacaagg
    13741 aacgccacca gcttttccta gagccagaag gtcgcaatac agacgaaatc tatgtccaag
    13801 ggctgtcaac cagtctgcca gaagatgtgc agcaggactt gatccactcc attaaaggtt
    13861 tggaaaatgc ccaaatgatg cggacaggct atgccattga gtacgatatg gttatgcctc
    13921 atcagttgcg ggcaacgctt gaaaccaaga agatttcagg gctctttaca gcaggtcaga
    13981 ccaacggaac atcaggttat gaagaagcag caggtcaggg aattgtcgct ggtatcaatg
    14041 cagccctcaa ggtgcagggc aagcctgagc tgattttgaa acgaagcgac ggttatatcg
    14101 gcgttatgat tgatgacctg gtaaccaagg gaacggtgga gccttaccgc ctcttgacca
    14161 gtcgggcaga ataccgcttg attctgcgtc atgacaatgc tgatatgcga ctgacggaga
    14221 ttggtcgtca ggttggcttg gtagatgatg aacgctggca ggtcttccaa atccataaaa
    14281 accagtttga caatgaaatg aagcgcttgg agtctatcaa actcaaacca atcaaggaaa
    14341 ccaatgaaaa ggttgttgcc atgggcttca agcctttgac agacgctctg actgccaagg
    14401 aattcatgcg tcgtccagat gtgacttacg cagatgcggt agcctttatc ggtcctgcgg
    14461 cagaggattt ggacgccaag accattgaat tgattgaaac agaggtcaag tacgagggct
    14521 acattgccaa ggccttggat caggtggaga agatgaaacg tatggaagaa aaacgcattc
    14581 cagccgacat tgactgggat gatattgact ccattgcgac cgaggctcgc cagaaattca
    14641 agctgatttc accagaaacc attggtcagg ctagtcggat ttccggtgtc aatccagcgg
    14701 atatatccat tctcatggtt tacttggaag gacgtagtcg ttccatttct aaaaacaaat
    14761 caaaggacag tcactaaggc tgttcttttc gctgtatgtc agctatatgt caactacttt
    14821 aaaatcatgt gaaaagtagc ttttttactg aatttatggt agaatggcag gcagaggtta
    14881 gtgatgaaaa gatttcgatt ttcgacaatt cactttgtca tgataggtgt cattttattt
    14941 gggttggtgg ctcttgtcca ccgatttttt ccaattggtg ccagtacagt ttttgcgcta
    15001 ctcatcagcc tattggtgtt gattgcgctg tttatttatc aaaaacattc ttatgaattt
    15061 agtgagctag aacagattga atatctcaat aatcaggcaa attctggttt gatgatgctc
    15121 ttggataaaa tgccagtggg agttatcaaa gttcgaccag atagtaatca ggtagaatgg
    15181 tttaatccct ttgctgagct gatttttgct caagaaaatg gtgagtttga tcaaaaaaaa
    15241 ctacgtgaaa ttattgacgt tggtctggat gaggagcgaa tttatgcaaa cttttcaggc
    15301 aagagatacg ctgttaacgt tgattttgat caagggttat tttacttttt tgatgcgtcg
    15361 acagagtatt cagcgacaaa tagtttgatc ggaagccgtc ctgttatagg gattatttca
    15421 gttgataact atgatgagct tgaggatgct gtaactgatt ctcaaattag ccaagtcaat
    15481 tccttcttgg ctcaatttgt ttcggaattt tgccatgatt acggtatcta ctaccgacgc
    15541 ggcacaatgg atcgattcta tttctttaca gattatgctg tgttggaaaa attgatagag
    15601 aataagtttt cagttattga taagttccgc gaggaggcta aaaagttaga tttaggtttg
    15661 actttgagta tgggattggc gtatggtaat gaaaatcatc accaaatcgg acaggttgcg
    15721 ctccaaaatc tcaatatggc tgaggttcgt ggtggagacc aggttgtggt caaggaaaat
    15781 gatgagagta aacagccact tttctttggc ggtggctcgg cttcttccgt taaacgaaca
    15841 cgtacgcgga ctcgtgctat gatgactgct gtatcagata aactcaaatc ggttgatgct
    15901 gtttttgtgg tgggacatcg taatttagat atggacgcgc ttggatccgc ggtcggcatg
    15961 cagtactttg ctcaaaatat tatgaacaat gcctatacgg tgtataatcc agatgaaatg
    16021 gcaccagata ttgcacgtgc catcaagaag ttgagtgatg aaaattgttc taatttgcta
    16081 acggttgagg aagccatgaa gatggtcact ttccagtcgt tgttaatcat ggtagatcat
    16141 tcaaagattg gcttgacttt ggataaggat ttctatgacc aatttaccca ggttgtggtg
    16201 gttgatcatc atcgtcgcga tacagatttc ccaaataatg cagttttgac ttatatagag
    16261 agtggtgcta gttctgcgag tgagttagta acggaattaa ttcagttcca gaatgacaaa
    16321 caccataagc ttaatcgcat tcaatctagt ttattgatgg ctgggattat gcttgataca
    16381 aaaaatttca cctctcgtgt gactagtcgt acttttgatg tggctagtta cttgcggact
    16441 cgtggtagcg atagcttgga aatccagcag attgctgcga cagattttga ggaatatcgt
    16501 caaatcaatg aactgatttt aagcgggcag agaattgagg ccaatgttgt tattgcgtgt
    16561 gcagatgata ctaaagaata cgataatatt attccgagta aggctgccaa tacaatattg
    16621 gacatggcag gaattgaggc ggtattcgtt gtaacaagaa ataccaaagg gtatgtatct
    16681 atttctgcaa ggagtcatag taaaatcaat gttcaacgca tcatggaaga aatgggtgga
    16741 ggtggccact ttaacctagc tgccgctcag gtatatgacc agacaatcgc agaagtatgt
    16801 gaaaagttga cggataaaat ccgtgaagaa ataaatggaa attaggagaa cagatatgaa
    16861 agtaatcttt ttagcagatg ttaaaggtca aggtaaaaag ggtgagatta aggaagttcc
    16921 aacaggttac gcccaaaatt tcttgatcaa gaaaaatttg gccaaggaag caacaaacca
    16981 agcaatcagc gcccttcgtg gtcaggaaaa atctaaggaa aaggctcatg cagagatgat
    17041 tgcagaagca gaggctatca aggctaagtt ggctgaggaa gctactttag tgaaatttgt
    17101 cgaaaaagtt ggtccagatg gccgtacctt tggttctatt accagcaaga aaattgctga
    17161 agaattggac aagcaatttg gcatcaagat tgataaacgc catattcagc tggatcaccc
    17221 aattcgtgcg attggtctca ttgatgtacc agttaaaatc tatcaagatg taataggcat
    17281 cattaatttg agtattaagg aagcttaatt atttatctag ccagtgagga ggtgagatct
    17341 tggcagagtt ggatgaattg cgggtgcaac cgcaggatat tctagctgaa caatcggtat
    17401 taggagctat tttccttgat gaaggcaagc tggtcttcgt tcgtgaatac atcgaagctg
    17461 aagatttttt caaacatgct cataagctaa tttttaaagc tatgattgct ctttctgata
    17521 gaaatgaggc aattgatgcg actaccatgc gtaactattt agtgaaccat ggtgatttgg
    17581 aaaatatcgg tggtttggcc tatttggctg aaatcattag ttccgtgccg acggcagcca
    17641 atgcggaatt ctatgccaag attgttgctg aaaagtctaa tctccgtaaa ctgattgccc
    17701 gtttgacgga tgcagttaat caggcttatg agggtgcgga tggttcagat gacattattg
    17761 ccaatgctga aaaagctttg attgatgtca gtgagggagc cagtcgaagt ggtttcaagc
    17821 ggattgatga tgtcttgaat atcaactttg acaaccttga aactcgtgct aaacagacgt
    17881 cggatattac agggattgca actggctatc ctgctcttga tgctatgaca acgggtctgc
    17941 atgaggaaga gttgattatt cttgcggctc gtcctgcggt tggtaagacg gcttttgccc
    18001 tcaatattgc tcaaaatatc ggtacaaaac tgggtttgac agttgccatc ttttctttgg
    18061 agatgggtgc agaaagcttg gttgaccgta tgctcgcttc agagggagtt atcaattcga
    18121 gggctattcg tacggggcat ttgacgcagg aagaatggaa taagtacatg gtggctcaag
    18181 ctaacctggc tcgggctagt atctatattg atgatacacc aggtatcaag attacagaga
    18241 ttcgttcgcg ttcacggaag ttggctcagg aaacgggcaa tctgggtttg atcttgattg
    18301 actatcttca attaattacg ggaacaggtc gggaaaaccg acaacaagag gtttctgaga
    18361 tttcccgtca gttgaagatt ttggccaagg aattgaaagt tcctgttatc gccctcagtc
    18421 agttgtcgcg tggtgtggaa cagcgtcagg ataagcgacc agttttgtct gatattcgtg
    18481 aatctggatc tatcgagcag gatgcggata tcgttgcctt cctgtatcgt gatgattatt
    18541 atgagcgcgg tgagcaggaa gacggtggta ttcccaataa tacggttgaa gttatcatcg
    18601 agaaaaaccg ttccggtgcg cgtggtaccg tggaactgat gttccaaaaa gaatacaata
    18661 aatttgcaag tatttccaag agggaggatg gatagagaat gagtgatgca tttacagatg
    18721 ttgctaaaat gaagcaaatc aaacaagata ttaaagacca tgagggacag attgtcgagt
    18781 tgacccttga aaatggtcgt aagcgtgaga agaataagca ggggcgtatt gtagaggttt
    18841 atcagtctct ctttatcgtt gaatttgaag atccaaacaa tacctttgtg gaatcttata
    18901 cttactcaga cattttgaca gaaaagattt tgattcatta tttggattaa tcgctatata
    18961 aggctttaca gagccttttt tttgtgctat actgatattt gtgtgaaata acagcagggc
    19021 aaaatgaagc tcgtcaacag aaggtcagca agaagagtaa tcgttgctgt ttcgatgaag
    19081 gcctcctgca cgaatcaggt ttgcttaaaa cgttatttcc tacaaataat ttttatattt
    19141 tattttagga ggacataact atgtcacgtt atacaggacc atcttggaaa caagctcgtc
    19201 gtcttggctt gtcattgaca ggtacaggta aagaattagc acgtcgtaac tacgtaccag
    19261 gtcagcacgg accaaacaac cgttctaaat tgtcagaata cggtttgcaa ttggctgaga
    19321 aacaaaaatt gcgtttctct tacggtttgg gtgaaaaaca attccgtaac ttgttcgtac
    19381 aagctactaa aatcaaacaa ggtactcttg gtttcaactt catgcttctt ttggagcgtc
    19441 gtttggacaa cgttgtttac cgtcttggtt tggcaactac tcgtcgtcaa gcacgtcaat
    19501 tcgtaaacca cggtcatatc cttgttgacg gcaaacgcgt tgacatccct tcataccgcg
    19561 ttgaagttgg tcaagtgatc tcagttcgtg agaagtcaat caaagttcca gctatccttg
    19621 aagctgttga agcaactctt ggccgtccag ctttcgtatc attcgatgct gaaaagcttg
    19681 aaggttcatt gactcgcttg ccagaacgcg acgaaatcaa cccagaaatc aacgaagctc
    19741 ttgtcgtaga attctacaac aaaatgctct aagatttgta tcgataattt aaaagcccta
    19801 tttacaaggg ctttttgtct gtatttgagg aggggattat gtggcgtaag tattttttac
    19861 tagtgctatt agctattttg gtcgtaggtg gggccttctt tctatctcaa tcagcggcta
    19921 cgccaaatga atcagactat gtgaaggctg taaagagtta ccaggctatt tcgatagatg
    19981 aagtggagca aaaggtccaa gacgaggaag aatttatatt atacataggt cgtgagacat
    20041 gcccttattg tcgtgatttt gttccgaaat taacagaggc agtagagcga tcacgtgcga
    20101 ctatctacta tttggatagc gagtcggatc cgaatggaaa aatacaacaa tttcgacaga
    20161 gtcaaggtct tgctacggtt cctagcctaa cctattataa atcgggtaaa ttatctggaa
    20221 tacttcgtaa agggagtcaa gcaacgctat ctgaaataga gaatttttta gctcttcaag
    20281 ggcagtaaat agttttaaga atgtatgaag cgaatatcat acattttttt gttcatgttt
    20341 gtgaaaatat ttcaaaacag attgaaaaac atttcacaat cttgtataat actaatatca
    20401 gataaaaatt ggaggtttat atgaaaaaga aagcttggtt aggcttatta atgagtctac
    20461 ttgctgtttt gtttttagtg gcgtgtggag gtaagtctga aaagacagct acatcttcat
    20521 catctacgac ttcttcttca tcagaagaag ctgtttcagg tgcatctgag aaagtttata
    20581 cagatccatc tgagttgaaa gatagctacg atgttatcgt tgttggttca ggtggtgcag
    20641 gtatgtcagc agctatctca gctaaagacg ctggtgctag tgttgctctc ttggagaaaa
    20701 tgcctgttat tggtggtaat acggctaaat catcagctgg tatgaatgct tcacagacta
    20761 aattccaaga agcggaaggt attgctgata caaacgataa attctacgaa gaaacgctta
    20821 aaggtggtaa agggacaaac gatcctgaat tgcttcgtta tcttgtagat aactctgcta
    20881 gtgcaattga ctggttggat ggaatgggga tcactcttag caacctaact acaactggtg
    20941 gtatgagcga gaaacgtacc caccgtccag cagatggttc agctgttggt ggctacttgg
    21001 taaatggtct ttaccacaat cttgttgaac gtaaagtacc aatttttgtc aatgcagatg
    21061 ttacaaaact ccaagacaag gatggagcag tgacaggtgt tgaagtgaag attgctggtg
    21121 aaacgaagca aatctcagct aaagcagttg tattggcgac aggtggtttc ggtgctgatt
    21181 tggaaatggt tgctaaatta aacccagcat tgaaaggtta tgtaacaacc aaccaagaag
    21241 gttcaacagg tgacggtatt gcacttgctg aatcagaagg tgcagcaact gttgatatgg
    21301 aacaaatcca aattcaccca agtgttgagc aagaaacttc ttacttgatt acagaagcag
    21361 ttcgtggcga aggtgctatc cttgtcaact caaaaggtga gcgttttgtt aacgaattgg
    21421 aaactcgtga taaagtttcg gcggctatca atagtttgga tccaaactat gcttatgtta
    21481 tctttgatga tgccttgaaa gatcgtgtga aggctattgc tcaatacgaa gaaaaaggtc
    21541 ttgtaaaaac gggtgctaca cttgcagatt tggctaaaga aattgaccta cctgcggaca
    21601 cacttcaaac aactcttgat acttggaaca aggctgtggc agataagaat gatgcggcat
    21661 ttggtcgtac atcaggtatg gaccatgctt tgaacactgg ttcttaccat gccattaaag
    21721 ttgcacctgg tgtccaccat acaatgggtg gtgtgaaaat caataccaat actgaagtat
    21781 tgaaaacaga cggttcagca atttctggtc tttatgctgc tggtgaagta actggtggtg
    21841 ttcatggtca aaaccgtatt ggtggtaatg cagtagcaga catcatcgta tttggtcgtc
    21901 aggctggtga acaagcagct ggatttgcta agaaataaat tgtagacata agaggctcgg
    21961 cagaaagaag gtcgagcttc tttttcttgt ccctctatct ctaagatgat atacttttaa
    22021 ggtatgaaga agaaaatagt attattgtta ggagctattg cagttatagt tttgacttgg
    22081 tctgctattg ctggtcagcg tcagcaatct tatgccgagg ctgaattaag ggaccaggat
    22141 aaaatctgga ttatcactga tttgcattat ttgtcacagg atttatttga tgatggagag
    22201 gcattttctt atatcgaaaa gacagctgct ggcaaggatt tacgttatgg aaaagagcgg
    22261 atggaggctc tggttgagca ggttgggcgt gaacatcctt ccttgttact agtcagtgga
    22321 gatttgactc ttaatggaga aaagcagagc atggttgaat tagctcagta ttttacagag
    22381 attgaagaac aaggaactga ggttttggtc attcctggca accatgatat tgccagtgga
    22441 tgggctaggg cctttaaggg agaccagcag atagtgacgg atcaggtcac cgctcagcaa
    22501 tttgcagagc tatttgccca tcacggttat caacaagcta gcagtcgtga caaagcctca
    22561 ttgagctacc tggccaaacc attttcaaat gcttggtttc tgatgataga cagcaatatc
    22621 tactctgacg gctatggaaa aggagctcct ccaaccaatg gacgtatcaa taaagagact
    22681 ctagaatgga tagaaattca gcttcaagca gccaaggaaa ctggagtgag cctgatacct
    22741 gttgtccatc acaatgtctt gcaacagcat gccatgcttt caaaaggcta tactttggat
    22801 aatgctgctg atttgaagac cttgttcaat caatatggca ttcaatttgg tttttcaggg
    22861 cacactcatt ctcagaatat tgttaaggaa gacttgggac aagttaacta tactgaagta
    22921 gtcaatggag cattttctat ttatccagct attatcggcc aattgagcct tgatgataca
    22981 agcattcatt atcaaaaaac tcaacttgat atggcttcgt gggcagataa gcaccaacct
    23041 agtgatccaa atttgcagga tcacgttacc tacttacaaa ctgtctttga caataccagt
    23101 gacatcatgg tccacaatat gttaaacgat gaaggttggt atgatggtga aacagcagat
    23161 gctatttcac aatttatcat gccggccaac cgtgcttatt tttcaggaga aaaactagat
    23221 cagacttggc tagatgagaa ggtatttcca agccaggctt atcagacctt gcaagcagca
    23281 tcaccgagaa gtttcttggc tgattatctt gatgtgatta taaaacggag tcaaaatagg
    23341 gatgtagaaa aactagtgct taaaacagaa aaagattttg tccaatagtt ttccacagat
    23401 tgttgaaaaa aaccacaact tatccacaag ctgtgaataa gttatgccgt agtaactatc
    23461 cgagtaggtt tgtttttaca aacaaaaata ctcagcaact ttcctgaaaa tgtgataaaa
    23521 tagtaacaaa ttgtgtcaaa tggagagaaa gatgacctta ctaagcattg ctattccgag
    23581 ttataattcg gaggcttacc ttcattactg cgttcattcc cttgtaatgg gtggggacaa
    23641 ggttgaaatt ctgattatta acgatggttc gtcagaccga actcaggaaa tcgcagagga
    23701 gcttgccaat caattttcaa atgtacgggc tatttatcaa gaaaataaag gtcatggcgg
    23761 agctgtcaat acagggatac gtgaggccaa ggggaagtac tttaaagttg tagattcaga
    23821 tgactgggtc gataccaggg cctatttgaa gatcctagag agtttgcagg ctttggaaga
    23881 tgaaaatacg ccagttgatg cctttatctc caactttgtt tacgaaaaag agggacaatc
    23941 tcgcaaaaaa tccatgtctt atcaatccac tctacctgag aaccgtatct tcggttggga
    24001 agacgtgggg gcattttcca aggggcaata catgatgatg cattccttga tttacaggac
    24061 agatttattg cgtgaggttg gtctcgttct gccagagcat actttttatg tggataatat
    24121 tttcgtattt actcctttgc aggctgtgaa aacgatgttc tatctgccag tagattttta
    24181 tcgttacttt attgggcgaa acgaccagtc tgtcaatgag tccgtcatga ttaagcgcat
    24241 tgaccagcag ttaaaagtca atcgaatctt ggtagataat ttagacttgg aggcgattga
    24301 caatcctgat ttacgaagtt atttgctcaa tcatgtggag attacgacca ttatctcctg
    24361 tgcccttctc aatcgtgcag gaacagctga gcatatgatg aaaaagcagg aattatggca
    24421 ctatattcgg gataacaatc cagctctctt taaaattgtt cgtaaagggt tactcgggca
    24481 attaaccaac ctttacggtt acccaggtcg aaaaattttt aatgccgtct ataaaatcgc
    24541 gaaaagaatt tatggtttta actaaaactc agtttcggct gagtttttct gttataataa
    24601 ggaatatgaa aagcgtttac aaaggagaag ataaatgcaa agattaggac aaacaggtct
    24661 agaagtttca cgaattggtc taggttgtat gcgaatggct agtctgacag agcaggaaac
    24721 tgctaaagtg ttagatacag tcgttggaca gggaatcaac ttttttgatc atgcggatat
    24781 ttatggtggt ggtgaatcag aaatccgttt tgctcagggg gcaaaattgg cgggattgaa
    24841 acgtgaggac ctgcttctcc aatcaaaatg tggcattcgc aagggttatt ttgatttttc
    24901 taaagactac atcttggagt cagtggacgg tattctcaag cgcttggata cggaatattt
    24961 agacgttctc gccttacacc gtccggatgc tctaatggaa gcagaggaag tggcagaagc
    25021 cttttatctc ttgaaaaaag cagggaaggt tcatcatttt ggcgtcagca accaaaacat
    25081 ttaccaaatg gagttattac aatcctattt cgatcagccc ctagtagtta atcaattgca
    25141 gctctctcct gctcacacgc ctttgattga tgctggatta catgtcaata tgaaagatga
    25201 tgctgcgact atgcgtgatg gtggactgat tgattattgc cagcttaaaa aaatcactat
    25261 tcaggcttgg tctcctttct tgattgattt gcaacgaggt atttttgcca atcatccaaa
    25321 ctatgctctt ttaaatcaaa ctattggaga aatagcagag cgatacaatg tttcacatga
    25381 aacaattgtc gtggcttgga ttttgcgtca tccagcaaag attcaaacca ttgttgggtc
    25441 catgaatccg gagcgcttaa aaaagattgc acaagctagt cagattatct tgactagacc
    25501 agaatggtac gacatttata gaagtgcggg caatattttg ccgtaggagg caactatgca
    25561 gtgggaaata aaagcatttg accaacttag tttacaagaa ctttacacta tcttgacact
    25621 tcggaccgat gtgtttgtcg tggaacaagc ctgtccctat ccagaaatag atggaaaaga
    25681 tccaagttgt cttcatcttt taggaacaga caatggggag ttagtggctt atttgagaat
    25741 cttgccagct ggtctaagtt atgatgaagt ttccatcggt cgtgtggtca tcaagccaag
    25801 ccatcgtggt aaaggattgg gtcggccaat gatggagcaa gctatcgctt acattacaac
    25861 cgagtggaag gaaagtcaga ttaaaatcgg ggcccaagcc tatttggaaa aattctatca
    25921 atcgcttggc ttcgaaccta tttctgaagt atatttggag gatgatatcc cacatttgga
    25981 tatgttatac cgtaaactag tcgtttaagg ctggtttttc gatttttctc ggcaatttcc
    26041 cccaaaaatc agccatttta tggtagaatg aataggtata aaatcagtaa aagaggaaga
    26101 aaatgaaacg ttctatgtac gctggacgtg ttcgtaagga acatgtcgga caggaaatta
    26161 ctttgaaagg ttgggtcggc cgccgtcgtg acttgggtgg cctgattttc attgacttac
    26221 gtgaccgcga aggcattatg caattggtga ttaaccctga gtcagttgaa gctgaagtga
    26281 tggcaaaagc ggagagtctt cgttctgaat ttgttatcga agtaacagga acggttgtgg
    26341 agcgtgagca agctaacgac aatatcccga caggagctgt tgaactgcaa gtaaccagct
    26401 tgacagtgtt gaacacggct aagacaactc catttgaaat caaggatggc attgaagcca
    26461 gcgatgacac tcgtcttcgt taccgttatt tggatcttcg ccgtccagaa atgctcaaca
    26521 acttcaaatt acgtgcggct gtaacccaca gcattcgtaa ctacctagac gagcttgaat
    26581 ttatcgatgt ggaaacgcca atgttgacca agtcaacgcc agaaggtgcg cgtgactact
    26641 tggtaccatc tcgtgtgtcc aaaggtcatt tctatgcttt gccacaaagt ccacagatta
    26701 ctaagcagct tttaatgaac gctggttttg accgttacta ccaaatcgtt aagtgtttcc
    26761 gtgatgaaga tttgcgtggt gaccgccaac ctgagtttac acaggttgac ttggaaacct
    26821 cattcttgaa tgaggttgaa atccaagaca tcgtagaagg cttgattgct aaggtcttga
    26881 aagataccaa aggaatcgat gtgaccttgc cattccctcg tatggcctat gaccatgcta
    26941 tgaacttcta tggttcagac aagccggata ctcgttttga gatgcttttg caagacttga
    27001 cagaacttgt caaagaagtt gacttcaagg tcttctcaga agcaccagtt gtcaaagcca
    27061 ttgtggtcaa gggtgcggcg gattgctact cacgtaaaga tattgataaa ttaacagact
    27121 acgcaaaaca atttggtgct aagggacttg cttgggttaa agtagacaag ggagaattag
    27181 cgggtccagt tgctaagttc ttgacgggta ttacagaaaa cttgacagca agtttacaac
    27241 ttgaagacaa ggacttggtt ctctttgtag cagatgaatt ggaagtagcc aataataccc
    27301 tgggagcttt gcgtaaccgc ttggcaaaag agcaaggttt gattgacgaa agtaagttca
    27361 acttcctctg gattgtagac tggccaatgt ttgagtggtc tgaagaggaa ggtcgctata
    27421 tgagtgccca tcacccattc accttgccga cagaagaaac agctcatcat ttggatggtg
    27481 atttggcgca ggtgcgtgca gttgcctacg atatcgtttt gaatggttat gaattgggtg
    27541 gcggtagcct tcgtatcaac caaaaagaca tgcaagaacg gatgtttaaa gcgcttggct
    27601 tctctgctga ggatgcccat gagcagtttg gtttcttgct tgaagctatg gactacggct
    27661 tcccaccaca tggtggattg gctatcggtt tggaccgctt tgtcatgtta ttggctggtg
    27721 aggataacat tcgtgaagta attgcctttc cgaagaacaa caaggcatct gatccgatga
    27781 cacaggcacc gagcacagtt gcatcagcac aattagagga attggcttta aacattacac
    27841 ttgaaaatga ataaattttt cagaaaacag cgtgtacgct ttttaagaaa ggccagaaaa
    27901 aataagtttt ggtcggtcat tttgggaatt tctcgggaaa aatatgccga acggatatcg
    27961 gggtccatta tttatggact cctatctagt attgcagtca attttttctt tagaccaggg
    28021 aatgtttatt cgtctggagt tactggtctt gcacagattg tttctgctct ggcagcttct
    28081 tacgctggtt ggaatgtacc gattgcggtg gtctactacg gcctgaacat tcccttgctt
    28141 atcttagctt ggtataaaat tggttataaa tttactatct ttacctttat tacggtatcc
    28201 ttcagttctt tctttatcca atttatgccg ccagttaccc tgacgacgga tccgttagta
    28261 aatgctattt tcggtggtct tatgctggga actgggattg gctttgcact taggaacaat
    28321 gtatccagtg gtggtactga tattgtctct attattattc gcaagaagac gggacgtcaa
    28381 gttggctcca tttccttgat tgtcaacatt atgattatgt tgattgcagg gatgaccttt
    28441 ggctggcaat atgccctgta ttcaatggtt gcgctgttta tctccagccg catgacagat
    28501 gcgattttcg tgaaacaaaa acggatgcag gccatgatta ttaccaatca tcctgaacga
    28561 gtcattaaga aaatccataa gaagctcaat cgcggtgtga ccattatcga cggtgctgag
    28621 ggtgcttata accatgagca aaagacgatt ctcattacca tcatcactcg tgcagagttc
    28681 aatgatttca agcatattat gaaaaaggca gaccccaaag cctttgtatc ggtagctgac
    28741 aacgtcaata ttattggtcg gtttgtggaa agtgactgat tgacaaacac tacatgtagg
    28801 agtaaaatag tatttacagt ctacatgtag tgtttttatt ataaagagga aatgtatgaa
    28861 gaagaaaata tatttggttt acatcagttt gagtggcaat acagagagtt ttgtgaaacg
    28921 tctaaagtcc tttttccaat ttcaaactga ctgggagatt gagctggtcc atgttaaaga
    28981 tttggtcaag caggatattc ctttctatca gctagatgca ccctttgtcg cctttttacc
    29041 aacctactta gaaggtggaa atggagtgga caacggagat gtggaaattc tgaccaatcc
    29101 tctcggagat tttatcgctt tggggaccaa tgcagagctc tgtctgggtg tcgttggctc
    29161 aggcaaccgt aattttaaca accaatattg tctgactgct aagcaatatg ctgagcgatt
    29221 taattttcct gttatagaca cctttgaact cagagggatg cagaatgaca ttgaacgcat
    29281 tggtcggaag ataatggaat tgatgtaaag agcctcaact cagctttatg ctgggttgtt
    29341 tttttggtca cacaaaaaaa actcaccagc gtgaccgatg agttttaagg agattatgaa
    29401 aaatatttag gattgactat agtatacctc agactatatc gtgtttcatc ggtctaaagt
    29461 cctatttgtc ttctaaaatg caaatactct gtcctgcaat cgtgatttct tgctgatcac
    29521 gctcttcgac tggaggatag ttgtagagaa ctgccttatc tttttcacgt tcataggtaa
    29581 agtcgctatc cgcaaagttg atgataaatt gaatggtctt ttcttcactt gtcacttgat
    29641 agcgaagaac atattcagtt aaccagtaga aatcacaagt ttcttggata ctttcgtagt
    29701 tatcctggct gagtattgga ttattttttc ggaaggtaat taattcctga atgaactcga
    29761 tatgttcctt ataacgaata gctcgtgtcc agtccagacg gttgatacgg tcagggacat
    29821 tgtaagtatt atctatttca tcttttgtac ggaaaaattc ctgtccgctg tgaatgaagg
    29881 ccataccctg agagattaag atgagctgga gaccaaaact tgcagcacgt ttctgctgtt
    29941 gaggtgtcca gtttggattc tcaatatgga aatagtcaaa tgctgtagca ttatcatggc
    30001 attcgatgta gttgagggac tgttgaggcg ttaagaaatg gcttaatcgg ctaccagtca
    30061 ggagatgttg taccttttcg tgcaattgct tatctactag acggtttgga ttgagcaaga
    30121 gtttcttaaa ggtatcacgg tagtcgtcat tgaagaagct gatttcaggt aactgctccg
    30181 cattgtactg gtgagccagc ttttcaaatt cgaggcctgt tgccattttc catccttcac
    30241 catagaggta gacatttggg tgaatggcag ataattcaat ttgaatctgg ttgattgttt
    30301 cgctatctaa aatacccata aggtcgaaac ggaagccatc aaaaccatag agttctagcc
    30361 attggcgaag ggattgtttg atgtagttgc ggaccatact gcgctcgctg gcaacgtcgt
    30421 taccgcagaa tgtcccgtct gttcgcatgc cgtggtcatc atagcgatag aagtagcctg
    30481 gaacgatgag ctcaaatgcg tacttttcag catgatagac gtggttgtaa acaacgtcca
    30541 taataactga aatatctgca tcatggtagg cctggatagc ttcttgtagc tctagaatac
    30601 gtgcgtaagg atcgtttggt tggctagaat aggaaccttc tggtagattg tattgcatag
    30661 ggtcgtagcc ccagttgtaa acagcttggg gtttattttc atccacactg ccaaaatcat
    30721 aaacaggcaa taattgaata tgggtaacgc ctagttctct gatgtaatcc attcctagct
    30781 tcattccatc catggtagga gattctgtca atccgagaaa ttgactgcga tgcttgaacc
    30841 cagcttcttt ttgccatgag aaatcacgga cgctcatttc atagataatg gcctgagaaa
    30901 taggtcgttg ggtcttagca cgacgaattt tatgaagttt atctggattg ataacgtagc
    30961 tgtcacctga gttggctgtg gaagacaggg cgtagggatc gtgaacggaa atccattcac
    31021 tgtttacttt gtggagatag tgatagctgt gcgcttctag gtctccttca acagtaattt
    31081 cccaaactcc ctttgctccc ttcgtcattg catgtgggtt tccttctaga acaagaaaaa
    31141 cttgcttaga aataggtgcc cataacttaa aggttgttgc ctctggtcga taggttgctc
    31201 ccaagtcttg cccattgtat gtataacttt gttcaaaaag tgtagagcga acgatatgac
    31261 catagccgag ttctgtttta ttacggtctt ggtcgtagat ggtatagtcc ttgtccaatg
    31321 tcagtgcatg gaggctggtt agataataga tgacgagatt atctacttct agacaggaat
    31381 ggataaaaag ttgagatgta gcatcatttg attcaagtga gaagctcata tgttcggagt
    31441 cgaatcgttt ttccatgacc aagcgaatag ttgctacatc gtcaagagat gcttgaaatt
    31501 gtcgtaatgc catgagccaa aaggctcctt tctaagttag agttcggtaa tggtatgtgg
    31561 aatgacacgt cggtagcaga cttgcttgtc ttccttgttg tcattgatga tttggtgcag
    31621 agtgttgaat gcgacgcgtc ctagtttaat agcgtggacg tcaacgtagg cttcaatatc
    31681 tagttttggc ttgacggagt caaaagaaat gataggaagt ttgactccga cttcgttgag
    31741 atatttgaca acaccttcgg caacggaagt gtcagttgtg acgattgcat ccaggtcttg
    31801 tttgagtaat ttcttagaaa ttttgtaggc actgtcttcg agaaggaagc cagacacaaa
    31861 cttgaccttg ttttcatcca ggggaatatt gtgcgattta agggcgttct tgtaacctgt
    31921 ataacggtct tgagatacga ctaactcttt gttaccagcg acgaaggcaa tgtttttgta
    31981 acctttattg atgaagtagt tggtagcttc aaagccggcc tggatattat cattatcaac
    32041 tagtgatatg aatggagaat ccgccttacc aagaatgagg aaggggaatt tgtgttggat
    32101 agctaattgt accaatggat cgtcgggctt ggaatagagg aagatgaggc cgtccactcg
    32161 tttgccgtag accatctgag aaatcgcttc tagacgttgt tcttcattct ttcctgtgga
    32221 gatttggatg gcataattat gatctgccgc aacttgagaa attcctcgta aggcagtagg
    32281 gaagaagggg ttttgataga aaacatccga gtcatctggc aaaaccagtc cgataacctg
    32341 tgtatagcta gaaactaggc ttcgagcgtt gaggtttgga tggtagttga gatccgccat
    32401 ggctttccta accaagtctt tcgttttttg gctaatggca gagctatttt gaatgacacg
    32461 agtaactgta gatggcgaga caccagctaa tttggccaca tctttgatgg tagcacgcat
    32521 atagacctcc tagatttcat ctctgaattt tgttttgaaa aatacccata acaagcatag
    32581 cacaaatagg atattttgta tggttagaag ggtagtcaca ggttgaccaa atagtccaat
    32641 tagacaggct ataagagtag gcaggcccag acagttgaga gcaaaattat agcactctgc
    32701 aaacgtttca aaatggaaga aacgagactt tttagtcaaa aagaggaaga agctggctcc
    32761 tagtaggatg aagagtagtt ttgttgttaa aataaaggtc gaaataagag tgatggacag
    32821 tccgacagct gctcggttgg cttgatacca ttttttagag atggttgcag tcaaggtctc
    32881 cttactagca aaatcgtcag tattcaaacc ttcataagtc aattcgctta gaatatcact
    32941 tcctttacga atagtcagag actgattggc gaattgataa gtaaagtcag aactagagct
    33001 aacggtatca ccaaatgtca cttgctccca gttagtattt ccagtatagg ttaggtggtt
    33061 gttttcaatg actgcatatt gacggaggtc atctacgacc tgatctgtca gaggtgcata
    33121 gacaccttct acgaaatttt ctaaaggata ggtttcgaga ttgacagtct gtagggagct
    33181 cggaatgagc aagagcgcca ctaaaaaaag cgttgtaaac aaccgttgcc aaaggttcat
    33241 tagtctacga ttggcaaaca tatttcttgg agagaagata ctagaaaaat aggaaaaagg
    33301 atatgggagc attcgttgga cctttcataa atatcaatgt ttcccctaaa aagggaaaag
    33361 gtgggacagg gttatccctt gtcgccaccg cttgttaaac cagatacaaa gttcttttgt
    33421 aggaagaaga ataggatact gatagggagg gcaataagaa tggcacctgc cgcaaagtag
    33481 gcaatcttca tattcttcac atcactgatg aaggtttgaa gtccgacagc tacagtatag
    33541 aattcttttt cacgaagcaa gaatttggat aggatgtaat ctccaaaagg tcccatgaag
    33601 gcccagagcg cttgtacagc aatcattgga cgaacgagtg ggaggacgat ttgccagaaa
    33661 cgtctgaagt gaccggcacc gtctagtttt gcagattcat caagtgagat tggcaccgta
    33721 tcgaagtagc ctttcatgag ccaagcgttc attgggatac caccaccgac ataaaggaag
    33781 atgaggaacc agctttggtt gagcgcattg agcatgaggg ccataacgaa gaaggcggta
    33841 agagcagcca tggttggtac catttggata atcaagaaga agaccaaact ctgcttacga
    33901 gcgatgaagt tatagcgact ataagcgtaa cccgctagta ccactatact tgtttgaata
    33961 accattgtca agagagcaat gataagcgta ttgaagtacc aggttccgta tagagtctcg
    34021 ctaaagagct tgctaaaatt gtctagtgta aagttgatat tgctatccaa tttgaaggca
    34081 accacgttac cagacttgaa ggcggacatg attgtaatca agagaggata gatgatgata
    34141 acagacagaa caatcagata gagataggtt aaggtctgat tgagtatgcg tctagtcttt
    34201 acagatactt tcatcttata catcctccat ttcaaaagca tttagttttt tgaaggcaat
    34261 catggaaata ctgatgacaa tcattgagat aatcaatgta actgctgccg ccattgagta
    34321 ttgaggtgat gtacctgtgg ttaatttata aatccatgaa atcaagatgt cagttgaacc
    34381 cgctccacca ccgacgctac caggaccacc attgttgaag aggtagatga tagagaagtt
    34441 gttgaagttg aaggtgtatt ggctgattag tgttggagct gctactgcta aaatcatcgg
    34501 aagggtgatg ttacggaatt tttgaatggc acttgcacca tcaatgtaag cagcttcata
    34561 caagtcgttt ggaatggatt gcaaaatacc caaggtcaaa acatagatat aagggaaacc
    34621 aagccaagcc tgcatcataa tgagagcaac tttagtccag aatgggtcgg tcttccaagg
    34681 gataaggaaa ttgtcgatga atggtaggaa tttgctcagg aatggcaata cctgtgtgtt
    34741 aatagcgccg acactatcgt tgaacatgtt actaaaagtc aagatagtta cgaaggccgg
    34801 aacagcccat gggagcaaga agataacacc gaagatacgt tttcctttga tgaatggttg
    34861 atttgcgatg atggctgtca agataccgat aacaatttga gctgttgaag cgcagagtgc
    34921 ccagataatt gtccagccaa gtacagaacc aaatgctgta cggaatgtgc ttagtttcca
    34981 gatatttgta aagttggtaa taccaatcca atccaacaat gcgcttggtg gcaggtgttt
    35041 gaagtcgtag tttgtaaagg cgataaacaa tgtaacgaca actgggaaga tgatagcaaa
    35101 ggtcatcgca atatatgatg ggataatcaa gagatatggg aaaccattct catagatacc
    35161 ttttaccatt tcttttaatg tggtagccac tggcatccct gaattccatc gtttcgcggt
    35221 atcacgtgcg tcttttaggt tgagtgcata gaaagcgaga tagacaactg tgataattaa
    35281 gtggaaagaa ccacgaatca gcatgaatag cgaattatct tgtccacgaa cactaccgag
    35341 agtaatcaag ttgcctaatt caccagctgc aatagcaata aaataaacaa ggaaggcaag
    35401 cgtaacacct aaaaagatgt atcctttggc tttttgttta ttgtagattt gtccgagacc
    35461 agggatcagg gacaaaagga gtgccttacc tggattttgt ttcatagtca tagtctcctt
    35521 atatagcttc tgaatagacg gtggatgtta tcgtcattta gtttttcttt tattacttcg
    35581 tta
//
LOCUS       DASFSW010000005        35541 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.005, whole genome shotgun sequence.
ACCESSION   DASFSW010000005 DASFSW010000000
VERSION     DASFSW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35541
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.005"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            27..1937
                     /locus_tag="U1099_000216"
     CDS             27..1937
                     /locus_tag="U1099_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM4866567.1"
                     /translation="MAKDYTDLATDIVAHVGGKENISSLKHCVTRLRFGLKDESKADT
                     DYLKARDGVVTVVQAGGQYQVVIGNHVPDVYAAVQKVAGISGDGALDIDEGDGPKGNL
                     FDRFVDLVSGVFQPSLGVLAAAGIIKGLVSILAATMGLNNDNSALYAILNTAGDGFFQ
                     FLPIVLAFNAARKFRMNEFTGMAIAMALVYPTLPGTFATLKEAGLNNVFGIPFVLPAA
                     GSYLQTVMPVLLAVWIASHIEKFMKKITPDVVKVFIVPFVTILISVPLTFLVVGPVAN
                     YASDLLSAGFTAVMGFSPLLYGILLGALWQVMVMFGLHWALVPLAILQFSQNGWSNIL
                     LAAALPNFTQTGVLLAIMMKTKESKVKSVAMPAFISSIFGVTEPAIYGVTLPMKTPFY
                     ISCAVSAVIGAGLSFFDLKNYAMGALGIFMYPGYVSPELGVTPMWIMMGFSLAAIVLS
                     FAIQMALPVPYLYGEPTEKKSVEETKESVPELKEIKQEIIASPLIGKVVKLEDVPDAV
                     FASGAMGKGIAIDPLDGILVSPAKAEVTLLFPTKHAIGLRTENGAELLIHIGMDTVSL
                     AGKGFESFVQVGDQVEAGQKLLEFDLHQIKAADLPVITPIIVTNTADYEDILVTQESQ
                     INSGDYLLTTVK"
     gene            complement(1982..2311)
                     /locus_tag="U1099_000217"
     CDS             complement(1982..2311)
                     /locus_tag="U1099_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEM4866568.1"
                     /translation="MITMHLYYTGPADNARAFAQEMEASGIADRIRQQAGNTRYQYFA
                     PLDDPHSILLVDSWTDQAALDIHHASPMMQEILDLRSKYQLTVTAERYKSDEAGIPEQ
                     DKDFLQR"
     gene            complement(2561..3475)
                     /locus_tag="U1099_000218"
     CDS             complement(2561..3475)
                     /locus_tag="U1099_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM4866569.1"
                     /translation="MQPPLTDKQFKHTIDYDSRLLPYKYYNTYVTDGIPDILFHWHNE
                     FEINYVYGGTARYHIDYDYFNSQAGDIILIRPNAAHSIHPIEQRGHETDTLLFHLDML
                     GASHLDQTSMLYLQPLQTDVFKCIPRIQPQDPGYAEIRQCLLTIFELVRHQPPYFELP
                     LKSRLFDFFYLLYRYRYIVRKNTDDMYRKNEKLRLLIEYIQQHYAENITIEQLAEQMG
                     YTKTHFMTIFKQQTGTSCMEFIIQVRLRAATELLTNTIKPVLEIATDVGFNNLSNFNR
                     QFKQYYHTTPSSYRKQLRKNRTCTNFDK"
     gene            3586..5067
                     /locus_tag="U1099_000219"
     CDS             3586..5067
                     /locus_tag="U1099_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEM4866570.1"
                     /translation="MSRFPKNFLWGGAIAANQAEGAYNVDGRGLVQTDVTTGGSVNSP
                     RYTTYIDKDGKPGKYASMGHAGGHLPEGATYAVLEDHYYPNHKAVDFYHRYKEDIKLF
                     AEMGYSVFRLSISWARIFPNGDDAEPNQAGLDFYRSVFEECRKYGIEPLVSIWHFDTP
                     LSLEQRYGGWTNRKLIDFYVRYAETIFNEYKGLVKYWITFNEINNTIMFLDMFSEPED
                     SDYQEAYQHLHHQFVASAKAVKLGHQIDPSYRFGNMICGITFYPGTCDPADILENQHK
                     WEQNIFYCSDAQAKGRYGSYANRLWKEHNVVLQMEEGDLEILAEGKVDMYTFSYYMST
                     VVTTHKVEDTVSGNFSIGAKNPYLTYSDWGWAQDPTGLQYYLEKIYDRYEIPMMIVEN
                     GLGALDTVEEDGSIHDDYRIDYHRAHVNAMADAIANGVDLIGYTTWGCIDLVSAGTGE
                     MRKRYGFIYVDMDDAGNGTLARTPKDSFYWYKKVIASNGEDVE"
     gene            5305..5688
                     /locus_tag="U1099_000220"
     CDS             5305..5688
                     /locus_tag="U1099_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM4866571.1"
                     /translation="MKIEHVAIYTQDLEGMRNFFENYFNATSNQLYHNLKTSFKSYFL
                     TFEDGVRLEIMTRDDVVDKPSQLNYLGLIHLAFSLGSEEAVDELTERLVAAGYLLLSG
                     PRITGDGYYESCVLGFDDIQIELTV"
     gene            5984..7417
                     /locus_tag="U1099_000221"
     CDS             5984..7417
                     /locus_tag="U1099_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM4866572.1"
                     /translation="MSKLPKNFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVP
                     REITAGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEAGLQFYDNLFDECLKYGIEPVVTLSHFELPYHLVTEYGGFRNRKTIEFFVRFAETC
                     FRRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYQEGENREAVMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCKPSDILMAQKAMQKRYYFADVHVHGFYP
                     EHILRYWERKGISVDFTEEDREDLLEGTVDYIGFSYYMSFAIDSHRENNPHYDYLETE
                     DLVKNTYVKASDWEWQIDPEGLRYALNWFTDMYHLPLFIVENGFGAIDQVEEDGMVHD
                     DYRIDYLGAHIKEFIKAVDEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDE
                     GNGSYARSPKRSFAWYKNVITTNGEEV"
     gene            7498..7737
                     /locus_tag="U1099_000222"
     CDS             7498..7737
                     /locus_tag="U1099_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEM4866573.1"
                     /translation="MVGFIAACLTTFGFVPQVIKVVKTKDTESISLGMYMMSVAGMSL
                     WLFHGIIQGDTALIIANSLSVTLAGIILVYKLVYK"
     gene            7961..8299
                     /locus_tag="U1099_000223"
     CDS             7961..8299
                     /locus_tag="U1099_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866574.1"
                     /translation="MESIVGIVVIVVVTVVLVYLLTNMWKIRAVSVGQTDDTTLYFVR
                     FKGVNPPDLANSSVFNQVAKIKCAKGDTYQVTSTVNDVKLRDVIMDEFQLKSSEVVVY
                     YPPRLFFYAS"
     gene            8477..8791
                     /locus_tag="U1099_000224"
     CDS             8477..8791
                     /locus_tag="U1099_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEM4866575.1"
                     /translation="MVKALIICAGGMSSSLIAKKTQTLLEEQGHKVEMNAVGVPEGGK
                     KINAAEYDLYLISPQTKMHFKQFEEAGKKVGKPIVQIPPQAYIPIPMGIEKMAKLVLD
                     NI"
     gene            8838..10811
                     /locus_tag="U1099_000225"
     CDS             8838..10811
                     /locus_tag="U1099_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM4866576.1"
                     /translation="MLSTKEKMILQYLYQHQNVFSTSKVLAEHLSYTDRTIRTYIKKM
                     ASEISERETGFAILSKQGYGYQLRISDEEKYHRFLSENQLVFGVDYSDAENRHKQILN
                     KLIFEEECILFDDLADQLFVSRSTLSHDFKHIREHLASYGLSIESKPYKGVYVVGEER
                     QKRHFIMDYFFSEHFYQNIHHFLSNDKSLALPLSFEELTIVVLDECRDSQLKLSDYII
                     QNLVIHLGLAIQRYQKGFTISPVTIDKEKYAEELAVATKIARRLSHRLEPQNRFPEEE
                     VVYIALHLISKTQLEVEKSSDRLLLRQALFEVLGRYDDEYGYQFSHDFSLIEGLLTHL
                     EVLVERLRHNLHIDNPLLDDIKTTYHDIFDLTQSLMAELSVFHSYSLSESEIAYVALH
                     LMASLERQKEKYKLNVLVICATGYGSAQMLKNRIKNELGQQVSISDVIGYYDLDNQKL
                     QGVDVIMSTIDLSGLVFKVPVITVSVFLTDQEVKEVKQRLADLRPSLQKTQVVERESN
                     LEKYFDQYFSEQRFLVVESAEKEMLLKQMISQLESEGLGNHQEFYKLIEARESLSTLV
                     FDKDIAVPHPLKPISEKHMISVAVIKNGLYWEEGYGQIRLVFLVSPSIYTNEGLSTIT
                     NRLVDLVDLPEVKKDIINSPDFETFKHVFLQRE"
     gene            10822..11136
                     /locus_tag="U1099_000226"
     CDS             10822..11136
                     /locus_tag="U1099_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEM4866577.1"
                     /translation="MNTEELQMAAFGIILSSGNARSVIHEAFAAMREGDYDKAEELLE
                     AANADMLEAHHSQTGLLQEYASGTEVKVEIIMVHAQDHLMTTMTLREVALEMLALYRK
                     VG"
     gene            11151..11663
                     /locus_tag="U1099_000227"
     CDS             11151..11663
                     /locus_tag="U1099_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866578.1"
                     /translation="MSKGMASKKLQKTPEQLKQESKRKSQLFNRYMLFRYSLAVFFFA
                     NLYWLLVQLFQPSLYLILPSLMLIAIIIATAEQFKLYSAEESRLTKTEFVLRAQAILQ
                     GLIVVLVILGHISTLFPTFSNHTSAKLFVIGLQLLGIALVVLNLKRLEQVKQNTDKYY
                     LRFQIIRKYL"
     gene            11683..13032
                     /gene="celB"
                     /locus_tag="U1099_000228"
     CDS             11683..13032
                     /gene="celB"
                     /locus_tag="U1099_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEM4866579.1"
                     /translation="MEEQKGFFGLLDKYLMGPMGKLASYKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVSDIFAASFDKFTPLYMVANTATMGVLSLYFALAIGYEL
                     TKIYAEEEELNLTPLNGALLSLFAFIMTVPQIIFEEGAMSAVSSLEEGAVIVDGWAMG
                     GGVTRFGTTGIFTAIIMAVAAVLLYRMCVKNNWVIKMPDSVPEGVSRGFTALIPSFVV
                     AFFVIFLNGVLVALGTDIFKVIAIPFSFVGNLTNTWIGLVIIYLLTQALWVVGIHGAN
                     IIFAFVSPIALANMSLNAAGERMIVAGEFSNMFVIAGGSGATLGLCIWLATRARSEQL
                     SAIGKAAIVPGIFNINEPLIFGLPIIYNPALAIPFMLAPIASMTVYYFSMKLNLINAV
                     VAQVPWPTPVGIGAFLGTADWRAIVVSIVCAVVAFLVYYPFIRAYDKQLLKEEAANA"
     gene            13438..14904
                     /gene="ascB"
                     /locus_tag="U1099_000229"
     CDS             13438..14904
                     /gene="ascB"
                     /locus_tag="U1099_000229"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM4866580.1"
                     /translation="MTKQFPKGFLWGGATAANQYEGGWNLGGRGPATSDTYIAVDPDK
                     RKDMSHFGKPVSRADVEFALADQEGLYPKRWGSDFYHRYKEDIALFAEMGFKTFRLSI
                     AWSRIFPKGDELEPNEEGLAFYDAVFDELNKYGIEPLVTLSHYEFPLHLALEYGGWKN
                     RKVIEFFVRYAETVFKRYQGKVKYWLTFNEINILGMVGYLSGGLLFEDGKNDLEAMYQ
                     AVHHQFIASSLATKAAHEIDPENKVGCMLARMENYAATCNPDDVLAALKKDQENLFYT
                     DVQVRGAYPSYMKRFFKENNIQVVFEPGDEAILKQYPVDFMSFSYYMTSITRALPDKD
                     KATAGNLILGEANPYLEASDWGWQIDPVGLRITLNKLYDRYQVPLFIVENGLGALDKV
                     EADGSIEDGYRIAYLEKHIQQMYEAIEDGVELMGYTPWGCIDLVSASTSEMSKRYGFI
                     YVDADDQGKGSFDRSRKASFYWYKDVIASNGANVLTEK"
     gene            14913..15515
                     /locus_tag="U1099_000230"
     CDS             14913..15515
                     /locus_tag="U1099_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM4866581.1"
                     /translation="MTKTIYLMRHGETLFNTQKRVQGWSDSPLTERGIAQAQAVGQYF
                     KEQGIVFTSAYSSTQERATDTLKLVTDAPYQQLKGLKEMNFGIFEAREEELLPKHRPG
                     ARSFEDLLVPFGGEDIRAVGSRVKEAILSVAESDPADPILMVSHGAAIWGLVQVLEVT
                     FPPGVFLPNCAICQLTVENEQIRFEKLIDSAHDFKVYDFK"
     gene            15527..16144
                     /locus_tag="U1099_000231"
     CDS             15527..16144
                     /locus_tag="U1099_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM4866582.1"
                     /translation="MNYLYLMRHGQTRFNQQGRIQGACDSPLTELGREQALAAHQYFQ
                     EQGIEFDKIYSSTQERACDTAELVTGRTDYIRLKGLKEQDFGAFEGQQEYLNPPLQGD
                     IGYGDYFVTFGGESYQDVRQRMVETIGGIMEEADNQSVLVVSHGAAIAQFFRQVLTDY
                     PQVRMRNCAILTFTYEDGHYDLQSIVDPVNKENLYQKEGKEKEKK"
     gene            16141..17580
                     /locus_tag="U1099_000232"
     CDS             16141..17580
                     /locus_tag="U1099_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM4866583.1"
                     /translation="MTVTKVFPKGFLWGGATAANQCEGAYDVDGRGLANVDVVPIGQD
                     RAAIITGRKKMFDFEDGYFYPAKESIDMYHHFKEDIALFGEMGFKTYRLSIAWSRIFP
                     KGDELEPNEKGLQFYEDLFKECHKYGIEPLVTITHFDCPMHLIENYGGWRNRKMLEFY
                     ERLCRTLFTRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENVEQVKYQAAHHELV
                     ASAIATKLAHEIDPENKVGCMLAAGQYYPHTCHPRDVWEALQEDRENYFFIDVQARGY
                     YPNYAKKKWERAGIEIEMTNQDLALLKEHTVDFISFSYYSSRVASGDPVEKEKTAGNI
                     FASIKNPYLDASEWGWQIDPLGFRITINSIWDRYQKPLFVVENGLGAVDTPDENGYVE
                     DDYRIDYLRQHVLAMRDAIVEDGVELMGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDN
                     EGNGTLKRSKKKSFEWYKKVIATNGADVG"
     gene            17664..18653
                     /locus_tag="U1099_000233"
     CDS             17664..18653
                     /locus_tag="U1099_000233"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEM4866584.1"
                     /translation="MKYIVNNSNDPAYNIALEAYAFKELTDIDEIFILWINEPAIIIG
                     KHQNAIEEINKEFTDEKGIHVVRRLSGGGAVYHDLNNLNYTIISNKADEGAFDFKTFS
                     KPVIDTLATLGVEANFTGRNDLEIDGKKICGNAQAYAKGRMMHHGCLLFDVDMSVLAS
                     ALKVSKDKIESKGVKSVRARVTNINNELPEKMTVLEFKDAILNQMKQEYPDMDEYVLS
                     EGDLVRIQEIRDTQFATWDWTYGQTPEYTVERSVRYPAGKITTYIKAEKSVIESIKIY
                     GDFFGIGDVSDIEDLLVGTRYEYADVLAKLQEIDTTHYFSRMTTEEVAKAIVA"
     gene            18825..19115
                     /locus_tag="U1099_000234"
     CDS             18825..19115
                     /locus_tag="U1099_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEM4866585.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATIVESKAWE
                     KRRLAYEIKDFREGLYHIVNVEANNDEALKEFDRLSKINGDILRHMIVKLDA"
     gene            19127..19621
                     /locus_tag="U1099_000235"
     CDS             19127..19621
                     /locus_tag="U1099_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM4866586.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQNGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRTAREGQGGGYSAGNSFAGGNDYNSPYQAPAQSTPNFAREESPFGASNPMDISDD
                     DLPF"
     gene            19654..19893
                     /locus_tag="U1099_000236"
     CDS             19654..19893
                     /locus_tag="U1099_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEM4866587.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFISERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALLPFVNED"
     gene            complement(20284..20940)
                     /locus_tag="U1099_000237"
     CDS             complement(20284..20940)
                     /locus_tag="U1099_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEM4866588.1"
                     /translation="MSFTEINGLTKKNQEFIHIATNQLIKDGKSDSEIKELLEEILPT
                     IIEKQKTGVTARNLYGAPSEWAASKTVSEQEKKDQVEYNENPWLMWLDSSLFMLAIIA
                     GINGLMNLFGQGAQYGLLTLFVIGFGVGAGMYLMYHFVYREQIKTGQRPKLLKAIAFL
                     GLATLAWSVVFILAALIPAAFNPVLPPLVTILIGAAAFGARYLLKKKYNIRNAMSPVQ
                     "
     gene            complement(20956..21900)
                     /locus_tag="U1099_000238"
     CDS             complement(20956..21900)
                     /locus_tag="U1099_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM4866589.1"
                     /translation="MKQIFLSTMTDLEEIQTFEPGAWINLVNPSQSESMEVAEHYKID
                     IADLRAPLDAEESSRITVEDDYTLILVDVPILEERNNKTYYITIPLGIILTDDAIITT
                     CLEPLPLFEQFIHRRLRNFFTFMKSRFVFQILYRNAQLYLSALRTIDRKSEEIEKQLH
                     EATRNEELIVLMELEKTIVYFKASLKTNERLVKKLASSSRLLRKYEEDEDLLEDTLVE
                     TQQAIEMADIYGSILNSMTHAFASIISNNQNTIMKTLALVTIVLSIPTMVFSAYGMNF
                     KDNIIPFNDIPYAFWWIILFAFAISLSLTFYFIRKRWF"
     gene            22739..25564
                     /gene="uvrA"
                     /locus_tag="U1099_000239"
     CDS             22739..25564
                     /gene="uvrA"
                     /locus_tag="U1099_000239"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEM4866590.1"
                     /translation="MQENIVIHGARAHNLKNIDVTIPREKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGTA
                     TEINDYLRLLYARVGVPYCINGHGAIAASSVEQIVDEVLELPERQRLQILAPIVRKKK
                     GQHKTIFEKVQKDGYVRVRVNGDVYDVSEVPELSKSKAHNIEVVVDRIVIKEGIRSRL
                     FDSIEAALRIADGYVIIDTMDDKELLFSEYYACPVCGFTVPELEPRLFSFNAPFGSCS
                     DCDGLGMKLEVDTDLIVPDASKTLRDGALAPWNPISSNYYPQMLEQAMNHFGVDMDKP
                     FEELTEEEKNLIFNGSDGKEFHFHYENEFGGVRDIDIPFEGLITNINRRYRETNSDYT
                     RTVMKTYMNELTCGTCHGYRLNDQALSVKVGGEQGLHIGQLSDLSVADHLEVIEHLTL
                     SENEATIATPIVKEIKDRLSFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADWLIDIGPGA
                     GVFGGEIVASGTPAQVAKNKKSITGQYLSGKREIPVPLERRVGNGRFLEVTGAKENNL
                     QDVTVRFPLGKFVAVTGVSGSGKSTLVNSILKKAIAQKLNRNSDKPGKFKSISGIEHL
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVFVPCEVCHGHRYNSETLEVHYKEKNIAQVLDMTVNDAVE
                     FFKHIPKIERKLRTIQDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTEDIAQLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDMGPEGGVGGGT
                     VVATGTPEEVASNPASFTGQYLKMKLK"
     gene            25663..26724
                     /locus_tag="U1099_000240"
     CDS             25663..26724
                     /locus_tag="U1099_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM4866591.1"
                     /translation="MQARVEKFEAKLAQSDVDGILVTGQNNIYYLTGFWGTEATVFIS
                     GKRRLFVTDSRYTLIAKASVKGFDIIESRFALEEIAKVIKEDGLEKIGFDSEVTYGFY
                     QSLTSIFEGYQLVAMSNFIEDLRMIKDEKEIATIRRACQISDQAFIDVLDFIKPGQTT
                     EMDVNHFLDHRMRQLGAEGASFEFIVASGYRSAMPHGRASEKVIQAGETLTLDFGCYY
                     QHYVSDMTRTIHIGHVTDQEREIYDVVLRANKALIEQAKEGVTYREFDAIPREIINAA
                     GYGANFTHGIGHGIGLDIHEYPYFGKSDETIKAGMVLTDEPGIYLDDKYGVRIEDDLL
                     ITENGCEVLTLAPKELIVI"
     gene            26788..27345
                     /gene="efp"
                     /locus_tag="U1099_000241"
     CDS             26788..27345
                     /gene="efp"
                     /locus_tag="U1099_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEM4866592.1"
                     /translation="MIEASKLRAGMTFEAEGKLIKVLDASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYQMDDTAYFMNTETYDQYEIPVATIKEEL
                     LYILENSDVKIQFYGTEVIGVTVPTTVELVVAETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIEAGQKLVINTAEGTYVSRA"
     gene            27373..27765
                     /locus_tag="U1099_000242"
     CDS             27373..27765
                     /locus_tag="U1099_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM4866593.1"
                     /translation="MTTEYQGEIVIAPRVLEVITGIAAAKVDGVHSLQNKRVADSLSK
                     TSLNKGVYLETDEEGRVTADIYVYLEYGVNVPAVSMDIQRAVKTAVYNYAEVQVAAVN
                     IHVSGIVPDKTPKPALKDLFGEDFLDEE"
     gene            27755..28174
                     /gene="nusB"
                     /locus_tag="U1099_000243"
     CDS             27755..28174
                     /gene="nusB"
                     /locus_tag="U1099_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEM4866594.1"
                     /translation="MKNNRHALRKCALQAIVSLEFGQEPVQAAQFSYLYDREEEQEGV
                     EIPLFLLNLVNGVADYRDDLDKELSSRLKAGWTLDRLTLIDKNIMRLGLFEILHFEET
                     PDRVAVNEAIELAKEFSDESSAKFVNGVLSQFIKEEA"
     gene            complement(28253..29218)
                     /locus_tag="U1099_000244"
     CDS             complement(28253..29218)
                     /locus_tag="U1099_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM4866595.1"
                     /translation="MVAKLTDVAQLAGVSPTTVSRVINKKGYLSEKTIRNVEEAMREL
                     GYKPNNLARSLQGKSAKLVGLIFPTINNIFYSELISHLEKELFDRGYKTIICNSQHES
                     DKEREYLEMLAANQVDGIISGSHNLGIQDYDRVVAPIIAFDRNLSPSIPIVSSDNFAG
                     GVLAAQTLQKAGCHNPLMITGNDDSNSPTGLRQVGFTSILNKAEVFHISSDFSPVRKE
                     MEIRAILEKQKPDGIFVSGDATAMLVWNVIRSMKLSIPEDIKLIGYDGTSFIENYYPQ
                     LTTIKQPLTEIARLLVELLVDKIEGKKLTKTDYILPISLLAGASI"
     gene            complement(29199..30653)
                     /locus_tag="U1099_000245"
     CDS             complement(29199..30653)
                     /locus_tag="U1099_000245"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEM4866596.1"
                     /translation="MAFTTEERYRAYADWTPEYIEKIKKNTENSPWRTNFHIETPHGL
                     LNDPNGFSYFNGKWTLFYQYFPFGAAHGLKSWVHTESTDLVHFEKTGTVMYPDTPLDS
                     HGAYSGSAMEFGDKLFLFYTGNVRDENWVRHPYQIGALMDKDGKIEKIDKVLIEQPED
                     TTDHFRDPQIFNYKGQYYAIVGGQNLDKKGIVKLYKAENNDYLNWSYVGDLDFANDMT
                     AYMMECPNIAFVGEQPILLYCPQGLDKEVLDYSNIYPNMYKIGQSFDPETASIVEPSE
                     LINLDYGFECYATQAFNAPDGRTLSVSWLALPDVEYPTDAYDYQGCLSLVKELTIKDG
                     KLYQYPVDAITSLRKEAQPFSAQKETNNVYELELDFSAGTKHEIVLFANEAEKGLVLS
                     VDTEQGQITLDRGNCGQPFALDFGTSRSCEISPGALTANIFIDKSVFEIFINKGEKVF
                     SGRVFPDADQSGIVITTGNPTGTYYHLDYGRKTN"
     gene            30850..32796
                     /locus_tag="U1099_000246"
     CDS             30850..32796
                     /locus_tag="U1099_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEM4866597.1"
                     /translation="MDYKKVSAEVIEAVGKDNLVAAAHCATRLRLVLKDESKVNQKAL
                     DDNADVKGTFSTNGQYQVIIGPGDVNFVYAELIKATGLKEVSTDDLKQIAAQDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPKSIEEMYPAIQGISAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDIFGFQVAQT
                     NYAYQVIPVLAAVWLLATLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLT
                     VSDAITNAIVWLYDTTGALGMSIFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGY
                     GDYIFVVASMANVAQGAATLAIYFLTKNAKTKGLSGSAAVSAFLGITEPALFGVNLKY
                     RFPFFCALAGSAIGALFAGLTHVIAVSLGAAGFIGFLSIKASSIPMYVVAEVIAFAAA
                     FAFTYVYGKTKASAVFADEAATAAAAAVTASVVETSEEVEETAEIFTAQETLVSPLSG
                     DVVALENVNDPVFSSGAMGKGLAVKPSEGVVYAPADAEVTIAFETGHAYGLKTASGAE
                     LLIHIGIDTVSMNGNGFEKLVAAGDKVKAGTPIAKFDATKIAEAGLDDTTMIIVTNTA
                     DFAEVSPLAEGTIAHGVEFLKVAK"
     gene            32924..33808
                     /locus_tag="U1099_000247"
     CDS             32924..33808
                     /locus_tag="U1099_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM4866598.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFQVVEKTQFPTTTPYETIERT
                     VEFFKRYEDRLAGIAIGSFGPIDIDQNSQTYGYITSTPKPHWSNIDLLGLIAKEFNIP
                     FYFTTDVNSSAFGETLVRKGVKSLVYYTIGTGIGAGAIQNGEFIGGLGHTEAGHTYVA
                     LHPYDVKHEFKGMCPFHNGCLEGLAAGPSLEGRTGIRGELIELNSDVWDVQAYYIAQA
                     AVQATLLYRPQVIVFGGGVMAQEHMLNRVREKFVGLMNDYLPTPDVKEYIVTPAVAEN
                     GSATLGNFALAKQVSRNS"
     gene            34422..>35541
                     /locus_tag="U1099_000248"
     CDS             34422..>35541
                     /locus_tag="U1099_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEM4866599.1"
                     /translation="MIPIQQHGKRWLILTLTLSTSVLALASIGASQPVVFADENSHLQ
                     SQKNINKIEVLDWKTFSKKLSEYSSDQRQFHVLKLGFENRLGTLSTWDELEEFGKNNN
                     FLVINGKVTQNIHDFPHILVMNKGDVIAHNEEDYHNQMRELRFSGNGDLHNSMEPKRI
                     HALFKIELDSNKRQLLNAAGLGTAENSLKNINGMTIYSHGLTVDNKYYEDYSKYTHNS
                     VKNINVTKERFIANDDLIHKLIESSEAMKQSSERDKVKAFVQYVANHTTYDWEAANKA
                     VQNYADINYYLGSDLFAVTERQKAMCVGFSTTAARAFNMLGLPAYVVVGKNAEGVPHA
                     TARVYYDKKWHTIDGTGFITDITDSNKHQRSAKYSEKHF"
BASE COUNT        10292 a         6698 c         8039 g        10512 t
ORIGIN      
        1 ttcccagaaa agaaaggagc cgaaaaatgg ctaaggatta tacagattta gctacagata
       61 ttgtagctca tgttggtgga aaagaaaaca tcagcagttt gaaacactgc gtgactcgtc
      121 ttcgttttgg tctcaaggat gagagcaagg ctgatacaga ctacttgaag gcgcgtgatg
      181 gcgttgtgac agtagttcaa gcaggtggtc aataccaagt ggttatcggc aaccatgtac
      241 ctgatgttta tgcagcggta caaaaagttg ctggtatctc aggggatggc gccttagata
      301 ttgatgaagg tgacggaccg aagggaaatc tgtttgaccg atttgttgac ctagtttcag
      361 gtgttttcca gccttcttta ggagtgcttg cagctgcagg gattatcaaa ggattggtat
      421 ccatcctagc cgctacaatg ggtttgaaca atgataatag cgccctatat gctattttaa
      481 acacagctgg agatggattc ttccaattct tgccaatcgt cttagctttc aatgctgcac
      541 gaaaattcag aatgaatgaa tttactggta tggcaattgc tatggcattg gtttacccaa
      601 ctctgccagg gacatttgcg actctaaaag aagcgggtct aaataatgtt tttggcatac
      661 catttgtact tccagcagca ggtagctact tgcaaactgt gatgcctgtc ctacttgctg
      721 tctggattgc ttctcatatt gagaaattca tgaagaaaat tacgcctgac gtagttaagg
      781 tcttcattgt accgtttgtg acaattctaa tttcagttcc attgacattc ttggttgtag
      841 gtcctgtcgc gaactacgct tcagatcttt tgtctgcagg ctttactgcg gttatgggct
      901 ttagtccact actttacggt attctacttg gtgctttatg gcaagtaatg gttatgttcg
      961 gacttcactg ggccttggta ccacttgcaa tacttcagtt ttctcaaaac ggatggtcaa
     1021 atattttgtt agctgcagcg cttccaaact ttactcaaac tggtgttctt ttggcaatta
     1081 tgatgaaaac caaagaatca aaagtgaagt cagttgcgat gcctgcattc atttcttcaa
     1141 tctttggtgt cactgagcct gctatctatg gtgttactct tcctatgaaa acaccattct
     1201 acatttcttg tgcagtgtca gcagtaattg gtgcaggtct ctcattcttt gatttgaaaa
     1261 actatgctat gggtgctctc ggtatcttta tgtatccagg ttatgttagt cctgaactag
     1321 gtgtgactcc aatgtggatt atgatgggct tctctttggc ggcaattgtt ttatcattcg
     1381 ctatccaaat ggctcttcca gttccttact tatatggaga accaactgaa aaaaagtccg
     1441 ttgaagaaac aaaggagtct gttccagaat taaaagaaat taaacaagag atcattgcta
     1501 gtccactaat cggtaaagta gttaaattag aagatgttcc tgatgcagtc tttgcttcag
     1561 gagctatggg gaaaggaatt gctatcgatc ccctagatgg aatccttgta tcaccagcga
     1621 aggctgaagt cactcttcta ttccctacca agcacgccat tggtcttcgg acggaaaatg
     1681 gggcagagct actcatccat atcggtatgg acacagtctc tctagctggt aaaggctttg
     1741 aatccttcgt ccaagtaggt gaccaagttg aagcaggaca aaaattgctg gaatttgacc
     1801 tccaccaaat caaagcagct gacctaccgg tcatcactcc aattatcgtg accaatacag
     1861 ctgactatga ggacatcttg gtaacccaag aaagccaaat caacagtggt gattacctgt
     1921 tgacaacggt caaataaaaa ataaagtggt tggcagtgcc agtcactttt ttgaatcttg
     1981 tttagcgttg aagaaaatcc ttatcctgct ctggaatgcc cgcctcatca cttttatagc
     2041 gttcagcggt gacagttagc tggtacttac tacgtaagtc taggatctcc tgcatcattg
     2101 gagaagcatg gtggatgtcc aaagctgctt ggtctgtcca gctatcaacc aagaggattg
     2161 aatgaggatc atcgagaggt gcaaagtatt gataacgtgt atttccagcc tgctggcgaa
     2221 tgcggtcggc aatgccacta gcttccattt cctgagcgaa agcacgtgca ttgtcagctg
     2281 gtccggtgta gtagaggtgc atggtaatca tagtataaga tatagagggc gctaagcggc
     2341 cacagacaaa atatagtctt ttagtttact tttagtacta ggcaatgagc tgcaggcagt
     2401 actgaagtac ggcaaagcga atgaacgaag tattaaaaga aaaactaaat gacgtaggaa
     2461 acagaacgac gatgcaagcg tccagttctc gtttatcttt ttggtttagt ccgtagcccg
     2521 tattcagttc aactaaatac agaggcccta gtcctttcta ttatttatca aagttagtac
     2581 aggtcctatt cttccgcaat tgtttgcgat agctgctagg ggtggtatgg tagtattgtt
     2641 tgaactggcg gttgaagttg gacaggttat taaagccaac atcggtcgca atttcaagca
     2701 caggcttgat agtgtttgtc aggagctcgg tagctgctcg cagtcggact tggatgataa
     2761 attccataca ggaagttcct gtttgttgct tgaagattgt cataaaatgg gtttttgtat
     2821 agcccatttg ctctgctagc tgttcgatgg tgatattttc tgcataatgt tgttggatgt
     2881 attcaatcaa caaccgcagt ttttcatttt tccgatacat atcatcggta ttttttcgga
     2941 cgatgtagcg gtagcggtag agcaggtaga agaagtcaaa gagtcggctc ttgagaggta
     3001 gctcaaagta aggcggctgg tgtctgacca actcaaaaat ggtgagcagg cattgtctaa
     3061 tctctgcgta gccaggatct tggggctgga tgcgtggaat acacttgaaa acatctgtct
     3121 gtaagggttg taggtagagc atactggtct gatctaggtg agacgccccc agcatatcca
     3181 agtgaaagag aagggtatca gtttcgtgcc cccgttgctc aatcggatga atagaatgcg
     3241 cagcattggg tcgaatcagg ataatatcac cagcttgact attgaaatag tcatagtcga
     3301 tatggtaacg ggctgtccct ccgtaaacat agttgatttc aaattcattg tgccaatgaa
     3361 agagaatgtc aggaatgcca tccgtgacat aggtattgta atacttatag ggtaacaaac
     3421 gactatcgta gtcaatggta tgtttaaact gtttatccgt taatggcggt tgcatacttt
     3481 cctccttttc gtaggatagt atcattatag catattattt gagaagaaaa gggtttcaaa
     3541 tctgtttata ctatatttgt tagaaaaaaa taagggagaa gaaacatgtc acgttttcca
     3601 aaaaatttcc tctggggagg agctatcgca gccaaccagg cagaaggtgc ctataatgtg
     3661 gacggacgcg gtctagtcca gacagacgtt acaacaggag gatctgttaa cagcccccgc
     3721 tatacgactt acattgataa ggacggcaag ccaggtaaat atgcttctat ggggcatgct
     3781 ggcggtcatc tgccagaagg ggcgacctat gccgtcctag aagaccacta ttatccaaac
     3841 cacaaggctg tggacttcta ccaccgctat aaagaagata tcaagctctt cgcagaaatg
     3901 ggttactccg ttttccgctt gtcaatttca tgggcacgga ttttcccaaa cggtgatgat
     3961 gcagagccaa accaagcagg tcttgatttc tatcgttctg tttttgaaga atgccgcaaa
     4021 tatggcattg agccattggt ttccatttgg cacttcgata cacctttgag cttggaacaa
     4081 cgctatggtg gttggaccaa tcgtaagttg attgatttct atgtccgcta tgctgagaca
     4141 atttttaatg aatataaggg gttggtgaaa tattggatta ccttcaatga gattaacaac
     4201 actatcatgt ttttggatat gtttagtgaa ccagaggatt ctgattacca agaagcctac
     4261 caacacttac atcaccagtt tgttgcctct gccaaggctg taaaattagg tcatcaaatt
     4321 gatccaagtt atcgattcgg caacatgatt tgtggaatca ccttctaccc aggaacctgt
     4381 gaccctgcgg atattttaga aaatcaacac aaatgggaac agaatatttt ctattgtagt
     4441 gatgcacaag ccaaaggtcg ctatggttcc tatgcaaaca gactgtggaa ggaacacaat
     4501 gttgtattgc aaatggaaga gggagaccta gaaatccttg ctgaaggcaa ggtggatatg
     4561 tataccttct cttattacat gtcaacagtt gtgaccactc ataaagtgga agacacggtt
     4621 tctggaaact tctcaatcgg tgccaagaac ccttacttga cctattcaga ctggggttgg
     4681 gctcaggatc cgaccggtct tcaatactat cttgagaaaa tctacgaccg ctatgaaatt
     4741 ccgatgatga ttgtagaaaa tggtttggga gctttggata cagttgaaga agacggctct
     4801 attcacgatg attaccgtat cgactaccat cgcgcccatg tcaatgctat ggcagatgct
     4861 attgccaatg gtgttgattt gattggctat acgacttggg gctgtattga cctagtatca
     4921 gcaggaacag gtgaaatgcg aaaacgctac ggctttatct acgttgatat ggatgatgct
     4981 ggaaatggga cacttgctag aacaccaaaa gattcattct actggtacaa gaaagtcatt
     5041 gccagcaatg gtgaggatgt agaataggaa aacggcaatg tgccgtttca gattgaagaa
     5101 gaagtccatt ttggacaatt ttcttcaatc tttttttctt tatgatgaaa attaaaaact
     5161 ctcagaaacg ccgaaatatc aatgttccta agagtttaat gacttgttat ctttcacaaa
     5221 cttcttcaat tttttatatt tttaggagtt tgtctaggtt ctgagacggc actttgctgt
     5281 tttctcttag ggaaaggttt gaaaatgaaa atagaacatg tagcaatcta cactcaggat
     5341 ttggaaggta tgcgtaattt ctttgaaaac tacttcaatg ccacatccaa tcaactctac
     5401 cataatctaa aaaccagttt caagtcctat tttcttacct ttgaagacgg ggttcgtttg
     5461 gaaattatga ccagagacga tgtggtcgac aaaccaagtc aactcaatta tctaggactt
     5521 attcatttag cctttagtct agggagtgag gaagctgtgg atgagttaac agagcgactt
     5581 gttgctgcag gctacttgct tcttagtgga ccacgaataa ctggggacgg ctactatgaa
     5641 tcgtgtgtgt tgggatttga tgatatacaa attgaattaa cagtgtaatg ttggcaagta
     5701 atactgctag atcaatgttt aacggattag cttgcaagta tcattcatta gcgatttcct
     5761 tactgttacg gaaaaaactg caatggtgat tgatgtaaac gtttgctaaa ctaataacta
     5821 taggaacctg tattcacaag tagggtgctt gtatataaga gatgtttttg agggaatact
     5881 gactgtattt tgaaaaaaaa ctaacaatga gtagcttaaa aattagtctt agatggaaat
     5941 tctgaactgt aaatacaggt tctaaaatat acaggaggca attatgtcta aattacctaa
     6001 aaatttttta tggggtggag cagtagcagc tcaccaactt gaaggtggct ggcaagaagg
     6061 tggaaaaggg atttctgtag cagatgtgat gactgctggg agacacgggg tacctagaga
     6121 aattacagct ggcgtattag aaggaaaata ttatccgaat catgaagcga ttgatttcta
     6181 tcaccgctat aaagaagata ttgcactctt tgctgagatg ggcttcaagt gtttccgtac
     6241 ttcaattgca tggacacgta tttttccaaa gggcgacgaa ctggaaccaa atgaagctgg
     6301 cttgcagttt tatgataact tatttgatga gtgtttaaaa tatgggattg aacctgttgt
     6361 taccctatca cactttgaat tgccttacca tctggtaact gagtatggtg gttttagaaa
     6421 tcgtaaaacc attgagttct ttgttcgttt tgcagagacc tgcttccgtc gttacaaaga
     6481 taaggtcaaa tactggatga cctttaacga gattaataac caagcaaatt atcaggaaga
     6541 ttttgcacct tttaccaact caggtatcgt ttatcaagaa ggtgaaaatc gtgaagcagt
     6601 tatgtatcaa gcggcccatt atgaattagt tgcctctgca cgcgctgtta aaattggaca
     6661 tgaaattaat cctgactttc aaattggttg tatgattgcc atgtgtccga tatatcctgc
     6721 aacctgtaaa ccatcagata ttttgatggc acagaaggcg atgcaaaaac ggtattactt
     6781 tgcagatgtt catgtgcatg gtttctaccc tgagcatatc ttgcgctact gggagagaaa
     6841 aggtattagc gttgatttca cagaggaaga tcgcgaagat ttgctagaag gaacagttga
     6901 ttacatcggt ttctcatact atatgagttt tgctattgat tctcaccgcg agaataatcc
     6961 tcattacgat tatcttgaga cagaggactt ggtgaaaaat acctatgtga aggcttctga
     7021 ttgggaatgg caaattgacc cagaaggatt gcgctatgca ttaaactggt ttacagatat
     7081 gtatcatctg ccacttttta tcgtagagaa tggctttggt gcgattgacc aagtcgaaga
     7141 agatggaatg gtgcatgatg actaccgtat tgattatctt ggtgctcata tcaaagaatt
     7201 tatcaaggct gtcgatgagg atggagttga cttgatggga tatacgccgt ggggttgtat
     7261 tgacctggtg tcagcaggga caggtgaaat gcgcaaacgc tacggtttta tctatgttga
     7321 taaagatgat gaaggaaatg gtagttatgc gcgttctcca aaacgctcat tcgcatggta
     7381 caaaaatgtc atcacaacaa atggtgagga agtttaatga gaagacctct gtagcgtaat
     7441 agctacatgg gtcttcattt atgtttttag tattggtata ttaaaggaga aaattgtatg
     7501 gttggtttta tcgcagcttg cttaactacc tttggatttg tacctcaggt tatcaaggta
     7561 gttaagacaa aggatacaga gtcgatttcg ttaggaatgt atatgatgtc tgtggcaggg
     7621 atgtccttat ggttgttcca tggtattatt caaggagata cggccctcat tattgctaat
     7681 agcctatctg taacattagc aggtattatt ttggtctata aattagtcta caaataatgc
     7741 actattccat cgataaaaga atttttagca acagtgaaaa acattgaaaa tgcttgactt
     7801 tttctgtgaa aggaatataa tgatgacaaa taaatattgg aagtgtgatt actatgttat
     7861 tcgttccgct ttttttaagg tttaaccttt ttaaaccacc aagataagcg ttttttctag
     7921 ttgtttgtaa gtgtatcgta tcttggaaaa gaggtttatt atggaatcta tagttggaat
     7981 agttgtcatt gtagttgtta cggttgttct ggtttattta ttaactaata tgtggaaaat
     8041 cagagcggtt tcagttggtc aaacggatga cactacgcta tactttgtaa gatttaaggg
     8101 ggtaaatccg ccagacttag caaatagtag tgtttttaat caagtagcca aaatcaaatg
     8161 tgctaaagga gatacctatc aagtcacttc aacagtcaat gatgtgaaac tgagagacgt
     8221 gattatggat gaatttcagc tcaaatcttc tgaggttgta gtttactatc ctcctcgtct
     8281 tttcttttat gcatcatagt cgcaactctt cggagttgct atttttttgt ttctggagag
     8341 tagcggaaaa gcggtttatt ttagtagcga aatttccgta gtggtaagga aatacttcct
     8401 atggaaaatg taagcgtgtt catgtaaaat gaaatcataa caaaagtaaa taataaaata
     8461 gttggaggaa aatcctatgg taaaagcttt gattatctgt gcaggaggca tgtcttcttc
     8521 acttattgct aaaaaaacac aaacactttt ggaagaacaa ggtcataaag tagaaatgaa
     8581 cgctgttggt gttccagaag gtggtaaaaa aattaatgcg gcagagtatg acttgtactt
     8641 aattagcccg cagacaaaaa tgcactttaa gcaatttgaa gaagcaggta agaaggttgg
     8701 gaagccaatt gtccaaatcc caccacaagc ttatatccca attccaatgg gaattgaaaa
     8761 aatggctaaa ttggtcttgg ataatatcta gttctttgtt accatagatg gtatattgat
     8821 tcgaaaagag gatagttatg ctaagtacta aggaaaaaat gatattgcag tatctttacc
     8881 aacaccagaa tgttttttca accagtaaag tattggcaga gcatctctct tatacagatc
     8941 gcacaattcg aacttatata aaaaaaatgg catccgaaat atctgagaga gaaacaggtt
     9001 ttgcgatttt atctaaacaa ggttatggct atcagcttcg aattagcgat gaggaaaaat
     9061 accatcgttt cttatctgaa aaccaactag tatttggggt ggattatagt gatgcagaaa
     9121 atcgccataa acaaatcctg aataaattga tatttgaaga agaatgtatc ttatttgatg
     9181 atttggcgga ccagttgttt gtcagtagat caaccttgtc tcatgatttc aagcacatta
     9241 gggagcatct tgcttcttat ggtttgagca tcgagagtaa gccctataag ggagtctatg
     9301 tagttggtga agaacgtcaa aaacgtcact tcatcatgga ctatttcttt agtgagcatt
     9361 tttatcagaa tatccatcat tttttatcca atgataaaag tctagcctta cctctatctt
     9421 ttgaagagtt gacaattgta gtcttagatg agtgtcgtga tagtcagttg aaactatctg
     9481 attatattat ccaaaattta gttattcatt taggtttagc tatccaacgc tatcaaaaag
     9541 gttttacaat ttctcctgtc accatcgata aggaaaagta tgctgaagaa ttggcagtag
     9601 ctacaaagat tgctaggcga ttatcccatc gactggagcc tcagaatagg tttcctgagg
     9661 aagaagtggt ctacattgct ctacacttga tttcaaaaac acaattggag gtggagaagt
     9721 catcggaccg cctcttgttg cgccaagcac tttttgaagt tttagggcgt tatgatgatg
     9781 aatatggtta tcaattttca catgattttt ccttgataga aggtttgcta acacatctgg
     9841 aggttctcgt agaacgactc cgtcacaatc tccatattga taacccccta cttgatgata
     9901 ttaaaacaac ttatcatgat atcttcgatt tgacacaatc actaatggct gaactttccg
     9961 tttttcactc ttatagcttg tccgagtcag aaattgccta tgtagctcta cacttgatgg
    10021 cttccttgga acggcaaaaa gaaaaataca aactaaatgt tctggttatc tgtgcaacag
    10081 gctacggtag tgctcagatg ctgaaaaata ggattaaaaa tgaacttggg caacaggtgt
    10141 ccatatcaga tgtcatcggt tactatgatt tagataatca aaagttgcag ggggtagatg
    10201 tgataatgtc aaccatcgac ttatctggtt tggtgtttaa ggtccctgtt ataactgtca
    10261 gtgtcttttt aacggatcag gaagttaagg aagttaaaca acgattggcg gacttgagac
    10321 ctagtctcca aaaaacacag gtagtggaaa gagagagtaa cttggagaaa tactttgatc
    10381 agtacttctc agaacaacga tttttagttg ttgagtcagc tgaaaaagaa atgttattaa
    10441 agcaaatgat ttcacagttg gagagcgaag gtcttggaaa tcaccaggaa ttttataaat
    10501 taattgaggc gcgtgaaagc ttgagtacat tggtatttga taaagatata gctgttcctc
    10561 accccctaaa acccattagc gaaaagcaca tgatttctgt tgcggttata aaaaatggtc
    10621 tatattggga agaaggctat gggcagatta gattggtttt cttagtgagt ccatctatat
    10681 atacaaatga aggtctatca accattacca atcgattggt agatttggta gatttaccag
    10741 aagtcaagaa agacataata aactctcctg attttgaaac attcaaacac gtttttttac
    10801 aaagagaata gaaggtgaca tatgaataca gaagaattac aaatggctgc atttgggatc
    10861 atcctcagta gtggcaatgc aagaagtgtt atccacgaag cttttgctgc tatgagagaa
    10921 ggtgattatg ataaggcaga agagttattg gaagccgcca atgctgacat gcttgaagcc
    10981 caccattctc aaacgggtct cctacaggaa tacgcaagcg gtactgaagt gaaggtagag
    11041 attattatgg ttcatgcgca agatcatttg atgacaacaa tgactttacg tgaggtagcg
    11101 cttgaaatgt tagctttata tcgtaaagtt ggttagaaag aggatatggg atgagtaagg
    11161 gaatggcaag taaaaagcta cagaaaacac cagaacaatt aaaacaagag tctaaacgca
    11221 aaagtcagct ctttaatcgc tatatgctgt ttcgttatag tttagcagtg ttcttctttg
    11281 caaatctata ctggcttctg gttcaattat ttcaaccaag tttgtatcta atcctgccct
    11341 cgcttatgtt gattgcaata atcattgcga cggcagaaca atttaagttg tatagtgctg
    11401 aggagtctcg attgacaaag acagaatttg tattaagggc acaagcaatc ttgcaagggt
    11461 taattgtggt tttagtgatt ttgggtcata tctcaaccct ctttccaact ttttcaaacc
    11521 atacatctgc taaactcttt gttattggct tgcagttgtt aggtattgct ttagttgttc
    11581 tcaatttaaa aaggttggag caggtaaagc aaaataccga taaatactac ctacgttttc
    11641 aaataataag aaaatatctt taaaatttta ggaggtcttc tcatggaaga acaaaaaggg
    11701 ttctttggac tattagataa atatctgatg ggaccaatgg gtaaactggc atcttacaaa
    11761 gttgtacgtg ccatcacagc tgcaggtatg gctgccgtac catttacgat tgttggttcg
    11821 atgttcttgg ttttcagtat cttaccacag gcattttcat tctggccaat cgtttcagat
    11881 atttttgcgg catcttttga taaatttacc ccactctata tggttgccaa tactgctact
    11941 atgggggtgc tatcacttta ttttgcactt gctattggat atgaattgac aaaaatttac
    12001 gctgaggaag aagaacttaa tttgactccg cttaatgggg cgctcttatc cctctttgct
    12061 ttcatcatga ccgttcctca aattattttt gaagaaggcg cgatgtcggc agtcagctct
    12121 cttgaagaag gcgcagtaat tgttgatggt tgggcaatgg gtggtggcgt aacacgcttt
    12181 ggtacaacag gtatatttac ggcaatcatc atggctgttg cggcagttct gctatatcgg
    12241 atgtgcgtga aaaataattg ggttatcaaa atgcccgatt cagtaccaga gggtgtatca
    12301 cgcggattta cagctttgat tccttcattt gtcgtagcct tctttgtaat tttcttgaac
    12361 ggtgtattgg ttgcgcttgg tactgatata tttaaagtta ttgcaattcc atttagcttt
    12421 gttggaaatt taacaaatac ttggattggt ttagttatta tctacttgtt gactcaggca
    12481 ctttgggtag taggtattca cggtgcaaat atcatctttg cttttgttag tccaattgca
    12541 cttgcaaata tgtctcttaa tgcagctggt gaacgaatga ttgttgcagg ggagttctca
    12601 aatatgtttg tcattgcagg tggttctggt gcaacacttg ggctatgtat ttggttagct
    12661 actcgtgctc gttctgaaca attgagtgcg attggtaaag cagcaattgt tccgggtatt
    12721 tttaatatca atgaaccatt aattttcggt ttaccaatta tttataatcc tgctctagca
    12781 ataccattta tgcttgcacc aattgcatct atgacggtat attacttctc tatgaagttg
    12841 aatcttatca atgcagttgt tgctcaagta ccatggccaa ctccagttgg tattggtgcc
    12901 ttccttggta cagctgactg gcgtgcaatt gttgtttcaa ttgtttgtgc agttgttgcc
    12961 ttccttgttt actatccatt cattcgtgca tacgataaac agttgctaaa agaagaagcg
    13021 gcaaatgctt aaactagaga aactccttga cttcttctaa gtccaaggag tttttctata
    13081 agagaatatc aggagacatt gttagataaa cataagatag tatcatattt gctttcttct
    13141 ttctaaatca gaggagctga ggtagggcaa gagaagaaga ttttttatag ctgacttgat
    13201 gaagcgacta tcaatacata tggaggctta aaaatatctc tactactttt gctaatactg
    13261 atcgagagaa aacctctccc ttgttccaag ttttaattgg gatattatct aatgttttaa
    13321 gaatagtgat aattggaaat agcatagaat agtatcagtt tggcatatta tcacgtagga
    13381 atgaaggggg ttcttttgct attatatagc taataaagtc agaaggagta ttgtgctatg
    13441 acaaaacaat ttccaaaagg atttctttgg ggcggtgcaa cagcggccaa ccaatatgaa
    13501 ggtggttgga atttgggagg tcgtggtcca gccacttccg atacctatat agcggtcgat
    13561 ccagataagc ggaaggatat gagtcacttt ggtaagcctg tctcgcgggc tgatgtggaa
    13621 tttgccttgg cagatcaaga aggactctac ccaaaacgtt ggggttctga tttctatcat
    13681 cgctacaagg aagacatcgc cctctttgca gaaatgggct tcaagacctt ccgcttgtcg
    13741 attgcctggt ctcgtatttt tccaaaagga gacgagttag aaccgaatga agaaggactg
    13801 gccttttatg atgcggtctt cgatgagctg aataaatatg gtatcgagcc actggtgacc
    13861 ctatctcact atgaatttcc tcttcatctt gctctagaat acggtggctg gaaaaaccgt
    13921 aaggtcatag agttctttgt tcgctatgcc gagacagtat tcaaacgcta tcagggtaaa
    13981 gttaagtatt ggctgacttt taatgagatc aatattttgg gcatggttgg ttatctatca
    14041 ggcggtcttc tcttcgaaga cggaaagaat gacctagaag ccatgtacca agcagttcac
    14101 catcagttta tcgcttctag tttggcaaca aaagctgccc atgaaattga cccagaaaac
    14161 aaggttggtt gcatgctggc acggatggag aactatgcag ccacctgcaa tccagacgat
    14221 gtcttggcgg ccttgaaaaa agaccaggaa aatctcttct atacagatgt gcaggtgcgt
    14281 ggagcctacc caagttatat gaaacgcttc ttcaaagaaa acaatatcca ggtcgtgttc
    14341 gagccgggag acgaagcgat tttgaaacaa tatcctgttg atttcatgag tttttcttac
    14401 tatatgacat ccatcacccg tgctcttcca gacaaggaca aggcaacggc aggtaatttg
    14461 atactaggag aggccaatcc ttatctggaa gcgtcggact ggggctggca gatagaccct
    14521 gtcggacttc gtatcaccct caacaagctt tatgaccgtt accaagttcc cctcttcatc
    14581 gtggaaaatg gtctgggagc cttggataag gtggaggcag acggcagtat tgaggacggc
    14641 taccgtattg cctacctaga aaaacacatc cagcaaatgt atgaagctat tgaagacggt
    14701 gtggaactta tgggctatac cccttggggc tgtatcgatc tggtttctgc ttcaaccagt
    14761 gagatgtcca aacgctacgg ctttatctat gtggatgcgg acgatcaagg aaagggcagt
    14821 tttgaccgat cacggaaggc atcattctac tggtataaag atgtcattgc cagcaatgga
    14881 gccaatgtat tgacagaaaa ataggaggtc ttatgacaaa aacgatttat ctcatgcgtc
    14941 atggtgaaac cctctttaat acgcaaaaac gagtgcaggg ttggtcggat tctcccttga
    15001 cagaacgtgg tatcgctcag gcccaagccg ttggtcagta ttttaaggag cagggaatcg
    15061 tctttaccag tgcctattct tccacgcaag agagggctac tgatacacta aaattagtta
    15121 cggatgctcc ctaccaacag ctcaaggggc tcaaggagat gaacttcggg atttttgaag
    15181 ccagagagga agaattgctt cccaagcacc gtccaggtgc tcgctctttc gaagacctct
    15241 tggttccatt tggaggcgag gatattcgag cagttggaag tcgtgtcaaa gaagcgattc
    15301 tctctgtcgc ggaaagcgat ccagctgatc caatcctcat ggtcagtcat ggtgcagcca
    15361 tttggggatt ggtgcaggtt ttggaggtca ccttcccacc aggcgtcttc ctgcctaact
    15421 gtgccatttg ccagctgact gttgaaaatg agcaaatacg atttgaaaaa ctgattgatt
    15481 ccgcacatga ttttaaggtt tatgatttta agtaaggagg tttagtatga actatcttta
    15541 cttgatgcgc catggacaaa ctcggtttaa tcaacaaggt cggattcaag gggcctgtga
    15601 ttctcccctg acagaactgg gaagagagca agcactagct gctcaccagt attttcaaga
    15661 gcaagggatt gagttcgata aaatctattc ttcaacccaa gaacgtgcct gtgatacggc
    15721 agaattggtc acagggagga cggattatat ccgcttgaaa gggttgaaag aacaggattt
    15781 cggagccttc gagggtcaac aggaatacct caatcctccc ttacagggag acattggcta
    15841 cggtgattat tttgtcacct ttggaggcga atcctatcaa gatgtccgac aacgaatggt
    15901 cgaaaccatt gggggcatca tggaagaagc agacaatcaa tctgttttag tagttagcca
    15961 tggagctgcc atagcccaat ttttcaggca ggtattgaca gattatccgc aagttcggat
    16021 gcgtaattgt gccatactga cttttactta tgaagacggt cactacgatt tgcaaagcat
    16081 tgtggatccc gttaataaag aaaatctcta tcaaaaagaa ggaaaggaaa aggagaaaaa
    16141 atgacagtaa caaaagtatt tccaaaagga tttttatggg gtggtgcaac agcagccaac
    16201 caatgtgaag gagcttacga tgtagacgga cgtggacttg ccaatgttga cgtagttcca
    16261 attggccaag accgagctgc cattatcact ggtcgtaaga agatgtttga ctttgaggac
    16321 ggttatttct atccagccaa ggagtctatt gatatgtatc atcacttcaa ggaggatatt
    16381 gctctctttg gtgaaatggg ctttaagacc tatcgtcttt caatcgcctg gtcccgtatt
    16441 ttccctaagg gagacgaatt ggagcctaat gaaaaaggcc tgcaatttta cgaggacttg
    16501 ttcaaggaat gtcacaagta tggtattgag ccattggtga ccatcaccca ctttgactgc
    16561 ccgatgcact tgattgagaa ctacggtggc tggcgaaatc gtaaaatgct tgaattttac
    16621 gaacgccttt gccgtaccct cttcacacgc tacaagggct tggtcaaata ctggctgacc
    16681 ttcaacgaaa tcaacatgat tctccacgca ccatttatgg gggcaggtct gtattttgaa
    16741 gaaggcgaaa atgtggaaca ggttaaatac caggctgccc accacgagct tgttgcctct
    16801 gccattgcta ctaaattagc ccatgaaatc gatccggaaa acaaggtcgg ttgtatgttg
    16861 gctgcaggtc aatactaccc acacacctgc cacccacgtg atgtatggga agctctgcaa
    16921 gaagaccgtg agaactattt ctttatcgat gttcaggcac gtggctatta tccaaactat
    16981 gccaagaaga agtgggagcg tgcaggtatc gagattgaaa tgaccaacca agacctagca
    17041 ctcctgaaag agcatacggt ggactttatt tccttctctt actactctag ccgtgtggct
    17101 tctggagatc ctgtagagaa ggaaaaaaca gcaggcaata tctttgcttc cattaaaaat
    17161 ccttacctag acgcatcaga atggggctgg cagattgacc cgctcggttt ccgcattacc
    17221 atcaattcca tatgggatcg ctatcaaaaa ccactctttg tggttgaaaa tggtctggga
    17281 gcagtcgaca cgccagatga aaacggctat gtggaagacg actaccgtat tgactatctc
    17341 cgccaacatg tcttggcaat gagagatgct attgtcgaag acggtgttga actcatgggc
    17401 tataccactt ggggctgtat tgacctagtg tctgccggaa cgggcgaaat gaaaaaacgc
    17461 tacggcttca tctacgttga ccgtgacaat gagggaaatg gcacactcaa acgctctaag
    17521 aaaaaatcat ttgaatggta taaaaaagtc atcgctacaa acggagcgga tgtgggataa
    17581 aacacgagag acaggtcttc ggacttgtct tttgttatcg tttccactat aataaaaatt
    17641 agttaacaat aaaggagttt tgcatgaaat acatcgtaaa caacagcaat gacccagctt
    17701 ataacatcgc cctcgaagcc tatgccttca aggaattaac agatattgac gaaattttta
    17761 ttctctggat caatgagcct gccattatta tcggtaagca tcagaatgct attgaagaaa
    17821 tcaacaagga attcacagac gaaaagggca tccatgtggt tcgccgtctg tcaggtggtg
    17881 gcgctgtcta tcatgacctc aataacctca actacaccat tatctcaaat aaggcagacg
    17941 agggagcctt tgacttcaaa accttctcca agcctgtcat tgataccctt gctacactgg
    18001 gtgtagaggc caatttcacc ggtcgtaatg accttgaaat cgacggaaag aaaatctgtg
    18061 gtaacgctca agcctatgcc aaaggtcgca tgatgcacca cggttgcttg ctctttgatg
    18121 tggatatgtc tgtattggca agtgccctca aggtcagcaa ggacaagatt gaatccaagg
    18181 gtgtcaagtc agtccgtgcc cgtgtgacca atatcaacaa cgaattgcct gagaagatga
    18241 ccgttctgga gttcaaggat gctatcctca accagatgaa gcaagaatac ccagacatgg
    18301 acgagtatgt cttgtcagaa ggtgacttgg ttcgtatcca agaaatccgt gatacccagt
    18361 ttgcgacatg ggattggacc tacggtcaaa caccagaata cactgtggag cgtagcgtgc
    18421 gttacccagc tggtaaaatc acaacctaca tcaaggctga aaaatcagtc atcgaatcta
    18481 tcaagattta cggagatttc ttcggaattg gtgatgtatc ggacatcgaa gacttattgg
    18541 tcggtactcg ctacgagtat gcggatgttc ttgccaaatt gcaagaaatc gacaccacgc
    18601 actatttctc acgcatgaca acagaagaag tagcaaaagc gattgtagct taatccatag
    18661 ggctgccctt tgtttgggca gtctttattt tttgtgaaaa tgttttcggc aaactgtttt
    18721 cacttgcaca ttttctatat tatggtataa ttattttctg tgagtatccc tcacttactc
    18781 gtggctagac catgagtcat tagaccaaaa ggaggaacat atcaatggct aaatacgaaa
    18841 ttctttatat tattcgtcca aacattgaag aagaagctaa aaacgctttg gtagcacgct
    18901 ttgactctat cttgactgac aacggtgcaa ctatcgttga atcaaaagca tgggaaaaac
    18961 gtcgccttgc atacgaaatc aaagatttcc gcgaaggttt gtaccacatc gttaacgttg
    19021 aagctaacaa cgatgaagct cttaaagagt ttgaccgttt gtcaaaaatc aacggcgaca
    19081 ttcttcgtca catgattgtc aaacttgacg cgtaagaagg tgttgtatga ttaataatgt
    19141 agtattggtt ggtcgtatga cccgtgatgc agaacttcgt tatactccgt ctaatcaagc
    19201 tgttgcgact tttactttgg cggttaaccg caattttaaa aatcaaaacg gtgagcgtga
    19261 agcggacttt atcaacgtag tcatttggcg tcaacaagct gagaatttgg cgaattgggc
    19321 taagaaaggt gctctgattg gtgttacagg tcgtatccag acacgtagct atgacaatca
    19381 gcaagggcaa cgtgtctacg ttactgaggt agttgcagaa agtttccaac tcttggaaag
    19441 ccgtactgcc cgtgaaggtc aaggtggagg ctattcagct ggcaactcgt ttgctggagg
    19501 aaatgattac aactcacctt atcaagcgcc tgcacaatct acaccaaact tcgctcgaga
    19561 agaaagtcca tttggagcaa gtaatccaat ggatatatca gacgatgacc taccattcta
    19621 ggtcaaaaat taataatata aaggagaaca cacatggctc aacaacgtcg tggcggtttc
    19681 aaacgccgta aaaaagttga ctatatcgca gctaacaaaa ttgaatatgt tgattacaaa
    19741 gatactgagc ttcttagccg tttcatttct gaacgtggaa aaatccttcc acgccgtgta
    19801 actggaactt cagctaaaaa ccaacgtaaa gtaacaacag ctatcaaacg tgctcgcgtt
    19861 atggctcttt taccattcgt aaacgaagat taaaaaaatg gtccagtgga ccattttttc
    19921 atgagctcga aattaagaaa gcgaattaag tccttgaatg gactgttttt tcatgagcat
    19981 gaaaataaga aagcgaattg agaccccgac gggtcttttt agcttgcgcc caagaaactg
    20041 gaaaggcgca acggcgagag tggaccattt tttcatgaac tcgaaattaa gaaagcgaat
    20101 taagcccttg aatggactgt ttcaacccga gccaagaagt tagaaagcga ggcacagtct
    20161 ggggatagac tgtttcagca gattacatag acaagtgaca gcggataaag ttctcgggcg
    20221 atttgatagc tggactgtat agataaacaa aagcgactgg aatattccaa tcgctttgtt
    20281 ttattattga actggtgaca tggcattgcg aatgttatat ttctttttca agaggtagcg
    20341 tgcaccgaaa gcagcagctc cgataagaat agtaacaagc ggagggagaa ctgggttaaa
    20401 agcggccgga atcagtgctg ccagaatgaa aacgactgac caagccagcg tagcaagtcc
    20461 aagaaaagcg atagctttca gcaatttagg acgttgccca gttttgattt gttctctgta
    20521 aacgaaatgg tacatcaagt acatccctgc accaacaccg aaaccgatga caaataaggt
    20581 taatagccca tactgtgcac cttgaccaaa aagattcatc aaaccattga taccagcaat
    20641 aattgcaagc atgaaaagac tagaatctag ccacatcaac catggatttt cattgtattc
    20701 cacttggtct ttcttttcct gttcagaaac tgtcttacta gcagcccact cacttggtgc
    20761 tccatagaga ttgcgggcag tcacgccagt tttttgcttt tcaatgatag ttggtaaaat
    20821 ttcttccaag agttctttga tttcgctatc agactttcca tctttaatca gttggttggt
    20881 cgcaatatgg ataaattctt ggttcttttt tgttaatccg ttgatttctg taaatgacat
    20941 gttaatctcc ttaggttaaa accatctctt acggatgaag tagaaggtga gtgataggct
    21001 gattgcaaat gcgaagagga taatccacca aaaagcatag ggaatatcgt taaaaggaat
    21061 gatgttgtcc ttaaagttca taccgtatgc agagaagacc atggttggga tagagagaac
    21121 gattgtcact aatgccaagg ttttcatgat ggtattctgg ttgttagaaa tgatagaagc
    21181 aaaggcatgg gtcatagagt tcaagatgga accatagatg tctgccatct caatggcctg
    21241 ttgggtttcg accaaggtat cttcaagcaa atcctcatcc tcttcatact tacggagtag
    21301 acgtgaggaa ctagcaagtt ttttgaccaa acgctcattg gttttgaggg aagccttgaa
    21361 gtagacgatg gttttttcca attccatgag tacaattaat tcttcatttc gagtggcctc
    21421 atggagttgc ttctcaatct cttcgctctt tctgtcaatg gtccgtaggg ctgacaagta
    21481 gagttgagca ttgcggtaga gaatttggaa aacgaaacga gacttcatga aagtgaagaa
    21541 attccgtaga cgacgatgga taaactgctc aaacagtggc aatggttcca aacaagttgt
    21601 gataatcgca tcatctgtta gaataattcc caaaggaata gtaatataat atgtcttgtt
    21661 gtttcgttct tcaagaattg ggacgtcgac caggatgagc gtatagtcat cttcgacggt
    21721 gatacgagag gattcttccg catcgagcgg tgctcgcagg tcagcgatgt caattttata
    21781 gtgctcagcc acttccatag actcgctctg ggaggggttg accaaattaa tccaagcccc
    21841 tggttcaaag gtttggattt cttctaaatc agtcatggtt gatagaaaaa tttgtttcat
    21901 atctttgccc tccttttatc tttgttgagt taacgcaacc tctattatac caaaaaatgt
    21961 gcagtttcgg aaaggtttta tatagaaatg tgatataata tgtagacatt taagcgaata
    22021 agttgtacgg acttttcctt gttccgtata ccatagctta tagcctctta agaaaggaat
    22081 tgaacacggg ctaaatgcgg tgtcaaaaat tgctggtttc ttcatgtttc agcatggaaa
    22141 ctggcttttg cgtcgtagac atctgtgctt tcttcggtta tcaacctgtg aaagcactag
    22201 ttgtcttgac gggggtgcgt cttcggaatc acagatttcg gacttaccgc ctattttgac
    22261 ggtgcgtttt acggtctttg tatctttaga aaggaaatga acacgggcta aatgcagtgt
    22321 caaaaattgc tggtttcttc atgtatgagt atggggaatt agcttctgcg tcgtagacat
    22381 ctgtgctttc ttcggttttc gacctgtgaa agcactagtt gtcttgacgg gggtgcgtct
    22441 tcgaaatcac agatttcgga cttaccgcct attttgacta tgcatttgac gccctttgta
    22501 tcttagaaag gattcgaatg cgtgctaaaa gcgtcgagaa aaagacaaaa tgttttgtca
    22561 ctgatgtgac gtcacatttt cctattttct tctcgctttc ttaacgcact tatatctaga
    22621 aaggaattga acacgcccta aacgctgtgt gaaaaagaca aattctccta gagcgttaac
    22681 gctcttcgtc aaatttccta ttttcacctt gcgttttacg ggctctgtat cttaaattat
    22741 gcaagaaaat attgtgattc atggggcgcg tgcccataat ttgaaaaata ttgatgtgac
    22801 cattccgcgt gagaagctgg tggtggtgac tggtttgtcg gggtctggta agtcgagttt
    22861 ggcttttgat actctgtatg ccgaaggcca acgtcgctat gtggagtctt tgtctgccta
    22921 tgctcgtcag ttcttgggca acatggacaa gccagatgtg gattccatag aggggctcag
    22981 ccctgctatt tccattgacc aaaaaacgac gtcgcggaat ccgcgttcga cagtcggtac
    23041 agcgacggaa atcaatgact atctccgcct gctctatgct cgggtcgggg tgccgtactg
    23101 tatcaatggg cacggggcga ttgcggcttc ttcggtggag cagatcgtcg atgaggtctt
    23161 ggaattgcca gaacgccagc gtttgcagat tctagctccg attgttcgta agaaaaaggg
    23221 gcaacacaag accatttttg agaaggtgca gaaggacggc tatgttcggg ttcgtgttaa
    23281 tggtgatgtc tatgatgtgt cggaagtacc agaattgtcc aagagcaagg ctcataatat
    23341 tgaagtcgtg gtcgatcgga ttgtgatcaa agagggcatt cgctcgcgac tttttgattc
    23401 tatcgaagcg gccctgcgga ttgcggacgg ttatgtcatc attgatacca tggatgacaa
    23461 ggagctgctt ttctctgagt actatgcctg tccagtttgc ggctttacag tgcctgagtt
    23521 ggagcctcgc ctcttctctt tcaatgctcc ttttgggtct tgtagcgact gtgacggttt
    23581 gggcatgaag ctggaagtgg atacggattt gattgtccca gatgccagca agaccttgcg
    23641 tgacggggct ttggctccgt ggaatcctat ttcttctaac tactaccctc agatgttgga
    23701 gcaggccatg aaccattttg gtgtggacat ggacaagcct tttgaggaat tgacggaaga
    23761 ggagaagaac ctgattttca acggttctga cgggaaagaa tttcatttcc attatgaaaa
    23821 tgagttcggt ggggtgcgtg atatcgatat tccctttgaa ggtttgatta ccaatatcaa
    23881 ccgtcgctat cgtgaaacca atagtgatta cacacggaca gtcatgaaga cttacatgaa
    23941 tgaattgacc tgtggaacct gccacggcta tcgcctcaat gaccaagccc tgtctgtcaa
    24001 agttggtggc gagcagggac ttcatatcgg gcagttgtct gatttgtccg tagcggatca
    24061 tctggaagtc attgaacatc tgaccttgtc tgaaaatgaa gccaccattg caacacccat
    24121 tgtcaaggaa atcaaggacc gcctttcctt cctaaataac gttggtctca actatctgac
    24181 tctttcacga gcagcaggta ccctttctgg tggtgagagc cagcggattc gtctggcgac
    24241 tcagattggc tctaacctgt ctggtgtcct ctatattctg gatgaaccgt cgattggtct
    24301 gcaccagcgg gacaatgatc gtctcattgc cagcttgaaa aagatgcgtg atttaggcaa
    24361 taccttgatt gtggtggagc atgatgagga caccatgcgt gaggcggact ggttgattga
    24421 catcggtcca ggtgcgggtg tctttggtgg agaaatcgtg gcttctggaa cgcctgccca
    24481 agtagctaaa aataaaaaat ccattacagg ccagtacctg tctggcaaac gtgaaattcc
    24541 agttcctcta gaacgtcgtg ttggcaatgg tcgtttcctg gaggtaacag gtgccaagga
    24601 aaataacctg caagatgtga ctgttcgttt cccgctcgga aaatttgtag cggtgacagg
    24661 ggtgtctggc tcaggcaaat caaccctggt caattccatt ttgaaaaaag ctattgctca
    24721 gaaactcaat cgcaattcgg acaagccagg caagttcaag tccatttcag gcatcgaaca
    24781 cttggaccgc ctgattgaca ttgatcaaag cccgattgga cgaacaccgc gttccaaccc
    24841 agctacttat acaggtgttt ttgacgatat tcgggacctc tttgctcaga ctaatgaggc
    24901 taaaatccgt ggctacaaga agggacggtt ctcctttaac gtcaaaggtg gtcgttgtga
    24961 ggcttgttct ggtgatggta tcatcaagat tgaaatgcac ttcctgccag atgtctttgt
    25021 accttgtgaa gtctgtcatg gacaccgcta caattctgaa accttggaag tacattacaa
    25081 ggagaaaaat atcgcccagg tccttgatat gacggtcaat gatgcggtcg agttcttcaa
    25141 acatattccg aaaattgagc ggaaacttcg caccattcaa gatgtaggct tgggctatgt
    25201 tacccttgga caacctgcga caaccctatc aggtggggag gcccagcgga tgaaattggc
    25261 atcggaactt cacaagcggt cgactggtaa gtccctctat atcttggatg agccaacgac
    25321 aggtctacat actgaggata ttgcccaatt gctcaaggtc ttggcccgct ttgttgatga
    25381 cggcaatact gtccttgtta tcgagcacaa tctggatgtc atcaagacag ccgaccacat
    25441 tatcgacatg ggaccagaag gcggtgtcgg tggtggtacc gttgttgcaa caggaacgcc
    25501 agaagaagta gctagcaacc cagccagctt tacaggccag tatctgaaaa tgaagttgaa
    25561 ataaaaagcc gcctctgcgg ctttttttct ctatctgcta ggaagaattg gtatttgtct
    25621 gtatttctgc tataatagtt acaaaatctt tggaggtagc ttatgcaagc gcgtgtggaa
    25681 aaatttgagg caaaattagc ccaatctgat gtggatggaa tcttggtgac tggtcaaaac
    25741 aatatttact acctgacagg tttttggggg acagaggcga ctgtcttcat cagtggcaag
    25801 cgtcgcttgt ttgtgactga ttcgcgttat accttgatag ccaaggcatc agtaaaaggt
    25861 tttgatatta tcgagagccg ttttgccctt gaagagattg caaaggtgat taaggaagat
    25921 gggcttgaaa aaattggttt tgatagcgag gtgacctatg gtttctatca aagtctgacc
    25981 agcatttttg agggttatca actggttgct atgtcaaact ttatcgaaga cttgcgcatg
    26041 attaaagatg agaaagaaat tgcgaccatt cgccgtgcat gtcaaatttc ggatcaggct
    26101 tttattgatg ttcttgactt tatcaaacca ggtcagacga ctgagatgga cgtcaatcat
    26161 ttccttgacc accgcatgcg tcaactcggt gcagaagggg catcatttga gttcatcgtg
    26221 gcatctggct accgctctgc tatgcctcat ggaagagcgt ctgaaaaggt tatccaagct
    26281 ggtgagacct tgacgctgga ttttggttgc tattaccagc attatgtcag cgatatgaca
    26341 cggaccatcc atatcggtca tgtgacagat caagagcgtg agatttacga tgtggtcttg
    26401 cgtgccaata aggctttgat tgagcaggcc aaggaaggtg tcacctaccg cgagtttgac
    26461 gctattcctc gtgagattat caatgcggcg ggttacggag ccaactttac acacggcatc
    26521 ggtcacggta tcggcttgga tattcatgaa tatccgtatt ttggaaaatc tgacgagact
    26581 atcaaggcag gtatggtctt gacagatgag cctggtatct atttggatga caaatatggt
    26641 gtccgtatcg aagatgacct attgattacg gaaaacggtt gtgaagtctt gacattagct
    26701 cccaaggaat tgattgtcat ttgagaaatt tgacagtttg tggtagaata aagcgaataa
    26761 ataaaatttg aagaggtatt caatacaatg attgaagcaa gtaaacttag agcaggtatg
    26821 accttcgaag ctgaaggtaa attgattaaa gttttggatg caagccacca caaaccaggt
    26881 aaaggaaaca ccatcatgcg tatgaaattg cgtgatgtac gtacaggttc aaccttcgat
    26941 acaacttatc gtccagatga aaaatttgaa caagctatta tcgaaacagt tccagcacag
    27001 tacctttacc aaatggatga cactgcttat ttcatgaata ctgagactta cgatcagtat
    27061 gaaattccag tagcgactat caaagaagaa ttgctctata tcttggagaa ttccgatgtg
    27121 aagatccagt tctacggaac agaagtgatc ggtgttactg taccaacaac tgttgaattg
    27181 gtcgttgcag aaacacaacc atctatcaaa ggtgcgactg ttacaggttc aggtaaacca
    27241 gctacgatgg aaacaggtct tgtagtcaac gtaccagact ttatcgaagc aggtcaaaaa
    27301 ctcgttatca acacagctga aggaacttac gtatcacgtg cttaatcgtt tcctcttaag
    27361 aaaggagagc ctatgacaac agaatatcaa ggcgagattg ttatcgcacc tcgcgtcctt
    27421 gaagtaatta caggtattgc tgcagcgaaa gttgatggag tgcattccct tcaaaataag
    27481 cgtgtagcag acagtttgtc aaaaacatcg ttaaataaag gtgtttatct tgaaactgac
    27541 gaggaaggtc gagtgactgc ggatatttac gtctatctag aatatggtgt aaatgttcct
    27601 gccgtatcaa tggacatcca acgtgcagtc aagacagcag tttataacta tgcagaagtt
    27661 caagtggctg cggttaatat ccatgtcagc ggcattgttc ctgataaaac accaaaacca
    27721 gcattgaaag atttattcgg agaggatttc cttgatgaag aataatagac atgccctgcg
    27781 taagtgtgct ttgcaggcaa tcgtatcact tgaatttggt caagaacctg ttcaggcagc
    27841 tcagttttct tatctttatg atagagagga agagcaagaa ggagttgaaa ttccgctctt
    27901 tctccttaac cttgtcaatg gtgtagcaga ctatcgtgat gacttggata aggagttatc
    27961 tagccgatta aaagcaggat ggaccttaga ccgtttaacc ttgattgaca agaatatcat
    28021 gcgactaggc ctatttgaaa tcttacattt cgaagaaacg ccagaccgcg ttgctgttaa
    28081 cgaggctatt gaattggcta aagaattttc tgatgaatct tcagctaagt ttgtcaatgg
    28141 agttttgagc cagtttatta aagaagaagc ctgatgagct tcttttttac ttgagaaaca
    28201 agagaatgta tcacaaaaaa actgcaccta atttttatta ggtacagttt ttttatatac
    28261 tagctcctgc tagtaggcta atcggtagga tatagtctgt ttttgtcagt ttttttcctt
    28321 cgattttatc aactaatagt tcaaccagca ggcgagctat ttcggttaat ggttgcttga
    28381 tggttgtcag ttgaggatag tagttttcga tgaaactggt tccatcataa ccaatcaatt
    28441 taatatcttc tggaatagac agtttcattg aacgaatgac gttccatacc agcatagccg
    28501 ttgcatcgcc agatacaaag ataccatctg gtttttgctt ttcaagaatg gctcggattt
    28561 ccatctcctt gcgcacaggt gagaaatcac tggagatatg aaaaacttcg gctttgttca
    28621 aaatagatgt gaaacctact tggcgaaggc cggtaggtga attggaatca tcgttccccg
    28681 taatcattaa tggattgtga catcccgctt tttgtaaggt ttgggcagct aataccccgc
    28741 cagcaaaatt atcggaagag acaatgggga tggaaggaga gaggttgcgg tcaaaagcga
    28801 taattggagc tacgacacgg tcgtagtctt ggataccgag attgtgactg cccgagataa
    28861 tcccatctac ttggttagct gctaacattt ctagatactc acgttcctta tcggactcgt
    28921 gctggctatt gcaaataatg gttttatagc ctcgatcaaa aagctctttt tctaaatggc
    28981 taatcaattc agaataaaaa atattgttta ttgttgggaa gatgagacca actaattttg
    29041 cagatttacc ttgcaaacta cgagctaaat tattaggttt gtagcccagt tcacgcatag
    29101 cctcttcgac attgcgaatg gttttttcag ataggtatcc ttttttattg ataacgcgcg
    29161 agacagttgt tgggctgacc ccagctaact gtgcgacatc agttagtttt gcgaccataa
    29221 tctaaatggt agtaggttcc ggtcggatta ccagtcgtaa tgacgatacc agactgatcc
    29281 gcatcaggga agacacgacc agaaaatact ttctctcctt tattgataaa gatttcaaag
    29341 acagatttgt cgatgaagat attggctgtg agagctcctg gagaaatctc gcaagagcga
    29401 cttgtaccga aatcgagtgc gaatggctga ccacagttgc cgcggtccaa tgtaatctgg
    29461 ccttgttcag tgtccacaga caagaccaag cctttttctg cttcatttgc gaacaatacg
    29521 atttcgtgct tcgtgcccgc tgaaaaatca agttccaatt cgtaaacatt attagtttct
    29581 ttttgggctg aaaatggctg agcttcttta cgaagactgg tgatcgcatc aactggatac
    29641 tggtagagct taccgtcctt gatggttaat tctttaacca atgacagaca gccttggtag
    29701 tcataagcat cggttgggta ttccacatct ggaagtgcca accagctaac agatagagtt
    29761 cgaccgtctg gtgcattaaa ggcctgggtt gcatagcact caaaaccata gtccagattg
    29821 atcaattcgc tcggttcgac aatgcttgct gtttcaggat cgaaactctg accaatcttg
    29881 tacatatttg gatagatatt gctatagtcc aatacctctt tgtccaagcc ttgtggacag
    29941 tagagaagga tgggttgctc accgacgaac gcaatatttg ggcattccat catataagcg
    30001 gtcatatcat tggcaaaatc aaggtcacca acataggacc agttgaggta gtcgttattt
    30061 tcagctttat aaagtttgac aattcccttt ttatccagat tttgtccacc gacaatagcg
    30121 taatattgcc ctttatagtt gaaaatttgt ggatcacgga aatggtccgt ggtatcttct
    30181 ggttgctcaa tcaagacttt atcaatcttt tcaatcttac catctttatc catcaaggca
    30241 ccgatttggt atggatggcg aacccaattt tcatcgcgga catttccagt atagaagagg
    30301 aagagcttgt cgccaaattc catggcagat ccagagtagg caccgtggct atcaagtggt
    30361 gtatctgggt acataaccgt accagttttt tcaaaatgga ccaaatccgt tgattctgta
    30421 tggacccaag attttaatcc gtgcgcagca ccgaatggga agtattggta aaaaagagtc
    30481 cactttccat taaaataaga aaatccattt ggatcattta acagtccatg tggtgtttca
    30541 atatggaaat ttgtcctcca aggagaattc tctgtattct tctttatttt ttcaatatat
    30601 tcgggtgtcc agtcagcata agctctgtaa cgctcctctg tcgtaaatgc cataattttc
    30661 tccaaatttt ctagtttttc attaatatag taaacgtttt cttgcaaaaa atcaagtttt
    30721 aacaatgttt tttgataaaa tttaaataaa atgtgataaa cgtttgacat attttgtaaa
    30781 agtgatataa taaaaccatc gaaagtttga aaacactttc aaaaatatac aaaaaggaga
    30841 tttttgtaaa tggattataa aaaagtttct gccgaagtga tcgaagcggt tgggaaagat
    30901 aatctagttg cggcagcaca ctgtgctact cgtcttcgtt tggttctgaa ggatgaatcc
    30961 aaagtgaacc aaaaagccct cgatgataat gcagatgtca aagggacatt cagtacgaat
    31021 ggtcagtatc aagttatcat cggacctggt gatgtaaact ttgtatatgc agagctcatc
    31081 aaggctacag gcttaaaaga agtttcaaca gatgatttga aacagattgc agcgcaagat
    31141 aagaagttca atccattgat ggacttgatc aagctcttgt cagatatctt tgtgccaatc
    31201 attccggctc tcgttgctgg tggtctattg atggcgcttc gtaacttctt gacttcccca
    31261 gatttatttg gtccaaaatc aattgaggaa atgtatcctg ctatccaagg catttctgcg
    31321 atgattcaat tgatgtcagc agctccattc atgttcttgc cagtccttgt aggtatctcc
    31381 gctgcgaaac gctttggtgc aaaccaattc ctcggtgcgg ctattggtat gatcatgacc
    31441 actccagatt tgggtggtaa agaagctttt tgggatattt ttggtttcca agtagctcaa
    31501 acaaactatg catatcaggt tattccggtt cttgcagcag tatggcttct tgcgacgctt
    31561 gaaaaattct tccacaagaa gttgccatca gcagttgact ttacattcac accattattg
    31621 tcagtaatga ttacaggttt cttgacattt acagtcatcg gtcctgtgat gttaaccgta
    31681 tctgatgcga ttactaacgc aattgtttgg ttgtacgata caactggtgc gttgggtatg
    31741 agtatctttg gtggtactta ctcattgatc gtaatgactg gtcttcacca atcattccca
    31801 gccatcgaga cacaactttt gtctgcttac aacaataatg gtacaggata cggagactac
    31861 atctttgtag ttgcttcaat ggctaacgtt gcacaaggtg ctgcaactct tgctatctat
    31921 ttcttgacaa aaaatgcaaa aacaaaaggt ttgtccggtt cagcagcagt ttctgccttc
    31981 cttggaatta cagaaccggc cctcttcggg gttaacttga agtacagatt cccattcttt
    32041 tgtgctcttg caggttcggc tatcggtgcc ctctttgcag gtttgactca tgttatcgct
    32101 gtatctcttg gggcagcagg tttcattgga ttcctctcaa tcaaagcatc ttctatccca
    32161 atgtatgtag ttgctgaggt gattgctttt gctgctgcct ttgcctttac ttacgtttat
    32221 ggcaagacaa aagcatcagc agttttcgct gatgaagcag caacagcagc tgcggcagca
    32281 gtgacagcgt ctgtagttga aacttcagaa gaagttgaag aaactgcaga aatctttact
    32341 gcacaagaaa ctcttgtatc tccactttca ggtgacgttg ttgcccttga aaatgttaat
    32401 gacccagtct tctcatcagg agctatgggt aaaggtttgg ctgttaaacc gtctgagggt
    32461 gttgtttacg ctccagcaga tgcagaagta acaattgctt ttgaaacagg ccatgcttac
    32521 ggattgaaaa cagcgtctgg tgctgaactt ttaattcaca tcggtattga tactgtatca
    32581 atgaacggta atggatttga aaaattggtg gcagcaggtg ataaagtaaa agctggtact
    32641 ccgattgcta aatttgatgc aactaagatt gcagaagcag gtcttgatga tacaaccatg
    32701 atcatcgtga ccaacacagc tgactttgca gaagtatctc cacttgcaga gggaacaatc
    32761 gctcacggtg tagaattctt gaaagtagct aagtaatctt atataaaatc gatttgtaga
    32821 gtctgcctct actacataat taacaggaaa aaggctttca gcctttttct tctgtttgct
    32881 tatatgttat aataaaagtg ataaaaattt tggagacaat caaatgacaa aattatatgg
    32941 tagtttagaa gctggtggta caaagtttgt ttgtgcagta ggtgatgaga actttcaagt
    33001 tgttgaaaaa acgcaatttc caacgacaac tccttatgaa acgattgaga gaacagttga
    33061 attcttcaag cgctatgaag atcgtttggc aggtattgcg ataggttctt tcggtccaat
    33121 tgatattgat caaaactcac agacttatgg ttacatcact tcgacaccaa aaccacactg
    33181 gtcaaatatt gatttacttg gtctgattgc taaggaattt aacattccat tctacttcac
    33241 gacagatgtg aattcatcag cttttggtga aaccctggtt cgtaaagggg taaagagctt
    33301 ggtttactat acaatcggta caggtattgg tgcaggtgct attcagaatg gtgagtttat
    33361 cggtggcttg ggtcataccg aagcgggtca tacttacgtt gccctacacc cttacgatgt
    33421 aaaacatgaa tttaaaggga tgtgtccatt ccacaatggg tgtttggaag gcttagctgc
    33481 aggtccttct cttgaaggcc gtacaggtat tcgtggtgaa ttaatcgagt taaattcaga
    33541 cgtttgggat gtgcaggctt actacattgc tcaagcagca gtacaggcaa ccttgcttta
    33601 tcgtccgcaa gtcattgtat ttggtggagg agttatggct caagagcaca tgcttaatcg
    33661 tgtacgtgag aagtttgtcg gtttgatgaa cgattatctc ccaacaccag atgtcaaaga
    33721 atatatcgta acgccagctg ttgctgaaaa tggttctgca actcttggga attttgcttt
    33781 ggcaaaacag gtgtcaagaa attcataatt gtttatgtga acagattcat ttgtactttg
    33841 gcgatagata tattgaagaa gaatattggg tgaagttgtt ttattatcac aataacttga
    33901 cagtgaggtg agagttttta tgtaactctc acctcttttt acctaaaaaa ctgtattcat
    33961 aagagatagc cgtttgttaa tcaaggcagt ctttgggagt aggaaagaat cagaccggct
    34021 taaaaagagt tcgtcaacaa ctctttaatt taagtttttg agccgaaaca gcgcctcaaa
    34081 aaactaacga tgactataaa agattagagt ttgccattgt tggagttcat cgggtatgaa
    34141 aaagagaaac gagttccaca acttttgatg attattcata agcaaggttg aaaatctttt
    34201 tattggcgat agtgtagtga tattttttaa ccagaggaaa gttgttgttt ctcaattgta
    34261 tgaaaacgct atatctaaag taatgatgcg gagacactaa agtctataaa aacaggaaaa
    34321 gcacagatag atagcagaaa aaagatagtt aaaagtgtta caattgggat gcaaatttta
    34381 ggctgggaaa ttattcttag aagtatttat atggaggact tatgattcca attcaacagc
    34441 atggtaagcg ctggcttatt ttgacactta ccctttctac ttcagttctt gcgcttgcat
    34501 ctatcggagc atcacaaccg gttgtttttg cagatgaaaa ctcacattta caatcacaaa
    34561 aaaacattaa taaaatagag gttctggact ggaaaacatt ttctaaaaaa ttgagcgaat
    34621 actcctcgga ccaacgacaa tttcatgttt tgaaattggg atttgaaaat cgtcttggga
    34681 cattatcaac ttgggatgaa ttggaagaat ttggtaaaaa caacaatttt ttggtaatca
    34741 atggtaaagt tactcaaaat attcatgact ttccacatat ccttgttatg aacaagggtg
    34801 atgttattgc tcataatgaa gaagattatc ataatcagat gagagagttg aggttttcgg
    34861 gaaatggcga cttacataat agtatggagc caaaacggat tcatgcacta tttaagattg
    34921 aacttgactc caataaacgg cagttattaa atgctgcggg tctaggaact gctgagaata
    34981 gtttgaaaaa tattaatgga atgactattt actcgcatgg tcttacggtt gataataagt
    35041 actatgagga ttatagtaaa tatactcata attcagttaa aaatataaac gttaccaaag
    35101 agcgttttat agcaaatgat gacttgattc ataagttgat agagtcctca gaagctatga
    35161 agcaatcaag tgaacgcgat aaagttaagg catttgttca gtatgttgcc aatcatacaa
    35221 catatgattg ggaagctgct aataaggctg ttcaaaatta tgcagacatc aattactact
    35281 taggatcaga tttgttcgcg gttactgaac gtcagaaagc gatgtgtgta ggtttcagca
    35341 ctactgcggc ccgtgcattt aatatgttag ggcttcctgc ttatgttgtt gttgggaaaa
    35401 atgcagaagg agtcccgcat gctacagcgc gtgtttatta tgataaaaag tggcacacca
    35461 ttgacggtac aggttttatt acagatatta ctgattctaa taagcatcag cgctcagcta
    35521 aatatagtga aaaacacttt t
//
LOCUS       DASFSW010000006        35398 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.006, whole genome shotgun sequence.
ACCESSION   DASFSW010000006 DASFSW010000000
VERSION     DASFSW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.006"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(98..667)
                     /locus_tag="U1099_000249"
     CDS             complement(98..667)
                     /locus_tag="U1099_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEM4866600.1"
                     /translation="MELPMTQAFFPIHTLETVSPELRENLATVKKNNGGYIPNLIGLL
                     ANSPTALETYQTVSGINRRSSLNPTEREVVQITAAVANGCGFCVAGHTAISIKQVKMP
                     DVILQALRQGTPIETDAKLDALARFTLAVIHEKGKVEQPLLEEFFQEGYTAENALDVV
                     LGVSLATLCNYANNLINTPINPELQAYAL"
     gene            complement(821..1516)
                     /locus_tag="U1099_000250"
     CDS             complement(821..1516)
                     /locus_tag="U1099_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4866601.1"
                     /translation="MKALIFDVDDTLYDQIQPFERALERHIEVAREQIEPLYLSFRRY
                     ADEVFEATAIGKMSLKDSHIYRMKHALADFGYQVSDATALAIQIDYDYFQGQIELSPV
                     FPEIFSWCQAQGIAMGIITNGPYRHQLRKIRTMGLVNWFELEHVLISGQVGITKPNPA
                     IFQLMEERLGMSGEDICYLGDSFENDVVGAKAANWKAIWFNHRKRVEPTAPYQADKVV
                     TDWDELVEVIQSF"
     gene            complement(1570..3309)
                     /locus_tag="U1099_000251"
     CDS             complement(1570..3309)
                     /locus_tag="U1099_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866602.1"
                     /translation="MTILGFLLKQIRRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     YGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
     gene            complement(3309..5036)
                     /locus_tag="U1099_000252"
     CDS             complement(3309..5036)
                     /locus_tag="U1099_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866603.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDKAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKATQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGQGNFFI
                     NHQSVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEEGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            complement(5605..5677)
                     /locus_tag="U1099_000253"
     tRNA            complement(5605..5677)
                     /locus_tag="U1099_000253"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5642..5644),aa:Thr,seq:ggt)
     gene            5834..6304
                     /locus_tag="U1099_000254"
     CDS             5834..6304
                     /locus_tag="U1099_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEM4866604.1"
                     /translation="MKLQNLQVGQSYLVKDCLTQDDIRKHLAHLGLKVGEEIRIISKT
                     KTNAIFQVKASRLALDREIIESLVLTEKSATEIINLSEAPIGSSAKVMDIYATGALRR
                     RLMDMGLTKNTQLFLKKVAPLGDPIEITLRGYELTLRKSEAQMIGVQITSEVRK"
     gene            6301..8445
                     /gene="feoB"
                     /locus_tag="U1099_000255"
     CDS             6301..8445
                     /gene="feoB"
                     /locus_tag="U1099_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEM4866605.1"
                     /translation="MTKHIALAGNPNSGKTTLFNLLTGTNQRVGNWPGVTVERKSGTI
                     KKRKHLLIQDLPGIYSLSPYTPEERVARDYLIADQPAAILNVLDATNLERNLYLTLQL
                     LEMGLPIAIALNMTDALKSQGRSINSDQLSYQLGVPVQPISALKKLGISQLLHEVEKI
                     TNQQAEPIYPQYDKQFEAGLAQVIDLLSDSIPNHQKRFYAIKLLEQDQVILDQLQLNA
                     QDMLDLTEIIAILEKIYADDMEAIIVNQRYQFIEKITELVTKDSDKAFNLSDNIDRLV
                     TNRFLALPIFAAVMWLTYFLSIQTVGTMGTDWVNDVLFGELVPGLIQANLDRFEIAGW
                     LQSLVLDGIIAGCGAILGFVPQIFVLFVCLGILEDIGYMSRVAFVMDRIFRRFGLSGK
                     SFIPMLISTGCGVPGVMASRTIENEQDRKITIMTATFMPCSAKLPIISLVAGAFFPDN
                     PWIAPSAYFVGMAAIVLSGMALKKTKQLGGVASPFIMELPSYHLPKLSTVLRYAFDKA
                     LSFIKRAGTIIFVTNIIIWFSSSYNWSLQMVETDESILASIGHSFSLLFAPLGFENWR
                     ATVAAITGLLAKETVIATFGILYKLGETTEENPELWGLLQQDYTALSAYSFLVFNLLC
                     APCFAAIGAIHREMGEAKWTWIAIGFQTGLAYASSLVIYQIGLVLIHGQLPTVWTFIA
                     IFLIITAIYSLVKKPTNTLPIVTLKTLEKGEY"
     gene            8445..8597
                     /locus_tag="U1099_000256"
     CDS             8445..8597
                     /locus_tag="U1099_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEM4866606.1"
                     /translation="MPTLILFLIIVILFSLAIRSLIKGKGSCGDCSCDCTIKQEMNES
                     SHHRHP"
     gene            complement(8650..9579)
                     /locus_tag="U1099_000257"
     CDS             complement(8650..9579)
                     /locus_tag="U1099_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4866607.1"
                     /translation="MSNRFFRHFIESLKSLKRNGWMTIAAISSVAITLTLVGLFASVI
                     LNTAKLASDLEQNVRINVYLRANSTDQAETIVNEAGKTVANPDYKKVYNQITALENVQ
                     SVTYSSKDEQLQKLTATLGDTWNLFQGDANPLYDAYIIDTTEPQYVKTVAAEIAKIDG
                     VTEVRDGEVETERIFKLANLVRTWGLAATGLLLFTAVFLISNTIRITIISRSREIQIM
                     RLVGAKNSYIRGPFLWEGAWVGLLGAILPSALVYSFYKMIYTSVNASLASQDLSLISM
                     DVFVPGMIGALFVIGIIIGSLGSVISMNRYLKI"
     gene            complement(9572..10264)
                     /gene="ftsE"
                     /locus_tag="U1099_000258"
     CDS             complement(9572..10264)
                     /gene="ftsE"
                     /locus_tag="U1099_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEM4866608.1"
                     /translation="MGIIEMKDVSKKYGNGTTALRGVSVNVEAGEFAYIVGPSGAGKS
                     TFIKLLYREEKLDKGSLKVGKFDLAKIKKRDVPLLRRSVGVVFQDYKLLPKKTVFENI
                     AYAMEVIGEKPRNIKKRVMEVLDLVGLKHKIRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPENSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIEDGRVV
                     RDEAEGEYGYDE"
     gene            complement(10407..11502)
                     /gene="prfB"
                     /locus_tag="U1099_000259"
     CDS             complement(join(10407..11429,11431..11502))
                     /gene="prfB"
                     /locus_tag="U1099_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775232.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEM4866609.1"
                     /translation="MDTAEIRQKIEELGKKLTSFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNLAAQKTSQELNELKNTYGNFHQMLDLYDESEILLDFLAEDESVRDELVEKLVE
                     LDKTMTAYEMTLLLSDPYDHNNAILEIHPGSGGTEAQDWGEMLLRMYQRYGNAKGFIV
                     ETLDYQAGDEAGIKSVTLSFTGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEIEIRDDEVKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVTQSTVDRT
                     QYGNRDRAMKLLQAKLYQLEQEKKAAEVDSLKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTGYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(11537..12664)
                     /gene="queG"
                     /locus_tag="U1099_000260"
     CDS             complement(11537..12664)
                     /gene="queG"
                     /locus_tag="U1099_000260"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEM4866610.1"
                     /translation="MNIKEEIIKLSQEIGISKIGFTTADDFAYLEKSLRASQEEGRSS
                     GFEHKNIEERIRPKLSLESAKTIISIAVAYPRHLPVKPQKTQYKRGKITPSSWGLDYH
                     YILQDKMERLARGIERLTDGLEYKAMVDTGALVDTAVARRAGIGFIGKNGLVISKEFG
                     SYMFLGELVTNLDIEPDQPVDYDCGDCNRCVAACPTSCLLGDTTMNARRCLSFQTQDK
                     GMMDLEFRKKIKTVIYGCDICQICCPYNKGISSPPVVEIDPDLAEPELIPFLDLSNGQ
                     FKEKFGMIAGSWRGKNILQRNAIIALANSNDRSAIPKLLEIIDKKQNPVHMATAIWAL
                     GQLVKQPNDEMVSFIAGISSDNDDVIAEQAAFLNMVKDLQM"
     gene            complement(12705..13193)
                     /locus_tag="U1099_000261"
     CDS             complement(12705..13193)
                     /locus_tag="U1099_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM4866611.1"
                     /translation="MQTKKMSMFLFFAYLLLLTWMIIFKMDLSIVYGRYGYASINLIP
                     FAGTAVYDGVLDFPEILFNIVSFIPFGIYMEMLFRKASWVTNLCLIMLVSLCFEVLQY
                     LLLLGVADITDLLANGLGGAIGINIMYVLTSIWREKAYVRMNIFCFVLTFFVILITYL
                     AM"
     gene            complement(13363..13779)
                     /locus_tag="U1099_000262"
     CDS             complement(13363..13779)
                     /locus_tag="U1099_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEM4866612.1"
                     /translation="MNYLQNYILSKSSYLPSDKLFILQKELEDLDDEALNVLMMVEMR
                     QPLVALILAIFFGEFGVDRFYVGNKELGFAKLIAFAVSFVTLFILIGFLLFLGLYLWK
                     FIDCFLIMRACKEANFERLMLQIHQYKAFQHSNQTF"
     gene            13913..14302
                     /locus_tag="U1099_000263"
     CDS             13913..14302
                     /locus_tag="U1099_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866613.1"
                     /translation="MRVIIHYPKPIKQLSSQKLPLLVDGKLAGTVTQGKILSLPITQP
                     SVTLSIRGDKKSAIQVKEGDRVQIMENSRYFILYYFSLAIVPIALLFNLPTLVKTILL
                     ILALAITLLGQAIFPKYIISTEKSSDH"
     gene            complement(14418..17105)
                     /locus_tag="U1099_000264"
     CDS             complement(14418..17105)
                     /locus_tag="U1099_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM4866614.1"
                     /translation="MSKEQKRDLFYTQGEEQVLSSMESSLDGLSSDEASKRLADYGRN
                     ELDEGEKRTLLAKFLDQFKDLMIIILIAAAILTVITEGSHGLTDAIIILTVVILNAAF
                     GVYQEGQAEAAIEALKNMSSPVARALRDNHVVEVDSKDLVPGDIVLLEAGDVVPADMR
                     LLEANSLKIEEAALTGESVPVDKDLSAVLAEDAPIGDRVNMAYQNSNVTYGRGLGVVT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLHQLSKVLTYAVLVIAAVTMAVSVFVRGEG
                     ILPALMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNSIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGQLVDAKEALDASNTTLRVMNFANDTKVDPTGKLIGDP
                     TETALVQFGLDQNFDVREVLVSEPRVAELPFDSTRKLMSTVHQQAAGNFFVAVKGAPD
                     QLLKRVTQIEENGTIRPITDADKQAILATNKDLAKQALRVLMMAYKYVDAIPELESDV
                     VENDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDP
                     NDTEDHVFTGAELNELTDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKT
                     IQYLLSANTAEVLCIFLATLFGWDVLEPVHLLWINLVTDTLPAIALGVEPAEPGVMQH
                     KPRGRNSSFFSGGVLSSIIYQGILQTALVLGVYGFALLYPEHAGDNHAIHADALTMAY
                     VTLGLIQLVHAFNVKSVYQSIFTVGPFKNKLFNWSIVAAFLLLMATVVIPGFNTFFKV
                     SILTPTQWMVTVIGSGLMVVVVEIVKFVQRKLGLDEKAI"
     gene            complement(17315..17701)
                     /locus_tag="U1099_000265"
     CDS             complement(17315..17701)
                     /locus_tag="U1099_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEM4866615.1"
                     /translation="MDIHLRNEIDLDGQIEVVDQQFQVEVKEKDGNLYLIYSNDEAEK
                     VVIKCDEEELVMTRFSTPKSIMRFISGKEAIVTIPTPLGIQHFVTDTKRYQLNRSDQS
                     VQLQYELKGLENQQLFASYDLEISWK"
     gene            complement(17713..18600)
                     /locus_tag="U1099_000266"
     CDS             complement(17713..18600)
                     /locus_tag="U1099_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEM4866616.1"
                     /translation="MNNQIILLTIQVFIVALILWMLYTILSYAKKHDISLKEKFWTGF
                     GIGYVTDLLDTLGIGTFATTTTMFKLTKLVEDDRKIPATMTTAHIIPVLVEALLFITI
                     VEVDMVTLIAMAAAAFTGAFVGAKVTQKWDTKKVQRILGILLIIAACFMVYRMITNPG
                     ADLTNEVKGLTGWKLAVGIAFDFMVGMLMSMGLGNYAPELIFFSLMGISPAVALPVMM
                     LNAAMIMTAGAKQFIQSGRVNWPGVPGIVVGGVLGVLTAAFFLSNLDINNLKILVVFI
                     AAYTGFTLLRSSFVTRIKK"
     gene            18699..20012
                     /locus_tag="U1099_000267"
     CDS             18699..20012
                     /locus_tag="U1099_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM4866617.1"
                     /translation="MIEKVFRDPVHNYVHVDHELIYKLINTKEFQRLRRIKQLGTTSY
                     TFHGGEHSRFSHCLGAYEIARRITQKFEDKYPQIWDTNESLLTMVAALLHDVGHGAYS
                     HTFERLFDTDHEEMTCAIITSPETEINALLKQVSPEFPDKVASVIKHTYPNKQVVQLI
                     SSQIDVDRMDYLLRDSYFTGANYGEFDLTRILRVIRPTENGIAFKESGMHAVEDYVLS
                     RYQMYMQVYFHPASRSMEVLLQNLLNRAKLLYTSEQEFFARTSPRLLPFFEHRIRLTD
                     YLSLDDGVMNTYFQSWIDGPDRILSDLAQRYINRKVLKSITFKAEEEEALDRLRSLVA
                     DVGFDPEYYTAIHHNFDLPYDIYRPNAEKKRTQIEIYRKDETLVELSSLSPIVHSLSG
                     TIHGDSRFYFPKEMLEETGIFAPQIADFNSHIHNDHFIIGESNEH"
     gene            20002..20811
                     /gene="yidA"
                     /locus_tag="U1099_000268"
     CDS             20002..20811
                     /gene="yidA"
                     /locus_tag="U1099_000268"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEM4866618.1"
                     /translation="MSIKLVAIDIDGTLLNSQHQITAEVHSAIQESKKAGVKIVIATG
                     RPISGVQAILEELNLTEAGDYVITFNGGLVQDAATGEDIVKDTLTYEDYLDIESAARK
                     LKLPMHASTKEGIYTANRNIGKYTIYESTLVSAPIFYRTPEEMADKEIIKTMMVDEPE
                     ILDAAIPLLPTSLTEKYNVAKSAPFYLEITPKTVNKGQAIIHLAEKLGLTMDQTMAIG
                     DQENDRPMLEVVGNPVVMENGNPELKKIAKYITKSNDESGVAHALREWVLK"
     gene            complement(20865..21617)
                     /locus_tag="U1099_000269"
     CDS             complement(20865..21617)
                     /locus_tag="U1099_000269"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEM4866619.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAQAFVEAIAGKLPAGDKIEAAIAAP
                     AVDLNALLWFAKDSELKVAAQNCYFEDAGAFTGETSPKVLAEMGVNYVVIGHSERRDY
                     FHETDEDINKKAHAIFRNGLTPIICCGESLETYEAGKAVEFVGAQVSAALKDLTAEQV
                     ASLVIAYEPIWAIGTGKSATKDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVNPS
                     NVAEYMACPDVDGALVGGASLEAESFLALLNF"
     gene            complement(21792..22988)
                     /gene="tuf"
                     /locus_tag="U1099_000270"
     CDS             complement(21792..22988)
                     /gene="tuf"
                     /locus_tag="U1099_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEM4866620.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGLQEEKSKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVISKPGSINPHTKFKGEVYILTKEEGGRHTPFFDNYRPQ
                     FYFRTTDVTGSIKLPEGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(23344..24315)
                     /locus_tag="U1099_000271"
     CDS             complement(23344..24315)
                     /locus_tag="U1099_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HEM4866621.1"
                     /translation="MTMKTFEEVTKKSKRKNLWKTIGLSSLIGFILMLIGVQTVKIIV
                     GAREGRENWKQIILMEISYPNIKHDGIKIQSTSYVSGKLYCNLYKDLAGVPMAFGQHE
                     IYYDLFDRHYDFAQHIPGNTFKYLYDFTTNTKVPVFYNRKKSVWSAQDIANELHYLKD
                     MSGQLVEVAISFDKKYSLAEIKQMIPSNLKQNWYWIGTEGKQNTHYYRPEQLLGLDPE
                     DIQLVFSDMPGDYMTGMDFMKEFLESGDQERAQYLDIPVDDLQAFVDKFGGTDFTKQE
                     EIDQLEFAGIILTGKAEDFAQLEGKDWIYSSSIGASIPIQPYYQLHH"
     gene            complement(24296..24799)
                     /locus_tag="U1099_000272"
     CDS             complement(24296..24799)
                     /locus_tag="U1099_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4866622.1"
                     /translation="MSIKLDQYEKEIIGIAEEISYYLQKSGAGPADSQDIAQDVLVKL
                     LESDIVLPFEKLRAWLYRSAVRAYIDKYRRDKRYHEILQKEFFRREDLLVYDQEGYEE
                     LYQAVADLPEKYQQVIDLYYFQDMSVKEVAHILGTGQSWVKIILYRGRKQLRKILEER
                     GYDYEDF"
     gene            complement(24914..27610)
                     /gene="ppc"
                     /locus_tag="U1099_000273"
     CDS             complement(24914..27610)
                     /gene="ppc"
                     /locus_tag="U1099_000273"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEM4866623.1"
                     /translation="MAIQKLENYNNKEAIREEVTILTSILEKVAAQMMPAETFAKIVE
                     LSQLSRQDDYQALIAIISQLNNDELAVISRYFAVLPLLINISEDVDLAYEINHQNNID
                     QDYLGKISTTIDMVSKQENAAEILEKLNVVPVLTAHPTQVQRQSMLDLTKHIHELLRR
                     YRDVKLGLLNKNKWEDELRCYIEIIMQTDMIREKKLKVTNEITNVMEYYNSSFIKAVT
                     KLQREYKRLAAEKGIVLNNPRPITMGMWIGGDRDGNPFVTAETLKLSALTQCEVIMNY
                     YDDQLYKLYRNFSLSTSIVNVSTAVKMLADLSEDSSVYRENEPYRRAFHYIQMKLANT
                     RDYLVHNKPSDVRYSNVAEFKADLLAIKQSLVENKSMALLKGDFTELLEAVEAFGFYL
                     ASIDMRQDSSIHEASVAELLASARIVEDYSSLSEEAKCHVLLKQLETDPRILSATHMP
                     KSEQLEKELAIFAAARELKDKLGEEIIKQHIISHSESVSDLLELAVLLKEVGLVDVDK
                     ARVQIVPLFETIEDLDNAPDTMRQYLQLDLAKRWIAGNKYYQEIMLGYSDSNKDGGYL
                     SSGWTLYKAQNELTQIGQDYGVNITFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRLR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSATLDRMVTQMITDPNEIDGYRETMDEIVSDS
                     YRIYRDLVFNNPHFYDYFFAASPIREVSSLNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QNRVMLPGWYGVGSSFKRFIDKHPDNLSKLQKMYESWPFFRSLLSNVDMVLSKSNMNI
                     AFEYAKMCESEEVRNIFHVILDEWQLTKEIILSIEQNDELLAELPFLKASLDYRMPYF
                     NVLNYIQIELIHRLRRGELSKEQENLIHITINGVATGLRNSG"
     gene            complement(27631..28845)
                     /locus_tag="U1099_000274"
     CDS             complement(27631..28845)
                     /locus_tag="U1099_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM4866624.1"
                     /translation="MKIDKRQLLNYSILIPYLILSVIGLVMVYSTTSATQIINGGNPF
                     RTVINQAGFWIVSLVAIYTIYRMKLSFLRKKAVIYSVILVEVFLLAISRLFPPINGAH
                     GWIPLPIAGTLQPAEYLKLIIIWYLAHEFAKQQADIRTYDYVSLIKGSWIPKEFTDWR
                     VVSLLLLGLVATLPDLGNATIIVLIMVVMISVSGIAYRWFSTAVLGIVAASTVILGTI
                     RILGVETVEKIPLFGYIARRFAAYFDPFGNATNSGLQLTHSYYAMSNGGWLGRGLGNS
                     IEKKGHLPEAHTDFAFSIVIEELGFVGASLILALLFFLIIRIIIVGVRARSPFNAMMA
                     LGMAGMLLIQTFVNIGGISGIIPATGVTFPFLSQGGSSLLIISIGIGFVLNIDASEKR
                     RLIEEEIERTLI"
     gene            complement(28982..29347)
                     /locus_tag="U1099_000275"
     CDS             complement(28982..29347)
                     /locus_tag="U1099_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEM4866625.1"
                     /translation="MKKISIMLMVVFSLFLAACTNTTEKQSESAIEVTLQITTKDKAN
                     HQEVTAEEGDSVMDVLKEHHDIVEDNGLITEIDGVSQDASTNTYWMYKVNGQMADKGA
                     KDLTVANGDEIEFYLETFE"
     gene            complement(29344..29847)
                     /locus_tag="U1099_000276"
     CDS             complement(29344..29847)
                     /locus_tag="U1099_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866626.1"
                     /translation="MHDKSEKVRRVSQLVVLSSLTIVLRVVFGPFPNIKPLTAIFLIV
                     LSYFSLLESWILMILVMVGSGLLFGFGTVVLWQVLSFAFIQFIWWLAIGPFIKKENIP
                     LWLQSLLAGILVFLYGLTISFLSATQFRTSPIVFWLNGLVFDSLHAASTVLFYPIIDY
                     IFRRFYK"
     gene            29960..30814
                     /locus_tag="U1099_000277"
     CDS             29960..30814
                     /locus_tag="U1099_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEM4866627.1"
                     /translation="MRKKLNPMIVVGFFLSFFALIFITGVTGNTVNKTEVANEPAVTQ
                     SNTSTSSKTTNSSSSYYIANALEMKPIVDVSAWQRPSEIDYDTLSKNISGAIVRIQSG
                     SHTKNENTATDKNGLDKSFDTHIKEFQARNIPVAVYAYVTGNSIENMREEARSFYKAA
                     NKYKPTFYWLDVEEYTMDDMNAGVEAFRKELETLGAKNIGIYVGTYFMEDHSINVDKF
                     DAIWIPTYGDDSGYYNAAPNTDLNYDLHQYTSRGYVNGFEHHLDLNIITTLKDPNEVY
                     QKLFTTPE"
     regulatory      30838..30933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            30992..31540
                     /gene="thiT"
                     /locus_tag="U1099_000278"
     CDS             30992..31540
                     /gene="thiT"
                     /locus_tag="U1099_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEM4866628.1"
                     /translation="MSTSKLPILAEVAIFSAIALVFDKIPLFTMPQGGSVSLVMLPIL
                     LLALRHGLGVGVLTGGIVGTIQLFYGGYFLNVFQVFLDYALSYAGIGLAGLVAPTLSK
                     QKDLKNATLIITLASFLGGSIRLLATFLSGIIFYADYAPDGMPVWFYSFTYNISYILP
                     STAIASILLILLYRARPGFYNL"
     gene            31643..32485
                     /locus_tag="U1099_000279"
     CDS             31643..32485
                     /locus_tag="U1099_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEM4866629.1"
                     /translation="MASWISRIIKGMIIALGFILPGVSGGVLAAILGIYERLIGFLAN
                     IRKDFKENFLYFVPVGIGGILGIALFSFPVEFLLQHFQVPVLWAFAGAIVGTIPSLLK
                     ESTQKSKRDSIDLAWLIGTFIISGLLLYNLSDLVGTLPATFASFVLAGALIALGVLVP
                     GLSPSNLLLILGIYTPMLNGFKSLDLFGTFLPIAIGGVLAMVAFSKAMDHALKVYHSR
                     VYHFIIGIVSSSTLLILIPQASNKESISYAGANFVTWIAAIVLFILGAWLGLWMSKLE
                     EKYK"
     gene            32485..32967
                     /locus_tag="U1099_000280"
     CDS             32485..32967
                     /locus_tag="U1099_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEM4866630.1"
                     /translation="MAKKVKIKKTLVDQILDKAKINHDSLVLNAFEGQLPPGIEEHEI
                     YKTLALTGDKTGPIIGIVPITEHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNFPIYIDQSALELGHLIVSAGEIGRSIRIDSQVLADFVKADFADLIEGRD
                     "
     gene            32970..33596
                     /locus_tag="U1099_000281"
     CDS             32970..33596
                     /locus_tag="U1099_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM4866631.1"
                     /translation="MKIYFVRHGKTEWNLEGRFQGYSGDSALLPESYQDLEKLGKYLA
                     EIPFDAIYSSDLQRAHSTAVEIAKANHHCQTVLTTQQLREWNFGTLEGSKMAIFRAIY
                     PKQAWALKHNLALFNNDLFEAESVRQVTQRMVDFVQSLKGQDMETVLIVSHGAFLTAS
                     IHRLLGFPPAQLRHRGGLDNASVTILETSDFENFTELAWNDTSYKAKQ"
     gene            33699..35189
                     /gene="lysS"
                     /locus_tag="U1099_000282"
     CDS             33699..35189
                     /gene="lysS"
                     /locus_tag="U1099_000282"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEM4866632.1"
                     /translation="MSTEHFEELNDQQIVRREKMTALAEQGIDPFGKRFERSANSAEL
                     KAQYEDKSKEDLEELGQTAIIAGRIMTKRGKGKAGFAHIQDREGQIQIYVRKDDVGEE
                     NYEIFKKADLGDFIGVEGDVFKTNVGELSIHARKLTHLSKALRPLPEKFHGLTDIETR
                     YRKRYLDLITNRESFDRFVTRSKIISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMDLTEGIIQHTAKAVVGDGPVTYQGTEIAIHEPFKRIHMVDAIKEQTGVD
                     FWQEMSFEEAKALAAEHKVPVEKHHTEVGQIINSFFEEYVEATLIQPTFVYGHPVAVS
                     PLAKKNDEDPRFTDRFELFIMTKEYGNAFTELNDPIDQLERFEAQAKAKELGDDEATG
                     VDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT        10779 a         7984 c         6462 g        10173 t
ORIGIN      
        1 tgtatattag gaaaatcaca cttggttaat tcaaaaaaac tagttaggat aatgtaaacg
       61 tcctaactag tttgcgatac atattctgct ggaactccta cagagcatag gcctgtaatt
      121 ctggattgat aggagtgttg atgagattgt tggcataatt acaaagagtt gcaagactga
      181 ctcctaacac cacatccaag gcattttcag ccgtgtagcc ctcttggaaa aattcctcta
      241 gcaagggctg ttcaaccttg cctttttcat ggataacagc cagagtaaat cgagcgagag
      301 catccaattt tgcatccgtt tcgatgggag ttccctggcg tagagcctgt agaataacat
      361 caggcatctt aacctgcttg atagaaatag ccgtatgacc tgccacgcaa aatccacagc
      421 cattggcaac ggcagctgta atttgtacca cttcacgctc cgtcggattc agactactac
      481 ggcgattgat accactaaca gtttggtagg tttctagggc tgtaggagaa ttggctaaaa
      541 gaccgatgag attaggaata taaccgccat tatttttctt gacagtcgca aggttttctc
      601 gtaattctgg actaacagtc tctagagtat ggataggaaa gaaagcttgt gtcataggta
      661 actccatttc aaaaaatttc atagagtagg aataaaaatc tgcttggatt cacacctata
      721 cttctatgct tgcattttat actatcataa agacaggcat cctgctaata tattttacct
      781 atcacaccta taaaaaattg ataatccctt gtagaaacca ctagaaagac tggataactt
      841 cgaccagttc atcccaatct gtcaccactt tatctgcctg atacggagct gttggctcta
      901 ctctctttct atgattaaac caaattgcct tccaattcgc agctttcgct cctaccacat
      961 cattttcaaa cgaatcccct agataacaaa tatcttcgcc tgacatcccc aaacgttctt
     1021 ccattagttg aaaaatagca ggatttggct ttgtgatacc aacttgtcca gaaattagga
     1081 catgttctag ctcaaaccaa ttaactaacc ccatcgtgcg tatctttctc aactgatgtc
     1141 tataaggtcc attcgtaata atccccattg caattccttg cgcctgacac catgaaaata
     1201 tttctggaaa cactggactg agctctatct gcccttgaaa ataatcataa tctatttgaa
     1261 tagctaaggc tgttgcatca gacacctgat acccaaaatc tgccaaagca tgtttcatcc
     1321 gataaatatg actgtctttc agactcatct ttccaattgc tgtcgcttca aatacttcat
     1381 ccgcatatct gcgaaaggaa aggtagagtg gttcaatttg ctcccttgct acttcaatgt
     1441 gtctttccaa agcacgttca aacggttgaa tctgatcata aagcgtatca tctacatcaa
     1501 aaattaaggc tttcatgagt caattatagc aaaaaattcc agccaaagct tggaattttt
     1561 aactgcttat tacacctttg cttgaatctc atgcatttga gcatatactc caccacgtgc
     1621 aaccaattct tcatgcttcc catgttcaat aattcggcct tgatctaaga caagaatctg
     1681 gtctgcattt tgaatggtag aaagtcggtg ggcaatgata aaggttgtcc gaccttcttt
     1741 caccacttcc atagcatgtt ggataatttc ttccgtctcc gtatcaatat gagaagttgc
     1801 ttcatccaat atcagaattt taggatcaga gtagagtgtc cgcgcaaagg caatcaactg
     1861 acgttcccca cttgaaaagg ccgccccctt ctcaacaacc ggttcatcaa tccctttctc
     1921 taagcgagat agcataggcc ctgcaccaac tttttctaac gcctgcatga ttcttgttct
     1981 atctgcttcc tcttcattca tggaaacatt gctagcaatc gtccctgtaa agagataggg
     2041 atcttgcaaa acaatcccca tgtgactgcg caggctctca cgagaataat cacggatgtt
     2101 tttcccatca atcagtacac gtccctcctg tggatcatag aaacgataga ggaggttcat
     2161 aatagaagac ttaccagacc cagtatgccc aaccagagca actgtttctc ctttttctgc
     2221 ccgaatggta atatctttca aaactggtct gttttcttca tagctaaagt tcacgtgttc
     2281 aaacacgact tgcccttgtt caacttccaa aatacgatca cagtcgtcct ccacctcttc
     2341 ttctagaagg gccattagac gcttacctgt ttcaaacgag cgaagcatat ttgggaactg
     2401 ttgaaccagc gctcccaaag caatgaagac tccttcgata tagttgacat aaacaaagag
     2461 tttcccagct gtaactccat actgtcccgc aagaaactga tagccgacta ccgttaaaat
     2521 cgcagcaatg accaaatttt gaagaaaacc actcagattc cacgtggcaa gagactgggc
     2581 ccagataatc ttattatctg cccgacgcat cttgtcagca gtcacttcaa attctttcat
     2641 gatgcgctct tcttgtccaa aaagttgaat caaacttgca ccattcatgg tctcattcac
     2701 ctgcgtattg acgtcactcc ttgcatcata aaaatccttc atcggcttgt ctgtcatttt
     2761 cttgtaagca aattgaatac cgatatagag tggtatcaag agtaacatca gccaaccaag
     2821 actcctattc atgtaaaata ggacaccgta tgtaaagagc aaacgaacaa tattgttaaa
     2881 ggcattaact aaggtcccat aaaattgtgt ccgcagagtt tctgtatcat tgacaattct
     2941 agttgcaatt tttccagcgg gcttatcatc aaaataagaa ataggcaacc gttgcataac
     3001 atcataagct tgattacgta gattttcagc aatacgattg gcgcaatgca tgagtagacg
     3061 catagataag tagaagccaa tagctcccag aataatcatt cccatgtatt gagctgactg
     3121 gtttaagaaa acagcttcat caaaaggcaa acccttgctc aaatccgtaa tcggcccatc
     3181 aatcgcctgc tgaatcagta gaggagccaa tcgaaccatg gtcgaagcca atagatagaa
     3241 gccgacagct actataaata gccacttaac acgacggatt tgttttaata aaaaacctaa
     3301 aatagtcatt actctcctcc ttcctgactt tgttgtcgtt ggtattgttc atagtaccaa
     3361 cctctttggg caagcaaatc agctggacgc ccttcctcaa cgatacgccc ctcatccaag
     3421 actagtaccc agtctgcatg gttgacagca gatagacgat gggtcacgat gacattggtt
     3481 ttgcctgcac gttctttttg aatattttgg ataatctggc gttctgtcct agcatccact
     3541 gccgacaaag aatcatctag aatcagcaaa tctggctctc gcaggaaagc acgggcaatg
     3601 gaaatccgtt gtttctgacc gccagaaata gacacaccac gctcaccaat catggtatca
     3661 agaccatcag tcatccgttt taaatcttga ctaaaagctg ctgtggcaat ggcttgttca
     3721 atctcctcag agctactagc tactttccca agggcaatat tttctccaac agaccttgaa
     3781 aacaaaatgt gttcttgggg aacatagcca attttttcct ctatactaga acgtttaaag
     3841 tctaaaacag attgatgatt gatgaaaaaa tttccctgac caatcggata ctggcgtaag
     3901 aattggcgaa ccaaggtcgt ttttcccgaa ccagttttcc caacgatacc tacagtttgc
     3961 cctgctttca gtgtccaatt gatgtcctgt aggctggctc tttccgcctg tggataacta
     4021 aagctataat ttttgaagga aatactagac agctctgcaa tttccttaga accatcagcc
     4081 tccaaatcat cacccgtctc aatcagctct tgcagttttt cgaaagatgt cttaccagtc
     4141 tgataaacaa ggatgaaatc tgctagtgtc caaaacggct ccaaaagcga accgacatac
     4201 aattgaaggg caattacttg tcccaaggtc agttgccctg ctttcacggc ttgtgccccc
     4261 atgagaagca cgaaaatagt agacaatccc aaacagactg tggctaatgg attatacaat
     4321 gattgcaagg aagtgatgcg atccccacct tgagccaatt gcttggtacg agcttgaaat
     4381 tgagcctttt gagttgcttt tttactataa gcccgagtca ctcgaatgcc ttcgatgacc
     4441 tccaaaactt cattattgag ctgggctaca gcttctctat tggcatcaat cgccttatct
     4501 tgttttctcc cgataaaaaa tatacatagt gtcatgaaca gcatcggtag taaagctact
     4561 agcgaaattt tccaatcaat aaagaacatc gtgggaatga taaaagccaa cataccgcct
     4621 gcatagacga caatcataag accgtaaccc accatctcca tcaggccatc cacatccgtt
     4681 gaaaaacgag tcatgacatc tcctgagcgg aatttttcat aaaatggagt ccgcatggtc
     4741 actaattttt taaaggcacg ttgctgcata tcaaacttga aattgacaga agcctgaaat
     4801 aatttcagat gccaaatgaa ggctgttgca tagttcagta ctgttaccaa taacaataag
     4861 gtcatctcct gaaccaatat ggcctgtgtt aattcattct tggttagtat atctaccatc
     4921 cgctgaatga tttgcgttgg tagcaaaagt gtggcgtcat aaataatcaa ggtcacagca
     4981 accaatagat agagccactt atgacgcttg atatagtcaa aaattaatcg aaacatatat
     5041 ctcctttaca aatctataac ctatcatttg ccgctttatt caataagcaa caattgatag
     5101 acgttatact caatgaaaat caaaagtagc ctagaaaacg atgtcgaaga tagaactgga
     5161 gttcatcaag gcaagttgac aacggataat tttgattttc gaagagtatt acctttctaa
     5221 gcgcacaaaa aagcctaggg aagcatctca ctccacctag gcttgcgtaa gtccacaaaa
     5281 atagaccagc tcttgaacat agtcgacaat ttttatcaca ccatacccac agtgcttgat
     5341 tgtctcaatg tttcaaaata tagcccacga aaaacgacaa aagttcatca atgagctttg
     5401 ttttcctgct tagaaaggag tgtatgataa aaaatagctt gctgaataac aagttgcttg
     5461 ttcatttgtt ttactccttt catatttcta agccctattc taccataata aacattcatg
     5521 tcaagggatt ttttgacaat aagggcgaat ttaggacaaa aataagaaaa accttgtcaa
     5581 acaaggcctt tcgatagtat tttatgctag ttgccgggat tgaaccggcg acctcatcct
     5641 taccatggat gcgctctacc gactgagcta aaccagcagt acttgactag tatatcatat
     5701 ctctaaaaaa agtcaatgga ttttttggaa atattgagaa aaataaagag aaaataagcc
     5761 attcctaaaa attgtctgac acttttaagg aattgtgcta tactattgtt ataaaaatca
     5821 caaaggaaac gatatgaaac tgcaaaacct tcaagttgga caatcttatt tagtcaaaga
     5881 ttgcctaacg caagatgata ttcgtaaaca cttagcgcac ttgggtttaa aagtcggaga
     5941 agagattcgc atcatttcaa aaaccaagac caatgcaatt ttccaagtca aggcaagccg
     6001 tctagcccta gacagagaaa ttattgaatc tctggtttta acagaaaagt cagctacgga
     6061 aataatcaat ctatctgagg cacctattgg tagctcagcc aaggtcatgg acatctatgc
     6121 aacaggtgca ctccgtcgtc gcctcatgga tatgggactt acaaagaaca cacagctatt
     6181 tctcaaaaaa gtcgctcccc taggcgatcc aatagaaatt accttgagag gatacgagct
     6241 gacactacgc aaatccgaag cgcaaatgat tggtgttcaa atcacaagcg aggtgaggaa
     6301 atgacaaaac acatcgccct tgcaggaaat ccaaatagcg gaaaaacaac tcttttcaac
     6361 ctcctaacag gaaccaacca acgtgttggt aactggcccg gagtcaccgt tgaaagaaaa
     6421 tctggaacca tcaaaaagcg aaaacattta ctaattcaag atttaccagg tatctactcg
     6481 ctgtctccct acacaccaga agaacgggtt gccagagatt atttgatagc ggaccaacca
     6541 gctgctatcc tcaacgtcct tgatgcaaca aacttggagc ggaatctcta tctgaccttg
     6601 cagttattag aaatgggcct tcctatagct atcgccctca atatgacaga cgccctgaaa
     6661 agtcaaggtc gctcaataaa cagcgaccag ctctcctatc aactgggagt tcctgtacag
     6721 cctatcagtg cactaaaaaa actaggtata tcccagctcc tccatgaagt agaaaaaatt
     6781 accaatcagc aagccgagcc aatctatcct cagtatgata agcaatttga agccggtctt
     6841 gcccaagtta ttgacctatt atcggattcc atcccaaatc atcaaaaacg cttctacgcc
     6901 attaagctac tcgaacaaga tcaagtcatt ctagaccaat tgcaactaaa tgcacaagac
     6961 atgcttgatt tgaccgaaat cattgccatt ttggaaaaaa tttacgcaga cgatatggaa
     7021 gccattatcg tcaatcaacg ctaccaattt atcgagaaaa tcacagaact ggtcacaaaa
     7081 gacagcgata aggcttttaa cctgtctgat aacatagacc gacttgttac caatcgtttt
     7141 cttgccctcc ctatctttgc ggcagtcatg tggctgacct acttcctgtc tattcaaacg
     7201 gtaggtacaa tggggactga ctgggtgaat gatgtcctct tcggagagct tgttccaggc
     7261 cttattcagg caaatctaga tcgatttgaa atcgctggct ggctacaatc tctggtcctt
     7321 gatggcatta ttgcaggatg cggagccatt ctaggttttg taccacaaat tttcgtcctc
     7381 ttcgtctgct tgggaatctt ggaagacatc ggctatatga gccgagtcgc ctttgtcatg
     7441 gataggattt ttagacgttt tggtctatca ggcaaatcct ttattcccat gttgatttcc
     7501 acaggctgtg gcgttcctgg agtcatggca agccggacta ttgaaaatga acaggaccga
     7561 aaaatcacca ttatgacagc aaccttcatg ccctgttctg ccaaacttcc tatcatttca
     7621 ctggtagccg gtgctttctt tcctgacaat ccttggattg ctccaagcgc ctattttgtc
     7681 ggtatggctg ccatcgtcct atccggaatg gcactaaaaa agaccaagca acttggtgga
     7741 gtcgcaagcc cctttatcat ggaattacca tcttatcact taccaaaact cagtacggtt
     7801 ctccggtatg cattcgataa ggcacttagt tttatcaaac gagctggtac cattatattt
     7861 gtgaccaaca tcattatttg gttcagcagt tcctataatt ggtctttaca aatggtagaa
     7921 acagatgaga gtatcttagc ctccattgga cacagttttt ctctcctctt tgccccactt
     7981 ggtttcgaaa attggcgagc aactgttgca gccattacag gtctcttagc caaggaaacc
     8041 gtcattgcca cctttggtat cctctataaa ttaggagaaa caaccgaaga aaatcccgaa
     8101 ctatgggggc ttttacaaca agactataca gcgttatcag cctactcctt cctagtcttc
     8161 aatctcctct gtgccccttg ttttgcggct attggtgcca ttcaccgtga aatgggagaa
     8221 gccaagtgga catggattgc tatcggtttc caaacgggtt tagcctatgc aagtagtttg
     8281 gttatctatc agattggtct tgtacttatc cacggacaat taccaaccgt ctggacattt
     8341 atcgctatct ttctaattat cacagccatc tacagcctag tgaaaaaacc aaccaacact
     8401 cttccaatcg tcacgctaaa aacattagaa aaaggagaat attaatgccc acacttatcc
     8461 tctttctcat catcgtcatc ttattttctc ttgcgattcg cagtctcatc aaaggaaaag
     8521 gatcctgcgg agattgttcc tgcgactgta ctattaagca agagatgaac gaatcttctc
     8581 accatagaca tccataacaa aaaggtctgc ctcattattg tgcagacctt tttacatgta
     8641 gcttgtctat taaattttta agtagcggtt catggaaatg actgatccca aggaaccaat
     8701 aataatacca attacaaaaa gtgcgccaat cattcctgga acaaagacat ccatactaat
     8761 cagagacaaa tcttgactag caagactagc atttaccgaa gtataaatca tcttataaaa
     8821 agaatagact aaagcagaag gcaaaatagc tccgaggaga cctacccagg caccttccca
     8881 gaggaatggt ccacggatat aactgttttt agcaccaacc agacgcataa tctgaatttc
     8941 acggctacga gaaataatgg taattcgaat agtattggaa atcaagaaaa cagcagtaaa
     9001 gagcaataag ccagttgctg ccaatcccca tgtacgaact agattagcta atttaaaaat
     9061 acgttccgtt tcaacttccc catcccgtac ctctgtaaca ccatcaattt tagcaatctc
     9121 tgctgcaaca gtttttacat attgcggttc agtcgtatca atgatataag catcgtaaag
     9181 tggattggca tctccttgga ataggttcca agtatctccc aaggtagctg ttaatttctg
     9241 taactgttca tccttacttg aataagtgac agattgtaca ttttctaagg ctgtaatctg
     9301 attatacacc tttttataat ctggattagc taccgtcttt cctgcttcgt tcacaatcgt
     9361 ctcagcttga tcggttgagt tcgctctcaa atacacattg atgcgtacat tctgttccaa
     9421 atccgaagcg agctttgctg tgttcaaaat aactgaagca aatagaccaa ccaaggtaag
     9481 ggtaatggct acagatgaaa tggctgcaat cgtcatccaa ccatttcgct tcaaactctt
     9541 caatgactca ataaagtgtc taaaaaatcg attactcatc gtatccatat tctccttccg
     9601 cttcatcacg tactacacga ccatcttcaa tcgcaataac acgatgacga agtgtattta
     9661 caatctgact attgtgtgtc gccatcaaaa tcgttgtccc ctgtaagtta attcgctcca
     9721 atagattcat gatttcccaa gagttttcag ggtccaagtt accagttggt tcatcagcaa
     9781 tcaatacttt aggattattt acaatggcac gggcaatagc aatccgctgt tgctcaccac
     9841 ctgaaagttc atttgggaaa gaacgaattt tgtgcttcaa accaaccaaa tctaaaactt
     9901 ccattacacg tttcttgata ttccgtggtt tctcaccgat aacttccatc gcataggcaa
     9961 tattttcaaa gacagtcttt ttaggtaaga gtttgtaatc ttggaagacc actcctacac
    10021 tacgacgtag aagaggaaca tctctctttt taatttttgc aaggtcaaac ttaccaacct
    10081 ttagactccc cttatcgagc ttttcctccc gatacaagag tttgataaag gttgacttac
    10141 ctgcaccaga aggacctaca atgtaggcaa attctcccgc ttcaacattg actgatactc
    10201 cgcgaagggc agtcgttccg tttccatatt tcttggaaac gtctttcatt tctattattc
    10261 ccataggatt tcctgtctta gacagtcctt tctttagatt tgagaacgtt tttttcaaaa
    10321 tacagtccgt attctctaaa aaaacttcct tgatgagcga aaaactatct cttatttaca
    10381 agcacttcac ttgtaaatac aggttcttag ctaattcgcc attttaaata agcatctata
    10441 aagccgtcca agtctccgtc cataacctta tcgacttgag ccacctcata accagtacgg
    10501 tggtctttta ccattgtata aggtgtaaag acataagaac gaatctgact tccccaagtg
    10561 atctctttct tgtcaccctt aagtgagtct acttctgccg ctttcttctc ctgctccaat
    10621 tgatacaatt tagcctgcaa gagtttcatt gcacggtctc ggtttccata ctgagtacgg
    10681 tcaactgtgg actgagtcac aatccctgtc ggaatgtgcg tcaatcgaac acccgttgaa
    10741 accttattga cgttctgtcc accagcacct cctgaccgga aggtatccat cttcacttca
    10801 tcatctctga tttcaatttc aatggtatca tccaactcag gcattacctc cacagatgta
    10861 aaggaagtat gacgacgctt agctgagtca aaaggtgaga tgcgcaccaa acgatgaaca
    10921 cccatttctg acttaagcaa gccataggcg tttggaccag taaagcttag agtcacagac
    10981 ttaatacctg cctcatcacc agcttgataa tccaatgttt ctactataaa acctttggca
    11041 tttccataac gctgatacat ccgcaggagc atttctcccc agtcttgtgc ctcggtacca
    11101 cccgaacctg gatggatttc caagatagcg ttattgtgat cataggggtc tgataagagc
    11161 aaggtcattt cgtaagcagt catggttttg tccaattcca ctaacttttc aaccaactca
    11221 tcccgcacag actcatcttc tgctagaaaa tctagcaaaa tttcagattc atcatataaa
    11281 tccaacatct gatggaaatt tccataagta ttttttaatt cattcaactc ctgagacgtc
    11341 ttctgggctg ccaaattatc attccagaaa tctggctccg tcatcttgtt ctcaagaatg
    11401 gcaatctctt cttccagacc ttctaagtca aagagacccc ctaaaagaag ttaatttttt
    11461 gccaagttct tcaatctttt ggcgaatttc tgctgtatcc atatacacct catttttatt
    11521 cataatcttt attatatcac atttgcaagt ccttgaccat gttgagaaag gccgcttgct
    11581 ctgctatgac atcatcattg tctgatgaaa tacctgcgat aaaggaaacc atctcatcat
    11641 tgggctgttt gaccaattgc cccaaggccc agatagccgt tgccatgtgg accggatttt
    11701 gttttttatc gatgatttct aggagtttgg gaatggcaga gcgatcatta ctattagcca
    11761 aggctatgat ggcattacgc tggagaatat ttttcccccg ccaagaacct gcaatcatac
    11821 caaatttttc cttgaattgt ccatttgaca agtccaagaa tggaatcagc tcaggctcag
    11881 ccaaatcagg gtcaatctcc accacaggtg gactagaaat tcccttgttg taaggacaac
    11941 aaatctggca aatatcacaa ccatagatga ccgtcttgat tttcttacga aactccaagt
    12001 ccatcatacc cttatcctgg gtctgaaagg acagacagcg acgggcattc atggtcgtgt
    12061 cacctaatag acaagacgtc ggacaggctg caacacagcg attgcaatca ccacaatcat
    12121 aatctactgg ttgatctggt tcgatgtcca ggttggtcac taattctcct agaaacatat
    12181 aggaaccaaa ttccttagaa atgactagcc cattcttacc gataaagcca attcctgctc
    12241 ttctggcaac agctgtatcc accaaagctc ctgtatctac catagccttg tattccaaac
    12301 catccgtcag tctctcaatt ccccgtgcca aacgctccat cttatcttgc aagatatagt
    12361 gataatcaag cccccatgaa ctaggggtaa tcttcccacg tttgtattgg gtcttctgcg
    12421 gtttaacagg caaatgtcga ggataagcta ccgcgataga aatgatggtt ttggcagatt
    12481 ctaacgacag tttgggacgg atacgttctt caatattctt gtgttcaaat cctgacgaac
    12541 gcccttcttc ctgactagca cgaagtgatt tctccaaata agcaaaatca tcagccgttg
    12601 taaaaccaat cttcgagatt ccgatttcct gagataattt aataatctct tccttaatat
    12661 tcattatatt cattttagca aactttgaat atatttagca agacctacat ggctaaataa
    12721 gttattaata taacaaaaaa cgttagcaca aaacaaaaaa tattcatacg aacataggcc
    12781 ttctcccgcc aaatactggt caagacatac atgatattaa taccgattgc tccccctaag
    12841 ccgttagcca gtaaatcagt tatatctgca accccaagta aaagaagata ttgcagaacc
    12901 tcaaagcaga gactcactaa cataatcaaa catagattag tcacccacga tgccttgcga
    12961 aatagcattt ccatataaat accaaaggga ataaaactta caatattaaa tagaatttct
    13021 ggaaaatcca gcactccatc atagaccgct gttcctgcaa acggaattaa gtttatacta
    13081 gcataaccat agcgtccata tacgatgctc aggtccatct taaagataat catccaagta
    13141 agtaaaagga gataggcaaa aaataaaaac atactcattt tctttgtctg catagaaatt
    13201 ctcctctcaa ctagcatatt ttatcattat atcataaatg ctgttctata aaatgtcact
    13261 tctgtgacaa agctcgttcc atattacaag tactttacag cctactaaaa acagaaaagg
    13321 aagacactac caagcctccc cttattccca aaaatatctt acttaaaatg tctgattgct
    13381 atgctgaaaa gccttatatt gatggatttg tagcatcaaa cgctcaaagt tcgcttcctt
    13441 acatgctcgc atgatcaaga agcaatcaat aaacttccac aaataaagtc ccaaaaataa
    13501 caagaaccca atcaagataa atagcgtaac aaaagatact gcaaaagcaa tcagcttggc
    13561 aaatcccaat tctttattcc cgacataaaa acggtcgaca ccaaactcac cgaagaaaat
    13621 agccaaaata agagcgacaa gaggttgacg catctcaacc atcataagaa cgtttaaagc
    13681 ctcatcatct aaatcttcta gttctttttg cagaatgaat aacttatcag atggtaaata
    13741 actgctttta cttaaaatat aattctgaag ataattcata taaactccta cctcttcaaa
    13801 attatagcag tttcttattc ttcaagcaag cctgtagggg aaaacagtgt cagaaaccgt
    13861 ttacattttt tctttttttg ctataatgaa actagattac aaggaggttc atatgagagt
    13921 tattattcat tatccaaaac ctatcaaaca actatcctcc caaaaacttc cgctcctggt
    13981 agatggaaaa cttgctggga cagttactca aggtaaaatt ctaagcttac caattacaca
    14041 accatctgtt acactaagca ttcgagggga taaaaaatca gccattcaag ttaaggaagg
    14101 cgatagggtc caaataatgg aaaatagcag gtacttcatt ctctactact tcagcttggc
    14161 tatcgtacca atcgccttac tgtttaactt accaactctt gtcaaaacca tcctcctcat
    14221 actagcatta gccattacgt tgttgggaca agcaatcttc ccaaaataca ttatcagtac
    14281 agaaaaaagc tcagaccact aaatctgagc tttttcaaat gatatttgct tttagtacaa
    14341 ggtaacgagg cgcaggctgt actgaagtac ggcaagtcga gttaacgaag taataaaaga
    14401 taagctgaat aactatatta gatagctttt tcatccaatc ccaacttacg ttgaacaaat
    14461 ttaacaattt ctacaacgac aaccatgaga cctgaaccga ttactgttac catccattga
    14521 gttggtgtca agatagatac cttgaagaag gtattgaatc ctggaatcac aacagtcgcc
    14581 atcaagagca agaatgcagc tacgatagac cagttaaaga gtttgttctt gaatggacca
    14641 acggtaaaga tagattggta aacagactta acgttgaagg catgcaccaa ctgaatcaat
    14701 ccaagagtaa catatgccat cgtaagagca tccgcatgaa tggcatggtt atctccagca
    14761 tgctctgggt aaagaagtgc aaaaccatat acgcccaaga caagtgctgt ttgaaggata
    14821 ccttgataga taatcgaact caagacacca cctgagaaga agcttgagtt acgaccacga
    14881 ggtttgtgct gcataacacc tggttcagca ggttcaacac caagggcaat cgctggaagt
    14941 gtatctgtta ccaaattgat ccaaagaaga tgaactggct ccaatacgtc ccaaccaaag
    15001 agggtagcaa ggaagataca caatacctca gcagtgttag ctgacaagag atactggatg
    15061 gttttttgga tgtttgagaa gactttacgt ccttcttcaa ccgccacaat gatagttgca
    15121 aagttgtcat ctgcaaggac catatcagag gcacctttcg aaacttccgt accagtgata
    15181 cccataccga taccaatgtc ggcagtctta agtgatggcg catcgttaac accgtcacca
    15241 gtcatggcaa caaccttacc atcattttgc caagccttaa cgatacgaac cttatgttct
    15301 ggagatacac gagcgtatac agaatattgt ttgaagactt tttggaactc ttcatccgtc
    15361 aattcgttga gctctgcacc tgtaaataca tggtcttctg tatcatttgg atcgatgata
    15421 ccaagacgtt ttgcaatcgc ctcagctgta tcttggtggt cacccgtaat catgattgga
    15481 cggataccag cttccttagc aacacgaaca gcttccgccg cttcaggacg ctcagggtca
    15541 atcataccaa ccaaacctga gaagacaagg tcgttttcaa caacgtcaga ttccaattca
    15601 ggaatagcat caacatactt gtatgccatc atcaagacac gaagggcttg tttagccaag
    15661 tccttgttag tggcaagaat ggcttgttta tccgcatcgg taatcggacg gattgtaccg
    15721 ttttcttcaa tttgcgttac acgtttgagc aattggtctg gggcaccttt aacagctaca
    15781 aagaagtttc cagcagcttg ttggtgaaca gttgacatca atttacgtgt tgagtcaaat
    15841 ggcaattcag ccacacgagg ctcagatacc aagacttcac gaacgtcaaa gttttgatcc
    15901 aaaccaaatt gaacaagtgc cgtttcagtt gggtcaccga tcaatttacc agttgggtca
    15961 acttttgtgt cgttggcaaa gttcatcacg cgaagcgttg tattgcttgc atccaaagct
    16021 tctttagcat ccaccaattg accgtttgta taaacttttt caacagtcat ctggttcata
    16081 gtcaaagtac cagttttatc tgaagcgatg atttcagttg aaccaagtgt ttcaacagca
    16141 ggcaacttac ggatgatgga attacgctta gccaagacct gtgtaccaag tgaaagaaca
    16201 actgtaacaa tagctggcaa accttctgga atggcagcaa ccgcaagagc tacagaagtc
    16261 ataagagcag gcaagatacc ttctccacgt acgaatactg aaacagccat cgttaccgct
    16321 gcaattacaa gaactgcgta tgtcaagact tttgatagct ggtgcaagtt ttgcttcaat
    16381 ggtgtatcag tctcatctgc attagcaagc atgccagcga tgtgcccaac ctctgtgtac
    16441 atacctgtat ttgtaaccac accaagacca cggccgtaag taacattaga gttttggtaa
    16501 gccatgttga cacggtcacc gattggtgca tcttcagcca aaactgctga caaatcttta
    16561 tcaactggta cagactcacc tgtaagagct gcttcttcga ttttgaggga gttagcttcc
    16621 aacaaacgca tatcggcagg gacaacatca cctgcttcaa gcaagacgat gtcacctggt
    16681 accaagtctt tagaatcaac ttcgactaca tgattgtcac gaagcgcacg cgcaactggg
    16741 cttgacatgt tttttagcgc ctcaatagct gcctcagctt gaccctcttg ataaacacca
    16801 aaggcagcgt tcaagataac cacggtcaag atgataatgg catctgtcaa accgtgtgaa
    16861 ccttctgtaa tcaccgtcaa gattgctgct gcaatcaaaa tgataatcat caagtctttg
    16921 aactgatcca agaatttagc taagagtgtg cgtttttcac cctcatccaa ttcgttacga
    16981 ccgtagtctg ctagacgttt gcttgcctca tcactagaca aaccgtctaa agaagactcc
    17041 atgcttgaca atacttgctc ttcgccttgt gtataaaaca aatcgcgttt ttgttctttt
    17101 gacaatttcc ttctcctttt cggcctcttc tctaccttct acaataaaaa gagacctgtt
    17161 ttattaaaac aagtctcgct atttaatata ttgccggaaa caagtcggat tgacgacaat
    17221 atcacggaat tttccgctag ctactccctc gatttgttaa ctattatatc aaaaatcccg
    17281 agaaattcaa gaatattttt ctggaaataa gagattattt ccacgagatt tccaaatcat
    17341 aggaagcaaa aagctgctgg ttctccaatc cctttaattc atattgcaac tggacacttt
    17401 gatccgaacg gtttaattgg taacgttttg tatcggtcac aaaatgctgg attcctaacg
    17461 gtgtaggaat ggtcacaata gcttctttac cagaaatgaa tcgcataata gacttcggag
    17521 tggaaaaacg tgtcatcact aactcttctt cgtcacattt tatgacaact ttttcagctt
    17581 catcgttact ataaatgaga tagagattcc catctttttc cttaacttcc acttgaaatt
    17641 gctgatccac tacctcaatc tgcccgtcca gatcaatttc atttcgtaaa tgaatatcca
    17701 tagttcctcc tactattttt taatcctagt aacaaaggat gaacgaagca gagtaaagcc
    17761 tgtatacgct gcaatgaaga caactaaaat tttcaaatta ttaatatcca aatttgatag
    17821 gaagaaagcg gctgtcaaaa cacctagcac cccaccgaca acgattcctg gaactcctgg
    17881 ccagtttact cgtcctgatt ggataaactg cttggcacca gctgtcataa tcatagcagc
    17941 attcaacatc ataactggaa gagcaaccgc tggactaatt cccatcaatg agaagaaaat
    18001 taactcagga gcatagttac ccagtcccat actcatcagc attcccacca taaaatcaaa
    18061 ggcaatacca acagccagtt tccaaccagt taagcccttt acttcattgg taagatctgc
    18121 gccagggtta gtaatcatgc gatagaccat gaaacaggct gcaataatta gtaaaatccc
    18181 aagaattcgt tgaactttct ttgtatccca tttttgagtt accttggcac caacaaaggc
    18241 acccgtaaat gccgctgctg ccatagcaat cagagtgacc atatcaacct caactatcgt
    18301 aataaacaat agcgcctcta ctaagactgg tatgatgtgc gccgttgtca tggtcgcagg
    18361 aattttacga tcgtcttcca ccaactttgt cagtttaaac atagtggttg ttgttgcaaa
    18421 ggttccaatc cccaaggtgt ctagcaaatc agtaacataa ccgataccaa atcctgtcca
    18481 aaatttctct tttagagaaa tatcatgctt tttagcatag gatagaattg tgtaaagcat
    18541 ccaaagaatc agggctacaa taaagacctg aatagttaat aagataattt gattattcat
    18601 ttctccatta taccaaaaga ttccattggg tacatagcaa tatccttgtg ttttagaaaa
    18661 atcaggaatt tatctccctt ttttgttata atgaaactat gatagaaaaa gtatttcgcg
    18721 atccagttca taactatgta catgttgatc atgagctgat ttataaactt atcaatacaa
    18781 aagaatttca gcgactacgc cgcatcaagc aactagggac tacttcctat accttccatg
    18841 gtggcgagca tagccgcttt tcgcactgcc tcggcgccta cgaaatcgcc agacggatta
    18901 ctcaaaaatt tgaagacaag tatccacaaa tctgggatac aaatgagtcc ctactgacca
    18961 tggttgctgc gctactccat gatgtcggac acggagccta ttctcatacc ttcgagcgtc
    19021 tatttgatac agaccatgag gaaatgacct gtgcaattat tactagtcca gaaacggaaa
    19081 tcaatgctct gttgaaacaa gtttcacctg agtttccaga caaggttgct agtgttatca
    19141 aacacaccta tccaaataaa caggtcgttc aactcatttc gagccagatt gatgttgacc
    19201 ggatggatta tctattacga gattcctact tcacaggtgc gaactatggt gaatttgacc
    19261 taactaggat attacgtgtc attcgcccga cagaaaatgg aattgccttc aaagaatctg
    19321 gtatgcatgc agtagaagac tacgttctca gccgctacca gatgtacatg caagtctatt
    19381 ttcatccagc aagccgctct atggaagtcc tattgcagaa tttactcaat cgtgccaaac
    19441 tactatatac tagtgaacag gagttttttg cacgaacttc acctcggcta ttaccttttt
    19501 ttgagcatcg tatccgtcta accgactacc tcagtcttga tgatggagtt atgaatactt
    19561 actttcaatc atggatagat ggaccagatc gtatattatc tgacttagcc cagcgctata
    19621 tcaataggaa agtgctcaaa tccatcactt tcaaggccga agaagaggaa gctttggacc
    19681 gcctgcgtag cctggtcgca gacgtcgggt ttgacccaga atactatact gctattcacc
    19741 acaactttga tttgccgtat gacatttacc gaccgaatgc agaaaagaaa cggactcaaa
    19801 ttgagattta tcgcaaagat gaaaccttgg tagaactatc ctctctgtca ccaattgtcc
    19861 attccttatc aggcacaatc catggcgata gccgcttcta tttcccaaaa gaaatgttag
    19921 aagaaacagg aattttcgca cctcaaatag ctgattttaa cagccacata cataacgatc
    19981 attttataat tggagaatca aatgagcatt aaacttgtcg ccatcgatat tgatggtaca
    20041 ctactaaata gtcaacacca aattacagct gaagtacact cagcaatcca agaatcaaaa
    20101 aaagcaggcg ttaaaattgt tattgctact ggtcgcccca tttccggtgt tcaagcaatt
    20161 ttagaagaac tcaatcttac agaagcaggt gactacgtca tcacttttaa cggtggctta
    20221 gtccaagatg cagcaactgg tgaggatatt gtcaaagata ccttgactta tgaggactat
    20281 ctcgacatcg aatctgctgc ccgtaaactc aagctcccaa tgcatgccag caccaaggaa
    20341 ggcatctata ctgctaaccg taacatcggt aaatacacca tttacgaatc aacactagta
    20401 agtgcaccca ttttctatcg cactcctgaa gaaatggctg acaaggaaat catcaaaaca
    20461 atgatggttg atgagccaga aattcttgat gctgctattc cacttctacc gaccagcttg
    20521 actgagaagt acaacgtagc aaaatcagca ccattttatt tggaaatcac ccctaaaact
    20581 gttaacaagg gacaagctat cattcatcta gctgagaagc ttggactaac tatggatcaa
    20641 actatggcca tcggtgacca agaaaatgac cgtccaatgt tggaagttgt tggtaatcct
    20701 gttgtcatgg aaaacggaaa tcctgaattg aagaaaatcg ccaaatacat tacgaagtca
    20761 aatgatgaga gcggtgtagc ccacgctctt agagaatggg tactgaaata gcctcttaaa
    20821 atatcaaaaa aagcatcgaa ttcaaaatcc gatgcttttt gttattagaa gttcaacaaa
    20881 gccaagaagc tttctgcttc aagtgatgca ccaccaacaa gcgcaccgtc aacgtctgga
    20941 catgccatgt attcagcaac gtttgatggg tttacagaac caccgtattg aacgcgaact
    21001 ttgtctgcaa cttcttgacc gaaatcagca gcaacaacgt cacggacagc tttacacatg
    21061 ttttgtgcat catctttagt agctgattta cctgtaccga ttgcccagat tggctcataa
    21121 gcaataacaa gtgatgctac ttgttcagca gtcaagtctt tcaaagctgc agaaacttgt
    21181 gcacctacaa attctactgc tttaccagct tcgtaagttt caagtgactc accacaacag
    21241 atgattggag tcaaaccgtt acggaagatt gcatgagctt ttttattaat atcttcatca
    21301 gtttcgtgga agtaatcacg acgctctgag tgaccgataa caacatagtt aacacccatt
    21361 tcagccaata cttttggaga tgtttcacca gtgaaggcac cagcatcttc aaagtagcag
    21421 ttttgagcag caactttcaa ttcagaatct ttagcgaacc acaagagagc gttcaaatct
    21481 acagctggag ctgcgatagc tgcttcaatt ttgtcgcctg caggcaattt tcctgcaatt
    21541 gcctcaacga aagcttgtgc ttcttgaggg tttttattca ttttccagtt accggcaatg
    21601 attggtttac gtgacatttc acttacctct tctatattta ttgtacaagg tacattatag
    21661 cataaactta gctaagatta aagaatttct gtgcatttta tgaatactta cagaaaaact
    21721 ctctacccga aagtagagag ttacaattaa gttgtctgac tataattgtt atttctggga
    21781 actcaatcga attaagcttc gatttctgta accatacctg aaccaacagt acgtccacct
    21841 tcacggatag agaaagtagt accttgttca acggcgattg ggtggatcaa ttcaacgtcg
    21901 atagtaacgt tatcaccagg cattaccatt tcagtacctt ctggcaattt gattgaacca
    21961 gttacgtcag ttgtacggaa gtagaactgt ggacggtagt tgtcgaagaa tggagtgtga
    22021 cgtccacctt cttctttagt aaggatgtaa acttcacctt tgaatttagt gtgtgggttg
    22081 atagaacctg gtttagagat aacttgacca cgttcgattt catcacgttg tacaccacga
    22141 agaagcacac caacgttatc gccggcaaga ccttcgtcaa gttgtttacg gaacatttca
    22201 acaccagtaa caactgcttt agatttttct tcttgaagac caacgatttc gatttcgtcg
    22261 ttgacacgaa cagtaccacg gtcgatacgt cctgaagcta cagtaccacg accagtgatt
    22321 gagaatacgt cctcgactgg aagcaacaat ggtttgtcag tgtcgcgttc tggttctgga
    22381 atgtactcat caacagtgtt catcaattcc ataacgatgt cttcgtactt agagtcacct
    22441 tcaagagctt taagagctga accttggata actggaagat catcacctgg gaaatcgtat
    22501 tctgaaagaa ggtcacggat ttccatttca accaactcaa gcaattcttc atcgtcaacc
    22561 aagtcaactt tgttcatgaa gacgataagg tgtttaacac caacctgacg tgaaagaagg
    22621 atgtgctcac gagtttgtgg cattggaccg tcagttgaag ctactacaag gatcgcaccg
    22681 tccatctgag cagcaccagt gatcatgttt ttaacgtagt ccgcgtgtcc tggagcgtcg
    22741 atgtgagcat agtgacgttt ttcagtttcg tactcaacgt gtgcagtgtt gatagtgata
    22801 ccgcgctcgc gctcttctgg agcagcatcg atagacgcat agtctttagg ttggttaact
    22861 gatgaaggca agcgacgtgc caatacagtt gtgatagctg ctgtcaaagt agttttaccg
    22921 tggtcaacgt gtccaattgt accaatgtta acgtgtggtt tactacgatc gtatttttct
    22981 tttgccattt tggtaaaagc ctccaataaa atatatttta tagatagaca gtaggcaata
    23041 cagtctaact ttaccttact attctatcaa attgtagcag aattgcaagc attttctatc
    23101 gttttttatt ttatccattt tctgcaaggg ctattagctt cgcttataac tttttttatc
    23161 atcccatttt aacctattac tcaactaaaa acacctcact gccataggaa tattgatgtt
    23221 accatagcta caaggtgcca caaatacgag actactgcga cagacctttc tccattcagt
    23281 catcctacga ctccctgaca agcctcttgc tataggtatt gaaaatatat tgactatttt
    23341 cacttaatga tgcagttgat aataaggctg aataggaatg gacgcgccaa tactagaaga
    23401 ataaatccag tccttgcctt ccaactgagc aaagtcttct gcttttcctg ttaggataat
    23461 gcctgcgaac tccaactgat caatctcttc ttgtttggta aagtcagtgc caccaaactt
    23521 atcgacaaag gcctgcaagt catccacagg tatgtccaga tactgtgctc gctcctgatc
    23581 tcccgattct aagaactctt tcataaagtc catacctgtc atatagtctc caggcatatc
    23641 cgaaaagact agttgaatat cctctggatc taaccccaaa agctgctctg gacgatagta
    23701 atgggtattc tgcttccctt ctgtccctat ccaataccaa ttttgcttga gattgctggg
    23761 aatcatttgc ttgatttctg ccaaactgta cttcttatca aaggaaatag ccacttctac
    23821 tagctgccca ctcatgtctt taagataatg caattcattg gcaatgtctt gagcactcca
    23881 aacagacttt tttctattgt aaaaaacagg taccttggta ttggttgtga aatcatatag
    23941 atatttgaag gtattcccag gtatatgctg agcaaaatca tagtgacgat caaacaggtc
    24001 atagtaaatt tcgtgctgac caaaagccat aggaacacca gctagatcct tatataaatt
    24061 gcagtaaagt ttcccagaaa cataactcgt gctttggatt ttaataccgt catgttttat
    24121 atttggataa gagatttcca tcaaaataat ttgtttccaa ttctcacgac cttctcgtgc
    24181 accgacgatg atttttactg tttgaacgcc aataagcatt aagatgaagc cgatgagcga
    24241 tgatagccct attgtcttcc ataaattttt ccgcttactc ttttttgtca cctcttcaaa
    24301 agtcttcata gtcatatcct ctctcttcta gtattttcct caactgcttg cgtccgcggt
    24361 aaaggataat tttcacccaa ctttgaccag tccctaagat atgggcaact tctttgacac
    24421 tcatatcttg aaaataatag aggtcaatca cttgctggta tttttctggc aggtctgcaa
    24481 ctgcttgata gagttcctca taaccttctt ggtcatagac caaaaggtct tctcttctaa
    24541 aaaactcctt ttgtaaaatt tcatggtagc gcttgtctcg cctgtatttg tcaatgtaag
    24601 cccgaactgc agatcggtaa agccaagctc ttagtttttc gaaaggaagg acaatatcag
    24661 attcaagcaa tttgactaga acatcttggg caatatcctg actgtcagct gggccggctc
    24721 ctgatttttg caaatagtag gaaatctcct ctgcaatacc aataatttct ttttcatatt
    24781 gatctagttt gatacttatc tcctcctttc actgataaaa cgaataaggg gtgatttggt
    24841 tacgttttct tataattatt atatattttt agaatagaaa aaaacaacct ccgaagaagt
    24901 tgttttatgc ctattaacct gagttgcgca aacctgtcgc cacaccattg atggtaatat
    24961 gaatgagatt ctcttgttct ttgctcaatt ctccacgacg aagacgatga attaattcaa
    25021 tctgaatata gttgagcaca ttgaaataag gcatgcgata gtcaagacta gcttttagaa
    25081 acggtagttc tgctaaaagc tcatcatttt gctcgattga caaaataatt tctttggtca
    25141 actgccattc atctaaaatg acatggaaga tatttcgtac ctcttcgctt tcacacattt
    25201 tagcatattc aaatgcgata ttcatattgg atttagacaa taccatatcc acgtttgata
    25261 gaagtgaacg gaagaatggc caagattcgt acattttttg taacttagac aaattatcag
    25321 ggtgcttatc aataaagcgt ttgaaacttg aaccaactcc ataccaacct ggcaacatga
    25381 cacggttttg tgaccaagag aatacccatg gaatcgcacg caaaccaccg atttctgtga
    25441 tggtttttcg agccgctgga cgtgagccaa tgttcagact tgatacttca cgaattggac
    25501 tagcggcaaa gaaataatca taaaaatgtg gattattgaa cacaagatct ctataaatac
    25561 gatagctatc tgatacaatt tcatccatgg tttcgcgata gccatcgatt tcatttggat
    25621 cggtaatcat ttgtgtgacc atacggtcaa gcgttgcaga taccagcatt tctaaattat
    25681 agtaggctgc gtctttgtta ccgtatttat tacctatcac ttcgccttgt tctgtcaaac
    25741 gaagccggtc cttgatggaa ccaaatggct gagatgtaat cgcatcatag gaaggacccc
    25801 caccacgacc aacagtacca ccacgaccgt ggaagaaggt gatgtttact ccgtagtctt
    25861 gaccaatttg cgtcaattca ttctgtgctt tgtagagtgt ccagccggag gataggtagc
    25921 caccatcctt gttggaatct gagtagccaa gcatgatttc ttggtagtat ttattgcctg
    25981 caatccaacg tttcgctaaa tctaattgca gatattgacg catggtatca ggtgcattat
    26041 ctaagtcctc gattgtctca aaaagtggaa caatctgaac acgggcctta tcgacatcta
    26101 ctagaccaac ttcttttaat aggacagcta attccagcaa atcagaaaca ctctccgagt
    26161 gtgaaataat atgttgcttg atgatttcct cacctagctt atctttcaat tcacgcgcag
    26221 cagcaaagat ggccaattct ttttctaatt gttctgactt tggcatgtgc gttgcagaaa
    26281 gaatgcgtgg atcagtttcc aactgtttca aaagaacatg gcatttagct tcctcagata
    26341 aactggagta gtcttctacg atacgggcag aggccaatag ctccgcaaca cttgcttcat
    26401 gaatagaaga gtcttggcgc atatcgatac tagctaggta gaaaccaaac gcttcaaccg
    26461 cttcaagcaa ttctgtaaaa tcacctttca atagcgccat agatttattt tcaaccaaag
    26521 attgtttgat ggctaataag tcagctttaa actcggctac attactatag cgaacatctg
    26581 acggcttatt gtgaactaga taatctcttg tatttgcaag tttcatctga atatagtgaa
    26641 aagcacgacg ataaggctca ttttcacggt agacagaact atcttcagat aaatccgcca
    26701 gcatcttaac cgctgtagat acatttacaa tggaagttga gagtgaaaaa ttccgataga
    26761 gtttatagag ttgatcatcg taataattca taatgacttc acactgagtc aaggcggaca
    26821 gcttcaaggt ctctgcagta acaaatggat ttccatcacg gtctccccca atccacatac
    26881 ccattgtaat tggacgagga ttatttaaga cgattccttt ctcagcggcc aaacgtttgt
    26941 attcccgctg caacttagtg acagccttaa taaaggaaga attataatac tccataacgt
    27001 ttgtgatttc gttggttacc ttcaacttct tctcacgaat catatccgtc tgcatgataa
    27061 tttcaatgta acaacggagc tcatcttccc atttgttttt attgagcaag cccaatttta
    27121 catcgcgata acgacgaagc aactcatgaa tatgcttggt taaatcaagc atagactggc
    27181 gttgaacctg agtaggatgg gctgtcaaga ctggaacaac attcaacttt tcaaggattt
    27241 cagctgcatt ttcttgtttg gaaaccatat caattgttgt tgaaatctta ccaagataat
    27301 cttgatcaat attgttttga tggttgattt cataggccaa gtcaacatct tctgatatat
    27361 taatcaaaag tggcaaaaca gcaaagtatc gagaaataac cgctagttca tcattattca
    27421 actgactgat aatagcaatc aatgcctgat aatcatcttg acgagacaat tgagataatt
    27481 ctacaatttt agcaaaggtc tctgcaggca tcatttgggc tgctactttt tccaaaatag
    27541 atgttagaat cgttacttct tcacggattg cttccttgtt gttatagttt tctaattttt
    27601 gaatagccat ctcctacctc tttcctatct ctaaattagc gtacgctcta tttcttcttc
    27661 aattaatcga cgtttttcac tagcatcaat attcagaaca aaccctatcc cgatggaaat
    27721 aatcaaaaga ctcgatccac cctgtgacaa gaaggggaaa gttacccccg ttgcaggaat
    27781 aattcctgaa attcctccga tattgacaaa tgtttgaatt agaagcatac ctgccattcc
    27841 aagtgccatc atcgcattaa atggactacg agcacggacg cctacaataa taattcgaat
    27901 aatcaaaaag aacaataagg ctagaattaa actagctccg acaaaaccga gttcttcaat
    27961 gacaatcgaa aacgcaaagt cggtgtgggc ctctggtaga tgcccctttt tctcaatcga
    28021 atttcccaag ccacgtccaa gccatccacc gttactcatc gcatagtaag aatgagtcaa
    28081 ttgaagacct gagttggtgg cattaccaaa tggatcaaaa taggccgcga aacgacgagc
    28141 aatataacca aagagtggaa ttttttccac cgtttctaca cccaaaatac gaatcgtccc
    28201 tagaataact gttgaggcgg ccacaattcc caaaaccgct gttgaaaacc agcgataggc
    28261 aatcccactc acactaatca tcacgaccat aatcagcaca ataatcgtcg catttcccaa
    28321 gtctggcagt gtcgcaacta gaccaaggag gagcagactg accacccgcc aatctgtaaa
    28381 ctctttcgga atccaacttc ctttaatcaa cgatacatag tcataagtcc gaatatccgc
    28441 ctgttgtttt gcaaactcat gtgccagata ccaaatgata attaatttca aatattctgc
    28501 cggctgcaag gttcctgcga ttggcagagg aatccagcca tgagctccgt taatcggagg
    28561 aaacaaccga gaaatagcta acagaaatac ttctaccaaa ataaccgaat agataacggc
    28621 tttcttgcgt aaaaaactta atttcatacg ataaatcgta tagattgcta ccaagctgac
    28681 aatccagaat cctgcctggt taatcactgt tctaaatgga tttccaccat taataatttg
    28741 cgtagcactg gttgtcgaat agaccatgac caacccaatc accgataaga tgagataggg
    28801 aatcagtatg gaataattca acaactggcg tttgtcaatc ttcattgctt atccaacctt
    28861 cttcgagtat agctcttatt atagcatact ttgtataaaa atacttatca ctttctaagt
    28921 tgctctgcaa acattcggaa ctgacttttg tcgacaagaa aaaggccgaa gcctttcata
    28981 tctattcaaa tgtttcaaga taaaattcaa tttcatcccc attcgcaact gtaaggtctt
    29041 tcgccccttt atcagccatt tggccattaa ctttatacat ccagtatgta ttggtactcg
    29101 catcttggct aacaccatca atttccgtta tcaaaccatt gtcttctaca atgtcatgat
    29161 gttctttaag cacatccatg acactatcac cttcttccgc agtgacttcc tgatgattcg
    29221 ctttgtcctt ggttgtaatc tgcaaagtga cttcgatagc cgattcactc tgtttctcag
    29281 tagtgtttgt acaagcagcc aagaagagcg agaagacaac catcaacata atactaattt
    29341 ttttcattta taaaatctcc taaaaatata atcaataatt ggataaaaca aaactgttga
    29401 agcagcgtgt aaactatcaa agactagacc attaagccag aagacgatag gggaagtcct
    29461 aaactgtgta gcagataaaa aacttatcgt tagcccatac aaaaagacaa gaatccccgc
    29521 cagaagactc tgtagccaca agggaatgtt ctcttttttg ataaaaggac cgattgccaa
    29581 ccaccatata aattggataa aagcaaaact aagaacttgc cacaaaacaa ctgtgccaaa
    29641 tccaaatagc aagccactcc ctaccatcac tagtatcatc aatatccagc tctccagcaa
    29701 actgaaatag gacagaacaa tgaggaaaat agccgtgaga ggttttatat tgggaaatgg
    29761 cccaaaaacc acccttaaaa caatcgtcaa agaggataga acaactagct gactaacacg
    29821 tcttactttt tcacttttgt catgcatacc aatttagcct accttattcc ctttattatg
    29881 tcaagttcag ataatctttt actatttctt gaatcgtatt tttttgattt tttcatgatt
    29941 ttacggtaga attgaggaga tgagaaagaa actaaatccg atgattgttg ttggtttttt
    30001 cctatccttc tttgctctta ttttcatcac tggggtgact ggaaatactg taaataagac
    30061 ggaggtagct aacgaacccg cagtcacaca atcaaacacc agtacaagtt ctaaaacaac
    30121 aaattctagc tcaagttatt acattgcaaa tgcgctcgaa atgaaaccta tcgtcgacgt
    30181 ttctgcttgg cagagaccgt ctgaaattga ctacgatacc cttagcaaaa acatttctgg
    30241 cgcaatcgtt cgtattcaaa gtggatcaca cactaaaaac gaaaatactg cgactgataa
    30301 aaatgggtta gataaatcct ttgataccca tattaaagaa ttccaagcaa gaaatatacc
    30361 tgttgctgtc tatgcctatg tcacgggaaa tagtattgag aacatgcgag aggaagctcg
    30421 aagcttttat aaggccgcaa ataaatacaa gccaaccttc tattggttag acgttgaaga
    30481 atataccatg gatgatatga atgctggagt ggaggctttt cgaaaagagt tagaaactct
    30541 cggcgctaaa aacattggaa tttacgtcgg aacctatttc atggaagacc acagcatcaa
    30601 tgtggataaa tttgacgcca tttggattcc tacatacggt gatgattctg gctactacaa
    30661 tgccgctcca aatactgact tgaactacga tcttcaccaa tatacctcac gtggttatgt
    30721 aaacggcttt gagcaccacc tagatctcaa cattattact accctgaagg atcctaatga
    30781 agtttatcag aaactattta caacacccga ataaaatgct acaatctcac taattacata
    30841 tacacaaagg agtgcttcgg ctgagatcgc aactgcgaaa tcctcgaacc tgatctagtt
    30901 aaaactagcg taggaattgt gtgctttata gaatgatact atactctgct ataaacactc
    30961 ctttgttgac catctacaaa ggagtctttt tatgtccaca tcaaaattac ccatcctagc
    31021 cgaagtcgct atcttttctg caattgccct cgtctttgac aaaatcccgc tctttacaat
    31081 gccccaaggt ggatctgtgt ctttagtcat gcttccaatc ttactgcttg ctctgcgcca
    31141 cggactaggg gtgggggttc ttactggtgg tattgtcgga accattcaac ttttctacgg
    31201 tggctacttc ctaaacgttt ttcaagtttt tcttgactac gcactttctt acgccggaat
    31261 tggtttagca ggtttagtgg cacccaccct atccaaacaa aaagatctta aaaatgctac
    31321 tctcatcatt actctggcta gttttctagg aggaagcatc cgattgttag ccaccttctt
    31381 atcaggaatt attttctacg cagactacgc tccagatggt atgcctgtct ggttctattc
    31441 ctttacctac aatatcagct atattcttcc ttccactgca attgcctcta ttctgctcat
    31501 tttactctat agagcaagac caggttttta caatctttaa ccaaaactgc ctgctggatt
    31561 ttatctaaca ggcagtcttt ttaattattt ttacttgctt tatgctataa taggaacgtt
    31621 ataatacatt ttggaggaaa ttatggcttc ttggatttca agaattatta aaggtatgat
    31681 tatcgcactc ggctttatct tacctggtgt ctctgggggg gtactcgctg ctatcctcgg
    31741 aatctatgag cgactaattg gatttttagc aaatattcga aaagatttca aggaaaactt
    31801 tctttatttt gttcctgtag gaattggtgg aattttggga atcgcacttt tctctttccc
    31861 tgtagaattt ttattgcaac atttccaagt cccagttttg tgggcatttg cgggagcaat
    31921 cgtgggaact attcctagcc tacttaaaga atcaactcaa aagagcaaac gagatagcat
    31981 tgatttggct tggttgattg ggacattcat tatttctggc ctcctacttt acaatttgag
    32041 cgatcttgtc gggacacttc cagctacttt tgctagtttt gtattggcag gtgccttgat
    32101 cgctttaggg gtacttgttc ctggactcag cccttcaaac ttactattga ttttgggtat
    32161 ctacacgcca atgctaaatg gtttcaaatc acttgatcta ttcggtacct tcctaccaat
    32221 cgcaatcggt ggtgttcttg caatggttgc attttcaaaa gcaatggacc acgctctgaa
    32281 agtataccat tcacgtgttt atcactttat tattggtatc gtttcatctt ctactctcct
    32341 aattttgatt ccacaagcaa gtaataaaga gtcaatttcc tatgctggag cgaactttgt
    32401 tacatggatt gctgctattg tcctatttat actaggagca tggttagggc tttggatgag
    32461 taaacttgag gaaaaatata aataatggca aaaaaggtta aaataaagaa aaccttggtg
    32521 gaccaaatat tggacaaggc taagatcaat catgatagcc ttgttttaaa tgccttcgaa
    32581 ggtcaacttc caccaggaat tgaagaacac gaaatttaca aaacactggc attaactgga
    32641 gataaaactg gaccaattat cggcattgtc ccaatcacag aacacctgtc tgagaaaaaa
    32701 ttagccaaga tctctggcaa taaaaaagtg agtatgattc cgcaaaagga cttggaaaag
    32761 acaactggct acatccatgg tgccaacaat cccgtaggca ttcgtcagaa gcataatttc
    32821 ccaatctaca ttgatcaatc tgcccttgaa ctcggtcact tgattgtctc tgctggagaa
    32881 attggtcggt cgattcgaat tgacagtcag gtattagctg attttgtaaa agctgacttt
    32941 gccgatttga tcgaggggag ggactagact tgaaaatcta ctttgtccgt cacggtaaga
    33001 ccgaatggaa tttggaggga cgctttcaag gctattctgg agattcagct ctcttgcctg
    33061 aatcttatca ggatttagaa aaactaggca aataccttgc tgaaatcccc tttgatgcta
    33121 tttattctag tgatttgcag cgcgcacata gcactgccgt agaaattgcc aaagccaatc
    33181 accattgcca aaccgttcta accacgcaac aactacgtga atggaatttt ggaacgctag
    33241 aaggaagcaa gatggcaatt tttcgtgcca tctaccccaa acaagcctgg gcactcaaac
    33301 ataatctagc actttttaac aatgacttat ttgaagctga gagcgttcgc caggtaactc
    33361 aacgaatggt ggactttgtc caatcgctga aagggcaaga catggaaaca gtcctcatcg
    33421 tcagccatgg tgcctttcta accgcttcta tccatcgcct gcttggtttt ccaccagccc
    33481 aactgcgtca ccgaggtggt ttggataatg catcggttac tattttagaa acaagcgatt
    33541 tcgaaaactt tacagaatta gcttggaatg ataccagcta taaagcaaaa cagtagaatt
    33601 ttctgctgtt ttttcctatc ctattagcaa tttatactga ttttttctct cttctatgat
    33661 aaaatagaag taacagactt tggaggaata aaatttttat gtcaactgaa cattttgaag
    33721 aattaaatga ccaacagatt gtccgtcgtg agaaaatgac ggctcttgct gaacaaggca
    33781 ttgacccatt tggaaaacgt tttgagcgtt ctgccaactc agctgaattg aaggcccagt
    33841 acgaagacaa atcaaaagaa gacttggaag aattagggca aacagcaatc attgcaggtc
    33901 gtattatgac caagcgtggt aagggtaagg caggttttgc tcacattcaa gaccgcgaag
    33961 gccagattca aatctacgtt cgtaaggatg atgttggcga agaaaactat gaaatcttca
    34021 aaaaagcaga ccttggtgac tttatcggtg tcgagggaga tgtcttcaag acaaacgttg
    34081 gtgagttgtc tatccatgct cgcaagttga ctcacttgtc taaagcgctc cgcccattgc
    34141 cagaaaaatt ccacggtttg acagacatcg agacccgcta ccgcaaacgt tatttggact
    34201 tgattactaa ccgcgagagc tttgatcgct ttgtgacccg ctcaaaaatt atctcagaaa
    34261 tccgccgtta ccttgacgga cttggtttct tggaagtgga aacacctgtc cttcacaatg
    34321 aagctggtgg tgctgcagct cgtccattca tcacccacca caatgcccaa aacattgaca
    34381 tggtccttcg tatcgcgaca gaactccacc tcaaacgctt gattgtcggt ggtatggaac
    34441 gcgtttatga aatcggacgt atcttccgta acgagggtat ggatgcgact cacaaccctg
    34501 agttcacgtc aatcgaggtc taccaagctt atgcagatta cttggacatc atggacttga
    34561 ccgaaggtat tatacagcac actgccaagg ctgttgtcgg cgatggtcct gttacttatc
    34621 aaggcactga aattgctatc cacgagccat tcaagcgtat ccacatggtt gatgccatta
    34681 aggaacaaac tggcgtggac ttctggcaag agatgagctt cgaggaagca aaagcccttg
    34741 cagctgagca caaggtccct gtggaaaaac accatacaga agttggtcaa atcatcaaca
    34801 gcttctttga agaatacgtt gaagcaactc ttatccaacc aaccttcgtc tatggtcacc
    34861 cagtagccgt atccccactg gctaagaaaa acgatgaaga cccacgcttc acagatcgct
    34921 tcgagctctt catcatgacc aaggaatacg gaaatgcctt tacagaattg aacgacccaa
    34981 tcgaccaatt ggaacgcttc gaagcccaag ccaaagctaa agaactcggt gacgacgaag
    35041 cgacaggcgt tgactacgac tacatcgaag ccctcgaata cggtatgcca ccaacaggtg
    35101 gacttggaat cggtatcgac cgcctctgca tgctcttaac agacacaaca actattcggg
    35161 atgtactcct atttccgact atgaagtaag taagagtagc ttgctaggat tgttttcact
    35221 agaatgtacc aatcgctctc cagtttctta gcgattggct gaaaaggtct actagacctt
    35281 ttcactcccg acgatgaaat aaaaattaca aactgtttaa caccgttact taaaacactc
    35341 ttggaaattc caagagtgtt tttgcatgca aaaaaatccc aaccacatag tcaggatt
//
LOCUS       DASFSW010000007        28633 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.007, whole genome shotgun sequence.
ACCESSION   DASFSW010000007 DASFSW010000000
VERSION     DASFSW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28633
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.007"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1584)
                     /locus_tag="U1099_000283"
     CDS             complement(<1..1584)
                     /locus_tag="U1099_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEM4866633.1"
                     /translation="MKQIKLKATKQFILVMVMTVLLAMGRALLLSDVSFPLWFIPLPL
                     IAYLIFTFYVLVLLDKYEKFIKTKTFAKYVSYFLGFLYLVNLVYRLNAKKYQPWNIFR
                     NNLFQFELLLMLALPVLLAFLWRKKTGIREKLSQWSANHLVPDGYLLLTSLLSLSPLA
                     ISYWKDSHYESLVEKGSYIEFFSQTPLFAPIHFIGTYIFLRYLHKAFVEFKANRTNVH
                     SMLFISLALATLSHIGYQASMAGATGSYFTRHLFPGAIVFQIACLFFLNVIISLVINR
                     QILSVAVIASLNVILVTANFLKFRYRSEPLTPNDFKWVGNLGMILSFISLRVILVSLV
                     FIVLFVFIYRHIHKKYFRGQIVAAIWKRLAGISVIVSLILGMGWAIRNEKDHKIAGWI
                     PILSQVNNWRNVDWKGYAFVARYRTLSFLWLQQLSKTSMEMPENYSEKTMKAIVKKYT
                     ALAEEINAERTGQLTDQTVIYILSESLADPRRIPGVTLSQNVLPNIEYIMSQTTSGLM
                     KSDHYGGGTANIEFQVYSGL"
     gene            complement(1597..3327)
                     /locus_tag="U1099_000284"
     CDS             complement(1597..3327)
                     /locus_tag="U1099_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866634.1"
                     /translation="MLKKLLKKHPDIVAVTVFFLFSLTLLLPQILSKAVVTGDDIVFH
                     YNRFYDTAMQIKSGDFSYIMSLHGYQQSGRIVNALYGPYLAYLQGALLLLAGTWYRYQ
                     LLSNLLLGVLSATSLYFLMREVKVRYSLSVGLSMFFVTTYSVQYWWVAQGFSSWGVAL
                     FPLCLIPAVRFLKTGKVPIFLMAGAVGLMLQIHMLSALFLILAYAILFSIGWLKSQDK
                     WNVMKDVLFSVGVFLILTVNIWLPLLYVNATNELAAPFINKKFIQSTVTWSKAYFLYF
                     PYSLPIIFATFLYFMLKKWKKQSVILRGLTVTFLVFFIFSTNLFPWQLFAGKGIGLVD
                     LIQFPFRFFLYANALLLTIVGLQLTDYEVRKKLFLGLTILSTIIGVGFTWDQYNQAVN
                     DQYNKAEFLQDMLHTTLYGTTEELRASLHSKNLGEFVQLAQKSTPDYVPVLSEKEENV
                     RAVNEAKSRYDRYRDQIILPNQQFNKQVFGDSLKISWSANQSEEIQIPIVIYRDSELT
                     LNGKRLGKMDYYLSTIGVPTVTSQKGKNTLILSYKQQWWLLPVIFISLTGWILWSIYY
                     TFIYRKSNIS"
     gene            complement(3690..4598)
                     /gene="rsmA"
                     /locus_tag="U1099_000285"
     CDS             complement(3690..4598)
                     /gene="rsmA"
                     /locus_tag="U1099_000285"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEM4866635.1"
                     /translation="MKWRNKKQERTHMRIADKNVTRAVLERHGFTFKKSFGQNFLTDT
                     NILQKIVNTAEIDQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLGR
                     FDNVTVVNQDILKTDLQTQIQNFKNPDLPIKVVANLPYYITTPILMHLIESKIPFSEF
                     VVMMQREVADRISAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKM
                     VRREQPLVSVKDEDFFFRVSKASFVHRRKTLWNNLTNHFGKSDEVKDKLTRALGMAEL
                     EASVRGEALSMADFARLSDSLQEVGL"
     gene            complement(4582..5112)
                     /locus_tag="U1099_000286"
     CDS             complement(4582..5112)
                     /locus_tag="U1099_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEM4866636.1"
                     /translation="MKKVVLLRGVTPVGKNKIPKMSYLAEILTEAGLTSVQTYIQSGN
                     VVCETDILDEELSRLIHQTIKEKIGAELAIIVKHQMELTQAVAENSFGEGYDPSRVHL
                     VFTNDEINGEKLNKLLHQDFGDEELRLGSQCLYMYLPRDARKKKLNTNYLEKQLDITA
                     TMRKLSVISRLSEMAK"
     gene            complement(5126..5383)
                     /locus_tag="U1099_000287"
     CDS             complement(5126..5383)
                     /locus_tag="U1099_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEM4866637.1"
                     /translation="MGIHFTDDAWEDYLYWQGQDKKTLKRINKIIKDMQRHPFEGIAK
                     PEPLKYDYQGAWSRRIDAENRLIYMVEADRLCILSLKDHYR"
     gene            complement(5385..5645)
                     /locus_tag="U1099_000288"
     CDS             complement(5385..5645)
                     /locus_tag="U1099_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM4866638.1"
                     /translation="MEAIVYSHFRNHLKDYMKKVNDEFEPLVVVNKNPEEDIVVLSKS
                     EWDSLQETLAVARNTYLSQKVLRGMAKVKTGQTQERNLIEAD"
     gene            complement(5721..6176)
                     /locus_tag="U1099_000289"
     CDS             complement(5721..6176)
                     /locus_tag="U1099_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEM4866639.1"
                     /translation="MSRREQVVLTNMCMITDGRRVLVQDRKSEKWPGVTFPGGHVEHG
                     ESIVSSVVREVKEETGLTVSNLELCGIQNWTDPTDHYRYLVFCYKTSHFSGSIQSSDE
                     GEVFWSDRAELKNVQLADGFETMLEIFEQPQLTENFYWFEDGEWKVENR"
     gene            complement(6180..6548)
                     /locus_tag="U1099_000290"
     CDS             complement(6180..6548)
                     /locus_tag="U1099_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866640.1"
                     /translation="MDRVYAVKCLVKNEFEPTYENYSSYEEVTYLLPANVLVWDDEQV
                     DKNKLADYLRGLVEDGYVNADGIWKQSRLFHIAGVQEMDDRVEFDGPAVEVYRWFYNF
                     ERPVTEEEFLNLYYFDDLGR"
     gene            complement(6541..7110)
                     /gene="rnmV"
                     /locus_tag="U1099_000291"
     CDS             complement(6541..7110)
                     /gene="rnmV"
                     /locus_tag="U1099_000291"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEM4866641.1"
                     /translation="MTQKIKIQEVLVVEGKDDTANLKRFYEVDTYETRGSAISDDDLE
                     RIEKLHDLRGVIVFTDPDYNGERIRKIIMQAIPTVKHAFLQRDEATPKSKTTGRSLGI
                     EHASFEDLEKALAGVRGYYDDENNFDISKSDLMRLGLLMGGESRKRREYLGEKLRIGY
                     CNGKQLLKRLELFGISLVEVEEAMESYHG"
     gene            complement(7094..7876)
                     /locus_tag="U1099_000292"
     CDS             complement(7094..7876)
                     /locus_tag="U1099_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEM4866642.1"
                     /translation="MIQIFDTHTHLNVEQFDGRVQEELDFAKEMGVTLHNVVGFDRAT
                     IDKAMELADQHPEIYLTLGWHPTEAGTYDEEVENYLLKALQHPKVIALGEIGLDYYWM
                     TADKEIQARVFKRQMELAKQLDLPFVVHTRDALEDTYQIIKEVGVGPRGGIMHSFSGT
                     LEEAQAFMDLGMHISFSGVVTFKKAVELQEAAQALPLEKILVETDAPYLAPVPKRGRE
                     NRTGYTRYVVDMLAQLRNQSVEEVARATYENARSLFRIDSEN"
     gene            complement(7984..8121)
                     /gene="rpmH"
                     /locus_tag="U1099_000293"
     CDS             complement(7984..8121)
                     /gene="rpmH"
                     /locus_tag="U1099_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEM4866643.1"
                     /translation="MKRTFQPSKIRRARKHGFRSRMATKNGRRVLAARRRKGRKVLTV
                     A"
     gene            complement(8289..9284)
                     /locus_tag="U1099_000294"
     CDS             complement(8289..9284)
                     /locus_tag="U1099_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEM4866644.1"
                     /translation="MKFTGASVEEAIQNGLVELNIPRKKAHITVVAREKKGFLGFGKK
                     PAIVDIDVINETTVVKANQKAVRGVPSEINDLNEPVKSVSEATIDLGKVVAAVKEFEK
                     SGQTLDDDIKAQILKNEKEATTILEETGRIEILSEDLLTTTDATTSAKEDVNQEVVSE
                     NQADNFADLDIKIEPQYDIEQVVSEVTAYVQEILDDMDVEARIDTSHNRRTINIQVDT
                     NEPGRVIGYHGKVLKALQLLAQNFLYNRYERNFYITINVNDYVEHRAEVLQGYAQKLA
                     ERVLAEQEAYHTDPMSSSERKIIHRIISKMDGLTSYSEGSEPNRYVVVDIEGNHD"
     gene            complement(9304..10116)
                     /locus_tag="U1099_000295"
     CDS             complement(9304..10116)
                     /locus_tag="U1099_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM4866645.1"
                     /translation="MKKKVKWAGLLVASLIVLTACGTGPVTSQSTDAWDRLIYWFASI
                     IQFLSINGQIGIGIILFTILIRTLLLPLFQIQMNSSRKMQELQPQLKKLQAEYPGTDM
                     ESRQQLYEATQALYKENGVSMRASMIPLFIQMPILLALFQALTRVEALKVGHFLWLNL
                     GEPDPFFILPIMAAVFTFLSSWLTNKSAIEKNMAMTIMTYVMPVMIFFFAVAAASGVA
                     LYWTVSNAYQVAQTLVFNNPFKIIAERQAKIDAEKERQAKIRRAQKKAQKKK"
     gene            complement(10100..10459)
                     /gene="rnpA"
                     /locus_tag="U1099_000296"
     CDS             complement(10100..10459)
                     /gene="rnpA"
                     /locus_tag="U1099_000296"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEM4866646.1"
                     /translation="MKKSYCVKRERDFNDIFTKGNNVANRRFVVYRLPKEQKHFRVGL
                     SVSKKLGNAVMRNKIKRRIRHILIECQHQLVADDFVIIARKGVEELSYQEMKQNLIHV
                     LKLAKLYQEGVVIEKES"
     gene            complement(10530..11924)
                     /gene="argH"
                     /locus_tag="U1099_000297"
     CDS             complement(10530..11924)
                     /gene="argH"
                     /locus_tag="U1099_000297"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEM4866647.1"
                     /translation="MEAKKLWGGRFEASLEEWVEEFGASIRFDQKLAKYDIQGSLAHV
                     KMLGQTGIVSQAEAQVIQAGLEELLEEYEAGKLVFDIRNEDIHMNIESLLTEKIGSVA
                     GKIHTARSRNDQVATDMHLYLKDTLFQVVDKLTNLRQILVDLAQEHVETIMPGYTHLQ
                     HAQPISFAQHLLAYYNMFSRDSERFAFNMQHTNVSPLGAAALAGTTFPIDRRMTSDLM
                     GFAKPYSNSLDAVSDRDFILEFLSNASILMMHMSRLCEEIINWCSHEYQFVTLSDTFS
                     TGSSIMPQKKNPDMAELIRGKTGRVYGNLVGLLTVMKSLPLTYNKDLQEDKEGMFDTA
                     ETVLISIDILAGMLQSMTVHKERMAQSTEKDFSNATELADYLASKGLPFRQAHEIVGK
                     LVLECSKAGHYLQDVSFETYQAISPLIQADIYDALSSKVAVSRRNSLGGTGFESIADQ
                     LKSAKEEIQNAKSL"
     gene            complement(11938..13131)
                     /locus_tag="U1099_000298"
     CDS             complement(11938..13131)
                     /locus_tag="U1099_000298"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEM4866648.1"
                     /translation="MTKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALSIGAIESHVLDVKEEFAQEYVLPALQAHAYYEQKYPLVSALSRPLISKKLVE
                     MAHKTGATTIAHGCTGKGNDQVRFEVAIAALDPSLKVVAPVREWKWAREEEIIFAKEN
                     GVPVPADLDSPYSVDQNLWGRANECGVLENPWNEAPEDAFGITTSPENAPDTPEYVDI
                     EFKAGIPVAVNGEKLSLANLIQRLNVIAGKHGVGRIDHVENRLVGIKSREIYECPGAI
                     TLLTAHKEIEDLTLVREVSHFKPIVSNELSNLIYNGLWFNPATDALKAYLAQTQAVVN
                     GTAKVKLYKGSAKVVARKSPNSLYDEDLATYTSADTFDQDAAVGFIKLWGLPTKVNAE
                     IHKKS"
     gene            complement(13324..13872)
                     /locus_tag="U1099_000299"
     CDS             complement(13324..13872)
                     /locus_tag="U1099_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866649.1"
                     /translation="MSQLTLSSKNSVKQLSISAILTAFAILIPLMMPIKIIIGPASYT
                     LASHIPLFIAMFISPATAIFVALGSSLGFFLAGFPIVIVFRALTHLFFLTLGAVLVKR
                     FPTLMDSKRFLLLGIGLNLLHGLGEYIVVMVLTSGQQTSATYWITMLGLVGVGSAIHG
                     LLDFSLAYYFWKILKERKIYQP"
     gene            complement(13952..14470)
                     /locus_tag="U1099_000300"
     CDS             complement(13952..14470)
                     /locus_tag="U1099_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEM4866650.1"
                     /translation="MKATERRKKILSLLRESPYPLAANYLAKEFGVSRQVIVGDVALL
                     RAEGEAVLATPRGYLLQSSLPSTEGAYIRKIACQHGPEEVEKELTILLEGGAQILDVE
                     IEHPIYGLLSGKLNIASLEDRDHFLDALGQYQGVLLSNLTEGVHTHTVSFPSVQAYNE
                     LSAALKHAGILL"
     gene            complement(14576..15451)
                     /locus_tag="U1099_000301"
     CDS             complement(14576..15451)
                     /locus_tag="U1099_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM4866651.1"
                     /translation="MQTIIPHLWYDTEAKEAVAFYVELFGGKIDWTYTITDTPSGDSD
                     LIQFQLGDMTLAAISAGPYFKLNESMSLMVNVASKDEVTRLYQVLSEGGRVLMPLGEC
                     PFSPYYVWLEDRFGLSWQLSYAPDLDKPYQFDICLLFSQEQVGLAQPMLDYYKDKLHQ
                     ARLGRLSYYGEGEASVAAAKLNYAELFIGDQKIIAMDHGYGGVASFNEAFSFMVYVDS
                     QEEAESWYEKVSAVPEAEICGWAKDQFGISWQIVPRILMEAYDTANPEKVKAVNAAVM
                     TMKRLDIAAIQALLN"
     gene            15572..16072
                     /locus_tag="U1099_000302"
     CDS             15572..16072
                     /locus_tag="U1099_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM4866652.1"
                     /translation="MEIWNTYTADGHLTDHTLTRGESIPDGLYHLVVECIIRHHDGST
                     LFMKRDSTKPSYPDYYEATAGGSALFGEIAEQAILREVREETGIELTADQLRHHTHFV
                     AHDDQCIFHCYWAETDWDKSTIQLQSNETSNYIWVPQENLKNFLETELVIPRQKDYVE
                     RLFLKS"
     gene            complement(16127..17581)
                     /locus_tag="U1099_000303"
     CDS             complement(16127..17581)
                     /locus_tag="U1099_000303"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEM4866653.1"
                     /translation="MTKPIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGIDWDESPETHEQYRQSERLDIYQKYVDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEDEKAAYIAEREAAGIVPTVRLAV
                     NETGIYKWNDMVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVVDDYLMKISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRRKGYM
                     PEAVFNFIGLLGWNPGGEEEIFSREQFIQLFDENRLSKSPAAFDQKKMDWMSNEYIKN
                     ADLETIFNLAKPFLEEAGRLTDKAEKLVELYKPQLSSADEIVGLTDLFFSDFPELTAE
                     EKEVMAGETVPTVLNALKEKLEAMTDEDFQPDNIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGREKSIQHIDNMLKSL"
     gene            complement(17653..18933)
                     /locus_tag="U1099_000304"
     CDS             complement(17653..18933)
                     /locus_tag="U1099_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM4866654.1"
                     /translation="MKKILKNATYLWVLTADMLSNFGDVVYYLALMNYVLLVPNSRLA
                     LAIVTFSEIFPSFMGLFTGYLADKTVNKISTIKLTLLFRVLLYLILGFCMGFEPALWI
                     VVVAATFNVFADFAGFYENGLYTPLGLRVAPKEEREQYSAFRQTVTSLLNIAFQALSA
                     LLVGLLSYQNLAFLNAGTFLAALLIMQLLTPVFRKLLTEQPLKIAEQSSQTEQKTKRP
                     GILASFKQAIVELRKIPEFRLVLITTPLINACGAILYPILVLLISEDPGLVFLNVETT
                     LALTILIFFIGHILGSTLVFTLFKNTSMVTLEVAATFSLLGVFVGMLLHQLPIIFFFL
                     TTMGISSGASGPKFNAKFVNSMPEEQLATIGGAVSTYFMLGQAFTRLLVSGLVLILTV
                     NQISGLFLAATGLLVLYILYWLARNQKTPQNQSV"
     gene            complement(19025..19684)
                     /locus_tag="U1099_000305"
     CDS             complement(19025..19684)
                     /locus_tag="U1099_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866655.1"
                     /translation="MKRLLIGAAVIGLAVLVLLRDSLGLHLAMPFWTTVWFVLTFVTG
                     FHRLVKLDWLASIFCFGLAFIAANSVYEWVEFGTWNMFLSLVLLCLGLKLIFKPRKRR
                     LAHGVVTGVIGDKGRDTAFGSSTRYIHEENFIHDSVDVAFGSSTVYFDQAVILGNKAD
                     FHIDAAFSNITLYLPADWQAVVELDSAMSTTNNYASEPKEKVLLVTGDMAFSTLNIYS
                     M"
     gene            complement(19689..20135)
                     /locus_tag="U1099_000306"
     CDS             complement(19689..20135)
                     /locus_tag="U1099_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HEM4866656.1"
                     /translation="MKVRIEFDDSLDHVEVVIRTSQLGPEIEQIQQALQQVSRPSLVF
                     YKGSSEYFLSLGDILFFETDGTKIFAHTGDDAYEVKMKLYELGEHLPIYFCRVSKSTI
                     ANARAVYSLDKSFSGTSRISFYQTHKEVHVSRHYYHLLKEKLREVR"
     gene            complement(20313..21071)
                     /locus_tag="U1099_000307"
     CDS             complement(20313..21071)
                     /locus_tag="U1099_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEM4866657.1"
                     /translation="MSDNRMKYTIDSNMQFPLVEIALEAGESAYIQQGSMVYHTPSVT
                     LNTKLNARGGSGFGKLMGAIGRSMVSGESMFITQAISNSDDGKIALAPSTPGQVIALE
                     LGAEQYRLNDGAFLALDGSAQYKMERQSVGKAFFGGQGGLFVMTTEGRGTLLANAFGS
                     IKKLTLDGGSITIDNAHVVAWSRDLDYNIHMENGFLQSIGTGEGIVNTFTGYGEIYVQ
                     SLNIETFAQVIGNHIVGAGGDGGGGSTSLLDAIF"
     gene            complement(21279..21773)
                     /locus_tag="U1099_000308"
     CDS             complement(21279..21773)
                     /locus_tag="U1099_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEM4866658.1"
                     /translation="MSYFQNFMKANKAYVDLHGDYHLPLRPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQLGTREIVVLHHTDCGAQTFTNEEF
                     AVQLKRDLGVDVAGQDFLPFIDIEESVREDIALLKQSPLIPDDVEISGAVYDVDTGRM
                     TEVI"
     gene            complement(21894..23264)
                     /gene="radA"
                     /locus_tag="U1099_000309"
     CDS             complement(21894..23264)
                     /gene="radA"
                     /locus_tag="U1099_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEM4866659.1"
                     /translation="MTIAKKKTTFVCQSCEYHSPKYLGRCPNCGSWSSFVEEVEVAEV
                     KNERVSLTGEKTRPMKLNEVSSIQVARTKTNMEEFNRVLGGGVVPGSLVLIGGDPGIG
                     KSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRTE
                     IEKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTK
                     EGTLAGPRTLEHMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVL
                     NPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRAS
                     LIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFI
                     GEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVPKEITVISVTTIGEVL
                     QKVFNL"
     gene            complement(23271..23804)
                     /locus_tag="U1099_000310"
     CDS             complement(23271..23804)
                     /locus_tag="U1099_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM4866660.1"
                     /translation="MQILFVRHSEPDYSMFDQHDNPRLYAGFGRDLAPLTEKGRTLAQ
                     EIASNPIFSQAQVVVASSVTRALETATYIAHAQQLPLLVEPFFHEWRPDMTGQNASQD
                     EAVLAHRLFLENSGAVPESSPVRYETAAEMRERFLQALEKYKAYDRIVIVCHGMLIRQ
                     FVPKETIAYCEILEYTL"
     gene            complement(23811..24254)
                     /locus_tag="U1099_000311"
     CDS             complement(23811..24254)
                     /locus_tag="U1099_000311"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEM4866661.1"
                     /translation="MKIRGFELVSQFTDENLLPKRETAHAAGYDLKAAERISLEPGEI
                     KLVPTGVKAYMQANEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPANEGHIFAQM
                     RNITEETVVVEAGERMVQAVFAPFLLADGDQADGVRTGGFGSTGK"
     gene            complement(24351..25496)
                     /locus_tag="U1099_000312"
     CDS             complement(24351..25496)
                     /locus_tag="U1099_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4866662.1"
                     /translation="MKIIEVTKKDGSIVYRASVYLGVDAITGKKVKTSVTGRTKTEVK
                     QKAKHAPIEFAKNGSTVKKEVEVKTYQELADLWLESYKLTVKAQTFVVTKRQLYNHLI
                     PIFGGMKLDKIVPATIQQFVNGLAPKLVNFKAIHSINNRILQHGVLLQVLATNPARDV
                     ILPKKQKKGREAVRFIEPEHLKQFLDHAEQLASTNFSKYYQYVIFKLLLATGCRIGEL
                     SALEWSDIDLEAGTISISKTYSMELHIIGTTKTKAGTRIISIDKKTALLLKQYRNRQR
                     LMFLEVGACAPDVVFATTVREYLPRISLQEIINRRCKALGIPRFTCHAFRHTHASLLL
                     NAGISYKELQYRLGHSNISMTLDTYGHLSKDKEKEAVSYYEKAINNL"
     gene            complement(25965..26897)
                     /locus_tag="U1099_000313"
     CDS             complement(25965..26897)
                     /locus_tag="U1099_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM4866663.1"
                     /translation="MNNNVQVGERIKQIRLSLGESMEKFGERFETSKGTVNNWEKGRN
                     LPNKENLKKISELGQMTVNELLYGNVNNLIESILLGFEFYSFADENKKKRVFELVQKE
                     VKDFPHLLTSDYNDELIEKISDIVVDIINGLDGTNESALRASYEAFSVSGIEDMFYRT
                     VTTSEIEIRYNYSLMMYQKNYKLKKILREGMDEQLYKKLEFVHKQAEAEVTKIARELD
                     YKLYNSDESKKETKKQIFDLIDKIQSIVPKPLRTKKIKKNIDVWVHNNFKELIESPES
                     LSELINTGALKKMSLGEIEPKYIDMTKLIEPDED"
     gene            27020..27226
                     /locus_tag="U1099_000314"
     CDS             27020..27226
                     /locus_tag="U1099_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4866664.1"
                     /translation="MTRKNKIRGYRNMLGLTQEQLGKKLGMTKQSYHNKEVGKNSFSD
                     DEKLKFKQLLLPLFPDITLEDIFF"
     gene            27457..27960
                     /locus_tag="U1099_000315"
     CDS             27457..27960
                     /locus_tag="U1099_000315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEM4866665.1"
                     /translation="MTPITSQGTQTDIVGIDSRTVAEWTGKQHKELMRDIRQYTEYLK
                     SADLRCTDFWQETTYTASNGKENPCYMITKKGCEFIQHKMTGQKGAVFTAKYINYFWE
                     QGQQLQEATQILLDLSDQPTQPAIELNSDMAYIKNRLSELHSMTTMADIKVGLAKTYR
                     IAELMED"
     gene            27974..28594
                     /locus_tag="U1099_000316"
     CDS             27974..28594
                     /locus_tag="U1099_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEM4866666.1"
                     /translation="MELVYMDGWKEPYTLSSIVAECAEVKHRHLKMLINKHKDRLERF
                     GKVHFKISPSKSGQNVRDYILNEQQATLLITFLRNTEQVANFKENLVRAFFEMRDEVA
                     EFRYQRALEKPKRKALHEAIETWQEAPKHAHSTVTNLLLKGTTGMNKRQLVAHRGGFN
                     GIDSLTSQELIRYQALEDMAIAMINLGMTYQEIKNMVFRKNAPQGA"
BASE COUNT         8289 a         6591 c         5773 g         7980 t
ORIGIN      
        1 cagaccacta tagacctgaa actcgatatt agccgtacca ccaccatagt gatcactctt
       61 catcaagcca ctggtagtct gactcataat gtactcaata tttggaagga cattttgact
      121 aagggttaca ccgggaatcc gacgcgggtc agccaaactc tcactaagaa tgtagataac
      181 cgtctggtca gtcaattgac ctgtccgttc tgcattgatt tcctcagcca gagcggtata
      241 cttcttcaca atggccttca tggtcttctc agagtaattc tcaggcattt ccatactggt
      301 cttgctgagt tgctgcaacc agaggaatga aagagtgcga taacgagcca caaaggcgta
      361 gcccttccag tctacgttcc gccagttatt gacctgagaa agaataggaa tccagccagc
      421 aatcttgtgg tctttttcat ttcgaatagc ccagcccatt cccaaaatta gggatacgat
      481 gactgaaatt cctgccagtc gtttccagat agcagcgaca atctgccctc ggaaatactt
      541 cttgtgaata tgtcggtaga taaacacaaa gaggactata aagacgagcg ataccaaaat
      601 aacacgtaga ctgataaagc tgagaatcat ccccaagttc ccgacccatt tgaaatcatt
      661 gggggtcaat ggctccgaac ggtagcggaa tttgagaaaa ttggccgtaa ccaaaatgac
      721 gtttagacta gcaatcacgg caacagataa gatttggcgg ttgataacta ggctaatgat
      781 gacattgagg aaaaagaggc aggcaatctg gaatacaata gcccctggaa agagatgacg
      841 tgtgaaataa gaaccagtcg ctccagccat agaagcctga taaccaatat gcgatagcgt
      901 agccagtgcc aaactgataa agagcatgct gtggacattg gttcgattgg ccttaaactc
      961 tacaaatgcc ttatgaagat agcgaagaaa aatgtaggtc cctataaaat gaataggtgc
     1021 aaacagcggt gtctggctga aaaactctat gtaagaacct ttctcaacca aactctcata
     1081 atggctatct ttccagtaag atatggctag aggactgagc gagagtaagc tggtcaagag
     1141 caaatagcca tctggtacca agtgattggc tgaccactgg cttaattttt ctcgaattcc
     1201 cgtcttcttc cgccataaaa atgccaacaa tacaggaaga gccaacatca atagaagctc
     1261 aaattgaaag agattattcc ggaaaatatt ccagggttga tacttcttgg cattgagccg
     1321 atagaccagg ttgacaagat atagaaaacc tagaaaatag ctgacatact tggcaaaggt
     1381 tttggtcttt ataaatttct catatttatc aagcaagacc aatacataaa atgtaaaaat
     1441 aagataggca atcaagggta ggggaataaa ccagagtgga aaagatacat cactcagtaa
     1501 aagggctcgc cccatagcca gcaagacggt cattaccatg accagaataa attgcttggt
     1561 tgccttgagt ttgatttgtt tcatagcatt tctctcttag gaaatatttg attttcggta
     1621 gataaaggta taataaatcg accaaagtat ccagccagtc aaactgataa agataactgg
     1681 caataaccac cattgttgct tataggagag gattagggtg ttcttccctt tttggctggt
     1741 aaccgttggc acaccaatag tggaaagata ataatccatc ttccctaacc tctttccatt
     1801 caatgtcaat tcactatctc ggtaaataac aatcggtatt tggatttcct ctgattgatt
     1861 tgctgaccaa cttattttta agctatcccc aaaaacttgc ttattaaatt gctggttagg
     1921 taaaataatc tggtcacggt aacgatcata acgagattta gcctcattta ccgcacgaac
     1981 attctcttct ttttcagata acaccggcac atagtcggga gttgatttct gagccagttg
     2041 aacaaactca cctaaatttt ttgaatgcaa ggatgcacgt aattcttccg ttgttccgta
     2101 tagagtagta tgaagcatat cttgtaagaa ttctgctttg ttgtactggt cattgactgc
     2161 ttgattatat tgatcccaag taaaaccaac accaattatg gtagataaga ttgtcaaccc
     2221 caaaaacaac tttttacgta cttcgtaatc tgttaattga agaccaacaa tcgtcagaag
     2281 taaagcatta gcatacaaaa agaatctaaa gggaaattgg attaaatcca ctaatccaat
     2341 acctttacca gcaaatagtt gccacggaaa aagatttgtt gaaaaaataa aaaataccag
     2401 aaaagttact gtcagccccc tcaatatcac cgattgcttc ttccattttt tcagcataaa
     2461 ataaagaaag gttgcaaaaa tgattggtag agagtatgga aagtataaga aataagcctt
     2521 agaccaggta actgttgact gaataaactt tttgtttata aatggtgctg ctaattcatt
     2581 tgttgcattt acatataata agggtaacca gatatttaca gttaaaatca gaaaaactcc
     2641 aaccgaaaac agtacatctt tcattacgtt ccatttgtct tgcgatttta accaaccaat
     2701 tgaaaaaaga attgcatagg ctaagatgag aaacagagcc gacagcatgt gtatctgcaa
     2761 cattaaccct acagctcctg ccatcagaaa aataggaact ttaccagttt ttaggaatcg
     2821 aactgctgga atcaagcaaa gcggaaataa ggccactccc caacttgaaa atccttgagc
     2881 tacccaccag tattgaacag agtaggtagt cacgaaaaac atggataacc caacagatag
     2941 agaatagcgt actttcactt ctcgcattag aaaataaagc gaagtggcac tcaaaactcc
     3001 taaaagaaga ttagacagta gttgatatcg ataccaagtt ccggccaaca acagcaaggc
     3061 tccttgcaaa taggctagat atggaccata tagagcattg acaatccttc cagattgctg
     3121 atagccatgc aagctcatta tataagaaaa atctcctgac ttaatttgca tagctgtatc
     3181 ataaaaacga ttataatgaa aaacaatatc gtctcctgtc accactgctt ttgacaaaat
     3241 ctgtggcaat agaagggtga gcgaaaaaag aaaaaataca gttactgcaa caatatctgg
     3301 atgctttttc agtaattttt ttaacataag ataccttcat aaaaaaagag ggaacccctc
     3361 tcttgttttt gtaagaacct gtactcagaa gtgaagtgat tatcaataag agatagtttt
     3421 tcgctaatca aggcagtttt attgagagaa tactgactgt actttgaaaa aactaacgat
     3481 gactagctta aaaactatcc ttaagtagaa gtgctgaact gtgaatacgg gttcttacta
     3541 ttctgctcat ttgcagacaa taattcctta aaatttatca actcccggta taacaagctt
     3601 tccccccact gtcaacggac aagatagatt gaaaatggtt ttaggtaaaa taaagattga
     3661 ttgcagattt ctccgcaatc aatacttgct tataaaccaa cttcctgaag gctatcagac
     3721 aaacgtgcaa agtcagccat ggaaagtgct tctccacgga cactggcttc cagctctgcc
     3781 atgccaagtg cccgcgtcag cttgtccttg acctcgtcgg acttgccaaa atggttggtc
     3841 aggttgttcc acagggtctt acggcggtgc acaaagctgg ccttggacac tcggaagaag
     3901 aaatcttcat ctttgacgct caccagaggt tgctcgcggc gaaccatttt gagaatggcg
     3961 gagtccacgt ttggagcggg cacaaagacg gtgcgaggca cgatgaaagc caccttggca
     4021 gtcatgtagt actggaccgc aatggacagg ctgccatagg ccttggtgtt tggctcagca
     4081 gaaatgcggt cggccacctc ccgctgcatc atgaccacaa actcgctgaa gggaatcttg
     4141 ctctcaatca ggtgcatgag gataggggtg gtgatgtagt agggcaggtt ggccacgacc
     4201 ttgataggca gatctggatt cttgaagttc tgaatctggg tctgcaggtc ggtcttgagg
     4261 atatcttggt tgaccacagt cacattgtca aagcgaccca aggtgtccgc caaaatcgga
     4321 atcaggcggt cgtcaatctc aaaggccatg acctcggcgg cattctccgc caaaaactca
     4381 gtcaaggctc caattccagg accgatttcg atgacattga cattctggtc aatctctgcc
     4441 gtgttcacaa tcttctgtaa aatattagta tcggtcaaaa agttctgacc aaaggatttc
     4501 ttaaaggtaa acccgtggcg ttccaggacg gcacgagtaa cgtttttgtc tgcgattctc
     4561 atgtgtgttc tttcttgttt tttatttcgc catttcactc aatcgtgaaa taacacttag
     4621 ttttctcatc gtcgctgtaa tgtccagctg tttttctaaa taattggtat ttagtttttt
     4681 ctttcgagca tctctcggaa gatacatgta tagacattga cttcccagac gcaactcctc
     4741 atcgccaaag tcttggtgca ataatttgtt taatttttca ccatttatct catcattggt
     4801 aaagaccaaa tgaacacgag aaggatcata gccctcacca aaagaatttt cagcaactgc
     4861 ttgtgttaac tccatctgat gtttgacaat gatcgctaac tctgcaccta ttttttcttt
     4921 aatggtttga tggataagcc gactgagttc ttcatctaat atatctgttt cacaaacaac
     4981 atttccgctt tgaatatagg tttggacaga agttaaacca gcttccgtca aaatttccgc
     5041 caagtaagac atcttgggaa ttttattctt gccaacaggg gtaacacctc gaaggaggac
     5101 gacttttttc atttgttaat ccccctcacc tataatgatc ctttagcgac aggatgcaca
     5161 accgatcagc ttccaccata taaatcaacc gattctccgc atcaattctc cgagaccagg
     5221 caccttgata atcatacttc aaaggttctg gtttggcaat gccttcaaaa ggatgtcgct
     5281 gcatatcctt gataatctta ttgattctct tcagtgtttt cttatcctga ccctgccaat
     5341 aaagatagtc ttcccaggcg tcgtctgtaa aatggattcc catgttagtc cgcctctatc
     5401 aggtttcgtt cttgggtttg tcccgttttg actttagcca taccacgtag gactttttga
     5461 gacagataag tgttccgagc aacagcgagg gtttcttgta aactatccca ctcactcttt
     5521 gagagtacaa caatatcttc ctctggattt ttattaacca cgaccaaagg ttcaaactcg
     5581 tcattgacct ttttcatata gtcctttaag tgatttcgaa aatgggaata tacaatagct
     5641 tccataagca atacctctca tcatttctag gcttactata gcacagaaaa aaagagttgt
     5701 caaacttatt gtacaactct ttagcgattc tccaccttcc actcgccgtc ttcaaaccag
     5761 taaaaattct ccgtcagctg tggctgttca aagatttcca gcatggtttc aaagccgtca
     5821 gccagttgaa cattcttcaa ttctgcacgg tcactccaga agacctcccc ctcatctgaa
     5881 gattgaatag aacctgaaaa atgactagtt ttatagcaaa ataccagata acgatagtgg
     5941 tcagtcggat ccgtccagtt ttgaatgcca cagagttcga gattggatac cgtcaagcct
     6001 gtttcttctt tgacctcgcg gacgacagag gagacaatac tctccccgtg ctcaacatgg
     6061 cccccaggga aagtcacacc aggccacttt tcagacttcc tatcctggac caacaccctc
     6121 cgaccgtctg taatcatgca catattggtt aagacaactt gttctcgtct agacattccc
     6181 tatctcccca aatcatcaaa ataatagaga ttcaaaaatt cttcctcagt aactggacgt
     6241 tcaaaattgt aaaaccagcg atagacttcc acggctggtc catcaaactc taccctgtcg
     6301 tccatttctt gcacaccggc aatatgaaac aaacggctct gcttccaaat accatccgca
     6361 ttgacatagc catcttcaac taaaccacgc aaatagtctg ctagtttgtt cttatcaacc
     6421 tgctcatcat cccaaacaag aacattggcc ggcagcaagt aggtcacttc ttcataagat
     6481 gagtaattct cataagtagg ctcaaactcg tttttaacca agcacttaac agcgtagacc
     6541 ctatccatga tagctctcca tagcctcctc aacctccacc aaacttatcc caaacaattc
     6601 caatcgtttg aggagttgtt tgccgttgca gtagccgatg cggagcttct ctcccagata
     6661 ctccctccgc ttgcggcttt caccccccat cagcaatcct aggcgcatta gatctgactt
     6721 gctgatgtca aagttatttt catcatcata gtagccacgc acgcctgcga gggccttttc
     6781 caagtcctca aagctggcgt gctcaatgcc caaggaccgc cctgttgttt tggacttagg
     6841 tgtagcctcg tcccgctgta agaaggcgtg tttgaccgtg ggaatggcct gcataatgat
     6901 ttttcgaatc cgctcgccat tgtagtcggg gtcagtaaag acaatgacgc cccgcaggtc
     6961 atggagtttc tcgatgcgtt ccaagtcatc gtctgatatg gcagagcctc tggtttcgta
     7021 ggtatccacc tcgtaaaatc gttttaggtt tgctgtatca tccttgccct cgacaacgag
     7081 gacttcttgt atcttaattt tctgagtcaa ttctgaacaa gctccttgca ttctcgtagg
     7141 tcgctctcgc cacctcttcg accgactggt tgcgaagctg ggccagcata tccaccacat
     7201 atctggtgta gccggtgcga ttttcccgac cacgcttggg aactggggcc aggtagggcg
     7261 catccgtctc gaccaggatt ttttctaggg gcaatgcttg cgccgcttcc tgaagttcaa
     7321 cggctttttt gaaggtcacc actcccgaga aggagatgtg catgcccagg tccatgaaag
     7381 cttgagcctc ctccagagtg ccagagaagg aatgcatgat gcctccacgc ggaccgacgc
     7441 cgacttcctt gatgatctgg taagtatctt ccaaagcatc gcgagtatgg accacaaagg
     7501 gcaaatccaa ctgcttggcc agctccatct gtcgcttgaa aacacgagcc tggatttcct
     7561 tatccgccgt catccagtag taatccagtc caatctctcc cagggcaatg accttgggat
     7621 gctggagggc tttgagcaga tagttttcca cttcctcgtc ataagtgcca gcctctgtcg
     7681 gatgccagcc caatgtcaga taaatttcag ggtgctggtc agcaagctcc atagccttgt
     7741 caatggttgc acggtcaaaa ccgaccacat tgtggagggt gacccccatc tccttggcaa
     7801 agtctaattc ttcttggacc cgaccgtcaa actgctcgac gttgaggtgg gtatgggtat
     7861 caaaaatttg tatcatcgtt ccatcacttt ctttcctact attgtaacat atttcagaag
     7921 attcttatga gaagaaaaaa aaccagcaga ctcgagtttc tgctggcttc tcttttcatc
     7981 ttcttatgct actgtaagaa ctttacgtcc cttacggcga cgagcagcta acacgcgacg
     8041 gccgttttta gttgccatac ggctacggaa accgtgttta cgcgcgcgac ggattttact
     8101 tggttgaaaa gtacgtttca cgatgaatac ctcctcttag attcttgtat tcgtttagcc
     8161 ggctagtttt gatcagttac taaacatacc aaactatttt atataaattc tgttcgtttg
     8221 tcaagctatt ctgccatact tttaggaaag aatcagaaaa tcattgaaaa aagaggacta
     8281 gcctcttttt aatcatgatt tccttcaata tctacaacga catagcggtt tggttcgcta
     8341 ccttctgaat agctcgtcaa accatccatt ttagagataa tgcggtggat aattttacgt
     8401 tcgctacttg acatcggatc tgtatggtag gcttcctgct ctgccaaaac acgttctgcc
     8461 aatttttgtg cgtagccttg aagaacctct gcgcggtgtt ctacataatc attcacattg
     8521 attgtaatat agaaattacg ctcataacgg ttgtataaga agttctgagc caaaagttgc
     8581 aaagccttta agacttttcc gtgataaccg ataacacgac ctggttcatt ggtatccact
     8641 tgaatattga ttgtacggcg attatgactt gtatcaatac gcgcctcaac atccatatca
     8701 tccagaattt cttgtacata agctgtcact tctgaaacaa cttgttcaat atcatactgg
     8761 ggctcaatct taatatctaa atcagcaaaa ttatctgctt gattttctga gacgacttct
     8821 tgatttacgt cttccttagc agatgttgtt gcatctgtag tagttagtaa atcttctgaa
     8881 agaatttcaa tccgacctgt ttcttccaaa atcgttgtcg cttctttttc atttttcaag
     8941 atctgcgctt taatatcatc atcaagcgtt tgaccagact tttcaaattc ttttaccgct
     9001 gcaacaactt ttcctaggtc aatcgtagcc tctgaaacgc tttttactgg ttcattgagg
     9061 tcattaattt cacttggaac gccacgaact gctttctgat tagccttaac aaccgttgtt
     9121 tcattgatga catcaatatc cacaatcgct ggctttttac cgaaaccaag gaaacctttt
     9181 ttctcacgcg caaccacagt aatatgtgct ttttttcgag gaatatttag ctctaccaag
     9241 ccattctgga tggcctcttc aacacttgct cctgtaaatt tcatcttctc tctcctagtt
     9301 ttcctatttc tttttctgtg cttttttctg tgcccgtcgg atttttgcct gacgttcttt
     9361 ttccgcatca attttcgctt gacgctctgc tattatttta aacggattat tgaacaccaa
     9421 ggtttgagca acctgatagg catttgacac tgtccaatac aaagccaccc cactagcagc
     9481 tgccacagca aagaaaaaga tcatcacggg catgacatag gtcataattg tcatagccat
     9541 attcttctca attgctgatt tattggtcaa ccatgagctg aggaatgtaa acactgcagc
     9601 cataatcggc aaaataaaga acggatccgg ctccccaaga tttaaccaaa ggaagtgacc
     9661 tactttcaag gcttcaacac gtgtcagagc ttgaaagagg gccaaaagga ttggcatttg
     9721 aataaagagc ggaatcatgg acgctctcat gctgacgcca ttctctttgt agagagcttg
     9781 cgttgcttca taaagctgct ggcgactttc catatccgtt cctggatact ccgcttgcaa
     9841 ctttttcagc tgaggttgaa gctcctgcat cttccgtgag gaattcatct gtatttggaa
     9901 caaaggcaac agaagggttc gaattaaaat ggtaaaaagt ataataccga ttccaatttg
     9961 accgttgata gataggaact gaataatgct ggcaaaccag taaattaatc tatcccacgc
    10021 atctgtagat tgactggtca ccggccctgt tccgcaggct gttaaaacaa ttagagatgc
    10081 taccagtaga cctgcccatt taactttctt tttcaatcac aacaccttcc tggtatagtt
    10141 tagctagttt caaaacatgg atgaggtttt gcttcatttc ctgataggac aattcctcca
    10201 caccttttcg agcaatgata acaaagtcat ctgctaccag ttgatgttgg cattcaatta
    10261 agatgtgacg aatcctgcgc ttaatcttat ttcgcatgac agcgtttccc aattttttgc
    10321 tgacagataa acctactcgg aaatgctttt gctctttagg taatcggtag acaacaaacc
    10381 tccgattggc gacattgttt ccttttgtaa aaatgtcatt aaaatctcgt tcacgcttga
    10441 cacaatagct ttttttcaaa ctgccactcc atctttcaaa attacttcta ttataccaca
    10501 aaattcaaaa aagccaataa acataccgtt tataggcttt ttgcgttctg aatttcctct
    10561 ttggcacttt tcaactggtc tgcaatcgat tcaaaacctg tcccgccaag ggaatttcta
    10621 cgactaacag caactttgga ggacaaggca tcataaatat ctgcttgaat aagaggcgaa
    10681 atagcctgat aggtctcaaa tgatacatcc tggagataat gtcctgcctt gctacactcc
    10741 aagaccagtt ttccaacaat ctcatgcgct tggcggaagg gcagaccttt actagccaaa
    10801 taatcagcca actctgtagc atttgaaaag tccttctctg tcgattgagc catcctttcc
    10861 ttatggaccg tcatagattg caacatccct gctaaaatat caattgaaat caaaactgtt
    10921 tctgccgtgt caaacatgcc ttccttgtct tcctgcaagt ccttattata ggttaaagga
    10981 agggatttca tgactgtcag taggccaact aagttcccat aaactctacc tgttttcccc
    11041 cgaatcagct cagccatatc tggatttttc ttctgaggca tgatggaaga acctgttgaa
    11101 aaggtatcgg atagggtgac aaattggtat tcatgtgaac accagttaat gatttcttca
    11161 cacaaacgcg acatgtgcat catgaggata ctggcatttg acagaaattc caagataaaa
    11221 tcacggtcag acacagcatc aagagaatta ctatagggct tggcaaatcc catcaaatca
    11281 gacgtcatcc gacggtcaat cggaaaggtc gtccctgcca aggccgccgc accaagtggg
    11341 gatacatttg tatgctgcat gttgaatgca aagcgctcac tgtctcggct gaacatgttg
    11401 taataggcta acaagtgctg agcaaaacta atgggctggg catgttgcaa gtgggtatag
    11461 ccgggcatga ttgtttccac atgctcctgt gcaaggtcta ccaaaatctg acgtaggttg
    11521 gtcaatttgt ctacaacttg aaagagggta tccttcagat agaggtgcat atccgtagcc
    11581 acttggtcgt tacgagaacg agctgtatgg attttgcctg caactgagcc tattttctcc
    11641 gtcaatagac tttcaatatt catgtgaatg tcttcgtttc gaatatcaaa taccagcttg
    11701 ccagcctcat actcctctag caattcttcc agaccagctt gaatgacctg agcctcagct
    11761 tgagagacaa tcccagtttg ccccagcatc ttgacatggg ccagtgagcc ttgaatatca
    11821 tacttggcca atttttggtc aaagcgaatg gaggctccga attcttcaac ccattcctca
    11881 agactggctt caaagcgacc tccccataat ttctttgctt ccataagctc ctactttcta
    11941 gctcttttta tgaatttccg cattgacctt ggttggtaat ccccaaagct tgatgaaacc
    12001 aactgctgca tcttgatcaa aggtatctgc actggtatag gttgccaaat cttcgtcata
    12061 aagggaattt ggagatttcc gagcaaccac tttggctgac cccttataca attttacttt
    12121 tgcggtgcca ttaacaactg cctgggtctg agcaagatag gccttcaagg catctgtcgc
    12181 agggttgaac cacaggccat tgtaaatcaa gttagagagt tcatttgaaa caattggttt
    12241 gaaatgcgat acttcccgca caagggtcaa atcttcgatt tccttgtggg ctgtcagaag
    12301 ggtaatagca cctggacact cataaatttc ccgtgacttg attccgacta aacgattttc
    12361 cacatggtca atccgaccca caccatgttt accagcaatg acattgagtc tttgaatgag
    12421 attagcaaga cttaactttt caccatttac agcaacagga ataccggctt taaactcaat
    12481 atcgacatac tcaggagtat ctggtgcatt ttctggagaa gtagtgatgc caaaagcatc
    12541 ttctggcgct tcattccatg gattttctaa aactccgcac tcattggccc gtccccaaag
    12601 gttttggtcg actgagtaag gactgtccag gtcagctggg actggtacac cattttcctt
    12661 ggcgaagatg atttcttctt cacgcgccca cttccattca cggacaggag ccacgacttt
    12721 caagcttgga tctagggctg caatggctac ctcaaaccgc acctggtcat ttcccttacc
    12781 agtacagccg tgggcgatag tggttgctcc tgtcttatga gccatttcca ccaacttttt
    12841 agaaatcaag ggacggctga gagccgatac cagcggatat ttttgctcat agtaggcatg
    12901 ggcttgtagg gctggtagga catattcttg agcgaattct tcttttacat ccaatacatg
    12961 ggactcaata gcaccgattg ataaggcctt atcatgtatg aagtcgagat ccttgccctc
    13021 tccaacatcc atacaaaccg caatgacatc atagtctttc ttcaaccaag caatcgcaac
    13081 tgaagtatca agtccaccag aataggctaa aatcaatttt tcttttgtca taagtgttct
    13141 catttctata tatattaaca aataaaactt agtgactatt atagtgtata tttatacctt
    13201 ttgcaatata tttattcatt ttttcttaaa aaatgaataa atatcgtaca tttccagaaa
    13261 aatttccaaa aaaagctaga gaatattctc cagcttctaa aatatattcc tcatcataac
    13321 tgattatggt tgataaattt tccgttcttt taaaattttc cagaaataat aagctaaact
    13381 aaaatcaagc aatccatgaa tagccgagcc aacacccacc aagccaagca tcgttatcca
    13441 gtaggtagca gaagtttgtt gccccgaagt aagcaccatc accacaatat attctcctag
    13501 accatgcagg agattaagcc caattcctaa tagtaaaaag cgcttagaat ccatgagagt
    13561 cggaaaacgc ttgaccaaga ccgcacctaa ggtaaggaag aacagatgcg taagcgcccg
    13621 aaatacaata acgattggaa aaccggcgag aaaaaagcct aaactagaac ctaaagcaac
    13681 aaaaatagca gtagctggtg aaataaacat ggctataaac aaaggaatgt gactagcaag
    13741 tgtataggat gctgggccaa taatgatttt aatcggcatc attaaaggta ttaaaatggc
    13801 aaatgcggtt aaaattgcac taattgatag ctgcttaact gaatttttag atgatagtgt
    13861 taattgagac attaggcacc tctctttcta ttggtttgat tataaaacaa aaacattaca
    13921 tgtgtcaaga cacctgtaat gtttattgat tttatagtaa aatacctgca tgttttaaag
    13981 ctgctgaaag ttcattatag gcttgcacag atgggaaaga gactgtatgt gtatgaactc
    14041 cttctgttaa attactcagt aacacacctt ggtattgacc caaggcatcc aaaaaatgat
    14101 ccctatcttc taaacttgca atattcagct tgcctgataa taagccgtaa atgggatgct
    14161 caatttcaac atctagtatt tgagctccac cctctagcaa gattgtcaat tctttttcta
    14221 cctcttctgg accatgttga caagctatct ttcgaatgta agctccttca gtagatggca
    14281 aagaagattg gagtaagtac cctcttggag ttgccaagac agcttctccc tccgcacgaa
    14341 gcaaggctac atctccaaca attacttgcc gactcactcc aaattctttc gccagataat
    14401 tagctgcaag gggatagggg ctttctcgta aaagggatag gattttctta cgtcgttctg
    14461 ttgctttcat acacattcct ttcgtacact tttttccatt atatcaaaat tcctataaat
    14521 caacctttcc tcaaccaaaa aacggaaact gcagaacagt ctccgttatc atctgctagt
    14581 ttagcaatgc ttgaatagcc gcaatgtcca aacgcttcat ggtcatcacc gctgcattga
    14641 ctgccttgac cttttctgga ttagccgtat cgtatgcctc catcaaaata cgcggcacga
    14701 tctgccaaga aatcccaaac tggtccttgg cccaaccgca aatctccgct tctggcacag
    14761 cagacacttt ttcataccag ctctccgctt cttcctgact gtctacatag accatgaatg
    14821 agaaggcttc gttgaaagaa gccacaccac cgtagccgtg gtccatagcg ataatttttt
    14881 ggtctccgat aaaaagctcc gcataattca acttggcagc cgccaccgaa gcctcacctt
    14941 cgccatagta agacaagcga ccaagacgag cttgatgcag cttatccttg tagtaatcca
    15001 acataggctg ggccaaacca acctgttcct gcgagaagag caggcagatg tcaaactgat
    15061 aaggcttgtc caagtccggt gcataagaca gttgccaaga cagaccaaag cggtcttcca
    15121 accagacata gtaagggctg aaaggacatt ctcccaaagg catgagcaca cgaccgcctt
    15181 ctgacaagac ttggtacaaa cgtgtcactt catccttgct agctacattg accatgaggg
    15241 acatggactc attgagctta aagtatggac ccgcactaat agccgccaag gtcatatcgc
    15301 ccagttgaaa ctgaatcaaa tccgaatcac ccgaaggcgt atccgtaatg gtgtaggtcc
    15361 agtcaatctt gccaccgaag agttctacat agaaagccac cgcttctttt gcctctgtat
    15421 cataccagag atgtgggatt atagtttgca ttgatttctc ctttgagtta gtagccccag
    15481 tataacactt gtccgtacaa aaacaaaaca tgttgctttg cacttacaga tgtgctatac
    15541 tgaataacta caaaattgac aggagaatga aatggaaatc tggaacacct acacagccga
    15601 cggtcacttg accgaccaca ccctcacacg cggagaatcc attcccgacg gcctctacca
    15661 cctggtcgtc gagtgcatca tccgccacca cgacggcagt accctcttca tgaaacgcga
    15721 cagcaccaag ccctcctacc ctgactatta tgaagcaaca gcaggaggtt cagctttgtt
    15781 tggggaaata gcagagcagg ccattttgcg cgaagtccgc gaagagacag gcattgagct
    15841 gacagctgac caactccgcc accatacaca ctttgtcgcc catgacgacc agtgcatttt
    15901 ccactgctac tgggctgaaa ccgactggga caaatccaca atccaactcc aatccaacga
    15961 aaccagcaat tatatctggg tcccacaaga aaatttgaaa aatttcctgg aaacagagct
    16021 agtcattcca aggcagaaag actatgtgga gaggttgttt ttaaaaagct aacccccccc
    16081 ttaacagaca aatccccact atcacaggac agcggagatt atttcattac aaactcttca
    16141 acatattgtc aatatgctgg atggatttct cacggccgag caggtaaatg gtgtttggca
    16201 actctggtcc gtgcatttcg cctgaaacgg cgatacggat tggcatgaag aggtttttgc
    16261 ccttgatgcc tgtttctttt tggacagcct tgatttgcgg gaagatgtta tctggttgga
    16321 aatcttcgtc cgtcatggct tccaattttt ccttgagggc attgagaaca gtcggtactg
    16381 tttcgcctgc catcacttct ttttcttccg cagtcaattc tgggaaatct gagaagaaga
    16441 ggtctgtcaa accaacgatt tcatccgcag atgacagttg tggcttgtaa agttccacca
    16501 atttttctgc tttgtctgtc aaacgccccg cttcttccaa gaatggctta gcaaggttga
    16561 aaatagtttc taaatcagca tttttgatgt actcattgct catccagtcc attttcttct
    16621 ggtcaaaggc agctggcgat ttgctgaggc ggttctcgtc aaagagttgg ataaattgct
    16681 cacgggagaa gatttcttcc tcgccacccg ggttccaacc caaaagaccg ataaagttga
    16741 aaacagcttc aggcatgtaa cctttacgac ggtaatcttc tatgaactga agggtgttag
    16801 tatcacgttt agacagtttt ttaccagttt cagaattgat aataagcgtc atgtgaccaa
    16861 attctggtgc ttcccaacca agtgcttcgt agaccatgag ctgcttgggt gtattggcaa
    16921 tgtggtcgtc cccacggata acgtgcgaga ttttcatgag ataatcgtcc accaccacgg
    16981 caaagttgta agttggatag ccgtcacgtt tctggataac ccagtcacca ccgatgttgc
    17041 caccttcgaa ctcgatttcg cccttaacca tgtcattcca cttgtagata cctgtttcgt
    17101 tgacagccaa gcgaaccgtt gggacaatgc cagccgcctc acgctctgcg atgtaggccg
    17161 ctttttcatc ttcagacata ccaaggtatt cgttgatgta gcgaggagtt tcacccgcag
    17221 cttcctgacg ctcacgctcc gccgccaact cttcttcggt cacataagac ttgtaggcct
    17281 tgccttctgc caagagctga tcaacatact tttggtaaat atccaaacgc tcagactgac
    17341 ggtattgctc atgagtttct ggactctcat cccagtcaat ccccaaccaa cgcaggtttt
    17401 caagctgaga acgctcgccg tcttccacat ggcgcttgcg gtctgtgtcc tcgatacgaa
    17461 taaggaaagt accgccgtga tgacgggcaa aaagatagtt aaacaatgct gtacgagcat
    17521 ttccgatatg caaaagtccc gttggacttg gtgcgtaacg cacacgaatt ggtttagtca
    17581 tgtttctctc ctgtaaaatt tcaatcaaga acattatagc atagaacacc cccagataac
    17641 agaaaaatca gtctaaacag actgattttg aggtgttttt tggttacgtg ccagccagta
    17701 gaggatatag aggactaaca aaccagttgc agctaggaaa agtccgctaa tctgattgac
    17761 agtcaagatt aagaccaaac ccgaaaccaa cagtcgagta aaggcttgac ctagcataaa
    17821 ataggttgat accgctccgc caatggtcgc caactgctcc tctggcatag aattgacaaa
    17881 cttagcgtta aacttaggtc cactagctcc tgaactaatc cccattgtag taaggaagaa
    17941 gaaaataatt ggcagttgat gtagcaacat tccgacaaaa acgccaagta gagagaaggt
    18001 tgctgccact tccagcgtca ccatgctggt atttttgaaa agggtaaaga ccaaggtcga
    18061 gcccagaata tgtccgataa agaagatcag aatggtcagg gcaagggttg tttcaacatt
    18121 gaggaagaca aggcctgggt cttcgctaat cagcaggacc agaataggat agagaatcgc
    18181 tccacaggcg ttgataagag gggtggtaat caggaccagg cggaactcgg gtatcttgcg
    18241 cagctccacg atggcttgct tgaaggaagc tagaatgcct ggtctcttgg ttttttgctc
    18301 agtctggctc gactgctctg ctattttcaa aggctgctcc gtcaggagtt tgcgaaagac
    18361 tggcgtcagc agctgcatga tgaggagggc ggctaaaaag gtgccggcat tgagaaaggc
    18421 caggttttgg taggagagga ggccgaccaa gagggcagag agggcctgaa aggcgatatt
    18481 gaggaggctg gtcacggtct ggcgaaaggc cgagtactgc tcccgctctt ctttgggcgc
    18541 tacgcgtaat cccagaggtg tatagaggcc gttttcatag aagccagcga agtctgcaaa
    18601 gacattgaaa gttgcagcca ccacgacaat ccagagggct ggctcaaagc ccatgcagaa
    18661 tccgaggatg aggtatagaa gaacacgaaa gagtagggtc agcttgatag tgctgatttt
    18721 gttgaccgtc ttgtctgcca gatagcctgt aaagagcccc ataaagctag ggaagatttc
    18781 cgagaaggtc acaatcgcca gagccagacg actgttgggc accagcaaaa catagttcat
    18841 caaggccaga taatagacca catcgccaaa attggacagc atatccgccg tcagtaccca
    18901 taggtaggta gcatttttca aaatcttttt catgataatc cctttctttt gaataatttc
    18961 cagaatattc cgaattttta ttttacagag gaacctggat agactttcct ctgcgattat
    19021 tcttttacat agagtaaata tttaatgtag aaaatgccat atcacccgtc accaagagaa
    19081 ccttttcttt tggctcagat gcgtagttgt tagttgttga catggcgcta tccaactcta
    19141 caaccgcttg ccagtcagca ggcagataga gtgtgatatt tgagaaggcc gcatcgatat
    19201 gaaaatctgc cttattaccc agaatcaccg cctgatcaaa atagactgta ctagatccaa
    19261 aggctacatc cacactatca tgaataaaat tctcctcatg gatataacga gtcgaggaac
    19321 caaaagctgt atcgcgtccc ttgtcgccaa tcacccctgt gacaactcca tgagccagtc
    19381 tgcgcttcct tggtttaaaa attagtttca agccaagaca gagtaagaca agcgacagaa
    19441 acatgttcca agtcccaaac tccacccatt catagacaga gttggcagct ataaaagcta
    19501 ggccgaaaca aaagatactg gcaagccaat ccaactttac caagcggtga aaacctgtca
    19561 caaaggttaa tacaaaccag acagtggtcc aaaagggcat tgccaaatgc aaacccaatg
    19621 aatccctcaa caagaccaaa accgccagtc ctataactgc agctccaatt aatagacgtt
    19681 tcatctccct acctcacttc tcgtaacttt tcttttaata aatgataata atgccgcgac
    19741 acgtggactt ccttgtgggt ctgatagaag gaaatccgac tagttcctga aaaagactta
    19801 tccaatgaat acacagctct agcattggca atcgttgatt tagaaacccg acaaaagtag
    19861 atgggcaaat gctccccaag ttcatagagt ttcattttaa cctcgtaggc atcatcgcct
    19921 gtatgagcaa aaatcttggt tccgtccgtt tcaaagaaaa gtatgtcacc caatgaaaga
    19981 aaatactcgc tgctcccctt gtaaaaaaca agcgagggac gactaacttg ttgcaaagct
    20041 tgctgaatct gttcaatctc tggtcccagc tggctggttc ggataacaac ttcaacatga
    20101 tctaagctgt catcaaattc aatcctaact ttcatagctc cctccttttt gatagctcta
    20161 ttataccagt tcgacatcca attacaaggg catttcagta agtggcaaaa ttagacaggt
    20221 aagtggtaga aataaactaa gaaaagtccc ttgcattacg cgagggactt tatttttatt
    20281 atatgatttt gaaaattgca gagtttttct tcttaaaaaa tcgcatccag caacgatgtg
    20341 cttccaccac caccatcacc acctgcaccg acaatatgat tgccaatgac ctgagcaaag
    20401 gtctcaatgt tcaaactctg tacatagatt tctccataac cagtaaaggt attaacgatt
    20461 ccttcacctg taccgataga ttggaggaaa ccattttcca tatggatatt gtagtccaaa
    20521 tcacgactcc aagcaaccac atgagcattg tcaatggtga tacttccacc atccaaagtt
    20581 agcttcttaa tagaaccaaa cgcattcgcc aaaagtgttc cgcgtccctc tgtcgtcatc
    20641 acaaagagtc caccctgacc accaaagaaa gctttcccaa cactctggcg ttccatttta
    20701 tattgagcag aaccatcaag ggccaggaag gcaccatcat ttagtcgata ttgctctgcc
    20761 ccaagttcca aggcaataac ctgacctggt gttgaaggcg caagggcaat ttttccatcg
    20821 tcactattgg aaatggcttg cgtgataaac atagattcgc ctgataccat tgaccgaccg
    20881 atggccccca tcaatttacc aaaacctgaa ccaccacgcg cattgagctt ggtattcaag
    20941 gtcacactcg gcgtatggta gaccatgctc ccctgttgaa tataagctga ttcaccagct
    21001 tccaaggcga tttccaccaa aggaaactgc atattactat cgatagtata tttcatccga
    21061 ttgtctgaca tagttgactc ctatttttta cgaaaggtcc tgttaaccac aggatagcca
    21121 taataaacca agaacaagct agaataaacc aagcatcatt tatctctccg cttgttctgt
    21181 tgtgataata caattttatc tcattcaaaa aaacttgtca aggatttctc ggtcattcag
    21241 ataaaattta caggaaaaac cgaacttcct gagcattcct atataacttc tgtcatacga
    21301 cctgtatcca catcataaac cgcaccagaa atttcaacgt cgtcaggtat gagcggagat
    21361 tgtttcaaga gagcaatatc ctcccgcacg ctttcttcaa tgtctataaa aggaagaaaa
    21421 tcctgaccag ccacatccac tcccaaatct cgcttgagtt ggaccgcaaa ttcttcattg
    21481 gtaaaggtct gggcaccgca gtctgtatgg tggagcacca cgatttcccg tgtgccgagc
    21541 tgctgctggg aaataaccag cgagcgaatc atgtcctccg tcacgcgccc acctgcattc
    21601 cgcagaatgt gagcatcccc caaagccaag cccaaggcct gagccacgtg aagccgtgag
    21661 tccatacagg tcacgatagc caccttggtc tttggacgaa gaggcaaatg gtaatcacca
    21721 tgcaggtcaa cataggcctt gttagccttc ataaagtttt gaaaataaga cataggacct
    21781 ccttgtctga tgagaatttc tgaaattttc agaaataggt tagacctagc ttatcacatt
    21841 cccgactatt tgtcgcaaaa agaggagcga ctagctgtca ctcccgaatg atttcataag
    21901 ttgaatactt tctgcaagac ttcgccaatg gtggtcacgc tgataacggt gatttccttg
    21961 ggcaccttga tacctgtcag ggaatttttt ggggcataga ccttggtaaa gcccaatttg
    22021 gcggcttcgt taatccgttg ttcaatccga ttgacgcggc ggatttcccc tgtcaggcca
    22081 atctcgccta taaagcactc ttgtgggttg gttggcttgt ccttgtaact ggaggcaagg
    22141 gcaactgcga ccgctaggtc aatagctggc tcatccagtt tgacaccgcc tgctgactta
    22201 aggtaggcat cttggttttg gaggagcaga ccggcccgtt tttccaaaac cgccataatc
    22261 aagctggcac ggttgaaatc caagcctgtc gtggtccgct tggcattgcc aaacatggtc
    22321 ggggtcacca gagcctgcac ttcggcaagg atagggcggg tgccctccat ggtcacgaca
    22381 atagccgagc cagtcgctcc gtccagacgc tcttccagaa agacctcact tggattgaga
    22441 acttcgacca agccctgcga ctgcatttca aaaataccga tttcgttggt ggagccaaag
    22501 cgatttttga ccgccctcaa gatacggaag gtgtgttgac gctcgccctc aaaatagaga
    22561 acggtgtcta ccatgtgctc caaggtccgc ggtccagcca gggtgccttc cttggtcatg
    22621 tggccgacga taaaggttgc gatattgttg gtcttggcaa tctgcataag ctcattggtc
    22681 acttcacgga cctgactgac agaaccttgc acgctagaaa tgtcagggct cataatggtc
    22741 tggatagagt cgataatcag gaaatctggc ttgattttct caatctcagt ccgaatgctc
    22801 tgcatattgg tctccgcata gagatagaac tcgctgtcaa tgtcgcccaa acgctccgct
    22861 cgcaacttaa tctgctgggc agactcttcc cccgacacat agaggacggt gccaatagtc
    22921 gaaagttggg tcgatacttg caggagcaag gtcgatttgc caatccctgg atcgcctcca
    22981 atcaggacca gactgcccgg caccacgccg ccaccaagga cacggttaaa ctcttccata
    23041 ttggtcttgg tgcgggccac ttgaatggac gacacttcat tgagcttcat aggacgggtc
    23101 ttctcacctg tcagactgac ccgctcgttc ttgacttcag cgacttccac ttcctcgaca
    23161 aagctagacc aggagccaca gttggggcaa cggcccagat acttgggcga gtggtactcg
    23221 caggattgac agacaaaggt tgtttttttc ttagcgatgg tcatttcctc ctatagagtg
    23281 tattctagga tttcgcagta ggctatagtt tctttgggaa caaactggcg tatcagcata
    23341 ccgtgacaga cgataacgat tcggtcatag gctttgtatt tttcaagggc ctgtaaaaat
    23401 cgctctctca tctccgcagc agtctcatag cgaacaggtg aagactcagg caccgctccg
    23461 ctattttcca aaaataatcg atgagctaag acagcctcgt cttgactggc attttgcccc
    23521 gtcatatctg gtcgccattc atgaaaaaag ggctcgacca gaagaggtag ttgctgggca
    23581 tgagcaatgt aggtcgcagt ttccaaggcc cgtgtgacgc ttgaagcgac gactacttga
    23641 gcttgggaaa agataggatt actagcaatt tcctgagcta aagtccgccc cttttcagtt
    23701 aaaggagcca aatcccgtcc aaaacctgca tacagccgag gattatcatg ctggtcaaac
    23761 atactgtagt cgggttcgga atgccgcaca aagagaatct gcatagtagt ctatttcccc
    23821 gtcgagccaa atccacctgt ccgaacgccg tcggcctggt cgccgtcagc caagaggaag
    23881 ggtgcaaaaa cagcctgcac cattcgctcg ccagcttcca ccacgacagt ttcctctgtg
    23941 atgtttcgca tctgggcaaa gatatgacct tcattggctg gattgccata gtagtcgccg
    24001 tcgataactc caactgagtt aatcaaaacc agccccttct tgcgagggtt ggacgaacgg
    24061 tcgtagagat agaggacctc gtttgcctgc atataggcct tgacacccgt tggaaccagc
    24121 ttaatctcac caggctctaa gctgatcctc tccgcagctt tcaggtcata acctgctgca
    24181 tgagcggtct cacgttttgg caataaattt tcatcagtaa actggctgac cagttcaaaa
    24241 ccacggattt tcatactatt ttttctttta tcctttcaaa cgttgattta ataaggtttc
    24301 tagcaccttg taaaaagttg tttttgtaat ttgtggacca ttttgtacac ctagagatta
    24361 tttatggctt tttcataata tgaaactgcc tctttttctt tatcttttga taggtgccca
    24421 taagtgtcta aggtcattga aatgttagag tgtccaagac gatattggag ttccttgtaa
    24481 ctgataccag cgttcaacag taaactagcg tgagtatggc ggaatgcgtg acaagtaaag
    24541 cgaggaatac ccaaggcctt acaccgacgg tttataattt cttgcagtga aatccgtggg
    24601 aggtattccc taaccgttgt ggcaaagaca acatcaggag cacacgcgcc gacctcaaga
    24661 aacatcaagc gttggcggtt tcgatattgt ttcagtaaga gagctgtttt tttgtctatg
    24721 ctgatgatac gagtacccgc ctttgtcttt gttgtcccga taatatgcag ttccatgcta
    24781 taggttttac tgattgatat tgtgccagct tctaggtcaa tatcagacca ctccagggca
    24841 gacaattcac caatacggca accagtggcc agtaataact taaaaataac atattggtaa
    24901 tacttgctaa agttggtact tgctaattgt tcagcatgat caagaaactg ttttaagtgt
    24961 tcgggttcta tgaacctgac cgcctcacgt ccctttttct gctttttggg taagataaca
    25021 tcacgagctg gatttgtggc cagaacttgc aacagtaccc catgttgtaa aattctatta
    25081 ttgattgaat gaatagcttt aaaatttacc aacttaggag ccagaccatt aacaaactgc
    25141 tgaatggttg ctggtactat cttatctagt ttcattcctc caaatattgg aataaggtgg
    25201 ttatatagtt gccttttggt tactacaaaa gtctgagcct ttactgttaa cttgtaactt
    25261 tctagccaaa ggtcagctaa ttcttgatag gtttttactt ctacttcctt ttttaccgtt
    25321 gaaccatttt tggcaaactc aataggcgcg tgctttgctt tctgtttaac ttctgttttt
    25381 gtcctacctg ttacgctagt ttttactttc ttgccagtaa tggcatctac cccaagatag
    25441 acacttgcac ggtaaacaat gctaccgtct ttttttgtta cttcaataat cttcatgatc
    25501 ataaaccttt ccatcagcag gcaagctgtt attaaaaaga ttttagaatg ttttaggttt
    25561 acatcatgcg taggcttacg agaatagccc tattttcgtt tgttttaagt gggtaggtaa
    25621 attataccag cctaggagaa aatacgatac agacgatttt ggagcgttta acagggttag
    25681 aaaaggtgag attttagagt tcaacacgcg ccgaaggcta aggtttgcta aggtatagct
    25741 aatatataat acattgcacc gtttcaaccg accgcagaca gtttactaaa atatcaaacc
    25801 taacaaaacc taacattttt tcagcaaccg acaaaatccg acttttttat gttgactaat
    25861 gttgactttt ttcactccac acagtccacc agaaagcccc tgagagcgtg gaatagttca
    25921 gtagggtaaa tgtgccagag aagtgttttg acgtggtgag ggggttagtc ttcatcaggt
    25981 tcgatcaatt ttgtcatatc tatatacttt ggttcaattt cacccaatga catttttttc
    26041 aatgctcctg tatttattag ttcagaaaga ctttcaggcg attctatcag ttccttgaaa
    26101 ttgttatgca cccatacgtc gatatttttt ttaatttttt tagtccgaag tggctttgga
    26161 acgatggact gaatcttgtc aatcaaatca aatatttgtt tttttgtttc cttttttgat
    26221 tcatcggagt tataaagttt ataatctaat tccctagcta tttttgtgac ttcggcttct
    26281 gcttgtttat gaacaaattc taatttttta taaagttgtt catccatacc ttcacgtaat
    26341 atttttttta gtttgtaatt cttctggtac atcatcaagc tgtaattgta acgaatttct
    26401 atttcgctag tggttactgt tctgtaaaac atatcttcga ttcctgaaac actgaatgct
    26461 tcatagctcg cccttaatgc tgattcattc gtcccatcca atccattgat aatatctaca
    26521 acaatatctg aaattttttc tatgagttca tcattataat ctgaggtaag caaatgcggg
    26581 aagtctttca cctctttttg tacaagttca aagacacgtt ttttcttatt ttcatcagca
    26641 aaactataga attcaaaccc caacaaaata gactcaatga gattattaac gtttccatat
    26701 agaagttcat ttacagtcat ttgacctaat tcagagattt ttttaagatt ctctttattc
    26761 ggtaaattcc ttcctttttc ccagttatta actgttccct tggaagtttc aaaacgttcc
    26821 ccgaacttct ccatagattc acctaatgaa aggcggattt gtttgattct ttcccctact
    26881 tgaacattgt tattcataac ggaaacacct cctaataact acataataaa ctttttttaa
    26941 aaaaaagtaa agaaaagtat tgatttattt ttaaaaaggt gttatagtct aggtatgcaa
    27001 aagtaaagga ggtggtttaa tgaccagaaa aaacaaaatt cgaggttatc gaaacatgtt
    27061 ggggttgaca caggaacaac ttggaaaaaa gttaggaatg acaaaacaaa gttatcacaa
    27121 taaagaagtt ggtaaaaact cttttagtga tgatgaaaag ttaaagttta aacagctact
    27181 tctgccattg ttccctgata ttacactaga agacatattt ttttaattca aagtatgcaa
    27241 aagtaaagaa agggacaggc gatgaggaga aactacagta actatttaaa tgatttttgt
    27301 aacacttatg gactgagtgg cgcacaggtt gcccgaatgg ttggaatggc tgaggaaact
    27361 attcaagcta ttaggcgagg agagcgaaaa ccccaacagg ctactaagga caaactgaac
    27421 cgctttatct taaattacaa aaaggagaac caaccaatga caccaatcac atcacaggga
    27481 acccaaacgg acattgtggg aattgacagc cgaacagttg cagaatggac aggtaagcaa
    27541 cataaagaat tgatgcgtga tatccgacaa tacacagagt atttgaagag cgcagatttg
    27601 cgctgtactg atttttggca agaaacgact tacaccgcaa gcaatggcaa agaaaatcct
    27661 tgctatatga tcactaaaaa aggttgtgag tttatccagc acaaaatgac aggtcaaaaa
    27721 ggcgcagttt ttacagcaaa gtatatcaat tatttctggg agcagggcca acagttgcaa
    27781 gaagctacac agattttact agacttatca gaccaaccaa cccagccagc tatcgagttg
    27841 aattctgaca tggcatacat caagaaccga ctttcagagc tgcatagtat gaccaccatg
    27901 gcagatatta aagtaggcct agcgaaaact tacagaatcg ccgaactgat ggaagattag
    27961 aaaggagcaa gcaatggaat tagtttacat ggacggatgg aaagagccgt acacattgag
    28021 cagtattgta gcagaatgcg cagaggttaa gcatagacac ttaaaaatgc tgattaacaa
    28081 acacaaggat cgcctggagc gttttggaaa ggtgcatttt aaaatttcac cttcaaaatc
    28141 tgggcagaat gtacgcgact acattttgaa cgagcaacag gcaactctac tgatcacttt
    28201 tctaaggaat accgagcaag ttgcaaactt taaagagaac ctagtccgag cattctttga
    28261 aatgcgtgac gaggtggcag aatttcgcta tcagagggca ctggagaagc caaagcgcaa
    28321 ggcattgcat gaagctattg aaacatggca ggaagctcca aaacacgcgc acagcactgt
    28381 tacaaacctt ttactaaagg gaactactgg aatgaacaaa cgccagctag tggcacaccg
    28441 tggcggattt aatggcattg acagtctaac aagtcaagaa cttatcagat accaggcact
    28501 agaagacatg gctattgcta tgatcaactt aggcatgaca taccaagaga ttaagaatat
    28561 ggtattcaga aaaaacgcac cacaaggcgc gtgaaagcaa caaaaaaagg cttaccgaga
    28621 ccaatcagca aag
//
LOCUS       DASFSW010000008        27528 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.008, whole genome shotgun sequence.
ACCESSION   DASFSW010000008 DASFSW010000000
VERSION     DASFSW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27528
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.008"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(41..1084)
                     /gene="pheS"
                     /locus_tag="U1099_000317"
     CDS             complement(41..1084)
                     /gene="pheS"
                     /locus_tag="U1099_000317"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEM4866667.1"
                     /translation="MSNIEQQLAELSQTTLEKLKEIQHQGEKELQDLRVAVLGKKGSL
                     TDLLKGLKDLSNDMKPIVGKQVNEVRDVLTTAFEETAQKVAAAKIQQQLASETIDVTL
                     PGRQVKVGKRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDM
                     QDTFYITEEILMRTHTSPVQARTMDQHDFSKGALKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGENVSMGDLKGTLEMIIKKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     DGCNVCKKTGWIEILGAGMVHPQVLEMSGIDSTKYSGFAFGLGQERIAMLRYGINDIR
                     GFYQGDVRFSEQF"
     gene            complement(1539..3242)
                     /locus_tag="U1099_000318"
     CDS             complement(1539..3242)
                     /locus_tag="U1099_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEM4866668.1"
                     /translation="MPRLVDLRKKPYCLNDEQITWVEDTLAHLTDEEKIGQLFVNLFF
                     FGGDAFSGNNLSNQELMNRYHIGGARYMNGSPEQVQGLINELQSYSKIPLLVAANCDS
                     GGDGAVKGGTYISSGAQSEASRDPRIPYLAGLVSAREETALGVNVNFDPCVDIVQNWR
                     NTIVNTRAYGTNADDVIAYTSAYLKGLTAERDVIQCIKHFPGDGTEERDQHLVLGVNE
                     LSPEEWDKSFGRVYRHHIEAGVEMIMAGHIALPYYQQRLNPTLKDEDILPATLAPELI
                     QDLLKEKLEFNGLVITDASHMLGMTAAMRREDYVPAAIAAGCDMFLFFNNLEEDFEFM
                     LNGYRKGVITEERLHDALRRILGLKAKLNLHIKQAEGTLLKSADELAIIGCEEHLAWQ
                     REAADKAITLLKDTQKNLPISPETHRRIRLYYLEGEKEGIMAASTEVVDNLKAALEAR
                     GYEVTVNDGTTRIKGKTLQYREEVDLALTVANVIGYGAQNNYRIQWKTAMSNEVPTIF
                     VSLNYTTHLHDVTMVKTAINAYHHNINTIESLLDKLEGKDDFYGVPNENVWANKWQAK
                     L"
     gene            complement(3253..5043)
                     /locus_tag="U1099_000319"
     CDS             complement(3253..5043)
                     /locus_tag="U1099_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEM4866669.1"
                     /translation="MTHLVDLRKKPYSLDEEAISWIEETIAQMTLDEKIGHLFVNMGS
                     QRTEEYLTGVLNDYKIAAVRYNPGPAADIWEQNYILQTKSKIPLLIAANTESGGNGAV
                     TDGTKIGDEIKIAATADPRYAYEMGKVAGLEAAAVGCNASFAPIMDLSRNWRNPIIAN
                     RTWGANVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWM
                     ETFGRIYGEMADAGLPGIMAGHIHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMIGMTASMSRRELLPTAIEAGCDLFLFFNDPEEDLQWMKEGLENG
                     LLSEERLHDALRRTLGLKAKLGLHLFEGRRQEIMMPKEEALALIGRDEAKQLAKEVAD
                     KAITLVKAKQEGIFPVNPERYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEAR
                     GHQVSIWENTEARIAKLPEEERPAAIANVYASKRPIAEITDNYDLIINLVDVNSGGTT
                     QRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPFALADMPQVATYINAYDGLPVTLEALV
                     AKLAGESDFTGVSPVDAYCGLVDTQIWRGK"
     gene            complement(5093..5893)
                     /locus_tag="U1099_000320"
     CDS             complement(5093..5893)
                     /locus_tag="U1099_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4866670.1"
                     /translation="MMKTDYVFCVDSDGCAMDTMTYKHKLFFGPLAADVFDVKDREPF
                     LAEWNRVNLYSRERGINRFVGLVKGLDFAGVTGIDNLKNWVATTDSLSNASLERLIEE
                     TPSKDLELALEWSTQVNQAIKHYSGPVLAFIGVHKGLEKLSQLGKVYVVSSANKEAVE
                     EEWTDQGLLDFVTELYCQDRGKKEDVIKILIEEGYCPDKIMMIGDSPGDLKAAELNGV
                     HFYPILVGQEMQSWADLTETIADEFAHQAFTGEKETELIQAFWNNLDD"
     gene            complement(5893..6732)
                     /locus_tag="U1099_000321"
     CDS             complement(5893..6732)
                     /locus_tag="U1099_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM4866671.1"
                     /translation="MTRVIEFKDKVVVITGAGGVLCGYLAKEFAKAGAKVALLDLNEA
                     AAQVFVDEIAAAGGVAKAYKSNVLSKENLEGVRQQVLADFGPVDILINGAGGNNPKAT
                     TDNEFHEIGLPADTKTFFDLDEAGINFVFNLNYLGTLLPTQVFAQDMIGRSGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRGLLF
                     DESGQPTARANKILTNTPMGRFGEAEELVGGVFFLADENLASFVNGVVLPIDGGFSAY
                     SGV"
     gene            complement(6734..7834)
                     /locus_tag="U1099_000322"
     CDS             complement(6734..7834)
                     /locus_tag="U1099_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEM4866672.1"
                     /translation="MKMSFRWYGKKDPVTLEEIKAIPGMQGIVTAVYDVPVGQDWPLE
                     NILELKKMVEEAGLEITVIESIPVHEDIKQGKPNRDELIENYKTSIRNVGAAGIPVVC
                     YNFMPVFDWTRSDLHHPLPDGSTSLAFLKSDLAGVDPVADDLNLPGWDSSYSKEEMKA
                     IIENYRQNISEEDLWANLEYFIKAILPTAEEAGVKMAIHPDDPPYGIFGLPRIITGQE
                     AVERFLNLYDSEHNGITMCVGSYASDPKNDVLAMTEYALKRNRINFMHTRNVTAGAWG
                     FQETAHLSQAGDIDMNAVVKLLVDYDWTGALRPDHGRRIWGDQTKTPGYGLYDRALGA
                     TYFNGLYEANMRAAGKTPDFGITVKTVGNKES"
     gene            complement(7849..9249)
                     /gene="uxaC"
                     /locus_tag="U1099_000323"
     CDS             complement(7849..9249)
                     /gene="uxaC"
                     /locus_tag="U1099_000323"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEM4866673.1"
                     /translation="MSFNDKNFMLKNEPAKELYGKIAELPIYDFHCHLDPKEIFEDKV
                     YEDIVDLWLGGDHYKWRLMRANGISEEEITGSASKLDKFKAWARTLERAFGNPLYHWS
                     HLELRQVFGVEELLTEENAERLYHQLNAYLQEHRVSPRKLIADARVTFIGTTDHPLDS
                     LEWHKRLAEDSTIDTVVAPTFRPDEAFIEHRNFNLFMKRLEEVTGVAVRDFASFVEAL
                     GQRVSYFAQHGCRASDISFTAISYEEATLEELDEILLARLAGKEAGQSSINKWQTAIF
                     RELCRLYKKHGFVTQVHFGALRNNHTGLFEKLGADVGVDSIGDQTCLTVNLNRLLDNL
                     VKENALPKMIWYNLNPSYNIALANTLANFQSNEEGLRSQLQFGAGWWFNDTKLGMIDQ
                     MNAYAEQGMLANFVGMLTDSRSFLSYQRHDYFRRILATYVGQWIVDEEVPEDYNRLGQ
                     FVEAISYYNAKEFFEQ"
     gene            complement(9251..9871)
                     /gene="eda"
                     /locus_tag="U1099_000324"
     CDS             complement(9251..9871)
                     /gene="eda"
                     /locus_tag="U1099_000324"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEM4866674.1"
                     /translation="MLKQLKENYFFAVIRGKDEEDAKEIARHAILGGIRNIEITFSTP
                     NAATVIKELQEEFSYDSSVVIGAGTVMNLKLAQAAIDAGASFLVSPHFDKEIQDLAQE
                     AEVFYFPGCATATEIVTASQAGCPIIKLFPGGVLGPGFIKDIHGPVPQVDLMPSGGVS
                     VENVADWKKAGACAVGVGSALASRVQAEGYESVTTIARSFVAALEG"
     gene            complement(9994..10710)
                     /locus_tag="U1099_000325"
     CDS             complement(9994..10710)
                     /locus_tag="U1099_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEM4866675.1"
                     /translation="MAKPLVEKASERLLELILERGYEVGDKLPNEYELAQDLEVGRST
                     VREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLVRDRVKLTTDLFEVRYLLE
                     PRIAERVAQFASDEDIARLEEIVLAIEEAVAAGDTKHLELDVKFHCMLAEMSGNLAMT
                     SLMPVINQSIHLINENYSNRQMKSDSLQAHRNILMAIKQRHPVAAYDSMLAHIISVRQ
                     VVLSEWFDKDMTLHGLNDHK"
     gene            complement(10756..12555)
                     /gene="uidA"
                     /locus_tag="U1099_000326"
     CDS             complement(10756..12555)
                     /gene="uidA"
                     /locus_tag="U1099_000326"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEM4866676.1"
                     /translation="MLYPIQTKTRSVYSLNGIWKFKQGNNDVHTLLDTDEVMVVPSSF
                     NDLVVDKDKRRFVGDNWYELMVALPVVSADEELVVRFGSVTHQAKVYADGQLIGEHKG
                     GFTPFECLIPSNLYDADQFRLTVCANNELNYTTLPVGNYSEEVDENGQIVKTVKENFD
                     FFNYAGIQRTVHLYKRPQNRIEDIVIRTELNQDLTQAVVNVDVKTVGQYDSIRMSILD
                     QEGQEVGLLENGQMVIEHPRLWEVLDAYLYTAKVELFDGENLLDTYSEQFGIRSVAVE
                     NGQFFINGKPFYFKGFGKHEDTFINGRGFNEAANLMDLNLLKDIGANSFRTSHYPYSE
                     EMMRLADRLGIVVLDEVPAVGLFQLFNAALNLTGDSEEVKSTWEVMQTKDAHELVIDE
                     LIARDKNHPSVVMWVVANEPAGHEKGARAYFEPLIQRMRDRDPSKRPVTLVNIMTATP
                     DKDEVMDLVDVVCLNRYYGWYVAHGDLKAAEKGLRQELETWQKLYPDKPILITEYGAD
                     TLQGLHSMWDIPYTEEFQVDYYDMNHRVFDSIPNLVGEQVWNFADFETTVGIIRIQGN
                     HKGLFSRNRQPKQIVREIKKRWTAIPNYHYKRK"
     gene            complement(12571..13599)
                     /locus_tag="U1099_000327"
     CDS             complement(12571..13599)
                     /locus_tag="U1099_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEM4866677.1"
                     /translation="MKKILAFGEVLLRLSPPQYQTLTQATSLDCQFGGSELNVLASLA
                     QLGHSVELVTALPKNEVGKMAEHFLFARQIGQKYVIRNEGRLGLYYYQKGFSLRPSQV
                     TYDRDYSAFSLSKEEDYDLEGMFDGVDWFHVSGITVALNPDIYRLAFRLMEMAKGQGV
                     KVSFDLNYRESLWSSFEEAREKLSPFVGLADICFGLEPIQLFNESGKDLKDELGLKRP
                     YENKEVLLAVVKGLAETYSLQGLAFTQREMTYNNEYLLKAYLYQDGILYETEKESIQV
                     LDRVGTGDAFTAGIILGYLQEKSPREILDLGMASFQFKHTIEGDINVISQSDIDLLLK
                     KGSREIKR"
     gene            complement(13655..15208)
                     /locus_tag="U1099_000328"
     CDS             complement(13655..15208)
                     /locus_tag="U1099_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM4866678.1"
                     /translation="MEKHVEQRVEDVTYNKAKLWQIILFAMNNSSTNIYLVAFTFVTY
                     LSTGVLGLAALFVSQLMGYIRIFDGFIDPTIGVIIDKTDTKFGKYRPILVLGNIITAA
                     SLFMMLGLSGVNESLRLPLFILVLIIHKIGYSLQQTITKAGQVALTNDPKQRPIFNIV
                     DGIATTVLMTGSQVVVSGTLVPKHGGFTPSFFQELFLGVVVISAVLGILAIIGIWEKD
                     NKKYFGLGEKTQETKLKDYWKVIKGNKPLQVLSISAAFVKFVAQLFGDSVIGVMLYGI
                     IFGDYALSGKMSMLLILPGILVTVGAAQLARRKGLRFAYITALQLGIAGLVILAAMLF
                     SAKPGMLDLTNWNLYTIGFFVVYIVARYASSAPSGLVLTMGADITDYETSVSGRYLSG
                     MIGTIFSLTDSIASSFAPMVVGFVLAGIGFGKEYPTVETAFSTDLRMALIVLLTVIPA
                     AVLSVSLFLMKFYKLDREEMARIQEKIHVMKAAKDEERVRAIAKNVPLSDMDYVDVTQ
                     YKVDQYDDK"
     gene            complement(15395..16234)
                     /locus_tag="U1099_000329"
     CDS             complement(15395..16234)
                     /locus_tag="U1099_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEM4866679.1"
                     /translation="MAKKNVRKQAMSLLSLLATIVVVAGGYLLSDNKSSNVADYSYYV
                     NQSKASEGTPSQELASSVLTEDVKKQLGNSIEWNGAGAFIINGNQTDLDASVASVPYG
                     DNKTKTVQGETVPTVANALLAKSTRQYKDREETGNGRTSWTPAGWHQIHDLTGEYDHA
                     VDRGHLLGYALIGGLSGFDASTSNPKNIAVQTAWSNQANDQTSTGQNYFESQIRRALD
                     KNKRIRYRVTLVYAESEDLVPVGSHLEAKAADGSLEFNVFVPNVQSGLSINYHSGEIA
                     VNQ"
     gene            complement(16264..16455)
                     /locus_tag="U1099_000330"
     CDS             complement(16264..16455)
                     /locus_tag="U1099_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM4866680.1"
                     /translation="MDKDNLSYVGKNLILVAVVLLIAILVFVLGLMVGYGVIGDGDNV
                     FAVLSPAKWQELIGKFTGK"
     gene            complement(16448..17737)
                     /gene="murA"
                     /locus_tag="U1099_000331"
     CDS             complement(16448..17737)
                     /gene="murA"
                     /locus_tag="U1099_000331"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM4866681.1"
                     /translation="MDTIIVTGSKNGLKGQVEIEGAKNAVLPLLAATVLASQGQTKLT
                     QVPILSDVYTMNNVVRGLGVRVKFDEETKTIDIDATGKLGSETPYKYVSQMRASIVIL
                     GPILARNGYAKVSMPGGCTIGSRPIDLHLKGLQAMGAKIKQRAGYIEASAENLQGAHI
                     YLDFPSVGVGATQNLMMAATLAQGTTVLENAAREPEIVDLAIFLNKMGAKVKGAGTET
                     ITITGVEELTGAEHQVVQDRIEAGTFMVAAAMTGGDVLVKDAVWEHNRPLISKMLEMG
                     VEVTEEAEGIRVRSQVDKLKPVTVKTLPHPGFPTDMQAQFTALMAVAKGESTMIETVF
                     ENRFQHLEEMRRMDLHSEILRDTAIITGGLALQGAQVMSTDLRASAALILAGLVAEGE
                     TIVGKLTHLDRGYYRFHEKLAALGATIERVSGEDENG"
     gene            complement(17762..17986)
                     /locus_tag="U1099_000332"
     CDS             complement(17762..17986)
                     /locus_tag="U1099_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEM4866682.1"
                     /translation="MVISILQFFSHILFIYLAHHLLVTTVDWSRWLKVTGDNQRKINL
                     LILFLAIALGYLVSTFFLELLMVGRSFANM"
     gene            complement(18046..18462)
                     /locus_tag="U1099_000333"
     CDS             complement(18046..18462)
                     /locus_tag="U1099_000333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEM4866683.1"
                     /translation="MGQMTVQIVTPDGIKYDHHAAFVLVKTVDGEMGVYSGHEELIAV
                     LEIGEMKVRRVDDENHVDWIAVNGGIIEVNKDIITVISDSAERERDIDISRAERAKLR
                     AERELEEAQTARNIDMEMRATVALQRAINRIRVGKH"
     gene            complement(18472..19878)
                     /gene="atpD"
                     /locus_tag="U1099_000334"
     CDS             complement(18472..19878)
                     /gene="atpD"
                     /locus_tag="U1099_000334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEM4866684.1"
                     /translation="MSSGKITQVVGPVVDVAFAAEDKLPEINNALVVYKNDDSKQKVV
                     LEVALELGDGVVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EESFPADFEREPIHKKAPAFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     LGIYPAVDPLASSSRALAPQIVGEEHYAVAMEVKRVLQRYQELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGMPGSYVPVAETVKGFKEILDGKHDHLPEDAF
                     RNVGSIEDVVAKAAKMKF"
     gene            complement(19898..20776)
                     /locus_tag="U1099_000335"
     CDS             complement(19898..20776)
                     /locus_tag="U1099_000335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEM4866685.1"
                     /translation="MAGSLNEIKSKIASTKKTSQITGAMQMVSASKLAKSEQLAQSFQ
                     IYASKVRQITTDLLRGELMASDTSNPMLIRRPVQKSGYIVITSDSGLKGSYNSSILKA
                     VMGMIEQDHDSKDEYEIIAIGSMGADFFRARGINPVFELRGLADNPSFEEVQKIISKS
                     VEMYKNELFDELYVCYNHHVNSLTSQVRVQQMLPVEDLDHNEADGYTATFELEPNREV
                     ILEQLLTQYAESTIYGAILDAKTAENAAGMTAMQTATDNAKNVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(20788..22308)
                     /locus_tag="U1099_000336"
     CDS             complement(20788..22308)
                     /locus_tag="U1099_000336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEM4866686.1"
                     /translation="MESGVLLINAQEISALLKQQIEGFQPDFDYTETGVVTYIGDGIA
                     RAQGLDNAMSGELLVFENGTIGMAQNLETNDVGIIILGQFTDIREGSVVRRTGKIMEV
                     PVGSALIGRVINPLGQPVDGLGEIRTSKTRPIEYPAPGVMQRKSVNEPLQTGLKAIDA
                     LVPIGRGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLR
                     QYGALDYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYEGKHVLIVYDDLSKQAVAYR
                     ELSLLLRRPPGREAYPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAY
                     IATNVISITDGQIFLKDDLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLA
                     SYRELEAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVLILYALTNGFL
                     DSVPIDDILAFEEELYSYFDLHYDGLLDVIRTTKDLPDTDELNAAIQAFKDQSVFK"
     gene            complement(22312..22845)
                     /locus_tag="U1099_000337"
     CDS             complement(22312..22845)
                     /locus_tag="U1099_000337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEM4866687.1"
                     /translation="MNARENAIVQKYALSFVEKVSDHADIWDMYDQISDLISIIHDSK
                     LNRILLSATVSREEKADFVRTVRQSSFWQINDLIEDVIRDGHADLLLETLERVQLQIS
                     KFKNEFEARVVSVYPLTEAQKERLRHLVEQRFSLRVRNITEELDQSLLGGFIVTVNHK
                     VIDASVRTQLKDVRKKL"
     gene            complement(22846..23352)
                     /gene="atpF"
                     /locus_tag="U1099_000338"
     CDS             complement(22846..23352)
                     /gene="atpF"
                     /locus_tag="U1099_000338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEM4866688.1"
                     /translation="MATTITMQSSTILGNFILVTASFAVLIILIRVFAWDKITGIFEE
                     RANKIANDIDAAEEKLTAAANLVQQREDELVQGRIESQKIIQDAVERAKLEKKRILEQ
                     ADVEIQGLKQKAQLEIEAEKREAQENLRVQVAELAVDLASKIILEDLDQQAHSNLIDR
                     YLDKLGDK"
     gene            complement(23370..24086)
                     /gene="atpB"
                     /locus_tag="U1099_000339"
     CDS             complement(23370..24086)
                     /gene="atpB"
                     /locus_tag="U1099_000339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEM4866689.1"
                     /translation="MEEHLSPTLTLGPVTFDLTMVLVSVITISIIFLLVFWASRRMEL
                     KPKGKQNVLEYVYELTINFTKGNLGDAEAKRYSLFFFTVFTFLLVANNLGLMTKLETA
                     EGHNLWTSPTANMAYDFGLATIATVFCHVEGIRRRGFKAYLKSFVTPWAMAPMNILEE
                     VTNLVSLALRLYGNIYAGEVLVSLLLQLSQQSALAYPIAFALNVVWTAFSVFISCLQG
                     YVFIMLVSMYLNKKISSESE"
     gene            complement(24123..24323)
                     /locus_tag="U1099_000340"
     CDS             complement(24123..24323)
                     /locus_tag="U1099_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEM4866690.1"
                     /translation="MSLGALALGLACLGVSIGEGLLVASYLSSTARQPEMQSKLMAGV
                     FLGVAFIEGTFFVTLAMTFVLK"
     gene            complement(24460..25503)
                     /locus_tag="U1099_000341"
     CDS             complement(24460..25503)
                     /locus_tag="U1099_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEM4866691.1"
                     /translation="MEERKRARLIYNPTSGQEIMKKNVAEVLEILEGYGYETSAFQTT
                     AEKDSAKNEATRAALADFDLIIAAGGDGTINEVVNGIAPLEKRPQMAIIPTGTTNDYA
                     RALKVPRGNPVEAAKVIGKQQTILMDIGLAKNQKNGFHQEHYFINIAAAGTLTELTYS
                     VPSQLKTMFGYLAYVVKGAELLPQVQFTPVRVEHDEGVFEGSVSMIFVALTNSIGGFE
                     QIVPDAKLDDGNFTLLMVKTGNLFEILHLIRQVLDGGKHIESDLVEYIKTKSLSIENL
                     NPDNRLLLNLDGEFGGEAPVRLHNLSNHIEFFADTDLVSDHAITLDTDQMNREDMAKR
                     FIEETDHIDEAIK"
     gene            complement(25514..27472)
                     /gene="ligA"
                     /locus_tag="U1099_000342"
     CDS             complement(25514..27472)
                     /gene="ligA"
                     /locus_tag="U1099_000342"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEM4866692.1"
                     /translation="MKTRISELVSVLNQYAKEYYQLDQPSVSDAEYDTLYRELVELET
                     AHPELILPDSPTHRVGGKMLDGFEKYSHVYPLFSLQDAFSREELEAFDQRVRKEFPQA
                     TYICELKIDGLSISLTYEAGNLVVGATRGDGSVGENITENLKRVADVPLTLPEAVDIT
                     VRGECYMPKASFDRVNKQRQEAGEAEFANPRNAAAGTLRQLDTGVVAQRGLATFLYQE
                     ASPSEATSQSQVLEKLDALGFVTNHEYCLAESIDDTWDFIEKIAERRDDLPYEIDGVV
                     IKVNDLAIQEELGFTVKAPRWAVAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLSPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLKVVDKYRKEQEIMPIP
                     SHCPSCQSDLQHYEDEVALRCINPICPSQLMSKLEHFASRDAMNIAGLGSSIVEKLFG
                     AGLVHDVADIYKLTVDDLLTLEGFKEKSADKLYQAIQTSKSNSAECLLFGLGIRHVGS
                     KASKILVEKFGDLETLAFADQEAIASLEGLGQVIAKSLTTFFASEGAQQLLAELKEAK
                     VNLTYLGQVVDENAALSGMTVVLTGKLERMKRNEAKAKLEALGANVAGSVSKKTNLVV
                     AGTDAGSKLTKAQELGIEIKDEAWLESL"
BASE COUNT         7933 a         6610 c         5006 g         7979 t
ORIGIN      
        1 ctgtttctag atactttcag tatcagaaac agctctcaca tcaaaactgc tccgagaatc
       61 gtacatcgcc ttgatagaat ccacgaatat cgttgatacc gtagcgaagc atggcaatac
      121 gttcctgacc aagaccgaag gcaaagccag agtacttggt tgagtcaatg ccactcattt
      181 ccaatacttg cgggtggacc atgccggcac cgaggatttc aatccagcct gttttcttac
      241 atacattaca gccgtcacca ccgcacttaa agcaggaaac atccacctca acagaaggct
      301 cagtgaatgg gaagtatgat ggacgcaggc gaatctgacg ctcttcacca aacattttct
      361 tgataatcat ttcaagcgta cccttgagat cgcccatgga tacattttca ccgacaacca
      421 aaccttcgat ctggtggaac tggtggctgt gagtcgcatc atccgtatca cgacggaaaa
      481 cacgtcctgg cgaaatcatc ttcaaggcac ctttagaaaa atcatgttgg tccatggtac
      541 gtgcctgaac aggcgacgta tgggtccgca taagaatttc ttctgtgatg tagaaggtgt
      601 cctgcatatc acgcgctggg tggtcttttg gcaaattcat gcgctcaaag ttgtagtagt
      661 ctttttccac ttcaaatccg tcaacgattt ggaagcccat gcccaagaaa atatcttcga
      721 tttcctcaga agtttgggtc aagacatggc gttttccgac cttaacctga cgacctggca
      781 aggtcacatc aatggtttcg gaagctagtt gttgctgaat ttttgctgct gcaacctttt
      841 gagcagtttc ttcaaaggca gttgtcagca catcacgcac ttcattaacc tgtttaccaa
      901 cgattggttt catgtcgtta gacaggtctt tcaagccttt caataagtcc gtcaaagacc
      961 cttttttacc caaaaccgca acacgcaggt cttgtagttc tttttctcct tggtgctgga
     1021 tttcttttaa cttttctagt gtcgtttggc ttaattcagc caactgttgt tcaatgtttg
     1081 acataattcc tccaataaaa aacgcatgac accactcgta tcggagcgtt gtcacgcggt
     1141 accatccgtt ttcatctgca aggcagacct tagttctatg aacctatatt cacaagtgaa
     1201 gtgcttgcac ataagagatg gttttccgct aatcaaggct gttttttgag ggaatactga
     1261 ctgtattttg aaaaaaacta acgatgagta gctgaaaaac tagccttaga tggaagtgtt
     1321 gaactgtgaa tgcaggttct aagtccttgt gtgagtgaat tcgaccaact ttcactgatt
     1381 gagcttacag tcacggctca actccctggg cagtttccat aggtgtacta gtctcacaga
     1441 ctcctattca attaagaata ttctatcaaa aagaagaaag tttggcaaga aatcttccta
     1501 gaaaactccc tctatctagg atagaaagga gtttagtttt atagttttgc ctgccattta
     1561 ttggcccaaa cattctcatt tggaacacca tagaagtcat cttttccttc taatttatca
     1621 agtaaggatt cgatagtatt tatattgtga tgataggcat tgatagcagt tttgaccatg
     1681 gtaacatcgt gaaggtgagt tgtatagttt agtgaaacga aaatggtcgg aacttcattg
     1741 gacatagccg ttttccactg gatgcggtaa ttattctgtg ctccatatcc aatgacattg
     1801 gcaacagtca aggctaaatc aacttcttct cggtattgca aggtttttcc tttaatccga
     1861 gtcgtaccat cattgacagt cacttcataa ccacgcgctt ccaaggcagc tttaaggtta
     1921 tcaaccactt ctgtacttgc cgccataatc ccttcttttt ccccttctag gtaatacaaa
     1981 cggattctgc gatgggtttc tggactgatt ggaagatttt tttgtgtatc tttcaacagc
     2041 gtaattgcct tgtctgctgc ctcacgttgc caagccaagt gttcttcaca gccgataata
     2101 gccaattcat cagctgattt tagtaaggtt ccttccgctt gcttgatatg cagattgagt
     2161 ttggctttca gacctaaaat gcgacggaga gcatcatgaa ggcgttcttc agtaatcact
     2221 cccttgcggt agccattgag cataaattca aaatcttctt ctaagttatt gaagaataag
     2281 aacatatcgc aacccgcagc aatggctgca ggtacgtagt cttcacgacg catggctgct
     2341 gtcatcccca acatgtggct ggcatctgtt atgaccaagc cattgaattc cagtttttct
     2401 ttcaataaat cttgaatcaa ttcaggagcc agtgttgcag gcaaaatgtc ttcatccttc
     2461 aaagttggat tgagtcgttg ttggtaatat ggcaaggcaa tatgacccgc cataatcatc
     2521 tcaacaccag cttcaatatg atgacgatag acacgaccaa aactcttgtc ccattcttct
     2581 ggagagagtt cattgacacc gaggactagg tgctgatcac gttcttctgt accatctcct
     2641 gggaaatgct tgatacattg aataacatct ctttcagcag tcaatccttt taaataggca
     2701 ctggtatagg caataacatc atccgcattt gtaccatagg ctctggtatt gacaatcgta
     2761 ttgcgccagt tctgaacaat gtccacgcaa ggatcaaagt tgacatttac acctaatgca
     2821 gtctcctcgc gcgcagatac gagacctgct aggtagggaa tacgaggatc ccgactggct
     2881 tctgattgag cacctgagga aatataggtt cctcctttca cagccccatc tcctcctgag
     2941 tcacagttcg ctgcaaccaa gagaggtatt tttgaatagc tttgcaactc gttaatcagt
     3001 ccttgtacct gttctggact gccattcata taacgagcac cacctatgtg atatcggttc
     3061 atcaattcct gattactcag attatttcca ctaaaggcat cccctccgaa gaagaataga
     3121 ttaacaaaca attgaccgat tttttcttca tcagtcagat gagccaaggt atcttctacc
     3181 caggtaatct gctcatcatt caaacaatag ggtttcttcc gcaaatcaac caatctaggc
     3241 attctttcct ccttattttc cgcgccaaat ctgtgtatct accaagccac aataggcatc
     3301 tacaggcgat actcctgtaa agtctgactc tcctgctagt ttagcaacca aggcttctaa
     3361 tgtgacaggc aagccatcat aggcattgat ataggtagca acctgtggca tatctgcaag
     3421 tgcaaatggg tgctgaacag agacaacaat ggttggaatt tcgtgaacat agaaaggctg
     3481 gtctggcgtt cccttggcag ctggccaaat aattcgctga gttgttcctc ctgagttgac
     3541 atcaactaaa ttgataatca aatcatagtt atctgtgatt tcggcaattg gacgcttgga
     3601 agcatagaca tttgcaatcg ctgctggtct ttcttcttct ggtaatttgg caatccgtgc
     3661 ctccgtattt tcccagatag atacttggtg acctcgagct tctaatagtt ctttcagcgt
     3721 atcggctgct ctcttcttgc cagcattgat catggcgcca aaaccaccct tatagccatc
     3781 cacttcgacc aataaaatac gtttgtagcg ttctggattt acggggaaaa ttccttcttg
     3841 tttggctttt accaaggtaa tcgccttgtc agctacttct tttgcaagct gtttcgcctc
     3901 atctctacca atcaaagcca gtgcttcttc ttttggcatc atgatttcct gacgacgacc
     3961 ttcaaacaag tgaagaccga gtttagcttt caagcccaag gtacgacgaa gggcatcatg
     4021 tagacgttcc tcagaaagca gaccattttc caatccttct ttcatccatt gcaaatcttc
     4081 ttctggatca ttgaagaaaa ggaaaagatc acaccctgcc tcaatagctg taggcaatag
     4141 ctcacgacgc gacatagagg ctgtcatacc gatcatgtga ctggcatccg tgacaatggc
     4201 accattgtaa cctaattctc cacgaagcaa ttcatccaag agggttttat tcagcgaagc
     4261 tggcaacatg tcatccaatt cacgttctgg atgcatttct ttttcgacat ttggcaaatg
     4321 aatatgcccc gccataatac ctggaagacc tgcgtcagcc atctcaccat aaatccgtcc
     4381 aaatgtttcc atccattcag atttggtcat gggattcgaa gcaaaagaga gatgatggtc
     4441 acgttcatca ataccatcgc ctgggaaatg cttagcaaac ggcacaatgc catgttccat
     4501 aatgcctcgc atgtattctt tggacaattc aatgacttgg tctacattag ctccccaagt
     4561 tcggtttgca atgattggat tgcgccaatt acggctgagg tccataatcg gtgcaaagga
     4621 ggcattgcag cctactgcag ctgcttcaag tcctgcaact ttccccattt cataagcata
     4681 gcggggatca gctgtcgcag caatcttgat ttcatcccca attttcgttc cgtccgtaac
     4741 agcgccattc ccaccagatt cggtattggc tgcaatcaaa aggggaatct tagacttggt
     4801 ctgcaaaata tagttttgct cccaaatatc tgcagcaggt ccaggattgt aacgaacagc
     4861 agcaatctta taatcattta atactccagt cagatattct tctgtccgct ggctccccat
     4921 gttgacaaag aggtgaccga ttttttcatc caaggtcatc tgagcaatgg tttcttcaat
     4981 ccaagaaata gcctcttcat caaggctgta aggtttcttc cgtaaatcta ctaaatgtgt
     5041 catgtttgcc tccaaaaaag ctagagggca tcgcgcctct agcccttctc tactagtcgt
     5101 ctaaattatt ccaaaatgcc tgtatcaatt ctgtttcttt ctcacctgtg aatgcttgat
     5161 gagcaaattc atctgcaatc gtttctgtca aatccgccca agattgcatc tcttgtccta
     5221 ctaaaatagg atagaaatgg acaccgttca attccgccgc cttcaaatca ccaggcgagt
     5281 caccaatcat cataatctta tcaggacaat acccttcctc tattaatatt ttaatgacat
     5341 cttctttctt gccacggtct tgacagtaca attctgtaac aaagtccagc aaaccttggt
     5401 ctgtccactc ttcttctact gcttccttat tagcagaaga aaccacgtag acttttccta
     5461 actggctcaa tttttccaag cccttgtgaa cgccgatgaa ggctaaaaca ggaccgctat
     5521 aatgcttaat ggcttgattg acttgagtgg accattctaa agctaattcc aagtcctttg
     5581 atggcgtctc ctctatcaat ctttccagag aagcatttga caaagaatct gtggtcgcga
     5641 cccaattttt caagttatct ataccagtca cacctgcaaa gtctaggcct ttaactaagc
     5701 ctacaaaacg gttaatgcca cgttctcgag agtacagatt gactcgattc cattcagcta
     5761 aaaacggttc tctatctttg acatcaaaaa catctgcagc caaggggccg aaaaagagtt
     5821 tatgtttgta ggtcatggtg tccatggcgc agccatcaga gtccacacaa aatacgtaat
     5881 ccgttttcat catcaaactc ctgaataggc tgagaagcca ccgtcaatcg gtaaaacaac
     5941 accgttgaca aaacttgcca aattctcatc tgccaagaag aagactccgc cgactagctc
     6001 ttccgcttca ccaaaccgtc ccattggtgt attggtcaaa atcttgttgg cacgtgcggt
     6061 cggttgacct gactcgtcaa atagcaagcc acggttttgg ttggtaacca agaatccagg
     6121 cgcaatcgca ttacaacgga tgccaacttt tgaaaaatgg actgccaacc attgggtaaa
     6181 attactaata gcagccttgg cacctgaata ggctggaatt tttgtcaaag gagtaaaggc
     6241 attcatgctg gaaatattga taatatttgc tcccgagcga ccaatcatat cctgtgcaaa
     6301 gacctgtgtt ggcaataagg ttcccaaata gttcaaattg aatacaaagt tgatacctgc
     6361 ctcatccaag tcgaagaaag ttttggtatc agctggtagt ccaatctcat ggaattcatt
     6421 gtccgttgtc gcttttggat tattgcctcc agcaccattg attaaaatat ctacaggacc
     6481 aaaatctgct aggacttgct ggcggactcc ttccaaattt tccttagaaa gaacgtttga
     6541 tttataggct tttgctacgc ctcctgctgc tgcaatctca tcaacgaaga cctgagccgc
     6601 agcttcattt aagtccaaaa gcgcaacctt tgcacctgcc ttagcgaatt cttttgccaa
     6661 atagccacaa agaacgccac ctgctcctgt aataacaaca accttgtcct taaattcaat
     6721 aacacgtgtc atactagctc tccttatttc cgactgtttt gactgtaatt ccaaaatctg
     6781 gtgtcttgcc tgctgctcgc atattggctt catagagacc gttaaagtag gttgccccaa
     6841 gggctcggtc atacagacca taaccaggtg tcttggtctg gtctccccag atacgacgac
     6901 cgtggtctgg acgcaaggca ccagtccagt cataatctac caataattta acaacagcgt
     6961 tcatgtcaat gtcaccagcc tgtgacagat gtgctgtttc ttggaagccc caagcccctg
     7021 ctgtcacatt gcgcgtatgc ataaaattga tacgattgcg tttgagagca tattccgtca
     7081 tggcaaggac atcatttttc ggatcagaag cataggatcc gacacacatg gtaattccat
     7141 tgtgctctga atcatagaga ttcaaaaatc gttcaacggc ttcttgacca gtaataatac
     7201 ggggcaatcc gaaaattcca tacggagggt catcaggatg aatggccatt ttgacgccag
     7261 cttcctcagc cgttggtaga atcgctttga taaaatactc caaattcgcc cataaatctt
     7321 cttcagagat attctgacga taattttcaa taatcgcctt catttcttcc ttggaatagg
     7381 acgaatccca acctggtaag ttcaaatcat ccgccacagg atcaacacct gctaaatcag
     7441 atttaaggaa agctaaagaa gttgaaccat ctggaagtgg gtggtgcaag tctgaacggg
     7501 tccagtcaaa cacaggcatg aaattgtagc atacaactgg aatacctgca gcccctacat
     7561 tacggattga agttttatag ttttcaatta actcatcacg atttggcttc ccttgtttga
     7621 tgtcttcatg aacaggaatg gattcaataa ccgtaatttc aagtcctgcc tcttcgacca
     7681 tttttttcag ctctagaatg ttttcaaggg gccaatcttg accaactggc acatcataga
     7741 cagctgttac gattccctgc ataccaggaa ttgccttaat ctcttccagt gtcacaggat
     7801 cctttttccc ataccagcga aatgacattt tcatgattta ctctccttct attgttcaaa
     7861 aaactctttt gcattgtaat aggatatggc ttctacaaat tgccctaaac gattgtaatc
     7921 ttctggcact tcctcatcaa ctatccattg accaacatag gtcgctaaaa tgcgtctgaa
     7981 ataatcatgc cgttgataag ataaaaagct acgcgaatct gtcaacatac caacaaaatt
     8041 agcaagcata ccctgttctg cataggcatt catctggtca atcatgccca acttggtgtc
     8101 attgaaccac caaccagcac caaattgtaa ctgactacga agcccttcct cattggactg
     8161 gaaattcgcc aaagtattgg ccagagcaat gttgtaactt ggattgagat tgtaccaaat
     8221 catttttgga agggcatttt ccttgaccaa gttatctaac aaacgattca aattcacagt
     8281 cagacatgtc tggtctccaa tcgaatccac acctacatct gctccaagtt tttcaaacag
     8341 tcctgtatgg ttgttccgta aggcaccaaa gtgaacttgt gtcacaaagc catgtttctt
     8401 gtacaatcgg cacaattctc taaagatagc agtctgccat ttattgatac tggattggcc
     8461 agcctcttta cctgcaagtc ttgctagtaa aatttcatcc aactcttcga gggtagcctc
     8521 ttcataggaa atagccgtaa aactgatgtc acttgctcgg caaccgtgtt gagcaaaata
     8581 ggaaacgcgc tgaccaagtg cctctacaaa acttgcaaag tctcttaccg ctacccctgt
     8641 tacttcctcc aagcgtttca taaatagatt gaaatttcga tgctcgataa aggcttcatc
     8701 tgggcggaaa gtcggtgcca ctacagtatc tatggtcgag tcctcagcca agcgcttatg
     8761 ccattccaag ctatctagtg gatggtcagt tgtcccaata aaggtcaccc tagcatccgc
     8821 aatcagctta cgtggactaa ctcgatgttc ttgcaaataa gcattgagct gatgatagag
     8881 tcgctctgca ttttcttcgg tcaacaattc ctcaacaccg aaaacctgtc gcaattccaa
     8941 gtgactccaa tgatagagag gatttccgaa agcccgttcc aaggttcttg cccaggcttt
     9001 aaacttatct agtttacttg ctgaacctgt gatttcttct tctgagatac cattagcccg
     9061 catcaagcgc cacttataat ggtcaccccc cagccacaga tcaacaatat cttcataaac
     9121 cttatcttca aaaatttcct taggatccaa gtgacaatgg aaatcataga taggaagctc
     9181 tgctattttc ccgtaaagct ccttagcagg ctcgtttttt agcataaagt ttttgtcatt
     9241 aaaactcatt tcatccctcc aaagctgcaa caaaactgcg agcaatcgtc gttacacttt
     9301 catagccttc agcctgcact cgacttgcca gagccgaacc gactccaaca gcacaagcac
     9361 ctgccttttt ccaatctgca acattttcaa ctgatacgcc accagaaggc atcaaatcta
     9421 cttggggcac tggtccgtga atatccttga taaatccagg ccccaagaca ccaccaggga
     9481 aaagtttgat aatgggacat cccgcctgac tagctgtgac aatctctgtt gcagttgcac
     9541 agcctgggaa gtagaatacc tcagcctctt gcgccaagtc ttgaatttct ttatcaaaat
     9601 gaggactaac caggaaacta gcacctgcat caatggccgc ttgtgccagc ttaagattca
     9661 tcactgttcc cgccccgatg actacggaag aatcgtatga gaactcctct tgtaattctt
     9721 tgataactgt cgctgcattt ggagtcgaaa atgtaatttc aatatttcga atacctccca
     9781 aaatcgcatg gcgagcgatt tctttcgcat cttcttcatc ttttccgcga atcactgcaa
     9841 aaaagtaatt ttcttttaat tgctttaaca tcttgacctc caattagaaa gcgttgtcat
     9901 tcatagctat agtatacaat tcatcagatg atttgtcaat aaaaaagcaa aaaataccca
     9961 gaagaattct tctgggctgt ctatttttta tttctattta tgatcgttga gaccatgcaa
    10021 ggtcatatct ttatcgaacc attcgcttaa aacaacctgt cttactgata taatatgtgc
    10081 taacatgcta tcatatgctg caacaggatg gcgttgcttg atagccatta agatgttcct
    10141 gtgggcctgt aaactatccg atttcatctg acgatttgaa taattttcat tgatcagatg
    10201 aatagattga ttaatgacgg gcatcagact tgtcatagcc agattcccac tcatctcagc
    10261 cagcatacag tggaatttca catccaactc caaatgcttc gtgtctccag cagcaacggc
    10321 ctcttcaata gcaaggacaa tttcttctag tcgagcaatg tcttcgtccg aggcaaactg
    10381 agcgacacgc tccgcaattc tcggttcaag caaataccgc acttcaaaca aatctgtcgt
    10441 caacttcaca cggtctctta ccaaagaaaa acctagggga tcctctgata ctcctttttt
    10501 agagctgata taggttcccg acccttggcg cacctccaga atatttcgag tagccaaact
    10561 tcgaacagct tcacgaacag tcgaacgtcc aacttctaaa tcttgggcca attcatactc
    10621 atttggaagc ttatcaccaa cttcatatcc tctttccaaa atcaattcca ataagcgttc
    10681 cgatgctttt tctaccaagg gtttcgccat gtgcttggtc ctccaataca actttctatt
    10741 tgctactatt atagctcatt tcctcttata atggtagttt ggaatggcag tccaacgttt
    10801 cttgatttct cttacaattt gcttgggttg tctatttctt gagaataatc ctttgtgatt
    10861 tccttgaatg cgaatgatac caaccgtggt ttcaaaatca gcaaaattcc agacttgttc
    10921 acccactaaa ttaggaatag aatcaaagac gcgatggttc atatcatagt aatccacctg
    10981 aaattcctct gtataaggaa tatcccacat cgaatgcaaa ccttgaagag tatctgcacc
    11041 atattccgta atcaaaattg gcttgtcagg ataaagtttt tgccaagtct ccaactcttg
    11101 acggagccct ttctctgccg ccttcaagtc accatgagca acataccaac catagtaacg
    11161 attgagacag acaacatcga ccaaatccat aacctcatcc ttatcaggcg ttgctgtcat
    11221 aatattgacc aaggtcacag gacgcttaga cggatctctg tccctcatgc gctgaatcag
    11281 aggttcaaag taagcacgcg cgcctttttc atgccctgct ggctcattgg cgacaaccca
    11341 catcactacg gatggatgat ttttgtcacg tgctatcaat tcatcaatca ccaattcatg
    11401 agcatccttg gtttgcataa cttcccaggt gctttttacc tcttcagaat cccctgtcaa
    11461 attcaaggct gcattgaaaa gctggaatag gccaactgct ggcacttcat caaggacgac
    11521 aattcccaaa cgatctgcca gccgcatcat ttcctcagaa tagggataat gggaggtcct
    11581 aaaggaattt gctccaatat ctttaagcaa attcaaatcc atcagattgg ctgcttcatt
    11641 gaaaccacgc ccattgatga aggtatcttc atgtttacca aatcccttga aatagaaagg
    11701 ttttccattg atgaaaaact gcccattttc aactgcaaca ctacgaatgc cgaattgttc
    11761 tgagtaggta tctagcaagt tttctccatc aaataattca actttagcag tatagagata
    11821 ggcatccaag acctcccaaa ggcgtggatg ttcaatgacc atttgtccat tttcaagaag
    11881 acctacttcc tgtccctctt gatctaaaat agacatacgg atggaatcgt actgcccaac
    11941 tgtcttcaca tcaacattca ctaccgcctg agtcaaatct tgatttagct ccgtccgaat
    12001 aacaatatcc tcaatcctat tttggggacg tttatagaga tgaactgtcc gctggatacc
    12061 agcatagttg aaaaagtcaa aattttcctt gacggtcttc acaatttgac cattctcatc
    12121 cacttcttcg ctataattcc ctactgggag ggttgtgtaa ttcaactcat tattagcaca
    12181 aaccgtcaaa cggaattgat cagcgtcgta taagtttgac ggaatcaagc attcaaaagg
    12241 tgtgaaacca cccttgtgct ccccgattaa ctgaccatct gcatagactt tggcctgatg
    12301 tgtcaccgaa ccaaagcgca caaccaactc ttcatcagct gatacgacag ggagagctac
    12361 cataagttca taccaattat ctccgacaaa acgacgtttg tccttatcaa caacaagatc
    12421 attaaaagaa ctcggtacaa ccattacttc atctgtatct aacagagtat gaacatcatt
    12481 gttcccttgc ttaaatttcc atattccatt taaagaataa actgagcgtg tttttgtctg
    12541 tattggatac aacatacttt ttccttcttt ctatcgtttt atttcacgtg accctttttt
    12601 caatagcaaa tcaatatctg attgactgat gacattaatg tccccttcaa tggtatgttt
    12661 gaattgaaaa ctagccatac ctaagtcgag tatttcccgt ggagattttt cctgcaaata
    12721 gcccaaaata attcctgctg taaaggcatc ccctgtccca actcggtcca gaacttgaat
    12781 ggattctttt tccgtttcat aaagaatacc atcttgatac aaatatgctt tcagcaaata
    12841 ttcattattg taggtcattt ccctctgggt aaaagcaaga ccctgcaaag aataagtctc
    12901 cgccagacct ttaaccaccg ctaacaagac ttctttattt tcataagggc gcttcaaccc
    12961 caattcatcc ttcagatctt ttccagattc attgaagagt tgaataggct ctagaccaaa
    13021 gcaaatatct gctaaaccta caaaaggaga taatttctcc ctcgcttctt caaaagaaga
    13081 ccacaaactt tctcgataat tcaagtcaaa ggaaacctta acaccctgac cctttgccat
    13141 ttccatcaaa cgaaaagcca gtcgataaat atctggattc aaagcgaccg ttataccact
    13201 gacatgaaac caatcgacac cgtcaaacat gccttccaaa tcataatctt cttccttgga
    13261 taagctgaaa gcagaataat cacggtcgta ggtcacctga ctgggcctga gcgaaaaacc
    13321 tttttgatag taataaagtc caagccttcc ctcgtttcga atcacatatt tctgtccaat
    13381 ctgccgtgca aagagaaagt gttctgccat ttttcccact tcattcttag gcagtgccgt
    13441 caccaactca actgaatgcc ccagctgagc caagctggct aaaacattca actccgaacc
    13501 accaaactga caatctaaac tggtagcctg tgtcaaagtt tgatactgcg gaggagatag
    13561 ccgcaataaa acttctccaa atgctaagat ttttttcata ccgcctccta gatgaaagac
    13621 ccagctggac tcctccaact aggtcgttat tgtattattt atcgtcgtat tgatcaacct
    13681 tatattgagt tacatcaaca taatccatat cagataatgg aacatttttc gcgattgcac
    13741 gcacacgttc ctcatctttt gccgctttca taacatggat tttctcttga atacgtgcca
    13801 tttcttcacg gtctagtttg tagaatttca tcaagaacaa ggatacgctc aataccgcgg
    13861 ctggaatcac tgtcaatagg acaatcagtg ccattctcaa atctgtagag aatgccgttt
    13921 caacggttgg gtactcttta ccaaaaccga tacctgctaa tacaaatcca acaaccattg
    13981 gggcaaatga agaagcaatt gagtctgtca atgagaagat agtcccaatc atacctgaca
    14041 agtaacgacc agatactgaa gtttcatagt ctgtaatatc cgcacccatg gtcaatacca
    14101 aaccagatgg agcactagat gcgtagcgag caacgatata aacaacaaag aagccgattg
    14161 tgtagagatt ccaatttgtc aaatccaaca tacctggttt agctgaaaag agcatcgctg
    14221 ccaagatcac aagaccagca atcccaagtt gaagcgctgt aatataggca aaacgtaaac
    14281 ctttacgacg agccaattgc gcagcaccaa ctgttacaag aatacctggc aaaatcaaaa
    14341 gcatagacat ttttccagaa agagcatagt caccgaagat aataccatac agcataaccc
    14401 cgataacgga atctccaaac agttgagcaa caaattttac aaaagctgca gagattgaaa
    14461 gaacctgcaa aggtttgttt cctttgataa ctttccagta gtctttcaac ttagtttctt
    14521 gtgtcttctc accaagacca aagtatttct tattgtcttt ctcccaaata ccgataatag
    14581 caagaatacc cagaacagct gaaattacta ctacaccgag gaacaattcc tggaagaaac
    14641 ttggagtgaa accaccatgt ttcggaacca atgtccctga cacaacaacc tgactaccag
    14701 tcatcaacac agttgtcgca ataccatcaa caatattaaa gattggacgt tgctttggat
    14761 cattggtaag cgcaacttgt cctgcttttg taatcgtctg ttgaagtgaa tatccaattt
    14821 tatggataat caaaacaagg atgaatagag gaagacggag actctcatta actccactta
    14881 aacctaacat cataaaaaga gaagctgctg taatgatatt ccccaaaacc aagataggac
    14941 gatatttacc aaacttagta tccgttttat caatgataac tccgattgta ggatcaatga
    15001 aaccatcaaa gatacgaata tagcccatga gttgactaac aaacagggct gcaagaccaa
    15061 gcacacctgt cgataaataa gtcacgaaag taaaagcaac caagtaaata ttcgtcgatg
    15121 aattgttcat cgcaaataat atgatttgcc ataacttggc tttgttataa gtaacgtctt
    15181 cgacacgttg ctcaacatgt ttttccattg gaaattcctc ctaaatgttt tctacgacaa
    15241 agtcgtaagc gttttcttta actatattct aatattcatc agatgatttg tcaactgttt
    15301 ttgtaaatat ttttaattat acgcaaaaaa agacgatttc acgtgaaaat cgtctgatat
    15361 atttgattta gcgaggaaga attcatctga ctatttattg atttactgca atttccccac
    15421 tatgatagtt gatactgaga ccactctgca catttggaac gaacacattg aactctaggc
    15481 taccatctgc tgcttttgcc tctagatggc ttccgacagg aacaaggtct tcactctcag
    15541 catacaccaa ggtcacacga tagcggatac gcttattttt atccaaggcc cgccgaatct
    15601 gactttcaaa ataattctgc ccagttgaag tttggtcgtt agcttggttg gaccaagctg
    15661 tttgaactgc aatattctta ggattgctgg ttgaagcatc gaagccagac aatccaccaa
    15721 tcaaagcata acctaggagg tgaccacggt ccactgcatg gtcatattct ccagttaaat
    15781 catggatctg atgccaaccc gcaggtgtcc aagaagttcg accatttcct gtttcttcac
    15841 gatccttata ctgacgtgta gatttagcta aaagcgcatt ggctactgtt ggaaccgttt
    15901 ctccctggac tgtctttgtc ttattatcac cataaggtac actggcaacc gatgcatcta
    15961 aatctgtttg attaccgtta ataataaatg ctccagcgcc attccattca atcgaattcc
    16021 ccagttgctt ttttacatct tctgtcaata ccgaactagc cagttcctga ctaggtgtgc
    16081 cttcacttgc cttagactga ttgacataat aggaataatc agcaacgtta ctgctcttgt
    16141 tatcacttaa aagatagcca cccgctacga caacaatcgt agccaataag ctgagcagac
    16201 tcattgcttg ttttcttaca tttttcttcg ccattttctc tccatcaaaa aagcaggtcc
    16261 agcctacttt cctgtaaatt taccgattag ttcttgccac ttagctggtg acaacaccgc
    16321 aaatacattg tctccatctc ctatgacacc gtaaccaacc attaatccta aaacaaacac
    16381 caaaatagca atcaacaaaa caactgctac taagattaaa tttttaccaa catagctaag
    16441 attatcctta tccattttca tcttctccac tcacacgttc aatcgttgca cctaaagcag
    16501 ctaatttctc atgaaaacga tagtaacctc gatctaagtg cgttaattta cctacaatcg
    16561 tttcaccttc agcaaccaaa ccagccagaa tcaaagcggc acttgctcgt aaatcagtag
    16621 acataacctg agcaccttgc agagcaagtc ctcccgtgat gatagctgta tctcgtaaaa
    16681 tttccgaatg caaatccatc cgacgcattt cttccaaatg ctggaaacga ttttcaaata
    16741 ccgtttcaat catggtagat tcaccctttg caactgccat tagagctgta aattgagcct
    16801 gcatatccgt tgggaatcct ggatggggca aggtttttac tgttacaggt ttaagcttat
    16861 ccacttgcga acgaactcga atcccttcag cttcttccgt cacctcaact cccatttcca
    16921 acatcttaga aatgagtgga cggttatgct cccagacagc gtccttgacc aagacatctc
    16981 caccagtcat tgccgctgcc accataaagg tacccgcttc gatacgatct tgcaccactt
    17041 gatgttctgc gccagtcaat tcttctacac cagtaatcgt aatcgtctct gtacctgccc
    17101 ccttaacttt tgcccccatt ttgtttagga aaatagccaa gtcaacaatt tcaggttctc
    17161 gcgctgcatt ttctaatacc gttgtaccct gcgccagagt tgcagccatc atcaaatttt
    17221 gcgttgcccc aacaccaaca cttgggaaat caagatagat atgagctccc tgcaagttct
    17281 ctgcactcgc ttcaatataa ccagctcgtt gtttaatttt agcccccata gcttgcaaac
    17341 cttttaagtg caaatcaatt ggacggctac caattgtaca acctcctggc atagaaacct
    17401 tagcataacc attgcgtgcc aaaatcggac caagaataac aatagaggcg cgcatttgac
    17461 tcacatactt ataaggagtt tcgctaccaa gtttacctgt tgcgtcaata tcgatagtct
    17521 ttgtttcctc gtcaaatttc acacgaactc ccagaccacg tacaacatta ttcattgtat
    17581 agacatcaga caaaatagga acctgagtca atttggtttg cccttgactt gctagaactg
    17641 ttgctgccaa taaaggcaga acggcattct tcgccccctc aatctcaacc tgtcccttca
    17701 aaccgttttt actacctgta acaataattg tatccatttc ttatcctttt cttatcttaa
    17761 atcacatatt tgcaaaagag cgtcccacca tcaacaattc taaaaagaaa gtcgagacta
    17821 agtaccccag tgctattgcc agaaagagaa tcagcaaatt gattttacgc tgattatccc
    17881 ccgtcacctt caaccaccgt gaccaatcaa ctgttgtaac caagagatga tgagccagat
    17941 agataaacaa tatatgacta aaaaactgta aaattgaaat taccatgttt ccattatacc
    18001 atgaaattaa aaaatggaac tgaaccaagt tcaatcccat atagattaat gtttcccaac
    18061 acgaatacga tttatcgcac gttggagggc gactgttgca cgcatctcca tatcaatatt
    18121 acgagcagtc tgtgcttctt ccaattcacg ttccgcgcgc aatttggcac gttctgcacg
    18181 actaatatca atatcccgtt cccgctcagc ggaatcagaa atgaccgtaa taatatcctt
    18241 gttgacttca atgattccac cattgaccgc aatccagtct acatggtttt catcatcaac
    18301 acgacgcact ttcatttcac cgatttccaa aactgcaatc aattcttcat gtccagaata
    18361 gacacccatc tcaccatcaa ccgttttgac caaaacaaat gctgcatgat ggtcatattt
    18421 gataccgtct ggtgttacga tttgtacagt catttgtccc ataagccacc tctagaattt
    18481 cattttagca gctttagcga ccacatcctc aattgaacca acatttcgga aggcatcttc
    18541 tggtagatgg tcgtgtttgc cgtccaagat ttccttaaag cctttcaccg tttcagctac
    18601 tggcacataa gaacctggca tacctgtaaa ttgctccgca acgttgaagt tttgagagag
    18661 gaagaattgg atacgacgag cgcgaccaac caaggtcttc tcttcatctg ataattcatc
    18721 cataccgaga atggcaataa tatcttgcaa ttcttggtaa cgttgaagga cacgttttac
    18781 ttccatagct actgcatagt gctcttcacc aacgatttgt ggagcaagag cacgtgatga
    18841 cgaagccaat ggatccaccg cagggtagat accaagctga gtcaacttac gctccaagtt
    18901 agttgttgaa tccaagtgag cgaaggctgt cgctggagct gggtctgtat agtcatccgc
    18961 aggtacgtaa atagcctgaa tagatgtaac agaacccttc ttggttgagg taatacgctc
    19021 ctgcaattgt cccatctcag ttgcaagtgt tggctgataa ccaacggctg atggcatacg
    19081 acccaagagg gcagacactt ctgaaccagc ctgtgtgaaa cggaagatat tatcgatgaa
    19141 cagaagaaca tcctgccctt ccacatcacg gaagtattcc gcaatggtca aaccagtaag
    19201 agcaacacgc atacgggctc ctggtggctc attcatctga ccaaatacca tggccgtttt
    19261 ttcaataaca cctgattctt tcatttccca gtaaagatcg ttcccttcac gggtacgctc
    19321 gccaactccg gtaaatacag agataccacc gtgttcttgt gcaatattgt gaatcaattc
    19381 ttggataagg acggttttac caacaccggc accaccgaag agaccaacct taccaccttt
    19441 tagataaggt gctaggaggt cgataacctt aatccctgtt tccaaaattt cgcttgaagt
    19501 agataattcg tcaaaagccg gcgctttctt atggataggc tcacgttcaa aatctgccgg
    19561 aaaagactct tcaaggtcaa tggtatctcc caacacattg aagacacgcc ccagcgtttc
    19621 tttaccgact ggaacagaga tgggacgacc tgtatcgaga acttccatcc cacgtgtcaa
    19681 tccatccgtt gattccatgg caatggtccg tacaacgcca tcaccaagtt ccaaagccac
    19741 ttcaagcacg actttttgtt tggaatcatc atttttatat acaacgagtg cgttgttaat
    19801 ctcaggaagt ttatcttctg ctgcaaacgc aacgtctaca actggtccga caacctgagt
    19861 aattttgcct gaactcattc tatttcctct atttctatta ctcaagggct gaggcaccag
    19921 ctacaatctc agtaatttcc tgagtaatcg ctgcctgacg agccctattg tattggattg
    19981 tcaaatcgtt aatgacattc ttagcattat ccgtcgctgt ctgcatggct gtcatacctg
    20041 ctgcattttc agcagttttg gcatccaaaa tcgctccata aatagttgat tctgcatatt
    20101 gagtcaagag ttgttccaaa ataacctcac ggtttggttc caactcaaag gtagctgtat
    20161 aaccatctgc ttcattgtgg tccaaatctt ctacaggaag catctgttgt acacgcacct
    20221 gactggtcaa actattgaca tggtggttat aacaaacata caactcatca aagagttcat
    20281 tcttgtacat ttcaacagac ttagaaatga ttttttgaac ctcttcaaaa cttggattgt
    20341 ctgctaaacc acgcaattca aagacaggat tgataccacg agcacggaag aaatcagctc
    20401 ccatactacc aatggcaata atctcatact catcttttga gtcatggtcc tgttcaatca
    20461 tccccatcac agccttcaaa atactggaat tataagaacc ttttagtcca ctgtcagagg
    20521 taatgacaat ataaccagat ttttgaactg gacgacgaat cagcattgga ttgcttgtat
    20581 ctgaagccat caattctcca cgcaagagat ccgttgtaat ctgacgaacc ttgctagcgt
    20641 agatttgaaa ggattgcgct aattgctctg atttggctag tttagaagcc gaaaccatct
    20701 gcatggcacc ggtgatttga ctggttttct tagtagatgc aatttttgac ttgatttcat
    20761 tgagagaacc tgccattatt cactccttta cttaaagaca gactggtctt tgaaagcttg
    20821 gatagcagca ttcaattcat ctgtatcagg aagatctttt gttgtacgaa taacatccaa
    20881 aagaccatcg tagtgcaaat caaaatatga gtacagttct tcttcaaatg ccaaaatatc
    20941 gtcaattggc actgaatcca agaagccatt tgtcaaagca tacaaaatca aaacctgttt
    21001 ttcaactgga agtggcttat gcaatggttg tttcaacact tccacagtcc gacgaccacg
    21061 gttgagtttg gcctgagttg ctgcatccaa atccgaacca aattgcgtaa aggcttctaa
    21121 ttcacgataa gaagctaagt cgatacgaag ggtacctgcc acctttttca tggccttaat
    21181 ctgtgcagag ccacctacac gtgatacaga agaacccgca tcaatggctg gacggatacc
    21241 agagttgaat aaatcgtcct tcaagaagat ttgtccatcc gtgatagaaa tcacgttggt
    21301 cgcgatgtaa gctgaaatat ctcccgcttg tgtttcgatg aatggtagag ctgtaatgga
    21361 gccaccgccc aattcatctg aaacctttgc tgaacgctct agcaaacgac tgtgcaagta
    21421 gaatacatcc cctggatagg cttcacgacc cggaggacga cgaagcaaga gggataattc
    21481 acggtaagct accgcttgtt ttgacaaatc atcgtaaaca atcaagacgt gtttgccttc
    21541 atacatgaac tcttctgcca tcgcaacacc agcataagga gccaagaaaa gcaatggaga
    21601 cggttgtgaa gcagatgccg ttactacgat tgtgtaatcc aaagcaccat attgacgaag
    21661 ggtttcaacc tgagtacgaa cagttgactc tttctgaccg atagccacat agatacaaat
    21721 catgtcttgg cctttttggt tgagaatagc atcaatagct acagatgtct tccctgtctg
    21781 acggtcaccg ataatcaatt cacgctgacc acgaccaatc ggaaccaaag cgtcaatagc
    21841 cttaagacca gtttgaagcg gttcattaac cgacttacgt tgcataacac ctggtgctgg
    21901 atattcaatc ggacgagtct tactagtacg aatttcacca agaccatcca ccggttgacc
    21961 aagtgggttg atgacacgac caatcaaagc cgaaccaact ggtacttcca taattttacc
    22021 agtacgacga acaactgacc cttcacgaat atcagtaaat tgtccgagaa tgataatacc
    22081 aacatcgttg gtttctaagt tttgtgccat accaatggtg ccattttcaa ataccaaaag
    22141 ctctccactc atggcattgt caagaccttg cgcacgggcg ataccgtcac cgatataggt
    22201 tacgacacca gtctctgtgt agtcaaagtc aggctgaaag ccttcaattt gttgttttag
    22261 taaagcgcta atttcttgtg cattaatcaa aagaacacca ctttctattt tctaaagttt
    22321 ttttctaacg tccttcaact gtgtccgaac gcttgcatcg attaccttat ggttaaccgt
    22381 tacaataaaa ccaccgagga gactttgatc aagttcttcc gtgatattgc gcacacgcaa
    22441 ggaaaatcgt tgttctacca agtgacggag acgctctttt tgagcttctg tcaacggata
    22501 gactgaaacc acacgggctt caaattcatt tttaaattta ctgatttgta attggacacg
    22561 ctcaagggtt tcaagaagca agtctgcatg accatcacga ataacatctt caatcaagtc
    22621 attgatttgc cagaaactag attgacgtac cgtccttaca aagtcagcct tttcttctct
    22681 cgatactgtc gctgacagca agattcgatt cagcttacta tcatgaatga tggaaatcaa
    22741 gtcagaaatc tggtcataca tatcccaaat atctgcatga tcactgactt tttcaacaaa
    22801 tgatagagca tatttttgca cgatggcatt ttctctagcg ttcatctact tgtctcctag
    22861 tttgtccaaa taacggtcaa tcaagttact atgagcttgt tggtccaaat cttccaaaat
    22921 aattttgctt gcaagatcaa ctgccaattc agcaacttga acacgcaagt tttcttgtgc
    22981 ttcacgtttt tcagcttcaa tttccaattg agctttttgc ttcaatcctt gaatttcaac
    23041 atctgcttgc tcaagaatgc gttttttctc tagttttgcg cgttcaaccg cgtcttgaat
    23101 aatcttttga ctttcaatac gtccctgtac caactcatct tcacgttgtt gaacaaggtt
    23161 tgctgctgct gttaattttt cttctgcagc atcaatatcg ttcgcaatct tattggcacg
    23221 ttcttcaaaa atacctgtaa ttttatccca tgcaaaaaca cgaataagaa taataagtac
    23281 tgcaaatgag gctgttacca agataaagtt tccaagaatg gtactagatt gcattgtaat
    23341 agttgttgcc atagttgtct ttccctttcc tattcactct cactactgat ttttttattt
    23401 aagtacatag ataccaacat gataaataca tatccctgca agcatgaaat gaatacagaa
    23461 aatgctgtcc aaacaacgtt cagagcaaag gcaattggat atgcaagtgc actttgttga
    23521 gataattgta gaagaagaga gacaagcact tcaccagcat aaatgttacc gtacagacga
    23581 agagcaagcg acacaaggtt ggtcacttct tccaaaatgt tcattggagc catagcccag
    23641 ggtgtcacaa aagatttgag gtatgccttg aaaccgcgac gacgaatacc ctcaacatga
    23701 caaaatactg ttgcaattgt tgccaaaccg aaatcgtaag ccatattggc cgttggagaa
    23761 gtccacaaat tatgtccttc tgctgtttcc aatttggtca tcaaacctag attattggca
    23821 accaaaagaa aagtaaagac tgtaaagaag aacaaggaat accgctttgc ttccgcatcg
    23881 ccaagattcc cttttgtaaa attgatggtc aattcgtaaa cgtactccaa aacattctgt
    23941 tttccttttg gcttgagctc catccgacga ctagcccaaa aaacaagtaa gaaaataatg
    24001 gaaatggtaa tgacagaaac caataccatg gtcaggtcaa aggttacggg accaagggta
    24061 agggttggac ttaaatgttc ttccaatgga ggttcccccc ttttctattt ctctattttc
    24121 tactatttca agacaaatgt catagccaag gttacgaaga aagttccctc gataaaggca
    24181 acacccaaaa atacaccagc catcaattta ctttgcattt ctggttgacg tgcagtcgaa
    24241 ctaaggtatg aagccaccaa cagtccctca ccgatactta caccaagaca ggccaatcca
    24301 agggccaatg cgcctaaact cataacgaat tctcctttta aaatttaata ccatgtaagg
    24361 atactcctaa aacatctatt tgtcaagaaa ataccgccat tgtaagcgtt taattcgttt
    24421 ttgtaaaata aaaagaaaag agtatttcat cttttcttcc tatttaatag cttcatcaat
    24481 atggtctgtt tcttcaatga atcgtttggc catatcttct cgattcatct gatcagtatc
    24541 aagagtgatt gcatggtcag aaaccagatc tgtatctgcg aaaaactcta tatggtttga
    24601 taggttgtgc agacgtacag gggcttcccc accaaactct ccatctagat tgagcagtag
    24661 acggttatct gggttcagat tttcgatgga taggctcttg gtttttatgt attcaactaa
    24721 atcactctca atgtgtttgc caccgtctaa gacttgacga atcagatgca gaatttcaaa
    24781 tagattccct gttttaacca tcaataaggt aaaattacca tcatctaact tagcatcggg
    24841 aacaatctgt tcaaaacctc caattgaatt ggttaaggcg acaaaaatca ttgaaaccga
    24901 gccttcaaat actccctcgt catgctctac acgaacaggt gtaaattgta cttgtggcag
    24961 gagttctgct ccttttacta cataggccaa atagccaaac atggtcttaa gttgactggg
    25021 aacactgtag gtcaactccg taagtgtacc tgccgcagca atattgataa aataatgttc
    25081 ttgatgaaaa ccattttttt gatttttggc taaaccaata tccattaaaa tggtctgctg
    25141 cttaccgata accttagctg cttctacagg atttccccta ggcactttta aggcacgcgc
    25201 atagtcattg gtggtgccag tcggtatgat ggccatctgc ggacgttttt caagcggagc
    25261 aattccattg acgacttcat tgattgttcc atctccacca gccgcgataa tcaagtcaaa
    25321 gtctgcaagc gccgcacgtg tagcttcatt ctttgcagag tctttctcag cagttgtttg
    25381 gaaagctgaa gtttcataac cgtagccttc caaaatttcc aatacttcgg ccacattttt
    25441 cttcatgatt tcttgacctg aggtcggatt atatattagt ctagcacgtt ttctttcttc
    25501 catcgtttta cctttacaag ctctctaacc aagcctcatc tttaatttcg atacctagct
    25561 cctgtgcttt ggtcaattta ctgcctgcat ctgttccggc aaccacaaga ttagtttttt
    25621 tagaaacaga tcccgccaca tttgccccta aagcttctaa tttggctttg gcttcgtttc
    25681 gtttcattcg ctccaattta ccagtcagta caactgtcat acccgatagc gcagcatttt
    25741 catctactac ttgtccgaga taggttaagt ttaccttagc ttccttaagt tcggctaaaa
    25801 gttgttgcgc accctcacta gcaaaaaaag ttgtcaaaga cttagcaata acctgaccaa
    25861 gaccttctag cgaagcaatt gcttcttgat cagcaaatgc tagagtttct aaatcaccaa
    25921 atttttctac taaaatctta cttgccttac tacctacatg gcgaatacct agtccaaata
    25981 ggaggcattc tgctgagtta ctcttagaag tttgaatggc ttgatagagc ttgtcagctg
    26041 atttttcttt gaaaccttcc aaggtcaata ggtcatcaac agttaattta taaatatcag
    26101 caacatcgtg taccaagcct gctccaaaga gtttttcaac gattgaagac cctagccctg
    26161 cgatattcat agcatcccga cttgcaaaat gttccaactt actcatcagc tggctaggac
    26221 aaatagggtt gatacagcgg agggcaactt cgtcctcata gtgttgtagg tcgctctgac
    26281 aagacggaca atgactagga atgggcatga tttcttgctc ttttcgatac ttgtccacca
    26341 ctttcagaac tgctggaata atatctcccg ccttataaac aatgaccgta tcgccaatac
    26401 gaatatcttt ttcggcgata taatctacgt tatgcaaagt tgctcgactg acagtcgttc
    26461 ctgccagttg aacaggacta agattggccg ttggtgtcac aacacctgtt cggccaacag
    26521 tccaatcaac cgacaagatt tctgcttctt tttcctcagc tggaaacttg taagctactg
    26581 cccatcgtgg tgctttaact gtaaatccta gttcttcttg tatagctaaa tcatttacct
    26641 tgatgacaac accatctatc tcatagggta aatcatctct acgctctgct attttttcaa
    26701 taaaatccca cgtgtcatca atcgactccg ccaagcaata ttcgtggttg gtcacaaatc
    26761 ccagagcatc cagtttctcc agtacttgtg actgactagt cgcttcagat ggactagcct
    26821 cttggtagag gaaagtagcg agaccacgct gggccaccac tcccgtatca agctggcgga
    26881 gggtccctgc tgctgcatta cgcggattag caaattcagc ttcaccagcc tcctgacgct
    26941 gtttgttaac acggtcaaag gaagcttttg gcatataaca ctcacctcgg acagtgatgt
    27001 ccacagcttc gggtaaggtc aaaggaacat cagccactcg tttgagattt tctgtgatat
    27061 tttcaccgac actaccgtca ccacgtgtag cacccacgac taaattaccc gcttcatagg
    27121 tcagggaaat agacagacca tcaatcttca attcacagat atacgttgct tgaggaaatt
    27181 ccttgcgaac tcgctggtca aaagcttcta actcttcacg agaaaaggca tcctgcaaac
    27241 taaaaagagg ataaacatga ctatattttt caaatccgtc tagcatcttt cccccgaccc
    27301 gatgggtagg actatctggt aaaatcagtt ctggatgagc cgtttctagc tctactaatt
    27361 cacgataaag cgtatcatat tctgcatctg atacgctagg ttggtccaat tgataatact
    27421 ctttagcgta ttgattaagc acgctaacta attctgatat tcttgttttc ataagaatat
    27481 tatatcatga tttgagggtg ctaactatat ttgcgcttca ataaaaac
//
LOCUS       DASFSW010000009        26916 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.009, whole genome shotgun sequence.
ACCESSION   DASFSW010000009 DASFSW010000000
VERSION     DASFSW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26916
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.009"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..732)
                     /locus_tag="U1099_000343"
     CDS             complement(<1..732)
                     /locus_tag="U1099_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM4866693.1"
                     /translation="MFLHVKYIKEMFVKKRTLLFLSLCASALNAQLVQADMVNAPSSQ
                     VSSSQVAETGGQLSVVDEAGQIKAILSNIQGEIIGVTAQFSTETNSGITVTFSMDEQG
                     RYVAVLDRSAFAEEDQVFSLKLTAQLTDGSFQTLSDYSFEWKKDVVSAAEKPTALDNS
                     DATPSKIEEEATSTTTSSTVNSSLTAGATSSTSNSLTRSSSNTIAGSSNGSASVPSAR
                     VSTASSSVNVTQPTGTITIENRNDAQ"
     gene            complement(770..1810)
                     /locus_tag="U1099_000344"
     CDS             complement(770..1810)
                     /locus_tag="U1099_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4866694.1"
                     /translation="MKKKAIVKPLLMSGIFLALLGSATLPSGAVPVVAAETSQSTTYH
                     LTDDEKVAVREYIQAKMTIDMQEYRLAFLEGMMEEMASGSAEAAWDEEIADLKANLTA
                     EQVVVLDELEANLIGSIAQHYHYLFETLTVAGKSGREEAAAIVSKYESEDDASTPEAE
                     LAALKYAREVIVELLNKESAAIDNYIAYAEATGQELAGLLESGNSNLESITSATIGYG
                     QALATASQPKFPYDFSEMDRQIAELTASLQSKVEDKSTAKTENTGVQTSQSATNGSNE
                     QDVPGQGGGQISDVTTGKGNISEAGQKKVIPNDNAKVLPKTSGKSSLPLTVLGLITIF
                     AGWLLTKKQEEK"
     gene            complement(1827..2678)
                     /gene="rfbD"
                     /locus_tag="U1099_000345"
     CDS             complement(1827..2678)
                     /gene="rfbD"
                     /locus_tag="U1099_000345"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019780653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEM4866695.1"
                     /translation="MILITGANGQLGTELRYLLDERGVDYVAADVAEMDITDADKVDA
                     FFAEVKPSVVYHCAAYTAVDMAEDEGKELNYKINVTGSENVAKAAAKYCATLVYISTD
                     YVFNGDLPVGKEWQVDDQPDPQSEYGRTKRKGEEAVEKFADKFYTVRTAWVFGNYGKN
                     FVFTMQNLAETRDTLTVVNDQHGRPTWTRTLAEFMVHLVDTKQAFGYYHLSNDAAEDT
                     TWFDFATEILKDTNTKVLPVDSSQFPAKAKRPFNSTMSLDKAKATGFVIPTWQEALEA
                     FYKQEKK"
     gene            complement(2733..3779)
                     /gene="rfbB"
                     /locus_tag="U1099_000346"
     CDS             complement(2733..3779)
                     /gene="rfbB"
                     /locus_tag="U1099_000346"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEM4866696.1"
                     /translation="MSQFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     KANLEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETNYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRMGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWTPQFTDFSQGLEETIQWYTDNQDWWKAEKE
                     AVEANYAKTQEVIK"
     gene            complement(3967..4452)
                     /locus_tag="U1099_000347"
     CDS             complement(3967..4452)
                     /locus_tag="U1099_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEM4866697.1"
                     /translation="MFQAFLEIAEQLNKLGITPLLMGSVGLERRTGRDWQARDLDIHV
                     PSDPRGWEAPDEERIYRADELIVLMNQLGYVLVDRHEHEFHKDGLSVEFGGLHSLPEF
                     AGVELEDLEELETVGVRYYLPTLEQYLKIYQASSKDSYRAENNNQKDFAKIAYLKEIL
                     R"
     gene            complement(4452..5045)
                     /locus_tag="U1099_000348"
     CDS             complement(4452..5045)
                     /locus_tag="U1099_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEM4866698.1"
                     /translation="MTENFFGKTLAARPVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGTWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDFVAYSYLVNDYWALELKPKYAFVNYA
                     DPSLDIQWENLEEAEVSEADKNHPLLKDVKPLRKEDL"
     gene            complement(5045..5914)
                     /gene="rfbA"
                     /locus_tag="U1099_000349"
     CDS             complement(5045..5914)
                     /gene="rfbA"
                     /locus_tag="U1099_000349"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEM4866699.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDNV
                     ALILGDNIYHGNGLTKMLQRAASKEKGATVFGYQVKDPERFGVVEFDADMNAISIEEK
                     PEEPKSNFAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNVQVANLEEIAYRMGYITKEQVYELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(6012..6836)
                     /locus_tag="U1099_000350"
     CDS             complement(6012..6836)
                     /locus_tag="U1099_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEM4866700.1"
                     /translation="MTWVTNQSVVLQALLGGLFTWFCTIMGSAVVFFFKTVSRRLLDT
                     MLGFAAGVMIAASFWSLLAPSIEYAESSYGNLAWIPAAVGFAAGGIFLRLVDAWVPHL
                     HLGNDKDKAEGGGEKDRKNLSKTALLFLAITIHNIPEGLAVGVTFGALASNYSPAAFI
                     GAIGLAIGIGIQNIPEGAALAIPIRTDGASRWKAFYWGSMSAIVEPIAAVIGAFAVTF
                     MTPILPYALSFAAGAMIFVVVEELIPESQTNGNTDIATLGLMAGFIIMMILDVALG"
     gene            complement(7012..7806)
                     /locus_tag="U1099_000351"
     CDS             complement(7012..7806)
                     /locus_tag="U1099_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEM4866701.1"
                     /translation="MLASQVIERYQAFCPPSLAMEGDVKGLQIGTLNKEIQRVMVALD
                     IRETTVAEAIDKKVDLIIVKHAPIFRPINDLVADNPQTKMYLDLIKHDIAVYVSHTDI
                     DVVEGGLNDWFCELLEIQDTTYLIETAEGQGLGRVGTIAEQTLEELALKVKSVFGLDA
                     VRLVTYGHQAKQYVSRVAICGGSGGSYYHEALAKGADVYITGDIYYHTGQDMLTQGLL
                     AIDPGHHIEALFIKKIAELLEIWKAKEGWDVEILPSTVSTNPFRHL"
     gene            complement(7796..8479)
                     /locus_tag="U1099_000352"
     CDS             complement(7796..8479)
                     /locus_tag="U1099_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEM4866702.1"
                     /translation="METKLSKRLETVASFVPQGARLVDVGSDHAYLPLFLVEQGRIDF
                     AIAGEVVQGPYQSALQNVEQAGQTEKILVRLANGLGAFEKSDQMTAVTIAGMGGRLIA
                     EILEAGKEKLATIERLILQPNNREDDVRRWLEKNGFRLVAEKILEENGKLYEVLVAES
                     GQMRLSEVEHRFGPYLLEEASPVFVKKWRRELEKLSFALEQVPSERQGDRFAISQKIQ
                     QIQEVVHVS"
     gene            complement(8481..9155)
                     /locus_tag="U1099_000353"
     CDS             complement(8481..9155)
                     /locus_tag="U1099_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEM4866703.1"
                     /translation="MNYSQQFRQGHLVLPAAILFHYQELFPSADDFLIWQFFLYQNSS
                     AIESLAPSEIAQATGKTVAQVNQAIENLQDAGLLEFKTISIAGEIEMIFDALPAFEKL
                     DVLLTPKQAVEIVQPENDLKTLVGDFERELGRFLSPFEIEDLQKTIEDDKTSIDLVRA
                     ALKEAVFNNKTNWKYIQAILRNWRREGITTVAQVEAKNAEREIQTPKNVTVSSDFLDA
                     MDLWKD"
     gene            complement(9165..10106)
                     /locus_tag="U1099_000354"
     CDS             complement(9165..10106)
                     /locus_tag="U1099_000354"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEM4866704.1"
                     /translation="MPIKIEKSLPAVEILRKENIFVMDSDRASHQDIRPIKILILNLM
                     PQKIVTETQLLRLLANTPLQLEVEFLYMASHESKNTHSDHLKQFYKTFEQVRDSYFDG
                     LIVTGAPVENLPFEEVDYWQELTQVFDWSKTHVYSTLHICWGAQAGLHARYGVPKHSM
                     SRKLSGVYCQEVTNPTSPLMRGFDDEFRSPHSRYTEVREADIAHLSGLTILSQGPEVG
                     LSILASKDLREIYSFGHLEYDRDTLAKEYQRDCIAGKNPHLPENYFKEDDPTSKPSLS
                     WNLPAAQFFTNWVNYAVYQETPYDWHIFEQSAQSASL"
     gene            complement(10210..10722)
                     /locus_tag="U1099_000355"
     CDS             complement(10210..10722)
                     /locus_tag="U1099_000355"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEM4866705.1"
                     /translation="MNLKDYIATIPNYPKEGIEFRDISPLMADGNAYSYAVREIVQYA
                     TDKQIDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELDDLKGREAIG
                     DYDYKVLMHY"
     gene            complement(10776..11906)
                     /locus_tag="U1099_000356"
     CDS             complement(10776..11906)
                     /locus_tag="U1099_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEM4866706.1"
                     /translation="MAMWSRLMTVEALVVVGVIGTLLYTYWFIRQKSLLKNLEQITEI
                     KHALIELRRVGWSERAIKKVFLKQLLPLKQEDIPAFVQNFIKETTIFASTSFYQLIRV
                     DSRSLSQEKATALLEQSMNMLDFPEELSHGILPELLQKMDVNCPPHHPFWRYFAKLVD
                     KVFPVRELEVKRPLNRQVHQLRYFISYQQAFLVRQQFGKEKTDWQALIAYLRSLPRWF
                     YRLRESARLHNKQLFGKKNQKTLPVNMKILIHFHSEFILNQDGQFALILEERPHVNGV
                     VNGASFNYARANNKRHRQLDMAPVGRQDPVFRKELLRSKMGVYLSPTRFRRYQKGRNE
                     VGWEQSYFNQQGSFSYGGHSRAACVANLRRRFAKDIGLKCTK"
     gene            complement(11885..14098)
                     /gene="recJ"
                     /locus_tag="U1099_000357"
     CDS             complement(11885..14098)
                     /gene="recJ"
                     /locus_tag="U1099_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEM4866707.1"
                     /translation="MIKPNYDWQLLTGFSDEQFIKIAKKEGVDPVAAKLLYERGIHSA
                     EELHTFLQPSLEDLHDPYLLHDMDKAVERIRRAIEDYEQILIYGDYDADGMTSASILK
                     ETLEEMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQGISLIVTVDNGVAGHEAIAYAQ
                     EMGVDVVVTDHHSMQETLPNAYAIVHPEHPEGNYPFKHLAGCGVAFKLACALLETVHA
                     DLLDLVAIGTIADMVSLTDENRVMVKYGLSLLKQTERAGLQELIKIAGIDIDSIDEET
                     VGFQLAPRLNALGRLDDPNPAIELLTGFDDEEAHQIALMIDSKNVERKDVVQAIYDEA
                     KTMLRQDRPVQVLAKEGWNPGVLGIVAGRLLEELQQPVIVLSIEDGKAKGSARSVEAV
                     DIFKALKDHQDLFIAFGGHAGAAGMTLEVDKLEELALTLTDYIIENKLDLSSKSSLVL
                     DEELDLEELTLDTLKSFEKLAPYGRDNKKPVFYIRDFQVESARTMGQNNAHLKLRISK
                     GAAGFDVVAFGKGNLALEFSQAKGLELAVTLSVNQWNGNTSLQLMLVDARVNGVQLYN
                     IRGKQHPIPAGVPILDIENPVADNKAIVLANLPEDLSSLRSYFQEREFEAIYFKNEIA
                     KPYYLDGYGSREQFAKLYKTIYQFDEFDVRYKLKDLAVYLKIKDSLLVKMIQIFQELE
                     FVTITDGVMRVNKDAQKREISESQIYQELKETVIQQELMALGTVQEIYEWLCGRV"
     gene            complement(14254..15801)
                     /gene="cls"
                     /locus_tag="U1099_000358"
     CDS             complement(14254..15801)
                     /gene="cls"
                     /locus_tag="U1099_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEM4866708.1"
                     /translation="MEKLWKIIYSRTFIVISLIILTIFLILWLVGSAATYMPALLIIL
                     QIFSIFVAISIINRPMNTSFKLTWIIFVIGIPIFGALFYFILQSNIETKRYRKNFHKQ
                     AQRLREYSKTSEKVMKALEQEDKEQLKLAHFMSEYVGYPIHTNTDAVYFSTGQEKFEA
                     LLEELKKAEQYIFMEYFIVDMGYMWDSILDILKEKAAQGVEVRFMYDGMNSLTNLPYN
                     YYKTLRKYGIKAKVFSQIIPALSTVQNNRDHRKIAIIDGKVAFTGGINIADEYINKKV
                     RFGYWKDAAIMIRGEAVANFTLMFLQMWNYNEKTETDDLKYLKTHKDLETETVDAEGF
                     FLPYGENPFDGDEVAKRVCLDMIQSATDYIYIMTPYLVLDDEMIDNLTYAAKRGVEVR
                     ILLPHIYDKKSAYLAARTDFPTYLEAGIEIFEFQPGFVHSKVVLVDDKKATVGTVNMD
                     FRSFYLNFECGLYIYNHSAVLAAIKDDFTHSFKKSQQINLLTFHKTYSWYKRLGGALL
                     RIISPLL"
     gene            16016..19201
                     /locus_tag="U1099_000359"
     CDS             16016..19201
                     /locus_tag="U1099_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4866709.1"
                     /translation="MKLSYKKRLLNQVLLASTVLFATSLAQGTVFANTEEIPPTTSET
                     VTPLPEETPITKTSTSEATDNLVEGKETEKQTEEIADTSPALVSTEEETTSSEPNAEE
                     TTLRSANNDNQDTTEEKSAVPTIDTVTLETKTVYLSEAVTITESVTLPDTTEKIEWTL
                     DGKPISEWKTWNLKEGDFTGDTFITVEESRQDNQLHLNIQLAALFGEDLSKRTPSNIR
                     RTYRHFIKDMLLEGTSADGNLIISKTLHFRPYEAYRTHEEMLTEIEETKNNAATDRLV
                     RIESIGQSAEGRDIKMAVVAKDQASIDKYLTETTPLMLTQPDQMLKQLQAGTFDYKLP
                     ILINNTHADEQPGIDVVTSLFKEFAQKDTITFPSTDADGNPVTLHLKVTDLLDKFIFL
                     FNFTENPDGDVKNLRSLVNGLDPNRDTGFQVNPETQAIVRQIHKWNPISVLDIHGFVS
                     GFLIEPATPPHDPNFEYDLLADIMLEKAHEMGRAGIANSKYERYTIPKVHWGDGWDDS
                     FSGYTAVYAMYHGILGHTIEIPEGNQESFKAGFFAVLGGVHNMATKPDSLMEMRLKYY
                     SRGVNKVEDPKAESELVGPDGAVVGRVKKDQPKFFPDYYVIPMTLDKHNDMQEAFKMI
                     EYFNRNGVVVKELTEDVGNFRKGDLVVDMAQAKRGFANHVLYAGSDESAWGAMYAELV
                     VNFPDMKGFRAKAVFEENAFSSKLGSITWTKAPRTTEIDFKAPYYVVTNTSESAVQAI
                     NQAIKSGAKVYLTDDGYILETNQFSHLLDTYALYGEPLYKKPIGQELKAMKVYAPSHS
                     YSWAGDFAILANAALAVERMGFEIVNSADEADAIILESDQFDASVFGKKPIIIVGGVA
                     MQKLEELGILAGFNAEQFTDGGDYEGLMRAIIDDKDPLTSGYAMNGLFYSNSGNWIEG
                     IPEGFKTLVKIADKDYYIAGWWPGHDKLANKIVAIAGNYQDQPVFIYAGNPTNKVHPV
                     HFFRWVSNALFGSQLATLEDLPAVEILVPQPEMPKNILDEAPKNTTVVHTTKSTEAKQ
                     LPQTGEKTNYIAIALGSLLLGSIALRRKERS"
     gene            complement(19239..19994)
                     /locus_tag="U1099_000360"
     CDS             complement(19239..19994)
                     /locus_tag="U1099_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM4866710.1"
                     /translation="MRTILITGASGGLVQEMVPLLKDDFLILLGRDVAKIEQLYVHHE
                     KKAVFDVDIRDEMALTAFFDELDSQFGQIDILVNNAGYAIYDDFENFSSQQVQDMFDI
                     NTFALMTMCRLVGKRMKVRRSGQIINIISMSGLIASAKSSVYSATKFAAMGFSNTIRL
                     ELAQYGVTVTTVNPGPIATGFFGQADPDGSYQESVKAFLLQPDYVAKKIVSAMGTKKR
                     DINLPWSLAAAHKLYTLFPRIADYLASTVFNLK"
     gene            complement(19991..20920)
                     /locus_tag="U1099_000361"
     CDS             complement(19991..20920)
                     /locus_tag="U1099_000361"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEM4866711.1"
                     /translation="MQIQFLGTGAGQPSKARNVSSLALKLLDEINQVWLFDCGEGTQN
                     QILETTIRPRKVAKIFITHLHGDHIFGLPGFLSSRSFQSSDEQTDIDIYGPVGIRSFV
                     LASLKVSGTRLPYRIHFHEFDVDTVGQVLETDKFTVFAEKLDHTIPCVGYRVIQKDLE
                     GTLDAEALRAAGVPFGPLFGKIKNGQNVTLEDGTEIIASDYISPPRPGKVVTILGDTR
                     KCHASVRLAVNADVLVHEATYGKGDEKIARKHGHSTNMEAAQVAKDAGVKQLLLNHIS
                     PRFLSKDISQLRKDASTIFEQVHIVKDLEEIEL"
     gene            complement(20931..21560)
                     /locus_tag="U1099_000362"
     CDS             complement(20931..21560)
                     /locus_tag="U1099_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEM4866712.1"
                     /translation="MTDYLDLAMKYGGFTSLDKVYLANKLAGLTDQQKLDFITPPPSV
                     INAYFAEIYQKQGAEEATDYYLTLSQQLCLFPKEPSFAESKPFVRLNLSGKSFGFTYV
                     SADGLAQVFSEGVEEVTESLLFEIAQVFPHYVVFVEEGKIFMKVNPFEDAELEDVETD
                     YLLTQVATTEGLVKISGFNQEEVLEVADGYAGQYYYVWSGRSAILYIKH"
     gene            complement(21544..22793)
                     /gene="hflX"
                     /locus_tag="U1099_000363"
                     /pseudo
     CDS             complement(21544..22793)
                     /gene="hflX"
                     /locus_tag="U1099_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
     gene            complement(22786..23670)
                     /gene="miaA"
                     /locus_tag="U1099_000364"
     CDS             complement(22786..23670)
                     /gene="miaA"
                     /locus_tag="U1099_000364"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEM4866713.1"
                     /translation="MKTKVIVVIGPTAVGKTALGIDLAQRYNGEIISGDSQQVYRKLD
                     IGTAKASPEEQAAAVHHLIDVRDVTEGYSAYEFVAEAKALIADITSRGKLPIIVGGTG
                     LYIQSLLEGYHLGGQVDQVQVLAYRAELDSLSDEDLETMAEQAGLMVEGNSRRRIIRG
                     LELKKFGENLENTESGYEPLYICLTDDRQVLYDRINQRVDKMMAAGLLDEVSWLYQEH
                     PQAQAAMGIGYKEFFPYLAGAISLEEAIDKVKQNSRRFAKRQLTWFRNRMQVPFYSVG
                     EPDCKSQIFTAVEEFLND"
     gene            23739..23909
                     /locus_tag="U1099_000365"
     CDS             23739..23909
                     /locus_tag="U1099_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEM4866714.1"
                     /translation="MAKGFGKGFLTGVLSTVALAAGAVFTVHKTIIEPEEKKEAFIEE
                     NRKKAARRRVAH"
     gene            complement(24008..25174)
                     /locus_tag="U1099_000366"
     CDS             complement(24008..25174)
                     /locus_tag="U1099_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM4866715.1"
                     /translation="MKKQSPFIIAGIVMLGVVMRAPFTALPAILIDVAAGLRVEVSSL
                     GILTSIPLIMFALCSSLAPRLAAKFGMEKLMALVLLVMVLGSGMRVLNLPALYIGTML
                     VGATIAFINVLLPSLVAANFPKKIGLYTTIYITLMGVAATVASMIAVPIVSSSSWEFF
                     ILLITGLVFMAFLIWLPNVRNNHRFASENQGNQKSSIWKNKAAIAFLIFGGLQSVLYY
                     TEITWLPTISQSVGFSKAEAGLMAGFFNMTAIPMSMIIPAVLSRQTKEMRRNIMLAIS
                     SVTLLGLVMMVLIPTNLILWSALHIILSFSNAALFPYMMLSFTLKTSNSQATAQLSGM
                     VQTGGYLIAAFGPGLLGYSYPIFGNWMPLILALAIVTLAMMWTIVLIEKEDIIL"
     gene            25377..26525
                     /locus_tag="U1099_000367"
     CDS             25377..26525
                     /locus_tag="U1099_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEM4866716.1"
                     /translation="MTTSRFQLVGSLLRPANLGEFKRQIEAREDIKYPFYDDFDGYKE
                     TEASLIQKIVAEQKENGLDIVTDGEFGRSMWHLDFLWGFDGIERYIAEHGYPFKDHDG
                     QIFETRKDIGLRITAPLSAKNHHFIDIFKQVKELAGETVTKQTIFGPAHAFNELTIFN
                     GQVGPNQVYKTRDELKVGLIAAYKEFLDQYKEAGGQIVQFDDCLWELFDPANPVPFLP
                     QDDSEALAALADEFVAINNAVIDYGHEIGLTVWTHNCRGNYESRSAAGGTYEAIAEKF
                     LRDQKYDRFFLEWDDERAGDLKALESLRDKNVEVVLGLLSSKTSDLDDEERVYKLLEE
                     ASKIIPKERLYLSHQCGFASCDSGNELAIPQQWAKIKQGQEIAEKFWS"
BASE COUNT         7873 a         6111 c         5168 g         7764 t
ORIGIN      
        1 ttgggcatcg ttacgatttt caattgtgat ggtacctgtt ggctgtgtaa cattgaccga
       61 actggaagct gtactgactc gagcacttgg aacacttgca gagccattcg aacttcctgc
      121 aatcgtattt gaagaacttc ttgtcagcga attcgaagta ctacttgttg cacctgctgt
      181 caaactagaa ttaaccgttg agcttgtagt agtcgaagtt gcttcctcct caattttaga
      241 cggagttgca tcactattat ccaaagcagt aggcttctca gccgcgctta cgacatcctt
      301 cttccactcg aaactataat ctgataaggt ctgaaaactt ccatctgtta attgtgctgt
      361 taacttcagc gaaaagactt ggtcttcctc cgcaaatgca gaccgatcca aaaccgctac
      421 gtatctaccc tgctcatcca ttgagaatgt gacagtaatg ccgctatttg tttcagtgct
      481 aaactgggct gtaacaccaa taatttcccc ttgaatgttc gatagaatag ctttaatctg
      541 accagcctca tcaacgacag ataactgccc gccagtttca gcaacctgag atgaagaaac
      601 ttgactagat ggggcattta ccatatccgc ttgaacaagc tgagcattca acgcggatgc
      661 acacaaagaa agaaataata acgttctttt tttcacaaac atctccttta tgtatttcac
      721 atgtaaaaac aactatattt ctcaaaatag atgcaattac actaatcttc tatttttctt
      781 cttgcttctt tgtcaataac caaccagcaa aaattgttat gagtcctagt acagttaatg
      841 gaagactaga tttccctgaa gtttttggca ataccttagc attatcattt gggataacct
      901 ttttctgacc tgcttcggaa atattgcctt tgcctgtagt tacatcagaa atctgaccgc
      961 ccccttgacc tggaacatcc tgctcatttg agccatttgt agcgctttga cttgtttgta
     1021 ccccggtatt ttcagtctta gctgtacttt tatcctcgac ttttgattgt agactagcag
     1081 ttagttctgc aatctgtcgg tccatttctg aaaagtcata cggaaatttt ggttgcgaag
     1141 cagttgcaag agcttgacca tatcctatag ttgcagatgt aatagattct aaattagagt
     1201 ttccactttc caataatcca gctaattctt gtccagttgc ttctgcataa gcaatataat
     1261 tatcaatcgc tgccgactct ttattcagta gttcaacgat aacttctcta gcgtatttca
     1321 atgcagccaa ttcggcttca ggagtacttg catcatcttc agactcgtac ttagaaacta
     1381 tagccgccgc ttcttctctt ccgctttttc cagcgactgt aagtgtctca aataagtaat
     1441 ggtaatgttg ggctatgcta ccaattaaat ttgcttcgag ttcatctaaa acaacaactt
     1501 gctctgctgt taagtttgcc ttcaagtctg caatttcttc atcccacgca gcttcagcac
     1561 tacccgaagc catttcttcc atcattccct caagaaacgc caatctatat tcttgcatat
     1621 cgatagtcat tttagcttga atatattcac gtacggcaac tttttcatca tcagttaaat
     1681 gataggtcgt actttgactt gtctctgctg ctacaactgg aacagctccg gacggtagtg
     1741 tagcacttcc tagtaaagcc agaaaaattc ctgacatcaa caacggctta acaatcgcct
     1801 tctttttcat acacatctcc tgtagactat ttcttttctt gtttatagaa tgcttccaat
     1861 gcttcttgcc aagttgggat gacaaaacct gtcgcctttg ccttgtccaa gctcatggtt
     1921 gagttgaatg gacgttttgc cttggctggg aactggctag aatccactgg caataccttg
     1981 gtgttggtat ccttgaggat ttccgtcgca aagtcaaacc aagtcgtatc ttctgctgca
     2041 tcgtttgaca aatggtagta accaaatgct tgcttggtgt ccaccaagtg taccatgaat
     2101 tccgcaaggg tacgggtcca agttggacga ccgtgttggt cattgacaac cgtcaaagta
     2161 tcgcgggttt ccgcaagatt ttgcatagtg aaaacaaagt ttttaccgta gttaccaaag
     2221 acccaagctg tacgaacagt gtagaatttg tcagcaaatt tctcaactgc ttcctcaccc
     2281 ttgcgcttgg tgcgaccgta ttcagattga ggatccggtt ggtcatctac ttgccactct
     2341 ttgccgactg gcaaatcacc attgaagaca tagtccgttg agatgtaaac gagggtcgca
     2401 cagtatttag cggctgcctt ggcaacattt tcagagcctg ttacgttaat cttgtagttg
     2461 agttccttgc cttcatcctc tgccatatca acagctgtgt aggctgcaca gtggtataca
     2521 acgcttggct ttacttctgc aaagaaggca tctactttat ctgcatccgt gatgtccatt
     2581 tctgctacgt cagcggcaac gtaatcaaca ccacgctcat ccaagagata acgcaattct
     2641 gtacccaact gaccatttgc acctgtaatt aaaatcatat ttgctcctta tttcaagaaa
     2701 ggcttgggca agagccctag cctttaataa tcttatttaa taacttcctg tgtcttagca
     2761 tagttggctt caacagcttc tttttcagct ttccaccagt cttggttatc tgtataccac
     2821 tggatagtct cttccaaacc ttgagagaag tctgtgaatt gtggtgtcca accaagctcg
     2881 tcacgaagtt tgcttgcatc aatagcatag cgcaaatcgt gtcctgcacg gtcagtcacg
     2941 tggtcgtaag catcttttgg ttgacccatt ttttcaagaa tcaattcaag cacttctttg
     3001 ttgttcttct caccgtcagc accgattagg taagtttcac ccatacggcc ttttgtcaag
     3061 attgcccaaa caccagtcga gtggtcgttg gtatgaatcc agtcacggac gtttttacct
     3121 tcaccgtaaa gttttggctt aatacctgcc aagatattgg tgatttggcg tgggataaat
     3181 ttctcgatgt gttggtaagg accgtagttg tttgaacagt ttgagatagt tgctttgaca
     3241 ccaaatgaac gcacccatgc tttgacaatc aagtctgaag cagctttggt tgatgagtaa
     3301 ggtgatgacg ggttatagtt ggtttccgca gtaaatttct cgcctggacc ttcgccatga
     3361 cctggcaaat cttcacgcaa aggaaggtca ccgtatactt cgtcggtcga tacatggtgg
     3421 aaacggatgt cgtatttacg agccgcttca agcaaagtgt aagtaccgat aaagttggta
     3481 tggataaatg ggcttggatc gttgagagag ttgtcgttgt ggctttctgc cgcatagtga
     3541 acgatggcat ctgccttagc agccaatttg tcaaccaact cagcatctgc aatgtcgcca
     3601 acaaccagct caacacggtc accaagaata gcttccaagt tagccttgtt tcctgcataa
     3661 gtaagtttat ccaaaacagt tacgtgaacg tctggatgat tgttatatac atagtgtaca
     3721 aagtttgaac cgatgaaacc agctccacca gtcacgataa tatttttaaa ttgagacatt
     3781 tttttctccg aaaagttttt tcttttttgt gtgtcatctt aggttgtggg gtcgtaagca
     3841 accaccaagg tgtttcatta ctaaaccatg agcctaaggt cccatggttt cccccgctcc
     3901 ttagcaaagc aaaggagctt ctcactacgg cgatgacaca cagaactgct cgcttcgctc
     3961 gcttttttac ctcaagattt ccttcaagta agcaatctta gcaaagtctt tttgattgtt
     4021 attttctgca cggtaggaat cttttgaaga ggcttggtag attttcaggt attgttcaag
     4081 tgtcggtagg taatagcgaa cacctactgt ttctagttct tccaaatctt ctagctctac
     4141 accagcaaat tcaggcagtg aatgtaggcc tccgaattca acagataagc cgtctttatg
     4201 aaattcatgt tcatgacgat caaccaggac atagccaagt tgattcatca gaacaatcag
     4261 ctcatctgca cggtaaatcc gctcctcatc tggcgcttcc catccccgtg ggtcactggg
     4321 cacatgaata tcaagatccc ttgcctgcca atctcgtcct gtccgccttt ccagaccgac
     4381 agaacccatt aacaagggag tgattcctaa cttgtttaac tgctctgcta tttccaaaaa
     4441 ggcttgaaac attagaggtc ctcttttctc aaaggcttca catctttgag gagtgggtga
     4501 tttttatctg cttctgaaac ttctgcttct tctaggtttt cccactggat gtcaaggctc
     4561 gggtcagcgt agttgacgaa ggcatactta ggcttaagct ccaaagccca gtagtcgttg
     4621 accaagtagc tgtaagccac gaaatctgac aagacttgga aaccattggc tactccgcgc
     4681 ggaacgaaga taccttttga agcatcgata acggtttggt aggtattacc gaaggtttcg
     4741 ccttcacgta gatcgaccca agttcccaaa accttaccgc cgtctgctac tgaaatgtat
     4801 ttatcccaag gctctgcgtg aagaccacga agaacgtttt tacgagagaa ggaaacatta
     4861 ttctgcaatt ttccttctgc aaagaagctt tcaggaaagc cgagaggcaa cattttttcc
     4921 ttttggaagt tctccttgaa ccagccacgg ttgtcgccat gaacaggaat gtcgaattct
     4981 aacattcctg gaatagcttc aactgggcga gctgctagtg ttttaccgaa aaagttttct
     5041 gtcattaagc ttctccaatc aaacggagca agtattgtcc gtattcattt ttcttaagcg
     5101 gttgtgccaa ttcatacact tgttcttttg taatgtagcc catgcggtaa gcaatttctt
     5161 ccaagttagc cacttgaacg ttttgcaaac gttgaaccgt ttcgatgtac tgtgcagctt
     5221 ccaagaggct ttcatgcgta cctgtatcca accaagcaaa tccacggccc atcaattcaa
     5281 ctgataaatc acctcgatct aagtaagcct tgttgacatc tgtgatttcc aactcgccac
     5341 gaggtgaagg cttgatattt ttcgcaatct ccacaacatc attgtcatag aaataaagac
     5401 cagtaacagc aaaattcgat tttggctctt caggtttttc ttcgatagag atggcattca
     5461 tatcagcgtc aaactcaaca acaccaaacc gttctggatc ttttacttga taaccaaaga
     5521 cagtcgctcc cttttctttg ctagcagcac gttgcaacat cttggtcaaa ccatttccgt
     5581 ggtagatgtt atcaccaaga atcaaggcta cattgtcatc accgataaat tcttcaccga
     5641 taataaaggc ttgcgccaaa ccatctggac ttggttgttc tgcatatgaa agtgaaattc
     5701 caagttcaga accatctcct aacatatcct taaaacgtgg aagatcttgc ggagttgaaa
     5761 taatcaaaat atctttgatt ccagccaaca tcaatgttga caatggataa tagatcattg
     5821 gtttatcgta aattggcatc aactgttttg aagctgcgcg cgtcaaagga tacaagcgtg
     5881 tacctgaccc accagctaga ataatacctt tcatataaga gtaccttccc taaattaata
     5941 tcagttcatt ataccatatt tttaaagata ttgtcataag aaaaaagcct gtgacgacag
     6001 actttaacaa attatcctag agcaacatcc aaaatcatca tgatgataaa accagccatt
     6061 aagcccagag ttgcaatatc cgtattgccg tttgtctgcg attctggaat aagctcctct
     6121 acgacgacaa aaatcatggc tcctgccgca aaggagaggg cataaggtaa aataggtgtc
     6181 ataaaggtga ccgcaaaagc cccgatgact gctgcaatcg gctcgacaat tgctgacatt
     6241 gatccccagt aaaaagcttt ccacctagat gcgccgtctg tacgaattgg aatagctaag
     6301 gctgcacctt caggaatatt ttgtataccg atcccaatag caagaccgat tgcgccgata
     6361 aaagctgctg gactatagtt agaagctagt gcaccaaatg tcacaccgac cgccaagcct
     6421 tcaggaatat tgtgaattgt aatagctaag aagagcaggg ctgttttgga tagatttttt
     6481 ctatccttct caccaccacc ttctgcctta tctttatcat ttcccaaatg caaatgcgga
     6541 acccaggcat ctaccaagcg taaaaaaata ccacctgctg caaaacctac tgctgctggt
     6601 atccaagcca gattaccgta tgaactttca gcatactcaa tcgatggggc taataaagac
     6661 caaaaagaag ccgcaatcat aacaccagct gcaaagccta gcatggtatc tagcaaacga
     6721 cgactaactg tcttaaagaa aaatacaacg gcagacccca taatcgtaca aaaccatgtg
     6781 aataaaccac ctagcaaagc ctgtagaact acagactgat tagtaaccca agtcataact
     6841 aacatcctat cctgtttaat cttgcatatc ctataaagta agatacccta atttattata
     6901 aaattcaagc tttttcactt aaataaataa gaaaatatct aaaaaataaa agacctaaaa
     6961 ctacattaaa tgtcgtcttg gtctttccac aatcaagtgc tgattaattt atcacaaatg
     7021 tctaaacgga ttggttgaaa cagttgaagg tagaatttcg acatcccaac cttcttttgc
     7081 cttccatatt tccaacaatt cagcaatttt cttgataaat aatgcttcga tatgatggcc
     7141 tggatcaata gccagcaagc cctgagtcag catgtcctgc ccagtatggt agtaaatatc
     7201 gcccgtgatg taaacatctg cccccttagc aagagcctca tggtagtaag acccaccact
     7261 gcctccacag atggctacac ggctgacata ctgcttggct tggtgcccat aggtcactag
     7321 acggacagca tctagaccaa agacagactt gactttcaaa gccaattctt ccaaagtctg
     7381 ctctgctatt gtccccacac ggccaagccc ctgcccctcg gcagtttcaa tcaagtaggt
     7441 agtatcttga atttctaata gttcacagaa ccagtcgttg agaccaccct caaccacatc
     7501 aatatctgta tgactaacat agactgcaat atcatgctta atcaaatcaa gatacatttt
     7561 agtttgcgga ttgtcagcca ccaaatcatt tattggacga aaaatcggag cgtgcttgac
     7621 tataatcaaa tctacctttt tatcaatcgc ctctgccact gttgtctcac gaatatccag
     7681 agccaccatg acacgttgaa tctctttatt aagggtaccg atttgcagcc ctttgacatc
     7741 gccttccata gccaaagaag ggggacaaaa ggcttgatag cgctcaataa cctgactagc
     7801 taacatggac tacctcctga atttgttgga tcttttggga aatggcaaag cggtcacctt
     7861 ggcgttcaga tggaacttgt tccaaagcaa aacttaattt ttcaagttct cttctccact
     7921 ttttgacgaa aactggtgag gcctcctcta gtagataggg accaaaacga tgttcaactt
     7981 ctgacaatct catctgtcct gattctgcaa cgaggacttc atataacttg ccattttctt
     8041 ccagaatctt ttcagcaacc aatctaaaac catttttttc caaccatcga cgcacatcat
     8101 cttcacgatt atttggctgg agtatcaaac gttcaatggt cgcaagcttt tcttttcctg
     8161 cttctaagat ttccgcaatc aagcgtcccc ccatacctgc tatggtcacg gcagtcatct
     8221 ggtcagactt ctcaaacgcg cctaagccat ttgccaaacg gactagaatt ttctctgttt
     8281 gtcccgcctg ctctacattc tggagcgctg actgataggg accttgaacg acttcccccg
     8341 caatggcaaa atcaatacgc ccctgctcta ccaaaaaaag agggagataa gcatggtcac
     8401 tccctacatc aactagacgt gccccttgtg ggacaaaact tgcgactgtt tccagccttt
     8461 tagataattt tgtttccata tcaatctttc cagagatcca ttgcatcaag aaagtcactt
     8521 gaaacagtca cattcttagg agtctggatt tctctttctg catttttcgc ttctacttgc
     8581 gcaactgtag taattccctc acggcgccag tttcgtagaa tagcttggat gtatttccaa
     8641 tttgttttgt tgttaaaaac agcttctttc aaggctgcac gaaccaaatc aatcgaagtt
     8701 ttgtcatcct caatagtctt ctgcaaatct tcaatttcaa acggcgataa aaagcggccc
     8761 aattcacgtt caaaatcacc caccaaggtc ttcaaatcat tctctggttg cacaatctca
     8821 actgcctgtt ttggagttaa cagaacatcc aatttttcaa aagcaggaag cgcatcgaat
     8881 atcatttcaa tttcaccagc aatactgatt gtcttaaatt ctaagagtcc agcatcttgg
     8941 agattttcaa tagcttgatt gacctgagca actgttttac cagtagcttg agcaatctca
     9001 ctaggcgcta atgattcgat agctgaagaa ttttgatata agaaaaactg ccaaatcaaa
     9061 aaatcatctg ccgacggaaa aagctcttga tagtggaata atattgctgc tggcaggacc
     9121 aaatgtccct gacgaaattg ctgtgaatag ttcatacttc ctctttaaag actagctgac
     9181 tgggctgatt gttcaaagat atgccagtcg tatggagttt cttgatagac agcataattc
     9241 acccagttgg taaagaattg tgctgctggt aaattccaag agagagatgg tttgcttgta
     9301 gggtcatctt ctttgaaata attttccggt aaatggggat tttttccagc tatacaatca
     9361 cgttgatatt ccttagctag tgtatcccta tcatactcta aatgtccaaa actataaatt
     9421 tctcttaaat ccttgctggc tagaatggat agaccaactt ctggaccttg tgataaaatc
     9481 gtcaacccac tcagatgggc aatatctgct tctcttactt ccgtataacg ggaatgtggt
     9541 gaacgaaatt catcgtcaaa tccacgcata agagggctcg ttggattagt cacttcttga
     9601 caatataccc ctgatagttt tcgagacata ctgtgtttgg gtacaccata gcgtgcatgc
     9661 aaacctgcct gtgcacccca acaaatgtgc aatgttgaat aaacatgtgt ttttgaccaa
     9721 tcaaaaactt gagtaagttc ttgccagtaa tcaacttcct caaaaggtaa attttcaact
     9781 ggtgctcctg tcacgatgag cccatcaaaa tagctatccc tgacctgctc aaaagtctta
     9841 taaaactgct tcaaatggtc cgagtgtgta tttttggact cgtgactagc catgtagaga
     9901 aattcaacct ctagctgtaa aggagtgtta gccaaaagac ggagcaactg agtctccgtc
     9961 actatcttct gtggcatgag attgagaata aggattttga taggacgaat gtcctgatga
    10021 ctggctcggt cactatccat cacaaaaata ttctcttttc tcaaaatttc aactgctggt
    10081 aatgattttt caattttaat tggcataagc aacctcccaa ttttcttata tttattataa
    10141 gacaggttgc tatgcaattc aattagaata aaaataggac cagttataag ttttggctat
    10201 aaccttagat taatagtgca tcaaaacctt gtagtcgtaa tcaccgattg cttcgcggcc
    10261 tttcaaatca tctagctcaa tcaagaaggc acagccagca acgataccgc caagcttttc
    10321 aatcatttcg atcgttgctt ttacagtacc accagttgcc aacaagtcat caacgataag
    10381 aacacgttga cctggcttaa tcgcatccgc atgcatcgtc aatgtatcta caccatattc
    10441 tttttcatag tcagctgaga taacctcacg tggtaactta cctggcttac gaactggtgc
    10501 aaaaccgatt cccaattcaa aggcaactgg acatccaaca atgaaaccac gcgcctctgg
    10561 cccaacaatc atatcgattt gcttatctgt cgcatactga acgatttcac gtactgcata
    10621 gctataagca tttccatctg ccatcaaagg gctaatgtca cggaattcga taccttcttt
    10681 tggatagtta ggaattgttg cgatgtaatc ttttaagttc atttgtctct ctctttttaa
    10741 aaataatttt tactcttcat tataccatga aattgttatt tggtacattt cagacctata
    10801 tctttagcaa atcgtctcct caaattcgcc acacaagctg ccctgctatg cccaccatag
    10861 gaaaagcttc cttgctgatt aaagtaagac tgctcccagc caacttcatt tcttcctttt
    10921 tggtagcgac gaaagcgagt aggcgataaa tagactccca tctttgagcg tagcaattct
    10981 ttccgaaaaa cgggatcctg cctgccaact ggagccatat caagttgtct atgacgctta
    11041 ttattagctc tggcataatt aaaggaagca ccgtttacca cgccattgac atgaggtcgt
    11101 tcttctaaaa tcaaggcaaa ttgtccatct tgatttagga taaattcact gtgaaaatga
    11161 atcaaaatct tcatattgac tggcaaagtc ttctgatttt tcttgccgaa cagttgttta
    11221 ttgtgtaggc gggcagattc tcgtaagcgg taaaaccatc gaggtaggct acgtaaatag
    11281 gctatcagag cctgccaatc tgttttttct tttccaaact gctgacgtac caaaaaagct
    11341 tgctgatagg aaataaagta gcgcagttgg tggacctgtc tgtttaacgg ccgtttgact
    11401 tctaattccc gcactggaaa aaccttatca actagcttag caaagtaccg ccaaaacgga
    11461 tggtgtggag gacaattcac atccatcttc tgcaaaagtt caggtaatat cccgtgactt
    11521 aattcctcag gaaaatctaa catattcata ctttgctcca ataaagccgt cgctttctct
    11581 tggcttaaag agcgactgtc aactcttatc aactgataaa agctggtcga ggcaaaaata
    11641 gtagtttctt ttatgaaatt ttgaacaaat gctgggatgt cttcttgttt tagaggcaac
    11701 agttgtttca ggaaaacctt tttgatggct ctttctgacc atcctactcg ccgtaactca
    11761 atcagtgcgt gctttatttc ggttatctgt tcaagatttt tcaacagact tttttgcctg
    11821 ataaaccaat aagtgtagag aagcgttcca atcactccaa caacaaccag agcttctact
    11881 gtcatcaaac gcgaccacat agccattcat aaatttcctg aacggtgcca agcgccatca
    11941 actcttgttg gataactgtt tctttcaatt cttgatatat ttgactttct gaaatctctc
    12001 gcttctgtgc atctttgttg acccgcatga ccccatccgt aatcgtgaca aattctaatt
    12061 cttggaaaat ctgaatcatt ttaaccaata gactgtcctt aatttttaag taaacagcca
    12121 aatctttcag tttgtagcga acatcaaact catcaaattg gtaaatagtc ttatacaatt
    12181 tagcgaattg ttctcggcta ccataaccat ctagatagta cggtttggca atttcgtttt
    12241 taaaataaat ggcttcaaat tctctttcct gaaaatagct acgcagacta ctgaggtcct
    12301 caggcaagtt agccaagaca attgctttgt tatctgctac tggattttca atatccaaaa
    12361 ttgggactcc agcaggaata ggatgttgct ttccacgaat attataaagt tgaacaccat
    12421 tgacccgagc atcaaccaac atgagctgaa gactggtatt gccattccat tgattgacag
    12481 acaaggtgac agctagttct aagcccttgg cctgtgaaaa ttccaaggct agattgccct
    12541 taccaaaagc aaccacatca aaacctgccg cacctttact aatgcgtagt ttcaaatggg
    12601 cattattctg ccccatggtc cttgctgatt ccacctgaaa gtctctgatg tagaatacag
    12661 gctttttatt atccctgccg taaggcgcaa gcttctcgaa agattttaga gtatctaggg
    12721 ttaattcctc taaatccaat tcttcatcta agaccagcga agacttgctg gataaatcca
    12781 atttattttc gataatgtag tctgtcaagg tcagagcaag ttcttcaagt ttgtcaactt
    12841 ccaaggtcat acctgctgca ccagcatgac caccaaaggc gataaatagg tcctgatgat
    12901 ctttaagagc cttgaaaatg tcaactgctt caaccgaacg agccgacccc tttgccttac
    12961 catcttcaat agacaagacg ataacaggtt gctgcaattc ttctagtagg cgtcctgcta
    13021 caatccctaa aacacctggg ttccaacctt ctttagccag aacttgaacc ggtctatcct
    13081 gacggagcat ggtttttgct tcatcgtaaa ttgcttggac gacgtctttg cgttctacat
    13141 ttttactgtc aatcatgaga gcaatttgat gggcctcttc gtcatcaaaa ccagtcagta
    13201 attcgatggc tggattagga tcgtcgagtc gaccaagggc attcaacctc ggagcaagtt
    13261 ggaaaccaac tgtttcttca tcaatactgt ctatgtcaat accagctatc tttatcaact
    13321 cttgcaaacc tgctcgctca gtctgtttga gaagcgagag accatatttg accatgacac
    13381 gattctcatc ggtcaagcta accatatcag cgatggtgcc aattgcgacc aaatccagca
    13441 agtctgcatg aacagtttca agaagagcac aggccaattt aaaagccaca ccgcagcccg
    13501 ccaaatgttt gaagggataa tttccctctg gatgctctgg atgtacaatc gcataggcat
    13561 tgggaagggt ttcctgcatg gaatggtggt ctgtcacgac aacatcgaca cccatttcct
    13621 gggcataggc aatcgcttca tgacccgcta cgccattgtc caccgtcaca ataagagaaa
    13681 tcccctgttg ctcgataaaa tacttgtaaa ctgactgatt cggcccatag ccatctgtga
    13741 aacggttggg aagataaact tggacttctg cccccatttc ctccaaagtt tctttcagaa
    13801 tagatgccga ggtcatgccg tctgcgtcat aatctccata aatcaaaatt tgttcgtaat
    13861 cttcgattgc tcggcgaatc cgctctaccg ccttgtccat atcatgtagt aaatagggat
    13921 cgtgaagatc ttctaagctt ggttgtaaga aagtatgcaa ttcttccgcc gaatggatgc
    13981 cacgttcata cagcaactta gccgctaccg ggtccactcc ttctttttta gcaattttga
    14041 taaattgttc atcagaaaaa ccggtcaata attgccagtc ataattgggt tttatcacgt
    14101 tttttactcc ttaatactca aaatcacaag tagcctgtaa attttccgag tataaactat
    14161 ctttctatta tatcattttc caagaaaaat ggagcaaggt aagaccgaaa cagaatgtcg
    14221 atcccttgct ccatgaagaa cgaatattgc aatttataat aacggcgaaa taattcgtag
    14281 tagggctcct cccaaacgct tataccaaga gtacgtttta tggaaggtta gaaggttaat
    14341 ttgctgtgat tttttgaagg aatgagtaaa gtcatcctta atagctgcca aaactgccga
    14401 gtgattgtaa atatagagac cgcattcaaa attaagataa aaagagcgaa aatccatatt
    14461 caccgtacca acagtagctt tcttatcatc gaccaaaaca actttggaat ggacaaatcc
    14521 aggctggaat tcaaagattt caattcctgc ttccaagtaa gtcggaaaat cagtgcgagc
    14581 agccaaatag gctgattttt tatcgtagat atgcggtagt agaatgcgaa cctcgactcc
    14641 acgcttcgct gcataagtca aattatcaat catttcatca tccaacacta ggtagggtgt
    14701 catgatataa atataatccg ttgccgactg aatcatgtct aggcagactc gtttggctac
    14761 ctcatctcca tcaaatggat tctcgccata cggaaggaag aaaccttcgg catcaacggt
    14821 ttccgtctct aaatctttat gcgttttgag gtattttagg tcatctgtct ctgtcttttc
    14881 attgtaattc cacatttgaa ggaacatcaa cgtaaagttt gcaacagctt ctcccctaat
    14941 cataatcgct gcgtctttcc agtaaccaaa acgcactttc ttgttgatgt attcatctgc
    15001 gatattaatg ccaccggtaa aggcaacctt tccatcaata atcgcaattt ttctatggtc
    15061 acggttattt tgcaccgtag ataaggctgg gataatttga gaaaatactt tggcttttat
    15121 cccatatttt cgcaaggttt tatagtaatt atagggaaga ttggtcagtg aattcatccc
    15181 atcatacata aaacgaactt ccaccccttg cgctgccttt tccttcaaaa tgtcaaggat
    15241 agaatcccac atatatccca tatcgacaat aaagtactcc atgaaaatat actgctctgc
    15301 ctttttcaat tcttctagta gagcttcaaa tttttcctga cctgttgaaa aatagacggc
    15361 atccgtattg gtatgaattg ggtagccaac gtactcactc atgaagtgag ctaatttcag
    15421 ttgctcctta tcttcctgct ccaatgcctt catgaccttt tcagacgtct tactatactc
    15481 ccgaagtctt tgtgcttgct tatgaaaatt tttacgataa cgtttggttt caatgttgga
    15541 ctgcaaaata aaatagaata gagcaccaaa aatcggaata ccaatgacaa aaataatcca
    15601 ggttaactta aaactggtat tcatcggtct attgatgatt gaaatagcca caaaaatcga
    15661 aaaaatttgc aaaataatca ataaagcagg catataggta gctgccgaac ctaccaacca
    15721 aagaataagg aaaatggtta gaataatcaa actaatgaca ataaatgtcc gactatatat
    15781 aattttccaa agtttttcca tacatctctc ccttattttt taccattata acagatttta
    15841 gttcattatc ctactcaaaa taaatagtct ctatgattcg atatttctga atattttaaa
    15901 tatataaaca tattcaattt atattgtaaa gacatatatt ttttgataag ataagcatga
    15961 aataacccgt tttaatttat taccccgtac acattatcaa aaggaggaag ttcgcatgaa
    16021 actttcatat aagaaaaggc tgttgaatca agtcctactt gcctcaacag ttctttttgc
    16081 tacatctctc gctcagggaa cggtcttcgc aaatacagag gagataccac ctactactag
    16141 tgaaacagtg actccactgc ctgaagaaac tcctataacg aaaactagca caagcgaagc
    16201 tacagataat cttgtagaag ggaaggagac agaaaaacaa actgaagaaa tagcagatac
    16261 ttctcctgca cttgtttcca cagaagaaga gacaacatct tccgagccca atgccgagga
    16321 aactacgctc agatcagcta ataatgataa tcaggatact acagaagaaa aatctgccgt
    16381 accgactatt gatacagtta cactcgaaac aaaaactgtc tatctatcgg aagcagtcac
    16441 tatcacagaa tccgtcacac ttccagatac gactgaaaaa atagaatgga cattggatgg
    16501 aaagcctatt tccgaatgga aaacctggaa tttgaaagag ggagatttca caggcgatac
    16561 atttatcaca gtagaagaaa gcagacagga taaccaactt catttgaata tccagctcgc
    16621 agctctcttt ggagaagatt tgagtaaacg gacaccgagt aatattcgcc gtacctatcg
    16681 acattttata aaagatatgc tgttggaagg tactagtgcc gatggaaact tgataatttc
    16741 aaaaactctt catttccgtc cttatgaagc ctaccgtaca catgaggaaa tgttgacaga
    16801 gattgaggaa acgaaaaata atgctgcgac cgaccgccta gtaaggattg agtcaattgg
    16861 tcaatcagct gaaggtagag acattaaaat ggctgttgta gctaaagatc aagcaagtat
    16921 tgataaatat cttactgaaa caacaccact catgctgacc caacccgacc agatgctgaa
    16981 acagttgcaa gctggaacct tcgactataa attacctatt ctcatcaaca acactcatgc
    17041 tgatgaacaa cccggcatag atgtggtaac tagcctattc aaagaatttg cacaaaaaga
    17101 taccatcacc ttcccatcta cagatgccga tggaaatcct gttactcttc atttaaaagt
    17161 gacagatttg ttagataaat ttatcttctt gttcaatttc acagaaaatc ctgatggcga
    17221 tgttaaaaac ttgcgttcac tagtaaatgg tttggaccct aaccgtgata ctggtttcca
    17281 agtgaatcca gaaacacaag ccatcgtccg tcaaatccac aaatggaatc caatatctgt
    17341 tttagatatt catggttttg tatctggatt tctcatcgag cctgctactc cgccacacga
    17401 cccgaacttt gaatatgatt tactagccga tattatgctg gaaaaagctc atgaaatggg
    17461 ccgagcaggt attgcaaatt ctaaatatga acgctacacc atccctaaag tccactgggg
    17521 agatggttgg gacgattcct tctcaggcta tacagcggtg tatgcaatgt accacggtat
    17581 tcttggacac acgattgaaa ttccagaagg aaatcaagaa tcattcaagg cgggattctt
    17641 tgctgttttg ggcggtgttc acaatatggc aaccaagcca gatagtctta tggaaatgcg
    17701 tctcaaatac tattcacgcg gtgtcaataa ggtagaagat ccaaaagcag agtctgaact
    17761 agtcggacca gacggagctg ttgtcggacg tgtcaaaaaa gaccagccaa aattcttccc
    17821 agactactat gtgattccta tgacgctcga taaacacaac gatatgcaag aagcatttaa
    17881 gatgattgaa tatttcaatc gtaatggggt tgttgtcaag gaattaactg aagatgttgg
    17941 aaacttccgc aagggcgacc tagttgtcga tatggctcaa gccaaacgag gattcgctaa
    18001 ccatgtcctc tacgctggtt ctgatgaatc tgcctgggga gccatgtatg ctgaattggt
    18061 ggtcaacttc ccagatatga aaggctttag agccaaggct gtatttgaag aaaatgcctt
    18121 tagcagcaaa ttaggtagca tcacatggac aaaagctcca cgtactacgg aaattgattt
    18181 caaagcgcct tattatgtcg tcacaaacac ctctgaaagc gctgtccagg ccattaatca
    18241 agccatcaaa tcaggcgcta aggtctacct gacagacgat ggctacatct tggaaactaa
    18301 ccaatttagt cacttactgg acacctatgc tctatacggc gagcctctct ataagaagcc
    18361 tattggacaa gaattgaaag caatgaaggt gtatgctcct tctcactctt atagttgggc
    18421 aggcgacttt gccattcttg caaatgctgc acttgctgta gaacgaatgg gctttgaaat
    18481 tgtcaatagc gcagatgagg ctgatgctat cattctcgaa tcagaccaat tcgatgccag
    18541 tgtatttggt aagaaaccaa ttatcatcgt aggtggtgtc gccatgcaaa aactggagga
    18601 attaggaatc ttagcaggtt tcaatgcgga acaatttaca gatggtggtg actatgaagg
    18661 tctcatgcgg gctattattg atgataaaga tccattaaca agtggctacg ccatgaatgg
    18721 acttttctat tccaactccg gtaattggat tgaaggtatt cctgaaggat ttaaaacgct
    18781 tgttaaaatc gctgataaag attattatat cgcaggttgg tggcctggtc atgacaaatt
    18841 agccaataaa attgtcgcca tcgctggaaa ttatcaagac caacctgtct ttatctatgc
    18901 aggtaatcca acaaacaagg tccaccctgt tcacttcttc cgttgggtgt ccaacgccct
    18961 atttggtagt caattagcaa ccttagaaga cttaccagct gtagaaattc ttgtaccaca
    19021 acctgaaatg ccaaaaaata ttttggatga agcaccaaaa aatactacag tagtacatac
    19081 caccaagtct accgaggcaa aacaactacc tcaaacaggt gaaaaaacaa actatatcgc
    19141 tatcgcatta ggtagcctac ttctaggcag tatcgcctta cgtagaaaag agcgtagcta
    19201 gtctacacga aaacgccgag ttttatttcg gcgtttttct atttcaaatt aaatactgta
    19261 ctggccaagt agtctgctat tctcggaaag agagtataga gtttatgagc agctgctagt
    19321 gaccaaggca aattgatgtc tcgttttttc gtccccatag ccgaaacaat cttcttggca
    19381 acatagtctg gttgcagcaa gaaagccttg acgctctctt gatagcttcc gtcaggatct
    19441 gcctgaccga aaaagccagt cgcaataggt cctgggttaa cagttgtaac tgtcacacca
    19501 tactgggcta attccaagcg aatggtattt gaaaaaccca tagctgcaaa cttggtcgcc
    19561 gaatagaccg aagacttggc agaagcgatc aagcctgaca tggaaatgat attgataatc
    19621 tgcccgctcc gtcttacctt cattcgttta cctactaaac ggcacatggt catcagagca
    19681 aaagtattga tgtcaaacat atcctgcacc tgctggctgg aaaaattctc aaaatcatca
    19741 taaatggcat agccagcatt attgactaaa atatcaatct gaccaaactg actatccagt
    19801 tcatcaaaaa atgccgtcag ggccatttca tctcggatgt ccacatcaaa gacagccttt
    19861 ttttcatgat gaacatagag ctgctcaatt tttgccacat cacgacctag taaaatcaaa
    19921 aaatcatctt tcagcaatgg caccatttcc tgtaccaagc caccagaggc acctgtgatt
    19981 agaatggttc tcatagctca atttcctcta aatccttgac aatatgaacc tgctcgaaaa
    20041 tagtacttgc atctttccgt aattgactaa tatccttgga taaaaatcgt ggactgatat
    20101 gattaagcaa gagctgcttg acaccagcat ccttggcaac ctgagcggct tccatattgg
    20161 tcgaatggcc atgcttgcga gcaatcttct catcgccttt tccgtaagtc gcctcatgca
    20221 ccagcacatc agcattgaca gccagacgga cactagcatg acacttgcgc gtatcaccaa
    20281 gaatggtcac taccttacca ggccgaggag gagaaatata gtcactcgcg ataatttcag
    20341 tcccatcttc tagcgtcacg ttttgaccat ttttgatttt accgaataag ggaccaaatg
    20401 gcacacctgc cgcgcgcaga gcctctgcat ccaatgtccc ttccaaatct ttctggatga
    20461 cacgatagcc gacacacgga atggtatggt ccaacttttc agcgaatacc gtgaatttgt
    20521 ccgtttctag gacctggccg accgtatcaa catcaaactc atgaaaatga atgcgataag
    20581 gtaggcgtgt ccctgatacc ttcaaacttg ccaagacaaa ggaacggatg ccaacaggac
    20641 cgtaaatatc aatatccgtc tgctcatcgc tagactggaa agaacggcta gacaaaaaac
    20701 caggcagtcc aaaaatatgg tctccgtgca ggtgagtgat aaagattttt gcgaccttac
    20761 gtggacgaat agtcgtttct aaaatttgat tttgagttcc ttcaccacaa tcaaagagcc
    20821 aaacctgatt gatttcatcc aagagtttca aggccaaact ggatacattt cgagccttgg
    20881 agggctgacc agcccccgta cctaaaaatt gaatttgcat tgtttacttt ctaatgtttt
    20941 atatataaaa ttgcagagcg acccgaccag acatagtaat actgaccagc atagccgtcc
    21001 gccacctcca gcacttcttc ctgattaaag cctgaaatct tgaccaaacc ttctgtcgtc
    21061 gccacttggg tcaaaaggta gtctgtctca acgtcctcta actcggcgtc ctcaaaggga
    21121 ttgactttca tgaaaatctt gccctcctcc acaaagacga catagtgggg aaagacttgg
    21181 gcgatttcaa agagcaaact ttccgtcact tcctctacac cttccgaaaa aacctgggcc
    21241 agaccgtccg ccgacacata ggtaaagccg aaagacttac ctgataaatt caggcggaca
    21301 aagggcttgc tttcagcaaa gctgggttct tttggaaaaa ggcagagctg ctgagaaaga
    21361 gtgagatagt agtccgtcgc ctcttctgcc ccttgtttct ggtagatttc cgcaaaatag
    21421 gcattgataa ccgaaggcgg tggcgtgata aaatctagtt tttgttggtc tgtcaaacca
    21481 gctaatttat tcgccagata gaccttgtcc agactggtaa agccaccgta tttcattgcc
    21541 aaatcaagat aatccgtcat aaaattcttc caacttccat ttattagcgg aagcgatata
    21601 gcctgatacc acttccacat cctcttcata ggtccgattt tctatcaggg caagagttgc
    21661 taaatcatgc aatttataag attcagcaag tggaaccctg acctcaaatc gttcaaacat
    21721 ggtcttaatc ttggccaaga ccatcatctg aatatgacct ttggcatttt catccttagc
    21781 ggacagcatg acatgtggga actgactggg ggtaaagctg tcatcggtct tgtccaactt
    21841 gttgtagaga gctagacgtg gaatgtccag catatccagg tcttttagaa tgtccaagac
    21901 cacctgctcc tgctcactgt gattgggtca gaggcgtcga tgatatggag caagagatcc
    21961 acgttcatgg actcctccaa ggtggacttg aaggcagaaa tcaactcagt cggcaggtcc
    22021 tgaatgaaac caaccgtatc ggtcaaggtc acattaaatt tgtctgccaa gttaatctgc
    22081 ttggttgtgg catctagtgt ggcaaaaagt tcatcagcct cgtactgtcg cttgtccgtc
    22141 atgacattca taatagtgga cttacccgcg ttggtatagc caatcaagcc gattttgaac
    22201 acgcctgatt gcaaacggcg ttcacggaca gtcgctcggt tcttctccac tgttttgagt
    22261 tggcgctcaa tgtcatgaat ctggttacga atgctccggc ggttaagctc caactggctt
    22321 tcacctggtc cacgagagcc gataccacct gcctgacggc tgagcatgat gccctgacca
    22381 accaagcgtg gcaacatgta cttgagctgg gctaggtgga cctgcaattt cccttcgtga
    22441 ctccgtgccc gcatggcaaa aatatccaag atcaactgca tgcgatcgat taccttgaca
    22501 cctagaattt cttccagatt gacattctgg cggggtgtta atcgattgtt gacgatgaca
    22561 gtatcaatct cgtcagcttc gaccatctgg gcaatttcgt ccagcttacc agaaccgaca
    22621 aaagtcttgc tgtcgtattt ttctcgtttt tgagtgtaga ctcccttgac cagagcacca
    22681 gccgtcttag caagacttgc caactcctcc atggacatgt caaagttgtc tgtctgctgt
    22741 aattccactc caaccaggag ggcgcgttct tgttctttct gcgtttcaat catttaaaaa
    22801 ttcctccacg gcggtgaaaa tctgcgactt gcaatctggc tcgcctactg aatagaaggg
    22861 aacctgcatg cgatttctga accaagtcaa ttggcgtttg gcaaagcggc ggctgttttg
    22921 cttgacctta tcaatagcct cttccagcga aatggctcct gcaagataag ggaaaaattc
    22981 tttgtagcca atccccatgg ctgcttgggc ttggggatgt tcttggtaga gccagctaac
    23041 ttcatccagt aagcccgccg ccatcatctt atccactcgc tgattgatgc ggtcgtaaag
    23101 aacctgtctg tcatccgtca aacagatgta aagtggctca tatcctgact ctgtattttc
    23161 taagttttcg ccaaattttt tcaattccag tccacgaatg attctccgtc ggctgtttcc
    23221 ctcgaccatc aaacctgcct gctctgccat tgtttccaaa tcctcgtcag acaagctatc
    23281 aagttctgcc cgataagcaa gaacttgcac ctggtcaact tgaccgccca gatggtagcc
    23341 ttctaacaga ctttgtatgt aaagccctgt cccacccacg ataataggca actttccacg
    23401 actagtaatg tctgcaatta aggccttggc ttccgctaca aattcataag ccgaatagcc
    23461 ctcggtcaca tctcggacat caatcaagtg atgaaccgca gcagcctgct cttcaggact
    23521 agcttttgcc gtgccaatat caagtttgcg atagacttgc tggctatcac cgctaataat
    23581 ttctccattg tagcgctggg ccaagtctat gccaagggca gtttttccta ccgctgtcgg
    23641 tccaatgacc acaataactt tagttttcat ctttttcctt gaaaatattt tgttttttcg
    23701 ctaaaatcta ttataacaca agtaaaggag aaataactat ggctaaaggt tttggtaaag
    23761 gtttcttgac aggtgttctt tctacagttg ctcttgcagc aggcgcagtc ttcactgttc
    23821 acaaaacaat cattgaacca gaagaaaaga aagaagcttt catcgaagaa aatcgcaaaa
    23881 aagctgcacg tcgccgtgtg gcacattaaa aagctggcaa atgtctagct tttttgtttg
    23941 acaaaaaatc ccttagaagc tctgccattt tcacagaaaa atgccagcct tcttaaggga
    24001 ttatacttta taagataata tcttcttttt caatgagaac aatagtccac atcatggcaa
    24061 gtgtgacgat agccagagca agaatcaatg gcatccagtt tccgaagatt ggatagctgt
    24121 aaccgagaag gcctggtcca aatgctgcaa tgagatagcc acctgtctgc accattcctg
    24181 ataactgggc agtagcctgg ctgttgcttg ttttcaaagt aaagctcaac atcatataag
    24241 ggaagagggc tgcatttgaa aaacttaaaa taatgtgaag cgctgaccaa agaatgagat
    24301 tggtcggaat caataccatc atgaccaaac caagtaaggt aacagatgaa atagccagca
    24361 tgatatttcg acgcatttct tttgtctgac gggaaagaac agctgggata atcatagaca
    24421 tgggaatagc tgtcatattg aagaagccag ccattagccc tgcttctgcc ttactaaagc
    24481 caactgattg agaaatagtt ggcaaccagg taatctctgt atagtagagg actgattgta
    24541 gcccaccaaa tatcaaaaag gcaatcgctg ctttattttt ccagatagat gatttctggt
    24601 ttccttggtt ctcacttgca aatcgatggt tatttcttac attaggcaac cagattagga
    24661 aagccataaa caccaatcct gtaattaata gaataaaaaa ttcccaagaa ctagaggata
    24721 caattggaac agcaatcata gatgcaactg ttgccgccac ccccatgaga gtgatataaa
    24781 tcgtcgtata cagaccaatt ttctttggaa aattagccgc aaccaaactt ggtagcaaaa
    24841 cattaataaa tgctattgta gcaccaacaa gcattgtacc aatgtaaagg gctggtaagt
    24901 tgagaactcg cattcctgaa cccagcacca taaccagtaa gactagggcc attagttttt
    24961 ccatgccaaa cttagccgca agacgcggtg ctaaggatga acagagagca aacatgatga
    25021 gcggaataga ggttaaaatt cctaatgaac tcacttctac tctcagtcct gctgccacat
    25081 ctatcaaaat agcaggcaaa gctgtaaagg gggcacgcat cacgacccct agcatgacga
    25141 ttcctgcaat tataaatggt gattgttttt tcatttttct cctaagtctc ctaatgttcg
    25201 gatttcagca cttttgatta taccatagtt gacaaatgat agaaagttat ttccttctat
    25261 tttttcaagt tatagttttt aactatggat ttgccatgaa aataactatt tttcaaatga
    25321 tagcttatga gttagaatag gttcatcact aaaacgaaag aaagagagat acatatatga
    25381 caacttcaag attccaatta gtcggttcac ttcttcgacc agcaaactta ggagaattta
    25441 aacgacaaat cgaagctcgt gaagacatca aatacccatt ctatgatgac ttcgatggtt
    25501 acaaagaaac tgaagcttca ctcatccaaa aaattgttgc agaacaaaaa gaaaacggtt
    25561 tggatattgt gacagatgga gaatttggtc gttcaatgtg gcacctagat tttctctggg
    25621 gcttcgacgg tatcgagcgt tacattgctg agcatggtta tccatttaaa gaccatgatg
    25681 gacagatttt tgaaacacgt aaggatattg gattgcgtat tactgctcca ctttctgcaa
    25741 aaaatcacca tttcattgat attttcaaac aagtgaaaga attagcagga gaaactgtaa
    25801 ccaaacaaac tattttcggt ccagcccatg cctttaatga attgacaatt ttcaatggac
    25861 aagttggacc aaatcaggtc tacaagactc gtgatgaatt aaaagtcggg ctcatcgctg
    25921 cctataagga atttttagac cagtacaagg aagctggtgg acaaatcgtt caatttgatg
    25981 actgcctttg ggaattattt gaccctgcta accctgtacc tttcttgcca caagacgatt
    26041 ctgaagcatt ggccgctttg gctgacgagt ttgttgctat caataatgct gttattgatt
    26101 acggacatga aattggcttg accgtctgga cgcacaactg ccgtggtaac tatgaaagcc
    26161 gctctgcagc tggcggaact tacgaagcta ttgccgaaaa attcctgcgt gaccaaaaat
    26221 acgatcgatt cttccttgag tgggatgatg aacgtgcagg tgatttaaaa gctttggaaa
    26281 gcttgcgtga caagaatgtc gaagtcgttc ttggtctact ttcaagtaaa acttctgatt
    26341 tggatgatga agaacgtgtt tacaaattac tcgaggaagc aagcaagatt attccaaaag
    26401 agcgtctcta cttgtcacat caatgtggat tcgcttcatg tgattcaggt aatgaattag
    26461 ccatcccgca acagtgggca aaaattaaac aaggtcaaga aattgcagag aagttttggt
    26521 cttaagccac ttcttgttat tcgtgcataa aagccctgtc atcaatcagt gtctctaagt
    26581 taactctgat cagttaatct atgatatcta atcccaagta tttttccgat gtaatacttg
    26641 ggattttatg ttcggacaac acaaaaggag actttttata tagtctacag gtttatcaca
    26701 attggccacg agggtttcat acttatcggt gccgaacatg cgaaaagaaa gggagtaaaa
    26761 ctcggtttca tgctgctttt cccatctcga atccgaatca gatagaacca gcctttttat
    26821 tggtaacaat ccataaagtt atagttttta tgacctctat ccgctacaat gagggctggt
    26881 tcgaaaatgt cgttaagatt taatctctaa tccaac
//
LOCUS       DASFSW010000010        26303 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.010, whole genome shotgun sequence.
ACCESSION   DASFSW010000010 DASFSW010000000
VERSION     DASFSW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26303
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.010"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            95..922
                     /locus_tag="U1099_000368"
     CDS             95..922
                     /locus_tag="U1099_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEM4866717.1"
                     /translation="MKSWVENVTIKKYEDRSGLTGLKQGPLVWTEVFLREVNRQSNTG
                     ILHFWPMDQTVILGMMDSQVAQLDKGLASIGQAGYSPIIRSLGGLAVVADDGILNVTL
                     IFPNPSGHKVDLRESYQVMVDLIAQALSDFPFEVVSGEVATSYCPGTYDLSIKGRKFA
                     GLAQRIYQEAIAISAYISVSGNQVKRGQVVADFYAASFAPQEVSDRFPQVNPNSMANL
                     SDLVAQDLTMEDMKIRIEHVLIENGAFLTTFYPSSDNMADFMALGKTIKQSMEKYGI"
     gene            932..2380
                     /locus_tag="U1099_000369"
     CDS             932..2380
                     /locus_tag="U1099_000369"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEM4866718.1"
                     /translation="MTWKDATAMAQAVNQKQVSAKELVQETIDRIEKLNPTLNAVVSK
                     QYEEALKEAEKEDYLGKPFAGVPFLLKDLGQNEKGQPSSAGSRLLAGRPAGHTDTYVQ
                     RLKDLGFIIVGRTNTPEFGFKNISDASLHGPVKLPLDPSRNAGGSSGGAAAALASGMV
                     SIAAASDGGGSIRIPASFNGLIGLKTSRGRIPVGPKSYRGWQGASVNFALTKSVRDTQ
                     RLLYHFQDYQLEAPFPLAKLSEEEVFGKLSRPLKIAYYTKSPVGSKVSPEAVEAVQKA
                     CHHLANLGHEVVELSEYPLDGVALMKSFYLMNSVDTAQMFDEIEAAFGRQMTLDDMEV
                     MSWAIYQSGQTIPAKLHSKALLDWDQFGASMARFHEEYDLLLTPTVADVAPKHGQFDL
                     SADLLDRLKQTQNYSMEEQQDLIWQMFEDSLALTPFTQQANICGQPAISLPTYVRADG
                     LPIGIQLTASKGREDLLLRLADQMEAAGLLNL"
     gene            2505..3380
                     /locus_tag="U1099_000370"
     CDS             2505..3380
                     /locus_tag="U1099_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM4866719.1"
                     /translation="MLKKVLASALLVSTLTLAACSSSSSNTSSSSAEQTQWEKVQEAG
                     VLKVATPGTLFPTSYYNDAKELVGYEIDMINEIGKRLKLEIEYQEIGVAEAFTAVDSG
                     KVDISVNNFDMTEARKEKYNFSIPYKYSVGGYIVREDGSSGIEAADLSDWTGKKAGGG
                     AGTQYMKIAEKQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVMAIKWANANFP
                     DIKVKMGDAKYNPTEQGIVMSKADTSLKEKIDEALEAMKADGTLKAISEKYYAGQDLT
                     VPVENADQLPVIEVE"
     gene            3471..4142
                     /locus_tag="U1099_000371"
     CDS             3471..4142
                     /locus_tag="U1099_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM4866720.1"
                     /translation="MDINWQAVFNADIAREAIPQILEGLPYTLSLSLIGFALGTFCGF
                     FVAFMRMSKFGPLRWLAMAHISLMRGIPLMVLLFFIYFGLPFMGLQLDAISASIIAFT
                     SMSSAYISEIIRASLSAIDKGQWEAARSLGLRTNVIYRKIIIPQAFRIALPPLSNVLL
                     DMVKSTSLTAMITVPEIFNKAKIVGGAKSDYMTVYICVALIYWAICTLYAFGQLRLEK
                     RLATY"
     gene            complement(4179..4649)
                     /locus_tag="U1099_000372"
     CDS             complement(4179..4649)
                     /locus_tag="U1099_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866721.1"
                     /translation="MKKYISLIALCMMIPCLSACHSVTSEVNKEMQNELTAASIETEK
                     ALKTDERLAEIESFQQIVEIAASKGEFSLESSFSGKVYLMATKENLEKLSKEEFTLNK
                     KSFSTIDAGSSNDFNIRYKHQTYFTVQSLSVQDMEDFEYTKSSSTSAILVFYPN"
     gene            complement(4661..5167)
                     /locus_tag="U1099_000373"
     CDS             complement(4661..5167)
                     /locus_tag="U1099_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEM4866722.1"
                     /translation="MNKKIFFNIVIQLLTFIRHGLIALTGLIMLALLILLIGGKPIQN
                     SLQLDVPLPIIVIILIISILLVACFIYMATILKKLLINFQTGTIFSTENVVYTKIILG
                     TLIIWTSIQFASAFFLSYLDLENVSDLYDFSLSDYFINGVLIIIALLAKMVLEKGVLL
                     QAENDEII"
     gene            complement(5169..5384)
                     /locus_tag="U1099_000374"
     CDS             complement(5169..5384)
                     /locus_tag="U1099_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4866723.1"
                     /translation="MEMIRVNLDKVLKDRQMTSKDLAEQVGITEANLSILKTGKAKGI
                     RFNTLMSICRILDCQPGDILEYVKEGE"
     gene            complement(5386..5910)
                     /locus_tag="U1099_000375"
     CDS             complement(5386..5910)
                     /locus_tag="U1099_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866724.1"
                     /translation="MKTNLLKTAGLLAGCVRYFFYLLIAGYIFFSLAGFRGDSFVIGS
                     WEYTFNKPIDQISVWLILLTSGLILIILAVLSHIAHLIQKLCKLLLEEDYFASASLEL
                     YQKLFISLLVLTAGQFCLTSLITLTNAAGPNNFLNLRWSDFILNALFIFLTYFVWTLV
                     QKGQQLETENSEFI"
     gene            complement(6045..7913)
                     /locus_tag="U1099_000376"
     CDS             complement(6045..7913)
                     /locus_tag="U1099_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEM4866725.1"
                     /translation="MTLVTNLKQHSNIITTALCLALILIGILLFQTGQGWAPILFISA
                     FVIGGYQSAKEGISELIFDKHLSVDLLMILAAIGSGLIGYWMEGALLIFIFSLSSTLE
                     ELAMEKSKNAIAALMNMTPPTARKIEENGDITVLDTAAIRIGDLLQVRKGDTVPLDAT
                     LISPQSIFDESMITGEPLPAEKMTGAAVIGGTINQGPTVTVQVTAEKGDALFDKIVQM
                     VENAQESKSKTATFIENMEDTYVKIVLVVVPLFILFAHFALCWDWLTAFYRGMILLTI
                     ASPCALVASSSPATLSAISRAARKGMIIKGGDIADNIANLEAIVFDKTGTLTIGKPEV
                     VDATYLGDEKLIREVVQAVEKQSSHPIAQALMTYTADSSAIALQSLEDVTGKGLVAGY
                     EGDSWKIGKAGFVVDSLVSPLSAGLLAQIDEAESTGKTLVYVSQNDVLVAIFMVEDSL
                     KPESKQLIAQLKEMGVTPILLTGDQEKTARYVASQVGIDRVIANCLPTDKAAVIQKLQ
                     TEFASVGMVGDGINDAPALAQANVSYAMGSGTDIAMESADIVLMEDLTRIPYSIRLSK
                     KMRGIIKQNIIFALSVIALLIVSNLFQSINLPLGVVGHEGSTILVILNGLRLLYFK"
     gene            complement(8075..8530)
                     /locus_tag="U1099_000377"
     CDS             complement(8075..8530)
                     /locus_tag="U1099_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM4866726.1"
                     /translation="MELHSHFNAENQTAFEHVIQHLKEKGVRITETRKAVVAYIIESD
                     DHPSAEMIYQDLLPNYPNMSLATVYNNLKLLLEEGFVTKLKRTNDNTTYYDFMGHEHL
                     NVICEVCGKITDFMDVEIPSLKKEAHTQTGYKITKEVLSIYGICPDCQG"
     gene            8697..9446
                     /gene="truA"
                     /locus_tag="U1099_000378"
     CDS             8697..9446
                     /gene="truA"
                     /locus_tag="U1099_000378"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEM4866727.1"
                     /translation="MTRYKAIISYDGHDFSGFQRQTHARTVQEEIEKTLVRLNSGQPV
                     TVHGAGRTDAGVHAYGQVIHFDLAGSRDVEKLRFALDTQTPEDIDVVQVEQVADDFHC
                     RYAKHSKTYEFLVDIGRPKNPMMRHYATFYPYDLDLSLIEEAIQDLVGTHDFTGFTAS
                     GTSVEDKVRTIKAASMEYDQRRQFLIFTFSGNGFLYKQVRNMVGTLLKIGNGRMPVGQ
                     IKRILAEKDRGLAGPTAAGNGLYLKEIIYED"
     gene            9436..10197
                     /locus_tag="U1099_000379"
     CDS             9436..10197
                     /locus_tag="U1099_000379"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEM4866728.1"
                     /translation="MKTKYILALSGNDIFSGGGLHADLTTYTVHGLHGFVAVTCLTAM
                     TEKGFEVIPTDAAVFAQQLASLKDVPFSAIKIGLLPTVDIAEQALAFVKAHQEIPVVL
                     DPVLVCKETHDTEVSQLRDELLKFLPYVSIITPNLAEAQLLLQKDIETVEEMKQAAVA
                     LYDLGAKAVVIKGGNRLGGSEAIDVFYDGQEVVVLQSPLLSENNVGAGCTFASSIASQ
                     LLLGREPFEAVRLSKDFVYAAIEKSDDYGVIQYEK"
     gene            10187..10672
                     /locus_tag="U1099_000380"
     CDS             10187..10672
                     /locus_tag="U1099_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM4866729.1"
                     /translation="MRNKKTQELVLLAILTALSLVLAFVYVPTATGFVTLLDVGVYFT
                     AFYLGKKEGAIVGGLAGLLIDFLLGYPQWAFFSLLFHGAQGYFAGWTGKKRIFGLVLA
                     TLSMVGGYYLASRIYFNDLKAIESVLSNTLQNTVGLVVGYILAQAIERIGAVNHVIKG
                     N"
     gene            10653..11219
                     /locus_tag="U1099_000381"
     CDS             10653..11219
                     /locus_tag="U1099_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEM4866730.1"
                     /translation="MSLREIKDQTQQVVQEVLELSNLQKGQIFVLGLSSSEVIGGHIG
                     KNSSLEVGEVVVETILEILDQKGIYLAVQGCEHLNRALLVERELAIQKDLEIVNVLPT
                     LHAGGSGQLAAFKYMKDPVEVEFITAQAGVDIGDTAIGMHIKHVQVPIRPSLREIGQA
                     HVTALASRPKLIGGVRAAYQEDKIRKGS"
     gene            11219..11833
                     /locus_tag="U1099_000382"
     CDS             11219..11833
                     /locus_tag="U1099_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEM4866731.1"
                     /translation="MKEFTVKDLVWTRPPRQFTISDEEITITTHPQTDLWQKTYYHFV
                     NDNAPVLQMETEEDYFSFTVKTDFAGSHTRFDQCGIVLYQDSENWLKCSIEYENDDFQ
                     HLGSVVTNHGFSDWATTEISSQVKSIWFRLSRRGADYCFENSEDGIHFKQMRVCHFHQ
                     GTGKIAFGLYACSPENSSFTARFTNMELSDCKWLPHDGQEPDEI"
     gene            12106..12537
                     /locus_tag="U1099_000383"
     CDS             12106..12537
                     /locus_tag="U1099_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEM4866732.1"
                     /translation="MDTKFSVALHILTMISESKEILSSQALAESVGTNASYIRKVIAL
                     LKNAGLITSHQGRSGYQLSKSPKDISLLEIYLATQEVEHIRLFQIHQNANLTCPVGQH
                     IEGAMVPIFSSVEQDLENQLSRQSLDNVIDNLYQSAQETRI"
     gene            12617..13618
                     /locus_tag="U1099_000384"
     CDS             12617..13618
                     /locus_tag="U1099_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEM4866733.1"
                     /translation="MKAAQHTSYNKNKITLNLTEIAKPQIKPNQVLVKVTAAGVNPLD
                     NMISRGDVKLIVPYKLPQTAGNELVGLIEEVGSSVTTFVAGDRVFGRLPLDSIGAFAE
                     YVAVDAQALAKVPAYLTDEEAAAVPLTALTIMQALDLMGAEAGKTIFISGGTGGVGGM
                     AIPIAKAKGLTVITNGDGASGERVLKLGADRFIDYKTEDYTKTLSNVDYVLDTLGGAE
                     TEKQMSIMEKGGQLVSLRAMPNGEFAKRMNLPKWKQILFGLAGRSFDKMAKKYGVHYH
                     FIFVESNGRQLQEVADIFSKLEIKPSIDTVYPFEEVNAALDKVANGRSRGKTVLSFN"
     gene            13644..14489
                     /locus_tag="U1099_000385"
     CDS             13644..14489
                     /locus_tag="U1099_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM4866734.1"
                     /translation="MSYITTKNQYITVSNNKIAYRELSKGKSDIPLVMLVHLAATLDN
                     WDPKLLDLLAEQHHVIVLDLPGVGASQGKVAPTIPGMAEQAVSIIKALGYEKINLLGL
                     SMGGFIAQEIVRLDSQLVNRLILAGTGPRGGFEVDKVTGKTFRYMLKAGLERVDPKRY
                     IFYNHDEAGRLEAEKVLGRMGQRSAAHADKDMNVPGFLTQLKAIKRWGREPQDDMTFI
                     TQPTLIVNGDKDMQVPTENSYTMHEKIKNSQLIIYPNAGHGSIFQNVEDFSKALLAFL
                     EETNA"
     gene            14482..15351
                     /locus_tag="U1099_000386"
     CDS             14482..15351
                     /locus_tag="U1099_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEM4866735.1"
                     /translation="MPKTILITGSTDGIGKHLAMKLASEGHEVILHGRNSEKFRVALS
                     DIQLKTGNKKIHGYLADFSKLADVYRFSQEIKRDFEQIDVLVNNAGAYFGDARVATAE
                     NIEMTFMLSVQVPYILTTELLPLLEKAVGRVIHTSSFMHHFAQTRGLDFGLEQSYSAA
                     MAYNNAKLYTIWLAIAQAEALEKQGSSVTVNAYHPGLIATNLGNDGVKRNLRSRILTS
                     LMKPFSKDLDQGIETGYYLSLSPEVAGQSGRYFAEKKVAKVSLKGFDVPKSQALLAYL
                     DKKIQVFKESYRD"
     gene            15344..16555
                     /locus_tag="U1099_000387"
     CDS             15344..16555
                     /locus_tag="U1099_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HEM4866736.1"
                     /translation="MTKILLENLQLPNGQVLDNRFFKSAMSETMADSQGAPSDDLIAL
                     YDFWAKQGMGVLVTGNIMVDGRYLGEPGNVVLDSDDHLAQFRKWAAVGKKNGVPIWLQ
                     LNHPGKQMYRSINQTPIAPSAIPISGGSASAFRPPREMTVFDIKEARDKFIAAGLRAK
                     EAGFTGVQLHAAHGYLINQFLSPADNQRTDRYGGSLDNRMRFLVEVYQGLRDKVGPDF
                     TIALKLNASDFKEEGFGFEDCKHVVKTMSDLGIDLIEISGGNYETPVFGSEYENGAGF
                     VTYALALADLTSVPIVSTGGFRKVTQMEEAIKDGVSMIGLARPFVLRPSLVNDYRQVG
                     DVQLTTPRLTTGLPKLDKALGPIIGVSYYEAQMKRLAMGKSVTVSQHAWPYLLQTIKA
                     HGLSALKPRRK"
     gene            complement(16822..17331)
                     /locus_tag="U1099_000388"
     CDS             complement(16822..17331)
                     /locus_tag="U1099_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEM4866737.1"
                     /translation="MERVKRKESLFVESVVEALWELLEDKSFEKISVSELVERAGIGR
                     VTFYRNFSNKEEVLERSFNMELQAWLSERQIDLSDWSDAHLLLALRQFFDFWYEQQDK
                     IRLLTTNHLDYLLEEVLDRYFKERLGDLTDDFHLQFIVGGFFRVLKTWVARGCKESPD
                     DIMRLMGEQ"
     gene            17474..18592
                     /locus_tag="U1099_000389"
     CDS             17474..18592
                     /locus_tag="U1099_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase"
                     /protein_id="HEM4866738.1"
                     /translation="MKKVWKIVAAVTVVLALLGFIAYKKTFGWSAPELVAQTPQVNEW
                     YRLSPEGVVDSQGNQAHGLIRTGKEKNKVMVYFFGGGVSINEETASGGTRYFATTTGH
                     QDYVATWGIGSPQEDNPFKDWTMIVLPYGTGDFHAGTQNFSYVDDNGKEQVVHHQGYS
                     NLMSILAAAKPHVGNPDTLLVTGFSAGGFATSLLADDVISHFPTAKNVTVAVDSSFLR
                     HDNWKSIAENVWKTPTSISDRLHSDNIVLDSLVALHEKRGDSVKILFDISYRDGILQQ
                     YQAYIDQGKLADATEESSDHFQKELKKMIQELQSKIDGVGIFIWNYGQDEKTTATQHT
                     TINFSTFFTPMSKDKSVAEWLDDAVNGQVNSYGLELLE"
     gene            18602..19621
                     /locus_tag="U1099_000390"
     CDS             18602..19621
                     /locus_tag="U1099_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM4866739.1"
                     /translation="MKKFKKIILWTLGIVASLGIIAYVSVTNHPQLVVGVIQSFMYKE
                     SSPNSYSPLYDPIDGLKENGQYLKSEIAYSKDYPNSFLDITYPDQNLEADRPTLFYFH
                     GGGFFGGSKNMGDPLAASQATYLIDDIVSKGYNVVNVDYALVPDYHFPTPVIQMNQAI
                     AYMKEHAEEYHLNMDNVVLMGSSAGAIMVAQYGSLLANNDYAQLLNIEPSIAKEHVKA
                     LVIDDAPLDYAKFSLSTKLLVGNYVSETIYLSEEEINAYNPISHVNKDYPASFLLGSE
                     YRTDMVSLNKALKQAGVENELVDPLAEEGLEKPHGFVANLRTDPVSAKAFERLITFID
                     DRTKE"
     gene            19843..21126
                     /locus_tag="U1099_000391"
     CDS             19843..21126
                     /locus_tag="U1099_000391"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEM4866740.1"
                     /translation="MSVSFEAKETNRGVLTFTIGQDAIKPELDRVFNKVKKDINLPGF
                     RKGHLPRAVFNQKFGEEALYQDVVNALLPAAYEAAVAEAGLEVVAQPKIDVVSMEKGQ
                     DWTITAEVVTKPEVKLGDYKNLAVSVEATKEVTDEEVDAKIEAARNNLAELVIKEGPA
                     AEGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEAQLVGHAAGEEVNVEVT
                     FPEDYQAADLAGKPALFVTKIHEVKAKEVPALDDELAKDIDEEVETLDELKAKYRKEL
                     EAAKEVAFDDAVESAALELAVENAEIVELPEEMIHEEVHRAINEFLGGMQQQGISPDM
                     YFQITGTTREDLHKQYEADAEKRTKTNLVVEAVAKAEGFEATEEEINKEIEDLAATYN
                     MEVAQVRSLLSPEMLKHDIAVKKAVEVITSTATVK"
     gene            21177..22061
                     /locus_tag="U1099_000392"
                     /pseudo
     CDS             21177..22061
                     /locus_tag="U1099_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            22171..23091
                     /locus_tag="U1099_000393"
     CDS             22171..23091
                     /locus_tag="U1099_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEM4866741.1"
                     /translation="MLKKVIRGCFVALFGFVLAACSAQKEASQVQPGMKIVTSFYPIY
                     SLVKEVSGNKNDVRMIGSRQGIHSYEPSAADIKAIYDADVFIYHSRILESWAGRLEPN
                     LQGSSVKVLEASTNLPLTKVPGLEDMEAGQGIDEASLYDPHTWLDPVLVGQEAVSIGE
                     LLAESDPKNADYYRQNAATLEEKAQKLADKYSPIFLKATSKTFVTQHTAFSYTAQRFG
                     LKQLGIAGVSEEEPSPRQLAEIKEFVDTYNVQTIFTEKGASDKLAKALASSTGVDLKV
                     LDPLEADPENNLTYLENLEQVLETLAQELK"
     gene            23125..26280
                     /locus_tag="U1099_000394"
     CDS             23125..26280
                     /locus_tag="U1099_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEM4866742.1"
                     /translation="MKKKAVVGSVAALVLGMSLCSYQLGRFQAMEEQKNRVSYIEDSQ
                     SVQTTVAEQLTPDQVSAKENIDAEQIVVKITDQGYVTSHGDHFHYYNGKVPFDAIFSE
                     ELVMKDPNYVLQDSHIINEVQDGYVIKVDGKYYLYLKDPSKHKNVRSKEEVERQKGIS
                     SADSKNQAAGNSKDGRYRTDDGYVFNPTDVIEDTGDGFIVPHGDHFHFIPKKDLSATE
                     LKAAQDYWNQKGSVSSASGSQYGDRNNRAQQTTISAGQGQDLASLLAQLDATPLSQRH
                     VEADGLVFDPRTITKKTAAGVIVPHGDHYHFIPYSQMSPLEEKISRMIGVNGAGVSSG
                     AQASHSQHTPTQPNRPVTPIGTVTTQPVSPTQPVLPTQPKQSTGKVVSYMGRQIPAYG
                     KGLDGKAYFTSDGYTFSKDSITSVDDQGLIASHGDHFHYVGFGELEDFEIKQVEEWVN
                     EKAGKQVPPKTSEQVGNDAKPTKPSQGNDSKPVKPIQEENRPAFEYKQVTAKRKLAGK
                     VVYEMEVGGKTYTYGRDELDLMKISFAELTLAEKDKQYIFDIAPLAEGDLKPAMLVGM
                     DQIPMKGANATYDTGQSFIIPHIDHIHVLPYTWLSKEQIATIRYIMQHPEIRPSAWTT
                     SGHGDGEATDLVPPILNATPKANRLGLKNWQIIHTAEEVMDARAKGKFATNDGYIFSA
                     EDVLDPASFVFSQAFSLPRATGGSLRSISKKDLSKEELEAVQTLLDKRDAEELAQNVT
                     PIEKRAGLKNWQIVHSAEELAEAKAAGKYTTKDGYIFDPADLLDPKVKIGTDNYRIPR
                     VITDGYRRINKSDLNYLSELIPAEAMVAQREKSNSSSPSTPAPTETGASAGETTKPEQ
                     PQVAKETAEEIYNRVEAKKVVPFEALTYNAGYATEVRNGTLVIPHQDHYHYVSFKWFD
                     QGSARSPEGYSLEDFLATVKYYMTNPQERPVSDDGWGVFTPNTPSESTEETETEESDE
                     EIISEETEEIDEFTEELKRRAEEFGMDFKTFEQSLVTLSDRYKVSFEAFEYDAASKVV
                     RLVDKDGVKRTISLPSLEEQV"
BASE COUNT         7699 a         5358 c         6093 g         7153 t
ORIGIN      
        1 ttctttttca gaaaatcctc tgggcatttt ttccgacgca tcacaggctc tgtgttaaaa
       61 taagatatag taatttgaaa gagaacgctt acgaatgaaa tcttgggttg agaatgtgac
      121 tataaagaaa tacgaagatc ggtctggttt gacgggtttg aaacaggggc cactggtgtg
      181 gactgaggtc tttttgcgcg aggtcaatcg tcagtccaat acagggattt tacatttttg
      241 gcccatggac caaacggtca ttctggggat gatggacagt caggttgccc agctggacaa
      301 gggcttagcc agtattggtc aggcaggcta cagtcctatt attcggagtt tgggcggttt
      361 ggcagtcgtt gcggatgacg ggattttaaa tgtcactctg atttttccca atccttctgg
      421 tcacaaggtt gatttgcggg agtcttatca ggtcatggtg gatttgattg cccaggcgct
      481 ttcagatttt cccttcgagg tggtcagtgg tgaggtggca acttcctact gccctggaac
      541 ctacgacctt agtattaagg ggcggaagtt tgctggattg gctcagcgga tttaccaaga
      601 ggctattgcc atttctgcct atatcagtgt atcaggcaat caagtcaaac gtgggcaagt
      661 ggtggctgat ttttatgcgg caagttttgc tccacaagaa gtatcggaca gatttccgca
      721 agtaaatccg aattcgatgg ccaatctgtc agacttggtc gctcaggatt tgacgatgga
      781 ggatatgaaa atacgaatcg agcatgtgtt gattgaaaat ggggcttttc tgaccacctt
      841 ttatccttcg tcggacaaca tggctgattt tatggcgctc ggaaagacca tcaagcagtc
      901 tatggaaaaa tatggtatct aggagattct tatgacatgg aaagatgcga cagccatggc
      961 tcaggctgtc aatcaaaaac aggtttctgc caaggaattg gtgcaagaaa ccattgacag
     1021 gattgaaaag ctcaatccca ccctcaatgc ggttgtcagc aagcagtatg aagaggcctt
     1081 gaaagaagct gaaaaagaag actaccttgg caagccattt gctggagttc ctttcttgtt
     1141 gaaggatctg gggcaaaatg aaaaggggca gccatcttct gctggttctc gcctgcttgc
     1201 tggtcgacca gcaggccata ccgataccta tgtccaacgc ttgaaggact tgggtttcat
     1261 cattgtcggt cgaaccaaca cgccagaatt cggcttcaaa aatatctcag atgccagcct
     1321 gcacggtcct gtcaagcttc ccttagatcc tagccgcaat gctggtggat caagtggtgg
     1381 tgcagctgca gcactggctt caggcatggt ctctattgct gcggcatcgg atggtggtgg
     1441 gtctattcgc atcccagcct ccttcaatgg cttgatcggg ctcaaaacca gtcgtggtcg
     1501 gattccagtt ggacctaagt cttatcgtgg ctggcaggga gcatctgtca attttgccct
     1561 gaccaagtct gtccgtgaca cccagcgtct gctctatcat tttcaggact accagctgga
     1621 agcccccttt ccccttgcca agctgagcga agaagaggtg tttggaaagt tgtctcgccc
     1681 cctaaaaatc gcctactata ccaaatctcc agtagggagc aaggtcagtc ctgaagcggt
     1741 agaagcggtt caaaaagcct gtcatcactt ggcaaatttg gggcatgaag ttgtggaatt
     1801 atcagaatat cctctggatg gagtggccct gatgaaatcc ttctacctga tgaacagcgt
     1861 ggacacggcc cagatgtttg acgaaatcga agcggctttt gggcgccaga tgaccctgga
     1921 tgacatggaa gtcatgtcct gggctattta ccaaagcggg caaaccattc cagccaaatt
     1981 gcactccaag gccctattgg actgggatca gtttggagcc agcatggcgc gtttccatga
     2041 ggaatacgac ttgctcctga cgccgacagt ggctgatgta gcgcccaagc atggacaatt
     2101 tgacctatca gcagatttgc tggaccgcct caagcagacc cagaactatt ctatggaaga
     2161 gcagcaagac ttgatctggc aaatgttcga ggacagccta gccctaactc cctttacgca
     2221 gcaggccaat atttgtggtc agccagccat ttctctccct acttatgtgc gggctgacgg
     2281 cttaccaatc ggcatccaat taacagcatc caagggacga gaggatttac tcttgcggtt
     2341 ggcagatcaa atggaggcgg caggtttact caacttgtaa gggaattcat tttttcagtt
     2401 caattatttg ccaaaacgtt ttcaaaatgt taacctataa aagttggtgg gttaacaccg
     2461 aaaaagtaaa aaaggaagct atagcttcca aaggagattg ttttatgttg aaaaaagttt
     2521 tggcctcagc tctcttggtt tctactttga ccttggcagc atgttctagc tcaagttcta
     2581 atactagcag ttcttctgct gaacagactc aatgggaaaa agtacaagaa gcaggtgtct
     2641 tgaaggttgc aacaccaggg acccttttcc caacttctta ctacaacgat gctaaagaat
     2701 tggtcggata tgaaattgac atgatcaatg aaattggtaa acgtttgaaa ttggaaatcg
     2761 aatatcaaga aatcggtgtg gcagaagcct ttacagctgt ggacagtggt aaggtcgata
     2821 tttctgttaa caactttgac atgacagaag cacgtaagga aaagtataac ttctctatcc
     2881 cttacaagta ttcagttggt ggctacatcg ttcgtgaaga tggttcatca ggaatcgaag
     2941 cagctgactt gagcgactgg actggtaaaa aagctggtgg tggcgcaggt actcagtaca
     3001 tgaaaattgc tgaaaaacaa ggagcagagc cagttatcta tgacaacgta accaatgatg
     3061 tctatttgcg tgatgtatca acaggtcgta cagacttcat tccaaacgac tactatacac
     3121 aagtcatggc catcaaatgg gcaaatgcta acttcccaga tatcaaagtg aagatgggtg
     3181 atgcaaaata caatccaact gaacaaggta ttgtcatgag caaggctgac acaagcttga
     3241 aagaaaaaat tgacgaagct cttgaagcga tgaaagctga cggaactttg aaggcaattt
     3301 ctgagaaata ctatgcggga caagatttga cagtccctgt tgaaaatgca gaccaattgc
     3361 cagttatcga agttgaataa gacctcgtaa caaattaaat gcatcttgta tagactttgt
     3421 cagtcacagg ctggcaaggt ctatctggtt tttaggaaag aggagaagat atggatatta
     3481 actggcaggc agtttttaat gcagatattg caagagaggc tattccacag attttggagg
     3541 gacttcccta cactctgtcc ctatccctaa ttggttttgc cttggggact ttctgtggat
     3601 tttttgtagc cttcatgcgg atgtctaagt ttggcccctt gcgttggttg gctatggccc
     3661 atatttctct catgcgtgga attcccctca tggtccttct ctttttcatc tattttggat
     3721 tgcctttcat ggggctacaa ctagatgcta tctccgcatc tattatcgcc tttacctcca
     3781 tgtccagtgc ctacatttct gaaattattc gtgcatctct ttcggccatt gataagggac
     3841 aatgggaggc agcgcgttcc ctgggcttgc ggaccaatgt gatttacaga aaaatcatta
     3901 ttccccaagc ctttcgcatt gcccttccac cgctgagcaa tgtcctctta gacatggtca
     3961 aaagtacctc tctgacagcc atgattacgg taccagagat tttcaataag gccaagatag
     4021 tcggcggagc caagtctgat tacatgacag tttatatctg tgttgccttg atatattggg
     4081 ccatctgcac tctctacgcc tttggtcagc tcaggttaga aaaacgcctg gcgacttatt
     4141 aaaaaattcc tcggttcttt tgaatcgagg aattttttct agtttggata gaacacaaga
     4201 atagcggaag tggaagagga tttggtgtat tcaaaatctt ccatatcttg cacggatagg
     4261 ctttgaacag tgaagtaagt ttgatgtttg tagcggatat taaaatcatt gctactacca
     4321 gcatcgatag ttgagaagct ctttttattc agagtaaact cttcttttga gagtttctcg
     4381 agattttctt ttgttgccat taaatagacc ttgccagaga aagaagattc aagagagaac
     4441 tctcctttgg atgcagcgat ttcgacaatt tgctgaaagc tctcaatctc agccagacgt
     4501 tcatctgttt ttagagcttt ttctgtttca atggaagctg ctgttagctc gttttgcatt
     4561 tctttattaa cttctgaagt gacagagtga caagcactta agcaaggtat catcatgcag
     4621 agtgcaatga gggaaatata ctttttcatc atgggctcct ttatataatt tcatcatttt
     4681 cagcttgtaa aagcacacct ttttccaaca ccatctttgc tagtagtgca ataataatca
     4741 gcacaccatt tatgaagtag tcactaagtg aaaaatcata taaatcactt acattctcta
     4801 aatctaggta agataggaaa aaagcacttg caaattgtat gctcgtccat atgattaagg
     4861 ttcctaaaat tatctttgta tagactacat tttcagttga gaaaatagtt cctgtttgaa
     4921 agttgatgag caattttttg agtatggtgg ccatgtagat aaagcaggct accaatagga
     4981 tacttatgat tagaataata acaatgatag gcaaggggac gtctaattgt agactatttt
     5041 gaataggttt cccgcctata agtagaataa gaagtgccaa cattatcagt cccgttaagg
     5101 caatcaatcc atgacgaata aacgttagca actgtatgac gatgttgaaa aatatctttt
     5161 tgttcatgct attccccctc cttcacatat tccaaaatat ctcctggttg gcagtctagg
     5221 atgcggcaga tgctcatcag ggtattgaaa cgaatccctt ttgccttgcc tgtcttgaga
     5281 atggacagat tggcttcggt gatgccgact tgctccgcca agtccttgga agtcatttgt
     5341 cggtctttga ggaccttgtc taggtttacg cgaatcattt ccatatcaga taaactcgct
     5401 gttctcggtt tcaagttgtt gccctttttg tactaaggtc cagacaaaat aagtcaggaa
     5461 gataaagaga gcattgagga tgaaatctga ccaacgcaga ttgaggaagt tgtttggtcc
     5521 agcagcattt gtcaaggtga tcaagctggt taggcaaaat tgcccagcag tcaagacgag
     5581 caaactgatg aagagtttct gataaagttc cagactagct gaagcgaaat aatcttcttc
     5641 cagaagcaat ttacataact tttgaatcaa gtgggcaatg tgactgagta ccgccaagat
     5701 gattaagatt aatccacttg ttaaaaggat gagccaaaca ctaatctggt caatgggttt
     5761 gttaaaggtg tattcccaag agcctatcac aaatgagtcg cctcggaaac cagccaagct
     5821 aaagaaaata tagcctgcaa ttaataaata gaagaaatag cgaacacaac cagctaacaa
     5881 tcctgctgtt ttgagtagat tggttttcat agcaaacctc cgattattat tgttttatga
     5941 taatattatc tgtaaaatta ttataaaaca ataattttta gaatgcaagc gtttttttaa
     6001 aaaaagaata ataagaaaac cgccaagtag ctggcggttt gaagctattt aaaatacagt
     6061 aatctcaacc cattcaaaat caccaaaatc gttgatcctt cgtggcccac cacaccgagt
     6121 gggaggttga tagactggaa gaggttggaa acgataagaa gagcaatgac ggacagagca
     6181 aagatgatgt tctgcttgat gatgccccgc attttcttag agagacgaat ggagtaagga
     6241 attctcgtca ggtcttccat gagtacgatg tcagctgact ccatagcgat gtccgtaccg
     6301 cttcccatag catagctgac attggcttga gcaagggcag gagcatcgtt gataccgtct
     6361 cccaccatac cgactgacgc aaactcggtt tgcagttttt ggataacagc agccttatcc
     6421 gtcggcagac agttggcaat cacacggtca ataccgacct gactcgccac gtaacgagcc
     6481 gtcttttctt ggtcacccgt caagagaatt ggcgtcacgc ccatctcttt caactgggca
     6541 attagctgct tgctctcggg tttcaaacta tcttccacca tgaagattgc caccagcaca
     6601 tcattttggc tgacatagac cagggttttc ccagtacttt ccgcctcatc gatctgtgcc
     6661 agcaagccag cagacagggg actcaccagg ctgtccacca caaatcctgc cttacctatt
     6721 ttccagctgt cgccctcata gccagccacc aagccttttc cggtcacgtc ttctaggctt
     6781 tggagggcaa tagcagagct gtcagccgta taggtcatga gagcctgagc gattgggtgg
     6841 cttgattgtt tttcaactgc ctgaacaact tccctgatta gtttttcatc gccaagatag
     6901 gtcgcgtcta caacttcagg tttaccaatg gtcagggtgc ccgtcttgtc aaagacgatg
     6961 gcttctagat tggcgatgtt atccgcaata tcaccaccct tgataatcat acccttgcgt
     7021 gccgcacggc tgatagcaga aagtgtcgcc ggcgaagaag aagctacgag ggcgcatgga
     7081 gaagcgatgg tcaagagaat catgccacgg tagaaggcag ttaaccagtc ccagcacaga
     7141 gcaaaatgag caaagagaat gaagagtggt accaccacca agacaatctt gacataggta
     7201 tcttccatat tttcaatgaa agtcgctgtt tttgatttgg attcttgggc gttttcaacc
     7261 atttggacga ttttatcgaa gagggcatcg cctttttcag ccgtaacttg tacggtcaca
     7321 gttggacctt ggttaatggt tccgccgata acagcagcgc cagtcatttt ctccgcaggg
     7381 agcggctcgc ctgtaatcat ggattcgtca aagatggatt gcggactgat gagggtagca
     7441 tccagtggga ccgtgtcacc cttgcggact tgcaggaggt cgccgatgcg gatggctgcg
     7501 gtgtctaaga cagtaatgtc accattttcc tcgattttgc gagccgttgg aggcgtcatg
     7561 ttcatcagag ctgcaatagc attcttgctt ttttccatgg ctaattcttc cagagtagaa
     7621 gaaagggaga agatgaagat gagtagagcc ccttccatcc agtagccaat tagaccagag
     7681 ccgatagcag ctaaaatcat gagcaggtcg acagacaggt gcttgtcgaa aatcagctca
     7741 gaaatgcctt ccttggctga ctggtagccc ccgatgacaa aggctgagat gaagaggata
     7801 ggagcccatc cctgtccagt ctgaaacaga aggataccta ttagaatgag cgccaagcag
     7861 agagcggtcg tgatgatgtt gctgtgttgt ttgaggttgg ttacgagtgt catagtttgc
     7921 tcttccttat ttagaattat tatcatttaa ctaagttaat tataacaatt ataaaaagaa
     7981 agtcaaggta tttataataa ttctaaataa gactgttctg ataattcaaa ggaaaataaa
     8041 aaaagcgaca gaaagccgct ttgatattcg tcttttagcc ctgacaatca ggacaaatgc
     8101 cgtagatgga tagcacctct tttgtaattt tgtaaccagt ttgggtatga gcttccttct
     8161 tcaagctcgg gatttctaca tccatgaaat cagtgatttt tccacagact tcacaaataa
     8221 cattgaggtg ctcgtgcccc ataaagtcgt aataagtagt attatcattg gtccgtttca
     8281 atttggtcac gaacccttct tccaatagaa gtttgaggtt gttgtaaaca gtcgccagac
     8341 tcatgttggg gtagttaggt agtagatctt gataaatcat ttcagcacta gggtggtcat
     8401 cgctttcgat aatgtaggca acgactgctt ttctagtttc ggtaatgcgc acaccctttt
     8461 cttttagatg ttggatgacg tgttcaaaag cagtttggtt ttctgcattg aagtgagaat
     8521 ggagttccat agtttcctcc tttcgttatt attttataaa aattctaaga tacaatattc
     8581 agtttcatta tagtgtattt gggaggttgt gtcaattttg agacaggaaa tctgtcatag
     8641 gacagtttgg tgtctcatga cttgtttaca tacttttcct gtgctacaat caaaacatga
     8701 caagatataa ggcaattatt tcctacgacg gacatgattt ttctggtttt cagcgccaga
     8761 cccatgcccg tacagttcag gaagaaatcg aaaaaacgtt agtaagattg aatagtggtc
     8821 agccagtaac cgttcatggt gcaggtcgta cagatgcagg ggtacacgcc tacggtcagg
     8881 ttatacactt tgacttggct ggcagtcgag atgtggagaa gctccgcttt gccctggata
     8941 cccaaacccc tgaggatatt gatgtggtcc aggtggagca ggtggcggat gattttcatt
     9001 gccgctatgc caagcacagc aagacctatg agtttctggt ggatatcggg cgacccaaga
     9061 atcctatgat gcgccactat gcgacctttt atccttatga tttggacttg agtttgatag
     9121 aagaagccat tcaggacttg gtggggaccc atgactttac aggctttacg gcttcgggga
     9181 catcggttga ggacaaggtg cggaccatta aggcggcaag catggaatat gaccagcggc
     9241 ggcagttctt gatttttaca ttttcaggca atggtttttt gtacaagcag gtgcggaata
     9301 tggtcgggac cttactgaaa attggtaacg gtcgcatgcc tgtgggacag attaagcgga
     9361 ttttagcaga aaaggatcgt ggtttggcgg gaccgactgc ggcaggcaat ggcctctatc
     9421 taaaggagat tatctatgaa gactaagtat attttggcgc tttcgggcaa cgatattttc
     9481 agcggtggcg ggctccacgc agatctgaca acctacacag tccatggtct gcatggcttt
     9541 gtggcggtga cttgcttgac agctatgacg gaaaaaggtt ttgaggtcat tccgacagat
     9601 gcggcggtgt ttgcccagca actggctagt ttgaaggatg tgcccttttc tgcgattaag
     9661 atcggcttgc ttcctacggt ggacatagca gagcaggctt tggcgtttgt caaggctcac
     9721 caggagattc ctgtggtctt ggatccggtc ttggtctgca aggaaacgca cgataccgag
     9781 gtcagccagt tgagagatga actcttgaaa ttccttcctt atgtcagcat tatcacacca
     9841 aatctggcag aagctcagct attactccaa aaggatatcg aaacggtgga agagatgaag
     9901 caggcggcag tggccttgta tgacttggga gccaaagcgg tggtcatcaa gggaggcaat
     9961 cgtctgggtg gctcagaggc gatcgatgtt ttctatgacg gtcaggaagt ggtggtgctc
    10021 cagtcgcctc tgctgtcaga aaacaatgtg ggggcaggct gtacctttgc atccagcatt
    10081 gccagtcagc ttttgctggg aagagaacct ttcgaggcag tcagactgtc caaggatttt
    10141 gtctatgcgg cgattgaaaa atcagatgat tatggggtga ttcaatatga gaaataaaaa
    10201 aacacaggaa ttagtattat tagctatctt aacagctttg agcttggtat tagcctttgt
    10261 ttatgtgcca actgcaactg gttttgtcac actcttggat gttggggtat attttacagc
    10321 cttttacttg ggcaagaaag agggagcgat tgtcggcggt ttggcagggc tattgattga
    10381 cttcttgctg ggctatcccc agtgggcttt ctttagtttg ctcttccacg gggctcaagg
    10441 ttattttgcg ggttggacag gtaagaaacg gattttcggt ttagtcttgg cgaccctctc
    10501 tatggtgggg ggctattacc tagcatctcg gatttatttt aatgacctca aagccattga
    10561 aagtgttcta agcaatacct tgcaaaacac agttggtttg gtagttggtt acatcttggc
    10621 tcaggcaata gaaagaattg gtgcagtaaa tcatgtcatt aagggaaatt aaagaccaaa
    10681 ctcagcaagt tgtgcaagaa gtcctagaac tcagcaatct tcaaaaggga cagatttttg
    10741 tcctaggatt gtcctctagt gaggtcattg gtgggcatat tggcaaaaat tccagtctgg
    10801 aagttggtga ggtggttgtt gaaaccatct tggaaatcct ggatcaaaaa ggtatctatc
    10861 tagctgtcca aggctgtgaa cacctcaatc gtgccttgct ggtggagcgg gaattggcaa
    10921 tccagaagga tttggaaata gtcaatgtcc tgccaaccct tcatgcaggt ggttctggtc
    10981 agttggcggc cttcaagtat atgaaggacc cggtggaagt cgagtttatc accgctcagg
    11041 ctggtgtaga catcggcgac acagctatcg gcatgcacat caagcatgtc caagttccga
    11101 ttagacctag cctgcgtgaa attggacagg cccatgtcac agctcttgcc agccgaccaa
    11161 aactaatcgg cggagtccga gcagcctatc aggaagataa gatacggaaa ggttcataat
    11221 gaaagaattt acagtcaagg atttagtgtg gacccgtcca ccgagacaat tcactatttc
    11281 agatgaagaa attaccatta ccacccaccc ccagacggat ttgtggcaga agacctatta
    11341 ccattttgtg aatgacaatg ctcctgtctt gcaaatggaa acggaggagg actatttttc
    11401 ctttactgtg aagacggatt ttgcgggtag tcatactcga tttgaccagt gtggaattgt
    11461 cctttatcaa gattcggaga attggctcaa atgttcgata gaatatgaaa atgatgactt
    11521 ccaacacttg ggaagtgtgg tgaccaatca cggcttttct gactgggcaa ctactgaaat
    11581 cagttcccaa gtcaaatcca tttggtttag gctcagtcgc aggggagctg attactgttt
    11641 tgaaaattcc gaagacggta tccacttcaa acaaatgcgg gtttgccatt ttcatcaagg
    11701 gacggggaaa atagcttttg ggctatatgc ctgttcgcca gaaaactcca gttttacagc
    11761 tcgctttacc aatatggaat tgagcgactg caaatggttg ccccatgacg gacaagagcc
    11821 agatgaaata tagtgagagt cataggtaag tagtaacaga aaaatagttg aaactcatat
    11881 tcttcgaaaa tcaaaaagat ccgttgtcaa cttgccttga agaactcctg ttcaatcttc
    11941 gccttcgttt cctaggcgac ttttgatttt cattgagtat caggaagtaa ctttcgacta
    12001 gtaagagagt tacttttttt atcttttgat ccaaatcctt gccaagaagt ctggcttgtg
    12061 ataaacttgc tcctgtaagt gatacaagaa cagaaaggaa accagatgga tacaaagttt
    12121 tcagtagccc ttcatatact gaccatgatc agtgagagca aggaaatact tagctcgcaa
    12181 gccttagctg agagcgttgg caccaatgcc agctacatcc gcaaggtgat tgcacttttg
    12241 aaaaatgcag gcttaatcac ttcgcaccaa gggcgatcag gctaccaatt gagcaagtcc
    12301 cctaaggata ttagcttatt ggaaatctac cttgctacgc aagaagtgga gcatattcga
    12361 ctatttcaga tacaccagaa tgccaatctt acttgtccag ttgggcagca tatagaaggt
    12421 gcaatggttc ccatcttttc cagcgttgaa caagatctgg aaaatcaact cagtcgtcaa
    12481 tccttagata atgtgattga taatctctat caatccgcac aagaaacccg aatctgacct
    12541 cggtcagatt ttattaactc aaacatagac ctgtaattga tacaggaaca aaccaaagaa
    12601 tagaaaagag taaaccatga aagcagcaca acacacatct tataataaaa acaagatcac
    12661 ccttaacctt acagaaattg ctaaaccaca aatcaaacct aaccaagtcc ttgtcaaagt
    12721 gaccgcagca ggcgttaacc cattggacaa catgatttct cgcggagatg tgaaattgat
    12781 tgttccttat aaattgccac aaacggctgg aaatgaacta gttggcttga ttgaagaagt
    12841 aggttcaagc gtgacgacat ttgtagcagg tgatcgtgtc ttcggccgtc ttccacttga
    12901 tagtatcggt gcttttgctg agtatgtagc ggttgacgct caggctcttg ctaaggttcc
    12961 tgcctacttg acagatgaag aagcggctgc tgttcctttg acagccttga ccatcatgca
    13021 ggcccttgac ctcatgggtg ctgaggcagg caagaccatc ttcatctcag gcggaactgg
    13081 tggtgtcggc ggtatggcta ttccgattgc caaagccaag ggcttgaccg ttatcacaaa
    13141 tggtgacggt gcaagtggtg aacgtgtctt gaaactcggt gcagaccggt ttatcgatta
    13201 caagacagaa gattacacca aaacacttag caatgttgac tatgtcttgg acacactcgg
    13261 tggtgcagaa acggagaaac aaatgtccat catggaaaaa ggtggacagc tggtttccct
    13321 tcgtgccatg ccaaatggtg aatttgccaa acgtatgaat ttgccaaaat ggaaacaaat
    13381 cttgtttggc ctagctggtc gttcatttga caaaatggct aagaaatatg gtgttcacta
    13441 ccatttcatt ttcgtagaga gcaatggtcg ccagctgcaa gaagtagcag acattttcag
    13501 caagctcgaa attaaaccgt caatcgatac cgtttatcca tttgaagaag tcaacgcagc
    13561 actggataag gttgccaacg gtcgttcacg cggaaaaacc gtcctcagtt tcaactaaaa
    13621 gataaaatat acagggaaac atcatgtcat atattacaac taaaaaccaa tacatcaccg
    13681 tttcaaacaa taaaattgcc taccgtgagc tcagcaaggg caagtctgat attcctttgg
    13741 tcatgctggt tcacctagcc gcgaccttgg acaactggga tccaaaatta ctggatttgc
    13801 tggctgaaca gcaccatgta attgtcctag accttcctgg cgtaggagct agtcaaggca
    13861 aggtggctcc gactattcca ggaatggctg agcaggctgt ttccattatc aaggctttgg
    13921 gttatgagaa aatcaatttg ctgggtctgt ctatgggtgg tttcattgct caagaaatcg
    13981 ttcgtctgga tagtcagctg gtcaaccgtt tgattttagc aggcacaggt cctcgtggtg
    14041 gttttgaagt cgataaggtg actggaaaaa ccttccgcta catgctgaag gctggtttgg
    14101 agcgtgtgga tccaaaacgc tacatcttct acaatcatga tgaggcaggt cgtttggagg
    14161 cagaaaaagt cttgggtcgt atggggcaaa gaagtgctgc tcatgcagac aaggacatga
    14221 atgttccagg atttttgacc cagctgaaag ctatcaaacg ctggggaaga gagccgcaag
    14281 atgatatgac ctttatcacc caaccaactc tgatcgtcaa cggtgataag gatatgcagg
    14341 ttccaactga aaattcctat accatgcatg agaagatcaa aaatagccaa ctgatcatct
    14401 atccaaatgc aggacacggc tctatcttcc aaaatgtaga ggatttttcg aaagccttac
    14461 tagccttttt ggaggaaacc aatgcctaaa accattttaa tcactggttc gacggatgga
    14521 atcggcaaac acctagccat gaaactagct agtgaaggcc atgaagtgat cttgcacgga
    14581 cgaaatagcg aaaagttccg tgtagccctc agtgatattc aactaaaaac aggaaataaa
    14641 aagattcacg gctatcttgc agatttttcc aagttggcag atgtctatcg tttcagccaa
    14701 gaaattaaac gcgattttga gcagattgat gtcttggtaa acaatgcagg agcttacttt
    14761 ggtgatgctc gcgtggcaac ggcagaaaat atcgagatga cctttatgct gtctgtccaa
    14821 gttccctata tcttgacgac ggagttgctg cctttgctgg aaaaagcagt aggtagggtc
    14881 attcatacct cttccttcat gcaccatttt gcacaaacca gaggcttgga ttttggttta
    14941 gagcagagct attctgcagc tatggcttac aataatgcca agctatacac catttggctg
    15001 gccattgctc aggcagaggc cttagaaaag caaggttcat ctgtgactgt caatgcctac
    15061 catcctggct tgattgcgac aaatttgggc aatgatgggg tcaaacgcaa cctgagaagt
    15121 cggattctta ccagtctgat gaaaccattt tccaaggatt tggaccaggg gattgaaaca
    15181 ggttattact taagtttgtc accagaggta gctgggcagt caggtcgcta ttttgcagag
    15241 aaaaaagtgg caaaggtcag cctaaaaggc tttgatgtgc caaagagcca agccttacta
    15301 gcctatcttg ataagaaaat ccaagtattc aaggagagct accgtgacta agattttgtt
    15361 agaaaacctc caactgccca atggtcaggt tttagacaat cgctttttca aatccgccat
    15421 gagcgaaacc atggcagata gtcagggagc tccctcagat gacctgattg ccttatatga
    15481 tttttgggcc aagcagggaa tgggtgtcct agtgacaggg aatatcatgg tggacggccg
    15541 ctaccttggt gagccgggca atgtggtcct tgattcagac gatcatctgg ctcaatttcg
    15601 gaaatgggca gccgttggga aaaagaacgg tgtgcctatc tggctccagc tcaatcaccc
    15661 tggcaaacag atgtatcggt cgatcaatca aactccgatt gcacccagtg ctattccaat
    15721 ttctggaggt tcagcttcgg cctttcgccc accgagagaa atgaccgtgt ttgacatcaa
    15781 agaagctaga gataaattta ttgcagcagg ccttcgggca aaagaagcag gatttacagg
    15841 tgtacagtta catgctgcac atggttactt gatcaatcaa ttcctctcac cagcagataa
    15901 tcagcggacg gaccgctatg gtggcagtct ggacaatcgc atgcgattct tggtggaagt
    15961 ctatcaaggt ctgcgtgaca aagtgggacc agattttacc atcgccttga aattgaatgc
    16021 ttctgatttt aaggaagaag gctttggttt tgaagattgt aagcatgtgg tgaaaaccat
    16081 gtctgacttg ggtatcgacc tgattgaaat ttcaggtggc aattatgaga cacctgtttt
    16141 tggcagtgag tatgagaatg gtgctggatt tgtcacctac gccctagcct tagcggattt
    16201 gacttctgtt cctattgttt caacaggcgg tttccgaaaa gtcactcaga tggaggaggc
    16261 tatcaaggat ggtgtgtcca tgattggcct tgctagaccc tttgtccttc gtccaagtct
    16321 tgtcaatgat taccgtcaag tcggtgatgt gcaactgaca acgcctcgcc tgacaacagg
    16381 tcttccaaaa ctagataagg ctcttggtcc cattatcggt gttagttatt acgaggccca
    16441 gatgaaacgc ttggctatgg gaaaaagtgt caccgtcagt caacatgcct ggccctatct
    16501 tctccaaacc atcaaggcac acggtctgtc agccttgaaa ccgaggagga agtagaatga
    16561 tgctaatcat gacgatacta aattaatttt aaagctactt ttacaatgcg tgtatgagta
    16621 gctttatttt cattatagat attcgattga ggagaggttc aagcctaacg ccgcttctca
    16681 agatttgtat tacagtactt gaaatcatta ttaaaaatat ccagagagat gatattatgc
    16741 acaaaaaaga cttagagtga ctgaaaaaca gttgcctaag tctttttgct tggtctggtc
    16801 aagaacattg acaagcttgc tctattgctc acccatcaac cgcatgatgt catcagggct
    16861 ttccttacag cctctagcaa cccaggtttt aagcactcgg aaaaatcctc ctacgataaa
    16921 ttgcaggtga aaatcatcag tcaagtcccc cagcctttct ttgaaatagc gatccaagac
    16981 ctcctcgagc aagtagtcca aatggttggt agtcaataag cgaatcttat cctgctgctc
    17041 ataccaaaag tcaaaaaatt gccgtaaagc tagtaagaga tgggcatctg accaatccga
    17101 caagtcaatc tgccgctctg acaaccaggc ttgtaactcc atgttgaagg accgttcgag
    17161 cacctcttcc ttattggaaa agtttcggta aaaggtcacc cgaccgatgc cagctcgctc
    17221 taccaactca gatacactga tcttttcaaa agatttgtct tccagcaact cccacaaagc
    17281 ctccacgaca ctctccacaa ataacgattc tttccgcttc actctttcca tgcgaaacac
    17341 ttcctttcat tatgtttcct tttgggcaaa tagcagagct gggactagtt tttccactgt
    17401 ccatctgcta tactgataaa aaacgaaaca cttgtttcta aaaaaatacc ataaaggaca
    17461 gaaaaaagaa agtatgaaaa aagtttggaa aatagtagca gctgtaacag ttgttctagc
    17521 cttgcttggt tttattgcct acaaaaagac atttggctgg tcagctcctg agcttgttgc
    17581 tcagacccca caagtgaatg aatggtaccg cctcagtccc gaaggggttg tagattctca
    17641 gggcaatcag gcacacggac ttattcggac tggtaaagag aagaataagg tgatggtcta
    17701 tttcttcggt ggcggggtga gtattaatga agagactgct agtggtggca cacgttattt
    17761 tgcgaccaca actggccatc aagattatgt tgcgacttgg ggaattggaa gtccgcagga
    17821 agacaatcca tttaaggact ggactatgat tgtgcttccg tatgggacag gagattttca
    17881 tgcagggacc cagaactttt catatgtaga cgataacgga aaagagcaag tagtccatca
    17941 ccaaggttat tccaatctga tgagtatttt agcagcagcc aaaccgcacg ttggcaatcc
    18001 cgatacactt ctagtgactg gattttcagc tggcggattt gcgacctcac tgctagcgga
    18061 tgatgtcatt tctcattttc caacagcgaa aaatgtcaca gttgcagtgg acagttcctt
    18121 tttgagacat gataactgga aaagcattgc ggaaaatgtc tggaaaacac cgacttccat
    18181 tagtgatcgc ctgcattcag acaatattgt tttagatagc ttagtagccc tccatgagaa
    18241 acgcggggat agcgttaaga ttttatttga catttcctat cgagatggaa ttcttcagca
    18301 atatcaagcc tatattgacc aagggaaact agcagatgca acagaagaga gtagtgacca
    18361 ctttcaaaaa gaacttaaaa aaatgattca agaactgcaa tctaagattg atggcgtggg
    18421 tatttttatc tggaattatg ggcaagatga aaaaactacc gctactcagc acaccaccat
    18481 taacttctca acattcttta cccctatgtc taaagacaag agtgtggcag aatggctgga
    18541 tgatgctgtc aatggacagg taaacagtta tggattagaa ctattagaat aggagaacaa
    18601 catgaaaaaa tttaaaaaaa tcatcttatg gacccttggg attgtagcta gtctcggtat
    18661 cattgcttat gtgtctgtta ccaatcatcc gcagttggta gtcggtgtca ttcaatcgtt
    18721 tatgtacaag gagagttcgc cgaactctta tagtcctctt tatgatccca ttgatggttt
    18781 gaaagagaat ggtcagtatt taaaaagtga gattgcctat agcaaggact accctaacag
    18841 ctttttagat attacctatc cagaccaaaa tcttgaagct gaccgtccaa ccctcttcta
    18901 tttccacggt ggtggattct ttggaggcag caagaatatg ggggaccctc tggctgccag
    18961 ccaagcgacc tatctcattg acgatatcgt ttcaaaaggt tacaacgttg tcaatgttga
    19021 ctatgccctt gttccagact atcattttcc aacgcctgtg attcagatga atcaggccat
    19081 tgcctacatg aaggaacacg ctgaagaata ccatttgaac atggataatg tcgtcttgat
    19141 gggctcatca gcaggtgcca tcatggtagc acaatacggt agcctcttag ccaacaatga
    19201 ttacgctcag ctattgaaca ttgaaccaag tattgcaaaa gaacatgtca aagctctggt
    19261 tatcgacgac gctcccttag attacgccaa gtttagctta agcacgaagc tcttggttgg
    19321 aaattatgtt tcagaaacca tttatctaag tgaggaagaa atcaatgcct ataacccgat
    19381 ttcccatgtg aacaaggact acccagctag tttccttctg ggaagcgagt atcggacaga
    19441 tatggtgtcg ctcaataagg ccttgaagca agcgggagtt gaaaatgaat tggtcgatcc
    19501 tttagcagaa gaggggcttg aaaagcctca tggttttgtg gcgaatttac gaactgaccc
    19561 tgtgtctgcc aaagcatttg aacggttgat tacttttata gacgatagaa ccaaggagta
    19621 gtaggagagt ggataagctg gaaactgatg gggatttgtc caatcaatct gccgctctga
    19681 caaccatgtt tgtactcaga aagtctacgc cgaaaaattc caaatgagaa caataagtcc
    19741 cagctctgcc atttcccaaa agtacgttat aaaatcgttg aaatccttgt caattgtgtt
    19801 ataatgaaga gtatgcaata aaattttaag gagaatgaca gaatgtctgt atcatttgaa
    19861 gcaaaagaaa caaaccgcgg cgttttgact ttcacaatcg gtcaagatgc tatcaaacca
    19921 gaattggacc gcgttttcaa caaagttaag aaagatatca atcttccagg tttccgtaaa
    19981 ggtcacttgc cacgtgccgt tttcaaccaa aaatttggtg aagaagcact ttatcaagat
    20041 gttgtaaatg cattgttacc agcagcttat gaagcagcgg ttgcagaagc tggtcttgaa
    20101 gtagttgcac aaccaaaaat tgacgttgtg tctatggaaa aaggtcaaga ctggacaatc
    20161 actgcggaag ttgtgacaaa acctgaagta aaattgggtg actacaaaaa cttggcagtt
    20221 tcagtagaag ctactaaaga agtaacagat gaagaagttg atgcaaaaat cgaagctgca
    20281 cgcaacaact tggctgaatt ggtcatcaaa gaaggaccag cagctgaagg cgatacagtt
    20341 gttatcgact ttgtaggttc aatcgacggc gttgaatttg acggcggtaa aggtgagaac
    20401 ttctcacttg gacttggttc aggtcaattc atcccaggtt tcgaagcgca attggtaggt
    20461 cacgcagctg gtgaagaagt aaacgttgaa gtgactttcc cagaagacta ccaagcagct
    20521 gaccttgctg gtaaaccagc cctcttcgtg acaaaaatcc acgaagtaaa agcaaaagaa
    20581 gttccagctt tggatgacga attggcaaaa gacatcgacg aagaagtaga aacacttgat
    20641 gagttgaaag ctaaataccg caaagaattg gaagcagcaa aagaagttgc ctttgacgat
    20701 gcagttgaat cagcagctct tgaattggct gtagaaaacg ctgaaatcgt tgaattgcca
    20761 gaagaaatga ttcacgaaga agttcaccgt gcaatcaatg aattcttggg tggtatgcaa
    20821 caacaaggta tctcaccaga tatgtacttc caaatcactg gtacaactcg tgaagacctt
    20881 cataaacaat acgaagcaga tgctgaaaaa cgtactaaga caaacttggt tgttgaagca
    20941 gtggctaaag cagaaggttt cgaagcaact gaagaagaaa tcaacaaaga aatcgaagac
    21001 ttggcagcaa cttataatat ggaagtggca caagtacgta gcttgctttc accagaaatg
    21061 cttaaacacg acatcgctgt gaagaaagct gtagaagtaa ttacaagcac tgcaactgta
    21121 aaataattat ttggaaaact cgactctagt cgggtttttt gtttaatcat ctttcgatgt
    21181 tacaaagaaa ctctctgaaa aaccttgtcc gtcccatgca gaaatgggag aatgtcatat
    21241 ctgttggtgg agtcgtccaa gtttcacaag gggtacgaat aactgacgga gaggtcagtc
    21301 attaccagtt gccgtggtcg gtgctcctat gtttacaagc tatcgaatat gactgttctt
    21361 ttttagggag tagaatttta ttggactatt tgcaatccaa tctcatcata tcaggtgcct
    21421 ttgggttatt ttacaaggag tatgtcatag cagttggggg ttatgatagg cagacactgg
    21481 gagaagatat ggagctagtc atgaaattgc attatttctg taaaaataac aatattcctt
    21541 atcgaatctg ctatgaaacc agcgccattt gttggagtca agcaccgagc tctatcggtg
    21601 atttatgcaa acaacgccgt cgctggttct tgggactcta tcaatgtcta aaaaaatacc
    21661 gaaaaatgtt atcgcgagtt cgttttgggg cggtgggata gttttcctat gcttactata
    21721 tgctatttga atttttggct ccgtttatag agactttcgg ttgtttcgtg attctactgt
    21781 cgttgatcta tcaccaacta aacatctcat ttttcatatc gctctttttc ctctatagtg
    21841 ctttttgctc actaatcacc tttgtgtcat ttttgcaacg cgtctactcg caagatttat
    21901 ttattggacc tatggatttg tgtaaagcct tcctggcaac cttgtttaga tattttttct
    21961 tgcattgggt attgaatatt gtccgtctga caagttttat tggatataaa aagagaaagc
    22021 gtgaatgggg agaaatcaaa cgagtgaaac aattcgttta agtaaaggta agactggctt
    22081 ataaaaagaa agtcttatct ttttttaaaa aaatacttga ctggttaata ttcttgtgat
    22141 atagttaact ggttaacaaa ggaggtagtt atgttaaaga aagtgataag aggctgtttt
    22201 gttgctttat tcggttttgt tttagcagct tgctcggctc agaaggaagc gagtcaagtg
    22261 cagccaggaa tgaaaatagt cacctcattt tacccgattt attcactggt taaggaagtg
    22321 tcagggaata aaaatgatgt tcgaatgatt ggctcaagac agggtataca ctcttatgaa
    22381 ccatcggctg cggacataaa ggccatatac gatgcagatg tttttatcta tcattcgcga
    22441 attttggaat cctgggcagg acgtttggaa cctaatttgc aaggttcatc tgtcaaggtt
    22501 ttggaggctt cgacaaattt accgctgaca aaggttccag ggttagaaga catggaagct
    22561 ggtcaaggga ttgatgaagc tagtttatat gacccccaca catggctgga tccagttttg
    22621 gttggtcagg aagctgtttc gattggtgaa cttttagcag aaagtgatcc taagaatgcg
    22681 gattattatc gtcaaaatgc cgcaacttta gaggaaaagg cgcaaaagtt ggcagacaag
    22741 tatagcccaa tctttttaaa agccacatca aaaacatttg tcactcaaca tacagccttc
    22801 tcttacactg cacaacgctt cgggttgaag cagttgggaa tcgcaggagt ttcagaggaa
    22861 gagccaagtc ctagacagtt ggcggaaatt aaagaatttg ttgacaccta caatgtgcaa
    22921 acaattttta cagaaaaagg tgcctcggat aagcttgcca aggcattggc gagttcgaca
    22981 ggtgtagatt taaaagtgtt ggatcctctt gaagcagatc cagaaaataa tttaacctat
    23041 ctagaaaatc tagagcaagt cttagaaaca ttagctcaag agttaaaata aaataccgaa
    23101 ttctatcgac cgaaaggaga aaaaatgaag aaaaaagcag ttgttggctc cgtagccgcg
    23161 cttgttttag ggatgagtct ctgtagctat cagcttggac gtttccaagc gatggaagag
    23221 caaaaaaatc gtgtgtccta tattgaagat agtcaaagtg tacaaactac cgttgctgaa
    23281 caattaacac ctgatcaagt ttctgccaag gaaaatattg acgctgagca gatagtcgtt
    23341 aaaattactg atcaaggcta tgtgacatca cacggtgacc atttccacta ctataatggt
    23401 aaagtccctt ttgatgcgat ttttagtgaa gagttggtca tgaaggaccc aaactatgtc
    23461 ttacaagaca gtcacatcat caatgaagtc caggatggct acgtcataaa agttgatggg
    23521 aaatactatc tctatctaaa agaccctagc aaacataaaa atgttcgtag caaagaagaa
    23581 gtggagcggc aaaaaggaat ctcttctgct gacagcaaaa atcaggcagc aggaaatagt
    23641 aaagacggcc gctacagaac agacgatggc tatgtcttta atccgacaga tgttattgaa
    23701 gacacgggag atggttttat cgttccgcac ggcgaccatt ttcactttat tcctaaaaaa
    23761 gatttatcag ctaccgaatt gaaggctgct caagattact ggaatcaaaa aggaagtgtg
    23821 agctcggcta gtggtagtca atatggcgat agaaataata gagctcaaca gacaacaatt
    23881 agtgcggggc aaggtcagga tttggctagt ttattggctc aattggatgc aacaccccta
    23941 tctcaacgcc atgtagaagc agatggattg gtatttgacc caagaacgat tacgaagaaa
    24001 accgcagcag gtgtcatcgt tccacacggc gatcattacc actttattcc atacagccag
    24061 atgtctcctc tagaagagaa gatttctcga atgattggtg taaatggagc aggtgtttct
    24121 tctggtgcac aagcaagtca ctcccaacat acaccaacac aaccaaatcg tccagtaaca
    24181 ccaatcggca cggttacaac gcaaccggta tcgcctacac aaccagtatt accaacacaa
    24241 ccgaagcagt caacagggaa agtggtttcc tatatgggac gtcaaattcc tgcttacgga
    24301 aaagggttag atggtaaggc atattttaca agtgatggat acacctttag caaagactct
    24361 attacctcag ttgatgatca agggctgata gctagtcatg gagatcattt ccattatgtt
    24421 ggttttggcg aactcgaaga ttttgaaatc aagcaagtag aggaatgggt taatgagaag
    24481 gcgggcaaac aagtgccgcc gaaaacatct gaacaagtag gaaacgatgc taaacccaca
    24541 aaaccaagtc aagggaatga ttcaaaacct gtaaaaccaa ttcaagaaga aaatcgccct
    24601 gcatttgaat acaaacaagt aacggctaaa cgtaaattgg ctggaaaagt tgtttatgaa
    24661 atggaagtag gtggaaaaac ttatacatat ggtcgtgacg aactagattt gatgaaaatc
    24721 tcttttgctg agttgacttt ggcagaaaaa gataagcagt atatctttga tattgcaccg
    24781 cttgcagaag gagatcttaa gccagccatg ttggtcggta tggatcaaat tccgatgaag
    24841 ggagcgaatg caacctatga tactggacaa tcctttatta ttccacacat tgatcacatc
    24901 cacgtcctac cttacacatg gttgagtaag gaacaaattg caacgatccg ctatatcatg
    24961 caacatcctg aaattcgtcc atctgcttgg actacaagtg gacatggtga tggagaagca
    25021 acggatcttg ttcccccaat tttaaatgca acacctaaag ccaaccgctt aggcttgaaa
    25081 aattggcaaa tcattcacac agcagaagaa gtcatggatg cacgtgcaaa agggaaattt
    25141 gcgacaaatg atggctatat cttctcggcg gaagacgtac tagatccagc aagttttgtc
    25201 tttagtcaag cgttcagtct accaagagcg acaggtggtt ctctgcgttc aatttccaag
    25261 aaagatttat ctaaagaaga attggaagct gtacaaactc ttcttgataa acgagacgct
    25321 gaagaattgg cgcaaaatgt cacaccgatt gagaaacgtg caggtttgaa aaattggcaa
    25381 attgttcact cggcagaaga gttggcagaa gctaaggcag caggaaaata tacaacgaaa
    25441 gatggctata tttttgaccc agcagatcta ttagatccca aagtgaaaat aggtacggat
    25501 aactatcgca tcccacgtgt gattacagat ggttatcgtc ggattaacaa gagtgatttg
    25561 aattacttaa gtgagctaat cccagcagag gctatggtgg ctcaacgtga gaaatctaat
    25621 tctagttcac cgtcaacacc tgcaccaact gaaacaggag caagtgcagg cgagacaaca
    25681 aagccagaac agccacaagt tgccaaagaa acagcagagg aaatttataa ccgagtggaa
    25741 gcgaagaaag tcgttccttt cgaagcactg acctacaatg ctggctatgc gaccgaagta
    25801 cgtaacggga ctctggtaat tccgcatcaa gatcactatc actatgtatc ctttaaatgg
    25861 tttgaccaag gtagtgcaag aagtcctgaa gggtatagtt tagaagattt cctagcaacg
    25921 gttaagtact acatgactaa tccacaagaa cgccctgttt cagacgatgg ctggggagta
    25981 tttacaccaa atacaccatc tgaatcaaca gaagaaacag agacggaaga aagtgatgaa
    26041 gaaattattt ctgaggaaac cgaggaaatt gacgagttta cagaagaatt aaaacgaaga
    26101 gcagaagagt ttggcatgga cttcaaaacc tttgaacaaa gtctggtcac actctcagac
    26161 cgttacaaag tttcttttga agctttcgaa tatgatgcag caagcaaagt tgttcgactt
    26221 gtagataagg atggggtcaa acggaccatc tcccttccaa gtttagaaga acaagtatag
    26281 taaacaaaat acctgtagcg ctg
//
LOCUS       DASFSW010000011        25072 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.011, whole genome shotgun sequence.
ACCESSION   DASFSW010000011 DASFSW010000000
VERSION     DASFSW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25072
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.011"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            22..822
                     /locus_tag="U1099_000395"
     CDS             22..822
                     /locus_tag="U1099_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM4866743.1"
                     /translation="MKKILALAATVLAGLTLAACSSTSSQSALDKIKEKGTLVVATSP
                     DYAPFEFQALVDGKNEVVGADIMLAQKIADELGVKLEVSAMSFDNVLSSVQNGKADIA
                     IAGLSYSEERAKVFDFSESYYQISDVLLIKKDSANSLTSIDAMSGKTLAVQKGSTQES
                     YAQENISQANLISLTLMGEAVNELKSGKVDAILMDSPVAAGYVSQNSDLAVASVEFPT
                     IDENSKVIALPKGSTELKTAIDKVIAEVKASDEFDTFLEKAATYASVE"
     gene            complement(950..2788)
                     /gene="glgB"
                     /locus_tag="U1099_000396"
     CDS             complement(950..2788)
                     /gene="glgB"
                     /locus_tag="U1099_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEM4866744.1"
                     /translation="MTEFTDLDLYYMNAGEHTTLYEKMGAHIIKERNKIIGTQFRVYA
                     PNAREVFLVGNFNEWQRSHSMEREIDGVFQLYVDGLKSLEDYKYLIITHDGREIYKAD
                     PYAFFSQVRPDTASTTYNSRYKFKDDLWMYNRVNYDFKEQPVSIYEVHLGSWKQKFAN
                     KNEGGAPVEAFNSYKEITPLLIEHIKENNYTHIELLPITEHPLDASWGYQVTGYYSPT
                     SRYGKPDELKYLVDQCHQAGIGVILDWVPLHFCKDAHGLYQFDGSWLYEYSYEQDREN
                     HQWGTANFDLGKGLTRSFLLSNLKYWLEYFHFDGIRVDALSYLLYWRGETDEDKINHA
                     AIDFIKRVNAMVHTDYKGVLMIAEDSSSFPKVTHSLEDGGIGFDMKWDLGWMNDTLKF
                     VERPAIYRKYHSNEITFGMYYNQNEQFLLPLSHDEVVHGKHSIIEKMNGDYEDKFHLA
                     RVYYSFYFAHPGKKLLFMGNEWGHIREWHEYTEMDWGLLQFPIHHSFYQMMKTLSTFY
                     KEHDAFWKYDYQAYEKGFNWVKIDEEASLYAFSRTSDVQEILTIHNFNDQELFDVHLD
                     LPADSEYKLVFSSNAIPMDTFSLYPDENGARITVPRLTSFYLERVK"
     gene            complement(2861..4306)
                     /gene="glgA"
                     /locus_tag="U1099_000397"
     CDS             complement(2861..4306)
                     /gene="glgA"
                     /locus_tag="U1099_000397"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEM4866745.1"
                     /translation="MTKKSVLFVASEGLPFIKTGGLADVIGSLPKELVKQGLDVRVVL
                     PLYKKIAVNNHSDFDYVSSFDVRAGDIQSMANVYSQVVDGVTFYFIEHRDFFERDELY
                     GYDDDAMRFGYFQHATCRLLEALHFFPDVMHTHDWHTAALPFLCRTFYSYREEFRNIK
                     HVFTIHNLAFQGIFHKQALWSALGMDYSYYLDGIARFHDECISFMKLGILYADKVTTV
                     SETYAREILTEEFGENMQHVLELRKYDLVGIVNGIDYDSWDSQTDQYLVRNYSLETIE
                     SKKANKLALQAQFALPQDENVLLIGIVSRLTWQKGFYLLTEVLGHLLQAHVQFVILGN
                     GEEDIENAFNHFKHAYPDKFAFYRGYNEPLAHQIYAASDLFLMPSMFEPCGISQLISM
                     HYGTLPLVRETGGLVDTVAPYNMSTKEGTGFSFGGRDAYNMRQVYDLALQTYYDRPDD
                     WYAMVEQAMKRDFSWTASAEKYIWLYREISG"
     gene            complement(4315..5430)
                     /gene="glgD"
                     /locus_tag="U1099_000398"
     CDS             complement(4315..5430)
                     /gene="glgD"
                     /locus_tag="U1099_000398"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEM4866746.1"
                     /translation="MLRNTLGIVNIEGNNVHFGDVMKHRGVQAFGFLGRYRLIDFALS
                     NMSNSGITEFQVYMPAEMRSTIQHVGTGKHYNINSKRGTLRLLNSATDPNSVYRHDIN
                     AFTENIHYIENSTKDYVLVAPSYFIYSQDFSKVMDDHIATDADITVLYKNVSDAKENF
                     IGCQTLKFGEDHRVVAFEENHGKYKNRPVSLEAYIMKRTTFIDLIKRANKVSSLYWLK
                     DILRDTVDQYKIMGYAHRDYVACINTTASYFNTQIELIDQDTRKLLFKHDWPIHTQTS
                     DSSPCLYGPLAEVKRSLISNGANINGQVENSVIDRDVIIEEGVVIKNSIILNGVTIKS
                     GANIENAIIDKATKIIHPIDIKGSETSPSYLKPHQII"
     gene            complement(5443..6570)
                     /locus_tag="U1099_000399"
     CDS             complement(5443..6570)
                     /locus_tag="U1099_000399"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEM4866747.1"
                     /translation="MAQNKMLAMILAGGRGTRLEGLTKKVAKPAVAFGGKYRIIDFPL
                     SNCANSGIDIVGVLTQYEPVLLNSYVAQSQRWGLDVQGSGVFVLPPSEKIEGFGLYKG
                     TADAITQNIDFIDLHDPEYVLILSGDHIYKMNYDKLLDTHIQKKADATIAVIEVPIKE
                     ASRFGIMNTDEDYRIEEFEEKPENPKSNLASMGIYIFTWKTLKKYLQEDDKLDTSSHD
                     FGHDIIPKYLEDGRTLIAHPFRGYWKDVGTVNSLWESNMDLIDHAGDLDLSDRSWRIY
                     SEDKGSPAQVIGATATVKSAYIDKGAIIDGTVEHSVISTDVQVNQNAVVKNSVILPGA
                     IIGEGVELDYVIVAENIKIADGVKLTGDIDHILLIDKNVTK"
     gene            complement(6734..7645)
                     /locus_tag="U1099_000400"
     CDS             complement(6734..7645)
                     /locus_tag="U1099_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM4866748.1"
                     /translation="MRIQQLYYIIKIAETGSMNEAAKQLFITQPSLSNAVRDLEKEMN
                     IKIFLRNSKGITLTKDGMEFLSYARQIVEQTELLEVRYKNPNAKRQLFSVSSQHYAFV
                     VNAFVSLLKETEMEDYELFLRETRTWEIIDDVKNFRSEIGVLFLNHFNRDVLLKLLDD
                     NRLSYTTLFTAKPHVFVSKTNPLAQRKSITLDDLVDFPYLSYEQGIHNSFYFAEEILA
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLEFDDEIELVYI
                     QHEKALLSDMGEKFINYLLEEVKFDQK"
     gene            7809..8633
                     /locus_tag="U1099_000401"
     CDS             7809..8633
                     /locus_tag="U1099_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEM4866749.1"
                     /translation="MSTFLFSVSLLYRIGEKMILKEQMLLVDQVQLAPRIFAMTLQGD
                     MVQDMKMGQFIHIRVPDDSMLLRRPISISEIDRNKSQCRIIYRVEGQGTEVFSKMTVG
                     QYLDVMGPLGKGFEVDFLKEGDRILIIGGGIGVPPLVEVAKRAAFRGVNVTSVIGFAT
                     KEAVILEEELASYGMVYVTTDDGSYGRKGNVATVVEELTNEFAAIYSCGAPAMMNYVD
                     QRFQEHPHAYISLEARMACGMGACYACVVKPKEGQEHENKRVCKEGPVFATGSLIL"
     gene            8643..9584
                     /locus_tag="U1099_000402"
     CDS             8643..9584
                     /locus_tag="U1099_000402"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEM4866750.1"
                     /translation="MIKRLAISLPGLDLKNPIIPASGCFGFGQEYADYYDLNQLGSIM
                     IKATTRHPRYGNATPRVAETPAGMLNAIGLQNPGVDVVLSEKLPWLEQHFPDLPIIAN
                     VAGFSNEEYAYVSGKISKAPNVKAIELNISCPNVDHGNNGLLIGQVPELAYQAVKAAV
                     EASSVPVYVKLTPSVADITLLAKAAEDAGATGLTMINTLVGMRFNLKTRQPILANGTG
                     GMSGPAVFPVALKLIRQVAQVTDLPIIGMGGVDTADKAIEMMVAGASAVGVGTANFTD
                     PFACPKIIQDLPKRMEVYGIDSLEGLRREIRHRLGKR"
     gene            9749..10444
                     /gene="pyrF"
                     /locus_tag="U1099_000403"
     CDS             9749..10444
                     /gene="pyrF"
                     /locus_tag="U1099_000403"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEM4866751.1"
                     /translation="MKETRPIIALDFAGKEEVQSFLEQFPPDEKLYVKVGMELYYAEG
                     PGIINYLKGCGHSIFLDLKLHDIPNTVESAMKVLAKLGVDMTNVHAAGGVEMMRAARR
                     GLGKDAVLIAVTQLTSTSEEQMRTDQNIQTSLQEAVVHYAQRAQEAGLDGVVCSAHEV
                     ALIKDATSSDFVCLTPGIRPTGGEVGDQKRVMTPADAARIGSDYIVVGRPITKSEHPY
                     QTYLSIKEEWNRG"
     gene            10437..10763
                     /locus_tag="U1099_000404"
     CDS             10437..10763
                     /locus_tag="U1099_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEM4866752.1"
                     /translation="MDKIYQVIIDRPIGYIDKFGNQYPINYGYVPNLLAGDGEEQDVY
                     IISKAVRQPLTAFEGKLVAIIHRRDDVEDKWVLTGLDEKLTVTEIKEKTNFIEQYFDS
                     WIEMVD"
     gene            10900..11532
                     /locus_tag="U1099_000405"
     CDS             10900..11532
                     /locus_tag="U1099_000405"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEM4866753.1"
                     /translation="MTLATEIASHLLDIKAVHLRPEKPFTWASGIKSPIYTDNRITLS
                     YPETRNLIEDGFVQRIEEEFPEVEVIAGTATAGIPHGAIIADRMNLPFAYIRSKPKDH
                     GAGNQLEGRIVKGQKMVVVEDLISTGGSVLDAVAAAEREGADVIGVVAIFTYELPKAD
                     CNFDNAGVKLVTLSNYSELIKVAKVQGYINADGLNLLKKFRQNQETWQED"
     gene            11641..12567
                     /locus_tag="U1099_000406"
     CDS             11641..12567
                     /locus_tag="U1099_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4866754.1"
                     /translation="MISVIVPCFNEEEAIPYFYDAMEKVRKEMGEQFEYIFVNDGSKD
                     RTLTVLRQLSGQDRAVRYLSFSRNFGKEAALYAGLQAAQGELVTVMDVDLQDPPEMLM
                     EMKAMLDGNPDLDCVGTRRVSRDGEPPIRSFFAKLFYKLMNKISQVEVVDGARDFRLM
                     RRHMVDAILSVSEYNRFSKVIFAWVGFETEYLPYENVERVAGETSWSFWKLLSYSIEG
                     IINFSDTPLNIASYTGFFTFLLSLVLMVFVVFKTLVFGDPTIGWPSTICIILFLGGLQ
                     LMTIGILGKYLAKVFLETKKRPIYIVKEKSSN"
     gene            12577..13230
                     /locus_tag="U1099_000407"
     CDS             12577..13230
                     /locus_tag="U1099_000407"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEM4866755.1"
                     /translation="MEHSAWHELIKAQLPEHYFGKINQFLDYVYAGGTVYPPREKVFA
                     AIQTTDLEEVKVVILGQDPYHGPGQAQGLSFSVPNSVPAPPSLQNILKELTDDIGVKQ
                     DHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIKVVNSLDQPVVYILWG
                     AYARKKKALITNPKHLVIESAHPSPLSAYRGFFGGKPFSQANAYLTSQGRTGIDWLR"
     gene            13270..14541
                     /locus_tag="U1099_000408"
     CDS             13270..14541
                     /locus_tag="U1099_000408"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEM4866756.1"
                     /translation="MLLVKNGRVLDPQSGLDKVCDILMDGSEIVKIAESISEDVEQEV
                     IDATGLVVAPGLVDVHVHFREPGQTHKEDIHTGALSAAAGGFTSVVMMANTNPTISNV
                     ETLKEVLDSAKKEAIHIYSVATITDHFDGQNLTDFPALLEAGAVAFSDDGIPLTNAGV
                     VRKAMKLAKEQGCLISLHEEDPDLNGVLGLNEQVAEKHFHVCGATGVAEYSMVARDVM
                     IAYETGARVHIQHLSKAESVKVVEFVQGLGANVTAEVAPQHFSRTEDLLLIKGANAKM
                     NPPLRLESDRLAVIDGLKSGVISVIATDHAPHHADEKNVEDLTQAPSGMTGLETSLSL
                     GLTHLVEDGHLTLLQLLEKMTINPAKLYGLDAGYLAENGPADLVIFDPNANRLVTADF
                     ASKSANSPFVGDSLKGQVLYTIANGQIVFGK"
     gene            14672..16786
                     /locus_tag="U1099_000409"
     CDS             14672..16786
                     /locus_tag="U1099_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEM4866757.1"
                     /translation="MKKKKILLPVMSTLLLAPFVLAQQVQAAETTTAATTTNQPATTD
                     ATATVPATSVENVATEETVVPAAEETVEAVIIHTNDVHGRILEEKNVIGDAKAAAVIE
                     EERAKVENTIVVDAGDAFQGLPISNSTKGEDRANIMNQVGYDAMAVGNHEFDFGMDQA
                     IKYKETLNFPLLSANTYVNGARVFEASTIVDKTPTVVGDEFVVIGVTTPETATKTHPK
                     NVEGVTFTDPVTEVNKVIDEVEARALADNRVYKNYIILAHLGVDSTTPVEWRGSTLAE
                     ALSKNSKLAGKRVIVIDGHSHTVEATTYGDNVTYNQTGSYLNNIGKVTLKSDKLLGEA
                     SLISAADTKNVTPNAKIAALVDEIKAKYEAENAQVVIENNPVELNGDRSNVRVRETNL
                     GNAVTDAIYAYGQTGFSNKTSLAVTNGGGLRATIAKDQPVTKGDIIAVLPFGNIVSQI
                     TVTGQQIYDMFTKSLSSTLQVNPETGEMLLDENGMPLFEASGGFLHISGANVFYDPTL
                     PVEERVLLIGILNPETGEYDALDLEKTYYLATNDFLAAGGDGYTMLGGAREEGPSMDS
                     VFAEYLKTADLSAYEVVNPYSRIIPVNSSIDTDEDGYPDFIEILLDTDPENPASNPET
                     VPAENTDSPSNQVQNTSATDKKAPVDSPKVGDKKTEVASPVKTTKAGVLPNTGDQMNL
                     TLSLFGLGLAGLAMAVGRRKEN"
     gene            16838..17485
                     /locus_tag="U1099_000410"
     CDS             16838..17485
                     /locus_tag="U1099_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEM4866758.1"
                     /translation="MQTVFQYQKEGDREQISSYVLLSNSPRRKQLLGFLQPQIASVEV
                     DERGIEEHFMKQFATDDFLTRAAKTCCEISKAKSDMDLVTGHLYISADTIVVSDGQIF
                     NKPQDLTEAEIMLRSYFGKSHHVVTSVCLRTRHSLDVFYTVAQIDFVDYYPALEEVIQ
                     SYIREKQPLDKAGAYGIQELDPRFVSGISGDIHTIIGLPVAEVSRRIFSEEGFEK"
     gene            17537..18025
                     /locus_tag="U1099_000411"
     CDS             17537..18025
                     /locus_tag="U1099_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEM4866759.1"
                     /translation="MTVDIERYKQLAQQKQGEHRKFLANLKKKAPKNLDRIVQDIHAE
                     VFSEIDCTECVNCCKTLGPLFTEADITRIAKHLRMKLPVFEDMYLRVDEDGDKVFQSM
                     PCPFLGGDNLCSIYDARPKACREFPHTDRKKIYQINNLTIKNTLICPAAYLFVEKLRE
                     RI"
     gene            18125..18976
                     /gene="ylqF"
                     /locus_tag="U1099_000412"
     CDS             18125..18976
                     /gene="ylqF"
                     /locus_tag="U1099_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEM4866760.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKVDLADPVRTKEWQSYFEGQGIRTLAINSKEQATVKKVTDAAK
                     SLMKDKIERLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKDLEILDTPGILWPKFEDQEVGLKLALTGAIKDQLLPMDEVTIFGLDYFKANY
                     PERLQERFKGMDLEQETPELIMDWTKKLGFRDDYDRFYNLFVKDVRDGRLGTYTLDRV
                     SDLDGND"
     gene            18963..19742
                     /locus_tag="U1099_000413"
     CDS             18963..19742
                     /locus_tag="U1099_000413"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEM4866761.1"
                     /translation="MATIKEVTTLLAQVDSLDSPVWAELSQDERAGVQAAIKKRRKEL
                     DKEVAEDARLEAMLFYEKNLYENGVEFIAGIDEVGRGPLAGPVVAAAVILPKGCKIRY
                     LNDSKKIPKSKHEAIYQEVMERAVAVGVGIKDAAVIDQVNIYEATKLAMLEAFGKLSQ
                     KPDHLLIDAMKLDTPIPQTSIIKGDANSLSIAAASIVAKVTRDKMMADYDKEFSGYGF
                     AKNAGYGTAEHLEGLNKLGITPIHRKTFEPIKSMVTGGKSS"
     gene            19739..20749
                     /locus_tag="U1099_000414"
     CDS             19739..20749
                     /locus_tag="U1099_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEM4866762.1"
                     /translation="MTVFFWIFLASIGLFGLLYFWERRSIFISLFALNILVWALAWII
                     STGILENNEILRLLAIAVAMVLFLAFLSGPFVLLFTLYLNGFQILEREGVRFHNFLSM
                     GLAVALTFYLFIAPFVVQSLSGISLLNMVFVYVGFLVSYAIIISMLYTTSSFVNFVNL
                     FPGKLDYVVVLGAGLIGDKVTPLLASRIDKGIAIYQKQPGSKLIMSGGQGPDELIAEG
                     QAMANYALEQGIPAEDIIIENQSTNTEENLKFSYALMKPGSRFALVTNYYHVFRALLL
                     ARQLKIKCIGYGAKTKFYFSLNAFIREFVGYVVMSRKTHLVFLSIVSALYLIGVIISL
                     MH"
     gene            complement(20782..21432)
                     /locus_tag="U1099_000415"
     CDS             complement(20782..21432)
                     /locus_tag="U1099_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEM4866763.1"
                     /translation="MNPSLKLSLQLSFGEEVANSVTHAVGALLMLVLLPVTAIHSYQD
                     YNLTAVIGMSIFVISLFLMFLSSSIYHSMPYASTHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVGGWLGYLIVGLQWGLTIFGILYKIFAKKINEKFSLFLYLFMGWLVVFILPAIL
                     QKTGMAFGILMLAGGLSYTIGALFYAKKKPYFHMIWHLFILLASALQYIAIVYFML"
     gene            complement(21422..21973)
                     /locus_tag="U1099_000416"
     CDS             complement(21422..21973)
                     /locus_tag="U1099_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEM4866764.1"
                     /translation="MKQTIPYWHELPNLDLYLDQVLLYVNQVVNSQESLEQKSLYSMK
                     INSSLGNETDDRTGVHQGKLTTDNVDFRRVLTAAMINNYVKHKQIEKPVKKKYQKQQV
                     ARLIALTILKNVFSIQEISQTLHLLLQSSHSETLYNHFVDCMRNEENEETPDIIRYAC
                     QSVKLYYKTRQLTMELERSHHES"
     gene            complement(22081..23733)
                     /locus_tag="U1099_000417"
     CDS             complement(22081..23733)
                     /locus_tag="U1099_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM4866765.1"
                     /translation="MKIIIVGGVAGGMSAATRLRRLMEDAEIIVFEKGPYVSFANCGL
                     PYYISGEIAEHDSLLLQTPESLKQRFNLDVRPFHEVISIDAEQQTVTVKTPTGELIES
                     YDKLILSPGAKPVIPNLSGIEEANNLFTLRNVPDLDKIMIKLDQIKSGHVVVIGAGFI
                     GLEMAENLALKGWKVTLVEQAPHVLPTFDLEMARYVEAELTNKGIEVITGQSVTAFHE
                     KGQVLELSNGQTLSSDVTILSVGVAPDSQLAKKTGIELGLRGGILVDKNYQTSQNNIY
                     AVGDAIVVKQEITGQDALISLASPANRQGRQVADVIAGLDRKNKGSIGTAIVKVFDLA
                     AASTGLSERTVQSLGYEHAIIHTTGKDHAGYYPGATDIVLKLIFSPTDGKIFGAQAVG
                     QKGIDKRIDVLATAIKAGLTIFDLPELELTYAPPFGSAKDPVNMIGYAAMNITEGISQ
                     NIQWHQLDDELATGKVLLDVRNPGEVANGQFREFIHIPLDQLRERIGELDASKEYIVI
                     CHSGLRSYIAERILKEKGFGVKNLDGAFSLYKMALPENIIQP"
     gene            complement(23730..24053)
                     /locus_tag="U1099_000418"
     CDS             complement(23730..24053)
                     /locus_tag="U1099_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM4866766.1"
                     /translation="MFKQLFSGMFSNSDSLPITDVEQAIQSSNAILLDVREVDEFASG
                     HIPQAINQPLSQLDQFKGDKTKNYLVICQSGMRSQRATEYLKSQGYQAINIKGGMNAW
                     TGGIA"
     gene            24172..24432
                     /locus_tag="U1099_000419"
     CDS             24172..24432
                     /locus_tag="U1099_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEM4866767.1"
                     /translation="MNTDKKKMINRLKRAEGQLRGIQKMIEEDQECIDIVTQLSAVRS
                     SINSMMGMVIAQKVQDCITHPSENPEEQEARLAQAINLIIKK"
     gene            24443..25000
                     /locus_tag="U1099_000420"
     CDS             24443..25000
                     /locus_tag="U1099_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEM4866768.1"
                     /translation="MPTEKEKMIAGQFYMAADAELREMRTFARQQMKKFNEELDGQAR
                     SERLKNWLGATGERIYVEPNFYCDYGSNIHVGEDFYANFNCTMLDVCPITIGKNAMLG
                     PNVQLLTPLHPLDPDERNSGLEYGSPITIGDNFWAGGGVTILPGVTLGNNVVVGAGSV
                     VTKSFGDNVVLAGNPARVIKEIPKK"
BASE COUNT         7323 a         4803 c         5618 g         7328 t
ORIGIN      
        1 aagagaaggt atttaggaaa tatgaaaaaa atcttggcat tagcagcaac agttttagct
       61 ggactgacat tggcagcttg ttcttcgacg tcatcacaat ctgcattaga taaaattaaa
      121 gaaaaaggaa cattagtagt ggcaactagt ccagactatg ctccattcga gtttcaggca
      181 ctggttgatg gaaagaatga agttgttggt gcagatatta tgttggctca aaaaatagcc
      241 gacgaacttg gggtcaagct tgaagtttca gcaatgagtt ttgacaatgt attgtctagc
      301 gttcaaaatg gcaaagctga tattgcaatt gctggtctat cttattcgga agagcgtgca
      361 aaagtttttg atttctctga aagttactat caaatttctg atgtattact tatcaaaaaa
      421 gatagcgcca attcactcac ttcaattgat gctatgagcg ggaaaactct cgcggtacaa
      481 aagggttcaa cacaagaatc ctatgcacag gaaaacatta gtcaagctaa cctaatttct
      541 ttaaccttga tgggtgaagc tgtaaatgag ctcaaatcag gaaaagttga tgctatttta
      601 atggattctc cagttgctgc tggttatgtt tcacagaatt cagatttagc cgttgcatcc
      661 gttgaatttc caacgattga tgagaattcg aaagtcattg ctttgccaaa aggtagtaca
      721 gaactaaaaa ctgcgataga caaggttatc gcagaagtaa aagcaagtga tgagtttgat
      781 actttccttg agaaagctgc aacatatgca agtgttgaat aagagaaatg aacaatcatc
      841 ggaaacgatg gttgtttttg catgcaaaaa agaatggagt aagtgagcca caccccaaaa
      901 gttagacaga aaatttgggt cggttcaata ctccattttt tatagataat tatttcacac
      961 gctctagata aaaactggtc aaacgtggta cagtaattct ggctccattt tcatcgggat
     1021 agaggctaaa tgtgtccatt ggaatagcat tagatgagaa gacaagttta tattcgctgt
     1081 cagctggcaa atccaagtgt acatcaaata gttcttgatc attaaagtta tgaatagtca
     1141 atatttcttg cacatcacta gtgcgagaaa aagcatacag gcttgcttct tcatctattt
     1201 ttacccaatt aaatcctttt tcataggctt ggtagtcata tttccagaaa gcatcatgtt
     1261 ctttatagaa agtagatagt gttttcatca tttgatagaa agaatggtgg attggaaatt
     1321 gtagcagtcc ccagtccatt tctgtatatt cgtgccattc acggatgtga ccccattcat
     1381 ttcccatgaa gagtaatttc tttccaggat gggcaaagta aaagctgtag tagactcgag
     1441 ccagatggaa cttatcttcg tagtcgccat tcatcttctc gataatggag tgcttcccat
     1501 gaacaacttc gtcgtgcgaa agtggtagta agaattgctc gttttggtta tagtacatac
     1561 caaaagtgat ttcatttgag tgatattttc gataaatagc agggcgttcc acgaatttta
     1621 gtgtatcatt catccagccc agatcccatt tcatatcaaa tccaataccg ccatcttcta
     1681 gagaatgtgt gactttaggg aagcttgaag agtcttcagc aatcatcagg actcccttat
     1741 agtcggtatg gaccatggca ttgacacgtt tgataaagtc aattgcggca tgatttatct
     1801 tatcctcatc tgtttcacca cgccaataga ggagataaga tagtgcatca acacggatgc
     1861 catcaaaatg gaaatattcc aaccagtatt ttaaattaga aagaaggaaa gaacgagtca
     1921 gtccttttcc aaggtcaaaa ttagcagtcc cccactggtg gttttcacgg tcttgttcgt
     1981 aggagtattc gtatagccaa ctaccatcaa actggtagag cccatgagca tctttacaga
     2041 aatgaagtgg tacccagtca agaatcactc cgattcctgc ttggtgacat tggtcaacta
     2101 aatacttaag ttcatctggc ttgccatagc gactagttgg actgtagtag cctgttacct
     2161 gatagcccca agacgcatcg agaggatgct cggtaattgg caataactca atatgtgtgt
     2221 agttattttc cttgatatgt tcgatgagaa gtggagtaat ttccttataa ctattgaaag
     2281 cttctacagg agctcctcct tcatttttat tagcaaattt ttgcttccac gaacctaagt
     2341 gcacctcgta gatggatact ggctgttctt taaaatcata gttaacacga ttatacatcc
     2401 agaggtcatc tttgaattta taacgcgaat tgtaggttgt agaggcagtg tctggacgaa
     2461 cttggctgaa aaatgcataa gggtcagcct tatatatttc acgcccatcg tgtgtgataa
     2521 tcaggtattt ataatcttca agggatttta atccgtctac ataaagttga aatactccat
     2581 caatttcacg ctccatagag tgactgcgtt gccactcgtt aaagttgcca actaagaata
     2641 cttctctggc atttggggca tagacacgaa attgggttcc gataattttg tttcgttctt
     2701 ttataatatg agcacccatt ttttcgtaaa gagtggtatg ctcaccagca ttcatataat
     2761 ataaatctag gtcagtaaat tctgtcatag caatctccat tgccgaaaag gagtggacta
     2821 gggtcccact ccgattcagt atattttttc atcttatttc ctaaccacta atttcacggt
     2881 agagccagat gtatttctct gccgaagctg tccaactaaa atcacgcttc atagcttgtt
     2941 caaccatggc ataccagtca tcgggacggt catagtaggt ttgaagggct aaatcataga
     3001 cttgtcgcat attgtaagcg tcacgccctc caaagctaaa accagttcct tccttggtag
     3061 acatattgta tggtgctaca gtatcaacca agccacctgt ttcacgcact agagggagtg
     3121 ttccgtagtg cattgaaatc aactggctaa tgccgcatgg ttcaaacata gaaggcatga
     3181 ggaagagatc gcttgctgca taaatttggt gggcaagtgg ttcattgtaa ccacggtaga
     3241 atgcaaattt atcaggataa gcgtgtttaa agtgattaaa ggcattttca atgtcttcct
     3301 caccattgcc taagataaca aattggacat gggcttggag aagatggcca agtacctcgg
     3361 tcaagaggta gaaccctttt tgccatgtca aacgagagac gatacctatc aataggacat
     3421 tctcatcttg tggaagggca aattgtgctt gcagggccag tttattagcc tttttacttt
     3481 caatcgtttc aagactataa tttctcacaa ggtactggtc tgtttgacta tcccaactgt
     3541 catagtcgat accattgaca ataccaacca agtcatattt gcgcaactct agtacatgtt
     3601 gcatgttttc gccgaattct tccgttagga tttcgcgagc ataagtttca gaaactgtag
     3661 taaccttgtc agcgtatagg ataccaagct tcatgaagct gatacattca tcatgaaaac
     3721 gtgcaatacc atccaaatag tagctatagt ccatgccaag cgctgaccaa agtgcttgtt
     3781 tgtggaaaat accttgaaaa gccaagttat gaatagtgaa tacatgcttg atattgcgga
     3841 attcttcgcg gtagctatag aaagtacggc aaaggaatgg aagggcagca gtgtgccagt
     3901 catgtgtgtg catgacatcc gggaagaaat gaagtgcttc taacagacga catgtagcat
     3961 gttggaaata gccaaaacgc atggcatcat cgtcatatcc atatagttca tcgcgttcaa
     4021 agaaatcgcg gtgctcaata aaatagaatg ttactccatc aacaacctga ctgtaaacat
     4081 ttgccataga ttggatgtcg ccagcccgca catcaaaact tgatacgtaa tcgaaatcag
     4141 agtgattgtt tactgcaatt tttttgtaga gtggtagaac gactcggaca tctaaaccct
     4201 gttttaccaa ttcttttggt aaagagccaa taacatcagc caatccgcca gtcttgatga
     4261 atggaagtcc ctcggatgct acaaagagaa cagatttttt tgtcatgtta gctcctaaat
     4321 gatttggtgt ggtttcaagt atgaaggcga agtttcgctt cccttgatgt caattgggtg
     4381 aataatttta gttgctttat cgataattgc attttcaata ttcgccccac tcttgattgt
     4441 cacgccattg agaatgattg agtttttgat aacaacacct tcttcaatga taacatcgcg
     4501 gtcaatgact gaattttcga cttgaccatt gatattcgct ccatttgaaa ttaggctacg
     4561 tttgacctct gccaatggac catacaaaca tggtgagcta tcacttgttt gggtatggat
     4621 tggccaatca tgtttgaaga ggagtttgcg ggtatcttgg tcaatcaatt caatttgagt
     4681 attgaagtag gaagcggtcg tattgatgca tgcgacataa tcacgatgtg catagcccat
     4741 aatcttgtac tggtcaactg tatcacgtaa aatatctttt aaccagtaga gagacgacac
     4801 tttgttagca cgtttaatca agtcgataaa tgtagtacgt ttcataatgt aggcttcaag
     4861 ggaaacggga cggtttttat atttaccgtg gttttcttca aaagctacga cacgatggtc
     4921 ttcaccaaat ttgagagttt ggcagccgat gaaattttct ttagcatctg aaacgttttt
     4981 ataaagaacg gtaatatcag catccgttgc gatatggtca tccatgactt ttgaaaaatc
     5041 ctggctatag atgaagtaag atggagcaac tagcacataa tcttttgtag aattttcgat
     5101 gtagtggatg ttttctgtga aggcattaat atcgtgacga tagacactat ttgggtctgt
     5161 tgcgctattg agcaagcgaa gggtaccgcg tttgctgttg atattatagt gtttccctgt
     5221 accaacgtgc tgaattgttg aacgcatttc ggcaggcatg tatacttgga attctgtaat
     5281 tccagagttt gacatattgg atagagcaaa gtcaattagg cgataacgac ctaagaaacc
     5341 gaaagcttgt acgccacggt gcttcatcac gtcaccaaaa tgtacgttgt ttccttcaat
     5401 atttacaatt ccgagagtat ttcttagcat agcagtcctt tcttacttgg tcacattctt
     5461 gtcaatcaag aggatatggt cgatgtctcc tgtgagttta actccgtcgg caattttaat
     5521 attttcagcg acgatgacgt agtcaagttc aacaccttcg ccgataatag caccaggtag
     5581 gatgacagaa ttttttacta cggcattttg gttgacttgc acatctgttg aaataacaga
     5641 atgttctaca gtaccatcga tgattgcccc tttatcaata taggcagatt taaccgttgc
     5701 agttgcaccg atcacctgag caggtgagcc cttgtcttct gaatagattc tccatgagcg
     5761 gtcagaaagg tccaaatctc cagcgtgatc aatcaagtcc atattggatt cccagaggct
     5821 gttaacggta cctacgtctt tccaataacc acggaatgga tgggcaatga gagtgcggcc
     5881 atcttctaag tatttcggaa tgatgtcgtg cccaaaatcg tgagatgaag tgtccaattt
     5941 gtcatcttct tgaaggtact ttttcaaggt cttccaagtg aagatataga tacccattga
     6001 tgctaaattg ctcttaggat tttctggttt ttcttcaaat tcttcaatac ggtaatcttc
     6061 gtccgtattc atgataccaa aacgtgaagc ttctttgatc ggaacttcga taaccgcaat
     6121 cgtggcatct gcttttttct gaatatgtgt atcaaggagt ttgtcgtagt tcatcttata
     6181 gatgtggtca ccagacaaaa tcaatacgta ctctggatcg tgaagatcaa taaaatcaat
     6241 gttctgtgta atagcgtcgg ctgtaccttt gtaaagtccg aaaccttcga ttttttcact
     6301 cggtggtaat acaaagacgc cagatccttg tacatcaagc ccccaacgtt gagactgtgc
     6361 aacgtatgag ttcaagagaa caggctcata ctgagtcaaa actccgacaa tatcaatacc
     6421 tgagttggca cagttactga gaggaaagtc aataatgcgg tactttccgc caaatgcgac
     6481 cgctggtttg gcgacttttt ttgtcaaccc ttctagacgt gttccacgtc caccagcaag
     6541 aatcatagct aacattttat tttgagccat ctaaccactc cttaatatat atgatacatg
     6601 tattatagca tagagtataa aaaaatgaaa gcgtttcctt aaagaaaagt gaattatttt
     6661 catttttctt ttcaacgtct ttcataaatg tatcataaca tattgtattt tgtttgtcta
     6721 tataaaattg tatttatttt tggtcgaatt ttacctcttc aagtaggtaa ttgatgaatt
     6781 tttctcccat gtcggacagc aatgcctttt cgtgttgaat atagaccaat tcgatttcat
     6841 catcaaattc cagaggaatt gatacaatat tatctccgtt aaggtttgaa ttgaggatac
     6901 ctgttgcgat tgtatagcca tctagaccga ttaagagatt gaagagtgtg gcacggtcag
     6961 atacgacaat cgatttttta tggtgttcct gggctagaat ttcttctgca aagtagaagg
     7021 agttgtgaat accttgctcg tagctgagat aggggaaatc taccaaatca tcgagagtga
     7081 ttgactttct ttgtgccaaa ggattggttt tgctgacaaa tacatgtggt ttggcagtga
     7141 agagagttgt ataggatagt cgattgtcat ccaaaagttt cagcagaaca tcacggttga
     7201 aatggttgag gaaaagcact ccgatttctg agcggaaatt cttaacgtca tcaatgattt
     7261 cccaggtccg tgtttcacgt aggaagagtt cataatcttc catttcagtc tctttcaaga
     7321 gtgagacaaa ggcgttgacg acaaaggcat agtgttggga agatacgctg aagagttggc
     7381 gttttgcatt cggattttta tagcgaactt ctaaaagctc tgtctgttca acaatttgac
     7441 gagcatagga aagaaattcc attccatctt ttgtaagtgt aatacctttg gagtttctaa
     7501 gaaaaatctt aatattcatt tctttttcca agtcacgtac tgcattggaa aggctgggtt
     7561 gggtaatgaa gagttgtttg gcagcctcat tcatactgcc tgtttctgca attttgataa
     7621 tatagtataa ttgttggatt ctcatacctc tattgtaaca tgatttgaca aattattcta
     7681 aaaaatgtat aatgctgaaa atatatcctt taactgagtc cagagaggcg aagaagggaa
     7741 cgtgataaat agggcaaata ctgccttctt ttcgtgtaac cttgctagcc agcaaggttt
     7801 ttttctctat atcgactttc ttgttttctg tgtcattgtt atatagaata ggagaaaaga
     7861 tgattttaaa agaacagatg ttactggtgg accaagtcca actagcgcca cgaatttttg
     7921 ctatgacctt gcagggtgat atggtccaag atatgaaaat ggggcaattt atccatattc
     7981 gtgtgccgga tgattccatg ctcttgcgga gaccgatttc tatttccgag attgatcgga
     8041 ataagtctca atgtcggatt atttaccgag tggaaggtca agggacggaa gtcttttcga
     8101 aaatgactgt tgggcagtat ttggatgtca tgggaccgct tgggaaaggg tttgaagtag
     8161 actttctcaa agaaggtgac cgcattctca tcattggcgg aggaatcggt gttcctccct
     8221 tggtggaagt agccaaacgg gctgcgttcc gtggtgtcaa tgtcacgtca gtcattggct
     8281 ttgccaccaa agaggccgtc attttggaag aggagctggc aagttatggg atggtctatg
     8341 tcacaacgga tgatggttcc tatgggcgta aggggaatgt cgcaacggtc gttgaagagc
     8401 tgacaaacga gtttgcagcc atttactcat gtggggcgcc agctatgatg aactatgttg
     8461 atcaacgctt tcaggaacat cctcatgcct acatttcact ggaggctagg atggcttgcg
     8521 ggatgggggc gtgctatgcc tgtgttgtca aacccaaaga gggacaagaa cacgagaaca
     8581 agcgtgtctg taaggaaggt ccagtctttg cgactggtag tctgatttta taggaggttg
     8641 atatgataaa acgattagcg atttccttac caggcttgga cttgaaaaat cccatcattc
     8701 cagcctctgg ctgcttcggt tttggtcagg agtatgcaga ttattatgac cttaaccagc
     8761 ttggatccat tatgattaag gcgactactc gtcatcctcg ttatggcaat gcgactcccc
     8821 gtgtagccga gacaccagca ggtatgctca atgccattgg acttcaaaat ccgggtgttg
     8881 atgtagtctt atccgaaaaa ttaccatggt tggaacagca ttttccagac ttgcctatca
     8941 ttgccaatgt ggctggtttt tccaatgaag aatatgccta tgtgtcaggg aaaatttcaa
     9001 aggctccaaa cgtaaaagct atcgagctga acatttcctg tcccaatgtg gaccacggaa
     9061 ataatggtct cttgattgga caggtgccag aattagccta tcaagcagta aaagcagcag
     9121 tcgaagcctc atctgtacct gtttacgtta agctgacacc aagtgtggca gacataaccc
     9181 tgttggccaa ggctgctgag gatgctggag cgactggctt gacgatgata aataccttgg
     9241 ttggtatgcg attcaatctc aaaactcgac aaccaatctt ggcaaatggt acaggaggaa
     9301 tgtcaggtcc agcagttttt ccggttgctc tcaaactcat tcgtcaggtt gctcaggtga
     9361 ctgatttacc gattattggt atgggtggtg tggatacggc ggacaaggct atagagatga
     9421 tggttgcagg agcttcagct gtaggggtag gaacagccaa ctttacagac ccatttgctt
     9481 gtccaaagat tattcaagac ttgccaaaac gcatggaagt ttatggtatt gatagtttgg
     9541 aaggtttacg ccgagaaatc agacatagat tgggtaagcg ttgacaaaga taaaataaaa
     9601 gagtaatata aagaaaatat ctcctttaac taagtccaga gaggctagga aggtgtcatg
     9661 taatatgctt gaaaggtgat tttcaagctg aattcgtgca catccctgac cgatttggca
     9721 gggatttttg tgtaaatgaa aggattttat gaaagaaaca agaccaatta ttgcccttga
     9781 ctttgcaggt aaagaagaag ttcagtcttt tctggaacag ttcccaccag atgagaaact
     9841 ctatgtaaag gttggaatgg agttgtatta tgcggaggga ccaggtatta tcaattattt
     9901 gaaaggctgc ggtcacagta tttttctaga tttgaaactg cacgatattc ctaataccgt
     9961 cgagtctgct atgaaagtct tggcaaaact cggtgtggat atgaccaacg ttcacgctgc
    10021 tggtggtgtt gagatgatgc gggcagcacg aaggggattg ggtaaggatg ctgttttaat
    10081 agcggtcact cagttgacct ctacctcaga ggagcagatg agaacagacc agaatataca
    10141 gactagtttg caggaagcag ttgttcacta tgctcaaaga gcacaagaag ctggtcttga
    10201 tggggtagtt tgttcagcgc atgaggtggc tttgattaaa gatgctacaa gttctgattt
    10261 tgtgtgcctg acgccaggga ttcgtccgac tggtggtgaa gtaggtgatc agaaacgagt
    10321 tatgacacca gcagatgctg ctcgaatcgg tagtgattac attgtggtag gtcgcccgat
    10381 tacaaaatca gaacatcctt accagaccta tttgtctatt aaagaggagt ggaatcgtgg
    10441 ataagattta tcaggttata atcgatagac ctatcgggta tattgataaa tttggcaacc
    10501 agtaccccat caattacggc tatgttccaa atctattggc tggagatggt gaagagcaag
    10561 atgtttacat catttccaaa gctgttcggc agcccttgac agctttcgag gggaaattgg
    10621 tcgcgattat acatcgcaga gatgatgttg aggataagtg ggttctgaca ggccttgatg
    10681 aaaaacttac cgtgacagaa ataaaagaga aaaccaattt cattgaacag tattttgatt
    10741 catggattga aatggtggac taagagaagt ctgtaagaat tcaacctgga taaatatagt
    10801 ttttataggg ttcatagatt gaaccaatgc tttaggagtg acaggctatt cggcgggatg
    10861 gtcatgttgg aatggtacgc atttagaagg aggtctatta tgacattagc aacagaaatc
    10921 gcatcacact tattggatat taaggcagtt catctgcgtc cagaaaagcc atttacatgg
    10981 gcatcaggga tcaaatcacc tatctataca gataatcgta ttacactatc ttatcctgaa
    11041 actcgaaatt tgattgaaga tggctttgtt cagcgtatcg aagaagaatt tcctgaggtt
    11101 gaggtgattg ccggaactgc cacagcaggt attcctcatg gtgctatcat tgcagatagg
    11161 atgaatttgc catttgctta tatccgtagc aaaccaaaag atcatggtgc agggaatcaa
    11221 ttagagggtc gcatcgtcaa gggacaaaaa atggttgtcg ttgaagacct gatttctact
    11281 ggagggtctg ttctagatgc cgtagcagca gctgagcgtg aaggcgctga cgttattggt
    11341 gttgtggcaa tctttaccta tgaattgcca aaggcagatt gtaattttga taatgctggc
    11401 gtgaaattgg tgacgcttag caattattca gagttaatca aggtcgcaaa agtacaggga
    11461 tacatcaatg cagatggttt gaatctgctt aaaaagttcc gtcaaaatca agaaacttgg
    11521 caggaagact agatttaaaa cgagcccacg cacgatttct gggaacttat cctgtaaaat
    11581 ggtataatag tctctaacgt attttgagaa tacaataatt gaccgtatga ggagaagaac
    11641 atgatttcag ttatcgtgcc ttgttttaat gaagaagagg caattcccta tttttacgat
    11701 gctatggaga aagttcgtaa ggagatgggg gaacaatttg agtatatttt tgtcaatgat
    11761 ggttcaaagg ataggacatt aacggtttta cgccaattgt ctggtcaaga ccgagctgtt
    11821 cgctatcttt ccttttcaag gaatttcggg aaagaagctg ctctctatgc aggtctacaa
    11881 gctgcgcaag gtgagctagt gactgtcatg gatgttgatt tacaagatcc acctgaaatg
    11941 ttgatggaaa tgaaggctat gttggatgga aatccagatt tagattgcgt tggaacccgt
    12001 cgtgtcagtc gagatggtga gccaccgatt cgtagctttt ttgcaaaatt attctacaaa
    12061 ttgatgaata aaatcagcca ggtcgaggta gtagacggtg cccgtgattt ccgtctcatg
    12121 cgtcgtcaca tggtagatgc cattctatct gtgtctgaat acaatcgctt ttcaaaagta
    12181 atttttgctt gggtaggatt cgaaacagaa tacttgccct atgaaaatgt tgagagggtc
    12241 gctggcgaaa ctagttggtc tttctggaaa ttgctttctt attctattga aggaatcatc
    12301 aatttttcag atacaccttt gaatattgcc tcctatacag gtttcttcac cttcctattg
    12361 tcgctagttc taatggtttt tgtggttttc aaaaccttgg tgttcggaga tcctacaatt
    12421 ggctggccgt cgactatttg tatcatccta tttttgggag gtctgcagtt gatgaccatt
    12481 ggtattctcg gtaaatattt ggcaaaagtt ttcttggaaa cgaagaaacg tccgatttat
    12541 attgttaaag agaaaagttc aaactaggag cagtttatgg aacattcagc ttggcacgaa
    12601 ttgataaagg cacagttgcc agagcattat tttggtaaaa tcaatcagtt tttagattat
    12661 gtttatgctg gtggaaccgt ttatccgcct cgtgagaagg tatttgctgc cattcaaaca
    12721 acggatttgg aagaggttaa agtagttatt ttgggacagg atccctatca cggacctggt
    12781 caggcacagg gcttgtcatt ttctgttccg aactcggtgc cagctccgcc atctcttcaa
    12841 aacattttaa aagaattgac ggatgatatt ggcgtcaaac aagatcatga tttgacagca
    12901 tgggcggaac aaggtgtgct tttactcaat gcttgtttaa ctgttccagc aggtcaggca
    12961 aatggacatg cagggcaaat ctgggagcct tttacggacg ctgttattaa ggtggtcaat
    13021 agcttagacc agcctgttgt ctatattctt tggggtgctt atgctcgcaa gaaaaaagcg
    13081 ttgataacca acccaaaaca tctggttatc gaatctgctc atccaagccc tctttcagcc
    13141 tatcgaggtt tctttggagg caagcccttc tcacaagcaa atgcctactt gacttcacaa
    13201 ggcagaacag gaattgattg gctgcgatag tagatggtaa tggctttggt actacaaagg
    13261 agaatatgaa tgttattagt gaaaaatggt cgtgtattgg atccgcaatc tggtttagac
    13321 aaggtttgcg acatcttgat ggacggaagt gagattgtta agattgcgga gtcgatttca
    13381 gaagatgttg agcaagaagt gattgatgcg acaggattag tcgttgctcc tggtttggtc
    13441 gatgttcatg tgcattttcg ggagccaggt cagacccata aagaagatat tcatacagga
    13501 gctctttctg cagcagcggg tggttttact tctgttgtca tgatggctaa cactaatccg
    13561 acgatttcaa atgttgaaac cttaaaagaa gtattggatt ccgctaaaaa agaagctatt
    13621 cacatttata gtgtggcaac cattacagat catttcgacg ggcaaaatct gaccgatttt
    13681 cctgctttgc tggaagctgg agcggtggcc ttttcggacg acggtattcc tttgacaaat
    13741 gctggcgtgg ttagaaaagc tatgaaattg gccaaagagc agggctgtct catcagtctg
    13801 catgaagaag accctgacct gaatggtgtc ttgggactca acgagcaagt ggctgaaaaa
    13861 cattttcatg tttgtggagc aacaggcgta gcagaataca gcatggtcgc tcgcgatgtc
    13921 atgattgcct atgaaacagg tgcgcgtgtt catatccagc atctatccaa agccgagtcg
    13981 gtcaaggtgg ttgaatttgt tcaaggtctc ggagcaaacg tcacggcaga agtagcaccc
    14041 caacattttt cacgaactga ggacttactt ctaataaaag gtgccaatgc taaaatgaat
    14101 ccgccactac gattagaaag tgatcgcttg gctgtgattg atgggttgaa atctggtgtt
    14161 atctctgtca ttgcgacgga ccatgcacct catcatgcag atgagaaaaa tgtagaggac
    14221 cttacgcaag ctccgtcagg tatgacaggt ttagaaacct cactttcatt aggtctaact
    14281 catctggtag aagatggaca cctcactctc ttgcaattgc tggagaagat gactattaac
    14341 cctgccaaac tctacggcct tgatgctggc tatcttgctg aaaatggccc agctgatctt
    14401 gttatttttg acccaaatgc aaatcggttg gtcacagcgg attttgcctc gaagtctgct
    14461 aattctccgt ttgttggtga tagtctgaaa ggacaagtcc tttacaccat tgcaaatgga
    14521 caaatagtat ttggtaaata ggaaatgagc ggattagata gctaattcgc ttatttcgaa
    14581 cctttttgac atgtaagcga ttttaatgtt tttgtttttt gataaaaagt gtataataga
    14641 gatataaaaa atgccgaaga aaggtctatt tatgaaaaag aaaaaaatct tgttgcctgt
    14701 aatgtccaca ctcttgttgg ctccatttgt tttagctcaa caagtacaag ctgctgagac
    14761 aacgactgca gctaccacaa ccaatcagcc agcaacgaca gatgcaactg ctacggtacc
    14821 agcaacatct gttgaaaatg tagcaacgga ggaaactgtt gtccctgctg cggaagaaac
    14881 agtagaggca gtcattatcc acacaaacga cgttcacggt cgcattcttg aagaaaagaa
    14941 tgttatcggt gacgctaagg ctgcggcagt cattgaagaa gaacgtgcca aagtagaaaa
    15001 tacaattgta gtagatgctg gtgatgcctt ccaaggactt ccaatttcca actctacaaa
    15061 aggtgaagac cgcgccaata tcatgaacca agtcggctat gatgcaatgg ctgttggtaa
    15121 ccatgaattt gactttggga tggatcaagc aatcaagtac aaggaaacct tgaacttccc
    15181 gctattgagt gctaacactt acgttaatgg cgctcgtgtt tttgaagctt caactattgt
    15241 cgataaaact ccaactgttg taggcgatga atttgtagtt atcggtgtaa caacacctga
    15301 aactgctaca aaaacccatc cgaagaatgt tgagggagta acgtttaccg acccagtcac
    15361 agaagtcaat aaagtgattg acgaagtgga agcacgtgct ctagcagata atcgtgttta
    15421 caagaactat atcattctag ctcacctagg tgtcgattct acaacacctg ttgaatggag
    15481 aggttcaact cttgctgaag cactttctaa aaatagtaaa ttagccggta aacgtgttat
    15541 tgttatcgat ggtcattccc acacggtaga agcgactacc tatggagata acgttactta
    15601 taatcaaaca ggctcttact tgaacaatat tggtaaagta accttgaaat ccgataaatt
    15661 attgggtgaa gctagcttga tttctgccgc agatactaag aacgtgacgc caaatgctaa
    15721 aatcgcagcc ttggttgatg aaattaaagc aaaatatgaa gctgaaaatg ctcaagtagt
    15781 tattgaaaat aacccagtgg aattaaacgg agaccgctca aacgtgcgtg ttcgtgaaac
    15841 caacttgggg aatgcagtga cggatgcgat ttatgcctat ggtcaaacag gattttcaaa
    15901 caaaactagc cttgctgtga ctaacggtgg tggtctacgt gcaacaattg ctaaagatca
    15961 gcctgttaca aaaggtgata ttattgcagt cttgccattt ggtaacattg tttctcaaat
    16021 cactgtgact ggtcagcaaa tttatgatat gtttaccaag tctttatcat ctacattgca
    16081 agtgaatcca gaaactggcg agatgctttt agatgaaaat ggcatgccgc tctttgaagc
    16141 tagtggtgga ttcctacaca tttcaggagc aaatgtattc tacgatccaa ctcttcctgt
    16201 agaagaacgt gttcttctta tcggtattct taatccagag actggcgaat atgacgcctt
    16261 ggatcttgaa aaaacgtact atttagcaac aaatgacttc cttgctgctg gtggtgatgg
    16321 ctacacaatg cttggcggag cacgtgaaga aggtccttca atggacagcg tctttgctga
    16381 atacctaaag acagctgact tatcagctta tgaggttgtc aacccatatt cacgtatcat
    16441 tcctgtaaat tcaagcattg atacggatga agatggctac ccagacttta tcgaaatttt
    16501 attggatact gatccagaaa atccagcctc aaatccagaa actgttccag ccgaaaatac
    16561 ggattcacca tctaatcaag tacaaaatac ttcagcaacg gacaagaaag ctccagttga
    16621 ttctccgaaa gtaggagata aaaaaacaga agtagcaagt ccagtaaaaa ctacaaaagc
    16681 aggagtcttg ccaaatactg gtgatcagat gaatctgacg ctcagcctgt ttggtttagg
    16741 tcttgcagga ctggccatgg cagtaggtcg tagaaaagaa aattaatcaa tcatctgaaa
    16801 acagggtctt cggactctgt ttttgactgg aggaaacatg caaactgtat ttcaatatca
    16861 aaaagagggg gatcgtgagc agattagttc ttacgttctg cttagcaact ccccacgtcg
    16921 taaacaatta ttgggatttt tacaaccaca aatcgcatca gttgaagtgg atgagcgtgg
    16981 tatcgaagaa cattttatga aacaattcgc tacagatgat tttctcactc gagctgcaaa
    17041 aacctgttgt gaaatttcca aagccaaatc ggacatggat ttggtaactg gtcatctcta
    17101 catttctgcg gatacaatcg tcgtgtcaga tgggcaaatt tttaacaaac ctcaagattt
    17161 gacagaagct gaaatcatgt tacgttccta ttttggaaag agtcatcacg ttgtaacatc
    17221 tgtctgccta cggacaagac actcactgga tgttttttat acggttgcac aaattgattt
    17281 tgtcgactat tatccagccc ttgaagaggt tattcaatca tatatccgtg aaaagcagcc
    17341 actcgacaag gcaggtgcat acggtattca ggagctggat ccacgctttg tgtcgggcat
    17401 ttcaggggat attcacacca ttattggtct acctgtagcc gaagtttccc gcaggatttt
    17461 ttctgaagaa ggttttgaaa aatagcagag ctcaatagct agtttgtggt ataatagtgg
    17521 tcgaaaagga gagtttatga ccgtcgacat tgaaagatac aagcaattgg cccagcagaa
    17581 gcaaggagaa catcgaaaat ttctagcaaa tttgaagaaa aaagcaccta aaaatttgga
    17641 tagaattgtt caagatattc atgccgaagt attttctgaa atcgactgta cagagtgtgt
    17701 caactgttgt aaaacgttgg gaccactctt tacagaagca gatattaccc gaattgccaa
    17761 gcatttacgc atgaaacttc cagtttttga ggatatgtat ttgcgtgtgg atgaggacgg
    17821 agataaggta tttcaatcca tgccctgtcc cttccttgga ggcgataatc tatgtagtat
    17881 ctatgatgct cgtcccaaag cctgtcgaga atttccgcat accgaccgca agaagattta
    17941 tcaaatcaac aatttaacaa taaaaaatac tctgatttgt cccgcagcct atctctttgt
    18001 cgaaaagtta cgggagagaa tttaagagga acaagtaaga caaaatattg attttctgct
    18061 ataataataa aaaaacaagc cgtttttacg gcttttttca atgaatcata tttaggagga
    18121 ccccatggca acaattcaat ggtttccggg ccacatgtca aaagctcgca ggcaggtgca
    18181 ggagaacatc aagcacgttg atttcgtgac cattttggtc gatgcccgtc tgcccctatc
    18241 cagccaaaac cccatgttga ctaagattgt cggtgacaag cccaagctca tgattctcaa
    18301 caaggtcgac ttagcagacc ctgttcgtac caaagaatgg cagagctact ttgaaggtca
    18361 aggcatccgt acccttgcca tcaactcaaa agagcaagcg acggtgaaaa aggtgaccga
    18421 cgcagccaag agtctgatga aagacaagat tgaacgcctc cgcgagagag gtattcaaaa
    18481 agaaaccttg cggaccatga ttatcggtat tccaaacgct ggaaaatcca ctcttatgaa
    18541 ccgtctggct ggaaagaaaa ttgccgttgt tggcaacaaa ccaggtgtga cgaaaggcca
    18601 acagtggctc aagtccaata aggaccttga aatccttgat acaccaggta ttctctggcc
    18661 taagtttgaa gaccaggaag tcggtctcaa gttggctctg acaggagcta tcaaggacca
    18721 acttttgcct atggacgaag tcaccatttt tggtttggac tatttcaagg ccaactatcc
    18781 agaacgtcta caagagcggt tcaagggcat ggatttggag caggaaacgc ctgaactcat
    18841 catggactgg accaagaaat tgggctttcg tgatgattat gaccgttttt acaacctctt
    18901 tgtcaaggac gttcgggacg gtcgcttggg tacttataca ttagacaggg tgagtgactt
    18961 agatggcaac gattaaggaa gtgactactc tgctagctca ggtggacagt ctggacagtc
    19021 ctgtctgggc ggaactatct caggatgagc gagcaggggt ccaggctgcc atcaagaaac
    19081 gccgtaagga gctggataaa gaagtagcag aagatgcccg cttagaagct atgctatttt
    19141 acgaaaaaaa cctttatgaa aatggggtgg agttcatcgc tggtatcgac gaagtcggcc
    19201 gggggcctct agcaggtcct gtggtggcag cggcggtcat cttgcctaaa ggttgtaaaa
    19261 tccgctacct caacgactcc aagaaaatcc ctaagtccaa gcatgaagca atctaccaag
    19321 aagtcatgga gcgagcggtg gcagtcggtg tgggaataaa agatgcagct gtcattgacc
    19381 aggtcaatat ctacgaggcg accaagttag ctatgttgga ggcttttggg aaattaagcc
    19441 agaaacctga ccacttgctc atagacgcca tgaagttgga tacgcccata ccacaaactt
    19501 ctatcatcaa gggagatgcc aatagcctat ctatcgcagc tgcctctatc gtggccaagg
    19561 tgacgcggga taagatgatg gcggactatg acaaggagtt ctcaggctac ggcttcgcca
    19621 agaatgctgg ttatggaacg gcagagcatt tggaaggctt gaacaagtta ggcatcacgc
    19681 ctattcaccg taagaccttt gagcctatta agtctatggt aacaggaggg aaatcgtcgt
    19741 gacggtcttt ttctggattt ttctagcttc cataggctta tttggtctcc tatatttctg
    19801 ggagcgacgg tcgattttta tctcactgtt tgccttgaat atcctcgtct gggccttggc
    19861 atggattatc tcaacaggca ttctcgaaaa caatgaaatc ttgcgcttgc tggctatagc
    19921 agtggctatg gtgctctttt tagccttctt aagtggtcca tttgtcttgc ttttcacact
    19981 ttatctaaat ggttttcaga ttttggagag agaaggggtt cgcttccata atttcctatc
    20041 catgggactg gcggtagccc tgactttcta tctcttcatc gctccctttg tggttcaatc
    20101 cctgtctggt attagtttgt taaacatggt gtttgtctat gttggatttt tagtgtctta
    20161 tgccattatc atcagcatgc tctataccac gtctagcttt gtcaattttg tcaatctctt
    20221 cccaggaaag cttgattatg tagtggtctt aggtgctggc ttgattggtg acaaggtaac
    20281 gccacttttg gcttcgcgga ttgacaaagg catagcgatt tatcagaagc agcctggcag
    20341 taagctcatc atgtcaggcg gacaaggtcc tgatgaactc atagctgaag gtcaagctat
    20401 ggcaaattat gccttggaac agggtatccc tgcggaggac atcattattg aaaaccaatc
    20461 gaccaacaca gaagaaaatt tgaaattttc ctatgccctg atgaaacctg gcagtcgatt
    20521 tgcacttgta accaattact atcacgtttt cagagctctg ctattggcac gacaattgaa
    20581 aattaaatgt atcggctacg gcgcaaaaac caaattttac tttagcctca atgcctttat
    20641 tcgggaattt gtaggctatg tagtcatgag ccgaaagacg catttggtct ttttgagcat
    20701 cgtgtcagcc ctttacctga tcggagtgat catcagttta atgcattaaa aacagcctct
    20761 aggtgactag aagctgtttt cttataacat aaagtagaca atggcgatat attgaagtgc
    20821 cgaagctagt aggataaaga gatgccaaat catgtgaaag tagggctttt tcttggcgta
    20881 gaagagggct ccaatagtat aagataatcc accagccagc atgagaatgc caaatgccat
    20941 tccagtcttt tgaagaatgg ctggcaagat aaagacaact agccagccca tgaaaagata
    21001 gaggaagagg ctgaattttt cgtttatttt tttagcaaaa attttataga gtataccgaa
    21061 aatagtcaat ccccattgca aacctacaat cagataacct agccaaccac cgaccaagga
    21121 tagggcaaca ggtgtgtagc ttccagcgat agcaatgtaa atcatgctat ggtcgataat
    21181 cctcaggatg tatttgtgag tagaagcata gggcatggag tggtagatgg aagaggatag
    21241 gaacatgaga aataggctga tgacaaagat ggacatacca atcacagcgg tcaggttata
    21301 atcttgatag gaatgaatag cagtaacggg gagcaagacc aacataagca gggctccgac
    21361 tgcatgtgtg acactgttag caacctcctc tccaaaggat agttgcaggc tcaatttcaa
    21421 actaggattc atggtgactc ctttctaact ccatagtgag ttgacgggtt ttgtagtaaa
    21481 gtttgacaga ttgacaagcg tagcgaatga tgtcaggggt ttcttcattt tcctcgtttc
    21541 gcatgcagtc aacaaaatga ttgtagagtg tttctgaatg actcgattgg agaagaagat
    21601 gtaaggtctg actgatttct tgaatagaaa agacattttt taagatagtc aaggcaatca
    21661 agcgggcaac ttgctgtttt tgatattttt tcttaacagg tttttcaatc tgtttatgtt
    21721 tgacatagtt attgatcatg gcagcagtca atactcttcg aaaatcaaca ttatccgttg
    21781 tcaacttgcc ttgatgaacc ccagttctat catcggtttc gtttcctagg ctactgttga
    21841 ttttcattga gtataaactt ttctgttcaa ggctttcctg agaattgact acttgattaa
    21901 catacagcag aacttggtcc aaatacaaat ctaaattagg taattcatgc cagtagggga
    21961 tggtctgttt catgattatc cactttcttt tatctagtaa tgataactag attgtacgat
    22021 atccaatatt aaaagtcaag aaaaaacctt ctggacccaa aatcacagaa ggttagttgc
    22081 ttatggttgg ataatatttt ctggtaaagc catcttatag agtgaaaagg caccatctag
    22141 gtttttaaca ccaaagccct tttctttcaa gattcgttcg gcaatataag aacgaagtcc
    22201 actatggcag atgacaatgt attcttttga agcatccagt tctccaattc gctcacggag
    22261 ttggtccaaa ggaatgtgaa tgaattctct gaattgccca tttgcgacct caccaggatt
    22321 gcggacatct aatagaactt taccagtagc taactcgtcg tctagttgat gccactggat
    22381 gttttggcta atcccttctg tgatgttcat agcagcgtag ccaatcatat tgacagggtc
    22441 tttggcagag ccaaatggcg gagcgtaggt caattctagc tctggtaaat caaaaattgt
    22501 cagtccagcc ttgatagcag tggcaaggac atcaatccgc ttatcaatac ctttttgacc
    22561 gacggcttgc gctccgaaaa tttttccatc agtcggtgag aaaatgagtt ttaaaacaat
    22621 atcagtcgca ccaggataat aacctgcatg gtcttttcca gttgtatgaa taatggcatg
    22681 ctcatagccg agtgattgaa cagtacgttc gcttaaacca gttgaggcgg cagctaggtc
    22741 aaagacttta acgatagctg ttccaataga acctttgttt tttctatcaa gtccagcgat
    22801 aacatccgcc acctgtctgc cttgtcggtt ggcaggagaa gccagagaaa tcaaggcatc
    22861 ttgacctgta atttcttgtt taacaacaat agcatcacca acagcataaa tattattctg
    22921 actggtttgg tagtttttat ctacgagaat accaccacgg agacccagtt caattccagt
    22981 ttttttagcc agttggctgt cgggagcaac tccgactgag agaatggtca catcagatga
    23041 caaggtttgt ccgttagaca attctagaac ctgtcctttt tcatgaaaag cagtaacaga
    23101 ctgcccagtg ataacctcga tacctttatt ggttagctct gcttccacat atcgtgccat
    23161 ttctaagtca aaagtaggta aaacatgggg agcttgttcg acaagcgtga ccttccagcc
    23221 ttttaaagct aagttttccg ccatttccag accgataaaa cctgcaccaa ttaccacaac
    23281 atgaccagac ttgatctggt ctagtttaat cataatcttg tccaaatcag gtacgttccg
    23341 aagagtaaag agattgtttg cttcttcaat accagataga tttggaatga caggcttggc
    23401 gccgggtgat aagattagtt tgtcgtagct ttcaatcaac tcaccagtag gcgttttgac
    23461 tgttacagtt tgctgctcag catcgatgct aataacttca tggaaggggc gtacatctag
    23521 attaaagcgt tgtttcaaac tttctggcgt ttggaggagc aagctatcat gttctgctat
    23581 ttcaccagaa atgtaataag gtaaaccgca gttggcaaag gaaacataag gtcctttttc
    23641 gaatacgatg atttcagcat cttccataag acgacgcaga cgagtagctg ctgacatacc
    23701 accagcgaca ccacctacaa taattatttt catgctattc ctcctgtcca ggcattcata
    23761 ccgcctttaa tgttgattgc ttgatagcct tgagatttaa gatattctgt cgcccgttgg
    23821 ctacgcatgc cagattgaca gatgacaaga tagttcttgg ttttgtcgcc tttaaattgg
    23881 tcaagttgac ttaaaggctg gttgatagct tgtggaatat gtccactagc aaattcgtcg
    23941 acttcgcgaa catcaagcaa aatagcattc gatgattgta tcgcttgttc tacatcagtt
    24001 atcggaagac tatctgagtt tgaaaacatt cctgaaaata attgtttaaa cattgttact
    24061 cctttttact cctttttata cccatggggg tatctaaaat atatcaagga attcaaaaga
    24121 tgtcaaggta aaaaattgac atttttcact tcaagcagta ctatataggg tatgaatact
    24181 gataagaaaa agatgataaa ccgtttgaaa cgggccgaag gtcaactcag aggcattcag
    24241 aaaatgattg aagaagatca ggaatgtatt gatattgtaa ctcagttgag tgctgttcgt
    24301 tccagcatta atagtatgat gggaatggtt attgctcaga aggtacaaga ttgtatcact
    24361 catccatctg aaaaccctga agaacaagaa gctcgtcttg ctcaagctat caatttaatc
    24421 ataaaaaaat aggagcagaa gtatgccaac agaaaaagaa aagatgatag ctgggcaatt
    24481 ttatatggca gcagatgctg aattgagaga gatgcgaacc tttgcccgcc aacagatgaa
    24541 aaaatttaac gaagaactag atggacaagc ccgctctgag cgattgaaaa actggttggg
    24601 agctacaggt gagcggattt acgtggagcc aaatttctac tgtgactacg gtagcaatat
    24661 tcatgttggt gaggattttt acgcaaattt taactgtacg atgttggatg tttgtcccat
    24721 taccattggg aaaaatgcca tgcttggtcc caatgtacag ttgctaactc cacttcatcc
    24781 actagatcct gatgaacgaa attctggttt ggaatatggc tctccaatta ccattggtga
    24841 taatttctgg gctggtggtg gtgtgacaat cttacctggc gtgaccttgg gaaacaatgt
    24901 agttgttggt gctggttccg ttgtgaccaa gtcttttggt gataatgtcg ttttggctgg
    24961 caatcctgcg cgtgttatca aagaaatacc taaaaaataa ttcttcttcg ggagaatttt
    25021 ttctttgcct tgcgaataga ctaagtgagg tgaagaaaat gaataatttt ga
//
LOCUS       DASFSW010000012        24405 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.012, whole genome shotgun sequence.
ACCESSION   DASFSW010000012 DASFSW010000000
VERSION     DASFSW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24405
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.012"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..247
                     /locus_tag="U1099_000421"
     CDS             <1..247
                     /locus_tag="U1099_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEM4866769.1"
                     /translation="HAMVLTGVDLDDNEQPLKWKVENSWGDKVGDKGYFVASDSWMDE
                     YTYQIVVRKEFLTQEELAAYQAQPQVLAPWDPMGALA"
     gene            complement(297..998)
                     /locus_tag="U1099_000422"
     CDS             complement(297..998)
                     /locus_tag="U1099_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEM4866770.1"
                     /translation="MNNDSFIINQVDNTALNRFFGKIYGVVAMGIGLSALVSFLAVTV
                     FQSLLLSLLSAGSIIMMLIMIGQIALVVSASAMAAKNSPMALPMFLAYSVTNGITISM
                     ILMFYTSETVVLAFVSAALMFAIMAVIGMTTKKDLSGMAQALRAALWGIIIASVINIF
                     LRSSGLSFMMSIISVLVFSGLIAYDNQRIRNVFEQTGGNVGQGWVVSMALQLYLDFIN
                     LFLNLLRIFGGLSRD"
     gene            complement(1020..1502)
                     /locus_tag="U1099_000423"
     CDS             complement(1020..1502)
                     /locus_tag="U1099_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM4866771.1"
                     /translation="MVAYKQDKEYMDYVGHLIATPKVQKLGKIPHHYYSTRLEHSINV
                     SYTSYKIAKKFGWDAKSTARGGLLHDLFFYDWRDTKFNKSHAWVHPRIAKRNAQKLIQ
                     LNKLEEDIIVKHMFGATIAPPRYKESWIVTCVDKYWAVREWSLPMQHKWKNRKVFRFQ
                     "
     gene            complement(1550..2290)
                     /locus_tag="U1099_000424"
     CDS             complement(1550..2290)
                     /locus_tag="U1099_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEM4866772.1"
                     /translation="MDIIRSKTNNLVKQVKKLQQKKYRTSSYLIEGWHLLEEAMEAGA
                     NIEHIFVVEEYFEKVAGLANVTVVSPEIMQDLADSKTPQGVVAQLALPSQLLPETLDG
                     KFLVLEDVQDPGNVGTMIRTADAAGFDGVFLSDKSADIYNMKVLRSMQGSHFHLPVYR
                     MPISSILTALKSNQIKILATTLSSQSVDYKEITPHSSFALLMGNEGQGISDLVADEAD
                     QLVHITMPGQAESLNVAIAAGILLFSFI"
     gene            2455..2736
                     /locus_tag="U1099_000425"
     CDS             2455..2736
                     /locus_tag="U1099_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEM4866773.1"
                     /translation="MRKVKMIASGIVQGVGFRWSVQYLAMEIGDIAGRVWNNEDGTVT
                     ILAQSENAEKLSHFIHEIRKGPSRMAKVTYLDVTLANFEDYKDFQVSYR"
     gene            2827..3720
                     /gene="yidC"
                     /locus_tag="U1099_000426"
     CDS             2827..3720
                     /gene="yidC"
                     /locus_tag="U1099_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006269468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM4866774.1"
                     /translation="MLSGMALSTLLFLSGCVQTDKSGNPTGLIWDWLGQPMSNLIRFF
                     AEDQALGFGLAIIIVTLLVRFIILPLGIYQSWKAAIQSEKMNYLKPILGPIQERMKNA
                     SSQEEQLAAQQEYFATQKQYGVSMLGGIGCLPMLIQMPFFSAIYFAARHTPGISEATF
                     LGINLGSTSLILTVVAGILYYAQSLLMQVGMDEEQKKQMKTMALMNPIMIMMFSFGSP
                     AGVTLYWVVGGLFGILQQAITNFILKPRIRKQVEEEFKNITVSATPRKTKDVTPTASA
                     VIEEKTSNKKRNRNAGKQRSR"
     gene            complement(3804..4286)
                     /gene="greA"
                     /locus_tag="U1099_000427"
     CDS             complement(3804..4286)
                     /gene="greA"
                     /locus_tag="U1099_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEM4866775.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISTIETKIRYAEIVNSDAVAADEVAIGKTVTVQEVGETE
                     EEVYHIVGAAGADAFANKISNESPIGHALIGKKTGDVATIETPAGSYDVKILKVEKTK
                     "
     gene            complement(4360..6177)
                     /gene="mltG"
                     /locus_tag="U1099_000428"
     CDS             complement(4360..6177)
                     /gene="mltG"
                     /locus_tag="U1099_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEM4866776.1"
                     /translation="MTKDTNEKNTQSSGFRDQILRDLEELKVKRLAEQSADVLVDKRN
                     EVINELQPSLAEEKAPHRETEIVKVEGIAFPDSYLSEEQQESEVMTESIEEPTSETLA
                     TPETFVIEKEVVSSPVPQDTVERNLEKLRNLVAANTAGFDEVPSSSSVSEPVESVLPG
                     NSPLEDTFLEFPTEDVSAVTGDTEIISLEETIIAPKTTAIVPEVDKQMQETQPRRRTT
                     HRTSKQRRKKQDNAAKHIVSVIMSIVVVAVLVTGVTGYMWVKSSLEPVDAKATETIQV
                     EIPEGTSTLEIGKILVDNKLIKNATIFNYYSKIKSYNNFQSGFYNLKQNMSVDDIAKA
                     LQESGTPTAQKEAAGKVLIVEGYTLTQIAQAITDNTNTKDKNDKTPFTAEQFMATVTN
                     QDFINRMVATYPKLFASLPAADSGVIYQLEGYLFPAVYEYSDETTIEELVEQMIAAMD
                     NRLQPYYETITAKNLTVNEVLTLASLVEKEGSTDEDRRNIASVFFNRLNAAMPLQSNI
                     AILYAQGKLGQETTLAEDAAIDTSIESPYNIYWTPGLMPGPVDSPSLSAIEAVINANT
                     TDYLYFVADVTTGNVYFTNNIDEHNQNVAKYVNAHLNNE"
     gene            complement(6249..6734)
                     /locus_tag="U1099_000429"
     CDS             complement(6249..6734)
                     /locus_tag="U1099_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866777.1"
                     /translation="MEIRFATPSDLEQVVLIENANFSKEERIAESVLATYLNTLSKTC
                     LIMEHDGEIAGYLLSCPSVSQTVTDDIFYLTESDMPTGSHLAIASLSVADSYKGQGVG
                     TLLLAAIKEVALAGGFEGVSLTCKGYLISYYEMNQFEDFGPSLSQFGGQRWSDMYWKA
                     L"
     gene            complement(6790..8121)
                     /locus_tag="U1099_000430"
     CDS             complement(6790..8121)
                     /locus_tag="U1099_000430"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEM4866778.1"
                     /translation="MTKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGIQILPFDEKNITADVELIAGNAFRPDNNVEIAYVDAQGYTYKRYHEFLGEFMKG
                     FTSLGVAGAHGKTSTTGLLAHVMRNITDTSFLIGDGTGRGSANAQYFVFESDEYERHF
                     APYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQVKKALLVFGEDEQLRRITANA
                     PIYYYGFEENNDFVAYDLKPSTSGSQFKVRHGEEELGEFQIPTFGKHNVMNATAVIAN
                     LYIAGFDLQLVAEHLKTFGGVKRRFTEKIVNDTVIIDDFAHHPTEIIATIDAARQKYP
                     SKELVAIFQPHTFTRTIALLDEFADALNGADAVYLAQIYGSARETDNGQVKVEDLAAK
                     INKKGGLVTVENTSPLLDHDNAVYVFMGAGDIQSYEYSFERLLSNLTNNVQ"
     gene            complement(8123..8785)
                     /locus_tag="U1099_000431"
     CDS             complement(8123..8785)
                     /locus_tag="U1099_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866779.1"
                     /translation="MIRHEKRFPLVADDEVLVGENPIMSLYDESDLISNIRGLYQEKE
                     FSWSTESQRVASAKPVAQTEDELLPPLFEAKPSHYSRKERLQQLTKTKPSPVKTQGQL
                     AREQAREDLKKKRSATYLRDEKPAPAKVVVKPPVVSTTESKAGRLTRLADNLRQTDYI
                     LADMPAVYSLKKEDREQEQVIKKNSYDFLRKSQVYNYPERRQQRDRQVAQELNLTHIE
                     EE"
     gene            complement(8864..11956)
                     /locus_tag="U1099_000432"
     CDS             complement(8864..11956)
                     /locus_tag="U1099_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM4866780.1"
                     /translation="MGRMMPGRIRQEGIDLYEAGKLTVLHSENGKMVLDIAGERFTYG
                     DADSDLQCSCQLFQSKGYCQHLAATEYFLKNDASGKDMKQSLKEDSEQHKETVRRTYF
                     GGLFLDEILRPEPELGLKYQLSVEGSLLPYDRQIDWTLKITRLPDTRSYIVRDIGAFL
                     RIVKANGHYQIGKNYYEQVTYENFDEPSQALLDFLWALVPEKVGIDSDILTHFGRHFR
                     LPQAYFEEGLELLNQLEHFNFSYQQQSYSSLKVLPLTGEEGLYHFEVTVHTQMIEMVI
                     HEKTVRPLFHGRYLLVNGTVYSVNRHQEHLIYQISELVPTESGLRKVQVDFPDQNRLA
                     LSLLDLQTIGTVKAPKRFIIHDFKPEFHIQMEANESLSLQLVLDFDGRKVRSEEDLAL
                     LPFASHFQHLERVYQAIRLAGFRGTYHAQRAALSQQELYPFFQQQLPILQKMGQVHLA
                     ERLQALYIEAKPQLEIVRNGSLLDISFDLSGIEQSEIDQAITALLNQEDHYTSPSGKV
                     FVFDEETKKISQTLIYLRARHGKEGQVQVHALAGYQLAQSLSQFNQVSFSKEFEEMAS
                     YLAQPDLFPLPAVEVTTSLRDYQQTGLKWLTMLDTYGFGGILADDMGLGKTLQTIAFL
                     SSRMTKDSKVLILAPSSLIYNWLDECKRFAPSLDVAVVHGNKEQREEIIANGHQVLVT
                     SYPSFRQDVALYKQERFDYLILDEAQVMKNAQSKIAQLLREFEVGNCFALSGTPIENH
                     LTELWSIFQIVLPGLLPGKQDFGKLAAKDIARTIQPFVLRRHKEDVLQELPDLIEVNV
                     LNELTDEQKAIYLAQLQQMRTQIAGADDAQINRSKIEILSGITRLRQICDTPSLFMEE
                     FSGESGKLNSLKELLLQLKEGEHRVLIFSQFRNMLEKIEEQLVEIGMTSYTLTGSTPA
                     NQRQEMTQAFNAGSRDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAISRAHR
                     MGQTEKVECYRLITRGTIEEKIQELQENKKNLVKTVLDGNESRANLTVEDIREILGIE
                     "
     gene            complement(12096..12404)
                     /locus_tag="U1099_000433"
     CDS             complement(12096..12404)
                     /locus_tag="U1099_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866781.1"
                     /translation="MEFFGELLVEFLTGLADFDEKKHPPFGIRYWLGWLGVLIHVLLL
                     ALLTCVTVFFFKFFLVGKGLINVVVAVVFLLFALFWLWKSGKTILKMWQATIYYLAIH
                     "
     gene            complement(12406..12744)
                     /locus_tag="U1099_000434"
     CDS             complement(12406..12744)
                     /locus_tag="U1099_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM4866782.1"
                     /translation="MVAVPIWRSFLILLSFSSFFLVFYEDSQYKPFGFRYWLGLVACI
                     WLNFVTLASYFIAFTGGSIMVYNQYKQPLVVIFLFCLFVASMLTLLSFKAIKMMIRRT
                     KYYRQVRKVG"
     gene            complement(12812..14122)
                     /gene="der"
                     /locus_tag="U1099_000435"
     CDS             complement(12812..14122)
                     /gene="der"
                     /locus_tag="U1099_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEM4866783.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQAEIAMDEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILVVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAII
                     ENLPVQEAEENPDIIKFSLIGRPNVGKSSLINAILGEERVIASPVAGTTRDAIDTHFT
                     DPEGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIERSDVILMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKKWEDDIRDQFQYLSYAPIIFVSALTKQ
                     RLHKLPEMIKAISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVVFVNEEELMHFSYMRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14141..14854)
                     /locus_tag="U1099_000436"
     CDS             complement(14141..14854)
                     /locus_tag="U1099_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM4866784.1"
                     /translation="MNETIDLMLNHTSVRRFTEEPIEAEHLQAIISAGRAASSWKNFQ
                     SYSIIVVQLEEKKQALYDLVPQPAILQAQAILVFVGDPNRASKAAQLHGSDFDAKGTE
                     NLLISSVDASLAGQNALLAAESLGYGGVFIGMIRHKAMAIAELFNLPDYTYPIFCIAL
                     GRPAQNHPVKPRLASETIVFQEEYVEQGVEVIQAYDQVQTAYAGARQTETWSERMVAQ
                     FGQAEQPETKSILEKNKLL"
     gene            complement(14851..15750)
                     /gene="dnaI"
                     /locus_tag="U1099_000437"
     CDS             complement(14851..15750)
                     /gene="dnaI"
                     /locus_tag="U1099_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEM4866785.1"
                     /translation="MKSVQDRLSQTTNPSPKSYQQLYQEIVSDPEVAAFIKKEGLTQQ
                     EITLSISKFLEYISQRDLFVKQDEAYIAKGYQPVLVMNEGYADVSYLETEELVEYRRL
                     EAIKNRIQLINMPASLKNVTVADIDKSDENRVEVMLAIADFVKRFEEKPKGLYIYGNF
                     GIGKSYLMAYLANLLSKTHLQSTTMLHYPTFVVDIKNAIKDGSVKERIDEIKMAQVLV
                     LDDIGAEQHSPWVRDDVLQVILQYRMQENLPTFFTSNFSFDDLERHFASGKSGDETWQ
                     AKRVMERIRYLARDLHLKGNNRR"
     gene            complement(15750..16901)
                     /locus_tag="U1099_000438"
     CDS             complement(15750..16901)
                     /locus_tag="U1099_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation/membrane attachment
                     protein"
                     /protein_id="HEM4866786.1"
                     /translation="MKPNDLFAYIKISPFTPDIVSLSQCYQPIIGFDALALYYYFYSF
                     ADQGQGRYKWTVILNHMDFGMNRLEKALDVLTAMGLLELYRADELTGLALQAPLTVKS
                     FLAKPLYKNLLARKIGESSMENLLVHQPNQEQNISKTFSQVFTMDGQAPVVFEPKHDF
                     DWSAFKALMAKDKLLFQDEAEDIIALSYIAEQAGWTWLETYRQAKATAIGQTISTKRL
                     QQSRQSLPTENANLTAQEQAIVREAKSKSSLTFLSFIKEQRKAAVTGTERKCLTDLAN
                     LGLLDEVINVLVLYTFNKVDSANLNEKYAMKLGNDFSYKGSVSAEAAVLYLRELKTGQ
                     VVTKSSQPQKTNVPEWSKEEVQQGQTQEGQAKLAALYRELEEMENKGGS"
     gene            complement(16888..17379)
                     /gene="nrdR"
                     /locus_tag="U1099_000439"
     CDS             complement(16888..17379)
                     /gene="nrdR"
                     /locus_tag="U1099_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEM4866787.1"
                     /translation="MRCPKCQSLKSSVIDSRQAEDGNTIRRRRSCDQCGQRFTTYERI
                     EEKTLVVVKKDGTREQFSREKIFNGIIRSAQKRPVSTDDIDEVVNRIEQKVRAQGDSE
                     IESDVIGNLVMEELVELDEITYVRFASVYRSFKDVGELENLLKQMISKGSKVKPGKKD
                     ETK"
     gene            complement(17451..18581)
                     /locus_tag="U1099_000440"
     CDS             complement(17451..18581)
                     /locus_tag="U1099_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM4866788.1"
                     /translation="MNTSFKEKVILILLSGATLLAVLNWSSIYGAIQQLLGSMNSLFI
                     GAIFAFILNVPMKKLEDQFEKISFLKKSKRSLAIVGVLIGFALIVTGLVVIVLPTLVT
                     TVSELVSVSSTAIPKSVNALTNFLESNGLLSGQIGDQIASFLDQLKNLTLISSLVTPI
                     LSGLVSNVTGIFSNTMTLIMAFFFTLAILGSKEHLQAMTRKFLQATLPEKAVRVVNYI
                     GEVIVDTYDRFLMSQIVEACIIGTLVFVSYSLSGIPYASMAGILAGVLSFVPYIGPFS
                     ACLISALFVAVQDPLLALWSIALFQIIQLIEGNVIYPRVVGQSVGLPTLFTLAAALIG
                     GNLFGLLGMVFFTPIFAVVYRLVREWVASRLKKQGELLAESE"
     gene            complement(18671..19360)
                     /locus_tag="U1099_000441"
     CDS             complement(18671..19360)
                     /locus_tag="U1099_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEM4866789.1"
                     /translation="MAKKILIAGKERNLSHFVSMELQKKDYLVDYASTGKEAMSLAHE
                     TDFDLILMSFQLSDMSSKELAAELLAIKPATVMIVVVEPTEVSQYGEEVLSYAVSYVV
                     KPFVISDLVEQISAIFRGRDFIDNNCKQVHMHAAYRDLKVDFQNRTVTRGDELINLTR
                     REYDLLATLMNSPEPVSREQLLERVWKYEAASETNVVDVYIRYLRGKLDLPHQDSYIK
                     TVRGVGYAMRD"
     gene            complement(19442..20869)
                     /gene="gndA"
                     /locus_tag="U1099_000442"
     CDS             complement(19442..20869)
                     /gene="gndA"
                     /locus_tag="U1099_000442"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEM4866790.1"
                     /translation="MTKANFGVVGMAVMGRNLALNVESRGYSVAIYNRSADKTEDVVA
                     SNPGKNLVPSYDVESFVASIEKPRRIMLMVQAGPGTDATIQALLPHLDEGDILIDGGN
                     TFYEDTIRRSKELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGAPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSVDEMAD
                     IFTEWNKGELDSYLIEITADILKRKDDQGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKDERVAASKVLPKPAPFAYEGDKAELVEKIRQALYFSKI
                     MSYAQGFAQLRVASKENNWNLPFGEIAKIWRAGCIIRARFLQKITDAYGRDEDLANLL
                     LDEYFLDVTAKYQQAVRDVVALAVQAGVPVPTFSAAITYFDSYRAENLPANLIQAQRD
                     YFGAHTYNRKDKEGIFHYDWYSESK"
     gene            complement(20966..21505)
                     /locus_tag="U1099_000443"
     CDS             complement(20966..21505)
                     /locus_tag="U1099_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEM4866791.1"
                     /translation="MFHIYDIQKNLDGISFDKTLDLQEELQARNGEVLGLSPVEVTGN
                     IRFESGFFFLDYQMTYDITLASSRSLQPVVLHEVQNVNELFVANEAVLKEQDLIDEDM
                     VLVVEDDHIVLDESVADNILLAIPIKVLTPEEEAGQELPSGQAWALMTEEDFQQKAQE
                     KKEANSPFAQLQGLFGDED"
     gene            complement(21515..22432)
                     /gene="trxB"
                     /locus_tag="U1099_000444"
     CDS             complement(21515..22432)
                     /gene="trxB"
                     /locus_tag="U1099_000444"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEM4866792.1"
                     /translation="MYDTVVIGAGPAGMTAALYAGRSNLKVALLERGIYGGQMNNTAE
                     IENYPGYDHISGPALAEKMFEPLEKFGVDHIFGTLVRIEEEGQIKKVITEDGVLETKT
                     VVLAMGAKHRLLGIPGEDTYNSRGVSYCAVCDGAFFRGQKLLVVGGGDSAVEEALFLT
                     QFAESVTIVHRRDQLRAQKVIQDRAFANEKINFIWDSVVEEIKGDDLRVQSVVIKNVK
                     TEEVSELDFGGVFIYVGLDPMTDTVADLGITDEAGWVITNEKMETTQSGIYAIGDIRQ
                     NQLRQIATAVGNGAVAGQEVYNYITELAE"
     gene            complement(22446..22670)
                     /locus_tag="U1099_000445"
     CDS             complement(22446..22670)
                     /locus_tag="U1099_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEM4866793.1"
                     /translation="MLQNILSFYLQGLLIASLLIITSSLVWFIWRATKGVDKTLQERQ
                     DFLFDLLMINVMTIPIAAFGVVGILLMFKA"
     gene            complement(22688..24031)
                     /locus_tag="U1099_000446"
     CDS             complement(22688..24031)
                     /locus_tag="U1099_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM4866794.1"
                     /translation="MKFTDMKLKPYIQEALKEINFVQPTEVQEKLIPVVLSGRDLIGE
                     SKTGSGKTHTFLIPIFQKLSEELDQVQAVITAPSRELATQIYQAARQLASHSDIEVRV
                     TNYVGGTDKNRQIDKLQASQPHIVVGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEQIKTHTVISDTIE
                     NWLISTKGRDRNAQILEVTKLMQPYLAMIFVNTKTRADELHSYLTANGLRVAKIHGDI
                     PPRERKRMMNQVKNLDYQYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGIAITLYQPSDDADIRELEKLNIKFLPKEMKNGEFVDTYDRDRRANREKSREK
                     LDLEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRVEARAKGRAERKAKRQTF"
     gene            complement(24111..24374)
                     /locus_tag="U1099_000447"
     CDS             complement(24111..24374)
                     /locus_tag="U1099_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HEM4866795.1"
                     /translation="MIGVKITKKTVEKTIANAVPILGGLISGGLTYFTFQPMGGMLAD
                     TFVKKINGDFDGELELTEAFKASLEEEKELGIIEAEFTEASES"
BASE COUNT         7056 a         5564 c         4584 g         7201 t
ORIGIN      
        1 ccacgctatg gtcttaactg gtgttgattt agacgataat gagcaacctc tcaaatggaa
       61 agtagaaaac tcttggggtg acaaggttgg ggacaaggga tacttcgtag catcagatag
      121 ctggatggac gagtacactt atcagattgt tgtccgaaaa gaattcctca cacaagaaga
      181 actagcagct tatcaagctc aaccacaagt ccttgcccca tgggatccaa tgggagctct
      241 agcataacaa acgacatata aaaaaagagg gcacccctct tttttatatg tcataattag
      301 tctcgactca atccaccaaa gatacggagt aagttgagga aaaggtttat aaagtcaaga
      361 tagagctgaa gcgccataga tacgacccaa ccttgaccaa cattaccacc agtttgttcg
      421 aaaacattgc ggatacgttg gttgtcataa gcaatcaaac ctgagaatac caaaacagaa
      481 atgatagaca tcataaagct tagaccagag ctacgaagga agatattaat aacacttgca
      541 ataataattc cccatagagc tgcacgcaat gcctgagcca taccagaaag atccttcttc
      601 gttgtcatcc caattacagc catgatagca aacataagag ccgctgacac aaaggcaaga
      661 acaactgttt cgctggtata gaacatcaag atcatgctga tggtaatccc atttgttact
      721 gagtaggcaa gaaacattgg tagagccatt gggctatttt tagctgccat tgctgaagca
      781 gaaacaacca aggcaatctg accaatcata atcagcatca tgatgatgga gcctgcactc
      841 agcagactaa gcaataaaga ttgaaagact gtaacagcta ggaatgatac taatgctgat
      901 agtccaattc ccatagcaac cactccgtaa atcttgccaa agaaacgatt gagggcggtg
      961 ttatccacct gattaataat aaaagaatcg ttattcatga tattctcctt taaatcatct
     1021 tattgaaaac gaaagacctt gcgatttttc cacttgtgtt gcataggcag actccattct
     1081 cttactgccc aatacttgtc aacacaagtc acaatccaag actctttata gcgaggagga
     1141 gcaatcgttg caccaaacat gtgcttcaca ataatatctt cttctaattt attaagttga
     1201 atgagtttct gagcattgcg cttagcaatc cgtggatgaa cccaagcatg acttttatta
     1261 aacttagtat ctctccagtc atagaaaaag agatcgtgta atagcccacc gcgagcggtc
     1321 gattttgcat cccatccgaa ctttttcgca attttatagc ttgtataaga tacgttaatg
     1381 gaatgttcca accgtgtgga gtaataatga tgaggaatct tccccaattt ttgaacttta
     1441 ggtgtagcaa ttaaatgccc cacatagtcc atgtattctt tatcttgttt atatgcaacc
     1501 attctttgaa cctcgttgga ttattatacc acactatagt acaattggct taaataaagc
     1561 taaatagtaa aattcctgct gcaatcgcca cattgagact ttcagcctgt cctggcatgg
     1621 tgatgtgaac aagttgatcc gcctcatcag ccactaaatc agaaattccc tgaccttcgt
     1681 ttcccatgag caaggcaaaa ctagaatgtg gcgtaatttc cttatagtca accgactgac
     1741 tagatagggt cgttgccaag atttttatct gattgctttt aagagctgta agaatggagg
     1801 aaattggcat ccgataaact ggcaaatgga agtgactccc ctgcatggaa cgaagcactt
     1861 tcatattata aatatcagcc gacttatccg atagaaatac tccatcaaaa ccagctgcat
     1921 cagctgtacg aatcattgtc ccaacatttc cagggtcctg cacatcttcc agaaccaaaa
     1981 attttccatc caaagtctca ggtagaagtt ggctcggcaa agcaagttga gctactaccc
     2041 cctgaggtgt cttagaatca gctaaatctt gcataatttc aggactgaca actgtcacat
     2101 tggctaaacc tgcaaccttt tcaaaatact cttccaccac aaaaatatgc tcaatattgg
     2161 ctccagcctc catcgcttcc tccagcaaat gccagccctc aatcagataa gaagatgtcc
     2221 gatatttttt ctgttgtaat tttttaactt gtttgaccaa attgttggtc tttgagcgaa
     2281 tgatgtccat gtttgtccct tttctttttc tgttcaacaa ctttattaat aaaataccgg
     2341 gtacgcttga taccaaatat atcttattcc gtcaagaaat catcatcaag atggtgaaca
     2401 ggattaccca accatgatat aataaagtaa aggtcttgtc aaaaggagaa acttatgcgt
     2461 aaggtaaaaa tgattgcatc tggtattgtc caaggagttg gttttcgatg gtctgtccaa
     2521 taccttgcta tggaaattgg tgatatagct ggtagggtct ggaacaacga agatggtact
     2581 gtaaccatcc tcgcccaatc agaaaatgct gagaagctca gccattttat tcatgaaata
     2641 cgaaaagggc cctctcgtat ggccaaagtg acctacctag atgttaccct ggccaatttt
     2701 gaagactata aggattttca ggttagctat agataaaact tgtatatttc cgtcaaaaaa
     2761 agtaaaatag agagtatact gaaaaaaaca aaggaacgaa aaaaattgaa aaataaacgt
     2821 ctattcatgt tgtcaggcat ggccttatca acacttcttt tcttatctgg ttgtgttcaa
     2881 actgataaaa gcggaaatcc tacaggtctc atttgggatt ggttgggaca accaatgtcc
     2941 aacttgattc gcttctttgc agaagatcaa gctctaggat ttggtcttgc tattatcatc
     3001 gtcaccttat tggttcgatt catcatcctc ccattgggaa tttaccagtc ttggaaagct
     3061 gctatccaat ctgagaagat gaattatctc aaaccgattt taggcccaat ccaggagcgc
     3121 atgaaaaatg caagctctca agaagagcaa cttgctgcac aacaagagta ttttgcaact
     3181 caaaaacaat acggtgtgag catgctcggc ggaattggtt gtttgccgat gctgattcaa
     3241 atgccattct tctctgcaat ctactttgcg gctcgccaca caccaggaat ttcagaagct
     3301 actttccttg gtattaattt aggttcaact agcctgattt tgacagttgt tgctggtatt
     3361 ttatactatg cccaatcgct cttaatgcaa gtcggcatgg atgaagaaca gaaaaaacaa
     3421 atgaaaacca tggctctgat gaacccaatt atgatcatga tgttctcatt tgggtctcct
     3481 gctggtgtga ctctctactg ggttgtcggt ggtctcttcg gtattcttca acaagctatt
     3541 acaaacttca ttttgaaacc tcgtattcgt aaacaagtgg aagaggaatt caaaaatatc
     3601 acagtgtcag caactcctcg taaaacgaag gacgttactc caactgcttc agcagttatt
     3661 gaagaaaaga catctaacaa gaagagaaac cgtaatgcag gaaaacaacg ttctagataa
     3721 taaacactaa aagattggaa tttccaatct tttttagcac cttcgtgaat aaaaagagag
     3781 aatctttcga ctctctccac accttatttc gttttttcaa ccttcaagat ttttacatca
     3841 taactaccag caggtgtttc aattgttgca acatctcctg tttttttacc aatcaaagca
     3901 tgaccaattg ggctttcatt tgaaatcttg tttgcaaagg catctgcacc tgcagcacca
     3961 acgatatgat agacttcttc ctcggtctca ccaacttctt gaacagtcac agtcttacca
     4021 attgctactt catccgcagc aactgcatca ctgtttacga tttcagcata gcgaattttt
     4081 gtttcgattg ttgagatttg accttcaaca aaagcctgtt catctttagc tgcttcgtac
     4141 tcagagtttt ctgaaaggtc tccgtaagaa cgagcaattt taatacgttc aacaatttct
     4201 ggtcgacgaa ctagctttag ttcctctaat tctttttcta gtttttcctt ttcttccaag
     4261 gtcattggat atgttttttc tgccatttgt ctttcctttt cttttatttt tcatgaaaac
     4321 aaaattatgt ggaaaaccac ataattttgt tttaagaatt tactcattat taagatgtgc
     4381 atttacatac ttagcaacgt tttggttatg ttcatcaata ttatttgtaa agtaaacatt
     4441 tccagttgta acatctgcta caaagtaaag atagtctgtt gtgttggcat taatcactgc
     4501 ctcgatagcc gaaaggcttg gactatccac tggtccaggc atcaatcctg gtgtccaata
     4561 aatgttgtat ggtgactcaa ttgatgtatc aatcgctgca tcttctgcta gagttgtttc
     4621 ttgaccaagt ttaccctgag catacaagat tgcaatatta gattgtagag gcattgctgc
     4681 attcaagcgg ttaaagaaga cacttgcaat gtttcgacga tcttcatcag tagaaccttc
     4741 tttttcaacc aaagatgcca aggtaagaac ttcatttaca gtcaaattct tagcagtaat
     4801 cgtttcataa tatggttgta aacgattatc catcgcggca atcatttgct caacgagctc
     4861 ttcgatagtt gtttcatcgc tgtattcata aaccgctggg aagaggtaac cttccaactg
     4921 ataaataact ccactatcag cagctggtaa gctggcaaac agttttggat aagtagcaac
     4981 cattcgatta ataaaatctt ggttggtcac agtagccata aactgttctg cagtaaatgg
     5041 tgttttatcg ttcttatctt ttgtattagt attgtctgta atagcctgag caatttgtgt
     5101 taatgtatac ccttcaacaa tcaaaacctt ccctgcagct tctttttgag ctgtcggagt
     5161 tccgctttct tgaagtgctt tggcgatgtc atccacagac atattttgtt ttaaattata
     5221 aaaaccactt tggaaattat tataactctt aattttagag tagtaattaa aaatagtagc
     5281 attcttaatc aacttgttgt caactaaaat tttaccaatt tctaaagttg aagttccttc
     5341 tggaatctct acctgaatag tttctgtagc ttttgcatcg actggttcca gactagattt
     5401 tacccacata taaccagtaa cacctgtcac tagaactgcc actacaacaa tcgacatgat
     5461 aacagagaca atgtgtttag cggcattatc ttgtttttta cgacgttgct tgcttgttct
     5521 gtgggttgtt cttcttctgg gttgtgtttc ctgcatctgc ttgtctactt ctggaacaat
     5581 agccgttgtc tttggagcaa taatagtttc ctctagtgag atgatttctg tatcaccagt
     5641 cacagctgac acgtcttccg ttggaaattc taaaaatgta tcttctaaag gagaatttcc
     5701 cggaaggaca gactctactg gctcagaaac tgacgagctt gatggtactt catcaaatcc
     5761 tgccgtatta gcagccacaa gattccttaa tttctccaag tttcgttcaa cagtatcttg
     5821 tggaactgga cttgaaacaa cttctttttc aatcacaaaa gtctctggag tagccaaagt
     5881 ttctgatgtt ggctcttcaa tactttccgt catcacttct gattcttgct gttcttctga
     5941 caagtatgaa tcagggaaag caataccttc tacttttaca atttcagtct ctctatgggg
     6001 ggctttttct tcggcaagac taggctggag ctcgttgata acttcattac gcttatcaac
     6061 aagaacatcc gctgactgtt ccgccaaacg tttcaccttc aattcttcca aatctcgtaa
     6121 aatttgatcg cggaagcctg aagactgcgt gtttttttca ttcgtatcct ttgtcacgct
     6181 gttactcctt tcacacagtt actatacatt atatcttaaa ggcttatgaa ttgcaagaac
     6241 ttccctttct acaaggcttt ccagtacata tcactccacc tttgtcctcc aaattgcgac
     6301 aaggaggggc caaaatcttc aaactggttc atttcatagt agctaatcaa atacccctta
     6361 caggtcaggg aaactccttc aaatcctccc gctaaagcca cttcttttat ggctgccaac
     6421 aaaagagttc ccactccctg tcccttatag gaatccgcta cggatagact ggcaatagct
     6481 aggtgactgc ctgtcggcat gtcactctct gtcaagtaaa aaatatcgtc tgtaacagtc
     6541 tgcgaaacag agggacagga taataaatat cctgctattt ccccatcgtg ttccattatt
     6601 aaacaggttt tactcaaagt attcagataa gtagcaagaa cagactcagc tatccgttcc
     6661 tctttagaaa aattggcatt ttcaatcaaa acaacttgtt ctaaatccga aggagtagca
     6721 aaacgaattt ccataatttt ctcccttata tagcggaaaa acaggggcca taggcctccg
     6781 tctttctttt tattggacgt tatttgtaag atttgacaag agtctttcga aggaatattc
     6841 ataggactga atatcaccag ctcccataaa tacatatact gcattgtcat ggtctaaaag
     6901 tggtgaagtg ttttcaactg taacaagtcc accttttttg ttgattttag ccgccaaatc
     6961 ttctacctta acctgaccat tgtctgtttc acgcgctgat ccataaattt gagcaaggta
     7021 aactgcatct gctccattca aagcatctgc aaactcatcg agaagcgcaa tggtacgagt
     7081 gaatgtatgt ggttggaaga tggctaccaa ctccttgcta ggatacttct gacgtgccgc
     7141 atcaatggtt gcaatgattt ccgttggatg gtgagcaaag tcatcaatga taacagtatc
     7201 attgacaatt ttctccgtaa agcgacgttt gacacctcca aatgtcttca agtgttctgc
     7261 aaccagttgc aaatcaaatc cagcaatata gagattggca ataacagctg ttgcattcat
     7321 cacattatgc ttaccaaagg ttggaatctg gaattctccc agttcttctt caccatggcg
     7381 aactttgaat tgtgaaccac ttgtagaagg cttcaagtca taggcaacaa aatcgttgtt
     7441 ttcctcaaaa ccatagtaat aaattggagc atttgccgtg atacgacgaa gttgctcatc
     7501 ttcaccaaaa acgaggagag ctttttttac ttgcttcgca taatcattga aagcattgaa
     7561 aacatcctct agactagtga aataatctgg atggtcaaaa tcaatattgg taatgatgct
     7621 gtattcagga tggtatgggg caaagtgacg ttcgtattca tcggattcga acacaaaata
     7681 ctgagcattt gccgaaccgc gaccagtacc atcaccaatc aagaaggaag tatctgtgat
     7741 attgcgcata acatgtgcca ataatcccgt tgtagaggtc ttaccatggg caccagcaac
     7801 accgaggctc gtaaaacctt tcataaattc acctagaaat tcatggtaac gtttgtaggt
     7861 atagccctgg gcatcaacat aagcgatttc cacattgtta tctggacgaa aagcattacc
     7921 agcaatcaat tcaacatctg ctgtaatatt tttctcatca aacggtaaaa tctgaatccc
     7981 agcttgttca agcccgcgtt gtgtaaagta gtatttctct acatcactac cttgaacctt
     8041 gtgccccatt tgatgcaaca taagagcaag tgcgctcata cctgatccct taattccgat
     8101 aaaatgatag gtttttgtca tgttattcct cttctatatg tgtcaaattc aattcttgtg
     8161 ccacttgtcg atctcgttgt tgtctgcgct caggataatt gtaaacctga ctttttctta
     8221 aaaagtcata cgaatttttc tttataacct gctcctgttc tctatcttct ttcttcaaag
     8281 aatagacagc tggcatatca gcgagaatat agtctgtctg acgtaagttg tccgccaaac
     8341 gtgtcaaccg acctgcctta gattcagttg ttgaaacgac aggaggtttc accacaactt
     8401 tagctggtgc tggtttttca tctcttaagt aggttgccga tcgttttttc ttcaagtctt
     8461 ctctagcctg ttcgcgagcc aactgcccct gtgttttcac tggagaaggc ttcgttttag
     8521 tcagttgctg aaggcgttcc tttcgagaat aatggcttgg cttagcttca aacagaggag
     8581 gcaggagctc atcttccgtt tgtgctactg gttttgccga cgcaactcgc tgtgattcgg
     8641 tagaccagct aaattccttt tcttgataaa gccccctgat attgctaatc aggtcgctct
     8701 catcatataa actcataatt ggattttctc caactagtac ctcatcatct gcgactagtg
     8761 gaaatctttt ttcatgtcta atcataagtc atcctttcga acccttatta taccatactt
     8821 aggccttatt tcctaattct acgctgagtc aactgattcc atatcactca atccctaaaa
     8881 tctcacgaat atcctcaacc gtcaaattcg ctcggctttc attgccatca aggactgttt
     8941 tgacaaggtt ctttttattt tcttgcaatt cttgaatctt ctcttcgatt gtccctctag
     9001 taatcaggcg atagcattca accttttcag tctgccccat gcggtgagcc cggctgatag
     9061 cctgtgcttc cacggcagga ttccaccaaa ggtcgactag gatgactgtg tctgcacctg
     9121 tcaaattcaa gccaacgcca cctgctttca aggaaatgag aaaggcatct cgacttcctg
     9181 cattgaaggc ctgtgtcatt tcttggcgtt ggttagcagg tgttgaacca gtcagagtat
     9241 aggatgtcat cccaatttct accagctgtt cttctatttt ttccagcata tttctgaatt
     9301 gggagaatat caagaccctg tgttcacctt cttttagctg caagagtaat tctttcaaac
     9361 tattaagttt gccactctca ccactaaact cttccatgaa aaggctgggg gtatcgcaga
     9421 tttgacgtaa gcgagtaatt cctgatagga tttctatctt gctacgattg atttgagcat
     9481 catctgcgcc agctatttga gtccgcattt gttgaagctg ggctaggtag atggcttttt
     9541 gttcatctgt cagttcatta agtacattga cctctatcaa atcaggcaat tcctgaagaa
     9601 cgtcttcctt atgacgtctg agaacgaagg gttgaatggt ccgcgcaatg tccttggctg
     9661 ctaatttacc gaagtcttgt ttgcctggca ataaacctgg taagacaatt tggaaaatgg
     9721 accagagttc cgtcaaatgg ttttcaatcg gcgtacctga gagggcaaag caattcccaa
     9781 cctcaaactc acgcagtagc tgtgcaatct tagattgggc attcttcatg acctgcgcct
     9841 catctaaaat gaggtagtca aagcgttcct gtttatagag tgcaacatcc tgtctgaagg
     9901 atggatagga ggtaactaag acttggtgac cattggcaat gatctcttca cgctgttcct
     9961 tgtttccatg gacaacagcc acatctaaac ttggagcaaa ccgcttacac tcatctaacc
    10021 agttataaat caagctagac ggtgccaata ttaaaacttt ggaatctttt gtcatccgac
    10081 tggacagaaa ggcgatagtt tgcaaggtct ttccaagccc catgtcgtca gctaaaattc
    10141 cgccaaaacc atacgtatcc agcatagtca accacttaag ccctgtctgc tgataatccc
    10201 taagcgacgt tgttacttcc acagcaggaa gcgggaacaa atctggttga gctagatagc
    10261 ttgccatttc ctcaaattcc ttggagaaac tgacttggtt gaattgtgac aaggattgag
    10321 ccaattggta accggcaaga gcatggactt gtacctgccc ctccttacca tgacgggccc
    10381 gcaaatagat tagggtttga ctgattttct tggtttcctc atcaaaaaca aataccttac
    10441 cgctcgggct agtatagtgg tcctcttgat tcagcaaggc tgtaatcgcc tgatcaattt
    10501 cagattgttc aatacctgat aagtcaaacg aaatatccaa aagtgagccg ttacgcacaa
    10561 tttctaattg aggtttggct tcaatatata aagcctgtag cctttctgct agatgaacct
    10621 gccccatttt ttgtaatata ggcaattgtt gttggaaaaa tggatagagc tcctgctgac
    10681 taagagccgc tctctgggca tgatatgtcc ccctaaagcc agctaaacga atagcctgat
    10741 agaccctctc caaatgttgg aaatggctgg caaaaggcag gagagccaaa tcctcttcac
    10801 tccttacctt acgtccatca aaatccaaaa ccaattggag ggataaactt tcatttgcct
    10861 ccatctgaat atgaaattct ggcttgaaat catgaatgat gaagcgttta ggtgccttaa
    10921 cagtcccaat tgtctgcaaa tccagcaagc ttaaagccag acgattttgg tcagggaaat
    10981 ccacctgcac ttttctcaaa cctgattcgg ttggaacaag ttcggaaatt tggtaaatca
    11041 gatgttcttg atggcgattg acactataaa ccgtgccatt tactaagaga tagcgcccat
    11101 gaaaaagagg tcggactgtt ttttcgtgaa taaccatttc aatcatctgc gtgtgaacgg
    11161 tcacctcaaa atgatagagc ccttcctccc ctgtcaacgg aagaaccttc aaggaggaat
    11221 aggactgctg ttgataggaa aagttaaaat gttctagctg atttaacagc tctaacccct
    11281 cctcaaaata ggcttgtggc agacgaaaat gtcgtccaaa atgagtaaga atatccgaat
    11341 ctataccaac tttctctggt accaaggccc agagaaaatc aaggagagcc tgacttggtt
    11401 catcaaaatt ttcataagta acttgctcat aataattttt acctatttga tagtgaccgt
    11461 tggctttcac aatgcgcaaa aaagctccga tgtcccgcac aatgtacgaa cgtgtatctg
    11521 gcaaacgggt aattttcaag gtccaatcta tctgacgatc atagggcaat aagctccctt
    11581 caactgacaa ctgatactta agccccagtt caggttctgg ccgcaaaatt tcatctaaaa
    11641 agagaccgcc aaaataagtc cgacggacgg tttccttatg ttgttcgcta tcttctttca
    11701 aagactgctt catatccttg cctgaggcat cattttttaa aaaatactcc gtggcagcta
    11761 ggtgttgaca gtagccctta ctttgaaata actggcaact acactgtaaa tcgctatctg
    11821 catcgccgta agtaaagcgc tcaccagcaa tatccagcac catctttcca ttctcactat
    11881 gaagaacagt cagttttcca gcctcgtaca aatcaattcc ttcttggcga atccgccctg
    11941 gcatcatgcg acccattcta actcctttat ccttcaacca atcatcaact cttctgtaaa
    12001 atatatctaa aatattatct tttcattata gcatattttc aatttgaatt gtttaaatag
    12061 gtatcaaaaa aacaccagca ggtaaactgg tgcctctaat gaatagctaa ataataaatc
    12121 gttgcttgcc acattttcaa gattgttttt ccgcttttcc aaagccaaaa tagggcaaat
    12181 agcaggaaaa caactgccac tactacgtta atcaagcctt ttccaaccaa gaaaaacttg
    12241 aaaaagaaga cagtcacaca agtgagcaag gcaaggagta agacatgaat cagaactccc
    12301 aaccagccca gccaatagcg aattccaaag ggagggtgtt tcttttcatc gaagtctgct
    12361 agaccagtca aaaactcaac tagcaactca ccaaaaaatt ccatactagc ctacctttct
    12421 tacctgtcga taatatttgg ttcgtcgaat catcatcttg atagccttaa aggaaagtaa
    12481 ggttagcata ctagccacaa atagacaaaa aaggaatatc acaaccaatg gctgtttata
    12541 ctgattatag accataattg aaccaccagt aaaggcgata aaataggatg ccaaagtcac
    12601 aaaattgagc cagatacaag ccactagccc cagccaataa cgaaatccga aaggcttata
    12661 ctgactgtct tcataaaata caagaaaaaa tgatgaaaaa gaaagcaata tcaaaaaaga
    12721 acgccaaatc ggaaccgcta ccataccaac acctccaagt ccattctagc atatttacga
    12781 ataggtgcaa agaaaaaaga ggtttccctc tttacttccg tttccgtgca atcagatgaa
    12841 tcggtgtccc ttcaaataca aaggccttgc ggatttgatt ttccaagaaa cgcatatagg
    12901 agaagtgcat gagctcttct tcgttgacaa agaccacaaa ggtcggtggt ttggtcgcaa
    12961 cctgagtcgc atagaaaatc ttgagacgtt tacccttgtc agttggtgtc gggttaatgg
    13021 caatggcatc catgataaca tcgttgagaa cagccgatgg aatacgggta ttctggcttt
    13081 cactgatggc cttaatcatc tcaggcaact tgtgcaagcg ttgcttggtc aaggctgata
    13141 caaagataat cggtgcgtaa gacaagtatt ggaactggtc gcggatatca tcttcccatt
    13201 tcttcatggt gtggttgtct ttttccaacg tgtcccactt gttgaccacg ataatcattc
    13261 ccttaccagc ttcatgggca aaacctgcga tgcgcttgtc atactcacga ataccttctt
    13321 ctgcattgat gaccatgagt ataacatccg agcgttcaat ggcacgcatg gcacgcataa
    13381 cagagtattt ctccgtattt tcatagacct taccagactt gcgcatccct gcggtatcaa
    13441 tcatggtaaa ttcctgacct tctgggtctg taaagtgcgt atcaatggca tcacgagtcg
    13501 ttccagcaac aggcgacgca atcacgcgct cttcacccaa aatagcgtta atcaaacttg
    13561 attttccaac gtttggacga ccaatcaagc tgaacttgat aatatctgga ttttcttctg
    13621 cttcttggac tggcagattt tcaatgatag cgtccaagac atcccctgta ccgataccat
    13681 gcacagatga tactgggtag ggatccccaa gaccaagcga atagaaatca tagatgtcat
    13741 tacgcatttc agggttatcg accttgttga caaccaaaat gactggctta ttggtcttgt
    13801 aaaggatacg cgatacatac tcgtcagcat ccgtcacacc ttccttgcct gatacgacaa
    13861 agacgataac gtccgcttca tccatggcaa tctcagcctg atgcttgatt tgctccatga
    13921 aaggtgcatc cacatcatca ataccgcctg tatcaatcaa ggaaaactta cggttgagcc
    13981 actcaccagt agcataaata cggtcgcggg tcacaccttc cacgtcttct acgatagaaa
    14041 tacgttcccc cgcaatacgg ttaaatagtg ttgatttacc aacgttggga cggccaacga
    14101 tggcgatagt tggtagagcc atagctcctc ctcttctagt ttacaataat ttattctttt
    14161 ctaagataga ctttgtttct ggttgttcag cctgaccaaa ctgggcaacc attcgctcag
    14221 accaagtttc tgtctgacgg gcacctgcat aggctgtctg aacttggtcg taggcttgga
    14281 ttacttccac gccttgctcc acatattctt cctgaaagac aatggtttca cttgccaaac
    14341 gtggtttaac aggatggttt tgggctggtc gacccaaggc aatacagaaa attggatagg
    14401 tataatctgg caagttgaac agctctgcta ttgccatggc cttatggcga atcataccga
    14461 taaagacgcc accgtaacct aggctttcag ctgctaaaag ggcattttgc cctgctaggc
    14521 tcgcatcaac tgaagaaatc aggagattct ctgttccttt ggcatcaaag tctgaaccat
    14581 ggagttgagc cgccttgctg gcacgattag ggtcgcccac aaaaaccaga atggcctgag
    14641 cctgtaaaat agctggctgg ggcaccaaat catagagtgc ctgttttttc tcctccaact
    14701 gcaccacaat gatggaataa gactggaaat tcttccagct agacgcagct cgcccagctg
    14761 aaataatagc ttgcaaatgt tccgcctcaa taggctcctc cgtaaaacga cggacggaag
    14821 tatgattcag catcaagtct atggtttcat tcatcggcgg ttatttcctt tcaaatgcaa
    14881 atcgcgagcc agatagcgaa tccgctccat aacccgtttg gcctgccagg tctcatcacc
    14941 agacttgcca gaggcgaaat gacgctccaa atcatcaaag gagaaattgg aggtaaaaaa
    15001 ggtcggcaga ttttcctgca tgcggtattg aagaatgact tgcaggacat cgtcacgtac
    15061 ccaaggacta tgttgctccg ctccgatgtc atctagcacc aagacctgag ccatcttgat
    15121 ttcatcaatt cgctccttga cagaaccatc tttgatggca ttttttatat cgacaacaaa
    15181 ggttggataa tgaagcatag tggttgattg aagatgggtc ttggacaaga gattggctaa
    15241 ataagccatc aaataacttt ttccaatacc gaaattgcca tagatgtaga gcccttttgg
    15301 tttttcctca aatcgtttga caaaatctgc aatagccagc atgacttcca cacgattttc
    15361 atcactctta tcaatatccg ccactgttac attcttcaaa ctcgcaggca tattgatgag
    15421 ctggatacga ttcttgatgg cttccaaacg acggtattcg accaattcct ctgtctcaag
    15481 ataggacaca tccgcatagc cctcgttcat gactaaaacc ggttgatagc cttttgcaat
    15541 ataggcttca tcctgcttga caaagaggtc acgctgactg atatattcca aaaacttaga
    15601 aatggagagg gtgatttcct gctgggtcag accttctttt ttgataaaag cagcgacttc
    15661 aggatcactc actatctcct gatagagttg ctgataagat tttggacttg gattggttgt
    15721 ttgcgacaac ctgtcttgaa ctgatttcat cagctacctc ctttgttttc catttcttct
    15781 aattcacgat aaagagccgc cagcttggct tgaccttcct gcgtttgtcc ttgctggact
    15841 tcttccttac tccactctgg cacattggtt ttctgtggct ggctcgactt ggtaacaact
    15901 tgacctgtct tcaactctct taggtaaaga acagctgcct ctgcactgac acttcccttg
    15961 taggaaaaat cattgcccag cttcatagcg tatttttcat ttagattggc tgagtccacc
    16021 ttgttaaagg tatagaggac caagacattg atgacttcgt caagcagacc caaattggcc
    16081 aaatccgtca agcattttcg ttctgttcct gtcacagctg ccttgcgttg ctccttgata
    16141 aaggacagga aggttaggct agattttgat ttcgcttcac gaacaatggc ttgttcttgg
    16201 gcagtgaggt tggcgttttc tgttggcaat gactgacgtg actgttgcaa gcgtttggtc
    16261 gagatggtct gtccaatagc tgttgcttta gcctggcgat aggtttccaa ccaggtccac
    16321 cccgcctgct ctgctatgta actaagcgca ataatatcct cagcttcatc ctgaaaaaga
    16381 agcttatctt tagccattaa tgccttgaag gcagaccagt caaaatcatg tttcggttca
    16441 aaaacaactg gagcctgccc atccatggta aagacctgag aaaatgtttt tgagatattc
    16501 tgttcttgat tgggttggtg aacaagcaga ttctccatgc ttgactcccc aatcttcctt
    16561 gccaacaagt tcttatacaa gggtttcgct agaaaacttt ttacggtcag aggggcttgt
    16621 agcgccaaac ctgttaattc atctgcacga tagagttcta ggagccccat ggcagtcaga
    16681 acatccagag ccttctccaa acgattcatc ccaaaatcca tgtgattgag aatgacagtc
    16741 catttgtaac gtccttgccc ttgatcggca aaactataaa agtaataata aagcgctaat
    16801 gcatcaaaac cgataatcgg ctggtagcat tggctaagac tgacaatatc aggtgtaaac
    16861 gggctgattt ttatataggc aaataaatca tttggtttca tctttttttc ccggttttac
    16921 cttacttcct ttggaaatca tctgtttaag caaattttct agctctccaa catctttgaa
    16981 agaacgataa accgatgcaa aacgcacata ggtgatttca tccaattcga ccaattcttc
    17041 cattaccaaa ttcccaatga catcggattc tatttcacta tcaccttgtg ctcgaacctt
    17101 ctgttcaatc cgattcacca cttcatcaat atcatcggtt gacacagggc gcttttgagc
    17161 agaacgaata atcccattga aaatcttttc acgtgaaaac tgctctctag ttccgtcttt
    17221 tttcacaacg actagagttt tctcttcaat ccgttcatag gtggtaaaac gttgaccaca
    17281 ttggtcgcag gaacgacgac gacggatggt attgccatct tcagcctgac gactatcaat
    17341 gacacttgat ttcaaactct gacattttgg acaacgcata ctctttctcc tacataaact
    17401 ttatcacttc agtatagcat aaaaatgaca aaaataaaag aaagcgagaa ttactcactt
    17461 tctgccaata attcaccttg cttcttcaat cgactggcaa cccattcacg aaccaagcga
    17521 tagacaaccg caaaaattgg ggtaaagaag accataccaa gtaggccaaa caaattacca
    17581 ccaatcaagg ctgctgctag ggtaaagaga gtcggaagac caactgattg accaactacg
    17641 cgcgggtaaa tcacattccc ttcaatcaac tgaataatct ggaaaagggc gatagaccag
    17701 agtgccaaga gcggatcttg taccgccaca aaaagagcac taatcaaaca cgctgaaaag
    17761 ggaccgatat aaggtacaaa tgataggacc ccagccaaaa taccagccat gctggcataa
    17821 ggaattcctg agagcgaata actaacaaag acaagtgtcc caatgataca cgcctcaaca
    17881 atctgactca tcaagaatcg gtcataagta tcaacaataa cttctccaat atagttcact
    17941 actctgacag ccttctctgg caaggtagcc tgcaagaact ttctcgtcat cgcctgcaag
    18001 tgttccttgc tccctaaaat cgctagagtg aagaagaaag ccattatcaa cgtcatagtg
    18061 ttcgaaaaaa tccccgtcac attggacacc aagccagata aaatcggtgt gaccaaactt
    18121 gaaataagtg ttaaattctt caactgatcc aaaaagctgg caatctggtc tccgatttgt
    18181 cccgacaaaa gaccatttga ttcaagaaaa ttagttaggg catttaccga ttttggaata
    18241 gctgtactac taaccgaaac cagttctgaa acagtcgtca ccaatgtagg aagaacaatc
    18301 accaccagtc ctgtcacaat taaagcaaat ccaattaata ctccaacaat tgccaaagac
    18361 cgcttggatt tctttaaaaa agaaattttt tcaaactgat cttctaattt tttcatggga
    18421 acattgagga tgaaggcaaa aatagctcca ataaaaagcg aattcatact acctaataat
    18481 tgttgaatag ctccataaat actagaccag ttgagaacag ctaacaatgt cgctccagat
    18541 aagagtatca atatgacttt ttctttaaat gatgtattca taacttttcc attcttactt
    18601 attggctatt attttatcac acgcttatag aaaaagaaag agggaaagct tccctcctct
    18661 ctactctaaa tcaatcgcgc atggcatatc cgacaccacg aactgttttt atatatgaat
    18721 cttgatgtgg caaatccagt ttaccacgta gatagcgaat atatacatcg acgacattgg
    18781 tctcagaagc cgcttcatat ttccaaacac gctcaagaag ttgttctcga ctaacaggct
    18841 ctgggctatt cattaaggtc gccaacagat catactctcg gcgggtcaaa ttaatcaatt
    18901 catcacctcg agtcactgtt cgattttgaa aatcaacctt caaatcacga taggccgcat
    18961 gcatgtgaac ctgtttacag ttattgtcaa taaaatcacg accacgaaaa atcgcagaga
    19021 tttgctccac taaatcactg atgacaaagg gcttcactac gtaggaaaca gcataagaga
    19081 ggacttcttc cccatattga ctaacttctg tcggctcaac taccacaatc atgaccgtgg
    19141 ctggttttat cgctaatagt tctgcagcca attccttgct ggacatgtct gaaagctgaa
    19201 aactcatcag aatcaagtca aaatccgttt catgtgccaa ggacatagcc tctttccctg
    19261 tcgatgcata atcaacaaga tagtcttttt tctgcaattc catggaaaca aaatgcgaga
    19321 gattgcgttc tttaccagca atcaaaattt tcttagccat tgtccatcct tttctattct
    19381 ttcaaatggt gtaggaatcg tattatgact agaaagaagg gcgcttccac gcccatcttt
    19441 tctattttga ttcgctatac cagtcgtaat ggaagattcc ttctttatct ttacggttat
    19501 aagtgtgagc accaaagtag tcacgttgtg cttgaatcaa gttcgctggc aagttttcag
    19561 cacggtagct atcgaagtag gtaatagctg ctgagaatgt tggtacagga actcctgctt
    19621 gaactgccaa ggcaacaaca tcacgaactg cctgttgata cttagctgtt acatcaagga
    19681 agtactcatc caagagcaag tttgccaagt cttcatcacg tccgtaggca tctgtgattt
    19741 tttgcaagaa gcgagcgcgg atgatacaac ctgcacgcca gatttttgcg atttcaccaa
    19801 atggcaagtt ccagttgttt tcttttgatg ctacacgcaa ctgtgcaaaa ccttgtgcat
    19861 aagacatgat ttttgagaag tacaatgctt gacggatttt ttctaccaat tcagccttgt
    19921 cgccttcgta tgcaaacgga gctggttttg gaaggacctt gcttgctgct acacgctcat
    19981 ctttgtaggt tgagatataa cgagcaaata cagattctgt aatcaatgac aatggcacac
    20041 ccaagtcaag tgatgattgg cttgtccatt taccagttcc cttgttacca gccgcatcca
    20101 aaatgtagtc aacgattggt ccatcttgac cttgatcgtc tttacgtttc aaaatatctg
    20161 ctgtgatttc aatcaagtag ctatccaatt cgcctttgtt ccactcagta aagatgtcag
    20221 ccatttcatc cactgacaaa ccgagcaagt gttgcatgag gtcataagac tccgcaatca
    20281 attgcatgtc gccatactcg ataccgttgt gtaccatttt tacgtagtga ccagctccat
    20341 ctggaccgat ataagtcaca catggtgctc catcttctgg tgcttttgct gaaatttctt
    20401 ccaagacatc tgccaccaat tcgtaagctt ctttttgacc accaggcatg atagatggac
    20461 cttcaagggc tcctttttca ccaccagata cacctgtacc gatgaagtta atacctgagt
    20521 ttgccaattc ttttgaacga cggatagtgt cttcgtagaa agtattacca ccatcgatca
    20581 agatgtcacc ttcatccaag tgtggcaaga gcgcttgaat ggttgcatct gtaccaggac
    20641 cagcttgaac cataagcatg atgcgacgtg gtttttcgat agatgccaca aagctttcca
    20701 catcgtaact tggtaccaag tttttacctg ggttgcttgc aacaacgtct tctgttttat
    20761 cagcagagcg gttgtaaata gctactgagt agccacgtga ttcaacgtta agtgcaaggt
    20821 tgcggcccat aacggccatc cctacaacac caaaatttgc tttagtcatt tgtttactcc
    20881 tctaatttgt atctctttta ttatacaccg aaagctgaaa attgacaaga attttcagat
    20941 aatttaacta tcttgccaat ctgagttagt cctcatctcc aaataaccct tgtaattggg
    21001 caaaaggact attggcctct tttttctctt gagctttttg ttgaaaatct tcttcagtca
    21061 ttaaagccca agcttgacca gatggtagtt cctgtccagc ttcttcttca ggtgtcaaga
    21121 ctttgattgg tatagccagt aaaatattat ctgctacact ctcatcaagg acaatgtggt
    21181 catcttctac taccagtacc atatcctcat caatcaaatc ttgttcttta agaactgctt
    21241 cattggcaac aaagagttca ttgacgtttt ggacctcatg caaaacaact ggttgcagag
    21301 aacgactgga tgccaaggta atatcataag tcatctgata gtccaagaaa aagaaacctg
    21361 attcaaaacg gatatttcca gtgacttcga ctggcgacaa gcctagaact tcaccatttc
    21421 gagcttgcaa ttcttcctgt aaatccaagg ttttatcaaa ggagatgcca tctagatttt
    21481 tctgaatatc gtaaatatga aacattgtat tcccctattc agctagttct gtgatgtagt
    21541 tatagacttc ttgtccagca actgcaccat ttcctactgc cgttgcaatc tgacgaagtt
    21601 gattttgacg aatatcaccg atggcataga tgcctgattg agtcgtctcc atcttttcat
    21661 tagtgatgac ccaaccagct tcgtctgtaa ttcctaggtc cgcaactgta tcagtcatcg
    21721 gatccagacc gacatagata aagacaccac cgaaatcaag ttcactgact tcttcagttt
    21781 tcacattttt gataaccacg ctttgtacac gtaagtcatc gcccttgatt tcttcgacta
    21841 cgctatccca gataaagttt attttttcat tggcaaaggc gcgatcttgg atgacttttt
    21901 gagcacgaag ttgatcacgg cggtgaacaa ttgtcacaga ctccgcaaat tgtgtcaaga
    21961 agagagcttc ttcgaccgca gaatctccac caccgacaac caagagtttt tgtccacgga
    22021 agaaggcacc gtcacagact gcacaatatg agacaccacg gctattgtaa gtatcctcac
    22081 ctggaatacc taacaaacgg tgttttgccc ccatggctag aacgactgtc tttgtctcca
    22141 aaacaccatc ctcagtaatc actttcttaa tttgtccttc ttcctcaata cggactaggg
    22201 tgccaaaaat atggtccaca ccgaattttt ccaagggttc aaacattttt tcagccaagg
    22261 ctggacctga aatatggtca tagcctggat agttttcaat ttccgcggta ttgttcatct
    22321 gaccaccata aataccacgt tccaaaagag ctacctttaa attgctacgc cctgcataaa
    22381 gagctgcagt catccccgca ggacccgcac caattacaac tgtatcgtac atatactttt
    22441 tccttctacg ctttaaacat taataagatt cccactactc caaaggctgc aatgggaatt
    22501 gtcatcacat taatcatcaa caaatcaaac aagaaatctt gtcgttcttg caaggtttta
    22561 tccacaccct ttgtcgcacg ccagataaac cagaccagac tagaagtgat aatcaacaag
    22621 gaagctatta gcaatccttg taaataaaaa cttagaatgt tttgtaacat ctttttcctc
    22681 gattacttta aaaggtttga cgcttggctt tacgttctgc acgacccttt gcacgcgctt
    22741 caactcgctt ggtcttgcga cgtttttcat caacagccca ttgaattttc ttcttgtagc
    22801 ctggtttgac ttttttcttt ttcttcttga ccaagccaat catttccaaa tctagttttt
    22861 cacgagattt ttcacggtta gcacggcggt cacggtcgta ggtatcgaca aattcgccat
    22921 ttttcatttc ttttggcaag aatttgatat tgagtttttc tagctctcga atatctgcat
    22981 catcgcttgg ctgataaagg gtaatggcaa tgcctgataa accattacga cctgtgcgcc
    23041 ccacacggtg aacaaagaag gaaagatcct gtggaatggc atcattgatg acatgactga
    23101 cgccctcaat atcaatccca cgtgctgcta aatccgttgc aacaatgtac tgatagtcca
    23161 agttcttcac ctggttcatc atccgcttgc gctcacgtgg aggaatatcc ccatgaatct
    23221 ttgcgacacg aagaccgttc gcagtcaaat agctatgcaa ttcatctgca cgtgtctttg
    23281 tattgacaaa aatcatggcc aagtaaggtt gcatgagctt ggttacttcc aagatttgag
    23341 cattacggtc acgccccttg gttgaaatca gccagttttc aatggtatct gaaatgaccg
    23401 tatgggtctt gatttgttcc atgactggat tggacaggta ttttttcaaa aacggttgga
    23461 gtttctgcgg aatggtcgct gaaaagacca taaattgcaa gtcttttggc aggctgccag
    23521 caatcttatc aactgtttcc aaaaagccca tgtccaaggt catatctgcc tcatcaacca
    23581 caaaggtttt ggctttatgg atagcaaggt cacctgattt gaccaaatca tagatacgcc
    23641 ctggtgtccc aacaacaatg tgaggctgac tagcttgaag cttgtcaatt tgacggttct
    23701 tatccgtacc gccaacataa ttggtcacac gaacttcgat gtccgaatga cttgccaact
    23761 ggcgtgccgc ttgataaatc tgagtagcta attcacgaga tggggctgta ataacagcct
    23821 gaacttggtc caattcctca cttaattttt ggaaaatagg gatcaagaag gtatgggtct
    23881 tacccgaacc tgtttttgat tcccctatca agtctcgccc tgacaaaaca acaggaatca
    23941 atttctcctg aacttctgtc ggctggacaa agttaatttc tttcaaagcc tcctgaatat
    24001 aaggcttgag tttcatatct gtaaatttca tagtttcctc tttagttgta tttccctcta
    24061 ttatagcacg atttaggtgg acaagcaaaa aggacggata cccgtcctaa tcaactctcg
    24121 cttgcttcag taaattctgc ctcaataatt ccaagttcct tctcttcttc caaacttgct
    24181 ttaaaggcct cagtgagttc taattcgcca tcaaaatcac catttatttt tttcacaaat
    24241 gtatctgcca acatccctcc catgggctgg aaggtaaaat aggtcaaacc tcctgaaatc
    24301 agaccaccca aaataggaac tgcattggca atggtctttt caacagtctt tttggtaatt
    24361 ttcacaccaa tcactgcggc tacctttttc accaagggat gggag
//
LOCUS       DASFSW010000013        24035 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.013, whole genome shotgun sequence.
ACCESSION   DASFSW010000013 DASFSW010000000
VERSION     DASFSW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24035
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.013"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..75
                     /gene="dltX"
                     /locus_tag="U1099_000448"
     CDS             <1..75
                     /gene="dltX"
                     /locus_tag="U1099_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEM4866796.1"
                     /translation="ILLLLLYLYSYLDIGQGGFIYNEF"
     gene            92..1627
                     /gene="dltA"
                     /locus_tag="U1099_000449"
     CDS             92..1627
                     /gene="dltA"
                     /locus_tag="U1099_000449"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEM4866797.1"
                     /translation="MISSMLERVEHFATENPDYPVYHYLGQLYSYGDLKADSDSLAAH
                     IDSLDLPANSPVLVFGGQEYQMLAIFVALTKAGHPYIPVDSHSALDRIEAILEIAEPS
                     LVFAVADFPLETSLPVLGLSEVEAIFEKKSTYQLMHPVSGDDTYYIIFTSGTTGKPKG
                     VQISHDNLLSFTNWMIRDKEFALPERPQMLAQPSYSFDLSVMYWAPTLALGGTLFALP
                     KELTLDFKTLFAAIQTLPFKIWTSTPSFVDMALLSREFDGEHLPDLTHFYFDGEELTV
                     KTAQKLRERFPKAVIVNAYGPTEATVALSAVAITDEMLVNYKRLPIGYAKPDSPTFIL
                     DEDGQVVPNGQQGEIVVSGPAVSKGYLNNPERTEAAFFQFDGLQAYHTGDLGVMTDEG
                     LLLYGGRMDFQIKYNGYRIELEEVAQHLNKSTYIEAAVAVPRYNADHKVQNLLAYVVP
                     KAGIMEQFDRKLELTKVIKQELKERMMDYMMPSKFIYRDSLPLTANGKIDIKGLIAEV
                     NAQ"
     gene            1624..2865
                     /gene="dltB"
                     /locus_tag="U1099_000450"
     CDS             1624..2865
                     /gene="dltB"
                     /locus_tag="U1099_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEM4866798.1"
                     /translation="MMEFFKQLPQLEPYGNPLYFFYLVLALVPIFIGLFFKKRFPLYE
                     TGVSLAFIVLMFTGTKTMQLLSLLAYIIWQTMLIFFYKHYRQRANQSWVFYLIVCLAV
                     FPLTWVKLAPTFSQHGAIFGFLGISYLTFRSVGMVIEMRDGLLTEFSLGSFLRFLLFM
                     PTISSGPIDRYRRFTEDYKQIPERTELLNMLDQTMHYIMMGFLYKFILAYFIGHTLLE
                     PLKAVALDQGGWFNLPTIGVMYLYGFELFFDFAGYSMFAIGISNLMGIRCPINFDQPF
                     KSRDLKEFWNRWHISLSFWFRDFVFMRLVKTLLKHKVFKNRNTVSNVAYLLNMLLMGL
                     WHGVTWYYIAYGLFHALGLIINDAWIRKKKSINLARKKAGQEPLPDNRWTSFAGMFVT
                     FHTVMFSFLIFSGFLDKLWFK"
     gene            2908..3147
                     /gene="dltC"
                     /locus_tag="U1099_000451"
     CDS             2908..3147
                     /gene="dltC"
                     /locus_tag="U1099_000451"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEM4866799.1"
                     /translation="MDVKETILNIIEELFMEDVSEMMDEDLFDAGVLDSMGTVELIVE
                     LESRFNITVPVSEFGREDWNTANKIISGVVELMHA"
     gene            3140..4405
                     /gene="dltD"
                     /locus_tag="U1099_000452"
     CDS             3140..4405
                     /gene="dltD"
                     /locus_tag="U1099_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEM4866800.1"
                     /translation="MRKQLWQIFGPVICATFLVILTLLFFPSSSRSNFKEEKQDAVAL
                     SYISFKSKYKKNRALGDKNHRFVPFFGSSEWSRFDSMHPSVLADAYQREYTPYLLGQR
                     GAALLTQYFGMQQIGAHLKNSQAIYVVSPQWFSPKGASSKAFEQYFSSDQATHFLQTS
                     AGDDYDRYAAKRFLELKSDCSLTGMFQKVASGKELSKWDREQLTWQAYLLEKQDALFS
                     RFADSGSNLGLVETEAMKLPETFSYDRLSKVANEDGSRSTQSNDFQIDDRFYRNRLQS
                     KIKKLKQSQTKISYLQSPEYNDFQLVLNEFAKHQTDVLFVIPPVNERWMNYTGLNKDM
                     YRATVAKIKYQLTSQGFNHIADLSEQGGIDYFMQDTIHIGWQGWLELDKYIHPFLTEQ
                     QAPVHYQLKKEFLTSEWATYKGDLNLFLK"
     gene            complement(4494..5588)
                     /locus_tag="U1099_000453"
                     /pseudo
     CDS             complement(4494..5588)
                     /locus_tag="U1099_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
     gene            complement(5662..6618)
                     /locus_tag="U1099_000454"
     CDS             complement(5662..6618)
                     /locus_tag="U1099_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEM4866801.1"
                     /translation="MGLLQDGKWVDQWYDTKSTGGKFIRSVTQFRNWITPDGQAGPTG
                     EGGFKAESGRYHLYISLACPWASRTLIMRKLKGLEDHISLSVVHPLMLENGWTFEDGP
                     GVIKDTLFNSDYLYQVYLKADSHYSGRVTVPVLWDKETNTIVSNESAEIMRMFNSAFN
                     DITGNYDDYYPANLQAEIDAMNDFIYPNINNGVYKAGFSTSQAVYEKEVKNLFAALDK
                     LEEHLADKDYLVGNQLTEADIRLFTTLVRFDPVYFGHFKCNIKALVDYPNLWDYTKRI
                     YNHSGIAETVDFDHIKQHYYGSHKTINPTGIVPVGPDLDWTL"
     gene            6757..7473
                     /locus_tag="U1099_000455"
     CDS             6757..7473
                     /locus_tag="U1099_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM4866802.1"
                     /translation="MRLDKCLEKAKVGSRKQVKKLFKAQQIKINGQAAQSLSQIVDPE
                     LQTIQVSGKKVALEGSAYYLLHKPAGVVSAVTDQEHQTVIDLISPQDSREGLYPVGRL
                     DRDTEGLVLITNNGPLGYRMLHPSHHVDKFYYVEVNGCLAEDASKFFASGVTFLDGTR
                     CQPAVLTVLEASLDHSRATIKLAEGKFHQVKKMFLAYGVKVTYLKRISFGGFELGDLE
                     RGAYRQLTPNEMEHLFTYFD"
     gene            7487..7966
                     /locus_tag="U1099_000456"
     CDS             7487..7966
                     /locus_tag="U1099_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEM4866803.1"
                     /translation="MSIYDFTVQKQDGTDQSIAEYQGQVLLIVNTAPGCGLAPQYKEL
                     QELYDSYRGQGFVVLDFPCNQFLNQAPGSAEDINQTCSLNYGTTFPRFAKIAVNGSEA
                     SPLYRYLKKEKSTLLGGRIEWNFTKFLVDRQGRVVKRYLPTTSPLKLKEDIEFYLEK"
     gene            complement(7985..8986)
                     /locus_tag="U1099_000457"
     CDS             complement(7985..8986)
                     /locus_tag="U1099_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM4866804.1"
                     /translation="MIRHHNYLKIFTFLLILLFVIFLLSLSIGYSNSSFSDLIDIMSG
                     RASSSTLLIIGRIRLPRIIASIIGGASLALAGLLLQTLTRNPLADSGILGINAGAGLV
                     VALFVGLSSKISPVLLSLMPLFAMMGGGLTIFLVYWIARKKLYGIHPARLIITGVGIS
                     SMLSGIMVSIISQLDDFKMEYIVQWLSGRVNGGDWKTIAIYTPLLLLVWMATFSRSRS
                     LNIMNLNDQTAMALGLHLQRERRITLILATALASLSVILVGNITFVGLIAGHIVRKWL
                     GNDHRVIIPATMIIGSFILLLADTIGRVLLVGTGIPTGIIVSLIGAPYFLWLLKQEG"
     gene            complement(8983..10008)
                     /locus_tag="U1099_000458"
     CDS             complement(8983..10008)
                     /locus_tag="U1099_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM4866805.1"
                     /translation="MNKLVSSRYPKTKPKNIWLVFFIICLSFIAGVYLSLRFGAISYS
                     HQQLIETLKHPLTDSSAQDVIIDLRLPRMVAAILVGAAMAQAGAMMQGITRNPIADPG
                     LLGINAGAGLALIVAYALFGGLHYSQILLICLLGSCLAAGLVFGLAYQVQKGYNQLRL
                     ILSGAMVASLFSAIGQAITIYFDLSTAVIGWQAGGLVQVNWKMLAIIAPLIIIGLILA
                     QLFSHQLTILSLNETVAKNLGQRTLLMTLVLLGIVLLLSASAVALVGSLSFIGLIIPH
                     FIRMFTGKNYKLLLPLTAFAGASFLIWVDLVCRSINPPVETPISAVISIIGLPCFLWL
                     IRKEKHF"
     gene            complement(10022..10951)
                     /locus_tag="U1099_000459"
     CDS             complement(10022..10951)
                     /locus_tag="U1099_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4866806.1"
                     /translation="MKKFLAIFSLFVGLIFLTACSTSSSSTDVELSSMPKIEGITYHG
                     DIPKNPKKVVNFAYSYTGYLLQLGIDVSSYSLDLEKNSPVFGDKLKDVPQLTTADTEA
                     IAAQKPDVILVFAGDDNLETLKEIAPVIEITYGKSDYLKMLTDIGQIFGKEKEAQAWL
                     DQWDKKVATAKEELKGLIDTSSTFTVMDFFDKNIFLYGNNFGRGGELIYRALGFAAPA
                     KVQEDIINKEGWFGVSQEALPEYVGDYVLVNVNDKTKEAAASLKESDIWKNLPAVKNG
                     HVIEIDYNLFYFSDPMSLDLQIDAFVSEIQKLQ"
     gene            complement(10948..11748)
                     /locus_tag="U1099_000460"
     CDS             complement(10948..11748)
                     /locus_tag="U1099_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866807.1"
                     /translation="MSSIQAENIQVSYDNRIIIDELSTSIPKGNITTIIGANGCGKST
                     LLKALTRIIPVQEGAIYLDGQAISQLPTKEVAKKLALLPQVLEATEGISVYELVSYGR
                     FPHQNGLGHLTDQDREKINWALEVTQTAPYARLPVDDLSGGQRQRVWIAMALAQDTDT
                     IFLDEPTTYLDLNHQLEVLELLKELNQSRQKTIVMVLHDVNLSARFSDYMIAMKEGDI
                     RYHGSVSNMMTTEILRDIFSIEPQLMQVAGQDYPILLTYDLKNNKEKK"
     gene            complement(11901..13346)
                     /locus_tag="U1099_000461"
     CDS             complement(11901..13346)
                     /locus_tag="U1099_000461"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019792455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEM4866808.1"
                     /translation="MITIERVLEILKADANFRHIKQNDQTDSTWTDVQFDALSYDSRT
                     VSPTTLFFAKGLAFKKEFLEKAIEAGLAFYVSEIDYEVDIPAIIVHDIKQAMSLIAME
                     FHGHPQKQLKLLAFTGTKGKTTAAYFAFNILKQSHKPAMLSTMNTTLDGKTFFKSTLT
                     TPESLDLFAMMAEAVKNGMSHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMDNSRAVIVNAGMDHFEVVKEQVSSKDHDFYGPTSENQISQSAG
                     FDFTATGKLAGHYDIQLIGGFNQENAIAAGLACLRLGASLEDIHTGIAQTNVPGRMEV
                     LTQQNGAKVFVDYAHNGDSVKKLIDVVLEHQTGKVFLILGAPGNKGESRRKDFGLLLN
                     DYPQIEVILTADDPNREDPAAIAEQIRAHMTRPSNFILDREEAIRTAMSQTSSTKDAV
                     IIAGKGADAYQIVNGEKAAYDGDLEVAKQYL"
     gene            13492..14238
                     /locus_tag="U1099_000462"
     CDS             13492..14238
                     /locus_tag="U1099_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEM4866809.1"
                     /translation="MFYSYLRTLLTFLLWAINGNIHYHDREKILPQEENYILIAPHKT
                     FWDPVFLGYAAAPKQFIFMAKKELFKDRGFGWWISKCGAFPIDRENPGMAAIKYPVNM
                     LKKSDRSLVMFPSGSRHSSELKGGVAVIAKSAKVKLMPATYVGPMTIKGLLAGERIDV
                     AFGNPIDVSDIKRMDDTGTAEVTSRIEAEFKRLDDHAASFQTKKKPNILTYIYRIPVL
                     LLVALILGLTYAFSYIASFFWQPSTQLDKK"
     gene            14306..14968
                     /locus_tag="U1099_000463"
     CDS             14306..14968
                     /locus_tag="U1099_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEM4866810.1"
                     /translation="MDTIKTYIELLKEYKWQIALPAVAGLLMATFLIFSQPAKSDQTG
                     LTDFPQTEQTSSGQEQTEEISTEVSEEPSQLVVDVKGAVDKPGLYTLEAGARVNDAVE
                     AAGGLTSQADPKSINLAQKLSDEAVVYVASKDENISVVTSTTASSAMSPEEKSTSLVN
                     LNTATEADLQTISGIGAKRATDIIAYREANGGFKSVDDLNNVSGIGDKTMESIRPYVT
                     VE"
     gene            14952..17189
                     /locus_tag="U1099_000464"
     CDS             14952..17189
                     /locus_tag="U1099_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEM4866811.1"
                     /translation="MSRLSKLPCQPVHLAVLAVAAYFAVHSFSLLTMGLFSLLLAVFG
                     VRQGKTVFLKTLPLLALCGLFFGYQKVQWERADQSATEQVTTVQVIPDTIDINGDSLS
                     FRGRADGQTYQVFYKLTSQEEQTYFQKLTGLVQLEVEAEISLPAGQRNFKGFDYQAYL
                     KTQGIYRTVKITAIKKIVPVQSWNVFDWLSNWRRQALVYVKTNFPAPMSHYMTGLLFG
                     DLDSDFDQMSDLYSSLGIIHLFALSGMQVGFVIDKFRWILLRLGLTKETVDKLQIPFS
                     LVYASLTGFSVSVVRSLVQKILGNLGLRKLDNFAVTVFVCLLILPRFLLTAGGVLTFT
                     YAFLLTVFDFEDLGQVKKAAVESLSISLGILPVLMTYFYAFQPLSILLTFAFSFVFDV
                     LLLPGLSVIFLLSPIVKITWVNGFFVFMEKIIVWVADLGLRPWILGKPSELVLLLLLV
                     SLFLLYDFHRRKKWLLGLSLVLALLFFITKHPLENEVTVVDIGQGDSIFLRDMRGRTV
                     LIDVGGRVDFAAKEAWQERPRQANAERTLIPYLHSRGVDRIDSLVLTHTDTDHVGDVL
                     EVAKQVQIGRIYVSPGSLTVPDFVATLKEINVPVHVVEVGERLPIFDSYLEVLYPDGT
                     GDGGNNDSIVLYGRLLKTNFLFTGDLEQGELDLIESYPNLPVDVLKAGHHGSKGSSYP
                     EFLDHIEAKIALVSAGENNRYKHPHQETLERFDSRNIQVYRTDQQGAIRFRGWREWRI
                     ETVRR"
     gene            17513..18169
                     /locus_tag="U1099_000465"
     CDS             17513..18169
                     /locus_tag="U1099_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM4866812.1"
                     /translation="MSVKDFMTRKVVYISPDTTIAHAADIMREQDLHRLPVIENDKLV
                     GLVTEGTIAEASPSKATSLSIYEMNYLLNKTKVKDVMIKNVITVSGYASLEDAAYLMY
                     KNKVGILPVVDNGQLYGVITDRDIFAAFLHVSGYGEEGVRARFLVENKAGELEKIIRL
                     VADKDYNIVSTVQLATKSGKVVIEVQIDGKVDVEEVRTLFEGAGIQVDSLVPTVAKNI
                     "
     gene            18263..19090
                     /locus_tag="U1099_000466"
     CDS             18263..19090
                     /locus_tag="U1099_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866813.1"
                     /translation="MGHKYKKYKKYGKNNSGLVALLVLLAIGFVMSFWYILLPTGLLI
                     GYFYKREAIQRFLNRDSIKRSQVLALSIRSGYEKHQELFVEKGEDKVVLQLRNRLLGQ
                     LFELDQLYQKVGKYLNGYESKEVVDTLQLKYHLKLPEAKTEKNTDTQVSTGGNAVMDE
                     QGMIADLAPEILETYCNIQRDNLVILEKLEKATDKREELTAIHEANMNRFNDILQGYL
                     KIKKSPKDYFNAEERLVQAKSAMEMFDKDLDDTIKQFNEAGMKDFEVSLRMMKREEE"
     gene            19096..20367
                     /locus_tag="U1099_000467"
     CDS             19096..20367
                     /locus_tag="U1099_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEM4866814.1"
                     /translation="MSGFNFDIDQIANNSLSTKDKTTELIQATPTGDNSKVSFLAQLT
                     PEQQTGIRAKAPQLVDNFLANQNALLDFGKEAVEEVNATVNHILSEQKKIEIPQVDEL
                     LANTNRELNGFVAKYKDISTTAELEKKPGFFQRLFKQTKNDLQEFYFDSQTIEKKMDS
                     MAASVVKQEEVLARNIVSAELLIENNTKSIENLVGVIAFIEATGQEAAERAKLAQENL
                     ATLVPTTVDYQVASEKLARVTEVANILEQQHSEYMSRLYVAWTTTPQMRNLVKVSSDM
                     KQRLGMLRRNTIPTMKLSIAQLGILQQSIKSSQTADAIVNANNAALQMLADTSKEAIP
                     MMERTAQNPTLSVASVTKLAESLVAQNNGIIAAIDEGRQKRRELEAAIVRSAETINDS
                     VKLRDQKIIAALLEQGKEAQSEAEQPLETNQ"
     gene            complement(20404..21270)
                     /locus_tag="U1099_000468"
     CDS             complement(20404..21270)
                     /locus_tag="U1099_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM4866815.1"
                     /translation="MVRLKNILLILLGAGLFAFGLNYLIMPNRLFEGGATGLTLIIYY
                     LFHIQPWIMNIVINIPLFILGWKILGKKTLYLSILGTFSVTFWLAIFEKIHFSINLQQ
                     DLILVSILGGILMGLGLGTIFRAGGTTGGSDIIARIGHKFLPYSIGQIILAVDILILT
                     LIVIVFKDLRTVLYTLMMVAIASKVIDFVTEGGYGSKGVMIVSQKSDQLAQAIDCEIE
                     RGVTFIKAQGFYSKADVNMIYSVIYKSQLQEMKELIHRIDPHAFITITDAHEVLGEGF
                     TLDRDKKPIERH"
     gene            21445..22083
                     /locus_tag="U1099_000469"
     CDS             21445..22083
                     /locus_tag="U1099_000469"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEM4866816.1"
                     /translation="MRNGFFITFEGPDGAGKTTVLQQLLPALQELGPEVVTTREPGGV
                     AIAEEIRNIILNPANTEMDDKTELLLFIAARRQHLKEKILPPLAQGKLLLIDRFIDSS
                     IAYQGFGRGLDIGDIHWLNQFATDGLKPDLTLYFDIDTEEGLARIARNADRDVDRLDM
                     EKADMHRRVRQGYLNILEQEPERFVKIDASQPLEAVVADALAVIKKRFAERT"
     gene            22080..22964
                     /locus_tag="U1099_000470"
     CDS             22080..22964
                     /locus_tag="U1099_000470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEM4866817.1"
                     /translation="MKIEELRNLQPNVFQRFVTILEQRRLVHAYLFSGDFASFDMAIF
                     LSQSLFCQEKEGVLPCQKCRSCRLIEANEFSDLTVIAPQGNVIKTDTIRELVKNFSQS
                     GFESNKQVFIIRDAEKMHANAANSLLKVIEEPQSAIHILLLTNQEEAVLPTIKSRTQI
                     IGFPKNIPAMERMLEEEGLLKTQANLLAQLVSSQEEAKKLAENKNFLDLMGQTKKFTD
                     LLLVEPTRAYLQVGILVSLAVEKAEQGRLFDLLSLILSEKMMESANVREKMDAVLKAK
                     KMWQANVSLQNSLEYITL"
     gene            22984..23301
                     /gene="yabA"
                     /locus_tag="U1099_000471"
     CDS             22984..23301
                     /gene="yabA"
                     /locus_tag="U1099_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEM4866818.1"
                     /translation="MDKKEIFDALDDFSQNLLTTLAEVDAIKKHLQGVIDENTTLRLE
                     NSKLRERLEKEDKTGHKSSNFGKENLENIYEDGFHICTFSYGQRRDNDEPCMFCVELL
                     NRD"
     gene            23303..>24035
                     /gene="rsmI"
                     /locus_tag="U1099_000472"
     CDS             23303..>24035
                     /gene="rsmI"
                     /locus_tag="U1099_000472"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEM4866819.1"
                     /translation="MKVQKSFKGQTSFGTLYLVPTPIGNLQDMTFRAVQILKEVDIIA
                     AEDTRNTGLLLKHFEVETKQISFHEHNAHEKIPVLIDWLKSGQSIAQVSDAGLPSISD
                     PGHDLVGAAIAEDIPVVALPGASAGITALIASGLAPQPHIFYGFLPRKSGQQKEFFQE
                     KKTYPETQIFYESPYRVADTLENMLTVYGDRQVTVVRELTKLYEEYQRGSISELLEYL
                     EENPLKGECLIIVEGAGEEVYPSADE"
BASE COUNT         7036 a         4563 c         5457 g         6979 t
ORIGIN      
        1 atattgttac tacttttgta tctgtatagt tatttagaca ttggacaggg aggatttatc
       61 tataatgagt tttaaagagg agagtataaa aatgattagc agtatgttag agcgggtaga
      121 gcattttgcg acagaaaatc ctgactaccc tgtttaccat tatctaggac agttgtatag
      181 ttatggggat ttaaaggcag attcagattc tttagcagcc catattgata gtttggattt
      241 accagcaaac tctcctgtcc ttgtttttgg cggacaggag taccagatgt tggcaatctt
      301 tgttgcctta accaaggctg gccatccgta tatccctgta gatagtcatt ctgctttgga
      361 tcggattgaa gctattctgg aaatagcaga gccgagtttg gtgtttgcgg tagcagactt
      421 tccgctagag acaagtttgc cagtgcttgg tttgtctgaa gttgaagcca tttttgagaa
      481 gaagtcgact tatcagttga tgcatcctgt aagtggagat gatacctact atattatctt
      541 cacttctggg acaacgggta aaccaaaagg ggtgcaaatt tcgcatgaca atttgctcag
      601 cttcactaac tggatgataa gggataagga gtttgcgcta ccagagcgac cgcaaatgtt
      661 ggctcaacct tcctattcgt ttgacttgtc ggttatgtat tgggctccga cgcttgccct
      721 tggtgggacc ttatttgccc taccgaaaga gttgactttg gattttaaga ccttatttgc
      781 agctattcaa actttgccat ttaagatttg gacttcgaca ccatcatttg tagatatggc
      841 tttgctatct cgtgagtttg atggggaaca tttaccagat ttgacgcatt tctattttga
      901 cggagaggag ttaactgtaa aaacagctca gaagctccgc gaacgttttc caaaagctgt
      961 gattgttaac gcttatggac cgaccgaagc tacagtcgcc ttatcagcag ttgccatcac
     1021 tgatgagatg ctggtgaatt ataaacgttt gccgattggc tatgccaaac cagactcgcc
     1081 aacttttatt ttggatgagg atggtcaagt agtaccgaat ggtcaacagg gagaaattgt
     1141 tgtatcaggt cctgctgtct ctaaaggata tttgaataat cctgagagaa ccgaggcggc
     1201 atttttccag tttgacggct tacaggccta tcatactggg gatttgggtg taatgacaga
     1261 tgaaggtcta ttactctatg gtgggcgtat ggatttccaa attaaatata atggttatcg
     1321 tatcgagttg gaagaagttg cccaacactt aaataaatca acctatattg aggctgcggt
     1381 agctgttcca cgttataatg ctgaccataa agtgcaaaat ctgttagctt atgttgtccc
     1441 taaagcaggg attatggagc agttcgatcg aaagttggag ttaaccaagg tcatcaagca
     1501 ggagttaaaa gaacggatga tggattatat gatgccttcg aagtttattt atcgagacag
     1561 tcttcctttg actgcaaatg ggaagattga tattaaggga ttgatcgctg aggtgaatgc
     1621 tcaatgatgg agtttttcaa acagctacct cagttagagc cttatggaaa tccattgtac
     1681 tttttctatc tagtcttggc gctagtacca atttttatag gactcttttt taaaaaacgt
     1741 ttccccttgt atgaaacagg tgttagttta gccttcattg tcttgatgtt tacaggtaca
     1801 aagacaatgc aattgctgtc tttattggcc tatattattt ggcagacaat gttgattttt
     1861 ttctacaagc attatcgtca acgggcaaat caaagctggg tcttctattt aatcgtgtgt
     1921 ttagctgttt ttccgttgac ttgggtaaag ttggcaccga cttttagtca gcatggggca
     1981 attttcggtt ttttaggtat ttcttattta acgtttcggt cggtcggaat ggtgattgaa
     2041 atgcgggacg gtttactaac ggaatttagt ttgggaagtt ttttgcgatt tttgctattt
     2101 atgccaacta tttcgagtgg tcctattgat cgttatagac ggtttacgga agattacaaa
     2161 caaattcctg agcgaactga gttattaaat atgctcgatc agactatgca ttatattatg
     2221 atgggcttct tatataagtt tattctagcc tattttatcg ggcatacttt actggagcct
     2281 ttaaaggcag tagccttgga tcaaggtgga tggtttaact tacctactat tggagttatg
     2341 tatctttatg gttttgaatt attctttgac tttgcaggct attccatgtt tgccataggt
     2401 atttccaatc ttatgggtat tcgctgccca attaactttg atcaaccgtt caaatcgcgt
     2461 gatcttaagg aattttggaa tcgatggcat atcagcttat ctttttggtt ccgtgatttc
     2521 gtcttcatgc gtctggttaa gactttgttg aaacataagg ttttcaaaaa tcgaaatacg
     2581 gtatctaatg ttgcctatct gttaaatatg ctcttgatgg gcttgtggca tggtgtaaca
     2641 tggtattata ttgcatatgg tctctttcat gctttggggt tgatcatcaa tgatgcttgg
     2701 attcgtaaga agaaatcaat taatttagct cgaaaaaaag ctgggcaaga accattgccg
     2761 gataatcgtt ggacgagttt cgctggtatg tttgtgacct tccatacggt catgttctct
     2821 ttcttgatat tttcaggatt tttagataaa ttgtggttca agtagacaat aggtaacgat
     2881 tgtataaatc gtagaaagga atataaaatg gatgtaaaag aaacaatatt gaatattatc
     2941 gaagaattat ttatggaaga tgtatctgaa atgatggatg aagacctatt tgatgcgggt
     3001 gttttggata gtatgggaac tgtagaactt attgtggagt tagaaagtcg ttttaatatt
     3061 acagttcctg tttcagagtt tggtcgtgaa gattggaata cagctaacaa aatcatttct
     3121 ggtgttgtgg agttgatgca tgcgtaagca attgtggcag atttttgggc ctgtgatttg
     3181 tgcaacgttt ttagttattc tcacgttgtt gtttttcccg agttcttctc ggtcaaattt
     3241 caaagaagaa aaacaagatg cagtagcatt gtcttatatt agttttaaga gtaaatataa
     3301 gaaaaatagg gctctgggag acaaaaatca tcgttttgtt cctttctttg ggtcaagtga
     3361 atggtcacgt tttgatagta tgcatccgtc agtgttggcg gatgcatatc agagagagta
     3421 taccccctat ttgttaggac aacgtggtgc agctttgctg acacaatatt ttggcatgca
     3481 acagattggg gcacatttaa aaaatagtca ggctatctat gttgtttctc ctcaatggtt
     3541 ttcacccaaa ggggcatcat ctaaggcatt tgaacaatat tttagtagtg atcaagctac
     3601 tcattttctg caaacaagtg ctggggatga ctatgataga tatgcagcaa agcgtttctt
     3661 agagcttaaa tcagactgta gtttgacagg aatgtttcaa aaagtagcta gtggtaagga
     3721 attgtcgaaa tgggatagag agcagttgac ctggcaagcc tatttattag aaaagcaaga
     3781 tgccttattt agtcgctttg ctgattctgg tagtaattta ggattggtag aaacggaagc
     3841 gatgaaactt ccagagactt tttcctatga ccgcttgagt aaagtggcta atgaggacgg
     3901 gagtcgttct actcaatcaa atgatttcca aattgatgat aggttttata gaaatcgtct
     3961 tcagtcaaaa atcaaaaaac tcaaacaatc ccagacaaaa atatcttatc tgcaatcacc
     4021 ggagtacaat gattttcagc tggttttgaa tgaatttgct aaacatcaga ctgatgtgct
     4081 atttgtaatt cccccggtta atgaacgttg gatgaactac actggtctca ataaagatat
     4141 gtatcgggca acagtagcaa agattaagta ccagttgacc agtcaaggtt tcaatcatat
     4201 tgctgatttg tcggagcagg gggggataga ttattttatg caagatacaa tccacatcgg
     4261 ttggcaaggc tggctggagc ttgataagta tatccaccca ttcttgacag aacaacaagc
     4321 tcctgttcat tatcagctaa aaaaagaatt tctaacttcc gaatgggcta catataaggg
     4381 agatttaaac ctatttttga aatagttaga tacagttata ataaaaagca agaaggcttt
     4441 gaattaggaa gtctccttgc ttttttgttc ttttactgta ataaactgaa tgattatata
     4501 tcttctccca acaaactgtg gttggctttg tggaagcgaa taacatgata ggcaatcata
     4561 gcgtgtgaga gcaggtttaa gataacaaat agtaggatat ttgaatgacc atccagcaat
     4621 aaggcaacat ttccaattag taaaatgaga gcatagatga acagccctct cctgccaact
     4681 tgatggactt gttgattata gaaggaggtc gaaaggatac cactatagaa gagaaggttt
     4741 cctacaatca gcatgggcaa ggccaaatca ttatgatgac tagggaaaag ctccattgcc
     4801 actgtgaaga aattcaagcc aagaacaatt accaagtgag cgtatagcag aacagtcgca
     4861 tctgccttgc ggtgatggtc gatagtgaga taggtctgag aaatatagaa gatgaacaga
     4921 gatgccattg ccaagaagag gaggattcct tccaaaggaa gttctagaat cggataattt
     4981 ttcaaaatca taatgaccgt ctcaccaaag gtgataatag taatcagctg gcagcgttct
     5041 acgatatgag gaaagttcat cacatcatgg ctgagtttgt ctttgaaaaa ataggaaatg
     5101 aaaaatggaa gataaaaaag caagaggctc ttttcatcat aggtccagaa attgaagtaa
     5161 atagctaccg aagccaagaa aaatactagc acataaatcc caaacatata gagactatgc
     5221 ttaatatcag ccgtaaaacc gatttttcga ccccgaaggc agataagaca gggtcaaaaa
     5281 gatagtaaag ggtagggctg tcgctttaaa atcatgacta aagtttaggg ataattgccc
     5341 caccacaaac atagaagcga tgatagtgaa aatatccagc aaatctcgct caccatattt
     5401 gttgaggtaa atcgtctcat taatccacaa ggtcataata atcaggttag cggtgataaa
     5461 agttagaatt tccctgagtc cgatatggtc cccgtgtaag aggccagtca gtgaagaggt
     5521 agctaataca aagacaaatc ataaaataat tcatagttgg aaacacgttt tgctgtaatt
     5581 tcagacataa aaatctcctt ttcaaaaaaa tctgacctgt tataccatac gaaaagggag
     5641 gctgtcatct ccctttattt cttacaatgt ccagtccaag tctggtccga ctgggacaat
     5701 acctgttggg ttaatggttt tgtggctacc gtagtagtgc tgtttgatat ggtcaaagtc
     5761 aaccgtctcc gcaataccag agtggttgta aatccgcttg gtataatccc acaagtttgg
     5821 ataatcaacc aaggccttga tattgcattt gaaatgccca aagtaaacag gatcgaatcg
     5881 taccaaagtg gtaaagaggc gaatatcagc ctcggtcaat tgattgccca ccagatagtc
     5941 cttgtctgcc aaatgctctt ccaatttatc taaggcggca aaaaggttct tgacctcttt
     6001 ttcatagact gcctgactgg ttgaaaaacc tgccttgtag actccgttgt tgatgtttgg
     6061 atagataaaa tcattcatgg catcaatctc ggcttgcagg tttgctggat aatagtcatc
     6121 gtaatttcca gtaatatcat taaaagcaga gttaaacatg cgcatgattt cagccgattc
     6181 attgctgacg atagtatttg tttctttgtc ccaaaggaca ggcacggtca cccgaccaga
     6241 atagtgtgaa tcagccttca aatagacctg atagagataa tcgctgttaa agagggtatc
     6301 cttgataaca ccaggaccat cttcaaatgt ccagccattt tctaacatga ggggatgaac
     6361 gacagacaag gaaatatggt cttccaagcc cttcaatttt cgcataatca aggtccgact
     6421 agcccaagga caagccagag aaatatagag gtggtagcga ccagactcag ccttgaaacc
     6481 gccctctcct gttggacctg cctgtccgtc tggcgtaatc cagttgcgaa attgtgtgac
     6541 gctacgaata aacttaccgc ctgttgactt ggtgtcatac cattgatcca cccatttgcc
     6601 atcttgtaaa agtcccatga attttctccc ttactgaaaa aatgggaaat ccaataggat
     6661 gattggattt cctttgtaaa aaaatatatc actaactagt actataaact tttttcaaaa
     6721 agagtcaagc tatatgattt gtgatacaat agtcccatga gattagacaa atgtttagag
     6781 aaagctaaag ttggctcgcg taagcaagtt aaaaaacttt tcaaagccca gcaaatcaag
     6841 attaacggac aagcagctca aagcctaagc cagattgttg acccagagtt acaaactatc
     6901 caagtttcgg gtaaaaaagt tgccttagaa ggttccgcct attatttgct ccataaacca
     6961 gcaggagtcg tttctgcagt gacagaccag gagcatcaga cagtcatcga cttgatttct
     7021 ccgcaagaca gtagggaagg tctctaccca gtcggtcgcc tggacaggga caccgaaggc
     7081 ctggtcctca ttaccaacaa tggtccgctc ggttatcgca tgctccatcc cagccaccat
     7141 gtggacaagt tctactatgt ggaggtaaat ggatgcttgg ctgaggatgc ttcaaaattt
     7201 tttgcctcag gtgttacttt cttggatgga acacggtgtc agcctgcagt tttgactgtc
     7261 ttggaggctt ctttggacca tagcagagcg accatcaagc tggcagaagg taagtttcat
     7321 caggtcaaaa agatgttttt agcctatggt gtcaaggtca cttatctcaa gcggatttcc
     7381 tttggcggtt ttgagttggg ggatttggaa cgaggtgcat acagacaact cacaccaaat
     7441 gaaatggaac atttattcac ttattttgac tagaggagga tatataatga gcatttatga
     7501 ttttacagta caaaaacaag atggaacaga tcagtccata gctgagtatc aaggacaagt
     7561 tttacttata gtcaatacag cgccaggttg tggattggca ccacaataca aggaactaca
     7621 ggagctatat gatagctatc gaggccaagg ttttgtagtg ttggatttcc cttgtaatca
     7681 atttttaaat caggctcccg gctctgcgga agacatcaat cagacctgta gcttgaatta
     7741 tggtacaact tttcctcgtt ttgctaaaat tgctgtgaat ggatctgaag ctagtccgct
     7801 gtatcgctat cttaagaaag aaaaatcaac cttattagga ggtcgaattg agtggaattt
     7861 taccaagttt ttggtggata gacaagggcg tgtggtcaaa cgatatttac caacaactag
     7921 tccattaaaa cttaaagagg atattgaatt ttacctagaa aaataacaag aaggctggag
     7981 tcatctagcc ttcttgtttt agtaaccaca gaaaataggg agcgccgata agggagacga
     8041 tgatgccggt tgggatgcct gttccgacaa gtagtacccg tcctatggta tccgctagaa
     8101 gtagaatgaa gcttcctatt atcatagtag ctggaatgat gacacgatgg tcatttccta
     8161 accatttgcg cacaatgtga ccagcaatga gaccaacgaa ggtgatatta ccaactaaaa
     8221 tgacactaag agaggctagg gcggtagcca atatcaaggt aatccgcctt tcacgctgta
     8281 agtggagtcc caaagccatg gctgtctggt cgtttagatt cataatattg agtgagcgac
     8341 ttcgactaaa cgttgccatc cataccaata gaagaagagg agtatagatt gcgatagttt
     8401 tccaatctcc accatttact cgaccgctga gccactggac aatgtattcc atcttaaagt
     8461 catctaattg gctgatgatg ctgaccatga taccagacag catgctcgaa attccaactc
     8521 ccgtgataat taagcgtgca ggatggatgc cgtacagctt cttacgtgca atccagtaaa
     8581 ctagaaaaat ggttaatcca ccgcccatca tagcaaatag aggcatgaga ctcaataaga
     8641 cagggctgat ttttgaagag agaccaacaa atagtgccac aacaagacca gctcctgcgt
     8701 tgattcctaa tattccagaa tctgccagag gattgcgggt caaagtttgt agcaacaaac
     8761 ccgccaaagc cagagaagcc cctccgataa tggaggcgat tattctaggt aagcgaattc
     8821 gcccaataat cagtagagtc gatgaactgg cacgcccact catgatgtcg atgagatctg
     8881 aaaaggatga attgctatag ccgatagata aggaaagtag gaaaatgaca aataaaagga
     8941 tcagcaggaa tgtgaatatt tttagatagt tgtgatgtct tatcaaaagt gtttctcctt
     9001 tctaatcaac cataggaagc aagggagacc aatgatactg atgactgcgc taatgggagt
     9061 ttcaacaggt ggattgatag aacggcaaac caaatctacc cagatgagga aactagcccc
     9121 tgcaaaggca gttaatggca agagtagctt gtaatttttc cctgtaaaca tgcggataaa
     9181 gtgaggaata atgagcccga taaaagaaag cgagcccaca agagctaccg cagaagctga
     9241 gagtagaagg acaattccca acaaaaccaa ggtcatcagt aaggttcgtt gacctagatt
     9301 tttagcaacg gtttcgttga gactcaggat ggtcagctga tgcgaaaaga gttgagcaag
     9361 gatgaggccg atgatgataa gaggagcaat gatggccagc atcttccagt ttacctgtac
     9421 caatccccct gcttgccagc caattactgc agttgataaa tcaaagtaga tagtaatagc
     9481 ctgaccaata gcagaaaata gacttgctac catggcgcca gataaaatta aacggagttg
     9541 attgtagcct ttttgcacct gataagctag accaaagact agaccggctg ccagacagga
     9601 gcccagtaaa catattaata aaatttgact gtagtgcaaa ccaccgaaca gagcataggc
     9661 aacaatgaga gcaagtcctg ctccagcatt gattcctaat aagccaggat cggcaatcgg
     9721 attgcgtgta attccttgca tcattgcccc agcttgggcc attgcagctc ccaccaagat
     9781 ggcggctacc attctaggca agcgcaaatc gatgataaca tcctgagcgg aagaatctgt
     9841 cagtggatgc tttagggttt ctatcagttg ttggtggcta taagaaatag ctccaaagcg
     9901 taaactaaga tagactccag ctatgaaaga aagacagatg atgaaaaaaa caagccaaat
     9961 attctttggc tttgttttag gataacgaga acttactagt ttgttcatat atccagtcct
    10021 tttactgaag tttttgaatt tctgagacaa aggcgtcaat ttgtaaatct aaagacatcg
    10081 gatcggagaa gtagaagagg ttatagtcta tctcgatgac gtgaccattc tttacagcag
    10141 gtagattttt ccaaatatca ctttctttga gagaggcagc tgcttctttt gttttatcat
    10201 tgacgttaac aaggacatag tcgccaacat attctggaag agcttcttga gataccccaa
    10261 accagccttc tttattgatg atgtcttctt gaacctttgc aggagcggca aaaccgagag
    10321 cgcgatagat taattctcca ccacgaccaa agttattacc ataaaggaag atgttcttat
    10381 cgaaaaagtc catgacggta aatgttgaac ttgtatcaat caatcctttc aattcctctt
    10441 tcgcagtagc aactttctta tcccattgat ctaaccaggc ttgtgcttcc ttttcttttc
    10501 caaaaatctg accaatatct gtcagcattt ttaagtagtc actctttcca taagtgattt
    10561 caataactgg agcaatttct ttcagtgttt ctaagttatc atcaccagca aatactagaa
    10621 tgacatctgg cttttgtgcg gcaatcgctt ctgtatctgc cgttgtcaat tgtggaacat
    10681 cctttagttt gtctccaaag acgggactat ttttctctaa atctagacta tagctagata
    10741 catcaattcc taattgtaag agatagcctg tgtaagagta agcaaagttg acaacttttt
    10801 taggattttt tggaatgtca ccgtggtaag taataccttc aatctttggc atgctagaca
    10861 attctacgtc tgttgaagag gaagaggtag aacaagctgt taagaaaatc agcccaacaa
    10921 ataaactaaa aatagctaaa aactttttca ttttttctcc ttattatttt ttaaatcgta
    10981 agttagcaaa atagggtaat cttgaccagc aacctgcatc aattgaggtt caatgctaaa
    11041 aatgtccctc agtatctcag ttgtcatcat attggacaca gacccatggt agcgaatatc
    11101 tccctctttc atggcaatca tataatctga aaatcgagct gataaattga catcgtgaag
    11161 gaccatcaca atggttttct gtcggctttg attcagttct tttaaaagtt ctaagacttc
    11221 caattgatga ttgagatcaa gataggttgt tggttcatct aagaaaatgg tatccgtatc
    11281 ttgggcaagt gccatcgcaa tccaaacacg ttgcctctga ccacctgata aatcatcaac
    11341 tggtaaccta gcgtagggag cggtctgggt gacttccaag gcccagttga ttttctctct
    11401 atcctgatca gtgagatgcc ccagcccatt ttgatgcgga aagcgaccat aggagaccag
    11461 ttcataaacg gaaatccctt ctgtcgcttc taagacctga gggagcaggg ccaatttttt
    11521 tgcaacttct ttggttggca gttgtgaaat ggcttgtcca tctagataga tagccccttc
    11581 ttgaacagga ataatccttg tcaacgcctt aagcaaggtc gattttccac agccattggc
    11641 accaatgatc gtagttatat ttccttttgg aatacttgtt gataattcat caataataat
    11701 ccgattgtcg taagagactt ggatattttc cgcttgaatc gaagacacaa actcctcctt
    11761 tccagaatat gattcattaa taatttgttc cgaccagtat tatatcaaaa aaatttacca
    11821 aaacaaagag ttttctgaaa attttaattt aatgagaatc gttataaaaa aagtccttgt
    11881 ttgtgaacaa ggaccgtatg ttacaaatat tgttttgcta cttccaaatc accgtcatag
    11941 gcggcctttt cgccgtttac aatctggtag gcgtcagccc ctttgcctgc gatgatgacg
    12001 gcatctttcg tagagcttgt ctgactcatg gctgtgcgaa tggcttcttc ccggtccaag
    12061 ataaagttgc ttggacgggt catgtgggca cggatttgtt cagcaatagc agctgggtct
    12121 tcacggttgg ggtcatcagc tgtcagaatc acctcaatct gcggatagtc attgagcaga
    12181 agaccgaagt ctttgcggcg actttctccc ttgttaccag gagctcccaa aatcaaaaat
    12241 accttgcctg tctggtgctc taagacgaca tcaatcagct tcttgacact atcgccgtta
    12301 tgggcgtagt ccacgaatac cttggcaccg ttttgctggg ttaggacttc catgcgacca
    12361 gggacattgg tttgggcaat ccctgtgtga atatcttcca gacttgctcc caagcggaga
    12421 caggctaggc cggctgcgat ggcattttct tggttgaagc caccaatcag ctggatgtcg
    12481 taatggccag ctaatttgcc agtcgctgtg aagtcaaaac ctgctgattg gctaatctgg
    12541 ttttcagaag tcggcccata aaagtcatgg tctttggagc tgacttgctc tttcacgact
    12601 tcaaaatggt ccattcctgc attgacaata accgctctgc tattgtccat caaaagacgc
    12661 ttatggtaga agtagtcctc aaaggtcgga tgctcaatgg ggccgatgtg gtcggggctg
    12721 atgttgagga agacaccgac gtcaaaggtc agcccgtaaa cccgcttgac caagtaagcc
    12781 tggctggaca cctccataat caagtggctc atgccgtttt taaccgcctc cgccatcatg
    12841 gcaaagaggt ccaggctttc gggcgtggtc agggtggact tgaagaaggt cttaccgtcc
    12901 agtgtggtgt tcatggtcga aagcatggca ggcttgtggc tctgtttgag aatgttaaaa
    12961 gcaaaataag ccgccgttgt tttacccttg gtgccggtga aggccaggag tttgagctgt
    13021 ttctggggat gaccatgaaa ttccatggca atcaggctca tagcctgctt gatgtcatgg
    13081 acgatgatag caggaatgtc aacctcatag tcgatttcag acacgtaaaa agccagtccg
    13141 gcctcgatag ctttttccag aaattccttt ttaaaggcca aacccttggc aaaaaagagg
    13201 gtcgtcgggc taaccgtgcg gctgtcatag ctgagagcgt caaactggac atccgtccat
    13261 gtgctgtcag tctgatcatt ctgtttgata tggcggaaat tagcatccgc cttcaatatt
    13321 tctaatacac gttcaattgt aatcatgctt ctattgtaaa cctatcgagg atatttgaca
    13381 agtctgggcc atgtaaatag tgggtatttt gaccgtaaat ttgaatgaat atgacatttc
    13441 gtactgacgc aaatttgtgc tataatagga gggattgaaa aggagaagtt tatgttttat
    13501 tcttatctac gaacactatt aacattcttg ttgtgggcca tcaatggcaa tattcactat
    13561 catgatagag agaagatttt gccacaggag gaaaattata ttcttatcgc tccgcacaaa
    13621 accttctggg atccagtctt tcttggctac gcagctgctc ctaagcagtt catttttatg
    13681 gctaaaaaag aactcttcaa ggaccgcggt tttggttggt ggatttcaaa atgtggagcc
    13741 tttccaattg accgtgaaaa tcctggcatg gcagctatca agtatccagt taacatgttg
    13801 aaaaaaagtg accgttcgct ggtcatgttt ccttctggaa gtcgtcattc atctgagcta
    13861 aaaggtggtg tggcggtcat tgccaagtca gccaaggtca aactcatgcc agcaacctat
    13921 gtgggtccca tgactatcaa agggctctta gctggtgagc gaatcgatgt ggcttttgga
    13981 aatcctatcg atgtttcgga catcaagcgt atggatgata caggtacagc agaagtaact
    14041 agccgaattg aggctgagtt caaacgctta gatgaccatg cggcatcttt ccaaaccaag
    14101 aaaaaaccaa atatcttgac ttatatctat cgtatcccag ttcttttgct ggttgctctt
    14161 atcttgggat tgacctatgc ctttagctat attgccagct tcttctggca accaagtact
    14221 cagttggata aaaaataagt aaatgaaact cgcagcttgc gggttttttt gtatttttta
    14281 cgaactatat aggtggaggt aatttatgga tacgattaaa acttatatag aattgcttaa
    14341 agaatacaag tggcaaattg ctctgccagc agtggctggc ttgctaatgg cgacgttctt
    14401 aatattcagc caaccagcca agtctgatca gacgggtctg actgattttc cgcagaccga
    14461 acaaacttca agcggtcagg agcagacgga ggaaatcagt acagaagtaa gtgaggagcc
    14521 cagccagctg gttgttgatg tcaaaggagc ggtggataaa ccagggcttt acaccttaga
    14581 agctggtgcg cgtgttaatg atgcagttga agcagctggc ggcttgacca gtcaggcaga
    14641 cccaaagtct atcaatctgg ctcaaaagct cagcgacgag gcggtggtct atgtggccag
    14701 taaggacgaa aacatctcgg tggtgactag cacaactgcc agctctgcta tgtccccaga
    14761 agaaaaaagc accagtctgg tcaatctgaa tacggcgacc gaggctgact tgcagaccat
    14821 ttctggtatt ggtgccaagc gagcgacgga cattatcgcc tatcgtgagg ccaatggtgg
    14881 cttcaagtcg gtggacgacc tcaacaatgt gtcgggcatc ggcgacaaga ctatggaaag
    14941 catccggcct tatgtcacgg ttgagtaagc tcccctgcca gcccgttcac ctggcagtct
    15001 tggcggtggc ggcctacttt gcagtccatt ctttttccct cttgacaatg ggcctgttca
    15061 gtctgttact ggctgtcttt ggtgttcggc aaggtaagac ggttttcctc aaaacgctgc
    15121 cgcttttagc tctatgtggt ctctttttcg gctaccaaaa ggtccaatgg gagcgggcag
    15181 accagtcagc cacagagcaa gtcacaactg tgcaggtcat tccagatact attgacatta
    15241 acggggacag cctgtctttt cgtggtcggg ctgacggaca aacctatcag gttttctata
    15301 aattaaccag tcaggaggaa cagacctatt ttcaaaagct gacaggtttg gtccagcttg
    15361 aagtggaagc agagatcagt ctgccagctg gtcagcggaa tttcaagggt tttgactatc
    15421 aggcctacct gaaaacacag ggtatttatc ggacggtcaa gattacggca atcaagaaga
    15481 ttgtcccagt ccagtcttgg aatgtctttg actggttgtc aaactggcgg aggcaggctt
    15541 tggtttatgt caaaaccaat tttccggctc ccatgagcca ctacatgaca gggctcttgt
    15601 ttggggactt ggatagtgat tttgaccaga tgagtgacct ctattccagt ttgggtatca
    15661 ttcatctctt tgccctgtct ggtatgcagg tgggctttgt catcgacaag tttcgctgga
    15721 ttttactgcg tttgggcttg accaaagaaa cggttgataa attacaaatt cccttttccc
    15781 ttgtctatgc gagcttgaca ggtttttccg tatcggtggt gcggtctttg gttcaaaaaa
    15841 ttttgggtaa tctgggcttg cggaagttgg ataactttgc ggtgacagtt tttgtctgtc
    15901 tgttgatact gccccgattt ctgctgacag ctggtggtgt cctgaccttt acctatgctt
    15961 ttttgctgac ggtttttgat tttgaggact tggggcaggt caaaaaggct gcggtggaaa
    16021 gtctcagtat ttcacttggg attttgccag tgctcatgac ctatttctat gcctttcagc
    16081 ccctgtctat cctcttgacc tttgcctttt cttttgtctt tgatgtccta ctcttgccag
    16141 gcttgtcagt catttttctc ctgtcgccaa tcgtcaagat tacttgggtc aacggatttt
    16201 ttgtctttat ggaaaaaatc attgtctggg tggcggattt gggattgcga ccttggatac
    16261 tgggcaagcc gtctgagctg gtccttttgc tcttgctggt cagcctcttc ttactctacg
    16321 atttccacag gagaaagaaa tggcttctag gactgagctt ggtcctcgct ctgctatttt
    16381 tcataaccaa acacccgctg gaaaacgagg tgacggtggt ggacatcggg cagggggaca
    16441 gcatcttttt gcgggatatg cgggggcgga cggtcctgat tgatgtgggc ggacgagttg
    16501 attttgcggc caaggaagcg tggcaggagc ggcctcggca ggcaaatgca gagcggactt
    16561 tgattcccta tctgcatagt cggggtgtgg ataggattga tagtttagtc ctgacccaca
    16621 ccgacacaga ccatgtgggg gatgtgctgg aagtggcgaa gcaggtccag attggtcgga
    16681 tttacgtgtc gccgggaagc ctgacggtgc ctgactttgt agccaccttg aaagaaatta
    16741 atgtccctgt tcatgttgtt gaggtgggcg aacgcttacc aatttttgac tcttacctgg
    16801 aagtccttta tccagatggg acaggagacg gtggcaataa tgactccatt gtcctttatg
    16861 gtcgcttgct gaaaactaat tttctcttta caggggactt ggagcagggg gagttggact
    16921 tgatagaatc ttatcctaac ctgcctgtcg atgtcctcaa agccggtcac cacggctcca
    16981 aaggttcttc ctatcctgaa tttttggacc atatcgaagc caagattgcc ttggtatctg
    17041 ctggagaaaa caaccgctac aagcatcctc accaagaaac cttggaacga tttgatagtc
    17101 gcaatatcca agtctatcgt acagaccagc aaggagccat tcgcttccga ggttggaggg
    17161 agtggaggat tgaaacggtg aggaggtaaa atgataaatc ctatgcactt atggttctat
    17221 agtttcaaag tttatgtgct cgctgcctta tctggctata ttctgcacta tgttactacg
    17281 ccagcttctg ttaacgactt caaagaggtt gctgccttat tggaaagatg ctaacaatct
    17341 gtgattgtag cagattgggc tatctcagtc agcagtcttg ctcattccat tgtatttcaa
    17401 atagtctata ttttgaattt aagaattgat tttcatttct tttcattaaa tattgaaatc
    17461 gttttcggat ttgtgatata ataaagtatg atttaccaaa ggaggaagtc ctatgtctgt
    17521 taaagatttt atgacccgta aagttgttta tatttcacca gatacaacca ttgcacacgc
    17581 tgcagatatt atgcgtgaac aggatttgca ccgtctacct gttattgaaa atgataagtt
    17641 ggttggtttg gtcactgagg gaacaatcgc agaagctagt ccatcgaagg caactagcct
    17701 atctatttac gagatgaact accttttgaa taagactaag gtcaaggatg tgatgattaa
    17761 gaatgttatc acagtctcag gttatgctag tcttgaagat gctgcttatc tcatgtataa
    17821 aaacaaagtg gggattcttc cagttgttga caacggccaa ctatatgggg tcattactga
    17881 tcgtgatatt tttgcagctt ttcttcatgt ctcaggttat ggagaagaag gagtacgcgc
    17941 tcggttcctt gttgaaaata aggcagggga gttagaaaaa attattcgat tggtggcaga
    18001 taaggattat aatattgtat ctactgtgca acttgctacc aagtctggaa aagttgtcat
    18061 cgaagtgcag attgacggaa aagttgatgt tgaagaagtt cgaactcttt tcgaaggtgc
    18121 aggtatccaa gtggatagct tggttccgac tgttgctaaa aatatttaaa aaccgagatt
    18181 tctcggtttt tttctttagc tatcattcta gtattttagg ggaatgtgat ataataaagt
    18241 atatcacgta tgaggaggct ctatggggca caaatataaa aagtataaga aatatggtaa
    18301 aaataattca ggacttgtgg ccctccttgt tttattggca atcggttttg tcatgagttt
    18361 ctggtatatc ttattgccta ctgggctctt aattggctat ttttacaaga gagaggctat
    18421 ccagcgtttt ttgaatagag attctataaa acgctcgcag gtattagcgc tatctattcg
    18481 ttcgggctat gaaaagcatc aagagctctt tgtggaaaag ggtgaggata aggttgtgtt
    18541 acagttgaga aatagactgt taggacaatt atttgaatta gatcagctct atcaaaaagt
    18601 tggaaaatat ctgaatggat atgaaagtaa agaagttgta gataccttgc aactaaaata
    18661 tcatttaaaa ctgccagaag ctaaaacaga aaaaaatact gacacacagg tttctactgg
    18721 tggtaatgct gtgatggatg aacaaggcat gatagcggat ttagctcctg aaattctaga
    18781 aacctattgt aatattcaaa gagacaatct tgttattctt gaaaagctgg aaaaggcgac
    18841 agataagagg gaagaattga cagctatcca tgaggccaat atgaatcggt ttaacgatat
    18901 tttacaaggt tatttgaaaa tcaaaaaatc accgaaagat tattttaacg cagaagaacg
    18961 attggtgcag gcaaaatcag ccatggaaat gtttgataag gatttggatg ataccatcaa
    19021 acaatttaac gaagcgggta tgaaagattt tgaagtcagt ctgcgtatga tgaaaagaga
    19081 agaggaatag agaagatgtc aggatttaat ttcgatattg atcagattgc taataatagt
    19141 ttatcaacca aagataagac aacagagctt attcaagcga ctccgactgg agataattca
    19201 aaagtgtctt tcttggcaca gttgacaccg gaacaacaaa caggtattcg tgctaaggct
    19261 ccccaattgg tggataattt cttagctaat caaaatgccc tcttggattt tggtaaggaa
    19321 gcggtagaag aagtcaatgc cacggtcaat cacattttgt cagaacagaa gaagattgaa
    19381 attccacagg tggatgagct tttggccaat accaaccgtg aattgaatgg ttttgtggct
    19441 aagtataagg acatttccac aactgctgaa ttagaaaaga aaccaggttt cttccaacgc
    19501 ctgttcaagc agaccaaaaa tgatttgcag gaattttatt tcgactcgca gaccattgaa
    19561 aagaaaatgg atagcatggc agccagtgtg gtcaagcagg aagaggtgtt ggcgcgcaat
    19621 attgtttctg cagagctttt gattgaaaat aataccaagt ctatcgagaa tttggtgggg
    19681 gtcatcgcct ttattgaagc gactggtcag gaagcggcag agcgtgcaaa attggcacaa
    19741 gaaaacctcg caactttagt accgactaca gtcgattatc aagtagcttc tgagaagttg
    19801 gcacgtgtaa cagaggttgc taatatcctc gaacaacaac attctgagta tatgagtcgc
    19861 ttgtatgttg cgtggacaac gacaccgcag atgcgtaatc tggtcaaggt ttcttcggat
    19921 atgaaacagc gccttgggat gttacgtcgc aatactattc caacaatgaa attgtccatt
    19981 gcacagttgg gcatcttaca acaatctatc aagtctagcc agacggcaga tgcgattgtc
    20041 aatgctaata atgctgccct tcaaatgctt gcggatacat caaaagaagc catcccgatg
    20101 atggaacgca cagctcagaa tccgaccctt tcagtagcat ctgttaccaa attggcagaa
    20161 agtttggttg cccaaaataa tggcattatt gcagctatag atgaagggcg tcagaaacgt
    20221 cgtgagttgg aggcagctat tgtacggtca gcagagacta tcaacgattc tgttaagcta
    20281 cgcgatcaaa aaatcattgc tgctttgctg gagcaaggca aagaagcaca gtctgaggca
    20341 gagcaaccac ttgaaaccaa tcaataatat aaaacctgca gttcagcgaa actgcaggtt
    20401 tgtttaatgg cgttctattg gttttttatc tcgatctaaa gtaaatccct caccgagtac
    20461 ttcatgtgcg tctgtaatgg tgataaatgc gtgggggtca atacgatgaa taagctcctt
    20521 catctcttgg agttggctct tatatatgac cgagtaaatc atgttgacat cggctttgct
    20581 gtagaaacct tgtgctttga taaaggtgac accacgctcg atttcacaat caatagcctg
    20641 agccaattgg tcagattttt gagaaacaat cataaccccc ttgctaccat atccgccctc
    20701 agtgacaaaa tcaataacct tggaagcaat agcaaccatc attaaagtat atagaactgt
    20761 tcggaggtct ttaaaaacaa taacaatcag agttaggatg aggatgtcaa ctgcaagaat
    20821 gatttgtcca atggagtaag gtaggaattt atgacctata cgagcaatga tatcgcttcc
    20881 cccagtcgtt ccgccagcac ggaagatggt tcctaatcct agtcccataa gaatcccacc
    20941 caggatacta acgagtatca aatcttgttg tagattgatt gaaaaatgga ttttttcaaa
    21001 aatggccaac caaaaggtaa cgctaaaggt tcctaagata ctgagataga gagtcttctt
    21061 tccgagtatt ttccacccga ggatgaacaa tggaatatta atcacaatgt tcattatcca
    21121 aggttgaata tggaaaaggt agtaaataat cagcgtcaat ccagttgcgc caccctcgaa
    21181 cagacgattg ggcatgatta aataatttag cccaaaggca aatagccctg caccaagcaa
    21241 aataagtaaa atatttttca aacgaaccat aagcatctcc gaatatgtgt atatatcaat
    21301 tttaaaagat tttctcctta aaaacaagtc aattttcaag tgattttttg actaattttt
    21361 ggtaaaatgg aatgaatatc taagaaccag tcttcacaag tgaagtgctg aactgtgcat
    21421 actggtacta agagcgaggg aaagatgaga aacggttttt ttattacatt tgaaggtcca
    21481 gatggggcag ggaaaacgac ggtattgcaa cagttattgc cagccttgca ggaattgggg
    21541 ccagaagtag tcacaactag agagccaggt ggggtggcta ttgcagaaga aattcgcaat
    21601 attattctga accctgcaaa tactgaaatg gatgataaga cggagctgtt attgttcatt
    21661 gcagcacgac gtcagcattt gaaagagaag attcttccac ccttggcaca ggggaaattg
    21721 ctcttgattg atcgttttat cgattcctct attgcctatc aaggatttgg acgggggttg
    21781 gatataggtg acattcattg gctcaaccag tttgcaacag atggtttaaa gccagattta
    21841 accctctatt ttgatattga tacagaagag gggctggctc gaattgcccg caatgctgac
    21901 cgtgatgtgg atcgcttgga tatggaaaaa gcagatatgc atagacgggt gcgtcaaggc
    21961 tatcttaata ttttagagca ggaaccagag cggtttgtaa agattgatgc cagtcagcca
    22021 ctagaggctg ttgttgcaga tgctctggcg gtcattaaga aacggtttgc agaaagaaca
    22081 tgaagattga agaacttaga aacttacaac ccaatgtatt tcaacgtttt gtgacaattt
    22141 tagagcagag acgtttggtg catgcctatc tattttcagg tgactttgct agttttgata
    22201 tggcaatttt tctcagtcaa tcgttatttt gtcaggagaa agaaggcgtc cttccttgtc
    22261 agaagtgtcg gtcttgtcgt ctaattgaag caaatgagtt ttcagatctt actgttattg
    22321 caccgcaggg aaatgttatc aaaacagata cgatacgtga attagttaag aatttttcac
    22381 aatctggatt tgaatccaat aagcaggttt tcattattcg tgatgctgag aaaatgcatg
    22441 ctaacgcagc caactctttg ctaaaagtga ttgaagaacc tcagtcagct attcacatct
    22501 tattattgac caatcaggaa gaagctgtcc ttcctactat caagagtcgg acacagatta
    22561 taggttttcc taaaaatatt ccggccatgg agcgaatgtt agaagaggag ggcttgttaa
    22621 aaacacaagc gaatctctta gctcaactgg tttcaagtca agaagaagcg aaaaagttag
    22681 cagaaaataa aaacttttta gatttgatgg gacagacaaa gaaatttaca gacttgctct
    22741 tggtggagcc tacacgtgct tatttgcaag ttggaatcct agtgtcttta gctgtggaaa
    22801 aagctgagca gggacgtttg tttgaccttc tgagtctgat attatctgag aaaatgatgg
    22861 aatcggcaaa cgtacgtgaa aaaatggatg ctgttcttaa ggctaagaag atgtggcagg
    22921 caaacgtaag tctacaaaat agtctagagt atataactct gtaaaataaa agaaagggag
    22981 ataatggata aaaaagaaat ttttgatgcc ctagatgatt tttcgcagaa tcttctaacc
    23041 acgttggcag aagtggatgc tatcaaaaag catttacagg gagttattga tgaaaatacg
    23101 accctccgct tggaaaattc caagttgcgc gaacgtttgg aaaaagaaga taagacaggc
    23161 cataaatctt ccaactttgg taaggagaat ttggagaata tttacgaaga tggtttccat
    23221 atttgtacct tctcttatgg tcaacgtagg gataatgatg aaccttgtat gttctgtgta
    23281 gaattattga atcgagatta ggatgaaagt acaaaaatca tttaagggac agacgagttt
    23341 tgggacttta tatctggtgc caacgcctat tggaaactta caggatatga cattccgagc
    23401 tgttcagatt ttgaaagaag tggatataat tgcagcggaa gatacaagaa atacaggact
    23461 cctactcaaa cattttgaag tagaaaccaa gcaaatatcc tttcatgaac acaatgccca
    23521 tgaaaaaatt ccagttttga ttgattggct caaatctggt cagtccattg cgcaagtttc
    23581 tgatgcaggc ctgccttcca tttcggatcc tggtcatgac ctggtagggg cagcgattgc
    23641 ggaagatatt cctgttgtag cacttccagg agcatcggca ggaattacag ccttgattgc
    23701 atctggtttg gcaccccagc cacatatttt ttatggattt ttaccgagaa aatcggggca
    23761 gcaaaaagaa tttttccaag aaaaaaagac ctatcctgag acgcaaattt tctatgaatc
    23821 tccatatcgt gtggcagata ccttagaaaa tatgttgaca gtttatggtg atcgtcaggt
    23881 aacagttgtt cgagaattga ccaagttata cgaagaatac cagagaggaa gcatttccga
    23941 attactagag tatttggaag aaaatccact taagggagaa tgcttgatta ttgtcgaggg
    24001 tgctggtgag gaagtctatc cttctgctga cgaag
//
LOCUS       DASFSW010000014        23126 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.014, whole genome shotgun sequence.
ACCESSION   DASFSW010000014 DASFSW010000000
VERSION     DASFSW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23126
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.014"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1034)
                     /gene="serS"
                     /locus_tag="U1099_000473"
     CDS             complement(<1..1034)
                     /gene="serS"
                     /locus_tag="U1099_000473"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEM4866820.1"
                     /translation="MLDIKRIRTDFDGVAAKLATRGVAAETLANIKELDAKRREILVT
                     VEELKAERNTVSAEIAQAKRNKENADDKIAAMQKLSADVKNLDAQLLEIDEELNAIIT
                     VLPNTPHDSVPVGADEEENVEVRRWGTPREFTFEPKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIAEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRNEILEEKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFATPEQSYDELEKMTANAENILQKLGLPYRVLALCTGDMGFSAAKTY
                     DLEVWIPAQNA"
     gene            complement(1252..1623)
                     /locus_tag="U1099_000474"
     CDS             complement(1252..1623)
                     /locus_tag="U1099_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEM4866821.1"
                     /translation="MAQSQNTSVELQTTGVSYMGFGGKVGKFLIGDKALEFYPDSNVE
                     RYIQIPWSEMTSIGANVSGKAISRHFEIYTEKSRFLFASKDSGKILKIAREHIGNEKV
                     VKLPTLMQTIGRKISNLFAKK"
     gene            complement(1683..2594)
                     /locus_tag="U1099_000475"
     CDS             complement(1683..2594)
                     /locus_tag="U1099_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEM4866822.1"
                     /translation="MSEKIQLSVSDRKKVWWRSTFLQGSWNYERMQNLGWAYAMIPAI
                     KKLYTKKEDQAAALERHLEFFNTHPYVASPILGVTLALEEERANGAEIDDTAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLALSGNIMGPIIFFLGWNIIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSITFAVDLPAKQLSEGA
                     YIVFPEGTVTGAELKGILGQALSGLSLEPTQAQTLQGQLDSLIPGLMGLLLTFLCMHL
                     LKKKVTPITMIIGLFIVGILARFFGIM"
     gene            complement(2612..3421)
                     /locus_tag="U1099_000476"
     CDS             complement(2612..3421)
                     /locus_tag="U1099_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEM4866823.1"
                     /translation="MSDITLISAILVVIVAFFAGLEGILDEFQFHQPLVACTLIGLVT
                     GNLAAGVMLGGSLQMIALGWANIGAAVAPDAALASVAAAIIMIKGGDFSTEGIAVATT
                     TAIPLAVAGLFLTMIVRTISVGLVHGADNAAKDANFAAVERYHLFALLLQGLRIAVPA
                     ALLLAIPAEAVQAVLESMPAWLSGGMAVGGGMVVAVGYAMVINMMATREVWPFFALGF
                     AFAAISKLTLIALGVIGVAIALIYLNLSKQGGSGNGGASSNDPIGDILEDY"
     gene            complement(3453..4445)
                     /gene="manX"
                     /locus_tag="U1099_000477"
     CDS             complement(3453..4445)
                     /gene="manX"
                     /locus_tag="U1099_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEM4866824.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPSEGPDD
                     LYAKLNAAVDAFDADDEVLVLADLWSGSPFNQASRVAGENPDRKFAIITGLNLPMLIQ
                     AYTERMINAAAGVEEVAANIIKEAKDGIKVLPEELQPAEATPVADAAPVALQGAIPPG
                     TVIGDGKLKINLARIDTRLLHGQVATGWTPASKADRIIVVSDTVAKDELRKQLIKQAA
                     PGNVKANVVPIKKLIEVSKDPRFGNTHALILFETPQEALEAIEGGVEIKELNVGSMAH
                     STGKTMVNNVLSMDKDDVATFEKLRDLGVEFDVRKVPNDSKKDLFELINKANVQ"
     gene            complement(4667..5479)
                     /locus_tag="U1099_000478"
     CDS             complement(4667..5479)
                     /locus_tag="U1099_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM4866825.1"
                     /translation="MKKKIIAIDLDGTLLNNDSQLSDYTISTIKKVRQAGHTVLIATG
                     RPYRMAEKFYQQLELDTPMINFNGSLVHIPGKKWQWEQNILIDKKYLLEFLKEEETFE
                     ADFIAGEYKNKFYITQKNLDKIDPALMGVEQITPDTLIKPELITSDPHSILMQTRATD
                     KYELAKEMNAYFKDELEINTWGGPLNILETCAKGVNKATALSYVLNLYQATPSDLIAF
                     GDEHNDVEMLDLAGTAYAMKNCSDTLRPHADRLTAFANFEDGVARELENLFL"
     gene            5825..7284
                     /locus_tag="U1099_000479"
                     /pseudo
     CDS             5825..7284
                     /locus_tag="U1099_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
     gene            complement(7632..8492)
                     /gene="metF"
                     /locus_tag="U1099_000480"
     CDS             complement(7632..8492)
                     /gene="metF"
                     /locus_tag="U1099_000480"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEM4866826.1"
                     /translation="MIGQTPSLSFEIFPPKPEVGNEKIIQTLDEMQGLAPHFISVTCS
                     NNNLNVEETTVKLANHVRNELHIPTIAHLPAAYLTKEKVRSVLHSLDEIGVHQILALR
                     GDIIEGLPPKEDFQYATDLISFIKEEAPHFDIIGACYPEVHPESPNSVSDIKNLKKKV
                     DAGCSTLVTQLFFDNEAFYNFQEKCSLADIEVPIIAGIMPIINRNQALRLLKTCENIR
                     LPRKFKAILEKYEHNPESLRAAGLAYAVDQIVDLVTNDVAGIHLYTMNNAETARTIHQ
                     ATHSLFKHYS"
     gene            complement(8496..10745)
                     /gene="metE"
                     /locus_tag="U1099_000481"
     CDS             complement(8496..10745)
                     /gene="metE"
                     /locus_tag="U1099_000481"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEM4866827.1"
                     /translation="MTTTIIGFPRIGEHRELKFITEKYFRNEIPQEELLEVAKDLRAK
                     HWNIVKEAGITEIPSNDFSHYDNVLDAAVLFNIVPKAVQNLDLTDLEKYFALARGYQG
                     EKGDVRARPMKKWFHTNYHYIVPAIEKDTEIKLAGHKIFDEFQEAKNLGITTRPVLIG
                     PFTLLQLTDFEEGLTATDFADSLVAAYGQVFEKLAELGAEKIQLDEPSLVKDLTAEEK
                     ALFLRIYQTLLADKKGLQVLIQTYFGDVRDIYTELTSLPVDAIGLDFVEGKETAALVA
                     TGFPANKTLYAGIVNGKNIWRNNYEKSLAVLDAIPAEHVVITTSCSLLHVPFTTANEE
                     FEPAILNHFAFAVEKLSELRDLDAIRNSQGEVALTANKELFALERVGRDAALADRLAG
                     LTDADYTRLPVFAEREAIQREKLNLPLLPTTTIGSFPQTKEVRSTRLAFRKGNISEEE
                     YDTFVKAQTDEWIAWQEEVDFDVLVHGEFERNDMVEYFGENLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKE
                     STLQIALAIKEEVLDLEAAGIKIIQIDEAALREKLPLRRSDWYSEYLDWAIPAFRLVH
                     STVAPETQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLVILDELKAKNFQTQVG
                     PGVYDIHSPRVPAVDEIAHTIQAILAKVPKEKVWINPDCGLKTRGESETKASLIHLTQ
                     AAKTARKEL"
     gene            11125..11814
                     /locus_tag="U1099_000482"
     CDS             11125..11814
                     /locus_tag="U1099_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEM4866828.1"
                     /translation="MGKVIIGISGNEQEFPTKSGRTYVTVASELADGVRQAGGVPMVI
                     PMGTPDLAKDYINMIDKLILSGGQHVDPSLYGQERLIDSNDYLLERDAFELALIEEAL
                     RQGKPIFAVCRGMQLLNVALGGSLEQEVAHHWQEGIAGTSHRLQVKPKSRIGQLFTQG
                     SPINSFHQQRIKELAPGLVATGLDPRDGTIEAYESRGNQALFGIQWHSEFLYNDCKQH
                     RDLFQYLVDIF"
     gene            11966..12817
                     /locus_tag="U1099_000483"
     CDS             11966..12817
                     /locus_tag="U1099_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEM4866829.1"
                     /translation="MKLKKLFSLATAALSVGVLVACGNSSSSSDSSATTVRVGVMSLS
                     DSEQARWDKVQEILGDEVKLEFTQFTDYSQPNKAVAENEVDINAFQHYNFLNNWNQEN
                     GEDLVAIADTYIAPIRLYSGTADGKNKYTKVEEIPDGAEIAVPNDPTNESRALYLLQA
                     AGLIKVGVSGTELATIADITENKKNLKITELDASQTASSLSSVDAAVVNNTFVLEAGL
                     DYKNALYKEQKDENSKQWYNLIAARSDWEKSEQAAAIKKIIEAYHTDEVKKVIEETSD
                     GMDEPVW"
     gene            12974..14341
                     /locus_tag="U1099_000484"
     CDS             12974..14341
                     /locus_tag="U1099_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEM4866830.1"
                     /translation="MADSKVQAFENDAIIQNYFEKLKVLISKKSIFAQQIGLLDVATY
                     LKDMFEEAGADVVLDDSYAAPFVMATFKASVPDAKTLIFYNHYDTVPADADQVWEKGN
                     PFELTISDGYIYGRGVDDDKGHITARLSALKKYQARQNGDLPVNVIFIMEGAEESASV
                     DLDKYLSKYKEYLIGADLLVWEQGHRNSLHQLEIAGGNKGIVTFDLQVKSADLDIHSS
                     YGGVIDSASWYLLSALQSMRAADGEILVDGIYEQVQEPNERELALVEEFALATSQSMT
                     DIYGLTLPTLVEDRREFLKRLYFEPSITIEGLSTGYLGQGVKTIIPAQASAKMEVRLV
                     PGLEPHDVLDKIRQHLDKHGFDKVELVFTLGEMSYRSDMSHPAIVNVIELAKKLTPEG
                     VAVLPTSPGTGPMHTVFHALGVPIAGFGLGNANSRDHAGDENVSIADYYSHVELVEEL
                     IASYE"
     gene            14334..15398
                     /locus_tag="U1099_000485"
     CDS             14334..15398
                     /locus_tag="U1099_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEM4866831.1"
                     /translation="MSKEMIKLDNIDVTFQQKKRTIEAVKDVTIHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQVPTAGKITIDEDVIYEGEKVTLTPTQLRSKRREIGMIFQHFNL
                     MAQMTAEENVAFALKHSGLSKEEKKEKVAKLLDLVGLSDRAENYPAQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQELNEKLGLTIVMITHEMQIVKDICN
                     RVAVMQDGRLIEEGSVLEIFSHPKEELTQDFIKTATGIDEALVKIYQQDIVKNLPENS
                     ILVQLKYAGSNTDTAIVNDLYKFYQVSANILYGNIEILDHTPVGEMVVILSGEPGQLH
                     RAIEAVTEARVEVTILKGAN"
     gene            15399..16091
                     /locus_tag="U1099_000486"
     CDS             15399..16091
                     /locus_tag="U1099_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4866832.1"
                     /translation="MLEWIQTNFPDIYKLGWDGQTGWLTHFNLTLYMTFVSFAFGGFM
                     GLVSGLFLVLTGPRGVIANKTAYWILDKVASIFRAIPFIILLAAIAPFTKIIVGKTIG
                     TEAALVPLALSVFPFFARQVEVVLSELDRGVIEAAQASGATFWDIVLVYLREGLPDLI
                     RVTTFALVSLVGYTAMAGAIGAGGLGQVALSYGYLRYNNDVTFLATLLILVIIFVIQF
                     IGDFLTRKISHR"
     gene            16273..16977
                     /locus_tag="U1099_000487"
     CDS             16273..16977
                     /locus_tag="U1099_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEM4866833.1"
                     /translation="MSQFVVDSSFWSLFPDAKIGVLLLKDYKTPAQSPDELVKLLEES
                     NGIAQKFLTEDTFSENEVIRTYRQAYQKFKTKKGARSSIEALLKRSTSDRPVTTISPL
                     VDIYNAASLRFGLPCGAEDLDTFVGNLQLTVTEGGDEFYLIGDETNNPTLPGEVCYKD
                     DKGAVCRCFNWRDGERTMITDETKNAFLVMELVNSDRVEDLENALDFISQQAETFLGV
                     VPEKYLLDCDRPGIAL"
     gene            17244..17723
                     /locus_tag="U1099_000488"
     CDS             17244..17723
                     /locus_tag="U1099_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HEM4866834.1"
                     /translation="MWFFLSADRVFLVFHLKVKNSYYKERRPMFTLEAINHGHEQFTG
                     PDFPKLIAYFKDLGMVENIVDIQSGQVTYRSQTGQTLEKTGYQVTIPVSDQANLDQFV
                     TILRNHQAGQTDFPTFCQETAEAGVYKWVIDLEAMTCSYLDQAEQAVFVEAVPSVEN"
     gene            17929..18846
                     /gene="glyQ"
                     /locus_tag="U1099_000489"
     CDS             17929..18846
                     /gene="glyQ"
                     /locus_tag="U1099_000489"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEM4866835.1"
                     /translation="MSKKLTFQEIILTLQQFWNEQGCLLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLER
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEVSDQDMLLENFT
                     KFEKEAERALEEGLVHPAFDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAELLKEDAE"
     gene            18846..20882
                     /locus_tag="U1099_000490"
     CDS             18846..20882
                     /locus_tag="U1099_000490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEM4866836.1"
                     /translation="MTKNLLVELGLEEIPAYIVTPSMHQLRDRMATFLTDNRLAFDSI
                     DVFSTPRRLAVRVRGLADQQTDLTEDFKGPAKKIALDADGNFTKAAEGFVRGKGLTTA
                     DIEFREIKGEEYVYVTKHEAGQPAKAVLAGIPEVLKAMTFPVSMNWASNKFAYIRPVH
                     TLTVLLDDEALDMDFLDITSGRISRGHRFLGNETEIASADSYEADLRAQFVITDPAER
                     QNMIVEQIKAIEDKHNVTVEIDEDLLNEVLNLVEYPTAFMGSFDTKYLEVPEEVLVTS
                     MKNHQRYFVVRDKAGKLLPNFISVRNGNDQYLDNVIKGNEKVLVARLEDGEFFWREDQ
                     KLKIADLVERLKVVTFHEKIGSLYEHMERTKVIAEKLADLAGLSAGEKADVARAADIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEKPAVAAAIREHYLPNSAEGELPESKVGAV
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWEIPLDQLIAELYS
                     LNFASLTYDNQPAVMDFIRARVEKMMDKTIPKDIREAVLASSTFVVRLQLAASSAIFQ
                     KAKEADYKEAVENLSRVFNLAEKAEVTAIDEALFENDQEKALAAAVAGLELTEDMAGN
                     LDKLFALSPVIAAFFDNTMVMVDDAAVKANRLTLLKALADKASAVAVFNLLNSK"
     gene            20892..21149
                     /locus_tag="U1099_000491"
     CDS             20892..21149
                     /locus_tag="U1099_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEM4866837.1"
                     /translation="MEQAKIDRINELARKKKAEGLTEEELQEQAALREEYIEGYRRSV
                     RAHIEGIKVVDEDGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            21288..21659
                     /locus_tag="U1099_000492"
     CDS             21288..21659
                     /locus_tag="U1099_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866838.1"
                     /translation="MKKLLYFLLFLTYIACAYSTIFFLPFMYFSGIVGATGVGLGLSY
                     SLHQEVKKHPEKQLSEYLPWGSKRDKIMVVTILLFSILVSFIPDVNWHRGLAILLDIS
                     YVLLWQAMLIQFLRKYYFKNA"
     gene            complement(21681..23018)
                     /locus_tag="U1099_000493"
     CDS             complement(21681..23018)
                     /locus_tag="U1099_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEM4866839.1"
                     /translation="MNDLTKGKPIAVILQFAIPLLIGSFFQLAYNFADSMIVGHTLGK
                     DAFASVGSTASLIFLIIGFAQGVTNGLTIISAQRFGAGDLEGLKKSFVHGLFYASLIS
                     LLLTVSALAFLKPILVLMQTPVSIIDHSHAFLTAMFGGLTFTIFYNFLSSALRSLGNS
                     KTPLLALVIACFINIGLDFFFILVMNWGVFGAGFATILAQACSVLFLIFYIIHKVPHY
                     HIGLADLKLDRGNLKKHAQLAFPMGFQASIIAIGAMTLQFMVNQLGTDAIAAQAIALR
                     TDQLAMLPMVNLGLAIATFTAQNYGAKLYDRIREGVRHSLLLSIAWAIVFAVILILGN
                     RFFSGLFLPNASQTVLDLALVYYIINGSCYWIVASLFILRSFIQGLGKGFIPTLAGFG
                     ELIFRAAVAIIGMQYFGFYGTAAANPAAWIGSIIVLIPSSIIFMKKLKAGQSI"
BASE COUNT         6517 a         4514 c         5315 g         6780 t
ORIGIN      
        1 gcattttggg caggaatcca gacttccaag tcgtaggtct tagcagctga gaagcccata
       61 tcacctgtac agagggccaa aacacggtaa ggcaagccta gtttttgaag gatattttct
      121 gcgtttgcgg tcattttttc caattcatca taagactgtt ctggtgtggc aaatttgacc
      181 atttcaacct tgtggaattg gtgaaggcgg ataagaccac gggtgtcacg accagcagaa
      241 ccagcttcag aacggaaaga tgggctcatg gctgtgaagt agattggcag gtctttttct
      301 tccagaatct cgttgcggta gtagttagtc aacggcactt ccgcagttgg gatgaggacg
      361 aagttagtgt ctgccaactc aaaagtatct tccttgaact taggatattg accagtaccg
      421 aacatagagt cgtggttgac catgtaaggt gtgatgattt cttggtagcc ttctgcgata
      481 tgctcgtcca acatgaagtt gtagagggca cgttctaagc gagcacccaa gttcttgtag
      541 aaaaggaagc gtgcaccagt tacttttgcg ccacgttccc agtccaaaat atccaggtct
      601 tcgcccaaat cccagtgagc ttttggctcg aaagtgaatt cacgaggtgt tccccaacgg
      661 cgaacctcta cgttttcttc ttcatccgca ccaactggaa cagaatcgtg cggcgtattt
      721 ggaagaacgg tgatgatggc gtttaattct tcatcgattt ccaaaagctg agcgtctaag
      781 tttttcacat ccgcagacag tttctgcatg gcagcaatct tgtcgtcagc gttttcctta
      841 ttgcgttttg cttgggcaat ctccgcagaa acggtgttgc gctctgcttt taattcttct
      901 acagtaacca agatttcacg gcgttttgca tccaattcct tgatgtttgc caaggtttca
      961 gcagccacac cacgagtagc taatttggca gccactccgt caaaatcagt acgaatacgt
     1021 ttgatatcta gcatagatgt ctcctttaga ataaaaaagc acagaatgag agatgctgga
     1081 gcgatattta ccgcggttcc atccagcttc acattctgtg cacttgattg tattttgata
     1141 atttcataac ggtagaattt cacaactttt tcctatctgc tcacagcgac cgcagacttt
     1201 ctggaaggag ccagatgcta cttatccgtt tctactatta tacttgaatt tttatttttt
     1261 ggcaaataga ttcgaaattt ttctgccgat tgtttgcata agagtcggta atttcacaac
     1321 tttttcattg ccgatatgct cacgggcaat tttaagaatc ttaccagaat ctttagatgc
     1381 aaacaagaat cgacttttct ctgtataaat ttcaaaatga cggctgattg ctttgccaga
     1441 aacgtttgcg ccaatgctag tcatttctga ccaaggaatc tggatatagc gttcaacgtt
     1501 gctatctggg tagaattcaa gtgccttgtc accaattaga aatttaccaa ccttgccacc
     1561 aaagcccatg taggaaaccc ctgtggtttg tagttcaacg cttgtatttt gtgattgtgc
     1621 catagtgctc tctccctaga tattcattat atcataaaac cccgcaagag cggggtcctt
     1681 gattacataa ttccgaagaa acgagcaaga atacctacga tgaagagacc gataatcatt
     1741 gtgattggtg ttactttctt cttaagcaag tgcatgcaga ggaaagtaag gagaagtccc
     1801 atcaaaccag gaatcaagct gtccaactga ccttgtaatg tttgagcttg agttggttca
     1861 aggctaagtc cgctaagagc ttgaccaaga atacctttca actcagcacc agtaacagta
     1921 ccttctggga agacgatgta agcaccttct gacaattgtt tagcaggaag atctacagcg
     1981 aaagtaatag atacccaacg ttgaacaaga acggcaagga tgaacatacc aaggattgaa
     2041 gcacctttag tgatgtcttg aaggatacca ccagacatgt ctttagtgat ttcgctacca
     2101 gctttgtaac caaattcttg tgtataccat aggaaggcca tacgaatgat gttccagcca
     2161 aggaagaaga tgattggtcc catgatattt ccagatagtg caagtgaagc accaagagca
     2221 ccaaggattg gacgaacagt aaaccagaag actgggtcac cgataccagc aagaggtccc
     2281 atcataccga ttttcacacc ttggatagct gtgtcatcga tttcagcacc attagcacgc
     2341 tcttcttcaa gggcaagagt tacaccaagg attggtgaag caacgtatgg gtgtgtgttg
     2401 aagaattcca agtggcgctc aagagcagca gcttgatctt cttttttagt gtaaagtttt
     2461 ttgatagctg ggatcattgc ataagcccaa cctaagtttt gcatacgctc atagttccat
     2521 gaaccttgaa ggaaggttga acgccaccaa acttttttac gatcgcttac ggaaagttga
     2581 attttttctg acatgatgaa tctcccttct cttagtagtc ttcaaggatg tcgccgattg
     2641 ggtcgttaga tgaagcacca ccgttacctg atccgccttg ttttgacaag ttgaggtaga
     2701 taagagcgat tgcaacaccg ataacaccaa gtgcgataag tgtcaatttg ctgatagcag
     2761 caaatgcaaa accaagtgcg aagaatggcc atacttcacg agttgccatc atgttgataa
     2821 ccatagcata acctacggca acaaccatac caccaccaac ggccatacca cctgacaacc
     2881 aagctggcat agattcaaga actgcttgaa cagcttctgc aggaattgca agaagaagag
     2941 ccgcaggaac tgcgatacgc aaaccttgaa ggagaagagc aaacaagtgg taacgttcta
     3001 cagcagcgaa gtttgcatct ttagcagcat tgtcagcacc gtgtacaaga ccgactgaga
     3061 tagtacgaac gatcattgta aggaaaagac cagctacggc aagagggata gcagttgttg
     3121 ttgcaacggc aataccttca gttgagaagt cgccaccttt gatcatgatg attgcagcag
     3181 ctacagaagc aagagcagcg tcaggtgcaa cagctgcacc aatgtttgcc caaccaagtg
     3241 caatcatttg tagagaacca ccaagcataa cacctgcagc caagttacca gtcacaagac
     3301 cgataagggt acaagctacc aatggttgat ggaattggaa ttcgtcaagg ataccttcaa
     3361 gaccagcgaa gaaggcaacg atgacaacga gaattgcaga aataagtgta atatctgaca
     3421 tgttgtaatc ctttctatac taacagatcg atttattgaa cgtttgcctt gttgataagt
     3481 tcaaacaagt ctttcttcga atcgtttggt actttacgca catcaaattc aacgccaagg
     3541 tcacgcaatt tttcaaaggt tgcaacgtca tctttgtcca ttgacaatac gttgttaacc
     3601 attgttttac cagttgagtg agccatagaa ccaacgttca attctttgat ttcaacacca
     3661 ccttcaattg cttcaagagc ttcttgagga gtttcgaaaa gaatcaaggc gtgagtgtta
     3721 ccgaatcgag ggtctttaga aacctcaatc aatttcttga ttgggacaac gttcgctttt
     3781 acgttaccag gtgcagcttg tttgatgagt tgtttacgca attcatcttt ggcaactgta
     3841 tctgatacaa cgatgatacg atctgctttg gaagcaggag tccaaccagt cgccacttga
     3901 ccgtgcaaga gacgagtgtc gatacgggct aagttgattt tcaacttgcc atcaccgata
     3961 acagtacctg gagggatagc accttgtagt gcaacaggag cagcatcggc aacaggagta
     4021 gcttcagctg gttgaagttc ttctggaaga actttgatac catctttggc ttccttgata
     4081 atgtttgccg caacttcttc cacgccagca gcagcgttaa tcatacgctc tgtgtaagct
     4141 tggataagca ttggcaagtt tagaccggtg atgatagcaa acttacgatc tggattttca
     4201 ccagctacac ggcttgcttg gttgaatggt gaaccactcc aaaggtcagc caaaaccaaa
     4261 acttcatcat cggcatcgaa tgcgtccacg gcagcattta gcttagcgta taaatcatct
     4321 ggaccttcac ttggcataaa agtaacaact tgtacttttt cttgttcgcc aaagatcatt
     4381 gaacctgact gatgaatacc agcagcaaat tcgccatgac ttgcaataat gattccaata
     4441 ctcattattg atcctcctaa atttttcttg ttcatgtaaa aaccattgaa tgggctttca
     4501 tgttcgaact ataccattat aaaacgtttt cagtacattt gtcaagtagg tttgtgttaa
     4561 aagtgcaaaa tatttccaaa acttttcata atcatgtttc agaaaagtga taaaaatatg
     4621 caacgaatat gacaaaaacc tgacagttta tcaggttttt tgatgtctat aaaaataggt
     4681 tttctagttc acgtgcgaca ccatcttcaa aattagcaaa tgctgtcaat ctgtcggcat
     4741 gtggacgaag tgtatcgcta caatttttca tggcataggc tgttcctgct aggtctagca
     4801 tctcaacatc gttgtgctca tcgccgaagg caattaagtc gctaggagtg gcttggtaga
     4861 ggttaagaac atagctcaga gcggttgcct tattgacacc ttttgcacaa gtttcgagga
     4921 tatttaatgg tccgccccac gtattgattt ctaactcatc tttgaaatag gcattcattt
     4981 cttttgcaag ttcatactta tcagttgcac gagtctgcat aaggatggaa tgaggatcac
     5041 tagtgattaa ttcaggtttg atgagtgtat ctggagtaat ctgttcaacc cccatcagag
     5101 ccggatcaat cttatctaag ttcttttgag tgatgtagaa tttattcttg tattcaccag
     5161 caataaaatc tgcctcgaat gtttcttctt cttttagaaa ttcgagtagg tattttttgt
     5221 caattaaaat attttgttcc cattgccatt tctttccagg gatgtgaact aaagaaccgt
     5281 tgaaattaat catgggagta tccagttcta gttgttggta aaatttttca gccattcgat
     5341 aggggcgacc tgtggcgata agaacggtgt gtcctgcttg gcggaccttc tttatagttg
     5401 agatggtata atcagataat tggctatcat tattgagcaa tgtgccgtcc aaatcgatag
     5461 ctatgatttt tttcttcata tttaatagca ttcctttcta gagttgttat actagttttt
     5521 aatagtaaat aatgtttagc tagtagctag attgctgaga aattgtttta tgcgtcaaag
     5581 aatagtactt gtaggtaacc atatcttgaa ctaataactt ccagtataac aaaaggagat
     5641 aataaaagca aagaagcgat aaattttgta tagatttttg taagtctgca acgttcggga
     5701 tttgcgaatg tttaaagttg aaaatcaata aaataacgct ttcaagatta aaaaacttga
     5761 aattataatg ttttttgatt ataatgtttt tattgttcgt aaattaaaag gagaaatgaa
     5821 aaaaatggat caatttttta aattgaaaga acatggaacg actgtttcta cggaaattat
     5881 ggcaggcctt acgactttct ttgccatgtc atatatcttg tttgttaacc ctagtgtgtt
     5941 aagtgttgct ggaatgccag cgcaggctgt tttcttagcg actattattg ccagtgcagt
     6001 ttcaacgctt gttatgggat tatttgctaa cgtaccttac gccttggcac aggtatgggg
     6061 ttgaatgcct tctttactta tacagtggtt attggtcttg gttttacttg gcaagaagct
     6121 ttggcaatgg ttttcctctg cggtttgttt aacgttttta tcacagttac aaaggttcgt
     6181 aagagtatta tcaaggcgat tccagttagt ttacaacatg ccatcggtgg aggtatcggg
     6241 gttttcgatg cctatcttgg ttttaaaaac tctaatttaa tcaccttctt gacatctggt
     6301 tcagatatta ttactgtaaa tggtgttgct ccggcagatg caacagccga aacattctca
     6361 aacggtgttt tctcagtttt tgctggtggt ggtgtcgtac ctggtatttc aacctttact
     6421 gatccaagtg ttctcttgac agtgttcggt cttcttttga cagctgttct ggttattaag
     6481 aacgtgcgtg gtgcaatttt gattggtatt attgcaacga ctttagctgg aattccagca
     6541 ggtgttgtgg gcctgtctac catcgatttt gcaaataaca atatcggtac agcctttgcg
     6601 gagcttggta caactttctt agcggctttt gatggtctat cttcgctctt tgctgactca
     6661 agccgtctac cacttgtttt gatgactatc tttgcattca gcttgtcaga tacctttgat
     6721 acacttggta cctttatcgg tacaggtcgt aagactggta ttttctcaga agaagatgag
     6781 aaagctctcg aaaatggtac aggctttaac tctaagatgg acaaggcgct ctttgcagat
     6841 gcgattggta cctctatcgg tgcactattt ggtacttcaa atacaacgac atatgttgaa
     6901 tcggctgcag gtatctctgc aggtggtcgt actggtctga cagctgttac aactgccgtt
     6961 ctcttcttgc tatcaatttt gattttacca ttcattggaa ttgtgccaaa tgctgcaaca
     7021 gctccagcct tgattatcgt cggtgtcatg atggtttcat cctttcttga tgttgattgg
     7081 agccagtttg aagacgctct tccagccttc tttgccgcct tcttcatggc gctttgcttc
     7141 tctatctcat acggtattgc agccgcattt atcttctatt gcttagtaaa agtttcaact
     7201 ggtaaggcta aagaaattca tccaatcctt tggggtgcca cagctctatt tattctgaat
     7261 ttcatcattc tagccatctt gtaatctgtt tattatcctt gaactgatct tcatttgaag
     7321 gtcagttttt ttatttatga ttaaaatata tgggaatcaa tgtgatttat tcagcatatg
     7381 cggtattgaa cgttgtaatt tgaaaacgga tggagtttat ttttccattt cgttgcttct
     7441 ctgtctcctc ttagtgccat ttttactcct tgtgactgta ttttaaatgg ctgttcgtta
     7501 ttgtaagcaa gatgtctcat acctagacat ccgctggatt aatgtcatga tggggtgaaa
     7561 aaattatcat gtagtctttg ggagtcacag ggtatagaat aacccctgag cttgggttca
     7621 gggggagttt tctaactata atgtttgaat aacgaatgag ttgcttgatg gatggtgcgg
     7681 gccgtttcgg cattgttcat agtgtagaga tggataccag ccacatcatt ggtcactaag
     7741 tcaacaattt gatctacagc ataggccaga cctgctgcac gaagtgactc aggattgtgc
     7801 tcatattttt ccaggatagc tttaaattta cgtggtaagc gaatgttctc gcaggttttc
     7861 aagagacgaa gtgcctgatt cctgttgatg ataggcataa tacctgcgat aataggcact
     7921 tcgatgtctg ctagagaaca tttctcctga aagttataaa atgcttcgtt atcgaagaag
     7981 agttgagtta ccaaggttga gcaaccggca tcaactttct ttttcaaatt tttgatgtct
     8041 gagacagaat ttggtgattc tggatgtact tctggataac aggctccaat aatgtcaaaa
     8101 tgtggggctt cttccttgat aaaggaaatc aagtcagttg catactggaa gtcctcttta
     8161 gggggaagac cttcaataat atcgccacga agtgctaaaa tctgatgtac accaatttca
     8221 tcgagagaat gaagaacgga tcggaccttt tcttttgtca agtaagcagc tggcaaatgt
     8281 gcgatagttg gaatatgcaa ttcattgcga acatggttag ccaacttgac agtagtctct
     8341 tctacgttca aattgttatt gctgcatgta acactgataa aatgaggggc caaaccttgc
     8401 atctcatcga gagtttggat aatcttttca ttgcctactt ctggttttgg aggaaaaatt
     8461 tcaaatgaga gacttggggt ttgaccaatc ataaattaga gttcctttct ggctgtctta
     8521 gctgcttgag tcaagtggat aagactagct tttgtttctg attcgccacg agtcttgagt
     8581 ccgcagtctg ggttaatcca aactttttct ttcggtactt tagcaaggat ggcttgaatg
     8641 gtgtgtgcaa tttcatcaac ggcagggaca cgtggagagt ggatgtcata gacaccagga
     8701 cctacctgag tttggaagtt tttagccttg agttcatcta gaataacaag atttgaacgg
     8761 ctggcttcaa aggagataac gtctgcatcc atattgtcga tggcaggaat gatgtcagta
     8821 aattcgctat agcacatgtg agtgtgaatt tgtgtctctg gtgcaacagt tgagtgaact
     8881 aatcggaagg ctggaatcgc ccaatccaaa tattcactgt accagtcgct acgacgaagt
     8941 ggtaattttt cacgtaaagc tgcttcgtca atttggataa tcttgatacc cgctgcttca
     9001 aggtcaagaa cttcttcctt gatagcaagg gcgatttgaa gagttgattc tttaatagaa
     9061 atatcttcac gagggaatga ccagttgaga atggttactg gccctgtcaa cataccttta
     9121 actggtttgt ttgtacggct ttgtgcatag cttgaccatt taacagtgat tgggttcaag
     9181 cgcgtcacat ccccccagat gattggtggt tttaccccac gcataccata tgattggacc
     9241 catccgttct tactgaagag ataaccagaa aggttttcac cgaagtattc taccatgtcg
     9301 tttcgttcaa actcaccatg aaccaagaca tcgaagtcaa cttcttcctg ccatgcaatc
     9361 cattcgtctg tttgagcttt aacaaatgta tcgtattctt cttctgagat atttcctttg
     9421 cggaaagcca agcgtgtgct acggacttcc ttggtctgag ggaaggaacc gatagtggtt
     9481 gttggaagaa gtggtaagtt tagcttctcg cgttgaatgg cttcacgctc tgcaaagact
     9541 ggtaggcgtg tataatcggc gtctgtcagt cctgcaagac ggtctgcaag ggctgcatca
     9601 cgaccaacac gctcaagggc aaagagttcc ttgttagctg tgagagcaac ttcgccttga
     9661 ctgttacgga tggcatccaa atcacgcaat tcgctcagtt tttcaactgc aaaagcaaag
     9721 tggttgagga tagctggttc aaattcttca tttgctgttg tgaaaggtac atgaagaagg
     9781 gagcaagaag ttgttataac gacatgttca gctggaatag catccaaaac agccaggctc
     9841 ttttcgtagt tgttgcgcca gatattttta ccgttgacga taccagcgta gagggtcttg
     9901 ttagctggga aaccagttgc aacaagtgca gcagtttcct taccttctac aaagtcaaga
     9961 ccgatggcat caactggtag gctggtcaat tctgtgtaaa tatcacgaac atcaccgaag
    10021 taggtttgga tgagaacttg caaacctttc ttatctgcca agagtgtttg gtagatacgt
    10081 aggaaaaggg ctttttcttc agcagtcaaa tccttgacca agctcggttc gtccagctgg
    10141 attttttcag caccaagctc tgccaatttc tcaaagactt gtccgtaggc tgcaactaag
    10201 ctatcagcga aatcagtagc agttaaacct tcttcaaaat cagttaattg taagagtgtg
    10261 aatggaccaa tcaaaactgg acgagttgtg atgcccaagt tctttgcttc ttggaattca
    10321 tcgaaaatct tatgaccggc tagtttgatt tctgtatctt tttcaatagc tggaacgata
    10381 tagtggtagt tggtgtggaa ccattttttc atcggtctag cacgaacgtc ccctttttct
    10441 ccttgataac cacgtgcaag agcgaagtat ttttcaaggt cagtcaagtc aaggttctgt
    10501 actgctttag gtacgatgtt gaagagaaca gctgcatcca aaacattgtc atagtgggag
    10561 aagtcgttgc ttgggatttc agtaatgcct gcttctttaa caatattcca gtgcttagca
    10621 cgtaggtctt tagctacttc caaaagctct tcttgtggaa tttcatttct aaagtatttt
    10681 tcagtgataa attttaactc acggtgttct ccgatacgtg ggaagccgat aatagtagta
    10741 gtcatgtttt ttcctccaaa atatttctat gcccctatcc taacagaaaa aacgactctt
    10801 gtataattgt aaaaaattag agggagttat aacttttgac tataacagat tgaaagagag
    10861 ctactttttt gatatttctt ctatataaaa gcttgaaaaa agcctgttta tcaagcttga
    10921 cttttttatg ctggctccag tatagttttt tcttaagcgt ggtatagatt ttatctatga
    10981 aactgctaaa ggcaaactct tgtttatgag caattgttta taggtagttc tgatagttat
    11041 tttttatcaa gccataaaac ttctatatta gacagggcta ggtagccatg ctacaattga
    11101 aaaaatgaaa gaaaggggct ttttatggga aaagttatca ttggaatttc aggaaacgag
    11161 caagaatttc caactaaatc tggtagaact tatgtaaccg ttgcgagtga attggcagat
    11221 ggtgtgcgtc aggcaggtgg tgtaccaatg gttattccta tgggaactcc tgacttagcc
    11281 aaggactata tcaacatgat tgataagttg attttatcag gcggtcagca tgtagacccc
    11341 agtctatacg ggcaagagcg cttgattgat agtaatgatt acctcttgga acgggatgca
    11401 tttgagttag ctttgattga agaggccctt cggcaaggaa aacctatttt tgccgtttgc
    11461 cgtggtatgc agttgctcaa tgttgctcta ggtggtagtt tagagcagga agtggctcat
    11521 cattggcaag agggtattgc ggggacatcc catcgtttac aagtgaaacc taaaagtcga
    11581 atcggtcaac tgtttaccca agggagccct attaattcct tccatcagca acgaatcaaa
    11641 gaattggctc caggactggt ggcgacaggt ttggatccta gagatggtac gatagaagct
    11701 tatgaaagta gagggaacca agctttgttt ggtatccaat ggcattcaga atttttgtat
    11761 aatgattgta agcaacatcg agacctgttt cagtatttag tagatatatt ttaagagggg
    11821 tatccctctt tttattatca atgcttataa ttgcaaaggt atttgttata acgaaaagtt
    11881 ataactttct ataaaaaaag tcaattagtc agaaagaaaa ttctgtgtta agatagatac
    11941 agttctaatt taaggagatc attttatgaa attaaaaaaa ttgtttagtt tggcaacagc
    12001 tgctttatca gtaggtgtac ttgttgcatg tggtaactct tcttcaagtt cagattcatc
    12061 tgcaacaact gttcgagtag gtgtgatgag cttgagcgat tctgagcaag cccgttggga
    12121 taaagttcaa gaaattcttg gtgatgaagt gaaattggag ttcactcaat ttacagatta
    12181 ctcacaacca aacaaggcag ttgctgaaaa tgaagtagac atcaacgcat tccagcacta
    12241 caacttcttg aacaactgga atcaagaaaa tggtgaagac ttggttgcta ttgcggatac
    12301 ttatatcgca ccaatccgtc tttactcagg tacagctgat ggaaaaaaca agtacactaa
    12361 agtggaagaa attccagatg gtgcagaaat tgcggttcca aacgacccga caaacgaaag
    12421 tcgtgctctt taccttcttc aagcagctgg tttgatcaag gttggtgtat ctggtacaga
    12481 attggcaact attgctgata ttactgaaaa caagaaaaac ttgaaaatta ctgagttgga
    12541 tgcaagccaa acagcaagtt cacttagctc agttgatgca gcagttgtaa acaatacatt
    12601 tgtgttggaa gctggtttgg attacaaaaa tgccctttac aaagagcaaa aagatgaaaa
    12661 ttcaaaacag tggtacaact tgattgcagc tcgtagcgat tgggaaaaat cagagcaagc
    12721 agcagcgatt aagaaaatta tcgaagccta ccacacggat gaagtgaaaa aagttattga
    12781 agagacttca gacggtatgg atgagccagt ttggtaaaat gtaaataggt tgaggactgg
    12841 gcagaattgt tcagccctct atctgttttt atagagataa gaaaaaatct cttaaccaaa
    12901 gtcagaaatg tgggaggata tttgattttg gttagtagat ttaattgatt ttcattgagt
    12961 atgaggagaa agtatggcag atagtaaagt tcaagcattt gaaaatgatg cgattattca
    13021 aaactatttt gaaaagttga aggtcttgat ttcaaagaaa tctatttttg ctcagcagat
    13081 tggtttgttg gatgtggcga cttatttgaa agatatgttt gaagaggctg gggcagatgt
    13141 tgttttagac gacagctatg ccgcaccatt tgtcatggca acgtttaagg cttcagtgcc
    13201 agatgcgaaa acattgattt tctacaatca ttatgataca gtacctgcag atgccgatca
    13261 agtctgggag aagggcaacc ccttcgaatt gaccatttcc gatggctata tctatggtcg
    13321 cggggtagat gatgataagg gccatatcac agctcgtctg tcggccttga aaaaatatca
    13381 agctagacag aatggcgatt taccagtcaa tgtgattttt attatggagg gggcagagga
    13441 gtcagcttct gtcgacttgg acaaatacct ttcaaaatat aaagaatatt tgattggtgc
    13501 tgatttgctt gtctgggagc aggggcatcg caatagccta catcagctag aaattgctgg
    13561 gggaaataag ggaattgtga ccttcgatct tcaggtcaaa tcagctgact tggatattca
    13621 ttcttcctat ggaggtgtca ttgattcggc aagctggtat ttattatcag cacttcaatc
    13681 catgcgggct gcagatgggg aaattctggt agatggcatt tacgagcaag tgcaagagcc
    13741 aaatgagcgt gaattggcct tggtggaaga atttgcactg gctaccagtc agtctatgac
    13801 tgatatttat ggactaaccc ttccaacctt ggtagaagat cgtcgtgagt ttctgaagcg
    13861 tttgtatttt gaaccatcga ttaccattga aggcttgtca actggctatc ttggtcaagg
    13921 tgtcaagacc atcataccag ctcaggcttc tgctaagatg gaagtccgtc tggtacctgg
    13981 gttggagccg catgatgtat tagataagat tcgacagcat ttggacaaac atggttttga
    14041 caaagttgag cttgtcttca ctctaggaga gatgagctac cgaagtgata tgtcgcaccc
    14101 tgccattgtc aatgtgattg aattggccaa aaaattgaca ccagaaggag ttgctgtctt
    14161 gccgacgtca ccggggacag gacccatgca cacagtcttt catgctctag gtgtgccgat
    14221 tgcaggattt ggtctaggaa atgccaacag tcgcgaccat gcaggtgatg aaaatgtcag
    14281 catcgctgac tactatagcc atgttgaatt agtagaggag ttaattgcaa gttatgagta
    14341 aggaaatgat taagctagat aatattgatg taactttcca gcagaaaaaa cgtacaatcg
    14401 aagcggtaaa ggatgtgacc atccatatca atcaagggga tatttacggt attgtagggt
    14461 attctggagc agggaaatct acccttgttc gagtgattaa tttattgcag gtgccgacag
    14521 cgggtaaaat tactatcgat gaggatgtaa tttacgaggg agaaaaggtc acacttaccc
    14581 caactcaatt gcggagcaaa cgccgtgaaa taggcatgat tttccagcat ttcaatctga
    14641 tggcgcaaat gacggcagaa gaaaacgtag cctttgccct taagcattcg ggtttgagca
    14701 aggaagagaa gaaagaaaaa gtagccaaac tcttggactt ggttggactt tctgaccgtg
    14761 ctgaaaacta tccagctcag ctgtctggtg gtcaaaagca acgtgttgcc attgcgcgtg
    14821 ccttggccaa tgacccaaaa atcttgattt cagatgagtc aacttctgcc ttggatccga
    14881 aaacaactaa gcagattttg gcactcttgc aggaattgaa tgaaaaattg ggcctgacca
    14941 ttgttatgat tacccatgag atgcagattg taaaagatat ttgtaaccgt gtggcggtca
    15001 tgcaggatgg tcgcttgatt gaagaaggtt cagttttaga aattttctct catccaaaag
    15061 aagaattgac acaggatttc atcaaaacag caacaggaat tgatgaggcc ctggtgaaaa
    15121 tttaccagca ggatattgtt aagaatttgc ctgagaacag tatcttagtt caattgaaat
    15181 atgctggttc aaatacggat acggcgattg tcaatgattt gtataaattc tatcaagtat
    15241 ctgccaatat tctttatggc aatattgaaa ttttagacca tacgccagtc ggtgagatgg
    15301 tggtgatctt atcaggtgaa cctggtcagt tgcaccgtgc gattgaagca gtgacggagg
    15361 cgcgtgtgga agtgacgatt ttgaaaggag cgaactagat gttagaatgg attcaaacga
    15421 attttccaga tatttataaa ttgggctggg atggtcagac aggttggtta acacatttta
    15481 atttgaccct ctatatgacc tttgtttcct ttgcttttgg tggatttatg ggcttggtgt
    15541 ctggtttgtt cttggtcttg acaggtccac gtggggttat tgccaataag actgcctatt
    15601 ggattttgga caaggtggct tctatcttcc gtgccattcc ttttattatc ttgttggcgg
    15661 ctattgctcc ttttacgaaa attattgttg gaaaaaccat tggtacagag gcggctttgg
    15721 tgccgctcgc cctttcagtc tttccgttct ttgcccgtca ggttgaagtg gtcttatcag
    15781 aattggaccg tggtgtcatt gaggcggctc aggcttccgg tgctaccttc tgggatatag
    15841 tcctagtcta tctacgtgag ggtctgcctg acttgattcg tgtgacgacc tttgccttgg
    15901 tttccttggt cggctataca gccatggcag gagctatcgg agcaggtggt ttgggacaag
    15961 ttgccctatc atacggctac ctacggtata ataatgatgt gaccttttta gcaactcttt
    16021 tgattttagt tatcattttc gtaattcagt ttattggaga tttcttgacg agaaagatta
    16081 gtcatagata gttatttagc tgtgtttttt caaattttgg atagtccttg acttatttga
    16141 agcattttta tacaataaat ttaattaaat aaacactgcc accgggtagt catgcaacag
    16201 cattttatga acgtcgttag gtcttagaag atgttcatag ggtgctgatt tttctataaa
    16261 aggagtgaga atatgtcaca atttgttgtt gattcatctt tttggagtct atttccagat
    16321 gcaaaaattg gagttctatt attgaaagat tataaaacgc cggcgcaatc gcctgatgaa
    16381 ttagtgaaat tactggaaga aagcaacgga attgcccaga aatttcttac ggaagatacc
    16441 tttagtgaga atgaggtcat tcgtacatat cgtcaagcct atcaaaaatt taaaacaaaa
    16501 aaaggcgcgc gttctagtat cgaagcactt ttgaaacgtt caactagtga ccgtccagtc
    16561 acaacaattt ctccattagt ggacatttat aatgcggcga gtttacgttt tggtcttcca
    16621 tgtggcgctg aagatttaga tacatttgta ggcaatctcc aactaacggt cactgagggt
    16681 ggggatgaat tctatctcat tggtgatgaa accaataatc caactttacc aggtgaagtt
    16741 tgctataagg atgataaagg cgccgtttgt cgctgtttca attggcgcga tggtgagcga
    16801 accatgatta cggatgagac aaagaatgcg ttcttggtta tggagttggt caattccgat
    16861 agagtggaag atttggaaaa tgcccttgac ttcatcagtc agcaagcaga gaccttcttg
    16921 ggagtagtgc cagaaaaata tctactagat tgtgataggc ctggaatagc cttgtaggat
    16981 ttgtcagtgg gattgtgttc ggatagatag tggtctttct agacaaatgc tgatttttct
    17041 gctacaatac taagcatgaa agaaggttgt ttcaaaagcc tgtctagcga gcttgggaga
    17101 gtgaaagcca agccagtctt gaaataattg gcgctttccc agtcgtgacc atgtccgact
    17161 caatcagaat catacaaatg aagtaataaa ttagggtgga accgcgttct caacgcccct
    17221 atgtctgttg acaggggaag agaatgtggt tctttttgtc tgccgaccga gtatttcttg
    17281 tttttcatct caaggtgaaa aacagttact ataaagaaag gagaccaatg ttcaccttag
    17341 aagctatcaa tcacgggcat gagcagttta ctgggcctga ttttcccaag ctgattgcct
    17401 attttaagga cttgggtatg gtggaaaata ttgtggatat ccagtctgga caggttacct
    17461 atcgctcaca gacagggcag actctggaaa agacgggcta tcaagtaacg atacctgttt
    17521 cggaccaagc caatctggac cagtttgtga ctatcttacg caatcatcag gctggacaaa
    17581 cagattttcc caccttctgt caagaaacag cagaagcagg cgtttacaag tgggtcatcg
    17641 acctagaagc catgacctgt tcctatctgg accaggcgga gcaagctgtt tttgtggaag
    17701 ctgtaccgag tgtggagaac tagcaatttt gaaatataaa tagaaagagg atgttatgtc
    17761 aaaaaaactt acttttcaca tcgtcagtgg catagtctcc tttcagtcga attgccaaac
    17821 gctcatcgct atggcgactg agctagacgt tgtactagtt cgcctttcct agcggtatcg
    17881 gctaggaagg ctcacgtcgc aatcaattta tatagaaaga ggaaatatat gtctaaaaaa
    17941 ttaacattcc aggaaatcat cctgacctta caacaatttt ggaatgagca gggctgcttg
    18001 ctcatgcagg cttatgatac ggaaaaaggt gcggggacta tgagtccata cactttcttg
    18061 cgtgctatcg gtcctgagcc ttggaatgcg gcttatgttg agccttcgcg tcgtcctgcg
    18121 gatggtcgtt acggggaaaa cccaaaccgt ctctatcaac accatcaatt ccaagtggtt
    18181 atgaaaccat ctccatctaa catccaagaa ctctacttgg aatctttgga gcgtttgggg
    18241 attaatcctt tggagcatga tattcgtttc gttgaggaca actgggaaaa cccgtcaaca
    18301 ggttctgctg gtctgggctg ggaagtttgg ttggacggta tggaaatcac acagtttacc
    18361 tacttccaac aggttggtgg tttggcgaca ggtccagtca cagccgaagt aacttatggt
    18421 ttggaacgtt tggcatccta tatccaagaa gttgactccg tttatgatat tgagtgggct
    18481 gacggtgtca aatacggcga gatctttatc cagccagaat atgagcattc aaaatactcc
    18541 tttgaagtat cggaccaaga tatgctcctt gaaaatttca ctaagtttga aaaagaagca
    18601 gagcgggctt tggaagaggg cttggttcac ccagcctttg actatgttct caagtgctct
    18661 cacaccttta acctgctcga tgcccgtgga gccgtatctg taaccgagcg tgcaggctac
    18721 attgcccgta tccgtaacct tgcccgtgtc gttgccaaga cctttgtggc agagcggaaa
    18781 aaactcggtt tcccactttt ggatgaagca acacgagcag aattgttgaa ggaggacgca
    18841 gaataatgac aaagaattta cttgttgaac ttggtttgga agaaattcct gcctacatcg
    18901 taaccccatc catgcaccag ttgcgtgacc gcatggcgac ttttttgaca gacaatcgct
    18961 tggcttttga tagcattgat gtcttttcaa cgccacgccg tttggctgtt cgtgtccgtg
    19021 gtttagcaga ccagcagact gatcttacag aagatttcaa gggtcctgct aagaagattg
    19081 ccttggatgc agacgggaac ttcactaaag ctgcagaagg ctttgtccgt ggcaaaggct
    19141 tgacgacagc ggacatcgaa ttccgcgaga tcaagggtga agagtatgtc tacgtgacca
    19201 aacacgaagc tggtcagcca gctaaggcag tcttggcagg cattccagag gtcttgaagg
    19261 ctatgacctt ccctgtcagc atgaactggg cgtccaacaa attcgcctac atccgtcctg
    19321 tccacacctt gaccgttctt ttggacgatg aagcattgga tatggacttc ttggacatta
    19381 catctggtcg catcagccgt ggtcaccgtt tccttggaaa tgaaactgag attgcaagtg
    19441 cagattctta cgaggcagac ttgcgtgcac agtttgttat cactgacccc gcagagcgtc
    19501 aaaatatgat cgtcgagcaa atcaaggcta tcgaggacaa acacaacgtg acagttgaaa
    19561 ttgatgaaga tttgctcaac gaagttctca atctggtcga gtacccaact gccttcatgg
    19621 gttcttttga cactaaatat ttggaagtac cagaagaagt tttggtaact tccatgaaaa
    19681 accaccagcg ttactttgta gtgcgggata aggctggcaa gcttttgcca aacttcatct
    19741 ccgtccgaaa cggtaatgac cagtaccttg acaatgtcat taaaggaaac gaaaaagtct
    19801 tggtggcccg tttggaagat ggtgagttct tctggcgtga ggaccaaaaa ctcaagattg
    19861 ctgacttggt agagcgactc aaagttgtga ccttccatga gaaaatcggc tcactttacg
    19921 agcacatgga acgcaccaaa gttatcgcag aaaaactggc tgacttggca ggtttgtctg
    19981 cgggtgaaaa agcagatgtg gcgcgtgcgg cggacatcta caaatttgac ctcttgacag
    20041 gcatggtcgg tgagtttgat gaattgcaag ggattatggg tgagaagtat gcccttcttg
    20101 caggggaaaa acctgcggtg gcagcggcaa tccgtgagca ctacttgcca aactcagcag
    20161 aaggcgaatt gcctgagagc aaggtcggtg cggtcctggc actggctgat aagtttgaca
    20221 ccctcctatc cttcttctca gttggcttga ttccgtctgg ttcaaacgac ccttacgctc
    20281 ttcgtcgtgc aacacagggt atcgtgcgga ttttggaagc atttggttgg gaaattccat
    20341 tggatcagtt gattgcggag ctctacagct tgaattttgc tagcttgacc tatgacaacc
    20401 agccagctgt tatggacttt atccgtgctc gtgtagagaa gatgatggac aagaccattc
    20461 cgaaagacat ccgcgaggct gtacttgcca gctcaacttt tgtggtcaga ctgcaactgg
    20521 cagccagctc tgctattttc caaaaagcaa aagaggctga ctacaaggaa gccgtggaaa
    20581 acttgtcacg tgtcttcaac ttggctgaaa aggcggaggt gactgcgatt gacgaggccc
    20641 tcttcgaaaa cgaccaggaa aaagcccttg ctgctgcagt ggcaggcttg gagttgacag
    20701 aagatatggc gggcaacttg gacaagctct ttgctctcag cccagtcatc gcggccttct
    20761 tcgacaatac catggttatg gtagatgacg cagcagtcaa agccaaccgc ttgaccctct
    20821 tgaaagcctt ggcggataag gcaagtgctg tggcggtctt caatctcttg aatagtaagt
    20881 aggaggcatt catggaacaa gcaaaaatcg atcggatcaa tgaactggcc cgcaagaaaa
    20941 aagcagaagg cttgacagag gaggagttgc aggaacaggc ggctcttcgt gaggagtaca
    21001 tcgagggcta ccgccgtagt gtgcgtgccc acatcgaagg tatcaaggtc gtcgatgaag
    21061 acggcaatga tgtgacgcca gagaaactcc gtcaagtcca acgtgaaaaa ggcttgcacg
    21121 gacgtagctt ggacgatccc aattcctaag agtaagattg ctaaaaaaac aaggaaagac
    21181 taggttttct agtctttttt gtgcttttga gaacatatat ttacttgttc attcgattca
    21241 taaatggtaa aatgtatata gataaatagg ctcaggaggg ctttgtcatg aaaaaactac
    21301 tctattttct cttgtttctt acctacatcg catgtgcata cagcacgatt ttctttctgc
    21361 cttttatgta cttttctgga attgttgggg caacaggagt tggtctgggc ctaagctatt
    21421 ccctacatca agaagtcaaa aagcatcctg aaaaacaatt gtcagagtat ttaccgtggg
    21481 gatctaagag ggacaagatt atggtagtaa ccattctact tttctccatt ttggtcagtt
    21541 tcattccaga tgtgaattgg catagaggcc tggcaatcct cttagatatc agctatgtac
    21601 tcctttggca agctatgctt atccaattct tacgaaaata ttattttaaa aacgcatgag
    21661 agtttcatgc gttttctttt ctaaatactt tgtccagctt ttaatttttt cataaagatg
    21721 atggaacttg gaatcaggac gatgatgctt ccgatccagg cggcggggtt agcggcggcg
    21781 gttccataaa aaccgaaata ctgcatgccg atgatggcga ctgctgctcg gaaaatcagt
    21841 tctccaaagc ctgctagtgt tgggataaag cccttgccaa gtccctgaat aaagctacga
    21901 agaataaaga gggaagcaac aatccagtaa caagagccgt taatgatgta gtaaacaaga
    21961 gccaagtcca gcactgtttg gctggcattt ggcagaaaga gaccagagaa gaagcgattg
    22021 cccaagatga gaatgaccgc aaatacaatg gcccaagcaa tgctgaggag taatgagtgg
    22081 cgcactcctt ctcgaatacg gtcatagagc ttggcaccgt agttctgagc agtaaaggtt
    22141 gcgatagcca agcccagatt gaccatgggc agcatagcca actggtcagt acgaagggca
    22201 atggcctggg cggcgatggc gtctgttcct agttggttga ccataaactg caaggtcatg
    22261 gctccgatgg cgataatact ggcttgaaat cccattggaa aagcaagctg ggcatgtttc
    22321 ttgagattac ctctatccaa ttttaaatca gctagtccga tatgatagtg aggtaccttg
    22381 tggataatgt aaaaaataag aaagaggacc gaacaggctt gggcaaggat agttgcaaag
    22441 ccagctccga aaacgcccca gttcataact aagataaaga aaaaatccag accgatattg
    22501 ataaaacaag cgataactag agccagtaga ggtgttttag agtttccgag actgcgtagg
    22561 gctgaagaca ggaaattata aaaaatagta aaggtcaatc ctccaaacat ggccgttaga
    22621 aaggcatggg agtggtcgat gatactcaca ggcgtttgca ttaagaccaa gataggtttc
    22681 aaaaaagcta aggctgagac tgttagtaaa aggctgatga gggaagcgta gaagagtccg
    22741 tggacaaaag attttttaag cccctctaag tcgccagctc caaagcgttg ggcagaaata
    22801 atggttaatc cattggtaac accttgggca aaaccgatga ttaaaaaaat caggctggct
    22861 gtcgaaccga cacttgcaaa ggcgtccttt cccaaagtat gccccacaat catggaatca
    22921 gcaaaattat aggccagttg aaaaaaagat ccaatcaaca agggaatagc gaattgtaag
    22981 atgaccgcaa tcggctttcc tttggttaaa tcattcataa cgtctccttt tgacagatga
    23041 taagcccact agataaatga tagtctagta ggctttctag ggataaaata tttctcctga
    23101 aaacggagta taggcgtaaa tttgtt
//
LOCUS       DASFSW010000015        22833 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.015, whole genome shotgun sequence.
ACCESSION   DASFSW010000015 DASFSW010000000
VERSION     DASFSW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22833
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.015"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            106..945
                     /locus_tag="U1099_000494"
     CDS             106..945
                     /locus_tag="U1099_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM4866840.1"
                     /translation="MIIKRVLNHNAIIAQSNKNVDILLFGKGIAFGKKVGDAIPPSSI
                     EKSFLLKNSDNMNRFTELFIDVPIELVYVCEKIINLGKITLGNHFDEIIYINLTDHIN
                     SSIERHKEGVVVTNPLRWEIAKYYKEEFELGKKALSIIKKDLHVELPVDEAAFIALHF
                     VNANLENIFQESYRITGIIMKIEQIIQDYYSTEFNQDSIDYYRFITHVKLFAHRLVEG
                     NEYNDEDDVDLLELMKKKYPREYQCGTRVADFIRLEYDYLLSPSELVYLIAHIRRLTK
                     NLS"
     gene            956..2797
                     /locus_tag="U1099_000495"
     CDS             956..2797
                     /locus_tag="U1099_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM4866841.1"
                     /translation="MKYKNTALAILEAVGGEKNVLRATHCVTRLRLELKDENIVSDER
                     VKSISGVLGIMKKNGQYQIILGNDVANYYKEFTALGKFDSDSVQQVKKANVLEQVIEY
                     IAGSMTPLIPAMLGGGMIKVLVIVLPMLGLLKADSQSISFLAFFGDAPYHFMPIFLAY
                     SASQKLKVTPALAMSVAGILLHPNFVQMVSSGDPLHFLGAPVTPASYGSSVIPILIMV
                     WLMKYIEAVFNKVTPAITKSFLQPTLVLLVSGFIALVLVGPLGVIVGEGLSQLVEQMH
                     GVAGWLTLAVLGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVAFKSKNSNTKQIAFAAGFSALFAGVTEPALYGVTLKYKKPLYAAMIGGGLAGLFV
                     GLTGVKAYLFAVPSLIALPQFIYSEAASNITNAMIAAAISIIVTFILAYFLGIDEETS
                     TVNLEKVAPGISSRKNVFSPLSGQILPLEKVNDATFSKKMLGEGVAIIPKDGKVYAPF
                     DGAITSLFPTKHAIGLTSDEGVELLIHFGLETVELKGRGFVSHVSDGEKVEKGQLMLE
                     VDVEMLVAEGYDIVTPVVVTNTQEYLDVLLLSTKEEVNYADDLLAVL"
     gene            2812..4236
                     /locus_tag="U1099_000496"
     CDS             2812..4236
                     /locus_tag="U1099_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM4866842.1"
                     /translation="MSIFPDNFLWGGAVAANQVEGAYNEGGKGLSVQDVLPNGGLGEA
                     TQEPTEDNLKLQAIDFYHRYKEDIALFSELGFKVFRTSIAWSRIFPNGDEEFPNEEGL
                     QFYDELFDELLKNGIEPLVTLSHYETPLYLARKYHGWVDRKMIQFFEKFARTVFERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDLPNEELSKQDLYQAIHHELVASSLVTKIAHEI
                     IPDCKVGCMVLAMPTYPMTPNPEDVWAAHAYENLNYLFSDIHVRGYYPNYAKRYFKEN
                     GIKITFMEGDEELLKNNTVDFLSFSYYMSITQAADPSKYSSGRGNILGGLVNPYLEAS
                     DWGWQIDPIGLRLTLNRYYDRYQIPLFIVENGLGAKDQLVKDEAGGLTVHDDYRIQYM
                     QEHLLQVAEAIQDGVEVMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGSLTRY
                     RKKSFYWYKEVIQSNGSILGNSSK"
     gene            4993..5994
                     /locus_tag="U1099_000497"
     CDS             4993..5994
                     /locus_tag="U1099_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4866843.1"
                     /translation="MLKNKNLLATIVALTVMVVAALFLTQKEQNNSTNSTEKVKIGVL
                     QFVTHDSLDEIYKGIKAGLEEGGYTTTDNLEIDFMNAEGDQSQVQTMSKKLVDNGNEL
                     LIGIATPAAQSLANATTELPIIMGAVTDPVGANLVTDLKNPGGNITGVSDQTPVADAV
                     SLIKEITPDAKTIGVLYSSNEDNSKIQVAEFKAAAEEAGYTVLEYAVASSNEIAATVE
                     VASSKADVLFTPVDNTVASAFSTVVSVANKTKTPIFTSVEDMVEGGGIASVTLSQYDL
                     GVATGKMAAKILDGANPADTPVQIFNEGTVVVNQKVAKELGITLSDDVISKASKVIE"
     gene            6017..6883
                     /locus_tag="U1099_000498"
     CDS             6017..6883
                     /locus_tag="U1099_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4866844.1"
                     /translation="MIVSTVSQALLWAVLGLGIFVTFRILNFPDMTTEGSFPLGGAVA
                     VTLLTQGVHPILATLAAVLVGCLAGFVTGLLYTKGKIPTLLAGILVMSSCHSVILFIM
                     GRANLGLLNTKRLQDFLPFSEQVNGLLLGLGSLAVVIGALMFFLYTRFGQAFIATGDN
                     PDMARSFGINTSRMELLGLVLSNGIIALAGALIAQQEGYADVSRGIGVIVVGMASLII
                     GEVIYGNLTMLERFLAIVVGAIFYQFLILVVISLGINTSYLRIFSAMILATCLMVPEL
                     KNKLLKGASLSR"
     gene            6880..7638
                     /locus_tag="U1099_000499"
     CDS             6880..7638
                     /locus_tag="U1099_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866845.1"
                     /translation="MKAIVELKNATKVVTNGFDEERVILDNVNLTIYQGDFITILGGN
                     GAGKSTLFNVIAGTLPLTSGSVHILGEDVTNWPAEKRAKYLARVFQDPKMGTAPRMTV
                     AENLLIAKYRGEGRGLVPRRLAQQTEEFAQLLPRVGNGLEKHLDTPAGLLSGGQRQAL
                     SLLMASLKQPELLLLDEHTAALDPKTSQSLMQLTDELIETKGMTALMVTHQMEDALRH
                     GNRLIVMKNGQIIQDQTAEEKSKMKLEDYYKLFD"
     gene            7821..8306
                     /locus_tag="U1099_000500"
     CDS             7821..8306
                     /locus_tag="U1099_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEM4866846.1"
                     /translation="MDKLVFESLADLGMDYIVVEHPPALTTEEADRYIEGLEGVRTKS
                     MFLTNKKKTAFYLLIMDDQKQLDMDRFRDLVGANRIRMASSDSLMEKMHLPAGAVSVF
                     GLLHNVDKDIQVFFDKEILSEPILTFHPNVNTKTIFVKTEDVLRFVEEIGFSAKIVDL
                     G"
     gene            8312..8716
                     /locus_tag="U1099_000501"
     CDS             8312..8716
                     /locus_tag="U1099_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEM4866847.1"
                     /translation="MRKCLAICSLLCCLFLVGCRTGAAYQAEIEPMEQSELYTEKEIE
                     AAILVVQDYFDHHFNSCKLLTIGYSGDNEKEFDEWAEHYGAEEAIILTSSFKVAAEGA
                     EPTLEPNSTHTDWKWILVRNVGGKWEHKGHGY"
     gene            8972..10633
                     /locus_tag="U1099_000502"
     CDS             8972..10633
                     /locus_tag="U1099_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM4866848.1"
                     /translation="MSTIKIMALGGVRENGKNLYIAEVNEHIFVLDAGAKYPENEQLG
                     VDVVVPNFDYLEENKHRVAGVFLSHGHADAIGALPYLLEKVKVPVFGSHLTIELAKLL
                     VKGNNATKKFNDFHVINAETEIDFGDSVVSFFQTTHSIPESLGIVVKTDEGNIVYTGD
                     FKFDQAADKFYRTDFGRLAEIGNEGVLALLSDSANADSNVQVASMHEAGEEILNTIAD
                     CDGRVIVAAVASNIVRIQQIFDAAEATGRRVVLTGHDVENIVRTAIQLKKLRLVSERL
                     LVKPKDMNKYEDHELIILETGRMGEPLNGLRKMSIGRHRYVEIKEGDLVYIVTTPSIS
                     KEAVVARVENLIYKAGGTVKSITKNLRVSGHGNARDLQLMLNIMKPKYLFPVQGEYRQ
                     LDAHAKAALEIGMYPENIIIVKRGDVMAFEEGDFVHSGAVPAGDVMIDGNAMGDVGNI
                     VLRDRKILSEDGIFIVAITVNRREKKIISKAKVNTRGFVYVKKSKDILREASELVNTT
                     VENYFTKDSFDWAELKSAVRDDLAKFLFDQTKRRPAILPVIMEVK"
     gene            10698..11474
                     /locus_tag="U1099_000503"
     CDS             10698..11474
                     /locus_tag="U1099_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEM4866849.1"
                     /translation="MAIMKIEYHSEALDMSRQVTVLYPDRNRVENPDDNDIPVLYLLH
                     GMGGNQDSWLNRSTVERLVRYTNLIVVMVNTDKGWYTNTTYGMNYFDAIAVELPQILK
                     RFFPNMSDKREKNFIAGLSMGGYGTFKIAMMTNRFSHAASLSGALYFDFDNPAAAELG
                     SLSYWQGVFGDISDKQNPNNLLEIAKQSDKKTKFYAWCGEEDFLFEGHQKAVGELKEL
                     GFDIEASFGPGKHEWYYWNQQIEKVLAWLPIDFKLEERLS"
     gene            11540..12235
                     /locus_tag="U1099_000504"
     CDS             11540..12235
                     /locus_tag="U1099_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEM4866850.1"
                     /translation="MKLIGNILRWIWRTFWGLIWLSMMLLACGLGLLFYFQQEAAPQM
                     LQTLAQEVQLMVKGQSEVTIEEGVNTIKHLTTDTVNTADHGRWESNTATVYIETQNPT
                     FVAAYETAIANWNTTGAFTFVMTSDPNQADIIATEMNDGNTQAAGEANSTTNLLTNYY
                     SSVTVRLNSFYLLNDQYGYDMDRIIHTAEHELGHAIGLDHEDSQTSVMESAGSNHGIQ
                     QADIDAVLALYSE"
     gene            12246..12611
                     /locus_tag="U1099_000505"
     CDS             12246..12611
                     /locus_tag="U1099_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866851.1"
                     /translation="MLRTITNTIKRYPEQALLFLYNAGIFAWMQSTSHSIMEQIGIDS
                     NWFDKIPEPIKAWTGASLESMQTLLNSSAWGWLIVSMILMLVIRFVKGLIKFVIMLII
                     IGGGLYLLWQNKELVQSLV"
     gene            12662..14560
                     /locus_tag="U1099_000506"
     CDS             12662..14560
                     /locus_tag="U1099_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM4866852.1"
                     /translation="MIILSGNKIERSFAGEVLFNNINIQVDERDRIALVGKNGAGKST
                     LLKILVGEETATSGEISTKRDLSLSYLAQDSRFESANTIYDEMLHVFDDLRTTEKRLR
                     SMEEQMGSLSGSDLDQLMKTYDSLSEEFRLAGGFSYEADIRAILNGFKFDQTMWDMKI
                     SELSGGQNTRLALAKMLLESPELLVLDEPTNHLDIDTIAWLENYLVHYKGALIIVSHD
                     RYFLDKVATLTLDLTKHSLDRYVGNYSQFVELKEQKLQTELQNYEKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKMERLDKPTTGQKSANMTFQSDKTSGNIVLTVENAAV
                     GYDGDILSQPISIDQRKLDAIAIVGPNGIGKTTLLKSIIGALPFIKGEAKLGANVEVG
                     YYDQTQSALTPSNTVLEELWSAFPTTPEVEIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     KVLEISETGSTLYLGDYDYYLEKKAELEVEAQPDQLESTSQSAGAMDYQAQKENQKEQ
                     RKLARRIEQIEAEIETIENRLSELNQAMLETNDIGQLTDYQKEIDQLTSQQESLMEEW
                     EELSEQMG"
     gene            14569..14898
                     /locus_tag="U1099_000507"
     CDS             14569..14898
                     /locus_tag="U1099_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866853.1"
                     /translation="MIQQNTETLEDNLRFNKLSKLIQTKLSQYFETYPSAVQCQLVCF
                     DRDETRRKVLSYDIYSDFDKLFWVESIAKPISSYSYGHQIDKKDIDKLDREFILDTFL
                     GIDEERG"
     gene            14905..15732
                     /locus_tag="U1099_000508"
     CDS             14905..15732
                     /locus_tag="U1099_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM4866854.1"
                     /translation="MTKHQRFLNSDQFFACPHCGQALGLELNSLRCPNRHTFDIAKQG
                     YVNLAPQVKQSANYHKSSFENRQAFLEAGYYDHLYEALERKIAELGLRSVLDIGCGEG
                     FYSRKLAEKMDLDILAFDISKDSILLAAKSDRTKSVKWFVSDLTKLPIQDKTIDGILD
                     IFSPANYQEFARVLKAGGAILKLVPGPNHLKELRHLAKDQLRKESYDNQDIVDHFKSY
                     LGQVEQVLVSRTLPITAAHAQVLADMTPLFFQVDQSKLDLSQLTEITIEGVLLIGTI"
     gene            complement(15772..16557)
                     /locus_tag="U1099_000509"
     CDS             complement(15772..16557)
                     /locus_tag="U1099_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEM4866855.1"
                     /translation="MVYTSLKSPEKDYTYDLHIAHLYGDLMNTYGDNGNILMLKYVAE
                     KLGARVQVDIVSLTDEFDKNFYDIAFFGGGQDYEQSILAKDLPTKKDSLADFIENDGV
                     MLAICGGFQLLGQYYIEAGGRKIDGLGIMGHYTLNQTNNRYIGDIKIHNDEFNETYYG
                     FENHQGRTFLADNQKPLGKVVYGNGNNKEDGGEGLHYKNTFGSYFHGPILSRNANLAY
                     RLVTTALRKKYGQDIQLASYADILSKEVAEEYGDVKSKAEFEK"
     gene            complement(16557..17900)
                     /locus_tag="U1099_000510"
     CDS             complement(16557..17900)
                     /locus_tag="U1099_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEM4866856.1"
                     /translation="MKVNTALGILAGKSSQFILKKLGRGTTLPGKIALKFDKHILDSL
                     AKDYEVVVITGTNGKTLTTALTVGILQEAFGEITTNTSGANMITGITATFLSAKKNKK
                     GKKIAVLEIDEASLARVTDFIKPSLIVFTNIFRDQMDRYGEIYTTYQMILDGAAKAPQ
                     ATILANGDSPLFNSTNVINPVKYYGFATEEHEPRLAHYNTEGVLCPHCHQIIQYKLNT
                     YANLGSYICNNCGFCRPELDYKLTDLKEITNTSSTFAIDGQDYKINIGGLYNIYNALA
                     AVSVAAFFGVTPEKIKAGFDKSKAVFGRQETFKLGDKDCTLVLIKNPVGATQAIEMMK
                     LAPYDFSLSVLLNANYADGIDTSWIWDADFEQITQMDIPQVFAGGVRSSEIARRLRVT
                     GYPENQITETPKLEDIMALIEQSSSQHAYILATYTAMLEFRDLLAQRQAVGKEMK"
     gene            18082..18936
                     /locus_tag="U1099_000511"
     CDS             18082..18936
                     /locus_tag="U1099_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEM4866857.1"
                     /translation="MLNLNQLLDTGYWSSLIASPWTALLHLIDISIVVYLIYNFSKAI
                     AGTKIMTLIRGVFLFIIVQIIASLFGLQTIAWLINQVITYGVIAAVVIFAPELRAMLE
                     KLGRTTQIFGTNTVSAEEKLIVAFLKSVAYMSPRKIGALVAVEQAQTLQEYRATGIPL
                     NADISQELLINIFIPNTPLHDGAVIVKEDKVAVACAYLPLSESAGISKEFGTRHRAAI
                     GLSEVSDAFVFIVSEETGSISIARNGIFKHDLTLDEFEAELRATFISENVEKKSIWKR
                     LGGGKHER"
     gene            18926..19630
                     /locus_tag="U1099_000512"
     CDS             18926..19630
                     /locus_tag="U1099_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866858.1"
                     /translation="MNDKFKAVGHLALSIFLALLLFFYATTTNYKNSESAAQNTESET
                     YVHTLSNVPIEIEYDTNKYFISGLSSTVAVELRGSNRVLLQRESDESTRTFQVTADLR
                     ELSDGTQTVKLQLTNLPAGVSATLAPDAITVKIGKKVSKAFPVAGRVYSNQLAEGYSL
                     SKVSVNVDTVKVTTDEETMAKIDRVEAVAMDVSNLSENYSGTAKLQAVDSDGNVLPVV
                     LSQTEANMQIILTKTK"
     gene            19643..20992
                     /locus_tag="U1099_000513"
     CDS             19643..20992
                     /locus_tag="U1099_000513"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEM4866859.1"
                     /translation="MGKYFGTDGVRVEANVELTPELAFKLGRFGGYVLSQHETDVPRV
                     FVARDTRISGQMLEAALIAGLLSVGIHVYKLGVLATPGVAHLVKTEKASAGVMISASH
                     NPAQDNGIKFFAGDGFKLDDALEAEIEALLDAEEDTLPRPSAQGLGDVVEYPEGLRKY
                     QQFLVSTGTDLDGMKVALDTANGAAATSARQIFVDLGADLTVMAEKPDGLNINEGVGS
                     THPEKLQELVKETGSQIGLAFDGDSDRLIAVDENGDLVDGDRIMYIVGKYLADRGLLA
                     KNTIVTTVMSNLGFHKALDREGIEKAVTAVGDRYVVEEMRKEGYNVGGEQSGHVILMD
                     YNTTGDGQLTAVQLTKIMKETGKKLSELAAEVTIYPQKLVNIRVENSMKDKAMEVPAI
                     AAIIEKMEAEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRAEIGI"
     gene            21138..22682
                     /locus_tag="U1099_000514"
     CDS             21138..22682
                     /locus_tag="U1099_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM4866860.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGQLQPDEGKVEWSRYVTAGYLDQHAKLEKGQSVRDVLRTAFDELFKTEARIN
                     DIYMSMAEEGADMDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDYGMDKDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQLLTRYTGDYYQFQEVHAMKRAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMEIIELQAEKPKPSFDFKMARTPSRFIFQTTDLEIGY
                     DRVLTRKPLNLTFERNQKIAIVGANGIGKSTLLKSLLGIIPPLGGSVERGEYLELGYF
                     EQEVAGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLVLDEPTNHLDVDAKDELKRALQAYKGSILMVCHEPDFYEGWVDDVW
                     DFNELT"
BASE COUNT         6616 a         4209 c         5376 g         6632 t
ORIGIN      
        1 atgttatcgc tttaaggcga gcaggaacct atttaaatga atcgatacga tatatgtcga
       61 tttcttttaa gtaggttctt ttattttaag tttaaaggaa aatttatgat cataaagcgt
      121 gtattaaatc ataatgcaat catagcacaa agtaataaga acgttgatat attgttgttt
      181 ggtaaaggaa ttgcatttgg aaagaaggtc ggagatgcaa tcccaccttc ttcgatagaa
      241 aaaagttttt tgctcaagaa tagcgataat atgaatcgct ttacggaatt atttatcgat
      301 gttcctattg agttggttta tgtttgtgag aaaataataa atttaggaaa gataacactt
      361 ggaaatcatt ttgatgagat tatttacatc aatttgacag atcatatcaa ttctagtata
      421 gaaagacata aggaaggagt agtcgttacg aaccctctta gatgggaaat tgccaaatat
      481 tataaagagg agtttgaact ggggaaaaaa gcattatcca tcataaaaaa agatttacat
      541 gtcgaactcc cagttgatga agcagccttc atcgctttgc attttgtcaa tgcgaatttg
      601 gaaaatattt ttcaagaatc ttatcgtatt actggaatta tcatgaagat tgaacaaatt
      661 attcaagact attattctac agaattcaat caggattcta ttgattacta ccgattcatc
      721 acacatgtta aattgtttgc tcatcgtttg gtagagggga atgaatacaa tgatgaagat
      781 gatgtggatc ttttagagtt aatgaaaaag aagtatccaa gagagtacca atgtggaact
      841 cgagtagcgg actttattcg gttagaatat gattatctac tgagtccaag tgaattagtc
      901 tatttaattg ctcacattcg acgtttaaca aaaaatttat cataaggagt ctattatgaa
      961 gtacaagaat actgcattgg caatcctaga ggcagtcgga ggagaaaaga atgttcttcg
     1021 tgcaacccat tgcgttacta gattgcgact ggaattgaaa gatgagaata tcgtgtccga
     1081 tgaacgtgtt aaatccattt ctggagtact cggtattatg aagaaaaatg gacagtatca
     1141 aattatctta ggtaatgatg tagctaatta ctataaagaa tttactgcat taggaaaatt
     1201 tgattctgat tcagttcaac aggttaaaaa agcaaatgtc ttagaacaag tcatcgagta
     1261 tatcgctggc tctatgacac cacttattcc agctatgctt ggtggaggta tgataaaagt
     1321 tctagtcatt gtcttaccga tgctggggct tttaaaagcg gattcccaat ctatttcatt
     1381 tttagccttt tttggagatg caccatatca ctttatgccg atttttttgg cctactcagc
     1441 ttcacaaaaa cttaaagtta cacctgcttt agccatgtcg gttgctggta ttttgctaca
     1501 tcctaatttt gttcaaatgg tatcttcagg tgatccactt cattttttag gtgcccctgt
     1561 tacacctgct agctatggtt catcagttat tccaattctt attatggttt ggctaatgaa
     1621 gtatattgaa gctgttttca ataaggtcac tccagcaata acaaaaagtt tcttacagcc
     1681 aaccttggtc ctgcttgttt caggtttcat agctttagtt cttgttggac ctctcggcgt
     1741 tattgttggt gaaggattat cgcaattggt tgaacaaatg catggtgtag ctggttggtt
     1801 aactttagca gttcttggag caatcatgcc atttatcgtt atgaccggta tgcactgggc
     1861 ttttgcaccg attttcttgg cagcttcaat cgcgacacct gatgtactga ttctaccagc
     1921 gatgttaggt tctaacttgg cacaaggtgc cgcttctatg gcagttgcat ttaagagtaa
     1981 aaatagtaac acgaaacaaa tagcctttgc agctggattt tcagctttat ttgctggtgt
     2041 cactgagcct gctttatatg gtgttactct aaagtataaa aaaccattgt atgcagcaat
     2101 gattggtggt ggtctagcag gattatttgt tggtttgaca ggtgtaaaag catacctttt
     2161 tgcagtacct tctctcattg ctttgccaca atttatttac tctgaagcag cttcaaacat
     2221 tactaacgct atgattgctg cggcaatttc aattattgtc acatttattt tggcctattt
     2281 cctaggtatc gatgaagaaa cgtcaacagt taatttagaa aaagtagcac ctggcatttc
     2341 aagtagaaaa aatgtcttct ctcctctttc aggtcaaatt ctccctttag aaaaagtaaa
     2401 tgatgcaacg ttttcaaaaa aaatgcttgg agaaggagtt gcaattatcc ctaaggatgg
     2461 aaaagtctat gctccatttg acggggctat taccagtctt tttccaacaa aacatgcaat
     2521 cggtttaaca agtgatgaag gggtagagtt gcttatccac tttggattag aaactgtcga
     2581 gctaaaagga agaggttttg taagccatgt ttcagatggg gaaaaagttg aaaagggtca
     2641 actgatgctg gaagtggatg tagaaatgct tgtcgcagaa gggtacgata tagtgactcc
     2701 tgtagttgtt acaaatacac aggaatatct ggatgttctc ttgctttcga caaaagagga
     2761 agtcaactat gctgatgatt tattagcagt cttataatta gaggagggta tatgtcaatt
     2821 tttccagaca atttcttatg gggaggagca gtagctgcaa accaagtaga aggtgcatat
     2881 aatgagggag gaaaaggatt atccgttcaa gatgttcttc ccaatggtgg actgggtgaa
     2941 gctacccaag aaccaacgga agataatttg aaattacaag ctattgattt ttaccatcga
     3001 tacaaggaag atatagccct gttttcagag ttgggattta aggtatttag aacatcaatt
     3061 gcttggagta gaatatttcc gaatggagat gaagaatttc caaatgaaga agggctacag
     3121 ttttatgatg aactttttga tgaacttcta aagaatggta tagagcctct tgtaactctt
     3181 tctcactatg aaactcccct gtatctggca aggaaatatc atggctgggt ggatagaaaa
     3241 atgattcaat tttttgaaaa atttgctcgt acagtttttg agcgatacaa agataaggtt
     3301 aagtactggt tgacatttaa cgaggtcaat tctgtcttag aactgccttt tacaagtggt
     3361 ggaattgact tgccgaatga agaactttca aaacaagatt tatatcaggc cattcaccat
     3421 gaacttgtag cttctagtct tgttaccaaa attgctcatg aaattattcc tgactgtaag
     3481 gttggctgta tggtcttagc tatgccgacc tatccaatga cacctaatcc tgaagatgtt
     3541 tgggcggctc atgcctatga aaatttgaac tatttatttt cggatataca tgttagagga
     3601 tattatccta attatgccaa aaggtacttt aaagaaaatg gcataaagat tacttttatg
     3661 gaaggtgatg aagagttatt aaaaaataat acggttgatt ttctctcctt cagttattat
     3721 atgagtatta ctcaggcagc agatccatca aaatattcat ctggaagagg aaatatcttg
     3781 ggtggtttgg tcaatcctta tttagaagct tctgattggg gttggcagat tgatccaatt
     3841 ggtttaagat tgacactaaa tcgttattat gatcgatacc agattccttt attcattgtt
     3901 gaaaatggtt tgggagctaa agatcagcta gtgaaggatg aggctggtgg tttgactgtt
     3961 catgacgatt atcgaattca atacatgcaa gaacaccttt tacaagttgc ggaggcgata
     4021 caggatggcg tagaagtcat gggttatact tcttggggct gtattgattg tgtatctatg
     4081 tctaccgcac aattatctaa aagatatgga ctaatttatg ttgatagaaa tgatgatgga
     4141 agcggcagtc taactagata tagaaagaaa tcattttact ggtataaaga agttattcaa
     4201 tccaatggca gtatcttggg caatagctca aaatagactc ttaaggtatt aaaaagtttt
     4261 cagaaaattt aatcaaaagt attgacaaag tgattttgat accgtataat catctgtaat
     4321 cagaagaagt agtaaagcag aagtcttcag ggagcctgtg gtgctgagaa caggtggcag
     4381 agttttatga aatgggctga tgtgcattta tgtctatttt atgacgtcgt tagtcggtct
     4441 tgccgtacca ccagtactgc cttcgcccga ctgcctagtc ctaaaatgcc ctaaattcac
     4501 aaggattgtt tggcataaca tactttgcgt agactgtttt ggctgagtac gaggcaggtc
     4561 gacgcagctt atactagcgg tatagcaagg agagctaacg aagtaatcag ccaaaacagt
     4621 cacgtaaatg aacttaaaac aatgatagtt cggtccgcac accttacagt gcaactcctt
     4681 gttagaggag atgagatatg ggagggttct agacaattcc cataagtgag gtggcaccgt
     4741 gtcattgacg ccctcgcagg aagttttttc ctgcgggggc tttttccttg tctttgtaga
     4801 ttgcttttat attttgttac gattggcttg cctaacttga gttatgtctg cgcctccgtt
     4861 ccttatctaa aagcaatcta ctttgacaat tattaacaaa acgaggtaat catgatgaaa
     4921 cgatggcgat ggtgggataa tatattgatg acgtagaaat tgtatattgt gtattttacg
     4981 aggagaatga atatgttgaa aaataaaaat ttacttgcta caattgttgc ccttactgtt
     5041 atggtagttg ctgccctgtt tttgacgcaa aaagaacaga acaattcaac gaatagcaca
     5101 gaaaaagtca aaattggggt cctccaattt gtgacgcatg attcactaga cgaaatttac
     5161 aagggaatca aagcaggact tgaagaaggt ggttatacca caacggataa tttagaaatt
     5221 gattttatga atgcagaagg tgatcagtca caggttcaaa ccatgagtaa aaaattggtg
     5281 gacaatggca atgaattatt gattggtatc gcaacacctg ctgcacaaag tcttgccaat
     5341 gcaaccacag aattacccat tatcatgggg gctgtaacag atccagtcgg agccaacctg
     5401 gtgacggatt tgaaaaatcc tggtggaaat attacagggg tatctgacca aacaccagta
     5461 gcagacgcag tttctctcat taaagaaatt acaccagacg ctaaaactat cggtgtcctc
     5521 tattcttcaa atgaggacaa ttcaaaaatt caagtagcgg aatttaaggc agcagcagaa
     5581 gaggctggct atactgttct tgaatatgca gttgcttcta gtaatgaaat tgcagcgaca
     5641 gtagaggtcg caagtagtaa agcagatgtt ctctttacac cagtagacaa cacagttgcc
     5701 tcagcatttt caactgtcgt atctgtggca aataaaacca aaactccgat tttcactagc
     5761 gtagaagaca tggtagaagg tggaggtatc gcatctgtta cccttagcca atatgacttg
     5821 ggtgtggcaa ctggtaagat ggctgcgaaa attcttgatg gtgccaatcc agcggataca
     5881 cctgtacaaa tctttaacga gggtaccgtt gttgtcaacc aaaaagttgc taaagaatta
     5941 ggaattacct tgtcagatga tgtgattagt aaagcaagca aggttattga ataagagata
     6001 agagaaaagg aaaaatatga ttgtatcaac agtttcacag gctcttttat gggctgtact
     6061 aggtttggga atattcgtta cctttcggat tctgaatttt cctgatatga cgacagaggg
     6121 gtctttcccg cttggaggag cggttgctgt taccttattg acgcaaggag ttcatcctat
     6181 cttggctact ctagcagcag ttcttgttgg ctgtttggcg ggctttgtca caggcttgct
     6241 ttataccaag gggaaaattc ccactctcct agctggtatc ttggtcatgt cttcctgtca
     6301 ctctgtcatt ctatttatca tgggtagggc aaatctaggc ttactgaata ccaaacgttt
     6361 acaagatttc cttccttttt ctgagcaagt caatggtctc ttgctaggtc taggtagcct
     6421 agcggtggtg attggtgcct tgatgttctt cctctatact cgctttggac aggcctttat
     6481 tgcaaccggg gataatccag atatggctcg cagttttggt atcaatacaa gtcgaatgga
     6541 gttgctaggt cttgttcttt caaatgggat catcgctctt gcaggtgcct tgattgcaca
     6601 acaagaaggc tatgcggatg tgtcgcgtgg tattggtgtc attgtggtcg gtatggctag
     6661 tctgattatc ggtgaggtaa tttatggtaa ccttactatg ttagaacgat tcttggccat
     6721 tgttgtcgga gcgattttct atcagttctt aattttggtg gtcatttccc tcggtatcaa
     6781 taccagctac ctccgtatct tctcggccat gattctggca acttgcctca tggttcctga
     6841 gttgaaaaat aaacttctga aaggagccag tctgtcacga tgaaagcaat tgttgaatta
     6901 aaaaatgcca ccaaggtggt gacaaacggt tttgatgaag aacgtgtgat tttggataat
     6961 gtcaatctga ccatttacca gggtgatttt attaccattt tgggtggaaa tggggctggg
     7021 aagtcaaccc tctttaatgt cattgctgga accctaccct tgaccagtgg ttcagtccat
     7081 attcttggtg aggatgtgac caattggcct gcagaaaaac gtgctaagta tctggcacgt
     7141 gttttccaag atcccaaaat gggtacagcg ccacggatga cggtggcaga aaatctactg
     7201 atagctaagt accgtggcga ggggcggggc ttagtgccac gcagattggc ccaacagacg
     7261 gaagaatttg cccagctttt gccgagagtg ggaaatggtt tggaaaaaca tttggacacg
     7321 ccagcagggc tcttatcagg tggtcaacgc caggcattga gtttgttgat ggcgagtttg
     7381 aaacagcctg aacttttact cttggatgag catactgcag ccctcgaccc taaaaccagc
     7441 caatccttga tgcaattaac ggacgagttg attgagacca agggaatgac ggccctcatg
     7501 gtcactcacc agatggaaga tgctctgcgt cacggtaatc gcttgattgt catgaaaaat
     7561 ggtcagatta ttcaggacca aacggctgaa gaaaaatcca agatgaaact ggaagattat
     7621 tataagttgt ttgattaaga acctgtgtgg atagttcgca acttccaacc aagtttggtt
     7681 ttggctaaaa ctgaatctta tatcaaacag aacacttgtg aatacaggtc taaagtctgg
     7741 agtaatccag gcttttatta tcctttgaat ttttagttaa aaagtagtat aatatactaa
     7801 atatgtcaag aggtgctgtc atggataaat tggtttttga aagcttagct gacttgggaa
     7861 tggactacat cgttgtagag catccaccag ctctgacaac tgaggaagca gaccgttaca
     7921 ttgaagggtt agaaggagta cggaccaagt ccatgtttct gactaataag aaaaagactg
     7981 cattttattt actgatcatg gatgaccaga agcagctgga tatggaccgg tttcgtgatt
     8041 tggtgggagc caaccgcatt cggatggcct cgagtgatag cttgatggaa aaaatgcact
     8101 tgcctgcggg tgcggtatct gtttttggct tgttacataa tgttgataag gatattcaag
     8161 tcttttttga taaagaaatc ctttctgaac ccattctaac cttccacccc aatgttaata
     8221 cgaaaacgat ttttgtaaaa acagaggatg ttttgcgttt tgtagaagag attggctttt
     8281 cagcaaagat tgttgacttg gggtaacaaa gatgagaaaa tgtttagcta tttgtagtct
     8341 gttgtgttgt ctttttttgg taggatgtcg gacaggagca gcttatcagg cagagattga
     8401 accgatggaa caatcagaat tgtatacgga aaaagagatt gaggcggcta ttcttgtagt
     8461 tcaagattac tttgatcacc attttaacag ttgtaaattg ctgaccatcg gttacagtgg
     8521 tgataatgag aaggaatttg atgagtgggc ggagcactat ggtgcagagg aagctattat
     8581 cctaacttcg tcttttaagg tagcagctga gggggcagag cccaccttag agcctaatag
     8641 tacccatacg gattggaaat ggattttagt gcggaatgtt ggtggtaagt gggaacataa
     8701 gggacatggt tactaatgta tatgtagaac ttcgttgatt ttcagcggag ttttttgtaa
     8761 attttcttga aaaaatgtat aatagaagcg atagctattt aggtatttct atttaaaagt
     8821 tgcgttgatg gttgaatata attttgaacc aaagtgtgtc ttttgcaaat agagctccga
     8881 catagcagac tgtaattcga cttggaactc ccaaggaccg ttggtaagaa aaataaaatg
     8941 gacacctgtg ggatgtccaa aaggagtaaa gatgagtaca attaaaatta tggctcttgg
     9001 gggtgtgcgg gaaaatggaa aaaacctgta cattgccgaa gtgaatgaac atatttttgt
     9061 tttggatgca ggggcaaagt atcctgagaa tgaacagtta ggtgtggatg tagtcgtccc
     9121 aaatttcgat tatttggaag aaaataagca tcgtgtagca ggggtctttt tatctcacgg
     9181 gcatgcagat gctatcggag cgcttcctta tctcttggag aaagtgaaag tgccagtatt
     9241 tggttcgcac ttgactatcg aattggctaa gctacttgtc aaggggaata atgctactaa
     9301 gaaatttaat gatttccatg ttatcaatgc tgaaactgaa attgatttcg gagattcagt
     9361 ggtatctttc tttcagacga cccactctat tccagagagt ttaggaattg tcgttaagac
     9421 ggatgaggga aatattgtct acacaggtga ctttaagttt gaccaggcgg cagataagtt
     9481 ttatcgtacg gattttgggc gattggctga aatcggtaac gaaggtgtgt tggccctctt
     9541 gtcagactca gctaatgcag atagcaatgt tcaagtagcg agcatgcatg aggctgggga
     9601 ggaaattctc aatacgattg ctgactgtga cgggcgtgtt attgtggcag cagttgccag
     9661 caatattgta cgtattcaac agatttttga cgcggcagaa gcaactggtc gtcgtgtcgt
     9721 gttgacagga catgatgtgg aaaacattgt ccgaacagcg attcaattga aaaaacttcg
     9781 tttggtcagc gaacgcttgt tggtaaaacc aaaggatatg aacaagtatg aagaccatga
     9841 attgattatt ttagaaactg gtcgtatggg cgagccgtta aatggattgc gtaaaatgtc
     9901 tatcggtaga catcgctacg ttgaaatcaa agaaggtgat ctagtttata ttgttaccac
     9961 tccaagtatt tcaaaagaag cggttgttgc tcgtgtggag aacttgattt acaaggctgg
    10021 tggtaccgta aaatctatca caaagaatct tcgcgtctct gggcatggga atgctcgtga
    10081 tttgcagctc atgctcaaca tcatgaagcc taaatatctt ttcccagttc aaggggagta
    10141 tcgtcaattg gatgctcacg ctaaggctgc gcttgaaatt ggtatgtatc cagaaaacat
    10201 tatcatcgtt aagcgtgggg atgtcatggc ttttgaagag ggggattttg tccatagcgg
    10261 agcggttcct gctggtgatg tcatgattga cggaaatgcc atgggcgatg ttggaaatat
    10321 cgtccttcgt gaccgtaaaa tcctatcaga agacggtatc tttatcgtgg ctatcactgt
    10381 caaccgccgt gagaaaaaaa tcatttctaa agccaaggtc aatactcgtg gttttgtcta
    10441 cgtgaaaaag agcaaggaca tcttgcgtga ggcatcagaa ttggtgaata caacggtgga
    10501 aaattacttt acaaaggata gctttgactg ggcagagctt aagagtgctg ttcgcgatga
    10561 tttagcaaaa ttcctctttg accaaactaa acgtcgccca gctattttgc cggtaattat
    10621 ggaagtcaag taagctgatt tatatacata agaagagccg agggaaactc aggctcttta
    10681 ccataaggag aagatttatg gcaataatga aaattgaata ccattcagaa gctctagata
    10741 tgtctagaca ggttacggtt ttatatccag atcgaaatcg tgtggaaaat cccgatgaca
    10801 acgatattcc tgtcctatat cttttgcacg gtatgggagg aaatcaggat tcctggctga
    10861 atcgttctac agtggaacgg ttggttcgtt ataccaatct tatcgtggtt atggtcaata
    10921 cagataaagg ttggtatacc aataccactt acggtatgaa ttacttcgat gccattgcag
    10981 tagagttgcc tcaaattctc aaacgatttt tcccaaatat gtctgacaag cgggagaaga
    11041 attttatcgc tggtttgtct atgggtggtt atgggacctt caagattgcc atgatgacca
    11101 atcgtttttc acacgcagct tcgctttcag gcgcattgta ttttgatttt gataatccag
    11161 cggcagcaga attgggaagt ctttcgtatt ggcaaggagt atttggtgac atttctgata
    11221 aacagaatcc gaataatttg ttagaaattg ctaaacaatc cgataaaaag actaagtttt
    11281 atgcctggtg tggtgaggaa gattttctct ttgaagggca tcaaaaggca gttggggagt
    11341 tgaaagagct gggatttgat atagaggcaa gtttcggacc tggtaaacat gagtggtact
    11401 actggaatca gcagattgag aaggtattgg cttggttgcc aattgatttc aaattggaag
    11461 aaagactgtc ctagttttta agaaaagttt cagaaattat ggctatacta aaacctattt
    11521 ctagaaagag gtatagtcta tgaaacttat tggaaatatt ctacggtgga tttggcggac
    11581 attttggggt ctgatttggt tatcaatgat gttattggct tgtggtctag gtctattatt
    11641 ctattttcaa caggaagcag ctcctcagat gttacaaact ctagctcaag aagtgcaatt
    11701 gatggtcaaa ggacagtctg aagtgacgat tgaggaaggt gttaatacca ttaagcacct
    11761 gacgactgat acggtcaata cagctgatca tggtcgatgg gaaagcaata ctgcgactgt
    11821 ttacattgaa acccagaatc caacatttgt agctgcctat gaaacagcca ttgcaaattg
    11881 gaacacgaca ggggcattta cttttgtgat gacaagtgat ccaaatcagg ctgatattat
    11941 tgctactgag atgaatgatg gaaataccca ggcagcaggt gaggccaatt caacgactaa
    12001 tttattgacg aattattata gctcggtaac ggtgcgtctg aatagttttt atttattaaa
    12061 cgaccaatat ggttatgata tggatagaat tatccataca gctgagcatg aattgggcca
    12121 tgctattggc ctagatcatg aagatagtca gacctctgtt atggaatcgg caggttctaa
    12181 tcatggtatc cagcaggcag atattgatgc ggtccttgcc ttgtattcag aatagaaggg
    12241 aaaatatgct tcgaacaatt acaaacacaa taaaacgtta tcctgagcaa gctctgctct
    12301 ttctctataa tgctgggatt tttgcatgga tgcagtctac cagtcattcg attatggaac
    12361 aaatcggtat agatagcaac tggtttgata aaatccccga acctatcaag gcctggacag
    12421 gtgccagtct agagagtatg caaaccttac tcaattcttc tgcttgggga tggctgattg
    12481 tatctatgat attgatgttg gtcatccgct ttgttaaggg cttgattaag tttgtcatca
    12541 tgctcatcat tattggtggt ggtctttatc tgctctggca gaataaggag cttgttcaat
    12601 ccttggtata aatagaggaa tcaaatcagc cttgtgctga tttttttggt ataatatttt
    12661 catgattatt ctaagtggaa acaagattga acgctctttt gcgggcgaag ttttatttaa
    12721 caatatcaac attcaagtgg acgagcggga tcggattgcc cttgtcggaa aaaatggggc
    12781 aggcaagtct actctgctta aaatccttgt cggagaagag actgcgacaa gcggagagat
    12841 ttcaaccaag cgtgatttgt cactgtctta tctggcacag gacagccgtt ttgagtcggc
    12901 aaataccatt tacgatgaaa tgctccatgt ttttgacgac ctgcggacga cggaaaaacg
    12961 cctgcggtct atggaagagc agatgggcag cctatctggt agcgacctag atcaactgat
    13021 gaaaacctac gatagtctgt cagaggaatt tcgtctggca ggtggtttta gctatgaggc
    13081 ggatattcgt gccattttga acggctttaa gtttgaccag accatgtggg acatgaaaat
    13141 ctctgaactc tcaggtggtc agaatactcg acttgcactg gcaaaaatgc ttcttgaaag
    13201 cccagaactc ttggtcctgg acgagccgac caaccacctg gacatcgaca cgattgcttg
    13261 gttagaaaac tacctagtgc attacaaggg agccttgatt atcgtcagcc atgaccgcta
    13321 tttcttggac aaggtggcga cactgacact ggacttgacc aagcattcct tggatcgcta
    13381 tgtgggcaat tattctcagt ttgtcgagct caaagagcag aagttgcaaa cagaattgca
    13441 aaactatgaa aagcaacaga aggaaattgc caaactagaa gactttgtcc agaaaaatat
    13501 tgtccgtgct tcgaccacca agcgcgctca ggctagacgc aagcagttgg aaaagatgga
    13561 gcgactggac aagccaacaa ctggtcagaa gtctgctaac atgaccttcc agtcggacaa
    13621 gacttctggc aatatcgtct tgacagtgga aaatgctgcg gtgggctacg atggagatat
    13681 cctttctcag cccatttcta ttgaccaacg gaaactagat gccattgcca ttgtcggtcc
    13741 gaatggaatc ggaaaaacta ccctgctcaa atcaatcatc ggagctttgc catttatcaa
    13801 aggggaagcc aagctaggtg ccaatgtgga agtgggttac tacgaccaga cccagtcagc
    13861 cctaacacct tccaatacag tcttggagga gctctggtca gcctttccga ctacgcctga
    13921 agtggagatc cgcaaccgcc ttggagcctt cctcttctca ggagatgatg tcaaaaagtc
    13981 cgttgccatg ctgtcgggtg gggaaaaggc ccgtctgctc ctcgctaagc tatctatgga
    14041 aaacaacaac ttcctcatcc tcgacgagcc gaccaaccac ctggacatcg acagtaagga
    14101 ggtcctggaa aatgccctta tcgactttga cggcaccctg ctctttgtca gccacgaccg
    14161 ctatttcatc aaccgcgtcg ccaccaaggt gctcgaaatc tcggaaacgg gctccacgct
    14221 ctacttgggc gactatgact attatttgga gaaaaaggca gagctggaag tagaagccca
    14281 gcctgaccag ctagaaagta ctagccaatc agccggagcc atggactacc aagcccaaaa
    14341 ggaaaaccaa aaagagcaac gcaaactagc tcgccgcatc gagcaaatcg aagcagaaat
    14401 cgagacaatc gaaaatcgcc tgagcgagct caaccaagcc atgttagaaa ccaacgacat
    14461 tggccagttg accgattacc agaaagaaat cgaccaactg accagccaac aagaaagtct
    14521 tatggaagaa tgggaagaat tatctgagca gatggggtaa gaaataagat gatccaacag
    14581 aatacagaaa cactcgaaga taacttacga tttaataaat tatccaaact gattcagaca
    14641 aaattgtccc aatattttga aacctatcct tcagctgtcc agtgccaatt agtttgtttt
    14701 gatagagatg aaactcgtag aaaagtcctt agctatgaca tttattccga ttttgataag
    14761 ctcttctggg tagagtcaat agcaaagcct atttctagtt attcctacgg acatcaaatt
    14821 gataaaaaag atatagacaa actagatagg gagtttatct tggatacttt tcttggaata
    14881 gatgaagaaa gaggatagat tttcatgacc aaacaccaac gtttccttaa tagcgaccaa
    14941 ttttttgcct gtccacattg tgggcaggct cttggtcttg agctcaacag cctccgctgt
    15001 cccaaccgcc acaccttcga catcgccaag cagggctacg tcaatctggc accccaggtc
    15061 aagcagtctg ctaactacca caagtctagc tttgaaaacc gccaggcctt tttagaggca
    15121 ggctattacg atcatctcta cgaggctttg gagaggaaaa tagcagagct gggcttgcgg
    15181 tctgtcttgg acatcggctg tggagagggc ttttattccc gtaagttagc cgagaaaatg
    15241 gacttggaca ttcttgcttt tgatatttcc aaggattcca ttctcttggc agccaaatct
    15301 gacaggacca agtcggtcaa atggtttgtc agtgacttga ccaagttacc tattcaagat
    15361 aagaccattg acggcatctt ggacatcttt tccccagcca attatcagga atttgccaga
    15421 gtgctgaaag ctggtggggc aattctaaaa ttagtcccag gccccaatca cctcaaggag
    15481 ctccgccatt tagccaaaga ccaactccgc aaggaatcct atgataacca ggatattgtg
    15541 gaccatttca agtcttacct gggacaggtg gagcaagtgc tagtcagtcg aactctgccg
    15601 attacagccg cccatgctca ggtcttggca gacatgacac ccctcttttt ccaagtagac
    15661 cagtccaagt tggatttgag ccagttgacc gagattacga ttgagggagt gctcttgatt
    15721 ggaactatat aaaaaatgaa accgcctctg gatatcagaa gcggttttgc tttatttctc
    15781 aaactctgcc ttgcttttca catcgccgta ttcttcagcg acttctttgc tgaggatgtc
    15841 ggcgtagctg gcaagttgga tgtcttgtcc gtattttttg cggagggcag tagtgaccag
    15901 gcggtaggcc aggttggcat tgcgggataa gattggtccg tggaagtagc taccgaaggt
    15961 gtttttgtag tgcagacctt cgccgccgtc ttccttgtta ttgccattac cgtagacaac
    16021 cttgcccagc ggtttttggt tgtcagctag gaaagtacgc ccttggtggt tttcaaaacc
    16081 gtagtaggtt tcgttgaact catcattgtg aattttgatg tcaccgatgt agcggttatt
    16141 ggtctggttg agggtgtagt ggcccatgat tcctagaccg tcaatcttgc gaccgccagc
    16201 ttcaatataa tattgaccga gcaactggaa gccaccgcag atagctagca tgacgccatc
    16261 gttttcgata aagtctgcta ggctgtcttt ttttgtcggt aaatccttag caagaatgga
    16321 ttgttcatag tcctgtccac cgccgaaaaa ggcgatgtcg tagaaatttt tgtcaaattc
    16381 atctgtcagg gaaacgatgt ccacctggac acgagccccc agcttttcag ccacatactt
    16441 aagcatgagg atattgccat tgtcaccgta ggtgttcatc aaatcaccgt agaggtgggc
    16501 gatgtggaga tcgtaagtgt aatctttttc aggacttttt aatgacgtat agaccattat
    16561 ttcatctcct ttccaacggc ttggcgttga gccagcaggt cacggaattc gagcatagct
    16621 gtataagtag ctaaaatata agcatgctgg ctactcgatt gctcaatcaa ggccatgata
    16681 tcttctagtt ttggtgtttc agtaatctgg ttctcaggat agccagtcac acggaggcga
    16741 cgggcgattt cagacgagcg gacaccacct gcaaagactt gtggaatgtc catttgagtg
    16801 atttgttcaa agtcagcgtc ccaaatccag ctggtatcaa ttccgtcagc gtagttggca
    16861 ttgagaagga cggacaggct aaaatcgtat ggagccagtt tcatcatttc aatagcctga
    16921 gtcgcaccga ccgggttttt aatcaagacc agtgtacaat ctttatcacc cagcttgaag
    16981 gtttcttggc gaccgaagac tgccttggac ttgtcaaaac ctgccttgat tttttcagga
    17041 gttacaccga aaaatgctgc tacgctgaca gctgctaggg cattgtagat gttgtagagg
    17101 cctccgatgt tgattttgta atcctgaccg tcaatggcaa aggtagaaga agtattggtg
    17161 atttctttca agtctgtcaa cttatagtcc agctctggtc ggcaaaagcc acagttgtta
    17221 cagatatagc tgcccaagtt agcgtaggta ttgagcttgt actggataat ctggtggcaa
    17281 tgaggacaga gaacgccttc ggtattgtaa tgggccagac gaggttcatg ttcttccgtc
    17341 gcaaaaccgt agtattttac tggattgata acgtttgtcg agttaaaaag tggactatca
    17401 ccattggcca aaatagttgc ctgcggagct tttgcagccc cgtccaaaat catctggtag
    17461 gtggtataaa tctcaccgta gcggtccatc tggtcacgga agatattggt gaaaacaatc
    17521 aggctcggct tgataaagtc ggttacacgg gcaagacttg cttcgtcaat ctccaagacg
    17581 gcaatcttct tgcccttttt atttttcttg gcagacaaaa aggttgctgt aattcctgtg
    17641 atcatattgg caccgctggt attagtcgtg atttctccaa aggcttcttg gagaatccca
    17701 acggtcaagg cagttgttaa agtcttacca ttggttccag taataacaac gacttcataa
    17761 tctttagcca aactatctaa aatatgttta tcgaatttga gggcaatttt cccaggcaga
    17821 gtagtaccac gtcctagttt tttcaaaatg aactgggaac tttttcctgc tagaataccc
    17881 aaagctgtat ttactttcat gggactattt tatcataaag actggggatt ttctagaatg
    17941 gagtgtgttg gaacgtgata tattcctgca gtaaatctgt aatattttta acaggaaggt
    18001 cactattttc taaagaaaag gggaaaaaat atggtataat gagtacatta ctatgtatga
    18061 gttagaaagt atgagaatga tatgttaaac ctaaaccagt tactggatac agggtattgg
    18121 tcgagtttga tagccagtcc ttggaccgcg ctgctgcacc taattgatat tagtatagtt
    18181 gtttatttga tttataattt tagtaaggct attgcaggta ctaaaattat gacattgatt
    18241 cgaggggttt ttctcttcat tattgtccag attatagcga gtttattcgg tctgcagacg
    18301 attgcttggc tgattaatca agtaatcacg tatggggtta ttgcggcagt agttattttt
    18361 gcaccagagt tgcgtgctat gcttgaaaaa ctcggtagga caactcagat ttttgggaca
    18421 aatacagtga gtgcagaaga aaagttaatt gttgcttttc tcaaatcggt tgcttatatg
    18481 tctccgcgta aaatcggagc gcttgtggca gtagaacaag cccaaacatt gcaagaatat
    18541 agagcaacgg ggattccact taatgcggat atatcacagg aattgttaat aaatattttc
    18601 atcccgaata caccgttaca tgatggggct gtaattgtca aggaagacaa ggtagctgtt
    18661 gcttgtgctt atttgcccct gtcagagagc gcaggaattt caaaggaatt tggaacacga
    18721 catcgtgcag ccattggctt atcggaggta tctgatgcgt ttgtatttat tgtctctgaa
    18781 gaaactggca gtatctctat tgcacgaaac gggatattca aacatgattt gactttggat
    18841 gagtttgaag cagagttgcg agcgactttt atttctgaaa atgtagagaa aaagtccatc
    18901 tggaagcgat taggaggtgg caaacatgaa cgataagttt aaagctgtag gtcacttagc
    18961 cttatctatt tttctagcat tgttgctctt tttctatgca acaaccacca actataaaaa
    19021 ttcagaatca gctgcacaga acacggagtc agaaacctat gttcacacac tgagcaatgt
    19081 accgattgaa attgaatatg atacaaacaa gtattttatt tctggacttt cttcgacggt
    19141 ggcagttgaa ttaaggggct ctaatcgtgt tctcctacag agagaatcag atgagtcaac
    19201 tcggacgttt caagtgactg cggatttgag agagttgagt gatgggacac agactgtcaa
    19261 gctacaattg accaatttgc cggctggagt gagtgcgaca ctagcacctg atgcgattac
    19321 cgtcaagatt ggtaaaaaag ttagcaaggc attcccagta gcaggtcgag tatatagtaa
    19381 tcaattggca gaaggttaca gtctgtctaa ggtttcagtc aacgtagata ccgttaaagt
    19441 gacaacggat gaagaaacaa tggctaagat tgatcgggtc gaagctgttg cgatggatgt
    19501 gagcaatctg tctgagaatt atagtggtac agctaaatta caggctgtag atagcgacgg
    19561 caatgtcttg ccagttgtac tgtctcagac ggaagcaaat atgcaaatta tacttacaaa
    19621 aacaaaataa gaggttatga aaatgggtaa atattttggg acagatggtg tccgtgtaga
    19681 agcaaacgta gaattaacgc cagaattagc attcaaattg ggtcgttttg gtggttatgt
    19741 ccttagccag catgagacgg atgttcctcg tgtctttgtg gcgcgtgaca cacgtatctc
    19801 aggtcaaatg ttagaggctg ccttgattgc aggacttcta tcagtaggga ttcatgtgta
    19861 taaattggga gttttggcga caccgggtgt tgcccaccta gtcaaaactg aaaaagcaag
    19921 tgcaggtgtc atgatttctg caagtcataa cccagcacaa gataacggta tcaaattctt
    19981 tgctggtgat ggtttcaaat tggatgatgc attggaagcg gaaattgagg ctcttcttga
    20041 cgctgaggaa gacacattgc cacgcccatc tgctcaaggt ttaggagatg tggtagaata
    20101 tccagaaggt ctacgtaagt accagcaatt cttggtgtca actggtacag atttggacgg
    20161 tatgaaagtt gccctcgata cagccaatgg tgccgcagca acatctgcac gtcaaatttt
    20221 tgtagatttg ggagcagatt tgacagtaat ggctgaaaag ccagacggtt taaatatcaa
    20281 tgagggtgtt ggttcaactc atcctgaaaa attgcaagaa ctggtcaagg aaacaggaag
    20341 tcaaattgga cttgcctttg atggtgacag tgaccgcttg attgccgtcg atgaaaatgg
    20401 tgatttagta gacggtgacc gtatcatgta catcgttggt aaataccttg ctgatcgagg
    20461 tctattggcc aaaaatacaa ttgtgaccac agttatgtct aaccttggtt tccataaggc
    20521 tttggatcgt gaaggtattg aaaaagctgt gaccgcagtt ggtgaccgct atgtggttga
    20581 agaaatgcgc aaggaaggct acaatgtcgg tggtgagcag tcaggacacg tgattctgat
    20641 ggattataat accacaggcg acggccagtt gaccgctgtg caattgacca aaatcatgaa
    20701 agaaactggt aagaagttgt cagaattggc agcagaagtg accatttacc cacaaaaatt
    20761 ggtcaatatc cgtgtggaaa acagcatgaa agacaaggca atggaagtgc ctgctattgc
    20821 agctatcatt gaaaaaatgg aagcagaaat ggctggcaac ggtcgtatct tggtacgccc
    20881 aagtggtact gagcccctct tgcgtgtcat ggcagaagcg ccaacggatg ctgaagtcga
    20941 ttactacgtg gacaccattg ccgatgtggt ccgtgcggaa attggaattt aagcagaaat
    21001 caatcatttg ttaagatgat ttcattgtct tgaactacca gattgattag gtggctagta
    21061 gttccactca agactgcgag aataaaagta aaaactggag tttggttact ccagttcttt
    21121 gatgttgtga ggaaattatg agtattttag aagtaaagaa tttaagtcat ggttttggtg
    21181 accgcgcaat ttttgagaat gtctccttcc gtctcttgaa aggggagcat atcggtcttg
    21241 tcggtgccaa tggagaaggg aaatcgacct tcatgtccat cgtgacaggt caattgcagc
    21301 cagatgaagg taaggttgaa tggtcacgtt atgtaacggc aggttattta gaccagcatg
    21361 ctaaattaga aaagggtcaa tcagtccgtg atgtcttgcg gacagcattc gatgaactct
    21421 tcaagacaga agcccgtatc aatgacattt acatgtctat ggcagaagag ggtgccgata
    21481 tggatgccct catggaagag gttggtgaac tgcaggatcg tctggaaagc cgtgatttct
    21541 atacgcttga tgccaagatt gacgaagtag cgcgtgcgct tggtgtcatg gactatggca
    21601 tggacaagga tgtgacggag ttatcagggg gacaacgaac taaagttctt cttgcaaaat
    21661 tgctcctgga aaagcctgac atcttgcttc tggatgagcc gaccaactac ttggatgctg
    21721 agcatattga ctggctcaaa cgctacttgc agaactatga aaatgccttt gtcttgattt
    21781 cgcatgacat tcctttcttg aatgatgtga taaacattgt ctaccatgtg gaaaatcagc
    21841 tcttgactcg ctatacaggt gattactacc aattccaaga agtccatgct atgaagcggg
    21901 ctcagttgga ggcagcttat gagcgccagc agaaggaaat tgcagacttg caggacttcg
    21961 ttaaccgtaa taaggcccgt gttgcgactc gaaacatggc catgtctcgt cagaagaaat
    22021 tggacaagat ggagattatt gagcttcaag cagagaaacc aaagccatca tttgatttca
    22081 aaatggctcg aactccaagt cgctttatct tccaaacgac tgatttggaa attggttatg
    22141 accgtgtatt gacacgtaaa ccgctcaatc tgacctttga acgcaaccag aagattgcca
    22201 ttgtcggtgc caacggtatc ggtaaatcca ctcttcttaa aagtttgctg gggattattc
    22261 caccgctggg cggttctgta gagcgtgggg agtatttgga actaggctat ttcgagcaag
    22321 aagtagctgg tggcaatcgt cagactccgc ttgaagccgt ttgggatgcc tttccagccc
    22381 ttaaccaggc ggaagttcgg gcagccttgg ctcgttgcgg tctgacttcc aagcatatcg
    22441 aaagccaaat tcaagttctt tcaggtggtg agcaagccaa agttcgcttc tgtttgctta
    22501 tgaaccgtga aaacaatgtc ttggttctcg acgagccgac caaccacttg gatgtggatg
    22561 ccaaggatga actcaaacgt gcccttcaag cctacaaggg ctctatcctc atggtctgcc
    22621 acgagcctga tttctatgaa ggctgggtgg acgatgtatg ggattttaat gaattgactt
    22681 agattgaaag ctatcgaacg atagcttttt ctttgtgctt atactataat agaatgagca
    22741 tagtaaagga ggtaaaaaat gaaaagaaca gaacgaggcc agatttgcta ggcctctcta
    22801 gaggaagtaa aacggtctgg ggatagaccg ttt
//
LOCUS       DASFSW010000016        22158 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.016, whole genome shotgun sequence.
ACCESSION   DASFSW010000016 DASFSW010000000
VERSION     DASFSW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22158
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.016"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            379..639
                     /locus_tag="U1099_000515"
     CDS             379..639
                     /locus_tag="U1099_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEM4866861.1"
                     /translation="MDVKNLRKLKRIELYEIMLAQSEEIDCLRNQLEKIKTELADRRI
                     MISQSGSIAEASMKLTNIFEEAQKAADLYLYNVKNKIGDVHE"
     gene            632..1222
                     /gene="lepB"
                     /locus_tag="U1099_000516"
     CDS             632..1222
                     /gene="lepB"
                     /locus_tag="U1099_000516"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM4866862.1"
                     /translation="MNSSNLRGKKLPKTSDLQAVYQQAKYKKTFWETIRSTVFMLVVV
                     AAFAVLIAVLFLPILRIYGNSMKGTLNSGDIVVSVKSNDFESSDVVAFYYNNNILVKH
                     VIAEAGDWVDMDKQGNVYVNNQRLDEPYLANRDYSHTDIEFPYQVPENRIFVMGDNRK
                     ESIDSRNNAIGTVSNEQIVGKLVFKIWPLPELGWIE"
     gene            1419..6241
                     /locus_tag="U1099_000517"
                     /pseudo
     CDS             1419..6241
                     /locus_tag="U1099_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
     gene            6339..7775
                     /locus_tag="U1099_000518"
     CDS             6339..7775
                     /locus_tag="U1099_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM4866863.1"
                     /translation="MNKFKSLFMTVLAVLAMVLPTTVASAQEVDTNAGGTGTITVRNA
                     SQSQKYTIYKLFDATVTEDGSGISYKVPTGKILPADNPYFAVDSKGNITAKEGATVSS
                     EAFAAWAKNFGTEVTSATANDNSLVFTHLTFGYYFVTSSLGAVLTVDSTTPNATVIDK
                     NTTNPTIPDSNNGGGKKILSNGATTSETTAKIGDTINFQIKFNATNYVTKDRQTKQIV
                     SYAIEDSPTALAIDQNSVNVKVDGVDITAKISKTFDATGKMNLVLTWADAAASNKTIY
                     NSPAEVIITYSAVVTKDAKEGEATNSATIGYNTIDNPTTPPTPVDPDKPTETTKVTTH
                     RFTLKKTNNVGETLTGAEFKLYDAANNGTEIKVVKESDGVYRVAQADEQGVVIEAGEV
                     VIKGLKHSTTYYLEEMKAPNGYNILTERQSIEVKENNTAQANIVNKKGGVLPSTGAIG
                     ATLFYLVGSILLLVALVYTISKRRMNNI"
     gene            7889..8758
                     /locus_tag="U1099_000519"
     CDS             7889..8758
                     /locus_tag="U1099_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM4866864.1"
                     /translation="MIKWLKKKGPSFIMPLIFILGIGLLAYPSVSDYWNSFHQSEAIM
                     SYSDTVSDMTADQYKEIIDSARQYNQEKPLNWNVTASDIEAYNQELNFNKDGIMGYIE
                     IPKIDVKLSIFHGTDETVLETSIGHLEGTSLPVGGLGTHSVLSGHRGLPSARLFSDLD
                     KLREGDIFTIHVLNETLTYKVDQIRVVEPSDLSALTIEPQKDLLTLVTCTPYGINTHR
                     LLVRGYRIENVNGSALVTSDAIQIKAIFIAPFIATPILFVILIYIFITTSKTFRSWNR
                     NQVLDDFLVPKKF"
     gene            complement(8872..9149)
                     /locus_tag="U1099_000520"
                     /pseudo
     CDS             complement(8872..9149)
                     /locus_tag="U1099_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018958.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            9320..10669
                     /locus_tag="U1099_000521"
     CDS             9320..10669
                     /locus_tag="U1099_000521"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEM4866865.1"
                     /translation="MKMIDIVKNKKVLVLGLAKSGESAARLLDKLSAIVTVNDGKPFE
                     ENPTAQSLLEDGIRVICGSHPLELLDEDFALMVKNPGIRYDNPMVEKAIGKGIPVWTE
                     VELAYLVSDAPIVGITGSNGKTTTTTMIAEVLNAGNKPAKLCGNIGYPASSVAQTATA
                     EDTLVMELSSFQLMGTESFRPHIAVVTNLIASHIDYHGTFEDYVAAKWMIQGQMAAED
                     FVVLNFNQKEAKELAEQTKATVVPLSTLEVVDGAYLADGKLYFKGEYIMDADQIGVPG
                     SHNVENALATIVVAKLLGVDRQAIQESLSAFGGVKHRLQFVGEINGVSFYNDSKSTNI
                     LATQKALSGFDNSKVILIAGGLDRGNEFDELVPDLVGLKKMVILGQSAPRVQRAADKA
                     EVETIEALDIADATRKAFAIAEKGDIVLLSPANASWDMYANFEVRGDVFLQTFEELK"
     gene            10672..11736
                     /locus_tag="U1099_000522"
     CDS             10672..11736
                     /locus_tag="U1099_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEM4866866.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLIPRFLEEGWEVHYIGDGNGIEHE
                     QVVKSGLDVHFHSISTGKLRRYFSFQNMLDVFKVGFGVLQSVAIIAKIRPQALFSKGG
                     FVSVPPVIAANLLRVPVFIHESDLTMGLANKIAYKFATTMYSTFEQPASLTKVKHVGA
                     VTKVGKTNDKVTPIQLPEILSHFDKSLPTLLFVGGSGGAKVFNDLISQNSAALTERFN
                     IINLTGDSSLNQLDKNLYRVDYVTELYQPLMDLADVVITRGGSNTLFELIAMQQLHLI
                     VPLGRQASRGDQIENALYAEKKGYSKQIDESQLTFASLLVEVDELLKNKEFYIQNMTN
                     SNEIQSVDSFYNLLREDMGR"
     gene            11742..12836
                     /locus_tag="U1099_000523"
     CDS             11742..12836
                     /locus_tag="U1099_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEM4866867.1"
                     /translation="MTEKDSNVEESVLEVEQASQVELDSEQISPAEKESVLAEEKGLS
                     TDVSTDVDIPEMTAPDDEKSAFFEQWKARHQAYLAHKDAAEIQAVDEGQTEQENPEAK
                     KSKRVLFQGIERKTESLKSKKETGEKVQTLKVDIPSKVVWKAIPVLVTSLLLAALALY
                     FISPTSKKKQIEVVGNERLTAEQVENYSLISPDDYNVTVALHADAYAKNIKKNSSSVE
                     TATIKFQFPATFTIHIKEYAIIGYIQQQSQWYPVLSSGEIGGEPISQDSMPEGYTTIN
                     LSDKELIKELAIELGKIDAGIRSAIQTINLTPSKVTVDLLTFNMRDGNTVLVPLSEIS
                     QKVSYYTKIAAEVTVPTTIDMEVGIYRYAS"
     gene            12989..14368
                     /gene="ftsA"
                     /locus_tag="U1099_000524"
     CDS             12989..14368
                     /gene="ftsA"
                     /locus_tag="U1099_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEM4866868.1"
                     /translation="MARNGFFTGLDIGTSSVKVLVAEYIDNEMNVIGVSNVKSAGVKD
                     GIIVNIEVAAGAIKKAIEQAEEKSGIRIEKVNVGLPANLLQIEPTQGMIPVTTDSQEI
                     TDLDVENVVKSALTKSMTPEREVISFIPEEFTVDGFQGIKDPRGMMGIRLEMRGMLYT
                     GPRTILHNLRKTVERAGVQVENIVISPLALTRSVLNEGEREFGATIIDLGGGQTTVAV
                     MRGQELQYTNIYQEGGDYITNDISKVLTTSKSIAENLKYNYGIAYPQDASDKEKFTVE
                     VIGENAPVEVTERYLSEVIAARLRQIFDRVKQDLERTRALDLPGGIVIVGGGAILPGI
                     TELAQEVFGVNTKLFVPNQIGIRNPAFASVISFVEYVGELEDVENIAQHAVNGETTLR
                     HKPVEIPVARPRISQPIQREIVNELNTVDQVEEQVAPIYHDEEEEVRPAAKGNITDRI
                     RGLFGSMFE"
     gene            14394..15623
                     /gene="ftsZ"
                     /locus_tag="U1099_000525"
     CDS             14394..15623
                     /gene="ftsZ"
                     /locus_tag="U1099_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEM4866869.1"
                     /translation="MAFSFEAAASHGAVIKVIGVGGGGGNAINRMIEEGVAGVEFIAA
                     NTDVQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEALTNVLTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKNLGALTVAVVTRPFGFEGNKRGNFAIEGIEGLREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MENKGNALMGIGIGTGEDRVIEAARKAIYSPLLETTIDGAEDVIVNVTGGYDMTLTEA
                     EDASEIVNQAAGQGVNIWLGTSIDETMKDEIRVTVVATGVRQDTADKPARHRMEAVSP
                     RPSQRFDHSVASAPTRGAAQQTEAPKASAFGEWDLRRENLIRPTDTEPTSTVSVEKFT
                     MDHDEDELDTPPFFRNR"
     gene            15630..16301
                     /locus_tag="U1099_000526"
     CDS             15630..16301
                     /locus_tag="U1099_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEM4866870.1"
                     /translation="MNFQKNKDAIFSVVAKAAEKAGRPSESVKVIAVTKYVDSSIANQ
                     LVNTGITHIGENRVDQFLEKYNALADKQLTWHLIGTLQRRKVKDIIPFVDYFHALDSV
                     KLAQEIDKRATKIVKCFLQVNVSGEESKHGFAVSEIDAALAEISLLENIELVGLMTMA
                     PIDATDDELDEIFSKIQEIQEELATRNLPRMPFTELSMGMSGDFERAIAHGATYVRIG
                     SAFFE"
     gene            16318..16887
                     /gene="sepF"
                     /locus_tag="U1099_000527"
     CDS             16318..16887
                     /gene="sepF"
                     /locus_tag="U1099_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEM4866871.1"
                     /translation="MALKDTFKNLFNYFEVDEVNEVEEQADAYSMPNDRPKMRVANTT
                     VAPVREQQPKVETRREARSESQLQRLHERQQELMTNNNEKEIVKTTIDIKFPKRYEDA
                     PEMVNLLLDNASILIDFQYMSEQQARRCLDYLDGARSVLSGNLKKVSNTMWLLTPVNV
                     TVNIEELRNANAGTTTGAADSNFDFDIKR"
     gene            16892..17155
                     /locus_tag="U1099_000528"
     CDS             16892..17155
                     /locus_tag="U1099_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEM4866872.1"
                     /translation="MQILIFLLLKIVEIYSYLLLAYALLSWFPSLYTSSIGRLIQWLV
                     APILKPFKRFNLQFMGLDWTVMAAMIALNMGTRFLVQLLLLLA"
     gene            17158..17949
                     /locus_tag="U1099_000529"
     CDS             17158..17949
                     /locus_tag="U1099_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEM4866873.1"
                     /translation="MKNDKNLLQHFSREEREFVEKIMDMCQQVEDTYSYRLTHFLHPR
                     QDEIVCKIANYYQLQTFSSRDLVSTEHSRVIIAPTYYELDIKHFELTALHLSYARKFH
                     TLSHSQVLGTFLNQLGIKREYLGDILIDDERLIVFIDQKFGQIALQSITKVARVPVKG
                     KEEEWTTVRLPIGQEWRSKDVLVSSMRLDKLISVAFNLSRATANKLIEAGHVKLDYVL
                     TEQTSKLVEIGQLISVRRYGRVRLNEFLGFSKQGKIKLKLDIVKT"
     gene            17958..18647
                     /locus_tag="U1099_000530"
     CDS             17958..18647
                     /locus_tag="U1099_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEM4866874.1"
                     /translation="MALTALELKDKTFATKFRGYDADEVDDFLDIVTRDYEDLIRKNH
                     DQELELKNLRERLAYFDEMKESLSKSVLLAQDTAEKVKVAAEDQAANIIKQADYDAAT
                     LLHEAKDKANEILRNATDNAKKVVIETEELKNQTRIFHQRLKSTVESQLSLVNSSEWE
                     EILRPTASYIQTSDEAFRDVLHKALDEELPVEEESLDYTRQLTPEEIAELTRHAAAFE
                     SGESVVLSIEE"
     gene            18878..19321
                     /locus_tag="U1099_000531"
     CDS             18878..19321
                     /locus_tag="U1099_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866875.1"
                     /translation="MIRLELVNKDNFEAVLQVQLAPEDQRRVASVEYSLAQAWLYKDS
                     GMILPYAVVSGRKVVGFAMLSIETKDNSYYLWRLLIDKDFQNRGCGKEVVQQMIGKAK
                     ADPLCHKISINYVIGNHKMRYILEKFGFQPVGLDGQKMKMELIIK"
     gene            19332..22121
                     /locus_tag="U1099_000532"
     CDS             19332..22121
                     /locus_tag="U1099_000532"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEM4866876.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPTREPEWQKAWDEANLYARRQELN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPFVPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTKDYE
                     AAQIRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GLLDTDSYIVVWTTTPFTVTASRGLTMGADIDYVLVQPAGSDRKYVLAEALVDSLAAK
                     FGWESFEVISKHKGAEFEYIVTEHPWDTEVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGVKYDLEVAVTVNERGLMNEAAGPDFEGQFYDKVVPTVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDQIEATTFIPAWGKTRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVAALFEEHGSIIWWKSEAKDLLPAGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLAYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGMKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDTSNDVRVSMDILGQVSETYRKIRNTLRFLIANTSDFNPASDAVAYEELRSVDQYLL
                     VKFNKLVAQIREAYDNYDFMGIYKSVVNFVTLDLSAFYLDFAKDVVYIDGAKSLSRRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEHEAEEFVQLAEMPEVETFANEAQLLA
                     DWENFMVFRTQAQKALEEARNAKVIGKSLEAHLTAYVSAETKSFLESLNADLAQLLIV
                     SNLTVTTEAAPESAVLVEEVAFTVERASGEVCDRCRRIDTSVAERSYGATICDHCASV
                     VEENFAEAVAQGFEA"
BASE COUNT         6978 a         3981 c         4933 g         6266 t
ORIGIN      
        1 ctgttctagc tacctgcacc tcagttcctt gtctgaaagc taattcattc gaccacacaa
       61 ggcaatgagc tgccgccgcc aacttttgtc aacatcttag caattcgtgt tttacagtct
      121 gaaactgatc tctataattg acgggagtgg gaaggatatc catacattaa agtaattaaa
      181 gataaattaa ataactatat tagtcattca aacctaaatg agagggattt gttttataaa
      241 atattgctaa cagttttccc ttagacaaac tcatattact agacaataaa tggaaactat
      301 agtaaaatac caagtattgt tattaagttg tgccttaact taattttata atattttagt
      361 aaaagagagg aaacgcagat ggatgtaaaa aatcttcgga aattaaagcg aattgagtta
      421 tatgagatta tgttggctca aagtgaagag attgattgct tgagaaatca acttgagaag
      481 attaaaacag agttggctga tagaagaatt atgattagtc aatctggctc catagcggaa
      541 gcttcaatga aactgactaa tatttttgaa gaagcgcaaa aagctgcgga tctatatctc
      601 tataatgtga aaaataaaat tggtgatgtt catgaatagt tcaaacctca ggggaaagaa
      661 acttccgaaa acttccgatt tacaagcagt ttaccaacaa gctaaatata aaaagacctt
      721 ttgggaaacc atccgtagta ctgtatttat gcttgtagtt gtagctgctt ttgctgtgct
      781 gattgctgtc ctttttcttc ccattctaag gatttacggg aactcaatga aagggaccct
      841 gaacagtggg gacatcgttg tgtccgtaaa aagtaatgat tttgaaagca gtgatgttgt
      901 tgctttttat tacaataaca atattctggt caagcacgtc atcgctgaag ctggggattg
      961 ggtggatatg gataaacaag ggaacgttta tgtcaataat caaagattgg acgagccata
     1021 tttggcaaat agggattata gtcatacgga tattgagttt ccttatcagg taccggaaaa
     1081 tcgaatcttt gtcatgggag ataaccggaa agagtctatt gattcgcgaa acaacgctat
     1141 tggtacagtc tctaatgaac agattgttgg aaagttggta tttaagattt ggccactgcc
     1201 tgaattggga tggattgaat aaatgaaagt gaaaaaatag aaaggaggat cttgtgagag
     1261 actggttaaa tttagaaaaa atccaaggtt ggcgacgaaa gtacaagaag ctcgtgaata
     1321 tcattgcttt cctcgtagta tttgcaacga cctatgcttt gattctaccc gctatcacat
     1381 tagaccaaga aaaggctgaa caaactgctg ggattgaagt gcaagaaaca ccagtaacaa
     1441 cggaaattag taaattaaca gaaccgatgg tgacaactca gcaaactccg atagtgggta
     1501 gctctgctga tgaagttcaa tctcctgcga cagaagcggt tcctgaccct gctgagcaag
     1561 tgtcaacaga gtctgctagc aaaccccaga ccgaccaaga acggattact gagccaacta
     1621 ccatagttca caaaacgaat gattatgagg tgactgcaac atttgatgcg agtgctcaat
     1681 ttcccaaaga tgttgagttt aatgttagag aactagatag ccaatcagaa gaatatcgga
     1741 cacattatga aaaaaccaag gagaagcttg gagtaaaaaa cttgtatatg cccgattctt
     1801 tgatattcat ttttcatcac agggccaaga ggtcgagcct gctgccccta taaagattac
     1861 gattagaaac aaggaattag ttcaacggcc agatgatagt aaactgaaaa ttgtccactt
     1921 tgaagagaat agccaaatag aagttttaga ggccgaaacg aaggaaaatg aacaacaaat
     1981 ctccgagatc agttttgacg ctagccattt ttctgtttat ggagatattt tagcaaacta
     2041 ttataccgtc aaatttacct acactgacac agcgggtcaa gaacaagtca ttacgagttt
     2101 gattgataag actgtgggga cttcacttgg tagtttgcct gaagctcctt ttaaaaacgg
     2161 ttatgtattt tctcattgga ttaataaaga tactggtgat cggattgatg aaaatacggt
     2221 gataactgga gacctgactg ctgtagcagt atttaataat atcaatatct acaccattga
     2281 tgtcacgtat ttctaccaca atgtttctaa aaaccaagat attacgattg ataaggaaac
     2341 ggtgcaactg gaagcaagtg atgctccata tcaactgaca ccacctgctt ctactgaggt
     2401 gagcaacaaa gaggatacaa ctttaccagc agatgctatt tactaccctg aaaaaccact
     2461 tcttgagttt acagcagatc aactagagcg actggacgta gcagatggag tggatgataa
     2521 tcatattagc gaaagagtac aatatgttcc ttacaatgca gagtatgatg tcatttatag
     2581 gctaaagaat ttgacggggt caggttattc tgatatccag actgttcatg tccgtggggt
     2641 actgggttca actgttacac cgcaaattct aacctacaac tatgctgact ttgaacgtgc
     2701 tgatacagca aagattgaga atactagcgg ccaaaaattg tatgtttatt atacacggaa
     2761 aacctttact ctttcttata attccaatgg cggtagtaat attccccaac aacagggtct
     2821 atacgatagc cagattaaga tttctacaac tactccaaca aaagaggggt atacatttga
     2881 aggttggtat gataatcctg gactgactgg aaacaagatt acaggctctc tcaccttaaa
     2941 gaaagataca actttgtatg cgaagtggac accgaacagg gttcgctaca tcattgggta
     3001 ttacaaggaa gtctatgaca acaacactgg aacaacctcc tatgtatatg atagttcggc
     3061 agctggttgg ggtctggttg ggactactgt aaaaggaaca gattcaaata ttggacctcc
     3121 tccgccaacc caaacaacca ttggcagcat tggctacgag aaggatgtta ctaaaagtgc
     3181 tgcttcaagt attgaaattg cagctgatgg aacatctgtt ttgaaagttt actataaact
     3241 gaagcgctac acttttgaat ttttcttaaa tgataacaat gccaatatca ccataaatgg
     3301 taagacctac tctgatagga ataggtacat gatacgtgat gttgtgcttg gtcaagatgt
     3361 ctcaaagtta tggccagctt ctataacaaa tcctaaagaa gtatacgatg ataaagtcta
     3421 ctatgatagc tatggtaggc cgataagata cttttttgac tactggattt ttgggaatct
     3481 actcttcaaa acagatcggt tcgaagttgt tgagcctatg ttgccaacga gtggaacaac
     3541 cagtaagttc acagcatatt ggaataccca aggtacacag gctaacgttg aatattggtt
     3601 acaacaaccg gatggttctt ataagaagtc tgatatatat agtcaatctt tcctaaagac
     3661 tgcaggactg ctaccgaaag aaatttttgg atattcttat tatacagatg ctagttttac
     3721 tgaagcaggc attaggtatt acggaactca aggaaataca tatcgtttct actataaacg
     3781 ccaagccttc aaaattgact attattacaa tagtactggg ttagattcaa agagtaacat
     3841 tctctttgag agtgacatca atacagcaac ttataattac caaccaccac gttctactgg
     3901 agttgatgct gattatacat ggggtggatg gtatacagat tcgttattga cagagaagta
     3961 taactttaat aagatgcctg ccaataactt ggttctctat gctaagtgga ttgctccaaa
     4021 atttaaggtt acttttgata tcaacggtgg taatggaact gcccctgtcg atcaaagtgt
     4081 tgaaaaatac aaatacgcta aggcccctgc tgatccaacg agggaacatt acaatttcct
     4141 tggttggtct acagaagatg ggaaactcta tagttggagc gaccaagtaa ccaaggatgt
     4201 caaattgact gcgaagtggg aattgaaacc attaacttat actgttcgct atcttgaagc
     4261 aggtacaaat acgcaattgg ctgctgataa agtgattacg agtccagcgc ttgtattgaa
     4321 tcaagctatc acagaaaagg cattgggaat tacaggttat cgaccagata agagtagtca
     4381 aacaattaac ctgaatttta gagggaacga aataactttc tactatacac cgaaggtaaa
     4441 agatgttgtt tacaccatca agtatgtctt gaaggataac ccagctattg aggtagcacc
     4501 tacagttact cgtaaggttg atggaagtat catccgtgcg aaagaagctg ctgcagatgt
     4561 gaataaacag catttgcaga gtcagtctgg tgttactgct gaaatgctgg aaatggatta
     4621 ctatccaact gtaaatactc aaagtttgat tctaacttct aatgatgcaa ataatgtgat
     4681 tgtttttgaa tatgtcggat tcgatactca aacctttacg gtaaattatt tggatatgga
     4741 tggtaagaaa atcgatggtc aagaaccatt ggtagtttac cgaaagaaac caggtagttt
     4801 cgtggtagat cataagacta taaatggtta tacctatgat cattcgttag acagtgagaa
     4861 tgcagctgat aagacagtat accgtgtaag taagggtggt cacataacca ttgatctcta
     4921 ttataagaaa aacctaacac tgaccgcatt agataagtcc aaagtttatg atggcactgc
     4981 cctatcaagt tcgggtctat ctgatctaaa ccctgcttac agtgtagacc tgaaagcagg
     5041 agacaaatta gttgccatcg gctatgatgg tagccaaacc aatgcgggta gcagcgcaac
     5101 gacacctaaa cttgctaaca tcagcaatgc tcttggtgct gaccggacga atttctatca
     5161 cataacctat caagcgggta acttgagagt tgaccaacag ccagtcgtgg ttgtcatcaa
     5221 cggtgaaaag aaatcgaaaa tctatgatgg taaagcggaa gcggttggtt ataaggtgat
     5281 ggagataaga gatacaagtg gtctctataa agaaacagat atccatttta atggttcaag
     5341 ttcagagcag tcggtcaaga aaaaagatgc aggaatttat ccgctccttt taaataacag
     5401 attcacaaat accaatcaga atttccaagt agatttcttg atttctgatg gggaattaag
     5461 aatagataga cggcaactga ccataacatc tgattcagca agcaaggatt atgatggtag
     5521 tgagctccgt gctgacaata tcactgtatc agtacctgct ggtgttgact acacaggttt
     5581 tgtcgatgga gaaggagttg ttccaacctt cctaggcgga ccaaccgaac ctggcttcat
     5641 gcccaattac ttcacctatc agcctaaaga tggaactaat ctggctaatt atgagttgga
     5701 aacacaattt ggtcagttga aagtcaaaga agttatcaag attcaaaaga ctgacttggc
     5761 ttggcaggct ctgtcaggtg gtaagtttga acttaccaag tgggatggcc tcaattgggc
     5821 tcaggtagat ggtgcacaag aatttgcgat taccagcaaa gatggcagca atattcctgt
     5881 gggcttggaa cctggtcgtt accgactcca ggagttggca gcaccggatg gcttcattat
     5941 cctagatagc tatatctact tcaccatcaa ggataacttc aatgaagatc agacaatgtc
     6001 cttctacacg gtcaccctat ctgatgaaat gggcaatgat gccactccag aacgggccca
     6061 gttagaaaga acaacaggtg atgccagtca tcgtatcaag gttgctaatg agcaaggccg
     6121 agctctgcca agcactggcg gtagtggaca gaaatggttt atcctttcag gacttgttct
     6181 catggtcacc tcctatctga tacatctgtt tgttcagcgt aatcggaaaa tgaggtctta
     6241 gaactgagtt tccgcttgct ttaatttcgt gtcgcaatgc gagttgcagg atttcatgtc
     6301 tgcctctcca taataaaaaa ataaaaaagg aaaaaatcat gaataaattt aaaagtctct
     6361 tcatgaccgt gttggcagtg ctagctatgg tcttgcctac cactgtcgct agtgcgcaag
     6421 aggtagatac aaatgcaggt ggtacaggaa caattactgt gcgcaatgct tcgcaaagcc
     6481 aaaaatatac catctataaa ctctttgatg ctactgtaac agaagatggt tcaggtattt
     6541 cctacaaggt tccgactgga aaaatacttc cagcagataa tccctacttt gccgtggaca
     6601 gcaaaggtaa tattactgca aaggaaggtg ctactgtctc ttcagaagca tttgcggctt
     6661 gggcaaagaa tttcggtaca gaagttacaa gtgctacagc gaatgataac tctcttgtgt
     6721 ttacacattt gacatttggt tactattttg taacatcatc acttggtgca gtgcttacag
     6781 tagatagtac aacaccaaat gcaactgtta tcgacaaaaa taccactaat ccaaccattc
     6841 cagattcaaa taatggtggc ggtaagaaaa ttcttagcaa tggtgctaca actagtgaaa
     6901 caactgcaaa aattggcgat accatcaact tccaaatcaa gtttaatgct accaactacg
     6961 ttactaaaga tagacaaacc aagcagattg tttcttacgc tatcgaagat agcccaacag
     7021 ccctcgcaat tgatcaaaat tctgtcaacg ttaaagtgga tggtgtggat atcacagcaa
     7081 aaatttcgaa gacttttgat gcaacaggca aaatgaatct tgtccttaca tgggcggatg
     7141 cggcagctag taataaaacc atctataact ctccagctga agttattatc acctactcag
     7201 cagtggtgac taaagatgcc aaggagggag aagcaaccaa ctcagcgact attggttaca
     7261 acacaattga taatccaaca actcctccaa caccagttga tccagacaag ccaactgaaa
     7321 caacaaaagt gactactcac cgtttcactt tgaaaaaaac aaacaatgta ggtgaaactt
     7381 tgacaggtgc agagttcaaa ctctatgatg cagccaacaa tggaactgaa atcaaggttg
     7441 tcaaggaatc tgacggagtg tatcgtgttg cgcaggctga tgagcaaggt gttgtcatcg
     7501 aagctggcga agttgtgatt aaaggtctga aacatagcac gacctattat cttgaagaaa
     7561 tgaaagcacc gaatggttac aatatcttga cagaacgtca atccattgaa gtgaaagaga
     7621 ataacacagc acaagcaaat atcgtcaata aaaagggtgg tgtcttgcca agcactggtg
     7681 cgattggagc aactttattc tatctagttg gttcaatcct acttttggta gcattggttt
     7741 atactatttc aaaacgtcgt atgaacaata tctaattgtc catacaatac ctaatattta
     7801 gaatctatct tcggaagtgc aaaagcaaga gtggaagtat ttccgaagat atttctttgg
     7861 tctcaacaca aggaaaggag gggatttcat gatcaaatgg ctcaaaaaga aaggtccgtc
     7921 ttttatcatg ccattaattt ttatattggg gattggacta ttggcttatc catcggtcag
     7981 tgattactgg aattccttcc accaatcaga ggccatcatg tcctattctg acacagtatc
     8041 agatatgaca gctgaccaat acaaagaaat cattgatagt gcgcgccagt ataatcaaga
     8101 aaaaccctta aactggaatg tgactgcatc cgacattgaa gcctataatc aggaattgaa
     8161 tttcaataaa gatggcatca tgggctatat tgagattcct aaaattgatg tcaagttatc
     8221 tatatttcat gggacagacg aaacggtttt agagacttct attggacatc tagaaggaac
     8281 atcgttgcct gtaggaggtt tgggaactca tagtgtactt tcagggcatc gtgggctccc
     8341 atctgcccgt ctgttttctg atttagataa gttgagagaa ggcgatatat ttaccattca
     8401 tgttctcaat gaaacactaa cttacaaagt ggaccagatt cgagtggttg agccatcaga
     8461 tttatcggct cttaccatag aacctcagaa agacttattg accttggtaa cctgtacacc
     8521 ctacggtatt aatacacata ggctattagt tcgtggctat cgtattgaaa atgttaatgg
     8581 tagtgctttg gttacctctg atgctatcca aattaaagca atttttattg ctccatttat
     8641 cgcaactcca attctatttg taatcttaat ttatattttt ataacaacta gtaaaacatt
     8701 tcgttcttgg aataggaatc aagtgttgga tgatttttta gttcccaaga aattttgaac
     8761 tattgaaaat gtttcaaagt cgatttgact ctggaacatt tttctattat agacattaga
     8821 caatatggct gaatgaggta attgttcaac ttctaacctc ctgtcaactc aaatcaattg
     8881 cctagaccga cgattgaaac gacttctttc tagtaacgga ccacgaggaa agaggtggca
     8941 gatattgtag aaatgtcgtt gagatgtcat acctagttca gcttgtaaca aaagcaagac
     9001 aaggagtaat ctatctgaaa gcctacatta cgccgaagtt aaaaggaatc tgggcaatag
     9061 ttttggtaga aatgatgaga aatttgtgat aattggcgta cattccattg caaatgatga
     9121 gatttagcag tatactgtaa gtggctcatt tggactaccc ctctgtatgt ttcgtcgctt
     9181 acagtatagt ccatttgggc tttttattgt attttgaaat gaactagcgc tacgggtaga
     9241 gtttactttt ctgtcctctg gcttgaaaaa ggtcttcgta aagggtaaaa taaggtgtta
     9301 gaaagtaaag gtaattgaga tgaagatgat tgatattgta aaaaataaga aagtgctagt
     9361 gctaggttta gctaaatcag gggaatctgc agcccgtctc ctagataaat tgagtgctat
     9421 cgtcactgtt aacgatggaa agccttttga agaaaatccg actgctcaat ctttgttgga
     9481 agatggtatc cgtgttattt gcgggagcca tcccttggaa ttattggatg aagatttcgc
     9541 tttgatggta aaaaatccag gaattcgcta tgataatcct atggttgaaa aagcaattgg
     9601 caaaggaatt ccagtatgga cagaggttga attggcctac ttggtatcag atgctccaat
     9661 cgtggggata acaggttcga acggcaagac aacgacaacg accatgattg cagaagtctt
     9721 gaatgcagga aataaacctg caaaattatg tggaaatatt ggctatccag catcttcggt
     9781 cgctcaaact gcgactgcag aggatacctt ggtgatggaa ttatcatctt tccagttaat
     9841 ggggacagaa tctttccgcc cgcatatcgc cgttgtgacc aacctcattg ccagtcatat
     9901 tgattatcat gggacttttg aagactatgt agcagctaaa tggatgattc agggtcagat
     9961 ggcggctgag gattttgtgg tgctcaactt caaccagaaa gaagctaagg aattggctga
    10021 acagaccaaa gcaactgtag ttcctctttc tacgctggaa gtagttgatg gagcttactt
    10081 ggcagatgga aaactctatt tcaagggtga atacatcatg gatgctgacc aaattggtgt
    10141 tccaggttca cacaatgttg aaaatgcctt ggcaacaatt gtggttgcaa aattgttggg
    10201 agtagatcgc caagcgattc aggaaagttt gtctgccttt ggaggtgtca aacaccgctt
    10261 gcaatttgta ggtgaaatca acggggtttc cttctacaat gatagtaagt ctaccaatat
    10321 cttggcgact caaaaagctc tttcagggtt tgacaatagc aaggtcattt tgattgcagg
    10381 tggcttggac cgtgggaatg aatttgatga attagtccct gatttggtcg gactaaagaa
    10441 aatggtgatc cttggtcaat cagctccccg tgttcaacgt gctgcagata aggcagaagt
    10501 ggaaacgata gaagctctcg atattgcgga tgcgactaga aaagcctttg ccattgcaga
    10561 aaaaggagat attgtccttt taagtccagc caatgcaagc tgggatatgt atgccaattt
    10621 tgaagtgcgt ggcgacgtct tcttacagac ctttgaggag ttgaaataat catgaaaaaa
    10681 attgttttta caggcggagg gactgttggg cacgtgacgc tcaatctcct tttgattccg
    10741 cgatttttgg aagaaggctg ggaagtccac tatattggtg atgggaacgg tattgagcat
    10801 gagcaggttg tgaaatcagg tttagatgtc cattttcatt ctatttcgac tggaaaattg
    10861 cgccgttatt tctctttcca aaatatgttg gacgtgttta aagttggttt tggtgttctt
    10921 caatccgtgg ccattattgc taaaattcgt ccgcaagctc ttttttcaaa aggtgggttt
    10981 gtatcggttc cgccagttat tgctgctaat ttgctgcggg ttccggtctt tatccatgaa
    11041 tcagacttaa caatgggact ggccaataaa atagcctata aatttgcgac aaccatgtat
    11101 agtacttttg agcaacctgc tagcttgact aaagtaaaac atgttggtgc ggttacaaaa
    11161 gtggggaaaa cgaatgacaa agttacacca atccagttgc cagaaattct cagtcacttc
    11221 gataaaagtc tgccgaccct actgtttgta ggtggttcgg gcggtgctaa ggtctttaat
    11281 gatttaatca gtcagaatag tgctgctctg accgaacgat tcaatattat caatttgaca
    11341 ggggatagta gtctcaacca attagataaa aacctatata gagttgacta tgtgacagaa
    11401 ctttatcaac cgttgatgga tttagcggat gtcgttatta ctcgtggtgg ttccaatact
    11461 ctgtttgagt tgattgccat gcaacaactc catttgattg tgcccctagg acgacaggcc
    11521 agtcgagggg atcagattga aaatgctctc tatgcagaga aaaagggata ttctaagcag
    11581 attgatgaaa gtcaattgac atttgccagc ctgttagtag aagtagatga gctattgaaa
    11641 aataaggaat tttatatcca aaacatgaca aattctaatg aaattcagtc agtagatagt
    11701 ttttacaatt tgcttagaga agatatggga aggtaggtcc tatgacggaa aaagattcaa
    11761 atgtagaaga atcagtgcta gaagtggaac aggcatctca agtagaattg gattcagaac
    11821 aaatctcacc agccgagaaa gaatctgtat tagccgaaga aaaaggactt tctacggatg
    11881 tttctacgga tgtagacata cctgagatga ctgctccgga tgatgaaaaa agtgcctttt
    11941 tcgaacaatg gaaagcacgc catcaagcct accttgctca taaagatgct gcggaaattc
    12001 aggcagttga cgagggacag acagaacagg agaacccaga agctaaaaag agtaaaaggg
    12061 tcctttttca gggaatagaa agaaaaacag agagtctgaa atctaaaaag gaaacagggg
    12121 aaaaggttca aactttaaaa gttgatattc cttccaaagt agtctggaaa gctattccgg
    12181 ttcttgtgac gagtttgtta ctggcagctc tagccttgta ttttatttct ccaaccagca
    12241 agaaaaaaca aatagaggtt gtcggaaatg agcgactaac tgctgagcaa gtagaaaatt
    12301 atagccttat ctctccggat gattacaatg tgactgtagc tttgcatgca gatgcttacg
    12361 caaagaatat taagaagaat agttcgtccg ttgagacagc gacaatcaag ttccagtttc
    12421 cagctacctt taccattcac atcaaagaat acgcaataat aggctacatt cagcaacaaa
    12481 gtcagtggta tccagtattg tctagtggag agattggcgg tgaaccgatt tcacaggatt
    12541 ccatgccaga aggctacaca acgataaatt tatccgacaa ggaattaatc aaggaactgg
    12601 ccattgaatt gggtaaaata gatgcaggaa ttcgttctgc tattcagaca ataaatttaa
    12661 cgccaagtaa agtgaccgtg gatttgctga cattcaatat gagagatgga aatactgtat
    12721 tagtcccgtt gagtgagatt agccagaagg tgtcctacta tacaaaaata gcagcagaag
    12781 tgactgttcc aacaacaatt gatatggaag ttggtattta tagatatgcg agctgaaata
    12841 gataattttg taaagaaaaa gtaataaaaa aatcctttaa aaacaccata attcgatata
    12901 ttatggtata atgaatgatg actaattata ctatttttca aaatggaaga tgatttgtta
    12961 aagttggaag taagagagga ctgatagaat ggctagaaac ggctttttta cgggattaga
    13021 tattggtaca agctccgtta aagtcttggt tgcagaatat attgataacg aaatgaatgt
    13081 gattggggtc agcaatgtaa aaagtgctgg cgtcaaagat ggaattattg taaatattga
    13141 ggtggctgct ggagcgatta aaaaagcgat cgagcaggca gaagaaaagt caggtatccg
    13201 cattgaaaag gtaaatgttg gattacctgc caaccttctt cagattgaac cgactcaagg
    13261 gatgattcct gttacaacag attcacaaga aattacagac ttggatgttg agaatgttgt
    13321 aaaatcagca ttgacaaaga gtatgactcc ggaacgcgaa gtgatttcat ttattccaga
    13381 agagttcact gtcgatggat tccaaggcat taaggaccct cgtggaatga tgggaattcg
    13441 cttggaaatg cgtggtatgc tgtatacagg tccacgtacc attcttcaca atcttcgtaa
    13501 aacggtggag cgtgcaggtg tccaagttga aaatattgta atttctccgt tggcactaac
    13561 tcgctctgta ttgaatgaag gtgaacgtga gtttggtgcg actatcattg atcttggtgg
    13621 tggtcaaacg actgtggctg ttatgcgtgg ccaagagttg caatacacaa acatctacca
    13681 agaaggtgga gattatatca caaacgatat ttcaaaagta ttgacaactt caaagagtat
    13741 tgctgaaaac ttgaaataca actacggcat tgcctatccg caagatgcaa gtgataagga
    13801 aaaattcact gtcgaagtga ttggagaaaa cgctccagtt gaagtgaccg agcgctacct
    13861 ttcagaagtg attgcggcac gtctgcgtca gatttttgac cgtgtgaaac aagatttgga
    13921 gcgtacacgt gctctagatt taccaggagg cattgttatt gtaggtggtg gtgctatctt
    13981 accaggaatt acagaattgg cccaagaagt gtttggtgtt aacactaagc tttttgttcc
    14041 aaatcagatt ggtatccgca atccagcatt tgcaagtgtg attagctttg ttgagtatgt
    14101 tggtgagtta gaagatgtag aaaatattgc ccaacatgca gtaaatggtg aaactacctt
    14161 gagacataag ccagttgaaa ttcctgttgc tcgtcctcgg attagtcagc caattcaaag
    14221 agaaatcgtg aacgaattga acacagttga tcaggtagag gaacaagttg ctcctattta
    14281 ccatgatgag gaagaagaag ttcgtccagc tgctaaggga aacatcacag atcgcattcg
    14341 tggtttattt ggcagcatgt ttgaataaga gaaaagaaaa ggtgatttaa actatggcat
    14401 tttcatttga agcagcagct agtcatggtg ctgtcattaa agtaatcggt gtcggcggtg
    14461 gtggcgggaa cgcgattaac cgtatgattg aagaaggtgt tgctggggtt gagtttattg
    14521 cagcaaacac agatgtacaa gcgctaagta gctctaaagc tgaaacagtt atccaactag
    14581 gtcctaaatt gactcgtggt ttgggtgctg gaggtcaacc tgaggttggt cggaaagctg
    14641 ctgaggaaag cgaagaagca ttgactaatg tattaactgg tgcagatatg gtcttcatca
    14701 ctgctggtat gggcggtggc tcagggactg gtgcggctcc tgttattgca cgtattgcta
    14761 aaaacttggg tgcgttgact gtcgcagttg tgactcgtcc gtttggtttt gaaggaaata
    14821 aacgtggaaa ctttgcgatt gagggaattg aaggtcttcg tgaacaagta gatacacttt
    14881 taattatttc aaacaacaac cttcttgaaa ttgttgataa gaaaacaccg cttttagaag
    14941 cactaagcga agctgataat gttcttcgtc aaggtgttca aggaattaca gacttgatta
    15001 caaatcctgg tttgattaac ttggactttg cagatgtgaa gacagttatg gaaaacaaag
    15061 gaaacgcctt gatgggtatc ggtatcggta ctggtgagga ccgagtgatt gaggcggctc
    15121 gtaaagcgat ttattctcct cttcttgaaa caacaattga tggtgcagag gatgtcattg
    15181 tcaatgttac aggtggctat gacatgactc tgacagaagc agaagatgct tcagaaatcg
    15241 ttaatcaggc tgcgggtcaa ggtgttaaca tttggttggg tacatctatt gatgaaacaa
    15301 tgaaagatga gattcgtgtg accgttgtgg ctacgggtgt tcgtcaagat actgctgata
    15361 aaccagcccg ccatcgcatg gaagctgttt caccacgccc ttctcaacgc tttgatcatt
    15421 cagtagcatc agcaccaact cgtggagctg cacaacaaac tgaagcgccg aaagcttctg
    15481 ccttcggaga gtgggatttg cgtcgtgaaa acttgattcg tccaactgac acagaaccaa
    15541 catccactgt ttcagtcgaa aaatttacaa tggatcatga tgaggatgaa ttggatacac
    15601 caccattctt ccgtaatcgc taaactaaaa tgaattttca aaagaataag gatgctatct
    15661 tttcagtagt tgcaaaagct gctgagaagg caggtcgtcc aagtgaatca gtaaaggtaa
    15721 tagctgtcac caaatatgtt gacagctcta ttgctaatca gctagtgaat actggaatta
    15781 ctcatattgg agaaaatcga gtcgatcagt tcttagaaaa gtataatgcc ctagcagaca
    15841 aacaattgac atggcatttg atagggactt tacagagaag aaaggtcaag gacattattc
    15901 catttgttga ttatttccac gccctagact ctgtcaaact agctcaggaa atagataaac
    15961 gtgcgactaa aatagtcaag tgtttcctcc aagtcaacgt ttccggtgag gagagtaagc
    16021 atggctttgc agtatctgag attgatgcgg cacttgctga aattagttta ttggagaaca
    16081 ttgaacttgt tggattgatg acgatggcac cgattgatgc tacagatgat gaattagacg
    16141 agattttttc taaaatacag gaaatccaag aagaactagc tacaaggaac ttaccacgta
    16201 tgccctttac agagttaagt atgggaatga gtggggactt tgaacgcgcc attgctcacg
    16261 gagctacata tgtgagaatt ggctcagcat tctttgaata atggagagga ggaaaatatg
    16321 gcattaaaag atacatttaa gaacttattt aattactttg aagttgacga agtcaatgaa
    16381 gttgaagaac aggcagatgc ctattcaatg cctaatgatc gtcctaaaat gcgtgttgcc
    16441 aatactacag ttgcacctgt gcgcgaacag cagccaaaag tagagacacg tcgggaagca
    16501 cgttcagaaa gtcaacttca acgcctacat gaaaggcagc aagaattgat gactaataat
    16561 aacgaaaaag agattgtcaa aactacaatt gatattaagt ttccaaaacg ttatgaagat
    16621 gcaccagaga tggtaaatct tttattagac aatgcgagta ttctcattga ttttcaatat
    16681 atgtcggagc aacaagcaag acgttgccta gactacttgg atggtgctcg ttcagtatta
    16741 tctggaaatt taaaaaaggt ctcaaatacc atgtggctat taactccagt aaatgtcaca
    16801 gtcaatattg aagaattgcg caatgctaat gctggtacaa ctactggtgc ggctgattca
    16861 aattttgatt ttgatataaa acggtaacgc tatgcaaatt ttgatttttt tactattaaa
    16921 aattgtagag atttattctt atctcttatt ggcatacgct ttgctaagtt ggtttcctag
    16981 cctatacaca agctcgattg gtcgtttgat tcaatggctg gtagcaccga ttttaaaacc
    17041 ctttaagcga tttaatctac aatttatggg gttggattgg accgtaatgg cagccatgat
    17101 tgcgcttaat atgggaacac gcttcttggt tcaattattg ctactgttag catagttatg
    17161 aaaaatgaca aaaacttatt gcaacatttt tctagggaag aaagagagtt tgtagagaaa
    17221 ataatggata tgtgtcagca agttgaagat acgtattcat acagattaac tcacttttta
    17281 catcctagac aagatgaaat tgtatgcaaa attgctaact actaccagtt acaaacgttt
    17341 tctagtcgtg accttgtttc gacagaacat tcgcgggtta ttattgcccc gacttattat
    17401 gagttggaca tcaagcactt tgagttaacc gctcttcatt tgtcctatgc tagaaaattt
    17461 cacaccttat cccattcaca agttcttgga acttttctca atcaactagg gattaaaagg
    17521 gagtatttgg gtgatatttt gattgatgat gaacgattga ttgtctttat cgatcagaaa
    17581 tttgggcaga ttgccttaca atctatcact aaagttgctc gtgtccctgt taagggaaag
    17641 gaagaagaat ggacgactgt tcgactaccg attggacagg aatggcgttc aaaggatgtg
    17701 ttggtttcta gtatgagatt ggataaattg atttcggtcg catttaatct ttcaagggca
    17761 actgcaaaca agttgattga ggctggacat gtgaagttgg attatgttct gacagaacaa
    17821 accagtaaat tagttgaaat agggcaatta attagtgtta ggagatatgg tagagttcgc
    17881 ctaaatgaat ttttaggttt ttctaagcaa gggaagataa aattaaagtt agacattgtt
    17941 aaaacttagg aggaaagatg gcacttacag cattagaatt aaaagataaa acctttgcta
    18001 caaaatttag aggatacgac gcagatgaag ttgatgactt tttggacatt gtaacacgtg
    18061 attatgagga tttaattcgt aaaaatcatg atcaagagtt agaattgaaa aatttgcggg
    18121 aacgtttggc ttattttgat gagatgaaag aatcattgag taaatcagtt cttttagctc
    18181 aagatacagc tgagaaagta aaggttgcgg ccgaagatca agcggcaaat attattaaac
    18241 aagctgacta tgatgcggca acattgttac atgaagctaa agataaggca aatgaaattc
    18301 ttcgtaatgc gactgacaac gcgaaaaaag ttgtcattga aactgaagaa ttaaaaaacc
    18361 agacacgtat tttccatcag cgtctgaaat caacagtaga aagccaatta tcactggtta
    18421 attcatctga atgggaggaa atcctccgcc caacagcaag ttacattcaa acaagtgacg
    18481 aagcctttcg tgatgttctt cataaggctt tggatgaaga attacctgtt gaagaagaaa
    18541 gtttggatta tacacgtcaa ttgacaccag aagagattgc ggaattaact cgtcatgcag
    18601 cagcttttga gagtggagaa tctgtagtac tttcaataga agaataatag aaatcataag
    18661 caaagacaag gtcgttagaa atattttaag cgagtaggag atggtggaag ccctacaatc
    18721 tctttctatg gatcatcact ttgtttgatt tttcctgaaa atagtaagga gagacgtctg
    18781 ttcacgttac gaacatagag ggatagggga aactctatct aaactaaggt ggtaccacga
    18841 actttcgtcc ttatttggcg ggagtttttt tatttttatg atacgattag aattagtaaa
    18901 caaggataac tttgaagcag tgttacaagt tcaacttgca cctgaggacc aacgccgagt
    18961 agcatcagtt gaatattcat tagcccaagc ttggttatat aaagattcgg ggatgattct
    19021 accatatgct gtggtatctg gcagaaaagt tgttggtttt gcaatgttat ctatcgaaac
    19081 caaagataat agttattatt tatggcgctt gttgattgat aaggattttc aaaatcgtgg
    19141 atgcggaaag gaagtcgtcc agcaaatgat tggaaaggcg aaggcggatc cgctttgtca
    19201 taaaatttct ataaattatg tcattggtaa tcataaaatg cggtacatct tagaaaaatt
    19261 tggctttcag cctgtcggtt tagatggtca aaaaatgaaa atggaattaa ttataaaata
    19321 aaggagttat gatgaaacta aaagaaacac tcaatttagg taagacagca tttcctatgc
    19381 gtgcaggctt accaacacgt gagccagaat ggcaaaaggc ctgggatgaa gcaaacttgt
    19441 atgctcgtcg ccaagaactc aatgaaggca aacctgcctt ccacctccac gatggccctc
    19501 catacgctaa cggaaatatc cacgttggac atgctttaaa caagatttct aaagatatta
    19561 tcgttcgctc taagtcaatg tctggtttcc gtgctccatt tgtaccaggt tgggatacgc
    19621 acggccttcc tattgagcaa gtcttggcaa aacaaggtgt taaacgcaag gaaatggacc
    19681 tagttgaata tcttgaaatg tgtcgtgatt atgctcttag ccaagttgac aagcaacgtg
    19741 atgattttaa acgcttgggt gtctctgcag actgggaaaa tccatatatc actttgacaa
    19801 aagactacga agcagctcag atccgtgtct tcggtgctat ggcagacaag ggctatatct
    19861 accgcggtgc taagcctgtc tactggtcat ggtcatctga atctgccctt gcagaagctg
    19921 aaatcgaata ccatgacatt gactcaacat ctctgtatta tgccaacaag gtcaaagatg
    19981 gcaaggggct cttggataca gacagctaca tcgttgtctg gacaaccact ccatttacag
    20041 taacggcttc tcgcggtttg acaatgggag cagacattga ctatgtcttg gttcaacctg
    20101 ctggctctga ccgtaagtat gtcttggcag aagccttggt tgatagcttg gcagccaaat
    20161 tcggttggga atccttcgaa gtgatttcaa aacacaaggg tgctgaattt gaatatatcg
    20221 tgaccgaaca cccatgggat actgaagttg atgaattggt tatcttgggt gaccatgtca
    20281 caacggactc gggtactggt atcgtccata cggctccagg ttttggtgag gatgactaca
    20341 atgtcggtgt caaatatgac ttggaggttg cggtaactgt caacgaacgt ggcttgatga
    20401 atgaagctgc gggtcctgat tttgaagggc aattctatga caaggttgta ccaacggtta
    20461 aggaaaaatt aggcgactta ctccttgcta gtgaagtgat caatcactcc tacccatttg
    20521 actggagaac caagaagcca atcatctggc gtgcagtgcc acagtggttt gcctctgttt
    20581 ctaaattccg tcaggaaatc ttggaccaaa tcgaagcaac gacctttatt cctgcttggg
    20641 gtaaaacacg tctttacaac atgatccgtg accgtggtga ctgggtcatc tctcgtcagc
    20701 gtgcttgggg tgtgccactt ccaatcttct atgcagaaga tggaacagcg attatgacca
    20761 aggaagtgac agaccacgtt gctgcccttt ttgaagagca tggttctatc atctggtgga
    20821 aatcagaagc aaaagatctc ttgccagctg gtttcacaca tccaggttca ccaaatggcg
    20881 aatttaccaa agaaacagac attatggacg tatggtttga ctctggttca tcttggaatg
    20941 gtgtcatgaa cgcccgtgaa aaccttgcct acccagcaga cctctaccta gaaggttctg
    21001 accaataccg tggttggttt aactcatcct tgattacctc tgttgctgta aatggtcatg
    21061 cgccatacaa ggctgtcttg tcacagggct ttgtattgga cggtaaaggt atgaagatgt
    21121 ctaaatccct aggaaatact atccttccaa gtgatgttga gaagcagttt ggtgcagaaa
    21181 tcttgcgtct gtgggtgaca tccgttgata cttcaaacga tgtccgtgtg tctatggata
    21241 ttcttggaca ggtttctgaa acctaccgta aaatccgtaa tacccttcgc ttcttaattg
    21301 ccaacacttc tgatttcaat cctgcaagtg atgcggtggc atacgaagag cttcgttctg
    21361 ttgaccagta tctcttggtg aaattcaata aattggtagc tcaaatccgt gaagcctacg
    21421 acaattatga tttcatgggc atctataaat ctgtagtaaa ctttgtaacc cttgatttgt
    21481 cagccttcta cctcgatttt gccaaagatg tcgtctacat tgatggtgct aaatcacttt
    21541 ctcgtcgtca gatgcaaact gtcttctatg acatcttggt gaaaattacc aagctcttga
    21601 cgccaatctt accacatacg gcagaagaaa tctggtctta cttggaacac gaagcagaag
    21661 aatttgttca gttggcggaa atgccagagg tggaaacctt tgctaatgaa gcgcagcttt
    21721 tagctgattg ggaaaacttt atggtcttcc gtactcaggc acaaaaagcc ttggaagaag
    21781 cacgcaatgc caaagtgatt ggtaagtcac ttgaagctca tttgactgcc tatgtctcag
    21841 ctgaaaccaa gtccttcttg gagagcttga atgcagacct tgctcagttg ctcatcgttt
    21901 caaacttgac agtaaccacg gaagcagctc ctgaaagtgc tgttttggta gaagaagttg
    21961 cctttactgt agagcgtgca agcggtgaag tatgtgaccg ttgccgccgt attgatacta
    22021 gtgttgcaga gcgcagctat ggcgcaacaa tctgtgacca ctgtgcaagc gtagtggaag
    22081 aaaactttgc tgaagcagta gctcaaggtt ttgaagccta agcaatcaca atacatttgt
    22141 taaagtctat ctagcgat
//
LOCUS       DASFSW010000017        21552 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.017, whole genome shotgun sequence.
ACCESSION   DASFSW010000017 DASFSW010000000
VERSION     DASFSW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21552
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.017"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(148..939)
                     /locus_tag="U1099_000533"
     CDS             complement(148..939)
                     /locus_tag="U1099_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM4866877.1"
                     /translation="MKKFSCKSVLALAGITVALSTISRVQATSHVVMAGDSMFSIAEQ
                     YGVDPYQLAEVNAMEIDGLITPGQRLDLPAGSNEGVSVAGQTDYVVQDGDSFYGIADA
                     FSLDINKLLEQNQLTLDANIHPGQILKLTETIWNQTVDTAADSYYLPGYEYEPGINYP
                     VGQCTWGVQKVAPWAGDWWGDAASWGANAARDGFRTGTTPEVGAIISWNDGALGHVAY
                     VTAVNEDGQIQVLEANYRGQQWVDNFRGWFNPTDTIGEITYIYNN"
     gene            complement(1021..1572)
                     /locus_tag="U1099_000534"
     CDS             complement(1021..1572)
                     /locus_tag="U1099_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEM4866878.1"
                     /translation="MRYVLLLRGINVGGRNKIVMAELRQAVADLGYDKVETYINSGNL
                     FFDSTKNRGDIVAEFQTFFTERYPFVEQFALLDTQDYQAEIDQLPAWWQEELARKDVL
                     FFTEGLDKQAMVDYIQSLHLGDEVLHFSDYAIYWGKYTEGSYLQTAYHKQLAKSPFYK
                     QVTIRNGKTFSVISNYLENEIKR"
     gene            complement(1569..2510)
                     /locus_tag="U1099_000535"
     CDS             complement(1569..2510)
                     /locus_tag="U1099_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEM4866879.1"
                     /translation="MRRVILDKVSEQIEQGVYPGASLAFFSEGQWQEYYLGTQDGHTP
                     VEAGLTYDLASVSKVVGVGTLATFLVNSGALELDRTLQSYYPAFADSEVTIRQLLTHT
                     SGIDPFIPNRDSLDADQLKEAILKIAVTDKKAFLYTDINFLLLGFLLEELGRDSLDQL
                     ISRDVLEPFGMSQTSFGPVSQAVPTVRGVKAGVVHDPKARVLGLHAGSAGLFSTVKDL
                     EIFLEHYLMADFAANLTQDYGFDVKRKRSLAWDKKGDWLSHTGYTGTFIMYNRPLQKA
                     AIFLSNRTFDKDERAQWKLDRNQVMALIRQVLEEEGQ"
     gene            complement(2507..3241)
                     /locus_tag="U1099_000536"
     CDS             complement(2507..3241)
                     /locus_tag="U1099_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEM4866880.1"
                     /translation="MLRKNEAIVPVLRINNRAINQGFLEKNLGMKTKLEDGPFADFGD
                     RTNPETKLVLTESPGNRTRAVEGLKKLNKIVIKVDNASEIEALLAQGSQYSKLYQGKN
                     GYGFEAVSPEGDTFLLHAEASVDDLKAILPPVPFKVQDDFSGLTAFTVESVWINTPQA
                     SINQDYYEAILPQQAFLRFIGAEGEDLLTPAEQVWDLEGLRFPVEQDFDWAHLESRLE
                     GPFFKDKKASFIQTVDPSGIELWFEK"
     gene            3423..5690
                     /locus_tag="U1099_000537"
     CDS             3423..5690
                     /locus_tag="U1099_000537"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEM4866881.1"
                     /translation="MRFNQFSFIKKETSVYLQELDALGFQLIPNVSSKTNLETFVRKC
                     HFLTANTDFALSNMIADWDTDLLTFFQSDRELTDLIFYQVAFQLLGFVPGVDYTDVMD
                     FVEKSNFPVIYGDMIDNLYQLLATRTKSGNTLIDQLVSDDLIPEDNRYHFFNGKSLAT
                     FSTKNLIREVVYVETPVDTAGTGQTDIVKLSILRPHFDGKIPAVITNSPYHQGVNDVA
                     SDKALHKMEGELAEKQVGTIQVKQASITKLDLDQRELPVSPSTEKLGHITSYSLNDYF
                     LARGFASLHVSGVGTLGSTGYMTSGDYQQVEGYKAVIDWLNGRTKAYTDHTRSIEVKA
                     DWANGKVATTGLSYLGTMSNALATTGVDGLEVVIAEAGISSWYDYYRENGLVTSPGGY
                     PGEDLDSLTALTYSKSLQAGDFLRSKEAYEKGLAAERAALDRTSGDYNQYWQDRNYLL
                     QADKVKCEVVFTHGSQDWNVKPIHVWNMFHALPSHVKKHLFFHNGAHVYMNNWQSIDF
                     RESMNALLSQKLLGYENNYQLPTVIWQDNSGEQTWTTLDTFGGENEAVLPLGTGSHTI
                     ANQYAQEDFDRYGKSYPAFHQDLYAGKGNQVSIDLPVTEDLLLNGQVTLKLRVASSVA
                     KGLLSAQLLDKGNKKRLAPIPAPKARLSLDNGRYHAQENLLELPYVDMPQRLVTKGFM
                     NLQNRTDLMTVEEVVPGQWMNLTWKLQPTIYQLKKGDVLELILYTTDFECTVRDNSQW
                     QIHLDLSQSQLILPH"
     gene            complement(5970..8378)
                     /locus_tag="U1099_000538"
     CDS             complement(5970..8378)
                     /locus_tag="U1099_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEM4866882.1"
                     /translation="METANKKFRYSIRKFKVGVGSVLIATCLLGAGVSTPTAFATTET
                     STATQVEAAQPTSETEPTVQITGTVSTENTNSESKVAPEVKEEAPVAEKAETATNSAE
                     QNVVSEPEKSEEPTPAPAALTSEAKEEVSENTEVKSAPLPQFQDILKELERDLEELKV
                     FRDYTGSDYSFQKGLWTYLLEDGQKLMSYVQTLEKDSPKIKENYDKLLDFKNHIRMER
                     LTRQVVPYLEKYSGNEFIRSEYREHLEQSEENGSYASQEGKVFEAYVDSAEQAFDRIV
                     NRVNELESKNLQKKVPDTAPKVAEKPKLDFKVQTRTTYQFLDFKTKEQEDPNLPLGEK
                     VERQKGERGEESETYQDVIVGDKIVASTLISKKRKDPVDHLIAKGTLVEKHPDTAPTA
                     PEKPKLDFTVKERVEREILDYMVEEQDDASLPLGQTKVLREGQKGELVKKYQDVIVEG
                     KVIESKLLSEDRKTPVNRLIAKNILVEKHPDTAPTAPEKPKLDFTVKERVEREILDYM
                     VEEQDDASLPLGQTKVLREGQKGELVKKYQDVIVEGNVIASNLLSEDRKAPVNRLIAK
                     GTLVEKTPDAPAPKAEEEVPASTPMPETPMDKPKTDKVESDKQMPEAKQPEMEQPKAE
                     DMPKAEQPKAEDSAPKTAVPEVAPKTAEKPKLDFTTKERKVEEALPIKEEIRYDASLA
                     LGKSYLLQEGKAGKKVSVYQDVIVDGKIVATNLLSETVVEGQNRILVKGSLEMKKEEV
                     KSTPSVQSNLTLSHKGAPSANKATLPATGEQRNNLALVGLGLAGISLAVVATAINKKS
                     KDQI"
     gene            complement(8508..9677)
                     /locus_tag="U1099_000539"
     CDS             complement(8508..9677)
                     /locus_tag="U1099_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM4866883.1"
                     /translation="MFRLAKEELEKLGAMITATEIYQQPGLWKEAHQLYFDQLEKIES
                     FLKSIKEKHDFVHVVFTGAGTSDFVGQSIANYLNQVNDLKHIRFSAIGTVELVSRPHD
                     YLQADLPTILVSFARSGNSPESVAAVEVAKQLVDKLYQVTITCAPEGKLALAAEGDVE
                     NLLLLQPAGSNDKGFAMTGSYSCMALTALLVFSPASQEEKAAWVEIIARLGQDVLDRE
                     DYVQDLINLDFERVIYLGSGGFYGLAHEAQLKILELTAGKIATMYESPLGFRHGPKSF
                     INEKTLIFLFASNDAYTRQYDVDLLNEVHGDGIAERIVALSADKLVGTEAANFVLAEG
                     GADLPDVFLTFPYILFAQTVSIMAAIKCNNLPDTPSPTGTVNRVVQGVIIHPLEK"
     gene            9956..10255
                     /locus_tag="U1099_000540"
     CDS             9956..10255
                     /locus_tag="U1099_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEM4866884.1"
                     /translation="MMNMQNMMRQAQKLQKQMEKSQAELAAAQFTGSSVQDLVTATFT
                     GDKKLVSIDFKADVVDADDIETLQEMTVQAVNAALTKVDEATQKKLGAFAGKLPF"
     gene            complement(10295..12055)
                     /locus_tag="U1099_000541"
     CDS             complement(10295..12055)
                     /locus_tag="U1099_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEM4866885.1"
                     /translation="MKKIQREFQKIYYNLDKILLLFFTLFTFMEFVWIPLNSWISEGL
                     LAMTGHAYLSPTNLLSVFAENLLVTGLFILLFFVNIAIAYLELALLFTGVWQLLDEKV
                     KHLPDYLRDVRDSMVAIIRHSSLPKVLFLLFYSVILLPFLRRVLNIYYFNKIVVPQFI
                     VDYLSNTVWLGILILIFLLLFFWLAARFMYALPNIYFEHRGVKESIAYSWEKTRGRKQ
                     IGSLFRLIWLITFPVLLFTGVGGLFYLVQLLAEHFVPSASYWLAVVCYALLKLAYYGV
                     VALFMMGFVSLLTGKQLPICRRKRLRHHLRLAILLVSGLVFGTQGALALYFPFDTLPV
                     TISHRGVDDGNAVQNSIEALEKTSQLKPDYIEMDVQETKDGQFIVMHDTDLLALTGNP
                     GGTHDYTLAELTGMTASENGMVAPVPSFDEYLEKADELGQKLLVEIKVTKADSPKLTQ
                     NFLKKYGQRLIAKGHQMQSLDYRTIIEIKQYSEKLVSFFILPFNSIYPTTVADGYTME
                     YTSLDQNFMTKSWIRGKSVYAWTPNEEESMTKMLLLQVDGIITDNMTDLQSLMEEMKE
                     NRRYPDLFFQQFQSLIYNFE"
     gene            complement(12246..13172)
                     /locus_tag="U1099_000542"
     CDS             complement(12246..13172)
                     /locus_tag="U1099_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEM4866886.1"
                     /translation="MRQTKEMRLVYSDFLAQVGQEDTTIAAIEADLSSSMATNKLSSV
                     LGGRYVNVGIMEQEMVGVAAGLSLLGYKPYIHTFGPFASRRVYDQVFISLAYAQLNAT
                     VIGSDAGVSAEMNGGTHMPFEELGLMRLIPKARVYEVSDDVQFQAVLKETIKVDGLKY
                     IRTIRKATTAIYQGGEDFSKGYCVLRQGEDMTLLASGIMVEQALKAADQLEAQGVSVQ
                     VIDLFRIKPIHEDIPALLSGRPVLTVENHNRIGGLGSSICELMATDLTTPVHRIGIEE
                     SFGQVGQQAYLMDVYGLTAEYIVKQAQLMLQQ"
     gene            complement(13169..14017)
                     /locus_tag="U1099_000543"
     CDS             complement(13169..14017)
                     /locus_tag="U1099_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEM4866887.1"
                     /translation="MTSGTADLKRFAKEIRYHTLATLNQLGFGHYGGSLSIVEVLAAL
                     YGEILDIRVQNFSSKERDHFILSKGHAGPALYSTLYLKGFFDRDFLLSLNQNGTHLPS
                     HPDRNLTPGVDMTTGSLGQGMSVATGVAYGKKIEQSPYYTYTLVGDGELNEGQCWEAA
                     QFAAHHELSKMILFVDDNKKQLDGRTHDICQTFDFVEKFQAFGWESVRVNGADIDAII
                     NAILTMKSSKSPQPKCIVLDTTKGQGVTFLEELESNHHLRLSGQLREDLEQVTLVLAR
                     ELEVME"
     gene            complement(14019..15365)
                     /locus_tag="U1099_000544"
     CDS             complement(14019..15365)
                     /locus_tag="U1099_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEM4866888.1"
                     /translation="MVLDFLIDIAKTPAILVALIAILGLALQKKPAADLIKGGLKTFV
                     GFIVVGGGANIIVGSLEPFGQMFEQAFNLKGVVPNNEAIVAMALDKYGTATSLIMLLG
                     MVFNIAIARFTRFKYIFLTGHHTLYMACMIAVIMSVAGFTSVGLIVMGGLALGLIMTL
                     SPAFVQKYMIQLTGNDHVALGHFSALGYWLSGVVGGLVGDKSKSTEDINFPKGLAFLR
                     DSTVSIAISMSLIYMIVALFAGGDYIKENLSGGTNSMIYALTLGGTFAAGVFVILSGV
                     RLILAEIVPAFKGISEKLVPNSKPALDCPVVYPYAPNAVLIGFISSFAGGIVSMIILA
                     LTGGVVILPGVVPHFFCGATAGVMGNASGGVRGAVCGAFMQGVLISFLPVFLLPVLGD
                     LGFAGSTFSDADFGLSGIFLGVLAKNGGILTVSVGIFAVLALMLGLSLVKKEQKEG"
     gene            complement(15419..15703)
                     /locus_tag="U1099_000545"
     CDS             complement(15419..15703)
                     /locus_tag="U1099_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM4866889.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILRDLGVSDVHVEHYDLGGA
                     NPSEADVWIVGRDLADSATHLGDVRILNSIIDMNELRELVTAICQEKGLV"
     gene            complement(15705..17747)
                     /locus_tag="U1099_000546"
     CDS             complement(15705..17747)
                     /locus_tag="U1099_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM4866890.1"
                     /translation="MVDNRTMAILMELFATRKESLYELSIQTGIEKEQLHSNLELVNQ
                     LLQEHSFPQIQYKDSEFVISTELYNQKESVFSLFRNRQIYLSQEERQLLIYLYTFIRK
                     EFVSNVHYQELLSVSRNTTLTDIKNVKELCLDFQVRLEYTRAKGYHLIGREEDKHRLA
                     LYALSNCLQSSIGVWALDYILKSWGEENPIEELKIVSQQACNYYKVSALEERLDEYLY
                     FLIFLFIRQARVGNRINWNFEGKGGFVKDFLQRLWKLLRLKSTSTLELNAATQEYLSH
                     LLQGCLEGESGEKDDLFYHLTVEIVEEMERLSLIAFEHRSEMIEGLQRHLIPAYYRLT
                     SRLVNVNSYTETIKEEHADLFYLVKKALRPLEEHLGFTIPDSEVSYFVIHFGGYIEAG
                     QQRSYRYRALVVCPNGVSSSLIVKENLRQLFPNIYFADTHSLQDWKMLDMTDYDMVFG
                     TIKLNIELPFFLVSQLMTSNHKKELFHLVNQHFPNAAYFPIEIEQLLSLVGKHATIHQ
                     EQALKYELVQFLNQRSHEQRGRSPMLEELITKETFQWSEEVLDWQKAVALVARPLVDK
                     GAIETRYIDAMIAKVEEFGPFIDLGKGIAIPHARPEDGVNEVGMSMLVLDKPVYLLDD
                     PSHEIRLFICIAAVDNQTHLRALSHLTKILRAEENIQQLVGAKEFADVQHLLKEEQ"
     gene            complement(18068..18562)
                     /locus_tag="U1099_000547"
     CDS             complement(18068..18562)
                     /locus_tag="U1099_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEM4866891.1"
                     /translation="MGIEKTVSELAEILGVSRQAMNNRVKSLPEEFVDKNDKGVTVVN
                     RAGLIKLEEIYKTTIFEDEPISDEVKHRELMEILVDEKNAEIIRLYSQLKAKDKQLAE
                     KDEQLKVKDVQIAEKDKQLDQQQQLTLKAMADKEVLKLELDEVKAQAEEVQAKGFFAR
                     LFGK"
     gene            complement(18732..20132)
                     /locus_tag="U1099_000548"
     CDS             complement(18732..20132)
                     /locus_tag="U1099_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM4866892.1"
                     /translation="MVTEQREYRFPEGFLWGSSTSGPQSEGSVAGDGKGWNNWDYWYS
                     QEPELFHQQIGLEQTSTFYEHFQSDLDLLVETGHSIFRTSIQWSRLIPEGIGEVNPQA
                     VEFYRAVFAGIRERGIKLLVNLYHFDLPYVLQEKGGWENKEIVWAYEAYARQCFSLFG
                     DLVDHWITFNEPIVPVECGYLGSYHYPCKVDAKAAVVVAYHTQLASSLAVRACHQLHP
                     YHKISIVLNLTPAYPRSQSPEDLQAARIAELFQTRSFLDPSVLGQYPRELVDILEKHG
                     LRPETTEEELTIIKENRVDFLGVNYYQPLRVQAPSEDGWNGEATFLSQYFQPYDKPDK
                     KINPHRGWEIYEEGLYDIAKNIQENYGNIEWLVTENGMGVEGEDVFEENGMIQDDYQI
                     HFMEDHLIQLHRGIDEGANCKGYMVWTFIDCWSWLNTYKNRYGLVSLDLQTQKRTVKK
                     SGYWFKQVSAKNGFIR"
     gene            20370..21545
                     /locus_tag="U1099_000549"
     CDS             20370..21545
                     /locus_tag="U1099_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM4866893.1"
                     /translation="MNTVDTIIIGAGPAGMMAAISSSFYGKKTLLLEKNKRLGKKLSG
                     TGGGRCNVTNNGTLEDLLAGIPGNGRFLYSVFSQFDNHDIMNFFQENGVKLKVEDHGR
                     VFPTTDRSQTIIKCLEMKMLENGVNIRTGTEVVSVRKIDDLFHVKTSEETFTAPQLIV
                     TTGGKTYPSTGSTGFGHDIARHFKLEVTEIEAAESPVLTDFPHKKLQGISLDDVTLTY
                     GKHVITHDLLFTHFGLSGPAALRLSSFVKGGETAFLDALPTHSDQDLFEHLEANREKS
                     VKNALRELMPDRLADFFAENYDCKVKQVSQKDLTDLVSLLKALPIKITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLHFAGEVLDINAHTGGFNITYCLATGWVAGSSYSS"
BASE COUNT         6163 a         5090 c         4099 g         6200 t
ORIGIN      
        1 tttttactat gtcgaaaaat ctactgagtg ttgcggaagg atagtaaatc ttcggaagga
       61 tgcaccgcag attttcggaa ggatacagaa ccagttcata aaaaaatccc tttcgaaatt
      121 ggattcgaaa agggatgatt atttatccta gttattatag atataggtaa tttctccgat
      181 ggtatccgtc ggattaaacc agccgcggaa attatccacc cactgttggc cacgatagtt
      241 ggcttcgagg acttgaattt gtccgtcctc gttaacagct gtcacatagg caacgtgtcc
      301 cagagcacca tcattccaag aaataatggc tccaacttca ggtgttgttc ctgtacggaa
      361 accatcacgc gccgcattgg ctccccaaga agccgcatcg ccccaccagt cacctgccca
      421 aggggcaact ttctgtactc cccatgtaca ttgaccaaca gggtagttaa tgcctggttc
      481 atactcatag cctggtaaat agtaactgtc agcagccgta tcaaccgtct gattccaaat
      541 tgtttctgtt aacttgagaa tttgacctgg atgaatattg gcatctaatg tcaactgatt
      601 ttgttccagc aatttattga tgtccaatga aaaagcatct gcaatcccgt aaaacgaatc
      661 cccatcttga acaacatagt ctgtttgacc cgcaactgag actccttcat tagaaccagc
      721 tggaagatcc aaacgttgac ctggtgtaat cagtccgtct atttccatcg cattcacttc
      781 agccaattgg tagggatcaa ccccatattg ctccgcaata gagaacatgg aatcccctgc
      841 cattactaca tgactagtag cttgaacacg agatatggtt gataacgcaa ccgtaatccc
      901 tgcaagagct agaacagatt tgcaactaaa ctttttcata tgacacctcc tcatcatgat
      961 ttcattttac caagattttg acgggaaaag atgtcgaaag cattacaaat aaatgacgat
     1021 ttatcgtttt atttcattct ctaaatagtt tgatataact gagaaagtct tcccattccg
     1081 aatggtcact tgtttgtaaa aaggtgactt agccaactgc ttgtggtagg ctgtttgtag
     1141 atagctacct tcagtgtatt tgccccaata aatagcatag tcggagaaat gtagaacttc
     1201 atcacctaga tgcaaggact gaatatagtc caccatagcc tgcttatcca atccttcggt
     1261 gaaaaagaga acatcctttc tcgccaattc ttcttgccac caagctggaa gctgatcgat
     1321 ttctgcttga taatcttggg tgtctagtag agcaaattgc tccacaaagg ggtatctttc
     1381 tgtgaaaaaa gtttgaaatt cagcaacaat gtcccctcta ttcttggttg aatcaaagaa
     1441 gagattaccg ctattaatgt aggtttcaac cttgtcatag cccaaatctg ccaccgcttg
     1501 acgcaattca gccatgacga ttttgttacg accaccgaca ttgatccctc tcagcagaag
     1561 aacatatctc attgcccctc ttcctccaag acctgacgaa tcaaggccat aacctggttt
     1621 ctatccaact tccactgggc ccgctcatcc ttgtcaaaag tacgattcga gagaaaaatc
     1681 gcagctttct gcaagggacg gttatacatg ataaaggtcc ccgtatagcc tgtgtgcgac
     1741 aaccagtccc ccttcttatc ccaagccaga gaccgcttac gcttaacatc aaaaccgtaa
     1801 tcctgcgtta aattcgccgc aaagtcagcc atcagatagt gttccaaaaa gatttccaaa
     1861 tctttgacgg tcgaaaagag acctgcactt cccgcgtgga ggcccaagac acgcgccttg
     1921 ggatcatgca ccacacctgc tttgacgcca cgaaccgtcg gcaccgcctg actgactggt
     1981 ccaaaggagg tttgggacat accaaagggc tctagaacgt ctcgactaat cagctggtct
     2041 agactgtctc tacccaattc ctccaacaaa aagcccagta agagaaaatt aatatccgtg
     2101 tagaggaaag ctttcttatc cgttacagca atcttcaaaa tcgcttcctt gagctggtcg
     2161 gcatccagac tatccctatt tggaataaag ggatcaatac cactagtatg ggtcaataat
     2221 tgccgaatgg tgacctcact atccgcaaaa gctggataat aagactgcaa ggtcctatct
     2281 agctccaaag ctccgctatt aaccagaaaa gtcgcaagag tccccacacc aaccaccttg
     2341 gaaacagacg ccaaatcata ggtcaaaccc gcctcaactg gcgtatgccc atcctgagtc
     2401 cctagatagt actcctgcca ctgaccctca gaaaaaaaag ccagactggc accaggataa
     2461 actccttgct caatctgctc tgaaaccttg tctaaaatta ctcgtctcat ttttcgaacc
     2521 acaattcaat accacttggg tcaactgttt ggataaagct tgctttttta tctttaaaga
     2581 atggtccttc caaccggctt tccaaatgtg cccaatcaaa gtcctgctcg acgggaaaac
     2641 gcaatccttc taaatcccaa acttgttctg caggagttaa taaatcctca ccttctgctc
     2701 caataaaacg caaaaaggct tgttgaggta ggatagcctc ataatagtct tggttaatac
     2761 tagcttgtgg ggtattaatc cagacagact ctactgtaaa agcagtcaat ccagaaaagt
     2821 catcctgcac cttaaatgga acaggtggaa ggattgcttt taggtcatca acagacgctt
     2881 ctgcgtgtaa aaggaaggtg tctccttctg gcgacacagc ttcaaaacca tagccatttt
     2941 ttccttggta gagcttacta tactgtgacc cttgcgccag taaagcttca atttcagagg
     3001 cattatctac ttttatcaca attttattca attttttaag cccttcgaca gcacgggtgc
     3061 gattaccagg agattctgtc aaaacgagct tcgtttctgg atttgttcta tctccaaaat
     3121 cagcaaaagg accatcttct agcttggttt tcattcctag atttttttct aaaaatcctt
     3181 gattaatagc cctattatta atacgtaaaa cagggactat agcttcattt tttctcaaca
     3241 cgatacattt cactcctcag tcattctaca atattgtata aaaaatttcg tcattttaca
     3301 actaattata tcgtcgtttt atacattaat atctttatat atactgataa tttttagaca
     3361 aacattgact tatccgatat aatagctatt atagactatt atcctaaaaa agaggagaat
     3421 ttatgcgctt taatcaattt tctttcataa aaaaagagac ctccgtctac ttgcaagaac
     3481 ttgatgctct aggatttcaa ctgattccta atgtaagtag taagacaaat ttagaaacct
     3541 ttgtcagaaa gtgccatttc ctgacagcaa acaccgactt tgccctgtca aacatgatcg
     3601 cagattggga cacagacctg ctgaccttct tccagtctga ccgagagctg acagacctga
     3661 ttttctatca agtggccttt cagttgctag gcttcgtgcc tggtgtagat tatacagatg
     3721 tcatggattt cgtggagaaa agcaatttcc ctgtcatcta tggagatatg attgacaacc
     3781 tctatcaact gctggctact cgtaccaagt ctggaaacac cttgattgac cagctggtca
     3841 gcgatgacct aatcccagaa gacaatcgct accacttctt caacggcaaa tctttggcga
     3901 ccttttcaac caaaaacctt attcgtgagg tggtctatgt cgaaacgcct gtcgatacag
     3961 ctgggactgg gcagacagac attgttaagc tgtccattct ccgtccgcac tttgacggaa
     4021 aaattcctgc ggtgattaca aacagtcctt atcatcaggg agttaacgat gtagccagcg
     4081 acaaggccct gcataagatg gaaggggaac tggctgaaaa acaagttggt accattcagg
     4141 tcaagcaggc cagcatcacc aagttagact tggaccagcg cgagctccct gtcagccctt
     4201 ccaccgaaaa actgggccac atcacttcat actccctcaa tgactatttt cttgctcgtg
     4261 gtttcgccag cctccatgtg tctggtgtcg gtacgctggg ctcgacgggc tacatgacat
     4321 ctggcgacta ccaacaggtg gagggctata aagcggtgat tgactggctg aacggtcgca
     4381 ccaaggccta cacagaccac acccgctcga ttgaggtcaa ggccgattgg gccaatggta
     4441 aggtggcgac gacgggactg tcttacctcg gcaccatgtc caatgccttg gcaacaactg
     4501 gcgtggacgg tttggaagtt gtcatcgcag aagcagggat ttcctcttgg tatgactact
     4561 accgtgaaaa cgggctggtg accagccctg gcggctatcc gggcgaggac ctggacagct
     4621 tgactgccct gacctactcg aaaagtctgc aagcagggga tttcctccgc agcaaagaag
     4681 cctacgaaaa aggcctggct gccgaacgag ctgccctaga ccgcaccagc ggtgactaca
     4741 accaatactg gcaggaccgc aactacctgc tccaggctga caaggtcaag tgcgaggtcg
     4801 tatttaccca tggctcacag gactggaatg tcaagccaat ccacgtctgg aatatgttcc
     4861 atgccctgcc aagtcacgtc aaaaagcacc tcttcttcca caacggtgcc cacgtttata
     4921 tgaacaactg gcaatccatc gacttccgcg agtccatgaa tgccttactc agccagaaat
     4981 tgctaggcta cgagaataac tatcaactac caactgtcat ctggcaggac aacagcggtg
     5041 agcaaacttg gacgaccttg gacacctttg gcggtgaaaa cgaggctgtc ttgccactag
     5101 gtactggaag ccataccatt gccaatcagt atgcccaaga agattttgac cgctacggca
     5161 aatcctaccc tgccttccac caagacctct atgcaggcaa gggcaaccaa gtcagcattg
     5221 acctgcctgt aacagaggac ctcttgctta acgggcaggt cacgctgaaa ctccgtgttg
     5281 cttctagtgt agctaaaggt ctcttatctg ctcagctatt ggacaaggga aataaaaaac
     5341 gcctagcccc aatccctgcg cctaaagcaa gattgagtct agacaacggt cgttaccatg
     5401 cccaagaaaa tctgctggag ttgccttatg tggatatgcc acaacgcttg gtaaccaagg
     5461 gctttatgaa cctgcaaaac cgcaccgacc tgatgactgt cgaggaagtg gtgcctggac
     5521 agtggatgaa tctgacctgg aaactgcagc cgaccattta tcagctgaaa aaaggcgatg
     5581 tcttggagtt gattctctat acaaccgact ttgagtgtac tgttcgggat aattctcaat
     5641 ggcaaatcca ccttgattta agtcaatcac agttaatttt accgcactaa tactcaatga
     5701 aaatcaaaat cagactagtt tcaaaggttt gggaaacctt tgaaggttgg agatagagtg
     5761 aacgaagttc gtttctccaa gagcagatat acccctgcta ctttcttatt ttatggtagc
     5821 aggctgtcaa gctccactgg agcttgacac tcatcaaatc aagtcaacaa cgtctgattt
     5881 tgagtttcga agagtataat attgattttc agttctaaaa agaggctggg cgtttccgcc
     5941 ctgcctcttt attgatattt cataatggtt tagatttgat ctttagattt tttgtttata
     6001 gctgtagcga ctactgccaa gctaattcca gctaaaccaa ggcctactaa ggctaggtta
     6061 ttgcgttgtt cacctgttgc aggtagggtt gctttgtttg cagaaggtgc gcccttatga
     6121 ctcagtgtca gatttgattg tactgaagga gttgatttta cttcttcttt cttcatttcc
     6181 aagctacctt ttacaagaat acgattttga ccttcaacaa cagtctctga caataggttg
     6241 gttgcaacaa ttttaccatc aactatgaca tcttgataaa cagatacttt tttacctgct
     6301 tttccttctt gaagaaggta tgatttgcca agcgctaggc ttgcatcata tctgatttct
     6361 tctttgatag ggagagcttc ttctaccttg cgttcttttg ttgtgaagtc tagctttggt
     6421 ttttccgctg tttttggtgc gacttctgga actgctgtct taggtgcaga gtcttccgct
     6481 tttggttgct cagcctttgg catgtcttct gctttcggtt gctccatttc tggttgctta
     6541 gcctctggca tctgtttgtc agactctact ttatcagttt tcggtttgtc cattggagtt
     6601 tctggcattg gagttgaggc tggaacttct tcctctgctt tcggcgctgg agcatctggc
     6661 gttttttcca ctaaagtgcc ttttgcaatc agacggttga ctggggcttt acggtcttct
     6721 gaaagaagat tacttgctat gacattccct tcaacaatca catcttggta tttcttaacc
     6781 aattctcctt tctgaccttc acgaagtacc tttgtttgac cgagtggcaa actagcatca
     6841 tcttgttctt caaccatata atctaaaatt tctcgctcta ctcgttcttt aacggtaaag
     6901 tctaatttag gtttttcagg agctgtcggt gctgtatcag gatgtttctc tactaaaatg
     6961 ttttttgcaa tcagacggtt aactggggtc ttacggtctt ctgaaagaag tttactttca
     7021 atgactttac cttcaacaat cacatcttgg tatttcttaa ccaattcacc tttttgacct
     7081 tcgcgaagaa cctttgtctg accaagtggc aaactagcat catcttgttc ttcaaccata
     7141 taatccaaaa tttctcgctc tactcgttct ttaacagtaa agtccaattt tggtttttca
     7201 ggagctgtcg gtgctgtatc gggatgtttc tctaccaaag tacctttagc aatcagatga
     7261 tcaactggat ctttacgttt tttggaaatg agggtacttg caacaatttt atccccaaca
     7321 atcacatctt ggtaagtctc agactcttcg cctctctcac ctttttgtcg ttccactttc
     7381 tcaccaagtg gcaagtttgg atcttcttgc tctttcgttt tgaaatctaa aaattgatat
     7441 gttgttcgtg tttgaacttt aaaatctagt tttggttttt cggctacttt aggcgctgta
     7501 tcaggaactt tcttttggag gttcttgctc tctagctcgt tgactctgtt tacaattcta
     7561 tcaaatgctt gttctgcact atcaacatac gcttcaaaaa cttttccttc ctgactagca
     7621 taactaccat tttcttctga ttgttctaag tgctctctgt attcacttct aataaactca
     7681 ttaccagagt acttttcaag atatggaaca acttgacgtg tcaaacgctc catcctgata
     7741 tggtttttaa agtcgagtaa tttatcataa ttttctttga ttttaggact atctttctca
     7801 agggtttgga cataactcat tagtttttgc ccgtcttcta aaaggtatgt ccatagtcct
     7861 ttttgaaatg aataatcaga gccagtgtag tctctaaata cctttaattc ttccaaatct
     7921 cgttctaact cttttagaat atcctgaaat tgtggaaggg gagcagactt aacttcggta
     7981 ttctctgata cttcttcctt agcttcgctt gtgagggctg ctggagcagg agttggttct
     8041 tctgattttt caggttcaga gactacattt tgttctgcag agttcgttgc tgtttcggct
     8101 ttttcagcaa ctggcgcttc ttccttaact tctggcgcaa cttttgattc agaatttgta
     8161 ttctctgtac ttactgttcc agtaatctgg actgttggct ctgtttcact cgttggctga
     8221 gctgcttcaa cttgagtagc tgttgaagtc tctgtcgtcg caaaagcggt tggtgtcgag
     8281 actcccgcac caagtaagca agtagcaatc agcaccgagc ctacgccgac tttaaattta
     8341 cgaatactat atctgaattt tttatttgca gtttccattt ctatcattcc taattatatt
     8401 ttattatata gaatcattat accacgatat tcgagtgact agatgtgttt tttatgtgtt
     8461 taaattctac tgaaagactc tgtttcttca tcgttgaata gatgtgacta tttttccaat
     8521 ggatgtataa tcacaccctg caccacacgg ttgacggtgc ctgttggtga tggtgtatct
     8581 ggcaagttgt tgcacttaat ggctgccatg atggataccg tttgggcaaa gaggatgtaa
     8641 gggaaggtta ggaagacatc tggcagatct gctccgcctt ctgccaagac aaagttggct
     8701 gcttctgtac cgactaactt atcagccgac aatgccacta tccgctctgc tatgccatca
     8761 ccatgaactt cattaagcaa gtccacatca tactgacgag tgtaggcatc gtttgaggca
     8821 aagaggaaaa tcaaggtctt ttcattgatg aaggattttg gtccatgacg gaaaccgagt
     8881 ggtgactcgt acatggtcgc aatcttacct gctgtcaatt ccagaatttt caactgagct
     8941 tcgtgggcaa gaccatagaa accacctgaa ccgaggtaaa tcactcgctc aaaatcaaga
     9001 ttaatcaagt cttgcacgta gtcttcacgg tccaaaacat cttgacccag acgggcaatg
     9061 atctcaaccc aagcagcttt ttcttcttga ctggctggcg agaagacgag gagggctgtc
     9121 agagccatac agctgtaaga acctgtcatg gcaaagccct tatcgtttga accagctggt
     9181 tgcaagagta ggagattttc aacatctcct tcagccgcta gtgccagctt gccttctggt
     9241 gcacaggtaa tggtcacttg atacaattta tctaccaatt gcttggcgac ttcaactgct
     9301 gccacacttt ctggtgaatt gcctgaacgg gcaaaggaca ccaggatggt tggaagatca
     9361 gcttgtagat agtcatgtgg acgactaacc agctccactg taccaatggc agaaaaacga
     9421 atgtgtttca agtcattgac ttggttgagg tagttggcaa tactttgccc aacaaagtca
     9481 gaagtccctg cacctgtaaa aacaacgtga acaaagtcat gtttttcctt gattgatttt
     9541 agaaaactct cgattttttc caattggtca aaatagagtt gatgcgcttc cttccataag
     9601 cctggctgtt ggtaaatttc ggtcgccgta atcatagctc ccagcttttc aagttcttct
     9661 tttgctaaac ggaacatagc tcttcctccg tgatttctgg ttataaccag ttttttctta
     9721 tattatagcg aatttacccc tcaaagtcaa tttaaaatag attaatatgt caaatttata
     9781 tccgatattg ccgactagaa cttattttga gaaggtagca ttatctacat aaaacatgtt
     9841 ataatattta ctacacctct gcctttgaaa atttttaaca ataaggcaaa ataaggcaag
     9901 gaaaaattac aaacaaggtc taaacccttg atacgaaagg aatcttaaaa atcttatgat
     9961 gaatatgcaa aacatgatgc gccaagctca aaaacttcaa aaacaaatgg agaagagcca
    10021 agctgagttg gctgctgctc aatttaccgg tagctctgta caggacttgg ttactgctac
    10081 ttttactggc gacaagaaat tggtttctat tgacttcaag gctgacgttg tagatgcgga
    10141 tgatattgaa actctgcagg aaatgaccgt ccaagccgtc aacgctgcac ttacaaaagt
    10201 cgacgaggcg actcagaaaa aactaggtgc ctttgcaggt aaattgcctt tttaaaaaga
    10261 aaacgcgatt ggatactctc ctctcgcgat ttttttattc aaaattataa atcaaagact
    10321 gaaattgctg gaaaaagagg tctggatagc gtcgattctc tttcatttcc tccatcagac
    10381 tttggaggtc ggtcatgttg tcagtgatga taccgtccac ttgtagcagc aacatcttgg
    10441 tcatactctc ttcttcattc ggtgtccaag cataaaccga cttccctcga atccaagact
    10501 tggtcataaa attttgatcc agcgaagtgt attccattgt ataaccatca gcaaccgtcg
    10561 tcggataaat tgaattgaac ggaagaataa agaaggatac taatttctcg ctatactgct
    10621 ttatttcgat aatagtacgg taatctaatg attgcatctg gtgtcctttg gcaatcaaac
    10681 gttgaccgta tttttttaag aaattctggg tgagttttgg cgaatctgcc ttggtcactt
    10741 taatttctac caataatttc tgtcccagct catccgcttt ttcaagatat tcatcaaaag
    10801 aaggaactgg agctaccatg ccgttttctg atgcagtcat ccctgtcaac tctgcaagag
    10861 tatagtcatg tgtacctccg ggattgcccg tcaaagccaa taaatccgta tcatgcatga
    10921 caataaactg accatccttc gtctcctgaa catccatctc aatataatct ggcttgagct
    10981 gagaggtttt ctctagggcc tcgatagagt tctggacagc attgccgtca tcgacacctc
    11041 ggtgagaaat ggtaaccggc agagtgtcaa agggaaagta gagggcaagc gctccttggg
    11101 taccgaacac cagaccagat acgagcaaaa tggctagtcg aaggtgatga cggaggcgtt
    11161 tacggcggca gattggtagt tgttttcctg ttaggaggct gacaaatccc atcatgaaga
    11221 gtgcaactac tccgtagtag gctaatttta gaagtgcgta acagacaact gccagccagt
    11281 aagaagcact tggtacaaaa tgctctgcta acagttgcac aagatagaat agtcctccta
    11341 ctcctgtaaa taggagaact ggaaaagtaa tcaaccaaat tagacggaac aatgaaccga
    11401 tctgctttct gcctctggtc ttctcccaag aataagcgat agactccttc acacccctgt
    11461 gttcaaaata aatgtttggc agagcgtaca tgaagcgtgc cgcaagccag aagaacaaga
    11521 ggaggaaaat caaaatcaat attcctagcc agactgtgtt ggataggtaa tctacgataa
    11581 attggggcac tacgatttta ttaaaataat agatatttaa tactcggcgt aaaaatggga
    11641 gaagaataac tgagtaaaag agcagaaata gaacctttgg taggctgctg tgacggataa
    11701 tcgccaccat actatctcgt acatcccgta aatagtcggg gaggtgcttg actttttcat
    11761 ccaacagttg ccaaacccct gtgaacaata gagctaattc tagataggca attgcaatgt
    11821 tgacaaaaaa gaggagaata aacaagcctg tcacgagtag attttcagca aacaccgaca
    11881 acaaattcgt cggtgaaagg taggcatgcc ctgtcatagc taagagtccc tcagaaatcc
    11941 atgaattgag cggtatccaa acaaattcca taaaggtaaa cagggtaaag aagagcaaga
    12001 gaatcttgtc taggttgtaa tatatttttt gaaattcacg ttgtatcttt ttcatgattc
    12061 tagtgtccca tattcttgca agaattgcaa gccttcatca aggatggaag attgtgaaag
    12121 caatgaatga agtatagaaa aaatcaagtt cctaagtgga gcttgatttc ttacatttga
    12181 tttgctttcc tatttccaag ccaaagtaaa tcgaaaggct acataaaata cgaatcacta
    12241 tctcactatt gttgtaacat taattgtgct tgtttaacaa tatattctgc tgtcaaacca
    12301 tatacatcca ttaagtaagc ttgttgccca acttgaccaa aagattcctc aataccaatt
    12361 cgatgtactg gggtcgttaa atctgtagcc attaattcac aaatgctact acctaggcca
    12421 ccaattcgat tatgattttc tacagttaat actggccttc ctgataatag agctggtatg
    12481 tcttcatgaa ttggtttgat acgaaaaaga tcaatgactt gaacagatac tccttgagcc
    12541 tctaactgat ccgccgcctt taatgcctgc tctaccataa taccactcgc aagcaaggtc
    12601 atatcctccc cttgcctaag gacacagtag cctttgctaa aatcttctcc accttgataa
    12661 atagccgttg tcgctttccg aatggtacga atgtacttca acccgtctac cttgatggtt
    12721 tctttaagaa cagcttgaaa ctggacatcg tcactgacct cataaacacg cgcttttgga
    12781 atgagccgca ttaagcccag ctcctcaaac ggcatgtgag tcccaccatt catctccgct
    12841 gatacccccg catcggaacc aatgaccgtg gcattaagtt gggcataagc tagagagata
    12901 aacacctgat catagactct acgactagca aatggtccga aggtatgaat gtatggcttg
    12961 tatcctagaa gagataatcc agctgctacc cctaccattt cctgctccat gatacctaca
    13021 ttgacatagc gtccacccag aacagaggac aatttgttgg tagccattga actggataaa
    13081 tctgcttcaa tagctgctat ggttgtgtct tcctgcccta cctgagctag aaaatcacta
    13141 taaaccagtc gcatctcctt tgtctgtctc attccatcac ctccaactct ctcgctaaaa
    13201 caagagttac ttgctctaaa tcttcccgta attgacccga caaacgcaaa tggtgattgc
    13261 tttctaattc ttctaagaag gtaactccct gccccttagt agtatcaaga acgatacatt
    13321 ttggttgagg agacttactt gacttcattg ttagaatagc atttataata gcatctatat
    13381 cggcaccatt cacacgcact gattcccaac caaaagcctg aaatttttca acaaaatcaa
    13441 aggtttggca aatatcatgg gtacgaccgt ctagttgttt tttattgtca tccacaaaca
    13501 gaatcatttt cgatagttcg tggtgagctg caaactgagc tgcttcccag cattgacctt
    13561 cgttaagctc cccatcccct accagagtat aggtatagta cggtgactgt tctatcttct
    13621 taccataagc aacacctgtc gcgacactca tcccttgccc caacgaaccc gttgtcatat
    13681 ccacccctgg cgttaagttt cggtcagggt gtgaaggcaa atgtgttcca ttttgattta
    13741 gcgacaagag aaaatctcta tcaaagaagc ctttcaaata caacgtacta taaagtgctg
    13801 gacctgcatg tcccttagac agaataaagt gatcccgctc cttagaagag aaattctgga
    13861 cacgaatgtc cagaatttct ccatatagag cagccaacac ttctacgatt gataagctac
    13921 caccataatg accgaatcct aattgattca aagtcgccaa ggtatggtag cgaatttctt
    13981 tggcaaatcg tttcaaatca gctgtaccac tagtcatatt atccttcttt ctgttctttt
    14041 ttcaccaatg acaatccaag catcaaagcc aagacagcaa agataccaac cgaaactgtg
    14101 aggataccgc cattcttagc tagcactccc aggaaaatac cagaaagacc aaagtctgca
    14161 tctgagaaag ttgaaccagc aaaaccaagg tctcccaaaa caggaagtaa gaatacaggt
    14221 aagaaactaa tgagaacccc ttgcataaaa gcaccacaaa ctgcgccacg aacaccacct
    14281 gacgcatttc ccatgacccc tgcagttgct ccacagaaga agtgtggtac gacacctgga
    14341 aggataacga caccgccagt caaggcaaga ataatcatgc tgacaatacc acctgcaaaa
    14401 ctagagataa agccaatcaa cacggcgttt ggtgcataag gataaacaac tggacagtct
    14461 agggctggtt tggaattcgg aaccaacttc tctgaaattc ctttgaaggc tggaacaatt
    14521 tccgctaaaa ttaagcgtac accagataag ataacaaaaa cacctgctgc aaaagttcca
    14581 cctagggtca aggcgtaaat catgctgtta gtaccaccac taaggttttc cttaatataa
    14641 tcaccgcctg caaacaaagc cacaatcatg taaatcaagg acatagaaat ggcaatgctg
    14701 acagttgaat cacgcaagaa tgccaaacct ttagggaaat taatatcctc agtagactta
    14761 gacttatcac cgaccaaacc gcctactaca ccactcaacc agtaaccaag tgcactgaag
    14821 tgacctaggg cgacatgatc atttcctgtc aattgaatca tgtatttttg aacgaaggct
    14881 ggggagagtg tcataatcag acctaaagcc aacccaccca tcacaattag gccgacagaa
    14941 gtgaatcctg caacactcat gataactgca atcatacagg ccatatatag tgtgtggtgt
    15001 cctgtcaaga aaatatattt aaagcgtgta aagcgagcaa tagcaatatt gaagaccatt
    15061 ccaagcaaca taatcaagga tgtagcagtt ccatacttat ccaaagccat cgctacaata
    15121 gcttcattat ttggaacaac ccctttcaag ttgaaagctt gttcaaacat ttgaccaaat
    15181 ggctcaagcg agccaacaat aatattcgct ccgccaccaa caacaataaa accaacaaaa
    15241 gttttcaagc cccccttaat taaatctgct gcgggctttt tctgtaaggc taaacctaga
    15301 atagcaatga gagcaaccag aattgccgga gttttagcaa tatcaatgag aaaatccaat
    15361 accattttct gtctccttta taattttatc tttgcgggtc atcccctgtg tatacgattt
    15421 aaaccaaacc tttttcttga cagatagccg tgacaagttc cctcaactca ttcatatcaa
    15481 taatactatt caatattcga acatctccta aatgagttgc agaatcagcc aggtcacgtc
    15541 cgacaatcca cacatctgct tcactcggat tggcacctcc tagatcataa tgctccacat
    15601 gaacatctga tacacctaaa tctcgcaaaa tggattcaat attcatttga accataaagc
    15661 tagagcctaa acctgaacca caggctgttc caattcgtaa catattactg ttcctccttt
    15721 aataaatgtt gtacgtcggc aaattctttg gcgccgacaa gttgttgaat attttcttct
    15781 gctcgcaaaa tttttgtcaa atgcgagagt gctctgagat gggtttgatt atccacggct
    15841 gcaatacaaa tgaacagccg aatttcatga ctagggtcat ccaatagata aactggctta
    15901 tccaatacca acattgacat ccctacctca ttaaccccat cttctggtcg tgcatgtgga
    15961 atcgcaattc ctttacctag gtcaataaaa ggaccaaact cttctacctt cgctatcata
    16021 gcatcaatat atcgagtttc tatagctcct ttatctacca gaggtcgtgc aaccagggca
    16081 actgcttttt gccagtctaa aacttcctca gaccattgaa aggtctcttt tgtaatcaat
    16141 tcttctaaca taggactcct tcccctttgc tcatgggagc gttgattgag aaactgcacc
    16201 aattcatatt ttaaagcctg ctcctgatga atggttgcat gtttcccaac aagtgataat
    16261 aactgctcaa tctcaatcgg aaaataagcc gcatttggga aatgctgatt aaccaagtga
    16321 aataattctt ttttatgatt agaggtcatc agctgggata ccaagaaaaa tggtagttct
    16381 atattcagct tgatagttcc aaataccatg tcatagtctg tcatgtctag catcttccaa
    16441 tcctgtaagg aatgggtatc ggcaaaatag atattaggaa atagttgccg tagattttct
    16501 tttacaatca gcgaagagct cactccgttt gggcaaacaa ccaaggcccg ataacgatat
    16561 gaacgttgtt gacctgcttc gatgtagcca ccaaagtgga tgacaaaata agatacctca
    16621 ctatctggta tagtaaaacc tagatgctct tccaaaggtc gcaaagcttt ttttacaaga
    16681 tagaacaagt cggcatgctc ttctttgatg gtttcagtat atgaattgac atttaccaaa
    16741 cgagatgtta aacggtagta agcaggaatt aggtgacgtt gcaaaccttc aatcatttcc
    16801 gagcgatgtt caaaagcaat taaggacaaa cgttccatct cttccactat ttctacagtc
    16861 aagtggtaaa atagatcatc cttttctccg ctctcacctt ctaagcatcc ttgcagtaaa
    16921 tgtgacagat actcttgtgt cgcagcattt aattctaatg tgctagtact ttttaaacgt
    16981 aataatttcc aaaggcgttg cagaaaatct tttacaaacc ctccctttcc ttcaaaattc
    17041 cagttaatac ggttgccaac acgcgcctga cggataaata aaaatatcaa aaaatacaaa
    17101 tattcatcca atctctcttc taaagctgag actttataat aattgcaagc ttgttgactc
    17161 actattttca attcttcaat aggattttcc tcaccccaag atttaagaat ataatccaaa
    17221 gcccacacac caatcgaaga ctgcaaacaa ttactgagtg cataaagcgc caagcgatgt
    17281 ttatcttctt ctcgaccaat tagatgatag cctttcgccc tcgtatattc caatcgtact
    17341 tgaaagtcca agcaaagttc ctttacattc ttaatatccg tcaatgttgt gttacggctc
    17401 acactcaata attcttggta gtgaacgttt gatacaaatt cttttcggat aaaggtataa
    17461 agatagataa gaagctgtct ttcttcttgc gaaagataga tctggcgatt gcgaaagagc
    17521 gaaaatacac tttctttttg attgtaaagc tccgtagaaa taacaaactc gctatctttg
    17581 tattgtatct gcgggaagga atgttcttgc aacaattgat tgactagttc caaattagaa
    17641 tgtagctgct ccttttcaat tcctgtttga atactaagtt cgtacaaact ttctttccga
    17701 gtcgcaaaca attccatcag aatcgccatg gtacgattat caaccatatt ttcctccttg
    17761 tcttaattgt atcccttttc atttctgatt tctagagtct ttgagcccaa ttaatataat
    17821 aaaaaataca aattcaattg tgctcactac ttaaactaaa aaagattggt tcccaccaat
    17881 ctttttatat cacatgccat cttccatttt ttagtgcggg ctgaaacagt ctatccctag
    17941 actgtacctc gctttcttat ttccaagctc gggctgaaac agtctatccc cagactgttt
    18001 cactcccccg gacctttatt cgctctcttg ctttcaagct catgataaaa aggtcccccg
    18061 gaccttttta tttcccaaac aaacgtgcaa agaagccttt agcttgcact tcttctgcct
    18121 gtgctttaac ttcgtccaat tccaacttga gaacttcctt atcggccata gcttttagtg
    18181 tcaattgctg ttgctgatcc aactgtttat ccttctcagc aatctgcaca tctttaacct
    18241 tgagttgttc atctttttcg gcaagttgct tatctttagc ttttaattga ctatataagc
    18301 ggatgatttc agcatttttc tcgtcaacta aaatttccat caattcacga tgcttgactt
    18361 catcactaat cggctcatct tcaaaaatgg ttgttttgta gatttcctcc aacttaatca
    18421 agccagcacg atttacaacg gtcacaccct tgtcattttt atctacaaat tcttcaggaa
    18481 gtgatttgac acgattattc atagcctgcc ggctcactcc taaaatttca gctaactcac
    18541 tgactgtttt ttcgattccc ataatatcct cggtacttac atttctcata gtttgtatca
    18601 ttaaaatatt accacagaac cctatccctg tcaaatctag gggaattttc ttaccaagca
    18661 ttagtgaata atcacaaacc tcaaaagcat cttatctcaa tgcaatgtta tgcagaaacg
    18721 aaatatacat tctatctaat aaaaccattt ttagcactca cttgcttgaa ccaatatcca
    18781 gattttttga cagtacgctt ctgagtttga aggtcaagcg agaccaaacc gtatcggttc
    18841 ttataggtat ttaaccaaga ccagcagtca ataaaagtcc agaccatata acccttacag
    18901 ttcgctcctt catcaatacc tcgatggagc tgaatcaaat gatcttccat gaagtgaatc
    18961 tgatagtcat cctgaatcat gccattttct tcaaaaacat cctccccttc aactcccatg
    19021 ccgttctcag ttaccaacca ttcgatattc ccatagtttt cctggatatt tttagcaatg
    19081 tcgtacagcc cttcttcata aatttcccag cctctatggg gattgatttt cttgtcaggc
    19141 ttatcataag gctggaaata ttgggatagg aaagtagctt ctccgttcca tccatcttct
    19201 gaaggagcct gcacgcgcag gggctggtaa taattcaccc ccaggaagtc aaccctattt
    19261 tctttgataa tggtcaattc ctcctctgtg gtttctggac gtaaaccgtg tttttcaaga
    19321 atgtcaacca gttccctcgg gtattgtccc aaaacagatg gatccaaaaa gcttctcgtt
    19381 tgaaatagct cagcaatccg cgctgcctgt aaatcctctg gactctgact tcttggatag
    19441 gcaggagtaa gattgagaac aatggaaatt ttgtggtatg ggtgtagttg gtgacaggcc
    19501 cgaacagcca aagaactagc cagttgagta tgatacgcta cgaccacagc tgcctttgca
    19561 tccaccttgc aaggataatg atagcttcct agatagccac attctactgg aacaatgggt
    19621 tcattaaagg taatccaatg atccaccaga tctccaaaga gactgaaaca ctggcgagca
    19681 taagcctcat aggcccagac aatctcctta ttttcccagc cccctttttc ctgtaagaca
    19741 taaggcaaat cgaaatgata aagattgacc aaaagcttta tcccacgctc tcgaatacct
    19801 gcaaatacag ccctataaaa ctccacagct tgcggattga cctcgcctat cccttccgga
    19861 atcagacgtg accactgtat agaggtgcga aaaatactat gacctgtttc caccaacaaa
    19921 tccaaatctg actggaaatg ttcataaaaa gtcgatgttt gttccagtcc tatttgctgg
    19981 tgaaagagct caggttcctg actgtaccag taatcccagt tattccaccc ctttccatca
    20041 ccagctacgc ttccttcact ttgaggacct gaagtggagg agccccacaa aaaaccttct
    20101 ggaaaacgat actctctttg ctcagttacc attcttctac ctactttctt tcatgctaca
    20161 gacggagttg ttagaacctg gattcacaat gcagcttcca caaaattact ttgcttgtac
    20221 atacaggctc ctgataaaaa ctaatttccc ttagtctaag tatactcccc taacccgtca
    20281 atggtcttcc gcaatctcaa ggaaaaactc cacaaatcat agcaaagact caaactactt
    20341 tcttgataaa aatatgatac aataaaggca tgaatacagt agataccatt attataggag
    20401 cgggccctgc tggcatgatg gccgccattt cttccagctt ttacggcaag aaaaccctgc
    20461 ttcttgaaaa aaacaagcgt ctgggcaaga aactgtctgg cacaggcggc ggacgttgca
    20521 atgtgaccaa caacggcaca ctagaagacc tgctggctgg cattcctggc aacggccgct
    20581 ttctttacag cgtcttttcc cagtttgaca accatgacat catgaatttc tttcaggaaa
    20641 atggggtcaa gctcaaggtg gaggaccacg gccgtgtctt tccgaccacc gaccgctccc
    20701 agaccattat caagtgcttg gagatgaaaa tgttggaaaa cggcgtcaat atccgcacag
    20761 ggactgaggt ggtttccgtc cgtaagattg atgacctctt ccatgtcaag accagcgagg
    20821 aaacctttac ggcacctcag ctcattgtca ccaccggtgg caagacctat ccctccaccg
    20881 gctcgactgg ctttggtcac gacattgccc gccacttcaa gctggaagtc acggagattg
    20941 aagccgcaga aagccccgtt ctgaccgact tcccccacaa gaagctccaa ggcatttccc
    21001 tggacgacgt caccctgacc tacggcaagc acgtcatcac ccacgacctg ctctttaccc
    21061 actttggcct gtcagggccc gctgctcttc gcttgtccag ttttgtcaaa ggcggcgaaa
    21121 ctgcatttct cgatgctctg ccgacccatt ctgaccaaga tctgtttgag catttagaag
    21181 ccaacaggga aaaatccgtc aagaatgccc tacgagaact catgccagac cgcctagccg
    21241 actttttcgc ggagaactac gactgcaagg tcaaacaggt ctcccaaaaa gacctgactg
    21301 atctggtctc ccttctcaaa gccctaccga tcaagattac cggcaagatg tccttggcca
    21361 agtcctttgt gaccaaggga ggcgttgacc tcaaggaaat caatccaaaa acattagaga
    21421 gcaagaaggt cccaggcctc cactttgccg gtgaagtctt ggacatcaac gcccataccg
    21481 gcggctttaa catcacctac tgtcttgcaa ctggttgggt ggctggaagt tcctactctt
    21541 cgtaaatcaa ac
//
LOCUS       DASFSW010000018        21544 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.018, whole genome shotgun sequence.
ACCESSION   DASFSW010000018 DASFSW010000000
VERSION     DASFSW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21544
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.018"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(121..1983)
                     /locus_tag="U1099_000550"
     CDS             complement(121..1983)
                     /locus_tag="U1099_000550"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEM4866894.1"
                     /translation="MKQSKMIIPTLREMPSDASVISHALMLRAGYVRQISAGIYSYLP
                     LANRVIEKAKNIMREEFDKIDAIEFLAPALLSADIWRESGRYETYGDDLYKLKNREGS
                     DFILGPTHEETVTLLARDAVQSYKQLPLNIYQIQSKYRDEKRPRNGLLRGREFIMKDG
                     YSFHASYESLDQTYDDYKAAYEAIFTRAGLEFKAIIGDGGAMGGKDSQEFMAITPDRT
                     DLDRWVVLDKSVASFEEIPEDVLEAIKEELLAWSVSGEDTIAYSSESGYAANLEMATS
                     EYKSSTAVVVEEELVKVATPDAKTIDEVAAFLNVAEEQTIKTMLFMADGEPVVALLVG
                     NDQVNDVKLKNHLGANFFDVAGPADAEKIFGAGFGSLGPVGLPENIKIIADRKVQDVK
                     NAVVGANEDGFHYTGANAGRDFQVTEYVDIREVKEGEPSPDGHGVLNFARGIEIGHIF
                     KLGTRYSDSMNANILDENGRSMPIIMGCYGIGVSRLLSAVLEQHARLFVNKTPKGEYR
                     YAWGINFPKELAPFDVHLIPVNVKDEAAMELTQSIEASLVGAGYEVLTDDRNERVGVK
                     FSDSDLIGLPIRVTVGKKAADGIVEVKIKGTGDTVEVHVDQLLETLQILTKENE"
     gene            complement(2267..3526)
                     /gene="rseP"
                     /locus_tag="U1099_000551"
     CDS             complement(2267..3526)
                     /gene="rseP"
                     /locus_tag="U1099_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEM4866895.1"
                     /translation="MKGILAFIFIFGVIVVVHEFGHFYFAKKAGILVREFAIGMGPKI
                     FAHTGKDGTLYTIRILPLGGYVRMAGWGEDKTEIKTGSPASLSLNEAGVVTRINLSGK
                     QLDSLSLPMNVTSFDFEEKLEITGLVLEESKTYKVDHDATIVEEDGTEVRIAPLDVQY
                     QNATVWGRLMTNFAGPMNNFILGILVFILLFFMQGGVANPSSNAVSITEGGALQAAGV
                     VTGDKILSVNGNTTDSYTEVATIISKAATDATTAPSFDLVVEHDGKNRHVSVTAEQVD
                     GAYRIGISPILKTGFVDKIIGGFQEAGATALRVVTALKNLIANFDVKQLGGPVAIYKV
                     SSQAAEFGLVSVLGLMAALSINLGIFNLIPIPALDGGKIVMNILEAIRRKPLKPETES
                     YITLAGVAVMVVLMIVVTWNDIIRVFF"
     gene            complement(3536..4342)
                     /locus_tag="U1099_000552"
     CDS             complement(3536..4342)
                     /locus_tag="U1099_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEM4866896.1"
                     /translation="MTNDLQKRVIFGGVALAIFIPFLLAGGMAFQFFVGLLAIIATAE
                     LIKMHRLAPNSIEGVLAMLASLVLALPLQNYLTFLPTDGNYTAYAIVVFLLLGSTVFN
                     IGHYNYSDVVFPIASSFYVGIGFHSLILARMDGLNKVFFALCLVWATDIGAYLIGRQI
                     GRRKLLPKVSPNKTVEGFAGGILSAVVVAIIFLIVDKSLLAGYSFGMMLLLVVIFSIF
                     SQFGDLVESAIKRHFGVKDSGKIIPGHGGILDRFDGMIFVFPIMHFFGLF"
     gene            complement(4352..5107)
                     /locus_tag="U1099_000553"
     CDS             complement(4352..5107)
                     /locus_tag="U1099_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEM4866897.1"
                     /translation="MFKFNFKKKESIETAIEVPKHIAVIMDGNGRWAKKRMQPRIMGH
                     KAGMDALQKVTKTASDLGVKVLTVYAFSTENWSRPEKEVKFIMNLPVEFYDKYVPELH
                     ANNVKIQMIGDHSKLPVPTLNALYKAEQKTKSNTGLILNFALNYGGRDEVTRAVKAIA
                     QDVLDAKFNPGDIDEKLIADYLYTGTLSPTLRDPDLVIRTSGELRLSNFLPWQTAYSE
                     LYFTDIAWPDFDGEALKLAIKEYNRRHRRFGGV"
     gene            complement(5221..5544)
                     /gene="yajC"
                     /locus_tag="U1099_000554"
     CDS             complement(5221..5544)
                     /gene="yajC"
                     /locus_tag="U1099_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEM4866898.1"
                     /translation="MEGLFLPLVMVAMIGFMFYSQRKQQKQRQEALNQIKKGDEIVTI
                     GGLFGIVDEIDDKKVVLDVDGVYLTFERGAIRNRVASSGTTSAVVEEVAVETTEATPE
                     AAIEE"
     gene            complement(5654..6946)
                     /locus_tag="U1099_000555"
     CDS             complement(5654..6946)
                     /locus_tag="U1099_000555"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEM4866899.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGTKYKLHLIPSGIFFPEKISVIGNGVVVNPKSLVKEINYLHDSGVTTDNLRISDRA
                     HVILPYHIKLDQLQEESKGENKIGTTNKGIGPAYMDKAARVGIRIADLLDKEIFAERL
                     RTNLAEKNRLFEKMYESTPIEFDEIFEEYYAYGQEIKKYVTDTSVILNDALDQGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELHDEIGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLETLKICVAYDLDGERIDHYPASLEQLKRCKPIYEEMPGWSEDITGVRSLDELPEA
                     ARNYVRRISELVGVRISTFSVGPGREQTNILESVWSAK"
     gene            7311..9542
                     /gene="gshAB"
                     /locus_tag="U1099_000556"
     CDS             7311..9542
                     /gene="gshAB"
                     /locus_tag="U1099_000556"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEM4866900.1"
                     /translation="MLQKLSPNSPILQATFGIERESLRINSNHRVAQTPHPHKLGSRS
                     FHPYIQTDYSEPQLELITPIAQSTKEARRLLGAITDVAARSMDKQEYLWPLSMPPVIS
                     EEEIQIAQLDSDYEYQYRVGLGERYGKLVQSMSGIHYNFELGKDLTQQLFELSKETDF
                     IAFKNTLYLKLAQNFLNYRWLLTYLYGASSLAEKGFLTTEVGCVRSIRNSKYGYVNSD
                     DVHISFSSLEQYVADIEQAVQSGQLSAEKEFYSSVRLRGAKTSRDYLSKGISYLEFRS
                     FDLNPYDPLAISQETLDTVHLFILSLLWLDQLTDVDNTLAKADKLNNLIALSHPHTPL
                     PNDADATPILTAMKAIVLHFGLDDYYGQLIAHVEAALQDPRLTLSGKIAEQIEDGSLE
                     KFGQQQGRLFHDYAWTAPYALKGYENMELSTQMILFDAIQLGLNVEILDEEDQFLKLW
                     HGDHVEYIKNGNMTSKDNYVIPLAMANKVVTKKILDQAGFPVPAGAEFANKTDALRYY
                     GQVASSAIVVKPKSTNFGLGISIFQEPASLADYEKALDIAFSEDSHVLVEEFVAGTEY
                     RFFVLDGKCEAVLLRVAANVVGDGSSTIRELVDQKNQDPLRGRDHRSPLEIINLGDIE
                     LLMLEQQGYTPDTVLPEGVQAFLRGNSNISTGGDSIDMTDQMDESYKQLAADMATAMG
                     AWACGVDLIIPDRTKPASKDKPNCTCIELNFNPAMYLHTYTYAGPGQSITPKILKKLF
                     PEL"
     gene            10099..13218
                     /locus_tag="U1099_000557"
     CDS             10099..13218
                     /locus_tag="U1099_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4866901.1"
                     /translation="MKIRNRSLFYTVGSVAVTAGLLFSLATSPIPSVHATEVAIENYP
                     SLAITKSEVTIQGYLIAPLNSSGTAFDATNKTNLALGASMDTAAADTIPIQLKEPLRS
                     QFNLVDHPELVGKLVRITGTSDTYMKRAGIKPATAIEIVDSSSTNVQPPTSENTATKP
                     SDLVSTPIATVRSGAQGTEYTVSGKIISLVNGWGGNGFYLQGSDGAGIYIYPGAALGY
                     QPGDTVQLTGTLGEYKGELQLTTVSNHKAISENFNTPITETNIAQLATQAQATLVSLK
                     NLTVGEIQSDSYQNSTFTVTDSEGQTVDVRLDSRTGIKTADLLNRINKGDKINLTAIL
                     STYNGKIQLKPFDLSHFEVIEKATTEAGLGKTEAVTVGRIQGASHQSPLVNQSVMLKN
                     VVVTYVTSANNFYVQDVTPDGDIKTSDGINIFTDKLKTNVKVGDLVTIAGRVEEYQGR
                     GYAERDKTDLTITQIRATEVTVNGTAPVPSPIVLGLDRTIPADIIDNDGLAQFDPEQD
                     ALDFWESVEGMVVAVDDAKILGPLKNKEIYVTPATSQLPLNNVGGVNLRPEGNNTNII
                     PLLLKNGKQIVKSGDYFTGRIAGPVTYSYTNYKVYVDDSTLPTLHEGATKPETTTIIP
                     NDDKLTIASYNIENFSADSKSTSDAKVQRIAKSFVSDLHSPDVIGLIEVQDNNGATND
                     GTTDASKSAERLIAAIQAAGGPTYTYVDIAPENNKDGGQEGGNIRVGFLYNSKRVSLS
                     DKPIGTATQAVAWENGELNLSLGRIDPTNPAWAAVRKTLAAEFVFKGEKVVVLANHLN
                     SKRGDNGLYGKIQPVSFKSEEKRHSLAQIITDFTKAGLTQNPNANIVMLGDFNDYEFT
                     KTIEILETGGMANLVSRHDASDRFSYFYNGNNQSLDNMLVSTNLLDRYAFDMVHVNSA
                     FMEEHGRASDHDPLLVQLDVTKAQEPTQPEPSDKQTDDSGTVNNSDDNGTTNNNKPTN
                     LSTSNQTAVNADDRSAATDKGQITVTPTANNSQKKILPKTGGETSFVLITIGLVFLSA
                     CLVKKQKES"
     gene            13298..14164
                     /gene="hslO"
                     /locus_tag="U1099_000558"
     CDS             13298..14164
                     /gene="hslO"
                     /locus_tag="U1099_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEM4866902.1"
                     /translation="MDKIIKTLSKSGHFRAFVLDSTETVKTAQEKHDTMASSTVALGR
                     TLIANQILAANEKGDTKITLKILASGAVGAIISVANTKGQVKGYIQNPDLDYKRTATG
                     EVIVGPLVGNGQFLVITDYGTGHPYNSMTPLISGEIGEDFAYFLTDSQQTPSAVGLNV
                     LLDEEDKVKVAGGFLLQVLPGATEAEIARFEKRIQEMPAISSLLASENHIEALLSAIY
                     GDDDFKRLSEEEIGFVCDCSKDRFLDALASLPKTDLQEMKEEDKGVDITCQFCQTHYH
                     FDENDLEELING"
     gene            14157..15161
                     /gene="dusB"
                     /locus_tag="U1099_000559"
     CDS             14157..15161
                     /gene="dusB"
                     /locus_tag="U1099_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEM4866903.1"
                     /translation="MANLNTPFMIGDVEIPNRCVLAPMAGVTNSAFRTIAKEMGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDDNEYPMSIQLFGGDAEGLKRAADFIQTNTKANIV
                     DINMGCPVNKVVKNEAGAKWLKDPDKIYHIIKEVTSVLDIPLTVKMRTGWNNTDLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTVDLETLTKVAGSLTKIPFIANGDIRNVEDAR
                     QRIEEVGADAVMVGRTAMGNPYIFNQINHYLETGEVLPDLSFEDKLEVAFDHLTRLTN
                     LKGESIAVREFRGLAPHYLRGSAGAAKIRGAVARAETIEQVQELFNQAREAYQERIAK
                     "
     gene            complement(15262..15753)
                     /locus_tag="U1099_000560"
     CDS             complement(15262..15753)
                     /locus_tag="U1099_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEM4866904.1"
                     /translation="MNQTIAVVFGTFAPMHKGHLDLIERAKLACGQVCVVVSGYDRDR
                     GDRIGLDLTKRFQFAQEQFKEDDFVEVRALDETDLPPYPDGWDLWLDRLLGLLDLSEH
                     QLPVFYVSEEEYAQELHSRGYQAHFSPRKFGISATLIREQPQQYQDYIAPAFLAFFGK
                     ETM"
     gene            complement(15871..18324)
                     /locus_tag="U1099_000561"
     CDS             complement(15871..18324)
                     /locus_tag="U1099_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEM4866905.1"
                     /translation="MKISTGLQRVFEDAQLVAQRYACDYLETWHVLLSFVINHDTVAG
                     AVLAEYPISISDYEHATFVVTDKVYREELDSFRILPSSKRLDETASFAKKIAEVVKAK
                     ELGTEHLFMAMLLDKRSTASQILDKVGFCFEDSDDKFRFLDLRKNLEARAGFTKEDLK
                     AIRSVMKGGKAKPTNMGQMMGMPPAPQSGGLEDYTRDLTALAREGKIEPVIGRDAEIA
                     RMIQILSRKTKNNPVLVGDAGVGKTALALGLAQRVAAGDVPVSLAKMRVLELDLMNVI
                     AGTRFRGDFEERMNNIINDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALA
                     RGTLRTVGATTQTEYQKHIEKDAALVRRFAKVTIEEPTVEDSIAILTGLKGTFEKYHR
                     VRIGQAAVETAVTYAKRYLTSKNLPDSAIDLLDEASATVQNRVKGQGEETGLTSIDKA
                     LMDKKYKTVSKLLIETKEDAEASQNYDLEVTEEDVLETLSRLSGIPVAKLSQSDTKKY
                     LNLEAELHKRVIGQEEAISAVSRAIRRNQSGIRTGRRPIGSFMFLGPTGVGKTELAKA
                     LAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLL
                     FDEVEKAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGF
                     GALDLSKDHKEVEKRIFEELKKAYRPEFINRIDEKVVFHSLTETHMQDVVKVMIKPLL
                     AVTAEKGITLKLQPSALKLLAKQGYDPEMGARPLRRLLQTKLEDPLAEMLLRGDLTTS
                     STLKVGVKGEELKFDVVKG"
     gene            complement(18328..18780)
                     /locus_tag="U1099_000562"
     CDS             complement(18328..18780)
                     /locus_tag="U1099_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEM4866906.1"
                     /translation="MKNTSDYIEEHIKALLEQVSVAELKRSELASRFEVVPSQINYVI
                     KTRFTASRGYIVESKRGGGGYIRIGRITFSDKHELVHDLLNNMGEQLSATVYADILQL
                     LFDEGLMTEREGNLFLFTGSDEILGKDASTIRARMMRQILRRLDRKEE"
     gene            18948..19307
                     /locus_tag="U1099_000563"
     CDS             18948..19307
                     /locus_tag="U1099_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEM4866907.1"
                     /translation="MFDTNHFFEDIKDFKAVSPKEASELLAAKDGAILFLGRDTCPYC
                     RRFAPKLAAVAAAQNWTVHFLHTQDPAYSAQEIAALREQYHVPTVSGLLVAKASGVDV
                     RCDSSLTEDEILAFVMA"
     gene            complement(19379..21247)
                     /locus_tag="U1099_000564"
     CDS             complement(19379..21247)
                     /locus_tag="U1099_000564"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017547.1,HMM:NF024296.1,HMM:NF037639.1,
                     HMM:NF037690.1,HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4866908.1"
                     /translation="MTKMKKILAFILLPVMILFSGYLGASSHVYGDETTNVITKMYFT
                     DKDGNELTPPNVEQWQQFRLNADFDIKNNAVKEGDVIEIELPSELIFNQSSDFQITDK
                     EGNVVANATITPEPEKKIKLTYTKYVEEHSDIKGSFYFYVRVDHRVIQQKKEIPLDIT
                     VQGKIIHAGKIQFQGIGTPDKRDIQKSGWSDSKNPQDAWFIIAINRSGQSMKDVKVVD
                     VLKSKQLEYIPDTLRVEKGNWSFKAGDWVLDNIETITDQVTLTFAPDGKSFSIELPDL
                     APTDGIQIIYKTKAPYVPVDGEEFSNEATLITTNGIDQTVTSGYTYLAAGGKAEGYVF
                     TIKVKKTDEAGAPLADVEFDVIRNRTGQSVGKITTDDQGEGSISNLLRDEYTLKEIKT
                     PDGYLPIEDMVINPAEFDGVTKSVLKEIVNKKKPAGPVKVVLKATKTLEGRALADQEF
                     EFTLLKKDDGSVVSTAKNDGTGVVTFDELTFDAPGTYKYTVKEVLGTDAGIDYDEVEY
                     DVIVTVTDNGGPTLVATVDKSEQEVNFINKVITTTTTTTTTTTTSEETTTTPETPAST
                     TTVETTVPEVPASTTTTGKILPKTGEKSGLWTSVLGVIFLVVGAGAVIFYRQSKEA"
BASE COUNT         6269 a         5253 c         4114 g         5908 t
ORIGIN      
        1 tttcagctca acaacttaaa acgaaaaatt gttgacgaac tctgtttgac ctagtcgagt
       61 tctttcccac tcccatttta tcaacaaaga cttggctatc atcaaccaag tctttttctg
      121 ttattcattt tcttttgtaa ggatttgcaa ggtttcaagg agttggtcca catggacttc
      181 tacagtatca cctgtgccct tgattttcac ttcgacgatg ccatcagctg ctttcttacc
      241 aacagtcaca cggattggca agccaatcaa gtcactgtca gaaaacttaa cgccgacacg
      301 ctcattacgg tcgtcagtta atacttcata gcctgcacca accaaacttg cttcgataga
      361 ctgagtcaat tccatcgccg cttcatcttt gacattaact ggaatcaaat gcacatcaaa
      421 cggtgccaat tctttaggga agttgatgcc ccaagcgtaa cggtattcgc cttttggtgt
      481 cttgttaaca aagaggcgag cgtgttgttc aagaacggct gacaagagac ggctaacacc
      541 gattccgtag catcccatga taatcggcat agaacgacca ttctcatcca agatgttggc
      601 attcatactg tctgaatagc gtgtaccgag tttgaagatg tgaccaatct cgataccacg
      661 ggcaaagttg agaaccccgt gtccatctgg agaaggctcg ccttccttga cttcgcgaat
      721 atccacatac tcagtcactt ggaaatcacg ccctgcattg gcacctgtgt agtggaaacc
      781 gtcttcgttg gcaccgacaa cagcattttt cacatcttga accttgcggt ctgcaataat
      841 tttgatgttt tctggcaagc cgactggtcc gagggaacca aaacctgccc cgaagatttt
      901 ttccgcatcc gctgggcctg ctacatcaaa gaaatttgct ccaaggtggt ttttcaactt
      961 gacatcgtta acctgatcat tgccaacaag gagggcaaca actggctcac cgtctgccat
     1021 aaagagcata gttttgatag tttgctcttc agcgacattc aagaaggccg caacttcgtc
     1081 gatggttttg gcatctggcg tcgctacttt aaccaattct tcttcgacga caactgctgt
     1141 acttgatttg tactcgctag ttgccatttc taggttggca gcataaccag attcgctaga
     1201 gtaggcaatg gtgtcttcac cagacacaga ccatgccaag agttcttcct tgattgcttc
     1261 aagaacatcc tctgggattt cttcaaaaga agcaacagac ttgtccaaga caacccaacg
     1321 gtccaggtca gtacggtctg gtgtaatagc cataaactcc tggctatcct taccacccat
     1381 ggcaccaccg tcaccgataa tggccttaaa ctccaagcct gcacgagtga agattgcctc
     1441 gtaagccgcc ttgtagtcat cgtaggtctg gtccagactc tcatagctag cgtggaagct
     1501 gtagccatct ttcatgatga actcgcgacc acggagaaga ccgttacgag ggcgtttttc
     1561 atcacgatac ttagattgga tttggtagat gttaagcggc aactgcttgt aagactggac
     1621 cgcatcacgc gccaaaagag tcaccgtttc ttcatgagtt ggtcccaaga taaagtcaga
     1681 accctcgcga tttttgagct tataaaggtc atcaccgtag gtttcataac gacctgactc
     1741 acgccaaata tccgcggaca agagggctgg tgccaaaaat tcaatagcat caatcttgtc
     1801 aaactcctca cgcatgatat tttttgcttt ttcaatcaca cggttagcca aaggcaagta
     1861 gctgtaaata ccagcagaaa tctgacggac atagcccgca cgtaacatca aagcgtggct
     1921 aatcaccgac gcatccgaag gcatctcccg caaagtaggg ataatcattt tagactgttt
     1981 catagttaag atataagtgc gttaagaaaa cgaaacgaaa ataggaaacc taacgcagtg
     2041 cttttggcac taggcaggtt tatctttttc gtaaagtttt tagcacgcat tcaattcctt
     2101 tcttatattc atcatcagga aatccagagg cgtagctctc aatctatttt ccgagttttt
     2161 agaccgagtt caattacgaa cctccttaat tcctttcatc ttttgtttat caggaatgca
     2221 caggtctcag cccgtgttca attcctttca taaatttcaa attatcttag aagaagactc
     2281 ttataatatc attccaagtg actactatca taagaaccac cataacggct acacctgcta
     2341 gagtgatata agattccgtc tctggtttga gtggttttct gcgaatggct tccagaatat
     2401 tcataacaat cttaccacca tccaaggctg gaattggaat caaattgaag attccaaggt
     2461 taatggagag cgcagccatt agccccaata cagaaaccaa accaaattca gctgcttgag
     2521 aacttacttt ataaatagca accggtcccc ccaactgctt aacatcaaag tttgcaatta
     2581 gattcttcaa agctgttaca accctaagag ctgtcgctcc cgcttcttgg aaaccaccga
     2641 taattttatc aacgaaacca gttttcaaaa tcggtgaaat accaattcga taggccccat
     2701 ctacttgttc agcagtaacg gagacatgac ggtttttccc atcatgctca acaaccaaat
     2761 caaagcttgg ggctgttgtt gcatctgtcg ctgctttgct aataatcgtt gcaacttctg
     2821 tgtagctgtc tgtcgtgttc ccattgaccg aaagaatctt gtcccctgtg acaacacctg
     2881 cagcctgcaa agcaccacct tcagtaatac ttactgcatt gcttgatggg ttggcaaccc
     2941 cgccttgcat gaagaacaaa agaataaata ctagaatccc taaaataaag ttattcatgg
     3001 gacccgcaaa gttggtcatc aaccgtcccc agactgtcgc attttgatac tgaacatcaa
     3061 gtggagcaat ccgaacttct gttccatctt cctctacaat cgtggcatcg tgatcaacct
     3121 tataggtctt gctttcttcc aagaccaagc ctgtgatttc caatttttcc tcgaagtcaa
     3181 aactggttac attcattggc aaactgagac tatccagttg cttaccagac aaattaatcc
     3241 gcgtcacaac accagcttca ttcaggctga ggctggcagg actacctgtc ttaatctctg
     3301 tcttatcttc accccatcca gccattcgaa catagccacc taggggaagg atgcggatgg
     3361 tgtaaagtgt cccatcctta cctgtatgag cgaaaatttt gggacccatc ccaatcgcaa
     3421 attccctcac aaggatacca gctttcttgg caaagtagaa atgaccaaat tcatggacaa
     3481 ccacaatcac accaaatata aatataaatg ctaaaattcc cttcataata cctccttaaa
     3541 ataatccaaa gaagtgcatg attggaaaga caaaaatcat accgtcaaaa cgatccagga
     3601 taccgccgtg tcctggaata atttttccag aatccttcac accaaaatgg cgtttaatag
     3661 cactttctac taaatctccg aattgtgaga atatcgagaa aatcacaact aacaacaaca
     3721 tcatgccaaa tgaatagccc gccaacaagg acttatcgac aatcaagaaa atgatagcaa
     3781 ctaccaccgc agaaagaata ccgcctgcaa agccttcaac cgttttattc ggtgaaacct
     3841 tcggcaataa ttttctccga ccaatctgac gaccgattaa gtaggcacca atgtctgttg
     3901 cccagactaa acagagggca aagaagactt tattgagccc atccatacga gccagtatca
     3961 aactatgaaa gccgatacca acatagaaac ttgatgcaat tggaaagact acatctgaat
     4021 agttgtaatg accgatatta aaaaccgtcg aaccaagcag taaaaatact acaatagcgt
     4081 aggctgtgta atttccatcc gtcggtaaaa atgtcagata attctggagc ggaagagcca
     4141 agactaaact ggccaacata gctaagacac cctcgataga atttggtgct aggcgatgca
     4201 ttttaatcaa ttcagccgta gcaatgatgg ctaacaggcc tacaaaaaat tgaaatgcca
     4261 tcccaccagc caacaaaaag ggaatgaaaa tagctagagc aacgccacca aatatcactc
     4321 gtttctgtaa atcatttgtc ataccttctc cttatacacc gccaaaacgt ctgtggcgac
     4381 ggttgtattc tttaatcgct aatttaagtg cttctccatc aaaatcaggc caagcaatat
     4441 ctgtaaagta cagctcgctg taggctgttt gccacggcag gaagttgcta agacgaagtt
     4501 caccacttgt acggataact agatcgggat cgcgtaacgt tggtgataag gttcctgtat
     4561 agaggtaatc cgctattaat ttctcatcaa tatccccagg attgaacttg gcatccaaga
     4621 catcttgcgc aatagccttg actgcgcgag tcacctcatc acgaccgcca tagttaagag
     4681 cgaaattgag aatcaaacca gtgttactct ttgtcttttg ttcggcttta taaagcgcat
     4741 tgagagtcgg gacaggaagt tttgaatggt caccaatcat ctgaattttg acattattgg
     4801 catggagttc aggaacatac ttatcataaa actccacagg tagattcatg ataaacttca
     4861 cttctttttc cggtcgagac cagttttctg tagaaaaggc atagacagtt aacaccttta
     4921 ctcccaaatc agaagccgtc ttggtaacct tttgaagtgc atccatacca gccttatgcc
     4981 ccataatacg tggctgcatc cgctttttcg cccaacggcc attgccatcc ataatcacag
     5041 cgatatgctt agggacttca attgcagttt caatgctttc tttcttttta aaattaaatt
     5101 taaacatggt attttcctta ttgtataatc gtttcattat accatatttt tacctaggat
     5161 agcaaaaatc aggcagggct aaaacaagag aaaaccaagg tttccctcgg tttttcatcc
     5221 ttattcttca attgcagctt ctggagttgc ttctgtcgtt tcaactgcca cttcttcaac
     5281 aactgcagat gtagtacctg aacttgctac acgattacga atagcaccac gttcaaatgt
     5341 caagtaaaca ccgtcaacat cgagaacaac ctttttatca tcgatttcat cgacaatacc
     5401 aaatagccca ccaatggtca caatttcatc accttttttg atttgattta gtgcttcttg
     5461 acgttgtttt tgttgtttac gttgcgaata gaacataaaa ccaatcatgg caaccatgac
     5521 aagcggcaaa aataaacctt ccatttgttt acctctttta ttgtttgata agcatgatta
     5581 tatcaaattc tggacaagaa agcaaagttc gaccaaacag aaaaagaccg aactttcgtt
     5641 cagtcttcgc acactatttt gcgctccata cactctcaag gatgttggtc tgttcacgac
     5701 caggtcctac tgagaaggtt gagatacgaa cgcctaccaa ttcgctgata cgacgaacat
     5761 agttgcgagc cgcttctggc aattcatcca ggctacgtac acctgtgatg tcttcagacc
     5821 aacccggcat ttcttcgtag attggtttgc aacgtttgag ttgctccaaa cttgctgggt
     5881 agtggtcaat acgctcacca tccaagtcat aagccacgca gattttcaag gtctcaagac
     5941 ctgacaagac gtcaatcgag ttgagggaca agttggtaat acctgacaca cggcgactat
     6001 ggcgcatcac cactgagtca aaccaaccga cacggcgtgg acgcccagtt gtcgtaccgt
     6061 attccttacc gatttcgcgg atacggtctc cgatttcatc gtgcaattca gtcgggaatg
     6121 gtccgtcacc aacacggcta gtgtaggcct tacatacacc aacaacctta tcaatcttgc
     6181 ttgggccgac accgctaccg atcgtcacac caccagcaac tgggtttgaa gaagtaacga
     6241 atggataggt accttggtca atatccaaca taaccccttg cgcaccttca aataagacac
     6301 gcttgccttg atcgagggca tcgttcaaga taacagaggt atctgttaca tactttttga
     6361 tttcttgacc ataagcgtag tattcttcaa agatttcgtc aaactcaatc ggagttgact
     6421 catacatttt ctcaaacaaa cggtttttct cagccaagtt tgttctcaaa cgctccgcaa
     6481 aaatctcctt gtccaagagg tctgcaatac ggataccgac acgcgcagcc ttgtccatgt
     6541 aggctggacc gatacccttg ttggttgtac caatcttatt ctcaccttta gactcttctt
     6601 gcaactggtc caatttgatg tgataaggca agatgacatg tgcgcggtct gaaatccgca
     6661 aattatctgt tgtcacacct gaatcatgga gatagttgat ttccttcacc aaagattttg
     6721 gattgacaac cacaccatta ccgattacag aaatcttttc tgggaagaag attcctgacg
     6781 gaatcaagtg caatttatac ttcgtgccat cgataacgat agtgtgtcca gcgttatcac
     6841 caccttgata acgtgcgatt acttcagcat tagcagacaa gaagtctgta attttaccct
     6901 taccttcgtc gccccactgg gttcctacaa caactactga tgtcatattc actctttctc
     6961 tgaactggct aaccagccca gcctttcttc tacttctggc aggaatctca cctgcaagtt
     7021 tcgttttaca cactattcta tcaaaaaacg aacatttttt caagaaattc caataatttc
     7081 atatatatcg aatgttttta tttttttaaa agtatatttt aaaaaaatcc gattaaacac
     7141 acgaggtaga catgtgtaaa tatgcttttg gcagagaaat ccgaagtctt tgtttacttt
     7201 cagtgatttt cagtacattt ttcaccagaa acgtagcaga aatttgtatt tgctctccaa
     7261 tctagataaa attggaataa gactttgaca gaaatataaa ggacttagat atgttacaaa
     7321 aattatcacc aaacagcccc attcttcagg ctacttttgg aattgagcgc gaatcacttc
     7381 gcatcaattc aaaccacaga gttgcacaaa ctccccatcc tcataaactg ggttctcgta
     7441 gcttccaccc ctatatccag acagactaca gcgagccaca gttagagttg attacaccaa
     7501 tcgctcagtc gacgaaggaa gcgcgtcgtc tactgggagc cattacagat gttgccgcac
     7561 gttctatgga taagcaagaa tacctctggc ccctatccat gccacctgtg atttcagaag
     7621 aagaaatcca aattgctcaa ctagatagcg actatgagta ccaatatcgt gtcggtttgg
     7681 gggaacgcta tggcaagctc gtccagtcta tgtccggcat ccattataat tttgaattag
     7741 gaaaagactt aacccaacaa ctttttgaat taagcaagga aacagacttt atcgcattca
     7801 aaaataccct ctacctcaaa ttagcacaaa acttcctcaa ctatcgttgg ctcttgacct
     7861 acctatatgg agcaagtagc ctagccgaaa aaggattcct aaccacggaa gtcggttgcg
     7921 tccgttccat tcgtaattcc aagtacggct atgtcaactc cgatgatgtt catatttcct
     7981 tttcttctct cgaacagtac gtggccgaca tcgaacaagc tgtccaatct ggtcaactat
     8041 ccgctgagaa ggaattttac tcttccgtcc gactacgtgg agcaaagact agccgtgact
     8101 acctcagcaa ggggatttct tatttagaat tccgttcctt cgaccttaac ccctatgacc
     8161 cgcttgctat tagtcaagaa acccttgata cagtccacct ctttatctta tctcttcttt
     8221 ggctagacca actgacagat gttgataaca ccctagcaaa agcagataaa cttaacaatc
     8281 tcatcgccct cagtcacccc cacacacctc tacctaacga tgccgatgcc acgccaatct
     8341 tgacggccat gaaagccata gttctacact ttggactgga tgactactat ggccagctca
     8401 ttgcacatgt agaggcagca cttcaagacc ctcgtttgac cctttctgga aaaatagcag
     8461 agcagattga agatgggtct ctagaaaagt tcggccagca gcaaggccga ctcttccatg
     8521 attatgcttg gacagcccct tacgccctta agggctatga aaacatggaa ctctccaccc
     8581 agatgattct ctttgacgcc attcagctgg gcttgaatgt ggagatttta gacgaggaag
     8641 accagtttct caaactctgg catggtgacc atgtcgagta catcaaaaat ggtaacatga
     8701 cctccaagga caactacgtc attcctctcg ccatggccaa caaggtcgtg accaagaaaa
     8761 tcttggacca ggctggtttt ccagtacctg cgggagctga atttgcaaac aagacagacg
     8821 ccctgcggta ttacggacag gtagccagct ctgctattgt tgtcaaacca aagtccacta
     8881 acttcggtct cggcatctct atcttccaag aaccagccag ccttgcggac tatgaaaaag
     8941 cccttgacat cgccttttca gaagacagcc atgtactagt ggaggaattc gtggcaggaa
     9001 ccgagtaccg tttcttcgtc ctggacggca agtgcgaggc tgtcctgctc cgcgtcgcag
     9061 ccaatgtcgt gggggacggt agctcaacca tccgtgaatt ggtggaccaa aaaaaccaag
     9121 atccactgcg tgggcgtgac caccgttcac cgctggaaat cattaacttg ggcgacatcg
     9181 aactgcttat gttggagcaa caaggctaca cacccgatac agttttgcca gagggcgttc
     9241 aagcctttct gcgtggcaat tccaatatct caacaggtgg tgactccatc gatatgaccg
     9301 accagatgga cgagtcctac aagcaactgg ccgctgatat ggcgactgct atgggagctt
     9361 gggcttgcgg tgtggacttg attatccccg accgcaccaa gccagccagc aaagacaagc
     9421 ccaactgcac ctgtattgag ctcaacttca accctgccat gtacctgcac acctatactt
     9481 acgcaggacc tggacagagc attacgccga agattttgaa aaaattgttt ccagagctat
     9541 agttttccat tttccttact ttgttagtac ttacttcaaa gattcgtgga gcttttgaag
     9601 gctggagata gggcaaagcg agttcgcctc aatagcggca ggttgctaga acattctagg
     9661 ttggaaataa gacttgcctc tctataaagt acggcaaagc gagttcaaac aaaccagtgg
     9721 attgtttgaa gtgggaaagc cagaaacggg gttcctcttg cgtctaagta aaaaagtcaa
     9781 ctaaaggact atatatctgc tttgcccctt tgttccttca cgaaaactcc caactaatgt
     9841 taaacaaaac gaataagaca ccgagataat catttatatc tcggtgcttt tttgtgaact
     9901 aattcccatc actcaaccgt tttgttcgcc ttttattttg ttttccaatt tatttttatt
     9961 atttcatttg attttttgaa aatatccgta ttatatctaa aaaaagtatt ccaaacgact
    10021 cattttctgt taaactatat gtatataaca actgcagacg tttttatgac gtccatatat
    10081 aacaaaaagg agttttatat gaagattaga aaccgttctc tcttctatac cgtaggttct
    10141 gtagcggtaa cagccggact cttgtttagt cttgctactt cgcccatacc atctgtgcac
    10201 gctactgaag tggcgataga gaattatccg tcacttgcaa taaccaagtc ggaagtcacg
    10261 atacaaggct atcttattgc accacttaat agtagcggaa ctgcttttga cgctactaac
    10321 aaaacaaatc tagcccttgg tgcaagcatg gatactgcgg ccgccgacac tatccctatt
    10381 caactaaaag aacctcttcg tagccaattc aacctcgttg atcatcccga actagtcgga
    10441 aaattagttc gtatcacagg aactagcgat acatacatga agcgagctgg gatcaagcca
    10501 gctacagcga ttgaaattgt agattcatct tctactaatg ttcagccacc aacaagtgaa
    10561 aatacagcta ccaaaccaag tgaccttgtt tctacaccga ttgctactgt tcgttccgga
    10621 gcacaaggaa cagagtacac agtttctggt aaaattatta gcctagtaaa cggttgggga
    10681 ggaaatggtt tttacctcca gggttctgac ggtgctggta tctatatcta cccaggggct
    10741 gccttgggct atcagcctgg tgatacagtc caattaacag gaacattggg tgagtataaa
    10801 ggcgaactcc aactaaccac agttagcaac cacaaggcta tctctgagaa tttcaacact
    10861 cctattacag aaacaaacat cgctcagtta gcaacgcaag cacaggcaac actagtttct
    10921 ctaaaaaact tgactgtcgg agagattcaa agcgatagct atcaaaattc tacctttacc
    10981 gtaactgatt ccgagggaca aactgtggat gtgcgtttag atagtcgaac aggcatcaaa
    11041 acggcggatc ttttaaaccg gatcaacaag ggagataaga ttaatctaac cgctatttta
    11101 tccacctata atggaaaaat acaattaaaa ccatttgacc tctctcattt tgaagtcatt
    11161 gaaaaggcaa cgacggaagc aggactagga aagaccgaag ctgtaacagt cggtcgcatt
    11221 caaggtgcaa gtcaccagtc acctctggtg aatcaatctg ttatgcttaa aaatgtcgta
    11281 gtaacctacg ttacctctgc caataacttc tacgtgcaag atgtcacacc agatggagac
    11341 atcaaaacat ctgacggcat taacatcttt actgacaaat taaaaacaaa tgtcaaggta
    11401 ggagatcttg tcactatcgc aggacgagta gaagaatacc agggcagagg gtatgctgaa
    11461 cgtgacaaaa cagacttaac catcacacaa attcgagcta ctgaagtaac tgtgaatggg
    11521 actgcacctg taccatctcc tatagtcctt ggtcttgatc ggaccatccc agcagatatt
    11581 attgacaacg acggtttggc tcaatttgat ccagaacaag atgcgctcga tttctgggaa
    11641 tccgttgaag gcatggtggt tgccgtggat gatgcaaaaa tccttggccc actaaaaaac
    11701 aaggaaatct atgtcactcc tgccactagt caacttcctt taaataacgt tggaggcgtc
    11761 aaccttcgcc ctgaagggaa taataccaat atcattccac tccttttgaa aaatggaaaa
    11821 cagatcgtca aatcaggaga ctatttcacc ggtcgtatcg ctggaccagt tacctactct
    11881 tacaccaatt ataaagtcta tgtagatgat agcacccttc caaccttgca cgaaggagct
    11941 acaaaaccag aaacaacaac cattatccca aatgatgaca agctgactat cgcttcttat
    12001 aacattgaaa acttttctgc tgacagtaag tcaacatcag acgctaaagt ccaacgcatt
    12061 gccaaatcct ttgtttcaga cctacattct ccagatgtta tcggattgat tgaagtgcag
    12121 gacaataacg gtgctacaaa tgacggcaca actgatgcta gtaagagtgc tgagcgccta
    12181 attgcagcca ttcaagcagc tggcggacca acctatacct atgtggatat tgctcctgaa
    12241 aataacaaag acggcggaca ggaaggtggc aatattcgcg tcggcttcct ctacaactca
    12301 aaacgtgtca gcctatctga caaaccaatc ggtaccgcaa cacaggcagt tgcatgggaa
    12361 aacggcgaac tcaatctcag cctgggccga atcgacccaa ccaaccctgc ttgggcagct
    12421 gtccgcaaaa cacttgcagc tgaattcgtc ttcaaaggcg agaaagtcgt tgtccttgcc
    12481 aaccatctca actcaaaacg cggtgacaat ggtctctacg gaaaaatcca acctgtcagc
    12541 ttcaagtccg aagaaaaacg ccactcacta gcacaaatta tcactgactt tacaaaagca
    12601 ggtttaactc aaaatccaaa tgctaatatc gttatgctag gcgacttcaa tgactatgaa
    12661 tttactaaaa ccatagaaat tttagaaacg ggaggaatgg ctaacctcgt tagtcgtcac
    12721 gatgcatctg atcgcttctc ttacttctat aacggaaaca atcagtctct tgacaatatg
    12781 ctagtatcaa ctaacttgtt ggaccgctat gcctttgata tggtccatgt caattcagcc
    12841 tttatggaag aacatggcag agcttctgac cacgatccgc tattggtaca actggatgta
    12901 acaaaggcac aagaaccaac tcaaccagaa ccaagcgata agcagacaga tgactcagga
    12961 acagtcaata atagtgatga caatggtacg accaacaata acaagccaac aaatctttca
    13021 acttctaatc aaacagctgt aaatgctgat gaccgttctg ccgctacaga caagggacaa
    13081 ataaccgtga cccctactgc caacaacagt caaaagaaaa tccttccaaa gacaggagga
    13141 gaaacaagct ttgtacttat cacaatcggt ctcgtattct tgagcgcctg ccttgtcaaa
    13201 aaacaaaaag aatcctaatc attttttgag cttgaaagca tgactttcag gctcattttt
    13261 ctactgctcc acctccactt gtgctataat taaagttatg gataaaatta ttaaaacact
    13321 atcaaagagc gggcatttcc gtgcctttgt gctagatagc acagaaaccg tgaaaacagc
    13381 ccaagaaaaa cacgacacca tggcgtcgtc caccgttgct ctggggcgca ccctcattgc
    13441 caatcagatt ttggctgcca atgaaaaagg cgacaccaag atcaccctga aaatcttggc
    13501 cagcggtgct gttggggcta tcatctccgt tgccaatacc aaaggtcagg tcaagggcta
    13561 cattcaaaat ccagacttgg attacaaacg gacagcgact ggcgaagtca tcgttgggcc
    13621 tcttgtaggg aacggacaat tcctggttat cacagactac ggaacaggtc atccttacaa
    13681 ctccatgaca cccttgattt ctggtgaaat cggtgaagac tttgcctact tcctaacaga
    13741 cagccagcag acaccgtctg cggtggggct gaacgtcttg ttggacgaag aagacaaggt
    13801 caaggtagct ggtggcttct tgttgcaagt tctgccaggt gcaaccgagg ctgaaattgc
    13861 ccgtttcgag aaacgcatcc aagaaatgcc tgccatttcg agtttgctgg cttctgaaaa
    13921 ccatatagaa gctctgctat ccgctattta tggcgacgac gacttcaaac gactgtcaga
    13981 agaagaaatc ggttttgtct gcgactgctc taaagaccgc ttccttgatg ctctagctag
    14041 tctgccaaaa accgacttgc aagagatgaa ggaagaagac aagggcgtgg acatcacctg
    14101 tcaattctgc cagacccatt accactttga cgaaaacgat ttggaggaac tcatcaatgg
    14161 ctaatctcaa cacccctttc atgattggtg atgtggaaat ccccaatcgc tgcgtgctgg
    14221 ccccaatggc cggcgtgacc aactcggcct tccgcaccat tgccaaggaa atgggcgcag
    14281 gcctggtcgt tatggaaatg atttctgaaa aaggccttct ctacaacaac gagaagaccc
    14341 tccatatgct ccatattgac gacaacgaat accctatgtc catccagctt tttggcggcg
    14401 atgccgaagg actgaaacgg gcggccgact tcatccaaac caacaccaag gctaatatcg
    14461 tagatattaa catgggctgc cctgtcaaca aggtcgttaa aaacgaagct ggtgccaagt
    14521 ggctcaagga ccccgacaag atttaccaca tcatcaagga agtgacctca gtcctcgata
    14581 ttcccttgac tgttaaaatg cggacgggtt ggaacaatac cgacctagcg gtcgaaaatg
    14641 ccctggccgc agaaagtgcc ggtgtgtctg ccctggccat gcacggacgg acccgcgagc
    14701 agatgtacac aggaaccgtt gacctggaaa ccctcaccaa ggtggctggc agcctgacca
    14761 agattccttt catcgcaaac ggcgacatcc gcaatgtcga agatgctcgt caacgtatcg
    14821 aggaagtcgg agctgatgcc gtcatggtcg gacggactgc catgggcaat ccttatatct
    14881 tcaaccaaat caaccactat cttgaaactg gagaagtcct acccgacctc tcctttgaag
    14941 ataaactcga agtcgccttc gaccacctga cccgattgac caatctaaaa ggcgaatcca
    15001 tcgccgtacg tgaattccgt ggtcttgccc cacactacct ccgtggttca gcaggtgctg
    15061 ccaagatccg tggcgccgtt gcacgagcag agacgattga acaggtccaa gaactcttta
    15121 accaagctag agaagcttat caagaacgga ttgctaaata agaaactctg agaagtttga
    15181 tacttgccaa ccatcaaaca tatataaaaa cgcataaaac caaggttata acctgatttt
    15241 atgcgttttt tctatactgt tttacatagt ttcctttcca aaaaaggcaa gaaaggctgg
    15301 agcaatgtaa tcctgatatt gttgaggttg ttcacgaatg agggtcgctg aaataccaaa
    15361 ttttctagga ctgaaatgcg cttgatagcc tcgactgtgc agttcctgag cgtattcttc
    15421 ctcagacaca tagaaaactg gtagttgatg ttctgacaag tccaataaac caagcaggcg
    15481 gtctagccaa aggtcccaac catctggata gggaggcaag tccgtttcat ccagggcacg
    15541 aacttcgaca aaatcatctt ccttaaactg ctcttgagca aattgaaatc gtttggtcag
    15601 gtctaaacct atcctatccc cacgatccct atcatagcct gaaaccacca cacaaacctg
    15661 accacaggcc aactttgctc gctcgatcaa atccaaatgt cccttgtgca tgggggcaaa
    15721 agttccgaaa acaacagcta tggtttgatt catagatttc ctttctactt gctaaagagg
    15781 cgagcaagct cacctctcca actatttcat ccttttttca aaacggatga acaattctgc
    15841 atctggaaag gatagcaaca caagcactcc ttatcccttt accacatcaa acttgagctc
    15901 ttcacccttg accccaacct tcaaggtcga actagtagtc aagtcaccac ggagtaacat
    15961 ttctgccaac ggatcttcca atttggtttg taagagccta cgtagcggac gagcccccat
    16021 ttctgggtca taaccttgtt tagccaataa cttaagggct gacggttgca gtttaagagt
    16081 aattcctttc tcagctgtta cagccaacaa aggtttaatc atgaccttga caacatcctg
    16141 catatgagtt tccgttaggc tatggaagac aactttctcg tcaatacggt tgataaattc
    16201 tggacgatag gcttttttca attcctcaaa aatgcgtttc tccacttcct tgtggtcctt
    16261 agataaatca agggcaccaa aaccaactgt cttatcatca cgaagagctg ttgctccaag
    16321 gtttgacgtc atgataataa tagtatttga gaaatcaacc ttacgtccct tactgtccgt
    16381 cagaacacca tcatccaaga cctgcaagag aacattgaaa atatctggat gagccttttc
    16441 aacctcatca aaaagaagaa cagaataagg tttgttacga actttttctg tcaattcgcc
    16501 accttcttca tagcccacgt aacctggagg cgctccattg agacggctgg ccgcaaattt
    16561 ctccatatac tcggacatat caaaacggat aagagcagat tcatcatcaa agagaacctc
    16621 tgccaaggct ttagcaagct ctgtcttacc gacaccagtt ggtcccaaga acataaagga
    16681 accaatcggt ctgcgacctg tacgaatccc tgactgatta cggcgaatag ctctgctaac
    16741 tgcagaaata gcttcttcct gcccaatcac acgcttgtgc aattctgctt ccagattcag
    16801 atacttctta gtatctgatt ggctaagctt ggctactggg atacctgaca agcggctaag
    16861 tgtttctaaa acatcttctt ctgttacttc aaggtcatag ttttgactag cctcggcatc
    16921 ttctttcgtt tcaatcaaaa gcttgctgac agtcttatat tttttatcca tcaaggcctt
    16981 gtctatgctc gtcagtcctg tttcctcacc ctgccctttc actcgatttt gtaccgttgc
    17041 actggcttca tccagcaagt caatggcaga atctggcagg tttttactgg tcaaataccg
    17101 cttagcgtag gtcacagcag tctctacagc tgcttgtcca atacggaccc tgtggtattt
    17161 ttcaaaagta ccttttaaac cagtcaaaat ggcaatgcta tcttccactg tcggctcttc
    17221 aatggtcact ttggcaaaac gacgaaccag ggctgcgtct ttctcaatat gcttttggta
    17281 ctctgtctga gtcgtcgctc cgaccgtacg gagggtgccc cgagcaagag ctggtttgag
    17341 gatattggct gcatccaagg tcgaatcaat ccctgagcca gaacccataa tggtatgcaa
    17401 ctcatcgatg aaaaggatga cctgaccatc ttcctcgatg tcgttgatga tattattcat
    17461 ccgctcttcg aaatcaccac ggaaacgagt tccagcgata acgttcatca agtcaagttc
    17521 caaaacccgc atcttagcca agctgacagg gacatcacct gcagcaacac gttgagcaag
    17581 acccaaagca agcgcagttt ttccgacacc agcatcacca actagaactg ggttattctt
    17641 ggttttacgt gatagaattt ggatcatccg tgcaatctca gcgtctcgac cgatgacagg
    17701 ttcaatcttc ccttcacgcg ccaaagctgt caaatcacga gtgtagtctt ctaatccacc
    17761 actttgaggt gctgggggca ttcccatcat ctgtcccata ttagtaggtt tggctttgcc
    17821 acccttcatc acactgcgga tagctttaag gtcctctttg gtaaatcccg cacgtgcctc
    17881 caagttttta cgcaaatcca agaaacgaaa cttatcatct gagtcttcaa aacaaaaacc
    17941 taccttgtcc aaaatctgac tagctgttga ccgcttgtcc aacaacatag ccataaacaa
    18001 atgttccgtt cccagctcct tagccttgac gacttctgca attttcttgg caaaactagc
    18061 cgtttcatcc aaacgtttag aagatggtaa aatacgaaaa ctatccaact cttcacgata
    18121 gaccttgtcg gtcacaacaa aagttgcatg ttcatagtca gaaattgaaa taggatattc
    18181 tgccaaaaca gcgccagcga ctgtatcatg gttgataacg aaagacaaga gaacatgcca
    18241 ggtttccaag taatcacagg cataacgttg agccactaac tgggcatctt caaaaacccg
    18301 ctgtaaacca gttgaaattt tcatgaatta ttcctctttc ctatctaaac gtcgtagtat
    18361 ttgacgcatc atgcgtgctc gaatagtact tgcatctttt cctaatattt catcagatcc
    18421 tgtgaacaaa aagagattgc cttctcgttc tgtcatcaat ccttcatcaa aaaggagctg
    18481 taaaatatcc gcataaaccg tagcagacaa ctgctcaccc atattgttta acaaatcatg
    18541 gaccagttcg tgcttatccg aaaaggtaat ccgcccaatt cggatataac caccaccacc
    18601 acgcttactc tcaacaatat agccacggct ggctgtaaaa cgggttttaa tgacgtagtt
    18661 aatctgactc ggtaccactt caaatcggct cgcaagttcg ctccgtttta gctccgctac
    18721 actcacctgt tccaagagag ccttgatatg ctcctcgata taatccgacg tgtttttcat
    18781 acacttccct ttctaactag actgactatt tttgactatt gtcttattat accgaaaaat
    18841 cacccatttg gaaagcaaag acaccgtttc tacaacgtat caattttttg ctttctcccg
    18901 tcaaatagct tatactaaac ctattacaat gaaatggaga ataccccatg tttgatacca
    18961 atcatttttt tgaagatatc aaagatttca aagctgtctc accaaaggaa gcaagcgagc
    19021 ttctcgctgc taaagacggt gccatcctat tcctaggtcg cgacacttgc ccatactgcc
    19081 gtcgcttcgc accaaaatta gctgccgtag ctgctgctca aaactggacc gttcacttct
    19141 tgcatacaca agatcccgcc tattctgcac aagaaatcgc tgcactacgt gaacaatacc
    19201 atgttccaac tgtatcaggc cttctcgttg cgaaagcaag cggcgttgac gttcgctgtg
    19261 atagcagcct aacagaagac gaaattctcg cctttgtaat ggcctaatcg tttaaaatag
    19321 taccaaaaaa gagctgagta atgactcggc tcttttcatg ttggagatga taggactatt
    19381 acgcttcttt cgattgacga tagaagataa cagctcctgc accgactact aggaatataa
    19441 caccaaggac agatgtccaa agtcctgact tctcaccagt cttaggaaga atttttcctg
    19501 ttgtagtggt tgaagctggt acttctggaa ctgttgtttc aactgtagta gttgaagccg
    19561 gtgtttcagg agtagtcgtt gtttcttctg aagttgtagt tgtagtcgtc gttgttgtag
    19621 ttgttgtgat cactttattg ataaagttca cttcttgttc tgatttatca actgtcgcaa
    19681 caagtgttgg accaccgtta tctgtaacag ttacaataac gtcatactct acttcatcat
    19741 agtcgatacc tgcatctgta ccaagtactt cttttacagt gtacttgtat gtacctggtg
    19801 catcaaatgt caattcatcg aatgtaacca caccagtgcc atcgttttta gcagttgaaa
    19861 caactgaacc gtcgtctttc ttcaagagag tgaattcaaa ctcttgatct gcaagtgcac
    19921 ggccttccaa tgttttcgtt gctttcaaaa caactttaac tggaccagct ggttttttct
    19981 tgttgacaat ttctttaaga acactcttcg taacaccatc aaattccgct ggattgatga
    20041 ccatatcttc gattggaagg taaccatctg gcgtcttgat ttctttaaga gtgtattcat
    20101 ctcttagaag attgctaatg ctaccttcac cttgatcgtc tgtagtaatt ttaccaacac
    20161 tttggcctgt acggttacgg ataacatcga actcaacatc tgccaatgga gcacctgctt
    20221 catcggtctt cttaacttta atagtaaata cataaccttc ggctttacca cctgctgcaa
    20281 ggtaagtata tcccgatgta acggtttgat caataccatt ggtagtaatc aatgttgctt
    20341 cattgctaaa ctcttctcca tcaacaggga catacggtgc ttttgtttta taaataattt
    20401 gaatcccgtc agtcggagcc aaatctggca actcaatact aaaggatttg ccgtctggtg
    20461 caaatgttag tgtcacttga tcagtaatcg tttctatatt atccagtacc caatcgccgg
    20521 ctttaaaact ccagtttccc ttctcaactc tcaatgtatc tggaatatac tctaattgct
    20581 tgctttttaa aacatcaacc accttgacat ctttcatact ttggccagaa cggttgattg
    20641 caataatgaa ccatgcatct tgtgggttct ttgaatcact ccaaccactt ttttgaatgt
    20701 ctcgtttatc tggtgttcca attccttgga attgaatttt accagcatgg ataatctttc
    20761 cttgtactgt aatatcaagt ggaatttctt ttttctgctg aataactcta tgatctacac
    20821 gaacataaaa atagaatgac ccttttatat ccgaatgttc ttcaacatac tttgtataag
    20881 tcaatttgat tttcttttct ggctctggag tgattgtcgc attcgcaaca acgttaccct
    20941 ctttgtccgt aatttggaaa tcagaacttt ggttaaatat cagttcagaa ggcaattcaa
    21001 tttctatcac atctccttct ttaaccgcat tgtttttgat atcaaaatca gcattcagac
    21061 gaaattgctg ccactgttcg acatttggtg gcgttaattc attcccatcc ttatctgtaa
    21121 agtacatttt tgtaataaca tttgtggtct cgtccccata cacatgtgaa ctcgccccca
    21181 aataaccact aaacagaatc atgactggaa gtaggatgaa agcaagtatt ttcttcatct
    21241 ttgtcataac aaactcctta attaaagtaa tttagttaac tcttttaatt tcactatctg
    21301 tgccatttta tcactattgt aacttttcgt caataattcc accatgtttt cagctaatat
    21361 atagtatttt gtaactaaat tactaaaaat atttctataa ttaagcaaat tacagactat
    21421 atattagtaa aatccttaca ataataaaaa ttgtatataa actcagctct tttcacattc
    21481 taaaccatta cttacttaac attgtttcca ttaatgataa aatgatagtt gctacactaa
    21541 tcaa
//
LOCUS       DASFSW010000019        21375 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.019, whole genome shotgun sequence.
ACCESSION   DASFSW010000019 DASFSW010000000
VERSION     DASFSW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21375
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.019"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(20..709)
                     /locus_tag="U1099_000565"
     CDS             complement(20..709)
                     /locus_tag="U1099_000565"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEM4866909.1"
                     /translation="MKFGIIAAMPQELKILVEHLQDATEIDVLGRPYYQGRIGQHEVV
                     LVQSGIGKVMSAMSVAVLADRFSVDVIVNTGSAGAVAEGIAIGDVVVANQLAYHDVDV
                     TAFGYAYGQMAGQELYYPADQVLLEQLRTVLAEQEMISHVGLIVTGDSFIAGQERIAT
                     IKTHFPEVLAVEMEGAAIAQAAVNTGKPFLVIRAMSDTAQGDANITFDEFIIQAGERS
                     AQTLIAFLEKN"
     gene            complement(718..1038)
                     /locus_tag="U1099_000566"
     CDS             complement(718..1038)
                     /locus_tag="U1099_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866910.1"
                     /translation="MGKPLLTDEILEKVKKKQYSDQSSQFGHYRPNQDISMDDALFDP
                     YLEEDYQGYEEGQTIRIPVKPTVVKSRRIETIKREAFRSKVNKILFWVIILLIMFIIA
                     VLFI"
     gene            complement(1049..1594)
                     /locus_tag="U1099_000567"
     CDS             complement(1049..1594)
                     /locus_tag="U1099_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM4866911.1"
                     /translation="MNFEEKTIERTEIFKGHIFDVVVDDVALPNGGTGKRELIFHKGA
                     VCVLAVTPEGKMILVKQYRKAIERTIYEIPAGKLELGEEDTLEDAALRELEEETGYTS
                     DKLTLLADFYSAIGFCNERIRLYLADNLIKVENPRPMDEDEVIELHEVTLEEALTLVA
                     TGDICDAKTIMAIQYLQLMRK"
     gene            complement(1603..2985)
                     /gene="glmU"
                     /locus_tag="U1099_000568"
     CDS             complement(1603..2985)
                     /gene="glmU"
                     /locus_tag="U1099_000568"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEM4866912.1"
                     /translation="MSNNYAIILAAGKGTRMKSDLPKVLHKVAGITMLEHVKRAVDAM
                     EPAKTVTIVGHKAELVQAVLEGQSEFALQSEQLGTGHAVMMAEPALAGLEGQTLVIAG
                     DTPLITGESLKNLINFHVSHKNVATILTAQTDNPFGYGRIIRNADGEVQKIVEQKDAN
                     DFEKQVKEINTGTYLFDNKRLFEALKDINTDNAQGEYYLTDVISIFRQAGEKVGAYVL
                     RDFDESLGVNDRVALATAEAVMRKRINEKHMVNGVTFINPDATYIDIDVEIGAEAVIE
                     ANVVLKGQTVIGERTVLTNGTRVRDAKIAADVVISNSDIEESVIEEGVTVGPYAHIRP
                     GSLLKKDVHVGNFVEIKASTLGQGTKSGHLTYLGNATIGNNVNVGAGTITVNYDGKNK
                     FKTTVGDNAFVGSNSTIIAPVTIGDNALLAAGSVITKDIPEDAIGIGRCRQENKEGYA
                     TRFPFHPSQK"
     gene            complement(3162..3632)
                     /locus_tag="U1099_000569"
     CDS             complement(3162..3632)
                     /locus_tag="U1099_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEM4866913.1"
                     /translation="MTPTQLWQEFLAINSQAGPEPESWAFGAEADRLADLVARGIKTS
                     TSSAHALYAVEGEEIPTAGGYDIILDGQGKAVCIIQTTKVYVTPFSQVTEKHAYKEGE
                     FRQGGDLSDIKEKSLIHWRQVHEELFTIWLAEAGLTFTDDMLVVCEEFELVYPK"
     gene            complement(3629..4180)
                     /locus_tag="U1099_000570"
     CDS             complement(3629..4180)
                     /locus_tag="U1099_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866914.1"
                     /translation="MILETNRLMLRPWSETDATDLFSQASHHEVGPAAGWPVHQSVEE
                     SREIIKTVLSQPETYALVLKETGQVIGSIGLMLGKQGGLDLPDSEAELGYWLGQGFWG
                     QGLVPEACQAILNYGFNQLNLEKVWCRAFVENSKSLRVQEKLGFVYQYLLEDVHFPLI
                     DEVRSERVNLLTREEWQSRKEKL"
     gene            complement(4193..4891)
                     /locus_tag="U1099_000571"
     CDS             complement(4193..4891)
                     /locus_tag="U1099_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEM4866915.1"
                     /translation="MTKTALITGVSSGIGLAQAEIFLENGWRVFGIDLASKPDLAGDF
                     HFLQLDLTGDLSPVFSWCQTVDVLCNTAGILDDYRPHLDIEEDELARIFAVNFFAVTR
                     LTRPYLQQMVDRQSGIIINMCSIASSLAGGGGSAYTASKHALAGFTKQLALDYAKDKV
                     QIFGIAPGAVQTGMTQKDFDPGGLADWVADQTPIGRWSQPSEIAELTLMLASGKLASM
                     QGQIITIDGGWSLK"
     gene            complement(4884..5120)
                     /locus_tag="U1099_000572"
     CDS             complement(4884..5120)
                     /locus_tag="U1099_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEM4866916.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEVHGQRLEAT
                     PYFLINVTGEGEGFSMWSPTPCDVLATDWVEVHD"
     gene            complement(5173..5967)
                     /locus_tag="U1099_000573"
     CDS             complement(5173..5967)
                     /locus_tag="U1099_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866917.1"
                     /translation="MSKQMEYRKQIEVIESQLTKENKEYMGRINGYMMIASVFHRQEE
                     AVTAQLLSIYQDVLEAQKDGLSAEDFLGKDSKQMADDLLSYLPPIGFMEVANLSGLML
                     IIYLGSQWLMDFAGTGTISLNWLGLICDAVLSLLLPSGIFLIIRGLIYQTSKIKVWAS
                     FLYIPLLFLVICGLRLWAMPKEPDLVLTGWELLVPLTLLGLALLFFQKEKLVRYVFMP
                     SYLFMIAGGVVNMVMTVPVWLNLVLLILPAMAFWIGSAILLVRKPK"
     gene            complement(5969..6286)
                     /locus_tag="U1099_000574"
     CDS             complement(5969..6286)
                     /locus_tag="U1099_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM4866918.1"
                     /translation="MKETQMLKGVLDGCVLQIISQKEIYGYELVQELRNQGFENMVGG
                     TVYPLLQKLEKNDLILSQNKPSPDGPDRKYFYLTDQGKAYLEDFWSQWTELVQKVQRL
                     KGE"
     gene            complement(6283..6447)
                     /locus_tag="U1099_000575"
     CDS             complement(6283..6447)
                     /locus_tag="U1099_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866919.1"
                     /translation="MKLDKKSRGNWKLAGITLVMAIITGWALDSILILGILLASGLMF
                     GFFTDDEEGL"
     gene            complement(6638..8650)
                     /locus_tag="U1099_000576"
     CDS             complement(6638..8650)
                     /locus_tag="U1099_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEM4866920.1"
                     /translation="MFGLTVRLALTNLKKNRRLYFPYALMTILSTAIAYIFASLAFHP
                     DLGQVKGADGVTMVLGFGMIVVMLAVAIMVFYANSFVMKQRSKEFGVYSILGLEKKHL
                     LLMTFLENLVFAASSILTGLLLGLALDKLFYALLLKVMQMPVVLASTFQLKSLVTVVI
                     YLFGIFALVSLFNIGKLGLTDSLKLVQGKKRGDKKTGFLWLQTLLALILLGAGYAIAQ
                     LVTSPMQAIPSFFGATLLVILGTYLLFHAGVISLLNWLKNRQTYYYKPDNFISVSNLI
                     FRMRKNALGLATITILSSMFLVTMVGGLNIYIGGKDYIANQNPNDYSIDVGMQPTTSK
                     AQMEKWEEWAEAILEETDIPVESKVVYPYQQAYFSEVTNQQVRFLTDEEAAGMNFSDR
                     VGIGFVLDQASYEKMTGEKLQLASDQVAIFSKGVQYQAGQTLTFDEKEMEVVQVLPKN
                     VTLGHLPDHGSFLLSQYLVIVVQDLAVFENQAENRYYMGFESSLSEEEQVDSHDTFLS
                     GSFESELWESNILPNGTNAISLAYRAYAMRGFFSFAGSLFFIGLLLSLIFLMAVVLVI
                     YFKQISEGYEDRDRFVIMTKVGLDEDQTRQSIRKQLLTVFFLPILLAFVHLAFAYKML
                     SSILLSIGVVNAGLMLQVTVGICGGYLLLYLVVYAITTRSYKQIIS"
     gene            complement(8652..9410)
                     /locus_tag="U1099_000577"
     CDS             complement(8652..9410)
                     /locus_tag="U1099_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866921.1"
                     /translation="MPLLDVQHVQKIYSSRFKAGQVEALKDIHFTVEKGEYVAIMGES
                     GSGKSTLLNILATLDKPTQGKVLLNGLDTQSIKSKEAAVFRREKLGFVFQDFNLLDTL
                     SVKDNVLLPLVLSRYPTDEMNKRLVHTLNSLGIATLQDKMPYEISGGQQQRVAVARAI
                     ITQPEILLADEPTGALDSKSTATLLDIFEKINQEGQTILMVTHSTVAASRAKRVLFIK
                     DGILYNQIYRGERTSQEMYQLISDTLTLMANRGE"
     gene            complement(9508..10500)
                     /locus_tag="U1099_000578"
     CDS             complement(9508..10500)
                     /locus_tag="U1099_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM4866922.1"
                     /translation="MNKDDFGSLVPGFLTSWLGHRLIFLIAYAVFAVAILAYSSLFDI
                     KRNLVFYAILLLSICWLLALLWDFFREFSRFGKIWRGQKVATGTASERLLLEKVERLE
                     VQNKQILEKQRQEQTELDDYYTLWAHQMKTPIAASQLLVKEVDSSPVRHQLESELFKI
                     EQYTGLVLNYLRLQSFHDDLVIEAVNLEELVRSLVKKYSLFFIQANTSLDLGQLDRLV
                     KTDKRWLGLLIEQILSNALKYCQEGTVSIFLDGDELVIRDTGIGIAESDLERVFERGF
                     SGFNGRRTQQSSGLGLYLSRKIAAELGYSLKLQSKVGQGTEVRIGIKEVELIFD"
     gene            complement(10493..11176)
                     /locus_tag="U1099_000579"
     CDS             complement(10493..11176)
                     /locus_tag="U1099_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4866923.1"
                     /translation="MQKILLIEDDKTISQLVAKNLINWGYQVQEVKDFQMVLEQMEEF
                     QPHLILLDIGLPFFNGYYWCQEIRKTSRVPIMFLSSHDQPMDIVMAINMGADDYVTKP
                     FEMTVLLAKIQGLLRRTYDFVGEQSLLWFEEISLDLKTMQVSYGQVVEELTRNEFQIL
                     RVLFEHGKEVVSREELMRELWNSDIFVDDNTLSVNIARLRKKLAELGLPDVIATKKGV
                     GYGLVWTHE"
     gene            11346..12515
                     /locus_tag="U1099_000580"
     CDS             11346..12515
                     /locus_tag="U1099_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEM4866924.1"
                     /translation="MPNLLTYIEETQYDNFYDKPINKLDILALTELSYLPFDNLVPYS
                     FTENGVRLDRLAATFEETYKNNFPPFSMVTKNRLALLGLLAKSIRFKSIKAFGFVDDY
                     QLEQEKQFSAISYRIDRKTIVTCFRGTDDTIIGWKEDFHMTYMDEIPAQRAASGYLEK
                     LMMQQGGHFYLAGHSKGGNLALYAACQQATDQQDRILAIYPFDSPGLHKKHLEAPSYQ
                     AIHQRIFPVIPQNSIVGMMLETPENVQIVQSNTLGILQHISFSWEVDGSDFKPAPALT
                     SDSLQTDQALKTWTASLTDDELRDFFDLFFGIFIKAGIERFSDITVNPLQKLQEIDRL
                     RKEIAPEEAEMVDKLIRLLFDTRYQIWRDNIPSPEISLPDWRKLFQRNTTENKEN"
     gene            complement(12530..13654)
                     /locus_tag="U1099_000581"
     CDS             complement(12530..13654)
                     /locus_tag="U1099_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEM4866925.1"
                     /translation="MKLPRFGVEEWLNVHENSAIYDIAGVSISSLTLEELFALSGTNP
                     EDFYKKLQGTKLNYGWIEGSPAFKKSVSQLYTGVKPEQILQTNGATGANLLVLYSLIE
                     PGDHVISLYPTYQQLYDIPKSLGAEVDLWQIEEENGWLPDLEKLRQLIRPTTKMICIN
                     NANNPTGAVMDRTYLEELVEIASEVGAYILSDEVYRSFSELDVPSIIEVYDKGIAVNS
                     LSKTYSLPGIRIGWVAANHQVTDILRDYRDYTMICAGVFDDLVAQLALAHYKEILERN
                     RHILEENLAILDQWIEEEPLVSYIRPAVVSTSFVKIAVDMPMEDFCLQLLQEHGVLLV
                     PGNRFERDGYVRLGFACEQETLIKGLEKLSQFLRRFDKEN"
     gene            complement(13802..14563)
                     /locus_tag="U1099_000582"
     CDS             complement(13802..14563)
                     /locus_tag="U1099_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEM4866926.1"
                     /translation="MSHQLDNAKNLYLRGIRDGEIKEVHENYMGASYTQHSTGVPDEK
                     EGFAAFFEDFFKRNPKREITIARAIEDGNFVFVHVHQKLNDGAAEWVTADIFRSDENG
                     CIVEHWDVIDAYPKTIGQTDPIYADFELTDLDKTEDNKKIVRRFLVDVLQNGKIDKFD
                     DYVAANLIQHNQEIAQGGAAYKDYLVDKAVNYDFVFKVMGQGDYVVAYSKVWIAGQDY
                     AHFDIYRLKDGKIVEHWDNKEVMPDKKDLTNLGKF"
     gene            complement(14573..16084)
                     /locus_tag="U1099_000583"
     CDS             complement(14573..16084)
                     /locus_tag="U1099_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEM4866927.1"
                     /translation="MNLFETFIERKDEWGVALFEHLRISLLALIIAIAIAVPLGLILS
                     SKKRLTEWSLQITGIFQTIPSLALLGLFIPFMGIGTLPAVVALVIYAIFPILQGTLTG
                     LGEIDPSLEEAATAFGMNKWEKLKKFKLALAMPILMSGIRTASIMIIGTATLAALVGA
                     GGLGSFILLGIDRNDSALILIGAVSSAVLAVLFGYGIRLLQDKKPKTILLALLLTLFT
                     VGASYVPMLNFSTKQLVIAGKLGAEPEILINMYKLLIEDQTDIKVEIKPNFGKTSFLY
                     EALKSGSIDIYPEYTGTITSTLLKNSSMDLSTNSDEVYTYAKEAILEQDGLVYLAPMA
                     FQNTYALAVTEDYAQKNGIEKISDLAKVQQTAVAGFSLEFNDREDGNIGLKNLYNLQL
                     NVKTMEPALRYEAIKSGNVQIIEAYSTDSKVVTYKLKILKDDKRLFPPYQAAPLLSKE
                     TLEKYPELEQALGVLAGNISTEEMTRMNYAVDVEGKSAEQVAREYLEQEGLLK"
     gene            complement(16084..16815)
                     /locus_tag="U1099_000584"
     CDS             complement(16084..16815)
                     /locus_tag="U1099_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866928.1"
                     /translation="MIDYQNVSLTCKVSGPILKNLTFDIQEGEFFVLIGPSGSGKTTT
                     LKLINRLIEQTEGEVFFQGKRLKDFDLRELRLETGYVLQQIALFPNMTVAENIALIPE
                     MKGLGKEETLTRTRELLTKVGLEPDSYLDRLPKDLSGGEKQRVSILRAIIANPKVLLM
                     DEPFSALDPISKAQLQDLIKELHEEFKMTTVFVTHDMDEAVKLADRICLMQDGQVVQL
                     GSPDELRNHPANDFVKEFIRARGGM"
     gene            complement(16808..17257)
                     /locus_tag="U1099_000585"
     CDS             complement(16808..17257)
                     /locus_tag="U1099_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM4866929.1"
                     /translation="MMEVEKRSKDYARMFDQQVGLYEDYARGHGLNAKCLSILMWIYY
                     NPGGVTQNWVSKKTYSSKQVVNATVKKFLDGGLVVLEENPADKRHKKIKLTEEGQQFA
                     SRILDPLEEAENKALSQLSQEEQELMLGLIQRYSQSLMAILKGEDDD"
     gene            complement(17568..18104)
                     /locus_tag="U1099_000586"
     CDS             complement(17568..18104)
                     /locus_tag="U1099_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM4866930.1"
                     /translation="MVKKSSSVWTSLLLGAAGGAAAAAFLASKTGKTVKEKVVNFAND
                     YKENHEEINADFVNKAQDLGKQATERFTEVKTQLETGELTVEDLVKSGKEKSLETFEQ
                     IKEKIAEQNLSTADILEAIKAKTAKAPTVDLTEEDIEDAVVVSEDIELTIDDVIIEPV
                     SETVSEVASETASETVEG"
     gene            complement(18088..18501)
                     /locus_tag="U1099_000587"
     CDS             complement(18088..18501)
                     /locus_tag="U1099_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEM4866931.1"
                     /translation="MIIEISVLIIALSIAAVAVYIVLLLKKLGTVTDEAQQTLKVLTS
                     DVNVTLYQTNELLAKTNVLVEDVNGKVSTLDPLFVAVADLSTSVSDLNASARDLTVKA
                     KSAGASTVKAGGALSALSTLSSLLGKKGDKNGKKI"
     gene            complement(18512..19288)
                     /locus_tag="U1099_000588"
     CDS             complement(18512..19288)
                     /locus_tag="U1099_000588"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEM4866932.1"
                     /translation="MDPIAIKLGPLEIRWYAICILLGLILGVYLATKEGPRKKIRQDD
                     ILDFILIAFPLSILGARIYYVAFSWSEYKDNILSIFAIWNGGIAIYGGLITGAIVLYF
                     FTQYRFINTLDFLDIVAPSVMIAQAIGRWGNFFNQEAYGKAVESLNYLPAFIRDQMYI
                     DGAYRQPTFLFESLWNLLGFGLVCVLRRRPKFLKQGEITAFYLVWYGCGRLLIEGLRT
                     DSLMFLGIRVSQWLSGVLILVGIIMVVLRRRKSSIPFYQP"
     gene            complement(19290..20222)
                     /locus_tag="U1099_000589"
     CDS             complement(19290..20222)
                     /locus_tag="U1099_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEM4866933.1"
                     /translation="MTVYVKDLVDNLRIECAYSTEELLQKQITTADITRPGLEMTGYF
                     DYYAPERIQLIGMKEWSYLMAMTAHNRYQVLSQMFQPETPVVIVARGLEIPEEMYKAA
                     KEKQIAICRSKTATSRLSGELSSYLDSRLAQRTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDDVTLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSEVQLAVYLENFETGKVFDRLGNSGDSIEIAGIAIPQIRIPVKTGRNISVVIE
                     AAAMNYRAKQMGFDATKIFEERLSNLIEHNREEV"
     gene            20437..>21375
                     /gene="manA"
                     /locus_tag="U1099_000590"
     CDS             20437..>21375
                     /gene="manA"
                     /locus_tag="U1099_000590"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEM4866934.1"
                     /translation="MMEPLFLTPVMHEKIWGGNRLRTNYHYDIPSEKTGECWAISAHP
                     NGVTTVSNGQYKGRGLDDLYKNEKHLFGNPTDDVFPLLTKILDADDWLSVQVHPDDSY
                     GLAHEGELGKTECWYILEAEDGAEIIYGHNAQSKEELRQQIEAGDWDKLLTHVPVKKG
                     DFFFVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRRDDAGNLRELHIEKSIDVLTI
                     GPVANSTPAHLKAGNLDSTLLVANPFFTVYKWNIQQEIKMEQTVPYLLVSVIEGEGAI
                     QVGETSYPLQKGSHFILPANVTDWKFTGQMNIIASHT"
BASE COUNT         6134 a         5155 c         4140 g         5946 t
ORIGIN      
        1 tattgttgtc aaaatctgct taatttttct ccagaaaggc aatcagggtc tgggcagaac
       61 gttctcctgc ttgaatgata aattcatcaa aggtgatatt ggcatctccc tgagctgtgt
      121 cactcatggc acggataacc aagaatggct tacctgtatt gacagctgct tgagcgatag
      181 ctgctccttc catctctacg gcaaggactt ctgggaaatg agttttgatc gtagcgattc
      241 tctcctgacc tgcaataaaa ctgtcacctg tcacaatcag acccacatgg ctaatcattt
      301 cctgctctgc taaaacggtt cttagctgtt ctaacaagac ttggtcggct ggataataca
      361 attcctgccc tgccatttgc ccgtaagcat agccgaaagc cgtcacatcc acatcgtggt
      421 aagccaattg attcgctaca accacatcac cgatggcaat accttcggca acagctcctg
      481 ccgaacctgt attgacaatg acatcaaccg aaaaacgatc tgccaaaaca gccacggaca
      541 tagctgacat caccttaccg atacctgact gaacaagaac aacttcatgt tgaccgattc
      601 gtccctgata gtaaggtcga cccaagacat cgatttcggt tgcatcttgt agatgctcga
      661 ccaaaatctt taattcctga ggcatggctg cgataattcc aaatttcatc tcttctccta
      721 aataaataat actgcaatga taaacattat cagtaagatg atgacccaaa aaaggatttt
      781 gtttacctta ctgcggaaag cttctcgctt gatggtttcg atacggcgac tttttactac
      841 agtaggtttg accgggattc ggatcgtttg tccctcttca tatccctgat aatcttcttc
      901 tagatatgga tcgaatagag catcatccat tgatatatcc tggtttggac gatagtgtcc
      961 aaactggcta gattggtcgg aatactgttt ctttttcact ttctctaaaa tttcatccgt
     1021 caacaagggc ttgcccattg ctagtttcct actttctcat gagttgtaaa tattggatag
     1081 ccatgatggt ctttgcatca caaatatcgc cagtagccac caaggtcaaa gcctcttcca
     1141 aggttacctc atgtaattcg ataacctcat cttcatccat tggacgagga ttttcaacct
     1201 taataaggtt atctgctaaa taaaggcgaa ttcgttcatt acagaaaccg atggctgaat
     1261 agaaatctgc caacaaggtc agtttgtcac tagtatagcc tgtttcttct tctaattcac
     1321 gcaaagcagc atcttcaagc gtatcttctt cgcctagctc caatttcccc gccggaattt
     1381 cataaatagt acgttcaata gctttgcggt attgtttgac caaaatcatt ttaccttctg
     1441 gggtaacggc taaaacgcaa actgctccct tgtggaaaat gagttcgcgc ttaccagttc
     1501 caccatttgg caaggcaaca tcatctacta ctacatcaaa aatgtgccct ttaaaaattt
     1561 ctgtccgctc aatggttttt tcttcaaaat tcatatgatt acctattttt gacttggatg
     1621 gaacggaaaa cgagtcgcat aaccttcttt gttttcttga cgacaacgac caataccgat
     1681 ggcatcctct ggaatgtctt ttgtaatgac agaacctgca gccaacaagg cgttgtcccc
     1741 aatagttact ggtgcgataa tggttgagtt agagccaaca aaggcattat ctccgacggt
     1801 tgttttaaat ttatttttac cgtcgtagtt gacagtaatg gtaccagcac caacgttaac
     1861 attattgcca atcgttgcat ttcctaggta ggtcaaatga ccagacttcg ttccttgacc
     1921 aagtgtagag gccttgattt ccacaaagtt tccaacgtga acatcttttt tcaggagact
     1981 gcctggacga atatgggcgt atggtcctac cgtcacacct tcttcaatga cagattcttc
     2041 aatatctgag ttgctgatga cgacatctgc tgcaattttc gcatcacgta cacgagtacc
     2101 attggttaaa acagtacgtt caccaatcac cgtttgccct ttcaagacaa cattagcttc
     2161 aataacagct tctgcaccaa tctcaacatc aatatcaata taggtagcat ctgggttgat
     2221 aaatgtcaca ccattgacca tgtgtttttc attgattcgt ttgcgcataa cagcttctgc
     2281 agtcgcaaga gcgacgcgat cattgacacc caaactttca tcaaaatcac gcaatacata
     2341 ggcaccaacc ttctcacctg cctgacggaa aatcgaaata acatctgtca gatagtactc
     2401 accttgggca ttgtccgtat taatatcttt aagcgcttca aaaagacgtt tattatcaaa
     2461 caagtaggtt cctgtattga tttctttcac ttgtttttca aaatcgttgg catctttttg
     2521 ctcaacaatt ttttgcactt caccatcagc attgcgaata atccgaccat atccgaatgg
     2581 gttatcagtc tgagctgtca agatagtcgc aacattcttg tggctgacat gaaagttaat
     2641 gaggttcttc aagctttctc ctgtaatcag cggtgtatct cctgcgataa caagggtctg
     2701 accttccaaa cctgcaagag ctggctcagc catcataacg gcgtggcctg tccccaactg
     2761 ttcagattga agggcaaact ctgactgacc ttcgagaact gcttggacca attctgcctt
     2821 gtgaccgaca atcgttactg ttttagcagg ttccatagca tctacagccc gtttgacatg
     2881 ctccaacatg gtaattcctg ccactttgtg taagaccttt ggcaagtcag acttcatgcg
     2941 agttccctta cccgccgcta ggataattgc atagttattg ctcatagttt tctctctttt
     3001 caatgatata ctcctattat agcaaatttt gatgtttttg gaaaatagca gtattatagg
     3061 gaattttcgt aaataaaaaa gaaactggaa tgaagccagt ttctcaaaaa tttctagtat
     3121 tagaaaacaa actacaggtt gattgcatcc aatagaattg tctatttagg ataaaccaat
     3181 tcaaattctt cacagacaac cagcatgtca tctgtgaagg tcagacctgc ttcggccagc
     3241 cagatagtga aaagctcttc atggacctgt cgccagtgga ttagactttt ctcttttata
     3301 tcagacaagt cacctccctg acgaaactcg ccctccttat aggcatgctt ttccgtcact
     3361 tgggaaaagg gcgtcacata gaccttggtg gtctggataa tgcaaacagc tttaccttgt
     3421 ccgtccagaa tgatgtcata gccaccagct gttggaattt cttctccctc tactgcgtag
     3481 agtgcatggg cagagctggt cgaggttttg atgcctctag ccaccaaatc agccagtcga
     3541 tcagcttccg ccccaaaggc ccaggactcc ggctcaggac cagcttggga attgatagca
     3601 agaaattctt gccagagttg tgtaggggtc atagtttttc cttcctagac tgccattcct
     3661 ctcttgtcag cagattcact cgttcgctac ggacctcatc aatgagtggg aaatgcacat
     3721 cttccaagag gtattggtaa acaaagccca gtttttcttg gacacgtaat gatttgctat
     3781 tttctacaaa agcacgacac cagactttct ctaaattcaa ctggttaaag ccatagttca
     3841 gtatagcttg acaagcttct ggtaccagac cctgtcccca aaatccctgt ccaagccagt
     3901 agcctagctc agcttctgaa tcaggcaaat caagaccacc ttgtttacca agcatgagcc
     3961 cgatactgcc aatcacctgt cctgtttctt tgagaacaag ggcgtaggtt tctggttggc
     4021 taaggactgt cttgataatt tctcgacttt cttccacgct ctgatgaaca ggccaacccg
     4081 ctgcaggacc aacttcatga tgactggcct gagaaaataa gtcagttgcg tctgtttcac
     4141 tccaaggtcg gagcatcaat ctattggttt caagaatcat cgaatttcct ccctacttca
     4201 aactccagcc accgtcaatg gtgataattt ggccttgcat ggaagccagt ttgccactgg
     4261 ctagcataag ggtcagctct gcgatttcac tgggctggga ccagcgtccg ataggtgtct
     4321 ggtctgccac ccaatccgcc agaccaccag ggtcaaagtc cttctgggtc atgcctgttt
     4381 ggacggcacc tggggcaatg ccgaagatct ggaccttgtc cttggcatag tccagtgcca
     4441 actgcttggt aaagcctgcc aaagcgtgct tggaggcggt ataggctgaa cctcctccac
     4501 ctgctagact ggaggcaatg gagcacatgt tgataatgat gccagactgt ctgtccacca
     4561 tttgttgcag atagggacgg gtcagtctgg tcaccgcaaa aaaattgacc gcgaagatgc
     4621 gagctaattc atcctcctca atatccaggt ggggacggta gtcgtctaga atgcctgcgg
     4681 tgttacagag gacatcaact gtctggcacc atgaaaagac gggtgataaa tcgcctgtca
     4741 ggtccagctg tagaaagtgg aaatcgcctg ccaaatcagg cttgctagcc agatcaatcc
     4801 cgaagacacg ccagccattt tccaaaaaaa tctccgcctg agctagtcca atgccgctgg
     4861 aaacgccggt gatgagggct gtcttagtca tggacttcca cccagtcggt cgcaaggaca
     4921 tcgcaggggg tcgggctcca catggagaag ccctcgccct cgccggtcac gttgatgagg
     4981 aaatagggcg tcgcctccag cctttgcccg tggacctcga tggtgtcaaa gagctggacg
     5041 tagttctccg ccccgcccca gcctgtgcga acgtactttt tcttggcctt caagcctggt
     5101 aaaatctctt caaatgtcat ggtttactcc tttattcttc tatctttcaa ccattatacc
     5161 aaaaaatcgc ttctatttcg gttttctcac caacaaaatc gcacttccaa tccaaaaggc
     5221 catagctggc agaatgagca aaaccagatt gagccaaact ggaacggtca tgaccatatt
     5281 caccacaccg ccagcaatca tgaacagata actaggcata aagacatagc ggaccaactt
     5341 ttccttttgg aaaaatagca gagcgagacc gagcaaggtg agtggtacta gaagctccca
     5401 gccagtcaag actaagtcag gttccttggg cattgcccaa agacgaaggc cacaaattac
     5461 gagaaaaaga aggggaatgt agagaaaact tgcccaaacc ttgattttgc tggtctggta
     5521 aatcagaccg cgaataatga ggaaaatccc cgaggggagg aggaggctaa gtaccgcgtc
     5581 gcagatcaag cccagccagt tgagcgaaat ggtgcctgta cctgcaaaat ccatcagcca
     5641 ttggctgccg aggtagatga tcagcatgag gccactcagg tttgcgactt ccatgaaacc
     5701 aatcggaggg aggtagctga gtaggtcgtc cgccatctgc ttgctgtctt tgcccagaaa
     5761 atcctccgca gaaagcccgt ctttttgagc ttcaagaaca tcttggtaaa tggataagag
     5821 ctgggcagtc actgcttctt cctgcctgtg aaaaacagag gcaatcatca tataaccatt
     5881 gatacggccc atgtattcct tgttttcctt ggtcagctga gattcaatca cttcaatctg
     5941 cttgcggtat tccatttgtt tgctcatctt attctccttt caatcgttga accttttgga
     6001 ccaattccgt ccactggctc caaaaatctt ctagataagc ttttccctgg tctgtcagat
     6061 agaaatactt cctgtctggt ccgtctggcg agggcttatt ctgactaaga atcaggtcat
     6121 ttttttctaa cttctgaagg agtggataaa ccgtccctcc gaccatgttt tcaaagccct
     6181 gatttctcaa ctcctgaacc aattcataac cataaatctc cttctggctg ataatttgta
     6241 atacacagcc atctaggacc cccttgagca tttgagtttc tttcataagc cctcctcatc
     6301 atctgtaaaa aatccaaaca tcaagcctga cgctaacagg atgcctaaga tcaaaataga
     6361 atccaaagcc cagccagtta taatggccat cacaagcgtg atgcctgcta gtttccaatt
     6421 tccccgactt ttcttatcca gtttcatact gcctccctac ctagtttgta atacagacta
     6481 gtttgataaa aataaaagtg attttctttg tcttctatca ctagtttgtt ttacctatta
     6541 gctttgtatc tatagtataa cacaaggcgg ctagtatgta aaacaaaata gtgaaaaata
     6601 ttcaaaaaaa aaataaaaaa cttgggttgc cccaagttta ggaaataatt tgtttgtaag
     6661 accgtgtcgt gatggcatat accactaggt aaaggaggag atagccacca cagatgccta
     6721 ccgttacttg cagcataagc cctgcattga ccacaccaat agacaacaag atagacgaga
     6781 gcatcttgta ggcaaatgca agatgaacaa aggcaagtag gataggtagg aaaaagacgg
     6841 tcagcaattg cttgcgaatg gactgtctcg tctgatcctc atccagacca accttggtca
     6901 taatgacgaa gcgatctctg tcttcatagc cttctgaaat ctgtttgaag taaatcacca
     6961 gcaccaccgc catcaaaaag atgagtgata gcaagaggcc gatgaagaaa agtgaacctg
     7021 caaaactgaa gaaaccacgc atcgcatagg cacgataggc gagactgatg gcgttcgttc
     7081 cattcggtaa tatattggac tcccaaagct cactttcaaa acttcctgat aaaaaggtgt
     7141 catggctatc tacctgctct tcttctgata gactgctttc aaatcccata taatagcgat
     7201 tttcggcttg attttcaaaa acagccaagt cttgaacaac aatgaccaga tactggctga
     7261 gaaggaagga accatggtct ggtagatgtc ctaaagtcac attcttgggc aagacttgga
     7321 caacttccat ctccttttca tcaaaggtga gggtttgacc agcttgatac tggactcctt
     7381 tgctaaaaat tgccacttgg tcagatgcca actggagttt ttctcccgtc atcttttcat
     7441 agctggcttg atcaaggaca aaaccgatcc caactcggtc agagaaattc ataccagcgg
     7501 cttcttcatc cgtcaaaaat cttacttgct gatttgtcac ctcagaaaaa taggcttgct
     7561 gataaggata gaccacctta ctttcaacag gaatatctgt ctcttctaag atagcttccg
     7621 cccactcctc ccacttctcc atttgagctt ttgaagtggt gggctgcata ccaacatcaa
     7681 tcgaatagtc atttggattt tgattggcaa tataatcttt gccaccgatg taaatattga
     7741 gtcctccaac catggtcacc aagaacatac tggagaggat ggtaatggtc gcaagcccta
     7801 gagcattttt ccgcatgcgg aaaatcaagt tggatacaga aatgaaatta tctggcttat
     7861 aataataggt ctgacggttc tttagccagt ttaagagact gataacacct gcatggaaga
     7921 gcaggtaggt cccaaggata accagaagag tcgctccgaa gaaacttgga atagcctgca
     7981 tcggactggt caccagctgg gcaatggcgt agcctgcccc caataagata agagctaaga
     8041 gggtttgtag ccagaggaag cccgttttct tgtcccctct cttcttacct tgaaccagtt
     8101 tgagtgagtc ggtcaagccc aacttcccaa tattaaaaag gctgaccaag gcaaaaatcc
     8161 caaagagata gatgactaca gttaccagac ttttcaactg gaaagttgaa gccaagacca
     8221 caggcatctg catgaccttg agcaagaggg catagaagag tttatctaga gccaagccca
     8281 gcaacaatcc tgtcaaaata ctactggctg caaaaactag attttctaga aaagtcatca
     8341 ggaggaggtg ttttttctcc aatcctagaa tactatagac accaaactct tttgaccgct
     8401 gcttcatgac aaaactattg gcatagaaga ccatgatggc aaccgccaac atgaccacaa
     8461 tcataccaaa gcccaatacc atggttaccc catcggctcc cttgacctga cctaaatcag
     8521 gatgaaaggc aagcgaggca aaaatatagg caatggctgt ggacaaaatg gtcatcaggg
     8581 catagggaaa atagagtctg cggttctttt tgagattggt cagagccaat cgaacggtta
     8641 gtccaaacat actactcacc tcgattcgcc ataagtgtca aggtatcgga aatcaactga
     8701 tacatttcct gactggttct ctcacctcgg tagatttgat tgtagaggat gccgtccttg
     8761 atgaagagaa cccgtttggc acgactagct gctactgtcg agtgggttac catcaaaatg
     8821 gtttgcccct cttggttgat tttttcaaaa atatctagaa gagttgccgt tgatttggag
     8881 tcgagggccc cagtcggctc atccgctaag agaatttctg gctgggtaat gatggcacgc
     8941 gccaccgcaa ctcgctgctg ctgaccaccc gaaatctcat agggcatctt gtcttgcaag
     9001 gtcgcaattc ccaggctatt aagcgtatga acgagtcgtt tgttcatctc atcagtcgga
     9061 tagcgagata aaaccagagg caagagcaca ttatccttga ctgagagggt gtccaagaga
     9121 ttgaaatctt ggaagacaaa gcccaatttc tcccgacgaa agactgccgc ttccttgctc
     9181 ttgatagact gggtatccaa tccgttaagc aagaccttgc cctgcgttgg cttatcaagg
     9241 gttgccaaaa tattgagcaa ggtggactta ccagaacccg actcacccat aatggcgaca
     9301 tattcaccct tttcaactgt aaaatgaata tccttcaagg cctcaacctg acccgcctta
     9361 aagcgagaac tatatatttt ttgaacatgt tgtacatcta agagtggcat cactgcaacc
     9421 tccttttctt tttctacctc cattctatcc catccgccac ccctctgcca tagccctagc
     9481 tttcatttgc gtgacagttt tgtaagatta gtcaaaaatc aactccactt ctttgatacc
     9541 gattcgcacc tctgttccct gacccacttt tgattgtagc tttagggaat agcccagctc
     9601 cgcagcaatt ttccgtgaga gatagagccc cagacccgaa gattgctggg ttctgcggcc
     9661 attgaaacct gagaaaccac gctcaaagac ccgttccaag tcgctctcag caatcccaat
     9721 tcccgtatcg cgaatgacca actcatcccc atctagaaaa atcgaaacag tcccttcctg
     9781 acaatacttg agggcattgg ataaaatttg ttctatcaag agacccagcc agcgtttgtc
     9841 ggtcttaaca agcctatcca actgccccaa gtccaagcta gtattagcct gaatgaaaaa
     9901 tagggaatat ttcttgacca gacttctgac caactcttcc aaattgacgg cttcgataac
     9961 caaatcatca tgaaaggact gtagacggag atagttcaag accaagcctg tgtattgttc
    10021 tatcttgaac aattccgatt ctaattgatg acgaactgga ctactatcta cttccttgac
    10081 caaaagttga ctggctgcaa tcggcgtttt catctggtgg gcccagaggg tgtagtagtc
    10141 atctaattct gtctgttcct gtcgctgttt ttctaggatt tgcttgttct ggacttctag
    10201 tcgctctacc ttctccagta acaaacgctc actggctgtc ccagtagcca ctttttgacc
    10261 acgccagatt tttccaaagc gggaaaattc cctgaaaaaa tcccagagga gggcgaggag
    10321 ccaacaaata ctgaggagga ggatggcata gaaaaccaag tttcttttga tgtcaaagag
    10381 gctactataa gccagaatag ccacagcaaa aacggcgtag gctatcagaa aaatcaagcg
    10441 atgaccaagc catgaggtca aaaatcctgg tactaatgag ccaaaatcat ccttattcat
    10501 gtgtccacac caggccgtag cctactcctt tcttggtcgc aatcacatct ggcagaccca
    10561 gttctgccaa tttcttacgc aggcgagcga tgttaaccga cagggtattg tcatccacaa
    10621 aaatatcact gttccagagt tctctcatca gctcctcacg gctgaccact tccttgccat
    10681 gttcaaataa aacgcgtaaa atctgaaatt catttcgcgt cagctcttcc acaacctgtc
    10741 cataggacac ctgcatggtc tttaggtcta gggaaatctc ctcaaaccag agtaaacttt
    10801 gttctccgac aaagtcatag gttcttctga gaagcccctg tattttagcc aggagaaccg
    10861 tcatttcaaa gggcttggtc acatagtcat ccgcccccat attgatcgcc ataacaatat
    10921 ccattggctg gtcatgggaa gaaagaaaca taatgggcac acgggaggtc ttgcgaattt
    10981 cctgacacca gtaataacca ttgaaaaacg gcaggccaat atccagtaaa atcaaatggg
    11041 gctgaaactc ttccatctgt tccaaaacca tctgaaaatc cttgacctct tgcacctggt
    11101 aaccccagtt tatcaagttt ttagcaacta gttggctaat cgttttatca tcctcgataa
    11161 gaagaatttt ctgcataact atctcctttc atatcactac tatttattat agcttacatt
    11221 ggaacaaatt tgagtatttt tatacattta cacaagaagt agactataaa accaggtcat
    11281 cagtggtgca gatttgtcag accaatcata tttcgttata ctagataaaa aaggaaagga
    11341 ctgctatgcc gaatttatta acctatattg aagaaactca atacgataat ttttatgata
    11401 aacctatcaa taagctagat attctggctc tgaccgaact ttcctacctc ccttttgata
    11461 atctggtccc ctactctttt acagaaaatg gtgtacgact tgatcggttg gcagcgacct
    11521 ttgaagagac ctacaaaaat aatttccctc ccttttctat ggtgaccaaa aaccgcctag
    11581 ccttactagg acttctagcc aaatccatac gcttcaaatc tatcaaggca tttggcttcg
    11641 ttgacgatta tcagttggaa caggaaaagc agttttcagc aattagttat cgcattgacc
    11701 gaaaaacgat tgtgacttgc ttcagaggga cagatgatac tattatcggt tggaaggaag
    11761 atttccacat gacctatatg gatgaaattc ctgcccaacg tgcagcgagt ggctacttag
    11821 aaaaacttat gatgcagcag gggggccatt tttatctggc cggtcattcg aaaggcggaa
    11881 atttagccct ctatgcagca tgccagcaag ctacagacca acaggataga atcctagcta
    11941 tctatccctt tgattcacct ggcttacaca aaaaacactt ggaagcacct agttatcaag
    12001 ctattcacca acggattttc cctgtcattc cccagaattc aatcgtcggt atgatgttag
    12061 aaacaccaga aaatgttcag attgtccaga gtaataccct tggcattcta caacatatca
    12121 gtttttcttg ggaggttgac ggaagcgact tcaaaccagc ccctgccctg accagcgata
    12181 gcctacaaac agaccaagcc ttaaaaactt ggacggctag tctaacagac gatgaattac
    12241 gagacttctt cgatttgttt ttcggcatat ttatcaaagc aggaattgaa cgttttagcg
    12301 acatcacggt aaatccccta cagaaattac aggaaattga tcgactgaga aaagaaatag
    12361 ctccagaaga agctgaaatg gtggacaaac tcattcggct cctctttgat acccgttatc
    12421 aaatctggag ggacaatatt ccaagccctg aaatctctct accagattgg cggaaacttt
    12481 tccaaagaaa tacaactgaa aataaagaaa actagctcaa ccacctgagc tagttttctt
    12541 tatcaaatct tcttaaaaat tgagataatt tttctaatcc tttgattaga gtttcttgtt
    12601 cacaggcaaa acctagacgg acatagccgt cacgttcaaa ccgattacca ggaactagga
    12661 gaacaccgtg ttcttgcaag agttgcaagc aaaaatcttc cataggcata tccactgcaa
    12721 tcttgacaaa gctagtggaa acaaccgctg gacggatata ggaaacgagg ggttcttcct
    12781 caatccattg gtccaaaatt gctaaatttt cttctaaaat gtgccgattt cgttctagaa
    12841 tttctttata atgggccaaa gctaattgag ccaccagatc gtcaaagaca cctgcacaaa
    12901 tcatagtgta atcccgataa tctctcagaa tgtccgtcac ttgatgattg gcggccaccc
    12961 aaccaatacg aataccaggc agggaatagg tcttggataa gctatttaca gcaattcctt
    13021 tatcatagac ttctataata gatggcacat ccagctcaga aaaggaacga tagacttcat
    13081 ccgataggat gtaggctcca acttcactag caatttctac caattcttcc aaatatgtcc
    13141 tatccatgac agcaccagtt ggattattgg cattattgat gcaaatcatc ttagtagtgg
    13201 gacgaatgag ctgacgcagt ttttccaagt ctggcaacca gccattttct tcctcaatct
    13261 gccacaaatc cacttctgct cccaaagact ttggaatatc gtagagttgt tggtaggtcg
    13321 gataaaggga aatgacatgg tctcctggtt caatcaaact ataaagaact aacaagtttg
    13381 cacctgttgc cccgttggtc tggagaattt gttctggctt gacgcctgta tagagttgac
    13441 taactgattt tttaaatgct ggtgaacctt ctatccagcc atagttgagc ttggtgccct
    13501 gcaatttttt ataaaaatcc tcaggattgg tgcctgacaa ggcaaataat tcctccaaag
    13561 tcaatgaaga aatagacact cctgctatat cataaatcgc gctattttca tggacattca
    13621 gccattcctc aacgccaaaa cgtggtaatt tcataagact tcctactttt atttatcctg
    13681 ctcttattgt accatattct ttcaacaatt gtataaatat gcgatacatc ataaaaggaa
    13741 tatttttaca attgaaaaag caggctttct caaacgagac tgcctgctct gcttttttcc
    13801 cttaaaactt ccctagattc gtcaagtctt ttttgtctgg catgacttcc ttgttgtccc
    13861 agtgctcgac aatcttaccg tctttgaggc ggtagatgtc aaagtgggcg tagtcctgac
    13921 ctgcaatcca gactttcgaa taagccacga cgtagtcgcc ctgtcccatc accttaaaga
    13981 caaagtcgta gttgacagcc ttatcgacca gataatcctt ataagctgca ccgccttgag
    14041 cgatttcctg gttgtgctga ataagattag ctgccacata gtcatcaaac ttgtcgattt
    14101 tcccattttg aaggacatcg accaagaaac gacggacgat tttcttgttg tcctcagtct
    14161 tatctaggtc agtcagctca aagtctgcgt aaatcgggtc tgtttggcca atggtttttg
    14221 gataggcatc gataacatcc caatgctcaa cgatacaacc attttcatcc gaacggaaaa
    14281 tgtctgccgt cacccactca gccgcaccgt cattgagttt ctggtggaca tggacaaaga
    14341 caaaatttcc gtcttcaatg gcacgggcaa tggtgatttc ccgcttggga ttgcgtttga
    14401 aaaagtcctc aaagaaagcc gcaaagccct ctttctcatc aggcacaccc gtactgtgct
    14461 gggtgtaact ggcacccata taattttcat gaacttcctt gatttccccg tcccgaatgc
    14521 cacgaaggta aagattttta gcgttatcta gttgatgtga catgatgttc tcctatttta
    14581 acaatccttc ttgttctaaa tattccctcg ccacttgctc tgccgacttg ccttccacat
    14641 cgaccgcata gttcatccgc gtcatttcct cggtcgagat gttgcctgcc aagaccccta
    14701 gggcttgctc caactcaggg tatttctcta gggtttcttt ggacaatagc ggagcagcct
    14761 ggtagggcgg gaagagccgt ttgtcgtctt ttaggatttt caatttgtaa gtgactacct
    14821 tgctgtctgt agaatatgct tcgataatct gcacattacc actcttaata gcttcataac
    14881 gaagggctgg ctccatggtc ttgacattga gttggagatt gtatagattt tttagaccga
    14941 tattaccatc ttcgcgatcg ttaaactcta atgagaagcc tgcaacagct gtctgctgca
    15001 cttttgccaa atccgaaatc ttttcaatcc catttttctg agcataatct tccgttacag
    15061 ccaaagcata ggtattctgg aaagccattg gtgctaggta aaccaagcca tcctgctcca
    15121 aaatcgcctc tttggcataa gtgtaaacct catcagaatt agtagataaa tccattgatg
    15181 aatttttcaa aagagtactt gtaatggtcc ctgtgtactc aggataaata tcaatagaac
    15241 ctgatttgag tgcttcatac aggaagctag tctttccgaa attaggtttt atctccacct
    15301 tgatgtctgt ctggtcctca atcaacaact tatacatatt gatgagaatt tcaggttctg
    15361 caccgagctt ccctgcaatg accaactgtt ttgtggaaaa gttaagcatg ggaacataac
    15421 tagctccaac agtaaataaa gtcaacaata gagccaataa aattgtcttg ggtttcttgt
    15481 cctgcaacag acgaatgcca taaccaaaaa gtactgcaag gacagccgac gataccgcac
    15541 caatcaaaat caaggctgaa tcattgcggt caattccaag tagaataaag gagcctaggc
    15601 ctccagcccc caccaaggct gccagagtcg ccgttccgat aatcataatg gaagccgtcc
    15661 gaatccctga catcaaaata ggcatggcaa gagctaactt aaattttttc aacttctccc
    15721 atttattcat accaaaagcg gttgctgctt cttccagaga agggtcgatt tctccaagtc
    15781 ctgtcaatgt cccttgcaag ataggaaaga tagcataaat aacaagtgct actactgctg
    15841 gcaaggttcc gattcccata aaaggaatga aaagaccaag caaagcaagg gatggaatag
    15901 tttggaagat acctgttatt tgcagactcc actcggtcaa gcgtttttta ctagatagaa
    15961 tcagtcccaa aggtactgca attgcaatcg caataatcaa agctagaagg gagatccgaa
    16021 ggtgttcaaa aagagcaact ccccactcat ccttgcgttc tataaaggtt tcaaataggt
    16081 tcattacata cctcctcgag ctctgataaa ttccttgaca aaatcattag caggatgatt
    16141 cctcaattca tcaggacttc ctaattgcac aacttgtcca tcttgcataa gacagatgcg
    16201 atccgccaat ttaaccgcct catccatatc atgggtaacg aaaaccgttg tcatcttgaa
    16261 ttcctcatgt aattccttaa tcaagtcctg caactgagcc ttactgattg gatccagagc
    16321 tgagaaaggt tcgtccatga gtaacacttt cggatttgca atgatggccc gaagaatgct
    16381 aacccgctgt ttttctcccc cagataagtc cttgggcaaa cgatccagat agctgtctgg
    16441 ctccaagcca accttggtca agagctctct cgtcctcgtc aaggtttctt ccttacccag
    16501 acctttcatt tcaggaataa gagcgatatt ttcagctacc gtcatatttg gaaaaagggc
    16561 tatctgctga agaacataac ctgtctccaa acgtaattca cgcaggtcga aatccttaag
    16621 tcgcttacct tgaaaaaaaa cctctccctc ggtctgctca attagacggt taatcaattt
    16681 caacgtagtt gtctttccgc taccactcgg tccaatcaag acaaagaact ccccttcttg
    16741 aatatcaaag gtcaaatttt tcaagattgg tccactaacc ttacaggtca gagatacatt
    16801 ttgataatca atcatcatct tctcctttta aaatagccat gagactctga ctgtagcgct
    16861 gaattaatcc tagcataagc tcctgttcct cctgactgag ttgagacagc gccttatttt
    16921 ccgcctcctc taagggatcc aaaatgcgac tagcaaattg ttgcccctcc tctgtcaatt
    16981 taattttctt atgtcgctta tctgctggat tctcctctaa aactaccagg cctccatcca
    17041 aaaatttctt tacagtagca ttgacaactt gtttgcttga ataggtcttc ttactgaccc
    17101 agttttgcgt cacacctccg ggattataat aaatccacat gagaatggat aagcattttg
    17161 cattgagccc atgtccacga gcatagtctt cataaaggcc gacttgctgg tcaaacatac
    17221 gagcataatc cttactcctt ttctcaactt ccatcatcct tccccctcct cagtgagcac
    17281 aactacctta ctaaaagcct ctggtctact agtgttcaaa acaacaacaa ttttcacaac
    17341 gacttcctcc tgtaatcatg ttagtcaata ttttttacta ttttaccaaa aaaagaaaaa
    17401 tagtcaatca ttttgactat tttgaaacat caactacctt attatagatt cccgtcttca
    17461 tccactcaaa catattaaat ccctgatatt atctgctttt aatttacaca aaacaaatga
    17521 taatcagata acaaaactag cctagaacat tctaagctag tttcatttta tccttcaact
    17581 gtttctgagg cagtttcgct tgctacttct gaaacggttt ctgatacagg ttcgatgatg
    17641 acatcatcaa ttgtaagctc aatatcttca gacacaacta cagcatcctc aatatcttct
    17701 tctgtcaaat ccactgtcgg agctttagca gtttttgcct tgatagcttc caaaatgtca
    17761 gccgttgaca agttttgttc cgcaattttt tccttgattt gctcaaaggt ttccaaggat
    17821 ttttccttac cagacttgac caaatcttct actgtcaatt caccagtttc aagttgagtt
    17881 tttacttcgg taaatcgttc agtcgcttgt ttgcctaggt cttgtgcctt attgacaaaa
    17941 tcagcattga tttcttcatg gttttcctta tagtcattag caaagtttac aactttttct
    18001 ttgactgttt taccagtttt acttgctaga aaggcagctg cagcagctcc gcctgctgcc
    18061 cctaacaaaa ggctagtcca cacgctacta gattttttta ccatttttgt ctcctttctt
    18121 acctaagaga gatgaaagag ttgacagagc tgaaagagct ccgccagctt ttacagtact
    18181 tgctccagca gacttggctt tgaccgtcaa atcacgcgct gaagcattca aatctgatac
    18241 agaagttgac aagtcagcta ccgctacaaa aagaggatca agggtcgaaa ccttgccatt
    18301 tacatcttcg actaaaacat tggttttagc caaaagctcg ttggtctgat agagagtcac
    18361 attgacatcg ctggtcaaga ccttaagcgt ttgctgggct tcatctgtca ccgtacccaa
    18421 ttttttcaac aacaaaacaa tatatactgc caccgccgcg atggacaagg caataatcaa
    18481 gacagaaatt tcaataatca tatcttctcc tttagggttg atagaatgga atagaagact
    18541 ttcttctccg caacacaacc atgataatac caacgaggat taacacccca gatagccatt
    18601 gtgaaacacg tattcccaag aacatgaggc tgtccgtccg caagccttct atcaaaaggc
    18661 gaccacaacc ataccagact aggtagaaag ctgttatttc tccctgttta aggaattttg
    18721 gccgtctacg taacacacaa accaagccaa agcctagcaa attccagaga gactcaaaca
    18781 agaaggttgg ttgacggtag gcaccatcaa tatacatctg gtctcggata aaggctggca
    18841 ggtagttcaa actttctact gctttaccat aggcctcttg gttaaagaaa tttccccaac
    18901 gaccgattgc ctgagcaatc atgaccgatg gcgctacaat atccaaaaaa tccagggtat
    18961 taataaagcg atactgggta aagaaataaa ggacgattgc acctgtaatc aaaccaccat
    19021 agatggcaat accaccgttc cagatagcga agatagataa aatgttatcc ttgtattcgc
    19081 tccatgaaaa ggctacatag taaatccgtg cccccaggat agaaagcgga aatgcgatta
    19141 gaataaaatc taaaatatca tcctgacgga ttttctttcg tggtccctct tttgtcgcca
    19201 aataaacacc tagaatcaga ccaagcaaga tacagattgc ataccaacga atttctaagg
    19261 gacctaattt tatcgcaata ggatccatat taaacctctt ctctattgtg ctcaattaaa
    19321 ttagacaagc gttcttcgaa gattttcgtt gcatcaaacc ccatctgttt ggcacggtag
    19381 ttcatggctg ctgcttcaat tacaacagaa atatttcgac cggtttttac aggaatacgt
    19441 atttgtggaa tagcaattcc tgctatttcg atactatcgc cagaatttcc caaacggtca
    19501 aataccttac ctgtttcaaa attttctaag tagacagcca actgcacttc tgatgaatct
    19561 ttcacggcac tagcaccata tagactcata atatcgataa tgccaactcc tcgaatctct
    19621 aataagtggc gcagaatttc cgcaggttca ccccagagag tcacatcgtc ttttgcatag
    19681 acatcaacac ggtcatcagc caccaatcgg tgtcctcgtt taaccaactc aagaccagtc
    19741 tcactcttac cgataccaga atcgccctga ataagcacac ccataccgta aatgtccatc
    19801 aagacaccgt gtacactcgt ccgctgagcc aggcgactat ccaagtaaga ggacaattct
    19861 cccgatagac gactggttgc tgtcttgctt cggcagatag caatttgttt ttcttttgca
    19921 gccttgtaca tctcctcagg gatttccaag ccacgtgcca ctatgacaac aggggtttct
    19981 ggctggaaca tctgagaaag aacctgatag cggttatggg cagtcatggc catcaaataa
    20041 gaccactcct tcatccctat caactggatg cgctctggag catagtaatc aaaatagcca
    20101 gtcatttcca atcctggtcg ggtaatatct gccgtcgtaa tttgcttttg caggagctct
    20161 tccgtactgt aagcacattc aatccgcaga ttatcgacca aatctttcac ataaacggtc
    20221 atttcttccc tcactttcag ttttattata ttataccata agctaggggt tgggggaaaa
    20281 acaaggggct gatttggtaa aaagaatgag tttctttctt tttctcacaa aattagcaaa
    20341 acttccacag attaaatgca cttctcttcc ttcgctacat atttttaaca aatctgttat
    20401 actttatata gatcaatact tactaataag gagaaaatca tggaaccatt gtttctcaca
    20461 cctgttatgc acgaaaaaat ttgggggggc aatcgcctaa ggaccaacta ccactacgac
    20521 attcctagtg aaaagactgg ggaatgttgg gcaatctctg cccacccaaa cggcgtaaca
    20581 actgtctcaa acggtcaata taaaggaaga ggactggatg acctctataa gaatgaaaaa
    20641 catttatttg gcaatccaac agatgatgtc tttccattat taacaaaaat ccttgatgca
    20701 gacgattggc ttagcgtcca agtccatccc gatgatagct atggcttggc tcacgaaggc
    20761 gaactgggca agacagaatg ttggtacatt ttggaagccg aagacggagc tgagatcatt
    20821 tatggtcaca atgcccaatc caaagaagaa ctccgtcagc aaatcgaagc cggagattgg
    20881 gacaagctgt tgacccatgt acccgtcaaa aaaggtgatt tcttctttgt accaagcggc
    20941 accatgcacg ccatcggtaa gggaatcctg attttagaaa ctcaacaatc tagtgataca
    21001 acctaccgtg tctatgactt tgaccgccgt gacgatgcag gcaacctccg tgaactacac
    21061 attgagaaat cgattgacgt gctgaccatc ggacctgttg ctaactccac acctgcccac
    21121 ctcaaagcag gcaatcttga ctcgacactt ctggttgcca atcccttctt taccgtatat
    21181 aaatggaata tccaacaaga aattaaaatg gaacagacag ttccttacct gcttgtcagt
    21241 gtcattgaag gggaaggtgc catccaagtc ggtgaaacca gctacccact tcaaaaagga
    21301 agccacttta tcttaccagc caatgtgaca gactggaaat ttacaggcca aatgaacatc
    21361 atcgcaagcc acaca
//
LOCUS       DASFSW010000020        21031 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.020, whole genome shotgun sequence.
ACCESSION   DASFSW010000020 DASFSW010000000
VERSION     DASFSW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21031
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.020"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(95..286)
                     /locus_tag="U1099_000591"
                     /pseudo
     CDS             complement(95..286)
                     /locus_tag="U1099_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
     gene            complement(544..1479)
                     /gene="dapA"
                     /locus_tag="U1099_000592"
     CDS             complement(544..1479)
                     /gene="dapA"
                     /locus_tag="U1099_000592"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEM4866935.1"
                     /translation="MSIQDLRDVKIITAMITPFKEDGSINFEVLPELIEHLLSHHTEG
                     ILLAGTTAESPTLTHEEELELFGAVQKIVNGRVPLIAGIGTNDTRDSIEFAKEVAAFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVEMTPETMLRLAE
                     HPNIIGVKECTSLANMAYLIEHKPEDFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMYEMFTAIEQQDIRTAAAIQRKFIPKVNALFSYPSPAPVKAVLNYLGFEVGPLRLPL
                     VPCPEEDAKRIIKVVVDGDYEATKATVTGVVRPDY"
     gene            complement(1552..2628)
                     /locus_tag="U1099_000593"
     CDS             complement(1552..2628)
                     /locus_tag="U1099_000593"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEM4866936.1"
                     /translation="MAYVVAVVGATGAVGAQMIKMLEESTLPIEKVRFLASARSAGKT
                     LQFKGQDIVIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLERIIVSTYQAVSGA
                     GMGAILETQAQLRSVLNDGVEPKAVEANILPSGGDKKHYPIGFNAIPQIDLFTENDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKGIAPIDEVKAAIASF
                     PGAVLEDDVANQIYPQAINAVGSRDTFVGRIRKDLDKENGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(2797..3891)
                     /locus_tag="U1099_000594"
     CDS             complement(2797..3891)
                     /locus_tag="U1099_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEM4866937.1"
                     /translation="MKVFASPSRYIQGKHVLFQGAEAIGKLGTKPLILCDDLVYGIVG
                     EKFLSYLVEEGMQVYRVAFNGEASDKEIQRVVEIGKEQASDVVIGLGGGKTIDSAKAI
                     ADLLGVPVVIAPTIASTDAPTSALSVIYSEEGAFERYIFYKKNPDLVLVDTAIICQAP
                     PRLLASGIADGLATWVEARAILQSNGTTMAGGGQTLAGIAIAQTCEQTLFEYGLQAMA
                     SCEAKVVTAALENIVEANTLLSGLGFESAGLAAAHAIHNGFTALEGDIHHLTHGEKVA
                     YGTLTQLFLENRPKEELEKYIRFYQALNLPTTLEELHLADAGYEDLLKVGQQATIEGE
                     TIHGMPFAISAEDVAEALMAVDYYVRSLDK"
     gene            complement(4012..4968)
                     /locus_tag="U1099_000595"
     CDS             complement(4012..4968)
                     /locus_tag="U1099_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEM4866938.1"
                     /translation="MKQFSGLSRTIQGNHLLDTCAIDILKIGHRPLVLCAKQEYNTTV
                     ESFVKVLTCHGLIVKIVYMEEWDQNSVEECGEVGFRHRADHIIGIGNSSVAACTKAVA
                     DLLDLPAVLIPTSPFTTLPWVSVENKKRERPLFNESIQLIIADREQLLTQPTSELING
                     VVNTLLYLAAIKEVKRNHLFSLVDDILIRSFESVLLRGSLQAIEDFEARILSKEVQDV
                     LEVLSTCVTLDLPSDAWDVIQQIESKLSERREDLVDEPLKRNVVLYLLLSYLTQESEE
                     FQARLKWCQQLLLSANSSNLLSLEAVLKEIALEMGEDGSTRC"
     gene            complement(5000..5668)
                     /locus_tag="U1099_000596"
     CDS             complement(5000..5668)
                     /locus_tag="U1099_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEM4866939.1"
                     /translation="MEFMLDTLNIEEIRKWAEVLPLAGVTSNPTIAKKEGDIDFFERL
                     HLIRDIIGPNASLHVQVVAKDYEGILADAKKIRELAPENVYIKVPVTPAGLAAMKTLK
                     AQGYQITATAIYTVFQGLLAIEAGADYLAPYYNRMVNLNIDSNAVIAQLSEAIDRECS
                     ESKILAASFKNVEQVNQAFANGAQAITAGADIFEAAFSMPSIEKAVNDFADDWSAIHG
                     RYTI"
     gene            complement(5683..8127)
                     /locus_tag="U1099_000597"
     CDS             complement(5683..8127)
                     /locus_tag="U1099_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEM4866940.1"
                     /translation="MKTVTEVAGTSIKTEYFGSLTDRMNKYREDVLDKKPYIDAERAV
                     LATKAYQEHREKPNVLKRAYMLKEILENMTLYIEEESLIAGNQASSNKDAPIFPEYTL
                     EFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWEKNNLRSRAGVLLPEEVQVYMET
                     GFFGMEGKMNSGDAHLAVNYQKVLEQGLRGFEERVRAAKANLDLTDPASIDKYHFYDS
                     IFIIIDAVKAYANRFVALATELAEKAPTAQRRQELLEIARICAKVPYEPAETFAEAVQ
                     SVWFIQCILQIESNGHSLSYGRFDQYMYPYVNADLEAGRETEDSIVERLTNLWIKTIT
                     INKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTV
                     RYHAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIEKGVLEEDAYDYSAIGCVET
                     AVPGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPAFGHFKDMKSFEELETAW
                     EKTLRHLTRMSVIVENAIDIALEREVPDILCSALTDDCIGRGKHLKEGGAIYDYISGL
                     QVGIANLSDSLAAIKKLVFEEGRLTPEELWHALETDYAGERGKEIQEMLIEDAPKYGN
                     DDDYADQLVTAAYDIYIDEIAKYPNTRFGRGPIGGIRYSGTSSISANVGQGRGTLATP
                     DGRNAGTPLAEGCSPSHNMDKNGPTSVLKSVAKLPTHEIVGGVLLNQKVNPQTLAKEE
                     DKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQLHPEKHRDLIVRVAGYSAFFNVL
                     SRATQDDIIGRTEHTL"
     gene            complement(8279..9580)
                     /locus_tag="U1099_000598"
     CDS             complement(8279..9580)
                     /locus_tag="U1099_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM4866941.1"
                     /translation="MSKIDTQKIIVPIMKFVNMKGIIALKDGMLAILPLTVVGSIFLI
                     LGQLPFEGLNAAIASIFGAEWTEPFMQVYSGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     AGVLSLSSFFILLKSSYIPVDGEPIGDAIAKVWFGGQGIIGAIIIGLVVGAIYTTFIQ
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYIVSKMATNGGTFIEMIYDVIQV
                     PLQGLTGSLPGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKALLAADKLS
                     LENGAHIVTQQFLDSFLIMSGSGITFGIVFAMLFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFVLVPILAAFIVYGAISIGFMQPFSGVTLPWSTPAIISGFM
                     VGGWQGAVVQIIILAVSTIVYFPFFKIQDKIAYESETKEPV"
     gene            complement(9593..9898)
                     /locus_tag="U1099_000599"
     CDS             complement(9593..9898)
                     /locus_tag="U1099_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM4866942.1"
                     /translation="MVKIALFCAAGFSTGMLVNNMKIAAADAGVEVEIDAHSQGKIAD
                     YLDEIDVALLGPQVAYTLDKSKALCDEKQVPIAVIPMADYGMLDGKKVLQLALSMVS"
     gene            complement(9931..10254)
                     /locus_tag="U1099_000600"
     CDS             complement(9931..10254)
                     /locus_tag="U1099_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM4866943.1"
                     /translation="MEMIVADQLIMGLIIYAGDAKSHAYQALSLVKAGEEERAEEELE
                     LADAALLEAHNMQTKFLAQEAGGTKSDITALFVHSQDHLMTSITEINLIKEIIDLRKE
                     LKKVG"
     gene            complement(10442..11416)
                     /locus_tag="U1099_000601"
     CDS             complement(10442..11416)
                     /locus_tag="U1099_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEM4866944.1"
                     /translation="MKQEKRRQLAKIAYLYYEEGKSQAEIAAETGIYRTTVSRMLTKA
                     KSEGIVKIEIQDFDRRLYRLEKYVQEKYGLKGLELVENSTDEDQLDLENRLAQAAADM
                     LTNLIDDGKKVGFSWGRSLSLIVDKIGNHRMKGVKFVPIAGGPSHIHARYHVNTLIYD
                     MANKFRGECSFINATIIQENQELAQGILSSKYFEELRADWKDLDVAAIGIGGRVDEKN
                     RQWLDMLTTADFQALSDEGAVGETCCRGLNQYGQAIVEDLQKRTIAISLDDLKEVPDT
                     IALAYGEEKAQAILAVLRAGYIHHLVTDERTIVKVLELDNDVHVHTNL"
     gene            complement(11429..12172)
                     /locus_tag="U1099_000602"
     CDS             complement(11429..12172)
                     /locus_tag="U1099_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM4866945.1"
                     /translation="MERLDKIIQLVSQHEKIDVNSLAEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNIRMSFRYDVKKRIAKEAANLIADNETIMIESGSTCALLAE
                     EICKTKRNVTIITNSYFIANYVRQYDSCQIILLGGEFQKDSQVTVGPLLHKMIQFFHV
                     DKAFVGTDGYDSEHGFTGKNLMRSEVVQYMSDVAENMIVLTDSSKFTKRGTVRRFGLS
                     QVAQVITDTSLSEDLVKELENARVTVRLV"
     gene            12304..13080
                     /locus_tag="U1099_000603"
     CDS             12304..13080
                     /locus_tag="U1099_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEM4866946.1"
                     /translation="MNTRKGIIFNIQHFSLHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KRKPEPMLDAVSKKMTIMGEEKSVDDIISEVMKDIDFYEESGGGLTLSGGEIFAQYEF
                     AKAILMEAKRHGLHTAIETTAFVEHHKFVDLIQYVDFIYTDLKHYNTISHRKVTGVNN
                     NLIIQNIQYAFSIGKEIVLRIPVIPDFNNSLEDAEQFACLFNQLKIDKVQLLPFHQFG
                     ENKYKLLNRTYAMTDVKALHPEDLKDYQAIFIKHNINCYF"
     gene            complement(13377..13664)
                     /locus_tag="U1099_000604"
     CDS             complement(13377..13664)
                     /locus_tag="U1099_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEM4866947.1"
                     /translation="MAKVYEFEAVIHPVPDKGGAYIIFPYDIREEFGKGRVKVHARFD
                     GHPYDGSIVNMGVKDEAGNICYIIGVQKATRLAIGKQAGDRVQVTIQERME"
     gene            complement(13675..14328)
                     /locus_tag="U1099_000605"
     CDS             complement(13675..14328)
                     /locus_tag="U1099_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEM4866948.1"
                     /translation="MKINELEKRLLAVADASQAQPMKAYMKNHFDFLGVRTPDRRKVA
                     RQFFKESKAQGIDWEFVEACWSKPYREFQYIAIDYLVTKKKDLVLADLPRLKKLAQSK
                     SWWDSIDGLDKLVGKIVLDNPEAKQTILEWSLDDDFWLRRIAIDHQLLQKEKMDTELL
                     EKILVNNLNQIEFFINKAIGWSLRDYSKTNPDWVRAFLKKYSSQMAGLSIREASKYI"
     gene            complement(14318..14827)
                     /locus_tag="U1099_000606"
     CDS             complement(14318..14827)
                     /locus_tag="U1099_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEM4866949.1"
                     /translation="MIDIRSARIEDAADLVAIYAPYVEKTAITFETEVPTVEAFASRI
                     EKTLEKFPYLVSVEEGKVVGYAYASTYYARAAYDWTVELSVYIKQEARGKGIGSLLYD
                     AIEEDLKARGFKNFLACIALPNPASLSLHKKRGYEQVAHFKNVGYKFDTWHDIVWLQK
                     SLVGKQNED"
     gene            complement(14828..15445)
                     /locus_tag="U1099_000607"
     CDS             complement(14828..15445)
                     /locus_tag="U1099_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HEM4866950.1"
                     /translation="MKLQKKIMLSVVLGLSLVSLSACQSIANWWKNTKEEWIGLEMTV
                     RTYDENSQLIDEMSGRSLSISRNQEFDSVDAEGYSNADSSVLKVTLGNYEIDHVGSSL
                     IAAEEGLEDLYAKYQTTVDIANYDRAIPLVNRMVSSLKNDFTGKAKVVLIRSQNGTPL
                     ATYVGDKVSLYASDAPKTSELLIDGKRLIIYRCDYTIYDRELLEN"
     gene            15603..16454
                     /locus_tag="U1099_000608"
     CDS             15603..16454
                     /locus_tag="U1099_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEM4866951.1"
                     /translation="MKQFCAKHKMKLLIFLVVWSFFSGCKVLLTFFGKPDGMDGKYPL
                     FFLTISLVALYVFPMILIIRYIAKRFDISKKVIHLSWILGITASFYFSGLGQTLLGAF
                     WLFIVKPPQTFIQNWGAAVTAPFHEEFGKGLVVLLVLLLLKKLTLKNAVVSGMVVGLS
                     FQIIEDGLYVFQQIFKSKADGFATLIERMLHAGGTHWAFSLAFAVGLVALVSKNSGMS
                     KKQGLFWMLMAVLAHFFLNTPFNEGLTSNSGEITVLMLCFSFCVALAAFFKVDQIETN
                     QHTSNTY"
     gene            16467..16625
                     /locus_tag="U1099_000609"
     CDS             16467..16625
                     /locus_tag="U1099_000609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866952.1"
                     /translation="MTGFCYVTNQVFEMVVDSSISIISLYPIPSVQEIADNYKKPPIT
                     DGHSQSMK"
     gene            complement(16701..18012)
                     /locus_tag="U1099_000610"
                     /pseudo
     CDS             complement(16701..18012)
                     /locus_tag="U1099_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
     gene            18229..19038
                     /locus_tag="U1099_000611"
     CDS             18229..19038
                     /locus_tag="U1099_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEM4866953.1"
                     /translation="MKEKFQMCKTYLPVVLATIGFVNGCKIIFGELGKPNGMEAKYPL
                     FLLTISLVAIYILPLLVLTYSLAKRYNISKQVIGLSWLLGLTSSVSFSELGHTAIGYF
                     LLEIVKASNDFLNDWGAAISGPLAEEIGKGLTVLLVLLICRKMTLKNALVSGVIVGLG
                     FQIMEDITFVFRDMFMNKLDGFETILERVGQAGWAHWVFTLLFAIGLVALLTKNTGMS
                     KAQGVFWIGASFGIHFLFNSPFNTGIFQTVFPILSISLGLLAYQTVEKLSE"
     gene            complement(19082..20752)
                     /locus_tag="U1099_000612"
     CDS             complement(19082..20752)
                     /locus_tag="U1099_000612"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEM4866954.1"
                     /translation="MKTDIEIAQSVTLKPITEIVEKVGISFDDIELYGKYKAKLSFDK
                     INAVKDNAPGKLILVTAINPTPAGEGKSTVTIGLADALSKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGFAQVLPMEDINLHFTGDMHAITTANNALSALIDNHIHQGNAVGIDQRR
                     IIWKRVVDLNDRALRKVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDINDLKER
                     LANIVIGYRFDRSPVYVRDLAVEGALTLILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALRLADYTVTEAGFGADLGAEKFLDIKVPNLPKAPDAVVIVATHRAL
                     KMHGGVAKTELSAENVEAVKAGFSNLKRHVENIQKYGIPAVVAINEFVSDTADEIAAL
                     KELCAEIGVPVELASVWANGADGGVELAETVVATIENQAANYQRLYKAEDSLEEKVTK
                     IVTQIYGGSGVVFEKKARNQLTEFAKNGWDKLPVCMAKTQYSFSDDQFALGAPTDFDI
                     TVREFVPKLGAGFIVALTGDVMTMPGLPKAPAALNMDVAADGTAIGLF"
BASE COUNT         6068 a         4827 c         3837 g         6299 t
ORIGIN      
        1 gcttgttttc tccataagac cagttcctcc cttatttggt atctacgtaa taaaaaaact
       61 tccctaacca ttgctgatta gagaaggata tttcttaaaa aatgagacca taaaggacag
      121 ccgcaagcaa gcctccgatg attggaccaa ctactggtac ccaagcatag ccccaatctg
      181 aatctccctt atgcttcaat ggaaggatgg caagcatgat acgaggacca aggtcacgcg
      241 cagggtttag ggcatatcct gtcggaccac caagagaaac tccgagtcaa aacaaaggtt
      301 ccgattgctt cgctaattgt aaatgtcccc cagacattgt tcgctctctt atttcaaggc
      361 tcacgttaaa atacttcgct tttttatttt caagctcaga taagaatagt ccactggact
      421 attcttatcc cctaactatc ctcgctttcc agtttccagg ctcgggtctt ctatggtcac
      481 tttcatattt cttggtgacc agctgaaaca gttccccgaa ctgtttcact cccccagaca
      541 tttttaataa tccggtctta cgactcctgt cacagtggct ttagttgctt cgtagtcgcc
      601 gtcaacgact actttgataa tgcgtttggc atcttcttct gggcatggaa ccaacggcaa
      661 gcggagtgga ccaacttcaa atccaaggta attaagaacc gccttaactg gtgcaggact
      721 tggatatgag aagagggcat tgactttcgg aatgaacttg cgttgaatag cggctgctgt
      781 tcgaatatct tgttgttcga tggctgtaaa catttcatac atctcatcac cattcgtgtg
      841 agatgcaaca gaaatcacac catctgctcc caagttcatt gcatggaaag catcgccatc
      901 ctcacctgta taaatcaaga agtcttctgg tttatgctca attaggtaag ccatattggc
      961 aaggcttgta cactctttaa caccgatgat atttggatgc tctgccaaac gcaacatggt
     1021 ttctggtgtc atttcgacca caacacgccc tggaatattg tagataatga ttggtaaatc
     1081 agaagcatct gcaatggcct tgaagtgctg atacattccc tcttgagagg gtttgttgta
     1141 gtaaggcacg atagcaagcc ctgccgcaaa gccgccgaat gccgccactt ctttggcaaa
     1201 ctcaatcgag tcgcgcgtat cgtttgtacc gatacctgca attaggggaa cacgaccatt
     1261 gacaattttt tgcacagcac caaatagttc cagttcttcc tcgtgtgtca gggttggact
     1321 ttcagcagtt gttcctgcta ataaaatccc ttctgtgtga tgggacaaca gatgctcaat
     1381 caactcaggt aaaacttcaa aattaatcga gccatcttct ttgaaagggg taatcattgc
     1441 tgtaataatt tttacatcac gtaaatcttg aatagacata ggcttcctct cttactgatt
     1501 gttgctaaaa aaggctgagt gcagcctttt gtgtactttt ggttaataaa attatttcaa
     1561 ttcaaacttc aattctgccg ttggacgaac tagaccacgc tcatgaaggg tttcagcgat
     1621 ttgaacagag ttccatgcag cgcctttgag caagttgtca gaaacaaccc acatgtgaat
     1681 accgttttcc ttgtccaaat ctttacggat gcgaccaaca aaagtatcgc gactaccaac
     1741 agcattaatg gcttgcggat aaatttgatt agccacatcg tcttctaaaa cagcccctgg
     1801 gaaggatgca atagctgcct tcacttcatc aatcggtgca attcccttag tttcaatgta
     1861 aacagattca gagtgagctg acaagactgg aatacggacg caggttgcag aaacggcaat
     1921 gctgtcatct tccatgattt tcttagtctc ttttgtcatc ttcatctctt cgtatgtgta
     1981 gtcattttca gtgaagaggt cgatttgcgg aatagcattg aaaccgattg gatagtgctt
     2041 cttatcgcca ccagaaggaa gaatattcgc ttctaccgct ttgggttcca cgccatcatt
     2101 caagacagaa cgaagctgag cttgtgtttc caagatagct cccatacctg ctccagagac
     2161 tgcctgatag gtggatacga tgatccgctc caagccccat ttctgacgaa caggctccaa
     2221 ggcaaccatc atttgaatgg ttgagcagtt tggacaagca atgataccat tgtgggcatc
     2281 aagcgcgtga gcatttacct caggaacaac cagaggaaca tctggatttt gacggaagta
     2341 agaggtattg tcaactacta cagctcctgc ttttacagcg taaggagcgt acttggcaga
     2401 tgtagagcca ccagctgaga agagggcaat atccactcct tcaaaggctg tttctgttgt
     2461 ttcttcaatg acaatatcct gccccttaaa ctgcaaggtc ttacctgctg aacgggcaga
     2521 agcaaggaaa cgcactttct cgattggaag tgttgactct tccaacattt taatcatttg
     2581 ggcaccaact gcaccagtgg caccaacaac agctactaca taggccatag aaatctcctt
     2641 taagtcaatt cttcaaaatt ttctaaatat tttatattta acaattatat cataattttt
     2701 tcaaatgaaa agccctgaga aagaatttcc caaggcaatt acttcgaacc tctattcacc
     2761 agtttagatg gacatgctca actgtgaatg ctcttcttat ttatctaatg agcgaacata
     2821 gtagtcaact gccatcagag cttctgcaac gtcttctgct gaaatagcaa atggcatacc
     2881 gtgaattgtt tctccttcaa tcgtcgcttg ttggccgact ttcaatagat cttcataacc
     2941 agcatcagcc aaatgtaact cctccaaagt tgttggaaga ttcaaggctt gatagaaacg
     3001 gatgtatttt tccaattctt ctttcggacg gttttccaag aagagctggg tcaaggtccc
     3061 ataagccact ttttcaccat gtgtcaaatg gtgaatgtct ccctccaagg cggtgaatcc
     3121 attgtgtatc gcatgcgccg cagccaaacc tgcactttca aatcctaaac cacttagcaa
     3181 ggtattggct tcaacaatat tctctaaagc agctgtcaca accttggctt cacagctggc
     3241 catggcttga agtccgtatt caaagagtgt ttgttcacag gtttgtgcaa tagcgatacc
     3301 agcaagtgtc tgacctcctc ctgccattgt tgttccattg ctttgtaaaa tagcacgcgc
     3361 ttctacccac gtcgccaagc catctgcaat accagaagcc aaaagacgtg gcggtgcttg
     3421 acaaataatc gctgtatcta ccaagacaag atctggattt ttcttataga aaatatagcg
     3481 ttcaaacgcc ccttcttcag aataaatgac agataaagca gatgtcggag catctgttga
     3541 agcaattgtt ggagcaatga caactggcac tcctagtaaa tcagcaatcg ccttggctga
     3601 gtcaatcgtc ttaccaccac ctagaccgat tacgacgtca ctagcctgtt cttttccgat
     3661 ttctacgacg cgctggattt ccttatcaga tgcttctcca ttaaaggcaa cacgatagac
     3721 ttgcatccct tcttcaacga gataggacaa aaacttctca ccgacaatcc catatactaa
     3781 atcatcacaa agaatcaaag gctttgttcc caattttccg atagcttctg caccttggaa
     3841 aaggacatgt ttaccttgaa tatagcgaga tggacttgca aatactttca taagaattcc
     3901 tccttgaaat aatagatatt gttatattga gcaataaacg acagttagca ttgccgaaag
     3961 taagaggtat gagcctgcat tttaaatatt gctattgtac agcgatgcct actagcaacg
     4021 tgtcgagcca tcttctccca tctctagtgc aatttccttt aaaactgcct ccagcgacaa
     4081 tagattgcta gagttggcag acaaaagcaa ctgttgacac cacttcagcc tggcttgaaa
     4141 ttcttctgat tcctgtgtca aataggacag aagcaagtag aggacaacat ttcgtttcaa
     4201 tggctcatcc accaaatcct ctcttctttc gctgagcttt gattctattt gttgaatcac
     4261 gtcccaagca tcactaggca ggtctaatgt tacacaagta gaaagaactt ctaagacatc
     4321 ttgtacttct tttgataata tccgagcttc aaaatcctct atagcttgaa ggctacctct
     4381 taataaaacg ctttcaaaac ttctgataag aatatcatct accaagctga atagatgatt
     4441 tctttttact tcttttatgg ctgctagata aagaagagta ttgaccactc catttatcaa
     4501 ctccgaggta ggttgtgtca ataattgctc cctatccgct ataattagct ggatggactc
     4561 attaaacaat ggtcgctctc ttttcttatt ttccactgat acccaaggta gagtggtaaa
     4621 cggtgaagtc ggtataagaa cagctggcaa gtctagtaaa tctgcaacag cctttgtaca
     4681 agctgctaca gaactattac caatgccaat aatatggtct gcacgatgtc ggaaaccaac
     4741 ttccccacat tcttcaaccg agttctggtc ccactcctcc atatagacta ttttaacaat
     4801 aagtccatga caagttaaaa ctttcacaaa actttctaca gtcgtattat actcctgttt
     4861 tgcacataaa acaagggggc gatgacctat tttcaaaatg tcaatcgcac aagtgtctaa
     4921 aagatgattt ccttggatag tgcgtgaaag acctgagaat tgtttcatag tgccccctta
     4981 ttttataata tagagatgtt tagatagtat accttccatg aatggcagac caatcatctg
     5041 caaaatcatt taccgccttt tcaatggatg gcatagaaaa ggctgcttca aatatatctg
     5101 cacctgccgt aatagcctgc gccccatttg caaatgcttg gtttacttgc tccacatttt
     5161 tgaatgacgc cgctaaaatt ttactttccg aacactccct atcaatcgct tctgataact
     5221 gagcgatgac cgcgttcgaa tcaatattca gattgaccat gcgattgtag tacggcgcta
     5281 agtaatctgc acccgcttca atagctaaca agccctgaaa aacggtataa atcgctgttg
     5341 ctgtaatctg ataaccttgc gctttcaatg ttttcatagc agccaaacca gcaggcgtta
     5401 ccggaacctt aatatagaca ttttccggtg ccaattcacg aattttttta gcgtctgcca
     5461 aaatcccttc ataatccttt gccacaactt gcacatgtag tgaagcattt ggaccgatga
     5521 tgtcacggat aaggtgcaaa cgttcaaaaa agtcaatgtc accttctttc ttcgcaatcg
     5581 ttgggtttga ggtcaccccc gcaaggggta gaacctcagc ccattttcgt atttcctcta
     5641 tatttaaggt gtccagcata aattccataa taataacctc ttttacaatg tatgttctgt
     5701 acgaccaatg atgtcgtctt gtgttgctct cgacaataca ttgaagaatg ctgaataacc
     5761 tgctacacgg acaatcaaat cacggtgttt ttcaggatgt agttgtgcat caatcaaggt
     5821 ttctcgagag acaacattgt actgaatatg atagccatgc aagcggttaa agaatgtccg
     5881 caagagggca attaattttt gtttgtcttc ttctttggca agtgtttgtg gattgacctt
     5941 ctgattgagc agaacgcctc caacaatttc atgtgttggt aatttggcaa ctgatttcaa
     6001 aacggatgtc ggtccatttt tatccatatt atgtgatgga gaacaacctt ctgccaacgg
     6061 tgtacctgca tttcgaccat ctggagttgc caaggtacca cgcccctgtc caacgttggc
     6121 tgaaatggat gatgtacctg aataacggat accaccaatc ggaccacgac caaagcgagt
     6181 gtttggatat ttggcaatct catcgatata aatatcatac gctgctgtta ccaattggtc
     6241 tgcataatca tcatcgttac catattttgg tgcgtcctca atcaacattt cttggatttc
     6301 cttaccacgc tcaccagcat agtctgtttc caaagcatgc cataattctt ctggtgttaa
     6361 tcgtccttct tcaaagacta attttttgat tgctgctaga gaatcagata aattggcaat
     6421 gccaacctgc agaccagaaa tgtaatcgta aatcgctcca ccttctttca agtgcttacc
     6481 acgaccgata cagtcatctg tcagagctga gcagagaatg tctggtactt ctctttctaa
     6541 agcaatgtca attgcatttt caacgatgac actcatccgt gttagatggc gaagcgtctt
     6601 ttcccatgcc gtttcaagct cctcaaagga tttcatatct ttgaagtgac caaaggctgg
     6661 tgcaaaccgt ttgcctgatg caggatcgat accatcattc atggtaatca ataaaacttt
     6721 cgggaagttc atatagctca tacctgtaca acggtatccc catttaccag gaacagccgt
     6781 ttctacacag ccaatcgcac tgtagtcata ggcatcttct tccaagacac ctttctcaat
     6841 aaaggatgga atgataattt catcgttgtt aaaggcaggc ataccaaaac caagtttcat
     6901 gacttcaata cattcattca tgaaacgagc atctaggcct gcatgatagc gaacagtcaa
     6961 gtttggctgt ggcaggtgtg tttgggcaac agatttcaat actaagtaag aaagtggatt
     7021 gacagcatcc ttcttatcac gtgtttgtcc accaattgtt acattttggt atagtggact
     7081 accagcagat gagaaggtat gtgattgact acgaaccttg ttgatagtaa tggtcttaat
     7141 ccacaagtta gtcaagcgtt cgacgatgct gtcttccgtt tcacgtcctg cttctaaatc
     7201 agcattcaca tacggataca tgtattggtc gaaacgtcca taggatagag aatgtccatt
     7261 cgactcgatt tgcaagatac actggataaa ccagacagat tgcactgcct ctgcaaatgt
     7321 ttcagcaggt tcatacggca ccttcgcaca aatccgtgca atctccagta gttcttgacg
     7381 gcgttgagct gttggtgctt tctctgccaa ttcagtagcc aaggcgacaa aacgatttgc
     7441 gtatgctttc accgcatcaa tgatgataaa gattgagtca tagaaatgat atttatcaat
     7501 acttgcagga tccgtcaaat cgagattagc ctttgctgca cgaactcgct cctcaaaacc
     7561 acgcaaacct tgttccaata ccttttggta attgacagcc aagtgggcat cgccagagtt
     7621 catcttacct tccataccaa agaagccagt ttccatataa acttgaactt cttctggaag
     7681 caagacacct gcacgtgaac gaagattatt tttttcccag aatggagcga tgctgcgcaa
     7741 ctgctccttg gtttcctcgg taatatagaa gacgtcccca tcccgttttt cgaataaatc
     7801 caattcattc aatacaaact ccaaggtata ttctgggaag atgggagcat ctttatttga
     7861 agaagcttgg ttacctgcaa tcagactttc ttcttcaata taaagcgtca tgttctccaa
     7921 tatttccttg agcatatagg ccctttttaa tacattgggc ttctctctat gttcttgata
     7981 ggcttttgtt gctaaaacag cccgttccgc gtcaatatag ggcttcttat caaggacatc
     8041 ttctctgtat ttgttcatcc gatctgtcag acttccaaaa tactcagttt taatgctagt
     8101 gccagctact tcagtcactg ttttcatagt tttacccttt ctttcgaaac ttaattttgt
     8161 ttcgtttgta aattaagtat atcctgtaat aggggttatt gtcaattgat gctagtgagc
     8221 gctaagattt ccgaagccgg ctgcacattt gtgcaactct tttgtctgac atcattgact
     8281 aaactggttc tttcgtttca ctttcatagg caatcttatc ttggattttg aagaatggga
     8341 aataaacaat agttgagaca gctaaaataa tgatttgaac aactgcacct tgccaaccac
     8401 ccaccataaa accagaaatg attgctggag tagaccatgg tagggtcaca cctgaaaatg
     8461 gttgcataaa gcctattgag attgcaccgt agacaatgaa agccgctaag atcggtacca
     8521 aaacaaatgg taagaacatt acaggattca tcacaattgg aaaaccaaag acgactggtt
     8581 catttacgtt aaagagtgca ggaaaggccg ctaccttacc aagagcctta tattgttttg
     8641 atttagcggc aaatagcatt gcaaaaacaa taccgaaggt aatacctgaa cctgacataa
     8701 tcaagaaact atctaagaat tgttgggtca cgatgtgggc gccattttcc agagacaatt
     8761 tatctgctgc taaaagggcc ttattggcat cgaggtttga caatagtaaa gcagtaacga
     8821 caccattgac aactgactga ccatgtacac caaaccacca gaggaaggaa atgaagaaag
     8881 caattccaat cgcacctggt aatgaacctg tcaaaccttg tagtggcact tgaatcacat
     8941 cgtaaatcat ttcaatgaat gtaccaccgt ttgtcgccat cttggaaaca atatagacaa
     9001 tcatagatag caaaaagatg acaaaagctg gaatcatagc ttcaaactgc ttggcaatag
     9061 cttgcggtac ttgttcaggc atcttgataa caatatgacg ctgaataaaa gtcgtataga
     9121 tagcgcccac taccaaacca ataataatcg caccgataat tccttgacca ccaaaccata
     9181 cttttgcgat ggcatcaccg attggttctc catcaactgg aatataagat gatttcaaga
     9241 ggataaagaa tgaagacaag gacagtacgc ccgcagggag aggctcaact ccactatttt
     9301 ttgcgtaaga ataaccaatc gcaaaacaag aaatcaagcc cataatcgca aaggttccgc
     9361 tatagacttg cataaacggt tcagtccatt ccgctccaaa tatagaagct attgcagcat
     9421 ttaagccttc aaaaggcaac tgccccaaaa ttaagaaaat actaccaact actgttaaag
     9481 gtaagatagc caacatacca tcttttagag cgatgatccc tttcatattc acaaatttca
     9541 tgattgggac aataattttt tgtgtatcaa ttttggacat gaccatactc ctttatgaaa
     9601 ccatgctgag tgccaattga agaacttttt tcccgtccaa catcccgtag tctgccattg
     9661 gaatcacggc aatagggact tgcttttcat cacagagcgc ttttgactta tcaagcgtat
     9721 atgctacttg tggtcccagt aaagctacat cgatttcgtc aaggtaatct gcaattttcc
     9781 cttgggaatg cgcgtcgatt tctacttcga cacctgcgtc tgctgctgcg atcttcatgt
     9841 tgtttaccaa catgcctgta gaaaaaccag cggcacaaaa tagagcgatt ttaaccattt
     9901 cggtttcctc ctaaatataa tttttttgtt ttagccaact ttttttagtt ctttccgcaa
     9961 atcaatgatt tctttaatca aattgatctc tgtaatagat gtcatcaaat gatcttgcga
    10021 atggacaaat agtgctgtaa tatcagattt tgttccccca gcttcttgtg ctaagaattt
    10081 tgtctgcata ttgtgagctt ctaataatgc tgcatctgcc aattctaatt cctcttctgc
    10141 tcgctcttct tcacctgctt tgacaagcga caaagcttgg taggcgtgac ttttcgcatc
    10201 tccagcataa ataatcaatc ccatgatcaa ttgatctgca acaatcattt ccatttcttt
    10261 ttcctccgat gtgttttatt tgtaagttat tatatttctt tcgtaagtaa atcatatcat
    10321 tttctcttta tagcgtaaat atatttatag aacattttct gttttaattt atttctgcac
    10381 ttttgtgcat ttttggggat aaaaaatact tcaaccacct ttaagcgatt gaagtactct
    10441 cttacaaatt tgtatggaca tgcacatcat tatccagttc taaaactttt acgatggttc
    10501 gttcatctgt taccagatga tgaatgtagc cagctcgcaa tacagctaaa atggcttgag
    10561 ccttttcctc cccatatgca agagctatgg tatctggaac ttcttttaaa tcatctaaag
    10621 agatggcaat ggtcctcttt tgcaaatcct caacgatagc ttgaccgtat tgattcagac
    10681 cacgacaaca ggtttcaccg actgcacctt catctgataa ggcttgaaag tcagccgtcg
    10741 tcaacatatc taaccattga cgattttttt catcgactcg accgccaata cctattgctg
    10801 ctacatctaa gtccttccag tcagcacgta gctcctcgaa atatttagaa gaaagaatac
    10861 cttgagcaag ttcttgattc tcctggataa tcgtcgcatt gataaaacta cattctccac
    10921 gaaatttatt ggccatatcg taaatcagag tattcacatg ataacgtgcg tggatatgac
    10981 ttggaccgcc agctattgga acaaatttga cacctttcat acgatgattc cctatcttat
    11041 ctacaataag actgaggctt ctcccccatg aaaaaccaac tttctttcca tcatcaatga
    11101 gattggtaag catatcggct gcagcctgtg ccagacgatt ttctaaatca agctggtcct
    11161 catcggtgct gttttcaacc agttctaaac cttttaatcc atatttttct tgaacatatt
    11221 tttctaatcg atataggcgc ctatcaaaat cttgaatttc tatttttaca atgccttctg
    11281 acttagcctt ggtcaacatt ctactgactg tcgttctata aattcctgtc tcagcagcaa
    11341 tctcagcttg gcttttcccc tcttcgtagt aaagataggc tatttttgcc aactgccttc
    11401 ttttttcttg cttcatactg cctccaacct aaactagcct tactgtgact ctagcatttt
    11461 ctaattcctt gacaagatct tcagaaagcg acgtatctgt aatgacctga gcaacttgac
    11521 ttaaaccaaa tctgcgtact gtccctcgtt ttgtaaattt actagaatct gttaacacaa
    11581 tcatgttttc agcaacgtca gacatgtact gtactacttc gcttcgcatt aagtttttac
    11641 ctgtaaaacc atgctcacta tcatagccgt cagttcctac aaaagcctta tcaacatgga
    11701 aaaactgaat cattttatgc aagagcggcc caactgttac ttgggaatct ttttgaaact
    11761 caccacccaa taaaataatc tgacagctat catattgtcg cacataattg gctataaaat
    11821 aagaatttgt tatgatggtc acatttctct tggttttgca aatttcttct gccaagagag
    11881 cacaagttga cccagactct atcataatcg tttcattgtc ggcgataaga tttgctgctt
    11941 cttttgcaat tcgttttttt acgtcatagc gaaaggacat ccgaatatta aggtcatcgc
    12001 cactatttag aacagcatag ccgtgttctc tacgtaataa accttttgat tctaacttat
    12061 ctaaatcttt acggatggtt accttagaaa cgtccagttg ctccgccaaa ctattgacat
    12121 ctattttttc atgttgtgat accagttgta taattttatc taatcgttcc atttcatcac
    12181 ctcatcatta ttctatcaaa ttttgactct ttttcaacta tttgcaacct ttcaacttac
    12241 gttcgtaatt gtaaaacttt cttttgtgtg ataaaatcaa attaatcgaa aaggagaaag
    12301 agcatgaata cacgaaaagg cattattttt aacattcaac atttttcgct ccatgacggt
    12361 cctggtatac gtacgacggt tttcttgaaa ggttgccccc tacgttgtcc ttggtgtgca
    12421 aatccagaat ctcaaaaacg aaaacctgaa cccatgcttg atgctgttag taaaaaaatg
    12481 accatcatgg gagaagaaaa atctgttgat gacatcatat ccgaagtcat gaaagacatt
    12541 gatttttatg aagaatctgg tggtgggctc acgctgtctg gcggagaaat ttttgcccaa
    12601 tatgagtttg cgaaagctat tcttatggag gcaaaaagac acggccttca taccgctatt
    12661 gaaaccaccg ctttcgtaga acaccacaaa tttgttgacc ttatccaata cgttgacttt
    12721 atctatactg atttaaaaca ttacaataca atttctcata gaaaagtcac cggcgtcaac
    12781 aataacctta tcatacaaaa tatccaatac gccttttcca tcgggaaaga aatcgtccta
    12841 cgtatccctg ttatccctga ctttaataat tccctcgaag atgctgaaca atttgcctgt
    12901 ctctttaatc aactcaagat tgacaaagtt caactactcc ccttccatca atttggagaa
    12961 aataagtata agcttctcaa tcgaacctat gctatgaccg atgtcaaggc tctccatcca
    13021 gaagatttaa aagactatca agcaatcttc atcaaacata atatcaactg ttatttctaa
    13081 ctttgagaaa tacaaaaact tcctcggctg agccaatagc attcctatag aaatcacata
    13141 ctgaaatatg cttctgatag agtatctgaa gcatacttag tatataaaaa aggcccacca
    13201 taaaggtggg ccagggcatc gtttccgatg agaatagaag gttcacacaa ctattcaagg
    13261 tccgctcctg cgtttcaatt aaatgaagac ctccctagcg tcgaaagtga caaagtcact
    13321 actcggctca tcgcatgaat ctattctacc ataatctcaa ggatttgcaa gcctgattac
    13381 tccattcgtt cttgaatggt tacttgtact ctatcaccag cctgttttcc aatagccagt
    13441 cgagtagcct tttgaacgcc aataatataa cagatattgc ctgcttcatc cttaactccc
    13501 atattgacaa tggagccatc atagggatga ccatcaaatc tggcatgtac ctttaccctc
    13561 cccttgccaa actcttctcg tatatcatag ggaaagatga tataggctcc gcccttatcc
    13621 ggtactggat gaatgaccgc ttcaaattca tatacctttg ccataacagc cctcctagat
    13681 atatttacta gcttcacgaa tggataagcc tgccatttgg gaactgtact tttttagaaa
    13741 agcccgtacc cagtcaggat tggtcttgga ataatctcgc aaactccagc caatcgcctt
    13801 gttaatgaaa aattcaatct gattgagatt gttgaccaaa atcttctcaa gaagttccgt
    13861 atccatcttt tctttttgga gaagctggtg gtcaatggca atccgcctca accagaaatc
    13921 atcatccaaa ctccattcca agattgtctg cttggcctct ggattgtcca aaacaatctt
    13981 accgactaat ttatccaagc cgtcgatact atcccaccaa gacttacttt gagccagttt
    14041 tttcaaacgg ggtaaatcag ctagaaccaa gtcttttttc ttggtaacca gataatcaat
    14101 ggcgatatat tgaaactcac ggtagggctt agaccagcaa gcctctacaa attcccagtc
    14161 aattccctga gccttagatt ctttgaaaaa ttgcctagca acttttcggc ggtcaggagt
    14221 tcgcacacct agaaagtcaa agtgattctt catataggct ttcatgggtt gagcttgact
    14281 ggcatccgcc acagccaaca aacgtttttc taattcgtta atcttcattt tgcttaccta
    14341 caagagattt ttgaagccag acaatatcat gccaggtatc aaatttataa ccaacatttt
    14401 tgaaatgtgc tacctgttca taccctctct tcttatgaag agaaagcgag gctggattag
    14461 gaagagcaat acaggctaaa aagttcttaa agccacgcgc tttcaaatct tcctctatgg
    14521 catcatataa aagacttccg attccttttc cacgtgcttc ttgcttgata tatacggata
    14581 attccaccgt ccaatcgtaa gccgcacggg catagtaggt tgaagcgtag gcataaccca
    14641 caactttacc ttcttctact gagaccagat aaggaaactt ctccaaggtc ttttcaattc
    14701 gacttgcaaa ggcttcgaca gtcggtactt ctgtttcaaa ggtaatagcg gtcttttcta
    14761 cataaggagc ataaattgcc acaaggtctg ccgcatcctc tatccttgcg gaacgaatgt
    14821 ctatcattta gttctccaat aattctctat cataaattgt ataatcacaa cggtagataa
    14881 tcaaccgctt gccatcaatt aagagttcag aagtcttagg tgcatcagat gcataaagag
    14941 acactttatc acctacatat gtagcaagcg gggttccgtt ttgcgaacga atcaaaacca
    15001 cctttgcctt acctgtaaaa tcatttttga gactagatac catacgatta acaagcggaa
    15061 ttgcacgatc gtaatttgca atatctactg tggtctgata tttcgcatac aagtcttcca
    15121 aaccttcttc tgctgcaatc aaagatgagc ctacgtggtc aatttcgtaa ttcccaagtg
    15181 tcaccttcaa gacagacgaa tccgcattgg aatagccttc ggcatccacc gagtcaaatt
    15241 cctgattccg cgaaatcgaa agggatctac cagacatttc atctatcaat tgagaatttt
    15301 catcgtatgt ccttaccgtc atctccaaac caatccattc ttccttggta tttttccacc
    15361 aattcgcaat ggactgacaa gcagacaaac taacaaggct caaaccaaga acaacagata
    15421 acatgatttt cttttgcaat ttcatataat agtcctccaa catattttac ttattatatc
    15481 acggaataca aaaaccgaga aaaaatagat agacctgaga taataaaaag atggcaatga
    15541 taaactatag cacggtaggg ataactgaca tctactaaat taatataaag gaagtcattt
    15601 ttatgaaaca attctgtgct aaacacaaaa tgaagctact tatttttcta gtcgtttgga
    15661 gttttttctc tggctgtaaa gttcttctta ccttttttgg gaaaccagat ggaatggacg
    15721 gaaagtatcc tctcttcttt ctaactattt ccttagttgc actttatgtc ttccctatga
    15781 ttcttatcat tcgttatata gcgaaacgtt ttgatatttc taaaaaagtt atccatctca
    15841 gttggatttt gggtataaca gctagtttct acttctctgg tcttggtcaa acgcttttag
    15901 gagcattttg gttattcatc gtcaaaccac ctcaaacatt tatccaaaac tggggagcag
    15961 ctgtcaccgc tcccttccat gaagagtttg gtaagggcct agttgtacta ctcgtactcc
    16021 tattgctaaa aaaacttact ctaaaaaatg ccgtagtcag cggtatggtt gtaggtttga
    16081 gtttccaaat tattgaagac ggtctctatg tcttccaaca aatctttaaa tctaaagcag
    16141 atggcttcgc cactcttatc gaacgtatgc ttcatgctgg tggaactcac tgggccttct
    16201 cccttgcatt cgcagtcggt ttggtcgctc tcgtctcaaa aaatagtggc atgtcgaaaa
    16261 aacaaggact tttctggatg ctgatggctg tcttagcaca ctttttcttg aatactccat
    16321 tcaacgaagg actaacatct aactctggtg aaataaccgt tctcatgctc tgctttagtt
    16381 tctgcgtggc acttgctgca tttttcaaag tagaccaaat tgaaacgaat cagcacacca
    16441 gtaacacata ctgaaacaag cctgcattga caggcttttg ttatgttaca aaccaagtat
    16501 tcgaaatggt tgttgattca tcaatatcta ttatcagtct ttatcccatt ccatcggtcc
    16561 aagaaatcgc agataattat aaaaaaccgc ccattacgga cggtcatagt cagtcaatga
    16621 aataaggtcg ctcgatgatc ttgacactag tctaggaaac gcagccacaa ctaatacaag
    16681 aagcttatct ccctagagta ttaaaaatta aagacgtcat tcaaattctc agccatagtc
    16741 gggtgagtga aaatctgtgt tttgaagtag ctataaggaa tcttattgtc aatcgccata
    16801 gcaatcaagt taatcagctc ctctgaattg cgaccaaaga gagtcgcccc cagaacaagc
    16861 ttactttcct tatccacgat aaccttgaaa atgcctttga ggtcattatt gacatgggca
    16921 cgtggcatat tggcgacagg caattcgttg gcaatgtagt cgtaacctgc ttccttggct
    16981 tccttctcgg tcagaccgac acgagacagc acaggcgtaa taaagacgct ggttggaata
    17041 gacttacgtt ggctcaagct ataagttcca gtcccagtca acttgccgaa aacgatacgg
    17101 aagtcgtcta gggatgtgta agtgaattgt ggtccgccat tgacatctcc caccgcatag
    17161 acacctggaa cagtcgtttc gcagtagtca tccaccttaa cagcaccatt ttccaaaagc
    17221 tcaatcgccg tattttccaa gcccaagtca gcagtgttcg gcacccgacc tgtcgcatag
    17281 aggacggcgt cgaagattgc cgtttcgcca ttaaccgtca cagcaacttg ctcgcccgca
    17341 gctgctactt gctcgagttt cgcacccaag acaaaggtca cgccagcttc ttccatatac
    17401 tccttggcta acttagcaac cacttcctct tctctcggta aaatagcaga gctgacttca
    17461 tataccgtca cctggctgcc gagtttgctg taaagcccag caaattccaa tccaatgttg
    17521 cctccaccga taattgccaa cgtgttagga cgagtttcca agttctgaat gcctgtgctg
    17581 tcataaacgt gagctgtatc caacaagcct ggaattggaa gcacgcgcga cttagcacct
    17641 gtattgatga cgatggtttc cgcagttagt tgaatggatt cctcaccagc cgatacttcc
    17701 acaaccttgt ctgcaacaaa acgtgcgtgt ccttgataca aactagcacc actgcctttc
    17761 aagactgcct cgtttttgtt tcgcagacga gtggtgactg tttctttttg atccattacc
    17821 tgctcaaaag ttcagttttt atctgctgct accaagaggg tcttggttgg aatacagcca
    17881 atattgatac aagtcccgcc aaacatagca gtattttcct caaccaaggc aacctttttc
    17941 ccagctactg gcagcttacc tgctaaggtc tttacatcca aaaccaatga ccaataaatc
    18001 atattgttcc ataacatact ccttttattt tttaaacctt agtctagcaa aaaagaaaat
    18061 ttgagacaca attccgttac ggctctatta tggagtccta gttgcaggaa aaaacattac
    18121 aataaccgat aaggtttaga taaaaaatag gcgaattata gacaaaatgt actcattttt
    18181 gatacaatgt atccatacac cagtttatta atacagaaag gtcaaactat gaaagaaaaa
    18241 tttcaaatgt gtaaaaccta tcttccagtc gtcttagcaa cgattggatt tgtcaacggt
    18301 tgcaaaatta tctttggaga attaggtaag ccaaatggca tggaagctaa atatcctctc
    18361 tttctcctaa ccatttcctt ggtcgctatt tacatcctcc ccctactagt actgacatat
    18421 tccttagcta aacgctataa catctccaaa caagttatag gtcttagctg gcttttaggc
    18481 ttaacaagta gcgtctcttt ttctgaactg ggacatacag ctatcggata tttcctgctc
    18541 gaaattgtta aagctagtaa tgatttccta aatgactggg gtgcagctat ttcaggtcca
    18601 ttggcagaag aaatcggtaa agggctaact gttctacttg tactgcttat ttgtagaaaa
    18661 atgacactaa aaaatgcatt ggtaagcgga gtgattgttg gattaggttt ccaaatcatg
    18721 gaagacatca cctttgtttt tagagacatg ttcatgaata aattagacgg ctttgaaacc
    18781 attcttgaac gcgttggtca agctggttgg gcccattggg ttttcacgct tctctttgcc
    18841 attggcttag tagcccttct cacgaaaaat acaggtatgt caaaagcaca gggagtattt
    18901 tggatcggtg caagctttgg aatccatttt ctctttaatt caccattcaa cactggtatc
    18961 ttccagacgg tgtttcctat cttgagtatt tcccttggct tacttgccta tcaaacggtt
    19021 gagaaactat ctgaatagac aaaaaatccg cctttgagcg gattttttat attcattctt
    19081 gttagaacaa gccgatagct gtcccgtctg ctgccacatc catgttgaga gctgcgggag
    19141 cttttggcaa accaggcatg gtcatgacat ctccagtcaa ggcaacgata aagcctgcac
    19201 ccaattttgg cacaaattca cggactgtaa tgtcaaagtc tgtcggcgct ccaagggcaa
    19261 actgatcatc tgagaagctg tactgggtct tggccatgca gactggcaac ttgtcccaac
    19321 cattcttagc aaattcagtc agctggttac gagctttctt ctcgaaaacc acacctgagc
    19381 caccataaat ctgcgtaaca atcttggtca ctttctcttc caagctatcc tcagccttgt
    19441 aaagacgttg gtagtttgct gcttgatttt caatagtagc aacaactgtc tcagccaatt
    19501 caacaccacc atcagcgcca tttgcccaaa cacttgccaa ctcgacaggt acgccaattt
    19561 cagcgcaaag ttccttcaac gcggcaattt cgtcagctgt gtcagataca aattcattga
    19621 tagcaacgac tgctggaata ccgtatttct gaatattttc aacgtgacgc ttcaagttag
    19681 aaaaaccagc tttcactgcc tctacatttt cagcagaaag ttctgtcttg gctacaccac
    19741 cgtgcatttt aagcgcacgg tgggtagcaa caatgactac tgcatccgga gccttaggca
    19801 agttcggcac cttgatatct aggaatttct ctgctccaag gtctgcacca aaacctgctt
    19861 ctgttacggt atagtcggcc aaacgcagag ctgttgtagt tgcaagaact gagttacagc
    19921 catgagcaat attagcaaat ggacctccgt ggacaaaggc tggcgtgcca tagatggttt
    19981 ggacaaggtt tggtttaata gcgtccttca aaataagagt caaagcacct tccaccgcca
    20041 aatcacgcac gtagacagga ctgcgatcga aacgataacc gataacaata tttgccaaac
    20101 gttctttcaa gtcattgatg tctgtcgcca agcataaaat cgccatgatt tcagaagcta
    20161 cggtaatatc gaatccatct tcacgcggaa taccattgag cggaccaccc aatccaacag
    20221 ttactttacg caaagcacgg tcattaaggt ccacgacccg tttccagatg atcctgcgtt
    20281 ggtcaatgcc tacagcattt ccttgatgga tatggttgtc aatcaaggct gaaagagcgt
    20341 tgttagctgt ggtaatggca tgcatatcgc ctgtaaaatg caggttgata tcttccatgg
    20401 gcaagacttg tgcaaaacca ccaccagcag ctccgccctt gattcccata acaggcccca
    20461 aagaaggctc acggagggca atcatagttt tctttccaat tttagacaaa gcatccgcca
    20521 gaccaatggt tactgttgac ttaccttcac cagcaggcgt tgggttgata gctgtcacca
    20581 agatcaattt ccctggcgca ttgtctttca ccgcattgat tttatcaaag gacaattttg
    20641 ccttgtattt tccatagagt tcaatatcgt caaagctgat accaactttt tcaacgattt
    20701 ctgtgattgg tttcaaggtt acactttgtg caatttcaat atctgttttc atgagagctc
    20761 ctaatttcct tattttttat ggacattata ccataccaca gcttattttc gcatattttt
    20821 tactatttat gcactaacgt acggaatttt cagaaaaact acaggtttct ttacttaaac
    20881 agatacaaat ttttaaaacc tgcattctca tcatagctct acacttccat ctaagactgg
    20941 gtttagttag ttttttcaaa agagagtcag tattctttta aaaattgcct tgatgagcaa
    21001 gcaaacgacc gcttgcataa agcacttcac t
//
LOCUS       DASFSW010000021        20747 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.021, whole genome shotgun sequence.
ACCESSION   DASFSW010000021 DASFSW010000000
VERSION     DASFSW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20747
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.021"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(48..251)
                     /locus_tag="U1099_000613"
     CDS             complement(48..251)
                     /locus_tag="U1099_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM4866955.1"
                     /translation="MVQGTVKWFNAEKGFGFIAQENGPDVFAHFSEIQSNSFKSLEEG
                     QKVTFEVEQGQRGLQATNITKIG"
     gene            complement(411..1244)
                     /locus_tag="U1099_000614"
     CDS             complement(411..1244)
                     /locus_tag="U1099_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM4866956.1"
                     /translation="MSQQKLIGYHPGTGFIHSLSAVSKLLFFLIVSILAMITYDTRLI
                     LFIAVFSLALFKMSGIRYKEISLVLILTIIFAAMNALMVHLFAPRYGVELYGADTPLL
                     SGLGVYSLTSQQAFYLVNLLLKYFCTVPLAIIFLMTTHPSQFASSLNQIGVSYKVAYA
                     VSLTMRYIPDIQEEFYTIRMSQEARGLELSKKGKLMDRIKGNLSLVIPLIFSSLERID
                     TISTAMELRRFGKNKKRTWYTQQPLQRIDYAVLLFILALVVVTIYLFFINQGRFYNPW
                     R"
     gene            complement(1241..2914)
                     /locus_tag="U1099_000615"
     CDS             complement(1241..2914)
                     /locus_tag="U1099_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866957.1"
                     /translation="MKKIIEFKDFTFKYQAQQEPTLKDIQLTIYQGEKVLIIGPSGSG
                     KSTIGQCLNGIIPNIYKGEHSGSLLLDGQEAFNLSVYDKSLLVSTVLQDTDGQFIGLS
                     VAEDLAFALENDMESQVVMKQKVGDWAERLSLTDLLAHRPQDLSGGQKQRVSLGGVLI
                     DESPILLFDEPLANLDPKSGQDTIDLIDRLHKQEGTTTIIIEHRLEDVLYRQVDRVVL
                     INDGRILFNGGLDDLLRTSLLQENGIREPLYLSTLRALGYPIAQAEHLSSVWEIDIEA
                     LNIASLPTLHFENGEKESLLEVKHLHFSYPNKQVLTDIQLTIHKGERLAIVGKNGAGK
                     STLAKVLCGFIQGEGEILWKGQSIKEDSVKERAERIGYVLQNPNQMISQTMIFDEVAL
                     GLRVRGVTEEEIEHRVHHILKVCGLYPFRNWPISALSYGQKKRVTIASILVLNPEIIL
                     LDEPTAGQDQRHYREMMEFLDQLNAQGHTIVMITHDMQLMLDYSDRAVVVVDGQIIED
                     ASPAEILSDDMVIERANLKETSIFHLAERLGVNPLELTTFYMQEERRGR"
     gene            complement(3021..3344)
                     /locus_tag="U1099_000616"
     CDS             complement(3021..3344)
                     /locus_tag="U1099_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEM4866958.1"
                     /translation="MAGRFYEAIGFAVATFGDIKSVTLPEGGNLILMQADSFAQRVPF
                     QVVASGNEVLISLNVASRKEVDTLIERVEANSGQITGCPTDARGFYGASFTDLDGHHF
                     NVIVM"
     gene            complement(3601..4584)
                     /locus_tag="U1099_000617"
     CDS             complement(3601..4584)
                     /locus_tag="U1099_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEM4866959.1"
                     /translation="MSKDIRVLLYYKYVPIENAKEYAAEHLAFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDYVHANPLFSDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDIDPLVTTGAYLSPKEFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVRGELWDGKMYVFDERIAVDVNHVNPVVVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEHKYVRGCSAECRAHERNRYISENGLTRQEWAERLEAIGETLTPANA"
     gene            complement(4795..5715)
                     /locus_tag="U1099_000618"
     CDS             complement(4795..5715)
                     /locus_tag="U1099_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866960.1"
                     /translation="MVWTEEKLRPIIHSILNPDEEVLSLYQSKTTKSIYGCILWNSYY
                     YKVFRVSNHPSQSTYKQPTFYDFHGEFYMKGAIRTYLYHTNSWYELSKQSYYLLLFFQ
                     KLWDEKQEVEASWQNGRMQIRLVVEEEGWQVHYRFPDNQAREVERLLASGMLVAIRSS
                     RNFIKIRTSRFVAPYIALIKQRQLYRKKPSKAMLQWEKYQSQLIEIQRTYRLVEENAP
                     KTFFGHWLNKVQLYLCSAIASYWEEVIKGVEWQEAREIKDQRKIEKPHNPIEDKWKEN
                     IARRHKRKVDQLIGHDTLEKLRQLKTELDD"
     gene            complement(5823..6368)
                     /locus_tag="U1099_000619"
     CDS             complement(5823..6368)
                     /locus_tag="U1099_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEM4866961.1"
                     /translation="MKNNSIKTVVATGIGAALFVVIGHLINIPTFVPNTSIQLQYAVQ
                     SLLAVVFGPVVGFLVGFIGHTLKDSLTYGPWWSWILASGVFGLVVGLTKKRLRIQEGI
                     FEGKDILFFNLVQIAANVLAWGVIAPVLDILIYSEAANKVFAQGLVAGIANSITVAIA
                     GTLLLAVYAKSQTKTGSLSKD"
     gene            complement(6389..7237)
                     /locus_tag="U1099_000620"
     CDS             complement(6389..7237)
                     /locus_tag="U1099_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEM4866962.1"
                     /translation="MSNNLLVLQSDFGLVDGAVSAMIGVALQESRDLVVHHLTHDITP
                     YNIFEGSYRLFQTVEYWPEGTTFVSVVDPGVGSKRKSVVALTEQNHYIVTPDNGTLSF
                     IKKYVGIKAVREISEVANRRANTEHSYTFHGRDVYAYTGAKLASGHISFEEVGPELSV
                     DKIVEIPTVPTEVGSDYVKGAIDILDVRFGSLWTSITREEFYTLQPQFEDRFEVTIYN
                     NDMLVYQNQVTYGKSFADVRIGQPLIYINSLYRVGVAINQGSFAKAYNVGVGQNWHIE
                     IKRISN"
     gene            complement(7669..8385)
                     /locus_tag="U1099_000621"
     CDS             complement(7669..8385)
                     /locus_tag="U1099_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM4866963.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPETN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRSAFGKNGGNMGASGSVSFMFDKKGVVVFAGDDADAIFELLLEADVEVDD
                     VEAEDGTITVYTAPTDLHKAIVALKESGIQEFNVTELEMIPQSEVSLEGDDLATFEKL
                     YDALEDDEDVQKIYTNVDGF"
     gene            8546..8857
                     /locus_tag="U1099_000622"
     CDS             8546..8857
                     /locus_tag="U1099_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM4866964.1"
                     /translation="MKRNKHLPLTETTYYILLALLEPAHGYHIMQKVEDMSDGDVKIA
                     AGTMYGAIENLLKLKWICSVPSSDKRRKVYQVTATGQEIIKLETQRIQKLHHIAKELG
                     F"
     gene            8868..9494
                     /locus_tag="U1099_000623"
     CDS             8868..9494
                     /locus_tag="U1099_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEM4866965.1"
                     /translation="MIRHKLFTSLQEEEHWINSIQSEGYQLVKVTPWTAAYHFEKCSR
                     TPHPVRLDFHEHIAKGEYSNYLSLFEDCGWQPIQGSRRCGIHYFQQTIEASSTEIFSD
                     TESKKAFYSRYQNYAYSYFGLFLTLFFIHYQVGLQNGYTLWNPKSWYLTPELWERTGT
                     SFWFGFLFETPFALFRSGIIPLFFLAWSIYFLHIAEKGKKEAKKLEKY"
     gene            complement(9566..11734)
                     /locus_tag="U1099_000624"
     CDS             complement(9566..11734)
                     /locus_tag="U1099_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM4866966.1"
                     /translation="MKKFLSFEFWQKFGKCLMVVIAVMPAAGLMVSIGNSIPLISPES
                     ELLIRIGNIIAQIGWGIIGNLHLLFALAIGGSWAKEKAGGAFSAGLAFILINLITGHF
                     FGVTTDMLADAAATVTTVFGTEIPVSGYFVNILGQPALNMGVFVGIIAGFVGATTYNK
                     YYNYRKLPDVLTFFNGKRFVPFVVIYRSVLVALGLAIFWPVVQTGINSFGKWIASSQD
                     TAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTSLGGTYDILTGAQAGAQVFGQDPLW
                     LAWITDLINLKDAGDMAQYNDLLANITPARFKVGQMIGSSGILMGLTYAMYRNVDEDK
                     KHKYKGMFISSALAVFLTGVTEPIEFMFMFAAMPLYVVYAFVQGAAFAMADVVNLRMH
                     SFGNIEFLTRTPMAIKAGIGMDVINFIWVTALFAVGMYFIANFMIKKFNLATAGRNGN
                     YDTETTDVVSNSNVDTADANSQVVQIINLLGGRDNIADVDACMTRLRVSVKDVAQVGD
                     ENAWKQAGAMGLIIKDSGVQAVYGPKADVLKSDIQDLLESGVAIPRTEIVATEEVAPE
                     TQFKGVTETVYSVAEGQAIAITEVKDPVFSQKMMGDGYAVEPSSGNVYAPVSGIVTSV
                     FPTKHAVGILSDKGVEVLVHVGLDTVALNGAPFSAKVTDGQRVEAGDLLLVADLDAIR
                     SAGRETTIVVAFTNTAEIKSVSLGNLGQVSQDSQVATVEL"
     gene            complement(11768..12559)
                     /locus_tag="U1099_000625"
     CDS             complement(11768..12559)
                     /locus_tag="U1099_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEM4866967.1"
                     /translation="MKLLTINVHAWLEERQDEKLDVLAKTIAEKAYDVIALQEVNQLI
                     TADLVTKDLKADNYGLILLEKLRALGQFDYSYYWSNSHIGYDKYEEGIAFLTKLPVYE
                     VDPFYCSQNKSVDSILSRKIMGLTVFYQDQLIDLYSCHINLPGSEEEDQLDNVRTIVE
                     RTGNRRFKILMGDFNTDAFSDREAYEAITELGLYDSYNLAMEKDQGITVEKAIDGWAG
                     HSQEKRLDYIFLNQKKEVLSSLVIFNGENRPIISDHFGVEVEINV"
     gene            12758..13465
                     /locus_tag="U1099_000626"
     CDS             12758..13465
                     /locus_tag="U1099_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEM4866968.1"
                     /translation="MSKYKKVYQDIKKKIEEQVWSTGQALPTENELMEVYSYSKDTIR
                     KALSLLEMDGYIQKKQGKSSIVIEHGLMKDQYLSEIKTAGELNKRSQHQIQTQLTSLY
                     IIQGQEDLMSTFEVDDKVDFYRVSRVRTIDGERLEYDISYFDRRVVPYLSKEIAESSI
                     YRYLEEELHLTISHSRREISFRFANEEEKQLMDLGDYDMVVVVTSITYLSNGQAFQYG
                     TISYRPDKVSFVSMAKR"
     gene            complement(13692..15956)
                     /gene="glgP"
                     /locus_tag="U1099_000627"
     CDS             complement(13692..15956)
                     /gene="glgP"
                     /locus_tag="U1099_000627"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEM4866969.1"
                     /translation="MTKFTTFAETNTSKKLADLTNEEIYLQLLNYVKDAAASKPKNTG
                     KRKVYYISAEFLIGKLLSNNLINLGVYKDVQAELAAAGKSLAQVEDVEPEPSLGNGGL
                     GRLASCFVDSMSTLAINGEGVGLNYHCGLFRQVFKKNEQEAEPNFWIENDSWLIPTTI
                     SYDVPFKNFTLKSKLDRLDILGYKKETKNYLNLFDIESVNYDLITDGISFDKTDIKEN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAVERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGIEFAEAVAIVKNMTGYTNHTILAEALEKWPLEFLEEVVPH
                     LVDIIKELDALIRAEIKDPAVQIIDESGRVHMAHMDIHFSNSVNGVAALHTEILKNSE
                     LKAFYELYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLLAFA
                     DDKEVHAKLAEIKFNNKLALKRYLKDNKGIELDENSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKNGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMDNIYTFGKDSDTIIDLYDKAGYVSADYYNGDANIKRAVDFIVSDEVKALGNEE
                     RLGRLHHELISKDWFMTLIDLAEYIEVKEQVFADYEDQDSWNKKVVHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
     gene            complement(15949..17457)
                     /gene="malQ"
                     /locus_tag="U1099_000628"
     CDS             complement(15949..17457)
                     /gene="malQ"
                     /locus_tag="U1099_000628"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019312918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEM4866970.1"
                     /translation="MTNRTSGILMHITSLPGKFGIGTFGQSAYDFVDFLVETKQTYWQ
                     ILPLTTTSYGDSPYQSFSAIAGNTHLIDFDLLVEDGLLATEDFQDVNFGDNPEKVDYA
                     LIYQVRRPILEKAVQAFLQDDKKKAAFLEFEKANSSWLTDYAEFMAIKEYFGNKALQE
                     WEDKKVVARNEEALDKYRLELANQIDYYKVTQYFFFSQWKQLKEYANKHHIKIIGDMP
                     IYVSADSVEVWTKPQLFKLDSERKPLYVAGVPADNFSADGQLWGNPIYDWPEHEKTGY
                     NWWIYRIHESFKLYDVLRIDHFKGFSDFWQVDGKAEVAKVGTWEPGPGYNLFKAVKET
                     LGDLPIIAEDLGNIDAKARKLLADCGYPGMKILEFGFFDVTGKSIDAPHRCIPNSVAY
                     TGTHDNEVVNGWYNNLEPEQQEYVDAYSNRKPIEKVSQAMLRMLFATVSDTAIATMQD
                     VLDLGEESRMNMPSTIGGNWEWRMKAEDLTQERKDFLTKMTLLYQRGNENHD"
     gene            complement(17639..19738)
                     /locus_tag="U1099_000629"
     CDS             complement(17639..19738)
                     /locus_tag="U1099_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEM4866971.1"
                     /translation="MNNNFNNFNNMDDIFNQLMGNMGGYSTERRRYSINGREVTPEEF
                     AMYRQTGRLPQTEEVAQTQAKGQIKSDGILAKLGRNLTQDAREGKLDPVIGRNKEIQE
                     TAEILARRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGLEA
                     GTQYRGAFEENVQNLVDEVKKAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPAL
                     SRGELTVIGATTQDEYRNTIHKNAALARRFNEVKVNAPSAEDTYQILKGIKPLYEAHH
                     NIELPDEVLRAAVDYSVQYIPQRSLPDKAIDLIDVTAAHLAAQHPVTDIQTLEAEMAE
                     AKQLQLEAAEKEDYEKALNEKVRIDKLQKQIDNHTEQQKVVATVNDVAQAVERMTGIP
                     VSQMGASDIERLKELKNRLAANVIGQDDAVEAVSRAIRRNRAGFDDGNRPIGSFLFVG
                     PTGVGKTELAKQLALDLFGNKDAIIRLDMSEYSDRTAVSKLIGTTAGYIGYDDNSHTL
                     TERVRRNPYSIVLLDEIEKADPQVITLLLQVLDDGHLTDGQGNQVNFKNTIIIATSNA
                     GFGYGMAEGEENQDIMDRIAPFFRPEFLNRFNAVIEFKHLDKDDLKAIVELLLAQVNN
                     TLAKKGIQLTVTEAAKGFLMEEGYDKAMGARPLRRVIENQIRDKVTDYYLDHVDVKYL
                     EADVVDGSIQIKEQSFA"
     gene            complement(20153..20563)
                     /locus_tag="U1099_000630"
     CDS             complement(20153..20563)
                     /locus_tag="U1099_000630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM4866972.1"
                     /translation="MIQPIMKDIFFLQQKSEPATQLDVQVGQDLQDTLSANAHACVGM
                     AANMIGIKKRIIIVNMGFTNLVMYNPVLISKAKPYQTEEGCLSLEGTRPTTRYQEIEV
                     EFLDASWKKISLKLTDFQAQIVQHELDHLEGIII"
BASE COUNT         6069 a         4543 c         3852 g         6283 t
ORIGIN      
        1 cgaactcttt ttttgaccag tcgagttctt tcccgctccc tcttttatta tccaattttt
       61 gtaatattgg ttgcttgaag accacgttgg ccttgctcca cttcaaatgt cactttttga
      121 ccctcttcca acgatttgaa actattagac tgaatttcag aaaagtgtgc aaatacatct
      181 ggaccgtttt cttgagcaat aaaaccaaat cccttttctg cattaaacca tttaactgta
      241 ccttgtacca tttgtgttac ctctttttat ttatttgtaa aacttatcaa gaggaaaaac
      301 aaaagatgaa actgatactt aaactctaaa tttatttaca ttccctactg taacagtata
      361 tttcctatat gtcaacgata aagggttgat aagcccaata aactcaataa tcatcgccac
      421 ggattataga atcgaccctg attaataaaa aatagataga ttgtcacgac tactagagca
      481 agaataaata gcaagacagc gtaatctatt ctttgtagag gctgttgggt ataccatgtt
      541 ctttttttat tttttccaaa acgccgcaat tccatagccg ttgaaatcgt atcaatcctc
      601 tccaaggagc tgaaaatcag aggaatgaca agggatagat tcccttttat acgatccatc
      661 aattttcctt tcttggacaa ttccaaacca cgcgcttctt gagacattcg aatcgtataa
      721 aactcttctt gaatatctgg aatataacgc atggtcaaac tgactgcgta agcaactttg
      781 taggataccc caatttgatt caagctggaa gcaaactgac taggatgagt ggtcatgaga
      841 aagataatcg ctaaaggaac tgtacaaaaa tacttcaaga gaagattcac cagataaaat
      901 gcttgctgac tcgtcaagga ataaactccc agacctgata gcaagggagt gtctgctccg
      961 taaagttcca ctccataccg tggtgcaaac aagtgaacca tcagggcatt cattgcagca
     1021 aaaatgattg tcagaattaa aacaagagag atttctttat aacgaatccc tgacattttg
     1081 aacaaggcta gagaaaatac tgcgataaaa agaatcaagc gggtatcata agtaatcata
     1141 gcaagaatgg aaactatgag aaaaaagagt aatttactga cagccgataa actatgaata
     1201 aagccagttc ctggatggta accaattagt ttttgttgac tcatcttcct ctcctttctt
     1261 cctgcatata aaacgtcgtt aattccaagg gattaactcc caacctctcc gccaagtgaa
     1321 aaattgatgt ttcttttaaa ttggctcgct caatcaccat atcatcagat aaaatttctg
     1381 ctggactggc atcttcaata atctgcccat ccaccacaac aacagctcta tctgaataat
     1441 ctagcatgag ttgcatgtca tgggtaatca tgacaatggt gtgcccttga gcatttaatt
     1501 ggtccaaaaa ctccatcatt tcccgataat gccgttgatc ttgtcctgct gttggctcat
     1561 ctagcaaaat aatttctgga ttgagaacca agatagatgc tatcgttacc cgttttttct
     1621 gcccatagga aagtgccgaa atcggccaat tacgaaacgg atacaggcca catactttca
     1681 agatatggtg aactcggtgt tcgatttctt cttctgtcac ccctcgcacg cgcaaaccaa
     1741 gagcaacctc atcaaagatc atcgtttggc taatcatttg gttgggattc tgaagaacat
     1801 aaccaatccg ttctgctcgc tctttgacag aatcttcttt gattgactgc cccttccaga
     1861 gaatctcacc ctcaccctga ataaaaccac aaagcacctt ggctaaagta gatttcccag
     1921 caccattttt tccaacaata gctagacgct ctcccttatg aatcgtcagt tgaatatctg
     1981 taagaacttg tttgttagga taggaaaaat gaagatgctt gacttctaac aaagactctt
     2041 tctctccatt ttcaaagtgt agagtcggta gagatgctat attcaaagcc tcaatatcaa
     2101 tttcccaaac tgaggagagg tgttctgcct gcgcaatcgg atatcctaag gcgcgaagag
     2161 tcgatagata taagggttca cggatgccgt tttcctgtaa caatgaggta cgtaataaat
     2221 catccagtcc tccattgaac aaaatccgac catcattaat caatacaact cggtccacct
     2281 gacgatacag tacgtcctct aaacggtgtt cgataataat ggttgtggtc ccttcttgct
     2341 tgtgcagacg gtcaatcaaa tcaatcgtat cttggcctga tttggggtct aaattagcaa
     2401 gtggctcatc aaacaacaaa ataggactct catcaataag aactccgccc agactgacac
     2461 gttgcttttg acctccagat aaatcttgtg gtcgatgtgc cagcaaatcg gtcaaagaca
     2521 gtctttctgc ccaatctcct actttttgct tcatcaccac ctggctttcc atgtcgtttt
     2581 ccaaagcaaa agctaggtcc tcggcaactg ataagccaat gaattgacca tctgtatctt
     2641 gaagaaccgt tgatacgagc aaggacttat cataaactga aagattaaaa gcttcctgac
     2701 catccaatag taaacttccg ctatgttccc ccttgtaaat attgggaata ataccattaa
     2761 gacattgtcc aatcgtcgac tttcctgaac cagatgggcc aataatcaga actttctctc
     2821 cctgataaat cgttaactga atatctttta aagtgggttc ctgttgcgct tggtatttaa
     2881 atgtaaaatc tttaaattca atgatttttt tcataggact attatagcgt aaattgactc
     2941 tgaaaaaaag gaagaatcat cagtttctcc cttttcaaat aacttcatct atgattctag
     3001 agtagatgaa ttctaacttc ctacatcaca atcacattaa aatgatgccc atcaaggtcc
     3061 gtaaaacttg ctccataaaa gccacgggca tctgtcgggc atcctgtaat ttgcccgcta
     3121 tttgcttcaa cacgctctat aagtgtatcc acttctttcc tactcgccac attaagcgaa
     3181 atcaagactt cattgccact cgctactacc tgaaatggta cgcgttgagc aaagctatct
     3241 gcctgcatca aaatcaagtt gccaccctca ggcaaagtca cagacttaat atctccaaac
     3301 gtcgcaactg caaaaccgat tgcttcataa aaccgaccag ctattcaagg tctgccactg
     3361 ctaatactca atgaaaatca aaagtaacct aggaaacgaa gcgaagatag aactgaagtt
     3421 catcaaggca agctgacaac ggataatgtt gattttcgaa agggataagt tcatccaaaa
     3481 tgacttcatc tccttctctt ttccaattga taggcttcgt atagaatgga ctgaaaaaat
     3541 ataaagaatt ctgattgcac aaaaaaattg cactgatttt tcaatgcaat tttggtatta
     3601 ctaagcattt gctggagtaa gagtttcccc aatcgcttcc aagcgttctg cccattcttg
     3661 acgagtcaag ccgttttcag agatgtatcg gttgcgctca tgagcacggc attcagctga
     3721 acagccacga acatacttgt gctcattctc ttctgatgtc aaaatgcgac ggttacagaa
     3781 tggattgcca cagttgacat agcgctcaca tggtgtgcca tcgaaccagt ccttaccaac
     3841 gacaacaggg ttaacatggt taacatcgac cgcgatacgc tcgtcaaaga catacatctt
     3901 cccatcccac aattcgccac gaacttctgg gtctttacca taggttgcga taccaccgtg
     3961 gagctggccg acatccttgt agccttcacg aaccatccag cctgagaatt tttcacagcg
     4021 aacaccacca gtacagtaaa caactacgcg cttgtccatg aatttctctt tgttatcacg
     4081 gacccattgt ggcaactcac ggaagttgcg gatgtctgga cggatcgcac cacggaagtg
     4141 accaaggtca tactcgtagt cattacgtgt atccaaaaca actgtatctt cgtccaagag
     4201 agcttctttg aactcttttg gtgataggta ggcacctgtt gtcacgagtg gatcaatatc
     4261 gttgtcaaag tcgttgtctt ccaaaccaag gtgaacaatt tcttttttat aacgaacaaa
     4321 cattttcttg aaagcttgct cattttcttc atcaatctta aaccacaaat cgctgaacaa
     4381 tgggtttgca tgaacgtagt ccatgtactt ttgagttgtt tcatagtcac cagaaacagt
     4441 accgttgatt ccctcatcag caatcaaaat acgtcctttg agtccgatag acttacagaa
     4501 agctagatgc tcagcagcat attctttggc attttcaatt ggaacatact tgtaatagag
     4561 tagaacgcga atatcttttg acatagtgtc tccttttcaa tctttgcaat agatgataaa
     4621 actctgtcat ttctaataaa tgatacctta cgattatatc gtttagcccc tcaaaagaca
     4681 aaaatctgaa ctgaaaattt agaacctgta ttcgcgggtt caatgcttat atgtaaaaaa
     4741 tacggtaaca tggatggcat gctaccgcat ttattgaagg ttcgctataa cttcttagtc
     4801 gtctaattct gttttcagtt gtcggagttt ttccaaggta tcatgtccaa tcagttggtc
     4861 cacttttcgc ttatggcgtc tagcaatatt ttccttccat ttatcttcaa taggattatg
     4921 tggcttctcg attttccttt gatctttgat ttctcttgcc tcctgccatt ccactccttt
     4981 aataacttct tcccaatagc tggcaatggc agagcagaga tagagttgga ccttattcaa
     5041 ccagtgtcca aaaaaagttt tcggagcatt ttcctccact agccgatagg tcctttggat
     5101 ttcaataagt tgactttggt atttttccca ctggagcata gctttactcg gcttttttcg
     5161 ataaagttga cgctgtttga ttagagctat atacggtgca acaaaacggc tcgtccgtat
     5221 cttgataaaa tttctggagg agcgaatggc caccagcatg cctgaggcca ataagcgttc
     5281 aacctccctt gcctgattat ctggaaaacg gtaatggact tgccacccct cttcttcaac
     5341 cactaagcga atctgcattc taccattttg ccaactcgcc tccacctctt gcttttcatc
     5401 ccataacttt tgaaaaaaga gaagaaggta gtagctttgt ttactaagtt cataccaact
     5461 gttcgtgtga taaaggtagg tccgaatcgc tcctttcata taaaattcgc catggaaatc
     5521 ataaaaagtt ggctgtttat atgtagattg tgatgggtgg ttactaactc ggaaaacctt
     5581 ataataataa ctattccaaa gaatgcagcc ataaattgat ttcgtcgttt tcgattgata
     5641 aagacttaaa acttcctcgt caggattgag tatagaatga ataatcggtc tcaacttttc
     5701 ttctgtccag accatacttc tccccttgaa cacatgtttt tgtttgtttt atattattat
     5761 atcatatatg tattttttgg tattgaaaaa aacctagaat tttttctagg gaaaataaaa
     5821 acttaatctt tagacaaact acctgtcttc gtttgagatt ttgcataaac agccaaaagg
     5881 agtgtccctg caatagcaac tgtaatactg tttgcaatac ctgccacaag tccctgtgca
     5941 aaaaccttat tcgccgcctc actgtagata agaatatcta aaactggggc aatgactccc
     6001 caagcaagca cattcgccgc aatctgaaca aggttaaaga aaagaatatc tttcccttca
     6061 aaaatccctt cttgaatacg tagacgtttt ttggtaagac caacaaccaa accaaagacc
     6121 cctgatgcca aaatccatga ccaccatgga ccgtaagtca acgaatcctt aagcgtgtgc
     6181 ccaatgaaac cgaccaagaa gccaactaca ggaccaaata caactgccaa tagcgattga
     6241 acagcgtatt gaagctggat agaagtattt gggacaaagg ttggaatatt gattaaatga
     6301 ccaataacca caaagagggc tgcgccgata ccagtagcaa cgactgtttt gattgaatta
     6361 tttttcataa ctatctccta aaatttcttt aattactaat acgtttgatt tcaatatgcc
     6421 agttttgacc tacaccgaca ttataagcct tggcaaatga gccctgatta atcgcaacac
     6481 ctacacgata gagggaattg atataaatca atggctgtcc gatacgtaca tccgcaaagg
     6541 acttcccgta ggttacttgg ttttgataca caagcatgtc gttattgtat atggtcactt
     6601 caaagcgatc ttcaaactgc ggttgcaagg tgtaaaattc ttccctcgta atcgaggtcc
     6661 aaagcgagcc gaatcgaaca tccagaatat caattgcacc ttttacatag tccgaaccaa
     6721 cttccgttgg gactgttgga atttccacaa tcttatccac gcttaattct ggaccaactt
     6781 cttcaaagct aatgtgtcca gaagccaatt tagctcccgt ataggcataa acatctcgac
     6841 catggaaggt gtacgaatgt tctgtattgg cacggcgatt ggccacctct gaaatttctc
     6901 gaaccgcctt tatccccaca tattttttga tgaaggatag cgtcccatta tctggcgtta
     6961 caatgtagtg attttgttcg gtcaaagcta ctacactctt acgttttgaa ccaactcccg
     7021 gatcaacaac cgaaacaaat gttgttccct ccggccagta ttcgaccgtt tgaaagaggc
     7081 gatatgaacc ctcaaagata ttgtacggag taatatcatg agttagatgg tgaacaacta
     7141 aatctctcga ttcttggaga gcaacaccaa tcatcgctga aactgcacca tcaactaaac
     7201 caaaatctga ttgtaaaaca agtaaattat ttgacataca ttctcctaat cttattaccg
     7261 ttctatgata acagaaaacc tgtgaccatt caaatagata tttccaaagc ctggctatag
     7321 tttttcctta tacctcatct atcaagcact ctactatcag ccaccgaaaa ctcgcaaatt
     7381 tttccatcgt accttcttcg aacttaagct aaaatatccc cacttctcta gttccaacaa
     7441 ctatcgtctt tgagtaattg gatataaaga aaaccgccta gcgcagttcc aatttccaaa
     7501 cttttaagcc aaaaattgtc cttgtggaca gctgaaaata aagaaaacca gctaccgctg
     7561 gttctcattt ccaaccttcg acgtcccacg tacaatgaga atcatctacc gacgatttca
     7621 atttccagcc ttttagtata aaaaaagtgc taccagcact tttttatact aaaacccatc
     7681 cacattcgtg tagatttttt ggacgtcttc gtcgtcttct agggcatcgt agagtttttc
     7741 aaatgttgcg aggtcatcgc cttcaagtga tacttctgat tgtggaatca tttcaagttc
     7801 agtgacgttg aactcttgga ttcctgattc tttaagtgcc acgattgcct tgtgcaaatc
     7861 agttggggct gtatagacag tgattgtgcc gtcttctgct tctacgtcat ccacttcgac
     7921 atctgcttca aggagcaatt cgaagatggc gtctgcatca tcacctgcaa agacaaccac
     7981 acctttttta tcaaacatga atgatacaga accagatgcc cccatgttac cgccgttttt
     8041 accaaaagca gagcgcacgt tagctgccgt acggtttacg tttgatgtca aagtatcaac
     8101 gataatcata gaaccatttg gtccaaagcc ttcgtaacga ccttccacga aggtttcgtc
     8161 tgtgtttcct tttgccttgt caatggcctt gtcaatgatg tgttttggaa cctgcgcttg
     8221 cttagcacgg tcgataacaa atttcagcgc tgagtttgtt tctggatctg ggtcaccctt
     8281 tttcgctgct acatagattt caacaccaaa tttggcgtaa acttttgagt tagcaccgtc
     8341 ttttgcggtt ttcttggcta caatattggc ccatttacgt cccatgggga acctcctaca
     8401 ttttataaaa tcgctttatt ataccataaa tggcaaggat tgagaagatt tttaaaatct
     8461 tcttctctat acagttgaca tgtatagttt ctcgatatat aatatatagt acaactatac
     8521 atttgaacta tataggagaa gccaaatgaa aaggaacaag catctcccct taacggagac
     8581 aacctactat attctattag ctctcttgga acctgcccat ggctaccata tcatgcaaaa
     8641 agtagaggat atgagtgatg gtgatgtgaa aatcgctgca ggaaccatgt atggagcgat
     8701 tgagaatctt ttgaaactaa aatggatttg ttccgttcca agttccgata aaagaagaaa
     8761 agtctatcaa gtaacagcaa cgggccaaga gattattaaa ctggaaacac agcgtattca
     8821 aaaactacat catattgcca aagaattagg tttttaagga ggaagccatg ataagacaca
     8881 aattattcac cagtttacaa gaggaagaac attggattaa ttcgattcaa tccgaaggtt
     8941 atcagcttgt caaagtaact ccgtggacag cagcctatca ttttgaaaaa tgtagtcgaa
     9001 cccctcatcc cgtacgcctt gattttcatg aacatattgc aaaaggagaa tattcaaatt
     9061 acctctctct atttgaagac tgtggttggc aacctataca aggtagtcgt agatgtggca
     9121 tccattattt tcagcaaaca atcgaagctt caagcactga aattttttct gataccgaat
     9181 cgaaaaaagc attctattca cgctaccaaa attatgccta ctcctatttc ggattatttc
     9241 tgactttatt ttttatccat taccaagttg gactccaaaa tggatacaca ctttggaatc
     9301 ctaaatcctg gtacctaact ccagaactat gggaacgaac tggtaccagt ttttggtttg
     9361 gttttctctt tgaaacccca ttcgcattgt ttagaagtgg cattattccc ttatttttcc
     9421 ttgcttggtc tatttatttc ctccatatcg ctgaaaaagg taaaaaagaa gcgaagaaat
     9481 tagaaaaata ctgatggcat actatccaaa agaaaaagat tgaaaagagt tgactacaat
     9541 catcttttca atcttttttt actttttaca actcaactgt cgcaacttga ctatcttgac
     9601 taacctgtcc aagatttcca aggctgacag atttgatttc agcagtattt gtgaaggcaa
     9661 caacgatggt tgtttcacgt cctgctgagc gaatggcatc cagatctgcg acaaggagca
     9721 agtctcctgc ttcaacgcgt tgcccatctg ttaccttagc tgagaatggg gcaccgttta
     9781 gtgcaaccgt atctaggccg acgtgcacca agacttctac acccttgtca gacaaaatcc
     9841 cgacagcgtg tttggttggg aagacactgg ttacgatacc tgaaactggt gcgtaaacat
     9901 tgccggagct aggctcaact gcataaccgt cgcccatcat tttttgtgag aagaccggat
     9961 ctttcacttc tgtgatggca atggcttgcc cttcagcaac agagtaaact gtctccgtca
    10021 cacctttgaa ctgagtctca ggagcaactt cttctgtcgc aacaatttcc gtacgaggga
    10081 tggctacgcc tgattctagt aagtcttgaa tgtctgattt gagaacatct gcttttggtc
    10141 cgtagactgc ttgaacacct gagtctttga taatcaagcc catagcacca gcctgtttcc
    10201 aagcattttc atctccaacc tgtgccacat ctttaacact aacgcgaaga cgagtcatac
    10261 aagcatccac atctgcgata ttatcacgac caccaagcaa gttgatgatt tgaaccactt
    10321 gtgaattggc atccgcagta tctacgtttg agtttgaaac cacatccgtt gtttctgtat
    10381 catagttacc attgcgtcca gctgttgcta ggttgaattt cttaatcata aagttggcaa
    10441 tgaagtacat accaaccgca aagagggctg ttacccagat aaagttaata acatccatac
    10501 cgataccagc tttgatcgcc atcggtgtac gtgtaaggaa ttcgatatta ccaaatgagt
    10561 gcatacgcaa gttgacaacg tcagccatgg caaaagccgc accctgtaca aaagcataaa
    10621 cgacatagag tggcattgct gcaaacatga acataaactc aattggttct gttacacctg
    10681 tcaagaatac ggcaagtgct gaagaaataa acataccttt gtacttatgt tttttatctt
    10741 catccacatt gcggtacata gcgtaagtca atcccatcaa aatccctgat gaaccaatca
    10801 tttgaccaac tttgaaacgg gctggagtaa tgtttgcaag aaggtcattg tactgagcca
    10861 tatcaccagc gtctttgagg ttgatcaagt ctgtaatcca agccaaccag agcggatctt
    10921 gtccaaatac ttgtgcacct gcttgtgcac ctgtcaagat gtcataggta ccaccaagtg
    10981 atgtatagtt cattggaatg gtcaacatgt ggtgcaaacc aaatggaaga agcaaacgtt
    11041 ccaaggtacc ataaacaaaa ggtgcaagaa ttggtgctgt atcctgtgag cttgcaatcc
    11101 acttaccaaa gctgttgatt cctgtttgaa caactggcca aaagatagct aatcctagcg
    11161 ctacaagaac tgagcgatag ataaccacaa atggtacgaa gcgtttaccg ttaaagaagg
    11221 tcaaaacatc aggtaatttg cggtaattgt agtatttgtt ataggttgtt gcccctacaa
    11281 aaccagcgat gataccaacg aagacaccca tgttcagagc aggctgaccc aagatgttga
    11341 cgaagtagcc tgaaaccgga atctctgttc caaaaacagt ggttacggtt gccgctgcat
    11401 ctgcaagcat gtcagtcgtc actccaaaga agtgacctgt aatcaagtta ataaggatga
    11461 aggcaagacc tgctgagaag gcaccaccag ccttttcctt agcccagcta ccaccgatag
    11521 ccaaggcaaa gagcaaatga aggtttccga taatccccca accgatttgg gcgataatat
    11581 tcccgatacg aatcaagagt tctgattctg ggctgattaa cggaatagag tttccgatag
    11641 aaaccatcag acctgctgca ggcataactg caatcacgac cattaaacac ttaccgaatt
    11701 tttgccaaaa ttcgaaacta agaaattttt tcattttttc tcctttattt tttggctctg
    11761 tgccatttta tacattaatt tcaacttcca caccaaaatg gtctgaaata atcggacgat
    11821 tctctccatt gaaaatcact aagctggata aaacttcctt tttctgattt aagaaaatgt
    11881 aatccaatcg tttttcctga ctgtgaccag cccagccatc gatagccttt tcaaccgtaa
    11941 tcccctggtc tttttccatc gctagattgt atgaatcata aagcccgagc tctgtaatcg
    12001 cttcgtaggc ctcgcgatct gagaaggcat cggtattaaa atctcccatg agaatcttaa
    12061 aacgtctatt gccagtccgt tccacaatcg tacgaacgtt atcaagctgg tcttcctctt
    12121 cactccctgg aagattgatg tgacaagaat acaggtcgat caactggtct tgatagaaga
    12181 ccgtcaagcc cataattttt cgtgatagga tggaatcaac agatttattc tgactacaat
    12241 aaaatggatc cacttcataa actggcaact tggtaagaaa ggcaatccct tcttcgtact
    12301 tatcgtagcc gatatgtgag ttgctccaat aatatgaata atcaaattgt cccaaggctc
    12361 ttaatttttc taataaaatc aagccataat tatccgcctt caagtctttc gttaccaaat
    12421 cagcagttat gagctgattg acctcttgaa gagcgatgac atcataagcc ttttcagcta
    12481 ttgttttggc taaaacatct agtttttcat cctgcctttc ttctaaccag gcatggacat
    12541 taattgttag taatttcatt gctccccctc tctctgaaaa tgcgtaaccg ttttctttta
    12601 tagaaatagt ataacgtaaa cgtttgcatt agtcaatagc ttttccgtaa tatttcaaac
    12661 ttttacgtaa aagttgtgta ttctactaaa taaaatagat gtattgcccc tttctggctc
    12721 ttttatgata cactaatggg tagaaagaca ggaaactatg agtaagtata aaaaagttta
    12781 tcaagatatt aagaaaaaaa tcgaagagca ggtttggtca actggacagg ccctaccgac
    12841 agaaaatgaa ttaatggaag tgtattccta ctccaaggac accattcgca aagcactatc
    12901 tcttcttgaa atggatggtt atattcaaaa gaaacagggg aaatcttcta ttgttatcga
    12961 acatggtcta atgaaggacc aatatctttc tgaaattaaa acagcgggcg aattgaataa
    13021 aagatcacag caccaaattc agacccagct tacttcgctt tatattattc aaggccaaga
    13081 agatttaatg tcaactttcg aagttgatga taaggtagat ttctatcgag tgagtcgggt
    13141 acggacaatt gacggtgagc ggttggaata tgacatttcc tatttcgacc gtagggtcgt
    13201 tccatatctc agtaaagaaa ttgccgaaag ctctatttac cgctacttag aagaagaact
    13261 ccatttaaca atttctcatt ccagacgtga aatctccttc cgttttgcaa acgaagaaga
    13321 aaaacaattg atggacttag gagactatga catggtcgtc gtggtcacta gtatcaccta
    13381 tctttcaaat ggtcaagctt tccagtatgg aacaattagt taccgtccag acaaagttag
    13441 ctttgtatca atggcgaaac ggtaaaagga aactttcgat ttttttgacc aagtgtacaa
    13501 aacttctaga attttatagt tataaaaaga atgtctccgc ttttgcagag acattctttg
    13561 catttatata ctaaggagag caccaataat ggtaaaaact ggtagttttt attcttcaac
    13621 acataggaag aattgatctg ataagttttg gagtggttca atcaactttc ctactaattg
    13681 ttggaattca attagttact gtgccagatg tcttgattgt actgttcaat ggtacggtca
    13741 gatgagaaga atccagcttt ggcaatgtta tgaacaactt tcttgttcca tgaatcttga
    13801 tcttcgtaat ctgcaaagac ttgttctttt acttcaatgt attcagccaa gtcaatcaag
    13861 gtcatgaacc agtctttcga aatcaattca tggtgcagac gaccaagacg ttcttcgtta
    13921 ccaagtgctt ttacctcatc gctaacgata aagtcaactg cacgtttgat attggcatcg
    13981 ccattgtagt agtccgcaga tacatatcca gccttatcgt acaagtcgat gatggtatct
    14041 gaatctttac caaatgtata gatgttgtcc ataccagcca actcagcaat ctcaacgttg
    14101 gcaccatcca tggtaccaag tgtcaacgca ccgttgagca tgaatttcat gttaccagta
    14161 cctgacgctt ctttagaagc caatgagatt tgttctgaaa tatctgttgc agggatgagt
    14221 ttctcagcaa ctgttacgtt atagttttcc accaaatgaa cgttgaggta cttgctcact
    14281 tctgggtcat tgttaatcaa ctctgacaag caaagaatca agtgaatgat gtcttgtgca
    14341 atgatgtagg caggtgccgc tttaccaccg aagataacag tgattttacg tttcggaagg
    14401 ttaccattct tgatttcaag gtatttatgg ataacataca aggcattcat ttgttggcgt
    14461 ttgtactcgt ggaaacgttt gatttgtgta tcgatgatag agttttcatc cagctcgata
    14521 cctttattgt ctttaaggta acgtttgaga gccaacttat tgttgaactt gatttctgcc
    14581 aacttagcat gcacttcctt gtcatcagca aaggcaagca atttttcaag tttagtcgca
    14641 tctgtcaagt attcatcgcc aatcaattct ttgatgtagt cagcgaggtc ttggttggca
    14701 aattccaacc aacggcggaa agtgataccg tttgttttat tgttgaattt ttctgggtaa
    14761 agctcgtaga aagctttcaa ttcagagttt ttgaggattt ctgtgtgaag cgcagctaca
    14821 ccgttcactg agttagagaa gtggatatcc atgtgagcca tgtgtacacg acctgattca
    14881 tcaatgattt ggactgctgg gtctttaatt tctgcacgaa taagggcatc caattctttg
    14941 atgatatcaa ccaagtgtgg caccacttct tcaaggaact caagtggcca tttttcaagt
    15001 gcttccgcca agatagtgtg gtttgtgtaa ccagtcatat tcttaacgat ggcaactgct
    15061 tccgcaaatt caataccatg cttttcagtc aaaagacgga tcaactcagg aatcaccatt
    15121 gatgggtgag tatcgttgat ttgcacataa gcgtagtcag ccaagtcatg caagtttgaa
    15181 ccgcgctcaa ctgcttcatc aatcaaaagt tgagcagcat ttgataccat gaaatattgt
    15241 tggtagatac ggagcaattc accattctta tcagaatcat ctgggtacaa gaagagagtc
    15301 aagttttctt taatatcagt cttgtcaaat gaaataccgt ctgtaatcaa gtcatagttt
    15361 actgactcaa tatcaaacaa attgaggtag ttcttagtct cttttttata gccaagaata
    15421 tcaagacgat ccaattttga tttcaaagtg aagtttttga atggaacatc atagctgata
    15481 gttgttggaa tcaaccaaga atcattttca atccagaagt ttggctctgc ttcttgctca
    15541 tttttcttaa atacttggcg gaaaagacca cagtggtagt tcagaccaac accttcaccg
    15601 ttgatagcaa gtgttgacat agaatcaacg aaacatgaag ccagacgtcc caaaccaccg
    15661 ttaccaagtg atggttctgg ttcaacatct tcaacttgcg ccaatgattt accagctgct
    15721 gccaattctg cttgaacgtc tttgtacacg cccaagttaa tcaagttgtt tgacaagagt
    15781 ttaccgataa ggaactcagc tgagatatag taaactttac gctttcctgt gttttttggt
    15841 tttgaagctg ctgcatcttt tacgtagttc aacagttgaa gatagatttc ttcatttgtc
    15901 aaatcagcta atttcttcga agtatttgtt tctgcaaatg ttgtaaattt agtcatggtt
    15961 ttcatttcct cgttggtata gtaatgtcat ttttgtcaag aagtctttgc gttcttgggt
    16021 caagtcttca gctttcatgc gccattccca gtttccaccg atagttgacg gcatattcat
    16081 tcgactttct tcacccaagt ctaggacgtc ttgcatggtt gcaatagctg tatcactcac
    16141 tgttgcaaac agcatacgga gcatagcttg gctaactttt tcaatcggct tacggttgct
    16201 gtaggcgtcc acgtattctt gttgttctgg ctcaagattg ttgtaccagc cattgacaac
    16261 ctcattatcg tgagttccag tgtaggcaac tgaatttgga atgcaacggt gtggagcatc
    16321 gatactctta cctgttacat caaagaaacc aaattctaag attttcatgc ctggatagcc
    16381 acaatccgca agcaatttgc gagctttagc gtcaatgttt ccaaggtctt ctgcaatgat
    16441 tggaaggtct ccaagtgttt ctttgacagc cttgaagaga ttgtagccag gacctggttc
    16501 ccaagtacct acttttgcca cctcagcctt accatcgact tgccagaagt ctgagaaacc
    16561 tttgaagtgg tcaatccgaa ggacatcata aagtttgaaa ctttcatgga tacgataaat
    16621 ccaccagtta tagcctgttt tttcatgttc tggccaatcg taaataggat ttccccataa
    16681 ttgaccatct gccgagaagt tatcagctgg tacaccagct acataaagcg gcttgcgttc
    16741 actgtccaat ttgaatagtt ggggttttgt ccacacttca acgctatcag cagaaacata
    16801 gattggcatg tctccgatga ttttgatgtg gtgtttgttg gcatattctt tcaattgttt
    16861 ccattggctg aagaagaaat actgtgtgac tttgtagtag tcgatttgat ttgccaattc
    16921 taagcggtat ttatcaagag cttcctcgtt gcgagctaca acttttttat cttcccattc
    16981 ttggagagct ttattaccaa agtattcctt gatagccatg aactccgcat aatctgtcaa
    17041 ccaagatgag ttagcctttt caaactctag aaaagcagct ttcttcttat catcttgtaa
    17101 aaatgcttga actgccttct ccaaaattgg tcggcgaact tgatagataa gagcatagtc
    17161 tactttttca ggattatctc caaaatttac atcttggaaa tcttctgttg ccaataagcc
    17221 atcttcaacc aagaggtcaa agtctatcaa gtgtgtattt cctgcaatag cagagaaaga
    17281 ctggtaagga gaatctccat agcttgtagt tgttagtggt aaaatctgcc agtaggtttg
    17341 cttggtttcc accaagaaat ctacaaaatc ataggcagac tgtccaaaag taccaatacc
    17401 aaacttacct ggaagtgagg taatatgcat caaaattcca ctggtacgat ttgtcataaa
    17461 aacgttttcc tttctgtcgg ttacgaatat agtttaacgc aaacgtttgc acttgtcaag
    17521 gattttataa aaaaagtttt aatgtactct agaaagccta ttttatagca tttgttagcg
    17581 aataacaaaa aagaaaaatc agatatttct atccgatttt tcaaattctt cactattttt
    17641 aagcaaagct ttgctctttt atttggatgc ttccatcaac cacatccgct tccagatatt
    17701 tgacatcaac atgatccaaa tagtaatctg ttaccttgtc tcgaatctga ttttcaatca
    17761 cacgacgcag aggacgggct cccatggcct tgtcatagcc ttcttccatc aagaatcctt
    17821 tggcggcttc cgttaccgtt aattggatgc ctttcttcgc cagggtatta ttgacctggg
    17881 ccaagagtaa ttctacaatg gctttgagat catctttatc caagtgcttg aattcgataa
    17941 ctgcgttaaa acgattaagg aactccggac ggaagaatgg tgcgatacgg tccatgatgt
    18001 cttgattttc ttcaccttct gccattccat aaccaaaacc tgcattagaa gtcgcgataa
    18061 taatggtatt tttaaagtta acttggttgc cctgtccatc agtcaaatgc ccgtcatcta
    18121 gcacttgcaa gagaagggta atcacttgtg gatcagcttt ttcaatctca tccagaagaa
    18181 cgattgagta cggattgcgg cggacacgct cggtaagagt atgggaattg tcatcgtagc
    18241 caatgtagcc agctgtggta ccgattaatt tggaaacggc tgtccgatcg ctgtattcag
    18301 acatatccaa acgaataatg gcatccttgt taccaaacaa gtctagtgct agttgcttag
    18361 ccagctctgt cttacccaca cctgtcggac cgacaaagag gaaggaacca atcggacggt
    18421 tgccatcatc aaaacctgca cgattccgac gaatggcacg agatacagct tccaccgcat
    18481 cgtcttgacc gataacgtta gctgccagac gatttttcaa ttccttgagt cgttcaatgt
    18541 cagaagctcc catttgagag actggaattc ctgtcatccg ctcaaccgcc tgagccacat
    18601 cattaacagt cgccaccact ttctgttgct ccgtatgatt atcaatctgt ttttgtagct
    18661 tatcaatacg gactttttca ttcaaggctt tttcataatc ctctttctca gccgcttcca
    18721 actgcaactg ttttgcctca gccatttctg cttccaaggt ctgaatatct gtcacaggat
    18781 gttgtgcagc caagtgtgca gcggtcacat cgatcaggtc aatagcctta tcaggcaagc
    18841 tacgttgtgg aatatattgg acagaataat caaccgcagc tctcaaaacc tcatctggca
    18901 attcaatatt gtggtgggct tcataaagcg gcttaatccc tttcaaaatc tggtaggtgt
    18961 cttctgcgga cggagcatta accttgactt cattgaaacg acgagccagt gcggcgttct
    19021 tatgaatggt attgcggtat tcgtcctgtg tagtggcacc gataaccgtc aattccccac
    19081 gggataaagc tggtttgaga atatccgcta aacccttgga acctgagtct ccgccagtgc
    19141 taccagctcc caaaatctga tgaatttcat caaagaagag aatgatattt ccagcttttt
    19201 tgacttcatc cacaagattt tgcacatttt cctcaaaagc tccacggtac tgggtccctg
    19261 cctccaagcc agaaatgtca atggagatga tttccttgtt cttaatggct gctggcacat
    19321 ctccgttgac aatggcttga gccaatcctt ctactaccgc agttttacca acaccagcat
    19381 caccgaccaa aacaggatta tttttcgtac ggcgagccaa aatttccgct gtttcttgga
    19441 tttccttgtt tcgtccaatg actggatcca acttaccttc acgcgcatct tgggtcaaat
    19501 tgcgccccag ttttgcaaga atcccatctg actttatctg acctttagcc tgtgtttgag
    19561 ccacttcttc ggtctgtggc aggcgaccag tctgacggta catagcgaat tcctccggtg
    19621 tcacttcacg cccattgata gaatagcgac gtcgttctgt actgtatcca cccatgttcc
    19681 ccataagttg attgaaaata tcgtccatat tgttaaaatt attgaaattg ttgttcatag
    19741 tagttacctc gtttaagttt cagttatttt tgacttttac tgacctttgt cagaaaataa
    19801 aatctaatag ggtataatcc tgtctttcat agcaacctcc tcagaagttt gtcttttact
    19861 gaccaatagg ttcaagagta agtcaaaccg ttctcgtttg acctaaactg accttttgtt
    19921 caaagaattt gtaaggccct ttaccttacg aatcttattt tacatcattg gtcagtaaaa
    19981 gtcaatgatt ttgtttgacc ttttttgact ttttatagtc gaatgaatta gctttcagac
    20041 aaggaactga ggcgcaggct atactaaagt atggcaaggc aaaagtgacg atgtatcaaa
    20101 gttaattcaa atgactataa aaaaatctac ctgacggtag atttggtagc tactagataa
    20161 taatcccttc cagatgatct agttcatgct gaacaatctg ggcttggaag tctgtcagct
    20221 ttaggctgat ctttttccaa gaagcatcca aaaactccac ttcaatctcc tgatagcgtg
    20281 tggtcgggcg agtaccttcc agcgacaggc agccctcttc tgtctgatag ggcttggcct
    20341 tgctgatgag gacgggattg tacatgacta ggttggtaaa acccatattg acgatgataa
    20401 tccgcttttt gatgccaatc atgttggctg ccatgcccac gcaggcatga gcgttggctg
    20461 acaaagtatc ctgcaaatcc tgaccgacct gcacatctag ctgagtcgca ggctcagact
    20521 tctgctgcaa gaagaaaata tctttcataa taggttgaat catggggtag ctccttgctt
    20581 tgttatgaat atgtactttc tattaatcgg agagtatcgt tatcaatagc gaattcatag
    20641 acagaatctc tatattttat aataataaga tttttctcac tattaaattc tttatccaac
    20701 tgtttaagaa tctccccata aatatctaat ttattttcca tattaag
//
LOCUS       DASFSW010000022        20196 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.022, whole genome shotgun sequence.
ACCESSION   DASFSW010000022 DASFSW010000000
VERSION     DASFSW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20196
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.022"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(1..1547)
                     /locus_tag="U1099_000631"
                     /pseudo
     CDS             complement(1..1547)
                     /locus_tag="U1099_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638990.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            complement(2348..4525)
                     /locus_tag="U1099_000632"
     CDS             complement(2348..4525)
                     /locus_tag="U1099_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910563.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEM4866973.1"
                     /translation="MNSKIFSLRKSKMGLVSVAIAFLWIGTGMNMETAMAEETDATAL
                     ETQLESKESSLTNTVSENAEAEEVTDEVPSEEKKSEEMEDMEEELPFIENHLEVAPIQ
                     AGDQTISGNTTPGGYVAITIDGEAITSIENILEADDKGDFSYRLSKPLAHSQTVEISA
                     LPKQFWTLEADSEERKVVVRTNRHPEAYEIPAKRLEKTSNGMHQVFIEPVFEHTSKVI
                     GHTSVKGSVYLSINGSFVSDKTLIDPKDGRFEVTFSESLAGSKFKADDRLVLSFVSED
                     GQPVITNTIVKPLVKEKVSSQMTVKPLSSATSVLEGTTFPLGRVHLYNADTSEFIMEA
                     IADETGHYKIALPALQSEDKYYRLTHNQQEDLVSVHLDTVDGSSILLDKSVMASLATY
                     LQDADMDEATDEDPIIVPKLHNKKDYIVGRTIHLNAYVRMVSSIKGKQYPPVQVDELG
                     FFGFQIQDLQLPFEKGERIRFEIIDPVTNNIIASKEEVVGQYLEDEDVMDLPFQVEKV
                     TTDHGYISGKTAPDVMIELVSTQNGEEIIGKTSTDSTGRFEFDLGSRVLKNGETLSFR
                     AFDKEGEQVAWEVVTVQKGNGHRINKPDKKDEKEEQPSKEITKNIEQSDTLEQTTLPP
                     VRQTLTDKKVEQNAEPSKEETVSIFDDSKKDMPTKQEKMARTVRDKGTKGNVSVHDSG
                     ENTQVQSLPKTGEKTSLVANIMLSIILFLFALFIGKKKITESE"
     gene            complement(5127..6062)
                     /locus_tag="U1099_000633"
     CDS             complement(5127..6062)
                     /locus_tag="U1099_000633"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEM4866974.1"
                     /translation="MSKFLVFGHQNPDTDAIASSYGWAHLEREVFGRDAEAVALGTPN
                     EETAFALDYFGVTAPRVVESAKAEGVSQVILTDHNEFQQSIADIKEVEVAAVIDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRAFKENGVTPPKEVAGLLLSGLISDTLLLKSPTT
                     HTTDPQVAAELAEIAGVNLEEYGLTLLKAGTNLASKSAEELIDIDAKTFGLNGNDVRV
                     AQVNTVDIAEVLERQAEIEATMTAASAANGYSDFVLMITDIVNSNSEILALGSNMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTDAFNA"
     gene            6200..7897
                     /locus_tag="U1099_000634"
     CDS             6200..7897
                     /locus_tag="U1099_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEM4866975.1"
                     /translation="MKFSDYTYVRPDYETIKAQFSDLTDQLAQASDLETTRTLVAAVT
                     KLLNLVDTQYNLWMIRHTIDMNDEFYNEETKFWNEYSPLFEELTTNYYRVIVQTPYKA
                     ELADILPETFFMQAENKLKTFSSDVIPLFQKENELTDEYSKLIAGAEIDFQGQTYNLA
                     QMGPFGQSTDREVRKAASAATTAFFESKEADFDRVYDELVKVRTEIAHKLGFKDYVEY
                     GYLKMNRFDYNCDMVKVYREEILKHIVPIVQNLRERQAKRLQVPSLKHYDLNLEFLDG
                     NAVPQGDPDFILSQAQDMYRELSAETGEFFDFMIEHELLDLVAKPGKNSGGYCTYIPD
                     FKSPFIFSNFNGTSGDIDVLTHEAGHAFQVYRSRWIQSPEVVWPTYETCEIHSMSMEF
                     MTWPWMDRFFKEQVDKYKFTHLASALLFLPYGVLVDHFQHEVYEHPEMTPAERKATWK
                     KLQDLYLPDRDYSESEALNRGIFWYRQGHIFASPFYYIDYTLAQVCALQFWKRTQVDH
                     DENAWEDYIRICDLGGTKSFLQVVEAANLQSPFKEGALESTAKAAADWLNAVKDSDL"
     gene            complement(8006..8797)
                     /gene="pflA"
                     /locus_tag="U1099_000635"
     CDS             complement(8006..8797)
                     /gene="pflA"
                     /locus_tag="U1099_000635"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEM4866976.1"
                     /translation="MMTEIDYRKVTGLVHSTESFGSVDGPGVRFVVFMQGCHMRCQYC
                     HNPDTWDLVNPAATERTAEDVLNEALRFRMFWGKEGGITVSGGEATIQIDFLIALFTL
                     AKEKGIHTTLDTCALTFRKTERYLEKYSRLMEVTDLVLLDIKEMNPEQHRIVTGHSNK
                     TILECAQYLSDIGKPVWIRHVLVPNLTDRDEDLIELGKFVKTLKNVERFEVLPYHNLG
                     EFKWRELGRPYPLEGTKPPTKARVENAKALMETETYQEYLNRIKG"
     gene            complement(8918..10255)
                     /locus_tag="U1099_000636"
     CDS             complement(8918..10255)
                     /locus_tag="U1099_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEM4866977.1"
                     /translation="MEDPGSQTIYLQLLILFLLTLFNAFFSASEMALVSLNRSRVEQK
                     AADGERKYIRLLQVLENPNNFLSTIQVGITFISILSGASLANDLGAVFAKWMGNSTTA
                     QTAGYWLALAMLTFVSIVLGELYPKRIAMNMKENLAVVTAPVIIFLGKIVSPFVWLLS
                     AATNLISRITPMNFDDADEQMTRDEIEYILTKSEKTLDAEEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIEDDTATIMAAILKQNFSRIPVYDGDKDNIIGLIHTKKILSEAFSN
                     GFDNLNIRRIMQEPLFVPETIFVDDLLTSLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKTEIFVREIADNTYIVQGNMTLNDFNEHFEMELESDNVDTIAGYYLT
                     GVGTIPSQDEKVSFEVDSKDHHLVLINDKVKNGRVTKLKLLITPLEKEETDKD"
     gene            10388..11167
                     /locus_tag="U1099_000637"
     CDS             10388..11167
                     /locus_tag="U1099_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4866978.1"
                     /translation="MIISMKNVHYKRQGKTMLEDINWEFKKGERWAILGLNGAGKSTL
                     LRILMAEFWKTSGDLTVLGVEFGKGDIPSLRTKIGVVGSFLAERFPIDLTAEQIVLTG
                     KYKSSILYREYGQAELDEAIDMLKTIKAEHLIGRAYASLSQGERQLLLIARSLMEEPA
                     ILILDEATVGLDLLARERLLHHIDYLCQLPTAPAIIYVTHHAEEITDNFTHVLLLKDG
                     MVLAQGPKEDILTPELLSDFYGNQVELIDLGEGRLFIKPIL"
     gene            complement(11183..11737)
                     /locus_tag="U1099_000638"
     CDS             complement(11183..11737)
                     /locus_tag="U1099_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM4866979.1"
                     /translation="MLRPLHMAHAFLDEILTDQDLAVDATMGNGHDTLFLAQRAGKVV
                     AFDIQEQALTTTAEKLEKAGLTNAQLVLTGHENLDQYVDECKAAIFNLGYLPSADKSV
                     ITLPATTLQAIEKVLNRLVVGGRLAIMIYYGHEGGALEKDAVLDFISQLDQTVFTAML
                     YKPLNQVNTPPFLVMVERLKSSSN"
     gene            11868..12512
                     /locus_tag="U1099_000639"
     CDS             11868..12512
                     /locus_tag="U1099_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEM4866980.1"
                     /translation="MKTIILFFLGASIGSFLGLVIDRFPEQSIIAPSSHCNACKRRLK
                     AWDLIPVLSQLSTKSKCRYCKAKIPYWYLGLEFLAGLVVLLCHFQVLNLTETILILAG
                     LVLTIYDIKHQEYPFAVWLIFTFIALILSQLNWLFCGFLLLAYLTEKWQINIGSGDFL
                     YLASLALICGFTELLWIIQISSLLGLLVFAIFKPKSIPYVPLLFLSSIPIILCI"
     gene            12620..14080
                     /locus_tag="U1099_000640"
     CDS             12620..14080
                     /locus_tag="U1099_000640"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEM4866981.1"
                     /translation="MTLFKDDSLTLHTDLYQINMMQVYFDQNIHNKHAVFEVYFRSQP
                     FKNGYAVFAGLERVVNYLNNLSFSQTDIDYLTELGYPQDFLDYLSQLEMTLTVRSAKE
                     GDLVYANEPILQIEGPLAQCQLIETALLNIVNYQTLIATKARRIKAVIEDEPLMEFGT
                     RRAQEMDAAIWGTRAAFIGGASATSNVRAGKIFGIPVAGTHAHALVQTYGDDYKAFKA
                     YAETHHNCVFLVDTYDTLRIGVPAAIKVAKEMGDKINFKGVRIDSGDMAYISKKVRQQ
                     LDDAGFTDAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVS
                     IEDEDGNMRDTIKLSSNAEKVSTPGKKQVWRITSKEKGKSEGDYITFADTDVTQMDNI
                     YMFHPTYTYINKTIENFDARPLLVPIFEKGKQVYELPSLAEIQTYANQEFDKLWDEYK
                     RVLNPQLYPVDLAQDVWDNKMNLINRIRKQTQTKSI"
     gene            14093..14917
                     /gene="nadE"
                     /locus_tag="U1099_000641"
     CDS             14093..14917
                     /gene="nadE"
                     /locus_tag="U1099_000641"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEM4866982.1"
                     /translation="MTLQETIIKELGVKPSIDPKEEIRRSIDFLKDYLKKHPFLKTYV
                     LGISGGQDSTLAGRLAQLTMEEMRAETGDDSYQFIAIRLPYGVQADESDAQAALAFIQ
                     PDVSLTINIKESTDAMVAAVNANGLAMSDFNKGNAKARMRMIAQYAIAGERKGAVIGT
                     DHAAENITGFFTKHGDGGADILPLFRLNKRQGKQLLAALGADEKLYLKVPTADLEEDK
                     PGLADEVALGVTYNQIDDYLEGKTIDPQSQTIIEGWWNKTAHKRHLPITIFDDFWK"
     gene            complement(15791..16318)
                     /locus_tag="U1099_000642"
     CDS             complement(15791..16318)
                     /locus_tag="U1099_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM4866983.1"
                     /translation="MRKFFQADGNLTMLGRQICKTLAGAYALAIVLLCFLPQTWYPQY
                     KDFSTPGIIQIGRLYLLPTPFNSIVNGDKVDSLADLGWIFLQNITNIFLLFPLIFLLL
                     FLREEWRSLRAVIRYSFCISLFIECTQLLLDLLIDAQRVFEIDDLWTNTMGGILAYFT
                     YSLIMRGIILSKQES"
     gene            complement(16315..17427)
                     /gene="rlmN"
                     /locus_tag="U1099_000643"
     CDS             complement(16315..17427)
                     /gene="rlmN"
                     /locus_tag="U1099_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEM4866984.1"
                     /translation="MKPSIYAFSQANLVDWILENGEKKFRATQIWEWLYRSRVQSFAE
                     MTNLPKSLIEKLEEHFVVNPLKQRIVQESKDGTIKYLFELPDGMLIETVLMHQHYGLS
                     VCVTTQVGCNIGCTFCASGLIPKQRDLTSGEIVAQIMLVQKYLDERNQNERVSHIVVM
                     GIGEPLDNYDNVMTFLRVVNDDKGLAIGARHITVSTSGLAPKIREFACEGVQVNLAVS
                     LHAPNNDLRSSIMRINRKFPIEVLFEAIEDYIKVTNRRVTFEYIMLNEVNDGVEQAQE
                     LADLTKNIRKLSYINLIPYNPVSEHDQYSRSTKERTLAFFDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSNTLKKDREKARARIAAAKAKAGIRA"
     gene            complement(17436..18077)
                     /locus_tag="U1099_000644"
     CDS             complement(17436..18077)
                     /locus_tag="U1099_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEM4866985.1"
                     /translation="MKKEISPEMYNYNKYPGPSFARVGDKVVSENIELDLLEDYKNAF
                     DQTIFGQRFSQLMLKFDYIVGDWGNEQLRLRGFYTDDKNVKSDLKISRLDDYLTEYCN
                     FGCAYFVLANPNPQDLPAEEEDRPRRKRSRSRNRNRNQQRTELIEPKENKSNRNDRQK
                     KGKQQNKQSEQRHFTMKNAGVKASQTERSQKHERKQSKREMNEVKRNFVIRQK"
     gene            complement(18265..19314)
                     /locus_tag="U1099_000645"
     CDS             complement(18265..19314)
                     /locus_tag="U1099_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEM4866986.1"
                     /translation="MKKNRKLTLIASIVLGTLLIWFAVFVRLPYYLESPGGAADIRQV
                     LTVNNQIDQEDGSYNFTYVSVQQATALQLFAAQFDPYTTVRSSEEMTGGADNEEYFRI
                     ARFYMETSQNMAKYQGLTLAGKEVNLDFFGVYVLALTDDSTFKKVLNIADTVVSINGK
                     TFESSPDLVKYVSGLEVGSDVTVGYISAGQEKSADGKIIKLSNGKNGIGITLVDHTEV
                     QSSVPIDFQTGDIGGPSAGLMFTLAIYTQLAEPDLRDGRIIAGTGTIEQDGKVGDIGG
                     ADKKVISAAKSGASIFFVPNNPVDVEVLKKNPKAKTNYEEAKEAAEKAGLDIEVVPVK
                     TVQEAIDYLKKTKGE"
     gene            complement(19301..19789)
                     /gene="coaD"
                     /locus_tag="U1099_000646"
     CDS             complement(19301..19789)
                     /gene="coaD"
                     /locus_tag="U1099_000646"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEM4866987.1"
                     /translation="MSDKIGLFTGSFDPITNGHLDLIERASGLFDKLYVGVFTNPKKA
                     GLLTGLERKAILEKLFVGMENIEVVLSENELVVDVAKRYGVTHLVRGLRNATDLEYES
                     SFDFYNRQLAPDLETIYLIAKPELKFVSSSQVRELLYFKQDIGPYVPEIVSEEIRKNE
                     EK"
     gene            complement(19779..>20196)
                     /gene="rsmD"
                     /locus_tag="U1099_000647"
     CDS             complement(19779..>20196)
                     /gene="rsmD"
                     /locus_tag="U1099_000647"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEM4866988.1"
                     /translation="GGRVLDLYAGSGGLSIEAISRGMESAVLVERDRRAQAIIRENIA
                     MTKEADKFQLLAMEARQALNSVGGPFDLVFLDPPYAEQEIEDVITELCRRDLLADDVM
                     VVCETDKAVSLPEEIAELGIWKEKLYGISKVTVYVR"
BASE COUNT         5844 a         4589 c         3638 g         6125 t
ORIGIN      
        1 tctttgggtt ctttaacaat ttcaacatta tcttcaagag tatctaccaa tgtcacttta
       61 gtcagtacat ctttaaattt ttctgcaaca ttctcatcaa cacttaaaaa cttcttatca
      121 tccccagaca tttttttcaa gtattcatca aatttatttt tattcttttc ttcgccagct
      181 ttgtctttgg tcacatcaac agcgtagaca tgaataccat ttttagtagc aatcttggcc
      241 tctgcttgag cagagaggtt gtggttgtta aaaatatttt ttctaccgta gaaaatataa
      301 tcaacgaggg aattcgcaaa taaatcaatt gctttttcag aagtattcgt taaaccgagc
      361 ttttcagcga tatagctaag agtgtttgta ggtaactcag ctttttttaa ctcagttgtt
      421 aaattatgtt ttatcttgct aacaatttga tttctaaatg tcaacggctc aactttacgt
      481 tcataaaagc tacggaaatg ataaccttca ccaatttgtt tctcgtaatt gaaatttttt
      541 tcttctaact gcgaactgtc tacatcaacc aaattaatat agtctaacgg atcttttgca
      601 attattccta taagctttgt tattcctgct atagtggata catcatctaa agctttatcg
      661 aaacgtgtaa tcccaacttt gttcaaaaaa cttattagac tctttgcatc atccaacaag
      721 tttgccctgc gaacagtcac atcaaatgta aatggccaag gcaagagtgg atttgtatgt
      781 acaaccttat tttcctcaat ggtcttataa gtaggttttg attttacatc atccgacaaa
      841 tcataactaa atgtagactc cccttgtgac agaagtacaa tatgtttgcg tttccccgag
      901 ttctcgcttt ctaaaatgcg acgtgcttca tggattgctc cctgcatatc tacgccccaa
      961 ttgtaatttt gttcagcatc agtgaaaagc ttctcaaccg tagtttctac attagcactg
     1021 gtatggtaac ttcttgagct tcagataact tgcggttgaa accgattaca cgcacagagt
     1081 tacgagaatt atgatcattg gttgggtcag tcatcttttc gacaagcttt ataatgttat
     1141 ctttagctgt tttttagcat cttcatccat ttttttatat gtatccacca agacaactat
     1201 ttctgcaccc ttgtcaattt ctttcggcac aatttgagtt gttactgtga caactccctc
     1261 ttgatctact tctgctgttc gagaaactgt aaaaagctct ttagaagttt tgtccactgt
     1321 actagatact gtttccgttg gtttttcaac cttagtagaa tagtcgtcta cttccttctc
     1381 ctcatactga acggttcgtt cttctttttc tgttgctttt tcttcagaag aactagtgtt
     1441 ttcagcaact tgcacttctg attctgaagc gttttcattc gtcaatgcct caggagaagc
     1501 agattctgca ttcgattttg gtaagtccaa agtagaagag gagtcaacca cctctccttc
     1561 cttgttttca ttagcttggg caacttcagg gtcttcaacg acagcagtag acctaatcag
     1621 agaagtgcta tctgtctccg aagttttttc taaagtaggt gtttctgcat atactgtcac
     1681 agtatttttg ggatagagtg ttcccgataa agctacccct gctcctagcg aaacaagtcc
     1741 aattgatagc ttccgcaaag tgaatttagg atgattcatt atgatacctt ctccttgttc
     1801 tctataaatt tagaatgtat tttcaccctt ctttaggtat tttcaaaaaa ggaagtatgg
     1861 atgtcaatgg taaataactt tagtataatt ttgttcaaca agcgtagagc ttttggaaaa
     1921 agaatgggca ccttgtgtat tcataaggtg cctaagtcct tctgttaact gcttacttca
     1981 cctatcaagt caagcaagca tgattacaac tcaaagttcc tatttatcat taacaatgac
     2041 catatcacaa aatgtgcgag tctattacta cataactcat tctaccatat aatttaaaaa
     2101 cagaatttgt tttttctcta tcgtttgttt atgtttattc tattacaaaa atgataattg
     2161 gtctttgagt ctgttatcac acacatttat acaagtgata aatattgcaa tgagtcttca
     2221 aactctactt tcacctctat aagcaaataa ttcattgaaa gaaaaatttt aactctttta
     2281 atttaaagct acacaatgcc tctcatctgt cgcaaacgaa tttttgagag agctttgtca
     2341 tcgcctttta ctcactttct gttatctttt tcttgccaat gaataaagca aataagaata
     2401 atatgatcga taacattata ttggcaacca agctcgtttt ttcaccggtc ttaggcaggc
     2461 tttgaacttg ggtattctca ccactatcat gcacagatac gtttcctttt gtccctttgt
     2521 ctcttacagt acgagccatt ttctcttgct tagtaggcat atcttttttc gaatcatcaa
     2581 aaatagaaac cgtttcttct tttgatggct ctgcattttg ctctactttt ttgtcagtca
     2641 gcgtttggcg tacaggtggc aaagtagttt gctcaagtgt gtctgattgt tcgatatttt
     2701 tggtaatttc tttgctaggt tgctcttcct tctcgtcctt tttatctggt ttattaatgc
     2761 ggtgaccatt tcctttttga accgttacga cttcccaagc tacctgttca ccttctttat
     2821 caaacgctct aaacgataag gtttctccat tttttaaaac acgactaccc aaatcaaatt
     2881 cgaatcgacc tgttgaatca gttgaagttt taccaattat ctcctcgcca ttctgagttg
     2941 atactagttc aatcattaca tctggagctg ttttgccact gatgtaacca tgatctgtag
     3001 tgactttttc tacttgaaat ggtaaatcca tcacgtcttc atcttccaaa tattgcccta
     3061 caacttcttc cttactagcg atgatattat ttgtaaccgg gtcaatgatt tcaaagcgaa
     3121 ttctctcacc tttttcaaaa ggcaattgta aatcttgaat ttggaaacca aaaaatccta
     3181 attcgtccac ttgaactgga ggatactgtt ttcctttgat tgacgaaacc atgcgaacgt
     3241 aggcattaag atgtatcgtt cgaccgacaa tataatcttt tttattatgc agtttaggta
     3301 caatgatagg atcttcatct gtcgcttcat ccatatctgc atcttgtaaa taagttgcta
     3361 aagatgccat aacagattta tccaataata tacttgaacc atcaactgta tcaagatgga
     3421 cagataccaa gtcctcttgt tgattgtgag tcaatctgta gtacttatcc tcggattgta
     3481 gggcaggcaa agcaatctta tagtgacctg tctcatcagc aatagcttcc atgataaact
     3541 ctgaagtatc agcattgtat agatgcacac gacctaaagg aaatgtagtt ccttccaaaa
     3601 ctgaagtcgc acttgataaa ggttttactg tcatttgtga agatactttt tcttttacca
     3661 atggttttac aatagtatta gtaatcaccg gctgaccatc ttctgataca aaagacaaaa
     3721 ccaatctatc atctgcttta aattttgaac ctgctaaact ctcggaaaaa gtaacctcaa
     3781 accgtccatc tttaggatca attagtgtct tatcagagac aaaactacca tttattgata
     3841 gatagactga acctttgaca gaggtatgac caataacttt gctcgtatgt tcaaaaactg
     3901 gttcgatgaa cacttgatgc attccattag atgttttttc taatcttttt gctggaattt
     3961 cataagcctc tggatgtcga ttagtcctca ctactacctt acgctcttca gaatctgcct
     4021 ctagagtcca aaactgtttc ggcaaagcac taatttccac agtttggctg tgagcaagtg
     4081 gtttactcaa ccgataagaa aaatctccct tatcatctgc ttccaaaata ttctcaatag
     4141 aagtaattgc ttccccatct atcgtaatcg ctacatatcc accaggagtg gtattccctg
     4201 agatggtttg atctccagct tgaatcggag caacctctag atggttttca ataaatggaa
     4261 gttcttcctc catatcctcc atctcttccg attttttttc ttcactcggt acctcatcag
     4321 tcacttcctc agcttcagca ttttccgaaa ctgtattggt aagcgatgat tccttgcttt
     4381 ctaattgagt ttcaagagct gtagcgtcag tctcctcagc cattgcggtt tccatattca
     4441 tccctgtacc aatccataga aaagcaattg ccaccgaaac taaacccatc ttactttttc
     4501 tcaacgaaaa aatcttgcta ttcatctttt ctccttatat ctgtattaat aattaacgac
     4561 acatattgtc ctagttttat tataaaaaaa acatctcata gaacattgtt tttcattagt
     4621 tttttatctg ttttacagtt gcctgtaata tatagtatgc tacatttcgc aaaaactttg
     4681 ctggttctca tcgtcattta gtttttcttt tattacttct gtaatcaatt tgaattgaca
     4741 gaacgattag aagcacttta cttgtaaaca aaatttctaa attatggcac attcaaagta
     4801 aaaaactagc gcctgaagtt ttctatgata cagatacttg tttatctcgg agtgctcctt
     4861 taattcgtca acttatactc aatgaaaatc aaaatcaaac taggcgacgc agatacagat
     4921 agaactgaag ttcatcaaat caagttaaca acgtctgatt ttcgaagagt attaaataag
     4981 agtgggttta tttgtcatga tgccacttgt ttattttgtt aactatcact tcataaaaca
     5041 tgattgtcct cataataaaa agcacccgaa ggtgctagat aaggccttcc taccgacttc
     5101 acgttcgagg tcggtagaaa gcttcattag gcgttgaaag catctgtcaa ttgtggcacc
     5161 acttgtttct tacgggaaac ggcacctgca aggaaagcgt ggttgttttc caacttgaag
     5221 ttgaaggccg cttccacctt gtccatgttg cttcctaggg caaggatttc agagtttgag
     5281 ttgacgatgt ccgtaatcat gaggacaaag tcagagtagc catttgctgc ggaagctgct
     5341 gtcatagtgg cttcaatctc tgcttggcgt tccaaaactt ccgcaatatc aacagtgttg
     5401 acctgagcta cacggacgtc attgccattc aaaccaaatg ttttggcatc aatgtcaatc
     5461 aattcttctg ctgacttgct ggcaaggttt gtaccagctt tcaagagtgt caaaccgtat
     5521 tcttccaagt tcacaccagc aatttcagcc aattcagctg ctacttgtgg atctgttgta
     5581 tgagtggttg gcgatttgag caagagagta tctgaaatca aacctgatag gagaagtcca
     5641 gctacttctt ttggaggtgt tactccgttt tctttaaagg cccggtaaac gattgatgat
     5701 gctgaaccta ctggttccaa acgcatgtac aatggatttg ccgtttcaaa gtttgcgaca
     5761 cggtggtggt caataacagc tgccacttcc acttccttga tgtctgcgat tgattgttgg
     5821 aattcattgt ggtctgtcaa gatgacttgg ctaacgcctt ctgcttttgc agactcaacc
     5881 acgcgcggtg cagtaacacc aaaatagtca agcgcaaacg ctgtttcttc atttggcgtt
     5941 ccaagtgcaa ctgcttccgc atcacgacca aacacttcac gctccaagtg agcccagccg
     6001 tacgatgatg caatggcatc tgtatcagga ttttggtgac caaaaactaa aaatttagac
     6061 atgtctacaa cctcattttt ctaataatct cctacatttt accataaata gaaagacttg
     6121 tgaacatgga cgggtcggga tttgccaaac gttttcaaaa agagtaagat agaaggtagc
     6181 aatacaaaag gagaaacata tgaaattttc agactatacc tatgtccgtc cagattatga
     6241 aactatcaag gctcaatttt ctgacctgac agaccagttg gctcaggcaa gtgacttgga
     6301 aacaactcgc acacttgtgg cagccgttac caaattgctc aacttggttg atacccagta
     6361 caacctctgg atgatccgtc atactatcga catgaacgat gagttctaca atgaagaaac
     6421 caagttttgg aatgaatatt cgccactttt tgaagaattg accaccaact actaccgtgt
     6481 cattgtccag acaccttaca aggctgagct tgcagacatt ttaccagaaa ccttcttcat
     6541 gcaggctgag aacaagctca agaccttctc atctgatgtt atccccttgt tccaaaagga
     6601 aaatgaattg accgatgagt acagcaaact gattgctgga gcagagattg atttccaagg
     6661 tcagacctac aacctggcac aaatgggacc attcggtcaa tcaaccgacc gcgaggtccg
     6721 caaagctgct tctgctgcca caactgcatt ctttgaaagc aaagaagctg actttgaccg
     6781 tgtttacgat gaattggtca aggtccgcac ggaaatcgcc cacaaacttg gcttcaagga
     6841 ctatgtggaa tacggctacc tcaagatgaa ccgtttcgat tacaattgtg acatggtcaa
     6901 ggtttaccgt gaagaaatcc tcaagcatat cgtaccgatt gtgcaaaatc ttcgtgaacg
     6961 ccaagccaaa cgcttgcagg taccaagcct gaaacactac gacctcaatc ttgagttctt
     7021 agacggaaac gctgtgccac aaggtgaccc agactttatc ctcagccagg cccaagacat
     7081 gtaccgtgaa ttgtccgcag aaacaggcga gttcttcgat ttcatgattg agcatgaact
     7141 cttggacttg gttgcaaaac caggtaaaaa cagcggtggc tactgtacct atattcctga
     7201 cttcaagagc ccattcatct tctccaactt caacggaacc agcggtgata ttgatgtctt
     7261 gacccacgaa gcaggtcacg ccttccaagt ctatcgttca cgttggattc aaagtccaga
     7321 agttgtttgg ccaacctacg aaacctgtga aatccactcc atgtctatgg aatttatgac
     7381 gtggccatgg atggaccgtt tcttcaagga acaggtggat aagtacaagt ttacccactt
     7441 ggcaagtgcc cttctcttct tgccttacgg tgtcttggtg gatcacttcc agcacgaagt
     7501 ctatgaacat ccagagatga caccagctga acgcaaggcg acttggaaaa aactccaaga
     7561 cctctacttg ccagaccgtg attattctga gtcagaagcc ctcaaccgcg gcatcttctg
     7621 gtatcgtcaa ggtcatatct ttgctagtcc attctactac atcgactata ccttggctca
     7681 agtttgtgcc cttcaattct ggaaacgtac acaggtagat cacgatgaaa atgcttggga
     7741 agactatatc cgcatctgtg atttgggcgg caccaagtcc ttcttgcaag tggttgaagc
     7801 cgcaaacctc caatcaccgt tcaaagaagg tgcccttgaa agcacagcca aggctgctgc
     7861 cgattggtta aatgcagtta aggatagcga cttataaaac acaaaaatct gaatgaatca
     7921 aatgactcat tcagattttt tgattatatg atacttgtcc ctaacacatt aaatcttttt
     7981 cgcagcaatc tcagacctct cactattatc ccttaattct attcaagtac tcctgatagg
     8041 tctctgtctc catcaaggcc tttgcatttt ccactcgagc cttcgttggg ggcttggtcc
     8101 cctctagtgg atagggtctg cccaactcac gccacttaaa ttcaccaaga ttgtgataag
     8161 gtaacacctc aaaacgttcg acatttttta atgttttaac aaacttacca agttcaatca
     8221 agtcctcatc tcggtcagtc aaattcggca ccaggacatg acgaatccaa acaggcttac
     8281 caatgtctga tagatattga gcacattcta aaatagtctt gttactatga cctgtcacaa
     8341 tgcggtgttg ctcaggattc atttccttaa tatcaaggag caccaaatct gttacttcca
     8401 taagccgact atatttttcc aagtaacgct ctgtcttacg gaaagtcaac gcacaggtat
     8461 ccaaagtagt gtgaatccct ttttcttttg caagggtaaa cagagcaatt aaaaaatcaa
     8521 tctggatggt tgcttcacca cctgaaacgg taataccccc ttcttttccc caaaacatac
     8581 gaaaacgcag agcttcattc aagacatctt ctgccgttct ctcagtcgct gctggattga
     8641 ccagatccca agtatctggg ttatggcaat actggcaacg catgtgacag ccctgcataa
     8701 aaactacaaa acgtacccct gggccatcaa ccgaaccaaa actctctgtt gaatgaacca
     8761 aacctgtcac tttacgataa tcaatttctg tcatcatctc atcctcatca ttttataaga
     8821 ctagtatatc acaaaaatga aaacgcttct atcatttact ttctttcttt cataaccatc
     8881 atcttccgca agaaaaaagc taaaagacta ggttgcccta gtccttatcg gtttcttctt
     8941 tctccagtgg tgtaatcaag agttttaatt ttgttacgcg accatttttt accttatcgt
     9001 taataagaac taggtggtga tctttactat ctacttcaaa ggaaactttc tcatcttggc
     9061 tcggaatggt accgactcct gtcagataat aaccagcaat cgtatcaacg ttatcacttt
     9121 ccaactccat ctcaaaatgc tcattgaagt cattcaaggt catatttcct tggacgatat
     9181 aggtgttgtc agcaatttct cgaacaaaaa tttctgtctt atcggtctca tcatcgattt
     9241 ccccaacaat ttcttccagc aaatcttcca aggtaacgag acctgcaact ccaccatact
     9301 catctaacaa gatggccatt tgattttggg tatttctcaa tgatgtcaac aaatcatcca
     9361 cgaaaatcgt ttctggaacg aagagaggct cctgcataat gcggcggatg ttgagattgt
     9421 caaagccatt actgaaagct tctgacaaga ttttcttggt atgaatcaga ccaatgatat
     9481 tgtccttatc gccatcataa acagggatac gtgaaaaatt ctgcttgagg atagctgcca
     9541 tgatggttgc tgtatcatcc tcaatatcca ccatgaaagc atccgtacgg ggaaccatta
     9601 cttcacgtgc catcagctca tctaaagaga acacaccttg aagcatttcg atttcttcgg
     9661 catctaaggt tttctcactc tttgtcaaaa tatattcaat ttcatcccgt gtcatttgct
     9721 catccgcatc atcaaaattc ataggagtaa tacggctgat aagattcgtt gcagctgata
     9781 ataaccacac gaacggacta acaatttttc caaggaaaat aatcactggt gcagtgacta
     9841 ctgccagatt ttccttcata ttcatggcaa tccgcttagg gtacaattcg ccaagaacga
     9901 ttgaaacgaa ggtcaacata gctagtgcaa gccagtatcc tgcggtttgc gctgttgttg
     9961 aattccccat ccatttggca aatacagctc ccaaatcatt agccaagctg gcacctgaaa
    10021 ggatggagat gaaggtaata ccgacctgaa tggttgatag aaaattgtta ggattctcta
    10081 aaacttgaag caagcgaata tatttcctct caccgtctgc agccttttgt tctactcgag
    10141 aacgattgag ggatacaagg gccatttctg aggctgaaaa gaaggcattg aacagagtca
    10201 ataagaataa aattaacagt tgtaaataga tggtctgact accagggtct tccatgatgt
    10261 tctcctataa taaaatttat aacatttcca ttataccaca aattctaaaa tttaacatat
    10321 ttcttgctat aattagtccc tcatgtctgt ttatgttaca ataatcaaaa ggaataggag
    10381 agcaactatg attatttcga tgaagaatgt ccactataaa cgacaaggta aaactatgtt
    10441 agaagacatc aactgggaat ttaaaaaagg cgagcgttgg gctattttag gactcaacgg
    10501 ggctggaaag tcaactctac ttcgcatttt aatggctgaa ttttggaaga cctctggtga
    10561 cctgactgtt cttggagtgg agtttggtaa gggagatatt cccagcctac gcaccaagat
    10621 tggggtagtt ggctcctttt tggcagagcg ttttccaatc gatttgacgg ctgagcagat
    10681 tgtcttgaca ggaaaatata aatcctccat cctctatcgt gaatacggtc aagctgaatt
    10741 ggatgaagcc atagacatgc tcaaaaccat taaggctgag cacttgatcg gtcgggccta
    10801 tgctagtttg tcacaaggag aacgccaact cctgctgatt gcccgaagtt tgatggaaga
    10861 gccggctatt ttgattttag acgaagcaac tgtcggtttg gacctcctag ctcgcgagcg
    10921 cctgctccac cacattgact acctctgtca actcccaacg gctcctgcca ttatctacgt
    10981 cacccatcac gccgaggaaa tcactgataa tttcacccat gtcctcctgc tcaaagatgg
    11041 catggtcttg gcacagggac ccaaagaaga tatcctgaca cctgaactcc tcagcgattt
    11101 ttatggaaat caggtggaat tgattgactt gggtgaggga agattgttca ttaagccgat
    11161 attataaaaa acaaatccag ctctagttcg agctggattt taatcgttcc accatgacca
    11221 aaaatggtgg ggtattaact tggttgagcg gtttgtagag catggctgta aagacggttt
    11281 ggtcaagctg gctgataaag tctaaaactg catccttctc aagagctcca ccctcgtgac
    11341 catagtaaat catgatagcc aagcgacctc caaccaccaa tctattcagg actttctcaa
    11401 tggcttgaag ggtggttgca ggcagggtaa tgactgactt gtccgccgaa ggtagatagc
    11461 ccagattgaa aatcgctgcc ttgcattcat ccacatactg gtctaaattt tcgtgtcctg
    11521 tcaaaaccaa ttgggcattg gttaagccag ctttttctag tttttcagcc gttgtggtca
    11581 gggcttgttc ctgaatatca aaggccacaa cttttcctgc tcgttgggct aaaaatagcg
    11641 tgtcatggcc atttcccata gtcgcatcga ccgctaggtc ctggtcagtt aaaatctcat
    11701 ctaaaaaggc atgggccatg tgtaaaggtc taagcatact ttctcctaat tactgtactt
    11761 tctactattt tgtaaaattt atcacaccta gtatagcata ctttccatct tcaaaaaatt
    11821 ttttaaaatc tactcatatt cctccatcca tttacgaata aataggtatg aagacaatta
    11881 tcctattttt ccttggagct tctatcggct ccttcttggg attggtcatc gaccgtttcc
    11941 ctgaacagtc cattatcgcc ccctctagtc actgcaatgc ctgcaaacga cggctcaagg
    12001 catgggactt aattccagtc ctatcccagc tttcgacaaa atccaaatgc cgttactgca
    12061 aggcgaagat accttattgg tatctgggac tagaattctt agctggtcta gttgtcctgc
    12121 tctgccattt tcaagtccta aacctaaccg aaaccattct catcttggca ggactagttt
    12181 tgaccattta cgacatcaaa catcaggaat atccttttgc tgtctggctc atttttactt
    12241 ttattgctct gatactctcc cagctcaact ggctcttctg tggcttttta ctcttggcct
    12301 atctgactga aaaatggcaa atcaatattg gttctggtga ctttctctat ctggcaagtt
    12361 tggccttaat atgtggattt acagaactcc tctggattat ccagattagt tccctcctag
    12421 ggcttcttgt cttcgccatt ttcaaaccca agtctattcc ctacgtacca ctcctttttc
    12481 tttcaagtat tcctatcatt ctgtgcatct aaatcgagtt tttctcgatt tttttgttat
    12541 aatagaaggc atattagagt tcaactccat caaaataatt ggagtgcctt cttgatcgga
    12601 aggcaaaagg agaaacgtta tgacactatt taaggatgac agtttaaccc tgcataccga
    12661 cctttatcaa atcaacatga tgcaggtcta ttttgaccaa aacatccaca ataaacacgc
    12721 tgtattcgaa gtctatttcc gttcacaacc ttttaaaaat ggctacgctg tatttgccgg
    12781 tttggaacgc gttgtaaact acctcaacaa tctcagcttt agccagacag acattgacta
    12841 tctgacagaa ctaggttatc cgcaggattt ccttgattac ttgtcccaat tggaaatgac
    12901 tctcactgtc cgttcggcta aggagggcga cctagtctac gctaacgaac ctattcttca
    12961 aatcgaagga ccattggcac aatgccaact aatcgaaact gccctcttaa acatcgtcaa
    13021 ctaccaaacc ctgattgcca caaaagctcg ccgtatcaag gctgtcattg aagatgagcc
    13081 cttgatggaa tttggcactc gccgtgccca agaaatggat gctgctatct gggggactcg
    13141 agcagccttt attggtggag catctgcgac ttccaatgtt cgagctggca aaatcttcgg
    13201 catccctgtc gctggtaccc atgcccacgc cttggttcaa acatacggag atgattacaa
    13261 ggctttcaaa gcctacgctg aaacccatca taactgtgtc ttcctcgttg atacctatga
    13321 tacgcttcgt attggtgttc cagcagccat caaggttgcc aaagaaatgg gagataagat
    13381 taacttcaaa ggggttcgga ttgattcagg cgatatggcc tatatttcca agaaagttcg
    13441 tcagcaatta gatgatgcag gttttacaga tgcgaagatt tacgcatcta atgacctgga
    13501 tgaaaatacc atcctcaacc tcaaaatgca aaaggcaaaa attgatgtct ggggagttgg
    13561 taccaaactc atcaccgcct acgaccaacc agctctgggc gctgtctaca agattgtatc
    13621 catcgaagat gaagatggaa atatgcgtga caccatcaag ctgtcttcta atgcggaaaa
    13681 agtctccact cctggtaaga aacaggtttg gcgcatcacc tccaaagaaa aaggaaaatc
    13741 agaaggggac tacatcacct ttgcggacac tgacgtcaca cagatggata atatctatat
    13801 gttccatcca acttacacct atatcaataa aaccatcgag aactttgatg cccgtcctct
    13861 cctagttcct attttcgaaa aaggaaaaca ggtctatgaa cttccaagcc tagcggaaat
    13921 tcaaacctat gccaatcaag aatttgataa actctgggat gaatacaaac gcgtgctcaa
    13981 cccacagctc tatccagtcg acttggcaca agatgtttgg gataataaga tgaacctcat
    14041 caatcgtatt cgcaaacaaa ctcagaccaa atccatctaa gaaaggtatg ttatgaccct
    14101 acaggaaaca atcatcaaag aattaggcgt caagccaagc attgacccca aagaagaaat
    14161 ccgtcgctcc atcgactttc tcaaagacta tctgaaaaaa cacccatttt tgaaaaccta
    14221 cgtcttgggg atttctggtg gacaagattc gaccttggct ggtcgtttgg cccagctgac
    14281 tatggaagaa atgcgggcgg aaacaggtga cgattcttat caatttatcg ctatccgcct
    14341 gccttacggt gtgcaagctg atgagtctga cgcccaagca gccctagcct ttattcaacc
    14401 agatgtcagc ttaaccatca acatcaagga atcaacagat gccatggtgg ctgcagtcaa
    14461 tgcaaatggg cttgctatgt ctgattttaa taagggaaat gctaaagccc gcatgcgcat
    14521 gattgcccaa tacgctatcg ctggagaacg caagggggct gttattggaa ccgatcatgc
    14581 cgctgaaaat atcactggtt tctttaccaa gcacggtgat ggaggtgcag atatcctgcc
    14641 cctcttccgc cttaacaagc gtcaaggtaa gcaacttcta gctgctttgg gagcagatga
    14701 aaaactctac ctcaaagttc ctactgcaga cttggaagaa gacaaaccag gccttgctga
    14761 cgaagtcgca cttggtgtta cctataacca aatcgacgac tatctcgaag gtaaaaccat
    14821 cgaccctcaa tcccaaacca ttatcgaagg atggtggaac aaaacagccc ataaacgcca
    14881 cctgccaatt actatttttg atgatttttg gaaataatac tcctcgaaaa tcaaaatcag
    14941 acgttattga cttgatttga tgactgaaaa gctcctgtgg aggttttcag cctgttccct
    15001 tgaaatataa aaggaacaga gttctaacat caacttgaag gcgcaaacaa tgttcgctat
    15061 atttccaacc ttcaactatc tcccagatag ttgaagctag gctacttttg attttcatcg
    15121 aataataaaa aatggagccc tgacagttga accgtcaggg gttttgtttt ttattataac
    15181 ttcaaaccat aatgaaaaaa gcccacgcag gaaaaaatat cctgcgtggg cgtcaatgga
    15241 cacggtgcca cctcactatg gactcgacta gaaaagtcca taactttttc tcctctaaca
    15301 aggaggcgca cgataagggg tgcggaccga actctcattg tttcgaattc atcgaaccaa
    15361 actcacttta ctaaaccaga caatccataa aatggatacg aatgcttgtt tggcatcagc
    15421 ctatctcata aacctctatc atctattcac accaaccata gactctctaa agacaaagtg
    15481 tttattacat tttctgatta cttgtaatta tacgttacaa aatcaaaatg gtcaagtgat
    15541 ttttagaaat tttctaaaca atttgaaaat tcagtttcca ttgcctgaat ccattaaaaa
    15601 ctaacaaaaa gccactctgc ttaagtgact ttctaaaaaa ctgtattcac gagcgaagca
    15661 ctcatatcaa agatagcttt agctagtcaa ggtcattttt aagggaatac tgactgtatt
    15721 tcaaaaaaat aacaatgtgt agcataaaca atagccttag atggacattc tgaacctgtg
    15781 acggtaggtt ctaagactcc tgtttggaaa ggattatccc tctcattatc aatgaatagg
    15841 taaaataggc tagtattccc cccattgtat tggtccataa atcatcgatt tcaaagacgc
    15901 gttgggcatc aatcagcaaa tctagtagta attgtgtgca ttcaataaaa agactgatac
    15961 aaaaactata acgaatcacc gctcgaaggc tccgccattc ttccctaaga aacaaaagaa
    16021 gaaaaatcag agggaaaagt agaaaaatat tagtgatgtt ttgcaagaaa atccacccta
    16081 ggtccgccaa actatctacc ttgtccccgt tgacgatgct gttgaaagga gttggtaaaa
    16141 gatagagacg gccaatttga ataattcccg gggtcgagaa atccttatac tgtgggtacc
    16201 aagtctgagg tagaaaacaa agtaaaacaa tagccagagc ataggctcca gctaaggtct
    16261 tacaaatctg tcttcctagc atggtcaagt ttccatctgc ttggaagaat tttctcatgc
    16321 tcgaatacct gcctttgctt ttgcggctgc aatacgagca cgagcttttt cacggtcttt
    16381 cttgagcgta ttggaacgca attgaccaca ggcagcatca atatctgtac catgttcctg
    16441 acgaacgaca cagttaacac catttttctt caaaacatca aagaaagcaa gagtacgttc
    16501 cttggttgaa cgactgtact ggtcatgttc actaactggg ttataaggaa tcaagttgat
    16561 ataagacaat ttacgaatgt tcttggtcaa atcagctagt tcctgcgcct gttccactcc
    16621 atcgttgacc tcattgagca taatatactc aaaagtcaca cggcggttgg tcactttgat
    16681 atagtcttcg attgcttcaa aaagaacttc tatagggaat ttgcggttaa tgcgcataat
    16741 gctcgaacga aggtcattat ttggcgcatg tagagatact gccaagttaa cttggacacc
    16801 ttcacatgca aactcacgaa ttttcggtgc taaacctgac gtagaaacag tgatgtgacg
    16861 ggcaccaatt gccaagccct tgtcgtcatt aaccactcgc aagaaggtca taacattatc
    16921 gtagttatca agcggctcac caatccccat cacaacgata tggctaacac gctcattctg
    16981 attgcgctca tccaagtact tttgtaccaa cataatctga gccacaatct caccgctagt
    17041 caagtcacgt tgcttaggaa tcaatccaga tgcacagaat gtacagccga tattacagcc
    17101 aacttgtgtc gttacacaga ctgaaaggcc atagtgctgg tgcatgagaa ctgtttcaat
    17161 caacatgcca tctggcagtt caaaaaggta tttgatagta ccgtccttag actcctgtac
    17221 aatccgttgt ttgagcggat taactacaaa atgttcttcc aacttttcaa tcaaagattt
    17281 tggcaagttg gtcatttccg caaaagattg gacacgagaa cggtaaagcc attcccaaat
    17341 ttgagtggca cggaacttct tctctccgtt ttctaaaatc caatcaacca gattggcttg
    17401 actaaatgca taaattgatg gtttcatatt tctccctatt tctggcggat aacaaagtta
    17461 cgcttcactt cattcatctc acgcttactc tgcttacgct catgtttttg agaacgctcc
    17521 gtttgactcg ccttaacacc agcattcttc atagtaaaat gacgctgttc cgactgttta
    17581 ttttgctgct tccctttttt ctgacggtca ttgcgattac ttttgttttc ttttggctca
    17641 ataagctccg tccgttgctg atttctgttc cgattgcgag aacgactccg cttccgacgc
    17701 ggtctatcct cctcctcagc aggcaagtct tgtggattcg gattggctaa gacgaaataa
    17761 gcacaaccaa aattacaata ctccgtcaaa tagtcgtcca agcggctgat ttttaagtct
    17821 gatttaacat ttttatcgtc tgtatagaat ccccttaatc tcagctgctc attgccccaa
    17881 tctcccacga tataatcaaa tttgagcatg agttgtgaaa aacgttgacc aaaaatcgtc
    17941 tggtcaaaag cattcttgta atcttccaaa aggtccaatt caatattttc agatacaacc
    18001 ttatctccga cacgagcaaa actcggacca gggtatttat tatagttata catttcaggt
    18061 gaaatttctt ttttcatttg tcctcctatc ttataaaatc ttcatacgta aaaatcattt
    18121 gattaataat ctatctggtc agaatctaaa ttctcacact taaccttatc attataacac
    18181 gatttatctg gaaattcttc tactttacca tttaggagaa atttctgaca attataatat
    18241 tattctctag caaaaaagga cgatttactc gcccttcgtt ttcttcaaat aatcaatagc
    18301 ctcttgaaca gttttcacag gaactacctc aatatctaga cctgctttct cggctgcttc
    18361 cttagcttct tcatagttcg ttttagcctt tgggtttttc ttcaaaactt ctacatcaac
    18421 tggattgttt ggtacaaaaa aaatacttgc acctgactta gctgccgaaa taaccttctt
    18481 atcagcccca ccaatgtcac ctacttttcc atcctgctcg atagtccccg taccagcaat
    18541 aattcgacca tcacgtaagt caggctctgc gagttgagta tagatggcaa gtgtaaacat
    18601 cagaccagca cttggaccac caatatctcc cgtttggaaa tcaattggaa ccgaactttg
    18661 aacctctgta tggtctacga gagtgatgcc tattccgttt ttgccattag ataatttgat
    18721 aatcttaccg tccgcagatt tttcctgacc agcgctaata taaccaactg ttacatcact
    18781 tccaacctcc aagccactca catatttaac caaatcagga gaagactcaa atgtttttcc
    18841 attgatactc acaacagtgt cggcaatatt aagaactttt ttaaaggttg aatcatccgt
    18901 caaggcaagc acatagacac caaagaaatc aagattgact tcttttcctg ccaaggtcaa
    18961 tccttgatac ttcgccatat tttgcgaagt ttccatgtaa aaccgtgcta tacggaaata
    19021 ctcctcattg tcggcccctc ctgtcatctc ttcagaggaa cgaactgtcg tatagggatc
    19081 aaactgagca gcgaatagtt gtaaagctgt agcttgttgg actgatacat aggtgaagtt
    19141 gtaagagcca tcctcttggt ctatttgatt gttcaccgtt agtacctgac gaatatcggc
    19201 agctccacct ggactttcca gataataagg cagacgaaca aaaacggcaa accaaatcaa
    19261 aagagttcct aaaacaatag atgcaattaa ggttaacttc ctatttttct tcattctttc
    19321 taatctcctc actaacaatt tctggaacat aaggacctat gtcctgtttg aaatacagca
    19381 attctcgaac ctggctggat gatacgaact taagctctgg cttggcgatt aaataaatag
    19441 tttccaaatc tggcgccaac tgacgattgt aaaaatcaaa acttgactcg tattccaaat
    19501 cggtcgcatt acgcaatcct cggaccaaat gtgttactcc atagcgctta gctacatcga
    19561 ctaccaattc attttcagac agaacgacct cgatattctc cattcctaca aaaagcttct
    19621 ccaaaatggc ttttctctcc aaacctgtca agagtcctgc ctttttagga ttggtaaaaa
    19681 ctcccacata taatttatca aatagtccac tcgctcgttc aattaagtcc aagtgtccat
    19741 tggtaatggg gtcaaatgag cccgtaaaca atccaatctt atctgacata tactgttacc
    19801 ttactaattc cgtagagttt ctctttccat atccccagct ctgctatttc ctccggaaga
    19861 gagaccgcct tatccgtttc gcagactacc ataacatcat ctgctagtaa gtcacgccga
    19921 caaagttctg ttatcacatc ttcaatttcc tgctctgcat aaggagggtc caagaatacc
    19981 aggtcgaaag gacctcccac actgttcaaa gcctgtctag cttccattgc tagaagctga
    20041 aatttatctg cttccttagt cattgcaata ttttcacgga taatggcttg tgctcgtcga
    20101 tctcgctcaa ccagaactgc tgactccatg ccacgagata tagcctcgat agataaacca
    20161 ccactgccgg cgtacaagtc aagaactcta ccaccc
//
LOCUS       DASFSW010000023        19936 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.023, whole genome shotgun sequence.
ACCESSION   DASFSW010000023 DASFSW010000000
VERSION     DASFSW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19936
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.023"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            99..620
                     /locus_tag="U1099_000648"
     CDS             99..620
                     /locus_tag="U1099_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4866989.1"
                     /translation="MQIDPICLEEVSVLQELAKQTFAETFAHDNDPKELEAFFEESYS
                     LEVLTSELSDPNCQHYFLRIDHQIAGYLKLNQGPAQTEQELENAFEIQRIYLLQAFQG
                     RGLGKVLFEFALEKAEQSSCDWVWLGVWEHNYKAQKFYSKYGFEKFGQHEFAVGDKID
                     VDWLLKRPLHQKG"
     gene            622..3021
                     /locus_tag="U1099_000649"
     CDS             622..3021
                     /locus_tag="U1099_000649"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEM4866990.1"
                     /translation="MLVSYKWLKELVDFDATSHDLSEKMSTTGIEVEGVEQKSAGLSK
                     LVVGQVVSAEPIPDTHLNTCQVNVGDEITQIVCGAPNVKPGIKVIVALPGARIAGNYK
                     IKKGKIRGVESLGMLCSLSEIGVSDSVVPKVYADGIYHLPEDAVVGEPVFSYLDLDDE
                     IIELSITPNRADALSMRGVAHEVAAIYDSKVNFKPVVLEESDKKASDVIEVAIESDKV
                     AAYTARVIENVTIAPSPQWLQNLLMNAGIRPLNNVVDITNYILLYFGQPMHAFDFDKF
                     DGKKIVARQAKEGENLVTLDGEERDLITDDIVISVGDKAVALGGVMGGANTEIDNNSK
                     TVVLEAALFDGKSIRKTSGRLNLRSESSSRFEKGINVATLTEAMDTAAAMIAELAGGQ
                     VLSGIVSAGSVDTSDVPVTATIDYVNRSLGTNLDYAQIADIFRRLGMETTGDASQFTV
                     AVPRRRWDIRIPADLVEEIARIYGYNNLPTTLPKEDGTAGELTLTQNIRRQVRSLAEG
                     AGLTEIISYALTTPEKAVEFAARPTTVTELMWPMTVDRSALRQNMVSGMLDTVAYNVA
                     RKNKNLALYEIGKIFEQSGNPKEDLPQEINKFALVLTGLVAEKDFQTPAVPVDFFHAK
                     GILEAIFGHYKLAVDFVATSEIAALHPGRTAEIHLNGAVIGFVGQVHPQTAKDYGIPE
                     TYVAEINLDAIEEALQPAQPFTEISKFPAVSRDIALLLSSDVKHQDVLDAIAGAGVKR
                     LTKVTLFDVYAGSNIEAGKKSMAYNLTFQNPADSLTDEEVAKYMEKISKALVELGAEI
                     R"
     gene            3076..3342
                     /locus_tag="U1099_000650"
     CDS             3076..3342
                     /locus_tag="U1099_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEM4866991.1"
                     /translation="MNTQDIQEKFFREGKLIVIPKKLKSKQVLFAYLQEELAKKGSAF
                     TEKEVNAFLAEIYDDYAILRRYLVDYGYLSRDQYGLEYRIEEKR"
     gene            complement(3363..3665)
                     /locus_tag="U1099_000651"
     CDS             complement(3363..3665)
                     /locus_tag="U1099_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4866992.1"
                     /translation="MYEFVLEYGSFPVKLIDGFVNNRSEIPDFLKEDEEMIARLNEIN
                     ELFHQLFLTIECKFDYIGKQFPDKIEQLRELYHPLADDLLAKYGNQIELKIEPFIL"
     gene            3858..4601
                     /locus_tag="U1099_000652"
     CDS             3858..4601
                     /locus_tag="U1099_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEM4866993.1"
                     /translation="MKTRIVQKQFKRKETKYIVDKKTFELLEKELRQYMMADEFATST
                     ITNIYFDNEDFDMIQDSLAKKNGREKIRMRVYDAIPSESSQAFLEIKKKENKIGYKYR
                     LTSNPVSVANYIENGVIDSTIKDDKVNSELEMLRERYGTIKPKMYIYYDRVSYKGIED
                     KKVRLTIDKNLLYRDYDVDAMEGKFGKNLLDPTKVIMEVKVPEERPDWLVALLEKYQI
                     EKQSFSKYGNAYKLAHNITGEEVSQHAVV"
     gene            4588..5265
                     /locus_tag="U1099_000653"
     CDS             4588..5265
                     /locus_tag="U1099_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEM4866994.1"
                     /translation="MQLFNSIFSTANSNITLAQMSMVFGVSLSMGVLLAAVYKYKSNY
                     TKEFVITLSLMPALIAVIIALVNGNLGAGVAVAGTFSLIKFRSAAGSSKEMLAVLLAM
                     AIGLATGMGFLGLAVFMTVILSACILIFENISFAQVNQNRRHLLVTVPMNFDYDQFFE
                     NQFGKSCKQSDLLSLKYKKKKEALVLEYQVLLDKKITDKRLVDTVLSAGPLDVVLNKQ
                     MPKKKYL"
     gene            complement(5314..5796)
                     /locus_tag="U1099_000654"
     CDS             complement(5314..5796)
                     /locus_tag="U1099_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEM4866995.1"
                     /translation="MTKKPVQLATICYIDNGKEFLLLHRNKKENDVHQGKWIGVGGKL
                     EPGETPQACAIREIFEETGLTVTKHALKGVITFPDFTPNTDWYTYVFKITGFEGSLID
                     CNEGDLEWVPYDQVLSKPTWEGDRHFQEWLLEDKPFFSACFRYDGGKLLDYSVDFYEE
                     "
     gene            5963..6526
                     /gene="folE"
                     /locus_tag="U1099_000655"
     CDS             5963..6526
                     /gene="folE"
                     /locus_tag="U1099_000655"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEM4866996.1"
                     /translation="MSKQEQIEQTIYQLLELLGEDPNREGLLDTPKRVAKMYLEMFNG
                     LEEDPKDQFTAVFSEGHEEVVLVKDIPFHSMCEHHLVPFYGIAHVAYIPSKGRVTGLS
                     KLARAVEVASRRPQLQERLTHQVAHALQDALEPEGVFVMVEAEHMCMSMRGIRKPGSK
                     TVTTVALGKYKEDAILRRELLSMIHNK"
     gene            6535..7347
                     /gene="folP"
                     /locus_tag="U1099_000656"
     CDS             6535..7347
                     /gene="folP"
                     /locus_tag="U1099_000656"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEM4866997.1"
                     /translation="MSIEQLANQVTIMGILNVTPDSFSDGGHYNEVESALVQVEKLLA
                     EGATVIDVGGESTRPGATFVSEEDEIARVVPIIRAIKENYDCLVSVDTYKTGTARAAL
                     EAGADILNDVWAGLYDGDMLALAAEYKVPIILMHNQKDESYADVVGEVKNFLAERAQA
                     ALDAGVSADQIWLDPGFGFSKNVQHNLDLLQGLEQITALGYPVLFGISRKRVVDYLLG
                     GNSLPTDRDQATAALSAWAVQKGCKMVRVHNVAANRDIVKVWDQLTSGGQHG"
     gene            7340..7702
                     /gene="folB"
                     /locus_tag="U1099_000657"
     CDS             7340..7702
                     /gene="folB"
                     /locus_tag="U1099_000657"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEM4866998.1"
                     /translation="MDKISLNKCRFYGYHGAFKEEQVLGQIFTVDCDLFVDLTAASQT
                     DNLEDTVHYGMVFETIKAVVEGKPYILIEKVAGVICQELFDQFPKVEKIRLAIYKENP
                     PIAGHYDSVGIELERERP"
     gene            7699..8187
                     /gene="folK"
                     /locus_tag="U1099_000658"
     CDS             7699..8187
                     /gene="folK"
                     /locus_tag="U1099_000658"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEM4866999.1"
                     /translation="MKHLAYLSIGGNMGNRQAYLQAALDKLASHPGCQLGLVSNIYET
                     PAWGKTDQADFLNLACQVYTDLSAQDFLSACQNIEQELDRVRIEKWGQRTIDLDIIFW
                     DEEKIQEENLIVPHPYAHERAFVLNPLAEIASDYRHPILKKVVSDLLAELGEQKDIKI
                     FK"
     gene            8238..9548
                     /locus_tag="U1099_000659"
     CDS             8238..9548
                     /locus_tag="U1099_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEM4867000.1"
                     /translation="MTILATLGVLLAIVAIIYWTSKNLHVIIAAPLASLIIILTNQMN
                     VLEVMLGKEQSYMAGLAGFLINNFAIFMLGSILARYMEASGATQTIANSILKVMGKDS
                     PYKGLLAITLIASILTYGGVSIFVVIFTLLPLSRPLFKELNINWALFPIPVFLGAGTY
                     TMTTLPGAPSIQNVIPTKVLGTSLTAAPVISLAASLTLLVFGLLYMAYCLKKSLANGE
                     TYTEEEDETAVVVADKTPNLFLSVLPLFSLIGTIFLMSKTPNVLAIGLLVSILLSALI
                     FYPYLPNQKEILNAATTASIVPAFATSSTVAFGTVLTLSAGFAVIQEWIQQIPGSPLI
                     SLSVSTALVSGIIGSSSGAVGIASSNFLPAYLEMGINPELLHRVVVVASAILTVVPQS
                     GVMITFHNLSKLSMKRGLKYSFILVTVGHLLALFVILLLAPLLY"
     gene            complement(9831..11069)
                     /locus_tag="U1099_000660"
     CDS             complement(9831..11069)
                     /locus_tag="U1099_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM4867001.1"
                     /translation="MNQNVTNLKLAERGALLSIGAYIVLSGIKLVAGQLFHSDALRAD
                     AFNNISDIIGNIAVLIGLKMAQKPADTDHKFGHWKMEDLASLITSFIMFVVGFQVLYD
                     TLQKLISNSSVEVDIMGAIVGIFSALVMLAVYLYNNRLAKKVRSKALEAAAKDNLSDA
                     VTSIGTSIAIFAAAFNLPIVDKIAAIIITFFILKTAYDIFMESFFTLSDGFDENLLKK
                     YEEDILKLPKIVSVKSQRGRTYGANIYLDVVLEMNPDLSVYESHEVTEQVEQLLTLKH
                     GVFDVDIHVEPSEIPHDEMYEHVYDKLFRFETEIQAHANGYEELIDDQYLLIDAKGHY
                     RNKTQILADHPIQTTYLSNYQMTSISHKSKLVTFEIGEYVHTSLWRRHENWTVIFHQI
                     SKKQASQIEATEKISDLHNP"
     gene            complement(11126..11578)
                     /locus_tag="U1099_000661"
     CDS             complement(11126..11578)
                     /locus_tag="U1099_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM4867002.1"
                     /translation="MTPVHFLLQALASYIAIAAFLIVLNVQRKMLVPGGLLGMLVWLI
                     YLLLLEPTNVLIATFFAAIIGSCVSQIMSIWLKTPSVIFSLAILAPLVPGYRAYMTTT
                     YFVSGDHAQALTNITTVLTLALVIPIGMASGTILLRLYKVLRTGKKAA"
     gene            complement(11582..12340)
                     /locus_tag="U1099_000662"
     CDS             complement(11582..12340)
                     /locus_tag="U1099_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM4867003.1"
                     /translation="MENTPRLNLVIDVLMLAGAILAQSGAEIHRVEDTMIRIAHSHGI
                     EQANVLAIPAAIFFSIDHTNISRMKRIVNSNYDMQKVCDVNQISRSLANEEISLEEAW
                     SQLNAIKQQKPPYNNIQITLAAVVAAPFFTIMFGGSLLDAAGAVLATALAFPISLIVE
                     RYIGIDFVTTFVGAFVFSLTSYLLSATTTLPINPNLINAGAVMPFVPGIAFTNAVRDL
                     MTNHINTGMTKFFQTALIILSLGAGTTVAFMLVR"
     gene            12501..12926
                     /locus_tag="U1099_000663"
     CDS             12501..12926
                     /locus_tag="U1099_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM4867004.1"
                     /translation="MQEMEDLLYRLKVADETISNLFEKQLGISLTRYSILQTLLKDAP
                     LHQLALQERLQIDRAAVTRHLKLLEESGYIIRKRNPDNQREVLVWPTEQAREALITNP
                     SAHHQAIKTSMNQILTVEESEQFLATLDKLLIGLQNLPI"
     gene            12946..13305
                     /locus_tag="U1099_000664"
     CDS             12946..13305
                     /locus_tag="U1099_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEM4867005.1"
                     /translation="MSIFTTILASIVAIEHLYIMYLETFATHSDSTSRVFNMEKEELQ
                     RKSVTTLFKNQGIYNGLLAVFLIYGIFTGNLEVTTIFVLFVIGAAGYGAATSSPKIIL
                     TQGGPAILALISILLFK"
     gene            complement(13341..14194)
                     /locus_tag="U1099_000665"
                     /pseudo
     CDS             complement(13341..14194)
                     /locus_tag="U1099_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
     gene            complement(14181..14759)
                     /locus_tag="U1099_000666"
     CDS             complement(14181..14759)
                     /locus_tag="U1099_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEM4867006.1"
                     /translation="MNRKEYLQNLEKYLKNLSKEDFQDTLDYFNEYFDEKDNDEEAII
                     ELGTPDEAAKEIIANLYDKERIEDKPNTSNMVWLTILAILAAPIGVPLAITLVALFIT
                     LLLIIFSLFLLLISLWIVLLSLAIAQLLLAFDLLTLSWSSSLLFTGLALVCLALTLLG
                     SKFTLDLGSKTFLLVLHWVQRKIRKGGYYETA"
     gene            complement(14771..15883)
                     /locus_tag="U1099_000667"
     CDS             complement(14771..15883)
                     /locus_tag="U1099_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEM4867007.1"
                     /translation="MTRADYLNQLEQALFQLHPSARQEALDYFNEYFDEKDNDEEAII
                     ELGTPDEAAKEIIANLPEDALIPEKDQASPHSSKPFDFNFDFDFQFDWENFFNNFKHS
                     AYQARERIKLTSKIEELPEFSNLQISLEDQALSVQSYEGEAVLLHNPVSEDGSYQAFD
                     YQLVQDSLYISSKPTPNHLYFSNNQISSAILKIPKKLLPLTNLNLKTADSSLTIVDVQ
                     VCEQMVVNAKDSSISMTKVSCPSSALQLEDSTLTISDGQMSELKLEASDAVITLSSMS
                     LSDARITLEDCVWKLKDFVLEKSLNCQAEDSVLTYKPSTDISLDIWEEDSSLKLPKDK
                     AFTMMKEGDITHLSYKQENSPAQLVIHCQDCQLSVG"
     gene            complement(15880..16197)
                     /locus_tag="U1099_000668"
     CDS             complement(15880..16197)
                     /locus_tag="U1099_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM4867008.1"
                     /translation="MYYPVSSILIEFLVLAIVEKQDSYGYEISQTIKIVADIKESTLY
                     PILKKLEKAGYVTTYTQEFQGRKRKYYTITPAGREHLPFLQGEWNSYKDHIDGIIEGS
                     LRK"
     gene            16328..17242
                     /locus_tag="U1099_000669"
     CDS             16328..17242
                     /locus_tag="U1099_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEM4867009.1"
                     /translation="MNELELLARALGFIFVIGLGYTLKVKGIVRREDARIFSSLVMNI
                     TLPASLLVASATAQVSIDLYVPFILGILCNLFLDMVGYWEAKKSGQQTSVGLMQLSGY
                     NIGTFTLPFVQAFFPLSSLLSVIVFDAGNAIMVLGGNYSIAAGVDEEQERMTVATFIK
                     NLSRSVPLVVYLVSLSLAVCSIKLPRELISALTIASNANPFLAMLMLGILLDLKLNWQ
                     EISRLVYLLSLRLGANILIAGVIYFLLPIDKTMKIMLLVCLVSPISVMSPIYALKLGS
                     RSAEPANVNSLSILVSLVMMVLLIFLFV"
     gene            complement(17278..17676)
                     /locus_tag="U1099_000670"
     CDS             complement(17278..17676)
                     /locus_tag="U1099_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867010.1"
                     /translation="MTEETLAQGILIGIWGTTLLFSFIWYILVAISNYILFKKAGYAG
                     WKSLIPIYNLYIQQCIAFGYEKRWFILFLLIPLAGPLYGIYLVYNFGRSFGLSAVQAI
                     FYVLLTPILNLYIAFNDGSRYQGPQEFFID"
     gene            complement(17805..19025)
                     /gene="pepT"
                     /locus_tag="U1099_000671"
     CDS             complement(17805..19025)
                     /gene="pepT"
                     /locus_tag="U1099_000671"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEM4867011.1"
                     /translation="MKYPTLLDRFLVYVKENTRSDENSTTTPSTQNQVEFAQNILLPE
                     MKRIGLQNVHYLPNGFAVGTLPANDPSLTRKIGFIAHMDTADFNAEGVNPQIIENYDG
                     NPIALGTSGYELHPKDFPQLANYHGQTLITTDGTTLLGSDDKSGIAEIMTAIEFLVQN
                     PDIKHCEIRVGFGPDEEIGVGADKFDVEDFYVDFAYTMDGGPLGELQYETFSAAGAKI
                     DFLGRNVHPGSAKDQMINAFQMAIDFHNALPEADRPEKTEGYEGFFHLMNMEGSVDTA
                     STTYIIRDFEEEDFLARKQLMLDIAEKMNAKFDTPRVIVNLHDQYYNMKKIIEKDMTP
                     INIAKDVMENLGIKPLIEPVRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLETM
                     EKAVDVILGIVAYK"
     gene            complement(19035..19850)
                     /locus_tag="U1099_000672"
     CDS             complement(19035..19850)
                     /locus_tag="U1099_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM4867012.1"
                     /translation="MIKLIALDMDGTLLNEKKELMQPQIDAIHQAVEAGVKIVLCTGR
                     PLAGVKPFVEQLGFDTEEEFIIVNNGCSTHSTKDWSLIDWEELSISDIDYLSTFIEND
                     DVQISLFDEEDYFVLAEKANARVNLDAGLVGMTPQPIVLKEAQSGQHRFFEAMFVGEK
                     EHIDAFENQHNPVLSQHYSTVRSQDYLLEILPNGASKASGLKKLADRLGILPEEIMAM
                     GDANNDLEMIEFAGLGIAMGNANEQVKAIAQAITDTNENNGVAKAIEKHILNK"
BASE COUNT         5611 a         3802 c         4517 g         6006 t
ORIGIN      
        1 gttttcaacc ttgaaccgtc gtaacaccgc tggagtaggt gaacggaaat cgctattgag
       61 cgactttcct agcccagtta gaaatgagag gcaatgagat gcagattgat cctatatgtt
      121 tggaagaggt ttctgttttg caggagctgg ctaagcagac ttttgcagag accttcgctc
      181 acgataatga cccgaaggaa ctggaggcat tctttgagga gtcttattcc ttggaggtct
      241 tgactagtga gttatcggat cccaactgcc agcactattt tctaagaata gatcatcaga
      301 tagctggtta tcttaagctt aaccagggtc ctgcccagac ggagcaggag ttggaaaatg
      361 cctttgaaat ccagcgtatc tatctcttgc aggcttttca aggtcgcggt cttgggaagg
      421 tcttgtttga atttgctttg gaaaaagcag agcagtctag ttgtgactgg gtctggctgg
      481 gagtttggga acataattac aaggctcaga aattctattc caaatacgga tttgaaaaat
      541 ttggccagca tgaatttgcg gtcggtgata agattgatgt ggattggcta ctgaaacggc
      601 ctttgcacca gaaaggataa tatgttagta agttacaaat ggttaaaaga actggttgac
      661 tttgatgcga caagtcatga cttgtctgaa aaaatgtcaa caacaggaat tgaagtagaa
      721 ggggttgagc aaaagagtgc tggcctgtct aagttggttg tcggtcaggt tgtgtctgct
      781 gagccaattc cagataccca cctcaataca tgccaggtca atgtgggtga cgaaatcacg
      841 caaatcgttt gcggtgctcc gaatgtcaag cctgggatca aggtgatcgt cgctctgcca
      901 ggagctcgta tcgctggcaa ctacaagatc aagaaaggca aaatccgcgg tgttgagtcc
      961 ttggggatgc tctgctcatt gagcgaaatc ggcgtgtccg actcagttgt gccaaaggtc
     1021 tatgcggatg gaatctacca cctgccagaa gatgcagtcg tgggtgagcc agtcttttca
     1081 tacctggacc tcgacgacga gattatcgag ctgtccatca caccaaaccg tgccgatgct
     1141 ctctctatgc gtggcgtggc tcacgaagtg gcggctatct atgacagcaa ggtcaacttc
     1201 aagccagtgg tcttggaaga aagcgacaag aaggccagcg atgtgattga agtggctatt
     1261 gagtcagaca aggtggctgc ctacacggct cgtgtcattg aaaatgtgac catcgcaccg
     1321 agcccgcagt ggttgcaaaa cctgctcatg aacgcaggta ttcgcccgct caacaatgtg
     1381 gtggacatca ccaactacat cttgctctac ttcggtcagc caatgcacgc ctttgacttt
     1441 gacaagtttg atggcaagaa aatcgtggct cgtcaggcaa aagagggtga gaacttggta
     1501 actcttgacg gcgaagaacg tgacttgatt accgatgaca tcgtcatttc cgttggggac
     1561 aaggcggttg ccctcggtgg agtcatgggt ggtgctaata cagaaattga caacaactct
     1621 aagacggttg tgctggaagc tgccctcttt gatggcaagt ctatccgcaa gacctcaggt
     1681 cgcctcaacc ttcgctctga gtcttcttct cgatttgaaa agggcatcaa cgtagcgacc
     1741 ttgacggagg ctatggatac agcggctgct atgattgcgg aactggctgg cggtcaggtc
     1801 ttgtctggca tcgtttctgc gggcagcgtg gatacttcgg atgtcccagt cacagcgacc
     1861 attgattacg tcaaccgctc gctcgggacc aatctggact atgcccaaat cgcagatatt
     1921 ttccgtcgtt taggtatgga aacaactggc gatgcaagtc agtttacagt agcagtaccg
     1981 cgtcgtcgtt gggacattcg cattccagca gacttggtag aggaaatcgc tcgtatctat
     2041 ggttacaata atctgccgac cactcttccg aaagaggacg gaacagcagg tgagttgacc
     2101 ctgacccaga acatccgtcg tcaagttcgt agcctggcag aaggtgcagg tctgacagaa
     2161 atcatttctt acgctctgac aacgcctgaa aaggctgtgg agtttgcggc tcgtccaacc
     2221 acagtcaccg aactcatgtg gcctatgact gttgatcgct ctgccctccg tcagaacatg
     2281 gtgtctggta tgttagacac agtggcctac aatgtggctc gtaagaacaa gaacttggct
     2341 ctttacgaaa tcggtaaaat ctttgaacag tctggcaatc caaaagagga cctgccacaa
     2401 gaaatcaata agtttgccct tgtcttgact ggtttggtag ctgagaaaga cttccagaca
     2461 ccagctgtac cagttgattt cttccatgcc aaagggattt tggaagcaat ctttggtcat
     2521 tataagttgg ctgttgactt tgtggcgact agtgagattg ctgccttgca ccctggtcgt
     2581 acagctgaaa tccatttgaa tggggctgtg atcggctttg tcggtcaggt acatcctcaa
     2641 acggccaagg actacggtat tcctgagacc tatgtggctg aaatcaatct ggatgcgatt
     2701 gaagaagctc tccagcctgc tcagccattt accgaaatct ctaaattccc agcggttagc
     2761 cgtgatattg ccctgctctt gagcagtgat gttaaacatc aggatgtcct ggatgccatt
     2821 gctggtgctg gcgtgaaacg cttgactaaa gtgaccctct ttgatgtcta tgcgggtagc
     2881 aatattgagg ctggtaagaa gtctatggct tataacctga ccttccaaaa tcctgcggat
     2941 agcctgacgg atgaggaagt ggctaagtat atggagaaaa ttagtaaggc tttggttgag
     3001 cttggagctg aaatccgtta acatgtaaat cttgtgaaat cctagtcagc gggctaggat
     3061 tttttggagg gaagtatgaa cactcaagat attcaagaaa aattttttcg agaaggcaag
     3121 cttatcgtta ttcctaaaaa attaaaaagt aagcaggtct tgtttgccta tttacaagaa
     3181 gagttggcta agaaaggctc ggcattcaca gaaaaagaag ttaatgcctt tctagctgag
     3241 atttatgatg actatgctat attgagacgc tacttggtgg attatggtta cctatcgcgt
     3301 gaccagtatg gtttggaata cagaatagaa gaaaagagat gattgcggtc atctcttttg
     3361 tcttacaaaa taaagggttc aatcttcaac tctatctggt tgccatactt ggctagtaaa
     3421 tcgtctgcca ggggatgata gagttctcgt agttgttcga tcttgtcagg gaattgtttc
     3481 ccgatatagt caaacttgca ttcgatggtt aagaaaagtt ggtggaagag ttcgttgatc
     3541 tcgttcaagc gggcaatcat ttcttcgtct tctttgagaa aatcaggaat ttctgagcga
     3601 ttgttgacaa aaccgtcgat gagttttacg gggaaagagc cgtattctag gacaaattcg
     3661 tacatgggtt tctccttgcc ttactattga taggactatt gtagcataga ttagaatgtg
     3721 acaaaagaat ttcaacttta ttgcagttta agttttgttt aattttattt tcagtttcat
     3781 ttaaagctaa gtcggtagac tatatcttgt aaacaagaca gggacctgta aaaatttagg
     3841 atagaagagg cacaataatg aaaacaagaa tcgtacaaaa gcagtttaag cgtaaagaga
     3901 ctaaatatat cgttgataaa aaaacttttg aacttttaga aaaggaactt cgtcagtata
     3961 tgatggcaga tgagtttgcg acatcaacga ttaccaatat ctactttgat aatgaagatt
     4021 ttgatatgat tcaagattca cttgccaaaa agaatggacg tgagaaaatt cgtatgcgtg
     4081 tctatgatgc gataccatct gaatcgagtc aagcctttct tgaaattaag aaaaaagaaa
     4141 ataaaattgg ctataaatac cgtttaactt caaaccctgt ttctgtagca aattatatcg
     4201 aaaatggagt gattgattcc acaatcaagg atgataaggt aaactcagaa ctcgaaatgt
     4261 tgagagaacg ctacgggact attaagccga agatgtatat ctactacgat cgcgtatcct
     4321 ataaaggaat cgaagacaaa aaagttcgtc tcacaatcga taagaactta ctttatcgag
     4381 actacgatgt ggatgcaatg gaaggtaaat ttggtaagaa cctattagac ccaaccaaag
     4441 taatcatgga agtaaaagtt ccagaagaac gaccagactg gttggtagcc ctgctcgaaa
     4501 aataccaaat cgaaaagcaa tcattttcaa aatatggcaa tgcctataag cttgcccaca
     4561 acatcactgg agaggaagta agtcaacatg cagttgttta atagtatctt ttcaactgcc
     4621 aatagcaata ttaccttagc gcagatgtcc atggtttttg gagttagtct atccatgggg
     4681 gtactcttag cggctgttta taaatataaa tctaattata caaaggaatt tgtcatcaca
     4741 ctaagtttaa tgccggcatt gattgctgtt attattgcat tagttaatgg taacttagga
     4801 gcgggtgttg cagtagcagg gactttcagt ctaattaaat tccgctcagc agcaggttct
     4861 tcaaaggaaa tgcttgcagt cctcttagcg atggcaatcg gtctagcgac agggatgggc
     4921 ttcctaggtt tggctgtctt catgacggtc atcctatctg cttgtatctt gatttttgaa
     4981 aatatcagct ttgcccaagt caatcaaaat cgtcgtcatc tcttggtgac agtgccaatg
     5041 aattttgact acgatcaatt ttttgaaaat caatttggta aatcttgcaa gcagtctgat
     5101 ttattatccc ttaaatacaa aaagaaaaaa gaagcacttg ttttagaata tcaagtcctt
     5161 cttgataaga aaattacaga taaacgtttg gtagatacag tattgtcagc cggtcccttg
     5221 gatgtcgtat taaacaagca aatgccgaag aagaagtatt tgtaatatca aagaagccct
     5281 ggatttccaa ggcttttttc gttttttatt agcttattct tcataaaaat caacactgta
     5341 atcaagcagt ttgcccccat catagcggaa gcaagcggaa aaaaatggtt tgtcctctaa
     5401 tagccattct tgaaaatgcc ggtcgccctc ccaagttggt ttggacaaga cttggtcata
     5461 gggaacccac tctaagtcac cttcattgca gtcaataaga cttccttcaa aaccagttat
     5521 cttaaagaca taggtgtacc agtctgtgtt tggagtaaag tctgggaagg ttatcactcc
     5581 ttttagagca tgctttgtta cagtaagccc agtttcctca aagatttctc gaatggcaca
     5641 agcctgtggc gtttcgccgg gttctagttt gccaccaact ccaatccatt ttccttgatg
     5701 gacatcgttt tctttcttgt ttcgatgaag gagaagaaat tccttaccat tatcaatgta
     5761 gcaaatcgta gcgagttgga cgggtttctt ggtcatagag gctccttgta gttgatttat
     5821 cctattatac cacaaaggtg ggagtaggga cacctgcttg ttgctctgat aggggacagt
     5881 tttaggatta gcatactgaa agcccttttc tgaaaacagt ttttgaggta taataagaac
     5941 atcaaactat ttaggatgca ctatgtcaaa acaagaacaa atcgaacaaa caatttatca
     6001 attattagaa ttattgggtg aggacccgaa tcgtgaaggt ttattagata cgcctaaacg
     6061 ggtcgctaag atgtatttag aaatgtttaa cggcctagaa gaggatccca aagaccaatt
     6121 tacagctgtc ttttcagaag gtcatgaaga ggttgttttg gtcaaggata ttccttttca
     6181 ttctatgtgt gaacaccatt tggtaccctt ttatggcatt gcccatgtag cttatattcc
     6241 gagtaagggg cgtgtgacag gtcttagcaa attggcgcgt gcagtggagg tagcaagtcg
     6301 tcgccctcaa ttgcaggaac gcttgaccca tcaggttgcc catgcccttc aagatgccct
     6361 tgaaccagaa ggtgtttttg tcatggtcga agcagagcac atgtgcatga gcatgcgtgg
     6421 tattcgcaag ccaggtagta agacagtgac cacagttgct cttggcaaat acaaggaaga
     6481 tgccatcttg cgccgtgaac tcttgtccat gattcacaat aaataggagg ttctatgtca
     6541 atcgaacagt tagcgaatca agtaaccatt atggggattc tcaatgtgac gccagattca
     6601 ttttcagatg gtggtcacta taatgaggtg gagtctgcct tggtacaagt agaaaagttg
     6661 ttggctgagg gtgctaccgt cattgatgtt ggtggtgagt ccactcggcc aggtgcaaca
     6721 tttgtgtctg aagaagacga gattgctcgc gtggttccga ttatccgcgc tatcaaagaa
     6781 aactatgact gtcttgtcag cgtcgatacc tataaaacag gaactgcacg tgcagccttg
     6841 gaagcaggcg cagatatttt aaatgatgtc tgggcaggac tttatgatgg agatatgttg
     6901 gccctggctg cggagtacaa ggtaccaatc attcttatgc acaaccagaa agacgaaagc
     6961 tatgcggatg ttgttggaga ggtcaaaaac tttctagctg agcgtgccca agcagcactt
     7021 gatgctggtg tttccgcaga ccagatttgg ttggatccag gatttggctt ttcaaagaat
     7081 gtccaacata accttgatct cttacaaggt ttggaacaga ttactgctct tggttatccc
     7141 gttctatttg gaatatctcg taagcgcgtg gtggattatc tgcttggggg caatagctta
     7201 ccgactgacc gcgatcaagc aacggcagcc ttgtctgcct gggcagttca aaaaggctgt
     7261 aaaatggtcc gtgttcacaa tgtcgctgcc aaccgtgata tcgttaaggt ttgggaccaa
     7321 ttgacttctg gaggtcaaca tggataagat ttcacttaat aaatgccgtt tctacggcta
     7381 ccatggtgcc ttcaaagaag aacaagttct tggtcaaatt ttcacggttg actgtgattt
     7441 gtttgttgac ctgacagcag cttcacaaac tgacaattta gaagacacag ttcactatgg
     7501 tatggtcttt gaaaccatta aagcagttgt ggaaggcaag ccctacatcc ttatagaaaa
     7561 ggtagcaggt gttatttgtc aggagctttt tgatcaattt ccaaaggtgg agaagattcg
     7621 cttggccatt tacaaggaaa atccgcctat tgctggacac tatgattctg tcggaattga
     7681 attggagcgt gaacgaccat gaagcatcta gcctatctca gtatcggagg gaacatgggc
     7741 aatcggcaag cctatttaca ggcagctcta gacaaattag ccagtcatcc aggttgccag
     7801 cttggcttag tttccaatat ttatgaaact ccagcttggg gcaagaccga ccaggctgat
     7861 ttcctcaatc tagcctgtca agtgtatact gatttgtctg ctcaagattt cctaagcgct
     7921 tgccagaaca tcgaacaaga gttggaccga gtacggattg aaaaatgggg acaacggacc
     7981 attgatttgg atattatttt ttgggatgag gagaagattc aagaagagaa tctgattgtc
     8041 ccccatccct acgcccatga aagagcattt gttctcaacc ctcttgctga gattgcttct
     8101 gattaccgtc acccaatttt gaaaaaagtg gtatcggact tgcttgcaga actaggggaa
     8161 caaaaagata taaaaatttt caaatagttg cctctgatac taggtttatt agaggataga
     8221 atagataaag gagttttatg acaattttag caactttagg agttttattg gctattgtag
     8281 ccattattta ttggacatca aaaaatttac atgttatcat tgctgcacca ctagctagtt
     8341 taatcatcat tctgaccaat cagatgaatg ttttggaagt tatgttgggc aaagagcagt
     8401 cttatatggc gggcttagca ggttttttga ttaataattt tgccatcttt atgttggggt
     8461 ctatcttggc acgttatatg gaggcaagtg gagctacgca gacgattgcc aatagcattt
     8521 tgaaagtaat gggaaaagat agtccctata agggtttgtt agccatcact ttgattgctt
     8581 ccattctgac ctatggtggt gtgagtattt ttgtagttat ttttaccttg cttccccttt
     8641 cacgcccact ctttaaagag ttgaatatca actgggcact ttttccgatt cctgtctttt
     8701 tgggagcagg tacttatacg atgacaactc ttcctggggc accttccatc cagaatgtta
     8761 ttccgacaaa agttttggga actagcttga ccgcggcacc cgttatcagt ttggcagcca
     8821 gcttgacgct attagtattt ggcttgctct acatggccta ctgcctcaag aaaagtctag
     8881 caaatggaga gacgtataca gaagaagagg atgaaactgc agtagtggta gctgataaga
     8941 caccaaatct atttttatca gtcttgcctc tattcagtct tattgggacg attttcctaa
     9001 tgagtaaaac tccgaatgtc ctagcaatcg gattgttggt ctctatcctt ctatcagcct
     9061 tgatttttta tccatatctg ccaaatcaaa aagaaatttt gaatgctgcg acaacagcat
     9121 ccatagtgcc tgcttttgca acttctagta cagtagcatt tgggacggtg ttaaccttgt
     9181 cagcaggttt tgcagtcatt caagaatgga ttcaacaaat accaggatcg ccgctgatta
     9241 gtttatctgt ttcgacagca ttggttagtg gtattatcgg ttcatcttct ggtgcagtag
     9301 gtattgcttc aagtaatttc cttcctgcct atttagaaat ggggattaat ccagagttgc
     9361 tgcatcgtgt agtagttgtt gcatcagcta tcttgacagt tgtgcctcag tctggcgtga
     9421 tgattacctt ccacaatctt tcgaaattaa gtatgaaacg tggactcaag tattcattta
     9481 tcctagtgac agttgggcat ttgttagcct tatttgtgat tttactgttg gcacctttgc
     9541 tatattagga ttgattaaca aacaaataaa agccctgcca atgggcaata tcattcagtt
     9601 aaaatctcta ctcaaatatt tattcgggta gaggcttttt ctttataata taataatcaa
     9661 tgaaatccca aaacagtaag ctctaatctt ctatagttat ttagctttta tcatatacta
     9721 aacaaaaact aaataactac aaattgagta taaaagactg ataatagctc aaagggaagg
     9781 ttaaaaaata ggaattctcc ctataatcca gggaaaattc ctatttttta ttatggatta
     9841 tgcaaatcag agattttttc agtagcttcg atctgagagg cttgtttctt tgaaatttga
     9901 tggaaaataa cagtccaatt ttcatgacgg cgccaaagtg acgtatgaac atattcgcca
     9961 atttcaaacg taactaattt tgatttatgg ctaatagatg tcatttggta atttgataaa
    10021 tacgtcgttt ggatggggtg gtcggcaagt atctgagttt tatttcggta gtggcctttg
    10081 gcatcaatta agagatactg gtcgtcaatc aattcttcat acccattagc atgtgcttgt
    10141 atctctgttt caaaacggaa gagtttgtcg tacacatgct catacatctc atcgtgtgga
    10201 atttcagatg gttctacgtg aatatccaca tcaaaaactc catgtttgag tgttaggagt
    10261 tgctcaacct gctcagtcac ttcatgactt tcataaacgg ataagtctgg attcatttct
    10321 aaaacaacat caaggtagat attggctcca taagtccgac cacgttgtga tttgacagaa
    10381 acgatttttg ggagtttgag aatatcttcc tcgtactttt tcaataagtt ttcatcaaat
    10441 ccatcagata gggtaaagaa gctttccata aaaatatcgt aggctgtttt taggataaag
    10501 aaggtaatga tgatggctgc aattttatcg acaataggga ggttgaaagc cgcagcaaag
    10561 atcgcgatag aagttcctat ggaagtgact gcatcggata gattatcctt ggcagctgct
    10621 tctaaggcct tggaacgtac tttttttgcc aagcggttat tatataaata aacagctaac
    10681 ataactagtg cagaaaaaat tccgacaata gcccccataa tgtctacttc gacagaagaa
    10741 ttgctgatta gcttttggag ggtatcatag agtacttgga aaccaacgac aaacatgatg
    10801 aaggatgtaa tcaagcttgc caagtcttcc attttccagt gtccaaattt atgatcggta
    10861 tcagctggtt tctgtgccat ttttagtcca ataagtacag cgatattacc gataatatca
    10921 gaaatattat tgaaggcatc tgctcgtaag gcatctgaat gaaagagctg tcctgcaacg
    10981 agtttgatac ctgataagac aatataggca ccgatagata gcaaggcacc acgttcagca
    11041 agttttagat tggttacgtt ttggttcata gcatctcctt tgataagttt tcattttaac
    11101 aaaaaggccc cttattttca aggggttagg ctgctttttt gccggttcga agaaccttat
    11161 aaaggcgtaa taatattgtt ccgctggcca taccgatggg aatgactaag gctaaagtta
    11221 agacggtagt gatattggtt agggcttgag cgtggtctcc agaaacgaaa taagtcgtgg
    11281 tcatgtaggc tcgatagcct ggaacaagtg gtgcaagtat agccaatgaa aagatgacgg
    11341 agggtgtttt taaccaaata ctcatgattt gactgacaca ggatccgata attgccgcga
    11401 aaaaggtagc aatcaagaca tttgttggtt ccaatagtaa aagataaata agccaaacaa
    11461 gcatgcctag gagtccacca gggaccagca tttttctttg aacattgaga acaattaaga
    11521 aggctgcgat agcgatataa gaggcgagtg cctgtaataa gaagtgaact ggtgtcatag
    11581 attacctcac aagcataaag gccacagttg tacctgcacc gagcgataag ataataagcg
    11641 cagtttggaa aaatttggtc atccctgtat tgatatgatt ggtcatgagg tctcttacag
    11701 catttgtaaa agcaatccct ggtacaaaag gcatgactgc ccccgcatta atcaggttag
    11761 gattaatggg gagagtagtg gttgcgctta ataaataaga ggtgagtgag aagacaaagg
    11821 cgccaacgaa ggtggtgaca aaatcaattc caatatagcg ctcgacaatt agtgagatag
    11881 ggaaagctaa agctgttgcg aggacagcac ctgctgcatc taaaagagaa cctccgaaca
    11941 tgatggtaaa aaatggagcg gctactacgg cagctaaggt tatttggata ttgttataag
    12001 gtggcttttg ttgtttgatt gcatttaatt ggctccaagc ttcttctagg ctgatttctt
    12061 cattggctag ggaacgggag atttggttga cgtcacaaac tttctgcatg tcgtaatttg
    12121 aattgacaat gcgtttcata cgcgaaatat tcgtatggtc gatggagaaa aagatagcag
    12181 cagggatggc aaggacatta gcctgttcaa ttccgtgtga atgtgcaata cgaatcatgg
    12241 tatcttcaac tcggtgaatt tctgcaccgc tttgtgccaa aatggcacca gcgagcatga
    12301 gcacgtctat gaccaagttt agtctaggag tgttttccat aagtaagtcc catccctctg
    12361 ctagttattt tcttaattat aacaaaatat aggtgtaggc aacatatttt tttatagaga
    12421 ggcaaaatag ttgaaaattg ttcggattat agtataattg acttatcaat gattgattaa
    12481 tcaatcaaaa gaggtagata atgcaagaaa tggaagattt gttatacaga ttgaaagtgg
    12541 cagatgagac gatttccaat ctttttgaaa agcagttggg aatcagtttg acacgctatt
    12601 caatattgca aacattacta aaagatgcac ccctgcatca gttggctttg caggagcggc
    12661 ttcagataga ccgcgcagcg gttacgcgtc atctcaaatt gttggaagag agcggctata
    12721 tcattcgcaa gcgaaatcct gacaatcaga gagaagtact tgtctggcca actgagcagg
    12781 ctagagaagc gctaatcacg aatccttctg cccaccacca agctatcaaa acttcgatga
    12841 atcagattct aactgttgag gagagcgagc aattcctagc cactcttgat aaactgttga
    12901 ttggcttgca aaatctacct atataatttt ttgagaggag acattatgtc tatttttacc
    12961 actattctag ctagtatcgt agctattgag cacctatata tcatgtattt ggaaaccttt
    13021 gcgacccatt cagactctac tagtcgcgta ttcaatatgg agaaggaaga attacaacgt
    13081 aagtctgtga caaccctatt taaaaaccag ggaatctata atggtttgtt ggccgtcttc
    13141 cttatctatg gaattttcac aggcaacctt gaagtgacaa ctatttttgt cttgtttgtt
    13201 attggagcag caggctacgg agcagcgact tctagtccaa aaatcatctt aacccaaggt
    13261 ggaccagcta tcttggcctt gatttctatt ttgttgttta aataagttaa aaagccttcc
    13321 gtgaggaggg ctttttgcaa ttactgaata ttcagttctc catctagtgt agagatatag
    13381 aggtaattag tggcttgggg attggaatag gttggaaggt caattcccag attattttga
    13441 ctggcatcta tatggatgtc agtttttgtt ttgggatgaa gagaaatgtc gattgaacca
    13501 tcgtaattac tgatgccatg ctttccctct agttttaggt tttctgcttt aaaatcacca
    13561 tcctcaacag ttagaatact tctggtagta tcggtgatgt ccgctggact tgttaatgtg
    13621 gactgattga ttgctacttc tccattgatg tgtaatagcc taataaggct gatgtgactg
    13681 ttttcaatca acatgtcacc attttcaaca aagagttgga aatcttgaat agtcacttta
    13741 tccagataga catctccttg aatgctccct gagagtgttt cgattgtgct attttaggga
    13801 cctgtaagac aggttggtag taggcaacat tctggtcatt gacaagattt aagataggtt
    13861 cgagtaaacc atcataagta acgatgcctt gataggcact agaaatggtt aattttccat
    13921 cttggacttg gtgactgagg aggtcaatgt ctttatgctg atttcgatag tagtggaggt
    13981 ggaattggtt gtcaggcgag ggcataatgg acagtttttc attgacctct atagaacgaa
    14041 tatccgtaaa gcttgccgtt tggggctgaa aatagttttc tgcaacagtt ttcatatttg
    14101 tccaaccgcc agccaggtag ccaatgaggc ttagaatggc tccaagacct agcaggctca
    14161 gactgatttt cgtaaatggt ttaagctgtt tcataatagc ctcctttgcg gatctttctt
    14221 tgtacccaat gtaaaacaag caagaaggtt ttgcttccaa ggtctagagt gaatttacta
    14281 cccaatagag tgagggccaa gcagacgagg gccaggcctg tgaagaggag ggaggaggac
    14341 catgagagtg tgaggaggtc gaaggcgagg agaagttggg cgattgcgag gctgaggagg
    14401 acaatccaga gtgagataag gagcagaaag aggctaaaga tgattaggag aagggtgatg
    14461 aagagggcga ccaaggtgat tgctaggggg acgccgattg gggcggcaag gatagcgagg
    14521 atggtcaacc agaccatatt gcttgtattt ggcttatctt caatcctctc tttatcatag
    14581 agattggcaa tgatttcctt tgctgcttca tcaggtgttc ctagctctat aatagcttcc
    14641 tcgtcgttat ccttttcgtc aaaatattca ttgaaatagt ccaaggtatc ttgaaaatct
    14701 tcttttgata ggtttttgag atatttctcg agattctgta aatattcctt gcgattcatg
    14761 gaaacctcct ttatcccaca ctaagctggc aatcttgaca atgaataact agctgagcag
    14821 ggctattttc ttgcttgtag ctgagatgag tgatgtcacc ctctttcatc attgtaaacg
    14881 ccttatcctt tggaagttta aggctagaat cttcctccca gatgtccaaa ctgatgtccg
    14941 tacttggttt ataggttaag acagaatctt cagcctggca gttaaggctc ttttcaagga
    15001 caaaatcctt caacttccag acacagtctt ctaatgttat ccttgcatct gaaaggctca
    15061 tgcttgagag agtgataaca gcatcgctgg cttccaattt taactcgctc atctgtccat
    15121 ctgagatagt tagtgtagag tcttctaact gtagagcaga gcttgggcag ctcactttcg
    15181 tcatgctgat actgctatct tttgcattaa cgaccatctg ttcgcatact tgtacgtcca
    15241 ctattgtcag actactatct gccgttttca gattgagatt ggtaaggggc agtagttttt
    15301 tagggatttt gagaatagca gagctgattt gattgtttga gaagtagagg tgattagggg
    15361 taggcttgct agatatgtac aaactatctt ggactagttg atagtcaaaa gcctggtagc
    15421 taccatcttc tgaaacagga ttgtgcaata agacagcttc tccttcataa ctttgaacac
    15481 taagggcctg atcttcgagc gaaatctgta gatttgaaaa ctcaggtaat tcttcgattt
    15541 tcgacgtcaa tttaattctt tccctagctt gataggctga atgtttgaaa ttgttgaaaa
    15601 aattctccca gtcaaattga aaatcaaaat caaagttgaa atcaaatggt ttagatgaat
    15661 gtggacttgc ttggtctttc tcggggatta gggcatcttc aggtagattg gcaatgattt
    15721 cttttgctgc ttcatcaggt gttcctagct ctataatagc ttcctcgtcg ttatcctttt
    15781 cgtcaaaata ctcattaaag tagtctaaag cctcctgtct ggcacttgga tgaagttgaa
    15841 ataatgcttg ttccagttgg ttcaaataat ctgcgcgtgt cattttcgta agctcccttc
    15901 tataattccg tcaatatggt ctttgtagct attccattca ccctgcagga aggggaggtg
    15961 ttcacgtcca gcaggagtga tggtatagta tttccgcttt ctcccttgaa attcttgggt
    16021 ataggtggtg acatagccag ccttttccaa ttttttcaaa atagggtaga gtgtggattc
    16081 ctttatatca gcaactatct tgatggtttg gctgatttca tagccgtagg aatcctgctt
    16141 ttccacgatg gctaatacca gaaattctat caagatagag gatacggggt agtacataaa
    16201 ctcacctctt tatctttatg taattatttt atatataaat tatataccta ttgtttagca
    16261 aaatcaagcg attatataaa atttttatat atcatcaaaa ttcttttcta tatgctatac
    16321 tagtttcatg aatgaacttg aattattagc ccgtgctctc ggttttatct ttgttatcgg
    16381 tttaggttat acattaaaag tcaaagggat tgttcgaagg gaagatgcaa gaatcttttc
    16441 tagcttagtg atgaatatta cgctaccagc ttctctcctt gtagcttcag cgacagccca
    16501 agtatctata gatttatacg ttcccttcat attagggatt ctttgtaatc tttttttgga
    16561 tatggtggga tactgggaag caaagaagtc tggtcaacag acttccgtcg ggcttatgca
    16621 gttatcgggc tataatatcg gaacctttac actcccattt gtgcaagctt tttttccgct
    16681 atctagtcta ctaagcgtca tcgtatttga tgcggggaat gcaatcatgg ttctaggtgg
    16741 gaattacagt atcgcagctg gtgtggatga agagcaggaa agaatgaccg ttgcaacttt
    16801 tataaagaat ctttcacgct cagttccact agtagtctat ctagttagcc tatcgttagc
    16861 agtctgctct atcaaactac cacgcgaact tatctctgcg ctgacgatag ccagtaacgc
    16921 caatcctttt ttagccatgc tcatgttagg aatcttgctt gatttaaaat tgaattggca
    16981 agaaattagt cgattggtct atcttctctc tttgaggctg ggtgcgaata ttctaatagc
    17041 aggagtcatt tattttcttc tacctattga taaaacgatg aaaatcatgt tgctggtctg
    17101 tctagtctct ccgatttcag tcatgtcccc aatttatgca ttaaagttag ggagtcgctc
    17161 agctgaacct gccaatgtta actccttatc tattttggtt agcctagtca tgatggttct
    17221 tcttattttt ctattcgttt aaaaaagcct ccgcgtgtgc gggggcttga ttgtatatta
    17281 atcaataaag aattcttgtg gaccttggta acgacttcca tcattaaagg cgatgtagag
    17341 gttcaagata ggtgtaagaa gaacatagaa gatagcttgt acagctgata aaccgaagct
    17401 acgaccaaaa ttgtaaacga gatagatgcc atataatggt cctgctagcg gaatcagtaa
    17461 gaatagaatg aaccatcttt tttcatagcc aaaggcgatg cattgttgga tatagagatt
    17521 gtagattgga atcaatgatt tccaaccagc gtagcctgct tttttgaaga ggatgtagtt
    17581 ggaaatagca acaaggatat accaaataaa tgaaaatagt agtgttgtac cccaaatgcc
    17641 aatgagaatt ccctgtgcta atgtttcttc tgtcataata aaaactccat ttctttttta
    17701 tagttctatt atatcagtat tagttcaacg acacaatata aaattgcatt tttttaaaat
    17761 tttttcagaa aaaaacgact ttctattgaa agccgtttga aattttattt gtaagcgacg
    17821 attccaagga tgacatcaac agctttttcc atggtttcca agctgacgaa ctcgaagcga
    17881 ccgtgcatgt tttcgccacc tgcgaagata tttggtgttg gaatgcccat gaaggaaatc
    17941 ttagagccgt ctgttcctcc gcgaacaggc tcaatcagtg gcttgatgcc tagattttcc
    18001 atgacgtctt tggcaatgtt gattggagtc atatcttttt cgatgatttt tttcatgttg
    18061 tagtattggt cgtgcaggtt gacaatgacg cggggtgtgt caaacttggc attcattttc
    18121 tctgcaatat ccagcatgag ctgtttgcgg gcaaggaaat cttcttcctc gaagtcacgg
    18181 atgatgtagg tggtagaagc agtatcaaca ctaccttcca tattcatcaa atggaagaaa
    18241 ccttcatagc cttctgtttt ctcagggcgg tctgcttctg gaagggcatt gtggaaatca
    18301 atagccattt ggaaggcatt gatcatctgg tccttagcag atcctgggtg gacattgcgt
    18361 cctaggaaat caatcttggc accagctgct gagaaggttt cgtactggag ttcgcccagc
    18421 ggtccaccgt ccatggtgta ggcaaagtcc acatagaagt cttccacatc aaacttgtct
    18481 gctccgacac cgatttcttc gtcaggacca aagccgacac ggatttcaca gtgtttgatg
    18541 tcaggatttt ggactaggaa ttcaatcgca gtcatgattt ctgcaattcc tgacttgtca
    18601 tcagagccta gcaaggtagt accgtcagtg gtaatcaagg tttgaccgtg gtagttggca
    18661 agttgtggga agtcttttgg atgcaattca tagccagaag ttcccaaggc aattgggtta
    18721 ccgtcgtagt tttcgatgat ctgcggattg acaccttcag cgttgaaatc agccgtatcc
    18781 atgtgggcga tgaagccaat cttacgtgtc aaacttgggt cgttggcagg cagtgttccc
    18841 acagcaaagc cgtttggtag gtagtggaca ttctgtaagc cgatgcgttt catttcaggc
    18901 agaaggatat tctgtgcaaa ttcgacttgg ttttgggtag aaggtgtggt ggtcgaattt
    18961 tcatctgagc gagtattttc tttgacatag actaaaaaac ggtctaagag agttgggtat
    19021 ttcatagtat ctccttattt gttcaaaatg tgtttttcaa tggctttggc aacgccgttg
    19081 ttttcattgg tgtctgtgat ggcctgggcg atagccttga cctgctcgtt ggcgttgccc
    19141 atggcaatac caagtccagc aaattcaatc atttccaggt cgttgttggc atctcccata
    19201 gccatgattt cttctgggag gataccgagg cggtctgcca gttttttcaa accagaggcc
    19261 ttgcttgcac cgtttggtaa aatttccaag agatagtcct gtgaacgaac ggtggaatag
    19321 tgttggctga gtacaggatt gtgttgattt tcaaaggcat caatatgctc tttttcaccg
    19381 acgaacatgg cttcaaagaa gcgatgctga cctgactggg cttcttttaa aacaattggt
    19441 tgaggagtca ttcctactaa acctgcgtct aaattaacgc gtgcattggc tttttctgca
    19501 agaacgaagt agtcttcctc atcaaaaaga gagatttgca catcatcatt ttcaataaaa
    19561 gtggaaagat aatcaatatc agagatagat agttcttccc agtcaatcag gctccaatcc
    19621 tttgtcgaat gcgttgaaca accgttattg acaatgataa attcttcctc agtgtcaaag
    19681 ccgagctgct ctacgaaagg tttaacacca gccaagggcc gaccagtaca aagaacaatc
    19741 ttgacgcccg cttcaacagc ctgatgaatg gcatcaatct gtggctgcat cagctctttt
    19801 ttctcgttta aaagggttcc gtccatatca agagcgatta gtttaatcat ataaacctcc
    19861 ttgttttcaa tacatctagt atagcacaaa atgagaaagg aatgattgaa atactaaact
    19921 ctgttctata tgttaa
//
LOCUS       DASFSW010000024        19637 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.024, whole genome shotgun sequence.
ACCESSION   DASFSW010000024 DASFSW010000000
VERSION     DASFSW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19637
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.024"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            87..1367
                     /locus_tag="U1099_000673"
     CDS             87..1367
                     /locus_tag="U1099_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEM4867013.1"
                     /translation="MTREFSFETLQLHAGQEVDSASKSRAVPIYQTTSYVFEDAQEAE
                     DLFALRKSGNIYTRITNPTVSTFENRVAALEGGIGALATSSGMAAITYTILALAHAGD
                     HIVAATTLYGGTFNLFKETLPRYGITTSFVDIDDFEAVEAAIKDNTKLVFIETLGNPV
                     INIPDLEKLAEIAHNHHIPLVSDNTFATPYLINVFSHGVDISVHSATKFIGGHGTTIG
                     GVIVDSGKFDWAASGKFPQFVEEDPSYHNISYTRDIGAAAFIVAVRVQLLRDTGAALS
                     PFNAFLLLQGLETLSLRVERHVSNAEKIVDFLLSHPQVESVNYPSLPDSPYKALADKY
                     LPKGVGSIFSFQVKGGAEEARKVIDSLEIFSNLANVADAKSLVVHPATTTHAQLAPED
                     LLAAGVTPNQIRLSVGLENINDLIEDLQLALDKL"
     gene            complement(1445..2977)
                     /locus_tag="U1099_000674"
     CDS             complement(1445..2977)
                     /locus_tag="U1099_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM4867014.1"
                     /translation="MLLATQHLTLDHQKDLRNLVHDLNFIVNPGDKIAIIGEEGNGKS
                     SLLLTLMDSTLVADYLLISGSIHRYFHSPVYIPQSLLLEMAELTLNDYFFSDLDSDID
                     YGLLYLYADQLQFDSERFSGQQLIESLSGGEKLKIQLIKSLATPSDIIFLDEPSNDLD
                     LDTLLWLENYIITSPKTILFVSHDEDFLSRTATKIIHLESVKKKTLAQTKVEELDYQD
                     YALRRQQAYQKQAQKARNDQKEFDKAMNKHLRQKSQVRNTLRNTHDATMGRLIAKKMK
                     NVLSREKRYERMKEKLTEVPFHEDAISLFFSDISPLPARKQVLHLENFQLNVEERQLV
                     SNIHFNINGQEKIGIIGQNGIGKSSFLKLIYQNLRQRADINLGYMPQRYTEVLDESKT
                     PLDFLLENGNREQERLILTHLASLQFTRQEVHHKICELSGGQKAKLLLLKMVLEQVNF
                     LLLDEPSRNFSPTSQPYIRQLFADYPGGLVCVSHDRRFLKEVCQRIYRLTENGLEELE
                     QI"
     gene            3277..3768
                     /locus_tag="U1099_000675"
     CDS             3277..3768
                     /locus_tag="U1099_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867015.1"
                     /translation="MAITGIEQADILEIDKQVRLRRYDGQHDFAFEWHQDLELVWLID
                     GDQERYSLDLLNRMYDYLSKNGECYFIEIFEDNQFIPIGDVTLFADDFAIAIGDRRYW
                     KKGIGTKVLHRMIERARDVGLEEILVEEIYDWNLGSRKLFEKCGFEALEKTKKGWSYK
                     MIL"
     gene            complement(3824..4564)
                     /locus_tag="U1099_000676"
     CDS             complement(3824..4564)
                     /locus_tag="U1099_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM4867016.1"
                     /translation="MAQLKINVHDLHKSYGKNEVLKGITAKFYEGDVVCIIGPSGSGK
                     STFLRTMNLLETITSGQVTVDGYDLTDPKTDVDAFRSNVGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMDKSEAEKVGMELLEKVGLADKRDAMPDSLSGGQKQRVAIARALAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARKVANRVIFTDGGQF
                     LEDGTPEQIFDNPQHPRLKDFLDKVLNV"
     gene            complement(4564..6762)
                     /locus_tag="U1099_000677"
     CDS             complement(4564..6762)
                     /locus_tag="U1099_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEM4867017.1"
                     /translation="MKKKLLMLLASILPVFFIFTGVKADDTIDIVFDNAYAPFEFKDS
                     DQIYKGLDVDIINEVAKRSGWTMNQSFPGFDAAVNAVQAGSADALMAGTTITEARKKV
                     FTFSDPYFDTKIVIATTKANTISSYKDLKGKTVGVKNGTAAQNFLEENKDKYGYNVKT
                     FDTGDLMYNSLSAGAVDAVMDDEAVIQYAIQQGQDLSIDIEGEAIGSFGFSVKKGSQY
                     EYLVEDFNKALAEMKKDGTYETIMNKWLGASTTSAESTDYSSRLSLTGNASAKATPVK
                     SSYTIVADSSFAPFEYQDESGNYVGIDMELIKAIAEQQGFTITIQNPGFDAALNAVQA
                     GQADAVIAGMSITDARKEIFDFSNAYYTSNILLAVKNGSDIASYEDLKGKTVGAKNGT
                     ASYSFLEENKAKYGYTLKAFDEASSMYDSLNSGSIDALMDDEAVLLYAIQQGRNFATP
                     IPGEKSGEYGFAVKKGANPELIEMFNNGLAALVESGKYDEILNKYFNSTEEASTTTST
                     VDETTIVGLLKNNYGQLLSGLGITIGLALLSFAIAIVIGIIFGMFAVSPVKALRVTSS
                     VFVDVVRGIPLMIVAAFIFWGIPNLIESITGQQSPINDFVAGTIALSLNSGAYIAEIV
                     RGGIQAVPAGQMEASRSLGISYGTTMRKIILPQAGKIMLPNFINQFVITLKDTTIISA
                     IGLVELFQAGKIIIARNYQSFRMYAILAIIYLVVITLLTRLARKLEKGGK"
     gene            6963..7622
                     /locus_tag="U1099_000678"
     CDS             6963..7622
                     /locus_tag="U1099_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867018.1"
                     /translation="MIVRYSRWKSLLALGLSLFFGLPLSYVTVDLFISLWGEDVATVI
                     LGGILFLLIFGIPTLASHLVIIVSIQRLFFGLKAFELTEKHFVLYRGIFRRKAVSIPI
                     NEIEFICLEEQTLGAGLSRLFGNVSEKTVVSVGKTIAFATGAKTSARASLSDTYTHLL
                     ILFHDSERAVFYAKQYGIKIKNLLKYTAETSILDIEPGPIEHFSEEKLQDLLEERKLN
                     K"
     gene            7619..9604
                     /gene="uvrB"
                     /locus_tag="U1099_000679"
     CDS             7619..9604
                     /gene="uvrB"
                     /locus_tag="U1099_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEM4867019.1"
                     /translation="MINRNTENQFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVIARVNKPTLVIAHNKTLAGQLYSEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYS
                     DSVVSLRPGQEISRDQLLNSLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREIESLTGKVLGDVDHLAIFPATHFVTNDDHMETAIAKIQAELEEQL
                     KIFEAEGKLLEAQRLKQRTDYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PEDYLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPTMGQMDDLLGEINARVEKG
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADKVTESMR
                     KAMEETARRRQIQMAYNEEHGIIPQTIKKEIRDLISVTKAVTQDKEEVVDFNALNKDE
                     RKAMIKKLEGQMQEAAEVLDFELAAQIRDMVIELKNM"
     gene            9607..11430
                     /locus_tag="U1099_000680"
     CDS             9607..11430
                     /locus_tag="U1099_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HEM4867020.1"
                     /translation="MEVVMKYFDTSKTTNQVVTCIRQLENTELEKELLQFIEECEKAD
                     SLSKEYIERFFKIVDAFLEVFPGSSLDSQHLKKLLTLLHEKIKTEEVWAEIFYRDTSN
                     ISAYSRLFRELIVRVSTYDQIRDFVDRGSIFGTLLESRESVSHTVTRLNLLILIYFVF
                     TFLFISDTDKEKFKLQIDSYIQELLQCDKYLDQMALFYLLSSNKHIQILKREDLRIIN
                     QYVEINIQDNEKFIQQYLDYLKELGADAIAESQKVLQDLNKYTDSDIMKSQIIELLDL
                     TILPVSEPHSSEEIVEKIKGAIDEIRSALIIDAKKLKENEFYGHYTTIDTLVNFLFKP
                     ETEENKNCPTYLRLSNANQLNDPMEGKALLAILGLDAHMEQYYKPTNVFLSSLTVVKD
                     SLPMWKEYAEESRGAFLEYDQDYLETIVKHDVIEFAKVHYITTDEKQNITGDKLVDQK
                     LIYIQDTIKYLEDEGATDEIREIIDYIAKISYLFKVSDYQYESEYRILVNLDDTHFTN
                     KFTDKLNSGIKDEATRRKLDIGIGLLENKNVNYLNSRKYIHIEKTEQGKCNLYAYINV
                     VPLKYSKIMLGPKVENVDYIAPYIHYIQPDIIVETSSIPYR"
     gene            11467..11571
                     /locus_tag="U1099_000681"
                     /pseudo
     CDS             11467..11571
                     /locus_tag="U1099_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
     gene            11758..17792
                     /locus_tag="U1099_000682"
                     /pseudo
     CDS             11758..17792
                     /locus_tag="U1099_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            17866..18369
                     /locus_tag="U1099_000683"
     CDS             17866..18369
                     /locus_tag="U1099_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867021.1"
                     /translation="MMIKPLETKSEILGKAYVHWKSWQEAYADLLPQEFLKNTYTLER
                     CQDWAVRYPQNILIAVMDDKVVGFACYGSSSQEDLQGAGELYALYVLADYYDQKIGYQ
                     LMQAALEKLQSYETVTLWVLEGNARAIAFYEKVGFRFDGVKKTVNLGAERTEYRMIFK
                     QKERENG"
     gene            18362..18517
                     /locus_tag="U1099_000684"
     CDS             18362..18517
                     /locus_tag="U1099_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HEM4867022.1"
                     /translation="MARKEMVTLTNMCLIEDKEGKVVVQIRDPKRYRWSGVAFPGGDC
                     VIIMTGA"
     gene            18654..18827
                     /locus_tag="U1099_000685"
     CDS             18654..18827
                     /locus_tag="U1099_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867023.1"
                     /translation="MQKNEQSSRQIVMCHLMAIMGIDIEKAKRIVAEMEEKELIQFDE
                     LGNVVLLVLEGQS"
     gene            18824..>19637
                     /locus_tag="U1099_000686"
     CDS             18824..>19637
                     /locus_tag="U1099_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM4867024.1"
                     /translation="MKRITADHYQTSERYYKLPKLLFESNRYKGMKLEVKVAYAVLKD
                     RLELSLSKGWIDEDGAIYLVYSNSKLMALLGCSKSKLLTIKKTLREYGLIDEVQQSSS
                     EKGRLANKIYLGELEHEPTPVSNSDVGSVQKTLGGCQNQPGPVLNSATSETEVSETDA
                     SETKESESVIEDEEEKESLTSKKSDNDNFQRKVDRVTRYDRDYIWGLVHDQLRQVGLS
                     RAASDYAMLYFDHRYQYALENMRFADRTETIAEYVFNGVLSEWTKVQRLKELK"
BASE COUNT         6036 a         3635 c         4330 g         5636 t
ORIGIN      
        1 taactataca tatctctaat gtttaagtat tagtcaagct ggtaaaaatg aggtagaata
       61 cagaaaaaga ccaaaggagt acctttatga ctagagaatt ttcatttgaa acccttcaac
      121 tccatgctgg acaagaagtt gattctgctt caaaatcacg tgcagtaccc atctatcaaa
      181 ccacttccta tgtttttgaa gatgctcaag aagcagaaga tttatttgcc ttgcgtaaat
      241 cagggaatat ctatactcgt atcactaatc cgacggtctc tacttttgaa aaccgtgtag
      301 cagctttaga aggtggtatt ggtgccttgg ccacttcttc aggaatggca gccatcacat
      361 acactattct agcattagcg catgctggtg atcacattgt ggcggcaacg acactctatg
      421 gtgggacttt taacctattc aaagaaaccc ttcctcgcta tggtattaca acaagctttg
      481 tagatattga tgactttgaa gcggtagaag ctgcgattaa agataataca aaactggtat
      541 ttattgaaac cttgggaaat cctgttatca atattcctga tcttgaaaaa ttggcagaaa
      601 ttgcacacaa ccaccacatc ccgcttgttt cagataatac cttcgcaact ccatatttga
      661 ttaatgtatt ttctcatggt gttgatatct cagttcattc tgcaaccaag tttatcggcg
      721 ggcatggtac gaccattggc ggtgtgattg tcgatagcgg caagtttgat tgggcggcat
      781 ctggtaagtt tccgcaattt gttgaagaag atccaagcta tcataacatt agttatactc
      841 gtgatattgg tgctgcagca tttattgtag cagttcgtgt acaattgctt cgcgatacag
      901 gtgctgccct ttctccattc aacgccttct tgcttttgca agggttagaa accttgtctc
      961 tccgtgtaga gcgtcatgta tctaatgctg aaaaaattgt tgacttttta cttagtcatc
     1021 ctcaggtaga gtcggtcaat tatccatcac ttccagatag tccttataag gctttggcgg
     1081 ataagtacct gcctaaggga gttggctcta ttttctcttt ccaagtaaaa ggcggagcag
     1141 aagaagcccg taaggttatt gatagtttgg aaatcttttc taatctagca aacgttgccg
     1201 atgccaaatc actagtagtt catccagcta caaccacgca tgctcagtta gctccggaag
     1261 atttacttgc tgctggggtc acaccaaatc aaatccgcct atctgttggt cttgaaaata
     1321 tcaatgattt gattgaagat ttgcaacttg ctcttgataa attataggtt gtgaatcgaa
     1381 tagcatgact tcattcttta tgaataaata aaaggaaatt tggcgtgtgt gccattttcc
     1441 ttttttatat ctgttctagt tcttccagtc cattttcagt tagtcgatag attctttgac
     1501 aaacttcttt caaaaatcgt ctgtcgtgtg atacgcatac caaaccacct ggataatcgg
     1561 caaatagttg acgaatatat ggctgagaag taggcgagaa gtttcgactg ggttcatcta
     1621 ataagagaaa attgacttgt tcaagaacca tttttaacaa gagtaacttg gctttttgtc
     1681 cccccgaaag ttcacagatc ttgtgatgaa cttcttgtcg tgtaaactgc agactggcta
     1741 gatgcgttaa gataagtcgt tcctgctcac gattgccatt ttctaataaa aaatcaagtg
     1801 gagttttaga ctcatcaagg acttctgtat agcgctgggg catgtatccc aagtttatat
     1861 ctgctcgttg cctgaggttc tgatagatga gctttagaaa actggattta ccaattccat
     1921 tttgtccaat aatgccaatt ttctcttgtc catttatgtt gaaatggata ttgctaacta
     1981 attgtcgttc ttcaacgttc aattggaagt tttctaagtg aagtacttgc tttctagctg
     2041 gtagtggtga aatgtctgag aagaagaggg aaatggcatc ttcatgaaaa ggaacttctg
     2101 tgagtttttc cttcattctt tcatagcgtt tttcacgtga taagacattc ttcattttct
     2161 tggcaataag gcgtcccata gtagcatcgt gtgtattgcg caaggtattg cgcacctgtg
     2221 atttttgtcg taggtgctta ttcatggctt tgtcaaattc tttttggtca ttccgagctt
     2281 tttgagcttg tttctgatag gcttgttggc gtctcaatgc gtaatcctgg taatctagtt
     2341 cttcaacctt ggtctgtgca agtgttttct tcttaacaga ttccaggtga atgatttttg
     2401 tagctgttct gctaagaaag tcttcatcgt gagaaacaaa tagaattgtt tttgggctcg
     2461 tgatgatata gttttcaagc caaagtaagg tatctaaatc caagtcattg gacggctcat
     2521 ccagaaaaat aatatcagat ggagttgcta gagatttgat taactgaatt tttagttttt
     2581 caccacctga caggctttca atcaattgtt gaccggaaaa tcgctcactg tcaaactgga
     2641 gctggtcagc atagaggtag agtaggccgt aatcaatgtc gctgtccaag tcagagaaga
     2701 agtagtcgtt caaggttagc tctgccattt ccagtagaag actttgggga atatagactg
     2761 gtgagtgaaa atatcggtga atgctgcctg atatgagtag gtagtcggcg acaagagttg
     2821 aatccatcaa ggttagtaat agactagatt tgccatttcc ttcttccccg atgatggcta
     2881 ttttatcgcc agggttgaca atgaagttta ggtcatgaac cagatttctc aaatcttttt
     2941 gatggtcgag ggttaggtgt tgagtggcta gtagcataag gcctccttta caagaaaaaa
     3001 cacactacct atttagtagt gtgtcgaaat ctgctcaaaa agaccctatt caaggaatag
     3061 agggaacaga aaaatggacg caagaaaact actaaataag tatagaactt aaattgagtt
     3121 tacttgttat tgtccatttc tccttacctc gcatacttta tttattttaa tatagcatat
     3181 cgatagaatc ttgtcaatca gcgtgctata atattcatag atttcgatag agatatatgt
     3241 taaaatgaac ttattgtaaa tgagagagga tccgaaatgg caatcactgg aattgagcaa
     3301 gcagatattt tagaaataga caaacaagtc cgtttacgtc ggtatgatgg tcagcatgat
     3361 tttgcttttg aatggcacca agatctagaa ttggtctggc tgattgatgg tgatcaagaa
     3421 cgttatagtc ttgacttact caatcgtatg tatgattatt tgtctaaaaa tggcgaatgt
     3481 tactttattg aaatttttga agacaatcag tttatcccaa ttggtgatgt gacgctgttt
     3541 gcagatgatt ttgccattgc tattggagat aggcggtatt ggaaaaaggg aatcgggact
     3601 aaggtgttac atcgaatgat cgagcgtgca agggatgtgg gtcttgagga aatacttgtc
     3661 gaagaaatat acgattggaa tcttggttca cgaaagctat ttgagaaatg tggctttgaa
     3721 gctcttgaaa agaccaagaa aggttggtcc tataagatga tattgtaaat taattttgag
     3781 ataccaaaaa aaccttgacg caagtcaagg cttttcgttt agcttagacg ttcaagacct
     3841 tatcaaggaa gtctttgaga cgtgggtgtt gtgggttgtc aaagatttgc tcaggagtgc
     3901 catcttcaag gaattgaccg ccatcagtaa agataacgcg gttagcaacc ttgcgggcga
     3961 agcccatttc gtgtgtgacg atcaacatgg tcataccttg ttctgccaag tccttcataa
     4021 cgttgagtac atcgccaacc atttcagggt caagggcaga agttggttcg tcaaagagca
     4081 tgatgtctgg gttcatagcg agggcacgag cgatggcaac acgttgcttc tgaccacctg
     4141 aaaggctgtc tggcatagcg tcgcgtttgt ctgccaatcc aactttttcc aataattcca
     4201 tgccgacttt ttcagcttca gacttgtcca tcaatttgtg ttcgattggt gcaaatgtga
     4261 tattgtcaag tacagacata tgagggaaga gattgaagtg ttggaatacc attccgacat
     4321 ttgaacggaa ggcatcaaca tctgtcttag gatcggttaa gtcatatcca tcgactgtta
     4381 cttgaccact tgtaatggtt tcaagtaagt tcattgtacg aaggaaggtc gatttacctg
     4441 aaccagaagg accgatgata caaacaacat cgccttcata gaacttagca gtaatacctt
     4501 tgaggacctc gtttttacca taagacttgt gtaagtcatg gacgttgatt tttaattgag
     4561 ccattatttg cctccttttt cgagtttgcg tgctaagcga gtcaagagtg tgataactac
     4621 caagtaaatg atggcaagaa ttgcatacat acggaaggat tggtagttac gtgcaataat
     4681 gattttacct gcttggaaga gttcgaccaa accaatcgca gaaatgatag tagtatcttt
     4741 cagagtaata acaaactggt tgataaagtt aggcaacatg attttaccag cctgtggcaa
     4801 gataatctta cgcatggttg ttccatagga aatacccaaa ctacgagatg cttccatttg
     4861 acccgcagga actgcctgaa taccaccacg aacaatctca gcaatatagg caccagagtt
     4921 gagggatagg gcaatcgtac cagcgacaaa gtcattgatt ggagattgtt gaccagtaat
     4981 cgactcgatt aagtttggaa tgccccagaa gatgaatgca gcgacaatca tgagcggaat
     5041 accacgaaca acatctacaa aaacagatga agtaacacgg agtgctttaa ctgggctgac
     5101 agcaaacata ccgaagataa taccgatgac aattgcaata gcaaatgata gaagggcaag
     5161 tccgattgtg ataccaagac ctgagagcaa ttgaccgtag ttgtttttca aaagaccaac
     5221 aattgttgtc tcatcaacag ttgaagtcgt tgtgcttgct tcttcagttg agttgaaata
     5281 cttattaagg atttcatcat acttgcctga ttcaactagg gcagctaagc cattgttaaa
     5341 catttcaatt aattctgggt tggcaccttt tttaacggca aatccgtact cacctgattt
     5401 ttcgccagga attggtgttg caaagttgcg accttgttgg atggcatata gaagaaccgc
     5461 ttcgtcatcc ataagagcat caatagaacc agagtttaaa ctgtcataca tacttgaagc
     5521 ttcgtcgaag gcttttaggg tgtaaccata ttttgccttg ttttcttcta agaaactata
     5581 tgaagcagta ccatttttag ctccaacagt tttccctttt aagtcttcgt aagaagcaat
     5641 gtcactacca tttttaacag ctaataggat gtttgaagta tagtaggcgt ttgagaagtc
     5701 aaatatttcc ttacgagcat ctgtgataga cataccggca ataactgcat cagcttgtcc
     5761 agcttgaaca gcatttagtg cagcgtcgaa accagggttt tgaatggtaa tagtaaatcc
     5821 ttgttgttca gcgattgcct tgattaattc catgtcaatc ccgacatagt tacctgattc
     5881 atcttggtat tcaaatggag caaaagaaga atctgccaca attgtgtagc ttgatttgac
     5941 aggtgttgct ttagctgatg cgtttcctgt taagcttaaa cgagatgagt aatctgtact
     6001 ttcggcagaa gtggtagaag ctcctaacca tttgttcatg attgtttcgt aggtgccatc
     6061 tttcttcatc tcagctaaag ccttgttaaa gtcttcaaca agatattcat attggctacc
     6121 tttttttaca gagaaaccaa atgaaccgat tgcttcgcct tcgatgtcaa tgcttaggtc
     6181 ttgaccttgt tggatagcgt attgaataac cgcttcgtcg tccatgacag catcaacagc
     6241 accagcagaa aggctattat acatcaaatc gccagtatca aaagttttta cgttgtatcc
     6301 gtacttgtct ttgttttctt ctaggaagtt ttgggctgca gtaccgtttt tgacaccgac
     6361 agtcttgcct ttaaggtcct tatatgaaga gatggtattt gcttttgtgg tagcgataac
     6421 gattttggtg tcgaagtaag ggtctgagaa agtaaatact ttcttacgcg cttcggtgat
     6481 agttgtacct gccattaggg cgtccgcaga tccagcctgt acagcgttta cagctgcatc
     6541 aaatcctggg aagctttgat tcatggtcca acctgagcgc ttggctactt cgttgatgat
     6601 gtcaacatct aatcctttat aaatttgatc tgagtcttta aactcgaatg gggcgtaagc
     6661 attgtcaaat acgatatcaa tagtatcgtc agctttaacg ccagtaaaaa taaagaatac
     6721 aggcaaaatg cttgccaata acatgagtaa ttttttcttc atttccatac tccttctatt
     6781 ataaaataac tgtcctagta tatcatattc tgaaaattaa tgcaaaatcg aggtttttat
     6841 aatgttagaa tttctgacat gcaaagtcat taaaatgtga gaaaatttaa tgttttaggg
     6901 caattgattt tgagtaagtt caagcggact tgtggtaaaa tagaatagtg aaaggaatga
     6961 gtatgattgt tcgttatagc agatggaaat ctttgttggc cttgggtctg agtttgtttt
     7021 ttggcttgcc attgtcttat gtaacggttg atttatttat cagtttatgg ggagaagatg
     7081 tagcgacagt gatcctcggt gggattttat ttttactgat ctttggcatt ccaaccttgg
     7141 cttcacatct ggttattatc gtttctatac agcgcctctt ttttggatta aaagcatttg
     7201 aactgacaga gaagcacttt gttctctatc ggggaatatt tcgaagaaaa gctgtgtcca
     7261 ttcctataaa cgaaattgag tttatctgcc tagaagaaca gacactagga gcaggcttga
     7321 gccgtctttt tggaaatgtt tctgaaaaaa cagtagtatc tgtagggaag acaatcgcat
     7381 ttgcgactgg ggctaaaacg agtgcaaggg ccagtttgtc tgacacctac acgcacttat
     7441 tgatattgtt tcatgacagt gagcgagctg tcttttatgc taaacaatat ggaattaaga
     7501 taaagaattt attgaaatac acggcggaaa cttcgattct tgatattgaa cccggcccaa
     7561 ttgagcattt ttccgaagag aagttgcaag atttactaga agaaagaaaa ctgaacaaat
     7621 gataaatcga aatactgaaa accaatttaa acttgtttct aaatactcac cgtctggtga
     7681 ccagccccaa gccattgaaa ccttggttga taacatcgag gggggcgaaa aagctcagat
     7741 tctcatgggg gcgactggta cgggtaagac ctacaccatg agtcaggtca ttgcccgtgt
     7801 caataagccc actcttgtca tcgcccacaa caagactcta gctggtcagc tctacagcga
     7861 gttcaaggaa ttcttcccag aaaatgcggt cgaatacttc gtatcttact atgattacta
     7921 ccagccagaa gcctatgtac cgtctagcga tacctatatc gagaaggaca gttcggtcaa
     7981 tgatgagatt gacaaacttc gtcattcagc gacttcagca cttctcgaac gaaatgatgt
     8041 catcgttgtg gcttcggttt cctgtatcta cggtttggga tcgcccaagg aatattcaga
     8101 tagcgtggtc agcctgcgac caggtcagga gatttcccgt gatcagttgc tcaactctct
     8161 ggtagatatt cagtttgagc gcaacgacat cgacttccaa cgggggcgtt tccgtgtacg
     8221 tggagatgtg gtggagattt tcccagcttc ccgtgatgaa cacgcctttc gtgtggagtt
     8281 ttttggtgat gaaatcgacc gcattcgtga gattgaaagc ctgacaggga aggttttggg
     8341 cgatgtggat cacttggcca ttttccctgc cacccatttc gtgaccaacg atgatcacat
     8401 ggaaacggct attgccaaga ttcaggcgga actggaagag cagctcaaga tctttgaggc
     8461 agaaggaaaa ctcttagaag ctcagcgatt gaaacaacgg accgactacg acatcgagat
     8521 gcttcgagaa atgggctaca ccaacggagt tgagaactat tcacgacata tggacgggcg
     8581 aagcgagggc gagcctccct acaccctgct ggactttttc cctgaagatt acctgattat
     8641 gattgacgag agtcatatga ccatggggca gattaaggga atgtacaatg gtgaccgctc
     8701 acgcaaggag atgttggtca actatggttt ccgcctcccg agtgcactgg acaaccgtcc
     8761 gctgcgcagg gaagaatttg agagccatgt ccaccagatt gtctatgtat ctgcgacgcc
     8821 gggtgactat gaaatggagc agaccgatac cgttgtcgag cagattattc ggccgacagg
     8881 gcttttggat ccagaggtgg aagtccgtcc aaccatgggc caaatggacg acctcttggg
     8941 cgaaatcaat gcccgtgttg agaagggtga gcggaccttt atcactaccc tgaccaagaa
     9001 aatggcagag gacttgaccg actacctgaa agaaatgggc gtcaaggtca agtatatgca
     9061 ctcggacatc aagaccttgg agcgtacgga gattatccgt gatcttcgct tgggtgtctt
     9121 tgatgttttg atagggatta acctgcttcg tgaagggatt gacgtgccag aagttagtct
     9181 agtcgctatc ttagacgcag acaaggaggg cttcctccgt aatgaacggg ggctcatcca
     9241 gacaatcggt cgagcggctc gtaactctga aggtcatgtg attatgtatg cggacaaggt
     9301 caccgagtcc atgcggaagg ccatggaaga aacagcccgc cgccgtcaaa tccagatggc
     9361 ttataatgaa gaacatggca tcattccaca aactattaag aaagaaatcc gtgacctgat
     9421 aagcgtgact aaggctgtca ctcaggacaa ggaagaagta gtggacttca atgctcttaa
     9481 taaagatgaa cgcaaggcta tgatcaagaa actggaaggt cagatgcagg aagcagcaga
     9541 agtgcttgac tttgaactgg cagctcaaat tagggatatg gtcattgagt tgaagaatat
     9601 gtagatatgg aggtagttat gaaatatttt gatacgtcga aaacaactaa tcaagtggta
     9661 acctgtatta gacaattgga aaatacagag ctagaaaaag agttgttaca gtttattgaa
     9721 gagtgtgaga aagcagactc tctatccaaa gagtatatag aacgtttttt taaaattgtc
     9781 gatgcttttc ttgaggtgtt tccaggatca tccttggatt cgcagcactt aaagaaactc
     9841 ttgactttgc tccatgaaaa aataaaaaca gaagaggtgt gggcggaaat cttttataga
     9901 gatacctcta acattagtgc ttattctaga ttatttaggg aattaattgt tcgagttagc
     9961 acttatgatc aaattagaga ttttgttgat aggggaagta tttttgggac gctattggaa
    10021 agtagagaaa gtgtgagtca tactgtaacc agactcaatt tactaatact gatatatttc
    10081 gtatttacct ttttgtttat ttctgatacg gataaagaaa agtttaaatt acagatagat
    10141 tcctatattc aggaattact tcaatgtgat aagtatttag accaaatggc cttattttat
    10201 ttgctatcca gcaataaaca tattcaaata ttgaagagag aagacttacg tataattaat
    10261 caatatgtag aaataaatat acaggataat gagaagttta ttcaacagta ccttgactat
    10321 ctaaaggaat tgggggcaga tgcaattgca gaatctcaga aagttctaca agacttgaat
    10381 aagtatacgg attctgacat tatgaaaagt cagataatag agttactaga tttaactatc
    10441 ttgcctgtta gcgaacctca ttcaagcgag gaaatcgttg agaaaataaa gggagctatt
    10501 gatgaaattc gttctgcatt gataattgat gcaaagaaat taaaagagaa tgaattttat
    10561 ggccactata ctacaataga caccttggtt aatttcttat ttaaaccaga gacggaagaa
    10621 aataaaaatt gtccaaccta tttacgtttg tcaaatgcta atcaattgaa tgatccgatg
    10681 gaaggaaaag ccttactcgc catccttggt ttagatgccc atatggaaca gtattataaa
    10741 ccaaccaatg tgtttctttc atccttgaca gttgttaaag atagtttacc tatgtggaaa
    10801 gaatatgctg aggaatctag gggagcattt ctggaatatg atcaagatta cttggaaact
    10861 attgtcaagc atgatgttat tgaatttgct aaagttcatt atattactac agatgaaaag
    10921 caaaatatta ctggtgataa actagttgat caaaaactaa tatatattca agatacaatt
    10981 aagtatttag aagatgaggg agcaaccgat gaaattcgag aaatcattga ttatattgct
    11041 aagatatcct atctttttaa ggtttctgat tatcaatatg aatctgaata tcgtatattg
    11101 gtaaacttag atgatacgca tttcactaac aaattcactg ataaattgaa ctctggaata
    11161 aaagatgaag ctacacgtag gaaactagat attggcatag gattgttaga aaataagaat
    11221 gtgaattatc tgaacagcag aaaatatatt cacatagaaa aaacagaaca agggaaatgt
    11281 aatttatatg cgtacatcaa tgttgtccca ttaaaatatt caaagattat gttggggcca
    11341 aaagttgaaa atgtagacta tatagctcct tatatccatt acattcagcc tgacattatc
    11401 gttgaaactt caagtattcc gtatcgataa cctcttttga aaagctacga agaattgagt
    11461 tagaccataa gtaagaaact ggaaggtcag atgcaggaag cggcagaagt gcttgacttt
    11521 gaactggcag cccagattcg tgatatggtc atcgagttga agaatatgta ataagagttg
    11581 ctaagttcag tgatttaagt agaatgaaat tacaagaggc ggcaggtaga aattgacctg
    11641 ccacctttta taatacttaa aatttttact tggagactta gggacaaaaa atttgtccct
    11701 gtccctataa aaggttataa tggtttctat actatttagg gaataggggg agtttttatg
    11761 aaaaatattt ttggtgaaaa acgacagcgg ttcggttttc ggaaactgtc aattggactt
    11821 gtctcggcag ctgtagcaag tttgtttttt gcgtcatcgg tcgcagcagc accgtcagct
    11881 agtgctcagt ctatcaatta ttcctatgtg acagagcaag aattgacaga tgcagaaaag
    11941 gagttgatta ttcgtgactt gcctggctta gctcaggaaa cggatgcaaa ttattacctg
    12001 atctatcgtc cagctactgg cacaacatct actccatcga caagcacgtc tcaagttttg
    12061 ccaaatactg gctctgtaga aaccggacta ttggtggcgg gtggagtgtc tctcctgtta
    12121 ttggctgtta gatttggtaa aaaaggtaag aaggagttgg cgggtgtcat ccttttgaca
    12181 gctacgggag caagcttctt cggtccaact agctccgcat tgacgagtca aattttggct
    12241 cgatataacc atgcaatcga aatatctgcc ggacaagcgt taccggcccc agctgagatt
    12301 gatggctacg tttatgtagg atatttgaag gatagcaaag ctattgaaca aactacttca
    12361 gaaggaatcc gtacagaaac cattgtcaac aagacagagg tcattccatt tgaaattcaa
    12421 accgtagaaa atcccaatta tctgcgggga cagagcgagt ggtacaggaa ggacaggatg
    12481 gggagcgtac tgtaacgatt aaacaagtcc attcaaaagg tcaaattatt tcggaagaag
    12541 agatttcctc gactgttaca aaaactgctg tttcaaagat tgttgaagtt gatacaaaac
    12601 aagcaaccga tgatattgtt acagaagtac cagatacagc gcctagtcac gagttaccta
    12661 ccttacagat aacggaagaa gtatctacct acacggagtc tattcctttt gaaacacaag
    12721 aagttgctga tgataccttg ctagaaggta caagacagat tgtgcaagaa ggtaaagctg
    12781 gacaaaagac aattgagaca aagaaaacct acatcgacgg tattttagta aaaacagaaa
    12841 ctgtctctga aacgattaac caacctgctg ctccacagat tatccatgtt ggaacaaagc
    12901 cagtcacaag tgtaccagat tcggcaccta gccatgaagt tccaacgatt tctttaacgg
    12961 aagaaacaca tactcataca gaagctattg cctttgatgt gcaagaaatc tatgatgcaa
    13021 acttggcaga aggcagccgt gaagttgaac agaagggaca agctggtgtt cggacaatcg
    13081 agactaagaa ttattatgcc gatggtgtac taattaaatc cgaacaagtt tcagatgtag
    13141 tgacaaaaga accagtaaca gaagttgtaa gagtcggtac gaagacaaca gatgttattg
    13201 gatctgaaac cattgtaaca acagaagagc ttccgtttga aacgacagtc acagaaacag
    13261 aagaacttta tgttggggaa gaaaaattca ttactgaagg taaggttggt tctaaggaag
    13321 ttaccactac ttatcagaca atcaacggag tccgtcaacc agccccaaca gtgactgaga
    13381 aagtccttga agagcctaca acgaaagagg ttctcaaagg aacaaaaccg atagagggta
    13441 cggaaaccga gactaatcaa gttgaaattg cctttgaaac agagtatgtt gatgacccga
    13501 cattgttaga aagtaagacc aaagttgtaa cggcgggtgt aaatggctct aagacagtta
    13561 caacaactta ccagactatc aagggtgttc gtcaggaaaa ttctactgta acagaagaaa
    13621 ttaccaaaca acctgttaag caagtcattg cgcgtggtac aaaggttgaa aaagtaccac
    13681 aagtgattat cacagatttg gtggaaaatg acgatgccaa gtcagcgaca atcagctaca
    13741 agttgacaga cgaaaccgct aatttccaac gagctgttgc tctcctctat gataatacag
    13801 gtgccttggt tcaagaacag accattacag atccaaatgg ccagttgaca cttgaaaatc
    13861 ttgatttcta cacagattac actgttaaga ctaagatttt ctacacaatg gcagagcagg
    13921 aacaaagttc ggaacaagaa gctattcttg aaagcatacg caaatttgat ttggtctaca
    13981 agaagattga aatcaaggat attgatgcgg tgactgttta ccgtcgtaaa aatggtagct
    14041 acatcggtca agaattcttg gaagaactgc caacatccac cgatgaattg tttatcaagg
    14101 tgacttcaga ccgcttcaag gaagtatacc tacctgtgtc aagcattgaa gaaacaacct
    14161 tgaatggcaa ggcagtcttc aaactagttt catcctttga tgaattggta caggacaagg
    14221 atgcccaata cgttgccaac cgtgaattct acattcctaa gatggcgact gatgcaaata
    14281 cctacacatc cttcaaagct ttgctcgacg ctatgaaggc aaatccaagt ggtaccttta
    14341 aactaggagc gcatttggac gcaagtgaag ttcctgttgg agatgttgca tcgtatgtga
    14401 ccaactttag cggtaccttg gatggcttaa atgacggcta cgccttttct atcagcaact
    14461 tgaaagcacc attgttcttt aaccttggtg gtaaagttca aaacttggac atcaaaaatg
    14521 caagcctgaa tacaagctct aagaacccac ttgcgacaat tgcgattaat gccaatggag
    14581 caactattac aaacgtagca gtagaagcat ctatcaaagg accgcaaaac gtatctggtt
    14641 tagttcattc tgcaacaaat actactatta aaggtgtatc cttcaagggt agtatcgaag
    14701 taaccggaac caatgcaagt ctaactggtg gtatcttggg taacggtacg atggcaagtg
    14761 tcggaaatgc caaggttgat gcgaccataa ccctcccagg tacagagaat caagtagctg
    14821 gtggtatcgt cggtagaact atgctcgttt atgacgtacc aggaagtgtc tataattcgt
    14881 atgcaacagg ttcaatcgtg acaaatggta cagaggcaac cattggtgga attgctggtt
    14941 ccaaccaagt gactggcgat cttgctccac acgtaggtaa tttgacaaat gttgttagca
    15001 ccatgaacgg tcctacaagt ctagttggtt tagttgtcaa tccaactggt aagattaaag
    15061 acggcttcac aacaacttca gatacattgg gcaatgtcaa ggctatcacg aatgaagaag
    15121 cacaagcaaa agttgaagcc atgaagattc aagcgacctc gtcagattcc aaacctctca
    15181 atcttaaccc ttattctgtc aattatttaa cactagataa ggctcaggca gaccatgaaa
    15241 cagcctacta caacatggag aaaatcttac cattctacaa caaggaactc cttgtttatt
    15301 atggaaataa gattgcggtt gatgataagc taaacaaggt tcgtttactg gatgttgttc
    15361 caatgaaaga tactaccttt attgcagatg tccataggga aaaagcaaac atcaataaga
    15421 ttatgcttca ctatgctgat gacacggtag attataaggc agtttcatac cttgaagact
    15481 tcaagaataa tcatgtggtc gagtacacca tttcaggtac ggacttgatt tacacgccag
    15541 aatcattctt gaatgacaga agtgcccttg tcaatgactt ggtaagcagc ctttcttctg
    15601 ttgtcctcga ctcggatgct atgaaggcgg ttatcaatta tccaacaaaa ttaaatgcgg
    15661 atactcagac tggcacagct aaagacttct actttggtga cagctataac caagtaatgg
    15721 ctaacctaga aagcaacgtt cgtaagattt tggttgctag tctaaatggt caaggtcagg
    15781 cttctgaaga ctacatcaaa gagaaaatca ccaataacaa agcagccttt gtgctcggtt
    15841 tgacttactt gaaccgttgg tatgacatca acttcggaga gatgaatacc aaagacttga
    15901 ccatctttga acctgacttc ttcggaaatg atgtagcatc tgcccttgat atgattcttg
    15961 caattggtaa tggtggctat gatgttcttc gtgcccacaa taacgtgacc acttttgcat
    16021 ccatcattgg taaacaaaat aatcagacca agcttttcga tatgctggaa gattatcgtc
    16081 agctcttctt gccagaaatg accaacaatg aatggttcaa gcaaacaacc aaggcttatg
    16141 ttgttgaagg taaatccttg attccagaag tagctgctaa gcaggaaacg acagatacat
    16201 atagcaagta caatgttgga gcttatagca agattgtgaa tgatactgta tccaatccaa
    16261 cttggaagta caaccacatg ctcttgccac tcttgacttt gccacaagaa aatatcttta
    16321 tcatcactaa catgaatacc atcgcgattg gttcatacga acactacgta gatgattatt
    16381 cgacagtgga aaatcgtgat aaggttcgtc agatggttga tttggcggca gaacgtcagc
    16441 gtgataatgc ggatttctgg tacaagattt tggatgaaac caaccgtgac aagctattca
    16501 gatctgttct caataatgaa ggctacatga tgtttggtcc agatggtacc aaggcttatc
    16561 gcaatttaac tgctgatgtt gatgctattc aagacttcta cggtccgatc aacaagtggt
    16621 atcgtgagca tccatctatt aagactgctt atgcggacgg aagtgaaacc tactatatca
    16681 cttacgatat gctgtcagat tatggtacag ccctttatac ccacgaaatg gttcacaacc
    16741 aagatggtga tatttatctg aaaggctatg gtcgccgtat tggtcaaggt atggaagttt
    16801 acgcccaagg tcttctacaa aatgtcttca atgttactga gatgaacctt ggattcaacg
    16861 ctgtctataa tagcgatgat gccaaccgtg tccatgtcgg agacccagtt gcacgtttca
    16921 atagcgaagc tgatttcaat gagtattacc ataaccagtt cgatgtgctg tacttgctgg
    16981 attacctaga aggtacaaac atcttggctc aatcggatgc agcgaagaaa gcatggttgc
    17041 gtaagattga aaactactat gtccaaaata atggtgttga tacccatgct ggaaactctg
    17101 cgcgtgcatt gacagatgcc gaagtggcaa gcttgaagtc cttcaatgat ttgattgatc
    17161 agagtattat cgttcaacgc caatatgtca acaatccaac taacacaagc aagaaatggg
    17221 atagaaatag ctatgtcagc gttccgatgt ttgcggctaa cttctcagcc ctttcaaact
    17281 caaacggttc accaggcgac atcatgttcc gtcgtatggc ctttgaattg attgctgcca
    17341 aaggctacac agatggtttt gtgccgtatg cttccggaca attatccgac ctagccatgg
    17401 aaaaaggttc taagatttac gatacatgga ctaaaaaaga tgttggtttg attacagatg
    17461 accatgttct ggaatatatc taccaaggtc gatatgctac atgggctgac ttcaagaaag
    17521 ccatgtttgc tgaacgcttg gcaaaagtag cagctggcca gctcaaacca ttcaccatgc
    17581 aatacgaatt aggtgttgct gattcaacca aggaagtaac catcacaaac tttgaacagt
    17641 tgcaaacctt gatgaaagaa gctatggagg cagacatcca agcaaatagt ttaaacttga
    17701 acaacagtcg tgttcatgca ctcaaagtga aagtttatca agcgttgatg aactctacaa
    17761 atgacttcag aacatcgatt tttaacccat aatcataaac aagttggtct tgtgccaact
    17821 tgttttttaa tgtatactag aggaaaaaat tcggaggtac attagatgat gattaaacca
    17881 ctggaaacga aaagtgaaat actaggcaag gcctatgttc attggaaatc gtggcaggag
    17941 gcctatgctg acttgttacc tcaggaattt ttaaagaata cttacacctt agaacgctgt
    18001 caagactggg cagttcggta tcctcagaat atcttgatag ctgtgatgga tgataaggtg
    18061 gttggatttg cctgttacgg ctcgtctagt caggaggatt tgcaaggggc aggggagctc
    18121 tacgccctct atgtcttggc ggactattat gatcagaaga taggctatca gctcatgcag
    18181 gcagctttgg aaaaattgca gagctacgaa accgttactc tctgggtatt ggaggggaat
    18241 gcgcgtgcca ttgccttcta tgaaaaagtg ggcttccgat ttgatggtgt caaaaaaacg
    18301 gtcaatttag gtgcagaacg tacagaatat agaatgattt ttaaacagaa agagagagaa
    18361 aatggctaga aaagaaatgg tcactttgac caacatgtgt ctaatcgaag ataaggaagg
    18421 gaaggtcgtt gtacagattc gagatcccaa gcgttatcgt tggtctggtg ttgcctttcc
    18481 aggcggggat tgtgtcataa taatgacagg tgcttaaaac gctgttaaat caaggatttg
    18541 agtgttctac aatctgtcat taaggaggaa ttttttagat tttataaata atatcgcttt
    18601 agcgagaaca ttttaataag tgttctcgct tttttatttt caggaggaaa atcatgcaaa
    18661 agaatgaaca gtcatcacga caaattgtga tgtgtcatct catggctatc atgggaatcg
    18721 atattgagaa agcaaagcgg atagttgctg aaatggagga gaaagaactc attcaatttg
    18781 atgagttggg aaatgttgtt ctgttagtct tggaggggca atcatgaaac gtatcaccgc
    18841 agatcactat caaacgtcag agcgttacta taagctccca aaactcttgt ttgagagtaa
    18901 tcgctataag ggcatgaaac ttgaggtgaa ggttgcttat gctgttctaa aagaccgttt
    18961 ggaattatct ctgagtaaag gttggattga tgaagatggg gctatctatt tggtctattc
    19021 caactcaaaa ctcatggcac tgcttggctg ttccaagtca aaattgctga cgattaagaa
    19081 aaccttacga gagtatggtt tgattgatga agtccaacag tcttccagtg aaaaaggacg
    19141 tttggcgaat aaaatttact taggtgagtt ggaacatgaa cctaccccag tctcaaattc
    19201 agacgtgggt agtgtccaaa aaacactagg ggggtgtcaa aatcagccgg ggccggtctt
    19261 aaattcagcc actagtgaga ctgaagttag tgagactgat gcgagtgaaa ctaaagagag
    19321 tgagtcagtc attgaggacg aggaggagaa agaaagtcta acaagtaaga aaagtgacaa
    19381 tgacaatttt caacgtaaag tagaccgagt cacaagatat gacagagatt atatttgggg
    19441 tttggttcat gaccagttga gacaagttgg tctatcacga gccgctagtg attatgccat
    19501 gctttatttt gatcatcggt atcagtacgc tttagagaat atgaggtttg cggacagaac
    19561 agaaacgata gcggagtatg tctttaatgg cgtcttatcc gaatggacca aggtccaacg
    19621 cttaaaagaa ctaaaag
//
LOCUS       DASFSW010000025        18190 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.025, whole genome shotgun sequence.
ACCESSION   DASFSW010000025 DASFSW010000000
VERSION     DASFSW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18190
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.025"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(263..1435)
                     /locus_tag="U1099_000687"
     CDS             complement(263..1435)
                     /locus_tag="U1099_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEM4867025.1"
                     /translation="MIPFALKYIMVIITTMVISAIATPLVRFMSLRVGAVDNPNARRI
                     NKVPMPSAGGLAIFVAFTISVLWFLPGIVTRVNYHGTYLHYIWPLVLASSIIVLTGFI
                     DDVKELKPAPKMFGILAAATIIWIFTEFRFDSFKIPFGGPFLQFPVWLSFLLTVLWIV
                     SITNAVNLIDGLDGLVSGVSIISLTTMGIVSYFFLHDSNIFLTLTIFVLVAAIVGFLP
                     YNYNPAIIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPMIILGVPITDTVVAIIR
                     RKLSGQKIYEADKMHLHHRLLSLGLTHRGTVLVIYTISFLFSLTSLLLNVSSRIGGIL
                     LLVTMGLGIEILCELIGIFGINRTPLLNLLRFIGNSSYRQEKIAKYKKCCEEKKRK"
     gene            complement(1432..2154)
                     /gene="mecA"
                     /locus_tag="U1099_000688"
     CDS             complement(1432..2154)
                     /gene="mecA"
                     /locus_tag="U1099_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEM4867026.1"
                     /translation="MKVKQISDSTLKITIKLDDLEERGMELSDFLIPQEKTEEFFYTV
                     LDELDLPMTFRESGMLSFRVTPKPDRVDIFVTKSDLDQNLNFDEFTDLSELGDVASMT
                     PDEFFKSLEQTVREKSAPDATAVRHLEEVEQEEEADEEEQERYIYYILEFPTIEDLFT
                     FVGTVDYPVEESELYKMDGHYYLTVLINVENRSKQYPGYILSRMLEFTNDTKLTRPAL
                     QEHGTLMLPLAAIEELRKVPTV"
     gene            complement(2282..3118)
                     /locus_tag="U1099_000689"
     CDS             complement(2282..3118)
                     /locus_tag="U1099_000689"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEM4867027.1"
                     /translation="MLLELLKAIFLGIIEGVTEWLPVSSTGHLILVQEFVKLNQSKNF
                     LEMFNIVIQLGAILAVMTIYFKKLNPFQPGKTKRDIQLTWQLWAKVVIACIPSILIAV
                     PLDNWFEAHFNFMVPIAIALIVYGIAFIWIENRNSGVEPQVTDLAKMSYKTALLIGCF
                     QVLSIVPGTSRSGATILGAIILGTSRSVAADFTFFLGIPTMFGYSGLKAVKYFLDGNS
                     LNMEQVWILLVASMTAYLVSLVVIRFLTDFVKKHDFTVFGYYRIILGAILLVYAFITF
                     LF"
     gene            3245..4810
                     /locus_tag="U1099_000690"
     CDS             3245..4810
                     /locus_tag="U1099_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEM4867028.1"
                     /translation="MKRKLSIFLLTLIAVFSVTTGVRADEYLRVGMEAAYAPFNWTQE
                     DDSNGAVPIEGTNQFANGYDVQVAKKIAASMDKELLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTEERKKEIAFSDSYYTSIPTLVVRSDSQYANATSLADFAGAKITAQQGVYLYD
                     LIDQIEGANKQTAMGDFSQIRQALESGIIDAYVSERPEGRTAEAANKAFKMVELADSF
                     ETNAEDVTIAVGMRKDDERIAQVNKVLATISEEEQIALMDDMIENQPVEEASEGETPS
                     FFAQVWNIVVNNWQQLLRGTGMTLLISILGTIIGTAIGLLIGVFRTAPKAANKALAIG
                     QKVLGWIINIYIEIFRGTPMIVQSMVIYYGTAQAFGLNLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHNQTMRKIVLPQVIRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTVIAIIYFILTFTVTRILRAVEKHFDTDTYT
                     TGANQMQVEVPHD"
     gene            4803..5546
                     /locus_tag="U1099_000691"
     CDS             4803..5546
                     /locus_tag="U1099_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM4867029.1"
                     /translation="MTKNVILEIKNLKKSYGQNQVLKDISISVEKGEVISIIGSSGSG
                     KSTFLRSINLLETPTEGQILYHGQDVLDKGYDLTTYREKLGMVFQSFNLFNNLNVLEN
                     AIVAQTTVLKRDRAEAEKIAKENLNKVGMTEQYWQAKPSQLSGGQKQRVAIARALSVD
                     PEVILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMLIVTHEMEFARDVSDRVIFMDKG
                     VIAEEGNPQQIFENPQEERTREFLQRFLG"
     gene            complement(5670..5879)
                     /locus_tag="U1099_000692"
     CDS             complement(5670..5879)
                     /locus_tag="U1099_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867030.1"
                     /translation="MTSYEKVAAVVGWVREKKITGYRISKETNAREMSVIALAQGRAK
                     IDNISFATALGLIDFYDKNHKKFEN"
     gene            complement(5965..6867)
                     /locus_tag="U1099_000693"
     CDS             complement(5965..6867)
                     /locus_tag="U1099_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM4867031.1"
                     /translation="MVLGPIVFIGSFLFFVLIALILIASGLYVVKQQTVAIIERFGKY
                     QKTSTSGINFKIPFGVDVIAARIQLRMLQSEIVVETKTQDNVFVTMNVATQYRVNENN
                     VTDAYYKLMHPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQKQVAEEMSTYG
                     YVIVKTLITKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRL
                     HGVGIAQQRKAIVDGLADSIRELKESNVSLSEEQIMSILLTNQYLDTLNNFAQGGNQT
                     IFLPGNPEGVEDIRTQILSSLRAK"
     gene            complement(6877..8127)
                     /gene="ilvA"
                     /locus_tag="U1099_000694"
     CDS             complement(6877..8127)
                     /gene="ilvA"
                     /locus_tag="U1099_000694"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEM4867032.1"
                     /translation="MITAKNVEQAYSVLKRVVVRTPLDYSRYLSEKYGATIYLKRENE
                     QRVRSFKIRGAYYAISQLTDDEKSRGVVCASAGNHAQGVAYTCQEMQIPATIFMPITT
                     PQQKIGQVKFFGGDYVEIRLVGDTFDESAKAAQDYTQESGKTFIDPFDDENVQAGQGT
                     VAYEILEEAEEQTISFDQILVPIGGGGLVAGVSAYLKEHAPEIKIVGVEASGARSMKA
                     AFDKGRPVKLDQIDKFADGIAVQKVGKSTYEVARKYVDRLIGVDEGWISGTILDLYSK
                     LGIVAEPAGAATIAALEVVKDKIKGQTICCIISGGNNDINRMPEMEERALIYEGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRANKGTGPVLIGIALGDKTDYTQFI
                     SRLEEFDPQYINLHENDSLYKMLV"
     gene            complement(8411..9433)
                     /gene="ilvC"
                     /locus_tag="U1099_000695"
     CDS             complement(8411..9433)
                     /gene="ilvC"
                     /locus_tag="U1099_000695"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEM4867033.1"
                     /translation="MTVTMQYEKDVTVAALDGKRIAVIGYGSQGHAHAQNLRDTGHDV
                     IIGVRAGKSFDKAKEDGFETFEVAEAAKQADVIMILAPDEIQADLYNEEIAPNLEAGN
                     ALGFAHGFNVHFEFIKVPADVDVFMCAPKGPGHLVRRTFEEGFGVPALYAVYQDATGN
                     AKHIAMDWAKGVGSARVGLLETTFKEETEEDLFGEQAVLCGGLTALMQAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLVYEGGFKKMRQSISNTAEFGDYVSGPRVITDQVKENMK
                     AVLADIQSGKFANDFVNDYKAGRPRMEAYRKEAENLEIEKVGAELRKAMPFVGRNDDD
                     AFKIYN"
     gene            complement(9565..10041)
                     /gene="ilvN"
                     /locus_tag="U1099_000696"
     CDS             complement(9565..10041)
                     /gene="ilvN"
                     /locus_tag="U1099_000696"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEM4867034.1"
                     /translation="MRRMLTAKLRNSSGVLNRFTGVLSRRQINIESISVGPTEVDGIS
                     RVTVIVDVVSHDEVEQIIKQLNRLIDVVRVRDLTDIPHLEREVILVKISAPPTKRAEI
                     LAIIQPFRANVVDVAPHSITIQMTGDGDKIDALLRVIQPYGIKNIARTGATGFSRD"
     gene            complement(10034..11737)
                     /locus_tag="U1099_000697"
     CDS             complement(10034..11737)
                     /locus_tag="U1099_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEM4867035.1"
                     /translation="MQEIQLDQPRSGSYLILDTLHQLGVDLVFGYPGGAVLPLYDAIY
                     QYEGIQHILARHEQGAVHEAEGYAKSSGKVGVAIVTSGPGATNAITGIADAMGDSVPL
                     LVFTGQVATRGIGKDAFQEADVLGMTMPITKYNYQIRDTADIPRVITEALHIATTGRP
                     GPVVIDVPKDIQERVVDFYHDPTLHLPSYQPTIEPNGLQVKKILKQLSQAQKPVILAG
                     GGVNYADANKELVAFAERYRIPVVSTLLGLGAMPIEHELSLAMGGMHGSYAANMAMDQ
                     ADYIINIGARFDDRLTGNPTTYAPNATVAHIDIDPAEIGKVVKTAIPVVGDAKATLKA
                     LLALETVETGYADWTAQVLENKRRAPFWYDEDEKVIKPQVAIELIGQLTQGDAIVVTD
                     VGQHQMWAAQFYPYKHARQLVTSGGMGTMGFGIPAAIGAKLANPDKEVIIFVGDGGFQ
                     MTNQELAILNGYGVPIKVVLINNHSLGMVRQWQESFYEKRRSQSVFDDEPNFQLLAEA
                     YGISHYSFDNPATLSEDMKVILEDKPMLIEVHISNREHVQPMVPAGKSNAEMLGVKFN
                     A"
     gene            11962..13011
                     /locus_tag="U1099_000698"
                     /pseudo
     CDS             11962..13011
                     /locus_tag="U1099_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
     gene            13001..13934
                     /locus_tag="U1099_000699"
                     /pseudo
     CDS             13001..13934
                     /locus_tag="U1099_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
     gene            14487..16202
                     /gene="ilvD"
                     /locus_tag="U1099_000700"
     CDS             14487..16202
                     /gene="ilvD"
                     /locus_tag="U1099_000700"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEM4867036.1"
                     /translation="MTDTNKLKDFRHRSSVYDSMVKSPNRAMLRATGMTDDSFEKPIV
                     GVISTWAENTPCNIHLHDFGKLAKEGVKDAGAWPVQYGTITVADGIAMGTPGMRFSLT
                     SRDIIADSIEAAMGGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAVFAYGGTIAPGNL
                     NGKDIDLVSVFEGIGKWNNGDLTAEEVRQIECNACPGPGGCGGMYTANTMATAIEVMG
                     MSIPGSSSHPAESPEKKADIEEAGRAVVRMLELGIKPSDIMTREAFEDAITVTMALGG
                     STNATLHLLAIAHAANVELTLEDFNDFQERVPHLADLKPSGKYVFQDLYNVGGVPAVM
                     KYLLKNGFLHGDRITCTGKTVAENLENFADLTPGQDVIMPLENPKRADGPLIILKGNL
                     APEGAVAKVSGVKVRNHTGPAKVFDSEEEAIEAVLTDEIVDGDVVVVRYVGPKGGPGM
                     PEMLSLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDLV
                     TVDQDTKEITMHVSDQEIEDRKKTTVIPPLYSRGVLGKYAHTVSSASKGAVTDFWRPE
                     RTGKK"
     gene            16253..16639
                     /locus_tag="U1099_000701"
     CDS             16253..16639
                     /locus_tag="U1099_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867037.1"
                     /translation="MKLNVELSRFRVKEGKTVQVDEWMAFLNEHMEDTLLTLEGEKMY
                     VETIFREVLDGREYLYWYSVQAEGRIEVEDSESYIDKKHLEYWEECIDPSYGMVGLDP
                     QVIMIHKTIYETMEELDRQYDETFKK"
     gene            complement(16666..17007)
                     /locus_tag="U1099_000702"
     CDS             complement(16666..17007)
                     /locus_tag="U1099_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HEM4867038.1"
                     /translation="MREDIQINERALTVSEQLVEVLESIYDPEIELDIYNLGLIYEIH
                     LDEAGFCKVVMTFTDSGCSCADTMPGELVAALKTIDGINDAQVEIVWSPAWKMTRISR
                     LGRITLGISPK"
     gene            complement(17129..17548)
                     /locus_tag="U1099_000703"
     CDS             complement(17129..17548)
                     /locus_tag="U1099_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEM4867039.1"
                     /translation="MELKDIMHILEDMKVGVFATLDEYGNPHARHAHITAANEEGIFF
                     MTSPETHFYDQLMGDQRVAMTAISEEGYLIQVVRVEGTARPVENDYLKTVFADNPYYQ
                     HIYKDESSDTMQVFQIYAGHGFYHSLPQGHKYIFSIG"
BASE COUNT         5437 a         4113 c         3418 g         5222 t
ORIGIN      
        1 aacgttgaga agcggtacgg ggcttgagcc cagcctcact ccactggact gttaaaccca
       61 atcaaccact actgctctga gatgttgacg ctaactttaa gaagcggtgc ggggcttgaa
      121 cccagcctct tttcttgtta atgatgattt tcagagagtg ttaatctata aaatagtctt
      181 ttctgacaat tttaattaac catatccact atattatatc aaaaaaagag cctgacggct
      241 cttatcttgt ttagaaagat tcttattttc tttttttctc ttcacagcac tttttatact
      301 ttgcgatttt ttcttgacgg taactactat taccaataaa ccgaagaaga ttgagcaatg
      361 gagttcgatt aataccaaag atgccaatca attcacagag aatctcaatt cccagtccca
      421 tggtcaccaa taggagaata ccccctattc gactcgaaac attgagcaaa agagaggtca
      481 gggagaataa gaaagagatg gtgtaaatca ccaatactgt gccacgatga gtaagtccca
      541 gagagagcaa gcgatgatgc agatgcatct tatctgcttc atagattttt tgcccagaca
      601 attttcgacg aatgatggct actacagtat ccgtaatagg cactccaaga ataatcattg
      661 gagtaaccac tgcaacagct gtcgaatttt tcagtccttg aagggacaag actccaatca
      721 taaacccgat gaaaagggct cctgtatcgc caagataaat gatagcggga ttatagttat
      781 aaggtaaaaa tccaacaatc gctgcgacca aaacaaagat ggttaaggtc aaaaaaatat
      841 tgctatcatg caaaaagaaa taagacacaa ttcccatcgt tgtcagggaa ataatcgata
      901 ctcccgaaac tagaccatcc agaccatcaa tcaagttgac tgcattcgta atagaaacaa
      961 tccaaaggac cgttaataga aaagagagcc agacaggaaa ttgaaggaaa ggtcctccaa
     1021 aaggaatctt aaagctatca aaacgaaatt ccgtaaaaat ccaaatgatc gtcgctgcag
     1081 ctagaatacc aaacatttta ggagcaggct ttagctcctt gacatcatca atgaatcccg
     1141 taagaacgat aatgctggat gctaggacca ggggccagat ataatgcaag taagtgccat
     1201 ggtaattgac acgggtaaca attcctggta aaaaccatag gacagaaatg gtaaatgcca
     1261 caaagatagc cagacctccc gctgaaggca tgggcacctt atttattcgc cgtgcattgg
     1321 gattatcgac tgctccaacc ctcagggaca taaaacgaac caagggggtt gcaatagctg
     1381 atatgaccat agttgtaata atgaccatta tgtattttag agcaaaagga atcatactgt
     1441 cggaaccttc ctaagctcct cgattgccgc aagcggaagc atcaaagttc cgtgttcttg
     1501 taaagcaggt ctggtcaact tagtatcatt ggtaaattcc aacattcttg agagaatgta
     1561 gcctggatac tgcttggaac gattttctac attgataaga actgtcaagt agtagtgacc
     1621 atccatttta tataattccg attcttctac tggataatca accgtaccaa caaaagtaaa
     1681 cagatcctct atcgttggaa attccaaaat atagtaaata tagcgctcct gctcctcttc
     1741 atctgcttct tcctcttgct ctacctcttc caaatgacga actgctgttg catccggtgc
     1801 acttttctca cgaacggttt gctccaaact cttgaaaaac tcatctggag tcatacttgc
     1861 gacatcacca agttccgaca aatctgtaaa ttcatcaaaa ttcaagttct gatccaaatc
     1921 agatttggta acaaagatgt ccacccgatc tggcttcgga gtcacacgaa aactcaacat
     1981 accgctttct cggaaagtca ttggtaaatc caattcatcc aaaacagtat agaagaactc
     2041 ctctgtcttt tcttgcggaa tgagaaagtc cgaaagttcc attcctctct cttccaaatc
     2101 atctaatttt atagtgattt tcaaggttga atcactaatt tgctttactt tcataactac
     2161 ctcattcatt gattcatcaa tttcttaaaa ttgattgatc attttcaata ctcatacata
     2221 tcttccacca ttatactaaa accaagctcg aaagacaaaa gaaaagcagg tctccctgct
     2281 cttaaaataa aaatgtaata aaggcataaa ccaataaaat cgcccctaaa atgatgcggt
     2341 agtaaccaaa aaccgtgaaa tcatgttttt taacaaagtc tgtcaagaaa cgaataacaa
     2401 ctaaggatac taagtaagcc gtcatactag cgactagcaa gatccatacc tgctccatat
     2461 tcaggctatt gccatctaag aaatatttaa cagccttcaa gccactataa ccaaacatgg
     2521 ttggaatccc aaggaagaag gtaaaatctg ccgccacaga tcggctggtc cccaaaataa
     2581 tcgctcccag aatggtcgca cctgagcgac tagtccccgg aacgatgctt aagacctgaa
     2641 aacacccaat caacaaggca gttttataag acattttcgc taagtccgtc acctgtggct
     2701 ctacaccact gttacggttc tcaatccaaa taaaggcaat accgtagaca atcaaagcaa
     2761 tcgcaatcgg taccataaaa ttgaagtgcg cttcaaacca attatccaac gggactgcaa
     2821 ttaggatgga cggaatacag gcaatgacaa ccttggccca taattgccaa gtcaattgaa
     2881 tatcacgctt ggtttttcca ggctgaaatg gattcaattt tttaaagtaa atagtcataa
     2941 ctgctagaat tgctcctagc tgaatgacaa tattaaacat ttctaggaag ttcttgcttt
     3001 ggtttaattt cacgaactct tgaaccaaaa ttaaatgccc cgttgaagaa actggcaacc
     3061 attccgtcac cccttcaatg attcctaaaa atatagcctt caacaattcc aatagcataa
     3121 aaatctcctt ttcatcaatt ctctacatta tatcataaaa aaactcccta ttcatagtac
     3181 aaggatattg aaatttgtca gaatttttaa taaaatggtg taatagcatt ttaaaggaga
     3241 cattatgaaa cgtaaactca gtatatttct gctgacccta atcgctgttt tttctgtaac
     3301 aacaggggtt cgggcagacg aatacctgcg ggtcggtatg gaagctgcct atgcaccatt
     3361 taactggacc caagaagatg atagcaacgg tgccgttcct attgagggaa ccaatcaatt
     3421 tgccaacgga tatgatgttc aggttgcaaa aaaaatcgct gcatccatgg acaaagaact
     3481 cttggttgtt aaaaccaagt gggaaggctt agtccctgct cttacatctg gtaaaatcga
     3541 tatgattatc gccggtatga gcccgacaga agagcgcaag aaagaaatcg ccttctcaga
     3601 tagctactac acttcaattc ctaccctggt tgttcgttct gatagtcagt acgcaaacgc
     3661 aaccagcttg gcagattttg ctggcgcaaa aatcactgcc cagcaagggg tttacctcta
     3721 tgatttgatt gaccaaattg aaggggctaa caaacagact gccatgggag atttctcaca
     3781 aatccgtcaa gctttagaat ctggcattat agacgcctat gtttctgaac gtccagaagg
     3841 tcgtacagca gaagctgcaa ataaagcttt caaaatggtt gaattggcag acagttttga
     3901 aaccaatgcc gaagacgtga ccattgctgt cggtatgcgc aaagatgacg agcgtatcgc
     3961 tcaggtcaac aaagtcttag caactatttc agaagaagag caaatcgcac ttatggatga
     4021 tatgattgaa aaccagcccg ttgaagaagc cagcgagggc gaaacaccta gtttcttcgc
     4081 ccaagtttgg aatattgttg ttaacaactg gcaacaactt ctgcgcggaa ctgggatgac
     4141 attactcatt tccatcctcg gtacaattat cggtactgct atcggtctcc tcatcggtgt
     4201 cttccgtaca gctccaaaag cagcaaacaa ggcgcttgct attggtcaaa aagtgctcgg
     4261 ttggattatc aatatctaca tcgaaatttt ccgtggtacg ccgatgattg tacaatccat
     4321 ggttatttac tatggtaccg cccaagcatt cgggctcaat cttgaccgca cacttgccgc
     4381 tatcttcatt gtatccatca acacaggtgc ctacatgagt gagattgtcc gcggtggtat
     4441 ctttgccgtc gataaaggcc aatttgaagc tgctactgct ctcggattta ctcataatca
     4501 gaccatgcgt aagattgtcc ttccacaggt tattcgtaac atcttaccag cgactggtaa
     4561 tgaatttgtc atcaatatca aagatacttc tgtattgaac gttatctcag ttgttgaact
     4621 ctatttctca ggaaatactg tcgcaaccca aacctatcaa tatttccaaa cctttacggt
     4681 tattgctatt atctacttca tcctaacctt tactgtgaca cgtatcttac gcgcagttga
     4741 aaagcatttt gatacagata catacacaac aggtgcaaac caaatgcaag tcgaggtgcc
     4801 acatgactaa aaatgtaatt cttgaaatca aaaatctaaa gaaatcatac ggacaaaatc
     4861 aagtcctcaa agacatctct attagcgttg aaaagggcga ggttatctca atcatcggtt
     4921 catctggttc gggaaaatca accttcctcc gatctatcaa ccttttggaa acaccaacag
     4981 aaggacagat tctataccac ggacaagatg ttttggataa aggatatgac ttgacgactt
     5041 accgtgaaaa actaggtatg gtattccaat ccttcaacct tttcaacaat ctaaatgtat
     5101 tggaaaatgc cattgtagca caaactacgg tactgaaacg agatcgagct gaggcagaga
     5161 aaatcgccaa ggaaaacctc aacaaggttg gcatgaccga acagtactgg caagccaaac
     5221 caagtcaact ctctggtgga cagaaacaac gcgtggccat tgcacgtgcc ctatctgttg
     5281 acccagaagt tatcctcttt gatgagccga cttctgctct cgatccagag atggtcgggg
     5341 aagttctcaa aaccatgcaa gatttggcaa aatcaggatt gacgatgctt atcgtaactc
     5401 acgaaatgga atttgcccgt gacgtatctg accgcgttat ctttatggat aagggtgtca
     5461 tcgccgaaga aggcaaccct caacaaatct ttgaaaatcc acaagaagaa cgcacacgtg
     5521 aattccttca gcgttttctt ggttaaactc aatctgttcc atagaagacc attacttacc
     5581 agtttcaaaa ctggtaagtt tttttgacta aaaaaaagag gaatcgcaaa attcctcttt
     5641 ttggacatct ctaaatgaaa ccatttagat tagttttcaa atttcttgtg gtttttgtcg
     5701 taaaaatcaa tcaaacccaa ggcagttgca aatgaaatat tgtcaatctt agcgcgacct
     5761 tgtgcaagag cgataactga catttcacga gcatttgttt ccttactgat acggtaacct
     5821 gtgattttct tttcacgaac ccagccgaca acagcggcta ctttttcgta actagtcatt
     5881 agaaacccct ttctttttta ttcgtgcaat ttagtataat atgttttaac ctattttgtc
     5941 aagtaaaatg ttcgtttttt accatcattt tgccctaaga gatgatagta tttgcgtcct
     6001 tatgtcttcg accccctccg gattccccgg aaggaagata gtttgattac ctccttgtgc
     6061 aaaattattg agagtatcca agtattggtt ggtcagcaag atagacataa tctgctcttc
     6121 agataggcta acatttgatt ctttcagttc acgaatcgaa tctgccagtc cgtctacaat
     6181 cgccttacgt tgttgtgcaa tacctacacc atgcaagcga tccttctcag cctctgcttc
     6241 tgctgctgtg acaattttaa tcttgtcagc ttctgccaat tcttgggccg caacccgttt
     6301 ccgctgagct gcgttaattt cattcataga ttgcttaacc tcggcgtctg gctctacctt
     6361 ggtaattaag gttttgacaa taacataacc gtaggttgac atctcttctg ctacttgttt
     6421 ttgtacctca agtgcaatct catccttttt ctcgaagagc tcatccaggg tcaatttagg
     6481 cacagatgaa cgcagagcat cttcaatata ggacttaatc tgtgcttctg ggtgcatgag
     6541 cttgtaatag gcatccgtca cattattttc atttactcga tattgagttg ccacattcat
     6601 tgtgacaaaa acgttatcct gcgtcttggt ttctaccaca atttcacttt gcaacatccg
     6661 caattgaatg cgagctgcaa tgacatcaac tccaaatggt attttaaagt taatacctga
     6721 agtagatgtt ttttggtatt tcccaaagcg ctcaataata gcgaccgtct gctgcttgac
     6781 aacgtatagc cctgaagcaa tcaaaatcaa agcaatcagt acaaagaaca agaaactacc
     6841 aataaaaaca attggaccaa gcaccataat gacctcctaa actagcattt tataaagcga
     6901 atcattttca tgaagattga tgtactgggg atcaaactct tccaagcgag aaataaactg
     6961 cgtataatct gttttatctc ctagggcaat accgattaga accggccctg ttcccttatt
     7021 ggcacgtttg atatattcaa aacgagtaat atcatcattt ggccctagaa tatcatttac
     7081 aaattcacga agagcccctg gacgttgtgg aaaattaaca acaaagtagt gtttaattcc
     7141 ctcgtagata agcgcacgct cctccatttc cggcatcctg ttgatgtcgt tattaccacc
     7201 agaaatgata caacaaatgg tttgcccctt gattttgtcc ttgactacct caagagctgc
     7261 aatcgttgct gctcctgctg gctcagcaac aatacccagc ttagaataga ggtctaaaat
     7321 agtcccggaa atccatcctt catccacacc aatcaggcga tctacgtatt tccgagccac
     7381 ttcgtaggtc gacttaccga ctttctgtac cgcaataccg tcagcaaatt tatcaatttg
     7441 gtctaattta accggacgac ctttatcaaa agccgctttc attgaccgtg ccccacttgc
     7501 ttcaacacca acaatcttaa tttcaggtgc atgttctttc agataggccg aaacgcctgc
     7561 aaccagacca cctcctccta tgggaactaa aatttggtca aaactaatgg tttgctcttc
     7621 cgcttcttcc aagatttcat aggctactgt tccctgacca gcctgcacat tttcatcatc
     7681 gaatgggtct ataaatgttt tcccactttc ctgagtataa tcttgtgctg ccttggctga
     7741 ctcatcgaag gtatcaccta cgagccgaat ctctacgtag tctcctccga aaaatttaac
     7801 ctgccctatt ttttgttgcg gagttgtaat gggcataaaa atcgtagcag gaatttgcat
     7861 ttcctgacag gtgtaggcca caccttgagc atggtttccg gccgaagcac agaccactcc
     7921 gcgtgacttc tcatcatctg tcaattgtga aatagcataa taggctcctc gaattttaaa
     7981 agagcgaaca cgctgttcat tttctctttt taaataaata gttgcaccat atttttccga
     8041 taagtatcga ctataatcca agggggtacg tactaccacg cgcttaagaa cggagtatgc
     8101 ttgttctaca ttttttgctg taatcatagt tccattaatt tctttctata tttattgtat
     8161 caatattccc tagaaattcc tagtatttaa actattatta tgtcatttta taaaaaccga
     8221 acttttttat ttttttaatg tattcatgaa tgaaatgggc aaaagagccg gaaaaaacca
     8281 gctcttttat tcattttcag agatagggga aaaaagggaa tgacatcccc tcatccgcca
     8341 acccaaacct taaatgtatt gccatcactc aaagtaaagc tgactatccc cctatctacc
     8401 atgattgtgt ttaattatag attttgaatg cgtcgtcatc attgcgtccg acaaatggca
     8461 ttgctttgcg gagttctgca cctacttttt cgatttcaag attctctgct tctttacgat
     8521 aagcttccat acgtggacga cctgctttat agtcatttac aaagtcattt gcaaatttac
     8581 cagattgaat atctgcaagg actgccttca tgttttcttt gacttggtct gtaatcacgc
     8641 gtggacctga tacatagtca ccaaattcag cagtgtttga aatagattgg cgcattttct
     8701 taaagccacc ttcgtagaca aggtcaacga tgagtttcat ctcgtggaga acttcgaaat
     8761 aagccaattc tggtgcataa ccagcttctg tcaagacttc aaaacctgcc tgcataaggg
     8821 ctgtcaaacc accgcagaga acagcttgct caccaaacaa atcttcttct gtctcttctt
     8881 tgaaagttgt ttccaaaaga ccaacacggg ctgaaccaac accttttgcc cagtccatcg
     8941 caatatgttt tgcattgcca gttgcatctt ggtaaacagc atagagggct ggtactccaa
     9001 aaccttcttc aaaggtacgg cggaccaagt gacctggacc ttttggtgca cacatgaaga
     9061 catccacatc tgctggtacc ttgataaatt caaagtgaac gttgaaacca tgggcaaaac
     9121 caagagcatt gcccgcttcc aagtttggag cgatttcttc attgtaaagg tctgcttgga
     9181 tttcgtctgg agccaagatc atgataacat ctgcttgttt tgctgcttct gctacttcaa
     9241 aagtttcaaa accgtcttct tttgccttat caaatgactt acctgcacgc acaccgatga
     9301 tgacatcgtg ccctgtatca cgcaagtttt gagcatgggc atgaccttgt gaaccataac
     9361 cgataacggc gatacgttta ccgtcaagtg ctgctactgt tacatctttt tcatattgca
     9421 ttgttactgt cataattttt tctcttttct atttttattt gtagttttgg atgttcaaag
     9481 aaaatgaagg ctagactagg cgaagataga actaacgttc atcaagcgaa gtcaacgcag
     9541 gataggtttt attatcgaaa aggattagtc tctactaaag ccagtcgcac ccgttcttgc
     9601 gatattcttg atgccataag gctgaatcac acgcaggagg gcgtcaatct tatctccatc
     9661 acctgtcatt tgaatggtaa tggagtgcgg tgccacatca acaacatttg ctctaaaggg
     9721 ttggataatc gctaaaattt ctgctcgttt tgttggcgga gctgaaattt taacaagaat
     9781 cacctcacgc tctaagtggg gaatgtccgt caaatcacgg acacggacca catcaatcaa
     9841 gcggttcagc tgtttgatga tttgttcgac ctcatcgtga ctgaccacgt ctacaatcac
     9901 cgtcacacgt gagatgccat ctacttctgt tgggccgacg gagatggact caatattgat
     9961 ttggcgacga gataggacac ctgtaaaacg attcagaaca ccagatgaat ttcgcaattt
    10021 agctgttaac attctacgca ttgaacttca cccccaacat ttctgcattg ctcttacctg
    10081 ctggcaccat cggttgaaca tgttcacgat tagaaatatg aacctctatg agcattggtt
    10141 tatcttctaa gataactttc atgtcttcac ttagagtcgc tggattgtca aaactataat
    10201 gactaattcc gtaggcctct gccaacaatt ggaaattcgg ttcatcgtca aatacagatt
    10261 ggctacgacg cttttcatag aaggattcct gccattgacg aaccattcca agagagtggt
    10321 tattgattaa tacaacctta atcggtacac catagccatt caaaatagcc aattcttggt
    10381 tggtcatttg gaagccacca tcaccgacaa agatgattac ttctttatct ggattggcta
    10441 atttggcgcc gatagctgca ggaataccaa agcccatggt ccccatgcca ccagatgtta
    10501 caagttgacg tgcatgtttg taggggtaga attgagcggc ccacatttga tgctgaccaa
    10561 cgtctgtcac gacaatggca tccccttgcg tcaactgacc aattaattca attgccacct
    10621 gtggcttaat aaccttttca tcttcatcat accaaaatgg tgcccgacgt ttgttttcca
    10681 atacttgtgc cgtccaatca gcatagcctg tttcgactgt ttctaaagca agtagagctt
    10741 tcaaggttgc cttagcatcg cctactactg gaatagccgt tttgacaact ttaccaattt
    10801 ctgcgggatc aatatcaata tgtgctaccg tcgcatttgg cgcataggtt gttggatttc
    10861 ctgttaggcg gtcatcgaat cgcgctccaa tattgataat gtaatcagcc tgatccatag
    10921 ccatattggc agcgtatgaa ccgtgcatcc cccccatagc caaggataat tcatgctcaa
    10981 tgggcatcgc acctaatcca aggagggttg aaactactgg aatacggtag cgctctgcaa
    11041 aggcaactaa ttctttattg gcatctgcat aattgacacc accaccagcc aatataactg
    11101 gtttttgagc ttggctaagc tgtttcaaaa ttttcttaac ctgcaaacca tttggttcaa
    11161 tcgttggctg ataacttggt aggtgtagag ttgggtcatg ataaaaatca acaactcttt
    11221 cttggatatc ttttggaaca tcaataacaa cgggacccgg tctccctgtt gttgcaatat
    11281 ggagagcttc tgtgatgact ctaggaatat ctgccgtatc ccgaatttgg taattgtact
    11341 tggtaatcgg cattgtcatc cccaagacat ctgcttcctg aaaagcatct ttaccgatac
    11401 cacgtgttgc gacttgacca gtaaaaacta aaagaggaac actatcaccc attgcgtccg
    11461 caattccagt aatggcattg gtagctcctg gcccagaggt tacaatggct acgcccacct
    11521 ttcctgatga tttagcgtat ccttcggcct cgtggaccgc tccttgttca tgacgagcca
    11581 aaatatgctg gattccctca tattgataga tagcgtcata tagaggcaaa actgccccac
    11641 caggataacc aaaaacaagg tctactccta gttgatgcag ggtgtccaaa atgagatagg
    11701 accccgaacg cggttggtct agttgaattt cttgcacgaa tattcctcct ctctgcaaat
    11761 ttgttatcta ctataatatc acaaggaagt agaatgtcaa ggaattttct gaaaattcct
    11821 acatttgttc gttttcttaa ctatttccta acatttctta agcaaaactt aagcaaatcc
    11881 cttgactggc ttggtttcag cttgctttca gaaaacatca gtatagtagt tccactaaaa
    11941 aaattaaatg aggtttagga tatgtattat gtagtgatac cggcttacca gccagatgaa
    12001 aaattaatcc aactattaga aataatgaaa aacaggctaa attgtcaggt tatcgttgtg
    12061 gatgatggta gtactggcgc atccaaaaat attcttgaaa tcgccaaaac ctatgcaatt
    12121 gttctccacc atgatgtcaa caaaggaaaa ggccaagcct tgcgaacagc ctttagtttc
    12181 atccaagatt tgagaaaacc aggtgttatc gtaactgctg acgcagatgg tcaacacact
    12241 gtaaatgata ttgaccgtgt cgcacgcgcc gccatgaatc taccaagccg cctgattctt
    12301 ggtgttcgtc aatttactaa agacatcccg ctccgtagcc gatttggtaa caaactaacc
    12361 agagtattgt ttagattgca aactggtgtc aatgtgtccg atacccaaac aggcttgcgt
    12421 ggattccata ctgacttaat tccattcatg ttagatattg aaggcgaccg ctatgagtac
    12481 gagatgaaca tgttaactca ggcaagtcaa cgctataaaa ttacggaagt tccaattcaa
    12541 actatttata tcgatgataa tgccagttcg cattttcgtc ccataaaaga tggactgctc
    12601 atttacaaaa atctcttcaa atttgccctg gcatcttttg gtggatttct agttgactac
    12661 ggaatatatg cacttgcctt attgctactc actagcttac caactgctct acgcttattg
    12721 gtcgcaaaca cgattgctcg catttgtagc gctacctgta attttgcact taataagaaa
    12781 ttagtcttta acaatgaaga gagcatcgca cgaacaggcg caggctactt tacactagcc
    12841 cttgtcctct tcctttgcga tacagccctc ctctatctct tccaccaaat tctaggactt
    12901 aacctctaca ttgtcaaact tgcagttgat atcttccttt tcctagcctc ttggtttgtt
    12961 caaaagaata ttatttttaa agaaaggaaa tcattttccc atgaaattgc taaaaaaaac
    13021 ctttgcctac gcagccatct tcggaatgct tttgacagga ggctttactt actccatgtt
    13081 aaaaaccttc gtgctttccg aggctatcac aacggttgct gcaagtaatg ccacagccaa
    13141 tagccagaac acagctactt catcgtcatc aactgccgca accactgcaa ctaatgtctc
    13201 tacaacggat actagttact ctgatgataa tattcagata aatttagaga cgattaccac
    13261 caataacaca actgtttacg tcgcagatat tcaggtcagc tctccagagt atttaaagac
    13321 agctctagcc caaaatacct atggcacgaa cgtaacagct aaaacttctg aaactgcgac
    13381 agcaaacaat gccattctag cggttaatgg tgactactac ggagccaatt caactggtta
    13441 tgttattaag aatggcgttc tctatcgcga cactgttcgt gacaatgctg cttacggcga
    13501 cttggcaatc tacgccgacg gctcatttga agtcatctat gaaaatgaaa tcactgccca
    13561 ggaattgatt gacaagggtg tcgtcaacct cttagcattc ggtccatcat tggtagagaa
    13621 tggcgaaatt gtcgtcgata cttcaacaga agtcggacgt gcaatgtcat ccaaccctcg
    13681 ttctgctatc gggattatcg atgaaaatca ttatatcatc gtcgtagcag atggacgtac
    13741 gtctgaaagc caaggactct ctctatcaat tggcagaagt tatgaaacaa tatggtgctc
    13801 aaactgccta caaccttgat ggcggtggtt catccactct ttactttaac ggtcaagtta
    13861 ttaacaaccc aataacaaat ggaaatacta tctcagaaag ggcggtgagt gacattgtct
    13921 acatcggtta ctaattcaac tacaagagtt tcaagaacat ttatctccta cttagcaata
    13981 acagctttcc tgtttatttt cagtcgaatc tatgaaagtc tcagctatgg cgaagtctct
    14041 gtctttatgc attatatgtt ctgtatacca ttaataggcg gagctgtgct actcggtctg
    14101 ctgcaactta tacctaccct ttcacgtctc tcttataatt tgtggaattc tggcgttgca
    14161 actattacag ctggttgtct tctccaaggc attatcaacc tatctggacg ttcaactacc
    14221 ttagatcaac cttattggta catcggcgca gccttcctag ttctatcttt agtcagtctt
    14281 tttatcaatc catccgcaaa acaatcgtcg aaaaacgacc acttaatcta caaataaatt
    14341 gtaatggcac ttaacattct taggtgcctt ttactgtaga atgaattgtt gaaaaattcg
    14401 gaaatttcca attatttatc agatctttta tcgtttcttt catatctatg atataatggg
    14461 taaaataaga tagggagaag aaaattatga ccgatacaaa caaacttaaa gactttcgac
    14521 atcgcagctc tgtttacgat tcgatggtca aatcgcccaa ccgtgctatg ttgcgtgcca
    14581 caggcatgac cgatgatagc tttgaaaagc ctattgttgg ggttatttcg acatgggctg
    14641 aaaacacacc ctgtaacatc caccttcatg actttggaaa attagccaaa gaaggggtaa
    14701 aagatgcagg tgcctggcct gtccaatacg gtaccatcac cgttgcagac gggattgcca
    14761 tgggaactcc tggtatgcgc ttctccttga cttcacgtga tatcattgca gattctattg
    14821 aggcagctat gggaggacac aacgtggatg cctttgtcgc aatcggtggc tgtgataaaa
    14881 acatgcctgg ttccatgatt gccatcgcca acatggatat ccctgctgtt tttgcctacg
    14941 gcggaaccat tgcgccaggt aatctcaatg gtaaagacat cgacttggta tccgtttttg
    15001 aaggaatcgg aaaatggaac aacggtgact tgaccgctga agaggttcgt caaatcgagt
    15061 gtaatgcctg tcctggacct ggtggttgcg gtggtatgta cactgccaat accatggcta
    15121 ctgccattga ggtaatgggc atgtctattc ctggctcttc ttctcaccct gctgaatcac
    15181 cagagaaaaa agcagatatt gaagaagcag gtcgggccgt tgtccgcatg ttagaactag
    15241 gtatcaaacc atctgacatt atgacgcgtg aagcctttga agatgccatt acagtcacca
    15301 tggcactcgg tggctccacc aacgctactc tgcacttgct ggccattgcc catgctgcta
    15361 atgttgaatt aacactggaa gatttcaatg atttccaaga acgcgtgcct cacttggcag
    15421 acctgaaacc atcaggaaaa tacgttttcc aagacctcta caatgtcggc ggcgtcccag
    15481 ctgtcatgaa atacttgctc aaaaacggct tccttcatgg cgaccgcatc acatgtacag
    15541 gtaaaaccgt tgctgaaaac ctagagaatt ttgcagactt gactccaggt caagatgtca
    15601 tcatgccact tgaaaatcca aaacgcgcag acggtccact catcatccta aaaggtaacc
    15661 ttgctcctga aggtgccgtt gccaaggttt ctggtgtaaa agtccgcaac catacaggtc
    15721 cagccaaggt ctttgactca gaagaagagg cgatcgaagc agtcttgact gacgaaatcg
    15781 tagatggcga tgtagtcgtt gtccgctatg ttggaccaaa aggcggtcct ggtatgcctg
    15841 aaatgctatc cctttcttct atgattgtcg gaaaaggcca aggtgacaag gtagcccttc
    15901 taacagacgg tcgcttctct ggtggtacct atggactggt tgttggacac atcgcacctg
    15961 aagctcagga tggtggtcca attgcctacc tccgtacagg tgatttggtg acggttgatc
    16021 aagataccaa agaaatcacc atgcacgttt ccgaccaaga aatcgaagac cgcaagaaaa
    16081 cgacggtcat tccaccactc tactctcgtg gtgtcctcgg aaaatacgcc cacactgtat
    16141 cctcggcctc taaaggagct gttacagact tctggagacc agaacgtacg gggaaaaaat
    16201 aaggaactca acgaccctgt cctcgcggca gggttttcca aaaggagaaa aaatgaaact
    16261 aaacgtcgaa ctctcccgct ttcgtgttaa agaaggcaaa accgttcaag tggatgaatg
    16321 gatggccttt ctcaacgagc acatggaaga cacccttctc actcttgaag gagagaaaat
    16381 gtatgtcgaa accatcttcc gtgaggtatt ggacggacgc gaataccttt actggtactc
    16441 tgtccaagcc gaaggaagaa ttgaagtcga agattcagaa tcctatatcg acaaaaaaca
    16501 tttagagtat tgggaagaat gcatcgaccc gagctatggt atggtcggtt tggatccgca
    16561 agttatcatg attcataaaa ctatctatga aaccatggaa gaattagaca gacaatacga
    16621 tgaaacattt aaaaaatgag ctgctccatg caactcattt tctttttatt taggactgat
    16681 accaagcgta attcgaccta agcgactaat tcttgtcatt ttccaagcag gagaccagac
    16741 aatctctacc tgtgcatcgt ttataccatc aatcgttttc agggctgcaa ccaattcacc
    16801 aggcatagta tccgcacaag aacaacctga atctgtaaag gtcataacaa ccttacaaaa
    16861 accagcttca tctaaatgaa tctcataaat caaacccaaa ttgtagatgt ctagttcaat
    16921 ttcaggatcg taaatggatt ccaaaacctc taccaactgt tctgatacag ttaacgcacg
    16981 ttcattaatc tgaatatctt ctctcatcaa gttcaaacac tcctaaaata tttataagaa
    17041 gtattgtatc ataattaaac aaaatatacc tatgctatca aatattttta tttagatttt
    17101 atagagcacg aacttctgaa tcctcacctt aaccgataga aaaaatatat ttgtgccctt
    17161 gaggcaaact atgatagaaa ccatgacctg cataaatctg aaatacttgc attgtatcac
    17221 tagactcatc tttatagata tgttgataat aaggattatc cgcaaaaact gtctttagat
    17281 agtcgttctc cacaggtctg gcagtccctt ctacacgcac tacctggata aggtaccctt
    17341 cctcagaaat agccgtcatc gcaactcttt gatcccccat caattgatca tagaaatgcg
    17401 tttctgggct tgtcataaag aaaatccctt cttcgtttgc tgcagtgata tgagcatgac
    17461 gagcatgtgg atttccatac tcatccagag tagcaaaaac tccaaccttc atatcttcca
    17521 aaatatgcat aatatctttt agttccatgg tatcacccct tgaaaatacc tattttctaa
    17581 agtatatcaa aaacaagcga aaatgttaag tggaatattg atagaaatgg tgaaagtgtt
    17641 tcatgaataa ctccctactg acggaaccat ttaagaaggc agctatgttt gcttcatttt
    17701 caaccttcaa aggttcctca acccttttga tttccaattg gtatggattt gactactgaa
    17761 ccgattatga caacaatcaa aatccgtcgt tactataaaa actacctaat tataaaaaag
    17821 cgactcttga gtgctttgaa acgttgatat ttcgcggtat attaacgttt actaaactgg
    17881 cttgctttac gtgctttctt aagacctggt tttgggtgac agaagtaaaa tgcctataaa
    17941 agattattaa atcaagcttt caaagtccat tttcatcaca tttaaccact tataactcaa
    18001 tcatacaaac aatggattac cattagatta ccaaaaatct gttttcggag cttatgtgat
    18061 tgaggaacaa cattactgac aatctctatc ttcacaattt actttattaa taaatgtaca
    18121 caattctcaa tctcagtaat caactgctct tgggtgttga aatcatcgag agcattataa
    18181 atctctctta
//
LOCUS       DASFSW010000026        17765 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.026, whole genome shotgun sequence.
ACCESSION   DASFSW010000026 DASFSW010000000
VERSION     DASFSW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.026"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..336
                     /locus_tag="U1099_000704"
     CDS             <1..336
                     /locus_tag="U1099_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEM4867040.1"
                     /translation="NLLTGTEFAPRPDEYILFLEEAEDDDYLIIARHLTTLLQAYPNP
                     QALVFGRFPKETKMTEEILLAILDKHPILKTIPVLYDLDFAHTQPLFTITIGGQVELD
                     TKAFSIRFS"
     gene            complement(371..736)
                     /locus_tag="U1099_000705"
     CDS             complement(371..736)
                     /locus_tag="U1099_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEM4867041.1"
                     /translation="MYQVIKMYGDFEPWWFLDGWEEDVTSRAAFDRYEDALNAFQKEW
                     VRLSEDFPMKKSKNGTMVAFWDESDQHWCEECDEYLQRYHSLMLVEARENLPAGFIKQ
                     PTQPRVRPCKLKQNNCVNE"
     gene            complement(885..4532)
                     /gene="rpoC"
                     /locus_tag="U1099_000706"
     CDS             complement(885..4532)
                     /gene="rpoC"
                     /locus_tag="U1099_000706"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEM4867042.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERD
                     GLFDEVIFGPTKDWECSCGKYKRIRYKGITCDRCGVEVTRAKVRRERMGHIELKAPIS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLPKEIAALKEELKTASGQKRIKAVRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMVQLDGGRFAASDLNELYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIAGNVKAAKRLIE
                     RGDDRIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMVFKDADEAVMAYRNGYVHLHTRVGIATDSLDKPWKDNQKHKIMMTTVGKIL
                     FNAIMPEGLPYLQEPNNANLTEGTPDKYFLESGSDIKAAIAELPINPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIEESHERVEQ
                     IKKQFRRGMITDDERYAAVTDEWRSAKEKLEKRLVEKQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMSAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLDIRSITDGKEMIEPLEERLQGRYTKKTVKHPE
                     TGAVIIGPNQLITEDIAREIVNAGVEQVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNSDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVIAIEEDASTRTKKVFVKGKTGEGEYVVPFTARMKVEVGDQVARGAALTEGS
                     IQPKRLLEVRDVLAVETYLLSEVQKVYRSQGVEIGDKHIEVMVRQMLRKVRVMDPGDT
                     DLLMGTLMDISDFTDANAETVIAGGVPATARPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKRDNLLGLKENVIIGKIIPAGTGMARYRNLEPQAINEVEIIEDTVAEEL
                     AAEAELEAVTE"
     gene            complement(4710..8282)
                     /gene="rpoB"
                     /locus_tag="U1099_000707"
     CDS             complement(4710..8282)
                     /gene="rpoB"
                     /locus_tag="U1099_000707"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM4867043.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLVEIQTDSFQDFLDYG
                     LKEVFEDVLPVSNFTDTMELEFVGYELKEPKYTLEEARAHDANYSAPIYVTFRLVNKE
                     TGEIKTQEVFFGEFPIMTEMGTFIINGAERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTIEKDIHKNPADSRTDEALKEIYERLRPGEPKTAESSRSLLTARFFDPRRYDLAPVG
                     RYKINKKLNLRTRLLNQTLAEHVINGETGEIVLEAGTVLSRDVLEKVEAQFDELNLVE
                     YIPNDNAVLLEPVLLQKFKIVAPKDPERVVTVIGNANPAENVRTVTPADILAEMSYFL
                     NLAEGLGRVDDIDHLGNRRIRAVGELLANQVRIGLTRMERNLRERMSVQDNEVLTPQQ
                     IINIRPVTAAIKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVRD
                     VHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFIQTPYRKIDRATGTVTNEIVW
                     LTADEEDAYIVAQSTSPLDENNRFVDKIVMGRHQGNNQEFPADSADFMDVSPKQVVAV
                     ATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAIIAQHD
                     GKVVYADADKVEVRREDGSLDVYHISKFRRSNSGTAYNQRTLVKLGDIVEKGDFIADG
                     PSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDTK
                     LGPEEITREIPNVGEDALRNLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERLL
                     HAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRANGDELQSGVNMFVRVYIAQKRKI
                     KVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHLG
                     MAARNLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGVM
                     YMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVLQ
                     EILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDNNE
                     VELRDLDEGEDDDIIHVDDLEKARAKAAADAAAAFAAEEAEGKE"
     gene            complement(8839..11262)
                     /locus_tag="U1099_000708"
     CDS             complement(8839..11262)
                     /locus_tag="U1099_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM4867044.1"
                     /translation="MATKSDKQDFKKKISALVLGDVVGVFLRTLKLLFNSVAVLVFLF
                     GLFGAGIGIGFVVSLFDDVKIPKTEELVAKVSEVSRISTVTYSDGSLVSEVNSDLLRV
                     PITSEEVSNYLKQAVIATEDETFETHNGVVPKAVLRAALGSVGLGSSSGGSTLTQQLI
                     KQQLVGDAPTFTRKANEIVSALALERNMTKEEILTIYLNVSPFGRNNQGRNIAGVEAA
                     AQGIFGKPAKDLTVPQAAFIAGLPQSPIVYSPYASDGTRKSDEDMVYGVERYQDVLFN
                     MYRASFLTKEEYETYKAYDIKQDFIAPAPVMADTKDYLYYKVMEEAQEVMFDYLVKRD
                     KVSKNDLKNDETKASYEELAKQELSQGGYTIKSTVDQGIYAAMQSVVANYGSVLDDGN
                     EYVETGSVLIDNATGAILGFVGGRDYSTNQNNHAFDTLRSPASTIKPLLAYGIAIDQG
                     LIGSASILSDYPTNFSSGQPIMYGSGRGTGMMNLQTAIDRSVNIPAFWTYKMMRNAGV
                     DAKAYMDKMNYHIPMYDIESVPLGGGVEISVLTNTNAYQTLANGGVYNKHYIVESITA
                     SDGTVVYQHEAAPVQVYSKATASIMNQLLRQVVNSGYTTTFKSRLSGLNPQAASSDFV
                     GKTGTSNEVNDVWLMLSTPRVTLGTWAGNDDNSEMYVWTGYHNNSQYVAHMVDALYNV
                     NADMFAGKFELDSSVIASNVVASTGQRAGTTQVNGRQVTVGGAMTTSYWAKNGAPVTS
                     YNFMVGGTDSDRAQAWNTIIGSQTPTSSSSTQRSSSTRSSTSSSANNTNQSSETQTAT
                     SEQANNDSP"
     gene            11416..12672
                     /locus_tag="U1099_000709"
     CDS             11416..12672
                     /locus_tag="U1099_000709"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEM4867045.1"
                     /translation="MNIFEELKARGLVFQTTDEEALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVVILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVTKIQ
                     GQLSRFLDFENGDNKAEMVNNYDWFSDISFIDFLRDVGKYYTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKYNVTLQIGGSDQWGNMTAGTELLRRKADKSGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIEKQFDAARHERLAQKILAREVVTLVHGEEAYNQALNITEQLFAGNIKNLSAKEL
                     KQGLSNVPNYAVQAEDNLNIVELLVTSGIVTSKRQAREDVQNGAIYVNGERVQDLDYT
                     LSDSDKIDGELTVIRRGKKKYSVLTY"
     gene            12730..13107
                     /locus_tag="U1099_000710"
     CDS             12730..13107
                     /locus_tag="U1099_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEM4867046.1"
                     /translation="MTCIFCHQIEEIDILYQTEYFKVVWDIDPIQTGHLLIISKEHYD
                     TLSQIPSAVRYELSDLEVFLTGKLCQTLAIDGVTTARNDRLFDAGTHFHVHLIPRFRS
                     DGFWDQISLAQVQLDLNAFLKAL"
     gene            complement(13400..13609)
                     /locus_tag="U1099_000711"
     CDS             complement(13400..13609)
                     /locus_tag="U1099_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEM4867047.1"
                     /translation="MKQTLKLKNLSCQNCVKHVTNHFLELDGVEEVKIQLEEQLAEVE
                     TTVAYDLEQYQEVLEDTIYEVEELV"
     gene            complement(13606..14052)
                     /locus_tag="U1099_000712"
     CDS             complement(13606..14052)
                     /locus_tag="U1099_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEM4867048.1"
                     /translation="MEQTISAAEWQVMRVLWAQPGATSQEIIQALQEGFDWQATTIKT
                     LLGRLRKKNYLRMAKETSKYHYYPLISEEEHLQGQVELLLATICSTKQGQLVEKLLDT
                     GIFSQKSLEKLASKISQLQRTAPEQIACRCLAGQCTCGHHAKGEKT"
     gene            complement(14152..15663)
                     /locus_tag="U1099_000713"
     CDS             complement(14152..15663)
                     /locus_tag="U1099_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEM4867049.1"
                     /translation="MKKVGLLFLSVSALLLGACGNSTASEDGKLNIVTTFYPVYEFTK
                     QVAGDEANVDLLVKAGTEVHGYEPSAKDIARIQEADAFVYENENMETWVHDVEKSLDT
                     TKVNVISATEGMLLLPGGEEEHKGHDHSGERHSHAYDPHVWLSPERAITLVENIRDSL
                     VAKYPEKKDAFETNAAAYIEKLDALDAKYSETLSAAKQKYFVTQHTAFAYLALDYGLK
                     QVSITGVAADEDPTPSRLAELTEYINKYGIKYIYFEENASKSVAETLAKETGVQLDVL
                     NPLESLTDEDMKNGKDYISVMEDNLTALEKTTSQEGSEILPEEGAETAQTVYNGYFED
                     SAVKDRTLSDYAGEWQSVYPYLLDGTLDQVWDYKAKIKGGMTAEEYKAYYDTGYKTDV
                     DQINITDNTMEFVVGDKKEKFTYKYVGYKILTYKKGNRGVRFLFEATDANAGNYKYVQ
                     FSDHNIAPVKTGHFHIYFGGESQEKLLEELENWPTYYPVGLTGLEIGQEMLAH"
     gene            complement(15673..16485)
                     /locus_tag="U1099_000714"
     CDS             complement(15673..16485)
                     /locus_tag="U1099_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM4867050.1"
                     /translation="MFDLSVFHYDFMQRAFLAIIAMSLFSPILGVFLILRRQSLMSDT
                     LSHVSLAGVAFGLVLGISPTLSTVLVVIVAAVFLEYLRTIYKNFMEIGTAILMSTGLA
                     ISLIVMNKSGGKSGLSLEQYLFGSIVTISQEQVIALFTIAVIVIVLTLLFLRPMYILT
                     FDEDTAFVDGLPVRAMSIAFNVVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKSF
                     KAVIFTGMGIGFFGMVMGLLTSYYAETPASASITLIFISIFLLVNVVQKFKK"
     gene            complement(16478..17182)
                     /locus_tag="U1099_000715"
     CDS             complement(16478..17182)
                     /locus_tag="U1099_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM4867051.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHIHYYLDSGEFVTLTGENGAAKTT
                     LIKATLGILKPKQGKVSIAEKSIKGKKLRMAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RQGWFRRLTAHDEEHVRISLESVGMWEHRDKRLGALSGGQKQRAVIARMFASDPDIFI
                     LDEPTTGMDAGTKDAFYQLMHHSAKKHGKSVLMITHDPDELNKYADRNIHLVRDQQSP
                     WRCFNVHEADEEVAHV"
     gene            complement(17183..17626)
                     /locus_tag="U1099_000716"
     CDS             complement(17183..17626)
                     /locus_tag="U1099_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEM4867052.1"
                     /translation="MNRIVLEIEKYLHEIVLSSENQLEILVGSCQSTVKLTNTQEHIL
                     MLIEKAAYTNTEIAKELNVSQAAITKATKSLVAQGLLVAVRDDKDARIVRFSLTEAAK
                     PIATEHAHHHAHTLEAYEELLEHYSLEEQESIARFLSELVEKIRK"
BASE COUNT         5058 a         4254 c         3452 g         5001 t
ORIGIN      
        1 aacctgctga ctggaacaga gtttgctccc agaccagacg aatatattct atttttagag
       61 gaggcggaag acgacgatta tctcattatc gctcgtcact tgactacact tttacaagcc
      121 tatccaaatc cacaggctct tgtcttcggt cgttttccta aagagaccaa gatgacggag
      181 gagattttgc tggcgatttt ggacaagcac cctattctca aaaccatccc agttctctac
      241 gatttggatt ttgcccatac ccagcctctg tttaccatta caatcggtgg tcaagtggag
      301 ctcgacacca aagccttttc tatcagattc agctaaaaag gattgacccg caaaggtcaa
      361 tcctctttta ttattcattt acgcaattat tttgttttaa tttgcagggg cgtacacgag
      421 gttgcgtagg ttgcttaata aaaccagccg gtaaattttc tctcgcttct accaacatca
      481 gcgaatgata ccgctgcaaa tattcatcgc attcctcgca ccaatgctgg tctgattcat
      541 cccaaaaagc caccatcgtt ccattcttac ttttcttcat tggaaaatcc tctgatagcc
      601 gtacccactc tttttgaaag gcattcagtg catcctcata gcgatcaaaa gcagccctac
      661 tggtaacatc ctcttcccaa ccatctaaga accaccaggg ctcaaaatct ccatacattt
      721 taattacttg atacataaca catacttcct ttttcgtaca cattataccg aattcgtctg
      781 atttaagaaa acattctgct tgttaattcc ttatcaagtt gtgtgagcgc tttatttctg
      841 aacgaaagaa aacacatccc tcagaaagag atgtgtctca aatcttattc agttacagct
      901 tcaagctctg cttctgctgc aagttcttca gcaactgtgt cttcaatgat ttcaacttca
      961 ttgatggctt gtggttcaag attacggtag cgggccatac ctgtacctgc tgggataatc
     1021 ttaccgataa taacgttctc tttaagaccg agaaggttgt cacgtttacc acggatagca
     1081 gcatctgtaa ggacacgtgt tgtttcctgg aaggatgcgg cagacaagaa tgagttggtt
     1141 tcaagagacg cttttgtgat acccataaga actggacgag ccgttgctgg aacaccacca
     1201 gcgatgacag tttccgcatt ggcatcagta aagtctgaga tatccatgag ggtacccatg
     1261 agcaggtctg tatctcctgg atccatgaca cgaactttac gaagcatttg acgaaccatt
     1321 acctcgatgt gcttgtcgcc gatttctaca ccctggctac ggtaaacttt ttgaacttca
     1381 gaaagaaggt aagtttcaac cgccaagaca tcacggactt caagcaagcg ttttggttgg
     1441 atagaacctt cggtaagggc ggctccacgc gcgacttggt caccaacttc aaccttcata
     1501 cgggctgtaa atggaaccac atattcgcct tcgccagttt tacccttaac aaagactttc
     1561 ttggtacgag tagaagcatc ttcttcaata gcgataactt cacctttaac ctcagtgata
     1621 accgcttccc ctttcggatt acgggcttca aagatctctt ggacacgagg aagaccctgc
     1681 gtaatatcgc tgtttgaggc tacaccaccc gtgtggaagg tacgcattgt aagctgtgta
     1741 ccaggctcac cgatcgattg ggcagcgatt gtaccaactg cttcacccac ttcaaccgca
     1801 tcacctgttg ccaagttgat accataacaa tgacggcaga caccgtgacg agtgttacat
     1861 gtaaatacgc tacggatagt tacttgttca acacctgcat tgacgatttc acgggcaata
     1921 tcttccgtaa tcaattggtt tggaccaatg ataacggcac ccgtttcagg atgtttaaca
     1981 gttttcttag tgtaacgacc ttgcaaacgc tcttcaagtg gctcgatcat ttccttgcca
     2041 tctgtgattg aacggatatc aagaccacgg tctgttccac agtcgtcttc acggataata
     2101 acgtcttggg caacgtcaac caaacgacga gtcaagtaac ctgagtcggc tgtcttcaaa
     2161 gccgtatccg tcatcccctt acgggcaccg tgagttgaga agaacatttc caaaacagaa
     2221 agaccttcac ggaagttaga caagataggc aattccatga tacgtccgtt cggagctgac
     2281 atcagaccac gcataccggc caactgggag aagttggaaa tgttaccacg ggcaccagag
     2341 tccatcatca taacgattgg attcttcgga tcttgttttt caaccaggcg tttttccaat
     2401 ttttccttag ctgaacgcca ttcatctgta acagctgcat aacgctcatc gtctgtaatc
     2461 ataccacgac ggaattgttt cttgatttgt tctacacgtt cgtgagattc ttcaatgatt
     2521 tcagccttgt tgtcgataac tgggatatcg gcaataccca ctgtcaaacc agcgagtgtt
     2581 gagtgataat aacccaagtc tttcaaacgg tcaagaaggg ctgatgtctc agttgtacgg
     2641 aaacgcttga agatttcagc gatgatgtta ccaagatttt tcttcttgaa tggtgggttg
     2701 attggcaatt ctgcaatggc agccttgata tctgaacctg attccaagaa gtatttatca
     2761 ggagttcctt ctgtcaagtt agcattgttt ggctcttgca agtatggaag accttctggc
     2821 ataatcgcgt tgaacaagat ttttccgaca gttgtcatca taatcttgtg tttttggttg
     2881 tctttccaag gtttatcaag gctatccgtt gcgataccaa cacgagtatg caagtgaaca
     2941 tagccattgc ggtaagccat aacagcctca tccgcatcct tgaagaccat accttcacct
     3001 tcacgaccag catcttccat ggtcaagtag tagttaccca aaaccatatc ctgagatggt
     3061 gttacgacag gtttaccatc tttagggtta aggatgtgtt ctgccgcaag cataaggata
     3121 cgtgcttctg cttgtgcttc ttctgacaat ggaacgtgaa tcgccatctg gtcaccgtcg
     3181 aagtcggcgt tgtaggcttc acagacaagc gggtgcaaac gaagggcttt accgtcgatc
     3241 agaactggct caaaagcctg gatacccaaa cggtgaaggg taggtgcgcg gttcaaaaga
     3301 actgggtgtt ctttgatcac ttcttccaag atatcccaga tacgatcatc cccacgttca
     3361 atcaaacgtt ttgcagcttt tacgttacca gcaatatcac gggcaacgat ttcgcgcata
     3421 acaaacggtt tgaagagctc gatagccatt tcgcgtggca caccacattg gtacatttta
     3481 agcgttggac caacagcgat aacggaacgt cctgagaagt caacacgctt accaagcaag
     3541 ttttgacgga aacgtccttg cttaccttta agcatgtggc tgagtgattt aagtggacgg
     3601 ctacctggtc ctgtaattgg acgaccacgg cgaccgttgt caatcaaagc atccacagct
     3661 tcttggagca tccgtttttc gttttgtacg atgatacctg gagcgttaag ttccaagaga
     3721 cgagccaaac ggttgttacg gttgataaca cggcggtaga gttcgttcaa gtcagacgcc
     3781 gcaaaacggc caccatccaa ctgaaccatc ggacgcaaat ctggtggaat aactggaagg
     3841 atgttgagaa tcatccactc aggcttgtta ccagatttgt agaatgcatc caatacatct
     3901 aagcgacgaa ctgctttaat gcgtttttga ccagaagctg ttttcaattc ttctttcaaa
     3961 gctgcgattt cttttggaag atcgacttgt ttcaagaggt cttggatggc ttctgcaccc
     4021 attttagcaa cgaatgaacc ataaccatat tcacgcaaac gctcacggta ttcgcgctct
     4081 gtcatgattg acttgtgctc aagcggtgta tctttaggat caatcaccac gtaagccgcg
     4141 aagtagataa cttcctcaag cgcacgtggg ctcatatcca aggtcaagcc catacgactt
     4201 ggaatacctt tgaaatacca aatgtgtgaa attggtgctt tcaactcaat gtgtcccata
     4261 cgttcacgac gtacttttgc acgagtcact tcaacaccac agcggtcaca agtaatccct
     4321 ttataacgga tacgtttgta tttaccgcat gaacactccc agtcttttgt tggaccgaag
     4381 atgacttcgt caaaaagtcc atcacgttct ggtttaagtg tacgatagtt gattgtttca
     4441 ggttttttaa cctcaccgta agaccatgaa cgaaccttac ttggtgaagc taacgtgatt
     4501 tgcatacttt taaatcgatt tacgtcaacc actaataatc cctttctttt cttgtgagag
     4561 gttggattcc cagacctcaa attcttgaac aataatcagc agaatggata gctggtaagc
     4621 tatttttcta taaatcagac ggtggaagac cgtcggccct tatctatttc agttctgagg
     4681 tttggaccaa cttgttccat ccattctaat tattctttgc cttctgcttc ttctgctgca
     4741 aaggctgcgg ctgcgtcagc tgcggctttt gcacgtgctt tttcaagatc atctacgtgg
     4801 atgatgtcat catcttcacc ttcatcaagg tcacgcaact ctacttcatt gttgtcttca
     4861 tcaaggacac gcatatccaa accaagtgat tgcaattctt tgacaagaac gcggaatgat
     4921 tctggaacac ctggttttgg aattggttta cctttggtga tggcttcata ggctttaaga
     4981 cggcctgtca catcatctga cttataagtc aagatttctt gaaggacgtt tgaagcaccg
     5041 taggcttcaa gggcccaaac ctccatctca ccgaaacgct gaccaccaaa ctgagcctta
     5101 cctccgagtg gctgttgggt aacgagtgag tatggtccga ctgagcgggc gtggagttta
     5161 tcatcaacca tgtggtgaag cttgatcatg tacatgacac cgacagatac acggttgtca
     5221 aatggctcac ctgtacgtcc atcgtaaaga atggttttgg catctgaatc catacctgct
     5281 tctttaacag ttgaccaaag atcttctgaa cttgcaccat cgaaaactgg tgttgcgata
     5341 tggatgccca agttgcgagc cgccataccc aagtgaagtt ccataacctg accgatgttc
     5401 atacgtgatg gcaccccgag tgggttcaac atgatgtcaa ctggtgttcc gtctggaaga
     5461 tatggcatat cctcaacagg tacaatacgt gaaacgacac ccttgttacc gtgacgaccg
     5521 gccatcttat ctccgacctt gatcttacgt ttttgagcga tgtaaacacg aacaaacatg
     5581 ttaacacctg attgcaattc atcaccgttg gcacgagtaa agattttcac atcacgaacg
     5641 acaccatcgg caccgtgagg tacacgaaga gaggtatcac gtacttcacg tgacttgtca
     5701 ccgaagattg cgtgcaagag acgctcttca gcagaaagat ctttttcacc ttttggtgta
     5761 actttaccaa caagaatgtc gccctcttta acttcggcac caatacggat aatacccatt
     5821 tcgtccaagt tgcgaagagc atcttcacca acgtttggaa tttcgcgagt gatttcttca
     5881 gggcctaact tggtatcgcg tgtttctgat tcgtattctt ccaagtgaac agatgtatag
     5941 acatcgtcct tcacaaggcg ttcagacatg ataaccgcat cctcgaagtt gtaaccttcc
     6001 caagtcatgt aggcaacgat agggttttga ccaagagcca tttccccatt ttccatagaa
     6061 gggccatctg cgatgaagtc gcctttttca acgatgtcgc ccaattttac aagggtacgt
     6121 tggttgtagg cagtaccaga gtttgaacga cggaatttag aaatgtggta gacatcaagc
     6181 gatccatctt cacgacgcac ttctaccttg tccgcatctg cgtaaacaac cttaccatcg
     6241 tgttgagcga taattgccgc acctgagtcg tgagcagcct ggtattccat accagtacca
     6301 acgtaaggtg cttttggatc aatcaatgga acagcctgac gttgcatgtt ggcccccatg
     6361 agggcacggt tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact
     6421 acctgcttag gtgaaacgtc catgaaatct gctgaatctg ctgggaactc ttggttgtta
     6481 ccttggtgac gtcccataac aatcttatca acgaaacggt tgttttcatc aagtggcgat
     6541 gttgattggg ctacaatata agcatcttct tcatcggctg tcaaccaaac gatctcattt
     6601 gtaactgtac cagttgcacg gtcaatcttg cggtatggtg tttggatgaa accatacttg
     6661 ttgagatgcc cataagatga caagttgttg atcaaaccga tgtttggtcc ctcaggcgtt
     6721 tcaatcggac acatacgacc atagtgagtg tagtgaacgt ctcgaacctc ataaccagca
     6781 cggtcacgag tcaaaccacc aggtcccaag gctgacaaac ggcgtttgtg agacaactca
     6841 gaaagtgggt tgtgttggtc catgaactgt gacaactgag atgaaccaaa gaattctttg
     6901 attgcggctg taacaggacg gatattgatg atttgttgtg gcgtcaatac ttcattgtct
     6961 tgaaccgaca tacgctcacg caagttacgt tccatacgag tcaaaccgat acgtacttgg
     7021 ttagcaagca attcaccaac ggcacgaata cgacggttac ccaagtggtc aatatcatct
     7081 acgcgaccaa gtccttcagc caagttgagg aagtagctca tttcagccaa gatgtccgca
     7141 ggtgttactg tacgtacgtt ttctgctgga ttagcattac caatcaccgt cacaacacgt
     7201 tctggatcct taggagctac aatcttgaat ttctgcaaaa gaactggctc aagaagaaca
     7261 gcgttgtcat ttggaatata ttctaccaag ttgagctcat cgaattgtgc ttctactttt
     7321 tcaagcacat cacgactcaa tacagtacca gcttccaaga cgatttcgcc tgtctcaccg
     7381 ttaatcacat gctcagcaag tgtttggttg agcaaacgag tacggaggtt gagtttttta
     7441 ttaatcttgt aacgaccaac aggtgccaaa tcgtaacgac gtgggtcaaa gaaacgagct
     7501 gtcaaaaggc tacgagagct ttcagctgtc tttggttcac ctggacgaag gcgctcatag
     7561 atttccttaa gcgcttcatc cgtacgagaa tctgctgggt ttttatgaat atctttttca
     7621 atggtattgc gaaccaattc gctatcaccg aagatgtcaa agatttcatc atcgcctgag
     7681 aaaccaagcg cacgaacaag cgttgtgaac ggaatcttac gcgtacggtc aatacgagtg
     7741 taggcaatgt cttttgaatc tgtttccaat tccaaccaag ctccacggtt agggataacc
     7801 gttgaaccat aacctacttt accgttctta tctactttat cattgaagta gacacctggt
     7861 gaacgaacca actgagaaac gatgatacgc tctgcaccgt tgatgatgaa ggtacccatt
     7921 tcagtcatga ttggaaactc accgaagaag acttcttgtg tcttgatctc gccagtttct
     7981 ttattcacca gacggaaagt tacatagatt ggagctgagt agttcgcatc atgcgcacgc
     8041 gcctcttcca atgtgtattt aggctctttc aattcataac caacgaattc caattccatg
     8101 gtatccgtaa agtttgaaac tggaagtaca tcttcaaaga cttctttcaa accataatct
     8161 aaaaagtctt ggaaagagtc cgtttggatt tcaaccaaat ttggtaaatc aagaacttcc
     8221 ttgattcttg aaaaactacg acgggtacgg tgcttaccgt actgaacttc atgtcctgcc
     8281 aaaagttttc tcctttgtat taagatacta agccgtaaac aactcaaaga aaaataggaa
     8341 acacgacgac ggaggcaagc ctcctaggag ctgtttatct tttttcacag agttgtaggc
     8401 gagttcaatt aaaagatacg agaccgtgaa aaccaaagtg aaaataagcg gtaagtccaa
     8461 aatctttgat tttgttgacg caccctcgtc agaacgacta ggactttctt gacaaatcgt
     8521 cgaagaaagg ccagacgttt acgacgttgg gagtgacaaa ccaaagtaaa tatttcatac
     8581 actttttcac acaggtttta gcccgagttc aattatatac agccatgtga tgaccaacat
     8641 tcatcctttt tctgaatttt tagaatcttt aagaatagat aacaattctc aaaaaaattc
     8701 ctcatagaca caaaaagagc agctaaatct tcctttgaaa aagtttgatt taactgctgc
     8761 aagccttgtt tggacaatat ttcaaacaag acacacgaca aataatttac actattatag
     8821 cacaagtact aggaaaaatc aaggtgaatc attattcgct tgctcactag ttgctgtttg
     8881 cgtttctgat gactggttcg tgttattagc tgaagaactc gtgctactcc gagtcgaact
     8941 gcttcgttgg gttgagctac ttgatgttgg cgtttgggaa ccgataattg tattccaagc
     9001 ctgtgctcgg tcactatctg tccccccaac catgaagtta tagctcgtta ctggtgcacc
     9061 atttttagcc caataactag ttgtcatcgc accaccaact gttacttggc gcccatttac
     9121 ctgtgtcgtc cctgcacgtt gacctgtaga agcaacgaca ttagaagcaa taacactact
     9181 gtctaattca aattttcctg cgaacatatc cgcattaaca ttgtaaagcg catcaaccat
     9241 atgggccaca tattgtgaat tattgtggta gcctgtccag acatacattt ctgagttatc
     9301 atcattccca gcccaggtac ctaaagttac tctaggggtt gatagcatga gccacacgtc
     9361 attaacttca ttacttgtcc ccgtcttacc gacgaaatca gatgatgctg cctgtgggtt
     9421 taaaccactc aatcgactct tgaacgtcgt agtataacca gagttgacaa cctgtctcaa
     9481 aagctggttc ataatacttg cagttgcttt agaatagacc tgaaccggag ccgcttcatg
     9541 ctgatacact actgttccat cagaggcagt aatactctca acgatgtaat gtttgttata
     9601 aacgccacca tttgccaaag tttgataggc attggtattt gtcaatactg aaatttcaac
     9661 accaccgccc aacggtacac tttcaatatc gtacatagga atatgataat tcattttatc
     9721 catgtacgct ttggcatcta cacctgcatt tctcatcatc ttataggtcc aaaaagcagg
     9781 aatgttaaca gaacggtcaa tagccgtctg taagttcatc ataccagtac cacgtcctga
     9841 accatacata atcggttgtc cacttgaaaa gttcgttgga taatcggaaa ggatgctggc
     9901 agaacctatc aaaccttgat caatcgcaat accgtaagct aacagaggct tgatggttga
     9961 cgctggcgaa cgaagagtat caaaggcatg gttattttgg ttggttgaat aatcgcgacc
    10021 acccacaaat cctaaaatgg ctcctgtggc attatcaatt aacacactac ctgtctcgac
    10081 atattcgttt ccatcatcca aaacagagcc ataatttgcc acaacagact gcatcgccgc
    10141 atagattcct tgatcaacgg tactcttaat cgtgtagcca ccttggctca attcttgctt
    10201 ggccaactcc tcataggatg cctttgtttc atcatttttc aagtcatttt tagaaacctt
    10261 atcgcgtttt accaggtaat caaacataac ctcctgcgct tcttccatga ccttgtaata
    10321 gagataatct ttcgtatccg ccattacagg agctggagca ataaaatctt gtttaatatc
    10381 gtaggcttta taggtttcgt attcttcctt ggtcaagaat gacgcccgat acatattaaa
    10441 gagaacatct tgatagcgtt caacaccgta aaccatatct tcatctgatt tacgtgttcc
    10501 atctgatgca tagggagaat aaacaatcgg actttgaggc aagcctgcaa taaaagcagc
    10561 ctgcggaaca gtcaagtctt tcgctggttt accaaaaatt ccctgagccg cagcttctac
    10621 ccctgcaata tttcgtccct gattatttct accaaatgga gaaacattta agtaaatggt
    10681 caaaatctct tccttggtca tattccgctc taatgccaaa gccgaaacaa tctcattagc
    10741 cttacgcgta aaggttggag catctccgac aagttgctgt tttatcaatt gctgcgtcaa
    10801 ggttgaacca ccgctagatg aacctagacc aactgatccc agtgccgcac gcaaaacagc
    10861 cttaggcaca accccattgt gggtttcaaa tgtttcatcc tctgtcgcaa ttacagcctg
    10921 ttttagataa ttcgagacct cctccgaagt aataggtacg cgaagcagat ctgaattaac
    10981 ttccgacacc aagctaccat ccgaataggt aacggtggaa atccgactaa cttctgaaac
    11041 cttcgctacc agttcctctg ttttgggaat cttaacgtca tcaaaaagac taaccacgaa
    11101 accaattcct atgcccgcac caaaaagacc gaataggaac accaagacag ctactgaatt
    11161 gaataataat ttcagcgtcc gaaggaaaac tcctactaca tcccctaaga ctagggcact
    11221 gattttcttt ttaaaatctt gcttatctga tttagtcgcc atattaactc cttatcgttc
    11281 cattatacca aattttgttt attttccgca atttcgtggt ataataggga ggaatttttt
    11341 aaaatgaagt tcggtccaaa aatcatataa aatggtttat ggtcctgtat attaaaccta
    11401 aaggagacag cccacatgaa catttttgaa gaactaaaag ctcgtggctt ggtctttcaa
    11461 acgacagacg aagaagcctt ggtaaaagca ttgacagaag gacaggtttc ttattacaca
    11521 ggatacgatc caacagcaga tagtttacac ttggggcact tggttgttat cttaactagc
    11581 cgtcgcttgc agttggcagg ccacaagcct tacgccctag ttggcggtgc aactggtctg
    11641 attggcgacc cttccttcaa ggatgcggag cgtagcttgc aaaccaaaga caccgtggac
    11701 ggctgggtaa caaaaatcca aggtcagctt tctcgcttct tggattttga aaatggtgac
    11761 aacaaggctg aaatggtcaa caactacgac tggttctcag acatcagctt tatcgacttc
    11821 cttcgtgatg ttggtaaata ctacacggtc aactacatga tgagcaaaga ttctgtgaaa
    11881 aaacggattg aaacagggat ttcctacacc gagtttgcct accaaatcat gcagggctat
    11941 gacttctacg agcttaatga caagtacaat gtgaccctac aaatcggtgg ttctgaccag
    12001 tggggcaaca tgacagccgg tactgaattg cttcgccgca aggctgacaa gtctggtcac
    12061 gtgatgactg ttcccctcat cactgactca acaggtaaga aatttggtaa gtctgaaggc
    12121 aacgctgttt ggttggatgc cgacaagact tctccgtatg aaatgtacca attctggctc
    12181 aatgtcatgg acgatgatgc cgttcgtttc ttgaagattt ttactttctt gtcattagat
    12241 gaaatcgctg aaatcgagaa acaatttgac gctgcccgtc atgaacgctt ggctcagaag
    12301 attttggcta gggaagtcgt gacattggtt cacggtgaag aggcttataa ccaagccctt
    12361 aacatcaccg agcaattgtt cgcaggcaat atcaaaaacc tatccgcaaa agaactcaaa
    12421 cagggcctca gcaacgttcc aaactacgct gtacaggctg aagacaacct taacatcgtg
    12481 gaacttctag ttacctctgg tattgtcact tcaaaacgcc aagctcgtga agatgtgcaa
    12541 aatggtgcca tttatgtcaa cggtgagcgt gtgcaggact tggactatac cctttctgac
    12601 agtgacaaga ttgacggcga gctgaccgtt atccgtcgtg ggaagaagaa gtattctgtg
    12661 ttgacttatt aagatttttc aaggaaatcc accatgattt ccttttgttc tatcaatagg
    12721 agaacagcta tgacctgtat tttttgccat cagattgaag aaatagatat tctctaccag
    12781 acagaatatt tcaaggtggt ctgggacatt gaccctattc aaacagggca tctactgatt
    12841 atcagcaagg aacactatga cacgctcagc caaatccctt ctgctgttcg ctatgagcta
    12901 tcggacttgg aagtcttttt gactggaaaa ctctgtcaaa cattggcaat tgacggtgtg
    12961 accacagccc gcaacgatcg cttgtttgat gctggcaccc atttccatgt ccacctgatt
    13021 ccacgctttc gatcagacgg cttctgggac caaatttcac ttgcacaagt acagcttgac
    13081 ctgaacgcct ttctcaaagc attataaaaa gcagcccaac ggctgctttt tcgcttatat
    13141 agtcgtttag tttactttta gtacaaggca acgagccgca ggcataactc aatgctttct
    13201 tgtttctagg agaccagctg atgtacttcg cttttttgtt ttcaggctca ggtacgaata
    13261 gtccaccgga ctattcgtat cccccagact gtttcaatca ggcaaggcga gttcaaacac
    13321 tccagtggag tgtttgaagt tggaaataag gaaacgacgt ttcctcgctc gtcgaagtaa
    13381 taaaagagaa actaaatgac tataccaact cttccacttc ataaatagta tcttccagca
    13441 cctcttggta ctgttccaag tcataggcta cagtggtttc cacctccgcc aactgctctt
    13501 ccagctggat tttcacctct tccaccccat ccaactccaa gaagtgattg gtcacatgct
    13561 tgacacagtt ttggcacgat aagtttttca atttcaaagt ttgcttcatg ttttttctcc
    13621 ttttgcatga tgcccacagg tacattgccc cgccaagcac cgacaagcga tttgctcggg
    13681 tgcagtcctt tgtaactgcg agattttgct ggccagtttt tctaggcttt tttgagaaaa
    13741 tatccctgtg tctagcagtt tttcaaccaa ttgcccctgt tttgtggaac atatggtagc
    13801 tagtaagagc tccacctgcc cctgcaaatg ttcctcttca ctgatcagcg gataatagtg
    13861 gtatttgctg gtttccttgg ccattctcaa atagtttttc ttccgcaacc gccccaagag
    13921 tgtcttaatg gtcgtcgcct gccaatcaaa gccttcctgc aaggcctgaa taatttcctg
    13981 agaagtcgcc ccaggttgcg cccaaagaac ccgcataacc tgccactccg cagctgaaat
    14041 cgtctgctcc acactctcac ctccagccaa tcagatttct aaatttagtt tacacttgta
    14101 gtcaaattct gtcaagaaga aagactgaac atcttgccca gtcttactct cttagtgagc
    14161 caacatttcc tgaccaattt ccaaaccagt caaaccaact ggatagtagg ttggccaatt
    14221 ttccaattct tccaagagtt tttcttggct ttctccaccg aaatagatat ggaagtgacc
    14281 cgttttaaca ggtgcaatat tgtggtcgct aaattgaaca tacttatagt taccagcatt
    14341 tgcatccgtt gcttcaaaca agaaacgaac accacgattt ccttttttgt aagtcaaaat
    14401 cttatagcct acatacttat acgtgaattt ttctttctta tctcccacta caaattccat
    14461 ggtattatct gtaatgttaa tttgatcaac atctgttttg tagccagtat cgtaataagc
    14521 cttgtactct tcagctgtca taccaccttt gatcttagcc ttgtaatccc agacctgatc
    14581 tagtgtacca tcaagcaagt atggataaac agattgccac tcacctgcat aatctgaaag
    14641 agtacgatct tttacggcac tatcttcaaa ataaccgtta taaacagttt gcgcagtctc
    14701 agcaccttct tctggcaaaa tttcagaacc ttcttgggaa gttgtttttt caagggcagt
    14761 cagattatct tccataacag aaatataatc cttaccattt ttcatatcct catccgtcaa
    14821 actttcaaga ggattaagaa catccaactg aaccccagtc tctttagcaa gtgtttctgc
    14881 aacagatttt gacgcatttt cttcaaaata gatatattta ataccatatt tattaatata
    14941 ctctgtcaat tctgctagac gtgatggagt cggatcttca tccgcagcaa cacctgtaat
    15001 agaaacttgt ttcaaaccat agtccaaagc caaatatgca aatgcagtat gttgtgtaac
    15061 aaagtatttc tgtttcgcag cagataatgt ttcagaatat ttagcatcca aagcatctaa
    15121 tttctcaata taggctgccg catttgtttc aaaggcatct ttcttttctg ggtatttagc
    15181 taccaaacta tcacgaatat tttctacaag tgtgatggca cgctcaggag acaaccatac
    15241 atgcggatca taagcatggc tatgcctctc cccactatgg tcgtgacctt tatgttcctc
    15301 ctcaccgcca ggcaaaagca acatcccctc cgtcgcacta atcacattaa cctttgttgt
    15361 gtcaagtgat ttttcaacat catgaaccca tgtttccata ttctcatttt cataaacaaa
    15421 agcatctgcc tcttggatac gagcaatatc tttagctgac ggctcataac catgaacctc
    15481 tgtcccagcc ttaaccaaca gatcaacatt tgcctcatca cctgccacct gcttggtgaa
    15541 ctcataaaca ggataaaagg tcgttacaat attcaacttc ccatcttcag aagctgtgct
    15601 attgccacaa gcccccaaaa gcaaagctga gacagacaag aataataaac caactttttt
    15661 catcttaact ccttattttt taaatttttg aacaacattc accagtaaga aaatactaat
    15721 gaaaatcaag gtaatacttg cactcgctgg ggtttctgca tagtaagaag tcagcaaccc
    15781 catgaccatt ccaaaaaagc caatgcccat cccagtaaag ataacagctt taaaactctt
    15841 accaagtcgc aatgcaatag aagcaggcaa aaccataatt gttgaaacca gcaaagcacc
    15901 tgccgcagga atcattaagg caatggcaac accagtcacc acattgaagg caatggacat
    15961 agctcgcaca ggtaacccat ctacaaatgc cgtatcctcg tcaaaggtaa gaatatacat
    16021 tggacgtaaa aacagcaatg tcagcacaat gacaatcaca gcaatcgtaa acaaagcaat
    16081 tacttgctcc tgactaatgg tcactatcga accaaacaga tattgttcca gactaagccc
    16141 tgatttccca ccagatttat tcatgacaat gagcgaaata gccaaaccag tcgacatgag
    16201 gatagccgtc ccaatttcca taaaattctt gtaaattgtc cgcaaatact ctaaaaatac
    16261 agctgccaca ataacgacaa gaacagtcga aagcgtcggt gaaatcccca ataccaaacc
    16321 aaatgcaacc cccgccaacg aaacatgact aagagtatcc gacatcaaac tctgccgtct
    16381 aagtatcaaa aagaccccca aaatgggcga gaacagactc atcgcaatga ttgccaggaa
    16441 agcacgctgc ataaagtcat aatgaaatac agatagatca aacatgggca acctcctcat
    16501 ccgcttcatg aacattgaaa caacgccaag gagactgctg atcacgaacc aagtgaatat
    16561 ttcgatcagc atacttattc agctcatcag gatcatgagt tatcatcaaa accgactttc
    16621 catgcttctt agccgaatgg tgcatgagct ggtaaaaagc atccttggtc cctgcatcca
    16681 tccctgttgt cggttcatcc agaataaaaa tatctggatc agaggcaaac atacgcgcaa
    16741 tgaccgcacg ctgcttctgc ccaccactca aagcacctaa gcgcttatcc cgatgttccc
    16801 acattccaac tgattccaaa ctaatccgaa catgttcctc atcatgggct gtcaaacgac
    16861 gaaaccaacc ttgtcgagga taacgccctg attttacaaa ttcgtaaacc gtgctcggaa
    16921 aaccagcatt aaaacttgca atttgctgag gtaaataggc cattcttagc ttcttaccct
    16981 ttatactctt ctcagcaata gaaaccttcc cttgtttggg cttcaatatc cccaaagtcg
    17041 ccttaatgag ggttgtcttg gctgcgccat tctcaccagt caaagtcaca aattccccac
    17101 tatcaagata ataatgaata tgttctaaaa ccggttcttt gtcgtagtaa aacgacaagt
    17161 cttccacagt aatatatctc atctattttc taatcttctc cactaactca cttaagaatc
    17221 gagcaattga ctcctgctct tcaagactat aatgttccaa aagctcttca taagcttcca
    17281 aggtatgcgc gtgatgatga gcatgctctg tagcaatcgg cttagcagcc tcagtcaaac
    17341 taaagcgaac aatacgagcg tccttatcat ctctaaccgc caccaataac ccctgagcaa
    17401 ctaatgactt tgtagccttc gtgatcgcag cctgactaac attcaactcc ttagcaatct
    17461 cagtattagt atatgcagcc ttttcaataa gcatcaaaat atgctcctgc gtattcgtca
    17521 acttcaccgt gctttgacaa gaaccaacca aaatctccaa ttgattctcc gaactcaaaa
    17581 caatctcgtg caggtacttc tcaatctcta atacaatacg attcataggc atccccttaa
    17641 ccttttaatt aacttgttaa gtataccaca tagaaaacaa aaaaacaagg aaaatatttg
    17701 aaagctaaat tcccaaagta agttctagtc tgactcgaaa actagaaact agactgagac
    17761 gaaaa
//
LOCUS       DASFSW010000027        17447 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.027, whole genome shotgun sequence.
ACCESSION   DASFSW010000027 DASFSW010000000
VERSION     DASFSW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.027"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            46..480
                     /locus_tag="U1099_000717"
     CDS             46..480
                     /locus_tag="U1099_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM4867053.1"
                     /translation="MEDPKVNEYLTAIFNNVLVIEETSLRGSRFNDISIKEMHTIDVI
                     GSIDGATPSDVARTLMVTLGTVTTSLNNLERKGYVERIRSTKDRRVVHLYLTKKGRLV
                     YRLHQRFHNEMVKQITDGMDETEYQVMKKGLMKLYHFLEDLK"
     gene            477..1454
                     /locus_tag="U1099_000718"
     CDS             477..1454
                     /locus_tag="U1099_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEM4867054.1"
                     /translation="MRNHAKISQVAHYLPKKIVTNDDLAQRMETSDEWIRSRTGIGQR
                     HIVTGETTSDLASQVARKLLEKSQLDASEIDFIIVATITPDASMPSTAAMVQAAIGAK
                     KAFAYDLVAACSGFVFALSTAEKFLASGVYKRGLVIGAETLSRSVDWSDRSTAVLFGD
                     GAGGVLLEACEQPTFLAEILRTDGGRGASLTAGIDQKETPFSTQSRQQPFIQMEGRAI
                     FEFATRDVTATMAELLEQADMTVDRVDYFLLHQANIRILDKMARKLGVVREKFPANMD
                     KYGNTSAASLPILLSECVESGMLRLDGSQTVLMAGFGGGLTWGTLLLQL"
     gene            1514..1738
                     /locus_tag="U1099_000719"
     CDS             1514..1738
                     /locus_tag="U1099_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM4867055.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLTTTFEDLDADSLDLFQVISE
                     IEDAFDIQIDTEEGLTTVGDLVAYVEEKTK"
     gene            1909..2874
                     /gene="fabK"
                     /locus_tag="U1099_000720"
     CDS             1909..2874
                     /gene="fabK"
                     /locus_tag="U1099_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEM4867056.1"
                     /translation="MKTKITELLDIKYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDKVKSITDKPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHAAGITVIPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMTLVRQVVEA
                     VSIPVIAAGGIADGAGAAAAFMLGAEAVQVGTRFVVATESNAHQAYKEKVLKAKDIDT
                     TVSASIVGHPVRAIKNKLSSAYAAAEKDFLAGKISADAIEELGAGALRNAVVDGDVTN
                     GSVMAGQIAGLVSKEESCEDILKDIYYGAAKVIREEASRWASVGE"
     gene            2876..3796
                     /gene="fabD"
                     /locus_tag="U1099_000721"
     CDS             2876..3796
                     /gene="fabD"
                     /locus_tag="U1099_000721"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEM4867057.1"
                     /translation="MTKIAFLFAGQGSQTVGMARDLYEYYPIVRETFDQASQILGYDI
                     RDLIDKQEDKLHQTRYTQPAILTTSVAIFRLLEEEGIQPDMVAGLSLGEYSALVASGA
                     LAFEDAIALIAKRGEYMETAAPAGTGKMVAVLNTDVNLIEEICSTVTSGIVSPANYNT
                     PAQIVIGGEVAAVDEAVELLKEAGVKRMIPLNVSGPFHTALLRPASEQLAQALEQVEF
                     ADFQVELVGNTEAKVMKKEDIKSLLTRQVMEPVRFYESIATMQEAGVTNFIEIGPGKV
                     LSGFVKKIDKTAHVVAIEDIEGLQIVLNKS"
     gene            3806..4540
                     /gene="fabG"
                     /locus_tag="U1099_000722"
     CDS             3806..4540
                     /gene="fabG"
                     /locus_tag="U1099_000722"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEM4867058.1"
                     /translation="MELTNKNVFVTGSSRGIGLAIAHKFASLGANVVLNGRGQLGQDL
                     LDSFADYGVKVLAISGDISSAEDAKRMVAEAIETLGSVDILVNNAGITKDGMALRMTE
                     EDFDTVLKVNLTGTFNMTQAVLKPMTKAREGAIINLSSVSGLIGNAGQANYSASKAGV
                     IGFTKAIAREVAGRNVRVNAIAPGFIQSDMTDVLSDKIKEAMTAQIPMKRFGATEEVA
                     DVAVFLAKQEYLTGQVIAVDGGLTMR"
     gene            4934..6169
                     /gene="fabF"
                     /locus_tag="U1099_000723"
     CDS             4934..6169
                     /gene="fabF"
                     /locus_tag="U1099_000723"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEM4867059.1"
                     /translation="MTKLNRVVVTGYGLTSPIGNTPEEFWDSLKAGKIGIGKITKFDT
                     SEYSVHNAAELKDFPFDKYFVKKDTNRYDNYSLYALYAAKEAIANAQLDTETVDSDRF
                     GVILSTGIGGILEIEEQVARMNEKGPKRIRPMALPKALPNMAAGNIAMQVGANGVCKC
                     IITACASSNDALGEAFREIKFGFQDVMLAGGAEAAITPFAIGGFQALTAMSTTEDPER
                     ASIPFDKDRNGFVMGEGSAVLVLESLEHAEARGATILAEIVGYGNTCDAYHMTSPHPE
                     GLGAIKAMKLAISEAGLEPADIDYINAHGTSTPANEKGESQAIVSVFGKNTPVSSTKS
                     FTGHLLGAAGAVEAAAVIEAMRHSYAPKTAGTTELSEDIEADVIYGQGRDMEIRHAIS
                     NTFGFGGHNSVIAFKRWEA"
     gene            6170..6646
                     /locus_tag="U1099_000724"
     CDS             6170..6646
                     /locus_tag="U1099_000724"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEM4867060.1"
                     /translation="MNITEIKDLMSQFDQSSLREFSYSNAGETLHFSKNQQATVAPSP
                     AVEAPLAPVTPASPALVPAVEMSEPAPAEAGASPVAEGAVVESPLVGVAYLSPAPDKP
                     AFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGIVTEILVANQDVVEFGQGLVRIK"
     gene            6643..7065
                     /gene="fabZ"
                     /locus_tag="U1099_000725"
     CDS             6643..7065
                     /gene="fabZ"
                     /locus_tag="U1099_000725"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEM4867061.1"
                     /translation="MIDIVKIKEALPHRYPMLLVDRVLEVSEDEIVALKNVTINEPFF
                     NGHFPDYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     IMTATFVKRRGTIAVVEAKAEVDGKLAASGVLTFAIGK"
     gene            7362..8735
                     /locus_tag="U1099_000726"
     CDS             7362..8735
                     /locus_tag="U1099_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEM4867062.1"
                     /translation="MFEKILIANRGEIAVRIIRAARELGIATVAVYSEADKEALHTML
                     ADEAVCIGPARSTDSYLNMQAVISAAVVTGAQAIHPGFGFLSENSKFATLCEEVGIKF
                     IGPSGTVMDTMGDKINARAEMIKAQVPVIPGSDGEVLTSQEALAIAEKIGYPVMLKAS
                     AGGGGKGIRKVEKAEDLVPAFESASSEAKAAFGNGAMYMERVVYPARHIEVQILADQH
                     GHVIHLGERDCSLQRNNQKVLEEAPSIAIGQTMRERIGQAAVRAAQSVGYENAGTIEF
                     LLDEAKGEFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIKIAAGQELSVRQEDVRITGH
                     AIECRINAENPAFNFAPSPGKISNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKVI
                     VHGENRFEALMKMQRALYELEIDGVVTNTDFQLDLISDKRVVAGDYDTAYLMEEFLPR
                     YQEDLKK"
     gene            8750..9616
                     /locus_tag="U1099_000727"
     CDS             8750..9616
                     /locus_tag="U1099_000727"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEM4867063.1"
                     /translation="MALFRKKDKYIRINPNRSRIESAPQAKPEVPDELFSKCPACKEI
                     LYKKDLGPEKTCQHCSYNFRITAQERLALTVDDGSFEELFTGIETKNPLDFPNYLEKL
                     AATRQKTGLDEAVLTGKATIGGQPVALGIMDSHFIMASMGTVVGEKITRLFELAIEER
                     LPVVLFTASGGARMQEGIMSLMQMAKISAAVKCHSNAGLFYLTVLTDPTTGGVTASFA
                     MEGDIILAEPQTLVGFAGRRVIESTVRENLPDDFQKAEFLQEHGFVDAIVKRQDLPAT
                     IGRLLRMHGGVR"
     gene            9613..10386
                     /locus_tag="U1099_000728"
     CDS             9613..10386
                     /locus_tag="U1099_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEM4867064.1"
                     /translation="MTSDVARMIRLARDQARLTTLDYATQLFDDFIELHGDRNFRDDG
                     AIVGGIGILAGQPVTVIGIQKGKNLQDNLKRNFGQPHPEGYRKALRFMKQAEKFGRPV
                     VTFINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     QVWMLENSMYAVLSPEGFASILWKDGSRAMEAAELMKITSYELEKLQVVDKVILENGR
                     ATKEVLADIKENLVSQLAQLQALPLDKLLENRYQRFRKY"
     gene            10450..10923
                     /locus_tag="U1099_000729"
     CDS             10450..10923
                     /locus_tag="U1099_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867065.1"
                     /translation="MLVKADLSNAEELLPIQHRAFAALYKTYQDQYNPAIETMDYFQS
                     RFARPNCSYYKIIEEEHTVGLVRTTVAEDAEQGWLGLIGIDPDHRGKGYGHKAMLELE
                     SLYPTVRRWVLCTVLQESKLVSFYEKLGYKAIKTEPEQEGMDMVYMEKWIGDSDA"
     gene            10916..11548
                     /locus_tag="U1099_000730"
     CDS             10916..11548
                     /locus_tag="U1099_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867066.1"
                     /translation="MLEQCRRYLDYQGVHYCKPEKAGALAGDMIALKNVAQAARKEFQ
                     TLSLVLAEHVSPFVPERVSQWMNQAQICRPHFWTYYRFPSASLTDSALAIRLYGKGTE
                     AGISVEVSFIERKKVETSLLQQNRVLNCSIAAPLYYWVQEGQVFYRLDGTEENRQSLL
                     QAVEAGRIRKVLVKYDVPFRTVASEEELLERLIQGFSLLEPYLKMTHLAK"
     gene            11726..11972
                     /locus_tag="U1099_000731"
                     /pseudo
     CDS             11726..11972
                     /locus_tag="U1099_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11993..12391
                     /locus_tag="U1099_000732"
     CDS             11993..12391
                     /locus_tag="U1099_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4867067.1"
                     /translation="MQLTDMLGFYLLELQGATTTANDASIIESLKGVPFGLALLTTAF
                     LPAIAEEVILRGYFFKKLFGSQAVVGIIVSSLLFGALHGPTDLASWLIYGGGGLIFCV
                     LYHKTGYLIYPIAVHFINNAWSVVALYYFQ"
     gene            complement(12471..13397)
                     /locus_tag="U1099_000733"
     CDS             complement(12471..13397)
                     /locus_tag="U1099_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4867068.1"
                     /translation="MNLSKEKLLFHHYYANWLRIYKEGAIREVTLKKYRMTLQWLIRL
                     APSLLLEDLNRVRYQELINSYAKTHERQTTMDFHHQLKGAILDAVDEGIIERDPTRKV
                     IIKGRLPRKKKTKYLNQFELHQLIQELRLGEELNWDWLILLIAKTGIRFSEALALTTQ
                     DFDFHRQLLTIDKTWNYKGDGGFLPTKNKSSIRKIQLDWQTSTQFANLLQHTQPDDLL
                     FVKKERIYNSTINAILEKRCLSANLPVVTIHALRHTHASILLFAGVSIASVARRLGHA
                     SITTTQKTYLHIIRELENQDVDLIMRSLSGLC"
     gene            13519..14094
                     /locus_tag="U1099_000734"
     CDS             13519..14094
                     /locus_tag="U1099_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEM4867069.1"
                     /translation="MKLEELALFTGGSLQVRLDTTSWEDARDYILYNQQHHQLDGYEV
                     IEEVIDSRTVTTDREVTLLEEGDLLFSLLSGKAVLVRAEHAGLLYTQNYIKIEPIAKL
                     DKAFLLYLINESSAIRRQFYQSLQGSEVMKYTVKQLKSLQLGALPPLDSQQKLGKIYL
                     DGLALRQKRQAYAQGDFLRLTYLLKEVYVHE"
     gene            14087..17293
                     /locus_tag="U1099_000735"
     CDS             14087..17293
                     /locus_tag="U1099_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEM4867070.1"
                     /translation="MNELAFETELIQYITTGTITQPEHLEGLEWSVSDSSTLYAVKSK
                     LWTYEPGIKTTEQLWENFRNILWQHNQHRLDKPLSDTEFAQVKQIISDLKTPYEAGQF
                     LYGISGVSELEITLDDGRHTFLTVFDQRKIGGGDTVYQVVNQIRRTPKIPGRRECIFD
                     TTLLINGLPIIQIEEKKDTVDVDKALNQMHQYIQERQYTDIFSTLQILVAIAPNNVRY
                     MANTTAEKFNKDFAFHWQRKSDGSKVRNWKEFADSMLSIPMAHQMATNYMILDGTPQK
                     QMLKVMRPYQVYATQAVIEGIKKVDFELGCNKVGYIWHTTGSGKTITSFKTAWLASRM
                     PKVDKVVFLVDRIALTNQTKDDYLAYDPEAGENSFGSIRETQNTTALARQLTSKDSGI
                     IVTSVQKLDTLVKRKSFKSPDKHIVFIVDEAHRSTGGDSFAGIQKAFKKAAWIGYTGT
                     PNLDDTGASLRTQDIFGPLLHAYTIKNAISDRNVLGFKVDFETTIDQEKLKTTYLPDF
                     YRQKYPKWTSAQIEEKIANLSPDDMDDAVTSSVYDENPEHVRLVVEDVFKHWANRSGS
                     GHYNALFTTHVGGGRASTPMAMMYFREFQRINQLHREQGGLVLKVGVTFALNTQNNDS
                     MVESNTGLLEAITIYNTEFGTSFGLSDVASYTEDLVSRLNRTAKDKNYLDLVIVVDQL
                     LTGFNAPELNTLYVDRTLKGASLIQAYSRTNRVEDMDKKPFGRVVNYRWPAHNEKLMN
                     QALAIYADDSAANLSEAEQKNNNEKNGLVAKPFKEVFAEVKQTVDQLRELTGDFNDIP
                     KSEKKREAMLELLRAYNQGLSKLKQYQPEEVDGVIEGFDYNQPDRFIESLGMSVEEEK
                     LLTTVLANELKRTLAKEQKVSIHQIELKMTHIKEVTIDYDKLTELVELLLNQVHEEKM
                     VEAAATRDELDRFASGLEDLNYADQIRQTGDAIYRGDFPTQDMNFAYPVKGLDSQKII
                     QEASRVYIDKKLLAFRIQWGLTEHIRNQELRELLARHRYGQNDLDDTGHLRTLIKEAS
                     RDYQTWAEGDAVKGLSKIQYRNQLRQALYDLADDLVGQ"
BASE COUNT         4973 a         3415 c         4326 g         4733 t
ORIGIN      
        1 gttttatggt ttatactttg actatcaaag taaaggaggc ctgttttgga agatccaaaa
       61 gtaaatgaat atctaacggc catttttaac aatgtgttgg tgattgaaga aacaagtctg
      121 cgtggtagcc gattcaatga tatttcaatt aaggaaatgc acacgattga tgtgatagga
      181 agtattgatg gagctactcc gagtgatgtt gctcggactt tgatggtaac cttaggtact
      241 gttacaacta gcttgaacaa tttggaaaga aaaggatatg ttgagcgtat tcgttcgacc
      301 aaagatcggc gagttgttca tctctatctg accaaaaaag gtcgactggt ctatcgtttg
      361 catcaacgat ttcacaatga aatggtgaaa cagattacag atggtatgga tgaaacagag
      421 tatcaggtaa tgaagaaagg actcatgaaa ctctatcact tcttggagga tttgaaatga
      481 gaaaccatgc taaaatcagt caggtggccc actatcttcc gaagaagatt gtgaccaatg
      541 atgatttggc tcaacgaatg gagaccagtg atgagtggat tcgtagccgt acaggtatcg
      601 gtcaacgaca cattgttact ggagagacga cgagtgattt ggctagtcaa gtggctagaa
      661 aattactgga aaagtctcag ctagatgcta gtgaaattga tttcatcatc gtagcaacga
      721 ttactccaga tgcttcaatg ccatctacgg cagctatggt ccaagcagca attggtgcga
      781 aaaaggcttt cgcctatgat cttgttgcag cttgttcagg atttgttttt gcgctatcaa
      841 cagcggaaaa attccttgct tcaggagtct ataagagagg acttgtcatt ggtgcagaga
      901 ccctatctag gtctgtcgac tggtcggatc gatcaacggc tgttttattt ggagatggtg
      961 ctggaggagt tttgctggaa gcgtgcgaac aaccgacctt tttagcagaa atcttgcgaa
     1021 cggatggcgg tcgaggtgcg agtctgacag cagggataga ccagaaagag acgccattct
     1081 cgacacaaag ccgtcaacaa ccctttatcc aaatggaggg cagagctata tttgaatttg
     1141 cgacgaggga tgttacggca accatggcag agctgctaga acaagctgat atgactgtgg
     1201 atcgtgttga ttatttcttg ctccatcaag ccaatatacg tatcctagat aagatggctc
     1261 gtaaattggg agtggtgcgt gaaaaatttc cagcaaatat ggataaatat ggcaatacca
     1321 gcgcagcaag tcttcctatt ctcttatcag aatgtgtaga gagcggaatg ttacgattgg
     1381 atggaagcca gactgttctc atggctggtt tcggtggagg cctcacatgg ggaactctac
     1441 tattacaact ttagttaatt ccttgcccta ggcaagatta aaatatttta ttttccaaaa
     1501 ggagacacat atcatggcag tatttgaaaa agtacaggaa atcatcgttg aagaattggg
     1561 taaggatgct gaagaagtaa cccttacaac aacctttgaa gatttggacg cagattcatt
     1621 ggatcttttc caagttatct ctgaaatcga agatgcattc gacatccaaa tcgacactga
     1681 agaaggtttg actactgtag gtgatttggt tgcctacgtt gaagaaaaaa caaaataatg
     1741 actgttaagg aaagtgctat ttagcatttt tcttaaatta aatactttga taatcaaaac
     1801 attgaaatat caaactattt tttgagtgag ggggcaaccc ttcacttgtt tacctaatga
     1861 tttgatattc aaatgattag gtaagcaaga aaaaaagatg agggaattat gaagacgaaa
     1921 ataacagaat tgctcgatat taagtatcca atttttcaag gtggtatggc ttgggtagct
     1981 gatggagatt tggctggtgc cgtatctaat gcaggtggtc taggaatcat cggtggagga
     2041 aatgctccca aagaggtagt caaggcaaat attgacaagg ttaaatctat cacggacaaa
     2101 cctttcggtg tcaatatcat gctgttgtct ccttttgcag atgacattgt tgacttggtc
     2161 atcgaagaag gtgtgaaagt tgtcactaca ggagcaggaa atccagggaa gtatatggag
     2221 cgtctccatg ctgcagggat tacagttatt cctgttgtac cgagtgtagc ccttgctaaa
     2281 cgcatggaaa aattaggggt agatgctgtt attgctgaag gtatggaagc cggaggccat
     2341 attggtaaat tgaccaccat gaccttggtt cgtcaggttg ttgaagccgt atctattcct
     2401 gttattgctg ctggaggtat tgcagatggt gcaggtgctg cggcagcctt tatgttggga
     2461 gcagaagcgg tgcaagttgg aactcgtttt gtagtagcta cagagtccaa tgcccatcag
     2521 gcttataagg aaaaagtttt aaaagctaag gatattgata caactgtatc tgcttctatt
     2581 gttggacatc ctgtgagagc catcaagaat aagttgtcat ctgcttatgc agctgcagag
     2641 aaagatttct tggcaggtaa aatttcggca gatgctattg aagaacttgg tgcgggagca
     2701 ctccgcaatg cagttgttga tggtgatgta acaaatggct ccgtgatggc aggtcaaatt
     2761 gctggtctgg tttctaaaga agaatcttgt gaagatattt tgaaagacat ctattatgga
     2821 gcagccaaag tgattagaga agaggcaagc cgttgggctt cggttggaga ataggatgac
     2881 aaagatagct tttctttttg caggtcaagg gtctcagaca gtggggatgg ctcgcgacct
     2941 ttacgaatat tacccgatag ttagagaaac gtttgaccag gctagtcaga ttctggggta
     3001 tgacattcgc gacctaattg acaaacaaga ggataaatta catcagactc gctataccca
     3061 accggcaatt ttgacaactt ctgtggctat ttttcgactg ctggaagaag agggaataca
     3121 acccgatatg gtagctggtc tatccttggg agaatattca gctctggtag catcgggagc
     3181 tttggccttt gaagatgcga ttgccctgat tgctaagcgt ggtgaatata tggaaacggc
     3241 tgcgccagcg gggactggta agatggttgc ggttctcaac acagatgtca acttgattga
     3301 agaaatctgt tcgactgtta cctctgggat tgtttcccca gccaactaca acacaccagc
     3361 ccaaatcgtc atcggaggcg aggtggctgc agtggatgag gcggttgaat tgctaaagga
     3421 agctggtgtc aaacggatga ttccgctcaa tgtatctggc ccctttcaca cggctctctt
     3481 gcgtccagct tcggagcaat tggctcaggc cttggagcag gtggaatttg cggacttcca
     3541 agtagagttg gtcggtaata cggaagccaa ggttatgaaa aaagaggaca tcaagtccct
     3601 cttgacccgt caagtgatgg aaccggttcg tttctatgag tcaatcgcaa ccatgcagga
     3661 agctggcgtg actaacttta ttgaaatcgg tccaggtaaa gtcttatcag gttttgtcaa
     3721 gaaaatcgat aagaccgcgc atgttgtagc aattgaagac atagaggggc tacaaatagt
     3781 attgaataaa tcatgaggag gaatcatgga attaacaaat aaaaatgttt ttgtaacagg
     3841 ttcaagccga ggaattggct tggccattgc ccataagttt gccagtcttg gtgccaatgt
     3901 ggtgctgaac ggtcgtggtc agctaggcca ggacttgctg gatagctttg cagattacgg
     3961 tgtgaaagtc ctagctattt caggagatat ttcaagcgcg gaagatgcca aacgaatggt
     4021 ggcagaagcc atcgaaacac tcggtagcgt cgatattttg gtcaacaatg cagggattac
     4081 caaggatgga atggctcttc gtatgacaga agaggatttt gatacggttc tgaaggtaaa
     4141 tcttactgga acttttaata tgacgcaggc tgttttgaaa ccaatgacaa aggctcgtga
     4201 gggtgctatt atcaatctat caagcgtatc tggcttgatt ggaaatgctg gacaggccaa
     4261 ctattctgct tctaaggcag gtgtgattgg atttactaaa gccattgccc gtgaggttgc
     4321 tgggcgaaat gttcgtgtca atgcgattgc gccgggcttt atccagtctg atatgacaga
     4381 tgttttatct gacaagatta aagaagctat gactgcacaa attccgatga aacgctttgg
     4441 tgcgacagaa gaagtagcgg atgtagcagt gtttcttgcc aagcaagaat atctaactgg
     4501 acaggtgatt gcggttgacg gcggcttgac catgcggtaa tccagtcttc cagtttatct
     4561 tttattactt cgacgagtga ggaaactacg tttccagatt tccagctggg agtgggaaag
     4621 aactcgacca agataaaaag agttcgtctt cccacccccg cacagttgat taggccagat
     4681 ttggagtgca aaacacgaac aaatctgcca atcaaccact gcgctgagat gttgacacga
     4741 actctgagta gtggtcctgg gctttttgcc cagcctcacg atttccaacc ttcaatagtc
     4801 cactggatta ttgaagctgt caacaacttg ttgaaatgaa ctttttcgta gtactaaaag
     4861 caaactgaaa gactgcaata acgcactgta aattcaatgt tgttttgtgt gttattttaa
     4921 gagaggtttg aaaatgacaa aattaaatcg tgtagtagta acaggctacg gtctgacatc
     4981 tccaatcgga aatacgccag aggagttctg ggatagtttg aaggctggga aaattgggat
     5041 cggaaagatt accaagtttg ataccagtga atattcggtc cataatgccg cggaattaaa
     5101 agattttcct tttgacaaat atttcgttaa aaaggataca aatcgctacg ataattactc
     5161 gctctatgca ctctatgcag ctaaagaagc gattgctaat gcacagctgg atacagagac
     5221 agtggatagt gaccgttttg gcgttatctt atcaacaggt atcggtggta tcttggaaat
     5281 tgaagagcaa gtggctcgta tgaatgaaaa aggtccaaaa cgcattcgtc ccatggctct
     5341 tccaaaggcc ctgccaaata tggcggctgg aaatattgct atgcaggtcg gtgcaaatgg
     5401 cgtctgcaag tgcatcatca cagcctgcgc ttcatcaaat gatgctttag gggaagcctt
     5461 ccgtgaaatc aagtttggtt tccaagatgt gatgctggct ggcggagcag aggcagccat
     5521 tactcccttt gctatcggtg gtttccaggc tttgacagct atgtcgacta ctgaggatcc
     5581 agaacgtgcg tctattccat ttgacaagga ccgcaatggt tttgtcatgg gagagggttc
     5641 cgcggtttta gtattggaaa gtttggaaca cgcagaggcg cgtggagcga cgattttggc
     5701 tgaaatcgtt ggttatggaa atacctgtga tgcttaccac atgacttctc cacatccaga
     5761 aggtctgggt gctattaagg ccatgaagtt ggccatttca gaagcaggtt tggagccagc
     5821 tgatattgac tacatcaatg cccatgggac ttcgacaccg gctaatgaaa aaggggaaag
     5881 tcaagctatc gtatctgtct tcggcaagaa cacgccagtt tcttctacca agtccttcac
     5941 tggtcacttg ttgggtgcag cgggtgccgt tgaagcggca gctgtcattg aggctatgcg
     6001 tcattcttac gcaccaaaga cagctggtac gacagaatta tctgaagata ttgaagcgga
     6061 tgtcatttat ggacaggggc gtgatatgga aatccgccat gccatttcaa atacatttgg
     6121 ctttggtggg cataattcag tcatcgcttt caaacgttgg gaggcctaag tgaatattac
     6181 agaaattaag gacctgatga gtcagtttga ccagtcaagt ttgcgagaat tttcgtatag
     6241 caatgctggg gagaccttgc atttcagtaa aaaccagcag gcgacagtgg ctccgagtcc
     6301 agcggtagaa gctccgcttg ccccagtaac tcctgcaagc ccagcccttg tgccagcagt
     6361 tgaaatgagt gagccagctc cagcagaagc aggtgccagc cccgtggcag aaggtgcggt
     6421 agtggaaagc cccttggtcg gcgtggctta tttgtcccca gcccctgaca agccagcatt
     6481 tgtagcggtc ggcgatacgg tcaaaaaagg ccagaccctc atgattatcg aagctatgaa
     6541 agttatgaac gaagtaccag ctgaccgtga cggcatagtt acagaaattt tggtggccaa
     6601 ccaagatgtt gtagaatttg gacaaggatt ggtacgcatc aaatgattga tattgtaaaa
     6661 attaaagagg ctcttcctca tcgctaccct atgctcttgg tcgatcgtgt ccttgaagta
     6721 tcggaagatg agattgtcgc cctcaaaaat gtgaccatta acgagccctt cttcaacggg
     6781 catttcccag actatcctgt tatgccaggt gttctcatca tggaggcctt ggcacaaacc
     6841 gcaggtgtct tggagttgtc taaggaagaa aacaagggca agctggtttt ttatgccggc
     6901 atggacaagg tcaaatttaa gaagcaagtt gtacctggtg accagttgat catgaccgct
     6961 acatttgtca aacgccgtgg aaccattgcg gtcgttgaag caaaggcaga ggtggatgga
     7021 aaactggctg cgtcgggggt actaaccttt gccattggaa aataaatcag ttctccattt
     7081 tatcttttat tgcgtcgacg aacgaggaaa cttcgtttcc ttatttccaa cttcaaataa
     7141 tttcatcatt atttgaactc gttttgccgt acccaagtac tgtctacaac aattgatacg
     7201 aattttattc gtctcatttc caacctccaa aggtttccca aacctttgga gcaagcacta
     7261 aaagtaaaat gaagaactgc tattgataac cattatttaa tattctcatc ttgagattgt
     7321 tataacaatt attctatgtt aaaaaggaga taggattcat catgtttgag aagattttga
     7381 ttgccaatcg tggtgagatt gcggtgcgga tcattcgtgc ggccagggaa ttagggattg
     7441 cgacggtggc tgtctattca gaggcggaca aggaagctct ccacaccatg ttggcagatg
     7501 aggctgtctg tatcggcccg gcccgttcga cagactccta tctcaatatg caggctgtta
     7561 tctcggcggc tgttgtgaca ggtgcccagg ctattcatcc tggctttgga tttttgagtg
     7621 aaaactctaa attcgccacc ctctgtgaag aggtcggtat caagtttatc ggaccttctg
     7681 ggacggtcat ggataccatg ggcgataaaa tcaatgctcg ggcagagatg attaaggctc
     7741 aagttcctgt cattcctgga tcagacggcg aagttttgac cagccaagaa gctcttgcaa
     7801 ttgctgaaaa gattggttac cctgtcatgc tcaaagcatc ggcaggaggt ggcggtaagg
     7861 ggattcgtaa ggtggaaaaa gctgaagacc tagtgcctgc ctttgaatca gcttccagcg
     7921 aagccaaggc agcctttggc aacggagcca tgtatatgga gcgtgtcgtc tatccagccc
     7981 gccacatcga agtacagatt ttggcggacc agcacggtca tgtcattcat ctgggagaac
     8041 gggattgttc ccttcaacgc aacaatcaaa aggtcttgga agaagctccg tcgattgcta
     8101 tcggccagac catgcgggaa cgtatcggtc aagcagcagt gcgggctgcc cagtcagttg
     8161 gttatgaaaa tgcggggacc attgagtttc tcttggatga agccaagggc gaattttact
     8221 tcatggaaat gaacacacgg gtgcaggtag aacatcctgt taccgaattt gtcactggtg
     8281 tggatattgt caaggaacaa atcaagattg cggctggtca ggaactcagt gtccgccaag
     8341 aagatgtgcg gattacaggt catgccattg aatgccgcat caatgctgaa aatccagcct
     8401 tcaattttgc cccaagtcca ggtaagattt ccaatctcta cctaccaagc ggtggcgttg
     8461 gtctacgtgt agattcagct gtttacccag gctacaccat tccgccttac tatgattcca
     8521 tgattgccaa ggtcatcgtg catggggaaa atcgctttga agccctgatg aagatgcaac
     8581 gggccctcta tgaattggaa attgacggag tggtgaccaa taccgacttc cagctggact
     8641 tgatttccga caagcgagtg gtggctggtg attatgacac tgcctacctc atggaagaat
     8701 ttttaccccg ctatcaagaa gatctaaaaa agtaaactag gagaatgcta tggctttgtt
     8761 tcgcaaaaag gacaaatata ttcgcatcaa tcccaatcgt tcgcggatag agtcagcacc
     8821 tcaagcaaag ccagaggtgc cagatgaact cttttcgaaa tgtcctgctt gtaaggaaat
     8881 cctctacaag aaggacttgg gaccagagaa aacctgtcaa cactgttcct ataattttcg
     8941 gattacggcc caggaacgct tggctttgac agtggatgac ggttcgtttg aggaattgtt
     9001 tacagggatt gaaaccaaaa atcccttgga cttccccaat tatttagaga aattagcagc
     9061 cacccgtcaa aaaacaggct tggatgaagc tgttttgaca ggaaaggcga caatcggcgg
     9121 tcaaccagtt gcccttggca tcatggattc gcactttatc atggcttcta tgggcacggt
     9181 ggtcggtgag aaaattaccc gcctctttga gttggcgatt gaagagcgtt tgccagttgt
     9241 cctctttacc gcatctggtg gtgctcgcat gcaggaaggc attatgagtc tgatgcaaat
     9301 ggccaagatt tctgcagcag tgaaatgcca ttccaatgct ggcctctttt acctgacggt
     9361 cttgacagac ccgacaacag gaggtgttac ggctagtttt gccatggaag gcgatattat
     9421 cctggctgag ccgcagacct tggttggttt tgcaggacga cgggttattg agtcaactgt
     9481 gcgtgaaaat ctgcctgatg atttccagaa ggctgaattt ttacaagaac acggctttgt
     9541 cgatgccatt gtcaaacgcc aggacttgcc agcgaccatt ggtcgcttgt tgagaatgca
     9601 tggaggtgtc agatgaccag tgatgtagca cgtatgatcc gtttggcacg tgaccaagct
     9661 cgcctgacga ccttggacta tgccacacag ctctttgatg actttatcga attgcacggc
     9721 gatcgcaatt tccgtgatga tggagcaatt gtcggtggaa ttggcatttt agcaggtcag
     9781 cctgtgacag ttatcggtat ccaaaaaggg aaaaatcttc aggataacct caaacgcaac
     9841 tttggtcagc cccacccaga aggctaccgc aaggcccttc gcttcatgaa acaggcggag
     9901 aagttcgggc gtcccgttgt cacctttatc aatactgctg gagcctatcc cggtgtcggt
     9961 gccgaggagc gtggacaggg tgaggccatt gcccgcaacc tcatggagat gagtgacctc
    10021 aaggtgccta ttatcgctat tatcatcggc gagggcggct ctggtggagc tctggctcta
    10081 gcggtggcag atcaggtctg gatgctggaa aactccatgt acgccgtcct cagccccgaa
    10141 gggttcgcgt ctatcctctg gaaagacggc agtcgggcca tggaggcggc agaactcatg
    10201 aagattacct cctatgagtt ggagaagctc caagtggtcg ataaagttat tttggaaaat
    10261 ggcagagcga ccaaggaagt cttggctgac atcaaggaaa acctggtcag tcagttggct
    10321 caactacaag ccttgccact tgataaattg ctcgaaaacc gctatcaacg ctttagaaaa
    10381 tactaggaag acctagtatt tttttgctag atttgataca atggataaaa tcatttcagg
    10441 aggttttcca tgttagtaaa agcagatcta tcaaacgcag aagaattgct acctattcag
    10501 caccgagcat ttgcggcttt atataaaacc tatcaggacc agtacaaccc tgccattgaa
    10561 actatggact atttccaatc acgctttgca cgaccaaatt gtagctatta caagattatc
    10621 gaggaagagc acacagtcgg actagtacgg acaacggtcg ctgaagacgc tgaacagggc
    10681 tggctaggct tgattggtat tgatccagac cacagaggaa aaggctatgg tcacaaggct
    10741 atgctcgaat tggaaagcct ttatccgaca gttagacgct gggttctctg cacggttttg
    10801 caggagtcta aattagtttc tttctacgaa aagctcggct acaaggccat caaaacagag
    10861 cctgaacaag aaggcatgga tatggtctat atggagaagt ggataggaga ttcagatgct
    10921 tgaacagtgt cgcagatacc tggattatca aggagttcat tattgcaagc ctgaaaaagc
    10981 aggagctttg gcaggcgata tgatagcctt aaaaaatgta gcacaggcag cacgcaagga
    11041 gtttcagaca ttaagtttag tcttagctga gcacgtcagt ccctttgtgc ctgagagggt
    11101 cagtcaatgg atgaatcaag cccaaatttg cagaccacat ttttggacct attaccgttt
    11161 tccctctgct agtctaacgg actcggctct tgctattcgt ttgtatggta agggaactga
    11221 agcggggatt tcagtagaag tcagttttat tgaacgaaag aaagtggaga cctctctgct
    11281 tcagcaaaat cgtgttctga actgctcgat tgcggcaccg ctttattatt gggtgcaaga
    11341 gggacaggta ttttatcgcc tggatggaac ggaggagaat agacagagcc tattacaagc
    11401 cgttgaagca ggacggattc gtaaagtatt ggtcaagtat gatgtaccgt ttagaaccgt
    11461 tgcctccgag gaggaactgc tggaacgctt gattcagggc ttttctctac tagagcccta
    11521 cttgaagatg acacacctcg ctaaataatc agtggggtga tggcaacaaa catcgtaagg
    11581 gtatttgttt tttagtgtat attgacaaat tcattgaatt tttgtaaaac tgtatcgcaa
    11641 caacagaaca gtttgattga cattacgtga aatcctataa tagattgaag gcactctata
    11701 tgatgaagcg gaagaggaga agagtatgga aaaactaggt caaagtttga aatggcttgg
    11761 tcggaagatt tggctattat taaggatttt tggtctctta gtgcttgctg tccttccaga
    11821 tctgattttg gaagggtcca aatcgcaagt caccttggta atagttggac tggtgacact
    11881 gggtcttctg gtctttttct ggtggcgggc tgagaaaaat aatctaccag tctgggacaa
    11941 aaacgttctt tcttgggacg gtctggcaat agtcatacta gcctttgcta ccatgcaact
    12001 gacagacatg cttggtttct atttgttgga attgcagggg gctacaacga cagctaacga
    12061 cgcctccatc atcgaatctt tgaagggagt tccttttggg ctggcactct tgacaacagc
    12121 atttttgcca gcaattgcag aagaggttat attgcggggt tatttcttta agaaattgtt
    12181 tgggtcgcaa gcagttgttg gaatcattgt ttcaagcctg cttttcggag cccttcatgg
    12241 accgaccgat cttgcctctt ggctgattta tggaggtgga ggtcttatct tttgtgttct
    12301 ctatcataag acgggctact tgatctatcc catcgctgtc cattttatca acaatgcctg
    12361 gtcggtagtg gcactctact attttcaata agcaacagaa aagacgattc cgagtggaat
    12421 cgtcttttgc aatgaccagt acaagcttta tcacgataga gataggaatg tcaacaaagt
    12481 cctgaaagtg agcgcataat taaatcaaca tcttggtttt ccaactcacg aatgatatga
    12541 agataggttt tctgggtcgt tgttatgctt gcatgaccaa gtcgtctagc aacacttgcg
    12601 atagagaccc cggcaaataa taaaatagat gcatgggtgt gtcgcagggc gtgaatggtg
    12661 acgacaggaa gatttgcact taggcaccgc ttttcaagaa tggcattgat ggtagagttg
    12721 taaatacgtt ctttcttcac gaaaagtagg tcatcaggtt gggtgtgctg caataagttg
    12781 gcgaattggg tgctggtttg ccagtcgagt tggatttttc gaattgaaga tttatttttt
    12841 gtaggtaaaa atccgccgtc tcctttataa ttccatgttt tatcaatggt caatagctgt
    12901 cggtgaaaat caaaatcttg agtagtgaga gcaagggctt cggagaatcg aatacctgtt
    12961 ttagcaatca ataagatgag ccaatcccaa ttcagctcct ctccgagacg taattcttgt
    13021 atcaactgat gaagttcaaa ttggttgagg tattttgtct ttttctttcg aggaagtctt
    13081 cctttgataa tcaccttacg tgtcggatct cgttcaataa ttccttcatc tacagcatct
    13141 aaaatagctc ctttcaactg gtgatgaaaa tccatggttg tctgtcgttc atgtgtttta
    13201 gcatagctgt taatcaattc ttggtaacga acccgattca aatcttccaa caacagacta
    13261 ggagctagcc tgataagcca ctgcaatgtc attctatatt tcttgagagt gacttctcga
    13321 atggctcctt ccttgtaaat ccgaagccaa tttgcataat agtgatggaa aagtagtttt
    13381 tcttttgata agttcattgt agaacctcct tttttaattt ttgttaatta cataatttat
    13441 tgacagattt ccgaggagat gatatactta ttttagcgaa aaaatgtaat tatgaaaaat
    13501 ttcaaagaaa gggaaattat gaaactagaa gaattggctc tctttacggg aggtagttta
    13561 caggttcgct tggatactac cagctgggaa gatgctagag actatatcct ctacaatcag
    13621 caacatcatc aattggatgg ttatgaggtg atagaagaag tgatagatag tcggaccgtg
    13681 acgacagata gagaagtgac cttgctagag gagggagact tactctttag tttactatcg
    13741 gggaaggctg ttttagtgag agcagagcac gcaggcttac tctatacaca gaactatatc
    13801 aaaattgagc ccattgcaaa gctggataag gccttcttac tgtatctgat caatgaatcc
    13861 tcagctatta gacggcagtt ttaccaaagc ttacagggga gtgaggtaat gaaatataca
    13921 gtcaagcaac tgaaaagttt acagctaggc gccttgcctc ctttagatag tcagcagaaa
    13981 ctaggaaaga tttatttaga tggattagcc ttacggcaga agcggcaagc ttatgcgcaa
    14041 ggagattttt tacgactaac atatttgtta aaggaggttt acgtacatga atgaattagc
    14101 atttgaaaca gaattgattc aatatattac gactgggact attacacagc cggagcacct
    14161 agaaggtcta gaatggtctg ttagtgattc ttcgaccctg tatgcagtca aatctaaact
    14221 gtggacttat gagccgggta tcaagacaac ggagcaatta tgggaaaatt ttcgtaatat
    14281 tctctggcaa cacaaccaac atcgattgga caaacccttg agcgataccg agtttgctca
    14341 ggtcaagcag attatttcag atctgaagac accttatgag gctggtcagt ttttatatgg
    14401 aattagcggt gtttccgaat tggaaattac acttgatgat ggacggcata catttttgac
    14461 agtattcgac caacgtaaga taggcggtgg ggacactgtc taccaagtgg tgaaccaaat
    14521 ccgtaggact cctaaaatcc ctggacgacg agaatgtata ttcgatacaa ccttgttgat
    14581 taatggctta cctattattc agattgaaga gaaaaaggat acggtagatg tggacaaggc
    14641 attgaaccaa atgcaccaat atatccagga acggcaatat acggatattt tttctacctt
    14701 gcagattcta gtcgctatcg cccccaacaa tgttcgctat atggccaata ccactgcgga
    14761 aaaatttaat aaggactttg ctttccactg gcagcgtaaa tctgacggta gtaaggttcg
    14821 gaattggaaa gaatttgcgg attccatgct cagcattcct atggcccatc agatggcgac
    14881 caactacatg atcttagacg ggacaccgca gaaacaaatg ctgaaagtca tgcgtcccta
    14941 tcaagtttac gccactcagg cggtcattga ggggataaaa aaagtcgatt ttgaactagg
    15001 ctgtaacaag gttggctaca tctggcatac gacaggctca gggaagacga tcactagttt
    15061 taaaacagct tggctggcaa gtcgaatgcc caaggtagac aaggttgtct ttctagtgga
    15121 tcgtatcgca ttgaccaatc agactaagga tgattatctg gcttatgatc cagaagctgg
    15181 ggagaatagt tttggcagta ttcgagaaac tcaaaatacc acagccttgg cacggcagtt
    15241 gactagtaag gatagtggga ttatcgttac ttctgtgcaa aaattggata cactggtgaa
    15301 gagaaaatcc ttcaaatcac cagataagca tatcgtgttt atcgttgacg aagctcatcg
    15361 ctcaactggt ggagatagtt ttgcagggat tcaaaaggcc tttaagaaag cggcttggat
    15421 tggctataca ggaactccca acttagatga tacaggtgct agtctgcgga cgcaagatat
    15481 ttttggccct cttcttcatg cctacactat taaaaatgcc attagtgacc gcaatgtact
    15541 agggtttaag gtagattttg agacaaccat cgatcaggaa aaactaaaaa caacttactt
    15601 accagatttt tatcggcaaa aatacccgaa gtggacaagt gcacagattg aagaaaaaat
    15661 tgccaatcta agtccagatg atatggatga tgcggtgact tctagcgtct atgatgaaaa
    15721 tccagagcat gttcgattgg tagtcgaaga tgtcttcaaa cactgggcaa atcgctccgg
    15781 cagtggccat tacaatgcct tgttcaccac ccacgtaggt ggaggcaggg cctccacacc
    15841 catggctatg atgtatttcc gagagtttca acgaatcaat caactgcatc gagagcaagg
    15901 aggtctggtc ttgaaggtgg gagtgacctt tgccttgaat actcaaaata atgatagcat
    15961 ggtcgagtca aatactggtt tgcttgaagc tattacgatc tataacacag aatttgggac
    16021 gtcctttggc ttgtcagatg ttgcttctta tacagaggac ctggttagtc gcctcaatag
    16081 aacagccaag gacaagaact acttagattt ggtgattgtc gtggatcagt tgttgacagg
    16141 tttcaatgct cctgaactca atactcttta tgttgaccga accttaaaag gagccagtct
    16201 tattcaggcc tattctcgta ccaatcgtgt ggaggatatg gataaaaaac cttttggacg
    16261 ggtggtcaat taccgctggc cagcccataa tgagaaattg atgaatcagg ccttggccat
    16321 ctatgcagat gatagtgctg ccaatttatc agaagcggaa caaaaaaata acaatgaaaa
    16381 aaatggactg gtggctaagc cttttaagga agtatttgcg gaagtcaaac aaacggtcga
    16441 ccaattgaga gagttgacgg gtgatttcaa tgatattccg aagtctgaga aaaagcgtga
    16501 ggctatgtta gaattgctcc gtgcttacaa tcaaggactg tctaagctca aacagtatca
    16561 gccagaggag gttgatgggg tgattgaagg atttgattac aatcagccgg accgctttat
    16621 tgaaagtttg ggaatgtctg tagaggaaga aaaacttttg acgacagtct tagccaatga
    16681 attgaaacga accttggcga aagagcaaaa agttagcatt caccaaattg aactcaagat
    16741 gactcacatc aaggaagtga caattgacta cgataaattg accgaactgg ttgagctctt
    16801 gctgaatcag gttcacgaag agaagatggt agaagccgca gctaccaggg atgaattgga
    16861 tagatttgca agcggtttgg aagacctcaa ctatgctgac caaatccgtc aaacagggga
    16921 tgcaatctat cgaggtgatt ttccgacaca agatatgaac tttgcttatc cagtcaaggg
    16981 gcttgatagt cagaagatta tccaagaagc ttcgcgtgtt tatattgaca agaagctcct
    17041 tgcttttcga atccaatggg gcttgacgga gcatatccgc aaccaagaac tacgagagtt
    17101 gttggcgaga catcgatatg gtcagaatga cttggatgat accggacact tacgtacctt
    17161 gataaaagaa gctagtcgcg attaccagac ctgggcagaa ggtgatgctg ttaaaggctt
    17221 gagcaagatt cagtaccgca atcagttgag acaagcacta tatgatttgg ctgacgactt
    17281 ggttggtcaa taggttgcga ttttgttgga tgatagcgta taatagaatg gaaatcgtcc
    17341 gaaaaattgc aaaaataact ttcagaaata gaacaaaaag agagtagaaa aggagggact
    17401 atgacaaaag aaaaatcaac cgtaccacga ttgcgcttcc ccggatt
//
LOCUS       DASFSW010000028        17174 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.028, whole genome shotgun sequence.
ACCESSION   DASFSW010000028 DASFSW010000000
VERSION     DASFSW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17174
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.028"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            237..968
                     /locus_tag="U1099_000736"
     CDS             237..968
                     /locus_tag="U1099_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867071.1"
                     /translation="MRESSIIKNSLFWEWRCLTTSSQFYIYLILSGMYYLLLALLNFF
                     EVGFGEYLLKGPGLDGIETISLLSYLLFQAQNIFLLQQLFSGLSTTNFNLTILRIGSI
                     KDWFLVKLVSLIFLSLLLGLFQTVPLFFIGNMDYLFFLHFFVVNSTNFILFSSLLFLL
                     FHFDFSDAVSFCITYSLLVFIIFSGEILGENTNYFVTILARVAEYEKNSNLSLFIAIF
                     FEIILSAAILSFIKIRYSLGGKIHE"
     gene            961..1602
                     /locus_tag="U1099_000737"
     CDS             961..1602
                     /locus_tag="U1099_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867072.1"
                     /translation="MNNSIIKIENLSKKFKNCDLFDNLHFECSEGEIVGITGINGSGK
                     SVLFKLIAGLMQPNSGTIKVYGEDIVGKIPSGLGALIEEPGFIPNYSGFENLEFLSTI
                     QNIIGKEEILATLHRVGLFEDKDKKVRNYSLGMKKKLGIAQAIMEEPRILLLDEPTNA
                     LDSQSVQRVYDLLKELSKEKGVTVLLASHNDSDIRHLCDKVYLLENKQCKLLK"
     gene            1857..2126
                     /gene="rpsO"
                     /locus_tag="U1099_000738"
     CDS             1857..2126
                     /gene="rpsO"
                     /locus_tag="U1099_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEM4867073.1"
                     /translation="MAISKEKKNEIMAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRRTDVNRYRELIHSLGLRR"
     gene            2429..4648
                     /gene="pnp"
                     /locus_tag="U1099_000739"
     CDS             2429..4648
                     /gene="pnp"
                     /locus_tag="U1099_000739"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEM4867074.1"
                     /translation="MSKQVFETVFAGKKLVVETGQVAKQANGAVVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGWMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDPDASAPMAAMFGSSLALAISDIPFNGPIAGVQVGYVNGELI
                     INPDKAQMEESLLDLTVAGNKDAINMVESGAKELSEDIMLEALLKGHEAIQELLDFQN
                     QIVAAVGKEKADVELLQVDPELQAEIVAAYNDDLKKAVQVEEKLAREDATNAVRETVI
                     AAYEEKYAEHEEFDRIMRDVHEILELMEHAEVRRLITEDKVRPDGRRVDEIRPLDAEV
                     DFLPNVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLGDEYKKRFLHHYNFPQYSVGS
                     TGRYGAPGRREIGHGALGERALEQVLPSLEDFPYAIRLVAEVLESNGSSSQASITAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIDGITPQILEEALAQAKKARFEILDVIEATIPEVRPDLAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEDGLVAIFSPDRDAIERTKEIIAGLVREAKV
                     DEVFQAKVVRLEKFGAFVNLFDKTDALVHVSEMAWTRVNKPEDLVEIGDIVDVKVIKI
                     DDKGRIDASMKALLPKPEGYVEPEKRERSDRPRRHKDNKEKKDNNFGEFKFHKVEKK"
     gene            4702..5319
                     /gene="cysE"
                     /locus_tag="U1099_000740"
     CDS             4702..5319
                     /gene="cysE"
                     /locus_tag="U1099_000740"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEM4867075.1"
                     /translation="MGWWKDTIEIVKEKDPAARTTLEVLLTYPGVKALAAHRLSHWMW
                     KKGFKLLARMHSQFWRFWTNIEIHPGADIASGVFIDHGAGLVIGETAIIESGVMLYHG
                     VTLGGTGKDTGKRHPTVRKGALVSAHAQVIGPIEIGENAKVGAAAVVLADVPADVTVV
                     GMPAKIVRVHGQKDEKVIHDMEDGREHYTTKLAELREASHRSSHL"
     gene            5334..6107
                     /locus_tag="U1099_000741"
     CDS             5334..6107
                     /locus_tag="U1099_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEM4867076.1"
                     /translation="MLLEEFENTSAVIEPTDMAIRGGGEVCETMIFSFNGEIVDRVRQ
                     LPESREGGYLESINGRHPWYILERDGVRVAVMLAVIGAPMAVGHLEELKACGFENFIV
                     LGSCGVLDKSLVADKIILPSSALRDEGTSYHYAPASDEISYDPALLLTMEKALDQAGI
                     EHVRAKTWTTDAFYRETSAKVKRRLAAGAMVVDMEASAIMAWANFRQAKVYQFFYTAD
                     YVDYHKNEWDARREERTADSMTFFEVAMAIARKLESTSC"
     gene            6101..6286
                     /locus_tag="U1099_000742"
     CDS             6101..6286
                     /locus_tag="U1099_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867077.1"
                     /translation="MLKKLVRQNWPYVLTAIAGTIMFILKFSQGSWQVGMIWLAATAY
                     WLVKLYQKYQVLKNTQK"
     gene            6297..7640
                     /locus_tag="U1099_000743"
     CDS             6297..7640
                     /locus_tag="U1099_000743"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEM4867078.1"
                     /translation="MIKIYDTMTRSLRDFVPLEEGKVKMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYRGYDVTYISNFTDVDDKIIKAANEAGMTTKELSDKFIAAFKEDVAK
                     LGVKPATKNPRVIDYMGEIIDFVQVLIDKGYAYEASGDVYFRVEKAENYARLANKTLS
                     DLEAGASGRVEGEGQLKEHPFDFALWKTAKPGEVSWESPWGAGRPGWHIECSVMATEI
                     LGATIDIHGGGADLEFPHHTNEIAQSECKTGQTFANYWMHNAMLNINDEKMSKSLGNF
                     LTAHDMLEQIDGQVLRFFLSTQHYRRPLNYTEKAISDAEINLKYLKNTYTQPVQNSVD
                     KSALELHLAAFEAAMDDDFNAANGITAIFDFAKWINSGNYDETVKEAFGKMLEVFGIV
                     FEEEVLDSEIEALIAERQAARANRDFATADRIRDELAAQGIKLLDTKDGVRWTRD"
     gene            7633..8031
                     /locus_tag="U1099_000744"
     CDS             7633..8031
                     /locus_tag="U1099_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEM4867079.1"
                     /translation="MTKAVDVNLINGIALAFEGDAVYSMYVRRHLIFKGLTKPNKLHG
                     EANKYVSAKAQAGLISALLEAQLLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGF
                     EAVLGYLHMTEQTERLDELVGWCIDNIERG"
     gene            8033..8887
                     /locus_tag="U1099_000745"
     CDS             8033..8887
                     /locus_tag="U1099_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM4867080.1"
                     /translation="MNEKELAGWFPQAKLSQEADAQSLALGDGRYLNLADADLTERER
                     YLLSLLSHPQEQKPSSPWQSYLHGQSSLPQAVEAIQFIHVHLWSQKDAQLDAWQTMMT
                     DLLPSQLAQVQLSSQDYLFIIEQSRWTDYQTILADTLSALEFDFGLRLTFLVGQVWSR
                     QLADRWSQLYQAEAALFAHWRAHYHQSTVLSFSQLFLWGQGRAGLEFGMIKASMLQLI
                     DQQDMQDIILALWTEGAVVTKTAQILYIHRNTLQYRLDKWQDLTGLQLKDLTDLSFCY
                     QVLLDRLF"
     gene            8997..10127
                     /gene="ugpC"
                     /locus_tag="U1099_000746"
     CDS             8997..10127
                     /gene="ugpC"
                     /locus_tag="U1099_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEM4867081.1"
                     /translation="MVQLNLKNIYKKYPNSEHYSVENFNLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGEAYIDGVLMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYSKDEIKKRVEEAAQILGLTEFLQRKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRTEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNEAGT
                     GTIGRIEQIGSPEELYNKPVNKFVASFIGSPAMNFFTVTLQEGVLVGDGFKVDLPEGR
                     RKHLEEKGYNGKSFTLGIRPEDIKASQLELDTYPSSIVEAEVVVSELLGAETMLYSKV
                     GNTEFVSRVDARDYHNPGDKVRLTFNLNKAHFFDDETDQAIV"
     gene            complement(10167..11135)
                     /locus_tag="U1099_000747"
     CDS             complement(10167..11135)
                     /locus_tag="U1099_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEM4867082.1"
                     /translation="MSLSLRTIKLIFATVLAIYLATVLGLSYTTAAGIIAILSVLDTR
                     KSSFKMARNRLFSTLLALTIAVLTFALFGFGIWTLGIYLALYVPLAYRFNWEAGIAPS
                     TVLVTHLLLEQDISLIFLGNELALFLIGAGLALLFNLYMPSQEKKIQAYHDQVEDLLK
                     QILLRFEAFLLNGDGRNEAELITQLDQTLEQALKVVYLDRHNQLFQQTNYQVHYFEMR
                     AAQNKILRTMAGNINKCLLEGRENVILSSLFERAAQQLSRENSAKELLLDIELFHATF
                     RERPLPQTREEFETRATLFQLLHDMEAFIRLKVDFYEVYKDEKDPS"
     gene            complement(11210..11599)
                     /locus_tag="U1099_000748"
     CDS             complement(11210..11599)
                     /locus_tag="U1099_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEM4867083.1"
                     /translation="MTLIFGIVAGLIAFAIGGLWYGLIFRDAWIKASGIDMAKVEADR
                     QAGKNGQKEMVISLVIEVLTAVVAIFFIKTLGVSPLHAAGGMGVIAVLASLKNYVFEQ
                     RPINLILINESYKLVCYLVVGILALFA"
     gene            11737..12465
                     /gene="rlmB"
                     /locus_tag="U1099_000749"
     CDS             11737..12465
                     /gene="rlmB"
                     /locus_tag="U1099_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEM4867084.1"
                     /translation="MEHNDIVYGVHAVVESLEANTGNKLYIQDDLRGKNVEKIKALAA
                     EKKVSISWTPKKTLSDMTEGAVHQGFVLRVSEFAYADLSVILEKAEQEDNPLILILDG
                     LTDPHNFGSILRTADATQVAGIIIPKHRAVGVTPVVAKTSTGAVAHVPIARVTNLSQT
                     LDKLKEAGFWVFGTDMNGTPSHKWNTAGKLALIIGNEGKGISSNIKKQVDEMITIPMK
                     GHVQSLNASVAAAVLMYEVFRKKI"
     gene            12466..12987
                     /locus_tag="U1099_000750"
     CDS             12466..12987
                     /locus_tag="U1099_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEM4867085.1"
                     /translation="MKEKVLIVDGYNMIAFWQATKQDFKKGDLDAARTTLLRTLSHYA
                     AFEQIEVICVFDAHHVPGLRQQYDEFKVSVIFTEEEETADSYIERLSAQLNSDRRKQV
                     SVATSDLNEQWVVFSQGALRVSARELEERTRIIKKDLDKFVDQVELYTPRLNPWSDGN
                     FQALKEMMEEMKE"
     gene            12984..13847
                     /locus_tag="U1099_000751"
     CDS             12984..13847
                     /locus_tag="U1099_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM4867086.1"
                     /translation="MTFTIVTDSTSDLPISWVQENDVTVLGLTINLDGVTYETVGENR
                     LTSADLLEKMASGSQPTTSQVNVGQFEEVFEEAAKEGHEVLYLAFSAALSGTYQSAVI
                     AREMVVDQYPDAVITIVDTKAATIGEGYLVMKAVEARAAGKTLAETKAIVEDLVPRLR
                     TYLLVDDLNHLVRGGRLSKAAALIGGLVNIKPLLALNAEGKLEAIAKIRGRKKGIKEM
                     LNLTLDNLDHSTVMVAYTGDIEAAEGVKSSLLEDDRISDVLLTELGPVIATHTGTGVL
                     AILSIGTAMRA"
     gene            14311..14757
                     /gene="rplM"
                     /locus_tag="U1099_000752"
     CDS             14311..14757
                     /gene="rplM"
                     /locus_tag="U1099_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEM4867087.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKSISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            14777..15169
                     /gene="rpsI"
                     /locus_tag="U1099_000753"
     CDS             14777..15169
                     /gene="rpsI"
                     /locus_tag="U1099_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEM4867088.1"
                     /translation="MAQAQYTGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVNGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(15278..16498)
                     /locus_tag="U1099_000754"
     CDS             complement(15278..16498)
                     /locus_tag="U1099_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4867089.1"
                     /translation="MTITKTKNGTYRLKVYIPTETRMPLGIVNNNYFDKRFKTRKEAR
                     QAEIDLLTKINQIENNEFTGIGKGDILFKDFYESIWWEAYKAGQTTSTTKPPSKSTVA
                     NTKTCFRKHILPMLGNYTVQFLNQNKQVILNLMAAKASEYANFKTLRSYVISIFDWAE
                     ELEYIESNKIAKTLRRIKSTKKIQLAEARRDEDLYLTQEQLQEWFSAFQDDLASGKIS
                     LKDYVLFYLTFFLGDRKSESYALQWKHIDFEKSQIQLLQALDRYGDVKSTKGNKKTIF
                     AISTDLLQLLQGWKSQQKQELAKFGIITNPEQFVFTYIDTKGNINKPLHADYLNNKMK
                     TIKKRHPKLTHATPHKLRHTGATLAKKAGMSLEAISEALTHSDTLTTKTYVNTSNIVP
                     LSAGHVAYQHLKNE"
     gene            complement(16510..16827)
                     /locus_tag="U1099_000755"
     CDS             complement(16510..16827)
                     /locus_tag="U1099_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM4867090.1"
                     /translation="MQVILPDDQIKQIQILISSLITSEIEKQIHHRNIDNSYLNKKQA
                     CDYLGISNNTLDEWIRKGLPSIKIGKTIRFSKQAIDSWLLSQNYKYPHFCFIMVMIFS
                     GST"
BASE COUNT         4887 a         3420 c         4036 g         4831 t
ORIGIN      
        1 cttctttttc aaaaaggaag tacagtcatg gctaatcttt tctcattttt gttaatacaa
       61 tctgttttct ggttttatcc gatcaatgca accattagtc aagtctttcc tatccatcac
      121 tatattttaa aatcagtaag ttaccacaag gagttattgt attggctaat tctcttgctt
      181 tttttcattt tgttaaacct atataaaata agaaatacag agtgggagga ataggaatga
      241 gggagagttc cattataaaa aattctttat tctgggaatg gcgttgtttg actactagct
      301 ctcagtttta tatttatcta attttatcag ggatgtatta cttattgtta gcgctattga
      361 atttttttga agtcggcttc ggtgagtatc ttctaaaagg tcctgggttg gatggcatag
      421 aaaccattag tctactttct tacctattgt ttcaagcaca gaatattttt ttgttgcaac
      481 agctattttc tggtttatca acaacaaact tcaacttaac gatactaaga attgggagta
      541 taaaagattg gtttcttgtc aagcttgtgt ccttgatatt tctatcactt ttattggggt
      601 tgtttcaaac agttcctctg ttttttatag gtaatatgga ctatttgttt ttcttgcatt
      661 ttttcgtagt taatagcact aattttattt tgttcagttc attgctcttt ttattgttcc
      721 attttgattt ttcggatgct gtctcgtttt gcattactta ttctttactc gtttttatca
      781 ttttttcagg agaaatttta ggagaaaata caaattattt cgttacgata ttggcacggg
      841 tagctgagta tgagaaaaat tcaaatttgt cacttttcat cgctatattc tttgagatta
      901 tactaagtgc ggcgatactt tcttttataa agatcagata ttctttggga ggtaaaatac
      961 atgaataata gtattataaa aattgaaaac cttagcaaaa aatttaagaa ttgtgattta
     1021 tttgataatt tacactttga gtgttcggaa ggggaaatag tagggataac tggtataaat
     1081 ggttcgggga agagtgttct gttcaaattg atagcgggcc ttatgcaacc gaattctggg
     1141 actatcaagg tgtatggaga agatattgtc ggaaagattc catctggttt aggagcatta
     1201 attgaagaac caggctttat tcccaattat agtggatttg aaaatttaga atttttatca
     1261 actatccaaa atatcattgg taaggaagaa atacttgcaa ccttacatcg tgtaggactc
     1321 tttgaagata aggataaaaa ggttaggaat tactctctcg gtatgaagaa aaaattagga
     1381 attgcacagg ctattatgga agagcctaga atccttctat tggatgagcc gacaaatgct
     1441 ttggatagtc agagcgttca gagggtgtat gacttgctaa aagaactttc aaaagaaaag
     1501 ggtgttacgg ttttattggc cagtcataat gatagtgata ttagacatct atgtgataaa
     1561 gtttatcttc tcgaaaataa gcaatgtaag ttattgaaat agaaataatt tatgaattgt
     1621 gcattcagtt ttgtacaaaa ttcgtgacta ttttgacggt acggctaatt tgtaaaggtg
     1681 aatttgaaaa agaatagaga aagtctactc ggtttattgg aagataatat cagttgacaa
     1741 aagagttatt ccttgataga ataaatgagg tccaaaaagg acaaatctag ctttttcttg
     1801 gttacaggtc gccaacctgt cactcggatt aggctcaaaa caaaggagaa catatcatgg
     1861 caatctcaaa agagaaaaaa aatgaaatca tggcacaata tgctcgtcat gaaggcgaca
     1921 caggttcagt tgaagtacaa gtagcagtac ttacttggga aatcaaccac cttaacgacc
     1981 acatcaaaca acacaaaaaa gaccacgcaa cttaccgtgg tttgatgaaa aaaatcggtc
     2041 gccgtcgtaa cttgttggca tacctacgcc gcacagacgt aaaccgttac cgtgaattga
     2101 ttcactcact cggactccgt cgttaatata ggaaactgcc atgtcttcgg acattggcag
     2161 tttttcttat gttccattat ggaagtgtag tgctcggaaa aagctgggtg ttcttcgttt
     2221 gattgaagag tatgtgattc gttcggttgt ttatatagca ggttagattt tccctcaaat
     2281 tccttcctca attccccaca ttttccgtca aatatgataa aataagaagt atgcgtggtt
     2341 ttgtgcaaaa gagagtgaaa caagcttgtt tgattgtctt ttgtagaaaa tacgcaggaa
     2401 aaatttcaga aaaaatagga gacaattcat gtcaaaacaa gtatttgaaa cggtttttgc
     2461 tggcaagaaa ttggtcgttg aaacaggcca ggttgccaaa caagccaatg gtgcggttgt
     2521 tgttcgctat ggcgactcaa cagtccttac agcggcagtt atgtccaaga aaatggcaac
     2581 gggtgatttc ttcccgctcc aagtcaacta tgaagagaag atgtacgctg cagggaaatt
     2641 cccaggtggc tggatgaagc gtgaaggccg tccgtcaacc gatgcgacct tgacagctcg
     2701 tttgattgac cgtccgatcc gtccaatgtt tgcggaaggc ttccgtaacg aagtccaagt
     2761 catcaacaca gtcctttctt acgatccaga tgcctcagct ccaatggcag ccatgtttgg
     2821 ttcttcattg gcattggcaa tctcagacat cccattcaac ggcccaatcg caggtgtcca
     2881 ggttggttat gtcaatggtg aactcatcat caaccctgac aaggctcaaa tggaagagtc
     2941 tctccttgac ttgacagtag ctggtaacaa ggatgctatc aacatggtag agtctggtgc
     3001 caaagaattg tcagaagaca tcatgttgga ggcactcctc aaaggtcatg aggctattca
     3061 agaactcctt gacttccaaa atcaaatcgt agcagcagtc ggcaaggaaa aagcagatgt
     3121 ggaacttctt caagtggacc cagaattgca ggctgagatt gtcgcagctt acaatgacga
     3181 tttgaaaaaa gcggtgcagg tagaagaaaa attggcccgt gaagatgcga ccaatgccgt
     3241 gcgtgagacc gtcatcgcag cctacgaaga aaaatatgct gaacacgaag aatttgaccg
     3301 tatcatgcgt gatgtccatg aaatcttgga actcatggag catgcagagg ttcgtcgttt
     3361 gattacagaa gacaaggttc gtccagacgg tcgtcgtgtg gatgaaattc gtccgctgga
     3421 cgcagaagtg gacttcttgc caaacgttca tggctcaggt ctctttaccc gtggtcaaac
     3481 tcaggcactt tcagtcttga ccttggcccc aatgggtgaa actcaaatca tcgacggatt
     3541 gggcgacgag tacaagaaac gcttcttgca ccattacaat ttcccacaat attcagttgg
     3601 ttcaactggt cgctatggtg caccaggtcg tcgtgaaatt ggtcacggtg ctctcggtga
     3661 gcgtgctctt gagcaagtct tgccaagctt ggaagacttc ccttacgcta tccgcttggt
     3721 agcagaagtt ttggaatcaa acggttcttc atcacaggct tctatcacag ctggtaccct
     3781 tgcccttatg gcaggtggtg tgccaatcaa ggcaccagtt gcagggattg ccatgggtct
     3841 gatttcagac ggtaccaact acaccgtctt gaccgatatt caaggtcttg aggaccactt
     3901 cggcgacatg gacttcaagg tggctggtac tcgtgacggt atcacagccc ttcaaatgga
     3961 catcaagatt gacggtatca caccgcaaat cttggaagaa gccttggcac aggctaagaa
     4021 ggcacgtttt gaaatcctcg atgtcatcga agcgacgatc ccagaagttc gtcctgactt
     4081 ggcaccaact gcaccgaaga ttgacactat caagattgat gtagataaga tcaagattgt
     4141 tatcggtaag ggtggcgaaa ccatcgataa gattattgca gaaactggcg tgaaaatcga
     4201 tattgacgaa gacggtcttg tggcgatctt ctcaccagat cgcgatgcga ttgagcggac
     4261 caaggaaatc atcgcaggtc ttgttcgtga ggcaaaagtg gacgaagtct ttcaggcaaa
     4321 agttgttcgt ttggagaaat tcggtgcctt tgttaacctc tttgacaaga cagatgccct
     4381 tgttcacgtt tctgaaatgg cttggactcg tgtcaataaa ccagaagacc tagttgaaat
     4441 cggcgatatt gtcgatgtta aagtcatcaa gattgatgat aaaggacgta ttgacgcttc
     4501 tatgaaggct cttcttccaa aaccagaagg ttatgtggag ccagaaaaac gcgagcgttc
     4561 tgacagacca cgccgtcata aagacaacaa agagaaaaaa gataacaact ttggtgaatt
     4621 caaattccac aaggttgaaa agaaataagc tgagtagaca agttcggtag cgggcttgtc
     4681 ttctcgctag aaggagtgac tatgggttgg tggaaggaca ccattgagat tgtaaaagaa
     4741 aaagatccgg cagcacggac gaccttggaa gttcttttga cctacccagg tgtcaaggcc
     4801 ttggctgccc accgtctgtc ccattggatg tggaaaaaag gctttaaatt actagctagg
     4861 atgcacagcc aattttggcg tttttggact aatattgaaa ttcatccagg ggcggatatt
     4921 gcttcaggtg tcttcatcga ccatggagct ggtttggtta tcggagaaac cgccattatt
     4981 gaatcaggtg tcatgcttta ccacggagtt acgcttggtg gaacgggcaa ggatacaggc
     5041 aagcgccacc cgactgtgcg aaaaggggct ctggtttctg cccacgctca agtcattggt
     5101 cccattgaga ttggggaaaa tgccaaagtt ggtgcagctg ccgttgtcct agccgatgtt
     5161 ccagcagatg tgacagtggt cggtatgcca gccaaaatcg tccgtgtaca cggtcaaaaa
     5221 gatgagaagg tcattcatga catggaagat ggtcgtgaac actacacgac taaattagca
     5281 gagctgagag aagctagtca ccgttcatcg cacttgtagg aaagaaggtt gacgtgctac
     5341 tagaagaatt tgaaaatacc tctgcagtca ttgaaccgac ggatatggcc attcgtggtg
     5401 gcggagaggt ttgtgagacc atgattttct ccttcaatgg ggaaattgtg gaccgtgtcc
     5461 gtcagttacc tgagagcaga gaaggtggct atttagaaag cattaatggt cgtcatcctt
     5521 ggtatatttt ggaaagagat ggggtgcgag ttgcggtcat gttggcagta atcggtgctc
     5581 ccatggcagt tggtcacttg gaggaactga aagcctgtgg ctttgagaat tttatcgttc
     5641 ttggctcttg tggggtgctg gataagtcgc ttgttgcgga taagattatc cttcctagct
     5701 ctgccctgcg ggacgaggga actagttacc actacgctcc tgccagtgat gaaatcagct
     5761 atgaccctgc tctgctatta accatggaaa aggccttgga tcaagcggga atcgagcacg
     5821 tgcgggccaa gacctggacg acggacgctt tctatcggga aacgtctgcc aaggtcaaac
     5881 gtcgcctagc cgctggtgct atggtggtcg atatggaagc ttctgctatc atggcctggg
     5941 caaactttcg tcaggccaag gtctatcaat ttttctacac ggcagactat gtggactacc
     6001 acaagaatga atgggatgcc cgtcgagagg aacggacggc tgacagtatg accttctttg
     6061 aagtggccat ggcgattgca agaaaattag aaagtacatc atgttaaaaa aattagtaag
     6121 acaaaactgg ccttatgtgt tgaccgccat tgctgggaca atcatgttta tccttaaatt
     6181 ctcccaagga agctggcagg tagggatgat ttggttggca gcgacagctt attggctagt
     6241 aaaattgtat caaaagtatc aagtcctaaa gaatactcag aagtaatgga gaaactatga
     6301 ttaaaattta cgataccatg actcgcagtc tgcgtgactt tgtgcccttg gaagagggta
     6361 aggtcaagat gtatgtctgc ggtccgacgg tttacaacta tatccatatc ggtaatgccc
     6421 gtagtgtcgt ggcttttgat accatccgcc gctattttga ataccgtggc tatgatgtga
     6481 cctacatttc aaactttacg gatgtggatg ataagattat caaggctgct aatgaagctg
     6541 gcatgacaac aaaggaactt tctgataaat ttattgcagc tttcaaggaa gatgtggcaa
     6601 aactgggtgt caagcctgct accaaaaatc ctcgtgtcat cgactacatg ggtgaaatca
     6661 tcgattttgt gcaggtcttg attgacaagg gctacgccta tgaagcttct ggagatgtct
     6721 atttccgtgt ggaaaaggcg gagaattatg ctcgtttggc caacaaaacc ctgtctgact
     6781 tggaggctgg tgcttctggt cgagttgagg gagaaggtca actgaaagaa catcctttcg
     6841 actttgccct gtggaaaact gccaagccag gtgaagtgtc ttgggaaagt ccctgggggg
     6901 caggtcgtcc aggttggcat atcgagtgtt cggttatggc gacagagatt ttgggagcta
     6961 ccattgacat tcacggtggg ggagcggact tggaattccc tcaccatacc aatgaaatcg
     7021 cccagtctga gtgcaaaaca ggtcaaacct ttgccaacta ctggatgcac aatgccatgc
     7081 tcaatatcaa cgatgagaag atgtccaagt ccttgggcaa tttcctgaca gcccatgaca
     7141 tgttggaaca aattgacggt caggtcttgc gcttcttcct atctacccag cactaccgcc
     7201 gtccactcaa ctacactgag aaggccattt cagatgcgga aatcaatcta aaatatctga
     7261 aaaatactta tacacaacct gtgcaaaact ctgtggataa gtcagctctt gaattacatc
     7321 tggcggcctt tgaagcggca atggatgatg atttcaatgc ggcaaatggg attacggcta
     7381 tctttgactt tgccaaatgg atcaactcag gcaattacga tgaaactgta aaagaagcct
     7441 ttggcaaaat gctagaagtg ttcggtattg tctttgaaga agaagtattg gacagcgaaa
     7501 ttgaagcctt gatagcagaa cgtcaagcag cgcgtgccaa tcgagacttt gcgacggctg
     7561 accgtattcg tgatgaattg gcagcccagg gcatcaagct cttggatacc aaggatggtg
     7621 tgaggtggac acgtgactaa ggcagtggat gtcaatctca tcaacggaat tgccctggct
     7681 tttgaagggg atgctgtcta ttccatgtat gtcagacggc acttgatttt caaggggctg
     7741 accaagccta acaaattgca tggcgaggct aacaaatacg tctcggccaa ggctcaggca
     7801 ggcttgattt ctgccctctt agaagctcag ctattgaccg aaaaagagga agagatctac
     7861 aagcgggggc gcaataccaa tagccacacc aaggccaaga atgcggacgt ggtcacctat
     7921 cggatgtcca caggctttga ggcggtcttg ggatacctgc acatgacaga gcagacggag
     7981 cgcctggatg aactggtagg ttggtgtatt gacaatatag aaagaggata agatgaacga
     8041 gaaggaactt gcaggttggt ttccgcaggc taagttatcg caggaagcgg acgcacagtc
     8101 gcttgcttta ggcgacggtc gctatctgaa tctggcggat gcggatttga cagagcgaga
     8161 acgctatctc ttgtccttgc tgtcccatcc tcaagagcag aaaccgtcaa gcccttggca
     8221 gtcctatcta catggacaga gctctctgcc ccaggcggtt gaagctattc aatttatcca
     8281 tgtccacctt tggtcgcaga aggacgccca gttggatgca tggcagacca tgatgaccga
     8341 cctcctaccc agtcaattgg ctcaggtaca actgtcgagt caggattacc tttttatcat
     8401 cgaacaaagt cgttggacgg actatcaaac cattctggca gacaccctat cagccttgga
     8461 gtttgacttt ggcttgcggt tgacattcct agtcggtcag gtttggtctc gtcaacttgc
     8521 agatcgttgg tctcaactct atcaagcaga agcagccctc tttgctcact ggcgagcgca
     8581 ctaccatcaa tcaaccgtcc tatcctttag tcaactcttt ctttggggac aaggcagagc
     8641 aggtttggag tttggtatga taaaagctag tatgctacag ttgattgacc agcaagatat
     8701 gcaggacatc attctagccc tgtggacaga aggagccgtg gtcaccaaga ccgctcagat
     8761 cctctatatc catcgtaata ccctccaata ccgattggac aagtggcagg atttgaccgg
     8821 gctccagctc aaagacctga ctgatttaag tttttgttat caagtattac tagataggct
     8881 tttttaatct tttgtgcaac ttgtacaaaa gattgtgttc tttttgttca ccttgaccta
     8941 gaaaaataaa acgctttcat ttataataaa actatcaaag aaagtgaggc ttgaacatgg
     9001 ttcaacttaa tttaaaaaat atttataaaa aatatccaaa tagcgagcat tattccgttg
     9061 aaaactttaa tctagatatt aaagataaag agtttattgt tttcgtgggc ccatcaggat
     9121 gcggtaaatc aactactctt cgtatgattg ctggattgga agatattaca gaaggtgaag
     9181 cttacatcga cggtgtgttg atgaacgatg tagcaccaaa agaccgtgac attgccatgg
     9241 tattccagaa ctatgccctt tacccacaca tgactgtatt tgacaacatg gcttttggtc
     9301 tgaaattgcg taaatacagc aaggatgaaa tcaaaaaacg tgtagaagaa gcagcacaaa
     9361 ttctaggctt gactgaattc ttgcaacgta aacctgctga cctttctggt ggtcaacgcc
     9421 aacgtgttgc catgggccgt gccattgtcc gcgatgcaaa agtattcttg atggatgagc
     9481 ctttgtcaaa cttggatgcc aaactgcgtg tatccatgcg tacagagatt gctaaaatcc
     9541 accgtcgtat tggtgcaaca acgatctacg taacgcacga ccaaacagaa gccatgacct
     9601 tggctgaccg tattgttatc atgtctgcga ctaagaatga agcaggtaca ggtacaatcg
     9661 gtcgtatcga acaaattggt agcccagaag agctttacaa taaacctgta aacaaatttg
     9721 ttgccagctt catcggtagc cctgcaatga acttctttac agtgactctt caagagggcg
     9781 tgcttgttgg agatggtttc aaagtagatc ttccagaagg tcgtcgcaag catttggaag
     9841 aaaaaggcta caacggcaaa tcatttacat tgggtatccg tccggaagac atcaaagctt
     9901 cacaattgga gttggacact tatccatctt ctatcgtgga agcagaagtt gtcgtttcag
     9961 agcttcttgg tgcggaaacc atgctctatt ctaaagtagg gaatacagaa tttgtatctc
    10021 gtgtagatgc gcgtgattac cacaatccag gagacaaggt tcgcttgacc ttcaacctca
    10081 acaaagctca tttctttgat gatgaaacag atcaagccat tgtttaatct tttataattt
    10141 gttcataaaa caccaaagac ccgctattat gacgggtctt tttcatcttt atagacttca
    10201 taaaaatcaa ccttgaggcg aataaaggct tccatatcat gcaggagttg gaagagggtg
    10261 gcacgggttt caaattcttc ccttgtttgt ggcagaggtc gctcgcggaa ggtggcatgg
    10321 aagagctcga tgtctaggag cagttccttg gcggagtttt ctcgactcag ttgctgggct
    10381 gctcgctcga agaggctgga caggatgaca ttttctctgc cttccagtaa acatttgttg
    10441 atatttcctg ccatggtccg caggatttta ttttgtgcgg cccgcatttc aaagtaatgg
    10501 acctggtaat tggtttgctg gaagagttgg ttgtggcggt ccagatagac gactttcaga
    10561 gcttgctcca aggtctgatc caactgcgta atcaattcag cctcgtttcg tccgtctccg
    10621 ttaagtagaa aggcttcaaa gcgaagcaga atctgcttga gcaggtcttc gacttggtcg
    10681 tggtaggcct ggattttctt ttcttgcgag ggcatataga ggttaaacag gagtgccagt
    10741 cctgccccaa tcagaaaaag cgccagctca tttcccaaaa aaatcagcga aatatcttgc
    10801 tccagcaaga ggtgggtaac gaggacggtc gagggggcga tgccagcctc ccagttgaaa
    10861 cggtaggcca gggggacgta gagggctagg taaatgccca gggtccagat gccaaagcca
    10921 aagagggcga aggtcaggac ggctatggtc agggccagga gggtggaaaa gaggcggttg
    10981 cgagccattt tgaagctgga cttgcgggtg tcgaggacgc tgagaatggc gatgatgcct
    11041 gccgctgtcg tataagacag gcccagtacg gtcgctaggt aaatagccag aacggtggca
    11101 aatatcagtt tgatggtgcg gagggagagg gacatgaggg ctcctttggt tttagtctat
    11161 ttctattgta tcataaaata aaaacctcct aaggatagga gggaattact tatgcaaata
    11221 gagccagaat accgactact aggtagcaga ctagtttgta gctttcgttg atgaggataa
    11281 ggttgatagg gcgttgctca aagacgtagt ttttcaatga agcgaggacc gcaattacgc
    11341 ccatgccacc agctgcatga agtggtgaaa caccgagggt cttgatgaag aaaatagcca
    11401 caacagcagt cagtacttca atgacaaggg aaatcaccat ttccttctgt ccatttttgc
    11461 cagcctgacg atctgcttct accttagcca tgtcaatgcc tgaagccttg atccaagcat
    11521 cgcggaaaat caaaccatac cacaaaccac caatagcaaa ggcaatcagc cccgccacaa
    11581 ttccaaaaat aagtgtcata aaatctcctt tttcgattcc gtttgtacgg acaaattgat
    11641 agaactaagg ataacataga ttgtataggc ggtcaagttt ttcagctgga acacagtgag
    11701 cgcctatttt cagtcaacct atgctataat aaaaccatgg aacataatga tatcgtatat
    11761 ggcgtacatg ctgtagtgga gagtttggag gccaacaccg gcaacaaact ttacattcag
    11821 gatgatttgc gcggaaaaaa tgtagaaaaa atcaaggcgc tggcggcaga gaagaaggtg
    11881 tcgatttctt ggacgcccaa gaagaccctg tctgacatga cagaaggtgc cgttcaccaa
    11941 ggctttgtcc tgcgggtctc tgagttcgcc tatgcggacc tgtcggtgat tttggaaaag
    12001 gcagagcagg aagacaatcc cctcattctc atcttggacg gtctgacaga cccccacaat
    12061 ttcggctcga ttttgcggac cgcagatgcc actcaggtag ctggcatcat cattcccaag
    12121 caccgagcag ttggtgtgac gcccgttgtg gcaaagacct caacaggtgc ggtggcccat
    12181 gtcccaattg cccgagtgac caatcttagc caaaccctcg acaaactcaa ggaggctggt
    12241 ttctgggtat ttggtaccga tatgaacgga accccaagcc acaagtggaa tacggctggc
    12301 aagctagctc tcattatcgg caacgaaggc aagggcatct ccagcaatat caaaaagcag
    12361 gtggatgaga tgattaccat tcctatgaag ggccatgtcc aatctctcaa tgctagcgta
    12421 gcagcagctg tactgatgta cgaagtattt cggaagaaaa tctaaatgaa agaaaaagtt
    12481 ctcattgtag atggctacaa catgattgcc ttttggcagg ccaccaagca agattttaaa
    12541 aagggagact tggatgcggc cagaacgacc ttgcttcgca ccttgtctca ctacgcagcc
    12601 tttgaacaga ttgaggtaat ttgtgtcttt gatgcccacc atgtgccagg tcttcgccag
    12661 cagtatgacg aattcaaggt atcggtcatt tttacagagg aggaagagac ggcagatagt
    12721 tatatcgagc ggctcagcgc ccagctcaat agtgaccgcc gcaagcaggt ttccgtcgca
    12781 accagcgacc tcaacgaaca atgggtggtc ttttcacaag gggccttacg ggtttcggcg
    12841 cgtgaattag aggagagaac caggattatc aagaaggact tggacaagtt tgtggaccag
    12901 gtagaactct acaccccacg actcaatccc tggtcagatg ggaattttca agccttaaaa
    12961 gaaatgatgg aggaaatgaa agagtgacat ttacaatcgt aaccgattcg acatcggatt
    13021 tgccaatcag ctgggtccaa gaaaacgatg tgaccgtgct tggtttgacc attaacctag
    13081 acggcgtgac ttatgaaaca gtcggtgaaa accgcttgac ctcagcagat ttactggaga
    13141 aaatggcttc tggcagccaa cctacgacca gtcaggtcaa tgttggtcag tttgaagaag
    13201 tttttgaaga ggcagccaag gaaggtcatg aggtacttta tctcgctttc tcagcagctc
    13261 tttcagggac ctaccagagt gcagtcattg cacgagagat ggtagtggac caatatccag
    13321 atgcagtcat caccatcgtt gatacaaagg cagcgaccat tggagaaggc tacttagtca
    13381 tgaaagccgt tgaggcacgt gctgcaggta agactctggc agaaacaaag gcaatcgtag
    13441 aagatttggt gcctcgctta cggacttacc tcttggtcga cgaccttaat catttggtgc
    13501 gaggtggacg cctgtctaaa gcggcagccc tcatcggtgg attggtcaat atcaagcctc
    13561 tccttgcact caatgcggaa gggaagttag aagccattgc caaaatccgt ggtcgtaaaa
    13621 aaggaattaa ggaaatgctg aatctgacct tggataactt ggatcattct acggtcatgg
    13681 tggcctatac gggtgatatt gaggcggcag agggcgtcaa atctagttta ttggaagacg
    13741 accgtatttc tgatgttcta ttgacagaat taggaccagt tattgccact catacaggga
    13801 ctggagtctt agctatttta tcaattggga cagcaatgag agcctagatt ctaagggata
    13861 tttcttgtaa agatgtgtct cttttgtacg atgacgactt atgtagcata gggtaatcaa
    13921 aaggagatac gataatgctc attgtcaagt tgttccgacc agaagccttg gatggctgaa
    13981 actggtgttt tacaggtctt ctggtctata cggtggatga attggtcttg gaagaatttg
    14041 ataaaacctt tccttataag gagaagctgg tcttgcctgg acaactaggg cagtagggaa
    14101 agtaaacctg ataaggcttt cattctcagc agctctgcta tctttcaaaa aataaaaaaa
    14161 gtaaagtaaa atagcttgac ggagactagt agatttgata aaatggtaga cggtattgtt
    14221 taccccattt gaaaggcccc ggaaccttcc aaatacccgc ggaccggaac atccgcccag
    14281 taaacaaaaa cgattcgtat aggagaaatc atgaacaaaa caacatttat ggctaaacca
    14341 ggccaagttg aacgtaaatg gtatgtagta gacgctactg atgtacctct tggacgcctt
    14401 tcagcagtag ttgctagcgt tcttcgcggt aaaaacaaac caactttcac acctcacact
    14461 gatactggtg acttcgttat cgttatcaac gctgagaaag tgaaattgac tggtaaaaaa
    14521 gcaactgata agatttatta cactcactca aaccacccag gcggattgaa atcaatctca
    14581 gctggtgaat tgcgttctaa aaacgctgtt cgtttgattg aaaaatcagt taaaggtatg
    14641 cttccacaca acacacttgg tcgtgcacaa ggcatgaaat tgaaagtgtt tgtgggtggc
    14701 gagcacactc acgctgcaca acaaccagaa gtacttgata tttcaggtct tatctaaggg
    14761 aaaggacgta atctaaatgg cacaagcaca atacacaggt actggtcgtc gtaaaaacgc
    14821 ggttgcacgc gtacgtttgg tcccaggtac tggtaaaatc acagtaaaca aaaaagatgt
    14881 agaagagtac atcccacacg ctgaccttcg tttggttatc aaccaaccat tcgcagtaac
    14941 ttcaactcaa ggttcatacg acgttttcgt taacgtgaac ggtggtggtt acggtggtca
    15001 atcaggtgcg atccgtcacg gtatcgcgcg tgcattgctt caagtagacc cagacttccg
    15061 cgattcattg aaacgcgcag gccttcttac acgtgacgct cgtatggttg aacgtaagaa
    15121 accaggtctt aagaaagcac gtaaagctag ccagttctct aaacgttaat tatcagcgat
    15181 atatcaacgt ttcaaagcac tcgcagggtt ttccctacgg gtgctttttt ctgtgctttt
    15241 tacaaaagtt tgccctaaaa tttgccctaa atttgcacta ctcattttta agatgttgat
    15301 aggcgacatg accagcagat agagggacaa tattagaagt gttgacatag gtcttagttg
    15361 tgagagtatc actatgagtt aatgcttctg agatagcttc taagctcatt ccagctttct
    15421 tagcaagtgt tgctccagtg tgtcggagtt tatgaggtgt ggcatgtgtt agcttaggat
    15481 gtcgcttctt aatggtcttc attttattat tgagatagtc agcatgcaaa ggcttattga
    15541 tgtttccttt ggtatcaata taagtgaaga cgaactgttc aggattcgta ataattccaa
    15601 actttgcaag ttcctgtttt tgttgtgatt tccaaccttg gagaagttga agcaagtcag
    15661 ttgaaatagc aaagattgtt ttcttgttcc ctttggttga tttcacatct ccatacctat
    15721 ctagtgcttg gagtaattga atttgggatt tttcaaaatc aatgtgtttc cactgcaaag
    15781 cataagattc agattttcgg tcgcctaaaa agaaggtaag ataaaataga acatagtcct
    15841 taagtgaaat ttttccgcta gccaaatcat cttggaaggc tgagaaccat tcctgtaatt
    15901 gctcttgtgt taggtagagg tcttcatctc gcctagcttc tgccaactgg atttttttag
    15961 ttgatttgat acgacgtaag gttttagcga ttttatttga ttctatgtac tctaattctt
    16021 ctgcccaatc aaagatggat atgacatagc tacgtaaagt tttgaagttc gcatattcac
    16081 ttgcttttgc tgccattaaa tttagaataa cctgcttgtt ctgattgaga aattgaacag
    16141 tataattacc taacataggt aaaatatgtt ttctgaaaca agtttttgta ttagctaccg
    16201 ttgatttgct aggtggttta gttgttgaag tagtctgtcc tgctttgtag gcttcccacc
    16261 aaatagattc gtagaaatct ttgaatagaa tgtctccctt acctatccct gtaaactcgt
    16321 tgttctcaat ctgattgatt tttgttaaaa ggtctatctc tgcttgccgt gcctcctttc
    16381 tggtcttgaa gcgcttatca aaatagttat tattgacgat accaagtggc atccgtgttt
    16441 ccgttggaat atacactttt aagcgatatg ttccattttt tgtcttagtg atagtcatga
    16501 tattctcctt tatgtcgaac cagaaaatat catgaccatt ataaaacaaa aatgtggata
    16561 tttatagttt tgtgaaagta gccatgagtc gatggcttgt ttgctgaaac gaatcgtttt
    16621 tccgatctta atagatggaa gtcctttgcg aatccattca tccaacgtgt tatttgaaat
    16681 tccaaggtag tcacatgctt gttttttgtt taagtatgaa ttgtctatat ttcgatgatg
    16741 aatttgtttt tcaatttctg atgtaattaa acttgaaatt aatatctgaa tttgtttgat
    16801 ttgatcgtct ggtaagataa cttgcatttt aacctctttt ctcgtaaaat ttgcaattgt
    16861 tttttgacat tagttattat gccatagtgt ggactaaaat cagttgattt tttaaaatac
    16921 agaataatat ttcttgtatg tcttccgttt gggaagacaa tttttaaaag aaacgctgtt
    16981 gtatcaacat ttttgatatt ttggaaagtt gtaaaatcat attaaaaacc gttaaataat
    17041 tcttttaaat tcaacaatcg atttaaaatt aattcatata aatcaatttt ataatgatgt
    17101 tttctacgta acttattgtt atcttaattt agaggtggag taatctcgga ttgaacaact
    17161 attggtaata ttta
//
LOCUS       DASFSW010000029        16759 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.029, whole genome shotgun sequence.
ACCESSION   DASFSW010000029 DASFSW010000000
VERSION     DASFSW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16759
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.029"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            200..436
                     /locus_tag="U1099_000756"
     CDS             200..436
                     /locus_tag="U1099_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867091.1"
                     /translation="MNKRIKKKYRPIQALEQWLLAISTTIDIIVNGQNRLYNRVAELE
                     NIIERNAQATNSRFDYLEKKVANKLSKKSWFSRK"
     gene            448..738
                     /locus_tag="U1099_000757"
     CDS             448..738
                     /locus_tag="U1099_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867092.1"
                     /translation="MRYRKKPVEIEAVQWNGRNFEDVYSLCGRSQLNYDEETNTLYIL
                     TLEGVMRADEGCYIIKGIHGEIYPCKEDIFNDTYEAIGPQNIVGFRSVLDSM"
     gene            902..1423
                     /locus_tag="U1099_000758"
     CDS             902..1423
                     /locus_tag="U1099_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEM4867093.1"
                     /translation="MNEETQTVEAVEDDKQVAAEPEQVTTDPKDEKKYTDADVDAIID
                     KEFAKWKAEQEKAESEAKKLAKMNAEDKQKYQLDKREQDLADREAEITRRELTAEAKT
                     ILSERGLPIELVDVVNLADADSVRDSIDAIQKTWEAAVLKGVADKTKGSAPMKKAPQE
                     QPTVEKWERDFLK"
     gene            1441..2457
                     /locus_tag="U1099_000759"
     CDS             1441..2457
                     /locus_tag="U1099_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEM4867094.1"
                     /translation="MPFENINTATSREKFLGIIEKVVAKKSYSAPLLLSNDAVEMNGR
                     SFTVTKSDTTELKDYKRNADNEFDHAQTEERTYTLDQEKYWGRFVDRLDERDSNGEVN
                     VNYVVARQAAEVVAPYLDHLRFDALLGNVSDNVVPANTKGANNSYQAVLDVSEKLDEL
                     DVVENRLLFVTPAFYKAIKSEIVNLPQGDTNQTVLYKGYVGQLDTFTVYKVPSKYLKG
                     VQAVATIGGVVVSPIQVDETKYNNNIPGRFGELVEQLLYTGAFVFDFDQKYIISIATS
                     KPEAKPSAQGKLNIRAEQWVSGATYEAGARVQNEGKLFEATKKVNSSSTAPGSDSANW
                     KEIV"
     gene            2468..2656
                     /locus_tag="U1099_000760"
     CDS             2468..2656
                     /locus_tag="U1099_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867095.1"
                     /translation="MRFKVLKEFTDDELGFVHRVNDIIELTKERHKQMKKNAKLQDVN
                     LADYIEEIKTKGAEAPAK"
     gene            2666..2995
                     /locus_tag="U1099_000761"
     CDS             2666..2995
                     /locus_tag="U1099_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEM4867096.1"
                     /translation="MLEDLKTLTGESDDKILSSLLLRAKNIILTETNRSQLTPALEGL
                     QLEVALELFNRQGSEGETSRSEGGVSVSYKDGLSDTILNGIRSHRLARVAGRAFEAKP
                     TEAVSDS"
     gene            3263..3775
                     /locus_tag="U1099_000762"
     CDS             3263..3775
                     /locus_tag="U1099_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEM4867097.1"
                     /translation="MSRLIGADRLISKLKRLSSQRQTEIIAKAVHNAAKNVVQADAKL
                     RAPVNDGDLRAGIKVRMSKPGNPRAEVVSTSDHGGFVEFGTGPKGATNHAGISPEVSV
                     SYRSTPWYVHESQIDAGPYRFPKIGEFYKMFGQVAQPYLYPALKDNEERVTKNINRYV
                     KRKLIEEVSK"
     gene            3772..4140
                     /locus_tag="U1099_000763"
     CDS             3772..4140
                     /locus_tag="U1099_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867098.1"
                     /translation="MINIKPIIYKKLKEVADNVTDTYPQDWENFPVIIYLEEENKPYE
                     ITDDTEQMSYLRYKVDIFHNDSTSELAVAIDAIFASLGLKRTSSVDTPDPTHLRHKVM
                     RFEGILDLNSRIVYQYRMEG"
     gene            4144..4560
                     /locus_tag="U1099_000764"
     CDS             4144..4560
                     /locus_tag="U1099_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM4867099.1"
                     /translation="MLANGIKLKMSETKGSSYTVIEGLKEVPELGIDPEKVENTTLAD
                     TIKQYEFGIGDAGELEYKFKYENSKTTSSYRTLRKLADAKAVRHFEQEYPDGTKVHFS
                     AQIAVKLGGGGVNSAIEFTLKLALQSDLEFTDPVAL"
     gene            4571..4945
                     /locus_tag="U1099_000765"
     CDS             4571..4945
                     /locus_tag="U1099_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867100.1"
                     /translation="MSTRKPYITWTVKGTDYKLRLSTRQACEVEEKLGVNLLKIFMPQ
                     PGEQFNLPPLKVMLLVVQGALQKFHHGIKLDDVYDLFDDYIDEGYGQTELMSDIIVPL
                     FEVSGFIPRNKEKEEPTLTVVE"
     gene            4972..5331
                     /locus_tag="U1099_000766"
     CDS             4972..5331
                     /locus_tag="U1099_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867101.1"
                     /translation="MINGFYPTALDAGVDPFSFWEYTLLELKELVESYNRQQFQRQKE
                     IASHNFVQSQMIARFVSMMFQEKGEAPDIWDFYPTLFEEDRRQIEQARIERDLIIHRE
                     RMRAFAERMRGRFKTSE"
     gene            5356..8493
                     /locus_tag="U1099_000767"
     CDS             5356..8493
                     /locus_tag="U1099_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867102.1"
                     /translation="MATTLEELRVIVEGEIAPFQKKMKQLESQMKQTQNKIENKTKGL
                     RERVGQQAGGMAAALGKLAKITALAYLGKKMLDLGMYSTQMALEVSASVNQIKRQMGE
                     SSQAFLKWIDNNANAMNMSVGEATKYGAVYSNLFSNFIKDSDKLSAYTGKMLQTSAVI
                     AQGSGRTMTDVMERIRSGLLGNTEAIEDLGINVNVAMIESTNAFKRFANGQSWQQLDY
                     NTQQQIRLMAILEQATAKYGNTLQQSVNGRISLFKSLLSDAALNIGNAMLPIINAMMP
                     VLNSFAMVLKNVTAKLAEFIGLMFNKKANVKNSAIGNLAQGAQNANDAVGGLGDAMDG
                     VDDASGGTADNLDDTAKSAKKAAKELLGLAGFDEITTLNLNKDDSDGAGSGSGGGKGG
                     KNGKGGSSGNGTDILPEIELTDMDNQFKSIFDGWDKTLQPLFDYLSKLKDLFKDGFNM
                     SFRADSLDRFKNALVGIWQSLKDIFADGTVLQAAARFGEKLSYALGQGTGAVANVVMG
                     IAVFIAESLDKSLKETKLDIKNWLIRQFEIKGDTITSIGNLAQAIGQIFYDTITSVAA
                     TDIGSAIISSLIYIRMGIDDITGKIERDFWAFWERLAVDNQAGITTAFIGLLSAVEPI
                     FASIKDLFKNTFISLNATYDEHLKPFFDSFIEGFSSIFGTLIDSWNNDVQPVLDSIGQ
                     LFSDMFDNHIQPFVDNFLYAFGQVVDLLKVVWEEGFLPLFDWIAANILPVLVPIFQTL
                     AEWFVQAWNVVFDVLGAVLKILGGIIEFLVGVFTGDWEKAWDGIVQVAKGMWDLLSSI
                     FMFVWNVILSFLKGVWNTIVAILQAGWDAIVRIFQGLGKWFGDRWKDVENIFSNVGRW
                     FGQKFSEAWNGITNAFSNVVGFFRGIYDNIVSWFSNIGGAVATAVSGAFRYAMNGVFA
                     TIENAVNGFIGMINGVIGLINNIPGVSLGSIGYVNLPRLARGGIVDSPTVAMIGEAGK
                     EVVMPLENTGFLQTMGRVVGGAVVNALGGGLPQSSGLPSGDIVINIGSREFGRFAIDE
                     INKAQAQAGELLLNI"
     gene            8503..8853
                     /locus_tag="U1099_000768"
     CDS             8503..8853
                     /locus_tag="U1099_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867103.1"
                     /translation="MSRLIINGVTVVPPKSFQVAINDVDGETGRNANGDMVRDRITTK
                     RKLECDWGMLTQAEMAQIQNAVQPVFFEVSYPDPILGQTSKTFYVGDRTAPAYSFDEK
                     LKPWSGLKFSLIER"
     gene            8855..14092
                     /locus_tag="U1099_000769"
     CDS             8855..14092
                     /locus_tag="U1099_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867104.1"
                     /translation="MVHTVTFNQAMLAKDRVFAIRAGAYTSSDIKEASFNYGYISGDT
                     LKPGGTVAGSAKLTFTSIITSFNKLDKVYPEIGLKVGDSFEWVAMGEYFVNDINIDRN
                     RNTTELDLMDGMFKLNQPYISDLTYPAQIRDVIREICVKTGVELETDDLGFRAIQHHI
                     QSKADKKDITFREVLSQAIQLLGFSAFFNRKGKLEIRGLTESNITITADNYFLHGLTK
                     SELMYQIAGITCKKDKETLTVGLRTGRSLELENSFMIQNILDDLYYDLKEIKYYPFSL
                     DWQGHLKLDVGQWITLKTNKNETFKVPVLSQSFNFKGGLKSKISADSKAGNDTQYSYK
                     GFLGKRIEQMSTEIEAEVQQQLEYKDKEFDEKINKAKSEINDGIEQAQAEAERYADAI
                     KQEIDTEIAQVNQSMQSQEQEHDREVANILSKTQSVESLANQAKADAANAIARANQVK
                     TEAIADARAQVATVNQALNTAKTDLQNQVNAIDAKAVQAQRDITQAKYDLQSQASQLI
                     AQATKQVELTKLTTETKKLADGTLTSLNELTKTVDKTTGDLTSVTNRTKVVEDSLAGV
                     KTNYTQLNQTVNAQTGQIDSINRKTADLQSGIDGVTERFKSLKIGSRNYFKNSKSRKY
                     YITSLTTQDVRTYIDEEFWQNDTRFVKDYVRVSFDIAFNPALQSDFTTTVHFSATPWY
                     GAGITFKGGTTALQHFDLKFNLSDATKAYKTDNVFIRLTNTIPLNTAVSLENFNLYLS
                     AVIEDYSQSNADFESKIAEYKRTADQNYAGLQSTVSTLDGKVTQNKTEANQTATQLSN
                     RLTSLETYKDGESTRAQSYFEASKTETAKQLTAERTAIATNYVAKSTYTEDVRGTTQK
                     LNEIKSTADTAKQNLATYQNTVDRKLEELTSSTQTLDGKINTASAKVDTVAGQIRTEI
                     GTVEAKIPTEAGGRNYILKSQAEISSTGRWVSKPFNLSSDLLSNLSKIKTVTISCDVE
                     GINVSALNSRKRYGLACSVEINGVVNYWEVWQTQDTTKKRISQTFTVPEGKVITKFHS
                     PTLWIQAAGDIKVSNPKIEFGRVPTDHTLAPEDFANELSSVKTTITQTASGVEQLSTS
                     LATTDNKVTTAEAKIRQLISDVSSKVSQTDYNTLTGRVDDAETAITQNATEISKRLTK
                     TQVDKAITDKGFQTASQVDTAIAGKGYQTKSDVDNNITGRGYITNSALQPYALSTTVQ
                     NLVQETAGSFERQITETRGLIPTEVNQNLFDVVFSDLRATGGASFVQSDDGWIEVTIK
                     NRWSGFHWYYSDDINPSKKQYVVSYEAYLVDTVAETARLESDFGSPDQLTLINKTPKR
                     YTVVLNRPANIYNYINFVCNSSETGKKFRIRNIKLEEGIVATPYIAPFPTTVLFNAVK
                     DTVDAHKRLIGNGDSISQAIQSANKFERSISSGGDVYQAIETAKGLVTEVSGTNGLKI
                     QVSQLAGSWAVQNLTSSGQILNQINLLANGTNRIDGRLTHITGQTLIDNGVIINAMIG
                     NLDAGKIKTGTLDAARIRANSIDGSKIVFDQAFLNKMTANEALFKQLFAQSAFITSVQ
                     AVAVSAKQIAGGIAKALNGGMDVNFDESKINFYTNVAAIRRIYTGHPTQFIKFETEGN
                     YSRTIIGSNRNGGEVFNSATFAGIVVENTNNINTEDNVRIYGDNTLLRHAQGDVGWNI
                     NSVTQRIVPANMNAESEIWSKHFVAPDKNSKPVRLDTAVAALWDIWNHIIYNNFEFNA
                     ALRTHIKARRDNWKFELNL"
     gene            14101..14319
                     /locus_tag="U1099_000770"
     CDS             14101..14319
                     /locus_tag="U1099_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867105.1"
                     /translation="MNQEQITQALRLTNNDLVTKLSEEMTTKNLLAVQLTEAQQTIAN
                     LRAEITDLTQQLDEATKPEEIIDQEEGE"
     gene            14322..14666
                     /locus_tag="U1099_000771"
     CDS             14322..14666
                     /locus_tag="U1099_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867106.1"
                     /translation="MTETTNNTLLNLEETTQPFDLATALQYMKENGEFIRCKNATNDF
                     YMYRDVQKRPAIVNGRRKFVDVETIWAFNQWGGTAATINIADMLNEEYWIMKFDENGN
                     PDWTDPTAGAEA"
     gene            14670..14969
                     /locus_tag="U1099_000772"
     CDS             14670..14969
                     /locus_tag="U1099_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867107.1"
                     /translation="MPIEHAERIAQSQVAWAILFILLFGFVIRYLIKTSDKREAKLMD
                     FHEQAKEESNKREDRLMNHLEKTTAEMGAMAREIGGLKGEVSLMSDRIEKIEKGE"
     gene            14972..15301
                     /locus_tag="U1099_000773"
     CDS             14972..15301
                     /locus_tag="U1099_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM4867108.1"
                     /translation="MNQLTEIIIGSATGILAIVAGMIVHEVKKYLIAKGGKRAVEITE
                     ILARNAVNAVEQIAKLDQDKHVDKLDMAKRRVTGQLAKYNIYMTDTQLETFIESAVKQ
                     MNDAWKE"
     gene            15304..16710
                     /locus_tag="U1099_000774"
     CDS             15304..16710
                     /locus_tag="U1099_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM4867109.1"
                     /translation="MTINLETSIRWMSDRVGKVIYSMDYRNGPNSYDCSSAVYYALMA
                     GGAISAGWAVNTEYMHDWLIRNGYVLVAENKPFNAQRHDVFIWGKHGYSSGEGGHTGI
                     FVDNVNIIHCNFKRNGITIDDYNNVSRGMYYYLYRPANQPSTSNKSLDQLVKETLAGI
                     HGNGDARKASLGNQYEPVMAVINGKATAPKKTIDELVQEVIAGKHGNGEERKKSLGPD
                     YDVVQKRVSELLKKQSSEPLNGQEVKQVAETKISQTEPTGQATAGKEEGDLSFNGAIL
                     KKSVLDVILAKCKEHNILPSYAITVLHFEGLWGTSAVGKADNNWGGMTMTSNDLQITR
                     PSGVIVTRGLARPSNEGGYYMHYASVDDFLTDWFYLLRAGGSYKVSGAKTFSEAVKGM
                     FKVGGAVYDYAATGYENYLVGMSSRLKAIEAENGSLAKYDTATVTDVGSTDKIEVNIE
                     GIEISINGVTYTLSKKPV"
BASE COUNT         5330 a         3129 c         3915 g         4385 t
ORIGIN      
        1 agtcgagtgc ttttttgttg cagaaaatag gagggagtat gtaccaacta agtaaaataa
       61 aaaaatggtt catcaagact ttcttatgtg ttcatgattt ttggttcaaa gatttagggc
      121 attataagat tgatttttat tattgcaaga agtgtggtaa ggtcactaaa agattataat
      181 ttttggaaag gagaacgcca tgaataagcg gattaaaaag aaatatcgtc caatacaagc
      241 tttggaacaa tggctactgg ctatttctac aacgatagac attatcgtca acggtcaaaa
      301 ccggctatat aatcgtgtgg ctgagctaga gaatattatc gaacgcaacg cccaggctac
      361 gaattcgaga tttgattatc ttgaaaagaa agtagctaac aagctgtcca agaagtcttg
      421 gtttagtcgt aaataaagga gtaaaaaatg cgttatcgta agaaacctgt tgaaattgaa
      481 gcagttcagt ggaacggtag aaattttgaa gatgtttaca gtctttgtgg tagaagtcaa
      541 cttaactatg acgaagaaac aaacacgttg tacattttga ctttggaagg tgtaatgcga
      601 gctgacgaag gctgttatat tattaaaggt atccacggag aaatatatcc atgtaaggaa
      661 gatattttca atgatactta tgaggcgatt ggaccccaaa atattgttgg ttttcgcagt
      721 gttttggatt ctatgtgagg aggtggtcac tcatcttgac agcaggaaag actgcttgaa
      781 actactctaa attactttaa actggtcgaa attgaccagt tttctttttg gtccaagcat
      841 tgaagacggt aaaagctatg gaaaaacagt cggggacgac tttaaaaata ggaggttcgc
      901 aatgaacgaa gaaacacaaa cagtcgaagc ggtcgaagat gacaaacagg tagcagctga
      961 acctgaacaa gtcacaacag acccgaaaga cgaaaagaag tacaccgatg ccgatgttga
     1021 tgctatcatt gacaaggaat ttgctaaatg gaaagcagag caggaaaaag ctgaatcaga
     1081 agctaagaaa ttagccaaga tgaacgctga agacaagcag aagtaccagc ttgataagcg
     1141 tgaacaggac cttgctgacc gtgaagcaga aatcacacgc cgagagctaa ccgctgaagc
     1201 taagacgatt ttaagcgaac gtggcttacc aatcgagtta gtagacgtgg ttaatcttgc
     1261 tgacgctgat agtgtacgtg attccattga tgctattcaa aagacttggg aagcagcagt
     1321 cttaaaaggt gttgctgata agaccaaagg aagtgcaccg atgaagaaag cgccacaaga
     1381 acaaccaacc gttgaaaagt gggaacgtga ttttttgaaa taagaaagga aaaataaaat
     1441 atgccatttg aaaatattaa caccgcaaca tcccgtgaaa aattcttagg gattatcgag
     1501 aaagttgttg ctaaaaaatc ttactcagct ccgcttttgc tatcgaatga tgcagtggaa
     1561 atgaacgggc gttcatttac tgttacaaaa tctgacacaa cagaactcaa agactacaag
     1621 cgtaatgctg acaatgagtt cgaccatgca caaacagaag aacgcacata cactctggac
     1681 caagaaaaat actgggggcg ttttgtggat cgtctagatg agcgtgattc aaacggtgaa
     1741 gtcaatgtca attatgttgt cgctcgtcaa gctgccgaag ttgtggcgcc gtacttagac
     1801 catcttcgtt ttgacgctct tcttggtaat gtcagcgata atgtcgtacc agctaatact
     1861 aaaggtgcta ataattctta ccaagctgtc ttggatgttt ctgagaaatt ggatgagttg
     1921 gatgtggttg aaaaccgctt gttgtttgtg acgccagcat tttataaagc gattaagtca
     1981 gaaattgtca accttccaca aggggacacc aatcaaactg tgctttacaa aggttatgtc
     2041 ggtcagttgg atacattcac ggtttacaaa gtaccatcta agtaccttaa aggtgtacaa
     2101 gctgttgcga caatcggtgg ggtagtcgtt tcgcctatcc aagtagatga aacgaaatat
     2161 aacaacaaca tcccaggtcg ttttggtgaa ttggtggaac aattgctgta tactggtgca
     2221 tttgtctttg attttgacca aaagtacatc atttctattg cgacctctaa accagaagcg
     2281 aagccaagcg cacaaggtaa attgaacatc cgtgctgagc aatgggtttc tggtgcaact
     2341 tatgaagctg gcgcccgtgt acaaaacgaa ggtaagttgt ttgaagccac taagaaagta
     2401 aattcatcct caacagctcc tggtagcgat tcagccaact ggaaagagat tgtctaggag
     2461 gccctaaatg cgctttaagg tattaaaaga gtttactgat gacgaacttg gttttgttca
     2521 tcgtgttaac gatatcatcg aattgaccaa ggaacgtcat aagcagatga agaaaaacgc
     2581 taaattgcaa gacgtgaatt tggctgatta cattgaagaa atcaagacca aaggagcaga
     2641 agctcctgca aaataggggg cggatatgct agaagattta aaaactttaa caggcgagag
     2701 tgatgataaa atcctctcgt ctttgctttt gagggcaaaa aatatcattt tgactgagac
     2761 aaatcgaagt cagcttacgc cagcgctgga aggtttgcaa ctggaagtgg cactcgagtt
     2821 gttcaatcgc caaggaagcg agggcgaaac atcacggagt gaagggggcg tgtctgtgtc
     2881 ttataaagac gggctgtcag atactatttt gaatggtatt cggagtcata gactcgcaag
     2941 ggtggcaggt cgtgcgtttg aagcgaaacc gactgaagcc gtatctgatt cgtaaagctg
     3001 tcatagtgac gagtgatgag ggtatcaaga aagctactta cagcgatgtt tctactgaga
     3061 tacgggccga gatatggcct gctagtggtc gtttacaagc tgagatatac ggtcagagat
     3121 tggcatatat tttgaattgc ttggtagacc gtgagactct tatagatgaa ggcgatggct
     3181 tttgtatcaa cagcgataaa gtaacccata aagtcatatc cataaagcga tatacaaatc
     3241 atcaagtctt ggagttggaa caatgtcgcg attgataggt gctgataggt taatctcgaa
     3301 gctcaaacga ttgtctagtc aacgacagac cgaaatcata gcgaaagctg tacacaacgc
     3361 tgctaagaat gttgtacaag ctgatgcaaa gttgcgagct cctgtcaacg atggtgattt
     3421 gagagcgggt atcaaggtcc gcatgtccaa gcctgggaat ccaagagctg aagtcgtaag
     3481 tacgtcggac catggtggat ttgtcgaatt tggaacaggt ccaaaaggcg caacaaacca
     3541 tgcaggcatt tcgccagaag tcagcgtgtc ctatcgaagc acaccttggt atgtccacga
     3601 gtctcagatt gatgccggtc cttatcgttt ccccaagatt ggcgagtttt acaagatgtt
     3661 tggtcaagtt gcccaaccgt atctttatcc agcacttaag gataatgagg aacgggtcac
     3721 taagaacatc aacagatatg ttaagcgaaa attgattgaa gaggtcagca aatgataaat
     3781 attaagccca tcatttacaa gaaattgaaa gaggttgcgg acaatgtgac agatacttat
     3841 ccgcaggatt gggagaattt cccggttatc atctacttag aagaggaaaa caagccttac
     3901 gagattacag atgatacaga acagatgtcc tatttgcgct acaaggtcga tattttccac
     3961 aatgatagta cgtcagaatt agcagtagcg attgatgcga tttttgcatc tctcgggcta
     4021 aaacgtacat ccagcgtgga tacacccgac ccaacgcact tacgacacaa agtcatgcga
     4081 tttgagggga ttttagatct aaactcccga atcgtttacc aatacagaat ggaaggataa
     4141 aacatgttag cgaatggaat taaattgaaa atgagcgaga ccaaagggtc tagctataca
     4201 gttatcgagg gcttgaaaga agttccagaa cttggtattg accctgagaa agttgagaat
     4261 acgacccttg cggataccat taagcagtat gaatttggta ttggtgacgc tggtgaattg
     4321 gaatataaat tcaagtatga gaattccaaa acaacttcta gttatcgtac tttgcgtaaa
     4381 ctggcagatg ccaaggctgt tcgtcacttt gaacaagaat acccagatgg tacgaaggtg
     4441 catttctcgg ctcaaatcgc agtcaaattg ggcggtggag gcgtgaattc tgcaatcgaa
     4501 tttacattga aattggctct acagtcagat ttagaattca ctgatccagt agcactttaa
     4561 ggaggtataa atgtcaacac gtaaaccata tatcacttgg accgtcaagg gaacagacta
     4621 taaattgcgt cttagcactc gccaagcctg tgaagttgaa gaaaaattgg gcgttaattt
     4681 gctcaaaatc tttatgccac aaccaggcga acagttcaat ctaccgcctt tgaaggtcat
     4741 gttgttggtt gttcaaggtg ctttgcagaa gttccatcat ggtatcaaat tggatgatgt
     4801 ctatgacttg ttcgatgatt acattgatga aggctacgga caaactgaat tgatgtccga
     4861 tatcatcgta ccattgtttg aagtatcggg ttttattcct cggaacaagg agaaggaaga
     4921 accgacgttg acagtagtcg agtaggttct ggtccttgtt cggtcgcaga attgattaac
     4981 gggttttatc caacagcatt agatgcaggg gtagacccgt tttctttttg ggaatacact
     5041 cttttggaat tgaaagagtt ggtcgagagt tacaacaggc aacaattcca gagacagaaa
     5101 gaaattgctt cgcataactt tgttcaatcg caaatgatag ctcgttttgt ttccatgatg
     5161 ttccaggaaa aaggtgaagc accggacatt tgggacttct accctacttt gttcgaagag
     5221 gataggcgac agatagaaca agctcgaatt gagagagatt tgataattca tcgcgaacgg
     5281 atgagggctt ttgctgaaag aatgagagga agattcaaaa cttccgaata agataacatg
     5341 gaaaggaggg gaactatggc tacaacctta gaagagttga gagttattgt cgaaggcgag
     5401 attgctccat ttcaaaaaaa gatgaagcaa ttggaatctc agatgaagca aactcaaaac
     5461 aaaattgaaa acaagacaaa aggtcttaga gagcgtgtag gtcaacaagc tggtggtatg
     5521 gcggctgctt tgggcaaact tgctaagatt actgcgttag cttacctagg caagaaaatg
     5581 ttagacctcg gcatgtattc tacgcagatg gctcttgaag tgagtgcttc agtcaatcaa
     5641 atcaaacggc agatgggcga aagttcccaa gcgtttttaa agtggattga taacaatgcc
     5701 aacgcaatga atatgagtgt tggagaagct accaagtacg gagctgtcta ttccaatcta
     5761 ttttccaact ttatcaaaga ttccgacaaa ctgagcgctt atacaggcaa gatgttacag
     5821 acatctgctg ttattgcgca aggtagcgga cggaccatga cagatgttat ggaacgtatc
     5881 cgatcaggct tgctagggaa caccgaagcg attgaagacc ttggaatcaa cgtcaatgtc
     5941 gccatgattg agtccacaaa tgcctttaag cgatttgcga acgggcaatc ttggcaacag
     6001 ttagactaca atacccagca acaaattcgc ttgatggcta ttttggagca agcgacagcc
     6061 aagtacggca ataccttgca acagtctgta aacgggcgta ttagcttgtt caaatcgctt
     6121 ttgagcgatg cggcgctaaa tattggtaat gctatgttac cgattattaa tgccatgatg
     6181 cctgtgctaa attcttttgc gatggtactc aaaaatgtca ctgctaaact tgctgagttt
     6241 atcggtttga tgtttaacaa aaaagccaac gtgaagaata gcgcaatcgg aaatcttgct
     6301 cagggtgcac aaaacgcaaa tgacgcagta ggcggtctag gcgatgccat ggacggtgta
     6361 gatgacgcgt ccggtgggac tgcagacaat ctagatgata ctgccaaatc agctaagaaa
     6421 gctgctaaag aacttctggg cttagcagga ttcgatgaaa tcactaccct aaatttgaac
     6481 aaagacgact cagacggagc tggtagcggt tccggcggag ggaagggcgg caaaaatggc
     6541 aagggaggga gttctgggaa cggaactgac atcttacctg aaatagaatt gactgatatg
     6601 gacaaccagt ttaagtccat atttgacgga tgggataaaa ccctacaacc tctttttgat
     6661 tacctctcaa aattaaaaga cctgtttaaa gacggcttta atatgtcgtt tagagctgac
     6721 agcttggacc gttttaaaaa cgctttggtt ggtatctggc aatctctaaa agacatcttt
     6781 gcagacggaa ctgtcttgca agcagcagca aggtttggcg agaaattatc ctacgctctg
     6841 ggacaaggaa cgggagctgt cgcaaatgtc gtgatgggca ttgcagtctt tattgccgaa
     6901 agtctagata aatccttaaa agaaactaag ctcgatataa agaattggct aattagacaa
     6961 tttgagataa aaggcgacac tatcactagt atcggaaatt tagctcaggc tattggtcaa
     7021 attttttacg atacaatcac aagcgtagca gcgaccgata tcggttctgc cattatctcc
     7081 tcactaatct atatccggat ggggatagat gatataaccg gaaagattga acgagatttt
     7141 tgggcgtttt gggagcgtct agccgtagat aatcaagcag gtattacaac ggcatttatc
     7201 ggcttattat cagctgtaga acctatcttc gcgtctatca aggacttgtt caagaatacc
     7261 tttatcagct taaatgcaac ctatgatgaa cacttaaagc cgttctttga ttcgttcatt
     7321 gagggcttca gttctatctt tggcactctg atagacagtt ggaacaatga tgttcaacca
     7381 gtattagata gcatcggaca attgttctct gatatgtttg ataaccatat tcagcctttt
     7441 gttgataatt tcctatacgc attcggtcaa gtagtagatt tattgaaagt tgtatgggaa
     7501 gaggggtttt tgccactctt tgattggatt gcagcgaaca tactaccagt acttgttcca
     7561 atattccaaa cacttgcaga atggttcgtg caagcgtgga atgttgtttt cgatgtttta
     7621 ggagctgttt taaaaatcct aggcggtatc atcgagtttc tggtcggtgt atttacaggc
     7681 gactgggaaa aggcttggga cggcattgtt caagtagcaa aaggaatgtg ggatttactg
     7741 tcgtctattt tcatgttcgt ttggaacgtc attctatcat tcttaaaagg tgtctggaat
     7801 accattgttg caatattaca ggctggatgg gatgctattg tccgcatctt ccaaggttta
     7861 ggtaaatggt ttggtgaccg ctggaaagat gttgagaata tattttccaa cgtaggtaga
     7921 tggtttggac agaagttttc tgaggcatgg aatggtatta caaatgcttt cagcaatgtt
     7981 gtaggatttt tccgtggcat ttatgataac atcgtcagtt ggtttagcaa cattggtggc
     8041 gctgtagcaa ctgctgtttc tggtgcattt cgttatgcga tgaatggtgt gtttgccact
     8101 attgagaacg ctgtaaatgg ctttatcggt atgattaacg gtgttattgg tttaatcaat
     8161 aacattccag gcgttagcct aggtagcatt ggctacgtca accttcctcg tcttgcccgt
     8221 ggtggtattg tggacagccc taccgttgcc atgattgggg aggctggtaa agaggtagtt
     8281 atgccgttgg aaaatacagg cttcctgcaa acaatgggac gagttgtcgg cggtgcagtt
     8341 gttaatgctt tgggcggagg tctaccgcaa tcgtctggat tgccaagtgg tgacatcgtc
     8401 atcaatatcg gcagccgaga attcggacgc tttgcgattg atgagattaa caaagcacag
     8461 gcgcaagctg gcgaactgtt attaaacatt taggagggaa acatgagtcg attgattatc
     8521 aatggagtta cagtagtacc tcctaaatct tttcaagtcg ctatcaatga tgtagatggc
     8581 gagacaggtc gaaatgctaa cggagacatg gtcagggata ggattacaac caagcgcaaa
     8641 ttggaatgtg attgggggat gttgacacag gctgagatgg ctcagataca gaatgctgtt
     8701 cagcctgttt tctttgaagt atcctatccg gaccctatct tagggcagac ctctaaaaca
     8761 ttctatgttg gtgacagaac agcaccagca tattcatttg atgaaaaact caaaccatgg
     8821 agcggtttaa aatttagttt aatagagagg taaggtggtt cacacggtaa catttaacca
     8881 agctatgtta gctaaagata gggtgtttgc tattcgtgca ggcgcctata cttctagcga
     8941 catcaaagaa gctagtttca attatggata tatcagcggc gatactttaa aacctggcgg
     9001 aacagttgct ggttcggcta aattgacctt tacatctatc atcactagct ttaacaaatt
     9061 ggataaagtt tatccagaga taggactaaa agttggcgat tccttcgagt gggttgcaat
     9121 gggtgagtat tttgtcaacg atattaacat cgaccgcaac aggaatacca cagaattaga
     9181 tctgatggat gggatgttca agctcaatca accttatatt tctgacctga cttacccggc
     9241 acagattaga gatgtcattc gcgaaatttg tgtaaagaca ggagtagagt tagaaacaga
     9301 tgatttaggt ttccgagcga ttcagcatca tatccaatca aaagcggata aaaaggacat
     9361 tacttttaga gaagtactaa gtcaagcgat tcaattgctt ggcttttctg ctttttttaa
     9421 cagaaaaggc aaattggaaa ttcgtgggtt gactgaatca aatatcacaa ttactgctga
     9481 taattatttt ttgcacggcc tgactaaaag cgaacttatg taccagattg caggtatcac
     9541 ttgcaagaaa gacaaagaga cgttaacagt tggattgcga actggtcgct ctttagagct
     9601 agaaaatagc ttcatgatac agaatatctt agacgatttg tattatgatt tgaaagaaat
     9661 caagtattat ccattttctc ttgattggca aggacaccta aaactggatg tcgggcaatg
     9721 gattacgtta aaaacaaaca aaaacgagac ttttaaagtc cctgtactga gtcaatcttt
     9781 taatttcaag ggcggtctaa aatccaaaat tagtgcagac agcaaagctg gtaatgatac
     9841 tcagtattct tataagggat ttttaggcaa gcgcatcgag caaatgtcta ctgagatcga
     9901 agcagaggtt caacagcaac tggaatataa ggataaggaa ttcgatgaaa aaattaacaa
     9961 agccaaatcc gaaatcaatg acggtatcga gcaagcccaa gctgaggctg agcggtatgc
    10021 tgacgctatt aaacaggaaa ttgatactga aatcgcccaa gtcaaccaat ccatgcaatc
    10081 ccaggaacag gaacacgaca gagaggttgc gaacatcctg tctaaaaccc agtctgtcga
    10141 gtcgcttgcc aaccaggcca aggcagatgc ggcaaacgcc atcgctagag ctaaccaggt
    10201 caagaccgaa gctatcgcag atgcaagagc gcaggttgcg accgttaatc aagcgttaaa
    10261 tactgctaag actgatttgc aaaatcaagt taacgcgatt gatgctaagg ctgtgcaagc
    10321 acaacgagat atcacacaag ctaagtatga tttacaaagt caggcctcac agcttatcgc
    10381 acaagcgact aaacaagttg agttgaccaa gcttaccacc gaaacgaaaa agcttgcaga
    10441 tggcacgcta acgagcttaa acgagctgac taaaactgtc gacaaaacga ctggcgacct
    10501 tacgagcgtg accaatcgta ctaaagtcgt tgaggatagt ctagctggtg ttaaaactaa
    10561 ttacactcaa ctcaatcaga cggtaaatgc ccagactggt caaattgata gtatcaatcg
    10621 taagactgct gatttacaaa gtggtattga tggcgttaca gagcggttta agagtttaaa
    10681 aattggtagt cgtaactatt ttaaaaactc aaaatctcgt aaatactaca ttactagttt
    10741 aacaactcaa gatgtgagga cttatattga tgaggagttt tggcaaaatg atacacgatt
    10801 tgtcaaagat tatgtaaggg tgtcttttga tatagcgttt aatccagcct tacaatcaga
    10861 ctttacaaca actgtccatt tttcggcaac tccgtggtat ggagcaggta tcacgtttaa
    10921 aggtggcaca actgcgttac aacattttga cttaaagttt aatttgagcg acgcaacaaa
    10981 agcttataag acagacaatg tattcatccg tctcacaaat acaatccccc tcaataccgc
    11041 tgtaagtctc gaaaatttta atctatattt atccgcagta atcgaggatt actctcaaag
    11101 caacgctgat tttgagtcca aaatcgccga gtacaagcgg actgccgacc aaaactacgc
    11161 tggcttacaa tcaaccgtta gcacgttaga tggtaaggtc actcagaata agaccgaagc
    11221 caatcagaca gctacgcagt tatctaacag attaacaagc cttgaaacat acaaggacgg
    11281 cgaatcaact cgcgctcagt cgtattttga ggcatcaaag acagagacgg ccaaacagtt
    11341 aacagccgag cgtactgcaa ttgctactaa ttatgtggct aagtcaacat acacagagga
    11401 tgtccgtgga acgacccaaa aacttaacga gattaagtca accgctgaca ctgcaaagca
    11461 aaatttagca acgtatcaaa acacggttga ccgtaagttg gaagaactga catcaagtac
    11521 tcaaacgctt gacggtaaaa tcaatacagc gagcgcaaag gttgatactg tagccggtca
    11581 gatacggact gagattggca ctgttgaggc gaagattccg actgaggcag gcggaagaaa
    11641 ttacattttg aaatctcaag ccgaaatcag tagcacaggt aggtgggtaa gcaaaccatt
    11701 taatttgtcg agtgacttac tatctaattt gtcgaaaatc aaaactgtca caatatcttg
    11761 tgatgtcgag ggtatcaatg tttccgcttt aaattcacga aaaaggtatg gtttagcttg
    11821 ttcagttgag ataaacggtg tagtgaatta ttgggaagtt tggcaaacgc aagataccac
    11881 gaagaagcga attagccaga cgtttactgt tcctgaaggg aaagtaatta ccaaatttca
    11941 ctcgccgaca ttatggatac aggcagccgg agatataaaa gtttccaatc ccaaaatcga
    12001 gtttggaagg gtacctacag atcacacatt ggcgcctgaa gattttgcca atgaattatc
    12061 atcagtcaaa accacaatca ctcagactgc atccggtgta gagcagttat caactagctt
    12121 agctacgact gataacaaag tcacgactgc tgaggctaaa atccgacagt taattagcga
    12181 tgtgtcaagc aaagtatcgc aaacggacta caatacgctg accggccgtg tggatgatgc
    12241 tgaaacagct attactcaaa atgcgaccga gattagcaag cgattgacaa agacgcaagt
    12301 tgataaagca atcacagata aagggtttca gactgcatcg caagtagaca ctgcaatcgc
    12361 tggtaaaggt taccagacca agtctgatgt tgacaacaac atcacaggtc gtgggtatat
    12421 cactaatagt gcattgcaac cttatgcatt atcaacgact gtgcagaatc ttgtgcaaga
    12481 gacagctggt agctttgaac gtcagattac agaaacaaga ggtctgatac cgacggaagt
    12541 caatcaaaat ctttttgatg ttgtttttag tgatttaaga gcaactggag gtgctagctt
    12601 tgtacaatct gatgatggtt ggattgaggt tacgattaaa aataggtggt ctggattcca
    12661 ttggtattac tcagatgata tcaacccgtc aaaaaaacaa tatgtggtca gttatgaagc
    12721 ttatttggtt gatactgttg cagaaacagc acgacttgaa tcagactttg gcagtccaga
    12781 ccagttgaca ctcatcaaca aaacaccgaa gcgttataca gttgtattaa atagacctgc
    12841 taatatctat aattacatca attttgtgtg taattcctca gaaactggta aaaaatttag
    12901 aattcgaaat attaagcttg aggaagggat tgttgcgact ccttacattg cgccattccc
    12961 aacaacagta ttgtttaatg ctgttaaaga caccgtagac gctcataagc ggcttattgg
    13021 caatggcgat agcatatcgc aagcaatcca atctgcaaac aaatttgagc ggtctatttc
    13081 aagcggtggt gacgtttatc aagcgattga gacggctaaa ggactagtca cagaggtgtc
    13141 tggtactaac ggactcaaga tacaagtcag tcaattagct gggtcttggg cagtccaaaa
    13201 cttgactagt agcggtcaga tattaaacca aattaactta cttgccaatg gcacaaacag
    13261 gattgacggt cgattgacgc acatcacagg tcaaacttta attgataatg gcgtcatcat
    13321 caacgcaatg attggcaatt tagatgcagg gaaaatcaag acaggcacgc ttgatgcagc
    13381 acgcattcgg gctaactcga ttgatggcag taagatagtc tttgaccagg cgtttttaaa
    13441 taagatgacc gctaacgaag ctttgtttaa gcagctgttt gctcaaagcg catttatcac
    13501 aagcgtccag gcagtggctg tgtcagctaa acagattgct ggcggtattg ctaaagcact
    13561 caacggtggt atggatgtca atttcgacga aagtaaaatc aacttttaca caaacgtagc
    13621 tgcaataaga cgtatctata ctggacaccc tactcaattt ataaaattcg aaaccgaagg
    13681 gaattactcg cgaacaatca tcgggagtaa ccgtaatgga ggagaagttt ttaattcggc
    13741 aacatttgca gggattgttg tagagaacac aaacaatata aacacagaag acaatgtgag
    13801 gatttatgga gataacacgc tattaagaca tgcacaaggc gatgtcggtt ggaatatcaa
    13861 ttctgtcact caacgtatag tcccagctaa catgaacgca gagtccgaaa tttggtctaa
    13921 gcactttgtg gctccggata aaaattcaaa gcctgtccga ttggatacag cagtggcagc
    13981 attatgggac atttggaatc acatcatcta caacaatttt gagttcaacg cagcgcttcg
    14041 cacacacata aaagctagac gggacaactg gaaatttgaa ttaaatttat aggaggaatc
    14101 atgaatcaag aacaaatcac tcaagcgcta cgcttgacta ataacgacct cgtgacaaaa
    14161 ctgtcagagg agatgacgac gaaaaacttg ctcgctgtgc aactaactga ggcacagcag
    14221 accatcgcta atctgcgggc agaaattact gacttaactc agcaactgga tgaagctact
    14281 aaaccagagg aaatcattga ccaagaagaa ggagaataat catgacagaa actactaaca
    14341 acacattgct taatttggaa gaaacaacac aaccatttga ccttgcgact gcattgcagt
    14401 atatgaagga aaacggggaa ttcattcgct gcaagaatgc gacaaatgat ttttatatgt
    14461 accgtgatgt gcaaaaacgt cctgcaattg tgaatggtcg tcgcaaattt gtggatgtgg
    14521 aaactatctg ggcctttaat cagtggggcg gtaccgctgc gacaattaat attgctgaca
    14581 tgctcaacga agagtattgg atcatgaaat ttgatgaaaa cggaaatcca gattggacag
    14641 acccaacagc aggagcggag gcgtagccta tgccaatcga acacgcagaa agaatagccc
    14701 aaagtcaagt tgcttgggct attttgttta ttttattatt cggattcgtc atccgttatc
    14761 tgattaagac gtcagacaag cgagaagcta agctcatgga tttccatgag caggccaagg
    14821 aagaaagtaa caagcgggag gaccgcttga tgaatcatct tgaaaagact accgcagaaa
    14881 tgggagccat ggctcgtgaa atcggtggct taaaaggtga agtgtcatta atgagtgacc
    14941 gtattgaaaa aatcgaaaaa ggagaataag tatgaatcaa cttacagaaa ttattatagg
    15001 gtctgctact ggtatattag ctatcgttgc tggtatgatt gtccatgagg tcaaaaagta
    15061 tctgattgcc aagggtggta agcgagcggt cgaaattaca gagattctgg cacggaacgc
    15121 tgttaatgca gttgaacaaa tcgccaagct agaccaggac aagcatgtag acaagctaga
    15181 catggctaaa cgtcgcgtaa caggtcagct tgctaaatac aacatctata tgactgatac
    15241 acagttagag acctttatcg aatcagcagt gaagcaaatg aacgatgcct ggaaggagta
    15301 actatgacaa tcaatcttga aacatccatt cgttggatga gcgaccgtgt cggcaaagtc
    15361 atttactcaa tggactatcg gaacggtccg aatagttatg actgttctag tgctgtatat
    15421 tatgcgctaa tggcgggtgg tgcaatttct gcaggttggg cggttaacac tgagtatatg
    15481 catgactggt tgatacgtaa cggatatgtt ttggttgctg aaaataaacc atttaatgct
    15541 caaagacatg atgtttttat ttggggtaaa catggttatt ccagcggtga aggtgggcac
    15601 actgggatat ttgtggataa tgtcaacatt atccattgta attttaaacg caatggcatt
    15661 actattgatg attacaataa tgtatctcgt ggtatgtatt actatctata tcgtccggca
    15721 aatcagccca gcaccagcaa caaatcattg gatcagcttg ttaaggagac tttggctggg
    15781 atacatggta acggagatgc ccgcaaagca agtttgggca atcaatatga acctgtcatg
    15841 gcagttatta atggcaaagc tacggcacct aaaaagacta ttgacgagct cgttcaggag
    15901 gtaattgccg gcaaacacgg caacggagaa gaacgtaaaa aatccctcgg gcccgattat
    15961 gatgtcgtgc aaaaacgtgt gtctgaattg ctcaaaaaac agtcctcaga gccgttaaat
    16021 ggtcaagagg taaaacaggt cgcggaaacc aaaataagcc aaactgagcc aactggacaa
    16081 gccacagcag gcaaagaaga gggagaccta tctttcaatg gtgcaattct caaaaaatct
    16141 gtcctagatg ttatccttgc caagtgcaaa gagcacaata tcctacctag ctacgctatt
    16201 accgtcctac actttgaggg gctttggggt acctcagccg taggtaaggc agataacaac
    16261 tggggcggta tgactatgac aagcaatgac ttgcaaatca ctcgtccctc aggagttatt
    16321 gtcactagag gtcttgctcg tccgtcaaac gaaggcggat actatatgca ctatgctagt
    16381 gtggatgatt tcttgacaga ctggttctac ttgctaaggg ctggtggctc ttacaaggtt
    16441 tcaggagcta aaacctttag cgaggcagtc aagggcatgt tcaaagttgg tggcgcagtc
    16501 tatgattatg ctgctacagg ctatgagaat tacctggtag ggatgtcaag ccgtctgaaa
    16561 gctattgagg cagaaaacgg atcactagcc aagtatgata ctgctaccgt cacagatgtc
    16621 ggtagcacag acaagattga ggtcaacatt gaggggattg aaatttctat caatggagtg
    16681 acctacacac tttcaaaaaa accagtttaa gataagatac aaaaagccct taggacaaaa
    16741 tcctaggggc tttttttcg
//
LOCUS       DASFSW010000030        16475 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.030, whole genome shotgun sequence.
ACCESSION   DASFSW010000030 DASFSW010000000
VERSION     DASFSW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16475
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.030"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..108)
                     /locus_tag="U1099_000775"
     CDS             complement(<1..108)
                     /locus_tag="U1099_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM4867110.1"
                     /translation="MSMFLDTAKIKVKAGKGGDGMVAFRREKYVPNGGPW"
     gene            complement(312..440)
                     /locus_tag="U1099_000776"
     CDS             complement(312..440)
                     /locus_tag="U1099_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEM4867111.1"
                     /translation="MAFGEEKHKKTTFEKVTLVIVVVMVVATLAGLILPAINALMN"
     gene            complement(497..2281)
                     /gene="uvrC"
                     /locus_tag="U1099_000777"
     CDS             complement(497..2281)
                     /gene="uvrC"
                     /locus_tag="U1099_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEM4867112.1"
                     /translation="MNDLIKHKLELLPDSPGCYLHKNKYGKIIYVGKAKNLRNRVRSY
                     FRGAHDTKTEALVAEIADFEFIVTDSNIEALLLEINLIQENKPRYNIMLKDDKSYPFI
                     KITKERHPRLIITRQIKKDGGQYFGPYPDVGAANQTLKLLERLYPFRKCKLPENKYCL
                     YYHLRQCLAHGDNMPSLSDYDKMVKEVAQFLTGHDDKIIKEVQDKMKAAAGNLEFEKA
                     AEYRDVLQSISTLRTKQRVMAHDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERNVNL
                     FPYYNDADEDFLTYIGQFYRDQQHLIPSEVLIPQDIDQEAVEALVDTKVIKPQRGEKK
                     QLINLATKNARVSLEQKFNLLEKNLEKTYGAVENIGQLLGIPTPVRIEAFDNSNIMGT
                     SPVSAMVVFENGVPNKKEYRKYKINTVEGPDDYASMREVLQRRYSRVLRDDLTPPDLI
                     IIDGGQGQVNVAKQVIEQELGMSIPIAGLQKNDKHQTHELLFGNPLEVVELPRNSQEF
                     FLLHRIQDEVHRFAIEFHRQVRSKNSFSSKLDGIEGLGPKRKQALMKHFKSIGNIQSA
                     SLQEIAEAGVPYKVAEKVKESLSSKDEI"
     gene            2521..3264
                     /locus_tag="U1099_000778"
     CDS             2521..3264
                     /locus_tag="U1099_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867113.1"
                     /translation="MIQFDHITKTYGETIALDDLNLTIESGEIFGLIGHNGAGKTTTI
                     SLLTSIIEASYGTISVDGLNLEENRDEVKKRIAYVPDSPDIFLHLTAFEYWHFMGKVY
                     GVEKDTVDDRIAKLSSLFDMTAHQHEPIDSFSHGMRQKTIIIGALIPNPDIWILDEPL
                     TGLDPQASFDLKQMMKEHAQSGHTVLFSTHVLSVAEQLCDRIAILRKGQLIFVGSLDE
                     LKSQYPDKDLETIYLELAGRQQVKAGEEV"
     gene            3261..4871
                     /locus_tag="U1099_000779"
     CDS             3261..4871
                     /locus_tag="U1099_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEM4867114.1"
                     /translation="MSKSIIWELVKINILYSNPQLLASVKKKQSKRKDASFSAYKSVL
                     LQQVFMVLSFGLLYSVFFMGVDYSQSIGFFSLQLALFTIIAIVYGFTGFFSVFYDSKD
                     TKLYLALPLKPQEVFLAKILSAQGMVLPFLMPCLSLLLITYWQIGGPLLALASIPSFL
                     ILWILANLVNLIIMHFVGEVLTKSPRKTLISTILMTGSSLLAFAALMFLQSQQTVSLE
                     SGGFVDFPIIPIFAGFHYLVSHTISLETLLHFGLPFILMIGLIVFAFKTVIPNYFNQV
                     LAIENASTKRTNNAKSAKIPSNLNQALVKHHLSTLKDSNLMVQTMIQPIIMGVALFPS
                     FSRFTENGGLSSVEWDFFGIALLAGFLLGSLFAGSTTFIGVAMSLEKENYHFIRTLPI
                     NFKKFIIQKFWVLAAVQFALPTLLYFALALGFMKVKLILAIFFTVGIIISALLTGQIY
                     YWRDQRLLMLNWQNSNQLFGRGAGQWLIAGSIIGTMFLGAIILVVSIILAGTIGAAYV
                     SSALMTLLFVLSAALQLYIYKAYWQKLK"
     gene            complement(4894..5350)
                     /locus_tag="U1099_000780"
                     /pseudo
     CDS             complement(4894..5350)
                     /locus_tag="U1099_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(5439..6194)
                     /locus_tag="U1099_000781"
     CDS             complement(5439..6194)
                     /locus_tag="U1099_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEM4867115.1"
                     /translation="MIMKRTILLCLPTILLLAACHQNINQANTTKNSSTVVSTTSSQS
                     ASTTSEQSTSSSTSAETNQEVTSTETQVNFNGSYYSVQGKYGEVIIVNKKHPLAADYA
                     PGENPEALAAFQRLIADMQALGFGISNNYSGFRSYETQASLYQSYVNQEGQAAADRYS
                     ARAGYSEHQTGLAFDLLDTSGNLLEEPNASQWLATNAHLYGFIVRYLPGKESSTGYMA
                     ESWHVRYIGQEATDIYNSGLTLEEYFGVPGGDY"
     gene            complement(6545..6796)
                     /locus_tag="U1099_000782"
     CDS             complement(6545..6796)
                     /locus_tag="U1099_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HEM4867116.1"
                     /translation="MKQIKNRLKKHMFWIGLISLVLMLVDNVLVCYFNIECSRTLNQI
                     EQTICIILSILVFLGVLNNKPDVKVEDIMKNGMSSKNQK"
     gene            complement(7039..7803)
                     /locus_tag="U1099_000783"
     CDS             complement(7039..7803)
                     /locus_tag="U1099_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4867117.1"
                     /translation="MKRLLRRIRPTKPLKELTWIDLIIITTILCGNAIYTSTMQWIAS
                     LSATETVETGVLSFSPADNWWALANQGKLFLFALVYLLIRNYDFKQLKVKLEWRVLLW
                     GPLIFIGAGLISDLAFTAFSYIPGLSGGYNFLGYLPYYDWNIMTVLNRFLAVDYSTVI
                     YSLFNGFYEEFFFLGLLLSTDKKKRSLVLLFSTIVRISFHTYQGMVSALVIGVAFGLF
                     YYYMYTRKNDNLLPYFLGHALADMVGTSFFSLFIAG"
     gene            complement(7882..8565)
                     /locus_tag="U1099_000784"
     CDS             complement(7882..8565)
                     /locus_tag="U1099_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEM4867118.1"
                     /translation="MQYKHLLFDLDHTLLDFSRGEEVALTQFLTAMEVEDIQAFKEVY
                     RPLNQGMWKDLEKGNITKKELINTRFSRTFAHFGRQVDGREMALRYQEFIGRQGQIFT
                     GADKLLQELTHRGYQIYAATNGVTYIQENRLLHSPIQSYFKEVFISEQMGTQKPAADF
                     YEKIAEQISGFQFDQALMIGDSLTADIQGGNNAGMDTVWYNPTHMINNSKAVPTYTIH
                     SYQELLDLL"
     gene            complement(8844..9584)
                     /locus_tag="U1099_000785"
     CDS             complement(8844..9584)
                     /locus_tag="U1099_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HEM4867119.1"
                     /translation="MSKRKIISSLFLSATVLGGLLFTQTPTVKAIELEDAQTMSTSSA
                     QAPIFSPGELIQETNYNDLVQKSVSIEDFSTNSNNEFTWKVKFDPTHWNFKHDKGGYY
                     FIIPEGMKLKKLVDKHTEKDLLTNFPENVNDSKNDSYSQYRHFKKGERTYWDRDFDSQ
                     WGWSAGRASNDKINQWKDENAFSDIYYIDSPRHAGPVTYELEAEVTDQNKTSFPLVAV
                     MKNFYARTSYLSEPTSLAGLDLKVEWPK"
     gene            complement(10026..12305)
                     /gene="pcrA"
                     /locus_tag="U1099_000786"
     CDS             complement(10026..12305)
                     /gene="pcrA"
                     /locus_tag="U1099_000786"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEM4867120.1"
                     /translation="MNPLLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKMVNPWNILAITFTNKAAREMKERAYALNPATQACLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRSLMKRILKNLNLDPKKWSERSILGTISNAKNDLIDDKAFEAQA
                     GDLYTQIVAKCYTAYQKELRQSEAVDFDDLIMLTLRLFDQNPEVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPESKVVL
                     LEENYRSTKNVLQAANEVIENNRNRRPKKLWTQNVQGDLITYYRARDENDEAIFVAGQ
                     IDQLHREGKAYKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVISY
                     LNIIANPSDNISYERIVNEPKRGVGPGTVDKLRDFATSHSMSLLEASQDIMLSPVKGK
                     AAQAVFDLANLLYNLRNQLDNLTVTQVVEAVLNQTGYIDALAAQNTLEANARIENIQE
                     FLSVTKNFDEKDVEEEETGLDKLTRFLLDLALIADTDDGDTESSEVTLMTLHAAKGLE
                     FPVVFLIGMEENVFPLSRAAEEEDELEEERRLAYVGITRAEQTLYLTNANSRLLFGRT
                     NYNQPSRFIREISSDLLDYQGLARPANTSFKASYVNGRATQFGQGMSLQQALQSRKAS
                     VQPSAKLGGSMPFASGNKSPGSGTNWSVGDIAVHKKWGRGTVLEVSGSGSNQELKINF
                     PEMGLKKVLASLAPISKEE"
     gene            complement(12595..13962)
                     /locus_tag="U1099_000787"
     CDS             complement(12595..13962)
                     /locus_tag="U1099_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM4867121.1"
                     /translation="MAKIVVVGANHAGTAAIKTMLTNYGQENEIVVFDQNSNISFLGC
                     GMALWIGEQIGGPEGLFYSNKEELESLGATVYMESPVTNIDYDAKTVTALVNGQEHVE
                     SFEKLLFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIEKLKNKDI
                     NRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLSDIMAKNLEEHGIK
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTALAGEGIERFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATGDTSYIALASNAVRTGIVAAHNICGTDLEGIGV
                     QGSNGISIYGLNLVSTGLTLEKATRLGLNAAVTEVTDNQKPEFMEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDISLGIHLFSLAIQEGVTIEKLALTDLFFLPHFNKPYNYITVA
                     ALGAE"
     gene            complement(14147..14485)
                     /locus_tag="U1099_000788"
     CDS             complement(14147..14485)
                     /locus_tag="U1099_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867122.1"
                     /translation="MQVIKRSGEIVEFDPDKIYQAIIKAAQTVYVIDETWRKNLAQVT
                     KKVVLDLEEAHTERPTISMVQSQVEHRLLDAGYISIAEHYISYRLQRDLERNGYGDKI
                     IVHLRFEQVR"
     gene            14652..16442
                     /gene="pepF"
                     /locus_tag="U1099_000789"
     CDS             14652..16442
                     /gene="pepF"
                     /locus_tag="U1099_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEM4867123.1"
                     /translation="MTNAMPKRQEIDVQLTWDTDILFPTPDNYKENLAAYAKQVTAFE
                     SNYKGKLTDKDTIVSALTEYEKIVILDSRLSHYAFLPLEVDKMNTELASLANEYDLVS
                     AKARPQRDFLYSELGQLDEAFLLELKDEQPQWAAFFDAILRDKPHQLHPLQEELLSNF
                     APTFGQPYNNYGVTKFEDMTFENFEANGQTLDNSYVLFENDYELSHDTEIRRNSAAGF
                     YSTLKKYKNTTAATYLSHIKNEQIEARLRGFDNTIDFLLHSQNVSRDLFDRQIDVIMK
                     ELAPHMRRYVKLVAKAHGLDKITHADLKISLPSEYNQRITPEESKQFLIDCLGILGED
                     YVKMIEQSFDERWIDFAQNEGKATGGFCATLYDGPSYILLSWTGLMNEVLVLAHELGH
                     AGHFQLAKKQSVLSYDPSLYFIEAPSTANEVLTCNTLLKNNQDPGFQAYLISELISRT
                     YFHNMVTHLLEAAFQREVYTRLDNEEYLNGDILCQIKLDVIKEFWGEDFEIGDDAGLI
                     WMRQPHYYIGLYPYTYSAGLTIGTAMAKQLEEHPEEVVEKWLETLSLGASLSAQDLAK
                     HAGVDVSTDQPLKETIAYVGSLVDKLETLI"
BASE COUNT         4721 a         3562 c         3111 g         5081 t
ORIGIN      
        1 ccaaggaccg ccgtttggta catacttttc acgacggaag gcaaccatcc catcgccacc
       61 tttccctgcc ttgaccttaa tcttggcagt atctaaaaac atactcataa tttacctatt
      121 ctttcctaga aaaaaacgct taaaagcgtt ttagacctct attcacaagc aaagtgctta
      181 tatataagaa aagtttttta gctaatcaag gtcgttcttt gagagaatac tgactgtatt
      241 ttgaaaaaac taacgatgaa cagcttaaaa accaacctta gataaaagtg ccgaactgta
      301 aatacaggtt cttaattcat caatgcatta atagctggta aaatcaagcc agccaatgtc
      361 gcgacaacca tcacgacaac gattactaaa gtcactttct caaatgttgt ttttttgtgt
      421 ttttcttcac caaaagccat tgctttctcc ttatttagtc attgatagtt tattctaaca
      481 cattatggga atgttttcaa atctcatcct ttgaagaaag gctttccttg accttttccg
      541 ccaccttgta aggcactcct gcttctgcga tttcttgtaa gctggctgac tggatattgc
      601 caatggactt gaaatgcttc atcaaagcct gcttgcgttt tggtcccaag ccttctatgc
      661 cgtccaattt tgatgagaag gaattttttg accgaacctg acgatgaaac tcaatagcaa
      721 agcggtgaac ctcatcttga atccgatgca ggaggaaaaa ttcctgagaa ttacgtggca
      781 actccaccac ttccagcgga ttgccaaaga gcaattcgtg ggtttggtgc ttgtcattct
      841 tctgcaaacc tgcaatggga atggacatac ccaactcctg ctcgatgacc tgcttggcga
      901 cattgacctg cccctgacct ccgtcaatga taatcaaatc aggcggtgtc aaatcgtccc
      961 tcaagacccg actataacgg cgttgcagca cttcacgcat agaggcataa tcatctggcc
     1021 cctcaaccgt attaatcttg tacttgcggt attctttttt attaggcacc ccattttcaa
     1081 agacaaccat agccgacaca ggactagtcc ccataatatt ggagttatcg aaagcctcaa
     1141 tccgcacagg cgtcggaatc cccaaaagct gaccgatatt ctccaccgca ccgtaggtct
     1201 tctccagatt tttctccaaa agattaaact tctgctccag actgacacgc gcattcttgg
     1261 ttgccagatt gatgagttgc ttcttctcgc cacgctgggg cttgatgacc ttggtatcta
     1321 ccagagcttc tacggcttct tggtcaatat cctgcggtat caagacctct gagggaatca
     1381 aatgttgctg gtccctgtaa aactgaccga tgtaggtcaa aaagtcttcg tccgcatcat
     1441 tatagtatgg aaaaagattg acattccgct caatcagttt cccctgccga acaaagaaaa
     1501 cctgcacaca catccagccc ttgtctacgt agtagccaaa gacatctcga ttctgcaagt
     1561 catgagccat cacacgttgc ttggtccgca gggttgaaat ggattggagg acatcccggt
     1621 attccgcagc cttttcaaat tccaaattgc cagcagctgc cttcatcttg tcctgcactt
     1681 ccttgataat cttatcatca tgacctgtca agaactgggc cacttccttg accatcttgt
     1741 catagtcaga caggctgggc atattgtctc catgagccaa acattgccgc aaatgataat
     1801 aaagacaata cttgttctca ggcaatttac acttgcggaa aggatagagg cgctccaata
     1861 gtttgagggt ctggttggcc gcacccacat ctggataggg accaaaatac tgaccaccat
     1921 ccttcttaat ctgccgagta atgattagtc gcggatgacg ttccttggta atcttgatga
     1981 agggataaga cttgtcatcc ttgagcatga tgttatagcg tggcttgttt tcctgaatca
     2041 gattgatttc tagcaagagg gcctcgatgt tggaatccgt cacgataaat tcaaaatcgg
     2101 caatttcagc cactagagcc tctgtcttgg tatcatgggc accacgaaaa taagaccgca
     2161 cccgattgcg taggtttttg gccttgccca catagataat cttaccgtac ttgtttttat
     2221 ggaggtaaca gccagggcta tctggtagta actccaattt atgcttgatc aaatcgttca
     2281 taggtctatt atagcaaatt ttctgataat cgagcttatt gcattccttt ttacactcga
     2341 cttcttgaaa tatatccgta tccatactac aaaaatattg atagaaacta ttgcttttgt
     2401 actaattcag cagtataata aatgtaatta attgtatata ttattcgaag aacatttgga
     2461 ataaggcttg gaatacctac aagcccctac ttttagttca cgaataacaa ggaggttatt
     2521 atgatacaat ttgaccatat cacaaaaaca tacggcgaaa caatcgcact tgatgaccta
     2581 aatttgacaa ttgaaagcgg cgagattttc ggtttaattg gtcacaatgg ggcaggaaaa
     2641 acaacgacaa tcagtctact gacttcgata atcgaagcta gttatggaac tatctctgtt
     2701 gatggtttaa acctagagga gaaccgcgat gaagtcaaaa aacggatagc ctatgttcca
     2761 gattcgccag acatcttcct acacttaaca gcttttgaat actggcactt tatgggcaag
     2821 gtctatggtg tagaaaagga tacagttgat gaccgtatcg ctaaactatc tagtctattt
     2881 gatatgacag cgcaccagca tgagcctatt gatagttttt cacacggtat gcgtcagaaa
     2941 accattatta ttggtgcatt gatccctaac cctgatattt ggattttgga tgaaccccta
     3001 actggactgg atcctcaggc ttctttcgac ctaaaacaaa tgatgaaaga acatgctcaa
     3061 tctggtcaca ccgttctatt ttctacacac gtcctttcag tagctgaaca actctgtgac
     3121 cgcattgcta ttctccgaaa agggcaactc attttcgtag gaagtcttga tgaactcaaa
     3181 tcacagtatc cagataaaga tttggagacc atctatttgg aattagccgg acgccaacaa
     3241 gtgaaggcag gtgaggaagt atgagtaaat ccatcatttg ggaactagta aagattaata
     3301 ttctttattc caatcctcaa cttctagctt ccgtcaagaa gaaacaaagt aagcgcaaag
     3361 acgcctcctt ttcagcctat aaaagtgtcc ttttgcaaca agtatttatg gttttgagtt
     3421 tcggacttct ctattctgtc tttttcatgg gagtagatta tagtcagtct atcggtttct
     3481 tttctctgca actagcgctc tttaccatta ttgccattgt ctacggtttt acaggtttct
     3541 tctccgtttt ctacgatagt aaggatacaa agttatattt agcacttccc ctgaagccac
     3601 aagaagtctt cctcgctaaa atcctctctg cacaagggat ggtcctaccc ttcctcatgc
     3661 cgtgtctgtc tttacttcta atcacttact ggcaaattgg cggtccgctc ctcgccttag
     3721 cttccattcc ttcctttttg attctctgga ttttagctaa tttagtcaat ctgattatca
     3781 tgcactttgt tggggaagtt ctgacaaaaa gccctcgtaa aacattgatt tctacaatcc
     3841 tgatgacagg ttcttccttg ctggcttttg ctgcgctcat gttcctgcaa tcccagcaga
     3901 ccgtttcact cgaatctggt ggctttgttg attttcctat catacctata tttgctggat
     3961 tccattactt ggtcagccat actatatcac tagaaacact ccttcatttt ggacttcctt
     4021 tcattttgat gattggactg attgtgtttg cgtttaaaac tgtcattccc aactacttta
     4081 atcaagtttt ggcgattgaa aatgcttcga caaaacggac caataatgct aagtctgcca
     4141 agattcctag caatctcaat caggctttag tgaaacacca cctatctact cttaaagata
     4201 gcaacctcat ggtccaaacc atgatacaac caatcatcat gggagttgcg ctttttccaa
     4261 gttttagccg tttcacggag aatggtggtc taagttcagt tgaatgggat ttctttggta
     4321 ttgctctatt agctggattc ttgcttggca gtctatttgc tggttctaca accttcatcg
     4381 gagtagccat gtcattggaa aaggaaaatt atcatttcat tcgaacactt cctataaatt
     4441 tcaaaaaatt tatcatccaa aaattctggg tattggctgc cgtacaattc gcactaccaa
     4501 ctctacttta ctttgctttg gctctaggtt tcatgaaagt caaactcatt ctagcaatct
     4561 tctttacagt cggcatcatc atttcagccc tgctaactgg tcagatttat tactggcgtg
     4621 accaacgact cctcatgctc aactggcaaa atagtaacca gttatttggt cgcggagcgg
     4681 gtcaatggct aattgctgga tcgatcatag gaacaatgtt tctcggagcc attattctag
     4741 ttgtcagcat catattagct ggcactattg gtgcagctta tgtaagctca gccttgatga
     4801 ccttactatt tgttctatct gctgctcttc aactttatat ctacaaagct tattggcaaa
     4861 aactaaaata gattggacat tgtccaatct attttagttt tctgcgtaat attcctgacc
     4921 gtagccttct acttcagcat tgatttgcca agagatacca aacttgtctg ttacattacc
     4981 ataggcagga gaccaaggaa ctgcttgaag ttccatcttg aagttccata ttgatagcag
     5041 ttgcatctac ggataacttg ctgtatagtt cctgcgctgt ctcagcatcg tctgtgataa
     5101 gcgcaactgt aatattgtca cctactgtat aaggcatttc tgggttgttg tctgacaaca
     5161 taaaacgctg accattcaaa cgaaactgtg cattcatcac gtaattttct agttcagcag
     5221 ggcagtctgg taagaaatct ttaaatagat ttacttgctc aactgttgcc ccaagagcat
     5281 ttttataaaa ctcgatggct tccaaaccat taccattagt aaccaagtat acttccattg
     5341 ctttaagcat gatggacctc caaaattatg atggtttatt atatcacgag ttagttatac
     5401 ggacaaattt ttcggcttat ttctgactaa atgtaaaatt agtagtctcc acctggtaca
     5461 ccaaaatatt cttccaaggt caagcctgaa ttgtaaatat ctgtcgcttc ttgaccaata
     5521 tatcgcacat gccaggattc tgccatatag cctgtgctag actcttttcc tggaagatag
     5581 cggacaataa agccatatag atgtgcattc gtagccaacc actgagaagc attaggctct
     5641 tctaataaat taccacttgt atccaataaa tcaaaggcta atcctgtctg atgttcacta
     5701 tatccagctc ttgcagaata cctatctgcc gctgcttgtc cttcctgatt tacataagac
     5761 tggtagagac ttgcctgagt ttcataggaa cgaaatccac tatagttatt ggagatacca
     5821 aatcctagcg cctgcatatc tgctattaat cgttgaaaag ctgctaaggc ttcaggattt
     5881 tcgcccggtg cataatcagc tgcaagcgga tgctttttat ttacaataat cacttcgcca
     5941 tattttcctt gtacactata ataggaaccg ttaaaattta cctgagtctc tgtacttgtt
     6001 acttcttgat tcgtttccgc cgatgtagag ctgcttgttg actgctccga agttgttgaa
     6061 gcagactgac ttgaagtagt cgaaacaact gttgacgaat tttttgtagt attggcctga
     6121 tttatatttt gatgacatgc tgctagtaac agtattgtcg gcaagcataa tagaatagta
     6181 cgtttcatta tcatcacccc gttctcttat aattatgtct attgtagcac aatcataaga
     6241 ccccgtctaa tgacggctta tgattttaac tactattgat atttttagta ttcattgaat
     6301 ttctctcgat tcttttcctt tcaaatagtg gcattcctga taacatacat gtcataaata
     6361 gaaacaccag cctttcgact ggtggacata tatgatgtta aatagtcgta ttcacaagta
     6421 aagtgattta tatacaaaat agttttttag gagatactga ctatattttg aaaaatccat
     6481 cttagctaga gatactgaac tgtaagtaca actagatacc tcatcagtag tttctaaaca
     6541 aacattattt ttgattttta gatgacatac cgtttttcat gatgtcttct accttcacat
     6601 ccggtttatt atttagcact cctaaaaata caagaatact gaggataata caaatagtct
     6661 gctcgatttg gtttaaagta cgactacatt caatattgaa ataacaaacc aggacattat
     6721 caacgagcat caataccaac gaaattagtc caatccaaaa catatgtttt ttcaaacgat
     6781 tctttatttg tttcatatct atactccttt ttgatgtata aaaatacatt ttaacataaa
     6841 taaaccatta attcaaaaaa atatgggata aaaaatatga taaagcaatc gaaatttgac
     6901 tgaacagatt aaatatatca gcacaataaa acgggtgatg ataccgagtt gatagaaact
     6961 acgtatcatc atccgtttgt cagctgtagg gagaaagaaa ttttatccga ttagcttgat
     7021 gtccgttctt ctcattgttt accctgcaat aaacaatgag aagaaacttg taccaaccat
     7081 atccgcaaga gcatgtccta agaaatatgg caataaattg tcatttttcc ttgtgtacat
     7141 gtagtaatag aaaaggccga aggctacacc aataacaaga gcagatacca ttccttgata
     7201 ggtatggaaa gatatacgga caattgttga aaagagtaag accagcgaac gctttttctt
     7261 atctgtactc aacaataagc ctaagaaaaa gaactcttca tagaagccat taaacaagga
     7321 atagataact gtgctgtagt caacggctag gaaacggttg agcactgtca ttatattcca
     7381 gtcatagtaa ggcaagtatc ctaggaaatt atacccacca gacagacctg gaatatatga
     7441 aaaagctgta aatgccaaat cgctaatcag accagcaccg atgaaaatca gtggtcccca
     7501 gagcaaaact ctccactcta attttacttt tagctgcttg aaatcataat tccgaatcaa
     7561 taaatacacc agggcaaaca ggaataattt tccttgattg gctagagccc accaattatc
     7621 ggctggacta aacgacagaa ctcctgtttc aacggtttct gttgcggaaa gtgatgctat
     7681 ccattgcatg gttgaggtat aaatagcatt accacataaa atcgttgtga taataatcaa
     7741 atctatccaa gtgagttctt ttaggggctt tgttggacga atgcgtctca gtaatctttt
     7801 cactttttcc tccttatatt tttgtacata ttctttcggt ataattatat actaaaatcc
     7861 ttataagttt cttaaaatat cttataacaa atctaataat tcttgatagc tatgaatggt
     7921 ataggttgga actgccttac tattatttat catatgagtc ggattatacc aaacagtatc
     7981 catgccagca ttattgccac cttgaatatc tgctgtcaaa ctgtctccaa tcattaaggc
     8041 ttggtcaaac tgaaaacctg aaatctgctc tgctattttt tcataaaaat cagccgcagg
     8101 cttttgcgtc cccatctgct cagaaataaa tacttcttta aaatacgatt gaatcggtga
     8161 gtgaagcaag cgattttcct gtatataggt cacgccgttg gtcgctgcat aaatctggta
     8221 gcctcggtgt gtcaattcct gcaaaagctt atctgcacct gtaaaaatct gtccctgccg
     8281 cccaataaat tcttggtagc gcaaggccat ctcccttcca tcaacttgcc gaccaaagtg
     8341 ggcaaaggtc cgtgaaaagc gggtgtttat tagctctttc tttgtaatgt tccctttttc
     8401 caaatccttc cacatgccct ggttgagcgg tcgatagact tctttaaaag cttgaatatc
     8461 ttcaacctcc atagctgtca aaaactgggt taaggctact tcttctccac ggctaaaatc
     8521 aagaagagta tggtcaagat caaagagtaa gtgtttgtat tgcaagatga taggtccttt
     8581 ctaagcagaa actactgaaa cgatgtcagg cagtctatct aaagtatacc acagtttcta
     8641 aaaaaaatag ctccgtgtcc taggctctat tatactgttt ttcagatttc agtagctctg
     8701 gacgtacggg tttcctcatc atattatgca agcgctagtt tattcaagta aagagaagag
     8761 gactcgcaga aaaaggcaat cgtagtagcc ttaactacac acgattgcca ctggttggtt
     8821 ttgttctgca aattgtcagt cacttatttc ggccattcaa ctttcaaatc aagtcctgca
     8881 agcgaagttg gttcactcaa gtatgatgtt ctagcataga agtttttcat taccgctacc
     8941 agagggaagg aagttttatt ttgatcagtt acttctgcct ccaattcata agttactgga
     9001 cctgcatggc gaggactatc gatataatag atgtcagaaa atgcattttc atctttccac
     9061 tgatttatct tatcattact tgctcgtcca gctgaccatc cccactgact atcgaagtca
     9121 cgatcccaat atgttctttc gcctttttta aagtggcggt actgactgta tgagtcgttc
     9181 ttactgtcat taacgttttc cgggaagtta gttaataagt ccttttccgt atgtttgtca
     9241 acaagtttct tcaatttcat accttcagga ataataaaat aatatcctcc cttatcatgc
     9301 ttaaagttcc aatgtgtagg atcgaacttt actttccatg taaactcatt attgctattt
     9361 gtcgaaaaat cctcaataga tactgacttt tggaccaagt cgttatagtt agtctcttga
     9421 ataagttccc caggagaaaa aatcggtgct tgtgcgctgc tagtactcat ggtttgtgca
     9481 tcctctaact caattgcttt tacggttggt gtctgtgtaa acagcaatcc tcccaatacg
     9541 gtagcactta aaaataaact tgatataatt ttacgtttgc tcataagagt ctccttcttt
     9601 tatttgatct atttttgcgc tagctacaaa aatattggca agctaatcgt cccatttcta
     9661 ggctatccct tccttactaa gatgagagtc tctagcaaat aaaagacttt taactactat
     9721 cagtataaca atacaagtag tattttctct tggagctatc cgtttttttc tatatttgtg
     9781 gtttagcata tggatggtaa gacacactct aaaaacatcc gattcaaaat tcaaaatttg
     9841 cttatctaga tgtcatcaaa gcgtgcaaga ttgtttcgat gctttaagca gtatacaaaa
     9901 aatcacctca aaacagtcag gtcaaaactc attttgaggt gtcatatatc gttctaaaaa
     9961 taatattatg aagacgtcgc aagtcttgta aaatgcgatg tttcctcttt acttttgatt
    10021 catccctact cttccttact aatcggtgct aagcttgcca agactttttt gaggcccatt
    10081 tctgggaaat tgattttgag ttcttggttg ctgcctgaac ctgatacttc aaggactgtc
    10141 ccacgtcccc atttcttgtg gactgcgatg tcaccaactg accagttggt gcctgagcca
    10201 ggcgatttgt tgcctgatgc gaaaggcata ctgcctccga gcttggcaga tggttgtacg
    10261 gatgcttttc gactttgtag ggcttgttgc aagctcatgc cttgtccaaa ctgtgtcgct
    10321 ctgccattga cataagaagc cttaaagcta gtattagctg gacgagcgag gccttgataa
    10381 tcaagcaagt cactggaaat ttctcgaata aagcggcttg gttggttgta gttggtgcga
    10441 ccgaaaagga gtcgagaatt ggcattggtc aggtagagag tctgctcagc acgggtgatg
    10501 cctacgtatg ccaaacgacg ttcttcctcc aattcgtcct cttcttctgc tgcacgacta
    10561 agtgggaaga cattttcctc cataccaatc aagaagacaa ctggaaattc cagacctttg
    10621 gctgcgtgga gggtcataag agtcacttct gaagattctg tatcgccatc atctgtgtca
    10681 gcaatcaggg ccaaatcaag caggaagcgt gttaatttat ctagacctgt ctcttcttcc
    10741 tcaacatcct tttcatcaaa gtttttggta acagaaagga attcctggat gttttcaatg
    10801 cgggcatttg cttccagggt attttgtgct gcaagggcat cgatgtagcc tgtttggttc
    10861 aatactgcct cgactacttg ggtaaccgtc aagttgtcta attgattgcg aagattatag
    10921 aggagattag ctaagtcaaa gactgcctgt gctgccttgc ccttgactgg tgacaacata
    10981 atatcctgag aagcttctag caaggacatg ctgtggctgg tcgcaaaatc acggagtttg
    11041 tcaaccgtac cagggccaac gccacgtttt ggctcattga caatgcgttc ataagagata
    11101 ttatccgatg gatttgcgat gatattcaaa taagaaatca catctcgaat ttccttacgg
    11161 ctgtagaact tggtgccgcc aaccatggta taagggatat tagatttgag cagggcttct
    11221 tcaatggtac gggattgggc attggtacgg tagagaactg caaaatcctt ataggcctta
    11281 ccttctcggt gcaactggtc aatctgccct gcgacaaaaa tcgcctcgtc attctcatct
    11341 ctggcacgat agtaggtaat caaatcccct tggacattct gtgtccagag ttttttggga
    11401 cgtcgattgc ggttgttttc gatgacctca ttggcagcct gcaagacgtt cttggtcgaa
    11461 cgatagtttt cctccaaaag cacaaccttg ctctctggat aatccttttc aaagtccagg
    11521 atattttgca tatccgcacc acgccaacca tagatagact ggtccgcatc accgaccacg
    11581 cagatattct tgaaacgtga tgccaggagc ttgaccaatt ggtactgggc atggttggta
    11641 tcctgatact catcgacatg gatatactgg aatttctgct ggtaataggt caggacctct
    11701 ggattttggt caaaaaggcg gagcgtcaac atgatcaaat catcaaagtc aacggcctct
    11761 gactgtctta gttccttttg gtaagccgtg tagcatttag caacaatctg cgtgtatagg
    11821 tcgcctgcct gggcttcaaa cgccttgtca tcaatcaagt cgtttttggc attagaaatg
    11881 gttcccagga tagaacgttc actccacttt ttcggatcta gattgagatt ttttaaaatc
    11941 cgtttcatga gactgcgttg ctcacctgga tcgacaatcg taaaattgcg attgtaaccg
    12001 atatggtcag catcccgacg caaaatccgc acacacatgg agtggaaggt agctatcaaa
    12061 caggcttgcg tggcaggatt gagggcatag gcccgctcct tcatctcccg tgccgccttg
    12121 ttggtaaagg taatggctag gatattccaa ggattgacca ttttttcatc aatcaaataa
    12181 gcgatacggt gggtcaggac tcgcgtctta cccgaaccag cccctgccat aatcaaaagc
    12241 ggaccttccg tcgtctgtac cgcttctgcc tgtctgtcat tcattccgtt taataatggg
    12301 ttcattttct catactttcc tagaacatgt atttacaagt aattcacttt caaataggaa
    12361 atcgtttata gctaatcaag gtaatttttt gaggaaatac cgactgtatt tttggaaaaa
    12421 caaacgataa atagctgaaa aactaggctt agatgaaagt gctaaactgt aaatacaggt
    12481 tcattataat cataccctcc attataccaa aaattctctc gataggcagg tgcaaaatga
    12541 tggagaacag ccaaaaaagc ccagagtcat ctctgagctt ttgtattcaa ttgattattc
    12601 agcacccaag gcagctactg tgatgtagtt gtaaggtttg ttgaagtgtg gcaagaagaa
    12661 caagtctgtc aaggccaatt tttcgattgt tacaccttct tggattgcca atgagaagag
    12721 gtggataccc aatgagatat cttcacgagc agccatttgc gcaccaagga tacgacgtga
    12781 atctttatcg taaacaatct tgatagttac tgggaagtta ccgtgctcca tgaattctgg
    12841 tttttggttg tctgttactt cagtaacagc agcgttcaaa ccaagacgag tagctttttc
    12901 aagtgtcaaa cctgttgata caaggttaag accgtagata gagataccgt tagaaccttg
    12961 tacaccgata ccttcaaggt cagtgccaca gatgttgtga gctgctacga taccagtacg
    13021 tactgcgttt gaagccaaag cgatgtagct tgtgtcgcca gtagcgttgt cgtagattgt
    13081 tgcacagtca ccgatagcgt aaacgcctgg gattgaagtt tcttggcgtt tgtttacaag
    13141 gaaggcaccg ttacggaagc gctcgattcc ttcacctgca agcgcagtgt ttggacggaa
    13201 accaacagcc aagataacca tgtcaacatc gtattcgttc ttgtcagtga tgattttctc
    13261 aactttacca ttaccagcaa cttctttaac agtttcgccg aaggcaagtt tgataccatg
    13321 ctcttccaag tttttagcca taatatctga caagtcacgg tcatagtacc cagccaaaca
    13381 tgtatccaca acatcaataa gtactacttc tttaccttta cgttggaatg cttccgcaag
    13441 ctcaacaccg atgtaaccag caccaacaac tgcgacgcgg ttgatgtctt tgtttttcaa
    13501 tttttcaata acatctgctg agttttggta caatttaaca aattgcaagt tttcaagagt
    13561 tgcttcaaat tcaagcgaac cttctttgat ttcagctcct ttgattgggg gtaagatagg
    13621 ttgtgaacca gttgcgaaaa gaagtttttc aaatgattca acatgttctt gaccatttac
    13681 aagcgctgta actgttttgg catcatagtc aatgtttgta acaggtgact ccatgtatac
    13741 tgtcgcaccc aagctctcca actcttcttt gtttgagtag aaaagtcctt caggaccgcc
    13801 aatttgctca ccgatccaca aagccatacc acaacccaag aatgaaatat ttgagttttg
    13861 gtcaaataca acgatttcat tttcttgacc ataatttgtc aacatagttt tgattgcggc
    13921 agtaccagca tggttagcac cgacaacaac gattttagcc ataatgtaaa atctcacttt
    13981 cgaaaatata tttttttacg aactatatta tacaatataa atgttaccga tttcaaaaaa
    14041 tatgctcaga atatcacatt tttttcaaaa agtttttgaa atgctatttt ctttaaacaa
    14101 tgtaaagata tataaaagca aaaacctaga tttctctagg tttctcttaa cgtacttgtt
    14161 caaaacgcag atgtacaata atcttatcgc catagccgtt acgttctaaa tcacgttgca
    14221 agcgatagga aatgtaatgc tcagcaatag aaatataccc tgcatctaaa agacgatgtt
    14281 caacttgcga ctgaaccatg ctaatcgttg gtcgctctgt atgagcttct tccaagtcaa
    14341 gcactacttt ctttgtgacc tgagcaagat ttttccgcca agtttcatca atgacataga
    14401 ctgtctgtgc agctttaatg atggcttggt agattttatc tggatcaaat tcaacaatct
    14461 ctccactacg tttaatgact tgcataaaag ctcctttttg gttttcagta aaccctttca
    14521 tacttacatt atctaggttt ttgtgtattt tgtcaagtat tctttttcat aaattttgcc
    14581 ttggcttatt ttcgctgaaa gccctttagt atgatataat ggacctatta aaatgaaata
    14641 aggagaaaat catgacaaat gcaatgccta aacgtcaaga aattgatgtg caattaactt
    14701 gggatacgga cattctattt ccgacccctg ataactacaa agaaaatttg gcagcctacg
    14761 ctaagcaagt gacagctttc gaatcgaatt acaaaggaaa gctaactgat aaggatacca
    14821 tcgtttccgc cttaacagaa tacgaaaaaa tcgttattct tgatagcaga ctttcccact
    14881 atgccttcct tcctttggaa gtcgataaga tgaatactga attggcgagc ctggccaacg
    14941 aatacgacct cgtttctgcc aaggcacgtc ctcaacgaga tttcctctat tcagaacttg
    15001 ggcaattaga tgaagccttt ctacttgaat taaaagatga acaaccacaa tgggctgctt
    15061 tcttcgatgc tattctccgt gacaagccgc atcagcttca tccactacaa gaagaacttt
    15121 tatcaaattt tgctccaaca tttggtcagc cctataacaa ctacggtgtg accaagtttg
    15181 aagatatgac atttgaaaac ttcgaggcaa atggtcaaac gctcgacaat agctatgtgc
    15241 tctttgaaaa tgattatgag ctcagccacg ataccgaaat ccgtcgcaat tcagctgctg
    15301 gtttctattc aaccttgaag aaatacaaga atacaactgc agcaacttat ttgtctcata
    15361 tcaagaatga gcaaatcgaa gctcgcttgc gtggatttga caatacgatt gacttcctct
    15421 tgcatagtca aaatgtctct cgtgatctct tcgaccgtca gattgatgtc atcatgaaag
    15481 aattggctcc tcacatgcgt cgctatgtaa aattggttgc taaggctcat ggattggaca
    15541 agatcacaca tgctgacttg aaaatcagtc taccatctga atacaaccaa cgcatcacac
    15601 ctgaagaatc caaacaattc ttgattgact gcctgggcat tcttggtgaa gattatgtga
    15661 aaatgattga acaatcattt gatgagcgtt ggattgactt tgctcaaaac gaaggtaagg
    15721 ctactggtgg cttctgtgct actctttacg atggaccatc ttacatccta ctttcatgga
    15781 caggcttgat gaacgaggtc ttggtattgg ctcacgaatt gggacatgct ggtcatttcc
    15841 aattggctaa gaaacaaagt gtccttagct acgatccatc cctctacttc atcgaggcgc
    15901 cttctactgc aaatgaagtc ttgacatgta acaccctttt gaaaaacaac caagaccctg
    15961 gcttccaggc ttacttgatt agcgagttga tcagccgtac ttacttccac aacatggtca
    16021 ctcacttgct tgaagcagcc ttccaacgtg aagtctacac tcgtttggac aatgaagaat
    16081 acctcaacgg tgatattctc tgccaaatca agttggatgt catcaaggaa ttctggggtg
    16141 aagactttga aatcggtgac gatgcaggtc ttatctggat gcgtcaacca cactactaca
    16201 ttggtttgta tccatatacc tactcagctg gtttgaccat cggtacggct atggctaagc
    16261 aattggaaga acatcctgaa gaagttgttg aaaaatggtt ggaaaccttg tcactcggtg
    16321 caagtctttc tgcccaagac cttgctaagc acgcaggtgt tgacgtttca actgaccaac
    16381 cactcaaaga aaccattgcc tatgttggtt cattggtgga taaactggaa acactaatct
    16441 aagacacata aaagacttga acaacttgtt caggt
//
LOCUS       DASFSW010000031        16009 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.031, whole genome shotgun sequence.
ACCESSION   DASFSW010000031 DASFSW010000000
VERSION     DASFSW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.031"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(51..1187)
                     /gene="dnaJ"
                     /locus_tag="U1099_000790"
     CDS             complement(51..1187)
                     /gene="dnaJ"
                     /locus_tag="U1099_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEM4867124.1"
                     /translation="MNNTEFYDRLGVSKNASPDEIKKAYRKLSKKYHPDINKDPGAED
                     KYKEVQEAYETLSDPQKRSAYDQYGPAGANGGFGGGAGGFGGFDGAGFGGFEDIFSSF
                     FGGGGATRNPNAPRQGDDLQYAVNLKFEEAIFGVEREVSYNREATCRTCTGSGAKPGT
                     SPVTCGRCHGSGVINVDTQTPLGTMRRQMTCDVCHGRGKQIKDPCATCHGTGHEKQAH
                     TVTVKVPAGVETGQRIRLAGQGEAGFNGGPYGDLYVVIQVQPSDKFEREGTTIHYKLN
                     LNFVQAALGDTVHVPTVHGDVDMVIPEGTQTGKTFRLKGKGAPSVRGGAIGDQYVTVN
                     IVTPTGLNDRQKAALKEFAAAGNIDVKPHKKGFFDKVKDAFEDL"
     gene            complement(1721..3544)
                     /gene="dnaK"
                     /locus_tag="U1099_000791"
     CDS             complement(1721..3544)
                     /gene="dnaK"
                     /locus_tag="U1099_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEM4867125.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     EYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDHMVAEFKKEN
                     GIDLSVDKMALQRLKDAAEKAKKDLSGVTSTQISLPFITAGAAGPLHLEMTLTRAKFD
                     ELTYDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRND
                     VDQAIFATEKTLKETEGKGFDAERDQAQAALDELKAAQEANNLDDMKAKLENLNEKAQ
                     ALAVKLYEQAAAAQQAAAGQEGAQTANNAGDDVVDGEFTEK"
     gene            complement(3633..4145)
                     /gene="grpE"
                     /locus_tag="U1099_000792"
     CDS             complement(3633..4145)
                     /gene="grpE"
                     /locus_tag="U1099_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEM4867126.1"
                     /translation="MSEEIKNEEIVEEVEATEEVVETPEKSELDLANERAEEFENKYL
                     RAHAEMQNIQRRANEERQTIQRYRSQDLAKKILPSLDNLERALQVEGLTEDVKKGLEM
                     VQESLIQALKEEGVEEVATDVFDPNLHMAIQTVPATDDCPAEHIAQVFQKGYKLHERL
                     LRPAMVVVSE"
     gene            complement(4282..5316)
                     /gene="hrcA"
                     /locus_tag="U1099_000793"
     CDS             complement(4282..5316)
                     /gene="hrcA"
                     /locus_tag="U1099_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEM4867127.1"
                     /translation="MITQRQNDILNLIVELFTRHHEPVGSKALQEMIASSSATIRNDM
                     AKLEQLGLLEKAHTSSGRMPSRAGFQYFVNHSLNLEHIDEEDVYQVVKAFDFEAFKLE
                     DILERASTVLADLTGYTSVILDVEPTSQQLTSFDIVQLSSHDALAVLTLDQSKPVTVQ
                     FAIPKNFLTRDLEVLKRLVDERFVDQTVLSIHYKLRTEIPQVVQRYFATTDNVLDLMD
                     YIFSNLFQESVFISGKVASLTYGNLATYQLLDSAQLLAPELRQGLAPDQQTSISVAEH
                     SDSALADVTVIHHRFPIPYRGMGQMSLLGPVDMNYRRQMSLINIISRVLFMKLTDYYR
                     YLSSNHYEVN"
     gene            complement(5538..6233)
                     /locus_tag="U1099_000794"
     CDS             complement(5538..6233)
                     /locus_tag="U1099_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM4867128.1"
                     /translation="MADVRLYISRHGKTMFNTIGRVQGWCDTPLTKAGEEGIRELGLG
                     LKDAGLEFKLAVSSDLGRTVQTMTIAQRELGILGKIPYYQDKRIREWCFGSFEGMYDA
                     ELFQGVLPRLKGTVDATGMTFAEIAAGIQEADTAGWAESWEVLSNRILTGFEAIAQDL
                     KKQGGGNALVVSHGMTIATLAHLLEPERGANIFLDNGSITVLKYENGKLLIEAVGDLS
                     YRKRGAELIAQGK"
     gene            complement(6309..7646)
                     /locus_tag="U1099_000795"
     CDS             complement(6309..7646)
                     /locus_tag="U1099_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEM4867129.1"
                     /translation="MDIRQVRETIEMIEEQNFDIRTITMGISLLDCIDADIEKAAEKV
                     YTKIVTKAKNLVAVGDEIAAELGIPVVNKRVSVTPIALIGAATDATDYLPLAHALDKA
                     AHEIGIDFIGGFSALVQKGYQKGDEILINSIPQALAQTTKVCSSVNIGSTKTGINMTA
                     VRDMGRIIKETAEASDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVVINVGVSGPGV
                     VKRALEKVRGESFDVVAETVKKTAFKITRIGQLVGNMASERLGVKFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDAVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPETTPAETIAAMIADEAAIGVINQKT
                     TAVRIIPLGKEGDMIEFGGLLGTAPVMKVNQASSADFINRGGQIPAPIHSFKN"
     gene            complement(7658..7924)
                     /locus_tag="U1099_000796"
     CDS             complement(7658..7924)
                     /locus_tag="U1099_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEM4867130.1"
                     /translation="MKAIVTVVGKDKSGIVAGVATKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKKDFTKLRAELEAFGQGLNVKINIQSAAIFDAMHNL"
     gene            8181..9923
                     /locus_tag="U1099_000797"
     CDS             8181..9923
                     /locus_tag="U1099_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM4867131.1"
                     /translation="MKIIKQLWWFFSLEKKAYLIGILSLCLVSLLNLLPASIMGQLID
                     QIASGQLTEHRLLLGVAGLILSALAMYGLRYLWRMNILATSYRLGKIMRARLFNHFMH
                     LSPSFFQQYRTGDLMAHATNDINALTRLAGGGVMSFVDATVTALVTLVTMSVSISWQM
                     TLVAILPLPFMTLTTNFLGRRTHENFKASQAAFSELNNKVQEAVSGIKVTKSFGYQEQ
                     ETAAFQEVNQAAFLQNIKTMRYDALFNPAVLFFIGLSYLLTLLVGSHFISQGQVSLGQ
                     LVTFMTYLDLLVWPLMAIGFLVNISQRGDVSYNRIQTLLSISSEVIETDQPLPAPSNG
                     EIVYEISEFAYDNIPVLQDIQFHIKKGQTIGLVGPTGSGKTSLLKLLMREYDVTNGQI
                     LLNQQNIKHYKLGDLRQLMGYVPQDQFLFATSVAENIRFGNPDLSLDQVKEAAKAVHV
                     YEDIQDMPDRFDTMVGEKGISLSGGQKQRLAMARAMILNPDILLLDDSLSAVDAKTEH
                     AILETIKQERLDKTTIITAHRLSAIVHADLILVLEDGKIVERGRHQELLDEKGWYYDT
                     YMMQQLEKEESDAL"
     gene            9913..11661
                     /locus_tag="U1099_000798"
     CDS             9913..11661
                     /locus_tag="U1099_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867132.1"
                     /translation="MLSKTKQASVFWRLLSYLKAYRLLTVLALIFLLLTTVVRSLIPL
                     LASYFIDHYISSISETAAAILAAYFALYLLQMVLQYFGNLWFAKVSYSIVRDIRRDAF
                     GRMEKLGMAYFDQTPSGSIVSRITNDTESISEMFSGILSSFISALFIIITTLSTMFAL
                     DWKLTCLIVLFLPLIFLLVDQYRKRSAPIVAKTRSLLSAINSKLAESIEGIRIIQAFS
                     QEERLKEEFEAINQEHLDYASRSMALDSLFLRPALSLVKILAYALLMTYFGLDWSQAG
                     ISAGLIYAFIQYVNRLFDPLLDVTQHFSTLQTSMVSAGRVFTLMDQTFEEPLQLDRGA
                     KIDKGHIVFEDVCFSYDGKRQILDKVSFEVRQGQTIAFVGATGSGKSSIINVFMRFYE
                     FQSGRILIDGQDIRSFSQEELRSAIGLVLQDPFLFHRTIASNIQMYQDISREEIEEAA
                     RFVDAAPFIEKLPEKYDHLVTERGTAFSTGQRQLLAFARTMASQPKILILDEATANID
                     SETEQTVQTSLEKMRKGRTTIAIAHRLSTIQDADCIYVLDKGKIIESGNHEELLALKG
                     HYHRMYQLQAGQLTAT"
     gene            complement(11756..12853)
                     /locus_tag="U1099_000799"
     CDS             complement(11756..12853)
                     /locus_tag="U1099_000799"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEM4867133.1"
                     /translation="MFYLGEFGLDRKDQHVGLANQQYSATPAKDFTETLFVHHSLPQT
                     KVDEVDISTSVAGLDFAFPFFINAMTGGSKKTREINRLLGIMGHFGKIALASGSVSAA
                     IKDPSVAETFSVMRRENPYGIIFANLGAHHSVENAKRAVDLLEANAIQIHVNAPQEIV
                     MPEGDRDFTMWLKNIETLVREMEVPVIVKEVGFGMSRETVAQLASVGVQTIDVSGTGG
                     TDFAKIENARRTFNDYTYLEGWGQSTVTSLVEAMSVSEEVRPSLIASGGIKTPLDIVK
                     SLALGADLVGMSNHFLQYVKDGKGHRFDDGLQAIKTYQWQMAEIMTMLGAKNIAELRQ
                     KDLVLAPNIQNWCEARGIDWKAYARRSANVS"
     gene            complement(12864..13943)
                     /locus_tag="U1099_000800"
     CDS             complement(12864..13943)
                     /locus_tag="U1099_000800"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEM4867134.1"
                     /translation="MKVQVKIPGKLFLAGEYAVVEAGYPAVIAAVDQYLTVTIETSDR
                     GLLHSSQQVDLYLIWERKEGIAHVQGDHPYALIETVMQVTEAYLTAKGYACHGTYSLA
                     VQSDLDDQTSGAKYGLGSSGAVTVATVKALLTYYGHRPDALLTYKLAALAQTKLGMTG
                     SFGDLAASSFGGLIAYRSLDRSWLLGKMAELSLLDLVESDWQSLSISPIQLPQGLDLL
                     VGWTGSAASTDSLVSQMESQKSQIEKEQIHVQFLADSKICVEQLIVACQTNDSASVRQ
                     ALTQNRKLLQDFARGMGLVIETPQLSQLCDLAQTYGAVAKSSGAGGGDCGICLVDSKE
                     QKAAIETAWQEAGIFPLTLNIASRE"
     gene            complement(13945..14970)
                     /gene="mvaD"
                     /locus_tag="U1099_000801"
     CDS             complement(13945..14970)
                     /gene="mvaD"
                     /locus_tag="U1099_000801"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEM4867135.1"
                     /translation="MTKQTGIARAHTNIALIKYWGKRDKELFLPMNSSLSLTLDAFYT
                     DTKVVFDPELTADEFYLNGMLQKEKEILKISRFLDLFCEYIGERAFARVESLNFVPTA
                     AGLASSASAFAALALATATALDLDLSPATLSTLARRGSGSSTRSLFGGFVEWDMGTGS
                     EDSMAHPIDDADWDIGMVVLAVNTGPKKIASREGMDHTVATSPFYTAWVETAKQDLVD
                     IKAAIASRDFEKLGQITEHNGMKMHATTLSANPPFTYWSADSLVAQEAVRQVREATGL
                     SAYMTMDAGPNVKVLCRTSQMDELVAELAKVFPREKIITSKPGPAAYVLSEDEWQTSQ
                     AAFEKGL"
     gene            complement(14963..15898)
                     /gene="mvk"
                     /locus_tag="U1099_000802"
     CDS             complement(14963..15898)
                     /gene="mvk"
                     /locus_tag="U1099_000802"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEM4867136.1"
                     /translation="MSVVGKANGKIILMGEHAVVYGQPAIAMPFSAVEIIAQVTAQGE
                     ALTVACDFYKGLVHKMPKIWESLKHAIRFSLYRIGAPTDPAIHIEISSTIPAERGMGS
                     SAAVAVAVARALFAYYKKELTDSELWDIVQSSEKIAHGNPSGIDAATTSGKSPVFFIK
                     HQPIEPFELKFHAHLVVADTGVTGNTLEAISDVADLLEKKPEAIKLVEELGNLTRQAK
                     EDLATDQAELLGSRMNQAHALLQKLGVSDPSLDKLVSLAQENGALGAKLTGGGRGGCM
                     IALARTAQDAQKLAHILDQAGARQTWIQYLGETND"
BASE COUNT         3999 a         4003 c         3505 g         4502 t
ORIGIN      
        1 acgaactctg agtagtggtc ctgggctttt tgcccagtct catcttagaa ttataaatcc
       61 tcaaaggcat cttttacctt gtcaaaaaat ccttttttat ggggtttgac atcaatattt
      121 ccagctgccg caaattcttt aagagctgct ttttggcggt cattcaaacc tgtcggagtg
      181 acgatattta ctgtgacata ttgatcacca atagctccac cacgaacact tggtgcacct
      241 tttcctttga gtcggaaggt cttaccagtc tgagtacctt caggaatgac catatccaca
      301 tcaccgtgaa ctgttggcac atgaactgta tctccaagtg ctgcttggac aaagttgagg
      361 ttcagtttat agtgaatagt ggttccttca cgttcaaact tgtctgaagg ttggacctga
      421 atgacaacgt agagatctcc gtatggacct ccattgaaac cagcttcacc ttgacctgcc
      481 aagcgaatac gttgaccagt ctcaacacca gctggcacct taaccgttac cgtatgggct
      541 tgtttttcat gaccagttcc atgacaggtt gcacatggat ctttaatttg ttttccgcga
      601 ccatgacaga catcacaggt catttgacga cgcatggttc caagtggagt ttgggtgtcc
      661 acattgatca ctccagaacc atgacaacgc ccacaggtta ccggacttgt tccaggtttg
      721 gcaccggaac ctgtacaggt tcgacaagtt gcctcacggt tataggaaac ttcacgttcc
      781 acaccaaaaa tcgcttcttc aaatttcaaa ttgaccgcat attgcaagtc atccccttga
      841 cgaggtgcat taggattgcg cgtcgcacca cctccaccaa agaagctaga gaaaatgtct
      901 tcaaagccac cgaaacctgc tccgtcgaaa ccaccaaagc cacctgctcc tccgccgaag
      961 cctccattgg caccagcagg accatactgg tcataggcag aacgtttttg agggtcgctc
     1021 agagtctcat aagcctcctg gacctccttg tatttatcct ccgcacccgg atccttatta
     1081 atatctggat ggtatttttt cgaaagcttc cgataagcct tcttaatctc gtctggcgaa
     1141 gcattcttag aaacccccag acgatcgtaa aattcagtat tgttcatata agatactaag
     1201 ggcgtaaagc ggacacagcc aaaataggag ttttgacgca ggacctcacc gtcctaggaa
     1261 aaactatctt tttggcacag tccgtagccc gtgttcgatt acgaacactt tcctttctat
     1321 catagttttg gaaaagtgaa aataggaaac tgacgcagtg tgtaaaacac actaggaggt
     1381 ttatcttttt cactaggttt ttagcctgtg ttcgaataaa cgaacttgta ttcctttctt
     1441 tgaaaattag aaagtagccc gtgttcagtt acaaacactt tcctttctgt aaaagttaga
     1501 agggtattta cccaaaaaca gaggctgggt tgcaacctct gtgattggaa attttattga
     1561 cgaatgattt ctattctcag gaagataaga attgagttcg agctaagaac cttaaccgac
     1621 ttcctactaa ttttcaagaa gtcaggataa aaaggtctcc cagtccaacc gctcgtttct
     1681 catttctggc tcacgggcta aaatagtcca caggactatt ttacttttcc gtaaactcgc
     1741 cgtctacaac atcatcgcct gcgttgttag ctgtttgggc accttcttgg cctgcggctg
     1801 cttgttgtgc tgctgcggct tgctcgtaga gtttcactgc aagggcttgg gctttttcgt
     1861 tgaggttttc aagtttagcc ttcatgtcgt ccaagttgtt agcttcttgg gctgctttca
     1921 actcatcaag agcagcttgg gcttggtcac gttctgcgtc gaaacctttt ccttcagttt
     1981 ctttaagagt tttctcagtc gcaaagatgg cttggtcaac atcgttacga aggtccactt
     2041 cttccttacg tttcttatct gcttcagcgt ttgcttctgc atctttcatc atgcggtcga
     2101 tttcttcatc tgtcaaacct gagtttgatt ggataacgat agtttgctct ttttgcgtac
     2161 caaggtcttt agcttttaca gaaacgatac cgttcttgtc gatgtcgaat gttacttcga
     2221 tttgtggaat accacgaggt gctgcaggga tgtcagtcaa ttggaagcga ccaagagtct
     2281 tgttgtctgc ggccattgga cgctcacctt gaagcacgtg gatatcaaca gctggctggt
     2341 tgtctgccgc agttgagaag acttgtgatt tagaagttgg gatagttgtg ttacggtcaa
     2401 tgagttttgt aaagacgcca cccattgttt cgataccaag tgacaatgga gttacgtcaa
     2461 ggaggacaac gtctttgaca tcaccagtga tgacaccacc ttggattgct gcacccatgg
     2521 caaccacttc gtcagggtta acagatttgt ttggctcttt accagtttca gccttaacag
     2581 cttccacaac ggctgggata cgagttgaac caccgacaag gataacttcg tcgatttctg
     2641 acaagctaag tcctgcatct gaaagagctt gacgaacagg tacttttgta cgttctacaa
     2701 ggtcataagt caattcgtca aattttgcac gagtcaatgt catttccaag tgaagcgggc
     2761 ctgccgcacc ggcagtgata aacggcaagc tgatttgagt tgatgtcacg cctgacaagt
     2821 ctttcttagc tttttcagcc gcatctttca aacgttgaag ggccatcttg tcaactgaca
     2881 agtcgatacc gttttctttc ttgaattctg ctaccatgtg gtcgataatc ttttggtcaa
     2941 agtcgtcacc accgagcttg ttgtcacctg ctgttgcaag tacgtcaaag acaccgtcac
     3001 caagttcaag gatagatacg tcgaaagtac caccaccaag gtcgaatacc aagattttct
     3061 cgtctttgtc agtcttgtcc aaaccgtaag caagagctgc tgcagttggt tcattgacga
     3121 tacgttctac ttcaagacca gcgattttac cagcgtcttt ggttgcttga cgttgtgcat
     3181 cgttgaagta agcaggaaca gtaataacgg ccttggttac tttttcacca aggtattctt
     3241 cagcgtagcc tttcaaatat tgaagaatca ttgctgagat ttcttgtggt gtgtattctt
     3301 tgccatttgc tgaaactttt tcagaagttc ccatttttga tttgatagag atgatagtat
     3361 ctgggttagt tactgcttgg cgttttgccg catcaccaac gatgatttca ccatttttga
     3421 aagacacaac agacggagtt gtgcggttcc cttctgggtt tgcgataatt tttgattcag
     3481 ttccttcaag aactgcaact gctgagttag ttgtacctaa gtcaataccg ataattttag
     3541 acatgtgtgt tacctctttt taatttactt tcttttcaag tagtttaacg acatctcggt
     3601 caggattctt tttttgggga atagcagagc ggctactctg atacgactac catagccggt
     3661 ctcagcaagc gttcatgcag cttgtagcct ttttggaaga cttgtgcaat gtgttctgct
     3721 gggcagtcgt ctgtggctgg aactgtttga atggccatgt gaagatttgg gtcaaagaca
     3781 tcggttgcga cttcctccac cccttcttct ttgagggctt gaatcaagct ttcctgtacc
     3841 atttccaagc cttttttgac atcctctgtc aagccttcga cttgaagggc acgttccaag
     3901 ttatccaaac ttggcaagat tttcttggcc aagtcttgcg aacggtagcg ttgaatagtt
     3961 tggcgttcct cgttggcacg gcgttggata ttttgcattt cagcgtgggc acgaaggtac
     4021 ttgttttcaa attcctccgc acgctcattt gccaaatcca attccgattt ttcaggtgtc
     4081 tccacaactt cttctgttgc ttcaacctct tctacgattt cttcgttttt gatttcttct
     4141 gacaagttgt tcacctcttc attttctatt attactttga acaataataa attgagttcg
     4201 ggctagaaac tcagaaaagt gaaatagtcc agtggactgt ttcagcctat ttttcaatcg
     4261 tttccttcac gccctcacat cttaatttac ctcataatga ttactgctca aataacggta
     4321 gtaatcggtt aatttcataa ataggacgcg gctgatgata ttgatcaaac tcatctgcct
     4381 gcggtagttc atgtcaactg gaccgagcaa actcatctgg cccatgcccc gataggggat
     4441 tggaaaacga tgatgaatga ccgtcacatc tgcaagggcc gagtcgctgt gttctgccac
     4501 ggaaatgctg gtctgctggt cgggagccaa gccctgccgc aactctggtg ctaatagctg
     4561 tgcgctatcc aagagttggt aggtagctag attgccgtaa gtcagcgagg caacttttcc
     4621 actgataaag accgattctt gaaaaagatt ggaaaagatg tagtccatca ggtccaagac
     4681 attgtccgtc gtggcaaagt aacgctgcac cacttgggga atctccgtcc gcagcttata
     4741 gtggatggat aagaccgtct ggtcaacgaa gcgttcatcc accaggcgtt tgagcacttc
     4801 caagtccctg gtcaaaaagt ttttaggaat ggcaaactgg acggtgactg gtttggactg
     4861 gtccagagtc aagactgcca gagcatcgtg gctactgagc tgcacgatgt caaaggaagt
     4921 caactgctga ctggtcggct ccacatctaa gatgaccgaa gtgtagccag tcaaatccgc
     4981 taggactgta ctggcccgct ccaagatgtc ctccagcttg aaggcttcaa agtcgaaggc
     5041 cttgaccacc tgataaacat cttcttcatc gatgtgttcc agattgagcg agtggttgac
     5101 aaagtattga aaaccagccc gactgggcat ccgtccactt gatgtgtggg ccttttccag
     5161 caaacccaac tgctctagct tggccatgtc attgcgaatg gtagccgaac ttgaagcaat
     5221 catttcctgc aaggccttag aaccaactgg ctcatgatgg cgcgtaaata attcaacaat
     5281 cagattcaaa atatcatttt gacgttgggt aatcatctcc gctcctttca tgaacacgat
     5341 tgatttttag ctttagcact ctcgtatgac gagtgctaaa ctatatactc ccattatacg
     5401 ccgaaaaaat ggattgtcaa gagaaaaaga caaaaaatta gcactctttt acctagagtg
     5461 ctaaaaatca gtctgaggac ctagttactt ccctacttca atcaaaaaaa tcagagaata
     5521 tccctgattt catatcttta tttcccttgg gcaatcaatt ctgctccacg ttttcggtag
     5581 gataggtcgc caactgcttc aattaaaagc ttgccatttt cgtatttgag gacggtaatt
     5641 gagccgttat cgagaaagat attagcacca cgctctggct ctaacagatg agccagggtg
     5701 gcaatggtca tgccgtgact aaccacaaga gcattgccac cgccctgttt tttcaaatct
     5761 tgggcaatag cctcaaaacc tgtcaagata cggttactca agacctccca cgattctgcc
     5821 cagccagcgg tgtctgcttc ttgaatacct gctgcgattt ccgcaaaagt catgcctgtc
     5881 gcatcaactg tccctttcaa acgaggcagg acaccttgga aaagctcggc atcgtacatg
     5941 ccttcaaaac taccgaaaca ccattcacgg atacgcttgt cttggtagta agggattttt
     6001 cccaaaatcc ccaactcacg ctgggcgatg gtcatggtct gaacggttcg acccaggtca
     6061 ctggacacgg ctagtttaaa ttcaaggccc gcatccttga gccccaagcc caattcacga
     6121 atcccttcct caccagcctt ggtcagcggc gtatcacacc agccctgcac gcgcccaatg
     6181 gtattaaaca tggttttccc gtgacgagaa atatataatc ttacatctgc cattcgtttt
     6241 tctccttgtc atttcttact attagtataa caaaaaaccg actgcttgtg gcaatcggtc
     6301 tacgtttttt agttcttaaa gctgtgaatc ggtgctggaa tttgtccgcc acggttgata
     6361 aagtccgcag aagaagcctg attgaccttc atgactggcg ctgttcccag aaggccgcca
     6421 aattcgatca tgtccccttc ttttccaagc ggaataatgc ggaccgcagt cgttttctgg
     6481 ttgataaccc caatggccgc ttcatccgca atcatagctg caatggtttc agcaggtgtt
     6541 gtttctggaa tagcaatcat gtccaaacca acagaacaga tagccgtcat agcttctaat
     6601 ttttcaaggt taagggagcc attttgcacc gcagctatca tgccctcgtc ctcagacaca
     6661 gggatgaagg cacctgacaa gccaccgacc tggttacaag ccatgacccc accctttttc
     6721 actgcgtcat tgagcagagc aagggcagca gtcgttccgt gcgtaccaac ggtttctaag
     6781 cccatttcct ccaaaactcg tgccactgag tcgccaaccg ctggcgttgg agcaagggac
     6841 aggtccacga taccgaactt gacacccagg cgttcgctgg ccatatttcc taccaactga
     6901 ccgatacggg taatcttgaa ggcggtcttc ttgacggtct ccgccaccac atcaaagctc
     6961 tcgccacgca ctttttcaag ggcacgcttg accacaccag gtccagacac gccgacatta
     7021 atgaccacat ccgcctcacc gacaccatgg aaggcacccg ccataaaggg attgtcctcg
     7081 accgcattgg caaagaccac caacttggca gcccccatat cagaagcctc cgccgtctcc
     7141 ttgatgatcc gccccatgtc ccgcacagcc gtcatattga tacccgtctt agtcgagccg
     7201 atgttgaccg acgagcagac ttttgtcgtt tgggccaatg cctgcggaat agagttgatg
     7261 aggatttcat ctcctttttg gtagcccttt tgaacgagag ctgaaaagcc cccgataaaa
     7321 tcaattccga tctcatgagc cgccttgtcc aaggcatgag ctaggggaag gtaatccgtg
     7381 gcatccgtcg ccgcaccaat caaggcaata ggcgtcaccg acacccgttt gttaacaaca
     7441 ggaatcccca gctctgccgc aatctcatcg ccgactgcta ccaagttctt ggccttggtg
     7501 acaatcttgg tatacacttt ctccgcagct ttttcaatat ccgcatcaat acagtccaag
     7561 agggaaattc ccatggtgat ggtccgaata tcgaaattct gctcctcaat catttcaatg
     7621 gtttctctaa cctgtctaat atccatgaaa tctctcctta caagttgtgc atagcatcaa
     7681 aaatggctgc actttgaatg ttgattttca cgttcaaacc ttgaccaaag gcttctagtt
     7741 ctgcacgaag cttggtgaaa tctttcttct catccgacga caccaccgcc atcatggtaa
     7801 aatactcatc caaaacagtt tgggaaatat cgtcaatatt gagccctaac tccgcaatct
     7861 tagtcgcaac acccgcaaca attcctgact tgtccttacc gacaactgta acaatagctt
     7921 tcatctgaac acctcttcta aattttcatt tattttatca taaatagaca gaaaaaggta
     7981 aaaatctttt gatttctacc tgtatcgtac gtgtttacaa ctccaactgt ttcttctatt
     8041 cggctacggc tagagttgcc tagtttttaa gttgcaaact ttctatttct accagtctgt
     8101 gatacactag tgtcaccgaa ttctattatt tgttggttgc gacaagctac caacctttct
     8161 tttgttcaga aaggatactt atgaagatta tcaagcaatt gtggtggttc ttttcccttg
     8221 agaaaaaggc ctatctgatt gggattttat cactctgttt ggtcagcctc ctcaatctcc
     8281 tgcccgcctc tatcatgggg cagttgattg accagattgc cagcggacag ttgactgagc
     8341 atcgcttgct actcggggta gctgggctca tcttgtcagc cttggccatg tacggcctcc
     8401 gctatctttg gcgaatgaac atcctggcaa catcttatcg tctaggaaaa atcatgaggg
     8461 ctcggctgtt taaccacttt atgcacctat ccccttcctt ttttcagcaa tatcgaacag
     8521 gggatttaat ggcccatgcg accaatgata tcaatgcctt gacacgacta gcaggtggtg
     8581 gcgtcatgtc ctttgttgat gctaccgtta ctgccttggt tacactggtc accatgagtg
     8641 tgagcatttc ctggcagatg accttggttg caatcctccc cctgcccttc atgacactca
     8701 ctactaattt tctaggccgt cgtacccatg aaaatttcaa ggcttcacaa gcagcctttt
     8761 cagagcttaa caacaaggta caggaagctg tttctggtat aaaggtcact aaatcctttg
     8821 gctaccaaga acaagaaacg gcggcttttc aggaagtcaa ccaagcagcc ttcttgcaga
     8881 atatcaaaac catgcgttac gatgccctgt tcaaccctgc cgtcctcttt tttattggcc
     8941 tgtcctacct gctgaccctt ctggtcggct ctcacttcat ctcgcaaggt caggtcagtc
     9001 tgggccagct agtaactttc atgacctatt tagatctcct cgtctggccg ctcatggcca
     9061 tcggtttttt ggtcaatatt agccagcgtg gtgatgtatc ttacaatcgg attcagaccc
     9121 ttctcagcat ttcctcagag gtcattgaaa cagaccaacc gctgcctgca ccaagcaatg
     9181 gagagattgt ctatgagatt agcgaatttg cttatgataa cataccagtc ctccaagaca
     9241 tccagtttca catcaaaaaa gggcagacaa ttggtctggt ggggccaaca ggctctggga
     9301 agaccagcct actcaagctc ctcatgcgag aatacgatgt gacaaatgga cagattttgc
     9361 tcaatcagca aaacataaag cactacaagc tcggtgacct acggcagttg atgggctatg
     9421 ttccccaaga ccagttcctc tttgcaacca gcgtggctga gaatatccgc tttggcaatc
     9481 cagacctctc actagaccag gtgaaagagg ctgccaaagc tgtccatgtc tatgaggata
     9541 tccaggatat gccagatcgc tttgacacca tggttggtga aaaggggatt tccctgtctg
     9601 gtggacaaaa gcaacggttg gctatggctc gtgctatgat tctcaatcca gatattctac
     9661 tcttggacga ttcgctctcg gcagtggatg ccaagacaga gcacgctatt cttgaaacca
     9721 tcaagcaaga acgccttgat aagaccacca ttatcaccgc ccatcgtctg tcagctattg
     9781 tccatgccga tttgattctg gtccttgaag atgggaaaat cgtagaacga ggtcgtcacc
     9841 aagaattact agatgaaaaa ggctggtact acgataccta tatgatgcag caactcgaaa
     9901 aggaggaaag tgatgctctc taaaaccaag caagccagtg ttttttggcg actcttgtcc
     9961 tatctcaaag cctatcgact gctgacagtc cttgccttga tctttttatt gctgactacc
    10021 gtagtccgca gtctaattcc ccttctagcc tcttacttta tagaccacta tatttcctcg
    10081 atttctgaga cagctgcggc tattctagcg gcctactttg ccctctatct gctccaaatg
    10141 gtgcttcaat attttggaaa tctctggttt gcaaaagttt cttacagcat tgtcagagat
    10201 attcgccggg atgcttttgg gagaatggaa aagctgggaa tggcttactt tgaccagact
    10261 cctagtggct ccattgtctc gcgaatcacc aatgatacag agagcatttc agagatgttt
    10321 tcagggattt tatccagttt tatctcagct ctctttatta tcatcacgac tctttcgacc
    10381 atgtttgctc tggattggaa attgacctgt ctgatcgtcc tctttcttcc cttgattttc
    10441 ttattggttg atcaatatcg gaagcggtca gctcccattg tggccaagac tagaagtctg
    10501 ttgagtgcca tcaacagtaa actggcagag agcatcgagg ggattcggat tatccaggcc
    10561 ttttctcaag aggagaggct aaaggaagag tttgaggcta tcaaccagga acatctggac
    10621 tatgccagtc gttctatggc cttggacagt ctctttctcc gacctgccct gtcacttgtt
    10681 aagattttgg cctatgccct actcatgacc tattttggtt tggactggtc acaggctgga
    10741 atttctgctg gtcttatcta tgctttcatc cagtatgtca atcgtctttt cgatcccttg
    10801 ctggatgtca cgcagcattt ttccaccctt caaacctcta tggtatctgc cggacgggtc
    10861 tttacgctca tggaccagac ctttgaggaa ccgctacagc tggaccgagg ggccaagatt
    10921 gacaaggggc acattgtctt tgaggatgtt tgcttttcct atgacggcaa gcggcaaatt
    10981 ctggacaagg tttcctttga agtgagacag gggcagacca tcgcctttgt aggtgctaca
    11041 gggtcaggca agtcttctat catcaatgtc tttatgcgtt tctatgagtt ccaatctgga
    11101 cggattttga ttgacggtca ggatattcgt agcttttcgc aggaagaatt gcggtccgcg
    11161 atcggcctag tcctgcaaga tccctttcta tttcatagga ccattgcatc caatatccag
    11221 atgtatcagg atatttcccg agaggagatt gaggaagcgg ctcgatttgt ggatgctgct
    11281 ccctttattg agaaacttcc tgagaaatat gaccacctgg ttactgagcg gggtaccgcc
    11341 ttttcgacag gtcaacggca acttctggcc tttgcacgca ccatggccag tcagcctaag
    11401 attcttattt tagacgaggc taccgccaat atcgattcgg agacagagca aacggtccag
    11461 acttctcttg agaaaatgcg aaaagggcgg acaaccattg ccatcgccca ccgcctatcg
    11521 accattcagg atgccgactg tatttatgtc ttggataagg gaaagatcat cgagtcaggc
    11581 aatcacgaag aacttctagc cctcaagggt cactaccacc gtatgtacca gttacaagca
    11641 ggccagttaa cagcaaccta gaaaccactc catgacaaaa gccaagttcc aaaatggacc
    11701 tggctttttt atttttctac attgttgaga actgaagaca tcaatctatc tctctttaac
    11761 tcacattcgc tgaccgacgg gcataggctt tccagtcaat cccacgcgct tcacaccagt
    11821 tttgtatatt tggagccaag accaaatctt tctgccggag ttccgcaata ttcttagcac
    11881 ccagcatggt catgatttca gccatctgcc attgataagt cttaatagcc tgcaagccgt
    11941 cgtcaaaacg atgcccctta ccatccttga catactgaag gaaatggttg gacataccaa
    12001 caaggtctgc accgagggcc aatgatttga caatatctag tggcgtctta ataccaccag
    12061 atgcaatcag gcttgggcga acttcttcag aaacagacat ggcttctacc aaggaagtca
    12121 cggtagactg cccccaacct tctagataag tataatcatt gaaggtacgg cgggcatttt
    12181 caatcttggc gaaatccgtc cccccagtac cagacacatc aatagtttgc acaccgacag
    12241 aagccaactg ggcgacagtt tcacggctca tgccaaaacc aacttccttg acgataacag
    12301 gtacttccat ctcacgtaca agggtttcaa tatttttcaa ccacatagta aagtcacggt
    12361 ctccctcagg catgacaatc tcctgggggg cattgacatg gatctgaatg gcatttgcct
    12421 ccaacaaatc caccgcccgc ttggcattct caacactgtg atgggcacct aggttggcaa
    12481 agataatccc gtaaggattt tcccgacgca taacagagaa ggtttccgca acagatggat
    12541 ccttgatcgc cgcactgacc gaaccagaag ccagagcaat cttcccaaaa tgtcccataa
    12601 ttcccaacag acggttgatt tcccgcgtct tcttgcttcc accggtcatg gcattgatga
    12661 agaaaggaaa ggcaaagtcc aaaccagcta cgctggtcga aatatccact tcatccactt
    12721 tggtttgagg cagggaatga tggacaaaca aggtctccgt aaagtccttg gccggtgtgg
    12781 cactatattg ctggttggcc aagccaacgt gttgatcctt gcggtctaaa ccaaattctc
    12841 ctagataaaa catagggttc tccttattct ctacttgcga tgtttagggt gagtggaaaa
    12901 ataccggctt cttgccaagc cgtctctatc gctgcttttt gttccttact gtctaccaag
    12961 caaatgccgc agtcaccacc acctgcacca gatgacttgg ccaccgcccc ataggtctgg
    13021 gccaggtcac agagttgact cagttgaggc gtttcaatga ccaaacccat tcctctcgca
    13081 aagtcttgta agagcttgcg gttctgagta agggcctgcc ttactgacgc tgagtcattt
    13141 gtctgacaag ctactatcaa ctgctctaca cagatcttag agtcggctag aaactggaca
    13201 tgaatttgct ctttttctat ctgacttttt tggctttcca tctgggaaac tagactatcc
    13261 gttgaagccg ccgaccctgt ccagccaacc aagaggtcta agccttgggg taactggata
    13321 gggctgatag acaaactttg ccaatcactt tccaccagat ccagcaggga cagctctgcc
    13381 attttcccta aaagccaaga acggtccaga gaacgatagg ctatcaagcc accaaaactg
    13441 gaagccgcca aatctccgaa ggaacctgtc atgcctagct tggtctgggc caaggctgcc
    13501 agcttgtagg tcaggagggc atctggtcgg tgaccgtagt aggtcaacag ggccttgacc
    13561 gtcgcaaccg ttactgcccc tgacgacccc agaccatact tggcacctga agtctggtca
    13621 tccaggtcag actggaccgc tagactgtaa gttccatgac aagcatagcc cttggctgtc
    13681 agataggctt ctgtcacctg catgactgtc tcaatcagag catagggatg gtcaccttgg
    13741 acatgagcga taccttcctt acgctcccaa atcagataga ggtctacttg ctggctggaa
    13801 tgaagaaggc ctcggtcact tgtttcaatg gtgacggtca ggtactggtc aaccgctgca
    13861 atcaccgcag gataaccggc ctcgacgact gcgtattccc ctgctagaaa gagttttcca
    13921 ggtatcttta cttgtacttt catgctacaa gcccttttca aacgctgctt gtgaggtctg
    13981 ccactcgtct tcactgagga cgtaggcagc aggaccaggc ttgctggtaa tgattttctc
    14041 tcttgggaag actttggcca attcagctac tagctcatcc atttgacttg ttcggcagag
    14101 gaccttgaca tttggtcccg catccatggt catgtaggct gataagccag ttgcttcacg
    14161 aacctgacgg actgcctctt gagccactag gctatcggct gaccaatagg taaagggtgg
    14221 attggcagag agcgttgttg catgcatttt catgccattg tgctctgtaa tctgaccaag
    14281 tttttcaaaa tcacggctgg caatagctgc cttgatgtcc accaagtctt gcttagcggt
    14341 ttctacccag gctgtgtaaa atggtgaggt ggcaactgtg tggtccatgc cctctcgact
    14401 agcaatcttt ttcggtccgg tattgaccgc caagacgacc atgccgatat cccaatccgc
    14461 atcgtcaatg gggtgagcca tagaatcttc cgaaccagtt cccatatccc actcaacaaa
    14521 tccaccgaat aggctacggg tactagagcc tgagccccta cgagcaaggg ttgatagggt
    14581 ggctggtgac aaatctaaat ccaaagcagt tgcggttgca agtgcaagtg ctgcgaaagc
    14641 tgatgctgag cttgccaaac cagccgcagt cggaacaaaa tttagacttt ccactcgggc
    14701 aaatgccctt tcacctatat attcgcaaaa caaatccaaa aatcgagaaa tttttaaaat
    14761 ttctttttct ttttgtaaca ttccgttgag atagaactca tcggcagtta attctgggtc
    14821 aaaaacgacc ttggtatccg tataaaatgc gtcaagggtc aaggataggc tagaattcat
    14881 aggtagaaat aattccttat ctcgttttcc ccagtattta atcaaggcaa tattggtatg
    14941 ggcacgggct atgcctgttt gtttagtcat tggtttctcc taagtactgt atccaggttt
    15001 ggcgggcgcc tgcttggtca aggatgtgtg caagtttttg ggcatcctga gctgttcttg
    15061 ccagggcaat catgcagcct cctcggccgc caccagttaa tttggctccg agggctccat
    15121 tttcttgggc aaggctgacg agtttatcta ggctaggatc agacacgcct aatttttgca
    15181 ggagggcatg ggcttggttc attctgctgc ctagaagctc tgcctggtct gtggctaaat
    15241 cttccttggc ctgtcgggtc aagttaccca gttcttctac tagcttgata gcctctggct
    15301 ttttctccaa caaatcagcc acgtctgaaa tggcttctaa ggtgtttccc gtcacacctg
    15361 tgtcagccac taccaaatgg gcatggaatt tcagctcaaa aggttcgatg ggctggtgct
    15421 tgataaaaaa gacgggagac ttgccgctgg tggtcgctgc atctatgcct gatggattgc
    15481 cgtgggcaat tttttctgaa gactggacga tgtcccagag ttcgctgtcg gtcagttctt
    15541 ttttatagta ggcaaagagg gcgcgtgcta ctgcaactgc cacggctgca ctggagccca
    15601 taccgcgctc ggcaggaatg gtggaactaa tctcgatatg gatagccggg tctgtcggtg
    15661 ctccgatacg atagagcgag aaacggatgg catgcttgag gctttcccag atttttggca
    15721 tcttgtgcac caagccttta tagaagtcac aagcaacagt cagcgcttcc ccttgagcag
    15781 ttacctgagc aataatttca acagcagaaa aaggcatagc aattgctggt tgcccataga
    15841 ccaccgcatg ctctcccatc aaaatgattt ttccatttgc ttttcctact accgacatac
    15901 ttttctcctt atacaatcac tactattata ccatttttac ataagaatag aacacacgaa
    15961 aacagccgac cgcggtcgac tgttctctag gagattatat gaaaaagaa
//
LOCUS       DASFSW010000032        15994 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.032, whole genome shotgun sequence.
ACCESSION   DASFSW010000032 DASFSW010000000
VERSION     DASFSW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15994
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.032"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(30..1052)
                     /locus_tag="U1099_000803"
     CDS             complement(30..1052)
                     /locus_tag="U1099_000803"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEM4867137.1"
                     /translation="MTVNIDWEKLGFSYMKLPYRFIAHFKNGQWSEGQLTEDAELHIS
                     ESSPALHYGQQAFEGLKAYRTKEGKLQLFRPDQNAERLQRTADRLLMEPVPTDLFIKA
                     CKEVVKANADYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTVFAMPVGNYFKGG
                     LAPTNFLIQDKYDRAAPNGTGAAKVGGNYAASLLPGQYAKKQGFSDVIYLDPATHTKI
                     EEVGSANFFGITADNEFVTPISPSILPSITKYSLLYLAEHRLGMKAVEREVLVEELDQ
                     FVEAGACGTAAVISPIGGIQIADKLHVLHSETEVGPVTRKLYEELTGIQFGDIEAPEG
                     WIVEVD"
     gene            complement(1163..1780)
                     /locus_tag="U1099_000804"
     CDS             complement(1163..1780)
                     /locus_tag="U1099_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEM4867138.1"
                     /translation="MKIIKLFFSIKVYLWLVLSFLLLLCGVWGYGYISGEKSNQQEVS
                     IHTAIESVEQVNELVFLNTAVQKIVTAKNYTVLFGQEMPFSEKSALLIIKYKAKFGIK
                     SPVSIEHLSENRYKITLPAFKVIGLELDAEEPYTLYDKSGEILSFATEEIDTAQLITD
                     EFQSAEQETFLADYQDDIKESAKTYYQNLFSAISPEIQLEFVFKE"
     gene            complement(1952..4405)
                     /gene="parC"
                     /locus_tag="U1099_000805"
     CDS             complement(1952..4405)
                     /gene="parC"
                     /locus_tag="U1099_000805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEM4867139.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFDKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEMAGYLLADIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPTAKLEKLMEFLPGPDFPTGAI
                     IQGADEIKKAYETGKGRVVVRSRCEIEQLKAGKKQIVITEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIAEVRDESDRTGLRIAIELKKDSDEQTILNYLYKYTDLQINYNFNMVAIDN
                     FTPRQVGLQKILSSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLENEEAALREQIQTLA
                     AIIGDERTMFNLMKKELREVKKQFGNPRLSELQDQAETIEIDTASLIVEEETFVSVTK
                     AGYIKRTSPRSFNASTLEEMGKRDDDQLIFLQNAKTTQHLLLFTNLGNVIYRPVHELT
                     DIRWKDIGEHLSQTLMNFDTSEEIIFAELVENFEEGTYFAVTQYGQIKRVERKEFTPW
                     RTYKSKSTKYAKLKDAEDVVITVSPVVLDDIMLMTEKGYALRFNIEEVPIIGAKAAGV
                     KAVNLKDGDVVVAAFISNTSSVYLLTQRGSFKRMATEEIPVTSRAKRGLQVLRELKAK
                     PHRVFAAGPVLTDQGDFDLFSTANEDETTSQILHVQSKTGKLYEVDVTQLSLSERTSN
                     GSFISDTISDEEVFRAWID"
     gene            complement(4606..5058)
                     /locus_tag="U1099_000806"
     CDS             complement(4606..5058)
                     /locus_tag="U1099_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867140.1"
                     /translation="MIKFLRKKLTIEQLKKVPYASQYTEVLRSIWRADVPKYGISSTL
                     QGELLRQLEKLRWEAQANGNVNWCEEHSNYCRFIKETLYKGKVLSSQQKQELVLIMDY
                     LKSCGEYAQAYQENLIDDEELEIEKLAYVDDNLYDRVGDMIAFFYQRT"
     gene            complement(5061..5555)
                     /locus_tag="U1099_000807"
     CDS             complement(5061..5555)
                     /locus_tag="U1099_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEM4867141.1"
                     /translation="MPKLTDRFQKRLSEWARKRKPFKNKQELEEKFDQALKIWQSSKT
                     RQHAENLLSEQGKLEHFLHSTERKLSRMPFGGDKFAAIPGLISMVRSYIRRDYSKLPK
                     GTILAIIGALIYFFSPIDALPDFILGAGLLDDAFVLNACLKLIKTDVDDFRSWQASQW
                     KVDE"
     gene            complement(5588..6190)
                     /locus_tag="U1099_000808"
     CDS             complement(5588..6190)
                     /locus_tag="U1099_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEM4867142.1"
                     /translation="MVLENKLGIENSAELARLEEQISKKKAAQLFETGQLFQIEVGTF
                     AGLAHIHQTLFEEIYDFSGKIRDVNIAKDNFQFAPRIFLEQSLAYIDKLPHETFDEIV
                     EKYADMNIAHPFREGNGRSMRIWLDCMLRDSLGKVVDWNSIDKDEYFNAMVRSHVSTG
                     ELKYLLLQALTEDLGQATYFKGIDHSYYYEGYNLYRTEDL"
     gene            complement(6214..8163)
                     /gene="parE"
                     /locus_tag="U1099_000809"
     CDS             complement(6214..8163)
                     /gene="parE"
                     /locus_tag="U1099_000809"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEM4867143.1"
                     /translation="MAKKEIDINNYNDDAIQVLEGLDAVRKRPGMYIGSTDGNGLHHM
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLSVSDRGRGMPVGMHATGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFEQGGKPVTTLE
                     KIGTAPKSKTGTKVTFMPDDTIFSTTDFKFNTIAERLKESAFLLKQVTMTLTDERTGE
                     QEEYHYENGVQDFVSYLNEDKETLTPVLYFEGEDAGFQVQVAMQYNDGYSDNILSFVN
                     NVRTKDGGTHETGLKLAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVSDKLTFFLLENGELASNLVRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGSDFTLEDVNYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGRVYIALPPLYKMSKGKGKTEKIAYAWSDGELED
                     LRKDFGKGFILQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVSVLMGD
                     KVEPRRKWIEDNVKFTLEEATAFTK"
     gene            8306..8950
                     /gene="plsY"
                     /locus_tag="U1099_000810"
     CDS             8306..8950
                     /gene="plsY"
                     /locus_tag="U1099_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEM4867144.1"
                     /translation="MLVNLILLFIAYLLGSIPSGLWIGQTFFNINIREHGSGNTGTTN
                     TFRILGPKAGTIVFVIDFLKGTLAALLPLIFHAQGISPIVFGLMAVLGHTFPIFAQFK
                     GGKAVATSAGMLPGVAPAFCLYLVIIFVTSLYLTSMVSFSSVLAAALAILGALLFPAI
                     GIILPSYDWLFTVIIVFLGSFVIIRHKENIQRILKKEENLVPFGLNLTKQKKRA"
     gene            complement(8960..12613)
                     /gene="addA"
                     /locus_tag="U1099_000811"
     CDS             complement(8960..12613)
                     /gene="addA"
                     /locus_tag="U1099_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEM4867145.1"
                     /translation="MAFEQFLSAEEIKAVQLAEANSDKQQKRTPEQIEAIYTHGQNVL
                     VSASAGSGKTFVMVQRILDKLKRGVGIDQLFISTFTVKAAGELKERIEKNLNETIAET
                     MDMDLRRHLSAQLADLTKADIGTMDSFTQKLVTTYGYSLGISPQFRILQDETEKASLK
                     KEVFDQLFADYLEQDENGAFRKLVRNFSGNRKDNGGFRQVVYQVHDFSQSTSSPTKWL
                     KEQAVQADLYSQERIELILEQGFKEKVLDKLYQAADFFRYHVEWGRNDFGSAKYFANV
                     EEVLDLLTGLDSLDQKDLMERVEKVLLINDQSRGKGLTNANRPKDEHLIAFKEEYNAG
                     KSQIISGLRDLGQEVYELTLLKDYQIQALPMLILLRDFVLDFSQAYLDVKIKEAAFEF
                     GDIGHFAIRILEENVDIRQFFQEKYHEVMVDEYQDNNHSQERMLDLLSNGHNRFMVGD
                     IKQSIYRFRQADPMIFQEKFELYQADSQAGKLILLKENFRSQIEVLEATNAIFTRLMD
                     RQVGEIKYDETHSLVAGSPGQKIAQPKHEMEYLIYDQQDSANSSTDAEEETPLTAGEI
                     EVVAKEIIRLHNEEGADFKDITLLVQKRTHNDLIISIFEKHGIPIVADGGAASYLQSL
                     EVMIMLDTLRVINNPLNDYALVALLKSPMFRFDEDELTRISLQAGTGFFYQKMEIAQQ
                     TNGQHPELMSGELKKKIKDFLSILENWRAFAKMHSIYDLIWKIFNEKFYYDYVGALPN
                     GSKRQANLYALGLRANQFEKTGYKGLSRFIVMIDRALANDKDLADVQEFLPQNAVQLM
                     TIHKSKGLEFKYVFLMNIDKRFNLEDHYQSVIISRKNGLGIQYLADMKDKVNSPLPQV
                     RVLMNTLPYQDNLQELKIANLSEQMRLLYVALTRAEKKLYLVGKGNADKLAKKYDGKK
                     ADGVLAQSTRESVATFQDWILAIDEAFSGEDLHFKKVFVTDEDLTEEKIGKLTLKSKL
                     EDASLKDIRQSEDIAQALDQLSSVQELNERYKVAIELPSLRTPSQIKKLYEPILEQEG
                     MEVMEKYQPKRTFNLPDFSKKPKITGAQVGSAVHELMQRLDLSWLVTEDTVREALEAV
                     HAEQAIKDKINVQMILDFFDTDLGREILANTDKLHREAPFASLQTDSVSQENFVLRGI
                     IDGYLLYDDHIVLFDYKTDKYDQPSQLSQRYQAQMQLYAEALKKAYKIDRVDCHLILL
                     GGERIEVVEVKVN"
     gene            complement(12613..15879)
                     /gene="rexB"
                     /locus_tag="U1099_000812"
     CDS             complement(12613..15879)
                     /gene="rexB"
                     /locus_tag="U1099_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEM4867146.1"
                     /translation="MKLVYTDIRNPLTQYLTDTSAEFAKEAKRVFYIAPNSLSFEMER
                     KVLEYLPEQATFDIIVTRFGQLARYLMIDRKEAGQPLDDVGLAMIFFRVLSQFEDGDL
                     KVYGRLQRDFGFINQLVALYKELQRANMSILDLEAMDSPDKQADLVKIFLAVTDILSK
                     ESFEHQSKLAQLTGLVETGQLDEQLKNIVLVVDGFSRFSAEEEALVSALNERVSEILI
                     GVYASKKAVQVIYAEGNVYQANVDFLRQLSAQFQTKATYIGQEPVLDSIGKFSKNMEA
                     YYDYSGTMIDLTPADQEKIQLWEVVNQKEEVEQVATAIRQHIHQGARYKDILLLLGDV
                     DSYKLQIGKIFDKYDIPYYFGKAEEMSHHPLVHFVESLERLRRYRFRAEDLLNLLKSG
                     LYASISQKELDLFESYILFADMKGQAAFSRAFSVNGRADYDAEVVKEKRLVYDLAVLE
                     PLRAKIMEPLNQLFKAGPQSGATLLEKFMAFLEAIEFPKNMEKMSRNLSEVEQEKEEQ
                     VWKSFTHLLENFHQIFGKEKLKMDDFLAILQAGMQASHYRTVPATVDVVNVKSYDLIE
                     PHTAKYVYAIGMGQSNFPKVAKNTSLLTEEEMEKVNLVSASSSRFDLVSRENIKKNHA
                     AMMSLLNAATEQLVISTPQIYNEGEDSLSPYVKILKEMGLSSEERGRIKSLSPQDIGH
                     YKSLLSRLIESERPSLDNEEWEGQRAFWTVLVRHLKKKLESQGIEIPTITGDITSKQL
                     AADTLAALYPEDKPLNLSASSLTNFYNNQYLYFVRNVLRLREQESIHPTAFQHGLFLH
                     RIFERVVMDQSELDFDQKVDKAILRTRDEAEFAMFYNQDADARYTEEVLDKIARSSAT
                     ILRDNDLVEIDGQEKSFRQDKALVFDLQNGKSVHVNGTIDRLDTLQINQAVGVVDYKS
                     SDQSFSVGDFYNGLKPQLVTYLAALQELDETKDKPVFGAMYLHLQDPIIKLKDTKNLE
                     QLEGAANTSLVYKGLFLKEESLGLNHFYQTRNQLYTEDEFAVLLNHNQELYKQAAMDI
                     LAGRFAINPYTKDGRSVAGEQLKAITGFEADRHMGMARRLVKEAKRQDWMERMKGGQD
                     "
BASE COUNT         4291 a         3756 c         3218 g         4729 t
ORIGIN      
        1 aagagcttgc aagtgcaagc cttctgaaat taatctactt cgacaatcca gccttcagga
       61 gcttcaatat caccaaactg gatacctgtt aattcttcat acaatttacg agtgacaggt
      121 cctacttctg tttcactatg gagaacatgg agtttgtctg caatctggat accaccaatt
      181 ggtgagatga ctgcagctgt tccgcaagcc cctgcttcga caaactggtc taattcttcg
      241 acaaggactt cacgctccac agccttcata cccagacgat gctctgccaa atacaagaga
      301 gaatacttgg taatggacgg caagattgat ggtgaaatcg gtgtgacaaa ctcgttatca
      361 gctgtgatac caaagaaatt agcagaccct acttcttcaa tcttggtgtg ggtagctggg
      421 tccaggtaga tgacatctga gaagccctgc tttttggcat attggcccgg taagagcgat
      481 gccgcgtagt taccaccaac cttagctgcc cctgttccat ttggtgccgc acggtcgtac
      541 ttgtcctgaa tgaggaagtt ggtcggagcc aaaccaccct tgaagtagtt gccaactggc
      601 atggcaaaaa ctgtgaaaat gtactcttct gctggtttta cgccgatgat gtcgccgacg
      661 ccaatcagga gcggacggag ataaagggtg ccacctgttc catagggagg aacgtagtct
      721 gcgttggctt tgaccacctc tttacaagcc ttgatgaaaa ggtcagtcgg tacaggctcc
      781 atgagcaagc ggtcagctgt ccgttgtagg cgctcagcgt tttggtctgg acggaaaagt
      841 tggagctttc cctccttggt gcgataggct ttaaggccct cgaaggcctg ctggccatag
      901 tgaagggctg gggagctttc tgaaatgtga agttctgcat cttcggtcag ctgcccctca
      961 gaccactggc cgtttttgaa atgagcgatg aagcgataag gtagtttcat ataagaaaaa
     1021 ccaagttttt cccagtcaat gttcactgtc atatagtttc tccaaattca ttttttatta
     1081 ttttactact tttgaaagtg attttcaatt aaaaattcag aaaattttgt gaatataaga
     1141 aaaagcctgg taaaccagac tcttactctt taaaaacaaa ttctagttga atttcaggtg
     1201 agatagcact aaataaattt tgatagtagg ttttagctga ctcttttatg tcatcttgat
     1261 aatctgctaa aaacgtttct tgttctgctg actgaaattc atctgtgatg agctgggcag
     1321 tatcgatttc ttctgtcgca aaactcagta tctcaccact cttatcatat aaggtataag
     1381 gttcttctgc atctaattcc aatccaatga ctttaaaggc tggaagggta attttgtacc
     1441 gattttcaga aagatgttca atggatacag gacttttgat gccaaatttt gccttgtact
     1501 taatgattaa gagggctgat ttttctgaga agggcatttc ttgaccaaat aagaccgtgt
     1561 agtttttagc ggtcacaatt ttctggacag ctgtatttag aaataccaat tcattgactt
     1621 gctcgacaga ttcaatagcc gtatggatac tcacttcttg ctgattactc ttttcgccag
     1681 aaatgtagcc gtatccccat acaccacaca ataacagaag aaaggacaga acaagccata
     1741 gatagacttt tatactgaaa aataatttga taattttcat agcatttacc tcctctacag
     1801 tatagcaaaa agactgcttt acaggcctcg attagtacca accagtcagc tggtgaagcc
     1861 agtcctcaaa ggtaggaaag actttcatgg caaggactat cagtactaag atagtgaaca
     1921 aaataaggaa tttctttttc attctaaata cctaatcaat ccaggctctg aagacttctt
     1981 catcggaaat ggtatccgaa atgaagctgc cgttgctagt gcgttcagac aggctaagct
     2041 gggtcacatc aacctcatag agtttgcctg ttttggactg cacgtgaagg atttgacttg
     2101 tcgtttcatc ttcatttgct gtgctaaaga ggtcaaaatc cccctggtcc gtcaagactg
     2161 gacccgccgc aaagacacgg tgaggcttgg ccttgagttc gcgaagcacc tgcaaaccac
     2221 gcttggcacg gctggtcaca gggatttcct ccgtcgccat ccgcttaaag ctaccacgct
     2281 gggtcagaag atagacagag cttgtgttgc tgataaaggc cgcaaccacc acatctccat
     2341 ccttgaggtt gaccgccttg acaccagccg ccttggcacc gatgattggc acttcttcga
     2401 tgttaaatcg cagagcatag cccttttctg tcatgagcat gatgtcatcc aagactacag
     2461 gcgatacagt aatgaccaca tcctctgcat ctttgagttt agcatacttg gttgacttgg
     2521 acttgtaagt ccgccaagga gtaaactcct tccgctccac acgcttgatt tgaccgtatt
     2581 gggtcaccgc gaaataagtt ccctcttcaa aattctccac caattcagcg aagataattt
     2641 cttcactggt atcaaagttc atgagagtct ggctcaggtg ttcaccaata tccttccaac
     2701 gaatatctgt caactcatgg acaggtcggt agatgacatt tccaagattg gtaaagagca
     2761 ataggtgctg ggttgtcttg gcattctgca agaaaatcag ctggtcgtcg tcccgcttgc
     2821 ccatttcttc caacgttgag gcattgaaag aacgcggact tgtccgtttg atataacctg
     2881 ccttggtcac gctgacaaaa gtttcttctt cgacaatcaa gctggcggtg tcaatctcaa
     2941 tcgtttctgc ctgatcttgt aactcactca aacgaggatt gccaaattgt ttcttgacct
     3001 cacgaagttc tttcttcata agattaaaca tggtccgctc atcgccgata atggctgcca
     3061 aggtctgaat ctgctcgcgt agggctgctt cttcgttttc caaggtaaca atgtcggtat
     3121 tggtcaagcg gtagagttgc aaggtcacga ttgcttctgc ctgttcctca ctaaaatcat
     3181 agctgacttt caagttttcc ttagcgtcag ccttgttttc agaagcacgg ataagagcaa
     3241 taacctcgtc caagatggaa atgacacgaa tcaaaccttc tacgatatgg aggcgttttt
     3301 ctgccttttc cttgtcaaac ttagaccgag caatgataat ctcacgacgg tgggcgatat
     3361 agctagacaa aatcttctgc aagccaacct gacgcggtgt gaaattatca atagccacca
     3421 tgttgaagtt gtaattgatt tgcaagtcgg tgtatttgta gaggtagttg agaatggtct
     3481 gctcatcgct gtcttttttc agctcaatgg caatccgaag accagtacgg tctgactcat
     3541 cacgcacctc ggcaatacca ggcaccttgt tgttgacacg gacatcatca atcttcttga
     3601 ccaaaacagc cttgttgacc tcgtaaggaa tctcagtaat gacaatctgt ttcttgccag
     3661 ccttaagttg ttcaatttca caacggctac gaacaacgac acggccctta ccagtttcat
     3721 aggctttctt gatttcgtca gccccttgga taatggcacc tgttgggaag tctggtccag
     3781 gcaagaactc catcaacttt tctaacttag ctgttggatg atcaatcata tagacaacgg
     3841 catcgatgac ctccgctaga ttatgcggcg ggatgtcagt cgcgtaccct gctgaaattc
     3901 ctgtcgctcc attaaccaag agattaggga aggcagctgg tagcacagtg ggttcttttt
     3961 ccgtatcgtc aaagttccag gcaaatggca cagtcttttt ctcgatgtcg gccagcagat
     4021 aaccagccat ttcagacagg cgagcctcgg tgtaacgcat ggctgctggc ggatcgccgt
     4081 ccatggaacc gttgtttccg tgcatctcca ccaaaatctc gcggttcttc cagtcctgac
     4141 tcatgcggac catggcatca taaatagagg aatcaccgtg cgggtggaaa ttccccatga
     4201 tatttccgac tgacttggcg gacttgcggt aacccttgtc aaaagtgttg ccgtccttat
     4261 tcatggaata aagaatccga cgttgcacgg gcttgagacc gtcacgaatg tccggcaggg
     4321 cccgctcctg aataatgtat ttggaatagc gaccaaaacg ctctcccatg atgtcctcaa
     4381 gggacatgtt ttgaatgttt gacataaatt aaaatacaaa gggacgattc agtcccgtgt
     4441 tcagttagac agactagctg aacattcctt tctttgtttc ttggtggaaa gttaaatttt
     4501 ctttcttttt tacttttttg ataatcacct taagtagtgg gaacggaaga gaccttatat
     4561 agcaatttct gttgctatat ccttaactaa acattagttc tttacctaag ttctctgata
     4621 aaagaaagca atcatatccc ctactctatc ataaagatta tcatcaacat atgctaattt
     4681 ctcaatttct aattcctcat catcaatcaa attttcttga taagcttgag catattcacc
     4741 acacgatttt agataatcca ttattaaaac taactcttgc ttctgttgag aagaaaggac
     4801 ttttccttta tagagagtct cctttatgaa tcgacagtag tttgagtgct cttcacacca
     4861 attgacattg ccatttgctt gagcttccca gcgaagtttt tctaattgtc gaagtaattc
     4921 accttgtaaa gtagaagaaa tcccgtattt aggcacatct gctcgccaaa ttgaacgtag
     4981 aacctctgta tactgagaag cataaggaac tttcttaagt tgctcgatag ttagcttttt
     5041 tcttaaaaac tttatcatag ctactcatcc actttccact ggcttgcttg ccagcttcta
     5101 aagtcatcga catctgtttt gattaatttc aaacaggcat taaggacaaa ggcatcatca
     5161 agtagacctg ctccaaggat gaaatctggt aaggcatcaa tagggctgaa aaaataaatc
     5221 aaagcaccta taattgcgag gattgtccct ttgggtaact tgctgtaatc tctacggata
     5281 tagcttcgca ccatggaaat caagcctgga atggccgcaa acttatctcc accaaaaggc
     5341 atacgagaca gtttccgttc cgtgctatgt aaaaaatgtt ctaattttcc ttgctcagat
     5401 agcaagtttt ctgcatgttg ccgagttttg gaagattgcc aaatcttcaa tgcttggtca
     5461 aatttctctt ccaactcctg tttgttttta aatggcttgc gctttcgagc ccattcagac
     5521 aaccgttttt gaaaacggtc agttaatttt ggcataaaac acctcacata gtttgcttgt
     5581 gctagagtta caaatcctct gtgcgataga gattgtatcc ttcatagtaa taagaatggt
     5641 cgatgccttt gaaataagta gcctgaccaa gatcttccgt taaggcttgc aataacagat
     5701 atttaagctc tcctgttgac acatggcttc taaccattgc attgaaatat tcatctttat
     5761 caatgctatt ccagtcaaca accttaccca aactatcccg tagcatacaa tccaaccaga
     5821 tacgcatact gcgtccattt ccttctcgga atggatgtgc aatgttcata tctgcatatt
     5881 tttcaacgat ttcgtcaaag gtttcatgag gtagcttgtc gatataggcc aaagactgct
     5941 ccagaaagat tcgtggagca aattgaaaat tatccttggc aatattaacg tcgcgaatct
     6001 tacctgaaaa gtcgtaaatc tcttcaaaca aggtctgatg aatgtgtgcc aaacctgcaa
     6061 aggttccaac ttctatctgg aataactgac cagtttcgaa cagttgagct gcttttttct
     6121 tgctgatttg ttcttcaaga cgagctaatt cagccgaatt ttcaatcccc aatttatttt
     6181 ctaaaaccat atggcacctc aacttccctg agattattta gtaaaagctg tcgcttcctc
     6241 caaggtaaac ttcacattgt cctcaatcca cttgcggcgt ggctcgacct tgtcgcccat
     6301 gaggacagat acacggcgtt cagcacgagc caggtcttca atggtgacac ggatgagggt
     6361 acgggtttca gggttcatgg ttgtttccca gagttgatcg gcattcatct caccaagacc
     6421 cttgtagcgt tggaggataa agcccttgcc aaaatccttg cggagatctt ctaattctcc
     6481 gtcagaccaa gcataagcaa tcttttctgt cttgcccttg ccttttgaca tcttataaag
     6541 tggcggaagg gcgatataaa cccgtcctgc ttctactaac ggtcgcatat agcgatagaa
     6601 gaaagtcagc aagagagtct gaatgtgggc accatccgta tccgcatcgg tcatgattat
     6661 aatcttgtca tagttgacat cttccagcgt aaagtctgaa ccaacacctg caccaatggt
     6721 gtaaatcatg gtattgattt cttcgttttt gaggatgtcc gccatttttg ccttggcggt
     6781 attgataacc ttaccacgca aaggtagaat ggcttgaaac ttgcggtcac ggccttgttt
     6841 ggcagaacct ccggccgaat ctccctcgac cagatagagt tcatttttgg ctggattttt
     6901 ggactgggct ggagtgagtt tccctgacaa aaggccctta tccttcttgt ttttcttacc
     6961 gtttcgggat tcgtcacggg ccttacgagc ggcctcgcgg gcgtcacgcg ccttaatggc
     7021 tttacgaact agatttgaag ctaattcccc attttccaaa aggaagaatg ttaacttgtc
     7081 cgatacaatg ccgtccacca ccggacgagc aagtgggctt cccaacttgt ccttggtctg
     7141 cccttcaaac tgcaaatgct cttctggcac aaggatagaa aggaccgcag acaaaccttc
     7201 acggtagtca gagccttcca agttcttgtc cttttccttg agcaagttgg tcttgcgggc
     7261 gtagtcgttc atagccttgg taatggctag tttgagacct gtttcgtggg taccgccgtc
     7321 cttggtccga acgttgttaa caaaggacag gatgttgtct gaataaccgt cgttgtactg
     7381 catggccact tgaacttgga atcctgcatc ttctccttca aaatagagga ctggtgtcag
     7441 agtttccttg tcttcgttga ggtaggaaac aaagtcctga acgccatttt cgtagtggta
     7501 ctcctcctgc tcacctgttc gctcatctgt cagagtcatg gtgacttgtt tgagcaagaa
     7561 ggctgattct ttcaggcgtt cggcaatggt attgaacttg aagtccgtcg ttgaaaagat
     7621 ggtatcgtca ggcataaagg taacttttgt acctgttttt gattttggag cggtgccaat
     7681 cttctctagg gtcgtgactg gctttccgcc ctgctcaaac cgttgcttgt aaacagctcc
     7741 gtcacgggta atctcaactt ccaaccagct agacagggca ttgaccaccg aagaaccaac
     7801 cccgtggaga ccacctgaag tcttgtagcc accttgaccg aacttacctc ctgcgtggag
     7861 aacggtgaag ataacttcga ctgttggttt gcctgttgcg tgcataccaa caggcatccc
     7921 acgtccacgg tcggaaacag acaggctacc atccttgtta atagtcacgt caatccgatc
     7981 accgaaacca gacaaggctt catcgacagc gttatcgaca atctcccaga ccatgtggtg
     8041 aagaccattc ccgtctgttg agccaatgta cattccaggg cgtttgcgaa cggcatctag
     8101 gccttccaat acctgaatgg catcatcatt gtaattattt atatcaatct ctttcttagc
     8161 caaaattgac ctccatactt atcatccttt ctatattaca agttttttag catttttgca
     8221 aaatatttct gttacctatc ggctgagaca actcacaaaa taagattttg gactaaatta
     8281 gatgaaaata tgctataatg aaaacatgtt agtcaatctt attttactat ttattgcata
     8341 tttgttggga tctatcccgt caggactttg gataggtcaa acttttttca atatcaacat
     8401 tcgtgaacat ggttcgggaa atactggaac aaccaataca ttccgtattt taggtccaaa
     8461 agctggtacg attgtatttg taattgattt tctcaaggga acgttggcag ccttgcttcc
     8521 gctgatcttt catgcccaag gaatttctcc gattgtattt gggttaatgg ctgtactggg
     8581 gcacactttc cctatctttg cccaattcaa aggtggaaag gcggttgcaa catctgctgg
     8641 tatgcttccg ggcgtcgcac ctgctttctg tctctatcta gtcatcattt ttgtgacttc
     8701 tctttacctc acttcgatgg tttccttctc tagcgtctta gcagcagctt tagcaatctt
     8761 aggagctctg ctatttccag caataggaat catcctgcca agctacgact ggctctttac
     8821 agtgattatc gtctttcttg gcagttttgt catcatccgt cacaaggaaa atatccagcg
     8881 gattttgaaa aaagaagaga accttgtccc attcggactc aatctaacca aacagaaaaa
     8941 gcgagcttag tactcgcttt tagtttacct tgacttcgac cacttctatt ctctccccac
     9001 caagcaaaat caaatggcag tctacacgat ctatcttgta ggcttttttc aatgcctcgg
     9061 catagagttg catttgagct tggtaacgtt ggctgagttg gcttggttgg tcatatttgt
     9121 ctgtcttgta atcaaagagg acaatatgat catcatatag taggtagcca tctataatcc
     9181 cacgaaggac aaagttttcc tgagatacag aatctgtctg caaacttgca aatggtgctt
     9241 ctcggtggag tttgtccgta ttggctagaa tttctcttcc taagtctgta tcaaagaaat
     9301 ccaaaatcat ctgaacattg attttatctt taatcgcttg ctcagcatga acagcttcta
     9361 gggcttctct taccgtatct tctgtcacca accaagacag atccaagcgt tgcatgagtt
     9421 catgcactgc tgaaccgact tgggctccag tgattttagg tttcttggag aaatctggca
     9481 agttgaaggt ccgttttggc tggtattttt ccataacttc catgccttct tgttccaaaa
     9541 taggctcata gagtttcttg atttgacttg gagtccgtaa acttggcaat tcaatggcaa
     9601 ccttatagcg ttcgttcaac tcttgaacag aagacaactg gtccaaggcc tgagcaatgt
     9661 cttctgattg acgaatatct ttaaggctgg catcttctag cttactcttc aatgtaagtt
     9721 ttccaatctt ctcctctgtc aaatcctcat ccgtcacaaa gactttcttg aagtgcaagt
     9781 cttctcctga gaaggcctca tcaatggcta gaatccagtc ttggaaggtc gccacgcttt
     9841 cacgagttga ttgggctagt acgccatctg cctttttgcc atcgtatttt ttagctaact
     9901 tatcagcatt gccctttcca accagataca gcttcttctc ggcacgcgtt agtgcaacat
     9961 aaagcaaccg catttgttct gaaagattgg caatttttag ctcttggaga ttatcttgat
    10021 aaggaagggt gttcattaag actcgcacct gaggtaatgg gctattcacc ttatctttca
    10081 tatccgctaa atattggata cctagaccat ttttccggct gatgatgact gactggtaat
    10141 ggtcttccaa gttgaatcgt ttatcgatgt tcatgaggaa gacatatttg aactctagac
    10201 cttttgactt atgaatggtc atcagctgaa cagcattttg cggtagaaac tcttgtacat
    10261 ctgccaagtc cttgtcattt gctaaggcac ggtcaatcat gacgatgaaa cgagacaagc
    10321 ccttgtagcc tgttttttca aactggttgg cacgcaaacc tagggcatat agatttgcct
    10381 gacgcttgct gccatttgga agagcaccga catagtcata gtagaatttt tcattgaaaa
    10441 tcttccaaat caggtcataa atagaatgca ttttggcaaa agcacgccag ttttccaaaa
    10501 tagataagaa atccttgatt ttcttcttca actctcctga cataagttca ggatgttgcc
    10561 catttgtttg ctgggctatt tccattttct ggtaaaagaa acctgtccca gcctgcaagg
    10621 aaatccgtgt cagctcatcc tcatcgaatc ggaacatagg cgatttgaga agagcaacca
    10681 aggcatagtc attgagtgga ttattgataa cccgcaaggt atccagcata atcatgactt
    10741 ctaaagattg cagataggag gctgctccgc catctgccac aattggaatc ccatgttttt
    10801 caaaaatgga tataatgagg tcattgtgcg tccgtttttg caccaagaga gtgatgtcct
    10861 tgaagtctgc tccttcttca ttgtgaagac gaataatctc ttttgcaaca acctcgattt
    10921 caccagcagt cagaggtgtt tcttcctcag catccgttga agagtttgcg ctatcctgct
    10981 ggtcgtaaat caagtattcc atctcatgct tgggctgggc aattttttga ccaggactac
    11041 cagccactag actgtgggtt tcatcatact tgatttcccc tacttgacgg tccatcaggc
    11101 gcgtgaaaat agcatttgtc gcctccaaga cctcaatctg actacggaaa ttttccttaa
    11161 gcaaaatcaa ttttccagcc tgagaatctg cctggtaaag ttcaaatttc tcttggaaaa
    11221 tcatcggatc cgcctgacgg aatcgataga tggactgctt aatatcgccc accataaagc
    11281 ggttgtgacc gttagacagc aggtccagca ttcgctcttg gctatggttg ttatcctggt
    11341 actcgtccac catgacttcg tggtattttt cttggaaaaa ctggcgaatg tccacatttt
    11401 cttctaaaat acgaatagcg aaatgcccaa tatcgccaaa ttcaaaagct gcttccttga
    11461 ttttgacatc cagataagcc tgcgaaaaat ccaagacaaa gtcacgtaat aatatcagca
    11521 ttggcaaagc ttgaatctga tagtctttca gcagggttaa ttcataaact tcctgcccca
    11581 aatctcgtag tccagagata atctggctct tgccagcatt gtattcttct ttaaaggcta
    11641 tcaaatgctc atcttttggt cgattggcat tggtcagacc cttgcctctg gactggtcgt
    11701 tgataagaag gactttttca acccgctcca tcaagtcctt ctggtccaga ctatctaaac
    11761 ctgtcagcaa atctaaaact tcttccacat ttgcaaaata cttggcggaa ccaaagtcat
    11821 ttctgcccca ttccacatgg tagcggaaga aatctgcagc ttgatagagc ttgtctaaca
    11881 ctttttcctt aaagccttgc tctaagatca gctctatacg ctcttgactg tacaaatcag
    11941 cttgaacagc ctgctctttc agccatttag ttggactgct tgtcgattgg ctaaaatcat
    12001 gcacctgata aacaacctga cggaaaccac cattatcctt ccggttgcct gaaaaattgc
    12061 gtaccaattt tcggaatgca ccattttcat cctgttcaag ataatctgca aagagctggt
    12121 caaaaacctc ttttttcaga ctcgcttttt ctgtctcatc ctgtaaaatc cgaaactgtg
    12181 gagaaatgcc aaggctataa ccgtaagtcg ttaccaattt ctgcgtaaag gagtccattg
    12241 tcccaatatc cgccttggtc aaatccgcca actgggctga caaatgccga cgcaagtcca
    12301 tatccatcgt ctccgcaatg gtttcattga gatttttctc gatccgctcc ttcaactcac
    12361 cagccgcttt gactgtaaag gtcgagataa agagctgatc aatccctacg ccacgcttca
    12421 acttatccaa aatccgttga accatgacga aggtctttcc tgacccagcc gatgcagaga
    12481 ccagaacatt ctgaccgtgc gtgtagatgg cttcaatctg ctcaggtgtg cgtttttgtt
    12541 gcttatcaga attggcttcc gccagctgca cagccttgat ttcttctgcg cttaaaaatt
    12601 gttcaaaagc cattagtcct gacctccttt cattcgttcc atccaatctt gacgcttggc
    12661 ctccttgacc aaccgccgtg ccatgcccat gtgccgatcc gcctcaaaac cagtgattgc
    12721 cttcagctgc tcacctgcca ccgaacgccc atctttggta taagggttaa tagcaaagcg
    12781 accagccaga atgtccatgg cagcttgttt gtagagttct tggttgtggt tcaataaaac
    12841 cgcaaactca tcttccgtgt agagttggtt acgagtctga taaaaatgat tgagccccag
    12901 actttcctcc ttcaagaaaa gccccttata aaccaagcta gtattggcag ctccttctag
    12961 ctgttctaaa ttcttcgtgt cttttaattt gataatcgga tcctgcaaat gcaaatacat
    13021 agctccaaaa acaggtttat ccttggtttc atccagctct tgtaaggctg ccaagtaggt
    13081 taccaactga ggttttagac cattgtaaaa atccccaact gaaaaagatt ggtcgctgga
    13141 cttgtaatcc acaacaccaa cagcctgatt gatttgtaag gtatccaagc ggtcaatggt
    13201 accatttaca tgcacactct tgccattttg aaggtcaaaa actagcgcct tatcctgacg
    13261 gaaactcttt tcctgaccat caatttcaac caaatcatta tcccgcaaaa tagtcgcact
    13321 ggaacgagca atcttatcca gcacctcctc cgtataacga gcatccgcat cttggttata
    13381 aaacatagca aattctgcct cgtcacgcgt acgcaggatg gccttatcca ctttttgatc
    13441 aaaatctagc tcagattggt ccatgaccac tcgttcaaaa atccgatgca agaagagccc
    13501 gtgttgaaag gccgtcggat ggatggattc ctgttcccgc aagcgaagaa cattacggac
    13561 aaagtaaaga tattggttat tgtagaaatt ggtcaagctg gatgcagaaa gattgagcgg
    13621 cttatcctct ggatataaag cagccaaggt atcagctgca agctgtttag aagtaatatc
    13681 accagtaatg gtcggaattt caatgccttg cgactccaat ttcttcttca aatggcgaac
    13741 caagactgtc cagaaggccc tctgcccttc ccattcttca ttatcaagag atggtctttc
    13801 tgactcaatc aaacgggata agagactctt gtagtgaccg atgtcttgcg gactaagact
    13861 tttaatccga ccacgttctt ctgacgaaag ccccatttct tttagaattt ttacataagg
    13921 agaaagacta tcttcgcctt cattataaat ctgaggagtg gaaataacta actgttccgt
    13981 cgcagcattt agcagagaca tcatggcagc atggttcttc ttaatattct ctcggctgac
    14041 caaatcgaaa cgtgaagaac ttgccgaaac aagattgact ttttccatct cttcttccgt
    14101 caacagactg gtattcttgg caacttttgg aaaattggac tggcccattc cgatagcata
    14161 aacatacttt gcagtgtgtg gctcaatcaa atcataggat ttgacattga ccacatcaac
    14221 cgttgcagga acggtgcgat agtgactggc ctgcatgcca gcctgtaaaa tagccagaaa
    14281 atcatccatt ttcaattttt cttttccgaa aatctgatga aaattctcaa gaagatgggt
    14341 aaaacttttc cagacctgct cttctttttc ttgctctact tcactcaaat ttcgagacat
    14401 tttttccata tttttaggaa actcaattgc ctccaaaaat gccataaatt tctcaagcaa
    14461 ggtagctcca gattgtggtc cagccttaaa cagctgattc aaaggctcca taatcttagc
    14521 acgtagaggt tctaagacag ccaaatcata gacaagcctt ttctccttaa cgacttcagc
    14581 gtcatagtcc gctctgccat tgaccgaaaa agccctcgaa aaagcagcct gccccttcat
    14641 atccgcaaag aggatgtagg actcaaacaa gtccagctcc ttttgggaaa tgctggcgta
    14701 caaaccagat ttgaggagat tgagcaggtc ttccgcacgg aaacgatagc gacgcaagcg
    14761 ctccaaggac tccacaaagt ggactagggg atgatggctc atctcctctg ccttaccaaa
    14821 atagtaggga atatcatatt tgtcaaaaat tttgccaatc tgcaacttgt agctatccac
    14881 atcacccaag agcaacaaga tgtctttata gcgagctcct tggtgtatgt gttggcgaat
    14941 tgccgttgct acctgctcca cctcttcttt ttggttaacg acttcccata gctggatttt
    15001 ttcttgatct gctggagtca agtctatcat ggtcccgctg tagtcataat aggcttccat
    15061 attctttgaa aatttaccaa tactgtccaa gactggctct tgaccaatat aagtagcctt
    15121 agtctggaac tgcgctgata gctgacgtaa aaagtcaacg ttggcttgat agacattgcc
    15181 ttccgcatag atgacttgca cagctttctt gctggcatat acaccaatta ggatttccga
    15241 cacccgttca tttaaagcac ttactaaagc ttcttcttct gccgaaaaac gagaaaatcc
    15301 gtctaccacc agcacaatgt tcttcaactg ctcatccaac tgaccagttt ctaccaagcc
    15361 agtcaactgt gctaacttag actgatgctc aaagctttct ttggacaaaa tgtctgttac
    15421 agccagaaaa atctttacca aatcagcctg cttatctggt gagtccatgg cttccaagtc
    15481 caaaatagac atatttgccc gttgcaattc tttgtataaa gcaactagct ggttaatgaa
    15541 accaaaatct ctctgcaaac gaccatagac tttcaagtcg ccatcttcaa attgcgatag
    15601 aacacgaaag aaaatcattg ccagaccaac atcatccagc ggttgacctg cttctttcct
    15661 atcaatcatc aaatagcggg ccaattgccc aaaacgggtc acgataatat caaaagttgc
    15721 ctgttctggc aggtattcca agaccttgcg ttccatctca aaggatagag agtttggagc
    15781 aatataaaaa actcgcttag cttccttagc aaattctgcc gaggtatccg ttaaatactg
    15841 cgtcagagga ttgcgaatat ctgtatagac taatttcata tcaaattctc atgctttcgt
    15901 tgaatatact atatctagta tatcaaaata agacaaaaaa atgggagcga gaaagaactc
    15961 gaccgaataa aaaaagagct cgtctcccac cccc
//
LOCUS       DASFSW010000033        15992 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.033, whole genome shotgun sequence.
ACCESSION   DASFSW010000033 DASFSW010000000
VERSION     DASFSW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.033"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(6..866)
                     /locus_tag="U1099_000813"
     CDS             complement(6..866)
                     /locus_tag="U1099_000813"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEM4867147.1"
                     /translation="MKIIIPATSANIGPGFDSVGVALSKYLTIEVFEETDEWVIEHNL
                     EHVPSDKNNLLIKTALKIEKGLQPHRIRMISDIPLARGLGSSSSVIVAGIELANQLAG
                     LNMTADEKLLKATEIEGHPDNVAPAIFGNLVISSYVNKKVQAVVTEFPEASFVAFIPN
                     YPLRTVESRGVLPSQMGYKKAVAASAIANVAVASLMAGDLEKAGKAIQSDMFHEPFRQ
                     LLVKEFCPIKQTAQELGAYATYLSGAGPTVMVLAPKDREDAIVLALEELNLDGTVHRL
                     QVDTKGIAIV"
     gene            complement(870..2156)
                     /locus_tag="U1099_000814"
     CDS             complement(870..2156)
                     /locus_tag="U1099_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEM4867148.1"
                     /translation="MVVKIGLLGFGTVASGVPFLLEENQEKIEKAAHDSIEVAKVLVK
                     DEAEKDRLLAAGHDYHFVTNIDDILADEDIAIVVELMGRIEPAKTFITHALEAGKHIV
                     TANKDLLAVHGSELRALAEEKGLALYYEAAVAGGIPILRTLVNSLAADKVTRILGVLN
                     GTSNFMLTKMVDEGWTYEKALQTAQELGYAESDPTNDVEGIDAAYKAVILSQFAFGMT
                     VDFEHVAHKGISNITPEDVAVAQELGYVIKLVGDIQETTSGIAAEVSPTFLPKNHPLA
                     SVNGVMNAVFVESIGIGQSMYYGPGAGQKPTATSVMADIIRIVRRLKDNTIGKAFNEY
                     SRSLQLAAPSDVKSEYYFSIDAPDKNGKMLRLAEIFNSEEISFKQILQQAADGETARL
                     VIVTHEMSKTQLENVTDKLSKETDFTVLNTFKVLGE"
     gene            2261..3184
                     /locus_tag="U1099_000815"
     CDS             2261..3184
                     /locus_tag="U1099_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM4867149.1"
                     /translation="MKHSRRARRSRTQKKQLVPMLLGIALLVVTLLSIFLIFLKQPSK
                     QQSDSKASTVETTTTSSVMTSESSEVQTEIIWEQQTEPIKLPILMYHAIHVMAPEEEA
                     NANLIVDPTTFESHLKALQDAGYYTLSPEEAYKVLTENVLPAGKKVVWLTFDDSLWDF
                     YSYAYPLLKQYQMKATNNVITGFTEYGQAGYLTLDQIKEMQTAGLSFQGHTVNHPDLE
                     YSSIEDQTNELTSSKAYLDSQLNQETIAIAYPGGRYSADTVALTEQAGYKLGVTTNNG
                     LASASDGLLTLNRVRILPTTTAENLLAEIAY"
     gene            complement(3228..4997)
                     /locus_tag="U1099_000816"
     CDS             complement(3228..4997)
                     /locus_tag="U1099_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867150.1"
                     /translation="MKNRSVFTRVWDYLRRYQSSVFLAIFLKTISAVMNALEPFVLGL
                     IITELTKNLLDIANGVEGAQINYSYIAIMLAIYALRALLYEIGAYGSNYFMTKAVQGA
                     TKELRQDLSHKINKIPVSYFDKHQYGDLLGRFTSDVETVSNALQQSFLQLVNAVLTLS
                     LAIFMCFWLDVSLALVVITLVPVTYFGSKFVMGKSQPYFKQQADALGRLNGFVQENLT
                     GFNVLKLYGREEISTEEFRQITADLQEVGFKASFMSGLLMPLVHGFSNIAYVVVALLS
                     GLKVLAGTLTVGNMQAFVQYVWQISQPVQTLTQLAPQLQSAKSSLERIFSVLDELDEE
                     DANALELTESLTGQVSFEQVEFGYSEDKPLIRNFNLDVKPGEMVAIVGPTGAGKTTLI
                     NLLMRFYDVTSGAIKVDGQDIRNLSRQSYRSQFGMVLQDAWLYEGTIKENLRFGRLDA
                     TDEEIVEAAKAANVDHFIRTLPGGYNMEMNQESSNISQGQKQLLTIARALLADPAILI
                     LDEATSSVDTRLELLIQKAMKRLMKGRTSFVIAHRLSTIQEADKILVLKDGQIIEQGN
                     HESLLADKGFYYNLYQSQFSESK"
     gene            complement(5006..6742)
                     /locus_tag="U1099_000817"
     CDS             complement(5006..6742)
                     /locus_tag="U1099_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867151.1"
                     /translation="MNLIWTYLKKYPKWIALDLLGAFLFVVVNLGLPTFLARMIDQGI
                     TKNDVSQLYFWAGMMGLIVLLGIVGRVTLAYAAGKLTTNIVKDIRNDLYEKIQDYSHH
                     EYEQIGVSSLVARMTNDAFVLMQFSEMVLKLGIITPLMMIASVIMTLVTSPSLAWTVA
                     VAMPFLVFVIFYVATKTRPLSEKQQKRLDTINQYVRENLMGLRVIRAFTREEFQENRF
                     AEVNEEYTDISKKLFNLTGLTEPLFVQIIIAMIVAIVWFALDPLKTGSLQIGDLVAFI
                     EYSFHALFSFLLFANLFNMYPRMSVSSQRIQKVLDMPISISKNEEGITKSDTRGYLEF
                     ENVTFAYPGETESPVLHNISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGRIL
                     VDGVDVRRYNLKALRSKIGFIPQKALLFTGTIAENLKYGKMDASLAELHDATDVAQAK
                     EFIESKEEKFDTHLAEGGSNLSGGQKQRLSIARAIVKQPDIYIFDDSFSALDYKTDAI
                     LRSRLKEVTENATVLIVAQRVGTIMDADQIIVLNEGEIVGRGTHNELMESNEIYREIA
                     NSQLNRQSLTEE"
     gene            complement(6818..7060)
                     /locus_tag="U1099_000818"
     CDS             complement(6818..7060)
                     /locus_tag="U1099_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEM4867152.1"
                     /translation="MDMIENLIIAIVKPLISQPDSLTIKIVDTPEFLEYHLDLDQSDI
                     GRIIGRKGRTISAIRTIVYSVPTSDKKVRLVIDEKE"
     gene            complement(7082..7354)
                     /gene="rpsP"
                     /locus_tag="U1099_000819"
     CDS             complement(7082..7354)
                     /gene="rpsP"
                     /locus_tag="U1099_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEM4867153.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLL
                     AENSVTLKEERVLEWLAKGAQPSDTVRSLLSNAGVLKKFHEQKFSK"
     gene            complement(7479..7787)
                     /locus_tag="U1099_000820"
     CDS             complement(7479..7787)
                     /locus_tag="U1099_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEM4867154.1"
                     /translation="MKNIMEARKRVEEMEKIFNRQLELNQSLSDNMAQLNQEQSTYLQ
                     LLDYYQSQTYMEDLDLSNKGYFNGIPCGVLSEDGVYNLLFDRTNLASQLRELADMLEQ
                     "
     gene            complement(7787..8434)
                     /gene="pcp"
                     /locus_tag="U1099_000821"
     CDS             complement(7787..8434)
                     /gene="pcp"
                     /locus_tag="U1099_000821"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEM4867155.1"
                     /translation="MKIIVTGFDPFGGESINPALETIKSLPKTIAGAEIILVEIPTVF
                     DKAADVLEEKMAEHLPDAVLCIGQAGGRVDLTPERIAINQDDARIPDNEGQRPIDRTI
                     REDGQPAYFSTLPIKAMVEAIHRIGLPASVSNTAGTFVCNHLMYQALYLAEKQFPKTK
                     AGFLHIPFLPEQVVDKPGLASMSLNDIVRGVEVAIGAIVEYRDKEDIKKGGGSTH"
     gene            complement(8431..9186)
                     /locus_tag="U1099_000822"
     CDS             complement(8431..9186)
                     /locus_tag="U1099_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM4867156.1"
                     /translation="MIVTTSLGMDEGLVYRARRIASELGIEYKERKKQSVGKMLGTYE
                     AVLVLYKDKLILEQRGGQVLFFHPDTAMLRIKSGRDPLLELLGKEKQSIIDCTMSLGS
                     DSIVLASAGHRVTALESSKLVHFIVSRGLQDFDSGLQEVNRAMKSIQTIWTDSLTYLK
                     EQKDKSVDVIYFDPMFSEEIKESQNLSGLSTLADRSRLTEEIVSEAKRVARKKLIIKA
                     HFRDQVFEEFGFKRHVRPNQKFHYGEIILEEEV"
     gene            complement(9250..12429)
                     /gene="carB"
                     /locus_tag="U1099_000823"
     CDS             complement(9250..12429)
                     /gene="carB"
                     /locus_tag="U1099_000823"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEM4867157.1"
                     /translation="MPKRTDIKKIMVIGSGPIVIGQAAEFDYAGTQACLALKEEGYSV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKAGILDELGVELLGTKLSAIDQAEDRDLFKQLMEDLNQPIPESTIVTTVDEALEFAA
                     EIGYPVIVRPAFTLGGTGGGMCANEEELREIAENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLSIIRALK
                     IEGGCNVQLALDSHSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     VNPVTGTTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVYHNEMPELSQVTDDQLVEKIVKAQDDRLFYLSEALRRGYTVEELAQ
                     LTKIDLFFLDKLLHILEIEQELATNFDNIDLLKKAKKYGFADRKIAELWGRTESYIRQ
                     LRTEHKIVPVYKMVDTCAAEFESATPYFYSTYEWENESIRSEKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTLEDVLNVIDL
                     EKPKGVIVQFGGQTAINLAEPLAKAGIPILGTQVADLDRAEDRDLFEKALKDLGIPQP
                     PGQTATNEEEALEAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPEH
                     PVLVDSYIVGRECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSKEVQA
                     TIADYTKRLAIGLDCIGMMNIQFVIKDETVYVIEVNPRASRTVPFLSKVTDIPMAQVA
                     TKLILGQSLAELGYQDGLYPESNQVHVKAPVFSFTKLAKVDSLLGPEMKSTGEIMGSD
                     LTLEKALYKAFEASYQHLAEFGNVVFTIADEDKEEVLGLAQRFYELGYGLLATAGTAA
                     FLTENGLAVTTVGKLGDDSRPDIPSLVRAGKVQAIINTVGKKRVADGDGQVIRSSAIE
                     GGIPLFTALDTAEAMVKVLESRSFMTQAI"
     gene            complement(12939..14018)
                     /gene="carA"
                     /locus_tag="U1099_000824"
     CDS             complement(12939..14018)
                     /gene="carA"
                     /locus_tag="U1099_000824"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEM4867158.1"
                     /translation="MSKRRLILENGTIFEGEAFGADIDVTGELVFSTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDFESIKPTCKGVVVSEWARRASNWRNQMTLDEFLK
                     AKKIPAISGIDTRALTKIIRQHGTMKATLANVGDSVEHLTDQLRATVLPTNNIQQVST
                     KTAYPAPGVGRSVVLVDFGLKHSILRELAKRDCNVTVVPYDTTAEEILALNPDGVMLS
                     NGPGNPDDVPEALDMIRGILGKIPIFGICMGHQLFAKANGATTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVAREDLPECLMITHEEINDKSVEGVRHKYYPGFSVQFHPDA
                     APGPHDASYLFDEFMELMDSFKKGQ"
     gene            complement(14266..15189)
                     /locus_tag="U1099_000825"
     CDS             complement(14266..15189)
                     /locus_tag="U1099_000825"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEM4867159.1"
                     /translation="MTITNGKVSLKHLVTMETLSNEEVLGLIQRGIAFKRGEKVELDR
                     KYYASNLFFEDSTRTHKSFEMAELRLDMGMIDFDARTSSVNKGETLYDTILTMSALGV
                     DICVIRHSEVDYYKQLIDSPTIQTSIVNGGDGSGQHPSQSLLDLMTIYEEFGTFEGLK
                     IAIAGDITHSRVAKSNMQILKRLGAEIFFAGPEEWYAEEFDVYGQHLNIDDIVETVDV
                     LMLLRVQHERHDGDGGFSKETYNRLHGLTEERYKRLKDTAIVMHPAPVNRDVEIDDSL
                     VEAPKSRIVRQMQNGVFVRMAILEAIVNGKA"
     gene            complement(15245..15766)
                     /gene="pyrR"
                     /locus_tag="U1099_000826"
     CDS             complement(15245..15766)
                     /gene="pyrR"
                     /locus_tag="U1099_000826"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEM4867160.1"
                     /translation="MKTKEIVDDMTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     IAKRIQERLKQLEGIDVPLGELDTKPFRDDMKVEDDTTNMTANVNDRDVILVDDVLYT
                     GRTIRAAIDNIVSLGRPARVSLAALVDRGHRELPIRADYVGKNIPTSRSEEIIVHMAE
                     IDGQDAVLLVEGA"
BASE COUNT         4689 a         3761 c         3008 g         4534 t
ORIGIN      
        1 actatctata caatagcaat ccctttggta tcaacttgaa ggcggtggac agttccgtct
       61 agatttagtt cctctaatgc tagaacaata gcatcctccc tatctttggg cgctaatacc
      121 atcactgttg gaccggcacc tgataagtag gttgcatagg ctcccaactc ctgtgcagtt
      181 tgcttgattg gacaaaactc cttaacaagt agctgacgaa acggttcatg gaacatgtct
      241 gactgaattg ctttcccagc tttttccaga tcacctgcca tcaaactggc aacagcaacg
      301 tttgcaatgg cactagcagc cactgctttc ttgtacccca tctgagaagg cagtacacct
      361 cggctttcaa ccgtacgcag tgggtagttt ggaataaagg caacaaaact tgcttctggg
      421 aattctgtta caactgcctg tactttctta ttaacatagc tagagatgac taaattacca
      481 aaaatcgctg gggctacatt atcaggatgt ccttcaattt ctgtagcttt gagtaatttt
      541 tcatccgctg tcatattcaa acctgctaat tgattagcca attcgatacc agctacgata
      601 acggaactgg atgaacccaa accacgtgcc aagggaatat cactaatcat tctgatacga
      661 tgtggctgca atcctttttc aatctttaaa gctgtcttga tcaaaagatt gttcttgtct
      721 gatggaacat gttcaagatt gtgctcaatc acccattcat cagtctcttc aaatacttca
      781 atcgttagat atttcgaaag ggcaacacct actgaatcaa atcctggacc gatattagct
      841 gaagttgctg gaataataat tttcattgct tattctccca gtactttaaa ggtatttaag
      901 acagtaaagt ctgtttcttt actcaatttg tctgttacat tttccaactg agtcttactc
      961 atttcatggg tcacaatcac taggcgggct gtttctccat ctgcagcttg ttgcagaatt
     1021 tgcttgaagg aaatttcttc agaattgaag atttctgcta aacgaagcat ttttccattt
     1081 ttatcagggg catcaattga gaaataatat tcgcttttca catcgcttgg ggctgccaat
     1141 tgaagtgagc ggctatattc attaaaagct ttgccgatag tattgtcttt caagcgacga
     1201 acgatacgaa taatgtcagc cataacactt gttgcagtcg gtttttgccc agcacccggt
     1261 ccatagtaca tagactggcc gatgccgata gattcgacaa atacagcatt catcacacca
     1321 ttcacgcttg caagtggatg atttttcggt aggaaggttg gagatacttc ggcagcaata
     1381 ccagaggtag tttcttgaat atcacccaca agtttgatga catagcccaa ctcttgtgcc
     1441 actgctacat cttctggcgt gatatttgaa atacccttgt gtgctacgtg ttcaaaatca
     1501 actgtcatcc cgaaagcaaa ttgactgagg ataacagcct tgtaagccgc atcaattccc
     1561 tcaacgtcat ttgttgggtc agattctgca tagccaagtt cctgtgctgt ttgaagagct
     1621 ttttcgtaag tccagccttc atcaaccatc ttagtcaaca tgaagtttga agtaccattc
     1681 aatacaccga ggatacgagt caccttgtca gcagccaatg agttaaccaa ggtacgcaag
     1741 attggaatac cacctgcaac tgcagcttca taataaagcg caagaccttt ctcttcagcc
     1801 aaggcacgca attcgctacc atgaacagca agtaaatcct tattggctgt tacaatatgc
     1861 ttgcctgctt ccaaggcatg tgtaataaat gtttttgctg gctcaatacg tcccatcagc
     1921 tcaacgacga tggcaatgtc ctcatcagct aaaatatcat caatatttgt tacaaaatga
     1981 tagtcatgac ctgcagccaa gagacggtct ttttctgctt catccttgac caaaacctta
     2041 gcaacttcaa tactatcatg cgcagctttc tctatttttt cctgattttc ttctaataaa
     2101 aatggcacac cactcgcaac ggttccaaat cctaataatc ctatcttaac taccatgtgc
     2161 tctcctttaa aatgtatttc tcccattata acaaatattc tgactatttg gcaaaaaatt
     2221 tgatataatt gttttaatat atgattatgg agataagata atgaaacatt caagaagagc
     2281 ccgtcgaagc cgcacacaaa agaaacaact tgtgccaatg ctacttggca tcgcattatt
     2341 agtagtgact ctactatcta tttttctaat ctttctaaaa cagccatcta agcaacaaag
     2401 tgattccaaa gcttcaacgg tagagacaac aaccacttca agtgttatga cttcagaaag
     2461 tagtgaagta caaacagaaa tcatttggga acaacaaact gaacctatca aacttccaat
     2521 cctcatgtat catgccattc acgtaatggc tcctgaggaa gaagctaatg cgaatcttat
     2581 cgtagatcca acgactttcg aaagtcatct aaaagcattg caagatgcag gttactatac
     2641 tttatcacct gaagaggctt ataaggtatt gacagaaaat gtccttcctg ctggcaaaaa
     2701 ggttgtctgg ctaacctttg acgatagcct ctgggatttt tatagttatg cctacccatt
     2761 gcttaagcaa tatcaaatga aagcaaccaa caatgtcatt acaggattta ctgaatatgg
     2821 acaagcaggg taccttactc ttgatcaaat caaggaaatg cagaccgcag ggctctcctt
     2881 tcaagggcat accgtcaacc accctgattt agagtatagt tctatcgagg atcaaacaaa
     2941 tgaacttact tcctctaaag cctatcttga cagccaatta aatcaggaaa ccatcgctat
     3001 cgcctaccct ggtggacgat actcagcaga tacagtagcc ttaactgaac aagctggcta
     3061 caaacttggt gtgactacta acaacggctt ggcttctgct agtgacggat tactgactct
     3121 caatcgtgtt cgaattctac cgaccaccac cgcagaaaat ctactagctg aaatcgccta
     3181 ttaaacacta aactcccctg tcttcactga caagggagtt cgttttttta tttggattca
     3241 ctaaactggc tttgataaag attataatag aaacctttgt ctgccaaaag actttcatgg
     3301 ttaccttgtt cgataatctg tccatctttc aagaccaaaa tcttatcagc ttcctgaatg
     3361 gtagataagc gatgagcgat gacgaaactt gtccgccctt tcatcaaccg tttcatagcc
     3421 ttttgaatca agagttccaa acgggtatca actgaagaag tcgcttcgtc aagaatcaga
     3481 atagcaggat ccgccaacaa ggcacgcgca atggtcaaaa gctgtttctg accttgggaa
     3541 atattgctgg attcctggtt catttccata ttataaccac caggcagggt acggataaaa
     3601 tggtcaacgt tggcagcttt agcagcctcc acaatttcct catccgtcgc atccagacgt
     3661 ccaaaacgca ggttttcctt gatggtccct tcatagagcc aagcatcctg caagaccatg
     3721 ccaaattgac tacggtagga ttgacgtgaa agatttcgaa tatcctgccc atctaccttg
     3781 atagcacctg atgtgacatc atagaaacgc atgagcaagt tgataagggt tgtcttacct
     3841 gcccccgttg gtccaacgat agcaaccatt tcacctggct taacatcaag attaaagtta
     3901 cggataagcg gcttgtcttc tgaatagcca aattcgacct gttcaaagct gacttgtcct
     3961 gtcagacttt ctgtcaactc cagcgcattg gcgtcttctt catcaagctc atctaggact
     4021 gaaaagatgc gttcaaggga agatttggca gactgcaact gtggtgccaa ttgggtcaag
     4081 gtttggactg gctgagaaat ctgccagacg tattggacga aggcctgcat attacccacc
     4141 gtcaatgtac ctgcaagaac tttcaatcca gatagcaggg cgacgacaac gtaggcgatg
     4201 tttgagaagc cgtgaactag aggcatgagc aaaccagaca tgaaactagc cttgaaacca
     4261 acttcctgca aatctgcggt aatctggcga aactcttctg tagaaatttc ttcacggccg
     4321 tagagtttga gaacgttaaa accagtcaga ttttcttgga caaagccgtt caaacgacct
     4381 agggcatcag cctgctgctt gaaatatggc tgggatttgc ccatgacaaa cttagaacca
     4441 aaataggtca ctggaaccaa ggttatgact acaagtgcta gggatacatc caaccagaaa
     4501 cacatgaaaa tggctagaga gagggtcaga acggcattga ctagctggag aaaactttgt
     4561 tgcagggcgt ttgaaacagt ctccacatcg cttgtaaaac gtccgagtaa atcaccatac
     4621 tggtgcttgt caaaataaga tactggaatc ttgttaattt tgtggctcaa atcctgacgt
     4681 agttccttag ttgctccctg cactgccttg gtcataaaat agttagaacc gtaggcaccg
     4741 atttcatata gaagggcgcg aagggcatag atggcaagca tgatggcaat gtaggaatag
     4801 ttaatctgcg ctccttctac tccattagca atatccaaca aattcttggt caattctgta
     4861 atgataagcc ccagcacaaa gggttctaag gcattcataa ctgcactgat ggtctttaga
     4921 aaaatggcta aaaatacaga agattggtaa cgacgcaaat agtcccaaac acgggtaaat
     4981 actgatctat tcttcataat tcctcctatt cttctgttaa cgattgacgg ttaagttgag
     5041 agttggcaat ttctcggtaa atctcattgc tttccatcaa ttcattgtgg gtgccgcggc
     5101 caacgatttc accttcgttc agcacgataa tctggtcagc atccataatg gttccgacac
     5161 gttgagccac aattagcacc gtcgcatttt ccgtcacttc cttcaaacga ctccgcaaaa
     5221 ttgcatccgt cttataatcc aaggctgaga aagaatcatc aaaaatgtaa atatccggct
     5281 gcttgacaat agcacgtgca atggacaaac gttgcttttg cccgcctgac aggttgcttc
     5341 cgccctcagc caagtgggtg tcgaattttt cttccttact ctcgataaac tccttagcct
     5401 gagccacatc tgtcgcatca tgaagttcag caagactagc atccatctta ccgtacttta
     5461 agttttccgc gatagttccg gtaaatagca gagctttctg tggaataaag ccaatcttgc
     5521 tgcggagtgc cttcagattg tagcgtctga catccacacc atccaccaag atacgaccaa
     5581 gtgtcacatc atagaaacgt ggaatcaagt tgaccaagga agacttacca gaacctgttg
     5641 aaccaataaa ggcaatcgtt tcaccaggct tagccttaaa tgaaatattg tggagaacag
     5701 gcgattccgt ttcaccagga taggcaaaag ttacattttc aaattccaaa taaccacgag
     5761 tatctgattt agtaatacct tcttcattct tagaaatgga aatgggcatg tccaagactt
     5821 tctgaattcg ttggctcgat accgacatac gtggatacat gttaaacaag ttagcaaaga
     5881 gcaagaaaga gaaaagcgcg tggaaactat attcgataaa ggctactaaa tcaccgattt
     5941 gtagactgcc tgttttcaag ggatccaaag caaaccagac aatggccaca atcatagcaa
     6001 tgataatctg gacaaagaga ggctctgtca atccagtcag attaaagagc tttttggaaa
     6061 tatcggtata ttcctcattg acctctgcaa aacgattttc ttggaactct tcacgtgtaa
     6121 aggcacgaat gacccgcaaa cccatgagat tttcacgaac atactggttg atagtatcca
     6181 atcgtttctg ttgtttttca gagagaggac gagtttttgt tgccacatag aaaataacaa
     6241 aaactagaaa cggcatggct actgctactg tccaagccaa gctcggactg gttaccaagg
     6301 tcataatgac agaagcaatc atcatcaagg gtgtaataat acctagtttc aagaccattt
     6361 cagagaattg catgagaaca aaagcatcat ttgtcatacg agcaactaga gaagaaacac
     6421 caatctgctc atattcatga tgagaatagt cctgaatttt ctcatacaga tcatttcgaa
     6481 tatctttgac gatattggta gtcaattttc cagctgcata ggcaagtgtc actcgaccaa
     6541 caatcccgag caggacaatc aatcccatca tccccgccca gaaatagagc tgactaacat
     6601 cgttctttgt aattccttgg tcaatcatcc tagccaaaaa agtcggcaag cctaagttta
     6661 ccactacaaa taagaaggcc cccaacaagt ctaaggcaat ccacttagga tattttttca
     6721 aataggtcca aatcagattc atcatttctt cctttctata tgttaaaaag gggctaggcc
     6781 cctttgtcaa cgttaaaagc caccaaacgg tgactttcta ttctttctcg tcgatcacta
     6841 aacgaacttt tttatcactt gttggaacag aatagacaat cgttctgatt gcagaaattg
     6901 tgcgtccttt tctaccaatg atacgtccaa tatcagattg atccaaatcc aaatgatatt
     6961 ccaagaattc aggtgtatca acaattttta ttgtcaagct atcaggctgt gaaatcaaag
     7021 gtttcacaat cgcaataatg agattttcaa tcatgtccat aagtgttctc gcttactgtt
     7081 cttacttaga gaatttttgt tcgtggaatt tcttcaatac gccagcgttt gaaagaaggc
     7141 tacgaactgt atcagatggt tgtgcacctt ttgccaacca ctcaagtaca cgctcttctt
     7201 taagagttac tgagttttca gccaaaagtg ggttgtaagt accaacagtt tcgatgaaac
     7261 gaccatcacg tggtgcacgt gagtctgcaa cgttgatacg gtagaaaggt ttctttttag
     7321 aacccatacg agtcaaacgg atttttactg ccatttttaa atgttctctt ttctttgtat
     7381 ttttattcgg tgaaatagca gagctattta gcacatgttc tattatacca cactttgaca
     7441 gggtgtcaag aaaaaaactt gacaggtttt aaaaaaattt attgttcgag catatctgct
     7501 agttctctca gttgacttgc tagatttgta cggtcaaaca gtaaattata gactccatct
     7561 tcactcaata ctccgcaagg aataccattg aaataaccct tattagataa atcaagatct
     7621 tccatatagg tctggctctg gtaataatct aataattgta aataagtcga ttgttcctgg
     7681 ttaagttgtg ccatattatc ggaaagggac tggttcagct ctagctgtct gttaaatatt
     7741 ttttccattt cctctacacg ttttcttgct tccataatat ttttcatcag tgtgtactgc
     7801 caccaccctt tttaatatcc tctttatccc tatattcaac aatcgctcct atagctactt
     7861 caactcctct tacaatatcg ttcaaagaca tggaagctag tccaggcttg tcgacaacct
     7921 gctctggcaa gaagggaata tggagaaagc ctgccttagt ctttggaaat tgtttctccg
     7981 ccaagtaaag agcttgatac atgagatgat tgcagacaaa ggtcccagca gtattagaaa
     8041 cagaagctgg aagcccaatc ctatgaatag cttcaaccat tgctttgatg ggtaaggtgg
     8101 agaaataagc aggctgacca tcttctcgaa tcgttctatc aatcggccgc tgcccttcat
     8161 tgtctggaat gcgtgcatca tcttggttaa tggctatacg ctcaggagtt aaatcaactc
     8221 gcccaccagc ctgaccaata catagtaccg catcaggtaa atgttctgcc attttctcct
     8281 ctaaaacatc cgcagccttg tcaaaaacag tcggaatctc tacaagaata atctctgcac
     8341 cagcaatggt cttaggtaga gatttgattg tttccaaggc tgggttaata gactcgccac
     8401 caaagggatc aaagcctgtt acgatgattt tcatacttcc tcctccaaaa taatttctcc
     8461 gtagtgaaat ttttgattgg gtctgacatg acgtttaaag ccaaattctt caaagacctg
     8521 atctcgaaaa tgagccttta taatcaattt cttcctagct actcgtttag cctcagatac
     8581 aatttcctcc gttaaacgac ttctgtcagc caaagtagac aagccagaaa gattttgtga
     8641 ttccttgatt tcttccgaaa acatgggatc aaagtaaatg acatcaacag acttatcttt
     8701 ttgctctttc aaataagtca aactatctgt ccaaattgtc tggatagatt tcatggctcg
     8761 attgacctct tgcagcccac tatcaaaatc ctgtaaacct cgactcacaa taaagtgcac
     8821 tagcttagaa ctttccaagg ctgttacccg atgaccggca cttgctaaca caatgctatc
     8881 agatcccaag ctcatagtac agtctattat tgattgtttt tcttttccaa gtaattctaa
     8941 aagcggatct cttccagact taatccgcaa catggcagta tctgggtgga aaaataaaac
     9001 ctgcccaccc ctctgctcta gtatcaattt atccttataa agaaccaaga cagcctcgta
     9061 ggtacctaac atttttccaa ccgactgctt ctttctttcc ttgtattcaa ttcccagttc
     9121 actggcaatc cgtctagccc tatagaccag cccctcatcc atcccaagac tagttgttac
     9181 aatcattttc atactcttca gtatatcaaa aaatcaggac gaactcaaag ctcatcctga
     9241 tagtatcttc tatatagcct gtgtcatgaa gctacggctc tccagcactt tgaccatagc
     9301 ttctgctgta tcgagggccg taaagagcgg aattcctcct tcaatagcag agcttcgaat
     9361 gacttgaccg tcaccatctg ccacccgttt tttcccaacc gtattgataa tagcctgaac
     9421 cttacctgcc cgaacaagac taggaatatc tggacgggaa tcatcgccta atttaccaac
     9481 agttgttaca gcaagtccat tttctgttag aaaggcggct gttcctgccg tagccagtag
     9541 gccataacca agttcataga aacgttgagc cagaccaagc acttcttcct tgtcttcatc
     9601 cgcaatggta aagacaacgt tgccaaactc agccaggtgc tgataacttg cctcgaaagc
     9661 cttataaagc gctttttcca gtgtcaagtc tgaccccata atctcaccgg ttgacttcat
     9721 ctctggtcct aacaaactgt caactttagc tagtttcgtg aacgagaaga ccggtgcctt
     9781 gacgtgcact tggttgctct caggataaag accgtcttgg tagccaagtt ccgcaaggct
     9841 ttgtcccaaa atcaatttgg ttgcaacctg agccattgga atgtctgtga ctttggacaa
     9901 gaatggcacc gtacgactgg cacgcggatt gacctcaatc acatagaccg tttcatcctt
     9961 gatgacaaac tggatattca tcataccgat acagtcgaga ccgattgcca agcgtttggt
    10021 gtagtctgca atggttgcct gcacttcttt ggacaaggtt tgtggcgggt aaacggccat
    10081 cgagtcacct gagtgaaccc cagcgcgctc gatatgctcc ataatacctg gaatcaagac
    10141 atccttacca tctgaaatag catccacttc acactcacga ccgacgatgt aagaatccac
    10201 caaaactggg tgctctggcg aagccttgac cgcagttctc atataagaac gtaagtcctc
    10261 ttcattttcg acaatctcca tagcacgacc gcccaagaca taagagggac gaacaagtac
    10321 cgggaaacca attttgcggg ccgcttcaag agcttcctct tcgttggttg cggtttgacc
    10381 tggtggctgt ggaattccta ggtctttcag ggctttttca aataaatccc tgtcttcagc
    10441 tctatccaaa tccgctactt gcgtacccaa gattggaata cctgctttgg ccaatggctc
    10501 tgccaaattg atagccgtct ggccaccgaa ctgaacgata accccttttg gtttctcaag
    10561 gtcgatcaca ttcaaaacat cttccaaggt taacggctcg aagtaaagct tgtccgaaac
    10621 cgaaaagtca gttgacacag tttcagggtt tgagttcata atgatggctt cgtagccagc
    10681 tgcctgaatg gccttgacag aatgtaccgt cgcatagtca aactccacac cctgtccgat
    10741 acgaattgga ccagaaccca gcaccaaaac agattctttc tccgaacgga tggactcatt
    10801 ttcccactca taagttgaat agaaataagg tgtagcactt tcaaactcag ccgcacaagt
    10861 atctaccatc ttgtaaacag ggacaatctt gtgttctgtc cgcagttgac gaatgtagga
    10921 ttctgtccgt ccccagagtt ccgcaatctt gcggtctgca aaaccgtatt tcttggcttt
    10981 tttcaataaa tcaatattat caaaattcgt tgccaattcc tgctcgattt ctaagatatg
    11041 aagcagctta tcaaggaaga aaagatcaat cttcgtcaat tgagctagtt cttctactgt
    11101 ataaccacgt cgaagggctt ctgaaagata gaataaacga tcatcctgtg ccttgacaat
    11161 cttttcaacc aattggtcat ctgtcacttg gctgagttca ggcatttcat tgtggtatac
    11221 cccgatttcc aaagaacggc aggccttgag cagactttct tcgatattac gcccaatggc
    11281 catgacctct ccagttgcct tcatctgcgt tccaaggcga cgctcccctt tttcaaactt
    11341 atcaaatggg aaacgtggaa tttttgccac aacgtagtca agagcaggct caaacatggc
    11401 ataggttgtt cctgtaacag ggttcaccat ttcatccaag gtcaagccaa ccgcaatttt
    11461 tgctgccaat ttggcaatag gataacctgt cgctttggat gccaaggcag aggaacgcga
    11521 tacacgagga ttgacttcga tgacatagta tttgaagcta tgagaatcca aagccaactg
    11581 cacattacac ccaccctcga ttttgagagc acgaataatg ctgagactgg catcacgcaa
    11641 catctggttt tcaatatccg aaagcgtctg cgttggggca aatacgatag aatctcctgt
    11701 atgaattccc acagggtcaa agttttccat gttacatacg accaaggcat tgtcagccgc
    11761 atcccgcatc acttcgtatt caatttcttt gaaaccagca atggaacgct caatcaaaca
    11821 ctgagtcact ggtgacaatt ttagaccatt ttctgctatt tcacgtagtt cttcttcatt
    11881 ggcacacata ccaccgccag taccacccag cgtgaaggct ggtcgaacaa tgacaggata
    11941 gccaatttca gcagcaaatt ccaaagcctc atcaactgtt gtcacaatcg ttgactcagg
    12001 aataggctga ttgagatctt ccatgagttg tttgaagagg tcgcggtcct ctgcctgatc
    12061 aattgcagac agtttagttc ccaacaactc aactccaagc tcatctaaaa taccagcctt
    12121 agacaattcc atggccatgt tgagaccagt ctgaccacca agagttggca agagagcatc
    12181 tggacgctcc ttccgtaaaa tccgtgtgac aaattcaagc gtaattggct caatataaac
    12241 cttgtctgct atttccttgt ctgtcataat ggtcgcaggg tttgaattga ccaagaccac
    12301 actgtaacct tcttccttca aggctaaaca agcctgagtc ccagcataat caaactccgc
    12361 agcctgacca atcacaatcg gaccagaccc aatcaccatt attttcttaa tatccgtacg
    12421 ttttggcata atgtatgata caaagcccgt taagaggaca aagcgaaaat aggaaacacg
    12481 acactgatgc ttcaagcatc aagacgacat ttatcttttt cgcacagtcc ttagggcgtg
    12541 ttcgaatagg aaactcgaac ctgtatctct cctttctatt ttctatctct ccggatagaa
    12601 ttaaataatt gtccgacgtg agaaaatgga ttcgacatta atccattttc gagctagtgc
    12661 aagggatagc ctgacctcca tagaggcagg cgtagggaaa tgagttgctc gcaaccattg
    12721 atctgtccct tgcctttcta ttcgtcgcct ctctgagcga cattaaataa gataagtccg
    12781 acgtttagaa tcccaattcg ataataattg aaattctaaa ctagtgtcag agttagcaaa
    12841 ggtcccattg gactttgcgt aggaagacac atgcgttaac atgaagtccg acttgctctg
    12901 accgacgcag taaagtttag cacgatatta gttaaacctt actgcccttt tttaaacgaa
    12961 tccatcaatt ccataaattc gtcaaacaaa tagctggcat catgtggacc aggtgcagca
    13021 tctgggtgga attgaacaga gaaacctgga tagtacttgt ggcgtacccc ttctactgat
    13081 ttgtcattga tttcttcatg agtaatcatt aaacattcag gtaaatcttc acgagccact
    13141 gcataaccgt ggttctgact ggtaaagtcc actcgacctg tcgcaatttc acgaacagca
    13201 tggttgaaac cacggtgacc aaacttcatt ttataagtcg ttgccccatt tgctttagca
    13261 aagagttggt gccccataca gataccaaaa attggaattt ttccgagaat gccccgaatc
    13321 atgtccaaag cttctggaac atcatctggg ttaccaggac cgtttgaaag catgacaccg
    13381 tctggattga gggcaagaat ttcttcagca gtcgtatcat aagggacaac tgtcacattg
    13441 caatcacgct tggctaactc tctaagaata gaatgtttca atccaaaatc aaccaaaaca
    13501 acactacgtc caactccagg tgcaggatag gctgtttttg tcgatacttg ctggatgttg
    13561 ttggttggca aaacagtcgc tctcaactgg tctgtcaaat gctcaacgga atcaccaaca
    13621 tttgccaatg tcgccttcat ggtcccgtgc tgacgaataa ttttagttag ggcgcgtgtg
    13681 tcaattccag aaattgctgg aattttctta gcctttaaaa attcatccaa agtcatctgg
    13741 ttacgccaat tacttgctcg acgagcccac tcgctaacaa ccactccctt acaagtcggt
    13801 ttgatcgact cgaaatcatc acggttaatc ccgtaatttc ctaccaaggg ataggtaaag
    13861 gttaaaatct gaccattata agactggtcc gtgatggatt cctgataacc tgtcatccca
    13921 gttgagaaaa ccaactcacc agtcacatca atatcagcac cgaaggcctc cccctcaaaa
    13981 atagtgccat tttccaaaat taaacgtctt tttgacataa tctatgatac aaagcccgtt
    14041 aaaaggacaa agtgaaaata ggaaattcgg cgccgacgct ataggcgtca agtcgatatt
    14101 tatctttttc acacagtcct tagggcgtgt tcgaatataa aaatcaaatc ttactcgaac
    14161 ctgtaccttc tcctttctat tcgtcgcctc tctgggcgac attaaataag ataagtctaa
    14221 caaatttcta actgctcagt tctaaggtat ctgaacagct tacttttaag ccttgccatt
    14281 gacaattgct tccaaaatcg ccatccggac aaatacacca ttctgcattt gacggacaat
    14341 acgtgatttg ggtgcttcaa ctagagaatc atcaatctca acatcacggt taacaggagc
    14401 cggatgcata acaattgcag tatctttcaa gcgtttgtag cgctcctctg ttaaaccatg
    14461 caaacggtta taagtttctt tagaaaagcc accatcacca tcatgacgct cgtgttgcac
    14521 acggagcaac atcagtacat ctaccgtttc cacaatatca tcaatattca aatgctgacc
    14581 atagacatca aattcctctg cgtaccattc ctccggtcca gcaaagaaaa tctcagctcc
    14641 cagacgtttc aaaatttgca tattggactt agcaacccgt gagtgtgtaa tatcacctgc
    14701 aatggcaatc ttcaagcctt caaaagttcc aaattcttca taaatagtca tcaaatcaag
    14761 tagggattgg cttgggtgtt gacctgaacc atcaccacca ttgacaattg aagtctgaat
    14821 ggtaggacta tcaatcaatt gcttgtaata atccacttcc gagtgacgaa tcacgcagat
    14881 gtctacccca agtgctgaca tggtcaaaat ggtatcatac aaggtttcac ccttattgac
    14941 tgagctggta cgagcatcaa agtcaatcat tcccatatcc aaacgaagct ctgccatttc
    15001 aaaagactta tgcgtccgag ttgaatcctc aaagaaaagg ttagaagcat agtacttccg
    15061 atccaactct accttttcac cgcgtttaaa agcaatacca cgttgaatga gtcccaatac
    15121 ttcttcattt gagagggtct ccattgtaac caagtgtttg agtgaaactt taccatttgt
    15181 aattgtcatg atgataccta cctttatgat ctagatgtag aatgagaatt tgtaaacgaa
    15241 gcgactatgc accttcaacc agaaggacag cgtcttgacc atcgatttca gccatgtgca
    15301 caatgatttc ttcagaacgg ctagttggga tattcttacc aacataatct gctcgaatag
    15361 gcaactccct gtgtccacga tccacaagtg ccgctaaact aacacgagct ggacggccca
    15421 aggacactat gttgtcaata gcagcacgaa tagttcgtcc tgtataaagc acatcatcta
    15481 ctaatattac atcacgatca ttgacattgg cagtcatatt tgtcgtatca tcttctacct
    15541 tcatgtcatc tcgaaacggc ttggtatcca actctcccaa cggaacgtcg ataccctcta
    15601 attgcttaag tctctcctga atcctctttg caatgaaaac accccgtgtt ttaatccctg
    15661 caaggacaat attatccaaa ttcttattcc gttcgataat ttcataagtg atgcgggtga
    15721 ttgcccgttt catagtcata tcgtcaacga tttcttttgt cttcataaga cctccaaaag
    15781 tatagaaaaa gtctccttat acaaggagac tatgaaaata taggctagtt agaaccaacc
    15841 tgcttaacat ccgactcctt gtctgcctct ctggacaggt tttaaaggat ttattaagca
    15901 gagtatagca taaaatctca gccttgacaa tttttttctg aaaaaatata gaaattatcg
    15961 gattttactg agaatttttt ccaaaagaca at
//
LOCUS       DASFSW010000034        15956 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.034, whole genome shotgun sequence.
ACCESSION   DASFSW010000034 DASFSW010000000
VERSION     DASFSW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15956
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.034"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(11..262)
                     /locus_tag="U1099_000827"
     CDS             complement(11..262)
                     /locus_tag="U1099_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867161.1"
                     /translation="MFKEFNKEFYRSFNFYLMILLLLGSIGEWLDKGPTLWLGLLLFA
                     SVGTARSAFWSKEWMLRANWQIYLCIAFLLVGAWVEFFV"
     gene            complement(272..526)
                     /locus_tag="U1099_000828"
     CDS             complement(272..526)
                     /locus_tag="U1099_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867162.1"
                     /translation="MSKKFNKAFYRTWEFYLSLACILQVISAAVNGPFWFIHFLKLGL
                     GLYAGYLAFFKSRSWIKKTWQIYLTIGLIASWTIISFFVL"
     gene            complement(540..806)
                     /locus_tag="U1099_000829"
     CDS             complement(540..806)
                     /locus_tag="U1099_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867163.1"
                     /translation="MKFQFNKHFYRYSAFYSMIFWTCFFLSDILLDPSQTPAWYSLLA
                     SMIILIHAIRTFFFKEEKELTRMGYIFYIGLIVVIIYLKFILGK"
     gene            1009..2100
                     /locus_tag="U1099_000830"
     CDS             1009..2100
                     /locus_tag="U1099_000830"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEM4867164.1"
                     /translation="MTDYKIDTILAHTGINSDERTGALISPIHLSTTYQHPEFGQSTG
                     YDYTRTKNPTRASLETTLAAIEKADYALATSSGMAALVLLFNGFPVGSQVVAARDLYG
                     GSFRWFNEQESIGRFQFTYANTEEELIAAITEETDYVYLETPTNPLMVEFDIAKVSAI
                     AHAKGAKVIVDNTFYSPIYQNPLVLGADVVLHSATKYLSGHNDVLAGALMTNDQALYD
                     KLFYDQNTSGPTLSPLDSYLLMRGLKTLSLRMERATQNAQKIVAYLEKSPAVKQVYYT
                     GKGGMISVKVVDESRIPHILNTLKVFTFAESLGGVESLITYPATQTHADIPAETRHSY
                     GLTDDLLRLSIGIEDAEDLIVDLKTALEG"
     gene            2104..3264
                     /locus_tag="U1099_000831"
     CDS             2104..3264
                     /locus_tag="U1099_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM4867165.1"
                     /translation="MTQYNFIDSPNRLSQHAEKWRKVETDKELLPLWIADMDFEPLPE
                     IRQVIRDYADHHVFGYPYASDSLYQSIIDWEDRQHGYKINRESILLIEGVVPALSVAI
                     QSLTEEGDAVLINTPVYPPFARTVKLNNRQLVTNSLVDVDGVFRIDFDQLEKDIVENQ
                     VKLYIFCNPHNPGGRVWTREEVLAIGRLCQKHGVILVSDEIHQDLTLYGHKHYSFNTV
                     DESFKEFSIILSSATKTFNIAGTKNSFAIIENPSIRKTFAKRQLINNQHEIPTIGLLT
                     TEAAFTYGDEWLKELKVILERNIDFVEEYLITHTRIKVMKPQGTYLIWLDFSAYELEH
                     AELFDILHCKAKLILNDGLTFGKEGKYHARLNVAVPFNMIEEACQRLGKVFG"
     gene            complement(3296..4135)
                     /locus_tag="U1099_000832"
     CDS             complement(3296..4135)
                     /locus_tag="U1099_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM4867166.1"
                     /translation="MNILNIYNELDSHNFDLNVDHYGAEQCDKGYSFGPTIRDNFVLH
                     FITKGKGKILVDGKTSYLAAGDLFILPKDVSIFYQADDIDPWTYIWVGFSGSRAKDLL
                     NQSQLLENYYLHSNLESPILGYMHQINHVQMHPIPSITELVLIGYLNQLLAALIEEFP
                     SETLIKPETQTKHYVQQAIKMIHNHYAHPIKVSEIADFLALSRSYLYKIFKQETGYSI
                     KDYILQVKMNRSCQLLEDASRSITEIAYSVGYQDPLTFSAAFKNYFHMSPTDFRNTQK
                     NKD"
     gene            4241..6415
                     /locus_tag="U1099_000833"
     CDS             4241..6415
                     /locus_tag="U1099_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEM4867167.1"
                     /translation="MNVIEIYNEKQIFHLKTREFSYIIQVLETGDLVHRYFGKKIEKF
                     SDGNKITYLDRSFSPSPITGDRTYSLDVLPLEYSSNGLGDFRTSALDVRNEFGVTLDL
                     KYKEYRLYKGKKELRGLPASFGNQEEVESLEIDLYDQLTDVTVTLQYSVFEEASYLAR
                     SATIQTGKYPCKLEKVLSATLDFPHQDFIVHSLAGRYAYEKEWTQTPLTKGQYSIGSI
                     RGASSHSRTPFLALASPDASEDKGDVYAAHLVYSGNFTAFVETTAMETSRLGLGLESH
                     YFSWQLDKDDRFQTPEVLLSYTDKGFTGMTQNSHHFITKHLIRSSFVNKPRPILINNW
                     EATYFEFTEEKILQLAQVASRAGIELFVLDDGWFGKRNNDESSLGDWKVNLDKLPNGL
                     NGLADRINELGMKFGLWFEPEMISIDSDLYREHPDWAIRTEGRLPIYSREQLVLDLTK
                     QEVCDYIIDSVSSILESANISYVKWDMNRNITNIPEGLANDQRFEFHHRYMLGLYRVL
                     DHLTKRFPDILFESCAGGGGRNDLGIMYYMPQAWASDDTDAIERLSIQEGTSLIYPPS
                     SIGAHVSAVPNHQVGRITPLATRGNVAMMGGAFGYELDLTKLSEKELDEISQQIETYH
                     SIRETIQFGQLYRLKKTSNTWAANYVGQDKNQVVFTFVKILAKPEAPLLYVRLKGLDP
                     AALYECPQLGETFYGDELMNIGLTMPHVQNDYFSVQYIFNKI"
     gene            6429..7664
                     /locus_tag="U1099_000834"
     CDS             6429..7664
                     /locus_tag="U1099_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM4867168.1"
                     /translation="MKMKTFLKCASVCAFASFLVACGNASSSDKVEIEYFSQKPEMQA
                     TLQEIIDDFEKENPTIDVKFSNVPDAGTVLKTRMANNEAPDVINIYPQNADFKAYAAD
                     GRFLEIGDDAGLSHLKDGAVTPYLVNEKNYTLPLTANAYGIYYNKDKFKELGLEVPTT
                     YAEFVALVDKIKADGSAAPFALSLNDAWSLNGYHQLAWVTVAGGFDGAEDILIRSAKG
                     AIQDDATTKAVLERLQLLKDNGQKGATGALYADAVAAFAAGDALMLPQGTWAATAVNQ
                     QEPEFEYGMFTFPGDKEGGDYTIGAADLALSISADTEHPEESKKFLEYLSRPEVIQKY
                     YDVDGSSTSVEGVDTEGKFEETAGVTQYAFTDKHVVWLQSEWESEDEFWNITVEMVKN
                     PNSAELVKKLNAFFDPMKK"
     gene            7801..8673
                     /locus_tag="U1099_000835"
     CDS             7801..8673
                     /locus_tag="U1099_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM4867169.1"
                     /translation="MNQKGFIAKYWPYLFVAIPISLQLIFFFYPLFTGIFYSLTDWNG
                     LTSDFSLIGVQNYLDILKNPDFYTSMTFTIIFTIGLVIGEIVIGIWLASLLNRKIKAV
                     GFFRAWYFFPAVLSTVTLGLIFVQLFNYGFTQIGEILHIDWLMENLLVQENTVIPAVL
                     FVALWQGLAMPVIIFLSGLQSIPEDVKEAAAIDGATRSQQFFNIELPFLLPSISMVFI
                     LAMKSGLTAFDLIFALTSGGPDGKTESLGLLVYNYAFVDNKFSYANALAVVLFIFIIV
                     ISLIQMRISKKFEI"
     gene            8684..9514
                     /locus_tag="U1099_000836"
     CDS             8684..9514
                     /locus_tag="U1099_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM4867170.1"
                     /translation="MNTQKQKNWWVYLVLFSGILFMFIPLLVTIISSFKPTKEITSNF
                     FGLPENFTLNNYERLFNDGIVQYFGNSALITIVAVGLILLVIPMAAFAVARQMKRQTV
                     FNFIYFFLIIGIFVPFQVIMLPMTKLMSSLGLNNIVGLIILYLTYAVPQALFLYVGYI
                     KTIVPEEMDEAAAIDGCDKFTMYWKIIFPLMKPMHATVLIINALWVWNDFLLPLLVLN
                     RDQSMWTLPLFQYNYQGMYFSDYGPSFASYVVGIIPILLVYLVFQKHIISGMTSGSVK
                     "
     gene            9583..11031
                     /gene="gtfA"
                     /locus_tag="U1099_000837"
     CDS             9583..11031
                     /gene="gtfA"
                     /locus_tag="U1099_000837"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEM4867171.1"
                     /translation="MKIKNQAMLITYSDSLGKNLKDLKKVLEGPLKDVVGGIHILPFF
                     PSSGDRGFAPMDYTKVDPAFGDWSDIEALSKDYYLMFDFMINHISAKSPYFLDFLEKK
                     DESAYADLFIRYKNFWPNGEPTQEDVDLIYKRKPRAPYRIATFADGSEEKVWCTFDEQ
                     QIDLDVTTETTRRFIQENLEQLAEHGASIIRLDAFAYANKKLGTNCFFVEPDIWEMLH
                     FPRQLLAPKDVEILPEIHEHYTIQQKIAEQDYYVYDFALPMLVLHALYSGHVNRLVHW
                     MEICPRKQFTTLDTHDGIGVVDVKDLLTDEETEETREALYAQGANVKKIYSTEAYNNL
                     DIYQINCTYYSALGNNDQAYLLARVLQCFAPGIPQIYYVGLLAGENDIELLESSKEGR
                     NINRHYYDLEEIEQEVQRPVVQSLFKLLKFRNTSPAFDGEFSVKMLDETSMEIFWNNQ
                     DAGVSARLTANLKEKSFEIVEIEEGKITTIEL"
     gene            11104..12720
                     /locus_tag="U1099_000838"
     CDS             11104..12720
                     /locus_tag="U1099_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEM4867172.1"
                     /translation="MSEIQKTDWWKKSVIYQIYPRSFQDSNGDGIGDIRGIISRLDYL
                     HELGIDAIWLSPVYQSPMDDNGYDISDYQGIAPEFGTMEDMEELIAEGHKRNIKIIMD
                     LVLNHTSDEHFWFQEALKGPDNPYYDYYVWADEPNELRSTFSGSAWEYVPHLNKYYLH
                     QFSVKQPDLNWKNPALKQEIWDMINFWIEKGVGGFRLDVIDLIGKEPEKLITADGPTL
                     HPLIQELNEKTFGAYDLVTVGETWSANPENAQLYSHPDRKEFSMIFQFEHVGLDQQEG
                     KEKWDLAPLDPARLHKVLSKWQTELVGKGWNSLFWNNHDLPRAISRFGDDRPAFRELS
                     GKMLAIYLHFMSGTPYIYQGEEIGMINTPITDISQADDIETRRMYAERIENGFTKEEL
                     ITSINAKGRDNARRPMQWTAEEGAGFSTGQAWLALGDTVKDINVEKALADKQSLFYTY
                     QKLIALRKEYPCMSEGKYEVMETGNDLVMAYRKYDEQDDFIILVNFTSEEQAYEFSLE
                     DYSVFMHNYDNKVIFDKSVLRPYEAIVFKK"
     gene            12803..13432
                     /gene="upp"
                     /locus_tag="U1099_000839"
     CDS             12803..13432
                     /gene="upp"
                     /locus_tag="U1099_000839"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEM4867173.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTSTSTKDFRELVNEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQIAGKKLAIVPILRAGVGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRHIFVVDPMLATGGSAILAVDSLKKRGASNIKFVAL
                     VSAPEGVKALQEAHPDIDIYTAALDEKLNEKGYIVPGLGDAGDRLFGTK"
     gene            13549..14139
                     /locus_tag="U1099_000840"
     CDS             13549..14139
                     /locus_tag="U1099_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEM4867174.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSIIA
                     QLLFLDAQDPTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGTAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNKLEKILADNSGK
                     TVKQIHKDAERDYWMSAEETLAYGFIDQIMDNTKVK"
     gene            14266..14538
                     /locus_tag="U1099_000841"
     CDS             14266..14538
                     /locus_tag="U1099_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEM4867175.1"
                     /translation="MFEKKNRTCLTVYLHYNRDARKLSQYGDIVYHSKRLRYVLVYMD
                     QELVEATILKLKKERFVKKVVPSYIKELDQNFVGNLWRDEEPSVVG"
     gene            14669..15838
                     /locus_tag="U1099_000842"
     CDS             14669..15838
                     /locus_tag="U1099_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4867176.1"
                     /translation="MKTRKFAVALATFASAALLAACGNVSTTNTSAAGSSVGDTFKIG
                     YNLELSGAVSSYGQTEEKGANLAVKEINAAGGIDGKKIEVITKDNKSETAEAATVATS
                     LASEGANVVIGPATSGASAASIPALTSAGVPMITPSGTQTNLVVNDKGEVQEYFFRTT
                     FTDGYQGQIMAKYATENLSAKKVVLYFDNSSDYGKGVAEAFKKAYTGEIVSEITFASG
                     DKDFQAALTKLKDKEFDAIIMPGYYNETGTIVKQARGLGIDKPILGSDGFDSPQFTEL
                     ATASAASNVYYLSGFVTSASEKAKAFYDAYVKEYNEEPSMFSALAYDSVYMAAEAAKG
                     AADSAAVKANLAALKDFKGVTGTMSVDKEHNVIKSVYVVSLTNGEESSVDTISID"
BASE COUNT         4927 a         2944 c         3336 g         4749 t
ORIGIN      
        1 ctcccaactc ttaaacaaaa aattctaccc aagccccaac tagcaagaat gcgatgcaga
       61 gatagatttg ccaatttgcc ctcaacatcc attctttaga ccaaaaggca ctgcgggctg
      121 taccgacaga agcaaagagc aagagcccaa gccagagggt aggccccttg tccaaccatt
      181 ctccaatgct accaagcaac aacaatatca tcaaataaaa gttaaaactc cgataaaatt
      241 ctttattaaa ttctttaaac atataaaatc ctcacagtac aaagaaggaa atgattgtcc
      301 aactagcaat caatccaatc gtcagataaa tttgccatgt ttttttaatc cagctacgtg
      361 acttaaagaa agctagataa ccagcataaa gtccgagtcc aagcttgaga aaatggataa
      421 accaaaacgg tccatttaca gcagcagaaa tcacttgtag gatacaggca agagacaagt
      481 aaaattccca ggtccgatag aatgctttat tgaatttttt agacatagta agctcctttc
      541 tatttaccca aaataaattt cagatagata atcaccacga tcaatccgat atagaagatg
      601 tagcccatgc gagtgagttc tttttcttcc ttgaagaaaa aggttcgaat ggcatgaatt
      661 aaaataatca tagaggccaa taggctatac catgcaggcg tttgggaagg atctagcaat
      721 atgtctgaca gaaaaaaaca agtccaaaaa atcatagaat agaaggcgct ataacgatag
      781 aaatgcttgt taaactggaa tttcatgaca aatcacctct ctttctttta ttatatcaaa
      841 taatgtatag gtttaaaagt atttattggt aaatccagta tgattattgt tataaagaaa
      901 aactatagca aagcttgcta tttttctata agtatttttg gatatttttc acaccaaatc
      961 tcctatttca tgctagacta tattgtaagt tagattggag tagatgttat gacagattat
     1021 aagattgata cgattttagc gcatacgggc atcaatagcg atgagagaac aggtgccttg
     1081 atttccccta tccacctttc gacgacctat caacaccctg agtttggtca atcgacaggt
     1141 tatgactaca cgagaacaaa aaatccgaca cgtgccagtc tagagacgac tttggcagct
     1201 attgaaaagg ccgactatgc cctagcaacg agctcgggaa tggctgctct ggtcttactt
     1261 tttaatggtt ttcctgtagg gagtcaagtg gtagcggcgc gtgatttgta cggtggttcc
     1321 ttccgctggt tcaatgagca agagagtatc ggtagatttc aatttaccta tgcaaacacc
     1381 gaggaggagc tgattgcagc catcaccgaa gagacagact atgtttatct ggagacaccg
     1441 actaacccac tcatggtgga gtttgacatc gctaaggttt cggctatcgc ccatgccaaa
     1501 ggtgctaagg tgattgtcga taacactttt tacagcccta tctaccaaaa tccacttgtt
     1561 ctaggagcgg atgttgtcct acattcagca accaagtacc tatctgggca taatgatgta
     1621 ctagctggcg ctttgatgac caatgatcaa gccctatatg ataagctctt ttatgaccaa
     1681 aatacatcgg gtccaaccct atctccactg gattcttact tactcatgcg tggactcaaa
     1741 accctctcgc ttcgaatgga gcgggcgaca caaaatgctc agaaaattgt tgcctatttg
     1801 gagaagagtc cagcagtcaa gcaagtttac tacacaggaa aaggtgggat gatttcggtt
     1861 aaggtggtag atgaaagtcg gattccccat attctcaaca ccctgaaagt ctttaccttt
     1921 gctgaaagtc tgggtggagt agagagtctg attacctatc cagccactca gacccacgca
     1981 gacattccag ctgaaacccg tcattcttac ggtttgacag atgatttgct tcgtctgtct
     2041 attggtatag aggatgcgga agacttgatt gtagatttaa aaacagcctt ggagggataa
     2101 gatatgacac agtataattt tatagatagt ccgaatcgtc tatcccagca tgcggaaaaa
     2161 tggagaaaag tagagacgga taaagaactc ttacccttgt ggatagcaga tatggacttt
     2221 gagcctctac cagaaattcg ccaagttatc cgtgactacg cagatcatca cgtttttggt
     2281 tatccctatg ctagtgacag cctctatcag tctattattg actgggaaga tagacagcat
     2341 ggctacaaga ttaatagaga gtccatcctg ctcatcgaag gagttgttcc tgccttatct
     2401 gtggctattc aatctttgac agaagaaggg gatgcagtcc taattaatac acctgtctat
     2461 ccaccctttg cgcggactgt aaaattaaac aatcgtcagc tagtcaccaa ttcactagtt
     2521 gatgtggatg gtgtttttag gattgatttt gaccagcttg aaaaggacat tgtagagaat
     2581 caagtgaaac tctatatttt ctgtaatccc cacaacccag gtggtagagt ttggacaaga
     2641 gaggaagtgt tggctattgg acgtctttgt cagaaacatg gtgttatcct tgtctcagat
     2701 gaaatacatc aagatttgac cttgtatggt cacaagcatt attcctttaa cacggtggat
     2761 gaaagtttta aagagttttc aatcatctta tcatctgcga ccaagacctt caatatcgct
     2821 ggtacaaaaa atagctttgc aatcattgaa aatccaagca tccgcaagac tttcgctaag
     2881 cgtcaattga taaataatca gcacgagatt ccaacgattg gcttgttaac cacagaagct
     2941 gctttcactt atggagatga gtggttgaag gagttgaagg ttattttgga gagaaatata
     3001 gactttgttg aggagtattt gataacccat acaaggatca aggtcatgaa gccccaggga
     3061 acctacctca tctggcttga tttttctgct tatgagttag agcatgcgga gctctttgat
     3121 attcttcatt gtaaagcgaa gttgattttg aatgatggtt tgacctttgg taaggaaggt
     3181 aagtatcatg cacgtctgaa tgtagcagta ccattcaaca tgattgaaga agcttgtcag
     3241 cgacttggta aggtttttgg ataaagaaat aaggactagg tgtgagtaaa atcacctaat
     3301 ccttattttt ttgtgtattt ctaaaatctg ttgggctcat atggaaatag tttttgaagg
     3361 ccgcactgaa ggtgagaggg tcttggtatc cgacagaata ggcgatttca gtgatactcc
     3421 gagaagcatc ttctaatagt tgacaagaac gattcatttt tacttgtaaa atataatctt
     3481 tgatagaata gccagtttcc tgcttaaaaa ttttatagag atagcttcgg cttaaagcta
     3541 gaaaatctgc tatttcagaa actttaattg gatgggcata gtggttatgg atcatcttga
     3601 tggcttgctg aacatagtgc ttggtttgtg tttcaggctt aattaatgtt tcactgggaa
     3661 attcctcgat gagtgctgct aagagttggt tgaggtagcc aattaagacc aattctgtga
     3721 tagaggggat gggatgcatc tgaacatggt taatctgatg catgtagcct agaattggtg
     3781 actctagatt ggagtggagg tagtagtttt ctagcagttg actttgatta agtaggtctt
     3841 ttgctcgtga accactaaaa ccaacccata tataggtcca cgggtctata tcatcagctt
     3901 gatagaaaat ggaaacatcc tttggaagaa taaacaaatc gcctgcagca aggtagcttg
     3961 tttttccgtc aacaagaatt ttcccttttc cttttgtaat aaaatgcagg acaaaattat
     4021 cacggatagt aggaccgaag gaatagccct tgtcacattg ttcggcaccg taatggtcta
     4081 catttaggtc aaagttgtga ctatcaagct cgttatagat attcagaata ttcatttcaa
     4141 tcctccttag gaaacattat aacatgtttt aaatacaaaa gaccattaaa taaagcgttt
     4201 tcattttgta aaataatagc ataaagaaga gatgggagag atgaatgtga ttgagattta
     4261 taatgaaaaa cagatttttc acctgaaaac aagagaattc tcttatatca tccaagtgtt
     4321 ggaaacaggc gatctggttc atcgttattt tgggaaaaaa atcgaaaaat ttagcgatgg
     4381 aaacaaaata acatatttag atcgatcgtt ttctccgagt ccaattactg gggataggac
     4441 atactcattg gatgtcctcc cactcgaata ttccagcaat ggtctgggag attttagaac
     4501 ctcagcatta gatgttcgga acgaatttgg agtcactctt gacctaaaat acaaagagta
     4561 tcgattatac aaggggaaaa aagaactgag gggattacca gcaagttttg gaaatcaaga
     4621 agaggtagag agtcttgaaa ttgatcttta cgatcagttg acagatgtta ccgtaacact
     4681 tcagtattct gtttttgaag aagcttccta tctcgcacgg tcagctacaa ttcaaactgg
     4741 caaatatcca tgcaaactag agaaggtctt gtctgcaacg cttgattttc cacatcaaga
     4801 tttcattgtt cacagcttgg caggacgtta tgcttatgaa aaagagtgga cacagacccc
     4861 attgacaaaa ggtcagtatt cgattggtag cattcgaggt gcttcaagtc actcaagaac
     4921 acccttctta gcactagcct cacccgatgc aagtgaggac aagggcgatg tttatgcagc
     4981 ccaccttgtc tatagcggca actttacagc atttgtcgaa acgacagcca tggaaaccag
     5041 tcgattggga ctaggattgg aaagtcacta tttctcatgg caattggata aggatgaccg
     5101 gtttcaaact ccagaagttc tcttatcata cactgataaa ggatttactg gtatgacaca
     5161 aaatagtcat cactttatca caaaacacct cattcgatca tcgtttgtaa acaaaccaag
     5221 gcccatttta atcaacaact gggaagcaac ctattttgaa tttacagaag aaaagatttt
     5281 acagttagct caggttgcaa gtcgagctgg tattgaattg tttgttctgg atgatggttg
     5341 gtttggtaaa cgaaacaatg atgaaagttc attgggcgat tggaaagtca atttggataa
     5401 gttgccaaat ggtttgaatg gtttagcaga ccgaataaat gagcttggca tgaaatttgg
     5461 actgtggttt gaaccagaga tgatttctat tgatagcgat ctttatcgtg agcatcctga
     5521 ttgggcaatt cgcacagaag gacgcttacc aatctacagc cgagaacaat tagttttgga
     5581 tttgaccaag caggaagtct gtgactacat cattgatagc gtctcttcca tattagaatc
     5641 agccaatatt tcttatgtaa aatgggatat gaaccgtaat atcaccaata tccctgaagg
     5701 tttagcaaat gaccagcgct ttgagttcca ccatcgttac atgctaggtc tctaccgtgt
     5761 attagaccac ctcacaaagc ggttcccgga tattcttttc gaatcctgtg caggcggtgg
     5821 aggtcgtaat gatttgggaa tcatgtatta catgccacaa gcttgggcaa gtgatgatac
     5881 agatgcgatt gagcgcttat ctattcaaga aggtactagt ttgatttatc ccccttcctc
     5941 aattggtgca catgtttctg ccgttccgaa ccatcaggtt ggtcggatca cacctcttgc
     6001 tacacgaggc aatgtggcaa tgatgggagg agcatttggt tacgagctgg atttaacgaa
     6061 actttcggaa aaggaattgg atgaaatcag tcagcaaatc gaaacctatc actccattcg
     6121 tgaaactatc caattcggtc agctctaccg tctgaaaaag acgagcaata cctgggctgc
     6181 caattatgtt ggtcaagata agaatcaagt ggtctttaca tttgtaaaaa ttcttgccaa
     6241 gccagaagcg cctttgcttt atgttcggtt gaaggggcta gacccagctg ccctatatga
     6301 atgccctcaa ttaggggaaa ctttctacgg ggatgaattg atgaacattg gactcaccat
     6361 gccccatgtt caaaatgatt attttagtgt acaatatatt tttaacaaaa tctaggagga
     6421 ttttatatat gaaaatgaaa acatttttaa aatgtgcgtc agtttgtgcc tttgccagct
     6481 tcttggttgc ttgtgggaat gcaagtagta gcgataaggt agaaatagaa tatttctcac
     6541 aaaaacctga aatgcaggct actcttcagg aaattattga tgatttcgaa aaagaaaatc
     6601 ctacgattga tgttaaattt tcaaatgtac cagatgcagg aactgttctg aaaacccgta
     6661 tggcaaataa cgaagcgcca gatgtcatca acatttatcc acaaaatgcg gacttcaaag
     6721 catacgcagc ggatggtcgt ttcctagaaa ttggagatga tgcaggtctc agtcatctaa
     6781 aagatggtgc tgtaacgcca tatcttgtta atgaaaaaaa ttacaccctt cctttgacag
     6841 caaatgccta tggtatttac tataataagg ataagttcaa agaattgggg ctcgaagttc
     6901 caactaccta tgcagaattt gtggctttgg tagacaaaat caaggctgat ggcagtgcag
     6961 caccttttgc cctttcattg aacgatgctt ggtcattgaa cggctaccat cagttagctt
     7021 gggtaactgt tgcgggtgga tttgatggtg cagaagatat tctgattcgt agtgccaaag
     7081 gcgcaattca agatgatgcg acaacaaaag cagttttaga acgcttgcaa ttgttgaaag
     7141 acaatggaca aaaaggggca acaggcgcgc tctatgcaga tgcggtggca gcctttgcag
     7201 cgggtgatgc tctcatgctt ccacaaggta catgggcagc tacagctgta aaccaacaag
     7261 aaccagaatt tgaatatggt atgtttacat tccctggcga taaagaaggt ggcgactata
     7321 ccatcggtgc agctgacctt gctctatcta tttcagcaga tacagagcac ccagaagagt
     7381 ctaaaaaatt cctagaatac ctttctcgtc cagaagttat ccagaaatac tatgatgtag
     7441 atggctcgtc aacttcagtt gaaggtgtag atacagaagg taagtttgaa gaaactgctg
     7501 gagttacaca atatgccttc actgataaac acgtagtttg gttgcaatct gaatgggaat
     7561 cagaagatga gttctggaac attactgttg aaatggttaa aaatccaaac tcagcagaat
     7621 tagtgaaaaa actgaacgca ttctttgacc caatgaaaaa ataattcaag aaactataag
     7681 gagggctaga acagcctagc tcttctttat agttgcttag ggtcagtttg aataccccct
     7741 aaccactcca ttcaaactga ccctaagtaa ctatacattt tagaaaaaag gagtaccatg
     7801 atgaatcaaa aaggttttat tgccaaatat tggccctatc tatttgttgc cataccaatc
     7861 agtctacagt taatcttttt cttctatccc ctgtttacag gtatatttta cagtttgaca
     7921 gactggaatg gattgacatc tgatttcagc ttgattggcg ttcaaaacta tctagatatc
     7981 ttgaagaatc ctgattttta tacgtctatg acttttacca ttatcttcac cattggtttg
     8041 gtcatcggag aaattgttat agggatttgg ttggctagtc tcctcaatcg taagattaaa
     8101 gctgttggtt tctttagagc ctggtatttc ttccctgcgg ttctttcaac cgttacttta
     8161 ggcttgattt ttgttcaatt gttcaactat ggttttacac agattggaga aatccttcat
     8221 atcgattggt tgatggaaaa cttattggta caagaaaata ctgtaattcc agccgtactt
     8281 tttgtggctc tctggcaagg attggctatg ccagtcatca tcttcctgtc tggtttacaa
     8341 agtattccag aagatgtgaa agaagcagca gcgattgatg gtgctactcg ttctcaacaa
     8401 ttcttcaata ttgaacttcc attcttatta ccatctatca gtatggtttt catcttggca
     8461 atgaaatcag gtttgacagc atttgacctt atctttgcat tgacaagtgg tggcccagac
     8521 ggcaaaacag agtccttggg tttactcgtt tacaactatg cctttgtaga caataaattc
     8581 tcttatgcca atgctttagc tgtagtactc ttcatcttca ttattgtcat ctcattgatt
     8641 cagatgagaa tttcgaagaa atttgaaatt taggaggaca gacatgaata ctcaaaaaca
     8701 gaaaaattgg tgggtctatc ttgtgctctt cagcggtatt ctcttcatgt ttattccgct
     8761 acttgtaacg attattagct cattcaaacc aaccaaggaa atcacgagca acttctttgg
     8821 tcttcctgaa aatttcacct taaataacta cgaacgcttg tttaatgatg ggattgtaca
     8881 atattttggc aattcagcct tgattacgat tgtagcagtt ggcttgattc tacttgtaat
     8941 tccaatggca gctttcgccg tagcccgtca aatgaaacgc cagacagtgt ttaactttat
     9001 ctactttttc ttgattattg ggatttttgt acctttccaa gtgattatgc tcccaatgac
     9061 caaattaatg tcaagccttg gtttgaacaa tattgtcggt ttgattattc tatatttgac
     9121 ctatgcagtt cctcaggccc tcttcctcta tgtcggctat attaagacta ttgttcctga
     9181 ggaaatggat gaggcggcag ctattgatgg ctgtgataaa tttaccatgt actggaaaat
     9241 tattttccca ctcatgaaac ctatgcacgc aaccgttttg attatcaatg ccctctgggt
     9301 ctggaatgat tttctcttac cgctcttggt tttgaatcgt gatcaaagca tgtggacgtt
     9361 acctcttttc caatacaatt accaaggcat gtattttagt gactatggac catcatttgc
     9421 ttcctacgtt gtaggaatta tcccaatctt attggtatac cttgtcttcc agaaacatat
     9481 catttcaggt atgacaagtg gttctgtcaa ataattacaa aggttgactt gttcaacctt
     9541 ttctaaaaat aaaaaagaaa aggcttacaa aggagaatat cgatgaaaat taaaaatcaa
     9601 gctatgttga ttacttactc agacagtttg ggtaagaatc tcaaggattt gaaaaaagtt
     9661 cttgagggac cattaaagga tgttgtggga ggtattcaca ttctaccatt cttcccatca
     9721 tcaggtgacc gtgggtttgc accaatggat tatacaaagg tagatcctgc atttggagat
     9781 tggtcagata ttgaagcatt gagtaaggac tactatctga tgttcgattt tatgattaat
     9841 catatttcag ccaagtcacc gtatttcctt gattttcttg aaaagaaaga tgaatcagct
     9901 tatgcggatt tgtttattcg ttataaaaac ttttggccaa atggtgagcc aacgcaagaa
     9961 gatgttgatt tgatctataa gcgtaaaccc cgtgctccat atcgaattgc aacatttgct
    10021 gatggtagtg aagagaaggt gtggtgtacc tttgatgagc aacagattga cttagatgtg
    10081 acaacggaga caacccgtcg tttcatccaa gaaaatctgg aacaattggc agaacatggt
    10141 gcttcgatca ttcgtttgga tgcctttgct tatgccaata aaaaactcgg aaccaattgt
    10201 ttctttgtgg agcctgatat ctgggaaatg ctacattttc cacgtcagtt attggcgcca
    10261 aaagatgtgg aaatcttacc agaaattcat gaacactata ccattcagca gaaaattgcc
    10321 gagcaagatt attatgttta tgattttgcc ttgccaatgc tagttctcca cgctttgtat
    10381 tctggtcatg tcaatcgcct ggttcactgg atggagattt gccctcgcaa gcaatttaca
    10441 actcttgata cacatgatgg aataggcgtt gtggacgtta aggatttgct gacggatgag
    10501 gagacagagg aaacgcgtga agctttgtat gcacaaggtg ccaatgtgaa gaaaatttat
    10561 agtacagaag cctataataa tttggatatt tatcagatta actgtactta ttattctgcc
    10621 cttggcaata acgatcaagc ttacctatta gcgcgtgttc tccaatgttt tgcaccaggt
    10681 attccacaga tttactatgt tggtttactt gcaggtgaaa atgatattga acttttagaa
    10741 tcaagtaaag aaggacgcaa tatcaatcgc cattactatg atttggaaga aattgagcag
    10801 gaagtgcaac gtcctgtggt acaatccttg ttcaaactcc ttaaattccg caatacaagt
    10861 ccagctttcg acggagagtt ctctgttaag atgctggatg aaacaagtat ggaaattttc
    10921 tggaataatc aagatgcagg ggtgtctgcc cgtctaacag caaacctaaa agaaaaatcc
    10981 tttgaaattg ttgaaataga agagggcaaa attaccacaa ttgagttata atatgattat
    11041 atagaaatag ggggattcct ctttttcttg cgtaaataag attgtacaga aaggatcaat
    11101 caaatgtcag aaatacaaaa gacagattgg tggaagaagt ccgtcattta ccaaatttat
    11161 cctcgtagtt ttcaagattc aaatggagat ggaattggag atattcgagg aattatcagc
    11221 cgattagatt atctccatga gcttggaatt gatgctattt ggctcagtcc tgtttatcag
    11281 tcccctatgg atgacaatgg ttacgacatc agtgactacc aaggaattgc tccagaattt
    11341 gggaccatgg aagatatgga agagctgatt gcagaaggcc ataagcgcaa tatcaaaata
    11401 atcatggatt tagtgctcaa tcatacctcg gatgaacatt tctggtttca agaagctctc
    11461 aaaggtccag ataatcccta ttatgattat tatgtttggg ctgatgagcc gaatgaatta
    11521 cgctcgacct tctcaggttc tgcttgggaa tacgttccac atctgaacaa atactatctc
    11581 caccaattct ctgttaagca accggatttg aactggaaaa atcctgctct gaaacaagaa
    11641 atctgggata tgatcaattt ttggattgaa aaaggtgttg gcggtttcag gctagatgta
    11701 attgatttga ttggaaaaga accggaaaaa ttaattacag ccgatggacc gactcttcat
    11761 cctcttattc aggaattaaa tgagaaaacc tttggtgctt acgacttagt gactgttggg
    11821 gaaacctgga gtgccaatcc agaaaatgct cagttatatt cacatcctga ccgaaaagaa
    11881 ttttcgatga ttttccaatt tgaacatgtc ggacttgatc agcaggaagg gaaagaaaag
    11941 tgggatttgg caccgcttga tccagcacgt ttgcataagg ttctatccaa atggcagact
    12001 gaactggttg gtaaaggctg gaactcgctt ttctggaata accacgattt acctcgtgcc
    12061 atttctcgct tcggtgatga tcgacctgca tttcgtgaac taagtggtaa aatgttagct
    12121 atttatcttc attttatgtc aggaacacct tatatctacc aaggtgagga aatcggtatg
    12181 atcaatactc cgattacgga tattagccaa gcggacgata ttgaaacacg tcgcatgtat
    12241 gcggaacgga ttgaaaatgg cttcaccaag gaggaattga ttacttctat aaatgccaaa
    12301 ggtcgtgata atgctcgccg tccaatgcaa tggacagcag aagaaggtgc aggtttcagt
    12361 acaggtcaag cctggctggc tttgggagat actgtcaaag acatcaacgt tgaaaaagca
    12421 cttgcagaca aacaatcgct tttctatacc tatcaaaaat taattgctct tcggaaagag
    12481 tatccttgta tgtctgaagg aaaatatgaa gtgatggaga cagggaatga cttggtaatg
    12541 gcctaccgta aatatgatga acaggatgat ttcattattt tagtcaattt cacaagcgaa
    12601 gagcaagcgt acgagttttc acttgaggac tattccgtgt ttatgcataa ttacgataat
    12661 aaagtaattt ttgataagtc tgtattgaga ccttacgaag ctatcgtttt caaaaaataa
    12721 gtggtacttc atttcttttc ccatgctttt ttcatgaatt tttggtaaaa tagaaaaaac
    12781 aaaaaagaga ggttttatca tcatgggtaa attccaagtc atttcacacc cactcattca
    12841 gcataagttg tctatccttc gtcgtacatc tacttcaaca aaggattttc gtgagttggt
    12901 taatgaaatc gcgatgctga tgggttacga agttttgcgt gacttgccac ttgaagatgt
    12961 ggagattgaa acaccaatca ctaaaacagt tcaaaaacaa attgcaggta aaaaattagc
    13021 tatagtacca attcttcgtg caggtgttgg tatggttgat ggtttgctaa gcttggtccc
    13081 agcagctaaa gttggtcaca tcggtatgta ccgcgatgaa gaaacccttc aaccagttga
    13141 gtatcttgtg aaattaccag aagatattga tcaacgtcat atttttgtag ttgacccaat
    13201 gcttgctaca ggtggttcag ccatccttgc agtggattca ttgaaaaaac gtggtgcttc
    13261 aaatatcaaa tttgttgctc ttgtttcagc tccagaaggt gtaaaagctc ttcaagaagc
    13321 acatccagat attgatatct atacggcagc cttggatgaa aaactcaatg aaaaaggcta
    13381 catcgtacca ggtttgggag atgcaggtga ccgcttgttc ggtacaaaat aattaagaaa
    13441 tcagctctgc tggtttcttt ttcatttcct gaaatcgtgt tttttatttg accaattttg
    13501 actattcata tataatgggt acagaaaacc tcgaaaagga gtaattttat gattccagta
    13561 gttattgaac aaactagccg tggtgagcgt tcttatgata tttactcacg ccttttgaaa
    13621 gaccgcatta tcatgttgac aggaccagtt gaggacaaca tggcaaactc tatcattgca
    13681 caattgcttt tccttgatgc ccaagaccct acaaaggata tttacctcta tgttaatacg
    13741 ccaggaggat cggtgtcagc aggtcttgcc attgtagaca cgatgaattt cattaaagct
    13801 gatgttcaaa ccatcgttat gggaacagct gcgagcatgg gaaccatcat tgcatcaagc
    13861 ggtgccaagg gcaaacgttt catgttgcca aatgcagagt acatgattca ccagccaatg
    13921 ggtggaactg gtggtggtac tcagcaaaca gatatggcta ttgctgcaga acacctatta
    13981 aaaacacgta ataagctaga aaaaatcttg gcagacaact caggtaagac agtcaagcaa
    14041 atccacaagg atgcagaacg tgattactgg atgtcagctg aagaaacctt ggcttatgga
    14101 tttattgacc agattatgga caatacaaaa gtcaaataag cagtgacaaa tatgtaatag
    14161 tactgactat tcctattagc tccaactctt ttacaggtag ttggagcttt tcatttatca
    14221 ttgaaaacgg tataatatag aaagagaatt tttagaggag aagacatgtt tgagaagaaa
    14281 aatcgcactt gcttaactgt ctatttacac tacaatcgtg atgcacgtaa actcagccaa
    14341 tatggagata ttgtttacca ttccaaacgc ttgcgatatg ttttggtgta tatggatcaa
    14401 gaactagtag aagctaccat attaaaattg aaaaaggaac gttttgtaaa aaaagtggtt
    14461 ccttcataca ttaaggaatt agaccaaaac tttgtaggta atctatggag agatgaagaa
    14521 ccatcagtcg taggatagtc ggaattagca ggtatgtatt ccctgctaat tttttatttt
    14581 aaaatttttt tcttttttgt tgacaatttt cagataattc agtatattag atacatcgta
    14641 aaatttaaga aagatttaag gagttttcat gaaaacaaga aaatttgcag tagcgcttgc
    14701 tacatttgcc tctgcagctc tactcgctgc atgtggtaat gtttcgacaa ccaatacttc
    14761 agccgcaggt tcttctgtag gtgatacgtt taaaattggc tacaacttgg agttgtcagg
    14821 tgctgtttca tcttatggtc aaacagagga aaaaggtgcc aaccttgccg tgaaagaaat
    14881 caatgctgcg ggtggtattg acggcaaaaa aatcgaagtg atcacaaaag acaacaagtc
    14941 tgaaacagca gaggcagcta ctgttgcaac aagcttggca agtgaagggg caaacgttgt
    15001 gattggtcct gctacatcag gagcttcagc agcctctatt ccagcattga catctgctgg
    15061 tgttcctatg attactcctt caggaactca aacaaacttg gttgtgaacg ataaaggtga
    15121 ggtccaagaa tacttcttcc gtacaacctt tacagatggg tatcaaggtc aaattatggc
    15181 taaatacgct actgagaacc tgtcagctaa gaaagttgtt ctttactttg acaactcttc
    15241 tgattatggt aagggtgtag cagaggcgtt caagaaagca tacacaggag aaatcgtttc
    15301 agaaatcaca tttgcttctg gcgataaaga tttccaagct gctttgacaa aattgaaaga
    15361 taaggaattt gatgcaatca tcatgcctgg ttactacaat gaaacaggta caattgtgaa
    15421 gcaagcacgc ggtttgggta ttgacaagcc aattcttggt tcagatggtt ttgactcacc
    15481 acaatttact gaattggcaa ctgcatcagc agcatcaaat gtttactacc tttctggatt
    15541 tgtaacatca gcaagcgaaa aagcaaaagc attctatgat gcctatgtga aagaatacaa
    15601 tgaagagcca tcaatgttct cagctttagc ttacgactcg gtttacatgg cagcagaagc
    15661 agcaaaaggt gcagctgatt cagcagcagt gaaggctaat cttgcagctc tgaaagactt
    15721 taaaggtgtg actggtacaa tgtctgttga caaagagcac aatgtcatta aatcagttta
    15781 tgttgttagt ttaacaaacg gagaagaatc atctgtagat actatctcta ttgactaata
    15841 gtggatgaaa agagggaatt ccctcttttt cacttgtcag aatgtataga atagtataca
    15901 tgcggatata aaatttgggt tggttaaatt ttctgtaatt ttaatgatta aactct
//
LOCUS       DASFSW010000035        15825 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.035, whole genome shotgun sequence.
ACCESSION   DASFSW010000035 DASFSW010000000
VERSION     DASFSW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.035"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..788
                     /locus_tag="U1099_000843"
     CDS             <1..788
                     /locus_tag="U1099_000843"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM4867177.1"
                     /translation="TSSPVNDNLMEILIMVDALKRASAESVNVVMPYYGYARQDRKAR
                     AREPITSKLVANMLQTAGVNRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERRGM
                     VGDGYVVVSPDHGGVTRARKLAQFLKTPIAIIDKRRSVDKMNTSEVMNIIGDIKDKTC
                     ILIDDMIDTAGTICHAADALADAGATAVYASCTHPVLSGPAMDNISKSAIKKLVVLDT
                     IEIAEDRLIDKIEHISTAELLAEAIIRIHEKRPLSPLFEAKSC"
     gene            879..2057
                     /locus_tag="U1099_000844"
     CDS             879..2057
                     /locus_tag="U1099_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM4867178.1"
                     /translation="MDLLNRFNKNLNRIEVSMIRQFDQSISDVPGILKLTLGEPDFTT
                     PDHVKEAAKAAIDANQSYYTGMAGLLELRQAAAEFVAEKYNLHYNPENEILSTIGATE
                     ALSASLVAILEAGDTVLLPAPAYPGYEPIVNMVGADIVEIDTTANDFVLTPEMLEEAI
                     IEQGDKLKAVILNYPANPTGVTYSREQIQAFADVLRNYPVFVLSDEVYAELTYTGQPH
                     TSIAEFLPEQTILIQGLSKSHAMTGWRIGLIMSQAPIIAQIIKSHQYLVTAASTAMQY
                     GAVEALKNGKDDALPMRAEYVKRRDYIIEKMTDLGFKIIKPDGAFYIFAKIPAGYNQD
                     SFSFLQDFARKKAVAFIPGAAFGQYGEGYVRISYAASMEKIQTAMACLKEYLEENGTN
                     "
     gene            2044..2826
                     /gene="recO"
                     /locus_tag="U1099_000845"
     CDS             2044..2826
                     /gene="recO"
                     /locus_tag="U1099_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEM4867179.1"
                     /translation="MERIETRGLVLYNRNFREDDKLVKIFTEKAGKRMFFVKHASKSK
                     LVASIQPLTYADFIVKINDDGLSYIEDFHQVQPFKNINGDIFKLSYATYILALADAAL
                     QDKVYDPALFAFLVKTLDLMESGLDYEVLTNIFEIQLLGRFGISLNFHECAFCHRVGL
                     PFDYSYKYSGVLCPQHYQQDERRAYLDPNVPYLLDQFQAISFDELETISIKPEMKRKL
                     RFFIDQLYEEYVGIHLKSKKFIDDLSSWGQIMKPRTENEETE"
     gene            2823..3830
                     /gene="plsX"
                     /locus_tag="U1099_000846"
     CDS             2823..3830
                     /gene="plsX"
                     /locus_tag="U1099_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEM4867180.1"
                     /translation="MKRIAVDAMGGDHAPQAMVEGVNQALAAFPDIEIQLYGDEAKIK
                     QYLTATERVSIVHTTEKINSDDEPVKAIRRKKDASMVLATKAVKDGQADAVLSAGNTG
                     ALLAAGVFVVGRIKNIDRPGLMSTLPTMDGKGFDMMDLGANAENTAHHLYQYGILGSF
                     YAEHVRGVKQPRVGLLNNGTEDTKGTPVHQETYKLLAEDKSINFIGNVEARELLNSVA
                     DVVVTDGFTGNAVLKTIEGTAKSIVGQLTGSIKNGGLRAKLGGLLVKPTLKKALGAMD
                     YKTAGGAVLLGLKAPVIKAHGSSDAQSIFYTIKQTRSILEAGIVEKSVAKFSVVEESH
                     D"
     gene            3823..4071
                     /locus_tag="U1099_000847"
     CDS             3823..4071
                     /locus_tag="U1099_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM4867181.1"
                     /translation="MTREQVYQRVVELIQDEKGEDFQVQPESTLADNIAADSVEIMEF
                     VLNLEDEFHVDVPDAAIEHFEVLSDIVDFIYEEAKKRS"
     gene            4189..4896
                     /locus_tag="U1099_000848"
     CDS             4189..4896
                     /locus_tag="U1099_000848"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEM4867182.1"
                     /translation="MKTDLLYSGKAKDIYATADSDQIVAVYKDQATAFNGGKKEQIVG
                     KGRLNNLISSLIFEKLNEAGVPTHFIKRLSDSEQLNKKVEIIPLEVVLRNVTAGSFSK
                     RFGVEEGITLPTPIVEFYYKKDELDDPFINDEHIAFLELASQDQIAYIKEETRRINEF
                     LKDLFAQIGLTLVDFKLEFGVDSSGQILLADEFSPDNCRLWDADGNHLDKDVFRRGLG
                     ELTEVYEVVLAKLQEVK"
     gene            4909..8628
                     /locus_tag="U1099_000849"
     CDS             4909..8628
                     /locus_tag="U1099_000849"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEM4867183.1"
                     /translation="MAKRIFVEKKADFQIKAEALRKELTHNLQLTSLSSLRLVQVYDV
                     FNLEEDLLEQAIKHIFTEQVTDKVLSDEELGLEGAVYFAIEALPGQFDQRAASSQEAL
                     LLLGSRQEVRVNTGQLYILNGDVQEEELAAIKNYLLNPVDSRFKDMDAPLVTQEFSVS
                     DTTIPSLDFFDSYGVEEFASYKREAGLAMEVEDLLFIQDYFKSIGRVPTETELKVLDT
                     YWSDHCRHTTFETELRSIDFSASKFHKQLQATYDKYLAMRTELGRTDKPQTLMDMATI
                     FGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPFGG
                     AATCIGGAIRDPLSGRSYVYQAMRISGAGDITQPLTATRSGKLPQQIISKTAAHGYSS
                     YGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPVAGDVVILLGGKTG
                     RDGIGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGDVTRLIKKSNDFGA
                     GGVCVAIGELADGLEIDLDKVPLKYAGLNGTEIAISESQERMSVVVRPQDVDTFIAAC
                     RQENIHAVVVAKVTEKPNLVMTWNGQTIVDLERSFLDTNGVRVVVDAKVVDSSVKLPE
                     TRRTSAETLQEDLKDLLSDLNHASQKGLQTIFDSSVGRSTVNHPLGGRHQLTPTESSV
                     QKLPVQQGVTTTASVMAQGYHPYLADWSPYHGAAYAVIEATARLVATGANWSKARFSY
                     QEYFQRMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLVAF
                     GVTTADSCKVLSPEFKATGEHIYYLPGQALSEDIDFALIKSNFKAFEKWQSDYVITAA
                     SAVKYGGVLESLALMSFGNQVGARVELADLETSLTGQLGGFVFTSQEDIPDAVKIGQT
                     TTDFTLVVNGVNLSGRDLQVAFEGKLEEVYPTEFEQATELQDVPAVTSSAVIKAKETV
                     EVPVVYIPVFPGTNSEYDSAKAFEQAGAKVNLVPFVTLDAESIEQSVDTMVDNIAKAN
                     ILFFAGGFSAADEPDGSAKFIVTILRNAKVRSAIDQFIEKRGLIIGICNGFQALVKSG
                     LLPYGNFEKAGETSPTLFYNDANQHIAKMVETRIANVNSPWLAGVQVGDIHAIPVSHG
                     EGKFVVTDEEFATLRDNGQIFSQYVDFTGQPSMDSKYNPNGSSHAIEGITSRNGQIIG
                     KMGHSERYEDGLFQNVPGKKDQGLFVSAVRYFTGK"
     gene            8631..10085
                     /locus_tag="U1099_000850"
     CDS             8631..10085
                     /locus_tag="U1099_000850"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEM4867184.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     LANDGGQLRRHRGTGLIAEVFKNPADLEALTGTAAIGHVRYATSGSASINNIQPFLFD
                     FADMQVGLAHNGNLTNAVSLKAELEKNGSIFSSSSDTEILMHLIRRSHNPDFMGKIKE
                     ALNTVKGGFAYLILLEDKLVAALDPNGFRPLSIGKMKNGAWVVASETCAFEVVGADWV
                     RDVEPGEIVVIDDSGIQYDSYTRDTQLAVCSMEYVYFARPDSVIHGVNVHTARKNMGR
                     RLAQEFQHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSSVVKGKRVVMIDDSIVRGTTSRRIVQLLRDAGAKEVHVAIGSPELKY
                     PCFYGIDIQTRRELISANHTVDEVCEIIGADSLTYLSLEGMIEAIGIETDAPKGGLCV
                     AYFDGEFPTPLYDYEEEYLRSLEEKTSFYIENVK"
     gene            10141..11163
                     /locus_tag="U1099_000851"
     CDS             10141..11163
                     /locus_tag="U1099_000851"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEM4867185.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERLGVMGALGGFG
                     GMFDLTKLDVKEPVLVSGTDGVGTKLMLAIQYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNEPAKLEQVVAGVAEGCVQAGCGLIGGETAEMPGMYGEDDYDLAGFAV
                     GIAEKYQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADVSGDALLPELNGRALK
                     DVLLEPTRIYVQQVLPLVKAGLVNGIAHITGGGFIENVPRMFADNLAAEIEEDKIPVL
                     PIFTALEKYGKIKHEEMFEIFNMGIGLVLAVSPDKVDSVCQLVDEEVYTIGRIIAKED
                     KSVVIK"
     gene            11160..11711
                     /gene="purN"
                     /locus_tag="U1099_000852"
     CDS             11160..11711
                     /gene="purN"
                     /locus_tag="U1099_000852"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEM4867186.1"
                     /translation="MKRIAVFASGNGSNFQVIAEQFKVAFVFSDRRNAYVLERAEKLG
                     VPTFAFELKEFSDKQTYEEAIIQLLDQHQIDLVVLAGYMKIVGPTLLAQYEGRIINIH
                     PAYLPEFPGAHGIEDAWNAGVAESGVTVHWVDSGVDTGQIIKQVRVPRLADDTLETFE
                     ARIHEAEYQLYPAVLEELGAVKK"
     gene            11721..13268
                     /gene="purH"
                     /locus_tag="U1099_000853"
     CDS             11721..13268
                     /gene="purH"
                     /locus_tag="U1099_000853"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEM4867187.1"
                     /translation="MTKRALISVSDKNGIVEFAQELTKFGWEIISTGGTKVALDQAGV
                     TTIAIDDVTGFPEMMDGRVKTLHPKIHGGLLARRDLDSHLQAANDHEIGLIDLVVVNL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYPTVLGEIAEQ
                     GETSYATRQRLAAKVFRHTAAYDALIADYFTKQVGEDKPEKLTITYDLNQPMRYGENP
                     QQNADFYQNALPTAYSIAAAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQAETIEQAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPGY
                     SAEALAILTNKKKNLRILELAFDAQDASEVEKEFTGVVGGLLVQDQDVVVESPVDWQV
                     VTERQPSEQEWAAMEFAWKSSKYVKSNGIIITNDKMTLGVGPGQTNRVASVRIAIEQA
                     KDRLEGAVLASDAFFPFADNVEEIAAAGIKAIIQPGGSVRDQDSIDMANKYGLTMVFT
                     GVRHFRH"
     gene            13333..14163
                     /locus_tag="U1099_000854"
     CDS             13333..14163
                     /locus_tag="U1099_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEM4867188.1"
                     /translation="MTLKPFTKWTGGKRKLLTQLHEHLPFEYNRYFEPFVGGGALFFD
                     LAPENAVINDFNEELINTYLQIRDNPEALLDLLHIHQENNSKEYYLDVRSVDRDGRIE
                     TMSDVERAARILYMLRVDFNGLYRVNSKNQFNVPYGRYKNPKIVDSELILSVSRYLND
                     NNILIMQGDFVTAVEEADAGDFVYFDPPYVPITATSSFTSYTHEGFSDQDQRRLRDTF
                     IDLDRRGVNVMLSNSSSPVVEELYAGFNISRVDVSRTNGASNSSRGKIQEIIVRNYGE
                     "
     gene            14153..14968
                     /locus_tag="U1099_000855"
     CDS             14153..14968
                     /locus_tag="U1099_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEM4867189.1"
                     /translation="MANNEYKYGGVLMNKPYYHKNKAILVHADTFEFLNKMKPESMDM
                     IFADPPYFLSNGGISNSGGQVVSVDKGDWDKVDSLEEKHEFNRKWIRLAKNVLKPNGT
                     IWISGSFHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKDD
                     KKARHYYNYELMKELNDGKQMKDVWVGGLTKKSEKWAGKHPTQKPEYLLERIILASTQ
                     EGDYILDPFVGSGTTGVVAKRLGRKFIGIDAERDYLKIARTRLDKEDDKKNFS"
     gene            14946..>15825
                     /locus_tag="U1099_000856"
     CDS             14946..>15825
                     /locus_tag="U1099_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEM4867190.1"
                     /translation="MTKRTFHSWFETFTDTVADWNYYVDFTKVFNNMNDLYLRTNLNI
                     LNTLVGSKQIREDFIAICDKYPDVLTVIPILLAIRLESKGRGKNRKPIALPIREYGDD
                     AIISYDFDFYSPNYAIEDYADLLENTGIFELLQSHLVKNLQDYVYGVEVGLDSNGRKN
                     RMGKIMEALVERVLQNAGLEEKTDYYKQIRTGEIESLFHIDLSSITNNGKNHKKFDFA
                     IHVNGQLYLTEVNFYSKSGSKPNETTRSYENLAEKLTEISNVHFVWITDGEGWQRSKS
                     NLEEAFERIPRLYNLHD"
BASE COUNT         4440 a         3040 c         3955 g         4390 t
ORIGIN      
        1 ccactagctc accagttaat gataatttaa tggaaatttt gattatggtc gatgctctta
       61 aacgtgcatc tgctgaatca gtaaacgtag taatgcctta ttatggttat gcacgccaag
      121 accggaaggc tcgtgcacgg gaaccaatta catcaaaatt ggtggctaat atgttgcaga
      181 cagcgggggt caatcgtttg ttgacaattg atttgcatgc tgcgcagatt cagggattct
      241 tcgatattcc tgttgatcat ttgatgggtg ctccattgat tgcggattat tttgagcgtc
      301 gtggtatggt aggtgatggc tatgttgttg tatcccctga ccatggtggt gtgacacgtg
      361 cccgtaagtt agcacagttc ttgaagacac cgattgctat tattgataaa cgccgtagtg
      421 ttgataagat gaatacatca gaagtgatga acattattgg cgatattaag gataaaactt
      481 gtatcttgat tgatgatatg attgatactg cgggtaccat ctgtcatgca gcggatgctt
      541 tggcagatgc aggggcaaca gctgtttatg cttcatgtac gcacccagta ttgtcagggc
      601 ctgctatgga taacattagt aagtcagcaa ttaagaaatt ggttgttctt gatacaattg
      661 aaattgcaga agatcgctta atcgataaaa tcgaacatat ttcaacggca gaattattag
      721 cggaagcgat tattcgtatt catgaaaaac gtcctttgtc acctttgttt gaggcaaagt
      781 cgtgttaaat aggtatgaga agagtcaaat gactcttctt ttttacattt attttcaaaa
      841 tgtattataa tagttttata tgtttttgag gtggcagaat ggatttattg aatcgtttta
      901 ataagaattt gaatcggatt gaggtttcga tgattcgtca gtttgatcag tctatttcgg
      961 atgttcccgg gattttgaaa ctgaccttgg gagagcctga ttttacaacg cctgatcatg
     1021 taaaagaagc agctaaggct gctatcgatg ctaaccagag ttattataca ggtatggctg
     1081 gtcttttgga gttgcgccaa gctgcagctg agtttgtggc tgaaaaatat aatttacact
     1141 acaatccaga aaatgaaatc ctctctacaa tcggtgcgac agaagctctg tcagcgagtt
     1201 tagttgctat tttggaggct ggggatacag ttcttctgcc tgcccctgcc tatcctggtt
     1261 atgagccgat tgtcaatatg gtgggagcag acatcgttga aatcgatacg acggcaaatg
     1321 attttgtatt gacacctgag atgttggaag aggcgattat tgagcagggt gataagctaa
     1381 aagctgtcat tctcaactat cctgccaatc caacaggtgt gacctactca cgtgagcaga
     1441 ttcaggcttt tgcggatgtc ttgcgtaact atcctgtctt tgtcctatca gatgaggtct
     1501 atgcggagtt gacttacact ggtcagcctc atacttcgat tgcggagttt ttgccagaac
     1561 agaccatttt gattcaggga ttgtccaagt cccatgctat gacaggttgg cggattggct
     1621 tgattatgag tcaggctccc atcatcgctc aaattatcaa gagccatcag tatcttgtga
     1681 ctgcagcgtc aactgctatg cagtatggtg cggttgaggc cttgaaaaat ggtaaggatg
     1741 acgctcttcc gatgcgggcg gagtacgtca agcgtaggga ttacatcatc gagaagatga
     1801 cggacttggg ctttaaaatt atcaagcctg atggagcatt ttacatcttt gcaaaaattc
     1861 ctgcgggtta caatcaagat tcctttagct tcttgcaaga ctttgcgaga aagaaagcgg
     1921 tggcctttat tccaggcgca gccttcggtc agtatggaga aggctatgtg cgaatttcct
     1981 acgcagcaag tatggagaag attcaaacag ccatggcttg tttgaaggaa tatctagaag
     2041 aaaatggaac gaattgaaac caggggatta gtcctatata atcggaattt tcgagaagat
     2101 gacaagctgg tcaagatttt tacagagaag gctggcaagc gaatgttttt cgtgaaacat
     2161 gcctctaaat ccaagctggt agcttctatc cagcctttga cctatgcgga ttttatcgtt
     2221 aaaatcaatg atgatgggct gtcttatatc gaagattttc atcaggtaca gccctttaag
     2281 aatattaacg gtgatatttt caagcttagc tatgctacct atatcttggc cttggccgat
     2341 gcggccttgc aggacaaggt ctatgaccca gctctctttg cttttttggt caagaccttg
     2401 gacttgatgg agtcaggttt ggactacgaa gttctgacca atatctttga aattcagctc
     2461 ttgggtcgat ttgggatcag tctgaatttt cacgagtgtg ctttttgtca tcgggttggc
     2521 ttgccttttg actattccta caagtacagc ggtgtcttgt gtccgcaaca ctatcaacaa
     2581 gatgagcgac gggcttatct ggatccaaat gttccctatc tacttgatca atttcaggct
     2641 atttcctttg atgagctgga aaccatttcc atcaagcctg agatgaagcg aaaattacgg
     2701 ttttttattg accagctgta cgaggaatat gtggggattc acttgaaatc caagaaattt
     2761 atagatgatt tgtcttcttg ggggcagatt atgaaaccaa gaacagaaaa tgaggaaaca
     2821 gaatgaaacg tattgcagta gatgctatgg gtggagacca cgcccctcag gcaatggtag
     2881 aaggtgtcaa tcaagccttg gctgcctttc cagacattga aattcaactt tatggtgatg
     2941 aggctaaaat caagcagtat ttgacagcga cagagcgtgt cagcatcgtc catacgacgg
     3001 agaaaatcaa ttcagatgat gagcctgtca aggcgattcg tcgtaagaaa gatgcttcga
     3061 tggtcctagc gaccaaggct gtcaaggatg gtcaggcaga tgcggtcttg tcagctggaa
     3121 atacaggtgc tcttttggcg gcaggcgtct ttgtcgtcgg tcgcatcaag aacatcgacc
     3181 gtcctggtct tatgtctacc ttgccgacta tggacggtaa gggatttgat atgatggatt
     3241 tgggggcaaa tgctgaaaat acagctcatc acctctatca gtatggtatt cttggctcat
     3301 tttacgcgga gcacgtgcgt ggtgtcaaac aacctcgtgt gggacttttg aacaacggta
     3361 cggaagatac caagggcacg ccagttcacc aagaaaccta taaactcttg gcggaagaca
     3421 agtcaatcaa ctttatcggt aatgtggagg cgcgtgagtt gctcaacagt gtggcagatg
     3481 tggtcgtgac ggatggtttc acgggaaacg ctgtgctgaa aaccattgag ggaactgcaa
     3541 aatccatcgt tggtcagttg actggctcaa tcaagaatgg cggtctgcgt gctaaattgg
     3601 gaggcttgtt ggtcaaacca accttgaaaa aggccttggg ggctatggac tataaaactg
     3661 caggtggtgc tgtcttgctt ggtctgaaag cacctgtcat caaggcgcac ggatccagcg
     3721 atgcccagtc gattttctat acgattaagc agacgcgttc gattttggag gctggcattg
     3781 ttgaaaaatc agtggccaaa ttttcagtag tggaggagag tcatgactag agagcaggtc
     3841 tatcagcggg ttgttgaatt aattcaggat gaaaagggag aagattttca agttcaacct
     3901 gaatctactt tggcagataa tattgcagca gattcagtag aaatcatgga atttgttctt
     3961 aatttggagg atgaatttca tgtagatgtt ccagatgctg ctattgagca ttttgaggtc
     4021 ctatctgata ttgttgattt tatttatgaa gaagcaaaga aacgttcgta gtttacgggc
     4081 gtttttcttt gttttttcct aaaaaaccgt aaatttgttg tgaatgcgaa tattgtgtgt
     4141 tagaataaac gaaaagacta gaatatttta cgaaaggcga acattctcat gaaaacagac
     4201 cttctctatt caggaaaagc caaagacatc tacgctacgg ctgatagcga ccagattgtt
     4261 gcggtttata aggatcaggc aacggctttt aatggtggta agaaagaaca gattgtgggc
     4321 aagggccggc tcaataatct gatttcatct ttgatttttg aaaaattgaa tgaagctggt
     4381 gtcccaacgc attttatcaa gcgtttgtcg gattcggagc agttgaataa gaaggtggag
     4441 attattcctc ttgaggttgt tttgcgaaat gtgacagctg ggtctttctc aaaacgcttc
     4501 ggtgtggaag aagggataac cttacctact cctatcgtgg aattttacta taaaaaagat
     4561 gagttggacg atccttttat caacgatgaa cacattgcct ttctagaact ggctagtcag
     4621 gaccaaatcg cctatatcaa ggaagaaaca agacgaatca atgaattctt gaaggacttg
     4681 tttgctcaga ttgggctgac tttggtggac ttcaagctag aatttggggt tgactcgtct
     4741 ggtcagattt tattggcgga tgagttttcg cctgataatt gtcgtttgtg ggatgcggat
     4801 ggcaaccatc tggacaagga tgttttccgt cgggggctcg gggagttgac cgaggtttac
     4861 gaggtcgtat tggcaaagtt acaagaagtg aaataaggat tggaaaatat ggcaaagcgg
     4921 atttttgttg agaagaaggc ggattttcag attaaggcgg aggctcttcg caaggagttg
     4981 actcataatt tacagttgac aagcctgtca agtttgcggt tagtgcaggt ttacgatgtc
     5041 ttcaatcttg aggaggactt gttagagcag gccatcaagc atatctttac agagcaggtg
     5101 acggacaagg ttttgtcgga tgaagaatta ggtctggaag gtgcagttta ttttgctatt
     5161 gaggctttgc ctgggcagtt tgaccagcgg gctgccagca gtcaggaggc ccttctctta
     5221 ttgggcagtc gtcaggaggt gcgtgtcaat acaggtcagc tctatatttt gaacggtgat
     5281 gtgcaggaag aagaattagc tgccatcaag aattatttgc tcaaccctgt ggattcgcga
     5341 ttcaaggata tggacgctcc tttggtgact caggagtttt cagtttcgga tactactatt
     5401 ccaagcttgg acttttttga tagctacggg gtagaggaat ttgcgtctta caagcgtgag
     5461 gctggtttgg ctatggaagt ggaagacctg ctcttcattc aggattactt caagtcaatt
     5521 ggtcgagtgc caactgagac agaactcaag gtcttggata cttactggag tgaccactgc
     5581 cgccatacga catttgagac agaacttagg tctattgact tttcagcttc aaaattccac
     5641 aagcaattgc aggcgactta tgacaagtat ctggctatgc gaacagagtt aggtcggaca
     5701 gacaaaccgc agacgcttat ggatatggcg acgatttttg gtcgctatga gcgagcaaat
     5761 ggtcgtctgg atgacatgga agtgtcagat gaaatcaatg cttgctcggt ggaaatcgaa
     5821 gtagatgtgg atggcgtgaa agagccatgg ctcctcatgt tcaagaatga aacccataat
     5881 cacccaacag aaatcgagcc ttttggtggt gccgcaacct gtatcggtgg agccattcgt
     5941 gatcctttgt caggtcgttc ttatgtttat caagccatgc ggatttcagg tgcaggcgat
     6001 attacccaac ctctgacagc tactcgctca ggaaaattgc cacagcaaat catttcaaaa
     6061 acagcggcac atggttattc ttcatacgga aaccaaatcg gtttggcgac aacttatgta
     6121 cgtgaatatt tccacccagg ctttgtcgca aaacgtatgg aattaggtgc cgttgtcggt
     6181 gctgctccta aggaaaatgt ggttcgtgaa aaaccagtcg caggcgatgt ggttatcttg
     6241 ttaggaggca aaacaggtcg agatggtatc ggtggggcaa caggctcgtc caaggtacag
     6301 acagttgagt ctgtggaaac agctggtgcg gaagtccaaa aaggaaatgc cattgaagaa
     6361 cgcaaaatcc aacgcttgtt ccgaaatggt gacgttacac gcctgataaa aaaatccaat
     6421 gacttcggtg caggcggtgt ctgcgtagct attggggaat tggcggacgg tcttgaaatc
     6481 gatttggata aggttcctct aaaatatgca ggcttgaatg gaacggaaat cgctatttct
     6541 gaatcacagg agcggatgag tgtcgttgtt cgtcctcaag atgtcgatac cttcatcgca
     6601 gcctgccgtc aggaaaatat ccatgcggtt gttgtagcca aagtgacgga aaaaccaaat
     6661 ctggtcatga cttggaatgg tcaaaccatt gtcgacttgg aacgttcctt ccttgatacc
     6721 aacggtgtgc gcgtggtggt ggatgccaaa gtggttgaca gttctgtcaa gctaccagaa
     6781 acacgtagaa cttccgctga aacgctacag gaggacttga aagaccttct atcagacctc
     6841 aaccatgcta gccagaaagg tttgcagacg attttcgact catctgttgg tcgttcaacc
     6901 gtcaaccacc cactcggagg tcgtcaccag ctgactccga cagaaagttc ggtgcaaaag
     6961 ttgcctgtcc aacagggtgt gacgacgaca gcatcagtca tggctcaggg ttaccatcct
     7021 tacctagcag actggtcacc ttatcacgga gcggcttatg cagtcatcga agcgacagct
     7081 cgcttggtgg caacaggtgc taactggtcc aaggctcgct tctcctatca ggagtatttc
     7141 cagcggatgg acaagcaggc tgagcgtttt ggtcagccag tagcggcttt gcttggttct
     7201 atcgaggctc agattcagct tggcttgccg tctattggtg gcaaggactc catgtctggt
     7261 acctttgagg acttgacagt tccgccgacc ttggttgcct ttggcgtgac cacggcagac
     7321 agctgcaagg ttctatcacc tgagttcaag gcgactggcg agcatatcta ttacctacca
     7381 ggccaagcct tatcagaaga tattgatttt gccttgatca agtctaattt taaggctttt
     7441 gaaaaatggc agagcgatta tgtgattacg gctgctagtg cagtcaagta tggaggagtt
     7501 ctagaaagtc tagcccttat gtcctttggt aaccaagtcg gagcaagagt tgagcttgcc
     7561 gaccttgaaa ctagcttgac aggtcaactt ggtggctttg tcttcacatc gcaagaagac
     7621 attccagatg ctgtgaaaat cggtcaaacc actacagact ttacactggt tgtcaatggt
     7681 gtcaaccttt ctggacggga cttgcaagta gcttttgagg gcaaactaga agaagtttac
     7741 ccaacagagt ttgaacaggc gacggagttg caggatgttc cagcagtcac aagttcagcg
     7801 gttataaaag caaaagaaac cgttgaagtg cctgtagttt acattccagt tttcccaggt
     7861 accaactcag aatatgattc tgccaaggcc tttgaacagg ctggtgcaaa agtcaatctt
     7921 gttccatttg tgaccttgga tgcagagagc attgaacagt ctgtggacac tatggttgac
     7981 aatattgcta aggcgaatat tctattcttt gcaggtggat tctcggctgc ggatgaacca
     8041 gatgggtctg ctaagtttat cgtgactatc ttacgaaacg ccaaggtccg ctctgctatt
     8101 gaccaattta tcgaaaaaag aggcctcatc atcggtatct gtaatggttt ccaggccctt
     8161 gtcaaatcgg gcttgttgcc gtatggaaac tttgagaagg cgggtgaaac cagtccaacc
     8221 ctcttctaca acgatgccaa ccaacacatt gctaaaatgg tggaaacgcg gattgccaat
     8281 gtcaactcac cgtggttggc aggtgtccaa gtcggcgaca ttcacgctat cccagtttcc
     8341 cacggtgaag gaaaatttgt agtgacggac gaggaatttg ctactttgcg tgataatggg
     8401 caaatcttca gccaatacgt tgactttacc ggtcagccaa gcatggattc taagtacaat
     8461 ccaaatggat ccagccatgc cattgagggc attaccagtc gcaacggtca gattataggg
     8521 aaaatggggc attccgagcg ttacgaggac ggtcttttcc aaaatgttcc aggtaagaaa
     8581 gatcaagggc tctttgtttc agcagttcgc tattttacag gaaaataaat atgacatacg
     8641 aagttaaatc actcaacgaa gaatgcggtg ttttcggcat ttggggacat ccgcaggctg
     8701 ctcaggtgac ctattttggt ctccatagtt tgcagcaccg tggacaagaa ggagcaggga
     8761 ttttagccaa tgatggcgga caattgcgtc gccatcgcgg aacaggcctg attgcagaag
     8821 tcttcaaaaa tccagcagat ttagaagcct tgacaggaac ggctgctatt ggtcatgtcc
     8881 gctatgcaac ttctggctct gcttctatca ataatatcca gcccttccta tttgattttg
     8941 cagatatgca ggtgggtttg gcacacaatg ggaatttgac caacgcggtc agtttgaagg
     9001 ctgaacttga aaaaaatggt tccattttct cttcttcttc tgacactgag attctcatgc
     9061 acttgattcg ccgcagtcac aatccagact ttatgggaaa aatcaaggaa gccctcaata
     9121 ctgttaaagg gggctttgcc tacctgatct tgttggaaga caaattggtt gctgccttgg
     9181 accctaacgg tttccgtcca ctttcaatag gcaaaatgaa aaatggtgcc tgggtagttg
     9241 ccagcgaaac ttgtgctttt gaagtagtgg gggctgactg ggtgcgagat gtggagcctg
     9301 gtgaaattgt cgtcattgat gattcaggaa ttcaatatga tagctataca agagatacgc
     9361 aactggctgt ttgctctatg gagtatgtct attttgctcg cccagattcg gtaattcacg
     9421 gggtcaatgt ccacacggct cgtaaaaata tgggtcgtcg tttggcccag gaatttcaac
     9481 atgaagcaga tatcgtggtt ggtgtgccaa actcctccct atctgcggct atgggatttg
     9541 cagaggaatc tggtttgcca aatgaaatgg ggctgattaa aaaccaatac acccagcgga
     9601 cctttattca accaacccag gaattgcggg agcagggtgt gcgcatgaag ttgtcagccg
     9661 tttcaagtgt tgtcaaaggc aagcgtgtgg tcatgattga cgactctatt gttcgcggta
     9721 caactagccg ccgcattgtc cagcttcttc gtgatgcagg ggcaaaagaa gtccatgtgg
     9781 ctatcggcag cccagaactc aagtacccgt gtttctatgg tattgatatt cagacccgta
     9841 gggagctgat ttcagccaac cataccgttg atgaagtctg tgagattatc ggggcagaca
     9901 gtctgaccta cctttctctt gaggggatga ttgaagccat cggcatcgaa accgatgcgc
     9961 caaaaggcgg gctctgtgta gcctactttg acggagaatt tccaacgcct ctctatgact
    10021 atgaggaaga ataccttcgt agcctagaag agaaaacgag tttctacatt gaaaatgtca
    10081 agtaaagaca gaggtgtcaa cgttacttga cagacctgta aactgttgaa aggaatcaaa
    10141 atgacaaata aaaatgccta cgcccaatcg ggcgttgacg tcgaagcggg atatgaagtt
    10201 gtcgaacgca tcaagaagca tgtagctcgg acagaacgct tgggtgtgat gggagccctc
    10261 ggtggttttg gcgggatgtt tgatctaacc aaactggatg tcaaagagcc agtcttggta
    10321 tctgggacag acggtgtggg caccaagctc atgctggcta tccagtacga caagcatgac
    10381 accattggtc aagattgcgt ggctatgtgt gtcaacgaca ttatcgcagc aggtgcagag
    10441 ccgctttact tccttgacta cattgcgact ggaaaaaatg agccagccaa gctggagcaa
    10501 gtagtagccg gtgttgcaga aggctgtgtc caagctggtt gcggattgat tggcggtgaa
    10561 acggctgaaa tgcctggtat gtacggcgag gatgactatg acctggctgg ctttgctgtc
    10621 ggtattgcgg agaagtatca gattatcgac ggtagcaagg tcaaggaggg tgacattctc
    10681 ctcggcctag cttccagcgg tatccactcc aacggatatt ccctagtccg tcgtgttttt
    10741 gcagatgttt ctggggacgc tctgttgcca gagctcaatg gcagagctct taaggatgtc
    10801 ctattagagc cgacacgtat ctatgtccag caggtgttgc ctctggtaaa agcaggtctg
    10861 gtcaacggca ttgcccatat cacaggcggt ggatttatcg aaaatgtgcc tcgtatgttt
    10921 gcggacaacc ttgcagctga aattgaagaa gataagattc cagtcctccc aatctttacg
    10981 gcccttgaaa aatacggaaa gatcaagcat gaagaaatgt ttgaaatctt caatatgggt
    11041 atcggattgg tcctggcagt cagtccagac aaggttgaca gtgtctgtca actagttgac
    11101 gaggaagttt acaccattgg tcgcatcatc gccaaggaag ataagagtgt ggtcatcaaa
    11161 tgaaacgaat agcagtgttt gcatcaggca acggctccaa tttccaagtc atcgcagaac
    11221 agtttaaagt agcttttgtc ttttcagacc gcagaaatgc ctatgtcttg gaacgagctg
    11281 aaaaactagg tgtaccaacc tttgcttttg aactaaaaga gttttcagat aagcagacct
    11341 acgaagaagc cattatccaa ctattagacc agcaccagat tgacttggtg gtcttggcag
    11401 gctatatgaa aattgtgggt ccgaccttgt tagctcaata tgaaggtcgt atcatcaata
    11461 tccacccggc ttatttgcct gaatttccag gagctcacgg gattgaagac gcttggaatg
    11521 caggtgtggc agaaagtggt gtgacagttc actgggttga cagcggtgtt gacacaggac
    11581 aaatcatcaa acaagtccga gtgccccgac tggccgatga taccctggaa acctttgaag
    11641 ccagaataca tgaagcggag taccaactct atccagcggt tttggaggag ttgggggcag
    11701 tgaaaaaata aggagaaatc atgacaaaac gtgcgttaat tagcgtatca gataaaaatg
    11761 gtattgtaga atttgcccaa gaattgacca agtttggttg ggaaattatc tcgaccggtg
    11821 gtaccaaggt tgccttggat caggctggtg taactaccat cgccattgac gatgtgactg
    11881 gttttcctga gatgatggac ggccgtgtta agacgcttca tccgaaaata cacggtggtt
    11941 tgttagcacg tcgggatttg gacagccacc tgcaagcagc taacgaccat gaaataggct
    12001 tgattgactt ggtagtggtt aacctttacc ccttcaaaga gactatttta cgtccagatg
    12061 tgacctatga tttggcggtg gagaacatcg acatcggcgg accgtccatg cttcgttctg
    12121 cagccaaaaa ccacgccagc gtgaccgttg tggtggatcc ggcagattat ccgacggttt
    12181 taggggaaat tgcagagcag ggggaaacga gctatgcaac acgtcagcga ttggctgcaa
    12241 aggtattccg tcataccgca gcttatgatg ccctaatagc agattatttt accaagcaag
    12301 taggcgaaga taagcctgaa aaattaacca ttacttatga cctcaatcag cctatgcgtt
    12361 acggtgaaaa tccccagcaa aatgcggatt tctaccaaaa cgcccttccg acagcctatt
    12421 ctattgcagc tgctaagcag ttaaatggta aggagttgtc tttcaacaac attcgtgatg
    12481 cggatgcggc cattcggatt atccgtgatt ttaaggaccg tccaactgtt gtggctctca
    12541 aacacatgaa cccttgtggt atcgggcagg cagaaaccat tgaacaggct tgggattatg
    12601 cttatgaggc tgacccagta tcgattttcg gaggcatcgt cgtgctgaac agggaagtgg
    12661 atgctgcgac ggctgaaaag atgcacccga ttttcttaga aatcatcatc gcaccgggct
    12721 actcggcaga agcgctagct attttgacca ataaaaagaa aaatcttcgg attttggagt
    12781 tggcctttga cgcacaggat gcaagcgaag tggaaaaaga gttcacaggc gttgtcggcg
    12841 gactcttggt gcaggatcag gacgtggtgg tggaaagccc agtggactgg caggtggtga
    12901 ccgagcgtca accgtccgag caagagtggg cagccatgga gttcgcttgg aagtcctcta
    12961 aatatgtcaa gtccaacggc atcatcatca ccaatgacaa gatgactttg ggcgtggggc
    13021 cggggcaaac caaccgtgta gcgtccgtcc gtatcgctat cgagcaagcc aaggaccgtt
    13081 tggagggagc cgttttggcg tcggacgcct tcttcccatt tgctgataac gtggaagaaa
    13141 ttgcagctgc aggtatcaag gctatcatcc agccaggtgg ttctgtccgt gaccaagact
    13201 ccattgacat ggccaacaag tacggcttga ccatggtctt tacaggagtc agacatttta
    13261 gacattgaga attttggata agtatagcac cccagctgga gaagttgggg tggattttgg
    13321 tataatagtt ccatgacttt aaaacctttt acgaaatgga caggtgggaa gcggaagcta
    13381 ttaactcagc tacatgaaca tctaccattt gaatacaata gatattttga accgtttgtt
    13441 ggaggagggg cattattctt tgatttagct cccgaaaatg ccgttataaa tgattttaat
    13501 gaggaattga taaatactta tttacagata cgggataatc cagaagcatt gcttgattta
    13561 ttacatatcc atcaagagaa caattccaag gaatattatt tggatgttcg ttcggttgac
    13621 agagatggta gaatagaaac tatgtccgat gtggagcgag cagcacgcat actatatatg
    13681 ctacgagtag attttaatgg tctatatagg gtgaactcta aaaatcagtt taatgtccct
    13741 tacggcaggt ataaaaatcc aaagatagta gactcggaac ttattctaag tgtatcaaga
    13801 tacttaaatg ataacaatat tttaattatg caaggtgatt ttgttacggc agttgaagaa
    13861 gctgatgcgg gagattttgt atactttgat cctccgtatg ttcctataac ggctactagc
    13921 tcttttactt cttatacgca tgaaggtttt tctgatcagg atcagcgtcg attaagagat
    13981 actttcatag atttagaccg aagaggagtt aatgtaatgc tttctaattc ttcaagccca
    14041 gtagttgaag aattgtacgc tggttttaat atttcccgag tagatgtatc gagaacgaat
    14101 ggagcaagta attccagtcg tggaaaaatt caagaaatta ttgtaagaaa ttatggcgaa
    14161 taacgaatat aagtatggag gggtcttaat gaacaaacca tactatcata aaaacaaggc
    14221 tattttggta cacgcagata catttgaatt tttgaataaa atgaaaccag agagcatgga
    14281 tatgattttt gcagatcctc catatttttt aagtaatgga ggaatttcta actctggagg
    14341 tcaagttgtt tctgtagata agggggattg ggataaagtt gattctttag aagaaaaaca
    14401 tgaatttaat aggaaatgga ttcgcttagc caaaaatgtg ttgaaaccaa atgggaccat
    14461 ttggatttca gggagctttc acaatattta ttcagtgggt atggccttag aacaagaagg
    14521 ttttaaaata ctaaataata ttacttggca gaagacaaat cctgcaccaa atctttcctg
    14581 tcgttacttt actcattcaa cggaaactat tttatgggct cgaaaagatg ataaaaaagc
    14641 tcgtcattat tataattacg aacttatgaa agaactcaat gatggcaaac aaatgaaaga
    14701 tgtttgggtt ggaggactaa caaaaaaatc tgaaaaatgg gcaggtaagc atccgactca
    14761 gaaaccagag tatctcttag aacgtattat tttagcttca actcaagaag gagattacat
    14821 acttgatcca tttgtaggaa gcgggacgac aggagtggtt gccaaacgtt tgggaagaaa
    14881 atttattggg attgatgctg aacgggatta tttaaaaatc gcgagaacta gattggataa
    14941 ggaagatgac aaaaagaact tttcatagtt ggtttgaaac ttttacagat acggttgctg
    15001 attggaacta ctatgttgac tttacaaaag tttttaataa tatgaatgac ctgtatttga
    15061 gaactaattt gaatatcttg aatacgttgg tagggagtaa acaaattcga gaagatttta
    15121 tcgcaatatg tgacaagtat ccagatgtgt taactgtcat accaattctt ttggctatac
    15181 gtttggaatc taaaggccga ggaaaaaatc gtaaaccaat tgctttacct attcgcgagt
    15241 atggggacga tgctataata agctacgatt ttgattttta ttctcctaac tatgctatag
    15301 aggattatgc cgatttacta gagaatacag gtatttttga actactgcaa tcacatctag
    15361 ttaaaaactt acaggactat gtttacggtg tagaagtcgg gttagattca aatggtcgta
    15421 aaaatagaat gggtaagata atggaagctt tggttgaaag agtacttcag aatgcaggac
    15481 tagaagaaaa aacggactac tataaacaaa tacgaactgg ggaaattgaa tcactatttc
    15541 atattgatct aagttctata acgaataatg gtaaaaatca taagaaattc gattttgcaa
    15601 ttcacgtaaa tggtcaattg tatttaacgg aagtaaattt ctattctaaa tcagggtcaa
    15661 aaccaaatga aaccacacgt tcatatgaaa atttagcaga aaaacttact gaaatttcga
    15721 atgttcattt tgtttggata acagatggtg aaggttggca aaggtctaaa tctaatttag
    15781 aggaggcatt tgaaagaata ccaagattgt acaatctcca tgata
//
LOCUS       DASFSW010000036        15589 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.036, whole genome shotgun sequence.
ACCESSION   DASFSW010000036 DASFSW010000000
VERSION     DASFSW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15589
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.036"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(2..346)
                     /locus_tag="U1099_000857"
     CDS             complement(2..346)
                     /locus_tag="U1099_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867191.1"
                     /translation="MKHIVTKRNYKVGACAMALALLMTTAGGAIVNAEQRLNSPVGFE
                     QRDEVLLELKESTQPFGQGVQHLEPINSPATQMAPSVQKSSWFSSFWGGVKTFWNALC
                     CSVGLTGLKVSK"
     gene            complement(813..1517)
                     /locus_tag="U1099_000858"
     CDS             complement(813..1517)
                     /locus_tag="U1099_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEM4867192.1"
                     /translation="MKIHVVNNQVEGATVALDILREKLNGGAKVLGLATGSSPLEFYR
                     LIRESDLDFSNVTSVNLDEYVGLGEESDQSYIHFMKENLFNDKPFKKSYLPNGLATDV
                     VAETERYNKILAEHPVDFQILGIGRNGHIGFNEPGAPFDGQTHLVELAPSTIEANARF
                     FDNPEDVPKQAISMGIANIMAAKTIVLMAYGQEKADAIKATVEGAVTEDVPASVLQNH
                     DNVILILDQAAASKLV"
     gene            1865..2893
                     /gene="queA"
                     /locus_tag="U1099_000859"
     CDS             1865..2893
                     /gene="queA"
                     /locus_tag="U1099_000859"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEM4867193.1"
                     /translation="MNTADFDFDLPEELIAQVPLEKRDSSKLLILDREKQSMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGYKPETMGHVELLLLKNTQGNQWEVLAKPAKRL
                     KVGTTVAFGDGRLTATIVEELEHGGRIVEFSYEGIFLEVLESLGEMPLPPYIHEKLED
                     RERYQTVYAKENGSAAAPTAGLHFTQELLEKIEAKGVKLVYLTLHVGLGTFRPVSVDN
                     VDEHEMHSEFYNLSAEAAQTLNQVKDSSGRIVAVGTTSIRTLETIGNKFDGRLEADSG
                     WTNIFIKPGYTFRIVDAFSTNFHLPKSTLVMLVSAFAGREFTLDAYKHAVEERYRFFS
                     FGDAMFIQ"
     gene            2942..3415
                     /locus_tag="U1099_000860"
     CDS             2942..3415
                     /locus_tag="U1099_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM4867194.1"
                     /translation="MNKIESRHQLILSLIMEKKIHTQQELQELLEINGVSVTQSTLSR
                     DIKMLNLVKVNEDDSSHYVINPIAPTRWEKRLRLYMEDALVMLKPIQHQVVLKTLPGL
                     ANSFGSILDAMEIPQIVATVCGDDVCLIICEDVEGAQACFEHLKQFTPPFFFSKL"
     gene            complement(3708..7967)
                     /locus_tag="U1099_000861"
     CDS             complement(3708..7967)
                     /locus_tag="U1099_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEM4867195.1"
                     /translation="MKKETSKFTKKVILGQLLICTSFLVWAGQTVAAEEVSQGEPVAI
                     NNETPPAIEAKPLVEATPSTETEPSTPEEKQEEPQVADKNVVEGKKPVTNSQHQMVSE
                     LSTVPSVSIKNPDAATDGSAYGSSDDANSNTKIIAGKETGESDNGYSKWDHVYVQYDF
                     EKEVPVHTVKIHRNTYDNAVSTFKDVKVELSTSVDFSPETTEVIFKQADVEETIANKG
                     QPQVIQLPAPINARYIRIWGRGHYIQNTNSSWKGYSNGVLFNEIEVLAQVEADAGTNE
                     EPQTELVNLAKLKKPYFYGKAPTNPEAINDGKFDENYTTQESIGNQAYLQYEFRNVYT
                     IEEIKVQLAPGEYRNFRIDLDDDYNDGNNYNTIYKNENFTIDKEQIITIKTPKNSKAR
                     YVRFRGTRIDDTPLRYSEIQIMGRGKSYDESAPEYIPPKSEYDTLVWSDEFNGDKIDE
                     SKWQIIDGMWNHAAIYNRKAVSIKKDGDKSYLSIRSTNHKDKKTLVEAVGHDRYDDKE
                     LPDKVTWSSGRLESKDKYSFQFGRMAVRAKVNDSKGIWPAIWMLAQDETGHDEIDVLE
                     YLGQNPWDAWTTNHFGILAKNKKSDGSAYKNYEAWSQEFHVYEVEWTPDVIKWYIDGK
                     FVFQTDRGKDRDGMHTRPMFPILETQVGDGWVGDVDYKRNMTKQDSEYLVDWVRIYQK
                     ADQSQVHFDNLDDAKPNDYVIKPQQKLGHLVTVSNGKAAHENKNNFYYGGQPRYETSR
                     LYADGDGENALVYKIANPEALHLTTYYKTLEDYSVYNKEAGANEGKSVRKHLINAKDG
                     EIDFTVYSSVDGKSWNKENVTVVDNFVEATPAYARTTFDIRNIRKGTKFVKIVFPTVK
                     GISYKLEDGTEKGLQASDVQLAKVTFVATHPEAEPEKPIVDQEKLALSKVFSLDAGRK
                     YFSVNQIKEIIDLISSYGYTDMNLLLGNDGLRFLLDDMNLTVGDKVYDSEAVRKAIIN
                     GNKHYYDDPNGTTLSQSEMDDILAYAKEKNISIIPTINSPGHMDAIVEAMEELGIKSP
                     KFSYNGKTSARTVDLKNDQAVAFTKALIDKYASYFAGKSKIFNIGLDEYANDATNASG
                     WHILQTQGDYGKFIQYANDLAAIVKKHGLEPMAFNDGIYYNHDTSSGTFDKDIIASFW
                     TGGWGGYDVASSKSLHEKGHKILNTNDAWYYVIGREAEGLGWYNLDQGLRGTKSTRFD
                     QVPKSENTKVPIIGSMVAVWADDPSKPYSYDSIKKLLHSFTENNSDYFLAEYRDLREE
                     VGKIPTDLNSYTPESVATLKEVLNKIDWKLSRNNQAFVDGYLAEVKAAREALVPVTAK
                     GDSVKAESLPEGKLPLITAKGKSVQAEPRPAFKGYLTSNYGPSVTPLSLVSKPSSEQS
                     RSQQAASQKKELPNTGAVDGLGFSLLGMLGLAFASRRRKEG"
     gene            complement(8338..9678)
                     /locus_tag="U1099_000862"
     CDS             complement(8338..9678)
                     /locus_tag="U1099_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEM4867196.1"
                     /translation="MRTSFIQSSHQEACIQAIQELVAFPSVLQEHQADTPFGQAIQDV
                     LEHTLALTEKMGFKTYLDPAGYYGYAEIGQGEELLAILCHLDVVPAGDLSQWQTPPFE
                     AVVEGDYIIGRGVQDDKGPSMAALFAVKALLDAGVQFNKRIRFIFGTDEETLWRCMNR
                     YNQLEEVATMGFAPDSSFPLTYAEKGLLQAKLHGPGHPCLSIEAGTAYNVVPAKASYS
                     GHLLAGVIAELDQLGFDYETKDDQVTVLGISRHAKDAAEGVNAIVRLAKALEHFESHP
                     ALDFIVNAVGEDATGFKLFGDVTDEPSGTLSFNIAGLTISAKKSEIRLDIRIPVTADK
                     EALVSTLQAKAQSCGLTYEEYDYLASLYVPLDSQLVSTLMSVYQDKTGDLTSEPISSG
                     GATFARTMPNCVAFGACFPDTEQTEHQENERMPLEDLYKTMDIYAEAVYRLAAE"
     gene            complement(9797..11299)
                     /locus_tag="U1099_000863"
     CDS             complement(9797..11299)
                     /locus_tag="U1099_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEM4867197.1"
                     /translation="MSEKVKKGFKLPSSYTILMLIIAFMAVMTWIIPAGQYQVDEAGR
                     LVAGTYEKVAQNPQGIYDVFMAPVRAMLGHGATEAAINVAFFIIMVGAFLGVVNETGA
                     LDVGIASIVKRFKGREKMLIYILMPLFALGGSTYGMGEETMAFFPLLVPVMMAVGFDS
                     ITGVAIILLGSQIGCLASTVNPFATVVASDAAGVSVADGMIWRLVFFVVILLMGVLFV
                     ANYAEKVKNDPTKSLVYKQREADMQHFNVTATQEVNAELSPAQKRVLWVFVLTFVLMI
                     CSFIPWEDLGVTIFTQFKDWLIGLPFIGQVIGSSTAALGTWYFPEGAMLFGIAGIVVG
                     LVYGMSEERLVKSFMFGAADIFSVALICAIARGIQVIMNDGMITATILHMGEEGLQGL
                     SSQVFIILTYLFYLPMSFLIPSSSGLAGASMGIMAPLGEFVNVPASLVITAFQAASGL
                     LNLVAPTSGIVIGALALGRVEIGTWYKFVGKLIVGIMIASIAILVIATFF"
     gene            complement(11642..12589)
                     /gene="arcC"
                     /locus_tag="U1099_000864"
     CDS             complement(11642..12589)
                     /gene="arcC"
                     /locus_tag="U1099_000864"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEM4867198.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQKEALVQTAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQNLAADSEKNPAFPLDSLVAMTEGSIGFWLQNALENELHKE
                     GIEKDVASVVTQVIVDKNDPAFENLTKPIGPFYTEEEAKAEAEKTGATFKEDAGRGWR
                     KVVASPKPVGIKEIGTIRTLLNAGEVVVAAGGGGIPVVQEADGTLTGVEAVIDKDFAS
                     QCLAELVDADLFIVLTGVDYVFVNYNKPDQEKLETVTVAELEEYIKQNQFAPGSMLPK
                     VEAAIAFVNNKPQSKAVITSLENLGALIESDSGTIIVKG"
     gene            complement(12707..13720)
                     /gene="argF"
                     /locus_tag="U1099_000865"
     CDS             complement(12707..13720)
                     /gene="argF"
                     /locus_tag="U1099_000865"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEM4867199.1"
                     /translation="MTNVFKGRHFLAEKDFTRAELEWLIDFSAHLKDLKKRNIPHRYL
                     EGKNIALLFEKTSTRTRAAFTVASIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQKMVEELAEFSGVPVWNGLTDAWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEVVALAEVFAKESGARVLITDNA
                     DEAVKGADVLYTDVWVSMGEEDKFAERVALLKPYQVNMELVKKAENENLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGDPFV
                     PRV"
     gene            complement(13739..14179)
                     /locus_tag="U1099_000866"
     CDS             complement(13739..14179)
                     /locus_tag="U1099_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867200.1"
                     /translation="MPSIERTTIGSEEWQRAASIYVRYKVFVLERGIAKEGEFDQNDT
                     EGRVYANLFIGEEPVSTGRFLPVGPGQARLTRIATLPNFRGKGYGKMIISSLEAYARE
                     AGFHQLNIHSELTAKTFYESVGYQATSEVYQEDGEWCQALTKFI"
     gene            complement(14179..15411)
                     /gene="arcA"
                     /locus_tag="U1099_000867"
     CDS             complement(14179..15411)
                     /gene="arcA"
                     /locus_tag="U1099_000867"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEM4867201.1"
                     /translation="MMSNHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGVEVLYLEKLAAESLVTPEIREQFIDEYLEEANIRGRA
                     TKKAIRKLLLSIKDNQELVEKTMAGIQKAELPEIPAEEKGLTDLVESSYPFAIDPMPN
                     LYFTRDPFATIGNAVSLNHMYSETRNRETLYGKYIFTHHPEYGGKVPLVYNREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFEQHVGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVFSVTYENDTLHIEEEHGDLAELLAANLGLEKVELI
                     RCGGGDMVAAGREQWNDGSNTLTIAPGVVVVYKRNTITNAILESKGLRLIKIGGSELV
                     RGRGGPRCMSMPFEREDI"
BASE COUNT         4547 a         3450 c         2804 g         4788 t
ORIGIN      
        1 gttatttact aactttgagc ccagttagtc ccacagaaca acataatgca ttccagaatg
       61 tttttacacc accccaaaat gaactaaacc atgatgattt ttgtacagaa ggggccattt
      121 gagtcgcagg tgagttgata ggctctaaat gttgaacacc ttgaccaaaa ggttgggttg
      181 attctttcag ctctagaagt acttcgtcac gctgctcaaa accaactggt gaattaagtc
      241 gttgctctgc atttacaatt gcaccacctg cagtagtcat caacaatgca agtgccatag
      301 cgcatgcgcc aactttataa ttacgttttg ttacaatatg tttcatgatg aaacatctcc
      361 tttatataga tatttgcttc ctcctatcgg aaacgtagta acagtatagc acaaaaaaat
      421 aactcaaaaa ccaactttag aaaaagatta tataaaaacc agaaaactac ctgaaaaaat
      481 tgaaaacgtt atacaaaacg tagaaaaaat gacactattt cgctcatttc acttgttcct
      541 atcaattttc aacatgataa aggatagcaa tatctcttgt tgaaattttc tctcacatcc
      601 aacttaatgt tatatataat ccattcaaat atataacaac tgatatggat tatcagaata
      661 gcaaaaggca tgcccttaaa aatataagtt acatgccttt tcacaatttt tgtcaaattc
      721 ataatggaaa aagaacctag attcattcag aaattatact tattgtactg ttacaaccaa
      781 ctttatgtct aagaataatc gtttacaaca tactacacta atttactcgc tgctgcttgg
      841 tcgaggatga ggatgacatt gtcatggttt tggaggacgc ttgctgggac atcttctgtc
      901 actgcccctt ccacagtcgc cttaatggcg tcagcttttt cctgaccata ggccatgaga
      961 acaatggtct tggctgccat aatgttggcg atgcccatgg agatagcttg ttttggtaca
     1021 tcttctggat tgtcaaagaa gcgggcgttg gcttcaatgg ttgatggcgc caattcaacc
     1081 agatgagtct gaccatcaaa tggagcacct ggttcgttga aaccgatatg gccgttgcga
     1141 ccgataccca aaatttggaa atcaaccgga tgttcagcca aaattttatt gtaacgttct
     1201 gtttcagcaa ctacatctgt cgccaaacca ttcggtaaat aagatttctt aaacggtttg
     1261 tcattgaaaa gattttcctt cataaaatga atataggatt ggtcgctctc ctcacctaga
     1321 ccaacatact catccaagtt tacagatgtc acattcgaaa agtctaaatc tgactcacga
     1381 atcaatctgt aaaattctaa cggactggag cctgttgcca aacctaatac cttggcacct
     1441 ccgttcaatt tttctctcaa aatgtctaat gctacagttg ctccttcaac ttgattgttt
     1501 actacatgaa ttttcataac acacctcttt ggtctatacc atttattgtt tttctttatt
     1561 atatggtata gaccaatttt tgtcaagaat ttatacctat agagtattac atcagcacga
     1621 ataattttaa ttctcttccc atccatcaag acttaagtgt ctaactatga tataatacat
     1681 ttatgacaga tgtgcttttt tacttatttt ttattggtat tctgttctgc cttactggct
     1741 attttatttc taaatcaaag gtcctaaagt ttattttcta cctgattggt agtctattgg
     1801 tggctttgcc atttgcccta ttgatttatt ttacctatat tttattttag tctaaggagt
     1861 gaatatgaat actgcagatt ttgattttga tttacccgaa gagcttatcg ctcaagttcc
     1921 ccttgaaaag cgcgacagct ctaaactatt gatcctcgac cgcgaaaaac agagcatggt
     1981 tgacagtcac tttgaccata tcatcgacca gttgaatccc ggtgatgccc tggtcatgaa
     2041 caatacacgt gttctgcctg cccgtctcta tggctacaag cctgaaacta tgggacatgt
     2101 tgagcttttg cttttgaaaa atacccaagg taatcagtgg gaagttcttg ccaaaccagc
     2161 aaaacgtctg aaggtcggta ccacagtcgc ttttggtgac ggacgattaa ctgctactat
     2221 cgtagaagaa ttagaacacg gtggtagaat tgttgaattc tcctatgaag gtatcttcct
     2281 tgaagtcttg gaaagtctag gcgaaatgcc acttccacct tacattcatg agaaattaga
     2341 agaccgcgaa cgctatcaaa cagtttatgc caaggaaaac ggttctgccg cagctccaac
     2401 tgctggacta catttcaccc aagagttact cgaaaaaatc gaagctaaag gagtgaaact
     2461 agtctatctg actcttcacg ttgggcttgg gaccttccga ccagtctctg ttgataacgt
     2521 tgatgagcat gaaatgcact cggaattcta taacctatca gcagaagctg ctcaaaccct
     2581 caatcaggtc aaagatagca gtggtcgtat cgtcgcagtc ggcacgacat ccatccgcac
     2641 cctcgaaacc atcggtaata aatttgatgg tcgtttggaa gctgattctg gttggacaaa
     2701 tatcttcatc aaacctggct acaccttccg aatcgttgat gccttctcaa ccaatttcca
     2761 cctgccaaaa tctactttgg ttatgctagt ttctgccttt gcaggtagag aatttacctt
     2821 agatgcctat aaacatgctg ttgaagaacg ctaccgcttc tttagttttg gggatgcaat
     2881 gttcattcaa taggctgtta tcaccaaatt attctgaatt ctgattttat tggagggccc
     2941 tatgaacaag attgaaagcc gccatcagct gatcctgtct ctaatcatgg agaagaaaat
     3001 acatacccaa caggaattgc aagaattact agagatcaac ggagtctccg tcacccaatc
     3061 taccctctcc cgtgacatca agatgctcaa tcttgtcaaa gtcaacgaag atgattcttc
     3121 tcactatgtc atcaacccca ttgctcctac tcgctgggaa aaacgactgc gactctatat
     3181 ggaagatgct ttggtcatgc taaaacctat ccaacatcaa gtggtgctca agaccctacc
     3241 tggattggcc aactcattcg gttctatttt ggatgccatg gaaattcctc aaattgtagc
     3301 gaccgtctgc ggtgacgatg tctgtctcat catttgtgaa gatgtcgagg gggcacaagc
     3361 ttgcttcgag cacttgaaac agtttactcc accatttttc tttagtaaat tataaaaatt
     3421 tatgttcaca aacaaatact taattttaaa gataattttt caaataaata tcaataactt
     3481 ccctttatac tcataaacga aaatctcccg taataagatt tattacgggg gatttttagt
     3541 agcctataaa aaattatcct gttattcata gccttctagt ttactttttg tacaaggcaa
     3601 tgagctgctt gctatactta ggtacggcaa agcgagtgaa agaaaaacta aatgactgat
     3661 atgtaattac aattagaaag ccctgaacat tcactcaaat ggctatatta cccctccttc
     3721 cgccggcgac tagcaaatgc cagaccaagc ataccaagga gagagaatcc aagaccatct
     3781 actgcgcctg tatttggcaa ttctttcttc tgagaagctg cttgctggga gcgtgactgc
     3841 tcactgcttg gttttgatac gagagataaa ggtgtgacag atggtccata gtttgatgtc
     3901 aggtatccct taaaggcagg gcgtggttct gcttggacac tttttccttt agcagtaatc
     3961 agaggtagct tgccttctgg taaggactct gctttgacgc tgtctccttt tgctgtcaca
     4021 ggaactaacg cttcacgcgc tgcttttact tctgccaagt aaccatcaac aaacgcttgg
     4081 ttatttctac ttagtttcca atcaatcttg ttcaagacct cttttaaagt cgccacgctc
     4141 tctggcgtat aggaatttaa atcagtagga atcttcccaa cttcttctcg caaatctcgg
     4201 tactctgcta aaaaataatc agaattgttt tccgtaaagc tgtgcaaaag ttttttaatt
     4261 gagtcatagc tgtaaggctt gcttgggtca tctgcccata ccgcaaccat gctaccgata
     4321 atgggtacct tagtattctc agatttcggt acttgatcaa accttgttga tttagttcct
     4381 cgaagacctt gatcaaggtt ataccatccc aaaccttctg cttcacgtcc aataacatag
     4441 taccaagcat catttgtatt aagaatttta tgcccttttt catgaaggga ttttgaggaa
     4501 gcaacatcat atcctcccca acctcctgtc cagaaagaag caataatgtc tttatcaaaa
     4561 gtacccgaac tagtatcgtg gttataatag ataccatcat tgaaagccat tggctctaaa
     4621 ccatgcttct tgacgatagc tgctaaatca ttggcatatt gaataaactt accataatca
     4681 ccttgtgtct gaagaatgtg ccatccagaa gcatttgtag catcgttagc atactcatct
     4741 aagccaatat tgaaaatttt agatttgcct gcaaagtaac tagcatattt atcaattaag
     4801 gctttagtaa aagcaactgc ttggtcattt tttaaatcaa ctgtacgtgc agatgttttg
     4861 ccattataac taaattttgg cgattttatc cccaattctt ccattgcttc tacaatcgca
     4921 tccatatgac ctgggctatt gatagttgga ataatggaaa tatttttttc cttagcataa
     4981 gcaagaatat catccatctc actctgactt aaggttgtac cgtttggatc gtcataataa
     5041 tgcttattac catttattat tgcttttcta actgcttcac tgtcatacac cttatctcca
     5101 acagtcaaat tcatatcatc taataaaaat ctcagaccat catttcccaa taagaggttc
     5161 atatccgtgt aaccataaga agaaattaaa tcaataatct cctttatctg atttacagag
     5221 aaatacttgc gaccggcatc gagtgaaaaa actttggaca aagctagttt ttcttgatca
     5281 acaataggtt tttctggttc tgcttcagga tgagtagcta caaaagttac ttttgctaac
     5341 tggacatcac tggcttgcag gcctttctct gttccatctt ccaacttata ggaaatacct
     5401 ttgaccgttg gaaagacgat cttcacaaat ttagtacctt ttcgaatgtt gcgaatatca
     5461 aatgtagtgc gtgcataagc tggggttgct tctacaaaat tatccactac agtaacattc
     5521 tctttattcc agcttttgcc gtcaacagat gaatacacag tgaagtcaat ttcaccgtct
     5581 tttgcattta tcaaatgttt gcgaacagat tttccttcat tagcccctgc ttctttatta
     5641 tagacagaat agtcttcaag tgttttgtaa taagtcgtta agtgcaaagc ctctggatta
     5701 gcaatcttgt aaactagtgc attttcgcca tcaccatcag catagagcct gcttgtttcg
     5761 taacgtggtt gacctccata gtagaaattg tttttgtttt catgagctgc tttcccgtta
     5821 gagactgtta cgaggtgacc aagtttttgc tgcggcttaa taacataatc attaggcttg
     5881 gcatcatcta gattatcaaa atgtacctga ctttgatctg ctttttgata aattcgaacc
     5941 caatcaacca agtactcact atcctgttta gtcatgttgc gtttgtaatc aacatcacca
     6001 acccagccat caccaacttg ggtttccagt attgggaaca tagggcgggt gtgcatacca
     6061 tccctatcct taccacggtc ggtttgaaaa acaaattttc catcaatata ccatttgatg
     6121 acatctggag tccattcgac ttcgtagaca tggaattcct gactccaagc ttcgtaattc
     6181 ttataagctg agccatcaga ttttttattt tttgccaaaa taccaaaatg gttagttgtc
     6241 catgcatccc atggattttg accaaggtat tccaaaacgt caatttcgtc atgcccggtc
     6301 tcatcctgtg ccaacatcca aatagcaggc cagattccct tagagtcatt taccttagct
     6361 cgaacagcca ttcgaccaaa ttggaaagaa tacttatctt tagattctag gcgaccagaa
     6421 gaccatgtta ctttatctgg taattcttta tcatcatacc gatcgtgccc aactgcttca
     6481 accaaagttt ttttatcctt atggttcgtg ctacggatag acaaatagct cttgtcgccg
     6541 tcttttttga tagaaactgc tttacgattg tatatagccg catgattcca catgccatca
     6601 ataatttgcc atttactttc atcaatcttg tcgccattaa attcatcaga ccagactaat
     6661 gtatcgtatt cagattttgg tggaatgtat tctggagcag attcgtcata agattttcct
     6721 cgtcccataa tctgaatttc tgaatagcga agaggagtat catctatgcg cgtacctcta
     6781 aagcgaacat aacgcgcctt agaatttttt ggagttttaa tcgttatgat ctgttccttg
     6841 tcaatggtga aattttcatt cttatagatt gtattataat tattaccatc attataatca
     6901 tcatccaaat ctatccggaa attacgatac tctccaggag ctaactgaac cttgatttcc
     6961 tcaatagtat agacatttct aaattcatat tgaagatagg cctggttccc tattgattct
     7021 tgagtagtat aattctcgtc gaactttcca tcgttaatgg cttctggatt agttggagcc
     7081 ttaccataga aatatggttt tttcaattta gcgaggttga ctagctcggt ttgtggttct
     7141 tcattcgtac cagcatctgc ttcaacctgc gccaagactt ctatctcgtt aaagagaacg
     7201 ccattactat atcccttcca tgaactgttt gtattttgga tataatgccc ccgtccccaa
     7261 atacgaatat aacgagcatt gattggtgct ggtaattgga taacttgagg ttgtccttta
     7321 tttgcaattg tttcttctac atctgcctgt ttgaaaatga cttctgtggt ttcaggagaa
     7381 aaatctacac ttgtagacaa ttccacttta acatctttaa aagtagagac tgcattatca
     7441 taagtattac gatgaatttt tacagtatgg acgggaactt ccttttcaaa atcatactga
     7501 acgtaaacat gatcccactt ggagtaaccg ttatctgatt cgccagtttc tttaccagca
     7561 atgattttgg tattgctatt ggcatcgtcg ctactaccgt aagcactccc atcggtagct
     7621 gcatctggat tttttataga aacagaaggg accgttgata attccgaaac catctgatgc
     7681 tggctgttag ttacgggctt tttcccttct accacatttt tatcagctac ttgtggttcc
     7741 tcctgttttt cttctggagt tgaaggttct gtttcagttg atggagtagc ttccacaagt
     7801 ggttttgctt caatagcagg tggagtttca ttgttgattg ctactggttc cccctgagat
     7861 acttcctctg ctgcaacggt ctgccctgcc cataccaaaa aactggtaca gatgagaagc
     7921 tgaccaagga tgactttttt cgtaaactta cttgtttctt ttttcattat ttcctcctat
     7981 aaagtctgca agtttatcct acctatagcc aaaataaagc agtttttaaa cctatttagt
     8041 ctcgtataag actcaaaatt cagtttactg gttgatgtgc gatcgcattt ctgacacaaa
     8101 tataacatgt aagcgtttta ttgtagaata gtctaacttg acttattcta tatgcagatg
     8161 ggttctattt ctaaaacagc atataataag tcacactttc atatattttt gtatacttat
     8221 cgtaaatagt gacacatttc tatctcatct ttctaatgat attcttcata agaaaagccc
     8281 ttagatattt cactaagagc tttgaaactg taatttacta ttcaatactg ataatggcta
     8341 ttccgcagct aggcgataga ctgcctcagc ataaatatcc attgttttat ataagtcttc
     8401 caatggcatg cgttcatttt cctgatgctc tgtttgttct gtatctggga aacatgcacc
     8461 aaaggccaca cagtttggca tagtacgagc aaaggtagcc ccacctgacg agattggctc
     8521 gcttgttaaa tctcctgtct tatcttgata aacagacatg agcgtagata ccagctggct
     8581 gtctaatggc acatacaagg aagcaaggta gtcgtactct tcataggtca agccacatga
     8641 ttgcgctttg gcttgtaaag tagaaactag ggcttcttta tccgcagtga caggaatacg
     8701 aatgtccaag cgaatttctg atttttttgc gctaatggtt agacctgcaa tattgaaact
     8761 gagtgtccct gatggttcat cggtcacatc accaaaaagc ttaaatcctg tggcatcttc
     8821 accaactgca ttgacgataa agtccaaggc tggatgactt tcgaaatgtt ccaaagcctt
     8881 tgccaaacga acgatggcat tgaccccctc ggcagcatcc ttggcgtgcc gcgaaatacc
     8941 aagaaccgtt acttgatcat ctttggtttc ataatcaaag cctaattgat ctaattccgc
     9001 aataactccg gctaataaat gaccagaata gcttgccttg gcaggtacta cgttataggc
     9061 agttcccgct tcgatactca gacaagggtg acctggacca tgcaatttgg cctgtagcaa
     9121 tcctttttca gcataagtca aagggaagga agaatctggt gcaaagccca tagttgctac
     9181 ttcttccaac tgattgtagc ggttcataca ccgccataag gtctcttcat ctgttccaaa
     9241 aatgaaacga atccgtttgt taaattggac tcccgcatca agaagtgctt ttactgcaaa
     9301 taaggccgcc atcgaaggac ctttatcatc ctgaaccccg cgtccaataa tataatctcc
     9361 ctctaccaca gcctcaaaag gcggcgtctg ccattgacta agatcaccag caggtaccac
     9421 atctaagtgg caaaggatag ctaataattc ttcgccttgc ccaatttcag catagccgta
     9481 ataaccagca gggtctaaat aagttttaaa acccattttt tccgtcaaag ccaacgtgtg
     9541 ctccagaaca tcctgaatcg cttgaccaaa tggcgtatct gcctgatgtt cttgcaaaac
     9601 agaaggaaaa gcaaccagtt cttggatggc ttgaatacat gcctcctgat gcgatgactg
     9661 aataaaactt gttctcattg tgtccccttt caacataata agatatgata tattttctag
     9721 cactattcta tcataaaagg aaaaactgag ccaaatttta tctgtattcg tgataaaatc
     9781 tgacccagaa tttcgattag aagaatgtcg caatgaccaa aatagctatg gaggcaatca
     9841 tgatgcctac gattaactta cctacaaatt tataccaagt accaatttca acacgaccaa
     9901 gtgccaaggc tcctattaca atacctgatg ttggagccac caagttcaag agacctgatg
     9961 cagcttggaa agctgtgata accaagcttg ctggtacatt tacaaactca ccaagaggtg
    10021 ccatgatacc catagaagcc cctgcaagac ctgatgaaga tggaatcaag aaggacattg
    10081 gtaggtagaa gagataggtc aaaatgatga atacttgtga agataaacct tgcaatcctt
    10141 cttcacccat gtggagaata gtggcagtaa tcataccatc gttcatgatg acttgaatac
    10201 cacgcgcgat agcacaaatc aaagctacgc tgaaaatatc agccgcaccg aacatgaagg
    10261 attttaccaa acgttcttca ctcataccat aaactaaacc aactacaatc cctgcgatac
    10321 caaagagcat tgctccttct gggaagtacc aagtaccaag agcagctgtt gaagaaccaa
    10381 tcacttgacc gatgaacgga agaccaatca accaatcttt aaattgtgta aagatagtaa
    10441 cacccaaatc ttcccatgga ataaagctac aaatcatgag aacaaatgtt aatacaaata
    10501 cccagagaac acgtttttga gctggtgata attctgcatt cacttcttga gttgctgtta
    10561 cattaaagtg ttgcatgtcc gcttcacgtt gtttataaac caaggactta gtcggatcgt
    10621 ttttcacttt ctcagcatag ttggctacaa agagaacccc catcaagaga ataacaacga
    10681 agaagaccaa gcgccaaatc ataccgtccg caacacttac acctgctgca tcagaggcta
    10741 caaccgttgc aaatgggttt acagtagaag ccaaacagcc gatttgagaa cctagcaaga
    10801 tgatggctac accagtaata ctatcaaaac caaccgccat cataacagga acaaggagcg
    10861 ggaagaaagc cattgtttct tcacccatac cataggttga gcctcctaag gcaaacaaag
    10921 gcatgaggat gtaaatcaac attttttcac gacctttgaa gcgttttaca atagaagcaa
    10981 tccctacatc aagtgcacct gtttcgttaa ctacaccaag aaaggcgcca accatgatga
    11041 tgaagaatgc tacgttaatc gctgcttcag tcgcaccgtg accaagcatg gcacgaactg
    11101 gagccataaa aacatcgtaa atcccttgtg gattttgagc aaccttctca taggtaccag
    11161 ccaccaaacg tccggcttca tccacctgat attgaccagc tggaataatc catgtcatca
    11221 ctgccataaa ggcaataatg agcatcaata ttgtataaga cgaaggcaat ttaaagcctt
    11281 ttttcacttt ttcgctcatc gcaaaaacct cctataatat tttaaaagtt tatcccctat
    11341 ttaagggaaa ttgtatccgt aataaccgta tcaaataccc acctatcttc aagagatgtc
    11401 tatctccttc agtctattat ctgaaatcat tgcttataga tagatacttg ataggaaatg
    11461 gatgagaggg gagggagctt agaagcccag accttcatgt ttagattaaa gggaaccctt
    11521 gtgggactat caacactttc actcccctaa ctccatcaat aaataacccg tctagtttaa
    11581 aaactatatt tgcatgcaga gtacatcaat aaatagaagc ctcaacgata catacagctg
    11641 cttagccttt gacaatgata gtgccgctat cggattcaat caaagcacct aagttttcta
    11701 atgaggtaat gactgctttt gattgcggct tattgtttac aaaagcaata gctgcttcaa
    11761 ctttcggtag catggaacct ggtgcgaatt gattttgttt gatatattct tccaattcag
    11821 ccacagtaac ggtttctaat ttttcttggt ctggtttgtt gtagttaaca aagacatagt
    11881 ccacacctgt caagacgata aacaagtctg catcaaccaa ttctgccaaa cattgagaag
    11941 caaagtcctt atcgataact gcttccacac cggtaagagt accgtctgct tcttggacaa
    12001 ctggaatacc tccgcctcca gcagctacga ctacctcacc tgcattgaga agggtacgaa
    12061 tggtaccaat ttctttgatt cctacaggtt ttggcgaagc aacgactttg cgccagccac
    12121 gaccggcatc ttctttgaag gttgcaccag tcttttctgc ttctgctttc gcttcttctt
    12181 ctgtatagaa tggtccaatc ggcttggtca aattttcgaa ggcaggatca ttcttatcca
    12241 cgataacctg ggtcacaaca gatgctacat ctttttcaat gccttctttg tgcaattcat
    12301 tttccaaggc attttgcaac cagaaaccga tcgaaccttc tgtcatggct accaaactgt
    12361 ctagtggaaa agctgggttc ttttccgaat cagcagcaag gttttgaaga agtaagtttc
    12421 caacctgagg accattacca tgcgtgataa taaggtcgtc cccatttttt atcaatttta
    12481 caaggtgctt ggctgtttgt accaaggcct ctttttgtgc tttagccgat gggtcggacg
    12541 ataaaatggc attgccaccc aaggctacta caattttacg atttgccata tctcttgctt
    12601 tttcctcact ttcgcgaaat agtaatcgct tacacatcta tcctcaaaat atgggggctg
    12661 ggtgccagcc cccggaggtt gcgatgcgtt attagatcgt taattattac acacgtggaa
    12721 caaatggatc ccctaaagta gccgccatca ctgctttaat agtgtgcata cggttttcag
    12781 cttggtcgaa gtgacgagca tatttgctac ggaatacttc gtctgttact tccatttctt
    12841 ctacgccgaa tttttcagcg acatctttac cataaacagt gtttgtatca tggaaggcag
    12901 gcaagcagtg caagaagatg agattttcat tttctgcttt tttcaccaat tccatattca
    12961 cttggtatgg tttcaagagg gcaacgcgtt ctgcaaattt atcttcttca cccattgata
    13021 cccaaacgtc agtataaaga acatctgcac ctttgactgc ttcgtcagca ttatcagtaa
    13081 tgagaacacg tgctccactt tctttcgcaa aaacttcagc caaggcaact acttcttctt
    13141 ctgggaagag ttcttttgga gagaagatat ggacattgac accaaggata gcacctgtca
    13201 ccaagagaga gttggcaacg ttgttacgac catcaccaca gtaaaccaga gtcaaacctt
    13261 ccaacttgcc aaagttttct ttgacagtca agtagtccgc caacatttga gttgggtgcc
    13321 atgcatctgt caaaccattc catactggca caccagagaa ttctgccaat tcttccacca
    13381 ttttttgact gaaaccacgg aattcaatac cgtcgaacat acgccccaaa actttagcag
    13441 tatcttctgt agattctttc ttaccaagtt ggatatcatt tgcaccaaga tattctggat
    13501 gggcaccaag gtcaatagat gctactgtga aggcagcacg agtacgtgtt gatgtttttt
    13561 caaacaagag ggcaatattt ttaccttcca aataacggtg tggaatattg cgttttttca
    13621 aatctttcaa atgagctgag aaatcaatca accattccaa ttctgcgcgt gtgaaatctt
    13681 tctctgcaag gaaatgtcta cctttaaata cgtttgtcat tgtctcttct cctaatgttt
    13741 aaataaattt tgtcaatgcc tggcaccatt ctccatcttc ttgataaact tcagaagttg
    13801 cttggtagcc aactgactca taaaaagtct ttgcagttaa ctccgaatgt atattcaact
    13861 gatgaaagcc tgcttcacgt gcataagctt ccaaagagct gataatcatt tttccatagc
    13921 cttttccacg aaaattcggt agagtggcta ttcgggttag acgagcttga ccaggaccga
    13981 caggaagaaa acgtcccgtc gaaacaggct cttcaccaat aaagagattg gcgtaaactc
    14041 ttccttccgt atcattctgg tcaaattcac cttcctttgc aataccacgc tcaaggacaa
    14101 ataccttata gcggacatag atagaggctg cgcgttgcca ttcttccgaa ccgattgttg
    14161 ttcgttctat acttggcatt agatgtcttc ccgttcaaac ggcattgaca tacaacgagg
    14221 tccaccacga ccgcgaacca attcgcttcc gccaatctta atcaaacgaa gccctttgga
    14281 ctcgaggata gcattggtaa tcgtattgcg tttgtaaaca actaccacac ctggtgcaat
    14341 agtcaatgta tttgaaccat cattccattg ctcacgacca gcagcaacca tatcaccgcc
    14401 accacaacgg atcaattcta ctttttcaag accaaggttc gcagccaaaa gttctgccaa
    14461 atcaccatgt tcttcttcaa tatgaagcgt gtcattttca taggttacag agaagacacg
    14521 aaggtcacct tcgatttctg ggtggatagt aaacttatca tagtcaacca tggtaaatac
    14581 cgtatccaaa tgcatgaatt tacggttgtt agcaaattcg aatgccaata ctttcttgaa
    14641 gccaacatgc tgttcaaaga tatttaccaa tagtttttca attgatgctg cgtcagtacg
    14701 ttgtgaaata cctactgcca aaacatcttt tgaaagaacc aattcatccc caccctcgat
    14761 acgagtagtt tcttcacgat tgtaaaccaa tggaactttt ccaccatatt ctgggtgatg
    14821 agtgaagatg tatttaccat acaaggtttc acggttccgt gtttctgaat acatgtggtt
    14881 aagagaaaca gcattaccaa tcgtagcaaa tggatcccgt gtgaagtaaa ggtttggcat
    14941 tgggtctatt gcaaatgggt aagaggattc aaccaaatct gtcaaacctt tttcttcagc
    15001 agggatttct ggcaattctg ccttttgaat accggccatc gttttctcaa ccagttcttg
    15061 attatcttta atagacaaca acaacttgcg aattgccttc ttagtcgccc gaccacgaat
    15121 gttagcttct tcgagatatt catcaataaa ttgttcacga atttcaggtg taacaagtga
    15181 ctcagcagct aatttttcca agtagagcac ttctacacct tcatcacgaa gagcttgagc
    15241 aaaggcatca tgctctttct gtgcatcttc taagaatgga atatcatcga agagtaggcg
    15301 ttctaggtag tcaggcataa ggttttcaat ttccttacct ggtctgtgta acataacttt
    15361 tttcagctta ccaatttctg aaaaaacatg aattggatgg tttgacatga tctgtcctcc
    15421 ttgtagattt atacatgatg ggaaacgagg aggaccgtac gctttcctgc ctctcatttc
    15481 ctctttacat ccatagttta acagagtaaa aaaacaaaaa cttgaaaacg ctcacattta
    15541 ttttgagaaa ttcttcacat ttttaacctt atttataaat aattatcga
//
LOCUS       DASFSW010000037        15157 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.037, whole genome shotgun sequence.
ACCESSION   DASFSW010000037 DASFSW010000000
VERSION     DASFSW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15157
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.037"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            505..675
                     /locus_tag="U1099_000868"
     CDS             505..675
                     /locus_tag="U1099_000868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867202.1"
                     /translation="MVVAEIHLLKNDQISVAINQKKPQLLLVENQAMSKQCSPDTIVI
                     KDSFSILFGTIK"
     gene            complement(1130..1768)
                     /locus_tag="U1099_000869"
     CDS             complement(1130..1768)
                     /locus_tag="U1099_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM4867203.1"
                     /translation="MIIHKSVNPVAFQNTYYLENDTHLIIIDPGSDWTKIQVTIEKIG
                     KPISAILLTHTHYDHIMSLDILRETYNFPPVYVAESEASWLYTPEMNLSGLPRHDDME
                     NVVCRPAEKIFQYQEDYQFDGFHFKVVPTPGHSIGGVSFIFPEDECVITGDALFRETV
                     GRTDLPTSNFDDLITGIREHIFTLPNHYTVHPGHGQNTSVGHEKNFNPFFRM"
     gene            1831..4344
                     /locus_tag="U1099_000870"
     CDS             1831..4344
                     /locus_tag="U1099_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEM4867204.1"
                     /translation="MEFVWSFFHEREYAIMNSMKDTRTSNRYAVVDLEATGTGTDAKI
                     IQIGIVLVENGEIIDSYATDINPYELLDDHIKNLTGITDQQLSQAPDFGQVASTIYDM
                     IGDAIFVAHNVKFDANLLAEALFFEGYELLTPRVDTVELSQLFFPTFEKYSLGNLAEH
                     LELGLDQAHTAISDAMATARLLIKIQEKIKSLPRSIVEKILDLADNLLFESRLVIDEM
                     VPVLSENLSYDLESIHDLVLKKPEEIKSAYRLSEDFSTNIALLGLHERKKQTAFAQVV
                     EKRLVDEEKVHFIQAQAGLGKTYGYLLPLLAKSDQPLLVTVPTKLLQEQIMEKEGSKL
                     KEIFRIPIVSLKSPKHFIKLDNYWKTLQRQDDNRLVNQFKMQVLVWLCETTTGDLDEL
                     KQKQRYQAYFDEIAHDGKLEPESLFWGWDFWQRLNHQALYSRLLITNHAYFLNHLKDQ
                     DPLMDKRLVAIDEAQKFLLAAENLATASQDLTSMLQVLQSKKDRATTILDQRLYESCQ
                     FELNHFLSRFRQHGQREVQKAELHQLGQNLEELGDVDLNDLHQLIDYYDTFWLEEKNL
                     EEKRLAYLRGSKDSLLNATNYLPDTKIFCISATLTISKKVSLADLLGFEQVTLDLIPT
                     QTVTNQQLLFPTHLPDILAWTKEEHAAYLATILGEIAELGRPILVLFTSISMLLQVSD
                     LLEDNNIPHLAQHKHGQEMTLKRKFERGECQILLGTGVFWEGVDFASQNQLIQVITRL
                     PFDNPKDRFVQKINHHLREEGKNPFYDYSLPMMMIKLKQAIGRTNRHDKQDSLVLVLD
                     PRVHTKRYGQQILSFFEKDYSMLSVDAKEIEGAIQDFFE"
     gene            4506..5582
                     /locus_tag="U1099_000871"
     CDS             4506..5582
                     /locus_tag="U1099_000871"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEM4867205.1"
                     /translation="MTEKTIVFKVGTSSLTQENGSLDRIKIARITNQLAQLHQKGYQI
                     VLVTSGSIAAGFRRLGFDKRPTKIAEKQASAAVGQGLLIEEYTQNLMKDGIVSAQILL
                     TQDDFADARRYQNASQALQVLLKQRAIPIINENDTIAIEEIKVGDNDTLSAQVASLLK
                     ADLLVLLTDVDGLYTANPNSDPTAQHLPEIKEITEDLFAMAAGAGSSNGTGGMTTKLQ
                     AAQIATKSGVPVFICSSKEDTALLQAVTQANRGTLFLADDHAMNQRKQWMAFYARTDA
                     AVEVDAGAVDAMLHQGRSLLATGVKALEGDFEVGQVVEVYSQADHRLIGKGRVKLSSN
                     DLQDQLANGRAEGVFIHRNDWVSL"
     gene            5647..6885
                     /locus_tag="U1099_000872"
     CDS             5647..6885
                     /locus_tag="U1099_000872"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEM4867206.1"
                     /translation="MTTTQVLLDSLLVHKVSINLATTEQKNQALSAMADQLVAQTEAI
                     LAGNAIDMEHAQGKISQVMQDRLLLTEERIEAMADGIRALIGLPDPVGLVLEESTRAD
                     GLNICKKSIPFGLVGMIYESRPNVTSDAAALAIKSGNAVILRGGKEAFHSAQAIVTAL
                     KAGLEQAGLSPKVIELVQDTSRASATELMTAKGKIDLLVPRGGAGLIQAVVENATVPV
                     IETGTGICHVYVDKDADLDKALRIVVNAKTSRPSVCNAAEVLLVHEEIASQFLPRLEE
                     ALSGQVELRADSQAQALLNQARPAGEQDFDTEFLDYVLAVKVVSNVEEAISHIAQHST
                     GHSEAIVTENSQTAEHFTLYVDSAAVYVNASTRFTDGGEFGLGCELGISTQKMHARGP
                     MGLREMTTYKYIITGDGHIR"
     gene            6895..7680
                     /gene="proC"
                     /locus_tag="U1099_000873"
     CDS             6895..7680
                     /gene="proC"
                     /locus_tag="U1099_000873"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEM4867207.1"
                     /translation="MKIGFIGLGNMGAALAHAVNQLPETQLLLSNHNPAKAQALQEKV
                     GGQLFSNGEIAEQAEVIFLGVKPHLIQTVLSGLQDQISQNPSAIWISMAAGVTLDSLA
                     AFVSADKLIRIMPNTPVAIGQGMTTYSLVNQELAPLLEQILEKSGKVQQVPESLIDAA
                     TAIAGCGPAFVYQMIEALTDAGVENGLTAQDAKVLAAQTLAGTAQMVLTSDKHPAQLR
                     QEVTSPGGSTIAGVVALEKSGFRYAIIRAVAAALKKTRKLGKK"
     gene            complement(7703..8107)
                     /locus_tag="U1099_000874"
     CDS             complement(7703..8107)
                     /locus_tag="U1099_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM4867208.1"
                     /translation="MAGRKISPQSLKNLYQSNKEANQLTKESIETALLFLLEKKELKQ
                     ISVSELVRKAGVSRNAFYRNYKSKEEILEDYYERTSNNLKKKWHDLQDKVQKDGVKQS
                     FADFVQEQKRKAEQSKALSNVSQWIKEKTKRD"
     gene            8234..9085
                     /locus_tag="U1099_000875"
     CDS             8234..9085
                     /locus_tag="U1099_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM4867209.1"
                     /translation="MSKWKIVADSGCDYRQLANLAPDTEFISVPLTIQVGEQAFVDDA
                     NLDIDHMMEVMEASKSAAGSACPSPQAYQAAFEGAENIIVVTITGGLSGSFNAARVAR
                     DMYIEEHPNVNIHLIDSLSASGEMDLLVDEINRLIGAGLDFSQVVEAITHYREHSKLL
                     FVLAKVDNLVKNGRLSKLVGTVVGLLNIRMVGEASAEGKLELLQKARGHKKSVTAAFE
                     EMKKAGYDGGRIVMAHRNNAKFFQQFSELVKASFPTAVIDEVATSGLCSFYAEEGGLL
                     MGYEVKA"
     gene            complement(9184..10443)
                     /locus_tag="U1099_000876"
     CDS             complement(9184..10443)
                     /locus_tag="U1099_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEM4867210.1"
                     /translation="MKKYQVIIQDILTGIEEHRFKRGEKLPSIRQLREQYHCSKDTVQ
                     KAMLELKYQNKIYAVEKSGYYILEDRDFQDHTVELNPADFQELPYEDFRICLNESLIG
                     RENYLFNYYHQQEGLAELISSVQSLLMDYHVYTKKDQLVITAGSQQALYILTQMETLA
                     GKTEILIENPTYSRMIELIRHQGIPYQTIERDLDGIDLEELESIFQTGKIKFFYTIPR
                     LHNPLGSTYDIATKTAIVKLAKQYDVYIIEDDYLADFDSSHSLPLHYLDTDNRVIYIK
                     SFTPTLFPALRIGAISLPNQLRDTFIKHKSLIDYDTNLIMQKALSLYIDNGMFARNTQ
                     HLHHIYHAQWNKIKDCLEKYALNIPYRISKGSVTFQLSKGILSPSIQHMFEKCYYFSG
                     QKADFLQIFFEQDFADKLEQFVRYLNE"
     gene            10565..11299
                     /gene="yaaA"
                     /locus_tag="U1099_000877"
     CDS             10565..11299
                     /gene="yaaA"
                     /locus_tag="U1099_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEM4867211.1"
                     /translation="MKIIIPNAKEVNTNLENASFYLLSDRSKPVLDAISQFDVKKMAA
                     FYKLNEAKAELEADRWYRIRTGQAKTYPAWQLYDGLMYRYMDRRGIDSKEENYLRDHV
                     RVATALYGLIHPFEFISPHRLDFQGSLKIGNQSLKQYWRPYYDQEVGDDELILSLASS
                     EFEQVFSPQIQKRLVKILFMEEKAGQLKVHSTISKKGRGRLLSWLAKNNIQELSDIQD
                     FKVDGFEYCTSESTANQLTFIRSIKM"
     gene            11404..12849
                     /locus_tag="U1099_000878"
     CDS             11404..12849
                     /locus_tag="U1099_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM4867212.1"
                     /translation="MKKRSGRSKSSKFKLVNFALLGLYSITLCLFLVTMYRYNILDFR
                     YLNYIVTLLLVGVAVLAGLLMWRKKARIFPALLLVFSLVITSVGIYGMQEVVKFSTRL
                     NSNSTFSEYEMSILVPANSDITDVRQLTSILAPAEYDQDNITALLDDISKMESTQLAT
                     SPATSYLTAYQSMLNGESQAMVFNGVFTNILENEDPGFSSKVKKIYSFKVTQTVETAT
                     KQVSGDSFNIYISGIDAYGPISTVSRSDVNIIMTVNRATHKILLTTTPRDSYVAIADG
                     GQNQYDKLTHAGIYGVNASVHTLENLYGIDISNYVRLNFISFLQLIDLVGGIDVYNDQ
                     EFTSLHGNYHFPVGQVHLNSDQALGFVRERYSLTGGDNDRGKNQEKVIAALIKKMSTP
                     ENLKNYQAILSGLEGSIQTDLSLETIMSLVNTQLESGTQFTVESQALTGTGRSDLSSY
                     AMPGSQLYMMEINQDSLEQSKAAIQSVLVEK"
     gene            12867..13556
                     /locus_tag="U1099_000879"
     CDS             12867..13556
                     /locus_tag="U1099_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEM4867213.1"
                     /translation="MNNQEVNAIEIDVLFLLKTIWRKKFLILLTAVLTAGLAFVYSSF
                     LVTPQYDSTTRIYVVSQNVEAGAGLTNQELQAGTYLAKDYREIILSQDVLTQVATELN
                     LKESLKEKISVSIPVDTRIVSISVRDADPNEAARIANSLRTFAVQKVVEVTKVSDVTT
                     LEEAVPAEEPTTPNTKRNILLGLLAGGILATGLVLVMEVLDDRVKRPQDIEEVMGLTL
                     LGIVPDSKKLK"
     gene            13566..14243
                     /locus_tag="U1099_000880"
     CDS             13566..14243
                     /locus_tag="U1099_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEM4867214.1"
                     /translation="MAMLEIARTKREGINKTEEYFNAIRTNIQLSGADIKVVGITSVK
                     SNEGKSTTAASLAIAYARSGYKTVLVDADIRNSVMPGFFKPITKITGLTDYLAGTTDL
                     SQGLCDTDIPNLTVIESGKVSPNPTALLQSKNFENLLATLRRYYDYVIVDCPPLGLVI
                     DAAIIAQKCDAMVAVVEAGNVKCSSLKKVKEQLEQTGTPFLGVILNKYDITTEKYSEY
                     GNYGKKA"
     gene            14282..15013
                     /locus_tag="U1099_000881"
     CDS             14282..15013
                     /locus_tag="U1099_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEM4867215.1"
                     /translation="MIDIHSHIIFGVDDGPKTIEESLSLISEAYRQGVRYIVATSHRR
                     KGMFETPEKIIMINFLQLKEAVAEVYPEIRLCYGAELYYSKDILSKLEKKKVPTLNGS
                     CYILLEFSTNTPWKEIQEAVNEMTLLGLTPVLAHIERYDALAFQSERVEELIDKGCYT
                     QVNSNHVLKPALIGERAKEFKKRTRYFLEQDLVHCVASDMHNLYSRPPFMREAYQLVK
                     KEYGEDRAKALFKKNPLLILKNQVQ"
BASE COUNT         4515 a         2894 c         3448 g         4300 t
ORIGIN      
        1 cttcgttttt cccactccct aggatctttt tattatatga cttttagtcc atcacaatcc
       61 gtaaagtatg tgacaaatga accgaggatg ggcaaaaaat ggtcctataa tttctgtagc
      121 gggtaaaacc actatggagc cttttaaatg taaaagccat atgacctata atgaaaagtg
      181 acaaaactta cattagaaag aggtcatata gaataaatca agagttaggg gagtgactct
      241 tgattcctga gcctagaaat agaaaagcga agatcttatc acaaattttc ttggaataga
      301 agacaaaaat atcgctatca tgactgaata tgatgtggaa actcacttag attgtgtgtc
      361 tccaaaatgt cttgcctgaa agggacaaat agtgaaatac aacttccaaa gagcatctaa
      421 aatcctttgc ttagaagcag ctgataatcc tctctcctta tccatcttag aaagcgtcgg
      481 ttcaagtgta aaggatgcgg gaaaatggtt gtcgctgaaa ttcacttatt aaaaaatgac
      541 caaatctctg ttgctattaa ccagaaaaaa ccccaattac tactcgttga gaatcaagca
      601 atgagtaagc aatgttcacc tgatactatt gtcatcaagg atagcttttc tatcctattc
      661 ggaactataa agtaagagtt tttgaagggg gtagatattg gttgcatgta ttgaaaaact
      721 tgtggttcca tggtttttaa agttactcta tctggctata ttttgaacta tgttattaca
      781 ccagtttctg attatgtagt gaattgcttg ttggaaggct atcagtaatc tgtgatttta
      841 gcagatttag tcaggagttt aaagacaact tggaacagaa ggatagaaca tggaaggagt
      901 taattagtat aacaaatgga gattattagc catgaggcga accattgaaa ctcgttttct
      961 gaatcatacg ctttgtttga tatagaacaa acctttgcca gaggactgct cggattacag
     1021 ctacggattg aacaaatcct acttatctac aatctaagct attttgactt taactagtag
     1081 cacgggtaaa aaaactgcct agttcaatca gaactgggca gtacttattt tacattctaa
     1141 aaaatggatt gaaatttttc tcatgtccaa cgctggtatt ttgaccatga cctggatgga
     1201 cagtatagtg atttggcagg gtaaagatat gttctctgat gccagtaatc aggtcatcaa
     1261 agttgctggt tggcaggtct gtgcgtccca cagtttcacg gaaaagtgcg tctccagtga
     1321 tgacacattc atcctctggg aagataaagg aaacaccacc aatagagtga ccaggtgttg
     1381 gaactacctt gaaatgaaaa ccgtcaaact ggtagtcttc ttggtattgg aagatttttt
     1441 cagcaggtcg gcagactaca ttttccatat catcatgacg aggtagacct gatagattca
     1501 tctctggtgt gtagagccag ctggcttcac tttcagccac atagacaggc gggaaattgt
     1561 aggtctcacg caggatgtcc aaactcataa tatggtcata gtgagtgtga gtcaggagaa
     1621 tagccgaaat tggttttccg attttttcga tagttacttg aatttttgtc cagtcactcc
     1681 cagggtcaat gataatcaga tgggtatcat tttcaaggta gtaggtattt tgaaaagcga
     1741 caggattgac agatttgtga ataatcattc tttttccttc ttttaacagt tactgatgtt
     1801 actagtctaa caaaaaaaca cgccttcttc atggaatttg tttggagctt tttccatgag
     1861 agggaatatg ctataatgaa cagtatgaaa gacacaagaa caagcaatcg gtatgccgtt
     1921 gtagatttgg aagcaactgg tacaggtacc gatgcaaaaa tcattcaaat cgggattgtt
     1981 ctcgttgaaa atggagaaat cattgacagt tacgcaacgg acatcaatcc ctatgagcta
     2041 ctggatgacc atatcaagaa tctgacaggt atcacagacc aacagctaag tcaggctcca
     2101 gattttggtc aggtagcaag taccatttac gacatgattg gagatgctat ttttgtcgcc
     2161 cataatgtca agtttgatgc taatctcttg gcagaagcac tcttttttga aggctatgaa
     2221 ctgttgacac cgcgtgtaga tacggtagaa ttatcacagc tattttttcc gacttttgaa
     2281 aaatatagct tgggaaacct tgcagagcat ttggaacttg gtttggacca ggcacacacg
     2341 gccatatcag atgccatggc tacggcgcgc ttgctcatca aaattcaaga gaaaataaag
     2401 agcttaccca ggtctatcgt tgaaaagatt cttgatttgg cggacaatct gctctttgaa
     2461 tcccgtctgg tcattgatga gatggtgcct gttctgtcag aaaatctatc atacgattta
     2521 gaatctattc atgacttggt cttgaaaaag ccagaagaaa tcaagtcagc ctaccgatta
     2581 tctgaagatt tttcgaccaa tattgccctt ctcggcttgc atgaacgaaa aaaacaaact
     2641 gcttttgctc aagtagtaga aaaacgcttg gttgacgaag aaaaagtgca ttttatccaa
     2701 gcgcaggcag gtttggggaa gacttacggt tatctcttgc ctttacttgc taagtcagac
     2761 caacctttat tggtcaccgt accgaccaag ctccttcaag agcagattat ggaaaaggaa
     2821 gggagcaagc ttaaagagat atttcgtatt cctatagtct ctctaaaatc gcccaagcat
     2881 tttatcaagt tagacaacta ctggaaaacc ctgcagaggc aggatgataa tcgcctggtc
     2941 aatcaattca aaatgcaggt gcttgtctgg ttatgcgaaa ccacgacagg tgatttggat
     3001 gagcttaagc aaaaacaacg ctatcaagct tactttgatg agattgccca cgatgggaaa
     3061 ttggagcctg aaagtctttt ttggggctgg gatttttggc aacgattgaa ccaccaagct
     3121 ctctatagta gactgctcat taccaaccac gcttattttc tcaaccatct taaggatcaa
     3181 gaccctctca tggataagcg tctggtggcg attgatgaag cacaaaagtt tctcttggct
     3241 gctgaaaatc tagcgacggc ttcgcaagat ttgacaagta tgttacaagt attgcaaagt
     3301 aaaaaggaca gagcgacgac tattttggac caacgtttgt atgagtcttg ccagtttgaa
     3361 ctcaaccact ttttgagtcg ttttcgccag catggtcaac gagaggttca aaaggctgaa
     3421 cttcatcaac ttggtcagaa cctggaggag cttggagatg ttgatttgaa cgacttgcat
     3481 cagctcatag actattacga taccttttgg ttggaggaga aaaatttaga agaaaagcgg
     3541 cttgcctatt tgcggggctc caaggacagc ctcttaaatg cgaccaacta cctgccagat
     3601 actaagattt tctgcatcag cgcaaccttg accattagca aaaaggtcag cctggcggat
     3661 ctgctaggct ttgagcaggt caccttggat ttgataccga ctcaaactgt gaccaaccag
     3721 cagctgcttt ttccgaccca tctccccgac atcctcgctt ggaccaagga ggagcatgca
     3781 gcttatttgg ctactatttt aggagaaata gcagagctgg gacgaccgat tttggtcttg
     3841 tttacatcga tttctatgct attgcaggtg tcggatttgt tagaagacaa taacattccc
     3901 cacctagccc agcacaagca tggacaggaa atgactctca aacgcaaatt tgaacgaggg
     3961 gaatgccaaa ttttactcgg cacaggtgta ttctgggagg gcgttgactt tgccagtcag
     4021 aatcaattga ttcaggttat tacaaggctc ccatttgaca atccaaagga ccggtttgta
     4081 caaaaaatca atcaccatct gcgtgaagaa gggaaaaatc cattttatga ttacagcctg
     4141 cctatgatga tgattaagct caaacaagcc atcggtcgta ccaatcgtca tgacaaacaa
     4201 gattcactag tcttggtttt ggacccgcgt gttcacacca aacgctatgg acagcaaatt
     4261 ctgtccttct ttgagaaaga ttattcaatg ttaagtgtgg atgcgaaaga aatagaagga
     4321 gctattcaag atttttttga ataagttgaa agggagccac aaggcttcct tttgtttcca
     4381 gcgttttttt aatgctactc atgattagga atctattccc tctctccttg ccgaataaca
     4441 gtgattttct ttatgattaa aatatggtat aattttccaa atattagttt ttgtgagaag
     4501 agattatgac agaaaaaaca attgtattta aggtcgggac aagttctctg acgcaagaaa
     4561 atgggagttt ggaccgtatc aaaatcgctc ggattaccaa tcagctggca cagttgcacc
     4621 agaagggtta tcagattgta ctagtaacat ctggttcgat cgctgctggt tttcggagat
     4681 tgggctttga caagcggccg actaagattg cggagaaaca ggcttctgcg gcggttggtc
     4741 agggcttgct gattgaggaa tatacgcaaa atctgatgaa agatggcatt gtgtcggctc
     4801 agattttgct gacccaggat gattttgcgg atgctagacg ttatcagaat gcctctcagg
     4861 ccttgcaggt cttgctgaag cagcgtgcta ttcctattat caatgaaaat gataccattg
     4921 ccattgagga gattaaggtg ggggataacg atactttatc tgcccaggtg gcttctctct
     4981 tgaaagcaga cctattggtg ctcttgacgg atgtagacgg gctttatact gccaatccca
     5041 acagcgatcc gactgcccag cacttgcctg aaatcaagga aattacggaa gatttgtttg
     5101 ccatggcagc aggagcaggc tcgtccaacg gaacaggtgg catgaccacc aaattgcagg
     5161 cagcccagat tgcgaccaag tcaggcgtgc ccgtctttat ctgctcttca aaagaggata
     5221 ccgccctctt acaggcggtc acccaagcca atcgtggaac gctcttttta gcagatgacc
     5281 atgccatgaa ccaacgcaag cagtggatgg ccttctatgc tcggacagac gcggcggttg
     5341 aagtggatgc aggagcggtt gatgccatgt tgcatcaggg gcgcagtttg ttagctactg
     5401 gtgtcaaagc cctcgaagga gactttgaag tgggccaggt cgtagaggtt tatagccaag
     5461 cagaccaccg tttgattggt aaaggacggg tcaaactgtc ctccaatgac ttgcaggacc
     5521 agttggcaaa tggcagagca gagggcgtgt tcattcaccg caacgattgg gtgagcttat
     5581 agttgttcag tttgtctcga ttatgttgac gaacgagcaa actgaaagac taaataggag
     5641 aaatttatga caacaacaca agtattatta gatagtttat tggtccacaa ggtttctatc
     5701 aacctagcga caacagaaca gaaaaaccag gccctatcag caatggcaga ccagttggtt
     5761 gctcagactg aggcaatcct agcaggcaat gccatcgaca tggaacatgc ccaaggcaag
     5821 attagccagg tgatgcagga ccgtttgctg ctgacagagg aacggattga agccatggca
     5881 gatggtattc gggccttgat tggcttgcca gatcctgtcg gattagtctt ggaagaatct
     5941 actcgggcag acggcttgaa catttgcaag aaatccattc cctttggttt ggtgggaatg
     6001 atttacgaaa gccgaccaaa cgtgacctca gacgccgctg ccttggccat caagagtggt
     6061 aatgcggtca ttttgcgtgg aggaaaggaa gcttttcatt cggcccaggc tattgtcaca
     6121 gccctcaaag ctggtttgga gcaggctggt ctttcaccta aggtcatcga gctagtccaa
     6181 gataccagtc gtgcctctgc gacagagttg atgaccgcaa aaggcaagat tgacctcttg
     6241 gtgccacgtg gcggtgcagg actcattcaa gccgtcgttg aaaatgcgac tgtgccagtt
     6301 atagaaacag gaacaggcat ctgccatgtc tatgtggaca aggatgcgga cttggacaag
     6361 gccttgcgga ttgtggtcaa tgccaaaacc agtcgtccct cagtctgcaa tgcggcggaa
     6421 gttttattgg ttcatgaaga aattgccagc caattcctac ctcgcttgga agaagctctt
     6481 tctggtcagg tagaattgcg tgccgacagt caggcccagg ctcttctcaa ccaagccaga
     6541 ccggcaggtg agcaggattt tgacacagag tttctggatt atgtcttggc ggtcaaggtc
     6601 gtttcaaatg tagaagaggc tatcagccac atcgctcaac actcgacagg gcacagcgag
     6661 gctatcgtaa cggaaaacag ccagacagca gagcatttca cactttatgt cgattccgca
     6721 gcagtctatg tcaatgcctc gacccgcttc acagacggtg gcgagttcgg tctgggttgt
     6781 gagctgggta tttccaccca gaagatgcac gcccgtggtc caatgggact gcgtgaaatg
     6841 accacttaca agtacatcat cacaggcgac ggccacattc gttaggagga caagatgaag
     6901 attggattta tcggactggg caatatggga gcggcattgg ctcatgcagt caaccagctg
     6961 ccagaaaccc agctcctcct cagcaaccat aacccagcaa aagcccaggc tcttcaagag
     7021 aaggtaggcg gtcagctttt ttctaatggg gaaatagcag agcaggccga ggtcattttc
     7081 cttggggtca agcctcacct gattcagaca gtcttgtcag gattgcagga ccagattagc
     7141 cagaatccgt cagctatttg gatttccatg gcagcgggtg tgacccttga cagcctggca
     7201 gcatttgtat cggcagacaa gctcatccgc atcatgccca acacaccagt cgctatcggc
     7261 caaggcatga cgacctatag cttggtcaat caagagctcg ctccgctatt ggaacaaatc
     7321 ctagaaaaat caggcaaggt ccaacaggtg ccagagagcc taatcgacgc agcaacggct
     7381 atcgcaggct gcggaccagc ctttgtctat cagatgattg aggctttgac cgatgcagga
     7441 gtggaaaacg ggctcacggc tcaggatgcc aaggtattgg cggctcagac cctggcaggt
     7501 accgctcaga tggtcttgac cagcgacaag cacccggccc agttgagaca ggaagtgacc
     7561 tcgccaggag gatcgaccat cgcaggtgtt gtagccctag aaaaatcagg cttccgctac
     7621 gccatcatca gggcagtcgc cgccgccctc aaaaagacta gaaaattagg caaaaaatag
     7681 aagacagcaa aaaacgggac ttttaatccc gttttgtttt ttccttgatc cattggctga
     7741 cgttggacag ggccttgctt tgctctgcct tgcgtttttg ttcctggaca aaatctgcaa
     7801 agctttgctt gacgccgtcc ttttgaacct tgtcctgcaa atcatgccat ttctttttaa
     7861 ggttgttaga agtccgttcg taatagtctt caaggatttc ttccttggac ttataattgc
     7921 gatagaaagc gttgcgagaa actcccgctt tacggaccaa ttcagatacc gaaatttgct
     7981 tgagctcctt tttctctagg agaaacagca gagcagtttc tattgattcc ttagtcagtt
     8041 gattcgcttc tttatttgat tgatagaggt ttttcaaaga ttggggtgaa attttacgac
     8101 ctgccatagt tttccttttc cgagtgtcac atctgaaaga aaatgctttc agatagggta
     8161 gtttaatgat ataattgtaa gtaaatagag atttattgtc aaataaaaaa gtaaaattgg
     8221 aaatgagaat attatgtcaa aatggaaaat tgttgcagat tcgggctgcg actatcgtca
     8281 attagctaat ctagctccgg acactgaatt tattagtgtg ccacttacca tccaagttgg
     8341 agaacaagca tttgttgatg atgcaaactt ggatattgat cacatgatgg aggtgatgga
     8401 agcatctaag tctgcagcgg ggtcggcgtg tccaagtccg caggcttatc aggcagcttt
     8461 tgagggagct gagaacatta tcgttgtgac gattacaggt gggttatcgg gtagttttaa
     8521 tgcggcacga gtagctaggg atatgtatat cgaagagcat ccgaatgtca atatccattt
     8581 gatagatagt ttgtcagcca gtggggaaat ggatttactt gttgacgaaa tcaatcgctt
     8641 aattggtgca ggactagatt tttcacaagt agtagaagca ataactcact atcgggaaca
     8701 cagtaagctc ctctttgttt tagcgaaggt tgataatctt gttaagaatg gaagactgag
     8761 caaattggta ggcactgtcg ttggtcttct caatatccgt atggttggtg aggcaagcgc
     8821 tgaaggaaaa ttagagttgc ttcaaaaggc gcgtggtcat aagaaatctg tgacagcagc
     8881 ctttgaagaa atgaaaaaag caggttatga tggtggtcga attgtcatgg cccaccgcaa
     8941 caatgctaag ttcttccaac aattctcaga gttggtaaaa gcaagttttc caacggctgt
     9001 tattgacgaa gttgcaacat caggtctatg cagtttttat gctgaagaag gtggactttt
     9061 gatgggctat gaagtgaaag cgtgattcac agagtaacaa ttttgggatg taatttccac
     9121 tatagaataa tccccctcgt cttctaagtt cgagggggat tgtttgtatg agactattgg
     9181 atttcattca ttcaaatatc ttacgaattg ctccagttta tctgcaaaat cttgttcaaa
     9241 gaagatctgt aagaaatcag ctttctgtcc gctgaaataa taacactttt caaacatgtg
     9301 ttggatgcta ggcgaaagaa tccccttgct tagctgaaag gtcacgctcc ctttggaaat
     9361 tcgatacggg atgttcaaag cgtatttctc taaacaatct tttattttat tccactgagc
     9421 gtgataaatg tgatgaagat gctgtgtgtt ccgtgcaaac ataccgttat caatgtagag
     9481 tgagagagct ttttgcatga taagattggt atcgtagtcg attagactct tatgtttgat
     9541 gaaggtatca cgtagctggt taggaaggct gattgcaccg attcggaggg caggaaagag
     9601 tgtcggtgta aaagatttta tatagatgac gcgattatct gtatcaagat agtgtaaagg
     9661 taggctatga ctagagtcga aatccgccaa atagtcatcc tcaatgatgt agacatcgta
     9721 ttgctttgct aattttacga tggctgtttt tgttgctata tcataggttg aaccgagagg
     9781 gttgtgcaag cgaggaattg tgtagaaaaa cttaattttt ccagtttgga agatactttc
     9841 caattcttct aggtcaattc catctaaatc ccgttcaatt gtttgatagg ggattccttg
     9901 atgtcgaatg agctctatca ttcgtgaata ggtagggttc tctatcaaga tttccgtttt
     9961 tccagccaag gtttccattt gtgtgagaat atatagagct tgttgactac cagctgtgat
    10021 aaccagttgg tctttttttg tatagacatg atagtccatt aacagacttt gaacggagga
    10081 aatcaattct gccaatccct cttgctggtg atagtagttg aataggtaat tttcccggcc
    10141 aataagactt tcgtttagac aaatccgaaa atcttcatag ggtaattctt gaaagtctgc
    10201 aggattgagc tctacagtat ggtcttggaa atctctatcc tctaagatat aataaccgct
    10261 tttttcgaca gcgtagatct tattttggta ttttaattcc aacatagcct tttggacagt
    10321 gtctttgcta caatgatatt gctcgcggag ttgacggata gaaggtaatt tctctccacg
    10381 tttgaatcga tgttcctcta ttccagtcaa aatatcttgg atgataactt gatatttttt
    10441 catctaggtc ccctttttat agactatgtt actagctagt atatagaaaa aattgaagaa
    10501 agacaatata tgaataatgg ggttgaggtt caggaattaa gctactctat ggtataatta
    10561 agtgatgaaa ataattatac ctaatgcaaa agaagtaaat acaaatctag agaatgcctc
    10621 gttttatctc ctgtctgatc gaagcaagcc ggtgctggat gccataagtc aatttgatgt
    10681 aaaaaagatg gctgcctttt ataaattgaa tgaagcaaag gctgagttag aagctgaccg
    10741 ttggtatcga atcaggacag gtcaagcaaa aacctatcca gcctggcagt tatatgatgg
    10801 tctcatgtat cgttatatgg ataggcgagg tatagattcg aaagaagaaa attatctacg
    10861 tgaccacgtt cgtgtagcga cagccttata cggattgatt catccttttg aattcatttc
    10921 acctcaccgc ttagattttc aagggagctt aaagataggc aatcagtctt tgaaacagta
    10981 ctggcgaccg tattatgacc aagaagttgg tgatgatgaa ctgattctct cactggcttc
    11041 gtcagaattt gagcaggtgt tttctccaca gattcagaaa agattagtta aaattctttt
    11101 catggaagaa aaagcaggtc agctaaaagt tcactcgact atatcaaaaa aaggcagagg
    11161 aagattgctg tcctggttgg ctaagaacaa tattcaggaa ttatcggaca ttcaagattt
    11221 taaggtggat ggctttgaat attgtacttc cgaatcaacg gcaaaccaac ttaccttcat
    11281 acgatcaata aaaatgtgaa attatgaaaa agataacgtt ttccagcgct aaaaagggta
    11341 gaaaaatatt aatttctatg atataatgga tgcgttatgg gtaaaagtct aggaaggttg
    11401 tttatgaaaa agagaagcgg acgaagtaag tcgtccaagt tcaaattggt aaattttgcg
    11461 cttttgggac tttattccat tactctatgt ttgttcttag tgaccatgta tcgctataac
    11521 atcctagatt tccggtattt aaactatatt gtgacgcttt tgctagtagg agtggcagta
    11581 ttggctggat tattgatgtg gcgtaagaaa gcgcgcatat ttccagcgct cttacttgtt
    11641 ttttcactgg tcatcacgtc tgttgggatc tatggaatgc aagaagttgt aaaattttca
    11701 acacgactaa attcaaattc gacattttca gaatatgaaa tgagtatcct tgtcccagca
    11761 aatagtgata ttacggacgt tcgtcagctt actagtatcc ttgctccagc cgaatacgac
    11821 caagataaca tcaccgcttt attggatgac atatccaaaa tggaatctac tcaactagca
    11881 actagccccg cgacctctta cctgacagca tatcaatcta tgttgaatgg cgagagtcaa
    11941 gcgatggtgt tcaacggagt ttttaccaat attttagaaa atgaagatcc aggcttttct
    12001 tcaaaagtga aaaaaatata tagtttcaaa gtgactcaga ctgttgaaac agctactaag
    12061 caggtgagtg gagatagctt taatatctat attagtggta ttgatgctta tggaccgatt
    12121 tctacggtct ctcgttcaga tgtcaatatc attatgactg tcaatcgtgc gacacataag
    12181 attttattga caactactcc acgagattca tacgttgcta tcgcagatgg cgggcaaaat
    12241 caatacgata aactaacaca tgctggtatc tacggtgtca atgcttctgt gcacacctta
    12301 gaaaatctct atgggattga cattagcaat tatgtgcggt tgaacttcat ttccttcctt
    12361 caattaatcg acttggtggg tggaattgat gtatataacg atcaagaatt tacaagttta
    12421 catgggaatt atcatttccc tgttggacaa gttcatttaa actcagacca agcattaggc
    12481 ttcgttcgag agcgctactc tttaacagga ggtgacaatg accgtggtaa aaaccaggaa
    12541 aaagtgattg ctgccttgat taaaaagatg agtacgccag agaatctaaa aaattaccag
    12601 gcaatcctat ctggattgga aggctcaatt caaacggatt tgagcttaga aacgattatg
    12661 agtttagtga atacccaact agaatcagga acacaattta cagtagagtc acaagcattg
    12721 acaggaacag gacgctcaga cttatcttct tatgcgatgc ctggatcaca actttatatg
    12781 atggaaatta accaagatag tctggagcaa tcaaaggcag cgattcagtc cgtacttgtt
    12841 gaaaaataaa gattttagga gaaaatatga acaatcaaga agtaaatgca atcgaaatcg
    12901 atgttttatt cttactaaaa acaatttgga gaaagaaatt tttaattctc ttaactgcag
    12961 tgttgactgc ggggttggca tttgtctaca gtagtttttt agtgacacct caatatgact
    13021 ccactacccg tatctatgta gtgagtcaaa atgttgaagc cggtgcgggc ttgactaacc
    13081 aagagttaca agcgggtacc tatttggcaa aagactatcg ggaaattatc ctatcacaag
    13141 atgtattgac acaagtagca acggaattga atctgaaaga gagtttgaaa gaaaaaatat
    13201 cagtttctat tcctgttgat actcgtatcg tttctatttc tgtgcgtgat gcggatccaa
    13261 atgaagcggc acgtattgca aatagccttc gcacctttgc agtgcaaaag gttgttgagg
    13321 tcaccaaggt aagcgatgtg acgacacttg aagaagcagt cccagcggaa gaaccaacca
    13381 ctccaaatac aaaacgaaat atcttgcttg gtttattagc tggaggtatc ttggcaacag
    13441 gtcttgtact ggttatggag gttttggatg accgtgtaaa acgtcctcag gacatcgaag
    13501 aggtaatggg attgacattg ctaggtatag taccagattc gaagaaatta aaataggaga
    13561 acaatatggc gatgttagaa attgcacgta caaaaagaga gggaataaat aaaaccgagg
    13621 agtatttcaa tgctatccgt accaatattc agcttagcgg agcagatatt aaggttgttg
    13681 gtattacctc tgttaaatcg aatgaaggta agagtacaac tgcggctagt ctcgctattg
    13741 cctatgctcg ttcaggttat aagaccgtct tggtggatgc agatatccga aattcagtca
    13801 tgcctggttt cttcaagcca attacaaaga ttacaggttt gacggattac ctagcaggga
    13861 caacagactt gtctcaagga ttatgcgata cagatattcc aaacttgacc gtaattgagt
    13921 caggaaaggt ttctcccaac cctactgccc ttttacaaag taagaatttt gaaaatctac
    13981 ttgcgactct tcgtcgctat tatgattatg ttatcgttga ctgtccacca ttaggactgg
    14041 taattgatgc agctatcatt gcacaaaaat gtgatgcgat ggttgcagta gtagaagcag
    14101 gcaatgttaa gtgctcatct ttgaaaaaag taaaagagca gttggaacaa acaggcacac
    14161 cgttcttagg cgttatcttg aacaaatatg atattaccac tgagaagtat agtgaatacg
    14221 gaaattacgg caaaaaagcc taatttctca gataacataa gtttgataag taggtattaa
    14281 tatgattgat atccattcgc atatcatatt tggtgtggat gacggtccca aaactattga
    14341 agagagcctg agtttgataa gcgaagctta tcgtcaaggt gttcgctata tcgtagcgac
    14401 atctcataga cgaaaaggga tgtttgaaac accagaaaaa atcatcatga ttaactttct
    14461 tcaacttaaa gaggcagtag cagaagttta tcctgaaata cgattgtgct atggtgctga
    14521 attgtattat agtaaagata tcttaagcaa acttgaaaaa aagaaagtac ccacacttaa
    14581 tggctcgtgc tatattctct tggagttcag tacgaatact ccttggaaag agattcaaga
    14641 agcagtgaac gaaatgacgc tacttgggct aactcccgta cttgcccata tagagcgtta
    14701 tgatgctctg gcatttcagt cagagagagt agaagagcta attgacaagg gatgctacac
    14761 tcaggtaaat agtaaccatg tgttgaagcc tgctttaatt ggcgaacgag caaaagaatt
    14821 taaaaaacgt actcgatatt ttttagagca ggatttagta cattgtgttg ctagcgatat
    14881 gcataattta tatagtagac ctccgtttat gagggaggcg tatcagcttg taaaaaaaga
    14941 gtatggtgag gatagagcga aggctttgtt caagaaaaat cctttgttga tattgaaaaa
    15001 tcaagtacag taacctcata gaaatagtgg aggagctatg aatattgaaa taggatatcg
    15061 ccaaacgaaa ttggcattgt ttgatatgat agcagttgcg atttctgcaa tcttaacaag
    15121 tcatatacca aatgctgatt taaatcgttc tggaatt
//
LOCUS       DASFSW010000038        15111 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.038, whole genome shotgun sequence.
ACCESSION   DASFSW010000038 DASFSW010000000
VERSION     DASFSW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15111
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.038"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            252..722
                     /locus_tag="U1099_000882"
     CDS             252..722
                     /locus_tag="U1099_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEM4867216.1"
                     /translation="MKISVKKEAGHYLLSICYSVSASLTEAWDLLATDSGFAHWFPQL
                     NIVGNTLVFEMEGFREVMDLLEYRKNEKIAYRWDSATVSFTLSQLENQTLITFEERIP
                     EDFGNEFANAQKDMTGWLVPNECIKKFLEGQEPPVCQPLQEKWRTFLELELEGL"
     gene            740..997
                     /locus_tag="U1099_000883"
     CDS             740..997
                     /locus_tag="U1099_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867217.1"
                     /translation="MKKWWEQLKEEYYETRFDSNEDWMEWFIKRKMTWGWRWPTAITI
                     FVLWALSLPDARNVVRFFYFLLTLGVATVLHKLISYLKRRK"
     gene            1004..2257
                     /locus_tag="U1099_000884"
     CDS             1004..2257
                     /locus_tag="U1099_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEM4867218.1"
                     /translation="MHQITCPHCGTAFQVNETEYSQLLAQVRGAEFDKEIHDRLERER
                     ELLSQKAENDLQARLSDKDKEILELTAKLDKFASQTELEISSAISQKDQEIQELKAQL
                     GQIGLAKDLELQQAVAQVEKERDAAQNALVLQEQKQELALATTRQEYEVRLKAADEQV
                     EFYKNFKAQQSTKAIGESLEQFAEAEFNKVRSYAFPHAKFAKDNEVSASGSKGDFIFR
                     DFDESGLEFISIMFEMKNEADTTKTKHKNADFFKELDKDRREKKCEYAVLVSMLEADN
                     DYYNTGIVDVSHEYEKMYVIRPQFFIQLIGILRNAALNSLQYQQELALVKEQNIDITH
                     FEEDLEIFKNAFAKNYQSASNNFQKAIDEIDKAIKRMEAVKAALTTSENQLRLANNKL
                     DDVSVKKLTRNNPTMKAKFEALREE"
     gene            2259..2753
                     /locus_tag="U1099_000885"
     CDS             2259..2753
                     /locus_tag="U1099_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867219.1"
                     /translation="MIVPLQFEHILQLDEAFQKQGWPSSKEILTNYLREQEKGERIVL
                     VAEETGVCKGYITLIKQVKEGPFYQSGIPEIADFNVFEDFQNQGIGWQLLDAICQLAS
                     EFTDIVGIGVGLNANYGKAQRLYVKHGFIPDGSGVWYRGRSLPVGAKAYNDDELALYF
                     TKKL"
     gene            2766..2996
                     /locus_tag="U1099_000886"
     CDS             2766..2996
                     /locus_tag="U1099_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEM4867220.1"
                     /translation="MNTIDLNLPVAEIINQHPEVKDILVELGFKPLANPAMLNTVGKV
                     TNLKTGSKMTKIPLDRIQQVLECNGYEVIGGE"
     gene            2996..4321
                     /locus_tag="U1099_000887"
     CDS             2996..4321
                     /locus_tag="U1099_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEM4867221.1"
                     /translation="MTEQRIDILKNILLDLHNGASPESVQDQFNQHFTGVSALEISMM
                     EHELMSSDTGITFEDVMNLCNIHANLFKGAIADVEVADMDQEGHPVYVFKQENLALRA
                     ALLRIRRIIDQHEQTEDAELKDQLLQGLTRQFGLLGQFENHYTRKEKVFFPIMERYGH
                     DAPPKVMWGVDDEIRDLFKTARKALEGGDLAATRAHFENFAKEFEEMIFKEEAILLMI
                     LLEIFTQDDWLQVAADSDAYGYAIVKPTAKWVPHRELFGEIAEQEVDSISPQPASPNQ
                     QIIDTPEGQFTITFTPKEKSETVQDRTSPQTFGNGYLSVEQANLILNHLPLELTFVNK
                     DDIFQYYNDSHPVEDMIFKRTPSQIGRHVELCHPPKIVDKVKKIFELLRTGQKDQVTM
                     WFKSESMGKFVYVVYKAVRDDQGEFQGVLEYVQDIQPFFEIDSDFHREI"
     gene            4352..4561
                     /locus_tag="U1099_000888"
     CDS             4352..4561
                     /locus_tag="U1099_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867222.1"
                     /translation="MDWPTIKIGDEGYYDAPLIFSYGYDEEAEEGIDEAVTFDAVPSE
                     TMRRIHKELENLIDTTFGSYRFYDD"
     gene            4614..5489
                     /gene="truB"
                     /locus_tag="U1099_000889"
     CDS             4614..5489
                     /gene="truB"
                     /locus_tag="U1099_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEM4867223.1"
                     /translation="MSGIINVKKEAGMTSHDVVFKLRKILQEKKIGHGGTLDPDVTGV
                     LPIAVGKATRMIEYMQEEGKIYEGEITLGFSTTTEDSSGDLVERTPVLDIAEQAVDEA
                     MTSFVGTITQIPPMYSAVKVNGRKLYEYARTGEEVERPQRQIEIYSFERTSPIELVDD
                     CARFTFRVRCGKGTYVRTLSVDLGAKLGYASHMSKLERTGSAGMDLADALTLEEISSL
                     VAENDFSFLQPIERGIGDLPVVELKEEQVQDATFGRFVELNAEAERLAGFHDGQLIAI
                     FEKRDQLYKPSKVLV"
     gene            5524..6447
                     /locus_tag="U1099_000890"
     CDS             5524..6447
                     /locus_tag="U1099_000890"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEM4867224.1"
                     /translation="MNIRTIKHYSELNQEEPTVLVLGYFDGIHLGHKALLDRARQVAD
                     EKGLSVTVLTFPETPRLAFSRFSPELLLHLTSQDQRYHLLEQYGVDQLVLTDFTSEFA
                     SNTPQQFLERYIKGLNAQVLVAGFDYHFGNCRADVTDLTELFDGQVEIVSEVSLGGEK
                     VSSTRIRQAIQSGEVSLANQLLGYPFMTEGIVVHGDARGRTIGYPTANLAPFDRVHLP
                     SEGVYVADVEVDGKRYRAMTSVGKNVTFDGTEMRIEAHIFGFHRFIYGEKITIFWLEK
                     IRDMVKFDGIEGLMEQMKSDEKFALQWGKYA"
     gene            6440..7186
                     /locus_tag="U1099_000891"
     CDS             6440..7186
                     /locus_tag="U1099_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEM4867225.1"
                     /translation="MLKRLIRHPFYEKAMTYIAVVYVFLIVLELMDASIANSSGYRVL
                     DLLLWFIFVCDYFAQLYYADDILEYVQGHILELIAIIPFDSFFSFLRLGRLARLFRLV
                     KVTRIFALSNRFWDTINKLLHTNSLSKVLMLNISAVLVSSVLLSVIEGKSFFDALWWS
                     IVTMTTVGYGDIVPQDTISKIIAILLMLVGICTFGMVTSTITRFFSETERETKLDILM
                     AKIDEQNETLLRLEKKIESLEKLGELQGRD"
     gene            7261..7662
                     /locus_tag="U1099_000892"
     CDS             7261..7662
                     /locus_tag="U1099_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEM4867226.1"
                     /translation="MITLFLSPSCTSCRKARAWLTSHEVPFIEHNIMTSPLTAEELKN
                     ILALTENGTDDLISTRSKVFQKLDIDVDELSVKQLIGLIETYPSLLRRPIILDEKRMQ
                     IGFNEDEIRAFLPRKYRKQELKNATLRAEIG"
     gene            7677..7955
                     /locus_tag="U1099_000893"
     CDS             7677..7955
                     /locus_tag="U1099_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEM4867227.1"
                     /translation="MNKNYSYPLDFSWSTEEISSVLSFLNQVEAAYEKGADAVAILAS
                     YKSFKDVVKSKGQERQIDRDFEAVSGYSTYRVVKAARDKGKGVIRFGN"
     gene            7945..8703
                     /locus_tag="U1099_000894"
     CDS             7945..8703
                     /locus_tag="U1099_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEM4867228.1"
                     /translation="METKYHFAQTIVLEAGNFLRQHLHDDLQIEEKGDFTDLVTHLDK
                     QVQEMLTQQIQSRYQGDGILGEEGGDVSSIHEGNVWVIDPIDGTTNFIAQKTDFAIMI
                     AYFENGVGKFGIIYDVMRDKLFHGGDSFPVYCNQERLKKPELRPLRQSLIGINAKLYA
                     GNAHGVAELANQSLGTRSVGSAGIGFTHVLEGRLFAYASYLCPWDYAAASIIGSSLGL
                     VMLSFEGPSLTYDKREFVMLVSESHLKEVKGYIF"
     gene            8703..10004
                     /locus_tag="U1099_000895"
     CDS             8703..10004
                     /locus_tag="U1099_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEM4867229.1"
                     /translation="MQLPQDFIDKYNHLLGSEAQAFFESFEQDPISGFRTNPLKESQL
                     DFEQPIPNMTWSYYGKVSGKSPEHVTGLVYSQEPAAQVVGQIAAPEKGMKVLDLAAAP
                     GGKSTHLLSYLENTGLLVSNEISNKRAKILAENIERFGARNVVVTNESADRLAKVFPG
                     YFDMIVLDAPCSGEGMFRKDPDAIQYWSKHYPAQCASLQREILESALNMLAPGGQLIY
                     STCTWSPEENEEVVTWLLEQYDLELIDIPKINGMVEGVGYPEVARMYPHHFAGEGQFV
                     AKFRFLGEVSQKKLKPGKSNLNREQQALWKEFEQKHLRVQLDGLLQVFGDNLYLLPHG
                     LPDLSKVKIARNGLHLGTYKKKRFEPSFALGLALHSSEVENRVEISLEEFSSYTAGHT
                     IAVSKDLANGWYQVAVQGNGLGFAKIVSGTLKNGFPKGLRF"
     gene            10141..11010
                     /locus_tag="U1099_000896"
     CDS             10141..11010
                     /locus_tag="U1099_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEM4867230.1"
                     /translation="MKKKQKLGIAALFLLSSMALSGCSSWIDRGESITAVGSTALQPL
                     VEAAADEFGSVNIGKSVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIVNEQVSVKDITTEDLRKIFTGEITNWKELGGKDLEISIINRAASSGS
                     RATFDSVIMDGQTATQSQEQDSNGMVKSIVAQTPGAISYLSFAYLDDSVKTIKLNGFE
                     PTAENVATNDWPIWSYEHMYTMGKATDLTEEFLDYMMSDEVQNGIVESMGYISINDMK
                     VVKSADGTVTEKE"
     gene            11013..11930
                     /gene="pstC"
                     /locus_tag="U1099_000897"
     CDS             11013..11930
                     /gene="pstC"
                     /locus_tag="U1099_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEM4867231.1"
                     /translation="MKNEQLAKELSSPSKNSRLEKFGKVITFLCLSLIVFIVAMILIF
                     VAQRGLSTFFVDGVSITDFLFGSKWEPSSKIFGALPMISGSFIVTILSAVVATPIAIG
                     AAVFMTEISPKRGAKILQPVIELLVGIPSVVYGFIGLQVVVPFVRSIFGGTGFGILSG
                     VFVLFVMILPTVTFMTVDSLRAVPRHYREASLAMGATRWQTIWRVILNAAKPGIFTAV
                     IFGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLLMSLIFNMIMKFITREGKKNYAR"
     gene            11920..12807
                     /gene="pstA"
                     /locus_tag="U1099_000898"
     CDS             11920..12807
                     /gene="pstA"
                     /locus_tag="U1099_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEM4867232.1"
                     /translation="MHAKKFDKLATGILYSIAGVIVVILTSLILYILLRGLPHVSWDF
                     LTSKSSSYKAGGGIGIQLYNSFFLLVVTLLISVPLSTGAGIYLAEYAKKGPLTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSILSGALALTVFNLPQMTRNVEDSLRHVH
                     HTQREAGLALGLSRWETVLHVVIPEALPGIVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATQVSAGSAAILLVFILIF
                     NLSARYIGKKLHAKLTSAA"
     gene            12832..13635
                     /gene="pstB"
                     /locus_tag="U1099_000899"
     CDS             12832..13635
                     /gene="pstB"
                     /locus_tag="U1099_000899"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM4867233.1"
                     /translation="MADYNWNERHIITFSEENIALSTKDVHVYYGKNEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIAKVTGQILYEGIDINRPDMNVYEIRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPQILLMDEPASALDPIATMQLEETMFELKKDYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTANVFQNAKLKSTSDYVSGHFG"
     gene            13647..14405
                     /gene="pstB"
                     /locus_tag="U1099_000900"
     CDS             13647..14405
                     /gene="pstB"
                     /locus_tag="U1099_000900"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM4867234.1"
                     /translation="MTSPILQVKDLSVYYNKKKALNNVSIDFYPNEITALIGPSGSGK
                     STLLRSINRMGDLNPEVTLTGAIDYNGKNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRINGIKDKQVLDEAVERSLIGASIWNEVKDRLHDSAVGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEDTLYGLKDKYTMVLVTRSMQQASRISDRTG
                     FFLDGDLIEYNLTKEMFLNPANKETEDYITGKFG"
     gene            14434..15090
                     /gene="phoU"
                     /locus_tag="U1099_000901"
     CDS             14434..15090
                     /gene="phoU"
                     /locus_tag="U1099_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEM4867235.1"
                     /translation="MLRAQFEEELGKLHNQFYAMGNEVLAQINNAVRAFTTHDRELAK
                     EVIEADKKINAFEVKLEKKSLEIIALQQPVSTDLRRVITVLKATSDLERMGDHAVSVA
                     KAAIRMKGEVRIPVVEEEIKKMGREVRQLVEETLELYLGDADMERAYEVAARDEVVNE
                     FYSTIQELTTEEIKKNPESLVAGRDYYQVLTYLERVGDYAKNICEWVVYLRTGDITEL
                     "
BASE COUNT         4493 a         2735 c         3539 g         4344 t
ORIGIN      
        1 acaatgtcca ttatgtgtta agaaagtcta acattgctag gggatacaaa acatggagta
       61 tgagccaggt aaacaaaggt aaggactgtc tatgaaagat gttataaaaa ataattcaga
      121 tttcaagata ccgtttttcg atcctctgga atgaatagca ttcatgctta aagaatagag
      181 acaatggaaa tatcggttat agaccggtgt tttttggtat aattaataga aggatagaat
      241 aggagcatga tatgaagatt agtgtgaaga aagaggcggg gcactacctg ctcagcattt
      301 gctattctgt gtcggctagt ttgacagaag cttgggactt gttggcgaca gacagtggtt
      361 ttgcccactg gtttccacag ttgaacattg taggaaatac gctggttttt gaaatggaag
      421 gattccgtga agttatggat ttactggaat atcgaaaaaa tgagaaaatt gcctacaggt
      481 gggattcagc aacagtttca tttacactgt ctcagctaga aaatcagacc ttaattactt
      541 ttgaagaacg aattccagaa gattttggta atgaatttgc gaatgctcaa aaagacatga
      601 caggatggtt ggtgccaaat gaatgtatca aaaaattctt agaaggtcag gagccgcctg
      661 tgtgtcaacc cttgcaggaa aagtggagga cttttctgga gcttgaatta gaaggtctgt
      721 gatctgaaag gaggctgtta tgaaaaagtg gtgggaacaa ctgaaagaag aatattatga
      781 aacacggttt gattctaatg aagattggat ggaatggttt atcaaacgga agatgacctg
      841 gggctggaga tggccgactg ctataacgat ttttgtcctt tgggctctat cacttccaga
      901 tgctagaaat gtggttcgtt tcttttattt tttattgacg ctgggagtag ctacggtttt
      961 acataaatta atatcttatt tgaaaagaag aaagtgagag tatatgcatc aaattacttg
     1021 cccccattgt gggacggcct ttcaggtcaa tgaaacagaa tatagtcagc ttttagccca
     1081 ggtgcgtggt gcggagtttg acaaggaaat ccacgaccgt ttggagcggg aacgggaatt
     1141 attaagccag aaggctgaaa atgatttgca ggctagattg agtgataagg ataaggagat
     1201 tctggaactg accgcaaaac ttgataaatt tgctagtcag acggaactgg aaatcagctc
     1261 tgctatttcc caaaaagacc aagaaatcca agagcttaag gctcagttgg ggcaaatcgg
     1321 tcttgccaag gacttggagt tgcagcaggc ggtagctcag gtggaaaaag agcgggatgc
     1381 ggcacaaaat gctttggttt tgcaggaaca aaagcaagag ttagccctag cgaccactcg
     1441 tcaggagtac gaagtacggc tcaaggcggc ggatgagcag gtggaatttt acaaaaattt
     1501 caaggcccag cagtctacta aggcaattgg agagagcttg gagcaatttg ctgaggcgga
     1561 gtttaacaag gttcgttcct atgcttttcc acatgccaaa tttgcgaagg acaacgaagt
     1621 atcagcgtcc ggttcaaaag gagacttcat cttccgtgat tttgacgagt cgggcttgga
     1681 atttatttcc atcatgttcg aaatgaaaaa cgaggcagat acgaccaaga ccaagcataa
     1741 aaacgctgat ttctttaagg agttggataa ggaccgtcgg gagaaaaaat gcgaatatgc
     1801 tgtattagtc agcatgctag aggcggacaa tgactattac aatacaggga ttgtggatgt
     1861 tagtcatgaa tatgagaaga tgtatgttat tcgaccacag ttctttatcc aattaatcgg
     1921 aattttacga aatgctgcgc tcaatagtct gcaatatcag caggagttag ccttggttaa
     1981 ggagcagaat attgatatta ctcattttga agaagatttg gaaattttca agaatgcttt
     2041 tgccaaaaac tatcaatcgg ccagcaacaa tttccaaaaa gctattgatg aaatcgataa
     2101 ggcaatcaag cgaatggagg ctgtcaaggc agccctgacc accagcgaaa accagcttcg
     2161 cctagccaat aataaactag acgatgtctc cgtcaaaaaa ctaacccgca ataacccaac
     2221 catgaaagcc aagtttgagg cgttgaggga ggagtaggat gattgttccg ttacaatttg
     2281 agcatatact tcaacttgat gaggccttcc agaagcaagg ttggccctca agcaaagaga
     2341 ttttaaccaa ctatttaagg gaacaagaaa agggagaacg tatcgtccta gttgctgaag
     2401 aaacgggagt ttgtaaaggt tatatcacct tgattaagca agtgaaggag gggccgtttt
     2461 accaatctgg aatacctgaa attgctgatt tcaatgtttt tgaagacttt caaaatcagg
     2521 ggataggttg gcaattatta gatgctatct gtcagcttgc ctcagaattc acggatatag
     2581 taggtattgg agttggtttg aatgcaaatt atggaaaggc tcaacggctc tatgtaaaac
     2641 atggtttcat accagacgga agcggtgtct ggtaccgagg gcgctctttg ccagtaggtg
     2701 ccaaagccta taacgatgat gaattggcct tgtattttac aaaaaaatta tagaggaaaa
     2761 gtaaaatgaa caccatcgac ctaaatctgc ctgttgcaga gattatcaac cagcacccag
     2821 aagtcaagga tattctggtg gagcttggtt tcaagccttt ggcaaatcca gctatgctca
     2881 acacagttgg taaggtgacc aatctgaaga caggctccaa gatgaccaag attccgttag
     2941 accgtattca acaggtcttg gaatgcaacg gctatgaggt aataggagga gaataatgac
     3001 ggaacaacgg attgacattt tgaagaatat tctcttggac ctgcacaatg gtgccagtcc
     3061 agagtctgtt caggaccagt ttaatcagca tttcacggga gtttctgcct tggaaatctc
     3121 catgatggag catgaactca tgtccagcga cacgggtatc acttttgaag acgtcatgaa
     3181 cctttgcaat atccacgcca accttttcaa aggagccatt gcggatgtgg aagtggcgga
     3241 tatggatcag gaaggacacc ccgtctatgt cttcaagcag gaaaatctag cactgcgggc
     3301 ggctctttta cgcatccgac gtatcattga ccagcatgag cagacagagg atgcggagct
     3361 gaaagaccag ctcctgcaag gtttgacacg ccagtttggc ctcctcggtc aatttgaaaa
     3421 tcactatacc cgcaaggaaa aggtcttctt tccgattatg gagcggtacg gtcatgatgc
     3481 accgcccaag gttatgtggg gtgtggacga cgagattcgc gatctcttca agacggcccg
     3541 caaggcactt gaaggaggtg acctagctgc gacacgtgct cactttgaaa acttcgccaa
     3601 ggaatttgaa gagatgattt tcaaggagga ggccattctc ctcatgattc tgctggagat
     3661 tttcacccag gacgactggc tccaagtggc agcagacagc gatgcctacg gctacgccat
     3721 tgttaagccg acagccaagt gggtacctca tcgggagctt tttggagaaa tagcagagca
     3781 ggaagtagat agcatttctc cgcagccagc tagcccaaac cagcagatta tcgacacgcc
     3841 agaaggccag tttaccatta cctttacacc caaggaaaag tcagaaactg ttcaggacag
     3901 gactagtccg cagaccttcg gcaatggtta tttatctgtt gagcaagcca atcttatcct
     3961 caatcattta ccgctggaat taacctttgt caacaaggac gacattttcc agtactacaa
     4021 tgacagccat cctgttgaag acatgatttt caaacgaaca ccaagccaga ttggacgcca
     4081 tgtggagctc tgccatcctc ccaaaatagt ggacaaggtc aagaagattt ttgaattgct
     4141 ccgcacaggg caaaaagacc aggtcaccat gtggttcaag tccgagagca tgggcaagtt
     4201 tgtctatgtg gtctataagg cagtgcgtga tgaccaagga gagtttcaag gcgttttgga
     4261 gtatgttcag gatattcaac cattttttga gattgatagc gattttcatc gggaaattta
     4321 gtttagtcat ttttgaaagg aaattaagct gttggattgg ccgaccataa aaattggtga
     4381 cgaaggctat tatgatgccc cattgatatt ttcttatggc tatgatgaag aggctgagga
     4441 agggattgat gaagcagtaa cattcgatgc agtaccttct gaaaccatgc gacgcattca
     4501 taaggaacta gagaatttga tagatacaac attcggtagc tatcgtttct atgatgactg
     4561 attgatatcg gaccgcccac aagagcggtc ctttgtgtta taatggaaaa atgatttcag
     4621 gaattattaa cgtaaaaaaa gaggcaggta tgacctcgca tgatgttgtt tttaagctcc
     4681 gcaagatttt gcaggagaag aagattggac atggtgggac cttggatccc gatgtgacag
     4741 gcgttctgcc catcgcagtc ggtaaggcca cgcgtatgat tgagtacatg caggaagagg
     4801 gaaagattta cgagggggag attaccctcg gcttctcaac cacgaccgaa gattcgtcgg
     4861 gcgacctcgt cgagcggaca ccggttttgg acatagcaga gcaggcagtt gatgaggcta
     4921 tgaccagttt cgtcggtact attacccaga ttccgcctat gtactctgct gtcaaagtaa
     4981 acggtcgcaa actatacgaa tacgcacgga cgggtgaaga agttgagcgt cctcaacgcc
     5041 aaattgagat ttacagtttt gaacggacta gcccgattga actagtagat gactgtgcac
     5101 gttttacctt ccgagttcgc tgtggtaagg gcacctatgt ccgtaccctg tctgttgacc
     5161 taggggctaa gttgggctat gccagccata tgtccaagtt ggaacgaaca ggatcagctg
     5221 gaatggacct agcggatgcc ttgactttag aagaaatttc ttcgctggta gctgaaaatg
     5281 atttttcatt cctccaacct attgaacgtg gcatcgggga tttacctgtt gtagaattga
     5341 aagaagagca ggtgcaagat gctacatttg gtcgatttgt agaattgaat gcagaagcag
     5401 agcgcttagc aggctttcat gatggtcagt tgattgctat ttttgaaaaa cgcgaccagc
     5461 tgtataaacc gagcaaggtt ttggtctaag aaatttctca ataactgtga taaaatagaa
     5521 aacatgaaca ttagaacaat taaacattat agtgaattga atcaagagga gccgactgtt
     5581 ttggtattag gctattttga tgggattcat ttggggcata aggccctctt agaccgtgct
     5641 agacaagttg cagatgaaaa aggattgtca gtgaccgtac tgacttttcc agagacccct
     5701 cgtctggctt tttctcgctt tagcccagaa cttttactgc atttgaccag tcaagatcag
     5761 cgttatcact tgttggagca atacggtgta gaccagctgg ttttgacaga ttttaccagt
     5821 gagtttgcaa gcaatactcc tcagcagttt ttagaacgct acatcaaggg gttgaacgca
     5881 caagtcttag tggcaggttt cgattatcat ttcggaaatt gcagagcaga tgtgacagac
     5941 ttgacagagt tatttgatgg tcaggtagaa attgtaagtg aagtaagcct gggcggagag
     6001 aaagtttctt caactcgcat tcgtcaggct atccagtcag gggaggtctc tctagctaat
     6061 caacttttag gttatccatt tatgacggag ggaattgttg ttcatggaga tgccagaggg
     6121 cgcactattg gttatccaac tgcaaatcta gctccttttg accgtgtcca tttaccatct
     6181 gagggagtct acgtcgctga tgtcgaagta gatggaaaac gctatcgagc tatgacaagc
     6241 gttgggaaaa atgtgacctt tgatggaacg gagatgcgga tagaagccca tatttttggg
     6301 tttcaccgct ttatctacgg agaaaaaata actatttttt ggcttgaaaa aattcgagat
     6361 atggtcaaat ttgatggaat tgaaggattg atggagcaga tgaaatctga tgagaaattt
     6421 gccttgcaat gggggaaata tgcttaaacg attgattcgt catccttttt atgagaaggc
     6481 tatgacctac atagctgtag tctatgtatt tcttatcgta cttgaattaa tggatgctag
     6541 tattgccaat tctagtgggt atcgggtact agacttgctc ttatggttta tttttgtttg
     6601 cgattatttt gctcagcttt attatgcgga tgacatactt gaatatgtac agggacatat
     6661 cttggaatta attgccatta ttccatttga ttcattcttc agttttcttc gcttgggaag
     6721 acttgcacga ctcttcagat tggtcaaagt aacgagaata tttgccctgt ctaatcgatt
     6781 ctgggataca atcaacaagc ttttgcatac caacagttta tccaaagtgt tgatgttaaa
     6841 tatctcagca gtgctagttt caagtgtctt attatcagta attgaaggaa aatcattctt
     6901 cgatgcactt tggtggtcca ttgtaacgat gacaactgta gggtatggag atattgtccc
     6961 ccaagatacg atttctaaaa ttatagccat cctcttgatg ttggttggta tctgtacttt
     7021 tggtatggtt acctccacaa taactcgttt tttctctgaa acagagcgag aaacgaagtt
     7081 ggatatcttg atggcaaaaa ttgatgaaca aaacgaaaca ttattacgct tagagaaaaa
     7141 aatagaatct ttagaaaaat taggtgaatt acagggaaga gattagattt actattgaaa
     7201 actcttttaa gttagagtat aataataaga agaaaaatgt ttttagaggg gatggaagac
     7261 atgattactt tatttttgtc accgagttgt accagttgtc gtaaagctcg tgcttggctg
     7321 acaagtcacg aagtcccttt tattgagcac aatattatga ctagccctct aactgctgag
     7381 gaattaaaga acattttagc attgacagaa aatggaacag atgacttgat ttctacccgt
     7441 tcaaaagttt ttcagaaact agatattgat gttgatgagc tttctgtgaa gcagcttatt
     7501 gggttaatcg aaacctatcc gagtctctta cggcgtccga ttattttgga tgagaagcgg
     7561 atgcagattg gttttaacga agatgaaatt cgtgcatttc ttccgcgcaa gtatcgcaaa
     7621 caagagctaa aaaatgctac attgagagca gaaataggat aagaaagata aaagatatga
     7681 acaaaaatta ttcatatcca cttgattttt cgtggagcac agaggagatt tcctccgtgc
     7741 tttctttttt gaatcaggtg gaggcagctt atgaaaaagg agcagatgcg gtagctatat
     7801 tagctagtta taaaagcttc aaagatgttg ttaaaagtaa gggacaggaa cgtcaaattg
     7861 atcgtgactt tgaagcagtt tctggttatt caacctatcg agttgttaaa gctgctcgag
     7921 ataaggggaa aggagttatt cgttttggaa actaagtatc actttgccca aacgattgtc
     7981 ctggaggctg gaaattttct ccgacagcat ttacatgatg atcttcagat tgaagaaaaa
     8041 ggtgacttta cagacttggt aacacacctt gataaacaag tgcaagaaat gttgactcaa
     8101 cagattcaat ctcgctatca aggtgatggg attttaggcg aggaaggtgg ggacgtttct
     8161 tctattcatg agggaaatgt ttgggttatt gatccaattg atggcaccac aaattttatt
     8221 gctcagaaaa ctgattttgc cattatgatt gcctattttg aaaatggagt aggaaagttt
     8281 ggtatcattt atgatgtcat gcgagacaaa cttttccatg gaggagattc attccctgtt
     8341 tattgcaatc aagaacgttt aaagaaacca gagctgcgcc ctcttcgcca gagtttgata
     8401 ggaatcaatg caaagttgta cgcaggcaat gcacatggag tggcagaatt agcaaatcaa
     8461 agtttaggta caagatctgt aggaagcgct gggattggtt tcacccatgt tctggaagga
     8521 agattatttg cctatgcttc ttatctgtgt ccatgggact acgcagcagc cagtattatt
     8581 ggttcctctt taggcttagt catgttaagt tttgagggtc cgagtctgac ctatgataag
     8641 cgagagtttg tcatgctagt atcagaatcc catcttaagg aagtgaaggg gtatatattc
     8701 taatgcaatt accacaggat tttatagata aatataatca tttgcttggt agtgaagccc
     8761 aagcattttt tgagagtttt gaacaagacc caatctcagg atttcgcaca aatcctctta
     8821 aagaaagcca attggacttt gagcaaccaa tccctaatat gacttggtca tactatggta
     8881 aggtctcagg aaagtctcca gaacatgtaa ctggcttagt ctactcacaa gagccagctg
     8941 cacaggttgt aggtcagatt gcagccccgg aaaaagggat gaaagtattg gacttggcag
     9001 cggcgccagg aggaaagtct acccacttac tttcatactt agaaaatacg gggctcttgg
     9061 ttagtaatga aatttctaac aaacgagcaa aaatattggc tgaaaatatt gagcgtttcg
     9121 gggcgagaaa tgtagtcgtg accaatgaat ctgccgatcg tttggccaag gtctttccag
     9181 gatatttcga tatgattgtc ctagacgctc cctgttcagg tgaagggatg tttcgaaaag
     9241 accccgatgc gattcaatat tggtccaaac attatccagc acaatgtgca agcttacagc
     9301 gtgaaatttt agaatcggcc ttgaatatgt tggctccagg tggtcaattg atttattcta
     9361 cttgcacttg gtcaccagag gaaaatgaag aagtagtgac gtggctctta gaacagtatg
     9421 atttggaact aatagacatt cctaaaatca atggaatggt agaaggagtc ggttatccag
     9481 aagttgctcg tatgtatccc caccattttg caggagaagg tcagttcgtt gccaaattta
     9541 gatttctagg agaagtcagt cagaaaaaat taaaaccagg aaaatccaat ttgaatagag
     9601 aacagcaagc tctctggaaa gaatttgaac agaagcattt aagggttcaa ttggatggtt
     9661 tactgcaggt ttttggcgac aatctctacc tactgccaca cggccttcct gatttgtcaa
     9721 aagtaaagat tgctcgaaat ggtcttcact tggggacgta taagaaaaaa agatttgaac
     9781 caagttttgc tttaggtcta gcactccata gttcggaggt tgaaaatcga gtggaaatca
     9841 gcttagaaga attctcgtcc tatactgcag gacataccat agctgtgtcc aaggatttag
     9901 ctaatggctg gtatcaggta gcagtccagg ggaacggtct tggtttcgca aaaattgtat
     9961 ctggaacgct taaaaacggc tttccaaaag ggttgagatt ttaaaataaa tcagcaaatt
    10021 tttttgcaac ttgtcttttt atatgatata gtggatgagt ggaaatatct tgtaaattct
    10081 atgtaaaata cagagttaat tttaggagat tccactgaaa aatgtaaagg aaaaaacgaa
    10141 atgaaaaaaa agcagaagct tggaattgca gctttgtttc ttttgagtag tatggctctt
    10201 tctggttgtt cttcttggat cgatcgagga gagtctatta cagccgtagg ttcgacggcg
    10261 cttcaaccac ttgttgaggc agcagcagat gaatttggta gtgtcaatat cggtaaatcc
    10321 gtcaacgttc agggtggtgg atcaggtact ggcttgtcac aagttcagtc tggagctgtt
    10381 cagattggta actctgacgt atttgctgag gaaaaagatg gtattgatgc aagtaaactt
    10441 gttgaccatc aagtagcagt agctggtttg gccgttattg taaatgagca ggtatcggtt
    10501 aaagatatta ctacagaaga tctgcgtaaa atttttactg gtgagattac caactggaaa
    10561 gaactaggtg gtaaggattt ggaaatttca atcattaacc gtgcagctag ttcaggttca
    10621 cgtgcgacct ttgatagtgt tatcatggat ggacaaaccg caactcagag tcaagaacaa
    10681 gattctaacg gtatggtaaa atctattgtt gctcagacac caggtgcaat ttcctaccta
    10741 tcatttgctt atctagatga ttccgttaaa acaattaagt taaacggttt tgaaccaact
    10801 gctgaaaatg tagcaacaaa tgattggcca atttggtcct atgagcatat gtatacgatg
    10861 ggaaaagcga ctgatttgac agaagaattt ttagattaca tgatgtcaga tgaagttcaa
    10921 aatggtatcg ttgaaagtat gggatacatt tctatcaacg atatgaaagt tgtgaaaagt
    10981 gctgatggca cggtgactga gaaggagtaa gcatgaaaaa cgaacaacta gcaaaagagt
    11041 tatcgtctcc atctaaaaat tctcgattag agaagtttgg taaggtaatt acattccttt
    11101 gtctatcatt gattgtcttt attgttgcaa tgattttgat tttcgttgcg cagcgtggat
    11161 tatcgacttt ctttgttgac ggtgtgagca tcactgattt cttatttgga agcaagtggg
    11221 aaccaagctc gaaaatattt ggtgccctgc caatgatttc aggttctttc attgttacga
    11281 ttctgtcagc tgttgttgcg acccctatcg caatcggtgc agcagtcttt atgacagaaa
    11341 tatcaccaaa acgtggtgca aaaatcttac aacctgttat cgaacttttg gttggtattc
    11401 catcggttgt atatggtttc attggtttgc aagtagttgt accatttgta cgttcaatct
    11461 tcggtggaac aggtttcggt atcttgtcgg gtgtatttgt acttttcgtt atgattttgc
    11521 caacagtaac ctttatgacg gttgatagtt tgcgtgcagt acctcgtcac taccgcgaag
    11581 caagtttggc tatgggagct acccgctggc agacgatttg gcgtgttatt ttgaacgcgg
    11641 ctaagccagg tatttttacg gccgttatct ttggtatggc gcgtgccttt ggtgaagcct
    11701 tggcgattca gatggtagtt ggtaactcag cggttatccc gacttccttg acaacgccag
    11761 cagcgacttt gacttctgtt ttgacaatgg gtatcggtaa tacggttatg ggaactgttc
    11821 agaacaacgt cctatggtcc ttggccctag tcttgctctt gatgagcctt atctttaaca
    11881 tgattatgaa atttattacg agagagggta agaaaaacta tgcacgctaa aaaatttgat
    11941 aaattggcga ctggtattct ttactcaatc gctggtgtga ttgtcgttat cttaacgtca
    12001 ctgattcttt acatcttgct acgtggtctg ccacatgtaa gctgggattt tttgacaagt
    12061 aaatcttcat cttacaaagc tggcggtggt attggaattc agctctacaa ctcattcttc
    12121 ctattggttg taacgctctt aatttctgtt cctttgtcaa ccggtgcagg tatttatttg
    12181 gctgaatatg ctaaaaaagg tccattgact aactttatcc gtacctgtat tgagattttg
    12241 tcatccctac cgtccgttgt cgttggtttg tttggttact tgattttcgt tgttcagttt
    12301 gagtacggtt tttctatctt atcaggtgcc ttggctctta cagtcttcaa cttaccacag
    12361 atgacgcgta acgtagagga cagccttcgt cacgttcacc atacacaacg tgaagcagga
    12421 ttggctcttg gtctatcacg ttgggaaact gttctccatg ttgttattcc agaagcactt
    12481 ccagggattg taacaggtat cgtcttggca tctggtcgta tcttcggtga ggcagcagct
    12541 cttatctaca cggctggtca gtcagctcca gctcttgact ggtcaaactg gaatccattg
    12601 agtgttacta gtccgatttc tatcttccgt caatcagaaa cccttgcggt tcatatctgg
    12661 aaggtaaaca gcgaaggtac aatcccagat gcaacccaag tttctgctgg ttcagcagct
    12721 attctcttgg tcttcatctt gattttcaac ctttctgcac gttatattgg taagaaactt
    12781 catgcgaaac tgacttcagc agcctagaag tctgacttat aaggagaaaa aatggcagat
    12841 tacaactgga atgaacgcca tatcattact ttttcagagg agaatatcgc cctttcaaca
    12901 aaagatgttc atgtttacta tggtaaaaat gaagcgatca agggcattga tatgcagttc
    12961 gaaaaaaata aaattacagc cttgattgga ccatcagggt gtgggaaatc aacctatctt
    13021 cgcagtctta atcgtatgaa tgatactatt gatattgcta aggtaaccgg tcaaattctt
    13081 tatgaaggaa tcgacatcaa tagaccagat atgaacgttt acgaaattcg taagcatatc
    13141 ggaatggtat tccaacgtcc caacccattt gcaaaatcta tttaccgtaa catcacattc
    13201 gcacatgaac gtgcaggcgt gaaagataag caggttttgg atgagattgt tgaaacttct
    13261 ctgaaacaag cagccctttg ggatcaggta aaagatgact tgcacaagtc agcctttacc
    13321 ttatcaggtg gacagcaaca gcgtttgtgt attgcacgag ctatctctgt aaaaccacaa
    13381 atcttgctta tggatgagcc tgcatcagcc ttggacccga ttgcgactat gcagttagaa
    13441 gaaactatgt ttgaattgaa gaaggactac accattatca ttgttacgca caatatgcaa
    13501 caagcagctc gtgcaagtga ttacacagct ttcttctatc tgggtgactt gattgaatat
    13561 gataagacag caaacgtctt ccagaatgca aaactcaagt caactagtga ctacgtttca
    13621 ggacattttg gatagaaagg gaacaaatga catcacctat tttacaagta aaagatttgt
    13681 ctgtttacta taacaagaaa aaagccttga ataatgtgtc aatcgatttc tatccaaatg
    13741 aaattactgc tttgattgga ccatctggtt ctggtaagtc aaccctcttg cgttctatta
    13801 accgtatggg agatttaaac ccagaggtaa cattgacagg tgcgattgat tacaacggaa
    13861 agaacatata cagtcctcgt acggatactg ttgatcttcg taaggaaatc ggtatggtat
    13921 tccaacaacc aaacccattc ccgatgtcta tctacgagaa tgttgtttat ggtttacgta
    13981 ttaacggtat caaggacaag caggtcttgg acgaagcagt tgagcgttcc ttgattggtg
    14041 cttctatttg gaatgaagtg aaagatcgct tgcatgactc agcagttgga ctttcaggtg
    14101 gtcagcagca acgtgtatgt gtggcccgtg tattggcaac aagcccaaaa attattttgt
    14161 tggatgagcc aacgtcagcc cttgacccaa tctcagctgg taaaattgag gacacacttt
    14221 atggtctgaa agataagtat acaatggtgc tagtaacacg ttctatgcaa caggcatcac
    14281 gtatttcaga tcgaacaggt ttcttccttg atggcgattt gatagaatac aatttgacaa
    14341 aagaaatgtt cttgaatcct gccaataaag aaacagaaga ctacattaca ggtaagttcg
    14401 gctaaggcct ataagaaaaa gaaagaggtt cacatgttaa gagctcaatt tgaagaggaa
    14461 ttaggaaaac ttcataacca attttacgcc atgggaaatg aagtattggc tcagattaat
    14521 aatgctgttc gtgcctttac aactcatgat cgtgaattag caaaagaagt cattgaagca
    14581 gataagaaaa ttaatgcttt cgaagtgaaa ttagagaaaa aatcacttga aatcattgct
    14641 ctccaacaac ctgtctcaac agatcttcgt cgcgttatca cagtcttaaa agcaactagt
    14701 gatttggagc gtatgggaga ccatgcagtc tcagttgcta aagcagctat ccgtatgaag
    14761 ggagaagttc gcatcccagt tgttgaagaa gaaatcaaga aaatgggacg tgaagttcgt
    14821 caattagtag aagaaacttt ggaactctac ttaggtgatg ctgatatgga gcgcgcttat
    14881 gaagtagcag cacgtgatga ggttgtcaat gaattctatt caactattca agaattgaca
    14941 acggaagaaa tcaagaagaa tccagaatcc ttggttgctg gtcgcgacta ttaccaagtt
    15001 ttaacctact tggaacgtgt cggtgattat gctaaaaata tttgtgagtg ggttgtttat
    15061 ctacgcacag gtgatattac agagttgtaa ttcagaagtc cgagagggct t
//
LOCUS       DASFSW010000039        15109 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.039, whole genome shotgun sequence.
ACCESSION   DASFSW010000039 DASFSW010000000
VERSION     DASFSW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15109
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.039"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(89..1867)
                     /locus_tag="U1099_000902"
     CDS             complement(89..1867)
                     /locus_tag="U1099_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HEM4867236.1"
                     /translation="MRTAKTILTVIHERGKQDKPLERVYKLLFNRELYLIAYAKLYPN
                     NGAMTKGVTEETIDGMSIQKIDMIIEQLRQETYYWRPARREYIPKKNGKHRPLGIPVW
                     SDKLLQEVIRMILEAYYEPQFSEHSHGFRPKRGCHTALQEIQTWKGTRWFIEGDISSY
                     FDTIDHDVLITMLSRQIQDGRFIRLIKNMLEAGCLDDWKFHKTISGTPQGGVISPLLA
                     NIYLHQFDKWVGEELIPQYTRGKKQKANSAYNRLSRRIKCYQDKGDYKKAHQLIVERR
                     NLPSVDTYDTSYRRLRYVRYADDFILGFTGSKAEAKAIKKQIGDFLNTKLSLELSQEK
                     TLITHATGESAKFLGYEIKAQRVNDYIDNKGRRSANGVIALFVPASVIESKCRQYMKN
                     GKAIHRNNLLHDDDFSIVQTYQQEYRGLVQYYILAQNLSWFSKVYWYMETSLLKTLAF
                     KHKSSINKMLAKYKTTTTSTNGRTVPCLQVVVPREDKPPLVATWGGISLSYKKKAVIE
                     DAPYQVYGGRTELIKRLLANKCELCGSEENIEVHHIRKLADLNKHNGKLVPKWKEIMS
                     ARCRKTLVTCRDCHHAIHNGSINTRL"
     gene            complement(2365..7827)
                     /locus_tag="U1099_000903"
     CDS             complement(2365..7827)
                     /locus_tag="U1099_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM4867237.1"
                     /translation="MNQEVLLQMMRATIPRDRALLEAFLYYQAEHFDEEWDSLIRQFL
                     TNRQGIKRSVQVLHFETDVSAFVQASPYDTAHDLLTYTQVFGQTGLQKLDKLSTSEKD
                     LVIEVALFNLATRFQLLDSNGHYQTISPDSLLQKSRGANLVNVYRVANNLADRISRDI
                     EQFLLTYEPELETGVDETVLENEETVDEHKVSSNQVISFREDGSLIIASLDVDLSQLD
                     VQTGKTSHLPAYEELSLRRKFEILTYFDQIRNERSKVASFRRGDFDTEMEMTPIFEGE
                     ELLTYLEADGSPYELKRTLTRVEEKELEKIGRAIRIENQEKLTQLGIELSQFESDQVG
                     ILLDAAGRFRLKNADLALLGGYPKASVTQLALATELLQMGLSHEMVEFFFGSQLSLEE
                     LRQVAYAFLHEELSREDAEQFEKDKGNQPDLTLRDWKSKLEKAESKVVVDEELAENPL
                     VQRVLDTYPLGSLVSYKGQDFEVMSVSDARLNGLIRIELVNDFSDIVEQNPVLYVRTW
                     EEVSQALHQPKAEPQTELEEADQELNLFSFLEEEPVQSIGLLELDDSENGHNDTDLEE
                     TDNQIPEEVVVETIPAIPVMDFYFPEDMIDFYPKTARDKVETNIAAIRLVKTLETGHR
                     QASKNEQELLAKYVGWGGLANEFFDDYNPKFSKEREELKSLVTDKEYSDMKQSSLTAY
                     YTDPALIRQMWDKLERDGFTGGKILDPSMGTGNFFAAMPKHLREKSELYGVELDTITG
                     AIAKHLHPNSHIEIKGFETVAFNDNSFDLVISNVPFANIRIADNRYDRPYMIHDYFVK
                     KSLDLVHDGGQVAIISSTGTMDKRTENILQDIRETTEFLGGVRLPDSAFKAIAGTSVT
                     TDMLFFQKHLNKEYVADDLAFSGSIRYEMDSRIWLNPYFDGEYNSQVLGTYEVRNFNG
                     GTLSVKGTSDDLIASVETALHHVKAPRKIDRNEVIINPDVLTKQVIDTSIPAEMRENL
                     GQYSFGYQGSTVYYRDNKGIRVGTKTEEISYYVDEEGNFKAWDTKHSQKQIDRFNALE
                     VTDNTALDVYVTDDAAKRGQFKGYYKKTVFYEAPLSDKEVARIKGMVNIRNAYQEVIA
                     IQRYYDYDKETFNHLLGKLNRTYDSFVKHYGYLNSAVNRNLFDSDDKYSLLASLEDER
                     LDPSGKSVIYTKSLAFEKALVRPEKEVKKVHTALDALNSSLADGRGVDFDYMMSIYQV
                     ESKMTLIEELGDLIMPDPEKYLNGELTYVSRQDFLSGDVVTKLEVVDLFVKQDNQDFN
                     WPHYAGLLETVKPARITLADIDYRIGSRWIPLAVYGKFAQETFMGKAYELSDQEVATV
                     LEVSPIDGVITYQSKFAYTYSNATDRSLGVPASRYDSGRKIFENLLNSNQPTITKQVV
                     EGDKKKNVTDVEKTTVLRAKETHLQELFQDYVAGYPEVQQLIEDTYNRLYNRTVSKSY
                     DGSHLTIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELG
                     MVHKPLYVVPSSLTAQFGQEIMKFFPTKKVYVTTKKDFAKAKRKQFVSRIITGDYDAI
                     VIGDSQFEKIPMSREKQVAYINDKLEQLREIKLGSDSDYTVKEAERSIKGLEHQLEEL
                     QKLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVR
                     QVQAEHGDRNVVFATGTPVSNSISELFTMMNYIQPDVLERYLVSNFDSWVGAFGNIEN
                     SMELAPTGDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKIIAVESEL
                     TQAQKYYLEELVKRSDAIKSGSVDPSRDNMLKIVRC"
     gene            complement(7876..8427)
                     /locus_tag="U1099_000904"
     CDS             complement(7876..8427)
                     /locus_tag="U1099_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867238.1"
                     /translation="MAWNFDTIKEALSEMEKVNYQEFIKAFLSLELSISDRTILNQVY
                     QDYMDEDDLSLISDELRVKVDSYQDEVKADMTDILEKLYRTGEGSSFIMDLMSSNSLS
                     DTLEQYEVLDSDDYSPLSLETLQAMIQQDLAISSQDYFGDLVHLALQKDLLDQKSHFL
                     QHYVATVMEGIPQERDQRALVLD"
     gene            complement(8411..8602)
                     /locus_tag="U1099_000905"
     CDS             complement(8411..8602)
                     /locus_tag="U1099_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEM4867239.1"
                     /translation="MNPKSIYEKDSDLDGLTDAQELALGTNPQSVDTDGDGQADLEEL
                     QSGHSPLVPQKELCDGLEL"
     gene            complement(8603..13498)
                     /locus_tag="U1099_000906"
     CDS             complement(8603..13498)
                     /locus_tag="U1099_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4867240.1"
                     /translation="MTKTCNHHFLVNQEKGEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGTVAHADEVTPSVDTTIQRTENPATNLPEAQPTPVSEQTESMASTGQSNGAIAVTV
                     PHDTVTQAVEEAKAEGVSTVEDSQMDLGNTTSAAETNQQISKAEADAQNQVEAINEVT
                     ETYKADKAAYVDEKARIEQENKELSQAYEGANQTGKETNTWVDTKVKDLKTRYADADV
                     TVKEQVVSSGNGTSVLDYTNYGKAVETIQSTNEQAVADYLTKKTKADDIVAKNQAIQK
                     ENEAGLAKAKADNEAIERRNQAGQAAVDAENRAGQAAVDQANQEKQQLVSDRAAEIEA
                     ITKRNQEKEAAARKENEVIDAYNTKEMERYQRDLAEISKGEEGYISEALAQALNLNNG
                     EPQAQHGAITRNPNQIISTGDAMLGGYSRILDSTGFFVYDSFKTGETLSFNYQNLQNA
                     RFDGKKISRVTYDITNLVSPAGTDAVKLVVPNDPTEGFIAYRNDGNGDWRTDKMEFRV
                     VAKYFLEDGTQVTFSKEKPGVFTHSSLNHNDIGLEYVKDTSGKFVAINGSTVQVTNEG
                     LARSLGFNRASDLNLPEEWDTTSSRYAYKGAIVSTVTSGNTYTVTFGQGDMPQNVGLS
                     YWFALNTLPVARTVTPYSPKPHVAVELEPVPEPITVTPDVFTPKTFTPEKPVTFTPKP
                     LEEVVQPSLTLTKVNLPVKPIPKELPTPPQVPTVHYHAYRLTTTPEIMKEVVNSDQAN
                     LHEKTVAKDSTVIYPLTVDALSPNRAQTTSLIFEDYLPAGYLFDKETTQKENGNYVLS
                     FDATKNFVTLTAKENLLQEVNKDLTKVYQLNAPKLYGSVQNDGATYSNSYKLLLNKGT
                     TNAYTVTSNVVTVRTPGDGETTTLITPDKNNENADSVLINDTVVALGTTNHYRLTWDL
                     DQYKGDRSAKETIARGFFFVDDYPEEVLDVVENGTAVTTLDGQKVSGITVKNYASLNE
                     APKDLQDKLARAKITPTGAFQVFLPDDNQAFYDQYVQTGTSLALLTKMTVKDSLYGQT
                     KTYTNKSYQVDFGNGYETKEVTNTLVSPEPKKQNLNKDKVDINGKPMLVGSQNYYTLS
                     WDLDQYRGIKADNSQIAQGFYFVDDCPEEALLPDEAAIQFITSDGKTVSGITVKAYSQ
                     LSEAPKMLQAALSKQKIQPKGAFQVFMPEDPQAFFESYVTKGENITIVTPMTVLETML
                     NSGKSYENVAYQVDFGQAYETNTVTNFVPKVTPHKSNTNQEGILIDGKTVLPNTVNYY
                     KIVLDYSQYKDMVVTDDVLAKGFYMVDDYPEEALTLIPDGIQVLDKDGNRVSGISVST
                     YASLSEAPKVVQDAMAKRQFTPKGAIQVLSSDDPKAYYDTYVKTGQTLVVTLPMTVKN
                     ELTKTGGQYENTAYQIDFGLAYVTETVVNNVPKLDPQKDVVIDLSHKDESLDGKEVAL
                     HQTFNYRLVGAMIPSNRATDLFEYGFEDNYDEKHDEYNGVYRSYLMTDVILKDDSVLK
                     EGTEVTKYTLQQVDTENGLVSISFDKSFLETVSDDSAFQADVYLQMKRIAAGQVENTY
                     LHTVNGYVISSNTVVTHTPQPEEPSPNQPTPPQPPIESLEPPVPASILPNTGEQESLL
                     GLIGAGILLGTAYGLKRKEEK"
     gene            13671..14261
                     /locus_tag="U1099_000907"
     CDS             13671..14261
                     /locus_tag="U1099_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEM4867241.1"
                     /translation="MSKKEILLEFIKNHNGIITYKDCKALKIPTIYLTRLEKEGIIYR
                     VEKGIFLTQNGDYDEYYFFQYRFPKAIFSYISALYLQQFTDEIPQYFDVTVPRGYRFN
                     TPPANLNIHFVSKEYSELGMTTVPTPLGNNVRVYDFERIICDFVIHREKIDSELFVKT
                     LQSYGNYPKKNLAKLYEYATKMNTLEKVKQTLEVLI"
     gene            14258..15103
                     /locus_tag="U1099_000908"
     CDS             14258..15103
                     /locus_tag="U1099_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEM4867242.1"
                     /translation="MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSSYSN
                     HYIFKGGFLLSNVIGVESRSTVDIDFLFHQITLSEETVKQQLKEILADSEEGISFVIQ
                     SITAIKESDDYGGYRATISCQLENIKQVIHLDIATGDVVTPQPITYDYKAIFDEDNFP
                     IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKEDIDFIQLRNACQRTFS
                     YRETELDFVKIIELLERFKSDPIQNKQWQNYSKKYSYTKGISLADVLDEMIRLITVLI
                     SINSD"
BASE COUNT         4172 a         3247 c         2789 g         4901 t
ORIGIN      
        1 aggtagtcat tgcgacccct tttccatcat ccccccaccg aaccggacat gatactttcg
       61 catcatccgg ctctccagta atttcatttc ataaacgtgt atttattgaa ccattatgta
      121 ttgcatggtg acaatcccga caagtcacta gcgtttttcg acatcttgct gacatgattt
      181 ctttccactt tggaactaat ttcccattgt gtttgttcaa gtctgctaat tttctgatat
      241 gatgtacctc tatgttctcc tcacttccac atagctcaca cttatttgcg agtaatcgct
      301 tgataagctc tgttcgacca ccataaactt gatacggagc atcttctatt acagctttct
      361 ttttataaga aagtgaaatg ccaccccacg ttgcaactag aggaggttta tcttctcgag
      421 gcacaactac ctgtaaacac ggcacagttc ggccattcgt gctagttgtg gtagtcttat
      481 atttagctaa cattttatta atggatgact tatgtttaaa tgctaatgtt ttaaggagtg
      541 atgtttccat ataccaatag acttttgaga accatgacaa attttgtgcc aatatataat
      601 attgaaccag ccctctatat tcttgttggt aagtttgaac gatactaaag tcatcatcat
      661 gcaataagtt attacgatga atagctttac catttttcat gtattggcgg catttgcttt
      721 caataaccga tgcaggtaca aataatgcga taacaccatt ggcactccgt ctccctttgt
      781 tatcaatata atcattgaca cgttgtgctt ttatctcata tcccagaaat ttagctgatt
      841 ctccagtcgc atgggtaatc aaagtctttt cttgagaaag ttccaaagat aacttagtat
      901 ttaaaaagtc tccaatttgc ttctttatag cctttgcctc agcttttgaa cctgtaaagc
      961 ctagaataaa gtcatccgca taccgaacat atcttaatct ccgatagctg gtatcataag
     1021 tatcaactga agggagattt cttctctcaa caattaactg atgagctttt ttataatctc
     1081 ctttatcctg atagcattta attcttctac tcaaacgatt ataggctgag tttgctttct
     1141 gctttttacc tcttgtatat tgaggtatca gttcttcacc gacccactta tcaaattgat
     1201 gtaaatagat attagctaat aaaggactaa taactccacc ttgcggtgtt ccactaatag
     1261 tcttgtgaaa cttccaatca tcaagacatc ctgcctcaag catatttttg ataagcctaa
     1321 taaaccgacc atcctgaatt tgtctagaca acatagtgat taatacatcg tggtcaatcg
     1381 tgtcaaaata actagagata tctccttcta tgaaccaacg tgtccctttc caggtctgaa
     1441 tttcttgtaa agctgtatga caccctcttt ttggtctaaa accatgagaa tgttcactaa
     1501 attgtggctc ataataggct tctaatatca tgcgaatcac ttcctgaaga agtttatcac
     1561 tccatactgg tattcccaaa ggacggtgtt ttccattctt tttaggaatg tactctctcc
     1621 tagctggtct ccagtaatag gtttcttgtc ttaattgttc aataatcata tctatttttt
     1681 gaatagacat tccatcaatc gtttcctctg taacaccctt tgtcattgct ccattattcg
     1741 ggtataactt tgcataggct atcagataga gttcacgatt aaatagtaac ttataaaccc
     1801 tttcgagtgg tttatcttgt tttccacgtt catgaatgac cgttaaaata gttttggctg
     1861 tacgcatccc acgcacctaa tcctttctaa ctaatcgaaa ttacctgtca cccttcgcca
     1921 tgtacaagga tttcccttgc tcggactact acggtgactc cgttaccata agggtctccc
     1981 ctcttaggca atcctgctat tcgttaaaaa tgtacgttct agtaaccctt aggttcctca
     2041 ctcattcctt tattcatact tatcgtatgc tcttttactg tagaaagtta ttcgtccaaa
     2101 atcacaacaa cgaatataca gtaacatcgg tctcagttac tttccgatgg gcgatgcttt
     2161 gcacctagtc agaattgagg ttcaagcagt ttagccttaa ccataggtta cggaacttgc
     2221 ggcacatcaa agcgttgtaa ctcgctcctt atccgcttta ctaacatgct attgtcccct
     2281 ttgggtttcc ccgctaggtt agttagttgt tcctattatt tactttcaat aatatccctc
     2341 tgtgagggag atacattttt acgcttaaca gcgcactatc ttaagcatat tatctctact
     2401 tggatcaaca ctacctgact tgatagcgtc tgaacgcttt accagctctt ccaaatagta
     2461 tttctgagct tgcgttaact cgctttccac cgcaataatc ttagcttccg gtactggtaa
     2521 atcaagcatg tctgaggtct gaatatcggc agtttccttg tagattcgca tgagttcagg
     2581 aaggttgaca aatttcttga accgtttctt gggttggtac ttatctcctg tcggggctag
     2641 ttccatggag ttttcgatat tcccaaaagc cccaacccag gagtcaaaat ttgataccag
     2701 gtatcgttcc aagacatcag gttgaatgta attcatcatg gtgaaaagtt cactaataga
     2761 gttagaaact ggtgttcctg tcgcaaaaac gacatttcta tctccatgct ctgcctgtac
     2821 ttgtctcacc ttcatctcca tatccacgtt ctttttagaa gttgtgttgg tgattccagc
     2881 tacattccca agtccagtga ttggacggat attcttgaag tgatgagcct catccacaaa
     2941 aagaaaatca attccaaggt tttcaaattc gataaaggta tctcgctcta gtttttggag
     3001 ttcttccaac tggtgttcca atcccttaat cgaacgttcc gcttctttca ccgtgtaatc
     3061 actgtcactt cctagcttga tttctcggag ttgctcaagt ttgtcattga tataggcgac
     3121 ctgtttttca cgactcatcg gtattttctc aaactgggaa tccccaatga caatggcatc
     3181 atagtcccct gtaataatac gggacacaaa ctgcttgcgt ttggctttgg caaagtcttt
     3241 cttagtagtc acatagactt tcttggttgg gaaaaatttc atgatttctt gaccaaactg
     3301 agcagtcaaa ctagaaggaa ccacatagag aggtttatgc accatcccta attctttcaa
     3361 cttgaaacca gctccaagca tggtcaaagt ttttccagac cctacctcgt gggctagtag
     3421 agcacgtttt tcctcgacaa ttcgttgaat ggcattcttt tggtgaggac gtaaggagat
     3481 attctgagca agtccatcaa tggttaaatg actaccatca taggactttg ataccgtacg
     3541 attgtagagc ctattatagg tgtcttcaat caactgttga acttctgggt accctgccac
     3601 atagtcttga aagagttctt ggaggtgcgt ttccttggca cgcaggactg ttgttttctc
     3661 tacatccgtc acattctttt tcttatcccc ttcgacaact tgttttgtga tggttggttg
     3721 attggaattc aggagatttt caaagatttt tcgaccacta tcatagcgtg aagcagggac
     3781 acctaaactc ctatctgttg cgttggaata ggtgtaggca aacttagatt ggtaagtgat
     3841 aaccccgtca atgggactga cctcaaggac tgtcgctact tcttggtctg acagttcata
     3901 ggctttcccc ataaaggttt cttgggcaaa ttttccataa acagccagag gaatccagcg
     3961 tgaaccgatt cgataatcaa tgtctgctaa agtaatgcgt gctggtttga ctgtttctag
     4021 aagtcctgca taatgcggcc agttaaagtc ctgattgtct tgtttgacaa ataggtccac
     4081 cacttctaac ttggtaacga catcccctga cagaaagtct tggcgagaaa cataggtcaa
     4141 ttctccattc aaatacttct caggatcagg cataatgagg tcgcctaatt cctcaatcaa
     4201 ggtcatcttc gattcaacct gatagataga catcatataa tcgaaatcaa caccacgtcc
     4261 gtcagccaag ctcgaattta aggcatcaag ggcagtatgt acctttttaa cctctttttc
     4321 aggacgcact aaagccttct caaaagcaag ggatttagta tagataacag actttccact
     4381 tggatccaga cgttcatctt ccaaactagc aagaagcgaa tacttatcat cactatcaaa
     4441 aagattgcgg ttcactgcac tgttcaaata cccatagtgt ttgacaaagc tatcataggt
     4501 acgattgagt ttgcctaaca agtggttaaa ggtctcctta tcgtagtcat aatagcgttg
     4561 aatggcaata acttcttggt aggcattgcg aatattgacc attcctttga ttcgtgccac
     4621 ttctttatca gacaatggag cttcatagaa aactgtcttt ttataatatc ccttaaactg
     4681 accacgtttt gctgcatcat cggtcacata gacatccaga gcagtgttat cagtcacttc
     4741 taaggcatta aagcgatcaa tctgcttttg agaatgtttg gtgtcccatg ctttgaagtt
     4801 gccctcttca tcgacatagt aactgatttc ttccgtcttg gttccgactc gaatgccttt
     4861 gttatctcga tagtaaactg tagaaccctg ataaccaaaa ctgtactggc ctagattctc
     4921 cctcatttca gctggaatgg aggtatcaat gacttgtttg gtcaacacat ctgggttaat
     4981 gatgacctca tttctatcaa tctttcttgg ggcttttacg tgatgtagag ctgtttcaac
     5041 acttgcaatc aagtcatcac tagtcccctt aacagaaagt gttccgccat taaaatttct
     5101 gacctcgtag gttcctagca cctggctatt gtattctcca tcaaagtaag gattgagcca
     5161 aatgcgacta tccatttcat aacgaatgga acctgaaaag gctaagtcgt ctgccacata
     5221 ttccttgttc aggtgtttct ggaagaataa catatccgtt gtgacactcg ttcccgcaat
     5281 ggccttaaag gcagaatcag gcagtcgaac cccaccaaga aattcagttg tctcacgaat
     5341 atcttgtaag atgttttctg ttcgcttatc catagttcct gtggaagaga taatcgctac
     5401 ttgtccacca tcatggacca aatcaagtga ctttttgaca aagtagtcat gaatcatgta
     5461 aggcctatcg tacctgttat ccgcaattcg tatattggca aagggaacat ttgaaatcac
     5521 taaatcaaaa ctattgtcgt taaaagccac cgtctcaaag cccttaattt caatgtgact
     5581 atttggatga aggtgtttgg caatagctcc tgtaatagta tctaattcca caccatataa
     5641 ctcactcttt tctcttaagt gtttgggcat tgctgcaaaa aaattccctg ttcccatgga
     5701 aggatctagg attttgccac ctgtaaagcc atctctttcc aacttatccc acatctgacg
     5761 gataagggca gggtctgtat aataggctgt cagagaggac tgtttcatat ccgaatactc
     5821 tttatctgtg actaggcttt tcagttcttc tcgttcctta gaaaattttg gattatagtc
     5881 atcaaaaaat tcattggcta gtccacccca gcctacatac ttggcaagga gttcttgttc
     5941 atttttactt gcctgacggt gtcccgtttc aagtgtttta accaaacgaa tggccgcgat
     6001 gtttgtctca accttatctc tagcagtctt aggataaaag tctatcatat cttctggaaa
     6061 ataaaagtcc attactggaa tcgctggaat tgtttcgact actacctctt caggaatttg
     6121 attatctgtt tcttcaagat cagtatcgtt atgaccattt tctgaatcat ctagttccaa
     6181 tagtccaata ctctgaactg gctcctcttc cagaaatgag aataggttta attcttggtc
     6241 cgcttcttct aactctgttt gtggttctgc ctttggctga tgaagtgcct gactgacttc
     6301 ttcccaggtc ctcacataaa gaactggatt ttgttcaacg atatccgaaa aatcattgac
     6361 taactcaatc cgaatcaaac cgttcaatcg agcatcgctg accgacatga cctcaaagtc
     6421 ctgtccctta taggaaacca atgaccccag aggataagtg tccaatactc tctgaaccag
     6481 tggattttcc gctaactctt catcaaccac tactttcgac tcagctttct ctagcttgct
     6541 tttccaatct ctgagggtta aatctggctg attaccttta tctttttcaa attgctccgc
     6601 atcttctctg ctgagttctt cgtgtaaaaa ggcgtaggca acttgtcgca gctcttcaag
     6661 ggaaagctgg ctaccaaaga aaaattcaac catttcatga cttagtccca tttggagtag
     6721 ttctgtcgca agggctagtt gagttaccga ggctttggga taaccaccta ataaagcaag
     6781 gtccgcattt tttaaacgaa aacgacctgc cgcatccaat aaaataccga cttggtctga
     6841 ttcaaactga gatagctcaa tccctagctg agtcaatttt tcttgatttt ctatcctaat
     6901 agctcgtcca attttttcta attccttttc ttcgactcta gtcagcgttc gtttcagctc
     6961 atagggacta ccatcagcct ctagataggt aagtaactcc tcgccctcaa agattggtgt
     7021 catttccatc tcagtatcaa aatcacctcg tctaaaactt gcgactttgg aacgttcatt
     7081 tcgaatttgg tcaaaatatg ttagaatctc aaatttacgt cgtaaagata actcttcata
     7141 agctggcaga tgactggttt ttcctgtttg aacatctagt tgagacaaat ctacatccaa
     7201 actagcaatg atgagagagc catcttctct aaatgatatt acttgattac tactcacttt
     7261 gtgctcatca acagtttctt cattttctag aactgtttca tcaactcctg tttcaagctc
     7321 aggctcgtaa gtcaatagaa actgctcaat atctcggctg attcgatccg ctaaattatt
     7381 agccacacga taaacattga ccaaattagc tcccctactc ttttgtaata gagaatctgg
     7441 tgaaatagtt tggtagtgtc catttgagtc taatagttga aaacgagtgg ccagattgaa
     7501 caaggccact tcaatcacca agtctttttc agacgtcgat agtttgtcaa gtttttggag
     7561 accagtttgg ccgaatactt gtgtataggt caatagatca tgagcagtat cataaggact
     7621 agcctgaaca aaagctgaaa catctgtctc aaagtgaagt acctgaacag atcttttgat
     7681 tccctgccta ttggtcaaaa actgacgaat aagactatcc cactcctcat caaaatgctc
     7741 tgcttggtaa tataaaaacg cttcaagcaa ggctctatca cgaggaatgg tggctctcat
     7801 catttgtagt aagacttctt gattcatttt ccctcccatt ttaaaagggc tgagagtttt
     7861 tctcagccct ttgcgttaat ctaggaccaa ggctcgttgg tctctttctt gtggaatacc
     7921 ttccattaca gttgccacat agtgttgtaa gaaatgactt ttctggtcta gtaaatcttt
     7981 ttgcaaggca aggtgaacca aatctccaaa ataatcctga ctggaaatag ccaagtcctg
     8041 ttgaatcatg gcctgtaagg tttcaaggct aagtggtgaa taatcgtcac tatccaaaac
     8101 ctcatactgt tccaaggtgt ctgagagact attggaggac attaaatcca taataaaact
     8161 agagccttca cctgttcggt acagcttttc cagaatatca gtcatatcag cttttacttc
     8221 atcctgataa ctatcaactt taactcgtag ctcatcacta atcaaggata ggtcatcctc
     8281 atccatataa tcttgataaa cctgattgag gattgttcta tcagaaatac ttaattccaa
     8341 agagagaaag gctttgataa actcttggta attaaccttc tccatttcag acaaagcctc
     8401 ttttatggtg tcaaagttcc aagccatcac acaactcctt ttgtgggact agtggtgaat
     8461 gtccagattg tagctcttct aaatcagctt gaccatcacc atctgtgtca acagactgcg
     8521 gattggttcc caaagctagt tcctgagcat ctgtcagacc atccaggtct gaatcctttt
     8581 cataaatgct ttttggattc atctacttct cctcctttct cttcagtcca taagccgtac
     8641 caagtagaat accagctcca attaagccca aaagggattc ctgttcccct gtatttggca
     8701 aaatgcttgc tggaacaggt ggttcaagag actcaatcgg tggttgaggt ggagtgggtt
     8761 gatttggact tggttcttca ggttgaggtg tatgtgttac aactgtattt gaactgatga
     8821 catagccatt cactgtatgg agataggtat tctccacctg accagccgca atccgtttca
     8881 tttgaaggta aacatctgcc tgaaaggctg aatcatcaga gacagtctct aagaaggatt
     8941 tatcaaatga aattgacact aggccatttt ctgtatccac ttgttgcaag gtatatttcg
     9001 taacctccgt tccttctttt aagacagaat cgtctttgag gatgacatcc gtcatcagat
     9061 agctgcgata aacaccatta tactcatcat gcttttcatc atagttatct tcaaaaccat
     9121 attcaaataa atcagtcgca cgattgcttg gaatcatcgc tccaaccaag cgatagttaa
     9181 aggtttgatg caaggccact tctttcccgt caaggctctc atccttatga gacaagtcaa
     9241 tcaccacatc tttttgtggg tctagtttgg gaacattatt gaccactgtt tccgtgacat
     9301 aggccaagcc aaaatcaatc tgataggctg tattttcata ctgaccacct gttttggtca
     9361 actcattttt taccgtcatc ggaagcgtga caactaaggt ttgaccagtc ttcacatagg
     9421 tatcataata ggcttttgga tcatcgctac taagaacttg aatggctcct ttaggtgtaa
     9481 actgacgttt agccatggca tcttggacga ctttcggagc ttctgacaaa ctagcgtagg
     9541 tgctgacgga gataccagat acacgattgc catccttatc caaaacttga atgccatcag
     9601 gaattagggt aagggcttct tctgggtaat cgtctaccat gtaaaatccc ttggcaagaa
     9661 catcatccgt cacgaccatg tccttgtatt gactgtaatc caagacaatt ttgtaataat
     9721 tgaccgtatt cggaaggaca gtctttccat caattaaaat gccttcttga ttggtattag
     9781 acttatgtgg agttacttta gggacgaaat tagtcaccgt attggtttca taagcttggc
     9841 caaaatccac ctgataggcc acgttttcat aagacttccc tgaattaagc atggtttcca
     9901 aaaccgtcat cggagtgaca atggtaatat tctccccctt ggtcacataa gattcaaaaa
     9961 aggcttgtgg gtcctcaggc atgaaaactt gaaaagctcc tttaggctga attttttgtt
    10021 tcgaaagggc tgcttgtagc attttaggag cttctgataa ttgagaataa gccttcaccg
    10081 tgattcctga aactgttttg ccatcagatg tgataaactg aatagctgct tcatccggca
    10141 ataaagcttc ttctggacaa tcatccacaa agtaaaaacc ttgtgcaatc tgagagttgt
    10201 ctgctttaat ccctcggtat tggtccaagt cccatgagag agtatagtaa ttttgacttc
    10261 ccactagcat gggcttccca ttgatatcca ctttgtcttt attgaggttc tgtttcttgg
    10321 gttctggaga aacaagggtg ttggtcactt ccttggtttc atagccattt ccaaaatcaa
    10381 cttgataaga cttgtttgta taggtcttag tttgaccata gagactatct ttcaccgtca
    10441 ttttggtcaa aagagctaaa gaagttcctg tttgaacata ctggtcataa aaggcttggt
    10501 tgtcatccgg caaaaagact tgaaaggcac ctgtcggtgt aatcttagca cgagccaatt
    10561 tatcttgaag gtctttaggg gcttcattta gggaagcata gtttttaacc gtaattcctg
    10621 ataccttctg accgtcaagg gttgtaacag ccgtcccatt ttccaccaca tcaagcactt
    10681 cctctgggta atcgtccaca aagaagaagc ctcgtgcaat tgtctcttta gcagaacgat
    10741 ctcccttata ctggtccaaa tcccaagtca aacgatagtg gttggttgtg ccaagggcta
    10801 cgaccgtgtc attaatgagg acactatccg cattttcatt gtttttatct ggtgtaatga
    10861 gtgtggttgt ctccccatca cctggtgtac gaaccgttac aacatttgaa gtgactgtgt
    10921 aagcatttgt tgtgcccttg ttcaaaagga gtttgtaact attggaatag gttgccccat
    10981 cattttgaac agaaccatag agttttgggg cgttcagttg ataaaccttg gttaaatctt
    11041 tatttacctc ctgcaacaag ttttcctttg cggttaaggt cacaaaattc ttagtcgcat
    11101 caaagctaag gacatagttt ccattctctt tttgtgttgt ttccttatca aataggtaac
    11161 cagcaggcaa gtagtcctca aaaatgagac tagtcgtttg ggcacgattg ggcgataagg
    11221 catcaactgt taagggataa atcaccgttg aatcttttgc gacagttttc tcatgaagat
    11281 tagcttggtc actattgact acttctttca taatctctgg agttgtcgtc aaacggtacg
    11341 catgataatg gacagtcgga acttgtggtg gcgttggaag ttcttttgga ataggtttaa
    11401 caggtaagtt taccttggtc aaagttagac taggctgcac tacttcttcc aaaggctttg
    11461 gcgtgaaggt cacaggcttt tcaggtgtaa aggtttttgg agtaaagaca tctggtgtta
    11521 ccgtaatagg ttctggaacg ggttcaagtt ccaccgctac atgaggtttg ggactataag
    11581 gtgtcactgt acgtgcaact ggtagggtat ttaaggcaaa ccagtaagac aatccaacat
    11641 tctgtggcat atcgccttgg ccaaaggtta ctgtgtaggt attgcctgat gtaactgttg
    11701 agacaattgc tcctttataa gcataacgac ttgatgtggt atcccattcc tcaggcagat
    11761 tcaaatcact tgcacggttg aaacccaaag aacgggctag accttcatta gtcacttgaa
    11821 cggttgaacc attaatcgcc acaaatttcc cagaagtatc tttgacatat tctagaccaa
    11881 tgtcattatg attgagggaa gaatgcgtga agactcctgg tttttctttg gagaaggtaa
    11941 cctgcgttcc gtcttccaag aaatacttgg caactacacg aaattccatc ttgtctgttc
    12001 gccaatcacc atttccatca tttcgatagg caataaaacc ttcggttgga tcatttggca
    12061 caaccaattt cacggcatcg gttccagctg gtgatacaag gttggtaata tcgtaagtca
    12121 ctcgactaat ctttttacca tcaaatcgtg cattttgaag attttgatag ttaaaactaa
    12181 gggtctcacc tgttttgaag ctatcataga caaagaatcc tgttgaatcc aatattcttg
    12241 agtagccacc cagcatggca tcgccagttg aaatgatttg attaggattt cgggtaattg
    12301 ctccatgttg tgcttggggt tcaccgttat tgagattgag ggcttgagca agggcttcag
    12361 aaatataacc ttcttcacct ttcgaaatct cggccaagtc acgttgatag cgttccattt
    12421 ctttggtatt gtaggcatca atcacttcat tttcttttct ggctgcggct tctttttctt
    12481 gattacgttt tgtgatagct tcaatctcgg ctgcacgatc tgaaactaat tgttgtttct
    12541 cctgattagc ttgatctact gcggcttgac ctgcacggtt ttcagcatca acagctgctt
    12601 gacctgcctg attacgcctt tcaatggctt cattatctgc ctttgcctta gcaagtccag
    12661 cttcattttc tttttgaatg gcctgatttt tcgcaacaat atcatctgcc ttggttttct
    12721 ttgttagata atccgctaca gcttgttcgt tagttgattg aatagtttca acagccttgc
    12781 catagtttgt atagtccagt acagatgttc cgtttcctga tgagactact tgttctttta
    12841 ctgtcacatc agcatctgca taccgagttt ttaggtcttt gactttggta tcaacccaag
    12901 tatttgtctc tttaccagtt tggttggccc cttcgtaggc ctgtgacagc tccttatttt
    12961 cctgttcgat gcgagctttt tcatccacat aagctgcttt gtcagctttg taggtttcag
    13021 taacttcgtt gatagcctca acttgatttt gggcatctgc ttctgctttt gaaatttgtt
    13081 ggttagtttc tgccgcagaa gttgtatttc ctaaatccat ttggctatct tcaaccgtag
    13141 aaacaccttc agcctttgcc tcttcaactg cttgtgttac cgtatcatgt ggtacggtaa
    13201 cagcaattgc accgttagat tgtccagttg atgccatact ttcagtttgt tcagaaacag
    13261 gggttggttg tgcttctggt aaattcgtgg ctggattctc cgttcgttgg atggtggtgt
    13321 caactgatgg agtaacttca tcagcatgtg caaccgttcc tccccaagca acaacagctg
    13381 tcaccatggt tccaagggca acggaacata aagtacgata ttttttactc ttgcgaaaga
    13441 cgtgtttctc gcctttttcc tgattgacaa gaaagtgatg attacatgtt ttggtcataa
    13501 atagtccttc ttttctttta ttctttagca acaaactgtc actctttcct catcataaaa
    13561 aagaaggctt gattttgtta tcacaataag ttcagggaaa agaaaatgtt gcttttacac
    13621 tacaaatatt gtaaaattgt agtgtgaaag caacacaaaa aggagtaagc atgtcaaaaa
    13681 aagagattct acttgaattt ataaaaaacc acaatggcat tatcacctat aaagattgta
    13741 aagctctaaa aattccaaca atatatttaa caagactaga aaaagaaggg attatatacc
    13801 gcgtcgaaaa aggaattttc ctaacccaaa acggagacta tgatgaatac tacttctttc
    13861 aatatcgttt tcctaaggct attttctctt atatttcggc actgtatcta caacagttta
    13921 cagatgaaat tccacaatat tttgatgtga cagttcctag aggctaccgc tttaatactc
    13981 ctccagctaa tctaaatatt cactttgtct ctaaagaata cagcgagctg ggaatgacta
    14041 ctgtacctac tcctttggga aacaacgtaa gggtatatga ttttgaacgt attatttgtg
    14101 attttgtaat acatcgagaa aagattgatt ccgaactctt tgtcaagaca cttcaatctt
    14161 atgggaacta tcctaaaaaa aatctagcaa aactctatga atatgcgaca aaaatgaata
    14221 ctttagaaaa agttaaacaa actctggagg ttctaatatg aacaaagcta aattaacagc
    14281 actctgtcat aaaatatcaa agaatactgg tttaaccttt aattccgtca tgacctatta
    14341 cttcctagaa gtcattttga aaaaattaag tcaaagttct tattcaaatc actatatctt
    14401 caaaggagga ttcctacttt cgaatgtcat cggagttgaa tctcgtagca ctgttgatat
    14461 tgacttttta ttccatcaaa taacactctc agaagaaact gttaaacagc aactcaagga
    14521 aattttagca gattccgaag aaggtatatc ttttgtgatt caatcaatca cagctattaa
    14581 agaaagtgat gactatggtg gctatcgagc aacaatttcg tgccaacttg agaatattaa
    14641 acaagttatc catttggata ttgcgacagg tgatgttgta actcctcagc cgattacata
    14701 cgactataaa gctatttttg atgaagacaa ttttccaatc attgcttata caattgaaac
    14761 aattctagct gaaaaacttc aaaccatcta ttcacgtaac ttcttgaaca gtagaagcaa
    14821 agatttttac gatgtttata ttctttccaa actgaagaaa gaagacattg acttcattca
    14881 gttgagaaat gcctgtcaga gaacattttc atatcgcgaa acagaactcg attttgtaaa
    14941 gattattgaa ctactcgaga ggttcaaatc tgaccctatt cagaataaac aatggcaaaa
    15001 ttattcaaaa aaatatagct atacaaaagg gatttcactt gcagatgttc ttgatgagat
    15061 gattcgtctc ataacagttc tcatttctat aaattctgat taatcgtga
//
LOCUS       DASFSW010000040        14877 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.040, whole genome shotgun sequence.
ACCESSION   DASFSW010000040 DASFSW010000000
VERSION     DASFSW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.040"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(144..932)
                     /gene="yghU"
                     /locus_tag="U1099_000909"
     CDS             complement(144..932)
                     /gene="yghU"
                     /locus_tag="U1099_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEM4867243.1"
                     /translation="MTEYTKPLVWKWEDENDNRSGNRPTAGSRFEQALPKGEKSFQVY
                     SLGTPNGIKVAIMLEELKELGISEADYDLFLINIGQGDQFGSDFVAINPNSKIPAMVD
                     YSEQEPISVFESANILLCLAEKFDAFLPKTWAERTEVLNWLFWQTGAAPFVGGGFGHF
                     FHYAPTAQEYPINRYTMEAKRQLDLLDQLLATREYIAGNTYTIADIAIWSWYGRLVQG
                     QLYPGSAEFLDVDSYKHLKTWVEKIAQRPAVQRGLTVKYHSIDN"
     gene            complement(941..1231)
                     /locus_tag="U1099_000910"
     CDS             complement(941..1231)
                     /locus_tag="U1099_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867244.1"
                     /translation="MKVAEKEELYKYLSAAYNLPQEAFSEALREKILEVAGQLDKEEN
                     LYILAGHLSRFINAELTALTCRAPKELVQLAHYLQEVQNHYRYASLFPGKVK"
     gene            complement(1337..2026)
                     /gene="rplA"
                     /locus_tag="U1099_000911"
     CDS             complement(1337..2026)
                     /gene="rplA"
                     /locus_tag="U1099_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEM4867245.1"
                     /translation="MAKKSKNLRAALEKIDSTKLYSVEEAVALAKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAVVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGIVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMTSVSITTTQ
                     GVGIKVDPNSL"
     gene            complement(2067..2492)
                     /gene="rplK"
                     /locus_tag="U1099_000912"
     CDS             complement(2067..2492)
                     /gene="rplK"
                     /locus_tag="U1099_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEM4867246.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAASLEAAMRMIEGTARSMGFTVTD"
     gene            2647..3015
                     /locus_tag="U1099_000913"
     CDS             2647..3015
                     /locus_tag="U1099_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEM4867247.1"
                     /translation="MFESIIFLKIVAFLFIFLTLAISIIAVKFFRLQNRGWNFADIAF
                     PLYAIEFYLISDKAYYNSLLPQLVLALSLLAIGLCGFFLLKKKNFLYRRFFKVFWRAS
                     FILTFLMYLALVIAVFTLKS"
     gene            complement(3378..3764)
                     /locus_tag="U1099_000914"
     CDS             complement(3378..3764)
                     /locus_tag="U1099_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM4867248.1"
                     /translation="MKFDAVHHIAIIGSDYDKTREFYVEKLGFEQVDEHIRPEKNDIL
                     FNVKKGNLILEIFIKPDAPMRPAMPNPEHTGLRHLAFQVADVEACLEEFDRLDIRHEV
                     LRTDDFDGKKMAFFFDPDGLPLEIHE"
     gene            complement(3764..6133)
                     /locus_tag="U1099_000915"
     CDS             complement(3764..6133)
                     /locus_tag="U1099_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEM4867249.1"
                     /translation="MRKTSKKGKATRRPTKAELAQQERVKNITLRVLGLLFIAFAASR
                     LGVFGVTSYNIFRLLFGSLAYLLLAGAFIYLLIPKVLREREGTISGFWLIVIGLLIEF
                     QAYLDWTYQGSDLFGHTLKLALSDLAKFQVTAFLGGGMIGSIFYLPVSFLFANVGSFF
                     IGLLVIAFGIFFVSPWSVYDVADGLAVAKDKLVETQVKRAELRTQKKAEREEKRQQEL
                     ARLEEERLRLEEEEESARALALVDRHVDLETGEILEEEPVQVPIISEYDHLDIEDEFP
                     IMLAEDDQPAQSNSASQTSEPNEFEKDEIDVQIDFKPKQRLAYKLPSIDLFAPIKAKS
                     QSNEKRIVRQNIKVLEDTFASFGIKVVVERAEIGPSVTKYEVKPAVGVRVNRISNLAD
                     DLALALAAKDVRIEAPIPGKSLVGIEVPNSEVATVPFRELWEQSKTDPDKLLEIPLGK
                     AVNGSVRSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKAGPDQVKFMMIDPKMVE
                     LSVYNDIPHLLIPVVTNPRKAARALQKVVDEMEKRYELFSQIGVRNLEGYNAKVEEFN
                     SRSEEKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIHMILATQRPSV
                     DVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPIDENHPVRLQG
                     SFISDDDVEAIVGFIKDQADADYDESFDPGEVAEGDGDTGFGDTGGDPLFNEARALVV
                     ETQKASASMIQRRLSVGFNRATRLMEELEAAGVIGPAEGTKPRKVLETQ"
     gene            complement(6259..7617)
                     /locus_tag="U1099_000916"
     CDS             complement(6259..7617)
                     /locus_tag="U1099_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEM4867250.1"
                     /translation="MKTVVEANQFIQTEKGNVNPIFKPQAHLTPETGWINDPNGFIYF
                     RGEYHLFYQFNPYESVWGPMHWGHAKSKDLVNWEQLPVALAPDKAYDKDGCFSGSAIV
                     KDDVLWLMYTGHINNEDGTVSQVQNMAFSTDGIHFEKIEQNPVATADGLPEEVIANDF
                     RDPKIFEKDGHYYSVVATKHKDNVGCIVLLSSPNLTDWKFESIFLKGEANQGFVWECP
                     DYFEVDGQEYLIISPMRYQKDGNDFININSNIFVMGHVDWDKKVFVAESFKEIDHGHD
                     FYAAQTTEGPEGERVMIAWMHTWGRPLVTNDLDHKWYGQMTLPRILKKTENGLHQVLP
                     TSVFEAFKDIEIGQSIQGPSKLSLRLEDSLELKLGNDQDYLQFGYDKAKQEVYINRSS
                     LKIQQVGEEEWSTVRRAVTVQATELEVLVDTNCLEIFINDGQETLTSTFYVEGQIILN
                     AL"
     gene            complement(7635..9245)
                     /locus_tag="U1099_000917"
     CDS             complement(7635..9245)
                     /locus_tag="U1099_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4867251.1"
                     /translation="MKHKFGKTVVTLLASTVLLAACGSKNTASSPDYELKDVQFPLEK
                     SVTLKFMTASSPLAPADPNDKLIFQRLEEETGVHIDWTNYQSDFGEKRNLDIASGDLP
                     DAIHNDGASDVELMNWAKQGVIVPVEDLIDKHMPNLKKILDENPEYRSMITAPDGHIY
                     SFPWIEELGEGKESIHTVNDIAWINKEWLNKLGLEMPKTTDDLIKVLEAFKTQDPNGN
                     GKADEIPMSFVNGNGGEDFKILFGAFGVGDNDDHIVVNNDGTVDFTADNEDYKEGVEF
                     MRTLQEKGLIDPEAFEQDWNTYLAKGSENLYGVYFTWDKANITGMNETYDILPVLAGP
                     DGTKHITRTNGMGFQRDRMVITSANKNLELTAKWIDAQYKPIQSVQNNWGTYGDETQQ
                     NIFEYDAATDSLKHLPLNGTAPGELRQKTEVGGPLAILDEYYGTVTTMPDDAKWRLDL
                     MHATYLDYVTNNDIYPRVFMEQEDLDKIAQVEADMNDFIYRKRAEWIVNGGIEEEWDS
                     YLKELESYGLSDWLAIKQKYYDQYKENQ"
     gene            complement(9445..10332)
                     /locus_tag="U1099_000918"
     CDS             complement(9445..10332)
                     /locus_tag="U1099_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM4867252.1"
                     /translation="MNTQMYTKFDKRMLIVNKILLAFLVLITVVPMVYILVASFMDPQ
                     ALVSKGISFNPKDWTVEGYQRVFSDKSILRGFFNSLFYSFSFAFVTVAISVMTAYPLS
                     KKDLVGKKWINAFLVFTMFFGGGLVPTYLLVKDLGMLNTVWAIIIPGAVNVWNIILAR
                     TYFQGLPDELVEAAVMDGANEFQIFWKIMIPLAKPIMFVLFLYAFVGQWNSYFDAMIY
                     IKDPDLEPLQLVLRKILIQSEPAKDMIGAQAAMNEMKRIAEMIKYATIVISSLPLIVM
                     YPFFQKYFDKGIMAGSLKG"
     gene            complement(10342..11262)
                     /locus_tag="U1099_000919"
     CDS             complement(10342..11262)
                     /locus_tag="U1099_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM4867253.1"
                     /translation="MKTSKLQQAGLVDRIKQQKLLLLMLLPGLVLTFIFRYIPMYGVL
                     IAFKDYNPLKGVLGSEWIGFEEFTKFLSSPNFGTLLANTLKLSVYGLLLGFLPPIILA
                     IMLNQLLSDKAKKRIQLVLYAPNFISVVVIVGMIFLFFSVGGPVNSILGMFGIEANFL
                     TDPDFFRPLYILSGIWQVMGWASTLYTATLVNVDPALIEAAKLDGANIFQRIWHIDLP
                     ALKPVMVIQFILAAGGIMNVGYEKAFLMQTSLNLTSSEIISTYVYKIGLVSGDYSYST
                     AVGLFNALINIILLIAVNKIVKRINDGQGL"
     gene            complement(11417..12406)
                     /locus_tag="U1099_000920"
     CDS             complement(11417..12406)
                     /locus_tag="U1099_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM4867254.1"
                     /translation="MEEQKSITMKDVARVAGVSVGTVSRVINNEPGIKESTLEKVNTA
                     IRELNYIPDVYARGMKKNKTETIALIIPTVWHPFFGEFAYHVEVELSKKNYKLLLCNI
                     SGPKRELDYLTMLQQNKVDGIIAITYSPIEDYLSSNIPFVSIDRVYENKDIACVSSDN
                     RAGAELAANTLIAKGGRHFAFIGGHNKTINETKKRRLYFEKRILEAGFPCHVLDLEEP
                     YDDFVGQVEEFLINNPQVDAIFTINDFTALDTLAVLEKLGRRVPEDVQVIGYDGIQLA
                     SEKSLALSTIRQPLESMAQEAVACLIDIIEKRERPLQVTLPISYLEGKTTKKF"
     gene            complement(12563..13108)
                     /locus_tag="U1099_000921"
     CDS             complement(12563..13108)
                     /locus_tag="U1099_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867255.1"
                     /translation="MSLQNRVEEMYKDHEVKPYISPERDLAAWLLEAKPVPKRNMVRL
                     EEGILPGDIILLWRISLGSFETTTPYSKYFEYMYGINGPAHMEQLIADGYAYVESAFD
                     SLDHITSTAKKNILKAEGVTGLSKMKAADLDTALKDNLTEEKLAPYFTVRGYALTEKG
                     RAALENHPEVLAKHPMKKMYK"
     gene            complement(13109..13486)
                     /locus_tag="U1099_000922"
     CDS             complement(13109..13486)
                     /locus_tag="U1099_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HEM4867256.1"
                     /translation="MIQSIGQVMLYVNDIEASAQFWKEKMGFERVEKQVQGPQTSYII
                     APKSDSEVQFVLHDKAEVAEMEPELNLGTPSILMTSVDVAKTYEELVERGVTTNPVMD
                     LGFMKVISFADNDGNYYAIREVK"
     gene            complement(13571..13924)
                     /locus_tag="U1099_000923"
     CDS             complement(13571..13924)
                     /locus_tag="U1099_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HEM4867257.1"
                     /translation="MFENLFAPDGSLVWHTALFYSFSAIILATVCFHIIKNFMEWHHN
                     NRAPKESCKAKLVSKRTHVFGNEVARTNYYVTFEWEGKRQEFRLRSDEYALLAEGDVG
                     TLHFQGTRFLGFERL"
     gene            14067..14870
                     /locus_tag="U1099_000924"
     CDS             14067..14870
                     /locus_tag="U1099_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM4867258.1"
                     /translation="MKKILVLALASSILLTACTNNSTANSSSSTASSSSPSSTTTADT
                     TASSQYAKDLAYAINNPKATFPQLSNEIGENEAAVKIKTTEGDITIKLFPEQAPLTVE
                     NFLTHAKEGYYNGTIFHRVIKDFMIQGGDPLGNGTGGESIWSGKDTTIDAGNGFKDEI
                     SAFLYNIRSSLSMANAGAGTNGSQFFINQNTTDMSSQLSSSSYPGKIIDAYKNGGNPN
                     LDGKHTVFGQVIEGMDIVDKIASVETDNSDKPKTDVKIESIEILKDYTF"
BASE COUNT         4178 a         3219 c         2963 g         4517 t
ORIGIN      
        1 cgtcgcttag tctaattctg attttcattg agtatcaatt attaatttct atttttggat
       61 tttcacaaag tctaactctt tcattataaa cttttatcat atcaaagtaa aagaaaaacc
      121 cacccataag gtgggcaagt caattaatta tcaattgaat gatatttcac tgtcaaccct
      181 cgttgtacag ctgggcgctg agctattttc tctacccagg tctttagatg cttgtaagaa
      241 tcaacatcta aaaattctgc tgaacctgga tatagttgac cttgcaccaa gcgaccatac
      301 caagaccaaa tagcaatatc tgcgatggta taggtatttc ctgctatata ttctcttgtc
      361 gccaatagct gatccaagag atctagctga cgcttagctt ccattgtata gcgattaatc
      421 ggatattctt gcgctgtcgg agcgtaatgg aagaaatgtc caaatcctcc accaacaaat
      481 ggagcggcgc cagtttgcca gaagagccaa ttaagaacct ctgtccgctc tgcccaagtt
      541 tttggtaaaa aggcgtcaaa cttttctgcc aggcaaagta gaatattggc tgattcaaag
      601 accgaaatcg gttcttgctc ggaataatct accatagctg gtattttgga gttggggttg
      661 atggcaacaa aatcggagcc aaattggtct ccttgaccga tattgatgag aaagaggtca
      721 taatcagctt ccgaaatacc taactctttc aactcctcca acataatagc aaccttaatg
      781 ccattcggcg tccccaatga atagacttgg aaagacttct ctccttttgg taaagcttgc
      841 tcaaaacgac tacctgctgt tggacggttg ccagatcggt tgtcattttc atcttcccac
      901 ttccaaacga gtggttttgt atattctgtc atgttttctc ctattttacc tttcctggaa
      961 aaagactggc atagcgataa tggttttgaa cctcttgaag gtaatgggct agttgcacca
     1021 attccttagg cgctcgacaa gtcagagctg ttaactctgc attgataaag cgacttaagt
     1081 gtcctgccag tatatagaga ttttcctcct tgtccagctg acctgccacc tccaatattt
     1141 tttcacgcag ggcttctgaa aatgcttcct gcggaaggtt gtaggctgca gaaaggtatt
     1201 tataaagctc ttctttctct gctactttca tcggataact ccttttatgt aaattgaata
     1261 tagtataaat cgaagtcaag tgcttgtcaa gaaaatctca aaaaaaaaga ttgccctaag
     1321 acaatctttc tattgattaa agtgagttag gatcaacttt gataccaaca ccttgtgttg
     1381 ttgtaataga tactgaagtc atgtaagtac ctttagctgt agctggttta gctttagcca
     1441 ttacatcgtg gaaagctttg aagttttcaa cgagtttgtc agcgtcaaat gatactttac
     1501 cgataagagc ttgtacgata cctgctttgt cagcacggta agtgatttta ccaccttttg
     1561 actcttcaac tgctttagca acatccattg ttactgtacc agttttaggg tttggcatca
     1621 agttacgtgg accaagtaca cgaccaagac gtccaacaac agccatcata tctggagttg
     1681 cgataacaac gtcgaagtcc aaccaaccac cgttgatttt agcaacgagg tcatcttcac
     1741 caacaaagtc tgcaccagca gcttttgctt cttctgcttt agcaccacgt gcgaaaacaa
     1801 gaacacgtga agttttacca gtaccgtttg gcaataccat tgcaccacgg atttgttggt
     1861 ctgctttacg tacgtcgatg ttcaagttgt aagcaacttc aactgacgca tcaaatttag
     1921 cgaagttagt ttcttttgca agagcaacag cttcttctac gctgtaaagt tttgtgctgt
     1981 caattttttc aagagcagca cgcaagtttt tgcttttttt agccatgtta tatgtctcct
     2041 tgtaatcaga taatcttggg tgctacttag tcagtaacag tgaatcccat agaacgagca
     2101 gtaccttcga tcatacgcat tgcagcttca agagatgcag cgttcaagtc aggcatttta
     2161 gtttcagcaa tttcttgtac ttgtgcacga gtaactgttg caactttagt tttgtttggt
     2221 gtacctgaac ctttttcaac acctgcagct tttttcaaaa gaacagcagc tggtggtgtt
     2281 ttagtgatga aagtgaatga tttatcttca tacacagaga taactactgg gatgatcata
     2341 ccagcttggt cagctgtacg agcgttaaac tcttttgtga atcccatgat gttgatacct
     2401 gcttgaccaa gtgctggtcc gactggtgga gctggagtag ctttacctgc aggaatttga
     2461 agttttacga gtttttcgac ttttttagcc atgatataaa atcctcctat tgtggttttg
     2521 gcggtaatta aagattttta cctcccacaa atatgtgttt tacacatacc ttttcattat
     2581 acacgaattt caaagaaatg caagctttag gtttactttt tttcacattt tttatagaat
     2641 gaatgtatgt ttgaatcgat tatattttta aaaatagtag cctttctatt catctttcta
     2701 accttggcca tttcgataat tgctgtcaag ttttttaggt tgcaaaaccg aggatggaac
     2761 tttgcagaca ttgcctttcc tctctatgca attgaattct atctgatttc ggacaaggcc
     2821 tactataaca gtctactgcc tcagcttgtc ctagccctct cactactagc gattggtctt
     2881 tgcggtttct tccttttaaa gaaaaagaat tttttatatc gccgtttctt caaggttttc
     2941 tggcgagcta gttttatctt gacctttttg atgtatctag ctttggttat tgcggtcttc
     3001 actttaaaat cctaaaatca tccatccttt catagatggc ataataaatc cccattgcag
     3061 gcgcagtggg gattcttttt tatactaaat cagacaaggc gacgtagatg tatagtcgaa
     3121 tgaattagct ttcagacaag gaactgaggt gcaggttgct agcacagcct agtggctgtg
     3181 ctaggttgga gatataactt gcaaagcaag tcacttctgc agagtacggc aagccgaaag
     3241 tgacgatgta tcaaagttaa ttcaaatgac tatagataga actgacgttc atcaaatcaa
     3301 gtcaacagcg tatgattttc taagagtatt aattagttgt catattgata ggcctctctg
     3361 ggcatgaaaa gatggactta ttcatgaatt tctagaggga ggccatctgg atcaaagaag
     3421 aaggccattt ttttaccatc aaaatcatct gttcgcagga cttcatgtcg gatgtccaaa
     3481 cggtcaaact cttccagaca agcttccaca tctgcaacct ggaaggctag atggcgtaga
     3541 cctgtatgtt caggattggg catggccgga cgcattggcg catctggttt gatgaagatt
     3601 tccaagatca gattgccttt tttgacattg aagagaatat catttttttc tgggcgaata
     3661 tgctcatcca cctgttcaaa gcctagtttc tcaacataaa attctcttgt tttgtcatag
     3721 tcactaccga taattgcaat atgatgaact gcatcaaatt tcattattgt gtttccaata
     3781 cctttctagg ttttgttcct tcagctggac caatgacacc cgctgcttct agctcttcca
     3841 tgaggcgtgt tgccctgtta aaaccaacag acaagcgtcg ttgaatcata gaggcgctgg
     3901 ctttttgtgt ctcaactacc aaggcacgtg cttcgttgaa aagtggatct cctccagtat
     3961 caccaaaacc tgtatcgcca tcaccttctg ccacttcacc aggatcaaag gattcatcat
     4021 aatctgcatc tgcttgatcc ttgataaagc caactattgc ttctacatca tcatcggata
     4081 taaaggaacc ttgcaagcgg actggatggt tctcatcaat cggtttaaag agcatgtcac
     4141 cgcggcctaa gagtttttca gcaccgtttt catctagaat cgtccgactg tctgtaccgc
     4201 ttgatacggc aaaggcgata cgagatggta cattggcttt aatcaaacct gaaataacat
     4261 caacagatgg tcgctgggta gcaagaatca tgtggatacc tgctgcacga gccttctgtc
     4321 ccaagcgaat aatggcatct tccacttcct tgctggctac catcatcaag tctgccaact
     4381 catctacgat taccacaatg agaggaagtg gaatctgttt ttcttctgaa cggctattaa
     4441 attcttcgac cttcgcatta taaccttcca agtttcggac accaatttgg ctaaacaact
     4501 catagcgttt ctccatttca tccactactt tttgaagagc tcgagcagcc ttgcgtgggt
     4561 tggtcacaac gggaatcaat aggtggggaa tatcattata gaccgataac tcgaccattt
     4621 tggggtcaat catcatgaac ttaacttggt ctggaccagc tttcatgaga attgaagaga
     4681 tgataccgtt gacagcaaca gacttccctg aaccagttga accagccacc aatagatggg
     4741 gcatacgtgc taggttaaag gaacggacgg aaccattgac agccttaccg agaggaattt
     4801 ccaaaagctt gtctgggtct gttttagatt gttcccagag ctcacggaaa ggtactgtag
     4861 ccacttcgga gtttggaact tcaataccga ccagagattt tcctgggata ggagcttcaa
     4921 tacggacatc tttagccgca agggctagag ccaaatcatc agctaggtta gaaatccgat
     4981 tgacacggac gccgactgct ggtttgactt catacttggt taccgatggt ccaatctcag
     5041 cacgctcaac tactaccttg attccaaaac tggcaaaggt gtcttctaaa actttaatat
     5101 tctgacgaac aattcgtttt tcattggact gactcttagc ttttatagga gcaaataagt
     5161 ctatggacgg aagtttatat gctagtctct gcttaggctt gaaatcaatc tgcacatcta
     5221 tctcgtcctt ctcaaactca tttggttcag aagtctggct tgccgagttt gattgtgctg
     5281 gttggtcatc ttctgccaac ataataggaa attcatcttc tatgtctaag tggtcatatt
     5341 ctgaaataat cggtacttga acaggctctt cttcaaggat ttcacccgtt tctaaatcga
     5401 catgtctatc taccagtgcc aaagcccgag ctgactcttc ctcttcctct agacgaaggc
     5461 gttcctcttc tagacgagct aattcttgct gacgtttttc ttctcgttca gccttcttct
     5521 gtgttcgaag ctctgctctt tttacctgcg tctccaccaa cttatctttg gcaaccgcca
     5581 agccatcggc aacatcataa acagaccatg ggcttacaaa aaagatacca aatgctatca
     5641 ctagaagacc gatgaagaag gaaccgacat ttgcaaagag gaaggagact ggcagataga
     5701 agatacttcc aatcatccct ccgcctagaa atgctgttac ctgaaactta gctaggtctg
     5761 acaaggccaa ttttagagta tgaccgaata agtcactccc ttgataggtc cagtctaagt
     5821 aagcctgaaa ttcaattaat aacccgataa caatcagcca gaagcccgat atggtccctt
     5881 ctctttcgcg taagactttg ggaataagaa ggtagataaa tgcacccgcc aataatagat
     5941 aggccagact accaaataat agccggaaaa tattgtagct ggtcactcca aacacaccta
     6001 ggcgactggc tgcaaaagca atgaataaaa gccccaatac tcgaagggtg atatttttaa
     6061 cacgttcttg ctgtgctaat tctgccttgg ttggtcgcct cgtagccttt ccttttttac
     6121 ttgtctttct catagatacc tcaactttct caatctcttt attctatcat aattgggaag
     6181 aattttcatg atagaaaaaa ccctgcaaat acagggtttc tcttggagtg aaagaataat
     6241 ttaagagggc aattaatatt atagagcgtt tagaataatc tgaccttcta cgtagaaggt
     6301 tgatgtcaag gtttcttgac catcattaat aaagatttcc aaacagttgg tgtcaaccaa
     6361 aacctctagc tctgttgctt gaaccgtgac cgctcgacga acagttgacc attcctcctc
     6421 accaacttgc tggattttga gagagctacg gttgatatag acttcttgtt tggctttatc
     6481 atagccaaat tgaagatagt cttggtcgtt tccaagtttt agttccaaac tgtcttccag
     6541 tctaagagag agcttgcttg gcccttggat actttgtcca atttcaatat ccttgaaggc
     6601 ttcaaagaca cttgttggca gaacttggtg caatccattt tcagtcttct tgagaatgcg
     6661 tggaagggtc atttgaccat accacttatg gtctaggtca ttggttacaa gcggacgtcc
     6721 ccatgtatgc atccaggcaa tcatgacacg ttccccttct ggaccttcag tcgtttgagc
     6781 tgcatagaaa tcatgcccat ggtcgatttc cttgaaggat tctgccacaa ataccttctt
     6841 gtcccagtcc acatgaccca tcacaaagat attggagttg atattgataa agtcattccc
     6901 gtctttttgg taacgcattg gagagataat caggtactct tgaccgtcca cttcaaagta
     6961 gtcaggacat tcccagacaa agccttggtt ggcctcacct ttcaagaaga tagactcaaa
     7021 tttccaatcc gtcaggttgg gagagctgag taagacgata cagccgacat tgtccttgtg
     7081 cttggttgcg acaacagagt agtagtgacc atctttctca aaaatcttcg gatcgcggaa
     7141 gtcattggca atcacttctt ctggcagtcc atctgctgtt gcgactggat tctgctcgat
     7201 tttttcaaag tgaataccgt cagttgagaa ggccatgttc tgaacttggc taactgtccc
     7261 gtcttcattg ttgatatgac cggtatacat gagccagagg acatcatctt tgacaatggc
     7321 agagcccgag aaacagccgt ctttatcgta agctttatca ggagctaggg caactggaag
     7381 ctgttcccag ttgaccaagt ccttactttt cgcatgcccc cagtgcatcg gtccccaaac
     7441 gctttcgtaa gggttgaatt ggtagaacaa atggtattct ccacggaagt agataaatcc
     7501 attggggtcg tttatccagc ctgtttctgg tgttaaatgg gcttgcggtt tgaaaatcgg
     7561 attgacgttt cctttttctg tctgaataaa ttgattggct tctactactg ttttcacagg
     7621 cagcctcctt ttgattactg attttcttta tactgatcgt agtatttttg cttgatagct
     7681 aaccagtcag aaagaccata gctttccaat tctttcaagt agctatccca ctcttcttcg
     7741 ataccgccgt ttacaatcca ttctgcacgt ttacggtaga tgaaatcgtt catatctgct
     7801 tcaacttggg caatcttgtc caaatcttct tgttccatga agacacgagg gtagatgtca
     7861 ttgttggtca catagtcaag ataggttgcg tgcatcaagt ccaaacgcca tttcgcatcg
     7921 tctggcatgg ttgttacagt accatagtat tcatcaagaa tagccagtgg tccaccaact
     7981 tctgtcttct gacgcaattc gcccggtgca gtaccgttca acggcaagtg tttcaagcta
     8041 tctgtagctg catcatattc gaagatgttt tgttgggttt catcaccata ggtaccccag
     8101 ttgttttgaa cagactggat tggtttgtat tgggcatcaa tccatttagc agtcaattcc
     8161 aagtttttat tagctgatgt gatgaccatg cggtcacgtt ggaagcccat accgtttgta
     8221 cgagtgatat gcttggttcc atcaggacct gccaatactg gcaagatgtc ataggtctca
     8281 ttcataccag tgatgttggc cttgtcccat gtgaagtaca caccatagag gttttcactt
     8341 ccttttgcca agtaggtatt ccagtcttgc tcaaaggctt ctgggtcaat gaggcctttt
     8401 tcttgaaggg tacgcatgaa ctcaacacct tctttgtaat cttcattgtc cgctgtaaag
     8461 tcaacggtcc cgtcattgtt tacgacgata tggtcatcgt tgtcaccgac accgaaggca
     8521 ccaaagagaa tcttgaagtc ttcgccgccg ttaccgttga caaaggacat tgggatttca
     8581 tccgccttgc catttccgtt tggatcttgg gtcttgaagg cttcaaggac cttaatcaag
     8641 tcatccgttg tttttggcat ttcaagaccg agtttgttca accattcttt gttgatccaa
     8701 gcaatatcgt taacagtgtg gattgattcc ttaccttcgc ccaattcttc aatccatggg
     8761 aatgagtaga tatggccgtc tggagccgta atcattgaac ggtattctgg attttcatcc
     8821 aaaattttct taaggtttgg catatgctta tcaatcaagt cttcaactgg tacgataacc
     8881 ccttgttttg cccagttcat caattccaca tcagaagccc cgtcgttatg gatggcatct
     8941 ggcaaatcac ctgaagcaat gtccaagttc cgcttttcac caaagtctga ctggtagttt
     9001 gtccaatcga tatgcacacc tgtttcttcc tcaagacgtt ggaaaatcaa cttatcattt
     9061 gggtctgctg gagctagggg ggagctagct gtcataaact taagcgtaac tgatttttca
     9121 agcgggaatt gaacatcttt caattcataa tctgggcttg aagcagtatt ttttgagcca
     9181 caagcagcca ataaaacggt acttgctaga agcgtaacaa ctgtttttcc aaatttgtgt
     9241 ttcatcagaa acaacctcct atatttgtaa attgattttt tagaatatct ttcagtttcc
     9301 ttttattact aggcaacgag ctgaaggctg tactgaagta cggcatagcg agttcaaata
     9361 atacatgatt atttgaagct ggagataagg aaacgaagtt tcctctttcg tcgacgtaag
     9421 aaaagagaaa atgaaagata tttattaacc ttttaatgaa ccagccatga tccccttgtc
     9481 aaagtatttc tggaagaatg gatacatgac aatgagtggc aagctagaga tgacaatggt
     9541 cgcatacttg atcatttctg cgattcgttt catttcattc atggcagcct gggcaccaat
     9601 catgtcctta gcaggctcgc tttgaatgag gattttacgt agcacaagtt ggagtggctc
     9661 caagtctggg tccttgatgt aaatcatggc atcaaagtaa gaattccatt gaccgacaaa
     9721 ggcatatagg aaaagtacaa acatgattgg cttagctaat ggaatcatga ttttccagaa
     9781 aatctgaaat tcattggcac cgtccataac cgctgcttct accaactcat ctggcaagcc
     9841 ttggaaatag gtccgtgcca agatgatatt ccacacattg actgcaccag gaataataat
     9901 ggcccagaca gtattcaaca ttcccaaatc tttaaccaag agataggtcg gaacaagtcc
     9961 gccaccgaag aacatggtga aaacgagaaa ggcattaatc cattttttcc cgaccaaatc
    10021 ttttttagag agcgggtagg cagtcatgac tgagatagcc acggtcacga aggcaaagga
    10081 gaaggaataa aagagggagt tgaaaaatcc gcgtaggata gacttgtctg aaaatacccg
    10141 ttgataacct tctactgtcc agtcctttgg attaaagcta atgcccttgc tgaccaaagc
    10201 ctgcggatcc atgaaggaag cgactaggat atagaccata ggcacaactg ttatgagaac
    10261 aagaaatgcc agcaagattt tattgacaat cagcattcgc ttgtcaaatt ttgtgtacat
    10321 ttgtgtattc atacgaacct cctataagcc ttgtccgtcg ttaatgcgtt tgacaatctt
    10381 attaactgca atcagcagga taatattgat aagtgcgtta aataagccaa ccgctgttga
    10441 ataagagtag tcacctgaca ccaaaccgat tttatagaca taggttgaaa tgatttcaga
    10501 actggttagg ttgagggagg tctgcatcaa gaaggctttt tcgtacccca cgttcatgat
    10561 acctccagct gctaagataa actgaatgac cataactggt tttagtgctg gtaagtcaat
    10621 atgccagatt cgttggaaaa tatttgcacc gtctagctta gccgcttcaa tcaaggctgg
    10681 atcaacattg accaaggtag ccgtgtaaag agtcgatgcc cagcccatta cctgccaaat
    10741 accactgagg atgtagaggg gtctgaagaa gtctgggtct gtcaggaagt tggcttcaat
    10801 gccaaacata cctaggatgg aattaaccgg tcctcctact gagaagaaga ggaaaatcat
    10861 accgacgatg acaacgactg agataaagtt tggagcgtag agcaccaact gaatccgttt
    10921 tttagcctta tcgctcaata gttgattgag catgatggcc aaaataattg gtggaagaaa
    10981 tcctaagagc agaccgtaga cactcaattt caaggtgttg gcaagtaagg tgccgaaatt
    11041 tggagaggaa agaaacttgg taaactcttc aaacccaatc cactcactac caagcacccc
    11101 ttttaaagga ttatagtctt taaaggcaat cagtactccg tacataggaa tgtaacggaa
    11161 gatgaaagtc aagaccaagc ctggtagtaa catcagtaac aagagttttt gttgcttgat
    11221 tcgatccacg agcccagctt gttgcagttt tgatgttttc ataagaactc ctttttgaaa
    11281 cgtttcaaat ttattgattt tagaaagagg atgtgaacct ctttttgaaa cgtttcattt
    11341 ttcttgtaaa aataaaacca actgtttatt gatgatttta ttttatcatt gtaaccgttt
    11401 tctgtcaact acttttttaa aatttttttg tagtttttcc ttcaagatag gaaatcggta
    11461 gagtgacttg tagggggcgc tctcttttct caataatatc aattagacag gcaacggctt
    11521 cctgggccat agactccaaa ggttgacgaa tggtcgaaag ggctaaagat ttttcacttg
    11581 ccaactgaat accatcatag ccaatcacct gcacatcttc tggaacccgt cgtcccagtt
    11641 tttccaaaac agcaagtgtg tctagggccg taaaatcatt gatggtgaaa atggcgtcaa
    11701 cctgtggatt gttaatcaga aactcttcga cttgaccaac aaagtcatca tatggctctt
    11761 ccaagtccaa aacatgacaa ggaaaacctg cctctaaaat ccgcttttca aaatacaatc
    11821 tacgcttctt ggtttcgttg atggttttat tgtgcccacc gataaaggca aaatgacgac
    11881 caccttttgc aatcaaggta ttcgctgcca gttccgcacc agctcggtta tccgaactga
    11941 cacaggctat gtccttgttt tcataaacac gatcgatact gacaaatgga atgttggagg
    12001 acaggtaatc ttcaatcggg ctataagtga tagcaataat gccgtccacc ttattttgtt
    12061 gcagcatagt cagataatcc agttcccttt ttggaccaga aatattgcaa aggagcaact
    12121 tgtaattttt cttactcagt tccacttcaa catgataggc aaattctcca aaaaaaggat
    12181 gccaaactgt cggaataatc aaggcgatgg tttcggtttt gttcttcttc atcccacgag
    12241 cataaacatc tggaatgtaa tttaattccc gaatggctgt attgactttt tcaagcgtac
    12301 tttccttaat acctggttca ttgttgataa ctcttgatac tgtcccaaca ctgacccctg
    12361 caactcgagc tacatctttc atagtaattg atttttgttc ttccatggtt tacccctttc
    12421 ttttgaaacg tttcaataat ttatactaga atatcattga tagcgttttc tgtcaaggga
    12481 aatagactta ttagcaaata aaaacaccca aaaagctaga atgattccaa cttttgggtg
    12541 ccgttcaaaa atcttttcta ttttatttgt acatcttctt cattgggtgt tttgcaagta
    12601 cttcagggtg attctcgaga gcagctctgc ctttttccgt caaggcataa cctcggacgg
    12661 taaagtatgg agccagtttt tcttctgtca agttgtcctt gagggcagtg tccaggtctg
    12721 ccgccttcat cttggacagg cctgtcacac cctctgcttt gaggatattt ttcttggcag
    12781 tcgaagtgat gtggtccagc gaatcaaagg ccgattccac ataggcataa ccgtctgcga
    12841 tcaactgctc catgtgagca ggaccattga taccatacat atactcaaag tacttggagt
    12901 agggcgtcgt cgtttcaaaa gaacccaggc taatccgcca taataaaata atatcacctg
    12961 gcaaaattcc ttcttctagc cgaaccatat tgcgtttggg aacgggcttg gcctccaaaa
    13021 gccaggctgc caagtcacgt tcgggggaaa tatagggttt gacctcatgg tctttataca
    13081 tttcttcaac acgattctgg agtgacattt actttacctc acgaattgcg taataatttc
    13141 catcattgtc tgcaaagctt attaccttca tgaaaccaag gtccatgact ggattcgttg
    13201 tgacaccacg ttcaacaagt tcttcgtaag ttttggctac atctactgat gtcattaaga
    13261 ttgatggtgt acctaagttt aattctggct ccatctcagc aacctccgcc ttatcatgaa
    13321 gtacaaattg tacctcacta tctgatttag gtgcaataat gtaagaagtt tgggggcctt
    13381 gcacttgttt ttctacacgt tcaaatccca ttttctcttt ccaaaattga gctgaagcct
    13441 caatatcatt cacatataac ataacttgtc cgattgattg aatcatctta aatacctctt
    13501 ttgcgttgta cgaacatgtg ttcaaaccat tatacactag atagggatat tctccaaaga
    13561 aaaacgactc ttagagccgt tcaaaaccta ggaaacgagt cccctgaaaa tgcagggtgc
    13621 ctacatctcc ctctgctaaa agggcatatt catctgaacg aaggcgaaat tcctgccgtt
    13681 ttccctccca ttcaaacgtt acataataat tggttcgtgc tacttcgtta ccaaaaacat
    13741 gggttcgttt agagacaagc ttagccttgc aagattcttt gggcgcccga ttgttgtggt
    13801 gccattccat aaaattttta atgatatgga aacaaaccgt cgctaatatg attgcggaaa
    13861 aactgtagaa aagggccgta tgccatacta ggcttccatc tggggcaaac aagttctcaa
    13921 acatcattat tctccttttg catctgtaaa tatagtatat catattgttg ttttagatac
    13981 gctttttata ctagataaaa attgaaaaaa tccatatttc atggtagaat gagcataatt
    14041 ttgaacaaga caagaaagga ttttcaatga aaaaaatact agttcttgct ctagcttcga
    14101 gcatcttgct aactgcctgc acaaacaact cgacagctaa tagctcatct tctacagcat
    14161 ctagctctag tccaagctcg actacgacag ctgatacaac tgcttctagc caatatgcca
    14221 aagatttggc atacgccatt aacaatccca aagctacatt cccccaactt tccaatgaaa
    14281 ttggtgaaaa tgaggctgct gttaaaataa aaacaaccga aggcgacatc actataaaac
    14341 tcttccccga gcaagctcca ctaactgtgg aaaactttct gactcatgcg aaggaaggat
    14401 actataatgg aacaatcttc catcgtgtca ttaaggattt catgattcaa ggtggcgatc
    14461 ctcttggaaa tggtactggt ggagagtcta tctggtccgg aaaagatact actatcgatg
    14521 ctgggaatgg ttttaaagac gagatttcag ctttccttta taacattcgt agctcacttt
    14581 ctatggcaaa cgctggcgcg ggaacaaatg gaagtcagtt tttcatcaat caaaatacaa
    14641 cagacatgtc tagccaactc tctagttcta gctatcctgg taaaatcatt gacgcttata
    14701 aaaacggagg caatccaaac ttagatggca agcataccgt atttggtcaa gtcattgaag
    14761 ggatggacat cgtggataaa atcgcttcag tcgaaacaga caatagcgat aaacctaaaa
    14821 cagatgtaaa aattgagtct attgaaattc tgaaagatta caccttttaa aaatggg
//
LOCUS       DASFSW010000041        14860 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.041, whole genome shotgun sequence.
ACCESSION   DASFSW010000041 DASFSW010000000
VERSION     DASFSW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14860
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.041"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..2588)
                     /gene="essC"
                     /locus_tag="U1099_000925"
     CDS             complement(<1..2588)
                     /gene="essC"
                     /locus_tag="U1099_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEM4867259.1"
                     /translation="MAETVVFYKDGFRYAADVSAGKKILFQGTEKAQVFVSSLDTPLT
                     IWLDGVQVCYDYDGVEGNLVSGMSLAGGVVYLLPSEQVILDPLDKQELTIGQHVGNSF
                     VLAGSSCHVLLKRSDQSWMLYRLAGSIYINNLLMEKGEMELALGDELAFEDTFFKFYA
                     DEVLVAGPVEASDELARKSASRYAFYEDYPDYHRSPRIIYRSSEDRVAINAPSNAPSK
                     PSDSLLKLILPPLMMVGITLVIMIFQPRGLYVLATIAMSIVTLGMSIAGYIKGRKDYQ
                     KELRDREGLYHDYLADKAKELAGLTKSQKDGQLYHYPAIETLVDLADSYHHRIYEKTP
                     LHFDFLYYRLGLGEVPVSYDLSYAQTERSGKRDPLELEGFQLYEQNKTISDMPIVANL
                     SHGPVGYIGPRALVIEQLQLMVNQIALFHSYHDVQFITIMPEEEKEQWDWMRFLPHAT
                     LQDMNVRGFVYNQRTHDQVLNSLNQILKLRRAQKEDKSNRESTLFSPHYVVLVTDEKL
                     ILDHVIMEFFTEDPTDLGCSLVFVQDVLSSLSENIKTIINIKDRNTGQLVMEEGQLRE
                     IDFALDHFPVGYDKETLVRRLAPLNHLQNLKSSIPETVTFMEMYGAETFEDLGVVSRW
                     EKHAPYKSLAVPLGLRGKEDIVYLNLHEKAHGPHGLVAGTTGSGKSEVIQSYILSLAV
                     NFHPHDVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRL
                     FATHDVNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKTNQPDFMKELVSTARIGRS
                     LGIHLILATQKPSGVVDDQIWSNSRFKLALKVADRSDSMEMLKTPDAAEITQVGRGYL
                     QVGNNEV"
     gene            complement(2592..3854)
                     /gene="essB"
                     /locus_tag="U1099_000926"
     CDS             complement(2592..3854)
                     /gene="essB"
                     /locus_tag="U1099_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEM4867260.1"
                     /translation="MKEETFVFGGQEFVFLKGDKEWHLDVKRSDVATQELSDLLLLDL
                     HHPQFLQQEMTVDEDTVHFTYHLDAFGQTFEDMKEKEVSEKLRLSLNVLELKQALKLP
                     ITFFVHPSSVFITKDGRAKIAYRAIPEKMVPQTLDATDLLKQLKSYILALFTEHDFMD
                     LYNGALDVVPVPEFLDTIRKAEDMDSLEGLLQGYFQDKLEEEKATLTRVSRSKHRLYR
                     YATIWLSALAGLLVLPLIYLVFMHNPFKEKMLDADTAFIKVDYPGVISELERVEVSSL
                     PYTQKYTLAYAYIQGFDLSNEQRSVILNNVTLKSDEQYLDYWIEIGRGNSQEALDLAK
                     RLDDSDLILYALYQQMEQVRENTTLSGEERETTLDELKAEYQKYWDTRTTILNEDGSQ
                     VKTGTSSTSSSTSSTESSSTSPSSSSGE"
     gene            complement(3856..4095)
                     /locus_tag="U1099_000927"
     CDS             complement(3856..4095)
                     /locus_tag="U1099_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEM4867261.1"
                     /translation="METHINVTLHDGGRIRDLRIPTHIEVRRLIKELDSIFEKENARK
                     KYQLHVVNKGFILDEGKVLADYPVTTGDIVEVLEV"
     gene            complement(4125..4589)
                     /gene="essA"
                     /locus_tag="U1099_000928"
     CDS             complement(4125..4589)
                     /gene="essA"
                     /locus_tag="U1099_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEM4867262.1"
                     /translation="MKKIALVLSCLVLFLPVGIEASELGDNSLQIDSSRIEVEKEKKI
                     GSQDLLVQSLFLAPDQAELTAKKEADQVLLEHQLNSLFQTGEKPSTDILQVGTLFDTR
                     THKVMTTKVDSGTEGAPAGLLSGLFFASIVVGILLLASLATYLMGKDEVGQE"
     gene            complement(4590..7739)
                     /gene="esaA"
                     /locus_tag="U1099_000929"
     CDS             complement(4590..7739)
                     /gene="esaA"
                     /locus_tag="U1099_000929"
                     /inference="COORDINATES: protein motif:HMM:TIGR03929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HEM4867263.1"
                     /translation="MDKQRIFKYGRNALLVLLLLLSIIGLNIAVQNNTSISKKEQEAG
                     QQNIKLDVALVNEDNTVTLNGTEYNLGASYAKNIEKNTAHNWSTVSRGTADKGLKEGH
                     YQLLVTIPSDFSTKILDINNTNVDKATITYKINANGNLQVENEAVKVAKGIIADLQSQ
                     LIDMYMASILGNLYQAQRNVQTLSDTKVTNIAVFRNNLYQTAVDFKTLFPNLTSTADS
                     TLAANNLLKEALSGNVTAYDSLNTAQTGLKDNLATLIEQRNQGKLSQEEFSASLLAMN
                     SSVLSAETDQLFATIKTTQEVLSGQLGAAAIDETGEASGYIGLVEKLDQAIADLQVAI
                     EAESTKLAEHEATISNFAETHLTTYYGDSLETIHIGAVLGKSTELPISLASYQTSLDQ
                     LILNQLAGLPSLTPADLTTDLNKLDPKAASQIRFDAAFAQAHFGEHFVASNDLKELQA
                     LAKELEPHLQAAADFKIPTPTTRATLSLEIPSGFDLVSWELNGNSYTNPSEVVALQKD
                     NTVTVTLQYEKSQTTTVSTTVVTDSTSTSSTTGSSSSEENTASTTAPSTGTPVTTTEM
                     AQGSVKVSVNNLEVTTAGFDWENFLKEQEAYRKAEVAYAAKVSEIISKYNQANRLLDQ
                     YYPVNTVTGERSSLTDSFFNQDAKSLLTSLVTSSITANLQSYSEARAQDQALKERLAE
                     LEGRKQELKDQLQYISTTNSDLAQQIANQLAFLEDVKARAQSIADGQEQQTALQGTHD
                     TELAALATELDASLSITGEVKSLSEQNVEEATNVNTVFTTFTNDVQAAQESGENLSSE
                     ATALMERFESELAENGDFVKTFTSVLSNTYDNGVPNEVILDFLSAPLTESSSSVKATV
                     NTYRPFTWILLLEMVALFAAYVFGTHRVIKAVKDKFKVSRLEDTDLVTVTVLSVLSLL
                     VGGLIGVVSSQQLQIDSELIPTWVLMITLFSLLLAQGQYLLIKNAKGLGMGISFFMVI
                     SFIYLSNAIGTTASLTGLPATLKAINPLSVLENSLSGYFDGEATSLTVIATVLVLGLG
                     FAALTAFVDVEKFLNKKED"
     gene            complement(7813..8103)
                     /locus_tag="U1099_000930"
     CDS             complement(7813..8103)
                     /locus_tag="U1099_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEM4867264.1"
                     /translation="MSRIKLTPDELRTSAIRYTEGSDNIDAILSSLSNEQSVIRENWE
                     GTAFDSFDAQFEALKPKIVEFSELLRDINAQLNKVADIIEQTDADIAAQING"
     gene            complement(8387..9199)
                     /gene="purR"
                     /locus_tag="U1099_000931"
     CDS             complement(8387..9199)
                     /gene="purR"
                     /locus_tag="U1099_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEM4867265.1"
                     /translation="MKLRRSERMVVISNYLINHPYELTSLNTFAEKYESAKSSISEDI
                     AIIKKAFEESSIGHIETITGASGGVVFTPSISKAESVEIAQALRDQMAESNRILPGGY
                     IYSSDLLSTPHILKNVGRIIADAFKDEKIDAVMTVATKGVPLANAVANVLNVPFVIVR
                     RDLKITEGSTVSVNYVSGSSDRIEKMFLSKRSLKAGSRVLIVDDFLKNGGTINGMISL
                     LSEFDSTLVGVAVFAENQKGDRSVANYKSLLAVTDINVKENRVDVELGNIFE"
     gene            complement(9301..10160)
                     /locus_tag="U1099_000932"
                     /pseudo
     CDS             complement(9301..10160)
                     /locus_tag="U1099_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            complement(10160..11101)
                     /locus_tag="U1099_000933"
     CDS             complement(10160..11101)
                     /locus_tag="U1099_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEM4867266.1"
                     /translation="MKINQMKKDEFFEGFYLIKTAEVRQTRAGKDYLALTFQDDTGEI
                     EGKVWDAQPGKIKDFTAGTVVHMQGRREVYNNTPQVNQLVLRLPKAGEPNDPADFKEK
                     PPVDVKDTKDYLSQMIFRIENATWQRIVRALYSKYDKEFYSYPAAKTNHHAFYSGLSF
                     HTATMVRLADKIGDIYLQLNKSLLFAGIMLHDLAKVIELTGPDNTGYTVRGNLIGHIS
                     LIDEEITKVLMELGIDDSLEEVTVLRHVILSHHGLLEYGSPVRPQIMEAEILHMIDNI
                     DAEMMMMLSALDKVGPGEMTNRIFAMDNRAFYKPNID"
     gene            complement(11091..12329)
                     /gene="rmuC"
                     /locus_tag="U1099_000934"
     CDS             complement(11091..12329)
                     /gene="rmuC"
                     /locus_tag="U1099_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEM4867267.1"
                     /translation="MDIVLLILLILVLLALVFLYGKWQSLALLLQDQAEDTADNLSDQ
                     LSYQLENATLKQQQAIHQEVERLRTELYQQLTDIRQELNRSHLETRDATDRRLQAIQE
                     SNEKRLKEMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVARDVGSLNK
                     VLSGTKTRGIMGELQLGQIIEDILTPSQYEREFATVSGSSERVEYAVKLPGRTEGDYI
                     YLPIDSKFPLADYYRLEDAYESGDKDQIDLHRKNLLAAIKRFAKDIQSKYLNPPETTN
                     FGVLFLPTEGLYSEVVRNPIFFDELRRQENIVVAGPTTLSALLNSLSVGFKTLNIQRS
                     ADDISKVLGNVKLEFGKFSDLLLKAQKQLNQASSNIDKLLTTRTNAIERSLRTIDLYE
                     DDQTKGLLGLSPLDEEDNEN"
     gene            complement(12330..12962)
                     /locus_tag="U1099_000935"
     CDS             complement(12330..12962)
                     /locus_tag="U1099_000935"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEM4867268.1"
                     /translation="MIKIAVIAGGSFDCFPEPADLYVGVDAGSLRLLDHFLPLDWAIG
                     DFDSVTSEELGRIKDQAERFLQAPAEKDDTDLELALKEVFKVYPQAQVRIYGALGGRM
                     DHMMANLFLVAEPDLAPYMEQIELVDSQNIVRFRPAGQHRLSPIAGMKYISFMPSDQS
                     RLTIRHAKYPLDASNYFFKKCYASNEFIDRDIDIQLDQGYVVLIYSKDKN"
     gene            complement(12955..13614)
                     /locus_tag="U1099_000936"
     CDS             complement(12955..13614)
                     /locus_tag="U1099_000936"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEM4867269.1"
                     /translation="MSHYKIAPSILAADYANFEKELKRLEKTGVEYVHIDVMDGHFVP
                     NISFGADVVAAMRPHSKLVFDCHLMVSNPENHIETFARAGADILTIHAEATVHLHGTL
                     QKIRAAGMKVGVVINPGTPLVTIEPVLNLVDQVLLMTVNPGFGGQAYIPEVAEKIADL
                     VKLREAKGLNFDIEVDGGIDDKTIHSAKNAGANVFVAGSYLFKGDLDANVAKLRAALH
                     D"
     gene            complement(13629..14504)
                     /gene="rsgA"
                     /locus_tag="U1099_000937"
     CDS             complement(13629..14504)
                     /gene="rsgA"
                     /locus_tag="U1099_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEM4867270.1"
                     /translation="MQGRIIKALAGFYYVEADGQIYQTRARGNFRKKGQTPYVGDFVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEQK
                     DMDPIIYISKMDLVEDRTEMDAFKAIYEKISYPFVYSLEELTPLLQDKVTVFMGQTGV
                     GKSTLLNRIAPELALETGAISDSLGRGRHTTRAVSFYNVFGGKIADTPGFSSLDYEVK
                     EAEALTDCFPEIAESSQDCKFRTCTHTHEPDCAVKEAVASSAISQSRFDNYLQFLSEI
                     QNQRETYTKVSKKFK"
     gene            14629..14710
                     /locus_tag="U1099_000938"
     tRNA            14629..14710
                     /locus_tag="U1099_000938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14662..14664,aa:Leu,seq:cag)
BASE COUNT         4164 a         3599 c         3014 g         4083 t
ORIGIN      
        1 acttcattgt ttccgacctg gaggtagcca cgacccacct gggtaatctc agccgcatct
       61 ggcgtcttaa gcatctccat ggagtcgctc cgatccgcca ccttgagcgc cagcttgaag
      121 cgggagttgg accagatctg gtcatccacc acaccagatg gcttctgagt agctaagatc
      181 aggtgaatgc ccaaactccg accgatacga gcggttgaga ccagctcctt catgaagtca
      241 ggttgattgg tcttgagctc cgcaaactca tcggagatca agaagagatg gggcatgggc
      301 tcagacacct cacccagctt gtacttcttc tggtattgat tgatatgatt gacatcgtgg
      361 gtcgcaaaga gacgttgacg acgcttcagc tccgcattga tggacactag ggcccgcatg
      421 gactgggcgc catccaagtt ggtgatggtc ccaagtaagt gaggcaggtc cttgaagagg
      481 ttagccatgc ccccaccctt atagtcgatg aggagaaagg caacatcatg cggatggaaa
      541 ttaaccgcaa ggcttaagat ataagactgg atgacctctg acttaccaga acccgtagta
      601 cctgccacca aaccgtgcgg accgtgagcc ttttcatgta agttcagata gacgatatcc
      661 tccttgccac gaagacccag aggcaccgcc aaactcttat aaggcgcatg cttttcccaa
      721 cgactgacaa cacccaagtc ctcaaaggtc tcagccccgt acatctccat aaaggtcacc
      781 gtttctggga tagaagactt aaggttctgt aggtgattaa gcggagctaa gcggcgcacc
      841 aaggtctcct tgtcgtagcc aactgggaag tggtccaagg caaagtcaat ctcacgcaac
      901 tgaccttcct ccatgaccag ctgaccagtg tttcgatcct taatgttaat gatggtcttg
      961 atattctcag acagagaaga cagcacatcc tgcacaaaga ccaggctaca gcccagatcc
     1021 gtcggatcct ctgtgaagaa ctccatgatg acatggtcca aaatcaactt ctcatccgtc
     1081 accaaaacca catagtgagg gctgaagagg gtcgactccc gattggactt gtcttccttt
     1141 tgagccctgc gcagtttcaa gatctgattg agcgagttga gcacctgatc atgggtcctc
     1201 tggttataga caaagccacg gacattcatg tcctgcaagg tcgcatgagg caagaaccgc
     1261 atccaatccc actgctcctt ctcctcctct ggcatgatgg taataaactg cacatcatga
     1321 taggaatgga aaagtgctat ttggttgacc atgagttgga gctgctcaat caccaaagcc
     1381 cgcggaccaa tatagccgac aggaccatgg gacagattgg ccacaatggg catatctgaa
     1441 atggtcttgt tttgttcata gagttgaaaa ccctccaact ccaagggatc ccgcttgccc
     1501 gaccgttccg tctgagcata ggacaggtca taagacaccg gaacctcacc cagacccaag
     1561 cgataataca agaaatcaaa atgaagaggc gtcttctcat agattcgatg gtggtagcta
     1621 tctgccagat ccaccaaggt ctcaatagca ggataatggt aaagctgacc atctttctgc
     1681 gacttggtca gaccagccaa ctccttggcc ttatcagcca gatagtcatg atagagcccc
     1741 tctcggtctc tcagctcttt ctggtaatcc tttctcccct ttatatagcc agcaatagac
     1801 atccccaacg tcacaatcga catggcgata gtcgcaagca cgtagagacc acgaggttgg
     1861 aaaatcatga taaccaatgt gatccccacc atcataagag gaggcaagat tagtttcaac
     1921 aaactgtcgg acggttttga aggtgcattc gaaggtgcgt tgatagccac cctgtcttct
     1981 gaacttcgat agataatccg tggagagcga tggtagtctg gataatcctc ataaaaagca
     2041 tagcgcgatg cagacttacg agccaactca tcactagcct ctacaggacc cgcaaccagg
     2101 acctcatctg cataaaattt gaaaaaagta tcctcaaaag caagctcatc tccaagagcc
     2161 agctccattt cccctttctc catcagaagg ttattgatat agatggagcc cgccaaacga
     2221 taaagcatcc atgactggtc actgcgtttg agcaagacat gacaacttga ccctgcaagg
     2281 acaaaactat ttcccacatg ctggccgatc gttaattcct gcttatcaag agggtctagg
     2341 atcacctgct ccgacggaag aagatagacc acgccccccg ccaaagacat accagataca
     2401 aggtttcctt ccactccatc atagtcatag caaacttgta ctccatctaa ccaaatcgtc
     2461 aaaggagtat ccagtgagga gacaaaaacc tgtgcctttt ctgtgccttg aaaaagaatc
     2521 tttttcccag cagacacatc cgccgcatag cgaaaaccat ctttataaaa aactactgtc
     2581 tctgccatag cttactcccc gctcgagctc gatggactcg tcgatgatga ctctgtagaa
     2641 gaggttgaac tagacgtcga acttgtacct gttttcacct gtgacccatc ttcattcaaa
     2701 atcgtagttc gggtatccca atacttttga tactccgctt tcaactcatc caaggttgtt
     2761 tcccgctctt ctcccgacaa agtcgtattt tcacgaacct gttccatttg ctggtaaaga
     2821 gcatacaaaa ttaaatccga atcatccaaa cgcttggcca aatccaaagc ttcttggcta
     2881 ttaccacgac caatttcaat ccagtagtcc aagtattgct catccgattt gagcgttaca
     2941 ttatttaaga taacagaacg ctgttcatta ctcaaatcaa acccttggat gtaggcatag
     3001 gcaagagtat atttttgagt gtagggaaga ctagaaacct ctacccgctc caactcacta
     3061 atcactccag gatagtccac cttgatgaag gccgtatccg catccagcat tttttccttg
     3121 aaaggattgt gcatgaaaac tagataaatc agtggcaaga ccaataatcc tgccagggca
     3181 gaaagccaga tggtcgcata gcgatagaga cggtgcttag agcgagaaac ccttgtcaaa
     3241 gtcgccttct cctcctccaa cttatcctga aaatagccct gcaaaagtcc ttccaaacta
     3301 tccatatcct ctgccttgcg aatggtatct aagaactctg gtacaggaac cacatccagc
     3361 gcaccattgt aaagatccat aaaatcatgc tctgtaaata aggcaaggat ataagatttt
     3421 aattgcttca acaaatccgt agcatccaag gtctgaggca ccatcttctc cgggatagcc
     3481 cgataggcta ttttggcccg accatcctta gtaataaaga cagatgacgg atgcacgaaa
     3541 aaggtgattg gcaatttcaa agcctgcttc aattctaaaa cattgagaga aagtcgtagt
     3601 ttttcagaaa cctctttctc cttcatatcc tcaaaggttt gcccaaatgc atccagatga
     3661 taggtgaaat gaactgtatc ttcatccacc gtcatttcct gttgtaaaaa ttgagggtgg
     3721 tgcagatcca acaagagcag gtcagatagc tcttgcgttg ccacatcaga ccgttttaca
     3781 tcaaggtgcc actctttatc accctttaaa aagacaaatt cctgtccacc aaagacaaat
     3841 gtttcttctt tcaccctaaa cctccaaaac ctctacaata tctcccgttg tcacaggata
     3901 atcggctaat actttccctt cgtccaaaat gaacccctta ttaacaacat ggagttggta
     3961 tttttttcta gcattctctt tttcgaagat gctatccaac tccttgatta atctcctaac
     4021 ctcaatgtga gtcggaatcc gtaagtccct gattcgtcct ccatcatgta aggtaacatt
     4081 gatatgcgtc tccatctcat ctcctccaat agaccactag aagattattc ctggcctact
     4141 tcatccttac ccatcaaata agttgctaaa cttgcaagca atagaatgcc aactacaata
     4201 gaagcaaaaa acaggccaga gagaagccct gctggagccc cctctgtccc actatctacc
     4261 ttggttgtca tcaccttatg ggtccgagta tcaaacaagg tccccacttg caaaatatcc
     4321 gtgcttggtt tctcaccagt ttggaatagg ctatttagct gatgctctaa taatacttga
     4381 tcagcttctt ttttggcagt caattctgct tgatctggcg ctaaaaacaa agattggacc
     4441 agtaaatcct gacttcctat tttcttttct ttttctactt caatgcgtga actatcaatt
     4501 tgaagactat tgtctccaag ttcggacgct tctatcccaa ccggaagaaa gagaaccaga
     4561 caactaagaa caagtgctat ctttttcatt taatcctctt ttttatttag gaatttttcc
     4621 acatcaacaa aagcagtcaa agccgcaaaa ccaagcccca atacgagcac cgtagcaatc
     4681 actgtcagac tggtagcttc gccatcaaag taaccagata aactattttc taaaacagaa
     4741 agagggttga tggctttcag tgttgcaggc aggcctgtca aactagcagt tgtaccaatg
     4801 gcattagata ggtaaataaa gctgataacc atgaagaagc taatccccat gccaagtcct
     4861 ttagcatttt ttatcaatag atactgacct tgtgctagta ataggctaaa caaggtaatc
     4921 atcagcaccc aagtcgggat caactcgcta tcaatctgca actgttgact tgatacaacc
     4981 ccgatcaatc caccgaccag aagtgataga acgctgagca cagtcaccgt tactaaatct
     5041 gtatcctcta gacggctgac tttaaactta tctttcacag ccttaatcac acgatgagta
     5101 ccaaaaacat aagctgcaaa cagggccacc atctccaaga gtaaaatcca ggtgaaaggt
     5161 ctgtaggtgt tgactgttgc ttttacagat gaagacgatt ctgtcaatgg tgcggacaag
     5221 aaatccaaaa tcacttcatt tggaacacca ttgtcatagg tattgcttaa gacagaagta
     5281 aaggttttta caaagtcgcc attttcagcc aattctgatt caaacctctc catcagggca
     5341 gttgcttcag aagatagatt ctctccgctt tcttgagcag cctgtacatc attagtaaat
     5401 gtagtgaaga cggtattgac attagtcgcc tcctcaacgt tctgttctga aagggatttt
     5461 acctcacctg taatagaaag ggaagcatcc aattcagtag cgagcgctgc aagttctgta
     5521 tcatgcgttc cttgaagagc agtttgttgc tcttgaccat ctgcaataga ctgagctcgt
     5581 gccttgacat cctccaaaaa tgccagctga ttcgctatct gttgagccaa atcactattt
     5641 gtcgtgctga tatattggag ttgatctttt aattcttgtt tgcgtccttc taactcagcc
     5701 aatctttcct tcaaagcttg atcttgtgca cgcgcttcag aataggattg taaattagct
     5761 gtaatggagc tcgtaactaa agaggtcaac agggatttcg cgtcttggtt gaagaaacta
     5821 tccgtcaaac tgctacgttc accagttaca gtattaacag gataatattg atctaataaa
     5881 cgattagctt gattatactt ggaaatgatt tcactaactt ttgcagcata agcaacctct
     5941 gctttacgat aagcttcctg ttcttttaag aagttttccc aatcaaatcc tgctgttgtg
     6001 acttccagat tattaacact taccttgaca ctcccctgcg ccatctcagt tgtcgtgaca
     6061 ggtgtaccag tggacggtgc cgtagtcgat gcagtattct cctccgatga gctcgaaccc
     6121 gtcgttgacg aagttgaagt tgaatccgtt actaccgttg ttgaaaccgt ggtcgtctga
     6181 ctcttttcat attggagagt gacggttact gtattatcct tttgcaaagc aacaacttca
     6241 ctaggattgg tatagctgtt gccattcaat tcccaagata ccaaatcaaa cccgctagga
     6301 atttctaagg aaagagtggc acgagttgtt ggagtaggaa ttttaaaatc agcagcagct
     6361 tgaagatgcg gttctaactc tttagcaaga gcctgtaatt ctttcaaatc attagaagct
     6421 acaaagtgtt caccaaaatg cgcttgcgca aatgctgcgt caaatcgaat ctgactcgct
     6481 gcctttggat ccaatttgtt taaatcagta gtcagatctg ctggagtcaa gcttggtaaa
     6541 cctgccaatt gattcaaaat caactggtct aagctcgttt gataggatgc tagagaaatc
     6601 ggcaactctg tactcttacc caaaaccgct ccaatgtgga ttgtttccag actatcgcca
     6661 tagtaagtgg ttaaatgtgt ttcagcaaag ttagaaatcg tagcctcatg ctctgctaat
     6721 ttcgtactct cagcttcaat tgctacctgt aaatctgcaa tagcttgatc caacttttca
     6781 accaaaccaa tatagccact ggcttcaccc gtctcgtcta tcgctgccgc ccccaactgc
     6841 cccgagagca cctcttgggt cgttttaatc gttgcaaaca actgatccgt ttcagcactg
     6901 agtacagagc tattcattgc taaaagcgaa gctgaaaatt cttcctgtga tagttttccc
     6961 tgatttcgtt gttcaatcaa ggtagccaaa ttatctttta gtcccgtctg agcagtattt
     7021 aggctatcat atgccgtcac gttgccagaa agcgcttctt tcaaaagatt attagctgcc
     7081 aatgttgaat cagccgtcga agtcaaattc ggaaaaagtg tcttaaaatc aacagctgtt
     7141 tgatagagat tgttcctaaa aacagcaata ttggtcacct ttgtatctga caaggtctga
     7201 acatttcgtt gtgcctgata aagattcccg agaatgctgg ccatatacat atcaatcagc
     7261 tgactctgta ggtctgcaat aattcctttt gccaccttaa cagcttcatt ctctacctgc
     7321 aaattaccat ttgcattaat cttatacgtg attgtcgctt tatccacatt ggtattgttg
     7381 atatccaaga tttttgttga aaaatcgctt gggatggtca ccaacaattg gtaatgacct
     7441 tcttttaggc ccttgtcagc cgtaccacga ctgacagttg accagttatg ggcagtattt
     7501 ttttctatat tttttgcata actagcgcca agattatatt ccgtaccatt tagggttaca
     7561 gtattatctt cattgaccaa ggcaacatcc agcttaatat tttgttgccc tgcttcttgc
     7621 tctttcttgg aaatactcgt gttgttctga acagcaatat ttaatccaat gatagataga
     7681 agcaaaagta agaccagcaa agcatttctc ccatacttaa aaatgcgctg tttatccatt
     7741 tttcatatct ccttcatttc gataaaaaat aggaaggctg gcgccttcct agcatttgaa
     7801 cttcgtcaac ccttagccat tgatttgcgc tgcaatgtct gcatctgttt gctcgataat
     7861 gtcagcaact ttattcaatt gggcattgat atctcgaagc aactcagaaa attcaacaat
     7921 ttttggctta agcgcttcaa attgagcatc aaaactgtca aaagctgtac cttcccagtt
     7981 ttcacgaata acagattgct catttgacaa agatgataaa atagcatcaa tgttatctga
     8041 accttctgta tagcgaatcg cagaagtacg gagctcatct ggggttaatt taatacgtga
     8101 catatcattc tccttttgtc ttataattac taatccatta tataataaaa aagccaaaaa
     8161 tacaaccata tatgcaactt tatttataga aaaaataatt atttagccag gtcaaaacat
     8221 aattgtaaaa attttatact attttgactg cagagcaaaa aaattgaagg aagatgtccg
     8281 aaacaaacct tcctccaatc tgagctgaaa attcagcttt ttctttatgc acgaataatc
     8341 taaataggga ccaccttaca tctctttaag tgcaggttgg taaatgttat tcaaaaatat
     8401 ttcccaactc tacatcgact cggttttcct tgacattgat gtcggtcaca gcgagcaaag
     8461 acttgtagtt agctacgcta cggtcccctt tttggttttc tgcaaagaca gccacgccta
     8521 ccaaggttga gtcaaactca gacaagaggc taatcatacc attgatggtg ccaccatttt
     8581 tcaagaagtc atcgacgatt aagacacggc ttcctgcctt caaactacgc ttagacagga
     8641 acattttttc gatacggtcg ctagatcctg acacatagtt gacagaaacg gttgaacctt
     8701 ctgtgatttt caaatcacga cgcacaatca caaacggtac attaaggaca ttggcaacag
     8761 cattggccaa agggaccccc ttagtcgcaa ctgtcatgac cgcatcaatc ttttcatctt
     8821 tgaaagcatc tgcaatgata cgaccgacat ttttcaaaat atgtggtgta gacagcaagt
     8881 cagatgaata gatataaccg cctggcaaga tacgattact ttcggccatc tgatcacgaa
     8941 gggcctgtgc aatttcgaca gactctgcct tagagatcga tggagtaaaa acaactcctc
     9001 cactggcacc agtaatggtt tcaatatgcc cgatagagct ttcctcaaaa gcctttttaa
     9061 tgatggcaat atcctctgag atagatgact tggcagattc atacttttcc gcaaaggtat
     9121 tcaaactagt taattcataa gggtggttga tgaggtaatt ggaaatgaca accatacgct
     9181 cacttcttct taatttcata aaatcatcct ctttcacttt tttctattat atcacaacaa
     9241 actacaaaaa gcgaactttt ttgattgaaa agtccgtctt tttcgtattt tgtttatcgc
     9301 ttacatatac cactttggaa gatgatgatt aaaatattca taccattcag aaatcttctg
     9361 ctcttgcaac ttcatcaatg gaatttgttc ggaatctact tgttctgctc aagaaagagc
     9421 gcctagacta ttactgttac ataaagagcc atgagtctcc aaaattcctc tggaatagcg
     9481 ccgtcaaaat aggagtctac ctgaccacgc gcaaaggctg gagacaaatc tgccgtgaaa
     9541 atcaaacggt tgaattcttc ccaggggtct cctatgtcgt agcgatcaaa gtctaatatt
     9601 ttcagtttcc catccgcacc cagtaggaag ttacctgtgt gaaaatctcc atgatggtaa
     9661 ggtctccctt caagcaagtg acgattggcc tgaacaaagt ctatcatggc ttgaccattt
     9721 ggataggaat gactagctgc ttggtagaca gctaatttat tgtcaatctt agcctgataa
     9781 aaactgttcc agtcgcgttg actttggtca atcggtaggg catgcaaggt tcgcaaaaac
     9841 tgccctgact gacagcccaa atcatataag acagaatcag acaagctagt agccacttca
     9901 ttcatgtcct gaccttccac ccattcatag agtgtataaa ctgactcctc acccacccaa
     9961 agagcgattg gctctgccac aggcaaacct agaccaaaca gatttttaac taactgaaac
    10021 tctaatcgct tagcctcata ggctggtcgc tctgctattc tcaaaagacc aaggcgacca
    10081 tcttctagcc ggaccttgta ctttcggtcc gtggaccagc ctttggtgag gggctgtcga
    10141 gacactatcc taactgccat tagtcgatgt tgggcttata gaaagcgcgg ttatccatag
    10201 caaagatgcg attggtcatc tcgcctgggc cgaccttgtc caaggccgat agcatcatca
    10261 tcatttccgc atcaatgttg tcaatcatgt ggaggatttc tgcctccata atctgcggac
    10321 ggactgggct tccatactcc aagaggccat ggtgactgag gataacgtgg cgcagaacag
    10381 tcacttcctc cagactatca tcgatgccca attccatcaa gaccttggta atctcttcat
    10441 caatcaagga aatgtgacca atcagattgc cacggacagt gtagccagta ttgtctggac
    10501 cagtcagttc gatcaccttg gctaggtcat gaagcatgat tcctgcaaag agcaggctct
    10561 tgttgagctg gaggtagatg tcaccaattt tatcagccaa gcgtaccatg gtcgctgtat
    10621 ggaaggacaa gcctgagtag aaggcatggt gattggtctt agctgctgga taggaataaa
    10681 attccttgtc gtacttgctg tagagagcac ggacaatccg ctgccatgtg gcattttcga
    10741 tgcggaaaat catctggctc agatagtcct tggtgtcctt gacatccaca ggtggttttt
    10801 ccttgaaatc agcagggtcg ttgggctcgc ccgcctttgg caaacggaga acaagctggt
    10861 tgacctgagg ggtgttgttg taaacctcac ggcgaccctg catgtgaaca acagtgccag
    10921 cagtaaagtc cttgattttc cctggctggg catcccagac cttgccttca atctcgccag
    10981 tatcatcttg gaaggtcaga gctaggtagt ctttgccagc tcgtgtttga cggacttcag
    11041 cagtcttaat cagatagaat ccttcgaaga attcatcttt tttcatttgg ttaattttca
    11101 ttatcttcct catctaatgg ggacaggcca agcagtccct tggtttggtc atcttcgtac
    11161 aggtcaatgg tacgaagact acgctcgatg gcgttggtac gagtggttag gagtttgtca
    11221 atattgctgc tggcttgatt gagctgtttc tgggccttaa gcaagaggtc agaaaacttg
    11281 ccaaattcca gcttgacatt gcccaagacc ttggaaatat catcggctga ccgttgaatg
    11341 ttaagggtct taaagccgac ggagagggaa ttgagcaggg cagacagggt ggtcggtcca
    11401 gctactacga tattttcttg acgacgaagc tcatcaaaga aaatcggatt gcggaccacc
    11461 tctgaataaa gtccctcggt tggcaaaaac aagacgccaa agttggtggt ttcaggcgga
    11521 ttgaggtact tgctctgaat gtccttggca aaacgcttga tagccgccaa gagatttttg
    11581 cggtggagat caatctggtc cttatcccca ctttcgtaag catcttccaa gcgataataa
    11641 tccgccaacg gaaacttgga gtcaatcggc agatagatat agtctccctc ggtccgtccc
    11701 ggtagcttga ccgcatactc cacacgctca ctagagcctg aaactgtggc aaactctcgc
    11761 tcatattggc taggtgtcaa aatatcctcg ataatctgtc ccagctgtaa ttcacccatg
    11821 atgccacggg ttttggtgcc agacagcacc ttattaagac tccccacatc acgcgccacc
    11881 gtctgcattt cacccagacc acgattgacc gattccaact gcttggacac cgtttcaaag
    11941 gaggcctgta ggcgggtttg cagggttttc tccaactttt cctcgactgt ttgccgcatc
    12001 tctttcaagc gtttttcatt tgactcctga atagcctgca agcgacggtc ggtggcatct
    12061 cgcgtttcca agtggcttct attcagctct tgacggatgt cggttagctg ttggtaaagc
    12121 tccgtgcgga gtctttccac ctcctgatga atggcctgtt gctgtttgag agttgcattt
    12181 tctagttgat agctgagctg atcagacaag ttgtctgctg tatcttcggc ttgatcttgc
    12241 aaaagcaggg ccaagctctg ccattttcca taaagaaaaa ccaaggcaag caagaccagt
    12301 atcagcaaaa taagtagtac aatatccatc taattcttat ccttactgta gatcaggacc
    12361 acgtagccct gatccagttg aatgtctatg tctctatcta taaattcgtt agaagcatag
    12421 cattttttga aaaaataatt gctggcatcc agcgggtact tggcatgacg aatggtcagg
    12481 cgactttggt ccgacggcat aaaggaaatg tacttcatac cagcaatcgg tgacaaacgg
    12541 tgctgacctg caggtcgaaa tcggacgatg ttctgactat ccaccaattc aatctgctcc
    12601 atgtagggtg ccaagtcggg ttctgcaacc agaaagagat tggccatcat atgatccatg
    12661 cgaccaccca aggctccgta tatgcgaacc tgggcctgcg gataaacctt gaagacttcc
    12721 tttaacgcta actctaagtc tgtatcatct ttttcagcag gagcttgcaa aaaacgctct
    12781 gcttgatcct ttatccgccc caactcctcc gaggtcaccg agtcaaaatc accgatagcc
    12841 cagtcaagag gcaggaaatg gtccagaaga cgaagagagc ctgcatctac ccccacatag
    12901 aggtcggcag gctcaggaaa gcagtcaaag gaaccgcctg caatgacagc aatcttaatc
    12961 atggagggca gctctcaatt tggcaacgtt ggcatccaag tctcctttga agaggtagga
    13021 tccagccaca aagacattgg cacccgcatt tttggctgaa tgaatggtct tatcgtcaat
    13081 cccgccgtcc acttcgatgt caaagttcaa gcctttagct tcacgcaatt taaccaaatc
    13141 tgcaatcttc tcagccactt ctggaatgta ggcttgtcca ccaaaacctg ggttgaccgt
    13201 catgagcaga acctgatcca ccaagttgag cacaggctca atggtcacaa gaggagtgcc
    13261 tggattgatc accacaccca ccttcatacc agcagcacga attttttgga gggtcccatg
    13321 caagtgaacc gttgcttctg cgtgaatagt caagatatcc gcacccgcac gtgcaaaggt
    13381 ctcgatatga ttttcaggat tggacaccat gagatggcaa tcaaaaacca gcttgctatg
    13441 aggacgcata gctgctacca catccgcccc aaagctaata tttggcacaa agtgaccgtc
    13501 catgacatca atgtgcacat attccacacc tgtcttttct agacgcttga gctctttttc
    13561 aaaatttgca taatctgccg ccaaaataga cggtgcaatc ttataatgtg acatacagaa
    13621 ccaactttct atttgaattt cttacttacc ttggtatagg tttcacgctg attttggatt
    13681 tcgctgagga attggagata attgtcaaag cgactttggg aaatggcaga gctggcaaca
    13741 gcttccttga ccgcacaatc cggctcatgg gtatgggtac aggtcctaaa tttgcagtcc
    13801 tgactgcttt ccgcaatctc tgggaaacaa tctgtcagag cctccgcctc cttgacttca
    13861 tagtctagag atgaaaaacc tggcgtatcc gcaattttcc caccgaagac attgtagaaa
    13921 ctgaccgcac gggtggtatg gcgaccacgc cccagactat ctgaaatagc acctgtttcc
    13981 aaggctaatt ctggcgcaat gcgattgaga agtgtcgatt tgccaacccc tgtctgcccc
    14041 ataaagaccg tcaccttatc ctgcaataat ggcgtcaatt cctccaaact atagacaaaa
    14101 ggatagctaa ttttctcata gatagccttg aaggcatcca tctccgtccg atcttctacc
    14161 aaatccatct tagagatata gataatggga tccatatcct tctgttcaag gagaactagg
    14221 aaacgatcca gtagattggc attaaaatca ggttccttgg ccgacatgat aaccaccgcc
    14281 tgatcgatat tgacgatagg aggtcgaacc aaactatttt tccgctcatg aatctttaat
    14341 atatagcctt ctgagttttc ctccgcagaa aagtccacaa aatcacccac gtagggcgtt
    14401 tgtcccttct tacgaaaatt tcctctggct ctggtttgat aaatctgtcc atccgcctcg
    14461 acatagtaaa aacctgccaa ggccttgata attcttcctt gcaatgaaga ctcctttgtg
    14521 tgtacttgct actattatac caaatttcct acaaaaaaac cgcaagcggt ttcgatttta
    14581 cattttcctc tagtcatttc aatgtaaaaa tagtataata aaaagcaagc ggaggtggtg
    14641 gaacggcaga cacgcatgct tcaggcgcat gtgcccatcg ggtgtgaggg ttcaaatccc
    14701 ttcctccgca ttatttataa aggataactc caagtcaaag ttgatgactt gggggcattt
    14761 ttatatcttc tcgctcatac tcaatgaaaa tcaaaaacag actagctcca aaggttttgg
    14821 aaacctttgg aggttggaaa tagagcgaac gtagttcgtt
//
LOCUS       DASFSW010000042        14807 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.042, whole genome shotgun sequence.
ACCESSION   DASFSW010000042 DASFSW010000000
VERSION     DASFSW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.042"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1063)
                     /locus_tag="U1099_000939"
     CDS             complement(<1..1063)
                     /locus_tag="U1099_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEM4867271.1"
                     /translation="MSTLDFDFTERLYANYLANPSLQATENAVSHNGLLKSLETRQSA
                     IDNDYVFSIDLTKDAVSNQKASGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFLEQIIATADQEIGSRKVKFLLDTPQQDGGQWDMVVALFEKYGVVPKSVYP
                     ESISSSASRELNQYLNKLLRQDAQILRDLLAKGASSKEVQIQKENLLQEIFNFLAVNL
                     GLPPRSFDFAYRDKDNVYHRNTNVTPQAFYEKYVGLKLSDYVSIINAPTTDKPYNKSY
                     TVELLGNVVGAPAVRYLNVEMNRFKELAIAQLKAGESVWFGSDVGQSSNRQTGIMATN
                     TYDFSSGLGIHFHQDKAGRL"
     gene            1197..2444
                     /locus_tag="U1099_000940"
     CDS             1197..2444
                     /locus_tag="U1099_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEM4867272.1"
                     /translation="MTKTPFVSKEVLETITEQFPTPFHLYDEKGIREKARALNAAFAW
                     NKGFKEYFAVKATPTPAILKILQEEGCGVDCATDVEVLMSEKLGFKDIMFTSNDTQAQ
                     EFVYARKVGATINLDAYEHIEFLKNVAGIPETVCLRYNPGGVFSLGTDIMDHPEESKF
                     GMTKEQLMKGYKELKELGVKEFGIHAFLASNTVTNDYYPVLAHQLFELALEIREETDV
                     TLDFIDLSGGIGVNYRPEQEPNDIAVIGEGVRKVYEEILTPAGMGHIKIFTELGRFML
                     APHGHLITKVLHRKETYRTYIGVDASAANLMRPAFYGAYHHITNITRPDAPIEVVDVA
                     GSLCENNDKFAVNRELPRAEVGDTLVIHDSGAHGFSMGYNYNGRLRSSEILLQEDGTA
                     RMIRRAETPEDYFATIYGFDFDR"
     gene            2547..2783
                     /locus_tag="U1099_000941"
     CDS             2547..2783
                     /locus_tag="U1099_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEM4867273.1"
                     /translation="MNLGLAILLIVLAFAGGVALGIYLSRKQVENYIADKPVLDENAL
                     RLMMSQMGQKPSEAKVQQVLRQIKSQQKVASKKK"
     gene            2873..3667
                     /gene="racE"
                     /locus_tag="U1099_000942"
     CDS             2873..3667
                     /gene="racE"
                     /locus_tag="U1099_000942"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEM4867274.1"
                     /translation="MDNRPIGFLDSGVGGLTVARELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKNVKMIVFACNTATAVAWEEVKEKLDIPVLGVILPGASA
                     AIKATQTGKVGVLGTAMTIQSDIYRQKIQALSPETQVDSLACPKFAPLVESNSHQSSL
                     AKKVVYETLRPLVGKVDTLVLGCTHYPLLRPIIQNAMGKDVKLIDSGAECARDISVLL
                     NYFQINRSRTEKDIQHRFYTTASPAAFKEIAESWMGIDIHVEHVEL"
     gene            3664..4674
                     /locus_tag="U1099_000943"
     CDS             3664..4674
                     /locus_tag="U1099_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEM4867275.1"
                     /translation="MTDKIYEYRDEHNWFIGKASFANLFGSFGENGREQEIYQIGQLF
                     DKLIAGNYEDENFNQCVNIEVIKLQSEFALFQFACDTLNELNNRQFKVLQHQGAILVT
                     ENDKLLLVHLPQAGVSTADFFGQDKGLASVGDTILIATKNEGKTKEFRKFFERFGYQV
                     ENLNNYPDLPDVAETGMTFEENARLKAETIAELTGKMVLADDSGLKVDALGGLPGVWS
                     ARFSGPDATDELNNAKLLHELAMVFELKDRSAQFHCTLVMAAPNRDSLVVEADWEGFI
                     GMDLRGEDGFGYDPLFLVGETGKTSAELTLEEKNKISHRAQALEKLVEAFPVWQEQAK
                     QS"
     gene            4647..5174
                     /locus_tag="U1099_000944"
     CDS             4647..5174
                     /locus_tag="U1099_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM4867276.1"
                     /translation="MAGASKTILVMSDSHGDRQIVEDIKHHYLGKVDAIFHNGDSELD
                     SQDSLWDGIQVVNGNCDYFGGYPDQLITQLGDVTIAQTHGHLYGINYGWQRLDYWAQE
                     VDADICLYGHLHVPDAEVRGKTLFLNPGSVSQPRGLVRECLYALVTIYDDHFHVDYYN
                     RQHQLYPALTKDISR"
     gene            5171..5629
                     /locus_tag="U1099_000945"
     CDS             5171..5629
                     /locus_tag="U1099_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM4867277.1"
                     /translation="MIAREFEVFLLDQEGTFLTPADKLAVIIDTHNIDHAKLLLSHMT
                     YSRVPVVTEDGRFFGTIGLTEIIKYQAENELSDDELNRDISVIVKTDEETVGLDYDLT
                     EVMRKLIDQSFLPVLGENREFLGIITRKSILKAINALLHNFPLASKEGKK"
     gene            5626..6357
                     /gene="xerD"
                     /locus_tag="U1099_000946"
     CDS             5626..6357
                     /gene="xerD"
                     /locus_tag="U1099_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEM4867278.1"
                     /translation="MKQAIESFIQSKKVSVNSQKSYTYDLQQFVTVTKGEISQQSLLV
                     YQQSLLDLKPAAQKRKMSAVNQFLYFLYENNLLDRFYKLQTMSEPASVKKKLEREDLT
                     LLFQESPWLDGQLITLLIALLGLTPSEIAELTSQQVNLDFQVLTVEKGGAKRVLTLPK
                     ELIPYMESHLSGRYVFDKKGQTYSRQWFFNRLTEFVQSIGKPDWTAQKLREQYILKHM
                     DEGKSLDQIAKQLGLKTSMSLEKFR"
     gene            6357..7076
                     /locus_tag="U1099_000947"
     CDS             6357..7076
                     /locus_tag="U1099_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEM4867279.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQVDIYEVPITEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMVIKSRRLLPKVVEQIDPEDDPEMDLLDQLEEYRKFKLL
                     SEKLGEQHDERANYFSKPKLDLIYDDVQLAKDKTIIDIFLAFSKVMAEKQASLRQSHA
                     TIARDEYKIEDMMDFVRSRFETGPRLELRQLFQESQDVNEMITIFLATLELVKVHEIV
                     LEQTETFGDIYLVRSEDESLS"
     gene            7060..7629
                     /locus_tag="U1099_000948"
     CDS             7060..7629
                     /locus_tag="U1099_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEM4867280.1"
                     /translation="MNRLAEIEVLLFVAGEDGLTLRNLAEMLEMQPTAVSQQLEKLSE
                     KYMADKASGLAILESSNRYKLVTKKEYANLLRIYAKTPINQTMSRALLETLSIVAYKQ
                     PITRIEVDDIRGVNSSGAISKLQAFDLIRENGKKEVLGRPNLYVTTDYFLDYMGINSL
                     EELPDVSEIDLIQEETELFVERNELENEN"
     gene            7619..8350
                     /locus_tag="U1099_000949"
     CDS             7619..8350
                     /locus_tag="U1099_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM4867281.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELGTVIKSG
                     DKVEVEGKPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVVDLLPTVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYLARVKGVANKEVLRPLTRGVVIDGKKTKP
                     AVYEIIKVDPVKNRSVVELTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGNLDLTGLRP
                     GEYRKLNKKDISKLHTLAVTKPVKK"
     gene            8350..8589
                     /gene="yidD"
                     /locus_tag="U1099_000950"
     CDS             8350..8589
                     /gene="yidD"
                     /locus_tag="U1099_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEM4867282.1"
                     /translation="MTRLLIGLVRFYQKFISPLFQPSCRYRPTCSAYMIEALQKHGLR
                     GFLMGLARIGRCHPFVEGGEDPVPDRFSLKRNKQK"
     gene            9157..9702
                     /locus_tag="U1099_000951"
     CDS             9157..9702
                     /locus_tag="U1099_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEM4867283.1"
                     /translation="MNIETLEYIEETAKNHIVLFEPQIPQNTGNIARTCAATNSPLHI
                     IKPMGFPIDDRKMKRAGLDYWDKLDVRFYDNLAEFMDYASKQGRVHLVSKFADKTYSD
                     EDYNDGNIHYFLFGREDKGLPEDFMREHPEKAIRIPMNDEHVRSLNVSNTACMIVYEA
                     LRQQQFAGLDLVHTYDGDKLK"
     regulatory      9752..9966
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            10026..10583
                     /locus_tag="U1099_000952"
     CDS             10026..10583
                     /locus_tag="U1099_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM4867284.1"
                     /translation="MTNTRKMTIIAILSAVSFLLMYLKFPLIPTASFLEVDFSLVPIL
                     FGLLVLDMKSSFAILLVRTFLKLILNNQGPSTIIGLPMNIAAMSVFILAVAYFWKNEQ
                     TLKNYLKAAAVATIGSTAVMLVLNYVYAVPVYAAFANFDIKEILGLTNYLLMMVLPFN
                     LLQGIVLSAIFYICYKAAQPILKKV"
     gene            10608..11261
                     /locus_tag="U1099_000953"
     CDS             10608..11261
                     /locus_tag="U1099_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEM4867285.1"
                     /translation="MKNKQIHLRNASFFALAFVILGYVVKFYPSTLAGFDSTVQSAIR
                     GNLPSGASQFWTSITVLGNTVIILAITLILAAIFYYYKKWKIEAYFLLASFAAMGVAS
                     TALKYVYQRPRPSIEWLIDTIGYSFPSWHTASTMMIVGAVVIIVHQRMKKGIARLVLK
                     AVLILLAVLVAVSRIYIGVHYPTDIIGGWLLAVTLLQMMYPFYDQKRFEWRFQSKQK"
     gene            complement(11485..13284)
                     /locus_tag="U1099_000954"
     CDS             complement(11485..13284)
                     /locus_tag="U1099_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEM4867286.1"
                     /translation="MKIKWLSVIRVIGLVFVLLYHFFIKYFPGGFVGVDLFFTLSGYL
                     TTALLIDEFAKHKKIDIVSFFRRRFYRILPPLVLTILLVLPLALLVRNDFIANIGNQI
                     AAALGFMTNFFEILSGGSYENQFSPHLFVHTWTLAIEVQYYLVWAGLVWYLTRISKSV
                     GQLRGSIFLTSISLFSLSFLAMSISSFFVDNFSTIYYSSFTHTFPFFLGSVLATIAGI
                     GNTTTAFQQQVNTLSIKQAIGLFLGGLGVELLLLFTLQFSSIWTYLIGFLLSSLATAT
                     MIFAARVLHEKTEDVKEPAIILFFASISYGIYLFHWPLLIIFTQLTNATIAAGLSFVF
                     STILSTISFYILEPYIAGKVGSLFGLPIDLKPYKKWLLGSFACLTIVGLGICLFAPKL
                     GSFEQESMVSNLYQAQTQLNTTRSAAENAKATSFEIQKGVIIFGDSVTVRASSAIQTA
                     LPDAQIDGTVSRNLSEIAKLINLYKQNNSLKETVVVALGTNTTDNYQELLDQLVKDFP
                     KGRRLIFVTPYDGNFTPSESISYQTGQYEKELAEKYDYISIADWYQVAKDNPPIWYNT
                     DLVHFNLESNGGEVFAQTIKNAVEAVADGPIKN"
     gene            complement(13274..13696)
                     /locus_tag="U1099_000955"
     CDS             complement(13274..13696)
                     /locus_tag="U1099_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867287.1"
                     /translation="MKEFKLPSIKWHDITSHFTRPKLEILSLIIILICALSVFTGRIA
                     SKQAMTFNNGALQYNGYVVANKMNGQGKLTFDNGDVYEGQFKNGIFHGQGTYTSASGW
                     VYTGQFKNGYADGKGKLTTEGQATYEGTFKQGIYQYEN"
     gene            complement(13828..14262)
                     /locus_tag="U1099_000956"
     CDS             complement(13828..14262)
                     /locus_tag="U1099_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEM4867288.1"
                     /translation="MKKIVFVCLGNICRSPMAEFVMKDMTSQFHIESRATSNWEHGNP
                     IYSGTQKIFQKHKIPYDKTKTSQQISQADFEEFDVIIGMDSNNVRDLQKMAPAHAQNK
                     IFQFAEKSVPDPWYTGDFDETYEMVKKGCQDWLDRLQSFDQE"
     gene            complement(14432..14644)
                     /locus_tag="U1099_000957"
     CDS             complement(14432..14644)
                     /locus_tag="U1099_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEM4867289.1"
                     /translation="MSEEKFDAKLEQLSGSAKEVIGKLTGDKEIEVEGLVEKGIGKAK
                     ELVEDAKDGLEGAINGIKKAFDKEEK"
BASE COUNT         4352 a         2801 c         3291 g         4363 t
ORIGIN      
        1 ctaatcttcc agccttgtct tgatggaaat ggatacccag acctgaagag aagtcgtagg
       61 tgttggttgc cataatacca gtttgacgat tgctactttg accgacatcg gaaccaaacc
      121 aaacggactc accagctttg agttgagcaa tagctagttc tttaaaacgg ttcatttcta
      181 cattgaggta gcggacagca ggggcaccaa caacgttccc caacaattcg actgtataag
      241 atttattgta aggtttatca gtagttggag cattgataat ggagacatag tcagatagtt
      301 tcaaaccaac gtatttctcg tagaatgcct gaggtgtcac atttgtattg cggtgataga
      361 cattgtcttt atctctatat gcaaaatcaa agctgcgtgg agggagtcca aggttgacgg
      421 ctaggaagtt aaagatttct tgaagcaagt tttctttttg tatttgaact tctttcgagg
      481 aagctccttt ggcgaggagg tctcgcaaga tttgtgcatc ttgacggagc aatttgttaa
      541 ggtactgatt gagctcacgg ctggcactgg atgagataga ttctggatag acggatttcg
      601 gtaccacacc atatttttca aatagggcaa caaccatatc ccattgaccg ccatcttgtt
      661 gcggggtatc caataagaat tttaccttac ggctaccaat ttcttggtca gctgtggcaa
      721 tgatttgttc gaggaaccag ttggattttt catatttatc ccaaaagaag gtatgggcct
      781 gtgatagctc aaaattctcc agtttgaaat cagagatgag cttgtggcgg aaggtgttta
      841 atgcagcaaa catccagcaa cgtccagaag ccttttggtt tgaaacagcg tccttggtca
      901 agtcaattga aaagacatag tcattgtcga tggcgctttg gcgtgtttct aaggatttca
      961 aaagtccgtt gtggctaacg gcattttcgg ttgcctgtag gcttggatta gctagatagt
     1021 tggcatacaa gcgttctgta aaatcaaaat ctaatgttga catatattac tcctttttta
     1081 atgataatca cattatagcg ctcataattt attttgcaag tatttggtgg agttgaaagc
     1141 tacagttcct cacatatttg tgatataata ggaaaaatat ttgatgaggt agtgacatga
     1201 ccaagacacc atttgttagt aaagaagttt tagaaaccat tacggagcaa tttccgactc
     1261 cttttcattt gtatgatgaa aaaggcattc gtgagaaggc ccgtgccctc aatgcagcat
     1321 ttgcatggaa caaaggcttt aaggaatatt ttgcggtcaa ggcgacaccg acacccgcta
     1381 ttttgaaaat tttacaagaa gaaggttgtg gagtggactg tgcgacggat gtagaagtac
     1441 tcatgagtga aaaattgggc tttaaggaca tcatgttcac atcaaatgac acccaagcgc
     1501 aagagtttgt ctatgctcgt aaagttgggg cgaccattaa ccttgatgcc tacgagcata
     1561 ttgagttttt gaaaaatgtt gctggtatcc cagaaacggt ctgcctccgt tacaatccag
     1621 gtggggtctt ctcgctggga acggatatta tggaccaccc agaagagtct aagtttggga
     1681 tgaccaagga acagttgatg aaaggctata aggaactaaa agagcttggt gtcaaggaat
     1741 ttggtattca tgcctttcta gcttcgaata cagtaacaaa tgactactat cctgtcttag
     1801 ctcatcaact ttttgaattg gccttggaaa ttcgtgagga aacagatgtg accttggact
     1861 tcatcgacct atcaggtggg attggagtca attatcgtcc tgagcaagaa ccaaacgaca
     1921 ttgctgtcat tggtgagggt gtccgtaagg tttatgagga aattctcacg ccagctggta
     1981 tgggacatat taaaatcttt acagaattgg gtcgctttat gttggcgcca cacggtcact
     2041 tgattacaaa ggtgctccat cgtaaggaaa cctatcggac ttatataggt gtggatgctt
     2101 cggctgcaaa ccttatgcgt ccagccttct atggcgccta ccaccatatc accaatatca
     2161 cgcgcccaga tgcgccaatc gaagttgtgg atgtggcagg ttccctctgt gaaaacaacg
     2221 acaagtttgc ggttaatcgg gaattaccac gcgcagaagt aggagacacc ttggtcattc
     2281 atgacagtgg tgcccacggc ttctccatgg gctacaacta caacggtcgt ctgcgttcct
     2341 ctgaaatcct tttgcaggaa gatggcacag cgcgtatgat tcgtcgtgct gaaacaccag
     2401 aggattattt cgcgaccatt tacggttttg attttgacag gtaagtcttg gaaaagcctg
     2461 ctaaatttgg tataatagat tcattaagag attgtcgcag ggcaattccg aatgaattat
     2521 ttttagaaga gtcaggagat tgacaaatga acttaggttt agcaatttta cttattgtat
     2581 tggcatttgc aggtggtgta gccttgggta tttacttgtc acgcaaacaa gtggaaaact
     2641 acattgctga caagccagtt ttggatgaaa atgctttgcg tttgatgatg agccaaatgg
     2701 ggcaaaaacc aagcgaggca aaagtacaac aagtacttcg ccaaatcaag agccaacaaa
     2761 aagtggcaag caagaaaaaa taatcaaact gggactggga tattcattcc cagatcccat
     2821 tttttgagta gaaaagaaag gctagtcaga ttttctggct attctcagta ttatggataa
     2881 tcgaccaata ggttttttag attcaggtgt ggggggatta acggttgcgc gtgagttaat
     2941 gcgccaactt ccccatgaag aaattgttta tataggggac tcggcacggg caccttatgg
     3001 cccccgtcca gcggaacaaa tcagagaata tacttggcag ttggtcaatt tccttttgac
     3061 aaaaaatgta aaaatgattg tctttgcctg caatacggca acagcggtag cctgggaaga
     3121 agtcaaggaa aagttggata ttcctgtttt gggagttatt ttaccaggtg cttcggcagc
     3181 tattaaggcg acgcaaactg gtaaggttgg tgtcttagga acggctatga ccattcaatc
     3241 agatatttat cgacaaaaga ttcaggccct ttctccagaa acgcaggtag acagcctagc
     3301 ctgtcccaaa tttgcaccac tggttgagtc caatagccac caatccagtc ttgccaaaaa
     3361 ggttgtttat gagacacttc gaccactcgt tgggaaagtg gataccttgg tcttgggatg
     3421 tacccattat cccctacttc gccccatcat ccagaatgcc atgggcaagg atgtcaagtt
     3481 gattgacagt ggggcagagt gcgcgcgaga tatttctgtt ttattaaatt attttcaaat
     3541 caaccgcagt cgtaccgaaa aggatattca gcacagattt tacacaacag ctagtccggc
     3601 agcttttaaa gaaatagctg aaagctggat gggcattgat attcatgtgg agcatgtaga
     3661 actatgacag acaagatata cgaatacaga gatgaacaca attggtttat cggaaaagcc
     3721 agctttgcca atctatttgg ttcatttggt gaaaatggca gagagcagga aatttaccag
     3781 attggtcaat tatttgacaa actgattgct ggcaattatg aagacgagaa ctttaaccag
     3841 tgtgtcaaca ttgaagtgat taaacttcag tctgaatttg ccctctttca atttgcctgt
     3901 gatactttga atgagttaaa caatcgtcag ttcaaggttc tccaacacca aggagctatt
     3961 ctggtaactg agaatgacaa acttctctta gtccatttgc cccaagcagg ggttagcacg
     4021 gcagatttct tcggtcaaga caagggattg gcaagcgtag gagataccat cttgatcgct
     4081 acaaaaaatg aaggtaagac caaggaattc cgtaagtttt ttgagcgttt tggctaccag
     4141 gttgagaacc tcaacaacta tcctgacctg ccagatgtag cagaaactgg tatgactttt
     4201 gaggaaaatg cacggctcaa ggctgaaacc atagcagagc tgacaggcaa gatggtcttg
     4261 gcagatgatt ctggtttgaa ggttgatgct ttaggtggtt tgccaggagt ttggtctgcc
     4321 cgtttttcag gcccagatgc cacagatgag ctcaataatg ccaagctctt gcacgaattg
     4381 gccatggtat ttgaactgaa agaccgttcg gcacaattcc attgtactct ggtcatggct
     4441 gctccaaatc gtgatagttt ggttgtcgaa gcggactggg aaggctttat cggtatggac
     4501 ctccgtgggg aagatgggtt tggatatgat ccacttttcc tcgtaggtga aacaggtaag
     4561 acctcggcag aattaaccct agaagaaaaa aataagattt cccaccgtgc acaagcacta
     4621 gaaaaattag tggaggcatt tccagtatgg caggagcaag caaaacaatc ctagtcatga
     4681 gcgactctca tggggataga cagattgtag aggatattaa acatcattat cttggcaagg
     4741 tcgatgccat ctttcacaat ggtgattcgg agctggatag tcaagatagt ctctgggatg
     4801 gtattcaagt ggtaaatggc aactgcgact attttggcgg ttatccagac cagctcatta
     4861 ctcaattggg tgatgtgact atcgctcaga ctcatggtca tctttatggc attaattacg
     4921 gctggcagcg gttggattat tgggcacagg aagttgatgc ggatatttgt ttgtatgggc
     4981 atctgcatgt gcctgatgcc gaagtgcgtg gcaagaccct cttcctcaat cctggctccg
     5041 ttagtcaacc tcgcggtttg gtcagagagt gtctctatgc tcttgtgacc atctatgatg
     5101 atcacttcca tgtggattac tacaatcgac agcaccaact ttacccagca ttgacaaagg
     5161 atatttcaag atgattgcaa gagaatttga agtcttttta ttggaccagg aaggaacttt
     5221 cttgacacca gcggataaat tggcagtaat tattgatacg cataatattg accatgcaaa
     5281 actgttactc agtcacatga cctattcacg cgtacctgtg gtaacagaag atggccgctt
     5341 ttttggaacc atcggcttga cagaaatcat taaatatcaa gctgaaaatg aactatcgga
     5401 tgatgagtta aacagagata tttctgtgat tgtaaagaca gatgaagaaa cggtaggtct
     5461 ggattatgat ttgacagaag tcatgcgcaa gctcatcgac caatccttcc taccagttct
     5521 tggagaaaac agggaatttc tggggattat tacccgtaaa tctattctga aagccatcaa
     5581 tgcgcttttg cataattttc ccttggcatc aaaagaaggc aagaaatgaa gcaggcgatt
     5641 gaatctttta tccagagcaa aaaggtcagt gtcaacagcc aaaagtccta tacctatgac
     5701 ctgcagcagt ttgtgacagt aacaaagggg gagattagcc aacagtctct tttggtctat
     5761 cagcagtctc ttctagactt gaaacctgct gctcagaaaa gaaaaatgtc agcggtcaat
     5821 caatttctct actttcttta tgaaaataat ctcctggatc gtttttataa attgcaaacg
     5881 atgtcagaac cagcaagcgt caaaaagaaa ctggagcgtg aggatttaac tctcctcttt
     5941 caagaaagtc cttggcttga tgggcaactg attactcttt tgattgcctt actagggctg
     6001 actcctagtg aaatagcaga gctgactagt cagcaggtca atctggattt tcaagtcctg
     6061 acagttgaga agggaggagc caagcgggtg ctcactctgc ccaaggaatt gataccctat
     6121 atggagtctc atctgtctgg acgctatgtc ttcgataaga agggacagac ctattctcgt
     6181 caatggtttt ttaatcgtct gacagaattt gtccagtcca tcggcaagcc tgattggaca
     6241 gcccaaaaat tacgggagca gtatattcta aaacatatgg acgaaggaaa atccttggac
     6301 caaatcgcca agcagttggg actgaaaaca agcatgagtt tggaaaaatt tagataatgg
     6361 atataaaatt aaaagatttt gaagggccac ttgatctcct gctacacctg gtgtccaagt
     6421 atcaagtaga tatttacgag gttccgatta cagaggttat tgagcaatat ttggcctaca
     6481 ttgctacctt gcaagccatg cgtttagagg tagcgggaga atatatgctc atggctagtc
     6541 aattgatggt catcaaaagt cgtcggttat taccgaaggt tgttgagcaa attgatcccg
     6601 aagatgatcc tgaaatggac ctcttggacc agttggaaga ataccgcaag ttcaagcttc
     6661 tcagtgagaa attaggggag caacatgatg aacgggccaa ttatttttca aaacccaaat
     6721 tagatttgat ttatgatgat gtacagttag ctaaggataa gaccatcatt gacattttcc
     6781 tagctttttc taaagtaatg gctgaaaaac aggcaagcct ccgtcagtct catgcgacca
     6841 ttgcccgtga tgaatataaa atcgaagaca tgatggactt tgttcgcagt cgtttcgaga
     6901 caggtccacg ccttgaactc cgtcagcttt ttcaagaaag tcaagatgtc aatgaaatga
     6961 ttacgatctt ccttgcgact ttagagttgg tcaaggttca tgaaatcgtc ttggagcaga
     7021 cagagacttt tggagatatt tatctagtaa ggagtgaaga tgaatcgctt agctgaaatc
     7081 gaagtcctgc tctttgtggc aggtgaggat ggcttgacgt tacgaaattt ggcagagatg
     7141 ttggaaatgc agccgacagc tgtcagccag cagttagaga aacttagtga gaaatatatg
     7201 gcagacaaag cgtcaggctt agccatctta gagtcttcca atcgctacaa gttggttact
     7261 aaaaaagaat atgcaaacct tttgcgaatc tatgccaaaa caccaatcaa tcagactatg
     7321 tcacgggctc ttttggaaac cttatcaatc gtagcctata agcaacccat tactcggatt
     7381 gaagttgatg atattcgcgg agtcaattcc agcggtgcca tcagtaaatt acaggccttt
     7441 gacctcattc gtgagaacgg taagaaagag gttcttggta gaccaaatct ctatgtgaca
     7501 acggattatt tcctggatta catgggaatt aatagtctag aagaattgcc tgatgtgtct
     7561 gaaatagatt tgattcaaga agaaacagag ctattcgtag aaagaaatga gttagaaaat
     7621 gagaattaat aaatacattg cccatgctgg agttgccagt cgtcgtaagg cagaagaact
     7681 aatcaaacaa ggactagtta ctgtaaatgg tcaggttgtt cgtgagctag ggacagttat
     7741 caaatcgggt gataaggtag aggttgaggg aaaaccaatt tataacgaag agaaggtcta
     7801 ctatctcctc aataaaccac gaggtgttat ttctagtgtg tcagacgaca agggaagaaa
     7861 gacagttgtg gacttgttgc caacagttaa ggagcggatt tatccagttg gacgtctgga
     7921 ctgggataca tctggggtct tgattttgac caatgacggt gattttacag acgaaatgat
     7981 tcacccacgt aatgagattg ataaggttta cttggcgcgt gtcaaaggag ttgccaacaa
     8041 ggaagttttg cgtcccttga cccgtggtgt tgtcattgat ggcaaaaaga ctaagcctgc
     8101 ggtgtacgag attatcaagg tggaccctgt taagaaccga tcagttgtag aattaacaat
     8161 ccatgaaggt cgaaaccacc aggttaagaa gatgtttgag gctgtcggct tgcaggtaga
     8221 taaactgtct cgtactcgct ttggtaatct tgacttgacc ggcctacgtc caggagaata
     8281 ccgcaagctc aataagaaag acatcagtaa actacatacc ctggctgtta ctaagccagt
     8341 aaaaaaataa tgactagact tttaatcggc ttggtccgat tctatcagaa atttatttcc
     8401 cccctttttc agccctcttg tcgctaccgt ccaacttgtt cggcttatat gatcgaggcc
     8461 ttacaaaaac atggactcag gggctttttg atggggttgg ctcgaattgg tcgctgtcat
     8521 ccctttgtgg aaggtgggga agatcctgtg ccggacaggt ttagtttgaa aagaaataag
     8581 cagaagtagg gcaacctact ttttattctg tataaatata ggtttttatg ttgcctttag
     8641 tactctctca aaaggtttgg gagcctttga aggctggaga tagggcgaag caagttcgcc
     8701 tcaatattaa cagtccactg gactgttaaa gccaatcaat cactgcgctc agatgttgac
     8761 acgaactctg cgaagctagg ttggtctttt tgcccagccc ccatgatttt ctatgaaaat
     8821 ttgaaaaaaa tagggcaaaa ctcttgattt tcagccttct ttaagctaca atagtatcac
     8881 gatttcattg atttaactca aacctgttgt gagttaagtg aatgaatcat cattcaccat
     8941 gctgtttgct gagcttgact ccgggcagtg tggctatttt tttgtctttc ttgccatagg
     9001 agacagtcga gtctttttac tcaatgaaaa tcaaaaaaag aaactagata acaagcaaca
     9061 gaccgtactg gtatacggta aaatgcatct gacatggttt ggatttgatt ttcgaagggt
     9121 attcgtgttt agtgaaatgt caccagaggg gtccttatga atattgaaac attagagtat
     9181 atagaagaaa cagccaaaaa ccacattgtc ttatttgaac cacagattcc acagaataca
     9241 ggcaatattg ctcgaacttg tgctgcaacc aatagtcctc tccatatcat taagcctatg
     9301 ggatttccaa ttgatgaccg taagatgaaa cgtgcaggac tagattattg ggataagtta
     9361 gatgttcgct tctatgacaa tttagcagaa tttatggatt atgccagcaa acaaggacgt
     9421 gttcacttag tatctaaatt tgctgataaa acctattcgg atgaagacta taatgatggg
     9481 aacattcatt atttcttatt tggtcgtgaa gataagggct tgccagaaga ttttatgcgt
     9541 gaacatccag agaaggctat ccgtattcca atgaatgatg aacatgtgcg tagtcttaat
     9601 gtctctaata cagcgtgtat gattgtatat gaagctcttc ggcagcaaca gttcgctggt
     9661 ctagatttgg ttcacaccta tgatggggac aagttaaaat agtgaaaact taccaagctc
     9721 ttgcaaatgc aaggcttttt tggtatgatg aaatagtctt cagggcaggg tgaaattccc
     9781 gaccggcagt gtaaaacgtg gtaatttgtt ttaagactag gcagcgaatc gaagaccata
     9841 ctgaatgtat gtcgagatga gctaacgcag tattagaaca aaatagaccg ttggtagtct
     9901 gcgagcgaaa gctgatgtgg tgcaattcca caaccgacag taaagtctgg atgggagaag
     9961 acgaagtgga ggctttttgc ctgctcttcc acgcttcttc aattttcaaa aattggagga
    10021 accttatgac aaacacacgc aaaatgacga ttatcgccat tctctcagca gtatcctttc
    10081 tgctcatgta tttgaaattt ccgctgattc caacagccag tttcctggaa gtggattttt
    10141 ctctcgtacc gattttattt ggtctgcttg ttctagatat gaagtctagt tttgctatcc
    10201 ttttggtacg aacttttctc aaactgattt taaataatca gggaccatcg acaattattg
    10261 gcttaccgat gaatattgca gctatgtcag ttttcatttt agcagtagct tatttctgga
    10321 aaaatgaaca aacgttaaaa aattatttaa aagcagccgc tgttgcgaca attggttcga
    10381 cagcagtgat gctggtatta aattacgttt atgcggtgcc tgtttatgca gcctttgcaa
    10441 attttgatat taaagaaatc ttggggctga ctaactatct tttgatgatg gtacttcctt
    10501 ttaatctttt acaagggatt gtgctttcag caatatttta tatctgttac aaagcagctc
    10561 aacctatttt aaagaaagtt taacctttat aaggtaaact atactgtatg aagaataaac
    10621 aaatccattt acgaaacgca tctttttttg ctctggcctt tgtgatattg ggctatgtag
    10681 taaaatttta tcctagtacc ctagcaggtt ttgactcgac tgtccagtct gccattcgtg
    10741 gaaatttacc aagtggagcc agtcaatttt ggacctctat tactgtttta ggaaatacgg
    10801 ttattatttt agctattacc ttgattctag ctgctatctt ttactactat aaaaaatgga
    10861 agatagaagc ttatttccta cttgctagtt ttgcggcgat gggtgtggca tccacagccc
    10921 tgaaatatgt ctaccaacgt ccgcgtccaa gtattgaatg gttgattgat accatcggtt
    10981 attcatttcc tagctggcat actgcatcaa ctatgatgat tgtaggagca gttgttatta
    11041 ttgttcacca gaggatgaag aaaggcatag ctagacttgt gctaaaagcc gtgttgattc
    11101 tacttgctgt cctggtagct gtatcccgta tctatattgg ggttcactac ccgacagata
    11161 ttatcggtgg ttggctactt gccgtcactc ttttacaaat gatgtatcca ttttacgatc
    11221 agaaacgctt tgaatggcgt ttccaaagta agcaaaaata gtcaatttga caccaactcc
    11281 tactgtaaaa ggtaggagtt ttaacttgtc atctttctat tatcgttact tcgctttgcc
    11341 gtcctttgta gaagtggctt atttcacaag atttattgag gctgagctca ggcacaacgc
    11401 catttcttag agttcgcgct ggacttatag gaaaatagtg caaaaatcct ccgagaaatt
    11461 ctcagaggat aatgatgtat aaatctagtt tttgattgga ccatcagcaa cggcttcaac
    11521 agcatttttt atcgtttggg cgaagacttc gcctccatta ctttcaagat tgaaatgaac
    11581 aaggtcggta ttgtaccaaa ttggtggatt atcctttgca acttgatacc agtctgcaat
    11641 ggaaatgtaa tcatattttt cagccaattc tttctcgtat tgaccagttt gataggaaat
    11701 ggattcgctc ggagtgaagt ttccatcgta aggagtgaca aaaataaggc gacgtccttt
    11761 aggaaaatct ttgactagct ggtctagtag ttcctgataa ttatccgtag tatttgtacc
    11821 tagggctaca actacagtct ctttcaagct attattttgc ttataaagat ttatcaattt
    11881 agcaatttca gagagatttc gactgacggt tccatcgatc tgtgcatctg gtagcgcggt
    11941 ctgtatggca gaactagcgc gaacagtaac agaatcgcca aagatgataa ctcctttttg
    12001 aatttcaaaa ctagtagctt tagcgttttc agcagcagat cgtgttgtat ttagttgggt
    12061 ctgagcttga taaagattgc taaccatgct ctcttgttca aaacttccta attttggtgc
    12121 aaacagacag atacctaagc caactatggt caagcaagca aagctaccaa gaagccattt
    12181 tttatatggt ttcaaatcta tgggtaatcc aaacaagctt cctactttac cagcaatata
    12241 tggttctaga atatagaagg aaatagttga taggatagta gaaaaaacaa aggataatcc
    12301 cgcagcgata gtggcatttg tcagttgtgt aaagataatg agcaagggcc aatggaagag
    12361 gtagatacca taagaaatac ttgcaaagaa gaggataatt gcaggttctt tgacatcttc
    12421 ggttttctcg tgaagtaccc gagcagcaaa aatcatagtt gctgtagcta aactggatag
    12481 aagaaaacca attagatagg tccagatgga actgaattga agagtaaaga gaagaagaag
    12541 ttctactcct agtcctccaa gaaacagacc tatagcttgt ttgatactca atgtattcac
    12601 ttgttgctgg aaggcagttg ttgtatttcc gataccagcg atggttgcga gaacgcttcc
    12661 gaggaagaat gggaaggtat gtgtgaagct cgagtagtaa atggttgaga aattatccac
    12721 aaagaaactt gagatagaca ttgctaaaaa acttagcgag aagaggctta tagatgttag
    12781 aaaaattgat ccacgtagtt gccccacact ttttgagatg cgagtcagat accaaactaa
    12841 accagcccag acaaggtaat actgaacttc tatggctagt gtccaagtat gcacaaatag
    12901 atgaggtgaa aattgatttt cgtaactacc acctgataga atttcaaaga agtttgtcat
    12961 gaatccgagc gcagcggcta tctgattgcc gatattggca atgaaatcat tgcggacaag
    13021 cagagcaagt ggaaggacca aaagaatcgt taaaaccaga ggggggagaa tccgatagaa
    13081 ccttcttctg aaaaagctga caatatctat ttttttatgt tttgcaaact catcaatcaa
    13141 aagggcggtt gtcaaatatc cagacagggt aaagaagagg tcaaccccaa caaagccacc
    13201 tgggaaatat ttgataaaga aatgatagag taggacaaag actaatccaa tcacacgtat
    13261 aacggataac catttaattt tcatattgat aaatcccctg tttaaaagtt ccttcgtagg
    13321 tagcttgtcc ttcggtggtc aattttcctt ttccatcggc gtagccgttt ttaaattgcc
    13381 cggtataaac ccacccactt gcagatgtgt aggttccttg accgtggaaa attccatttt
    13441 taaattgccc ctcgtagaca tctccattat caaaggtcaa ttttccttga ccattcattt
    13501 tatttgcgac cacataaccg ttgtactgca gggcgccatt attgaaagtc atggcttgct
    13561 tgctggcaat tcgccctgta aatacagaga gggcacagat gaggatgata ataagggaga
    13621 ggatttcaag ttttggacgg gtgaaatgac ttgttatatc atgccatttt atgctaggta
    13681 atttgaactc tttcatcgtt ttactctttc ataggatttg accgatgcta atcagttctt
    13741 tctaggatac tgtaagaaat tatcagttca gtcagaaata gtttaccatt tttttacaaa
    13801 tattacaaaa atattacata gaaaatctta ttcttgatca aatgactgta acctgtccaa
    13861 ccagtcttgg caacctttct ttaccatttc gtaggtttcg tcaaaatctc cagtatacca
    13921 agggtctggt acggattttt ctgcgaattg aaaaatctta ttttgggcat gtgctggtgc
    13981 cattttctgt aaatctcgga cattattgct gtccatacca atgatgacat caaattcctc
    14041 aaagtctgct tgagagattt gttgagaggt tttggtcttg tcatagggaa tcttgtgctt
    14101 ttggaaaatt ttctgtgttc cagagtagat aggattacca tgttcccagt ttgaagtggc
    14161 acggctttca atgtgaaatt gacttgtcat gtctttcatg acaaattcag ccatagggct
    14221 acgacaaata tttccaagac agacaaaaac gatttttttc atagattctc ctttcgtggc
    14281 ataagaaaag agaaggcaga ctcgacaggt aaatgtctga gttcccctct cttgcatatt
    14341 tttgttcggg ctataggtaa aagaccaaat taagtgtaat gttcattatc aaacagtgca
    14401 cgaccaatcc tacctatatt gaactctttg tttatttctc ttctttgtcg aaagcttttt
    14461 tgatgccgtt aatggcacct tcaagcccgt cttttgcatc ttcgactagc tcttttgctt
    14521 tgccaattcc tttctcaaca agtccttcaa cttcgatttc cttgtcgccg gtcagcttac
    14581 caattacctc ttttgcagag ccagatagct gttctagttt tgcgtcaaat ttttcttctg
    14641 acataaaatc cctcctcgat atacacatat gggggcgatg attttctaaa aagaaaatca
    14701 catactattc ttacttacta gtctaacgta tataagttaa tatagcaagg gatttgtctg
    14761 tcaataagcc agaggaggat gagggataat tcttgcaatt ttttctt
//
LOCUS       DASFSW010000043        14564 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.043, whole genome shotgun sequence.
ACCESSION   DASFSW010000043 DASFSW010000000
VERSION     DASFSW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.043"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            32..679
                     /locus_tag="U1099_000958"
     CDS             32..679
                     /locus_tag="U1099_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM4867290.1"
                     /translation="MTVGKTVPLDWFEKAQGKKLLGLACGGGQQGPIFAAHGYETTIL
                     DFSKEQLDKDRLVAERENLDLQTVQADMTQAFLFEDESFDIIFCPVSNVYVEDLTNMW
                     QESYRVLKKGGLLMVGYMNPWIYVFDSDDVWDYPDKTLVPKYSLPFNARQLEEAGQIT
                     IDPEYGYEFSHTLEEQIAGQLRAGFAMIDFYESKDSRNRLTQYASDYLANLSIKW"
     gene            842..2047
                     /gene="sstT"
                     /locus_tag="U1099_000959"
     CDS             842..2047
                     /gene="sstT"
                     /locus_tag="U1099_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEM4867291.1"
                     /translation="MNKIISVWKKMNLIRKIGIGVVLGVLLGLIAPKITVIALFGSLF
                     VGALKAIAPLLVLTLVAHALSQAPAGQKSNMRTVICLYLFGTFAAAFIAVGASYLFPI
                     KLVLSTTTTTDIIPPQGIAEVFQDLLLKVVDNPINALATANYIGVLTWAAVFGLAFRH
                     ASKTTKDLLQSAAEVTSKVVGWIIGLAPFGIMGLVFDTIANNGLTALKDYGLLLLLLV
                     GSMIFVALVVNPLIAFLVMKKNPYPLVFECIRVSGVTAFFTRSSAANIPVNMQLCKRL
                     GVDPDTYSVSIPLGATINMAGAAITINILTMAAVHTLGISVDFSSALLLSVVASLSAA
                     GASGVAGGSLLLIPVACSLFGIPNELAMQVVGVGFVVGVIQDSCETALNSSTDVLFTV
                     VAERSAWKK"
     gene            2309..3709
                     /gene="pepV"
                     /locus_tag="U1099_000960"
     CDS             2309..3709
                     /gene="pepV"
                     /locus_tag="U1099_000960"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEM4867292.1"
                     /translation="MTVDFRAEFDKRKDEFLADLFDLLRINSERDDSQADAQHPFGPG
                     PVRALDKFLEIAQRDGYPTKNVDNYAGHFEFGEGDEVLGIFGHLDVVPAGSGWDTDPY
                     EPQIIDGRLFARGSSDDKGPTMACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADMD
                     YYFENVGLPLPDFGFSPDAEFPIINGEKGNITAYLHFAGENSGAAKLHSFTGGLRENM
                     VPESATAIISGDLADLDSKLADFTAAYGLKADAETLENGQIQVTVIGKSAHGSTPEEG
                     VNGATYLAKFLSQFAFDGAAKAYLDLAGQVLLEDHDAQKLGVAIYDEQMGALSMNAGV
                     FKFDETSSDNTIALNFRYPKNTNPETIKAGLEKLGVEAVSLSEHGHTPHYCPIDDPMV
                     ATLLSVYEKHTGLKGHEQVIGGGTFGRLLKRGVAYGAMFPGDVNTMHQANEFIEVEQL
                     YRAAAIYAEAIYELIK"
     gene            3750..4208
                     /locus_tag="U1099_000961"
     CDS             3750..4208
                     /locus_tag="U1099_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867293.1"
                     /translation="MKLKELTVADLEMVKQLFLSVFSQEPWNDDWSDEEQLDCYIGDL
                     LGHPRALCFGLFDPEKVIGLSLGYIRYWCEGTEYRIEEFCISRDYQGQGHGASLLADI
                     EKQLADRKIVHILLQTERNLSAYSFYKKCGFRALEEDVTMVKKVGHHGTC"
     gene            4195..4773
                     /locus_tag="U1099_000962"
     CDS             4195..4773
                     /locus_tag="U1099_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEM4867294.1"
                     /translation="MELADIRQAIIEDVANRDFTNKGIAPLFQAPSTAKILIIGQAPG
                     LKTQEKGRLFDDASGNNLRQWLGVDRQTFYESGHFAILPMDFYYPGKGKSGDLPPRKD
                     FAPKWHPLIMKQLPQIELTILVGNYAQTYYLEKSQRNLTERVKHADDYLPKYFPLPHP
                     SPRNNIWQAKHPWFVEQTLPILKNNINILLGK"
     gene            4845..5627
                     /locus_tag="U1099_000963"
     CDS             4845..5627
                     /locus_tag="U1099_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEM4867295.1"
                     /translation="MRKWKKIIIGCFAILTVCLLFAPTLARANQIPDRPTGTTVVDET
                     QLLSSETIAEIDQLNRSWASTEQALQVGVYVTESLSSDIESLANETFRHWQVGFAGTD
                     NGVLLVVAIADRAFRIETSDNAATVITDVEAKDILENAREFFRQEDYSGGISYIVNSI
                     GDRFYGTSIGKNQLAAIEEGTSEKDDGFWLFLIVILIAILFGIIDKSSRGGGGPGNLL
                     WMLVDDHHHYHNHHSSNSSSSSFGGGSWSGGGGGGGGASSGW"
     gene            5700..6281
                     /locus_tag="U1099_000964"
     CDS             5700..6281
                     /locus_tag="U1099_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEM4867296.1"
                     /translation="MKKKWLAILLPILALLFLGMGAVGQYNGLVDSYAEVENAQANVD
                     TQLQRRYDLIPNVVAAVKGAMEHEEEIFTAIAEARAKIGSSQVGTSDYNQAQSQLDSA
                     VSRLLVVAENYPQLTANQQVSALITELEGTENRILVARKDYNSVATTYNKKIKRFPTS
                     IYANLFGYEKVELFQATNDAATTVPSVDLSDKE"
     gene            6293..7756
                     /locus_tag="U1099_000965"
     CDS             6293..7756
                     /locus_tag="U1099_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM4867297.1"
                     /translation="MDFGFDILAIMIPIIVVVLFVLLFVFGYVSAKPNEAIVITGLGK
                     PRTLIGRSGFMIPFIEKRSYISIEQFSTDVQTTDFVPTLDFINVKADAVVKVKVGISD
                     ELLNAAAQNFLNWKTADISASIQDVLEGNLREIIGQMELRDMVNNRQAFAEKVQSNAA
                     PDLAKMGLEIIAFTVQSFTDDNDVIKNLGIDNIVTIQKDAANARAKAEREQAEVRARE
                     DKAANDARVAADLEIAKKQNELAIEQANLKRQSDVQLAQANAAYGIEEQAQRKEIERA
                     TAEANIVKQQKEAEVKAEEVKVREQELSATIRKQAEAEKYARQQAAEADLIERQRKAE
                     AELYETQREAEAQKARAEAAKYAAEQEAAGIEAKGRAEAEAIRLKLEAEAEGLSKKAD
                     AMAKFNDAAVTEMVVNVLPEIAKNIAAPLGNVDKITMYGEGNASKMVGDLMTTMDKTT
                     EGLGLNVRDLISATLTGRAMSTGLLTAQETKEAEGDK"
     gene            7845..8153
                     /locus_tag="U1099_000966"
     CDS             7845..8153
                     /locus_tag="U1099_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867298.1"
                     /translation="MKMFRNSKKSKLFIQKINELLSDSELKLSKALKFQLLEAMELCE
                     KGSKISYLSYKIYPWVLEELALNRIQSDKLKMFKRYLEQERWKYYFGSALGMAFTSIR
                     "
     gene            complement(8275..9350)
                     /locus_tag="U1099_000967"
                     /pseudo
     CDS             complement(8275..9350)
                     /locus_tag="U1099_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9462..10835)
                     /gene="mnmE"
                     /locus_tag="U1099_000968"
     CDS             complement(9462..10835)
                     /gene="mnmE"
                     /locus_tag="U1099_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEM4867299.1"
                     /translation="MITKEFDTITAISTPLGEGAIGIVRLSGTDAFAIASKVFKGKDL
                     ATVPSHSLNYGHIIDPATGQVLDEVILGVMRSPKTFTREDVIEINTHGGIAVTNEILQ
                     LLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDVIRAKTDKAMHNAVRQLDGSLSQ
                     LINDTRQEILNTLAQVEVNIDYPEYDDVEEATTELVREKTLQFQALLENLLRTARRGK
                     ILREGIATAIIGRPNVGKSSLLNNLLREEKAIVTDIAGTTRDVIEEYVNIKGVPLKLI
                     DTAGIRETDDIVEKIGVERSKKALEEADLILLVLNASEPLTEQDRNLLAISDLANRIV
                     LLNKTDLEEQIEADQLPEDVIRISVLKNQNIDQIEEKINQLFFENAGLVEQDATYLSN
                     SRHISLIEQAVQSLQAVNDGLEMGMPVDLLQVDLTRCWQILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            11519..>14564
                     /gene="cas9"
                     /locus_tag="U1099_000969"
     CDS             11519..>14564
                     /gene="cas9"
                     /locus_tag="U1099_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637067.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEM4867300.1"
                     /translation="MSNGKILGLDIGIASVGVGVIDAQTGEIIHASSRIFPSANAANN
                     AERRTFRGSRRLIRRKKHRIKRLDDLFNDFHINLDGEMSTDNPYVLRVKGLSQKLTVE
                     ELYISIKNIMKRRGISYLDDAESDNEAGRSDYAKAIERNRQLLTSKTPGEIQLERLEK
                     YGQLRGNFTIIDEEGQSQQIINVFSTSDYVKEVEKILDCQKMYHKFISDEFCDKLIEL
                     LREKRKYYVGPGNEKSRTDYGIYRTDGTTLENLFGILIGKCTFYPDQYRSSRASYTAQ
                     EFNFLNDLNNLTVPTETKKLSQEQKEFLVNYAKETSVLGAGKILQQIAKLADCKVEDI
                     RGYRLDNKDKPELHTFETYRAMKGLVPLVDIGVLSREQLDILADILTLNTDFEGIREA
                     LKKQLPNVFDEKQVKGLASFRKSKSQLFAKGWHNLSQKIMLEVIPELYATSDEQMTIL
                     TRLGKFEKSSVAEYPSSINVDEITDEIYNPVVAKSIRQTIKIINASIKKWGEFDQIVI
                     EMPRDRNEDEEKKRIADGQKANAKEKADSILRAAELYCAGKVLPDYVYNGHNQLATKI
                     RLWYQQGERCIYTGQPISIHDLIHNQNQYEIDHILPLSLTFDDSLSNKVLVLATANQE
                     KAQRTPYNYLKSATSAWSYREFKDYVTKRKGIGKKKCEYLTFEEDINGFEVRSKFIQR
                     NLVDTRYASKVILNALQDYFKISGIQTKVSVVRGQFTSQLRHKWGIEKTRETYHHHAV
                     DALIIAASSQLRLWKKQESPLVVDYQEGRQVDLETGEILELTDEQYKELVYQPPYQGF
                     VNTISSSAFDNEILFSYQVDSKVNRKISDATIYATRNAQLGKDKTEGIYVLGKIKDIY
                     TQAGYEAFLKRYTKDKTSFLMYHKDLDTWEKVIEIILRDYREYDEKGKEIGNPFERYR
                     RENGYVKKYSRKGNGTAIKSLKYYDNKLGNHIDITPENSRNAVVLQSLKPWRTDVYFN
                     KETGKYEFLGIKYSDLSFEKGTGEYGISQEKYDSIKIAEGVAK"
BASE COUNT         4368 a         2711 c         3376 g         4109 t
ORIGIN      
        1 accagtcgag ttctttccca ctcgcgtttc tttgacggtt ggaaagacag taccgcttga
       61 ctggtttgaa aaagcccaag gtaagaaatt gctgggtcta gcatgcggtg gtggtcaaca
      121 gggaccaatt tttgcggctc atggctatga gacaactatt ttggacttct ccaaggaaca
      181 gttggacaag gataggttag ttgctgagcg ggaaaatctg gatttgcaaa cggtccaggc
      241 agatatgaca caagcctttc tttttgaaga cgagagtttt gatatcatct tttgtcctgt
      301 ttcaaatgtt tatgtcgaag atttgaccaa tatgtggcag gagtcttatc gggtcttgaa
      361 aaaaggaggc ttgctcatgg ttggctacat gaacccttgg atttatgtat ttgacagtga
      421 cgatgtttgg gattatccag acaagacctt ggttccaaag tatagcctac ctttcaacgc
      481 acgacaatta gaagaagcag gacaaatcac gattgatcca gaatatggct atgaatttag
      541 ccacacctta gaagaacaga ttgctgggca attacgagct ggttttgcca tgattgattt
      601 ttatgaatcc aaagacagtc gcaatcgctt gactcaatat gcctcagatt acctagcgaa
      661 tttatcgata aagtggtaga aacaagccac cgaaagatgg tcttttcaac tcagtaggta
      721 aagttgaaat gattgtgcaa gatttttcag gcttccgctg tataatatct cattttttat
      781 gctattgacc tgactggatt tttctgctat gattgaaaaa tgtttgtttg gaggtaaatg
      841 agtgaataaa ataatatctg tatggaaaaa aatgaattta atccgaaaaa tcggaattgg
      901 tgttgttttg ggagtgttac taggcttaat tgctccaaaa ataacggtca ttgctctctt
      961 tggtagtcta tttgtaggag cattgaaagc tatagcccct cttttggttc tgacactagt
     1021 tgcacatgct ctatcacaag ctcctgctgg tcaaaaatcg aatatgagga cagttatctg
     1081 cttgtatcta tttggcactt ttgcggcggc cttcattgct gtaggagcaa gctatttatt
     1141 ccctattaaa ctggttcttt ctactacaac gactacggac attattcctc cacaagggat
     1201 tgctgaagta ttccaagacc tcttgttgaa ggtcgttgac aatccaatca atgctcttgc
     1261 aacggctaat tatattggtg tgttgacatg ggcggcagtg tttggcttgg cctttcgtca
     1321 tgcaagcaag acaaccaaag atttattgca atctgcggca gaagtgactt ctaaagttgt
     1381 aggctggatt attggtctgg caccatttgg tatcatgggc ttggttttcg ataccattgc
     1441 aaataatggt ctgacagctt taaaagacta tggtttactg ctcttactct tggttggaag
     1501 catgattttt gtcgcgcttg ttgtcaatcc cttgattgct tttcttgtaa tgaagaaaaa
     1561 tccttacccg ctggtatttg aatgtattcg ggttagtggg gttacggcat tttttacaag
     1621 aagttcagca gctaatatcc ctgtcaatat gcagctctgc aaacgtttag gagtggatcc
     1681 cgatacatac tcagtatcca ttccgctcgg tgccacaatt aatatggctg gcgcggctat
     1741 tacgattaat attttaacaa tggcagctgt tcatacactt ggcatcagtg ttgatttcag
     1801 ctcagctctt cttttatccg ttgttgcatc actatcagca gcaggagcat ctggggtagc
     1861 aggtggctcg cttctcctca tcccagtagc atgtagtctt tttggtattc caaatgagtt
     1921 agccatgcaa gtagttggag tcggatttgt ggtcggagtg attcaggact cttgtgaaac
     1981 agccctcaac tcatcaaccg atgttttgtt tacagttgta gcagaacgat ctgcttggaa
     2041 aaaatagaaa gaagttggga gaccaacttt ttttcttcgc tttacttctg aaatctcttt
     2101 cattttgtga taaaatagac ttactttgat atagtctttg ggtttacttt tagaactagg
     2161 caacgagccg caggcataac tggagttagg caaggtgagt tcaaacactc cagtggagtg
     2221 tttgaagttg gaaataagga aacgatgttt cctcgctcgt cgaagtaata aaagataaac
     2281 caaatgacga cactgaaaag gagaacatat gacagtagat tttagagcag agtttgacaa
     2341 acgcaaagat gagtttctag cggacctctt tgacctctta cgcatcaatt ctgagcgtga
     2401 cgacagccag gcagatgccc agcacccatt tgggccagga ccagtgcgtg ccttggacaa
     2461 gtttctagaa attgctcagc gtgatggcta tccgaccaag aacgttgaca actacgcagg
     2521 tcactttgag tttggtgaag gcgacgaagt ccttggtatt ttcggtcact tggatgtggt
     2581 gccagcagga agcggatggg ataccgaccc ctacgagccg caaatcatcg acggtaggct
     2641 ctttgcccgc ggctcctctg acgacaaggg gccaaccatg gcttgttact acggcttgaa
     2701 aatcatcaag gagttgggcc ttccgacctc taaaaaagtc cgtttcatcg tcggtaccga
     2761 tgaagagtca ggctgggcgg acatggatta ctactttgag aacgttggtc tgccattacc
     2821 agatttcgga ttctctccag acgctgagtt cccgattatc aacggcgaaa agggaaatat
     2881 cacagcttat cttcattttg cgggggaaaa tagcggagct gctaaactgc attccttcac
     2941 aggcggcctg cgtgagaaca tggtgcctga atctgcgaca gctattatct ctggcgacct
     3001 agcagacctg gacagcaagt tggcagattt cacagcagcc tatggcctaa aagctgacgc
     3061 ggaaaccctt gaaaacggtc aaattcaagt caccgtcatc ggaaaatcag cccacggttc
     3121 aaccccagaa gaaggtgtca acggagcaac ctatttggca aaattcctta gccaatttgc
     3181 ctttgatgga gcagccaaag cctatcttga cttagcagga caagtccttc tggaagacca
     3241 cgatgcccaa aaactcggcg tagccatcta cgatgagcaa atgggagctc tttctatgaa
     3301 tgcaggtgtc tttaagtttg atgaaacctc atctgacaac acaattgccc tcaacttccg
     3361 ttatccaaaa aataccaacc ctgaaaccat caaggctggt ttggaaaaac tgggcgtgga
     3421 agctgttagc ctgtctgagc atggccatac cccacattac tgcccaatcg atgacccaat
     3481 ggtcgcaacc ctcttgtctg tttatgaaaa acacacaggc ttgaaaggtc acgaacaagt
     3541 aatcggtggc ggaacatttg gacgcctgct caaacgtggc gttgcctacg gagccatgtt
     3601 cccaggcgat gtcaacacca tgcaccaagc caacgaattt atcgaagtcg agcaactcta
     3661 ccgcgccgct gccatttacg cagaagctat ctacgaactc attaagtaat acatgaagac
     3721 gggtacgccc gtctttttta ggaggaagaa tgaaactgaa agaactcacc gttgcagatt
     3781 tggaaatggt caaacagctc ttcttgtctg tctttagcca agaaccttgg aatgatgatt
     3841 ggtcagatga ggaacaatta gattgctaca taggcgattt actgggacac ccaagagcac
     3901 tctgttttgg cctgtttgac ccggaaaaag tgatagggct gtcacttggt tacattcgct
     3961 attggtgtga gggaacagag tatcgaattg aagagttctg catttcccgt gattaccaag
     4021 ggcaaggaca tggtgctagt ttattagctg atattgagaa gcagttagct gatagaaaaa
     4081 ttgttcatat tcttctgcaa accgaacgca acctttcagc ctattctttc tacaaaaaat
     4141 gcggtttccg agccttagaa gaagatgtga ccatggtcaa gaaagtagga catcatggaa
     4201 cttgctgata ttcgtcaagc catcattgag gatgttgcta accgagactt tacgaacaag
     4261 ggaatagctc cccttttcca agctccttcg accgcaaaaa ttctcattat tggacaggca
     4321 cctggcttga aaacccagga aaaagggcgt ttatttgacg acgccagtgg taacaacctt
     4381 cgtcaatggt tgggcgtgga tcgacagact ttttatgaat cgggtcattt tgctattctt
     4441 cctatggatt tctattatcc aggaaaaggg aagtctgggg atttgccgcc tcgcaaggat
     4501 tttgcgccca aatggcaccc actcattatg aagcaactcc cccagattga gctgaccata
     4561 ttggtaggca attatgctca aacttattat ttggaaaaat cccaaaggaa cctgacggag
     4621 agagtgaaac atgcagatga ttaccttccc aagtattttc ctttgcccca tccatcacct
     4681 agaaacaata tctggcaagc taaacatcct tggtttgttg aacagactct accgatactg
     4741 aaaaacaaca taaatatatt gttgggaaaa tgataatatg atataattgt atatatatac
     4801 ctaataaatt tttcttatct ttgttcatcg aaggaggtgt tctgatgaga aaatggaaaa
     4861 aaattatcat cggctgtttc gctattctaa cggtttgctt gctatttgca cccacactag
     4921 ctagagcaaa tcagatccca gaccgaccca caggtacaac ggtagtggat gagacgcagt
     4981 tactctcgag tgagaccatt gcagaaattg atcaactcaa tcgaagttgg gcaagcaccg
     5041 agcaagcttt acaggttggt gtttatgtaa cggaaagtct gtctagtgat attgaaagtt
     5101 tggctaatga aacctttcgt cactggcagg tcggttttgc aggaacggac aacggagttc
     5161 ttttagtggt tgccatagct gacagagcat ttcggataga aacctcagac aatgctgcca
     5221 cagtcatcac ggatgtagaa gccaaggata ttctagagaa tgcaagggaa ttctttcgac
     5281 aggaagacta cagtggagga atttcctata tcgtcaactc cattggggat aggttttatg
     5341 gtacaagcat tggtaaaaat cagttagctg ctatagaaga aggtacaagt gagaaggatg
     5401 acggcttctg gctgtttctc atagttattc tgatcgctat cctatttggt attattgaca
     5461 aatctagtcg tggtggtgga ggtccaggca atctactctg gatgttggtt gatgatcatc
     5521 atcactatca caatcatcac tcatctaatt cctcatcgtc tagttttggc ggaggtagtt
     5581 ggtcaggtgg tggaggcggc ggtggcggag cctcttctgg atggtaaggc atcgtataag
     5641 gcttctgata gttgtttgct tttttacgaa tgtttaatcg tagttagaaa ggaaatctta
     5701 tgaaaaagaa atggttagcc atacttctcc cgattctcgc tctgctgttt ttaggaatgg
     5761 gagcagttgg gcaatacaat ggcttagttg acagttatgc tgaggttgaa aatgcgcaag
     5821 ccaatgtaga cacacagttg caacgtcgct atgacttgat accaaatgta gtcgcagctg
     5881 tcaaaggcgc catggaacac gaggaagaaa tatttactgc tattgcagaa gcgcgtgcta
     5941 aaatcggttc cagccaagta ggaaccagcg actacaatca ggcacaaagt cagctagatt
     6001 cggcagtgtc tagattgttg gtagtggctg agaattatcc gcaattaaca gccaaccaac
     6061 aggtatctgc cctgataaca gagcttgaag gtacggaaaa tcggatactg gtagcccgta
     6121 aagactataa ttcggtagca actacctaca ataaaaaaat caagcggttc ccgacctcca
     6181 tctatgctaa cttatttggc tatgaaaaag tcgagctttt ccaggcaaca aatgatgcgg
     6241 cgacgactgt accaagtgtt gatttgagtg ataaggaata aaggagataa atatggactt
     6301 tggatttgat atattggcaa ttatgatacc aattattgtt gttgtactat ttgttttatt
     6361 gtttgtattt ggttatgtgt ctgctaagcc taacgaagcg attgtcatta ctggcttggg
     6421 caaacctcgg actttaattg gtcgttctgg ttttatgatt ccctttatcg aaaaacgctc
     6481 ctatatctct attgagcagt tttcaacaga tgtgcagacc acagattttg taccgactct
     6541 tgattttatt aacgtaaaag ctgacgcggt cgtaaaagta aaggttggga tatccgatga
     6601 attgctcaat gcggcagctc aaaacttcct taactggaaa actgcagata tttctgcctc
     6661 tattcaagat gtcttggaag gaaatctgcg tgaaatcatt ggccagatgg aactgcgtga
     6721 catggttaat aaccgccagg cttttgcaga aaaagtccaa tccaacgcag ctcctgactt
     6781 ggctaaaatg gggctggaaa tcattgcctt tacggttcaa tccttcacag atgacaacga
     6841 tgtcattaaa aacctgggta ttgacaatat tgtgaccatc cagaaggatg ccgcaaatgc
     6901 tcgtgccaaa gcagagcggg aacaagcaga agtccgtgcc cgtgaggata aggcggctaa
     6961 tgatgcgcgt gttgctgcag acttagaaat cgccaagaaa cagaatgaac tagctattga
     7021 gcaagctaat ctaaaacgtc agtccgatgt ccaactggca caggcaaacg cagcctatgg
     7081 cattgaggag caagctcaac ggaaagaaat cgagcgtgct actgctgaag ccaatattgt
     7141 caagcaacaa aaagaagcag aagtaaaagc agaggaagtt aaggtccgtg aacaagagct
     7201 ctcagctact atccgcaaac aggcagaggc agaaaaatac gctcgtcaac aagctgcaga
     7261 agcagatttg attgaacgtc aacgcaaggc agaagcagaa ctctacgaaa cgcagcgtga
     7321 agcggaagct caaaaagcgc gtgccgaagc tgctaagtat gctgcggagc aagaagcggc
     7381 aggtattgag gccaaaggac gtgccgaagc agaagccatc cgtttgaaac tcgaagcaga
     7441 ggcagaaggt cttagcaaga aggcggatgc catggctaag tttaatgatg cagcggtgac
     7501 agaaatggtg gttaatgttc ttccagaaat cgccaagaat attgctgcac cacttggaaa
     7561 tgttgataag attaccatgt acggcgaagg gaatgcctca aaaatggtgg gagacctgat
     7621 gacgaccatg gacaagacaa cagaaggtct aggactcaat gtccgtgatt taatcagcgc
     7681 aaccctaact ggtcgtgcca tgtcaacagg tttactgact gcccaagaaa caaaggaagc
     7741 ggaaggggac aaataaaaat aataagcagt tggaagagat gggtgactga gtaggtttac
     7801 accatcattt ccatcgcatg attgtttcag aaatccctac gaagatgaaa atgtttagaa
     7861 acagtaaaaa aagtaagttg tttatacaaa aaataaatga actattatct gattcagaat
     7921 taaagttgag caaagcattg aaattccagt tgttggaagc aatggagttg tgtgaaaaag
     7981 gtagtaagat tagctatctt tcatataaaa tttatccatg ggtattggaa gaactagctt
     8041 tgaataggat tcaatcggat aaattaaaga tgtttaagcg ctatttagag caagaaaggt
     8101 ggaaatatta ttttggttct gctttgggaa tggcgtttac ttcgattaga taatggttcc
     8161 gaggatttgt aagtacattg attgtgctgt gcaacttcga tagaatatgc gaaagtccca
     8221 agaaagagta atcttgggac ttttacaatc tttattgaga ttcgtttttg tttatcagtc
     8281 atttacccac actccgctga cataattctc agatagaatg gtttgactta aaactttttt
     8341 tgcttgccag tcatagatga gtgccttacc agccagaatc aataataatc tgttaccgtc
     8401 tagttgacgg acatggtcaa tataataagg acgaggatta agctcgtcta gacgatggta
     8461 gctctcttga cctgtttggg tattgtaaat ggtaaaagag attgggctaa acatgtcatt
     8521 ttgatgttcg atgaggagaa gcttctggtc tttgagagca tgtagtgtgc ttggtgaggg
     8581 gtgttctaaa gttactgacg aaaatgtttt tgtttttgta tcaaaggtta ggagttcgga
     8641 tgactgttca atgtcagtat actcacttgt tttttcccta aatttgggag atagatggtt
     8701 ccattgaccc aaggcccctc tcctgtggca atggtattgt cgtacttgaa tacctcttgt
     8761 tttgaaaaat ctttcttatc aaatacaatc aagacatttt cttcgggtcc ttcttgggta
     8821 aaatctttca caaaaccagt taaatagact gacgtttggt ccaccatgac tggagcattt
     8881 gggatgaagt catcgtttaa aacgtgggtt tgaatggtat caaaattcct gtcaaattgc
     8941 gctagtatgt gatagggccc cgctgtataa aagtcttgtc catctgtacc actgatatag
     9001 ctactatact gacttggctt tcgcaagaca gtgccagtat tgaaatcgat ggatatcaga
     9061 ttgtcgttaa tgaatttttg ctgatcgttg gcaaagacta gataccgact gtctatgaca
     9121 taattttcac cagaaccaaa atacccttct gaattcattt tttgggtatg aagcagctct
     9181 ggttcttgac catttagctg aaaggcttcg accttaccgg gataaatcaa gtaaaaatca
     9241 gcttccgacg ttaatgtgat tgtattgtgc tggtaggcat atagtttcca gccgataaat
     9301 ggaaagacaa gacaggatag aaggacaata caaataagaa gttttttcat gggaatgccg
     9361 cctttcactg tttagtatat ctaaatttgt catcttagac aagaaattat gtgttaatat
     9421 acaaataaag tcatgcaagt tttcttacat gacttttgtt cttattttcc aagacagaat
     9481 tggctaaaga gttgggtaat gagttcatct ggagcagcat cacctgttat ttctcccaaa
     9541 atttgccaac agcgtgtcag gtcaacctgt aacaagtcta ctggcatgcc catttccagc
     9601 ccatcgttga cagcttgtag gctttggact gcctgttcaa tgagggaaat gtggcgagaa
     9661 ttggagaggt aggtggcatc ttgctcgacc agacctgcat tttcaaagaa gagttgattg
     9721 attttttctt cgatttggtc gatattctgg tttttcaaga cggaaatgcg gatgacatcc
     9781 tcaggcagtt ggtctgcttc aatctgttct tccaggtcgg tcttgttgag taggacgata
     9841 cggttggcca ggtctgaaat ggctaggagg ttgcgatctt gctcggtcag gggttcagat
     9901 gcgttgagga ctagcaggat gaggtcggcc tcctcaaggg cttttttaga gcgctccaca
     9961 ccgatttttt caacgatgtc gtctgtttca cggatacctg ctgtatcaat gagcttgagg
    10021 gggacgcctt tgatgttgac gtattcttcg ataacgtcgc gggttgttcc tgcgatgtct
    10081 gtaacgatgg ctttttcctc gcggaggagg ttattgagca ggctggattt tcccacattt
    10141 ggacgaccga taatagcggt tgcgatgcct tcacgcagga tttttccacg gcgggcagta
    10201 cggaggagat tttctagaag agcttggaac tggagagttt tctcgcggac cagctctgtc
    10261 gtcgcctcct cgacgtcgtc atactcaggg tagtcgatgt tgacctcgac ctgtgccagc
    10321 gtgttgagaa tctcctggcg ggtgtcgtta atgagctggg agagggagcc gtcaagctgg
    10381 cggacggcat tgtgcatggc cttgtcggtc ttggcacgga taacatccat gacggcctcg
    10441 gcctgtgtca aatccacacg accgttgaga aaggctcgct tggtaaactc gcccggctca
    10501 gccattcgag caccttggcg aatcaggagc tggagaattt cgttggtaac ggcgatgcct
    10561 ccgtgggtat taatctcaat gacatcttcg cgcgtgaagg tctttgggga acgcatgaca
    10621 cccagtatga cctcgtcaag cacttgccct gtcgcagggt caataatgtg gccgtagttg
    10681 aggctgtggc taggaaccgt tgctaagtcc tttcccttga aaaccttgct ggcaatggca
    10741 aaagcatccg tcccagacag gcggacaatc ccgatagcgc cctcaccgag aggggtggaa
    10801 atggctgtta ttgtgtcgaa ttctttggtg atcataataa tgtgctttct aactagaatc
    10861 tgtatttaca agtaaaatac ttggatataa gagatagttt gttgctaatc aaggcagttt
    10921 tttgggggat gggctcaagc ccagtgccac ttctcagagt tcgtgtcaac atctcagcgc
    10981 agtggttgat tggctttaac agtccagtgg actgttaaag gttggagata ggatttgtga
    11041 agcaaatctc tgcaattcgt gtttcacact ccaaatccga cctctacgac tgttgcgaac
    11101 aagttcgctt catttccaac ctccaacagt cactactctg actgttggag ctgtgcgggg
    11161 gtgggagtaa aatagtccgg tggagtgaaa cagtttaggg aactgtttca gctggtcacc
    11221 ttaaaactag aaagtgacca tagaagctga acctactgta ttggaagtga gggaaactcg
    11281 ttttgagatc aatcgagttc tttctcactc cccaaaaact aacgatgaat agctgataaa
    11341 ctagctttag atggaagtgc tgaactgtga atacaggttc ttaaatctta cttataagtt
    11401 taacatggaa tgaagaaaaa agcacatttt agagatgctg atacaaagat atgtattgaa
    11461 aacgttttga aaaaggtgta aaatagatgt ggaataataa agagaaagta ggttttgtat
    11521 gtcaaacggt aaaattttag gccttgatat tggtattgct tccgtcggag taggcgtcat
    11581 agatgcacaa acgggagaaa tcattcatgc aagctctcgt atttttccct ctgcaaatgc
    11641 tgctaataat gcagaacgcc gaacttttcg tggaagtcgt cgattgatta ggagaaaaaa
    11701 acatagaatt aaaagattag atgatttatt taatgatttt catattaatt tagatggaga
    11761 aatgtcgaca gataatccat atgttcttcg cgtgaaaggc ctctctcaaa aactaacagt
    11821 tgaagaatta tatatttcta taaagaatat tatgaagcgc agggggatat cctatctaga
    11881 tgatgcagag tcagataacg aggcgggaag aagtgattat gctaaagcta tcgagcgtaa
    11941 tagacagtta ttgactagta aaacaccagg ggaaattcag ttagagcgct tagagaaata
    12001 cggtcagttg cgtggaaatt tcacgataat tgatgaggag gggcaatctc aacaaattat
    12061 caatgttttt tcaacatcag attatgtcaa ggaagttgag aaaattttag attgtcaaaa
    12121 aatgtatcat aaatttatat ctgatgaatt ttgcgataag ctgatcgaac tattaagaga
    12181 aaaaagaaag tattatgttg gtccagggaa tgagaaatct agaactgact atggtattta
    12241 tagaacagat gggacaactc tagagaattt atttggaatt cttattggta aatgtacatt
    12301 ttatccagat caatatcgtt cttcgagagc gtcctataca gcacaagaat ttaatttttt
    12361 aaatgattta aacaatttaa cagtacctac tgaaacgaaa aagttaagtc aggaacaaaa
    12421 agaattttta gtaaattatg ctaaagaaac gtcggtatta ggggctggaa aaatattgca
    12481 acaaattgcg aaacttgcgg attgtaaggt tgaagatatt agggggtatc gtttagataa
    12541 taaggataaa cccgaactac atacctttga aacatatcga gcaatgaaag gtctagttcc
    12601 gttagtagac attggagtat tgtcgagaga gcagcttgat atattagccg atattctaac
    12661 ccttaatact gattttgaag gaattagaga ggccctaaaa aaacaattgc caaatgtatt
    12721 tgatgaaaaa caggtcaagg ggttagcaag ttttagaaag tcgaaaagcc aattatttgc
    12781 aaaaggctgg cacaatctat ctcaaaaaat tatgctagag gtaattccag aattatatgc
    12841 aacatctgat gaacaaatga caatcttaac acgattagga aagtttgaga agagtagtgt
    12901 tgctgaatat ccttcctcaa ttaatgtaga tgagattaca gatgaaatat ataatccagt
    12961 cgttgctaaa tctatccgtc aaaccattaa aattatcaat gcttcgataa aaaaatgggg
    13021 tgaatttgat caaattgtta tagagatgcc tcgtgatcgg aatgaagatg aagagaagaa
    13081 gcgaattgcg gatggacaga aagcaaatgc aaaagaaaaa gcggattcaa tcctacgtgc
    13141 ggcagaattg tattgtgctg gaaaggtatt gccagattat gtctataatg gacacaatca
    13201 gttagcaaca aaaattcgtc tctggtatca acaaggagag cgttgtattt atactggtca
    13261 gccgatctca attcatgatt taattcataa tcagaatcag tatgaaattg accatatctt
    13321 gccactttct ttgacatttg atgatagttt atcgaataag gttttagttc ttgcaactgc
    13381 aaaccaagag aaagctcaac gtacaccgta taattatctg aaaagtgcta cgtcagcttg
    13441 gtcctatcgt gaatttaagg actatgttac taagcgcaaa ggaattggga agaagaaatg
    13501 tgaatatctg acatttgagg aagatattaa tggatttgag gtgagaagta agttcattca
    13561 gcgtaactta gtggatactc gatacgcctc taaagtcatt ctaaatgcct tacaagatta
    13621 tttcaaaata tcagggatac aaacgaaagt ttctgttgta agaggtcaat ttacatctca
    13681 attgcgtcat aaatggggaa ttgaaaagac acgtgaaacg tatcatcacc atgcggtgga
    13741 tgccttaatt attgcagcat caagtcaatt aagactctgg aaaaagcaag aaagcccgct
    13801 tgttgttgat taccaggaag gacgtcaagt agacttagaa actggagaaa tcttagaatt
    13861 gacagatgag caatataaag aacttgtcta tcaaccgccg tatcaaggat ttgtaaatac
    13921 tatctctagc tcggcttttg ataatgagat actgttttct taccaagtag actccaaggt
    13981 gaatcgtaaa atttctgatg caaccattta tgcaactcgt aacgcacaac ttggaaaaga
    14041 taaaacggaa gggatatatg tccttggaaa aatcaaagat atctatacac aagctggata
    14101 tgaggcattt ttgaaacgtt atacgaagga caaaacttct tttctaatgt atcataaaga
    14161 ccttgatact tgggaaaaag tgattgagat tattttacgt gactatagag aatatgatga
    14221 aaaagggaaa gaaataggta acccatttga aagatatcgt agggaaaatg gttatgtgaa
    14281 aaaatatagc cgtaaaggaa atggtactgc aataaaatca ttaaaatact atgataataa
    14341 gcttggaaat catatcgata ttactccaga aaatagtcga aatgcggtag tattgcagtc
    14401 attaaaacct tggaggacag atgtatattt taataaagaa acaggcaagt atgaattctt
    14461 ggggattaaa tattctgatc tatcgttcga aaaaggtact ggagagtatg gcataagtca
    14521 agaaaagtat gattcaataa aaatagcaga aggagttgca aaaa
//
LOCUS       DASFSW010000044        14267 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.044, whole genome shotgun sequence.
ACCESSION   DASFSW010000044 DASFSW010000000
VERSION     DASFSW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.044"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            109..816
                     /locus_tag="U1099_000970"
     CDS             109..816
                     /locus_tag="U1099_000970"
                     /inference="COORDINATES: protein motif:HMM:NF019322.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM4867301.1"
                     /translation="MRDNSTKSPLYYQLFDSLVEYIQKDLQPHDQLPTEKEIGTDYSV
                     SRTTVRLAMQELEKHGYIYRIQGKGSFVSSLKQENINSFFSLELKNHYDGLETADFES
                     HLVSFTKENGSLALKHLFGLSKQTNLVRIQLEHLLHSELVATETIVAKASYMPELTQE
                     SLEFEGLDSLFQKTGAITKAVDEHYSLARFMPTSSEIDALKITKSIFNQDNELMITST
                     RIINIQKLPYRNFIWTN"
     gene            complement(913..1230)
                     /locus_tag="U1099_000971"
     CDS             complement(913..1230)
                     /locus_tag="U1099_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM4867302.1"
                     /translation="MNIDEKDLLPIMQLIMYSGDAKGHGMRAIQVAKEGCFDEADDLL
                     RQAHDSLIQAHHVQTDMLTREAAGDTLSIGLLMIHSQDHLMNAITFLDLAREMIDLYK
                     KLG"
     gene            complement(1240..1557)
                     /locus_tag="U1099_000972"
     CDS             complement(1240..1557)
                     /locus_tag="U1099_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM4867303.1"
                     /translation="MKRIVLACSAGMSTSLLVTKMQQAAEAQGFEATIYAVSVSELVL
                     EVEKNPADIILLGPQVSYMEEEFKKDPHLANIPIAVINMLDYGTMNGAKVIEYAKSLL
                     DLS"
     gene            complement(1685..1918)
                     /locus_tag="U1099_000973"
     CDS             complement(1685..1918)
                     /locus_tag="U1099_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM4867304.1"
                     /translation="MLTSIIAGAIIGLIAGALTSSDDRRGCLGNIILGLAGSWIGQLL
                     FGDWGPQFAGMALVPSVFGAVLLVAIFSRSRRD"
     gene            complement(1977..3218)
                     /locus_tag="U1099_000974"
     CDS             complement(1977..3218)
                     /locus_tag="U1099_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEM4867305.1"
                     /translation="MVLPNFKENLAKYAKLLVSTGINVQPGHTVQLTIGVEQAELARL
                     IVKEAYAHGAKEVLVNWLDDVIARERLVNVDVELLEQVHPQRITEMNYLLERKASRLV
                     VLSEDPGAYDGVDPEKLSRNTRALSQALNPMRQASQANKISWTLGAASGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRIYEEDPIKAWEEHEARLVAKAKVLNDEQFVKLHYTAP
                     GTDLVLGMPKNHLWEAAGSVNAQGEHFIANMPTEEVFTAPDYRVADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGEIVDVTAEKGDEVMKKLVFDNAGARGLGEVALVPDKSPISQSGV
                     TFFNTLFDENASNHLAIGQAYAFSIEGGTEMIQEELKEAGLNRSDVHVDFMIGSNKMN
                     IDGIREDGTRVPIFRDGEWAI"
     gene            complement(3715..4317)
                     /locus_tag="U1099_000975"
     CDS             complement(3715..4317)
                     /locus_tag="U1099_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM4867306.1"
                     /translation="MSNFAKLQEIRRTIYALGKDLPVSNEEVVALVEKAMKESPSAFN
                     SQSSRAVVLFGAESEAFWNEIAYSELEKVTPAEAFEATKGRLAGFAAGAGTILFFEDQ
                     DVVKGLQESFPLYAENFPIWSEQAHGINLYAVWLAFAEKNIGMNVQHYNPLVDAQVAE
                     KYGIPANWKLRAQAPFGGIAAPAADKDYMADSDRVKVFGN"
     gene            complement(4552..5223)
                     /locus_tag="U1099_000976"
     CDS             complement(4552..5223)
                     /locus_tag="U1099_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEM4867307.1"
                     /translation="MKKRNILVELLKGIGLVLLSTILWTLILSFATPESSSGIYEPFP
                     GFTVIAGILTSFILHIIMKYNQLRQAEQAAKASKSHIVVIEERNQALMKKANRLVEKH
                     QNLEKETILDINKSVSKIRTVEEKTVMKSTKIESSQEFGQFLRQMPELMANKHIERLL
                     NEIFETENNLAQWKISYNQQVEYFNTLLHQFPISVIAKIIRLKDLEFYKEHSLTDFTD
                     EMLGL"
     gene            complement(5235..9626)
                     /locus_tag="U1099_000977"
     CDS             complement(5235..9626)
                     /locus_tag="U1099_000977"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEM4867308.1"
                     /translation="MSDKFQLLLQQIGMPLDARQSGAFSTATIEKVVLHKVSKLWEFT
                     FRFETPLPLMDYQLFKARLATEFEKVGNKIQFSIVSDAEAFEAGLVEAYYPEAFTEDL
                     CQSAGFKALFQPLEVAYRDGVLWIKGPETIDTDHFRKNHLPNLVEQYKRFGFGNLAVD
                     IQVCQEMTQQQAEIFHAQNEEIYQQANEENLAALEQLAQMAPPPEAAQPFVPEYKKNR
                     PAKVNIEKAEITPMIEVDSEENRIVFEGLVFEVEQKTTKTGRVIINFKMTDYTSSFTL
                     QKWAKNEEEAQKFDMVKKGNWLRVRGNVETNNFTRDLTMNVQEVQEVKKEIRKDLMPE
                     GEKRVEFHAHTNMSTMDALPAVEDLVARAAAWGHKAVAITDHGNVQSFPHGYHAARKA
                     GIKPLFGMEANIVEDSVPIAYNEADVVLSDATYVVFDVETTGLSAVNNALIQIAASKM
                     HKGNIIAEFDEFIDPGHPLSQFTTDLTGITDEHVRGSKPLEQVLREFQDFCQDSVMVA
                     HNATFDVGFMNVNYERAGLPIISQPVIDTLEFARNLYPDFKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFLKDALEKHNLTNLNQLNTELIAEDSYKKARVKHATLYVIN
                     QVGLKNMFKLVSLSNTKYFEGVPRIPRTVLNAHREGLILGTACQEGEVFDDLLSKGID
                     EAVKTAAYYDFIEVMPPALYAPMIAKEQFKDKAEIEETIKQLIEVGRRAGLPVLATGN
                     VHYIDPEEEIYREIIVRALGQGAPINWTIGNGENAQPAPLPKAHFRTTSEMLDEFAFL
                     GESLAREIVITNPNAMLDRFEDVQVVKTDLYTPYIEKAEETVAELTYQKAFEIYGNPL
                     PDIIDLRIEKELTSILGNGFAVIYLASQMLVHRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPMPPHYVCPNCQHSEFITDGSYGSGFDLPDKDCEKCGTKYKKDGQDIPFETFLG
                     FDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEENAFRAGTVGTVAAKTAYGFVRGYERD
                     YGKFYRDVEVERLAAGAAGVKRTTGQHPGGIIVFPDYMDVYDFTPVQYPADDVTASWQ
                     TTHFNFHDIDENVLKLDILGHDDPTMVRKLQDLSGIDPQTIPADDKGVMALFSGTEIL
                     GVIPEQIGIPTGMLGIPEFGTNFVRGMVEETKPTTFSELLQLSGLSHGTDVWLGNAQD
                     LIKAGIANLSTVIGCRDDIMVYLMHAGLPPKMAFNIMERVRKGLWLKISEEERNGYIQ
                     AMKDNKVPDWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPLYYYCAYFSIRAK
                     AFDLATMSGGLERVKAKMEEIALKKKNNEASNVEQDLYTTLELVNEMLERGFKFGKLD
                     LYKSHATDFLIEDNTLIPPFVAMDGLGENVAKQVVAARAEGEFLSKTELRKRGGLSGT
                     LVEKMDEMGILGKMPEDNQLSLFDDLF"
     gene            complement(9750..10739)
                     /gene="dhaQ"
                     /locus_tag="U1099_000978"
     CDS             complement(9750..10739)
                     /gene="dhaQ"
                     /locus_tag="U1099_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEM4867309.1"
                     /translation="MVFIRNRQATIIEDYLDGFCRTNPKVKKVKGLPIVLQKVQDDHL
                     VPIISGGGSGHEPAHIGFVGKGMLTAAVYGEIFTPPTAEEVLAAIRAVDKGKGVFLIV
                     KNFEADLVSFRKAIELARQEGIAVKYIVSHDDISVDTKNNFRMRHRGLAGTILLHKIL
                     GAAALTGYSLDELEQLGLSLATEIATIGFATKSAYLPNAALPLFDLEEGQISYGIGIH
                     GEEGYRTVRFESSEQLANEIVNKLKLKFRWKEGEDYILLVNNLGSMSEVEQGIFLNDI
                     CQFLELEGLQLSYIKTGKFMTSLDMGGISVTLCRVKDRKWLDFLEAPTEAAAW"
     gene            complement(10750..11295)
                     /gene="dhaS"
                     /locus_tag="U1099_000979"
     CDS             complement(10750..11295)
                     /gene="dhaS"
                     /locus_tag="U1099_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEM4867310.1"
                     /translation="MPTSLITKKRIAKAFKKQLSQKSFDKISVVDIMQEAGIRRQTFY
                     NHFLDKYELLDWIFETELQEQVTDNLTYISGIKLLDELLYYIESNQSFYRQLFEIKGQ
                     NDFVSYLEGYFMVLMEKIINEYQLQEGKMISQQDKHFLILYHANAWIGLIKEGLTQSP
                     CQIHSYWKQMVALVRESFVIL"
     gene            11443..12432
                     /gene="dhaK"
                     /locus_tag="U1099_000980"
     CDS             11443..12432
                     /gene="dhaK"
                     /locus_tag="U1099_000980"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEM4867311.1"
                     /translation="MKKIINQPEHVVDEMLKGLVFMHQDLVDRIDGFDVIIRKAEKTG
                     KVGIISGGGSGHEPSHAGFVGKGMLSAAVCGAVFTSPTPDQILEAIKAADEGAGVFMV
                     IKNYSGDIMNFEMAQELAEMEGIEVDSVVVDDDIAVENSLYTQGRRGVAGTILVHKIL
                     GHAAETGKSLAEIKELADRLVPQIKTIGLALSGATVPEVGKPGFVLEEDEFEYGVGIH
                     GEPGYKKEKLQPSAKLAEELVEKLAEGFDIQDGEHYGVLINGLGATPLMEQYVFANDV
                     AQLLAKKGVVVDYKKIGNYMTSIDMAGLSLTLIKLEDPSWIDALQAPVEVVAW"
     gene            12443..13018
                     /gene="dhaL"
                     /locus_tag="U1099_000981"
     CDS             12443..13018
                     /gene="dhaL"
                     /locus_tag="U1099_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEM4867312.1"
                     /translation="MDAKQALLWMETFNKKIQEDKDYLSELDSPIGDGDHGGNMARGM
                     AAVMQELSEKDYETADQVFKVVAMQLLSKVGGASGPLYGSAFMGITKASQAGADLADT
                     LQAGLEMIQKRGKAELGEKTMVDVWIPVIAALRESQLTTEIIHKAVQGTKDIAATKGR
                     ASYVGERSIGHIDPGSFSSGLLFEAMLEAGL"
     gene            13015..13389
                     /locus_tag="U1099_000982"
     CDS             13015..13389
                     /locus_tag="U1099_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEM4867313.1"
                     /translation="MTKVGILLVSHSKNLAQGIIDLVSEVAKDIPITYCGGLEDGSIG
                     TSFEIVQERVEANPADTLLAFFDLGSARMNMELAADFTDKQILIQTVPVVEGCYTAAA
                     LLQAGADLETILEQLKELTINK"
     gene            13399..14109
                     /locus_tag="U1099_000983"
     CDS             13399..14109
                     /locus_tag="U1099_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM4867314.1"
                     /translation="MNLIGELLGTFILVLLGNGVVASCILSKTKAENSGWLTIVLGWG
                     IAVTIAAYVSGVFGPAHLNPAVTIAMASIGSLPWAEVPGFLLAQFAGAMLASIVLYLH
                     FYPHWQETKDSGTILATFSTGPALRHTASNLLGEALGTAVLVMGILAIGPNNVSAGLG
                     PIIVGLIVFAIGFSLGSTTGYAVNPARDLGPRIMHAILPIPNKGDSDWSYSWIPVAGP
                     ILGGVAGAILYNLLLNML"
BASE COUNT         4047 a         3339 c         2881 g         4000 t
ORIGIN      
        1 atatatccat ttgtaagcgt tgtcaactgt ttttgttgac ttttttcaaa aaaaatctat
       61 aatataacca aagatgttat aataatatca aagaaaggat ttccgacgat gagagacaat
      121 tcaacaaaat caccactata ttatcagcta ttcgatagtt tggtggaata tattcaaaaa
      181 gatttacaac cacatgatca attacctaca gaaaaagaaa ttggaactga ttattccgtc
      241 agcagaacaa ccgtacgatt ggccatgcaa gagttagaga aacatggata catttatcga
      301 attcaaggaa aggggtcgtt tgtatctagt cttaaacaag aaaatatcaa ctcatttttt
      361 tcacttgaat taaaaaatca ctatgatggg ttagaaaccg cagactttga atcccactta
      421 gtcagtttca caaaagaaaa cggttctcta gcattaaaac atctctttgg tctgtccaaa
      481 caaacaaatc tcgtccgcat acaattagaa cacctgcttc attcagaact tgttgctact
      541 gaaacaattg tagcaaaagc tagctatatg cctgaactaa cgcaggaaag tttggaattt
      601 gaaggattgg actcgttgtt ccaaaaaact ggtgctatca ctaaagctgt tgatgaacac
      661 tattctcttg ctagatttat gccaactagt tcagaaattg atgcattaaa aattacaaaa
      721 tctattttta accaagataa cgaattgatg ataacttcca ctagaattat caatatccaa
      781 aaactaccgt accgaaattt tatttggaca aactgaaatt aatagataga aaaaccgcag
      841 attgcttgaa tctacggctt tttatttaat attctctttt ttaaaccgac aactaattga
      901 aacaattctc attcacccca attttttata taaatcaatc atctctcgtg ccaaatctaa
      961 aaaagtaatt gcattcatta aatggtcttg actatgaatc attagtaaac caatggaaag
     1021 ggtgtcaccc gctgcttctc ttgtcaacat atccgtctgg acatgatgtg cttgaattaa
     1081 cgaatcgtga gcttgtctca ataaatcatc tgcttcatca aagcaacctt ccttagctac
     1141 ttgaatagct ctcatcccat gacccttagc atctccacta tacatgatga gttgcataat
     1201 aggcaataag tctttctcgt ctatattcat cactatctcc tatgataaat ctagtagaga
     1261 tttggcatat tcaattacct tagccccgtt catcgtacca taatcaagca tattgataac
     1321 tgcgatagga atattagcta aatgtggatc tttcttaaac tcctcctcca tataactaac
     1381 ttgtggtccc aacaaaataa tatctgctgg attcttttca acttctaata ccagctcact
     1441 caccgaaaca gcatagattg tagcttcaaa cccttgagcc tcagcagctt gttgcatctt
     1501 agtcaccaat aagctagtac tcataccagc agagcacgca agtacaattc gtttcatttt
     1561 tgattctcct ataaataatt atctattgat atggcgatag gatcgtacgt tgttataaca
     1621 actataacat ttttaaaaac tttttgcaag cgctctctta gacaattttt caatcagtgc
     1681 agttctaatc cctccgactg cggctaaaaa tagccaccag caatactgct ccaaagactg
     1741 acggtaccag ggccatacca gcaaactgtg ggccccaatc accaaagagc aactgtccaa
     1801 tccatgaacc tgcaagacca aggataatat ttcctagaca gccacgacgg tcatctgatg
     1861 aggtcaaggc tccggcaatc aagccaataa tagctccagc gataatgctt gttaacattc
     1921 tgtattcctc cttatttaca aaataagact ggttgcccag tcttataaga tgactattag
     1981 atagcccact caccgtcgcg gaagataggg acgcgagttc catcttcacg aatgccatca
     2041 atgttcattt tgtttgaacc aatcatgaag tcaacgtgga catcagaacg gttgagacct
     2101 gcttctttca actcttcttg gatcatttct gtaccacctt caattgagaa ggcataggct
     2161 tgcccgattg ccaagtggtt tgaggcattt tcatcaaaga gagtgttgaa gaaagttaca
     2221 cctgattgag agattgggct cttatctggt acaagggcaa cttcacctag accgcgtgca
     2281 ccagcattgt caaagaccag tttcttcatg acttcgtcac ctttttcagc agtcacatcc
     2341 acgatttcac catctttgaa ggttactttg atgccttcga tgatgttacc attgtaactg
     2401 agtggttttg ttgacgttac atagccgtct gccacacggt agtcaggagc tgtgaagacc
     2461 tcttctgttg gcatgttggc aataaagtgt tcaccttggg cattaaccga accagccgct
     2521 tcccacaagt gattcttcgg catacctaga acgaggtcag ttcctggtgc tgtgtagtgc
     2581 aatttgacaa actgctcgtc gttaagcacc ttagccttag ccaccaagcg tgcttcgtgc
     2641 tcttcccaag ccttgattgg atcttcctcg tagatacggc aagtcttgaa gatttggtcc
     2701 caaaggaggt ccactgcctc ttcgtcagac gctgcatttg ggaagacttt tttagcccat
     2761 tccaaaccag aggctgcacc gagtgtccag ctgattttgt tagcttggga tgcttgccgc
     2821 attgggttca aagcctggct gagtgcacga gtgttgcgag acaatttctc tggatcaacg
     2881 ccatcgtaag cacctggatc ttctgacaaa acaaccaaac ggctagcctt acgctccaag
     2941 aggtagttca tttcagtgat gcgttgtgga tgaacttgtt ccaagagttc cacatcaaca
     3001 ttgaccaaac gttcacgcgc aatcacatcg tccaaccagt tgaccaagac ttccttagca
     3061 ccgtgagcgt aagcttcttt gacaatcaag cgtgccaact cagcctgctc cacaccaatt
     3121 gtcaattgaa ccgtgtgacc aggttggaca ttgatacctg ttgaaaccaa gagtttagca
     3181 tatttagcga gattttcttt aaaatttggt aataccatct ttttctcctt tgtactttga
     3241 aataccctat tattgtatca tattttaagc tagacagata aaaaaacttc ctgctgatac
     3301 aggaagttga aaaggttagc tctgggagat cgttgaagct tggagatgaa acgaatggag
     3361 ttcgtgtcag caagccgcag gtataactca acaccctctt atttctaggc attgacttga
     3421 aatagtcccc cagattgctt acacagttaa tggactgtgc aaggtgggag ataaaacttg
     3481 cggagcaagt cgcttttgca gaatcaggca aggcgagttc aaacaatcca gtggattgtt
     3541 tgaagttgga aataaggaaa cgaagtttcc tcaactgccg acgtaataaa gcaaactgaa
     3601 ataaattata tatactcaat gaaaattgga atcagactag gcgacgtaga tgtagataga
     3661 acttaagttc atcacatcaa gtcaacaacg tctgacttca attttcgcag agtattagtt
     3721 accaaaaacc tttacacggt cactatccgc catatagtct ttatccgctg ctggagctgc
     3781 aatgccaccg aatggtgctt gggcacggag tttccagtta gctgggatac cgtatttttc
     3841 agcaacttgt gcatcaacca atgggttgta gtgttgtacg ttcataccga tatttttctc
     3901 agcaaaggcc aaccatactg cgtacaagtt gataccatga gcttgttcag accagattgg
     3961 gaagttttca gcgtaaagtg ggaagctttc ttgtaagcct ttcaccacgt cttggtcttc
     4021 gaagaacaag atagtaccgg caccagccgc aaaaccagct aagcgacctt ttgtagcttc
     4081 aaaagcttct gcaggcgtta ctttttccaa ttcgctgtag gcaatttcgt tccagaaagc
     4141 ttctgactcc gcaccaaaaa gtaccactgc acggctagat tgtgagttga aagctgatgg
     4201 agattctttc atggctttct caacaagtgc tactacttct tcatttgata ctggcaagtc
     4261 tttaccaagt gcgtagattg tacggcgaat ttcttgaagt tttgcaaagt ttgacatata
     4321 ttttcctctt ttcttattgt tactacatag tagtttttat cactataggg agattgtatc
     4381 attattacta agtagtgtca aattcctagt ctaacttttt tatactccgt gaaaattaag
     4441 ctagttacag cagatagtct gattttcata aagtagtgac agagaattta caaaaagatg
     4501 gcaaaaaagc ctatcaaata cttaagatac attgatagac ccttctttat cttatagccc
     4561 caacatctcg tctgtaaaat cagtcagcga atgttcctta taaaattcca agtccttcag
     4621 gcgaatgatt ttggcaatga cgctaatcgg aaactgatgc aacagagtat tgaaatactc
     4681 aacctgctga ttgtatgaaa tcttccattg tgctaaatta ttttctgttt caaaaatttc
     4741 attcagtaac ctttcaatat gtttattggc catcaattcc ggcatttgac gcaaaaattg
     4801 accaaattct tgtgaacttt caatcttggt ggatttcata acagtttttt cttcaaccgt
     4861 tcgaattttg gaaacggatt tattgatgtc caaaatggtt tctttttcaa gattctggtg
     4921 tttttcaacc aagcggtttg ctttcttcat caaggcctga ttgcgctctt caataacaac
     4981 gatatgactt ttactagctt tcgcggcttg ttcagcctga cgaagctggt tatacttcat
     5041 aatgatatgg agaatgaaac tcgttagtat accagcaatc acagtaaaac ctggaaaagg
     5101 ttcgtaaata cctgatgatg attccggtgt ggcaaaagat aagatcaatg tccacaagat
     5161 agtagaaagc agtactagac caattccttt taataactca actaaaatat ttcttttttt
     5221 catcatttcc ttttttaaaa caaatcatca aagagactca actggttatc ctctggcatt
     5281 ttaccgagga tgcccatttc atccattttt tcaacaagcg tacccgacaa tccaccacgt
     5341 ttacggagtt ctgtcttgga gagaaattct ccttcggcac gcgccgccac aacctgcttg
     5401 gcaacgttct ccccaaggcc atccatggca acaaatggtg ggatgagggt attatcttca
     5461 atcaagaaat cggttgcatg ggacttgtaa aggtccaatt tcccgaactt gaagccacgt
     5521 tccagcattt catttaccaa ttcaagtgtg gtatagagat cctgctcaac gttcgaagct
     5581 tcattgtttt tcttcttgag ggcgatttct tccatcttag ccttgactcg ttccaagcca
     5641 ccggacatgg ttgccaaatc aaatgccttg gcccgaatag agaaataagc gcaatagtag
     5701 taaagtgggt gatgaacctt gaaataggca acccgcaagg ccatcataac gtaggcagcc
     5761 gcgtgggctt tagggaacat atacttgatt tttccacagg attcgatgta ccagtctggc
     5821 accttgttat ccttcatggc ttgaatgtag ccatttcgct cttcctcaga aatcttgagc
     5881 cacaagccct tccgcacccg ctccatgata ttaaaggcca tcttaggtgg caaacctgcg
     5941 tgcatgaggt aaaccatgat gtcatcccga caaccgataa cagtagatag attagcaatt
     6001 cctgccttaa tcaagtcctg ggcattgccc aaccaaacgt ctgtaccgtg cgatagacca
     6061 gataactgca agagttccga aaaagtggtc ggtttggttt cctccaccat cccccgtaca
     6121 aagttggttc caaattcagg aatgcccagc atacctgtcg gtatcccaat ctgctctggg
     6181 ataacaccca gaatttccgt accagaaaaa agagccataa cccccttatc atcagcagga
     6241 atagtttgcg gatcgatgcc tgacaagtcc tgcaacttcc gaaccatggt tggatcatcg
     6301 tgccccagaa tatcgagttt caagacgttt tcatcaatat cgtggaagtt aaagtgggtg
     6361 gtctgccagc tggctgtcac atcatcggca ggatactgaa caggggtaaa gtcatagaca
     6421 tccatgtagt cagggaatac gataattcct cctgggtgct gaccagtcgt ccgtttgaca
     6481 ccagcagcac cagcagccag gcgttctact tccacatctc tatagaattt gccataatcc
     6541 cgctcatagc ctcggacaaa accataggcc gtcttggccg ccaccgtacc aaccgttcct
     6601 gcacggaagg cattttcttc accgaaaata tcccgaacat ccaaatgggc agacggttgg
     6661 tcatcccctg agaagttcaa gtcaatatcg ggaaccttgt ctccatcgaa accaaggaag
     6721 gtctcgaaag gaatgtcctg tccatctttt ttatacttgg tcccacactt ttcacaatcc
     6781 ttgtctggca ggtcaaaacc tgaaccatag gaaccatctg tgataaattc actgtgttga
     6841 caatttggac agacatagtg aggaggcata gggtttactt cggtaatccc aatcatggtc
     6901 gcaacgaaac tggagccgac agacccccgt gaacccacca ggtagccccg ctcgttggaa
     6961 cggtgcacca acatctgcga tgccaggtaa atcacggcga atccattacc cagaatggac
     7021 gtcaattcct tctcaatccg caggtcgata atatctggaa gaggattgcc ataaatttca
     7081 aaggctttct gataggtcaa ttccgcaacc gtttcctcgg ccttctcaat ataaggcgta
     7141 taaaggtcgg tcttaaccac ctgaacatct tcaaaccggt ccagcatggc attgggattg
     7201 gtaatgacaa tctcacgagc cagactttct cccaagaatg caaactcgtc caacatctcg
     7261 ctggtcgttc taaagtgggc ttttggcagt ggtgctggct gagcgttctc accatttccg
     7321 atggtccagt taatcggtgc cccttgacct agagcacgaa caatgatttc ccgatagatt
     7381 tcctcttcgg ggtcaatgta gtgaacattt cctgtcgcca gaacaggcag acctgcccta
     7441 cgtcctacct caatcaactg cttaatagtc tcttcaatct ctgccttgtc cttaaactgc
     7501 tccttggcaa tcatgggagc atagagggcc ggtggcatga cttcgataaa atcataatag
     7561 gctgccgttt taaccgcttc atctatccct ttggagagca agtcatcgaa gacctcaccc
     7621 tcttgacagg ctgttcccaa tattaaacct tcacgatggg cattgagaac ggtccgtgga
     7681 atacgtggaa ctccttcaaa atacttggta ttggaaagag agaccagttt gaacatattt
     7741 ttcaaaccaa cctgattaat cacatagagc gtggcatgct tgacacgagc cttcttatag
     7801 gaatcctccg caatcagctc cgtatttagc tggttgagat tggtcaaatt gtgcttctcc
     7861 aaggcatctt ttaggaagat gaagagcagg cgccctgtcg cctccgcatc gtagttagcc
     7921 atgtggtggt gttcaagagc aacaccaaac cgcttggtga gaggacctaa accatgacgc
     7981 ttaaagtcgg gataaaggtt acgagcaaat tccaaggtat caatgacagg ctggctaata
     8041 ataggtaagc ctgctcgttc atagttgaca ttcataaagc caacgtcaaa ggtcgcgttg
     8101 tgggcaacca tgacggaatc ttgacagaaa tcttgaaact cacgcagaac ttgttctaga
     8161 ggtttagaac cacgcacatg ctcatctgtg attcctgtca gatctgttgt aaattgactg
     8221 agcggatgac ccggatcgat aaactcatca aattctgcaa tgatattgcc cttgtgcatc
     8281 ttagacgccg caatctgaat cagggcattg ttaactgcag agagacctgt tgtttccacg
     8341 tcaaagacca cataggtggc atcagacagg actacatctg cttcgttgta agcaataggc
     8401 acagagtcct cgacaatatt agcctccatc ccgaagagcg gcttaatccc agctttacgg
     8461 gcagcatgat agccatgggg gaaggattga acattgccgt ggtcggtaat cgccaccgcc
     8521 ttgtgtcccc aggctgctgc acgcgccacc aagtcctcaa cggcaggcag ggcatccata
     8581 gtagacatgt tggtatgggc atgaaactca acccgcttct caccctcagg cataaggtcc
     8641 ttacgaattt ccttcttgac ctcctggact tcctgtacgt tcatagtcaa atcacgagtg
     8701 aaattattgg tctccacatt gcctcgcacc cgcaaccagt tgcccttttt gaccatatca
     8761 aacttctggg cctcttcctc attcttagcc cacttctgca aggtaaagga cgaagtatag
     8821 tcggtcatct taaagttgat aatgactcgc cccgtcttgg tcgtcttctg ctcgacctca
     8881 aagaccagtc cctcaaagac aatccgattc tcctcgctat ccacctcaat cataggcgta
     8941 atctcagcct tttcgatatt gaccttggca ggacgatttt tcttatattc tgggacaaat
     9001 ggctgagctg cctctggtgg tggcgccatt tgagccagtt gttcaagggc cgccaagttt
     9061 tcttcattag cctgctggta aatttcttca ttttgcgcat ggaaaatctc cgcttgttgc
     9121 tgcgtcattt cctgacagac ttggatgtcc accgcaagat taccaaaacc aaatcgcttg
     9181 tattgctcca caagatttgg cagatgattc ttccgaaaat gatctgtgtc aatggtttca
     9241 ggacctttta tccacaaaac accatctcta taagccacct ctaatggctg aaaaagagcc
     9301 ttgaagcctg cactctggca caaatcttcc gtaaaagctt ctggataata tgcttctacc
     9361 aaaccagctt caaaagcctc cgcatcgctg acaatagaaa attgaatctt attgcccacc
     9421 ttttcaaact ccgtcgccaa acgagccttg aagagttgat agtccatgag cggcaagggg
     9481 gtttcaaaac ggaaggtaaa ttcccaaagt ttactgacct tgtgcaagac tactttctca
     9541 atcgttgcag tcgaaaaagc ccctgattgt cgtgcatcaa gaggcattcc gatttgttgt
     9601 agcaagagtt gaaacttatc tgacatactt atttcctttg tagttcgtta gaaacatttt
     9661 accataaatg aggtattttt tcctgagtca ggaagaaaaa actctcaggt catttcctga
     9721 gagatctgat ttctatagaa attagtagcc taccaagccg cagcttctgt aggcgcctct
     9781 aaaaaatcca gccattttcg atccttaaca cgacagaggg tgacagaaat tcctcccata
     9841 tcaagactgg tcataaattt acctgttttt atataggaaa gctgtaaacc ttctagctct
     9901 agaaattggc aaatgtcatt caaaaagatt ccttgctcaa cctcagacat agatccaaga
     9961 ttattgacca gaagaatata gtcttccccc tccttccagc ggaattttaa ttttaatttg
    10021 ttcacaattt cgttggctaa ctgctcagag gattcaaaac gaacggtccg atagccttct
    10081 tctccatgga taccgatgcc gtaagatatt tgtccctcct ccaaatcaaa caaaggcagg
    10141 gcagcattgg gcaaataggc cgacttggtt gcaaaaccga tggtcgcaat ctctgttgcc
    10201 agagacagtc ccagctgttc caattcatct aggctatagc cagtaagggc agcagctcct
    10261 aatatcttat gaagtagaat ggtccctgca agccccctat gccgcatacg gaaattattc
    10321 ttagtatcga ccgaaatatc gtcatgggat acgatgtact tgactgcaat tccctcctgc
    10381 cgagccaatt cgatggcttt tctaaaagag acaaggtctg cctcaaagtt ttttacaatt
    10441 agaaaaactc ccttaccctt atctactgca cgaatggctg ccaatacttc ttcagcggtt
    10501 gggggcgtaa atatttcacc atatactgct gcagtcagca taccctttcc gacaaatcca
    10561 atatgtgccg gttcatgccc cgatccacca cctgaaataa ttggaactaa atggtcatcc
    10621 tgcactttct gaagcacaat cggaagtccc ttcactttct tcactttagg attggttcga
    10681 cagaaaccat ccaaataatc ttcaataatt gttgcctgtc tatttctaat aaacaccata
    10741 tattaactcc tacaaaatca caaacgactc gcgaacaaga gccaccattt gcttccaata
    10801 actgtgaatc tgacaaggtg actgtgtcag tccttccttt atcagtccaa tccaagcatt
    10861 agcatgatag agaatcaaaa aatgcttatc ctgttgggaa atcattttac cctcctggag
    10921 ctggtactcg ttgataattt tctccatcaa aaccataaag tagccttcta aatagctgac
    10981 aaaatcattt tgccccttaa tctcaaagag ttgcctatag aaagattggt tactctcgat
    11041 gtagtacaaa agctcatcca gaagcttgat tccactaata taggtcagat tgtcagttac
    11101 ctgctcctgc aattctgtct caaatatcca gtccagcaac tcatacttgt ctaaaaaatg
    11161 gttgtaaaag gtttgacgac gaatccctgc ttcctgcatg atgtcgacaa ctgagatttt
    11221 atcaaaggat ttttgggata gctgtttttt aaaagccttt gcaatccttt tctttgtaat
    11281 caacgaggtt ggcatatgtc acttcctttc gcttcctctt tattataccc tatcatgctt
    11341 ttctttggaa caaaagggac acattggaca atctgtccct tccttttttc ttttggacaa
    11401 tgtagaatga aagcgcaaac aaaaaaacaa aggagttttc tcatgaaaaa aattatcaat
    11461 caacctgaac atgtcgttga tgaaatgtta aaaggacttg tctttatgca ccaagatctt
    11521 gtcgatcgta tcgatggatt tgatgtgatt atccgcaagg ctgaaaaaac tggtaaagtc
    11581 ggcatcatct caggaggtgg ctctggtcac gaaccttctc atgcaggctt tgtcggcaag
    11641 ggcatgttgt ccgccgctgt atgtggcgca gtatttacct caccaacacc agaccaaatc
    11701 ttggaagcca tcaaggctgc tgacgaggga gctggcgtct ttatggtcat caaaaactac
    11761 tctggcgaca tcatgaactt tgagatggcg caagaattgg cggaaatgga gggcattgaa
    11821 gtggacagtg tcgttgtgga cgatgacatc gctgtggaaa acagcctcta tacccaagga
    11881 cgtcgtggtg tagcaggtac catccttgtc cacaagattc tcggtcacgc tgcagaaacg
    11941 ggcaaatcgc tggctgagat taaggaactg gctgaccgtc tcgtcccaca aatcaagact
    12001 atcggcttag ccctatcagg tgctacggta ccagaagtgg gcaaaccagg cttcgtcctt
    12061 gaagaggatg aatttgaata cggtgtcggt atccatggtg aacctggcta taagaaagaa
    12121 aaactccaac catctgctaa attggctgaa gaattggttg agaaattggc agaaggcttt
    12181 gatattcaag atggcgagca ctatggtgtc cttatcaacg gtctcggcgc aacccctctc
    12241 atggaacaat atgtctttgc caacgacgtt gctcaattgc tggctaaaaa aggtgtggtc
    12301 gttgactaca agaaaatcgg taactacatg acatcgattg atatggctgg actgtcattg
    12361 accttaatca aactagaaga cccaagctgg attgacgctt tgcaggcccc tgttgaagtg
    12421 gtagcttggt aggagaaaga ctatggatgc aaaacaagca ctgctctgga tggagacatt
    12481 caacaaaaaa attcaagaag acaaagatta tttgagtgag ctagattctc caattggaga
    12541 cggagatcac gggggcaaca tggcgcgagg aatggctgcc gtcatgcagg agctatctga
    12601 gaaagactat gagactgctg accaagtctt taaggtggtt gccatgcaac ttctcagcaa
    12661 ggttggcggt gcttccggtc ctctctacgg ctctgccttt atgggtatta ctaaagccag
    12721 tcaagctggc gctgatttgg cagatacgct tcaagcaggt ttggagatga tccaaaaacg
    12781 cggcaaggct gaattgggag agaaaaccat ggtagacgtg tggattcccg tcattgctgc
    12841 cctaagagaa agccagctga caacggaaat tatccataaa gccgttcaag gtacaaagga
    12901 tattgctgcg accaagggac gcgcgtccta tgttggggaa aggtctattg gacacattga
    12961 tccaggctct ttctcatctg gacttctctt tgaagccatg ttggaggcag gactatgaca
    13021 aaggttggta tcttactcgt atcccattca aaaaatttgg cccagggaat catcgaccta
    13081 gtatctgaag tcgcaaaaga tattcccatc acctactgtg gcggactaga agatggtagc
    13141 atcggtacca gctttgaaat cgtacaagag cgagttgaag caaaccctgc tgataccctc
    13201 cttgcctttt tcgacctagg tagcgcccga atgaacatgg aacttgctgc cgattttaca
    13261 gacaaacaaa ttcttatcca aaccgtacca gtcgtcgaag gttgctatac tgccgcagct
    13321 ctcttacagg ctggagctga cttggagact attcttgaac aattaaaaga actgacaatc
    13381 aataaatagg aggtcattat gaatctgatt ggagaattac ttggaacttt catactagta
    13441 ttacttggaa acggtgtggt tgcatcctgc atcctctcta aaaccaaggc tgaaaatagc
    13501 ggctggctga ccatcgtact aggatggggc atcgccgtaa caatcgctgc ctacgtatca
    13561 ggagtttttg gacctgccca cctgaatcca gctgtcacta tcgcaatggc atctatcggc
    13621 tccctacctt gggctgaagt cccaggcttc ctactcgctc aatttgcagg agcaatgttg
    13681 gcatccatcg tcctctatct ccatttctac ccacattggc aggaaacaaa agatagcgga
    13741 acaatccttg ctaccttctc tactggacca gctcttcgtc acacagcttc aaacttgctc
    13801 ggcgaagctc ttggtacagc tgtcttagtc atgggaatcc ttgcaattgg tcctaacaat
    13861 gtcagcgctg gcctcggccc aatcatcgtg ggacttatcg tatttgcaat cggcttctca
    13921 ctaggatcaa cgacaggcta cgcagtcaat ccagctcgtg acttaggccc tcgtatcatg
    13981 cacgctatcc ttccaattcc taacaaggga gattctgact ggtcttatag ctggatccca
    14041 gttgctggcc caattcttgg aggtgtcgca ggagcaatcc tctataacct cctactaaat
    14101 atgttataaa aaaataggct tttggaaaaa gaccagcgca acttatcaga gttcgtatca
    14161 acatctcagc gcagtggttg attggcagat ttattcgtgt ttcacactcc aaatctgacc
    14221 tatacgactg aagcgaacag agttcgcttc atctccaacc tcaaact
//
LOCUS       DASFSW010000045        13614 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.045, whole genome shotgun sequence.
ACCESSION   DASFSW010000045 DASFSW010000000
VERSION     DASFSW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.045"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(40..435)
                     /locus_tag="U1099_000984"
     CDS             complement(40..435)
                     /locus_tag="U1099_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM4867315.1"
                     /translation="MYNKVILIGRLTAQPELTQTPNGKNLTRVTVAVNRRFKTENGER
                     EADFLNVIFWGKLAETLVSYGNKGSLISIDGELRTRKYEKDGSNHYVTEILGQSFQLL
                     ESRAQRAMRENNAGDDLADLVLEEEELPF"
     gene            490..1269
                     /locus_tag="U1099_000985"
                     /pseudo
     CDS             490..1269
                     /locus_tag="U1099_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
     gene            complement(1303..1926)
                     /locus_tag="U1099_000986"
     CDS             complement(1303..1926)
                     /locus_tag="U1099_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEM4867316.1"
                     /translation="MIFTYNKEHVGDVLMMIVAEDKGQPVQFERKGQVARVFLEETGK
                     TVAWNIFEASSLVELAGNGQVFMTDEQVATLNAELAKEGFTESLVNDNSPKFVVGQIV
                     ELVPHPDSYHLNICQVNVGDKTVQIVAGAPNAAQGLKTIVALPGAMMPSGSLIFPGKL
                     RGEDSFGMMCSPRELALPNAPQVRGIIELDDSAVVGEALDPAKHWKG"
     gene            complement(1945..2901)
                     /locus_tag="U1099_000987"
     CDS             complement(1945..2901)
                     /locus_tag="U1099_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEM4867317.1"
                     /translation="MKKSLGKILLAPVVLGLSLSLAPLVQAEIQTDVINEKWGKPTLV
                     YGGGLSDAQATEVNNLFGISDVNNVSRQVVVGTDMDQYLGTSGADTASLYSSVLVQKQ
                     DAGKGVVVDIKTPQNITLITETQYANAAITAGATDVLIDVAAPIQVTGESALTGVYKA
                     LAANGDTVDTARTEVAQQELTTVNEVATAHAGDEQFDSSALDKAVAEIKTALADYKKS
                     NGQVASEADINTIINDVLTQNGLENVITADEISKLVTFAKAYQETSAIDSAEVAAQLN
                     QFKQQAEQQISEAYKNLQDSGILEKIGAFFENLWKGLTGLFA"
     gene            complement(2946..3266)
                     /locus_tag="U1099_000988"
     CDS             complement(2946..3266)
                     /locus_tag="U1099_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEM4867318.1"
                     /translation="MIFPTNLEEVAGLIEDGKPTVFLFVTTWCGDCHYIKPHLSAIEE
                     AFPDFRFVQLDRDDFMPLAQEWAILGIPSLVVLENGQEIGRFVDKNRKTKEEIMNFLS
                     GLGR"
     gene            complement(3263..3547)
                     /locus_tag="U1099_000989"
     CDS             complement(3263..3547)
                     /locus_tag="U1099_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEM4867319.1"
                     /translation="MKKKRTALLAGLLGAGVLTASAQFYRKVQEDRKKAQALQEVRDF
                     FANVGEIATVYVDETASTQDSLVGGVVMEAGQVFLFENAQGSIQYREEER"
     gene            3760..4821
                     /gene="pepA"
                     /locus_tag="U1099_000990"
     CDS             3760..4821
                     /gene="pepA"
                     /locus_tag="U1099_000990"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEM4867320.1"
                     /translation="MTLFEKIKEVTELQSLPGFEGQVRNHIRQKITPHVDRIETDGLG
                     GIFGIKDTTVENAPRILVAAHMDEVGFMVSQIKPDGTFRVVELGGWNPLVVSSQAFTL
                     QLQDGRTIPAISGSIPPHLTRGGNAPGVPAIADIIFDAGFTNYDEAWAFGVRPGDVLI
                     PKNETILTANGKNVISKAWDNRFGVLMVTELLESLSGQALPNQLIAGANVQEEVGLRG
                     AHASTTKFNPDIFLAVDCSPAGDIYGDQGKIGDGTLLRFYDPGHIMLKNMKDFLLTTA
                     EEAGVKFQYYCGKGGTDAGAAHLKNHGVPSTTIGVCARYIHSHQTLYSMDDFLEAQAF
                     LQAIVKKLDRSTVDLIKNY"
     gene            complement(4867..6123)
                     /locus_tag="U1099_000991"
     CDS             complement(4867..6123)
                     /locus_tag="U1099_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEM4867321.1"
                     /translation="MNYQETRRWLSSRPASDLENGVARVNWILERLGNPQLQVPTIHF
                     VGTNGKGSTLNALQSILQSSGYTVGRFTSPSIIDFREQIVFEQEMISEKDFARIVTDL
                     QPLIEDLDQTAGLDAISEFEIVVVAMFVYFAHYQRPDILLVEAGMGGLQDATNVLAPL
                     AVVCPSIGLDHQIFLGETHAAIARHKVAVLRERVPLIYAIDLPEVEAVFEDHASQLQS
                     PTYAVGREILLEDSRAGFAVSSPLGRVEELKVLMQGRHQEVNAALAVTTAQLLSSHFP
                     KIINETISQGLSQAIWPGRLELMQPWLMIDGAHNNESIAVLTQLLEEKYADRDIEILF
                     AAINTKPVDQMLSQLSQFGPVSVTTFDDFRAVQLDDYPSGYERVQTYQEWLEQADLDN
                     PKKLYLITGSLYFITYVRKYILEEFI"
     gene            complement(6134..6730)
                     /locus_tag="U1099_000992"
     CDS             complement(6134..6730)
                     /locus_tag="U1099_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEM4867322.1"
                     /translation="MEKKIPKLTVQLLAAIAVTLALVMIVENYFSVRLSDTLQVQFTF
                     IPNAILGAIAGPVWAAVFAAISDPVFVLFSGQSMLFSFILIEAVSAFIYGWFFYRKPL
                     DTKNKADWLYVAGVVVLIRVVISFIMTPIALHFHFGTPWIALYSSRFIKAIFEIPLRI
                     VVTMLVLPSLQKIPELAKLVGIKQNSIKQQVISLLLLL"
     gene            complement(7045..8232)
                     /locus_tag="U1099_000993"
     CDS             complement(7045..8232)
                     /locus_tag="U1099_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEM4867323.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVLAAGIVERIGLNDSISTV
                     KYDGKKESEVLDIPNHTVAVKILLEDLLKHNIIAAYEEITGIGHRVVAGGEIFKESAV
                     IGPKEVEQIEELSALAPLHNPAHVAAMRAFEEVLPEVLNVAVFDTAFHTTMPKHAYLY
                     PIPQKYYTDHKVRKYGAHGTSHYYVAHEAAKVLGRPIEELKLITAHIGNGVSITANYH
                     GESVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIANVEELKDAADVVNMLNKQSGLFG
                     VSGFSSDMRDIEAGIQAHNPDAVLAYNIFIDRIKKFIGQYLAVLNGADAIVFTAGMGE
                     NAPLMRNDVVEGLSWFGIELDPEKNVFGNYGDISTPESKVRVLVIPTDEELVIAREVE
                     RLK"
     gene            complement(8282..9235)
                     /locus_tag="U1099_000994"
     CDS             complement(8282..9235)
                     /locus_tag="U1099_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM4867324.1"
                     /translation="MNFEKIEQAYDLLLENVQTIQNQLGTNIYDAMIEQNAAYVANQH
                     EMDLVVNNNKTLKQLDLTKEEWRRAYQFLLIKANQTEPMQYNHQFTPDSIGFILSFLV
                     DQLMPTQKVTVLEIGSGTGNLAQTILNASQKELDYLGIEVDDLLIDLSASIADVMQAD
                     ISFAQGDAVRPQILKESQVILGDLPIGYYPDDQIASRYQVASPNEHTYAHHLLMEQSL
                     KYLEKDGFAILLAPNDLLTSPQSDLLKGWLQEQANIVAMIALPPNLFGKAAMAKSIFV
                     LQKKAARSLTPFVYPLQSLQEPEAIQKFMLNFKNWKQENAI"
     gene            complement(9292..9702)
                     /locus_tag="U1099_000995"
     CDS             complement(9292..9702)
                     /locus_tag="U1099_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEM4867325.1"
                     /translation="MLLKKKVKAGILLYALLMLAVFSLLLQFYLHRQEAESRLVQVAR
                     QETTAYIMAQMVLDKVEQDLQVEKIVTGAVVNEKEANLTTGSKQKENRGRVSFQEGQA
                     RYQLKGDQLLVTVELTSDGLYTYQFPLGLSSEGG"
     gene            complement(9680..10114)
                     /locus_tag="U1099_000996"
     CDS             complement(9680..10114)
                     /locus_tag="U1099_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEM4867326.1"
                     /translation="MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLVQEAHYQGQ
                     TVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGEQALAFGKSWSDDFRKTNDRGQG
                     YQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS"
     gene            complement(10101..10394)
                     /locus_tag="U1099_000997"
     CDS             complement(10101..10394)
                     /locus_tag="U1099_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HEM4867327.1"
                     /translation="MVAIRKQKNKAYILLESLVALATLVTICSLILSAVDVGRRRQAW
                     ELEQQEVLNLAQMAVQTGQDKLALNGVTVQVQQTADKIIVFYEGKEVLSVVKK"
     gene            complement(10366..10773)
                     /locus_tag="U1099_000998"
     CDS             complement(10366..10773)
                     /locus_tag="U1099_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEM4867328.1"
                     /translation="MLRRKNKAFTLFESLLTLLVVSFLAISLSGTVQTVFRSVQEEIF
                     LWEFEAIYKDSQKLAASSRQAVSLSIGGQEVSNGSQIVKVPRNVEILEEKAIRFEEDG
                     GNSSLTKIRFRLSQKIVTYQLYIGSGRYKKTEE"
     gene            complement(10754..11035)
                     /locus_tag="U1099_000999"
     CDS             complement(10754..11035)
                     /locus_tag="U1099_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEM4867329.1"
                     /translation="MKKLVEMKVKGFTLVEMLVVLGIISLLLLLFVPNLSQQKDAIQK
                     KGDAAVVKVVESQMELYELEHDKEATVADLQAAGYITEKQAKQYAAAKK"
     gene            complement(11037..12002)
                     /locus_tag="U1099_001000"
     CDS             complement(11037..12002)
                     /locus_tag="U1099_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEM4867330.1"
                     /translation="MARQRKVIELFNNLFASGFHLGEIVDFLKRSQLLADLYTQVLSD
                     GLLAGKPFSSLLADLRFSDAVVTQVALAEVHGNTSLSLSHIQSYLENVSKVRKKLIEV
                     ATYPIILLGFLFLIMLGLKNYLLPQLEEGNAATMLINHLPTIFLSLCGLSLVAVLAGM
                     VWFRKTNKIKAFSRLAALPFFGKLIQIYLTAYYAREWGSLIGQGLDLPQIVGLMQEQQ
                     SQLFREIGQDLEQSLSNGQSFHEHIKTYAFFKRELSLIVEYGQVKSKLGGELTVYAAE
                     CWEDFFSRVNRAMQLIQPLVFLFVALMVVLIYAAMLLPIYQNMEL"
     gene            complement(11986..12936)
                     /gene="tadA"
                     /locus_tag="U1099_001001"
     CDS             complement(11986..12936)
                     /gene="tadA"
                     /locus_tag="U1099_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEM4867331.1"
                     /translation="MIQEKARKLIEKAVADRVSDIYLVPRGESYQVYHRIMDEREFVQ
                     DLAEEEVTAIISHFKFLAGLNVGEKRRSQQGSCDYDYGSGEISLRLSTVGDYRGKESL
                     VIRLLYDNDKELKFWFEAAERLAEEIKGRGLYLFSGPVGSGKTTLMYHLARLKFPDKQ
                     ILTIEDPVEIKQEDMLQLQLNEAIGATYDNLIKLSLRHRPDLLIIGEIRDAETARAVI
                     RASLTGATVFSTVHARSISGVYARMLELGVSPEELNNALQGIAYQRLIRGGGVVDFAK
                     GNYQNHSADQWNAQIDRLFAAGHISLRQAETEKIALGSPA"
     gene            13022..>13614
                     /locus_tag="U1099_001002"
     CDS             13022..>13614
                     /locus_tag="U1099_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEM4867332.1"
                     /translation="MKTLKKGDHIRVVSPSSSIERIGGFEANLAAKEKLEDLGFKLSF
                     SEHYFENDIFDSAPIASRVADLEVAFADESVDAILTTIGGFNCNELLPYLDFDLIARH
                     PKIFCGYSDTTALLNAIYAKTGMPTYMGPAYSSFKMDLGQQYQTEAWLKAVTQDSFDL
                     TPSPEWSSDAWYLPDAPRTFYPTEWKVYNPGQASGIA"
BASE COUNT         3799 a         3344 c         2716 g         3755 t
ORIGIN      
        1 cctatttttc aatacgctcc atcggtcttg ggactgatat taaaacggta attcctcctc
       61 ttctaagacc aaatcagcta gatcatcgcc agcattattt tcacgcatag cacgttgggc
      121 ccgactttcg agcagctgga aggattgtcc taagatttct gtcacatagt ggttgctgcc
      181 gtctttttcg tattttcgcg ttcgcaattc accatcaatg gaaattagac tacctttatt
      241 accataggaa acaagcgttt ccgccagttt gccccagaaa atgacattaa gaaaatctgc
      301 ttcacgttcg ccattttccg tcttaaaacg gcggttgact gcgacagtaa cacgggtcag
      361 atttttgccg ttaggggttt gagtgagttc aggttgggcc gtcaagcgac cgattaaaat
      421 aactttatta tacattttat atcctcctac ttatttattc gtagaaggat tagaaaagga
      481 gcgaaaatta tgaaagaatt tttgatggaa aagatacaca cctcctaacc atatactaat
      541 gccgaactag cttggttgct ggaaaacatc agtcatcctg atcctgctat acgcgacgaa
      601 ctggtttatg ctagtttttg ccacatcttt ttggagggct taatcacaag ggagcaagca
      661 cagtcgctac tacaattctc tcaagaaacc aatcaccttt ccctagaaag ttccacacta
      721 aaacgaagtt ttacttgctt gctctactgt cttttgctat ctgttgataa tgagcgagat
      781 tctgtgtact ttgcattttt aagcactaac gaccgtgagc tactcttcca gcaggccttg
      841 gattatttgg cgattgaaaa tgactggtct ggctacgatg aaaaactcgg ctggatccac
      901 accgctgccc acggagcaga ttttcttctg gccacaagct gtcatgatca atttcccgct
      961 gaaaaaagca aagaagtctg gcaaactatt gtcacttgtc taactagaca aagtaaagtt
     1021 ttctcagctg gcgaggaaat ccgtctggca caaattcctg tatatttact actgaatgaa
     1081 aaagtaacca gcaaagagtt gacagaatgg attagcaagc tggactttcc aaatcaagaa
     1141 ccagtagact acttccggtg gctcaatctc caacattttc tgtctagtct ttacttccaa
     1201 cttaggtcac atcaagcatc gaccgaagaa atcgaacagg ctattgaagg aaaaattgaa
     1261 ccagcataaa agaattggcc aaagcgacca attcttcttt ttctatccct tccagtgttt
     1321 agctggatca agtgcttctc cgactactgc ggagtcgtcc aattcgataa tgccacggac
     1381 ttgtggtgcg tttggcagag ccaattcacg cggactacac atcataccaa agctatcttc
     1441 accacgcaat ttccctggga aaatcaggct accgctcggc atcattgccc ccggaagagc
     1501 cacaatagtt ttcaatcctt gggcagcatt tggtgctcca gctacgattt gaacggtttt
     1561 gtcaccaaca ttgacctgac agatattgag gtggtagctg tctggatgcg gaaccagttc
     1621 cacaatctga ccgacaacga atttcggact attgtcgttg actaagcttt cagtaaaacc
     1681 ttccttagcc aattcagcat tcaaggtcgc aacttgctcg tctgtcatga aaacctgacc
     1741 atttccagca agctcaacca aacttgatgc ttcaaagata ttccaggcta ctgtttttcc
     1801 tgtttcctct aggaaaacac gcgccacttg acccttgcgt tcaaactgaa caggctgacc
     1861 cttgtcttca gccacaatca tcataagaac atcgccgacg tgttccttat tatatgtaaa
     1921 aatcatttct ttttcatttc tcctttatgc aaatagaccg gtcaagcctt tccaaagatt
     1981 ttcaaagaag gcaccgattt tttctaaaat ccctgaatct tgtagattct tataggcttc
     2041 tgaaatttgt tgctccgcct gttgcttgaa ctggttgagt tgggcagcaa cctcagccga
     2101 atcaatagca gaggtttcct gataagcttt ggcaaatgtg actagcttag aaatttcatc
     2161 tgctgtgatg acattttcta aaccgttttg ggtcaaaaca tcattgatga tggtgttgat
     2221 gtcagcctca gaagccacct gaccattgga ctttttgtag tcggcaagag cagtcttgat
     2281 ttccgccact gccttatcca aagcagagct gtcaaattgt tcatcaccag catgggctgt
     2341 cgctacttca ttaacagttg ttaattcttg ttgagctact tcagttcgag cggtatcaac
     2401 tgtgtcacca ttggctgcaa gagccttgta aacacctgtc agagcggact ctcccgtcac
     2461 ctgaatgggt gccgcaacgt caatcaagac atctgtcgca ccggcagtaa tggccgcatt
     2521 ggcatactgc gtttcagtaa tcaaagtgat gttctgcggt gtcttaatgt ccaccacaac
     2581 ccctttgcca gcgtcctgct tttgcaccaa gacagatgaa tagagggagg ctgtgtccgc
     2641 tccagatgta cctaaatatt ggtccatatc cgtgccaaca accacctgac ggctaacatt
     2701 attaacatcc gaaataccaa atagattgtt gacctctgtc gcctgcgcat ctgacaaacc
     2761 tccaccgtag actagtgttg gcttgcccca tttttcattg ataacatctg tttgaatctc
     2821 agcctgcacc aagggagcaa ggctcaaact caaccccagc accacaggtg ctagaaggat
     2881 ttttcctagt gatttcttca tgttctttcc tttcttttgg gaaatggcag agcttctctg
     2941 ctaatttatc tacccagtcc agataagaag ttcatgattt cttccttggt cttgcgattc
     3001 ttatcgacaa aacgaccgat ttcctgacca ttttccaaaa caactagact tggaatgcct
     3061 aaaatagccc attcctgcgc caaaggcata aagtcatctc ggtctagttg cacaaaacgg
     3121 aaatctggaa aagcctcttc gatagctgac aaatgcggtt tgatatagtg gcagtctcca
     3181 caccaagtcg tcacaaatag aaaaacagtc ggcttaccat cttcaatcaa gccagctact
     3241 tcttcaagat ttgttggaaa aatcatcttt cttcctccct gtattgaata gagccttggg
     3301 cattttcaaa tagaaacacc tgtcctgcct ccatcacaac tcctccaacg agagagtctt
     3361 gcgttgaggc tgtttcatcc acatagaccg tcgcaatttc tcctacatta gcgaaaaagt
     3421 cacgcacctc ctgcaaagcc tgtgcttttt tacggtcttc ctgaacctta cggtaaaatt
     3481 gagcactagc ggtgagcaca cctgccccca ataagcctgc tagcaaggct gttcttttct
     3541 ttttcatgtt gactatttta tcatatttcc tctgaatttt cccaaaaatg ccattggact
     3601 tgtgacagct gagacaaact ctccacaaac atatgaaaaa gattcagttt tcagaaagca
     3661 tgatttcatc atgtgtttat tgtatatttt tccatatttt caagacaagt ctatctcttc
     3721 gtgataaaat agtagtaatc atgtcatgga ggaagaagaa tgactctttt tgaaaaaatt
     3781 aaagaagtta cagaattaca aagcttacca ggctttgaag gacaggttcg caaccatatc
     3841 cgccagaaaa tcacaccgca tgtagaccgc atcgaaacag atggactagg tggaattttt
     3901 ggcatcaagg atacaactgt tgaaaatgct cctcgcatct tagtggctgc acatatggat
     3961 gaagtcggct ttatggttag ccagatcaaa ccagacggta cttttcgtgt tgtcgagcta
     4021 ggtggttgga accctttggt cgtatcgagt caagccttta cgcttcaact acaggacggt
     4081 cggaccattc ctgccatttc aggttccatc cctccacatc taacacgcgg gggcaacgct
     4141 ccaggcgtac ctgccatagc tgatattatt ttcgatgctg gttttaccaa ttatgacgaa
     4201 gcttgggcgt tcggcgttcg tccgggggat gtcctcattc ccaaaaatga aaccattcta
     4261 acagccaacg gaaaaaatgt catttctaag gcttgggaca accgcttcgg tgtccttatg
     4321 gtgaccgaac tactagaaag cctatccggt caagcgttgc caaatcagtt gattgctggg
     4381 gcaaatgtgc aagaagaggt tggtcttcgt ggtgctcatg cctctacaac caagttcaat
     4441 ccagacattt tcctcgctgt tgattgctcg cccgcaggtg atatttatgg cgaccaagga
     4501 aaaattggtg acggaactct gcttcgtttc tatgatccag gtcatatcat gttaaaaaac
     4561 atgaaagact tcctcctcac aactgccgaa gaagcaggtg tcaaattcca atactactgt
     4621 ggtaaaggtg gaacagatgc cggtgcagcc cacttgaaaa accatggtgt tccatcaaca
     4681 actattggtg tctgcgctcg ctacatccat tcacatcaaa ctctctatag catggatgac
     4741 ttcttagagg ctcaagcctt cttgcaagct atcgttaaaa agctagatcg ctcaacggtt
     4801 gatttgatta aaaattatta aaaacaaaag actttgctag gtagaacgcc cagcaaagcc
     4861 ttttttctat ataaactctt ctaatatata cttcctcaca taagtaataa agtaaagcga
     4921 gcctgtaatc aggtaaagtt ttttgggatt gtccaagtcc gcctgctcca accactcttg
     4981 ataggtctga actcgttcat aacctgacgg ataatcatcc agctgtaccg cacggaaatc
     5041 atcaaaggtt gtcacactaa caggtccaaa ttgactaagc tgcgataaca tctgatccac
     5101 aggcttggta ttgatggctg caaagaggat ttcgatgtct ctgtccgcat atttttcctc
     5161 taggagctgg gtcaggacgg cgatactttc attattgtgg gctccgtcaa tcatgagcca
     5221 tggttgcatc aattctaagc ggcccggcca gatggcttgg gacaagccct ggctgatggt
     5281 ttcattgata atctttggaa aatgtgagct aagaagctga gctgtggtca ctgccaaggc
     5341 tgcattgacc tcttggtgac gcccctgcat caagactttc aactcttcca cacgaccgag
     5401 aggacttgaa actgcaaagc ctgctctgct atcttccaaa agaatctccc gccccaccgc
     5461 ataggtcgga ctctgaagct ggctggcgtg atcctcgaaa actgcctcca cttctggcaa
     5521 atcgatcgca tagatgagag gaaccctctc acgcaagacg gcaaccttgt gacgggctat
     5581 ggctgcgtgg gtctctccca aaaaaatctg atgatccaag ccgatggagg gacaaactac
     5641 tgccaagggg gcaaggacgt tggtcgcatc ctgcaaaccg cccatgcctg cctccaccaa
     5701 gagaatatcg ggacgctggt agtgggcaaa gtagacaaac atagccacta ctacaatctc
     5761 aaactccgag atggcatcca gtccagccgt ctggtccaag tcctcaatca agggttgcaa
     5821 gtctgtcaca atcctcgcaa aatctttctc cgaaatcatc tcttgctcaa agacaatctg
     5881 ctctcgaaaa tcaatgatag acggtgaggt aaagcgaccg acggtgtaac ccgaagactg
     5941 caagatagac tgtaaggcgt tgagggtcga gcccttgcca tttgtaccaa caaagtgaat
     6001 agtcggcact tgaagctggg gattgcccaa gcgttccaaa atccagttga cacgtgcaac
     6061 gccattttct aaatctgatg caggacgact agatagccac cggcgagttt cttgataatt
     6121 catggttccc tctctacaaa agcagcaaca gggagatgac ctgttgcttg atactgtttt
     6181 gtttaatgcc cactaacttg gccaattcag gtattttttg taaacttggc aagacaagca
     6241 tggtaacgac aatgcgtaat ggaatttcaa aaatagcttt tatgaaacga ctgctataca
     6301 gggcaatcca aggtgttcca aaatggaaat ggagggcaat cggtgtcatg ataaaggaaa
     6361 tcacaacccg aatcaagaca acaacccctg caacatagag ccaatcagcc ttgttcttgg
     6421 tgtctagcgg ttttcgatag aagaaccagc catagataaa agccgatacc gcctcaatca
     6481 agataaaact aaaaagcatg ctctgtccac taaacaagac aaagactggg tctgaaatcg
     6541 ccgcaaacac agctgcccaa acaggaccag caatcgcacc taaaatagca ttagggataa
     6601 aggtaaactg gacctgcaag gtatcagaaa gacgaacaga aaaatagttc tccacaatca
     6661 tgaccaaggc aagggtcaca gcaattgcag ccaacaactg caccgttagt tttggaattt
     6721 tcttttccat aaaaaatgct cctttttcaa aaaatggagc tgtcgtgtgt attcgacagc
     6781 ggatgcaatg gtttaccgcg ctactctcag ccaggaccaa tgttcctact ataggagaca
     6841 tcagacacaa gcaaagatca gcttcgttat gtggcaacat cccatccaca tacacttgac
     6901 gcaaaccatc tactctgtgc aaatttcttt gctagtctat tgtatcacac actatatatt
     6961 ttgaccactt gctaaaaact atacctttct aaaggtataa actataagag aaactggcag
     7021 tctccgacta ccagttagtt tttcttattt caagcgttcc acttcacgcg caataaccaa
     7081 ttcttcatcc gttggaataa ccaagacacg aacttttgat tctggcgttg aaatgtcacc
     7141 atagttgccg aatacatttt tttccgggtc caactcaata ccaaaccaag acaagccttc
     7201 tactacgtca ttgcgcataa gcggtgcatt ttcacccata ccagccgtga agacaatagc
     7261 atctgcccca tttaaaactg caagatactg accgataaat ttcttaatac ggtcaatgaa
     7321 aatattgtag gccaacactg catctggatt gtgagcttgg atacctgctt caatatcacg
     7381 catatcactt gagaagccag atacaccgaa taagcctgac tgtttattca acatattaac
     7441 aacgtctgca gcatctttca gttcttcaac attcgcaatc aaataaggaa tgatagcagg
     7501 atcaatatcc cctgaacgag ttcccatcat tggaccagct agtggtgtga agcccattga
     7561 agtatccaca gattcaccat gatagttagc agtgatggat acaccatttc cgatatgtgc
     7621 cgtaatcaat tttaactctt cgattggacg ccccaatact tttgctgcct catgcgctac
     7681 ataatagtga ctagttccgt gagccccata cttacgaacc ttgtggtcgg tatagtactt
     7741 ttgtggaatc ggataaagat aggcatgctt tggcatcgta gtgtgaaagg ctgtatcaaa
     7801 aacagctaca ttcaaaactt ctggtagtac ttcctcaaat gcacgcattg ctgctacgtg
     7861 agcagggtta tgaagcggtg ccaaagcact tagttcttca atttgctcaa cctcttttgg
     7921 tccaataaca gctgattctt tgaaaatctc tccacctgca acaacacggt gtccgatacc
     7981 agtaatttcc tcgtaagcag cgatgatgtt gtgtttaagc aagtcttcta ggagaatctt
     8041 aaccgcaacc gtatgattag gaatatctaa aacttcactt tctttcttcc catcatactt
     8101 aactgttgag atggaatcat tcaaaccgat gcgctcaaca atccctgccg ctaatactgt
     8161 ttcttctggc atttgataaa gttgccattt tagacttgaa ctacctgcat taattgcaat
     8221 tgtttttgac atatcttacc tcttttaaag cgttttccta actagtataa cagattttca
     8281 cttaaattgc attctcttgc ttccaatttt tgaaattgag catgaacttc tgaatagctt
     8341 ctggttcttg aagactttgt aagggataaa caaacggcgt caacgatctt gcagctttct
     8401 tttgcaagac aaaaatagac ttggccatag cagccttccc aaagagattt ggtggcaggg
     8461 caatcatggc aacaatattg gcttgctcct gtaaccaacc tttcagcaaa tcgctttgcg
     8521 gactagtcaa taaatcattt ggagccaaca aaatcgcaaa gccatctttt tccagatatt
     8581 tcagggattg ttccatgagt aaatgatggg cgtaggtatg ttcatttggg ctggcgacct
     8641 gatagcggct agcaatctgg tcatctggat agtagccaat aggcaaatct cccaaaatta
     8701 cttgactttc cttcaaaatc tgcggacgta ccgcatctcc ctgagcaaaa gaaatatctg
     8761 cctgcatgac atccgcaata cttgccgaca aatcaatcaa gaggtcgtcc acttcgatcc
     8821 ccaagtaatc caattctttc tggctggcgt tgagaatggt ctgagctaga ttgcctgtcc
     8881 ccgaaccaat ttccagaacc gtcacctttt gagtcggcat caattggtct actagaaaag
     8941 ataggataaa tccgatagag tctggtgtga actggtgatt gtactgcatg ggttcagtct
     9001 gattggcctt gatgagcaag aattgatagg cacgacgcca ttcttccttg gttaaatcta
     9061 gttgtttcaa ggtcttgtta ttattgacaa caaggtccat ctcatgctga ttagctacgt
     9121 aagcagcatt ttgctcaatc atggcatcat agatattggt acctagctgg ttttggatag
     9181 tctgtacgtt ttctaatagc aggtcgtaag cctgttcgat cttttcaaaa ttcatactcc
     9241 tatcttatca aaatttcaca gactttgcac caaaaaggag agcgagtcaa ctcaccctcc
     9301 ttctgaggat agccccaaag gaaattgata ggtatacaga ccatcacttg ttaactcaac
     9361 tgtaaccaat agttggtctc ctttaagttg ataacgagcc tgtccctctt gaaaggagac
     9421 ccttcctcta ttctccttct gcttagaacc tgtggttaaa tttgcttcct tttcattcac
     9481 taccgctccc gtaactattt tttctacttg caggtcctgc tcgaccttat ccaagaccat
     9541 ctgagccatg atgtaggcag tcgtttcttg ccttgccacc tgtaccaagc gactctcagc
     9601 ctcttggcgg tggagataaa actgaagcaa gagactaaaa accgccagca tcaaaagggc
     9661 atagagtagg atgccagcct taactttttt ctttaaaagc atagataaag gtccgctcct
     9721 ctccgttttt aaagctaaag tcgatgcgca ccagctggtt ttcttgggaa atggctgcgc
     9781 tatccacctg atagagcatg ggttggtaac cctggcctct atcatttgtt ttccgaaaat
     9841 catccgacca tgacttgcca aaggccaaag cctgttcacc tttgttgaca tagaccttgc
     9901 cattttcaac cttgaccaat tcagcctggt cccactccga ccgcaattgg ctagagaaga
     9961 ccaaccattc cttttgaacg gtctgacctt gataatgcgc ttcttggacc agcagttttg
    10021 acaagccttc aaagaccaga agactacctg aaaggatgac taaggctacc aagcattcca
    10081 agagagtaaa agcaggaatt ttacttttta acaaccgata aaacctcctt tccctcgtag
    10141 aaaacaatga tcttatccgc tgtttgttga acctgaactg taacaccgtt taaggctagc
    10201 ttatcttgcc cggtctgaac agccatctgt gctagattga gaacctcctg ttgttccaat
    10261 tcccaagcct gtcgcctacg acctacatcc acagctgata aaatcaagct gcaaatggtc
    10321 actagagttg caagggcaac cagactttct agcaaaatat aagccttatt cttctgtttt
    10381 cttatagcga ccactcccta tatataattg atacgtgact attttttgac taaggcgaaa
    10441 acgaatcttg gtcagagaag aatttccacc atcttcttca aatcggatag ctttctcctc
    10501 caagatttcc acgtttcttg gcactttcac tatctgactg ccatttgaca cttcttgtcc
    10561 accgatagaa agactaactg cttggcggga actggctgcc aatttctgac tgtctttata
    10621 gatggcctca aattcccaga gaaaaatttc ttcctgaaca gaccgaaaga ccgtttgtac
    10681 tgttcccgac aggctgattg ccagaaagct gacaaccaac aaggtcagca gactttcaaa
    10741 cagggtaaaa gccttatttt ttcgccgcag catactgttt tgcttgtttt tcagtgatgt
    10801 agccagctgc ctgcaagtct gccaccgttg cttccttgtc atgttccaat tcatagagct
    10861 ccatctggct ctccactact ttgacaacag ccgcatcccc cttcttctga atagcatcct
    10921 tctgctggct caaatttggc acaaaaagga gcaagagcag gctaatgatc cccaaaacga
    10981 ctaacatttc caccagagtg aaccctttta ccttcatttc aactaatttt ttcattttac
    11041 aactccatat tttgataaat cggcagcaac atggctgcgt agatgagaac gaccattaag
    11101 gccacaaaga gaaagaccag cggttgaatc aactgcatgg ctctattgac ccgagagaaa
    11161 aaatcctccc aacactcggc tgcataaact gtcaactcgc cacccaactt ggacttgacc
    11221 tgaccatact cgacaatcaa actcagctcc cgcttaaaaa aggcgtaggt cttaatgtgt
    11281 tcgtgaaagc tctgcccatt ggaaagcgac tgctccaggt cctgcccaat ctcccgaaag
    11341 agctgcgatt gctgctcctg catcaagccc acaatctgtg gcaggtccaa gccttgccca
    11401 atcaaacttc cccactccct ggcgtaatag gccgtcagat agatttggat gagttttccg
    11461 aaaaatggca gagctgccaa gcgggaaaat gccttgattt tgttggtttt gcgaaaccaa
    11521 accataccag ccaagaccgc caccaaacta agtccacaga gggataaaaa gatagtcggc
    11581 agatggttaa ttagcatggt cgcagcattg ccttcctcca actggggcag aagatagttt
    11641 ttcaagccta gcataatcaa gaacagaaaa cccagtaaga taatcggata ggtcgccacc
    11701 tcaatcagct ttttacgaac cttgctgaca ttttccagat aggactggat atggctcaaa
    11761 ctcaggctgg tattaccatg aacttctgcc agagccacct gtgtgaccac cgcatctgaa
    11821 aaccgcaaat ctgccagtaa actcgaaaag ggtttgcctg ccagcaaccc gtctgacaag
    11881 acctgggtat agagatctgc cagaagctga ctgcgtttga ggaaatcaac aatctccccc
    11941 agatgaaaac cactagcaaa aagattattg aaaagctcaa tgaccttacg ctggcgagcc
    12001 aagggcaatt ttttctgttt ctgcctgcct aagactgata tgtcctgctg caaaaaggcg
    12061 atcaatttgc gcattccact ggtctgcgga atggttttgg taatttccct ttgcaaaatc
    12121 taccacacct cctcccctga ttaaacgttg ataggcaata ccttgaaggg cattgtttaa
    12181 ctcctcagga ctgaccccca attccaacat acgagcatag acacccgaaa tagaccttgc
    12241 gtgaaccgtt gagaagaccg tcgctcctgt cagactggct cgaatgactg ctcgagcggt
    12301 ttccgcatcc cgaatttcac cgatgatgag caagtctggt cgatgacgga gggacagttt
    12361 gatcagattg tcgtaggtgg ctccgatggc ttcattgagc tggagttgca gcatgtcctc
    12421 ctgtttgatt tcgacaggat cctcgatggt caaaatctgc ttgtctggga atttcagcct
    12481 ggcaagatgg tacataagtg tggtcttacc agagccgact ggacccgaaa aaaggtagag
    12541 ccctcgtccc ttgatttctt ctgcaagccg ctcagccgcc tcaaaccaga acttgagttc
    12601 cttgtcattg tcatagagca agcggatcac cagactttcc ttgccacgat aatctccgac
    12661 agttgataag cgaagtgaaa tctctccgct accataatca tagtcacagg aaccctgctg
    12721 gctacggcgt ttttcaccaa catttaaacc tgctaaaaac ttgaaatggc tgatgatggc
    12781 tgttacttcc tcctcagcca agtcttgcac aaactcccgt tcatccatga tgcgatgata
    12841 aacttgatag ctttccccgc gaggaaccag ataaatgtca ctgaccctat ccgccactgc
    12901 cttttcaatc aactttcttg ctttttcttg aatcataaaa cctcctcacc ctatctattc
    12961 gcaaggaaac ttgattttcc atactttttt gttaaaataa taaaaaaact tggaggttcc
    13021 tatgaaaaca ttaaaaaaag gcgaccacat acgtgtagtc agtccctctt catctattga
    13081 aagaattggt ggttttgaag ccaatcttgc tgccaaggaa aaattggaag acctgggctt
    13141 caaactctct ttctcagaac attattttga aaacgatatt ttcgattccg ctccaattgc
    13201 cagtcgtgtt gcggacttgg aagtagcctt tgcggatgag tcagtcgatg ccattctgac
    13261 aaccattggc ggtttcaact gcaacgaact cttgccctat ctggattttg acctaattgc
    13321 gcgtcatcca aagattttct gtggttattc ggacacaact gccctgctca acgccatcta
    13381 cgccaagaca ggcatgccaa cctacatggg tccagcttat tcaagtttca aaatggattt
    13441 ggggcaacag tatcaaacag aagcctggct caaggctgtg acacaagact cctttgactt
    13501 gaccccgagt ccagaatggt ctagcgacgc ttggtaccta ccagacgccc ctcgcacctt
    13561 ttacccaaca gaatggaaag tctacaatcc aggccaggcc tctggtattg ccat
//
LOCUS       DASFSW010000046        13499 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.046, whole genome shotgun sequence.
ACCESSION   DASFSW010000046 DASFSW010000000
VERSION     DASFSW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13499
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.046"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..428
                     /locus_tag="U1099_001003"
     CDS             <1..428
                     /locus_tag="U1099_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM4867333.1"
                     /translation="SQAVEPSSWSKGWFAQDLINLDEEKTAFENVSCTSLQDRMTILN
                     LLGMLGLSYDKAQQKVANLSGGERVRLSLAKVLLADANLLILDEPTNFLDMAAIEALE
                     TFLKEYEGSVLLVSHDQQFVETSVHRQWEIVQACLVKKS"
     gene            520..2595
                     /locus_tag="U1099_001004"
     CDS             520..2595
                     /locus_tag="U1099_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEM4867334.1"
                     /translation="MKKRKNKPLEHDGNFISLRLNILFSIVIFLFLVLILRLADMQII
                     NHDFYSNKLSTASQKIISNGSIRGQIYDAQGKPLVENEIQQVVSFTRPNKMSAQEMKE
                     VANKLLQWVNVSDVTITQRDKADYYLADEEVYRQVVEALPKDKKYDSDGNYLSESTIY
                     SNAVDSIPAEALQYSEQDNKAIELFKQMNGATYFSTVNLVTDTLTSEQVAHIVANESQ
                     LPGISTTNNWQRTILPTSLSSIIGTVTSEQAGLPAEDAEYYLSKGYSSNDRVGTAYLE
                     KQYEEVLQGQREKKQINLDRNGNIESIETIQEGQQGNNIKLTIDLAFQDGVNAILRKY
                     FESELATGSALYSEGVYAVALNPSTGAVLAMSGYSHEKGSSTITEDALGTITSVFTPG
                     SIVKGATISSGWENGVISGNQVQLDEPIYFAGSAPITSWYGAYGSFNIDATEALEYSS
                     NIYMVKIALGLLGQTYSANMYLNDGDTLKNAMTKLRSTFAEYGLGASTGIDLPLESTG
                     FLPDKYTTANFITNAFGQFDNYTPMQMAQYAATIANNGTRISPHLVEGIYGNNAQGGL
                     GELIETVSGKEMNQVNISAEDMSLLRQGFYQVVNGSGRFNTGSAIGRGAAVTISAKTG
                     TAETYTTTASGDVVTAINTNVVAYAPSDNPQIAVAVVLPNLTNQSSTTTKTITQEIIN
                     LYQSLHPMN"
     gene            2604..3200
                     /gene="recR"
                     /locus_tag="U1099_001005"
     CDS             2604..3200
                     /gene="recR"
                     /locus_tag="U1099_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEM4867335.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMEDDVVNEFA
                     KNLLAAKRDLSYCSICGNLTDQDPCAICQDSTRDQSTILIVEDSRDVTALENIQEYHG
                     LYHVLHGLISPMNGIGPDDINLKTLLTRLMDNEVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            3305..4351
                     /locus_tag="U1099_001006"
     CDS             3305..4351
                     /locus_tag="U1099_001006"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEM4867336.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIS
                     QTGDFIKTQEFSQTPSADEKLTTNATIVEEQKIRPSDIYEEKAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKRVLESAGISQVPYVAVIEGENIDDKIAE
                     IEGKLTYPVFVKPANMGSSVGISKAEDLAGLHKALDLAFKYDSRVLVEQGVNAREIEV
                     GLLGNADVKTTLPGEVVKDVAFYDYDAKYIDNKITMEIPAKIDEAIMDTMRDNAAKAF
                     RAIGGCGLSRCDFFLTEDGDIFLNELNTMPGFTQWSMYPLLWDNMGLAYPDLIEELVR
                     LAKEMFEKRESHLI"
     gene            4439..4819
                     /locus_tag="U1099_001007"
     CDS             4439..4819
                     /locus_tag="U1099_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEM4867337.1"
                     /translation="MYQTIVKGEGLFQALSQGYGESIRTFGVTEKGETPVSLVNIGLA
                     ACITMCVQGYYASQEGNKTMPVQVESRLDNQRFEVLIGIGESVSAQKQKEILAYVEKK
                     CKVKALLREDLQFETSFYVLESVE"
     gene            4821..5897
                     /locus_tag="U1099_001008"
     CDS             4821..5897
                     /locus_tag="U1099_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867338.1"
                     /translation="MFLAIEEMRQNKLRYGLILGLLILISYLVFFLTGLAYGLMQENR
                     TAVDKWQADYVLLNSESNRLITASKIDTSLLDQVDASDKALIRQQAGVAYVDENATSD
                     EKEKVNIFAVESDSFIVPNIVEGRLYEKTGEVLVDKTLSEVEGFGLGDQIYLSGSDEK
                     VTIVGYTDNAYFGVAPVVYMDFTAFAELMQADKQQAATSNLASAIVVRGDAASLPNEL
                     EKVAIADFIENLPGYKAQNMTFGFMIGFLIVISAIVIGIFIFVLTTQKSPIFGLMKIQ
                     GLSNGYISGSVLAQTFLLAGVGTVLGLAGTYLSSLVLPSAVPFENNWTFYIAIGLALV
                     VFALLGASFSVRSIFKVDPLRNLS"
     gene            5907..6575
                     /locus_tag="U1099_001009"
     CDS             5907..6575
                     /locus_tag="U1099_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867339.1"
                     /translation="MKTLIFENISKTFQDGSQTITALKPINFSIEEGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPTTGRVLINQSDYSDLPEKKRAKLRYKDIGFVLQASNLIPFLTV
                     EKQLTLVDKVNKKADPSKRNELLSELGVDHLKNKFPKDLSGGERQRVAIARALYNDPA
                     LILADEPTASLDSNRAFEVVELLAKEAKERNKSIIMVTHDQRMIEKCDKVYEMKDGVL
                     TQIR"
     gene            6944..8305
                     /locus_tag="U1099_001010"
     CDS             6944..8305
                     /locus_tag="U1099_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEM4867340.1"
                     /translation="MKLTLHEVAQVLGAKNDISLYPDRAFNKVEFDSRLITEGDLFVP
                     LKGARDGHDFIPVAFENGCVVTLSEVSLDVPHILVEDCLAALQQLAAYYLEKTGVEVI
                     AVTGSNGKTTTKDMIHDVLATTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKLVLEMGQ
                     DHLGDIHLLSEIAKPSLSVITLFGEAHLEFFGSREEIAKGKLQIADGMEPGSKLLIPA
                     YPIADKLLPSHVDLIRFGEQADLQVTSLVESKDSLTFTVNFMDGDIFLPVTGKYNATN
                     AMVASYVGKTLGVSNEAIKSALASLNLTRNRTEWKKAANGADILSDVYNANPTAMRLI
                     LETFSSIPANEGGKKIAVLADMKELGSQSVELHNQMILSLSPDVLDTVIFYGQDIEGL
                     AQLASQMFPIGKVYFFRKNAEQDQFGDLVKQVKEVLGGKDQILFKGSNSMNLGKVVEA
                     VEK"
     gene            8396..9094
                     /locus_tag="U1099_001011"
     CDS             8396..9094
                     /locus_tag="U1099_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEM4867341.1"
                     /translation="MSDFFSNQISEAPNLNVLEHLFLMIIWFFMLHLCFRCYQKRWFH
                     IVFWWLQFIQIVSLYTWYIVADSPLSESLPFYHCRLAMLFILWAKPGPLKRYFAYLGL
                     FGSLAAFIHPVFDPFAFPHLTFFTFVIGHYALTVNCMLYLLSDLEGEMLKGKEVVKYT
                     LIMNMLILGVALLTGGNYGFLRQAPLVNTNNLPLNFFLVTCLLCFSILSIQAMLIAYL
                     KKESKQMNSHILKK"
     gene            complement(9125..11461)
                     /locus_tag="U1099_001012"
     CDS             complement(9125..11461)
                     /locus_tag="U1099_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM4867342.1"
                     /translation="MNKQIKGLSTKEVQERIQNQQTNHFKTKTSASNWEIFRRNVFTS
                     FNALNFAIFLALLAVQAWSNLFFFGVIVLNAVSGMLTEWRARRMIDKLNLMNKDFIRV
                     VRDSKTLSLAPEDIVLDDVLLLSAGEQVPSDAIVLEGIAEANEAMLTGESDLIVKNTG
                     AELLSGSYLVSGQIYAKVIHVGAENYANKLMLEAKTHKPIVSRILYNMDKIAKFTGKI
                     IIPFGLALFLEAFFIKLLPLKDSVITSSTALLGMLPKGIALLTITSLLTAVIKLGMKN
                     VLVQEMYSVETLARVDVLCLDKTGTITQGKMTVENLLPLTNHYSLDTIQQILATYIQT
                     SEDTNSTAQAIRKEYGDLEHHYKASHIIPFSSDRKWGAMTIENIGRIFLGAPEMLLTQ
                     NPPSVSEAQARGSRVLILALSQQSLPSSQNQLPENIEPLALLEIADPIREDAAETLAY
                     LRSQEVTLKIISGDNPVTVSHIAKEAGFADYDSYIDCSKVDDEELIARAETTAIFGRV
                     SPHQKKLLIQTLKAQGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLD
                     SEFRDIPEILFEGRRVVNNISHIAPIFLIKTIYSFLLGLICIASIALGKAEYLLVFPF
                     IQVQMTLIGQFVEGFPPFILTFERNIRPVEKHFLRKSLLLALPNALMVVLSVLIFHLM
                     QVFGYLNLHDMQTLSYYVLGSTGLLAVIRACLPLTKARLALIIYSVFGFFISSHFLHG
                     LIEIHPLNSHTLPIYSGLMLIIIPVFFWISYKQGAFKN"
     gene            11698..13242
                     /locus_tag="U1099_001013"
     CDS             11698..13242
                     /locus_tag="U1099_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEM4867343.1"
                     /translation="MSLQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGNFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLQELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYRGDERFASLDEGDKLFGSNPFYAQVLDD
                     IELLAEAGNEFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTTGD
                     VIDPLNKDFSGFVFKIQANMDPRHRDRIAFVRVVSGEFERGMAVNLPRTGKGAKLSNV
                     TQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
BASE COUNT         3936 a         2570 c         3078 g         3915 t
ORIGIN      
        1 gctcccaagc agttgagcct tcaagctggt caaaaggttg gtttgcacag gatttgataa
       61 accttgatga agaaaagact gcttttgaaa atgttagttg tacctctctt caagatagga
      121 tgacgattct caatttatta ggtatgttag gactttctta cgataaggct cagcagaagg
      181 tagcaaatct gtctggtggt gagcgtgttc gtctctcctt agctaaggta ttgttggcgg
      241 atgcgaatct tttgatatta gatgaaccga caaattttct agatatggca gctattgagg
      301 ctttggaaac attcttgaaa gaatatgaag gaagtgtcct cttggtttcc catgatcaac
      361 agtttgtgga aacatctgtt cacaggcaat gggagatagt gcaagcttgt ctcgtgaaga
      421 aatcataaga aaatattttc tgaaaaggag gctctgtcta aaaaatattt tttgcctgtg
      481 gtataataag aaggatattt ctagaatgag gtctttgaag tgaaaaaaag aaagaacaag
      541 cctttggaac acgatggaaa ttttatttcc cttcgtttaa atattttatt ttctatcgtg
      601 attttccttt tcctagtttt gatattgcgt ctggcagata tgcagatcat caatcatgat
      661 ttttatagta ataaactgtc tactgccagt cagaaaatca tcagcaatgg ctctattcgc
      721 gggcagattt acgatgcgca gggaaaacct ttggttgaaa acgagattca gcaggtcgtc
      781 tccttcacac gtcccaacaa gatgtctgct caggaaatga aagaagtagc taataaactc
      841 ttgcagtggg tcaatgtttc agacgtaaca attactcaac gtgataaggc tgattattat
      901 ttggcagatg aagaggttta tcgtcaggta gtcgaggcct tgccaaaaga taaaaaatac
      961 gattccgatg gtaactactt gagcgaatca acaatttatt ctaatgcagt tgatagtatc
     1021 cctgccgaag cattacaata ttctgagcaa gacaataaag cgattgaact cttcaagcag
     1081 atgaatggag ctacttactt ctctactgtt aatttggtaa cggatactct gacttctgaa
     1141 caggttgctc atatcgtagc caatgaaagt caacttccag gcatttcaac gaccaataac
     1201 tggcaacgga cgattttacc aacttccctc agctccatta tcggtacagt aacgagtgaa
     1261 caggctggtt tgcctgcaga agatgcagaa tactatctat ccaagggcta ctcttcaaat
     1321 gaccgtgtgg gaactgccta cctcgagaaa caatatgaag aagtcttgca aggtcaacgt
     1381 gagaaaaaac aaataaacct agatagaaat ggaaatatcg aaagtattga gacaatccaa
     1441 gaaggacagc agggaaataa tattaaactg actattgatt tagcctttca agatggggtc
     1501 aatgctattt tgagaaaata ctttgaaagt gagctagcta ctggtagtgc gctttactcg
     1561 gaaggtgttt atgcagttgc attaaatcct tctacaggtg cagtacttgc tatgtcagga
     1621 tatagtcacg aaaaaggttc aagtactatt acggaggatg ctcttggtac cattacaagt
     1681 gtctttacac caggttccat cgtcaaagga gcgacaatca gctctggttg ggaaaatgga
     1741 gttatcagtg gtaatcaggt tcaattagat gaaccaatct attttgcagg ttcagcacca
     1801 attacatctt ggtatggggc ctatggtagt tttaatattg atgcgacaga ggctctggaa
     1861 tattcttcta acatctatat ggttaagatt gctttgggac tactcggtca gacctactca
     1921 gctaacatgt atttgaatga tggtgataca ttgaaaaacg ccatgactaa gcttcgttcg
     1981 acttttgcag aatatggtct tggtgcttct acaggtattg accttccact cgaatctaca
     2041 ggatttttac cagataagta tacgacagcc aactttatta ccaacgcttt tggacagttt
     2101 gataactata caccgatgca gatggcacag tatgcagcga ccattgcgaa taatgggaca
     2161 cggatttctc ctcacctcgt tgagggaatt tatgggaata atgcccaagg tggtttggga
     2221 gaattaattg agaccgtatc tggtaaggag atgaatcagg ttaatatctc cgctgaggat
     2281 atgtcacttc ttcgtcaagg tttctaccaa gttgtcaatg gtagtgggcg ctttaataca
     2341 ggtagtgcta tcggtcgtgg tgcggctgtg accatcagtg ccaagactgg tacagccgaa
     2401 acctatacaa caacagcttc aggagatgtg gttactgcga tcaacaccaa cgttgttgct
     2461 tatgcgccta gtgataatcc tcaaattgcg gtagcagttg tcctaccaaa tttgaccaat
     2521 caaagttcaa caactactaa aacaattact caagaaatta tcaatttata tcaatcactt
     2581 cacccaatga attaaggagg cttatgcttt accctacacc tattgccaag ttaattgata
     2641 gctattctaa attgccaggt atcggtataa aaacggctac ccgtttagct ttttatacta
     2701 ttggcatgga ggatgatgtc gttaatgagt ttgcaaaaaa tttattggca gccaagcgag
     2761 acctatctta ttgctctatt tgtggtaatt tgacagatca ggacccctgt gctatttgcc
     2821 aggactcaac gcgagaccaa tctactattt tgattgtaga ggatagtcga gatgttacag
     2881 ccttggaaaa tattcaagaa taccacggtc tttatcatgt cttgcatggc ttgatttctc
     2941 ccatgaatgg tattggacca gatgatatta atttgaaaac cctcctcact cgcctgatgg
     3001 acaatgaggt tacagaagtt attgtggcga ccaatgcaac agcagatgga gaagctacat
     3061 ctatgtatat ctcacgtgtc ctcaaacctg cgggaatcaa agtaacccga ctagctcgtg
     3121 gcttagcggt aggcagtgat attgaatatg cagatgaagt gaccctgttg agggctattg
     3181 aaaaccgcac agagctatag ctgttctgta gttagatacg ttcctaattt taagatagga
     3241 tagatatgat ttgttaaatt atgctatact aagagtaata gcgttgatac aggaaggaaa
     3301 atatatgtca aaagaaacct tgattttact atatggtggg cgttctgcag agcgtgaggt
     3361 atctgttctg tctgccgaaa gcgtcatgcg tgctatcaat tatgataaat tttttgtcaa
     3421 aacctatttc attagccaga caggggactt tattaagacg caagagtttt ctcaaacacc
     3481 gtctgccgat gaaaaactca cgacaaacgc gacgattgtt gaagagcaaa aaatccgtcc
     3541 gagcgatatt tatgaagaaa aggcggttgt tttccctgtt cttcatggcc caatgggaga
     3601 agacggatct attcaaggct tcttggaagt gcttcgtatg ccttatgttg gcactaatat
     3661 cttgtcttca agcgttgcta tggacaaaat cactaccaag cgcgtacttg aatcagcagg
     3721 aatctcccaa gtaccttatg tggcagttat cgagggtgaa aatattgacg ataagatcgc
     3781 agagattgaa ggaaaattga cttatccggt ctttgtaaaa ccagctaata tggggtcaag
     3841 tgtcggtatc tcaaaagctg aggatttagc tggcttgcat aaggcacttg atctggcctt
     3901 caagtatgac agccgtgtcc tcgttgagca gggtgttaat gcgcgtgaga ttgaggtcgg
     3961 tcttttggga aatgctgacg tgaaaaccac ccttcctggc gaagtagtca aagacgttgc
     4021 tttttatgac tacgatgcca aatacatcga caacaaaatc accatggaaa tcccagctaa
     4081 gattgacgag gctatcatgg acaccatgcg tgataatgct gccaaggcct tccgtgccat
     4141 cggtggttgt ggactttctc gctgtgattt cttcctgaca gaagacggcg acatcttcct
     4201 gaatgagctc aataccatgc caggctttac ccagtggtct atgtatccac tcctttggga
     4261 taatatgggc ctagcttatc cagacttgat tgaagagctt gttcgtttgg ccaaagaaat
     4321 gtttgaaaaa cgcgaaagtc acctcattta attattatca agattggaat cattccaatc
     4381 ttttttcttg caaaggtttg caaaaaggaa taaaatggaa caagttctgg aggtgtttat
     4441 gtatcaaacg atagtaaaag gggaaggtct gtttcaggct ctttctcaag gttatggcga
     4501 atcaattcgt acctttgggg taacggagaa gggtgagact ccggttagtc tagttaatat
     4561 tggcttagct gcctgtatca ccatgtgtgt gcagggctac tatgccagcc aagaaggcaa
     4621 taaaaccatg cctgttcagg tggagagtcg cttggacaat cagcgttttg aggtgttgat
     4681 tggaattggt gagtcggttt cagcgcagaa gcagaaagag attcttgcct atgtggaaaa
     4741 gaaatgtaaa gtcaaagctc ttttgaggga agacttgcag tttgagacaa gtttttatgt
     4801 cttagaaagt gtggagtaag atgtttttag caattgaaga aatgcgacaa aataagctgc
     4861 gttatggctt gattttagga ttattaatcc tgatttccta cctggtcttt ttcctgacgg
     4921 gtttggctta tggtctcatg caggaaaata gaacagcagt tgataaatgg caggcagatt
     4981 atgtcttgct taattcagaa agcaatcgct tgataacagc atccaaaatt gatacgagtc
     5041 tgcttgatca ggtggatgct agtgacaagg ccttgattcg ccaacaagct ggagttgcct
     5101 atgtggatga aaatgccacc tcagacgaaa aagaaaaggt aaacatcttt gctgttgaat
     5161 cagatagttt tatcgttcca aatattgtag aaggtcgttt gtatgaaaaa acgggagaag
     5221 tccttgtaga caaaaccttg tcagaagtag aaggttttgg gcttggagat caaatctatc
     5281 tgtcaggttc tgatgaaaaa gtgactattg taggttatac agacaatgct tattttggag
     5341 tggcgcctgt tgtatatatg gactttacgg catttgcaga attgatgcag gctgataaac
     5401 aacaagctgc aacgagtaat cttgctagtg ccattgtcgt tcgtggcgat gccgcttctc
     5461 taccaaatga gctggaaaaa gttgccattg cagactttat tgaaaatcta ccaggatata
     5521 aggcacaaaa tatgaccttt ggctttatga taggattctt gattgtcatt tctgccattg
     5581 ttattggtat ttttatcttt gttttgacaa cacagaaatc tccgattttt ggcttgatga
     5641 aaattcaagg tctttcaaat ggctatattt caggttctgt attggctcaa accttcctac
     5701 ttgctggtgt gggaacagtc cttggattgg ctgggactta tttgtcatcc cttgtcttgc
     5761 caagcgctgt tccatttgag aataactgga ccttctatat tgcaatcggc ctagcactag
     5821 ttgtatttgc cctattgggt gctagtttct ctgtacgttc tatctttaag gtagatccat
     5881 tacgaaatct atcttaggag gttgttatga aaacacttat ttttgaaaat attagtaaga
     5941 ccttccaaga tggcagccaa accattacgg ctcttaaacc aataaatttt agtattgaag
     6001 aaggcgaatt tgttgccatc attggaccat ctggttctgg gaagtcaaca tttttgacct
     6061 tggcaggtgg tttacaaaca cctacaacgg gtcgtgtttt aatcaatcaa tcggactatt
     6121 ctgatttacc tgagaaaaaa cgtgccaaat tgcgctataa ggacattggt tttgtcttgc
     6181 aggcttccaa tttgattcca tttctaacag tcgaaaaaca attaaccttg gttgataagg
     6241 tcaataagaa agctgatccg agcaagcgaa acgaactcct atctgaattg ggtgtggacc
     6301 atctcaaaaa caagttccct aaggatttgt caggcggaga acggcagcgt gtagctattg
     6361 ctcgtgcact ttataacgat ccagctttga ttctagctga tgaaccaaca gccagtctag
     6421 acagtaatcg cgcttttgaa gtggttgagt tattagctaa ggaagccaaa gaacggaaca
     6481 aatccatcat catggtaaca catgaccagc gcatgattga aaaatgcgac aaggtttacg
     6541 agatgaaaga tggtgtcctc actcaaattc gataaaaaat ggtataatgg gagcgggaaa
     6601 gaactcgact tgtcaaaaaa gagttcgtca acaagttttt atttctagtt gttgagctga
     6661 aacagtctat tcccagactg tttcactccc acccccgcac agctcaaact gtctgggaga
     6721 cagtttgagg ttggaaataa agcgaacact gatcgcatca gtaggtagga cagatttgga
     6781 gtgtaattac gaacagttga aatttgcttc gcaaatccca tctccaacct ttaatagtcc
     6841 actggactat taaagccaat caaccactgc gctgggatgt tgacgcaaat tttgagacgc
     6901 ggtgtggggg tggaaactag cctttagttt attggagaaa acaatgaaat taaccctaca
     6961 cgaagtcgct caggtcttgg gcgcaaaaaa tgatattagt ctttatcctg atagagcatt
     7021 caacaaggtc gagttcgaca gtcgcctgat tacagaaggg gatctttttg ttcccctcaa
     7081 gggagcgcgt gacggtcatg actttattcc tgtggctttt gaaaatggct gcgtagtaac
     7141 cctgtctgaa gttagccttg atgttcctca cattctagta gaggactgct tggctgcgct
     7201 tcaacaatta gcggcttact acctagagaa aacaggggta gaagtcattg ctgtgacagg
     7261 atcaaacggt aaaacgacca ccaaggacat gattcacgat gttctggcga cgacttacaa
     7321 gacttataag acacaaggca actacaacaa cgaaatcggt ctgccataca ccgtcctcca
     7381 catgcctgat gatacagaaa aactggtctt ggaaatgggc caagaccact tgggagatat
     7441 tcatcttcta tccgaaattg ccaaaccaag tctatcagtt attacccttt ttggtgaagc
     7501 gcatttagaa tttttcggct cacgagaaga gattgccaaa ggaaaattac agattgcaga
     7561 tggaatggag ccaggtagta aactcttgat cccagcatat ccgattgcgg ataagctctt
     7621 gccgagccat gtagacctca ttcgcttcgg tgagcaggct gatttgcagg tgactagtct
     7681 ggtcgagtca aaagatagcc tgaccttcac cgtcaatttt atggacggcg acattttcct
     7741 cccggtaacg ggcaagtaca atgccaccaa tgctatggta gcaagctatg tgggcaagac
     7801 cttgggtgtg tctaatgagg ccatcaagtc agccctagcc agtctaaact tgacccgcaa
     7861 ccgtaccgag tggaagaagg cggcaaatgg agcagatatt ctcagcgatg tctacaatgc
     7921 caatccaacg gctatgcgct tgattttaga aaccttctct agtattccag ccaatgaagg
     7981 cggtaagaaa attgctgtgt tagcagacat gaaagaactt ggcagtcagt cggttgagct
     8041 tcataatcag atgattttga gcttgtcgcc tgatgtgcta gacaccgtca ttttctacgg
     8101 tcaggatatt gaaggcctgg cccagttggc tagtcagatg ttcccaatag gaaaagtcta
     8161 tttctttagg aaaaatgcag agcaggacca atttggagac ctggtgaaac aagtcaagga
     8221 agtcctcggt gggaaagacc aaatcctctt taaaggctct aattccatga atcttgggaa
     8281 agtagtggaa gcagtagaaa agtagagagg cgagacctct cttttttggc aagtcttgtc
     8341 aaaacatcga aaagttgata tactgacttt tggggaaaga gaggaattta ataagatgag
     8401 tgattttttc agcaatcaga tttctgaagc accgaacctg aacgtattgg aacatctgtt
     8461 tctcatgatt atttggtttt tcatgctcca tctttgtttt cgttgttatc aaaaaagatg
     8521 gtttcatatt gttttttggt ggcttcaatt tatacagatt gtctcccttt atacttggta
     8581 tatcgtagct gattctcctc tttcagagag tcttcctttt tatcattgtc gcttggctat
     8641 gctttttatt ctttgggcga agcccggacc tttgaaacgt tattttgcct atctcggtct
     8701 ttttggatcg cttgcggctt ttattcatcc agtatttgac ccgtttgctt ttccacacct
     8761 taccttcttt acttttgtta ttggtcacta cgccttgacg gttaattgta tgttgtacct
     8821 gttatctgat ttagaagggg agatgttgaa gggaaaagag gtagtgaaat atacgcttat
     8881 tatgaatatg ctgattctag gagtagctct tttaactggc ggaaattatg gatttttgag
     8941 acaggcacct ttagtgaata ctaataatct tcctttgaat ttcttcttgg taacctgcct
     9001 actctgtttc tctattttga gtatacaagc tatgttaatt gcctatttga aaaaagaaag
     9061 caaacaaatg aattcgcata tattgaaaaa ataagacttg aaattagttt ttcaagtctt
     9121 atttttagtt tttaaatgcg ccttgtttgt agctaatcca gaaaaagaca gggataataa
     9181 tgagcattaa tccagaatag attggcaatg tgtgactgtt tagggggtgg atttcaatca
     9241 gaccatgtag gaaatgggag ctgatgaaga aaccgaaaac ggaataaata ataagagcca
     9301 gtcgagcttt tgtcagtggc aaacaggcac gaatgacagc cagtagacct gttgaaccaa
     9361 gaacataata agagagggtt tgcatgtcat gcaagtttaa gtatccaaat acttgcatta
     9421 aatggaaaat aaggacactg agaacaacca tgagagcatt tggtagggct aagagaagag
     9481 attttctgag aaaatgtttt tctacggggc ggatatttcg ttcaaaagtt aggataaaag
     9541 gaggaaaacc ttcgacaaat tgaccaatga gggtcatttg tacttgaata aatgggaaaa
     9601 ctaacaaata ttctgcttta cctagggcaa tactagcgat acagataagg ccaagtaaga
     9661 aagagtagat tgttttgata agaaagatgg gagcaatgtg ggagatgtta ttaacaacgc
     9721 gtcgtccctc aaagagaatc tcaggaatat cgcgaaattc agaatctagg aggactagat
     9781 tagcgatttg tctagtagcc ggatcgcctt ctgccataac aatggaacaa tcagcttcgc
     9841 gtagtgcaag aatatcattg accccatcac cggtcattgc agtagtatgt ccctgtgctt
     9901 tgagggtttg gatgagcaat tttttctgat gtggggatac gcgaccgaaa atggcggttg
     9961 tttcagctct ggcaattagt tcttcgtcgt caactttgga acaatcgata tagctatcgt
    10021 agtcagcaaa tccagcttcc ttagcaatgt gtgagaccgt cacaggattg tcaccagaga
    10081 ttatttttag tgtcacttct tgcgagcgaa gataggccag tgtttctgcg gcgtcttctc
    10141 ggatagggtc agcaatttct aatagagcta gaggttcaat attctcaggt agctgatttt
    10201 gagaagaagg aagggactgt tggctaagcg caagaatcag gacacgggaa ccacgagcct
    10261 gtgcctcaga aacagaagga ggattttgtg ttagtaacat ttctggtgcc cctagaaaaa
    10321 tgcgaccaat gttttcgatt gtcattgctc cccattttct atcgctagaa aatggaatga
    10381 tgtgactagc cttatagtgg tgctctaaat caccatattc ttttcgaatg gcctgagctg
    10441 ttgaattagt atcctcactt gtctggatat aggtagcgag tatctgttgg atagtgtcaa
    10501 gtgaataatg gttggttaag gggagcaggt tttcgacggt cattttcccc tgagtaatcg
    10561 ttccggtctt atctaaacaa agaacatcaa ctcgggcaag tgtttcgacg gaatacattt
    10621 cttgtacgag gacatttttc attcccagtt tgataacagc agttaagaga gaagtgatgg
    10681 tcagcagagc aattcccttt ggtaacatac ctagtagggc agtagagcta gtaataactg
    10741 agtcttttaa gggaagaagt ttgatgaaaa atgcttctag aaaaagagca agtccgaatg
    10801 gaatgataat ttttcctgta aatttagcaa ttttatccat attgtaaaga atacgagaga
    10861 cgataggttt gtgagttttc gcctcaagca tgagcttatt ggcatagttt tccgcgccaa
    10921 catggatgac ttttgcataa atctgtccgc taactagata gctaccagac aataattcag
    10981 ccccagtatt tttcacaatc aagtcgcttt caccggttaa catggcctcg tttgcctcgg
    11041 ctataccttc taaaacaata gcatcactag gaacttgttc gccagcagac agcaagagaa
    11101 catcatctaa aacaatatct tctggtgcaa gagagagtgt cttactgtca cgaacaactc
    11161 ggataaaatc cttgttcatc aggttgagtt tgtcaatcat tcgtcgtgct cgccattctg
    11221 tcagcattcc tgaaacagcg ttgaggacaa taactccgaa gaagaaaagg ttggaccatg
    11281 cctgaacagc tagtaaggct aagaaaatgg caaaattgag tgcgttaaaa gatgtaaaca
    11341 catttcgtct gaagatttcc caattactag cactggtctt tgtcttaaaa tgattggttt
    11401 gctggttttg aatacgttct tgtacttcct tggttgataa accttttatt tgtttgttca
    11461 ttattttatc tttctttttc tagtatatag aatacatctc atacttattg taacatattt
    11521 ataatacaac agtataggtc tgtagaccta ttcttcaata gtttgaaaaa ttcccttatt
    11581 tccgatataa taagagagtt gtctagtaaa gaataaggat gaatccagtg taaatcgaat
    11641 aggataagag ctcagctctg ctatgaatca aaatacaaag aatgaaaagg aaacattatg
    11701 tcactacaag aagaaatcaa gaaacgccgc acctttgcga ttatctctca cccggacgcg
    11761 gggaagacca ctattactga gcagttgctc tactttggtg gtgaaattcg tgaagcaggt
    11821 acggtcaagg ggaaaaagac tggtaacttt gccaaatctg actggatgga tattgagaag
    11881 caacgtggta tctctgttac ctcatctgtc atgcagtttg actatgcagg caagcgggtc
    11941 aatatcctag atacaccagg gcacgaggac ttctcagaag acacttaccg taccttgatg
    12001 gcggtggatg cggctgtcat ggtggtggac tctgccaagg gtatcgaggc ccagaccaaa
    12061 aagctctttg aggttgttaa gcaccgcaac attcctgttt tcacttttat caacaagttg
    12121 gaccgtgacg gtcgtgagcc acttgattta ttgcaggagt tggaagaagt cttgggcatt
    12181 gccagctatc caatgaactg gccgattggt atggggaaat ccttcgaggg actctatgac
    12241 ctccataaca aacgtttaga actctatcga ggtgatgagc gttttgccag cttggacgaa
    12301 ggcgacaagc tctttggttc taatcccttt tatgcacagg ttttggatga tattgaactt
    12361 ctggcggaag ctgggaatga attttcagaa caggcgattt tggacggcga tttgacacct
    12421 gttttcttcg gctcagccct gacaaacttt ggtgtgcaga ccttccttga caccttcttg
    12481 gaatttgctc cagagccaca tggtcacaag acaacgacag gggatgtcat tgacccgctc
    12541 aataaagatt tttcaggctt tgtcttcaaa atccaagcca acatggaccc tcgtcaccgc
    12601 gaccgtatcg cctttgtccg tgtggtttcg ggcgaatttg aacgcggtat ggcggttaac
    12661 ctgcctcgta caggtaaggg agccaagttg tccaacgtga cgcagttcat ggccgagtcc
    12721 cgtgagaatg tggaaaatgc agtagcgggt gacattatcg gggtttatga tacagggacc
    12781 tatcaggtag gagatacctt gaccgtcgga aagaacaaat ttgagttcga accgctaccg
    12841 accttcaccc ctgaaatttt catgaaagtt tctgctaaaa acgtcatgaa acaaaaatcc
    12901 ttccacaagg gaatcgagca actggtacag gaaggggcta tccagctcta taagaactac
    12961 cagactggcg agtatatgct gggtgccgtt ggtcagctcc agtttgaagt tttcaaacac
    13021 cgcatggaag gcgagtacaa tgccgaagta gtcatgactc caatgggtaa aaagacagtc
    13081 cgctggatca agccagaaga cctggatgaa cgcatgtcat caagccgcaa catcctagcc
    13141 aaagaccgct ttgaccaacc agttttcctt tttgaaaacg acttcgccct ccgctggttc
    13201 gcggacaaat atccggatgt ggagttggaa gagaagatgt agcggaaaga cagaaatcag
    13261 taatcaacaa agttgatttg atttcgtcgt ctttccctcg caagggcgtc caaagaactc
    13321 gagcaatgcg aagagtgatt tgctgacgcc tactgcgtcg aggttcagtg aaccttttct
    13381 agtgggagat gtgaactgac ggtagtcagt tcacattgat aaagataact ataaaaggat
    13441 atgatagaat acatgaagct gggcaagacc tctatgatgt cttgcccagc ttcattttt
//
LOCUS       DASFSW010000047        13336 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.047, whole genome shotgun sequence.
ACCESSION   DASFSW010000047 DASFSW010000000
VERSION     DASFSW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13336
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.047"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            132..905
                     /gene="sufC"
                     /locus_tag="U1099_001014"
     CDS             132..905
                     /gene="sufC"
                     /locus_tag="U1099_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEM4867344.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPSYEVTQGEILFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDEDKISVRDFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGINAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAQRLEKEGYVQVAAELGIDYKEDDI"
     gene            922..2184
                     /gene="sufD"
                     /locus_tag="U1099_001015"
     CDS             922..2184
                     /gene="sufD"
                     /locus_tag="U1099_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013989976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEM4867345.1"
                     /translation="MTKEKIQEFSALQAEPTWLTDLRLKAFDKIAELDLPVIERVKFH
                     RWNLGDGSLATNDEIGAVPDFTAIGDNPMLVQVGSQTILEQLPVDLVEKGVVFTDFAS
                     AMDLIPDVIEKYLGVAVAYDEHKLAAYNTAYFNATAVLYVPDNVEIDQPVEALFYQDS
                     TSPVAFNKRVLIIAGKNAKLNYLERFESLGDGAATTSANIVVEVIALAGSQIKFAAID
                     RLGKHLDTYLNRRGYLGNDATIDWAIGLLNEGNVVADLDSELKGNGSHANLKVVGLSS
                     GRQVQGVDTRVTNYGHNSVGHILQHGVILESGTLTFNGIGHIVRGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQATAERLVVRGF
                     LGAVITEIPVKEVRDQLIAVIEEKLTKR"
     gene            2189..3412
                     /locus_tag="U1099_001016"
     CDS             2189..3412
                     /locus_tag="U1099_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM4867346.1"
                     /translation="MDFESIRKDFSILDQVVNDEPLVYLDNAATTQKPQQVLDVLEDY
                     YQTDNANVHRGVHTLSERATARYEAARQKVADFIQAKSSKEILFTRGTTTSLNWVAQF
                     AKEILQPDQEVIISVQEHHSNIIPWQQACQQIGAKLRYVPLKDGELDVDSLKSILSEK
                     TKFVSLAHVSNVLGGVVPIGEIAELVHQVGAYLVVDGAQSVPHMAVNVQELDVDFFAF
                     SGHKMLGPTGIGVLYGKEELLNLMSPVEFGGEMIDFVYEQSATWKELPWKFEAGTPNI
                     AGAIGLGAAVDYLTEIGMDAIQAHEAELVDYVFPKLQAIPGLTIYGSQDLSKRTGVIA
                     FNLDDLHPHDVATALDYEGVAVRAGHHCAQPLLRYLQVPATVRASFYIYNTKADCDKL
                     VEAIIKTKEFFNGPI"
     gene            3399..3839
                     /locus_tag="U1099_001017"
     CDS             3399..3839
                     /locus_tag="U1099_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEM4867347.1"
                     /translation="MALSRLDSLYMAVVSEHSKLPRHRGSLDGVEKLELHNPTCGDVI
                     ELSVKIEKDVITDIAFDGVGCTISTASASMMTEAVLGKEISQIQELAEIFSQMVQGQE
                     DPRQKGLGDASLLAGVAKFPQRIKCATLPWNALKKALERSDSAE"
     gene            4189..5604
                     /gene="sufB"
                     /locus_tag="U1099_001018"
     CDS             4189..5604
                     /gene="sufB"
                     /locus_tag="U1099_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEM4867348.1"
                     /translation="MSEERIEPTPIDLGEYKFGFHDENVELVASTGKGLNEEVIREMS
                     RIKGEPEWMLEFRLKSYETFKKMPMQTWGADLSELDFDDIVYYQKPSDKPARSWDDVP
                     DKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKHGIIFTDTDTALKEHP
                     ELFKKYFGKLVPPSDNKLAALNSAVWSGGTFIYVPKGVKLDIPLQTYFRINNEGTGQF
                     ERTLIIVDEGASVHYVEGCTAPTYSTASLHAAIVEIIAHEGAYMRYTTIQNWSDNVYN
                     LVTKRARAEKNATVEWIDGNLGAKTTMKYPAVYLEGEGARGTMLSIAFANKGQVQDTG
                     AKMIHNAPRTSSSIVSKSIARGGGEVNYRGQVTFAKNSAKSISHIECDTIIMDDISKS
                     DTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKEL
                     PMEYAVELNRLIAYEMEGSVG"
     gene            complement(5878..7119)
                     /locus_tag="U1099_001019"
     CDS             complement(5878..7119)
                     /locus_tag="U1099_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEM4867349.1"
                     /translation="MRKFLLTLTTVIALFFTSTPILAEDFSVAAKHAIAVEVSSGKIL
                     YEQDAETKASIASISKTLSIYLVYEALAKGEITLDTMVDISDYPYSLTSNTELSNVNL
                     DARSYSVRDLLNASLITSSNSAIIALAEKIAGSEAAFVDRMKAKVQEWGITDTKIVNV
                     SGLDNTDLGENIYPGSSPEDVNMFSALDVAIIARRLILDYPQVLDITSLNAYDFGGYT
                     YYSTNQMLSDGTHARGGVDGLKTGTSNSAGSSFLATTQQAGMRIITVVLNANDGLTSP
                     ENRFVATNDLMNYVYSNFSLVTLVKKGEAFEDSKIKVFNGEKETSPVVAAADLTVVQR
                     KQTDNVPTATFTTDIKEIDAPLKAGTLVSKLQLKDQDLIGKGYISEQASVDMIIPSDM
                     KEASWPFSWWNQFVRYVNEKL"
     gene            7332..9122
                     /locus_tag="U1099_001020"
     CDS             7332..9122
                     /locus_tag="U1099_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM4867350.1"
                     /translation="MKKTTKLFALAGVTLLSASVLAACGSKQSGAAKQELSFPAEVKQ
                     DGTAVSDAQLKYAWVSPTTSSGLLIDELTENTTDSTFGGFVDISMLGYDGERKLDDSG
                     LAKAEFDVKGKKITISLTGKDYKWSDGEPFTINDYIFTIKSMASKDYTGIRFDDKFLN
                     IEGMQEFVDGKASDISGIKKVDDYTVELTVKEMSPSMMYAGGDVPAYIQPEHIYKDIP
                     VADWEKSEYSRTAKLVGMGPWKIKEIVNGESITYVPNEYFFKGTKPKTSSLKIDIVSP
                     DTIVSEMKAGNYDIAEMPVDQLDSYKDVSNLNIVGQLESSYEYISFNLGKYDEAANKN
                     VMDEKAKMNDVKLRQAIAYAIDTKTAGKSLYNGLYHPAKSLIISFFGDIHDSELEGYS
                     YNPEKAKKLLDEAGYKDVDGDGIREGKDGKAFKITFAARKRTEANEALVQQYIAWWKE
                     VGLNVELYTGRTVEGKSFYNSVQANDPAIDMYAGGWSTGYDPNPSGLWGSVAAFNMSR
                     FVSDENTKLLEAISSAESFDDKKNVENYKAWQKYAHEQAFAIPTFESESIVALNKRVK
                     NYDTNYGSASGKGIALENIELTADKGVVAE"
     gene            9499..10497
                     /locus_tag="U1099_001021"
     CDS             9499..10497
                     /locus_tag="U1099_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867351.1"
                     /translation="MATEKPLLDIKDLHVGFRIGDDYFDAVDGVDISLQKNEILAIVG
                     ESGCGKSTLATTIMGLHNPLNTKITGSIQYNDMELIGMNEAKYNTVRGNDIGMIFQDP
                     LASLNPLMTIGAQIDEALFYHTDLDANARTERVLELLAQVGIPNPKRTFKQYPHELSG
                     GMRQRIIIAMALSCKPPIIIADEPTTALDVTIQAQILDLLNDIQAETGSGIILITHDL
                     GVVAETADRVAVMYGGQFVEVAPVEELFTNPKHPYTRSLLKSNPQSGSEGGDLHVIEG
                     IVPPITKMVRKGCRFAPRIPWIGAEAHEENPSMHEVGPNHFVRCTCHETFYFEGEA"
     gene            10500..11441
                     /locus_tag="U1099_001022"
     CDS             10500..11441
                     /locus_tag="U1099_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867352.1"
                     /translation="MGFIEVKDLKVHYPIRSGFFNRVTDHVYAVDGVNIEFEEGKTYG
                     LVGESGSGKSTIGKAIIGLERATSGQIIYEGQNVTNKSRGKKGNFNRDVQMIFQDSLS
                     SFNPKKRILDIIAEPIRNFDRLSPDEEKKKVLQLLDTIGLNEEALIKYPHEFSGGQRQ
                     RIGVARALASNPRLIIADEPVSALDLSVQAQVLNYMKRIQDEFKLSYLFISHDLGVVQ
                     HMCDELFIMYRGRFVETGNKNDIYNNPQHIYTKRLLSAIPTIDPLNRLKNKEKRLAAE
                     KEYQEMQGQYYDENGRVYDLRSFSETHQVALPEGGQN"
     gene            11443..12399
                     /locus_tag="U1099_001023"
     CDS             11443..12399
                     /locus_tag="U1099_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867353.1"
                     /translation="MWKTVLRRLLMMIPQIIILSVIAFFVAKMMPGDPFTGLIDPNID
                     PAIIEQKRIAAGYYDPIPIQYFRWVGNLLQGDFGQSVIFKQPVVDVIMQRLNNTIGLS
                     LLTMVLTYLIALPLGMIAGRYQNSLADKIIGVYNFLTFSTPTFVFAILMLWMFGFSLG
                     WFPTRGSISGGVEGFAATLSRLHHMILPAITMAILSTTVTIQYLRTGIIDAKSQDYVR
                     TARAKGVPERVVYNRHIFRNSILPIASFLGYELTGLIGGSIFIENIFTYPGIGQLFFN
                     SISSRDYSVILALLLVFGMGTLLGTLISDIIMSIVDPRIRVK"
     gene            12413..13315
                     /locus_tag="U1099_001024"
     CDS             12413..13315
                     /locus_tag="U1099_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867354.1"
                     /translation="MSKENKKQVQSASTPPGGFRVIAREFMKDRLALTSLIILVTVLL
                     AVFIGALLLNQEQVMKVNLLGRYLEPGVDGYILGTDEGGKDIFGQLIIGARNSIIIGF
                     TITIITSIVGISVGLISGYYGGRLDGFLMRIVDFIMILPVTMLIIVFVTVIKNYTIYH
                     FILIMSAFYWTAKARLFRTRTLSEASLDYVNASKTLGTSDFMIMFREILPNLSSLIIV
                     NLTLNFAGNIGIETSLTYLGFGLPSTVPSLGTLIANARNADILENKTWIWLPAAIFIL
                     VMMLCINYVGQAFQRAADAKQRLG"
BASE COUNT         3875 a         2638 c         3036 g         3787 t
ORIGIN      
        1 gggaaaacga actcttctat ggatggtcga gttctttccc cactgccaag gttgagtctt
       61 atcggtgtat ctagttagct tggtcagtta atgtggtttt tgaagaggac aaaaatgaaa
      121 aggagcacag catgtcagta ttagaaatca aagaccttca tgttgaaatc gaaggtaagg
      181 aaatcctaaa aggtgtcaac ctgaccctta aaacaggtga aattgcagcc atcatgggac
      241 cgaacggaac agggaaatcc accctttcag cagccatcat gggcaatcca agctacgaag
      301 ttacccaagg tgagattctg tttgacggtg tcaatatttt agaattagaa gtcgatgaac
      361 gtgcccgtat gggtcttttc ttggctatgc aatacccgtc agaaattcca gggattacta
      421 atgcggaatt tcttcgtgcc gcaatgaacg ctggtaagga agacgaagac aagatttctg
      481 ttcgtgattt cattactaag ttggatgaaa agatggagct ccttaacatg aaagaggaaa
      541 tggcagagcg ttacctcaac gaaggtttct caggtggtga gaaaaaacgc aatgagattc
      601 tgcaattact catgttggaa ccgacttttg cccttcttga tgagattgac tcaggattgg
      661 atattgatgc ccttaaagtc gtgtcaaagg ggatcaatgc tatgcgtggt gaaggctttg
      721 gtgccatgat tattactcac taccaacgct tgctcaacta catcactcct gatgttgtcc
      781 atgtcatgat ggaaggccgt gtcgtgcttt caggcggacc agaattggca caacgcttgg
      841 aaaaagaagg ttatgtccaa gtcgcagcag agcttggcat cgactacaaa gaagatgaca
      901 tttaagtctt tggaggaagc tatgaccaaa gaaaagattc aagaattttc agccttgcag
      961 gcagaaccaa cttggttgac agacctgcgt cttaaggctt ttgacaaaat agcagagctg
     1021 gacttgccgg ttatcgagcg ggtcaagttt caccgttgga atctaggtga cggaagccta
     1081 gctacaaatg atgaaatcgg ggcagttcca gattttacag ctatcggtga caatcctatg
     1141 ttggttcaag ttggcagcca aacgattcta gaacagttgc cggttgacct tgtagaaaaa
     1201 ggtgtggtct ttacggactt tgcctcagct atggatctta ttccagatgt tatcgagaaa
     1261 tatcttggag tagctgtagc ttatgatgaa cacaaattag ccgcctataa cacagcttat
     1321 tttaacgcaa cagctgtcct ttacgtgcca gataatgttg aaatcgacca gccagttgag
     1381 gcccttttct accaagacag caccagtcca gttgccttta acaaacgtgt cctcatcatc
     1441 gctggaaaaa atgccaagct taattatcta gaacgctttg aaagtttggg cgacggcgca
     1501 gcaacgactt cagccaatat tgtcgtggaa gtaatcgccc ttgctggtag ccaaatcaag
     1561 tttgcggcta ttgaccgtct tggcaagcat ttggatacct acctcaaccg tcgcggctat
     1621 ctcggaaacg atgcaacgat tgactgggcg attggtctgc tcaacgaagg aaatgtggtt
     1681 gccgatttgg acagtgaatt aaaaggaaac ggtagccatg ccaacctcaa agttgtcgga
     1741 ctgtcatcag gtcgtcaggt ccaaggtgtg gatacgcgcg tgaccaacta tggacataat
     1801 tcggtcggtc acatcctgca acatggggtt atcttagaaa gcggaacctt gaccttcaat
     1861 ggcatcggac acattgtccg tggtgctaaa ggtgcggatg cccagcagga aagccgtgtc
     1921 ctcatgctgt ctgacaaggc tcgctcagat gctaacccaa ttctccttat cgatgagaat
     1981 gaggtcacag ctggacacgc agcctcaatc ggtcaggtgg atccagaaga catgtactac
     2041 ctcatgagtc gtggtctgga ccaagcaact gctgaacgct tggttgttcg cggtttcctt
     2101 ggcgcagtca ttaccgaaat tcctgtcaag gaagttcgtg accaattgat tgccgtgatt
     2161 gaagaaaaac taacaaagag ataagatgat tgattttgaa agcattcgca aggatttttc
     2221 aatcctagac caagttgtta acgatgagcc gctggtctat ttggacaatg cggcgaccac
     2281 ccaaaaaccg caacaggtac tggatgtact ggaagattac taccagacag acaatgccaa
     2341 tgtccaccgt ggagttcata ctctgtcaga acgggcaacg gctcgctacg aggcggctcg
     2401 gcagaaggtg gctgatttta tccaggccaa gtctagcaag gaaattctct ttaccagagg
     2461 aacgaccacc agtctcaact gggtagctca gtttgcaaag gaaattctcc agccagacca
     2521 ggaagtgata atctcggtcc aagagcacca ctccaatatc atcccttggc agcaagcctg
     2581 tcagcaaata ggtgccaagc tccgctatgt tccactaaaa gacggcgagt tggatgtgga
     2641 tagcctcaag tccatactct ccgaaaagac caagttcgtc tccctggccc acgtatccaa
     2701 tgtcctaggt ggcgtggttc ctattgggga aatagcagag ctggtgcatc aagtcggtgc
     2761 ctacttggtg gtggacggag cccagtcagt tccccacatg gctgtcaacg tgcaagaatt
     2821 agacgtggat ttctttgcct tttcaggtca taaaatgttg ggaccgacag gaatcggcgt
     2881 tctctacggc aaggaagaat tgctcaatct catgtcgcca gttgaattcg gcggtgaaat
     2941 gattgacttt gtctatgagc agtcagctac ttggaaggaa ttgccttgga agtttgaggc
     3001 aggaacgccc aatattgcag gtgcgattgg tctgggagca gccgttgatt acctgaccga
     3061 aattggtatg gacgctatcc aggcccacga ggcagaactg gttgactatg tctttccaaa
     3121 attgcaggct attccaggct tgaccattta tggcagtcag gacctgtcca agcggacggg
     3181 agttattgcc tttaacttgg acgacttgca tccacacgat gtggcaactg ctctggacta
     3241 tgaaggtgtt gctgtgcggg caggccacca ttgtgcccaa ccgcttctca gatatttgca
     3301 ggtgcctgct actgtacgag caagttttta catttacaat accaaggctg actgtgataa
     3361 gttggtcgag gcaatcatca agacaaagga gtttttcaat ggccctatct agattagact
     3421 ctctttatat ggcagttgtg tctgaacact ccaagttgcc tcgtcaccgc ggaagtttgg
     3481 acggagtgga aaagttagaa ctccacaatc caacctgcgg tgatgtgatt gaactatcag
     3541 tcaagattga aaaagatgtg attaccgata ttgcttttga cggcgttggc tgcaccattt
     3601 caaccgcatc agcttctatg atgactgaag cagtgcttgg caaagaaatc agtcagattc
     3661 aagaactagc agaaatcttc tcacaaatgg tccaaggtca agaagaccca cgccagaagg
     3721 gactgggaga cgcctccctc ctcgcaggtg ttgccaaatt cccccaacgc atcaaatgtg
     3781 ccacattacc gtggaatgcc ctgaagaaag cactggaacg aagtgatagt gctgaatgaa
     3841 gggtaactgc gacatgagcg attttggtcg atattaccaa aatcagccaa ttagtaagcc
     3901 acttagcaag gttgattaga accttgcgtt acttggttag gaactttagt tccaaccaag
     3961 tttagtgtct tgaagaaagc aatcgaacga agtgaagatg cttactgagc atcatcgaat
     4021 aaacaaaaat gaaaatcata cagaaaggat tgttcagttt ctgattggaa atgaaattga
     4081 actcgcccta aaagcttttg aaaaaagata aatctccgcg tgtgtttgcg cacgaagtcg
     4141 atttcctgtt tttcatttgc tttcttaagg ggcttagtat cttatattat gtcagaagaa
     4201 agaattgaac caaccccgat tgatcttggg gaatacaaat tcggtttcca tgatgaaaac
     4261 gtggaactag tggcttcaac tggtaagggt ttgaacgaag aagtcattcg tgaaatgtcc
     4321 cgtatcaaag gcgagcctga atggatgttg gaattccgtt tgaaatccta cgaaaccttc
     4381 aagaaaatgc cgatgcagac ctggggtgct gatctgtctg aactggattt tgatgacatt
     4441 gtttactatc aaaaaccgtc agataagcca gcacgcagct gggatgatgt accagacaaa
     4501 atcaaggaaa cctttgaacg catcggtatc ccagaagctg agcgtgctta cctggcgggt
     4561 gcttctgccc agtacgaatc agaagtggtc taccacaata tgaaagaaga gtacgacaag
     4621 cacggcatta tctttacgga taccgacacg gctcttaaag aacacccaga gcttttcaaa
     4681 aaatactttg gaaaactcgt tccgccgtca gataacaaac tggctgccct caactcagcg
     4741 gtttggtcag ggggcacctt tatctacgta ccaaaaggtg tcaagctgga tattccgctt
     4801 caaacctact tccgtatcaa caatgaagga acagggcagt ttgaacgcac cctcatcatc
     4861 gtagatgaag gagcaagcgt tcactacgta gaaggctgta cagctccgac ctactcaaca
     4921 gcaagtctcc atgcagcgat tgtagaaatc attgcccacg agggtgccta tatgcgttat
     4981 accaccattc aaaactggtc tgacaacgtc tataacttgg taaccaaacg tgcccgtgcc
     5041 gagaaaaatg ccacagttga gtggattgac ggaaaccttg gtgctaagac gaccatgaaa
     5101 taccctgcgg tttacttgga aggtgaagga gcccgtggaa ccatgttgtc cattgccttt
     5161 gccaataaag gtcaagtcca ggataccggt gctaagatga ttcacaatgc cccacgtacc
     5221 tcatcttcta tcgtgtccaa atccattgcc cgtggcggtg gagaagtcaa ctaccgtggt
     5281 caagtgacct ttgctaaaaa ctcagccaag tcgattagcc acatcgagtg tgatactatt
     5341 attatggatg atatttccaa gtcagacacc attccattta atgaaatcca caactcacaa
     5401 gtggccctcg aacacgaagc caaagtctca aaaatctcag aagaacagct ctactacctc
     5461 atgagccgtg gcctatccga atccgaagcc acagagatga ttgtcatggg ctttgtcgaa
     5521 ccattcacca aagaactccc aatggaatac gcagtcgaac tcaacagact gattgcctat
     5581 gagatggagg gttcagtggg atagtttgcc gcgaacttga acagctttaa agctgttcaa
     5641 gcccgtcaaa ctccccactg aagtaggaga tgaggagcga cggcagtcgc tcttctttat
     5701 aaataacata gtctgggaga ctagattacc ctactgacat cgcttcctgt aaggaagtga
     5761 tatgtaataa agagttctgc aggaactctc accatccgag gctggagata aggagtagcg
     5821 gttgtcactc tacgataaaa aaatagtatg tcgaaaatcg ttcgacatac tattttttta
     5881 taatttttca ttgacatagc ggacaaattg attccaccaa gagaatggcc aggatgcttc
     5941 tttcatatcg ctagggataa tcatatcgac actagcttgc tcggaaatat aaccttttcc
     6001 aatgagatct tgatctttca gttgtagttt gctgaccaag gtccctgctt tgaggggagc
     6061 atcaatttct tttatatccg ttgtaaaagt tgcggtgggg acattatcag tttgttttcg
     6121 ttgaacaacg gttaaatctg ctgctgcaac aacaggactt gtttcttttt cgccattgaa
     6181 aacctttatc ttactatctt caaaggcttc accttttttt actagtgtaa caagcgaaaa
     6241 gttagaataa acatagttca tcaaatcatt tgtcgcgaca aagcgatttt ctggagaggt
     6301 cagcccatca ttggcattca atactactgt gataatgcgc ataccagctt gttgagttgt
     6361 tgcaagaaaa cttgaaccag ccgagtttga ggtccctgtt tttaagccgt ccacccctcc
     6421 acgagcgtga gtgccgtcgc tcagcatttg gttggtacta tagtaagtgt agccgccaaa
     6481 atcgtaggcg tttagcgagg taatgtcgag tacctgagga tagtcgagta ttaatcgtct
     6541 tgcgatgata gcaacatcta gggccgaaaa catatttaca tcttcaggac tggaacctgg
     6601 ataaatattt tcacccaagt cggtattatc tagcccggat acattgacaa tttttgtatc
     6661 ggtaattccc cattcttgga cttttgcctt cattcgatcc acgaatgcag cttcgctacc
     6721 ggctattttt tctgccagag caataatagc actattggat gatgtgatta aagaagcgtt
     6781 taataggtct cttactgaat aggaccgtgc atccaggttt acattactta attctgtatt
     6841 agaagtgagg ctatagggat aatcagaaat atctaccata gtatctaaag tgatctcacc
     6901 ctttgccagt gcttcgtaga ccaggtaaat gctcaatgtc ttactgatag aagcgatgct
     6961 tgctttggtt tcggcatctt gctcatacaa gattttgccg ctacttactt cgactgcgat
     7021 agcatgcttg gctgcaacag aaaaatcttc tgccaaaata ggtgtggaag tgaaaaaaag
     7081 agcaataact gtagtgagtg ttagtaaaaa tttacgcatg atcattcctt tggctgttat
     7141 tttattctat tatatcacaa gaaatgtgag aaaaatttgg aaaaaacatg taaaaaatga
     7201 gaaaatgatg aaaaaatatg tttttacaaa attttctgac aaaaagactt tatttaagaa
     7261 aaatttatga tataatgatt tttataagac atagtaataa gtgtcaaaca aaaagaaaac
     7321 agaggtgttt tatgaaaaag acaacgaaac tttttgcact tgcaggtgtg actctccttt
     7381 cagcatctgt tcttgcagca tgtggttcaa aacaatcagg tgcagcaaaa caggaattgt
     7441 cattcccagc tgaagtaaaa caagacggaa cagcagtgtc tgacgctcag ttgaaatacg
     7501 catgggtatc accaacaact tcatcaggtc tcttgattga tgagttgact gaaaatacga
     7561 ctgactcaac atttggtggt tttgttgata tttcaatgct cggttacgac ggagaacgta
     7621 agttggacga ctcaggtctt gccaaggctg aatttgatgt gaaaggtaaa aaaatcacaa
     7681 ttagcttgac aggcaaggat tacaagtggt cagatggtga gccattcaca atcaacgact
     7741 acatctttac aattaagtca atggcaagca aagactatac aggtattcgt tttgatgata
     7801 aattcttgaa cattgaaggc atgcaagaat ttgttgatgg aaaagcatca gacatctctg
     7861 gtattaagaa agttgatgac tatacagtag aattgactgt aaaagaaatg tcaccttcaa
     7921 tgatgtatgc aggtggtgat gtcccagcct atatccaacc agaacacatc tataaagaca
     7981 tccctgttgc agactgggaa aagagcgaat actcacgtac tgcgaagctt gttggtatgg
     8041 gaccgtggaa gattaaagaa attgttaatg gtgagtctat cacttacgta ccaaacgaat
     8101 actttttcaa gggtacgaaa ccaaaaacaa gctcattgaa gattgacatc gtatctcctg
     8161 atactattgt ttctgaaatg aaagcgggga attatgatat tgctgaaatg ccggttgatc
     8221 agttagattc atataaggat gtttctaact tgaatatcgt tggtcagtta gagtcgtctt
     8281 atgagtatat ctcgtttaat cttggtaaat atgacgaagc agctaataag aacgttatgg
     8341 atgaaaaggc taagatgaat gatgtgaaac ttcgtcaagc aattgcctat gcaattgata
     8401 ccaagacagc tggcaaatca ttgtacaacg gtctttacca ccctgcaaaa tcattgatta
     8461 tctcattctt cggggatatt catgattcag agttggaagg ctattcatac aatcctgaaa
     8521 aagcgaaaaa gcttttggat gaagctggct acaaagatgt agatggcgat ggtatccgtg
     8581 aaggtaaaga cggtaaggcg ttcaaaatta cctttgctgc tcgtaaacgt actgaagcta
     8641 acgaagcgct tgtacaacaa tatattgctt ggtggaaaga agttggcttg aatgtcgaac
     8701 tttacacagg acgtacagtt gaaggtaagt cgttctacaa ctcagttcaa gctaatgatc
     8761 ctgctattga tatgtatgct ggtggatggt caacaggtta cgatccaaat ccaagtggtc
     8821 tatgggggtc tgtcgcagcc ttcaatatgt cacgtttcgt gtccgatgaa aacaccaagt
     8881 tattggaagc tatcagctca gcagagtcgt ttgatgataa gaaaaacgtt gagaactata
     8941 aggcttggca aaaatatgct cacgagcaag catttgctat tccaacattt gaatctgagt
     9001 caattgtagc acttaacaaa cgtgtgaaaa actatgacac taactacggt tcagcttcag
     9061 gtaaaggtat cgctcttgag aatatcgaac ttactgctga taaaggtgta gtggcagaat
     9121 aatttgttag aagacaatag aatctgaaaa agccaatttg attggctttt ttcagattgc
     9181 tattggaatt ttcagataat tgtggtatag tagactcagt acacaaaaat gagtccctac
     9241 taacaaaaac tcaagatgaa gaacattaac ctactcaatt cctaattgag acagtaatag
     9301 ggatggtctt gatttatgat tggatatagg tacatttttt acttctgggt aatcgctatc
     9361 aaacgataat ttgatagaaa tatgttattc tagagtagtt atataaagtc agttaatcaa
     9421 acgttatatg aacttgataa tcgtaaggct attgaattga ttgagcttga ttagctggca
     9481 cataaaggag gaaaaagcgt ggctaccgaa aaaccgcttt tagacattaa agatttacac
     9541 gtcggtttcc gtattggaga tgattatttt gacgctgttg acggtgttga catctcattg
     9601 caaaaaaatg aaattttggc aattgtaggg gaatctggct gtggtaaatc aactttggca
     9661 acgaccatca tgggcttgca taaccctttg aataccaaaa tcacaggaag catccagtac
     9721 aatgatatgg aattgattgg catgaatgaa gctaagtaca atacagtacg cggtaatgat
     9781 attggtatga ttttccaaga tcccttggct tctctaaatc cattgatgac gattggtgca
     9841 cagattgatg aggcgctgtt ctatcacact gatttggatg ccaatgctcg tacagagcga
     9901 gttttggaac tcttggctca ggtaggtatt ccaaatccta agcggacatt taaacagtat
     9961 ccacatgagc tttcaggtgg tatgcgtcag cgtatcatta ttgcgatggc cttgtcttgt
    10021 aaaccgccaa ttattattgc ggacgaacct acaacagcct tggatgttac aattcaagca
    10081 caaatcttgg atctcttaaa tgatattcaa gcggagacag gttctggtat tattttgatt
    10141 acccatgact tgggagtagt tgcagaaact gctgatcgtg tggctgtcat gtacggtggt
    10201 cagtttgtcg aagtagcgcc agtagaagaa ctctttacca atccaaaaca tccatataca
    10261 cgttcgctct tgaagtcaaa tcctcaatca ggtagcgaag gaggcgatct tcatgttatt
    10321 gaaggtattg tgccgccgat tacaaaaatg gttcgtaaag gttgccgttt tgctccgcgt
    10381 attccgtgga ttggagcaga ggctcatgaa gagaatccga gcatgcacga ggtaggtcca
    10441 aatcactttg ttcgttgtac ctgccatgag acattctatt ttgaagggga ggcctaatag
    10501 tgggatttat tgaagtaaaa gatttaaaag tccattatcc aattcgtagt ggcttcttta
    10561 accgtgtgac ggaccatgtc tatgctgtgg atggtgttaa tattgagttt gaagaaggaa
    10621 aaacctatgg tttggtaggt gagtcaggtt ctgggaaatc aaccattggt aaggcaatta
    10681 tcggtttaga gcgtgccact tctggtcaga ttatctatga aggacaaaat gtaaccaaca
    10741 aatcacgtgg taaaaaagga aactttaatc gtgatgttca aatgattttc caagattctc
    10801 tttcaagttt taacccgaaa aaacgtattt tggacatcat tgcagaacca attcgtaact
    10861 ttgaccgttt atctccagat gaagaaaaga aaaaagttct tcaactcttg gatacaatcg
    10921 gtctaaatga agaagctctg attaagtatc ctcacgaatt ctcaggtggt caacgccaac
    10981 gtatcggtgt tgctcgtgct ttggctagta atcctcgttt gattattgca gatgagccgg
    11041 tttctgcctt ggatttgtct gtgcaggcac aggtcttgaa ttatatgaaa cgtatccaag
    11101 atgagtttaa gctcagctat ctctttattt cccatgacct cggtgttgtg caacacatgt
    11161 gtgatgaatt gttcatcatg tatcgcggac gtttcgtaga aacaggaaat aagaatgata
    11221 tttataataa tccacaacac atttatacta aacgtttgtt gtcagctatt ccaacgattg
    11281 acccactcaa tcgattgaag aataaagaaa agcgtttggc tgctgaaaaa gaatatcaag
    11341 aaatgcaagg tcaatactat gatgaaaacg gtcgtgtgta cgatttgagg tccttctcag
    11401 aaactcatca agtagccctt ccagaaggag gtcagaacta agatgtggaa aacagtatta
    11461 cgtcgtttac tcatgatgat tccgcagatt attattttga gtgtcattgc gttctttgtt
    11521 gcgaagatga tgccggggga tccctttaca ggattgattg accccaatat tgatccagca
    11581 atcattgaac aaaaacggat tgcggcaggt tactatgacc caatccctat tcagtatttc
    11641 cgctgggtag gcaatctttt gcaaggagat tttggtcaga gtgttatttt taaacaacca
    11701 gttgttgatg tcatcatgca gcgcttgaat aatacaattg gattatccct cttgacgatg
    11761 gtattgactt atttgattgc acttccattg gggatgattg caggtcgtta tcaaaactca
    11821 cttgcagata aaattattgg cgtgtataac ttcttgacat tttcaactcc aactttcgtt
    11881 tttgccatcc tcatgctctg gatgtttggc tttagtttgg gctggttccc gacgcgcggt
    11941 tcgattagtg gtggtgtgga aggatttgca gcgactctca gtcgtttaca ccatatgatt
    12001 ctgccagcga ttacaatggc tattttgtca acaacagtta ctattcaata tctccgtacg
    12061 gggattattg atgctaagag tcaggactat gttcgtacag ctcgtgccaa aggtgttcct
    12121 gagagagttg tttataaccg tcatatcttc cggaattcta ttttgccaat tgcatctttc
    12181 ttgggctatg aattgacagg attaattggt ggttctatct ttattgaaaa tattttcact
    12241 tacccaggta tcggtcagtt attctttaac tctatttcta gtcgtgacta tagtgttatc
    12301 ttagctttat tgttggtatt tggtatgggt actttgttgg ggacattgat ttcagatatt
    12361 atcatgagta tcgtggatcc acgtatccgt gtgaaataga aggaggagta aggtgagtaa
    12421 agaaaataaa aaacaagtac aatctgcttc aactcctcca ggtggcttcc gtgtcatcgc
    12481 gcgggagttt atgaaagatc gacttgcatt gacctcatta attatcttag tgacggttct
    12541 cttagcagtc tttatcggtg cacttctgtt gaatcaagaa caagttatga aagtcaacct
    12601 tttgggacgt tacttggaac caggtgttga cggctatatc ttggggacag atgaaggtgg
    12661 taaagatatc tttggtcagc tgattatcgg cgctcgtaac tcaatcatta tcggttttac
    12721 cattacgatt attacaagta ttgtcgggat ttctgtcggt ctgatttctg gttattatgg
    12781 cggtcgtttg gacggattct tgatgcgtat tgtcgatttc atcatgattt tgccggtgac
    12841 aatgttgatt attgttttcg tgacagttat caagaactat acgatctatc atttcattct
    12901 catcatgagt gccttttatt ggacggccaa ggctcgtcta ttccgtacaa ggactttgtc
    12961 agaggcaagt ttggactatg taaatgcatc aaaaacattg ggaacaagcg actttatgat
    13021 tatgttccgt gaaattttac caaacttaag ttcattgatt atcgttaact tgaccttgaa
    13081 ctttgcggga aatatcggta tcgagacttc gttgacttat cttggtttcg gtctgccatc
    13141 gacagtacca agtttgggta ccttgattgc caatgcccgc aatgcagata ttttggaaaa
    13201 caagacatgg atttggttgc cagcagccat cttcatcctt gtgatgatgc tctgtatcaa
    13261 ctatgtcgga caagctttcc aacgtgcagc agatgctaag cagcgtttag gttaattgtg
    13321 aaaagggttc ctttgg
//
LOCUS       DASFSW010000048        13309 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.048, whole genome shotgun sequence.
ACCESSION   DASFSW010000048 DASFSW010000000
VERSION     DASFSW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.048"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(178..1530)
                     /gene="rlmD"
                     /locus_tag="U1099_001025"
     CDS             complement(178..1530)
                     /gene="rlmD"
                     /locus_tag="U1099_001025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEM4867355.1"
                     /translation="MNLQVNQRIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEEIFC
                     QITSVKRNFVEAKLLKINKESKFRVKPACDIYQACGGCQIMHLRYDKQLDFKQDLLQQ
                     ALKKFKPAGYENYTIYPTIGMDKPSHYRAKLQFQTRSFKDQVKAGLYAENSHRLIGIT
                     NCYVQDAETQEIINGIAKLLTKHRIPIYNERKQHGIRTVMVRKARKTGQVQVIFITSC
                     QVNLIKLIDELTSQFPSIITIALNWNRQKTSDIYGEKTEILWGEQAIDEAVLDYEFSL
                     SPRAFYQLNPQQTEILYGEAVKALDISGDEDLIDAYCGVGTIGFAFAKKVKSVRGMDI
                     IPEAIEDAKANAKRMGLSNTHYEAGKAEEIIPKWYQEGYRADALVVDPPRTGLDEKLL
                     KTILDYKPAKMVYVSCNVSTLARDLVELSKAYKVEYIQSVDMFPHTARTEAVVKLSKK
                     "
     gene            1568..2344
                     /gene="recX"
                     /locus_tag="U1099_001026"
     CDS             1568..2344
                     /gene="recX"
                     /locus_tag="U1099_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEM4867356.1"
                     /translation="MRITKIEKKKRLYLLEVDGQDSLYITEDTIVRFMLSKGKEITEQ
                     EFRELRDFAQFSYGKNLALYYLSFKQRTKKEVSDYLKKYEIEENNIVKIVMVLEEEKW
                     LDDGNYVDSYVRQNALNGDKGPAMIRQKLMQKGIPKPLIDKRLAEEDFSELAGKIGEK
                     LVGRYQRKLPLRALQDKVVQGLMGKGFSYETAKQSLSQLELVADEENEDDLIAKELDK
                     QYRKYSRKYEGYELKQRLIQALARKGYDFDRIQAVLRDYL"
     gene            2420..2953
                     /locus_tag="U1099_001027"
     CDS             2420..2953
                     /locus_tag="U1099_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEM4867357.1"
                     /translation="MKLPKEGDFITIQSYKHDGEIHRTWRDTMVLKTTENAIIGVNNH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRENGVSYYCNLASPYVLDNEALKYI
                     DYDLDVKVFADGEKRLLDVDEYERHRKAMKYSDDIDFILKENVKILVDWINNQRGPFS
                     PAYVNIWYKRYLELRSR"
     gene            3011..3328
                     /locus_tag="U1099_001028"
     CDS             3011..3328
                     /locus_tag="U1099_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEM4867358.1"
                     /translation="MAFEQTNGRYASFGVVTSLPGEVIDSFWYVIDHYLKGVIPLKSV
                     IRFSIKNRRGKITLVFSQEGYKNVLAVDLSSRFDPFYPSTILVMDKQGKETITLPDEV
                     TLL"
     gene            3428..4159
                     /locus_tag="U1099_001029"
     CDS             3428..4159
                     /locus_tag="U1099_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM4867359.1"
                     /translation="MVSQTVEKPLYLQLVDELEVAIRERMAPNDKLFSERELTQVYGV
                     SRITVRLALQELEKRGLVYKKHGKGTYVSEISDTAVDLSQAYSFTEQMKKIGKVPRTS
                     ILSFELVKASDYIAQHLQLSQGEEVFEVERLRLADEVPMMLERTYVPASLFPGLAAQR
                     MKSTPLYEIFSEDYHQVIRLAEEEFYASIALDNEAKILGIPSNSPVLHLVRKTYNDKN
                     RIIEFTFSIARADQFRYKITHQRGY"
     gene            complement(4299..5009)
                     /locus_tag="U1099_001030"
     CDS             complement(4299..5009)
                     /locus_tag="U1099_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM4867360.1"
                     /translation="MKVPKYQLIQNDLRQQIVSGKFENGDKFYTESELTKLFNVSSIT
                     VIRAVNELVKDGYLVRQQGKGTFVSRSRKGRLVEFSDIEIFPMDKDKVTVLSCEKGNK
                     PDILEKLNLDKNEFYYKIVRVRAAEDTPYIFHNSYIPQRYIQNPDAPLEHYQSIYQRF
                     KLDYNIHMSEEPFVETNEIVSPCPKEVATHLKLKATEPAVLQNKTTTNSTSGEVMEYT
                     ETYKHWKYYKFEITANHR"
     gene            5216..6988
                     /locus_tag="U1099_001031"
     CDS             5216..6988
                     /locus_tag="U1099_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEM4867361.1"
                     /translation="MKEFYIGDQFYLDGEPFKILSGAIHYFRVHPDDWYHSLYNLKAL
                     GFNTVETYVPWNMHEPRKGEFCYEGILDIERFLKLAQELGLYAIVRPSPYICAEWEWG
                     GLPAWLMKEELRVRSSDSVYLQHLDEYYASLIPKLAKLQLAQGGNVLMFQVENEYGSY
                     GEEKEYLRSVAGLMRKHGLTAPLFTSDGSWRATLRAGTLIEDDVFVTGNFGSKARENF
                     ANMTAFFNEHQKNWPLMCMEFWDGWFNRWGDEIIRREPEEMVDSVMECIELGSLNLYM
                     FHGGTNFGFMNGCSARGQIDLPQVTSYDYDAILDEAGNPTKKFYILQQRLKEVYPELE
                     YAEPLVKEAKAFSDVSLHDKVSLFATLENVSGCVKGFYPKNMEELDQSTGYILYRTEL
                     ERDKTEAERFRVVDARDRIQIYADGKFVATQYQTEIGDDVELDFKDDKLTLDILVENM
                     GRVNYGHKLTAPTQSKGLGRGAMADLHFIGHWETYPLPLDSVEDLDFSKGWEEGQAAF
                     YRYQFELDELADTYLDMTGFGKGVVFVNNVNIGRFWEKGPILYLYIPKGYLKKGANEI
                     VVFETEGKYREKIHFSQRPVIKDL"
     gene            7042..7527
                     /locus_tag="U1099_001032"
     CDS             7042..7527
                     /locus_tag="U1099_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEM4867362.1"
                     /translation="MAIIATRIDGRLIHGQVANLWTTKLNIGRIMVIDDAVAQNDIEK
                     QGLKLACPPGVKLSILPIEKAANNIKEGKYDSQRLLIVARRPENFLRLVEYGVELPEL
                     NVGNMSQTPETRSVTRSINVVDKDIADFDALAAKGVKLFAQMVPGDSPKDFMPLLDKV
                     R"
     gene            7566..8468
                     /locus_tag="U1099_001033"
     CDS             7566..8468
                     /locus_tag="U1099_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEM4867363.1"
                     /translation="MMQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGN
                     MEVGLLIGASLQLVVLGVGTFGGASRIDATSGAVLATAFSISQGLDPEIAISTIGVPV
                     AGLLVYTDILGRFTTTFFAHRVDAAIERFDYAGIERNYLLGAIPWALSRALPVFLALA
                     FGGGIVETLVTTIELPEYKWIAAGLTLAARMLPGLGFAILLHYLPLKRNLHYLAVGFA
                     LTAMLTVLYGNVSALGGAVAGIVGTLPEDAGVSFVNNFKGLSMIGIAIVGAFLSVIHF
                     KNSQKVTVVAPSNSESGEIEDDEI"
     gene            8455..9273
                     /locus_tag="U1099_001034"
     CDS             8455..9273
                     /locus_tag="U1099_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM4867364.1"
                     /translation="MTKSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKLHTQFFNTSPFFHTIITGIDLALEESDGVASKDAVNGVKT
                     GLMGPFAPIGDSIFGSLVPAIMGTVAATMGKEGSPVGIFLWVAVAIAYDIFRWKQLEV
                     AYKEGTKLITTMRDRLTALVDAASVMGVFMMGALIATMINFEVTWAPAIGEKVIDIQD
                     ILNTIFPRLVPAIFTGFVFWLLGRRGMTSTKAILIIIVLALGLSAIGHFLFGMV"
     gene            9273..9674
                     /locus_tag="U1099_001035"
     CDS             9273..9674
                     /locus_tag="U1099_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM4867365.1"
                     /translation="MVRSLVLVSHGIFCEELKKSTEMIMGPQEDIYTVALLPEEGPED
                     FQKKFEETIAGLEDFVVFADLLGGTPANVVSRKLIEGGQFDLYAGMNMPMVIGFLNGV
                     LLGEAVDYVEFGTSNLVHVNSLLTSDEDDDE"
     gene            9890..10891
                     /locus_tag="U1099_001036"
     CDS             9890..10891
                     /locus_tag="U1099_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM4867366.1"
                     /translation="MIEVKTFGGQAKLYCLENKNGMQVTLTDFGARVVEVLLPVEENG
                     GLRNVSLAAKSDDDYRKTDLYPGSSIVPVAGRISGAQAEIKGTSYQFTENEPGRTLHG
                     GIDTANEQYWDVALDHEKNQVTFGIVLKDGFNGFPGDVRVEAIYGLTDQNELTIDYRA
                     VSSKDTIFNPTNHIYFNLTGDFQQSVAEHEIRIAANRYAPLGEDNLPTGVLEDVTGTP
                     FDFRERARFAQGFDSQHPQNILVKGYDHPWLLEQVEVPIEVVSPDGKISLRVRTNQAS
                     VVIYTYNYPVEALAAYHGAFSLECQALPNACNLGGFGSILLEKDEEFLSQTVYQFTW"
     gene            10996..11253
                     /locus_tag="U1099_001037"
     CDS             10996..11253
                     /locus_tag="U1099_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEM4867367.1"
                     /translation="MMTKEQEFLKEFEAWVNTQVMVNEMAVEESRRVLEEDKDERAAD
                     AYIRYESKLDAYRFIQGKFANYHAGKGFHDLPDELFGQRHY"
     gene            11268..11654
                     /locus_tag="U1099_001038"
     CDS             11268..11654
                     /locus_tag="U1099_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEM4867368.1"
                     /translation="MAKKKVNRKKQLKKQLADLKRAGRVGLERAAEVVETVAHKAEAV
                     VEHAVEQVKEVVAEVTSSATSLEDFLALPELEGIAAARLETFYEAGIQSVADFANHTE
                     KELLALKGIGPATIKQLKEKGIELKA"
     gene            11896..12285
                     /locus_tag="U1099_001039"
     CDS             11896..12285
                     /locus_tag="U1099_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM4867369.1"
                     /translation="MLHHVEIYVSNLETSRAFYDFLLTKLGYSLYQEWEEGLSYKKAE
                     QYLVFVQTPQDFLKAGYHRCRTGLNHLAFHVGTPDDVDQWRKEFLTRRVKLLYNDRYP
                     YAGGPDHYALYLEDPDGIKIELVGEVT"
     gene            12282..12857
                     /locus_tag="U1099_001040"
     CDS             12282..12857
                     /locus_tag="U1099_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEM4867370.1"
                     /translation="MNLVIIGAQASGKMTIGQEVAKLTGMTLFHNHDSIDFSLRFIPE
                     FSEDMFDLNTRITFAVYDVFARSGRPLIGTALINFKNLIEVQFLTTVQTIFHYHGQEI
                     LFVELETALEERLRRNRTENRLTHKPLKRHIEVSEAEILSTADTCQYTSLGIPEGIQH
                     YLKINNTHLTANEVAQLIIKKMEELEQKQTK"
     gene            13021..>13309
                     /locus_tag="U1099_001041"
     CDS             13021..>13309
                     /locus_tag="U1099_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867371.1"
                     /translation="MKMLNKFTENSKDLRNELTSWFVIISLAFLLFLSIPWLVQKTLY
                     FTLSKPFEAQRVAITKSNKTSDTASGVEGTYINNIPFNDFEKLMNFSNVSFK"
BASE COUNT         3841 a         2236 c         3099 g         4133 t
ORIGIN      
        1 agctgttttt ttctcaaaaa ataacaaaat caaaatgctc catatctact ttgagtggta
       61 ggtggctatt tttgatttta aatgggtatc agtgtaggtc gtaggggact tttttctaca
      121 ctcaaaaagg ctctatatga ccatagtggg tttacccgtc atagtcttat aggcctttta
      181 ttttttgctt agtttaacaa ccgcctcggt ccgtgctgtg tgaggaaaca tgtcgacaga
      241 ttgaatgtat tctaccttgt aggctttgct tagctcaaca aggtcccttg caagagtgga
      301 gacgttgcag gatacataaa ccattttagc tggtttgtag tccaaaatgg ttttgaggag
      361 cttttcatcg agtccagttc gtggaggatc gactaccaag gcatcggccc tgtagccttc
      421 ttggtaccac tttggaatga tttcttctgc tttacctgcc tcatagtggg tattggatag
      481 tcccattcgc ttagcgtttg ctttagcatc ctcaatggct tctggaataa tgtccatacc
      541 tcgaacagat ttgacctttt tagcaaaggc gaagccgatt gtgcccacgc cacagtaggc
      601 atcaatcaag tcttcatctc ctgaaatatc caaagccttg acggcctcgc catagagaat
      661 ttctgtttgt tggggattta attggtaaaa tgcccgtgga gatagtgaga attcgtaatc
      721 taaaacagct tcatcgatag cttgttctcc ccataaaatt tctgtttttt caccataaat
      781 atcactcgtt ttctgtctat tccagtttag agcaatagtg attatacttg gaaattgact
      841 ggtgagttca tcaattaatt taataagatt aacctgacaa gaggtgataa agatcacttg
      901 aacttgacct gtttttcgag ccttgcggac cataaccgtg cggattccgt gttgcttgcg
      961 ctcattatag ataggaatac ggtgcttggt gagtagcttt gcgattccgt tgataatctc
     1021 ttgtgtttcg gcatcttgga cgtaacaatt cgtaattcca atcaagcgat gggaattttc
     1081 cgcatagaga ccggccttga cttgatcttt gaaagatcga gtctggaact gtaatttggc
     1141 acgatagtgg ctaggcttat ccataccgat tgttggataa atagtgtagt tttcgtagcc
     1201 agctggtttg aactttttta gggcctgctg caagagatct tgtttgaagt ctaattgttt
     1261 atcatagcgg aggtgcatga tttgacaacc accacaggct tgatagatgt cacaggccgg
     1321 cttgacacgg aattttgatt ccttgttgat ttttaacaat tttgcttcaa cgaaatttcg
     1381 tttgactgag gtaatttgac aaaaaatttc ttctccctta agggcaccag ggacaaagac
     1441 tagcgttttt tgataaaatc cgatgccttc tccgttgatg cccattcgtt tgatttttaa
     1501 ggggattcgt tggtttactt gtagattcat attattattt taccgaaaaa tcctgtataa
     1561 tgaaagaatg agaattacaa aaattgaaaa aaagaaacgt ctttatttac ttgaagtgga
     1621 tggtcaggat agtttatata ttacggaaga cacaattgtt cgcttcatgc ttagtaaggg
     1681 aaaggaaatc acggagcagg agtttcgtga gcttcgcgat tttgctcagt tttcttatgg
     1741 gaaaaatcta gcactttatt acctttcctt caagcaacgt accaaaaaag aagttagtga
     1801 ttatctaaaa aaatatgaaa ttgaagaaaa caatattgtc aagattgtga tggtattaga
     1861 agaggaaaaa tggctggacg atgggaatta tgttgatagc tatgtccgtc agaatgctct
     1921 aaatggagat aagggaccag ctatgattcg tcagaagttg atgcagaaag gaattcctaa
     1981 accgctgatt gataaaaggc tggcagagga agatttttca gagcttgctg ggaagattgg
     2041 ggagaaattg gtgggtagat accaaagaaa attgcctctt cgagctttgc aagataaagt
     2101 tgtacaggga ttgatgggga agggtttttc ttatgagacg gccaaacaaa gcctgagtca
     2161 gttggaactt gtggctgatg aggaaaatga agatgactta attgccaagg aattggataa
     2221 gcagtataga aaatatagtc gtaaatatga aggctacgaa ttgaagcaaa ggttgataca
     2281 ggctttagct cgaaagggtt atgactttga tcggattcag gctgtccttc gtgattatct
     2341 gtgaaaaaat ttagcctagg ttagaaaaat atgatataat agtcaagata aattgtaatt
     2401 gtagaaagtt ggtaacaaag tgaaattacc aaaggaaggc gactttatta caattcaaag
     2461 ttataagcat gacggtgaaa ttcatcgcac ttggcgtgac accatggtat taaaaacaac
     2521 ggaaaatgct attattggag tcaataatca taccttagtg actgaaaatg atggtaggcg
     2581 ttgggtaaca agagaaccag caatcgttta ttttcataaa aaatactggt tcaatatcat
     2641 tgcaatgatt cgtgaaaacg gagtttccta ttattgtaac cttgctagtc catatgttct
     2701 cgacaatgag gcactcaaat acattgatta tgacctagat gttaaggtct ttgctgatgg
     2761 tgaaaagaga ctgctggatg tggatgaata tgagcgtcat cgtaaggcga tgaagtattc
     2821 ggatgatatt gattttattt tgaaggaaaa tgtcaagata ctggttgatt ggattaataa
     2881 ccagcgcgga ccattctctc ctgcttacgt taatatctgg tacaaacgct atttagaact
     2941 gagaagtaga taaagttaga aaagaaaatc ctgttttaca ggcttttttc ttattgtaaa
     3001 ggaggggctt atggcctttg aacaaacaaa tgggcgctat gcaagttttg gagttgtgac
     3061 ctcgcttcct ggggaagtga ttgatagttt ctggtatgtt attgatcact acttaaaagg
     3121 ggttattcca ttaaaaagtg taatccgttt ttctatcaaa aatcgtcgtg gtaagattac
     3181 gctggtcttt tcgcaggaag gttataaaaa tgtactggct gtagatctaa gtagtcgttt
     3241 tgatcctttt tatccctcaa caatcttggt aatggacaag cagggaaaag agactattac
     3301 tttgccagat gaggtgacct tgctttagct tgaagagctg gtattttatc acctaagata
     3361 ttgttaatat atgaaatttg gtgtaaaata gatttgtaag cttatgtggg ttagaaagga
     3421 ggagtctatg gttagtcaaa ctgttgaaaa acctctctat ttgcagttgg ttgatgaatt
     3481 ggaagttgca attcgtgaga ggatggctcc gaatgacaag ttattttcag agcgtgagtt
     3541 gacacaggtt tatggtgtca gtcgtattac agttcgtttg gctttgcaag aattggaaaa
     3601 acgtggctta gtctataaga agcatggtaa gggaacctat gtatctgaga tttcagatac
     3661 tgcagttgat ttgtctcagg cctatagttt tactgagcaa atgaaaaaaa ttgggaaagt
     3721 accgaggaca tctattttat cctttgaatt ggttaaggcg tcggactata ttgcgcagca
     3781 tctacaactt agtcagggtg aggaagtgtt tgaagttgag cgtttgagac ttgcagatga
     3841 ggttccgatg atgttggaaa ggacatatgt tcctgcctcg ctatttcctg gattagctgc
     3901 tcagcgaatg aagtcaactc ccttgtatga aattttttca gaggattatc atcaggtgat
     3961 tcgtttggca gaagaagagt tctatgcaag tattgctttg gataatgaag ctaagatact
     4021 cggaattcca agcaatagtc cagtgcttca tttggtaaga aaaacgtata atgataagaa
     4081 tagaattatc gagtttacgt ttagtattgc tagagcggac cagtttcgtt acaagattac
     4141 ccatcaacgg ggatattaga atcaatactc ttcgaaaatc aaaactattc gttgtcaaga
     4201 gccttgatga aatccagttc tatcttcgcc ttcgtttcct aggctacttt tgattgttat
     4261 tgggtatgaa aaaaacgcgg acgttagtct gcgttttttt agcgatggtt ggctgtaatt
     4321 tcaaatttgt aatacttcca gtgtttgtaa gtttcagtat attccatcac ttcaccgcta
     4381 gtactgttag tagttgtttt gttttgtagt acagctggtt cagtcgcttt taatttcaaa
     4441 tgggtggcaa cctcttttgg acatgggctg acaatttcat tcgtttctac aaatggttcc
     4501 tcggacatgt ggatattgta gtccagtttg aagcgttgat agattgattg atagtgctcg
     4561 agtggagcat cgggattctg gatatatcgt tgagggatgt atgaattgtg gaagatgtat
     4621 ggagtgtctt ctgctgcgcg gacacgcaca attttatagt aaaactcatt cttgtcaaga
     4681 tttagttttt caaggatatc gggtttattg cctttttcgc acgagagaac agtaacctta
     4741 tccttatcca taggaaaaat ctcaatatca gaaaattcta caagacgtcc tttgcgtgaa
     4801 cgagatacaa aggttccttt accttgttgg cgaacgaggt aaccatcttt aactagttcg
     4861 tttactgcac ggataacggt aatggagcta acattaaata gttttgttaa ttcagattct
     4921 gtgtaaaatt tgtcgccatt ttcaaatttg ccagaaacaa tctgttggcg taagtcgttt
     4981 tgtataagtt ggtacttcgg tactttcata cttatttcct tcctttccac agcttattat
     5041 actcaattag ttgatgaaaa tcaaaaaata tagctaaaat acccacaaaa atatactttt
     5101 tcactttata aaggtgataa gaaatggtaa aaaaattttt aaaaagtact ggacataacc
     5161 acaaataagt gttagtatat taatagaaag gtaagcggtt gcatcggagg aagaaatgaa
     5221 agaattttat attggagacc aattttattt ggacggtgaa ccctttaaga ttttatcagg
     5281 cgcgattcat tattttcgtg tccatcctga tgattggtac cattctttgt ataacttaaa
     5341 ggcccttggt tttaatacgg ttgaaaccta tgtcccttgg aatatgcatg aacctcgaaa
     5401 gggtgagttt tgttacgagg gtatattgga tattgagcga tttttgaaat tggctcaaga
     5461 attagggctt tatgcaattg tacgtccttc cccttatatt tgtgcggagt gggaatgggg
     5521 tggcctccct gcttggttga tgaaggaaga gcttagagta cgttcgagtg attcggtgta
     5581 cttgcagcac ttagatgaat attacgcttc tcttattcct aaattggcca agctccaact
     5641 cgcacagggt ggaaatgtac ttatgttcca agtggaaaat gagtatggtt cttatggtga
     5701 ggagaaggag tatctaagat cggtggctgg cttgatgcgt aaacatggtc taactgctcc
     5761 cttgtttacc tcagacggtt cttggcgagc aacattacgt gcaggaacac tgatagagga
     5821 cgatgtattt gtaacaggaa actttggttc taaagctaga gagaattttg caaatatgac
     5881 ggcattcttt aatgaacatc agaagaattg gccactcatg tgtatggaat tttgggatgg
     5941 ttggttcaat cgttggggcg atgaaattat tcgtagagag ccggaggaaa tggttgattc
     6001 ggttatggaa tgtatcgaat tgggctcctt gaatctgtac atgttccatg gtggaacaaa
     6061 ttttggtttc atgaatggtt gctctgctcg tggtcaaatt gatttgcctc aggtaacttc
     6121 ttatgattat gatgctatat tggatgaagc tggaaatcct actaagaagt tctatatttt
     6181 gcaacaacgt ttgaaagaag tctatcctga attagagtat gcagaaccgc ttgtaaaaga
     6241 ggcaaaagct ttttcagatg tgtcgcttca tgataaggtg agtttatttg ctactctaga
     6301 aaatgtaagc ggttgcgtaa aagggtttta tcctaagaat atggaagaac ttgatcagtc
     6361 aacaggttat attttatata gaacagagtt ggagcgggat aagacagaag cggaacgttt
     6421 ccgagtagtt gatgcgcgtg accgtatcca aatttatgca gatggaaaat tcgttgcaac
     6481 tcagtatcag acagagattg gtgacgatgt tgaattggat tttaaagatg acaagctaac
     6541 attagatatt ttggtagaaa atatgggacg tgtcaattat gggcacaaac taacggctcc
     6601 tacccagtct aaagggcttg gtcgaggtgc gatggctgat ttacatttta ttggtcattg
     6661 ggaaacctat cctttgcctc ttgattcagt tgaggacctg gattttagta agggctggga
     6721 ggaaggtcaa gctgctttct atcgttatca gtttgaactg gatgaacttg cggatactta
     6781 cttagatatg acaggttttg gtaagggagt tgttttcgtt aataatgtaa atattggacg
     6841 tttttgggaa aaaggaccaa tcctctatct ctatattcca aaaggatact taaagaaagg
     6901 agcgaatgaa atagttgtct tcgagacaga aggaaaatat agagagaaga ttcatttttc
     6961 acaaagaccc gttataaaag acttgtagaa attttaatcg gtttctgctc aaaagattta
     7021 tgaagaaaag gagaacactg tatggcaatt attgctacgc gtattgatgg tcgtttgatt
     7081 cacggtcagg ttgctaactt atggacgacg aaattgaaca tcggtcgtat tatggttatc
     7141 gatgatgctg ttgctcaaaa tgacattgaa aaacaaggac tgaaattagc atgtcctcca
     7201 ggcgtaaaat tatcgatttt accgattgaa aaggctgcta ataatattaa ggagggcaag
     7261 tatgatagtc aacgtctctt gattgttgct cgtcgtccag aaaacttcct tcgcttggta
     7321 gagtatgggg ttgagctccc tgagttgaac gtagggaata tgtctcaaac tccggaaact
     7381 cgttctgtaa ctcgttctat taacgttgta gataaggata ttgcagattt tgatgccttg
     7441 gcggctaagg gtgtaaaatt atttgcacaa atggtacctg gcgattcacc aaaagacttt
     7501 atgccattat tggataaggt tagataacat tcggatattt ttgaaaaatt taaaggagga
     7561 tcactattat gcaatggtgg caaatattac ttctaacgct ctactctgct taccagattt
     7621 gtgacgagct gacaattgta tcgtcagcag gttcaccagt ctttgcaggg tttatcactg
     7681 gtttaatcat ggggaacatg gaagttggct tgctaattgg tgcaagtttg cagctagttg
     7741 tcttgggagt tggtactttc ggtggtgcgt cacgtattga tgcaacatct ggtgcggttc
     7801 ttgcgacagc tttctcaatt tcacaaggtt tagatccaga aattgctatt tccactattg
     7861 gcgtaccagt tgcaggattg ttggtctata cagatatttt gggtcgtttc accactactt
     7921 tctttgctca tcgtgtagat gcagcaatcg aacgttttga ctatgctggt attgaacgca
     7981 actaccttct tggtgcaatt ccttgggcac tttctcgtgc acttccagtt tttcttgccc
     8041 ttgcttttgg tggtggtatt gtagaaactc ttgtcactac gattgagttg cctgaataca
     8101 aatggattgc agcaggctta actcttgcag ctcgtatgct tccaggtctt ggatttgcaa
     8161 tcttacttca ctatcttcca cttaaacgta accttcacta cttggcagta ggtttcgctc
     8221 ttacagctat gttgactgtt ctttacggta acgtatcagc tttgggtggt gcagttgctg
     8281 gtatcgttgg cactcttcct gaagatgcag gcgtaagctt tgtcaataac ttcaaaggtt
     8341 tgtcaatgat tggtatcgct attgtgggtg cattcctttc agtgattcac ttcaaaaata
     8401 gccaaaaagt aactgtggtt gctccatcaa attcagaaag tggggaaatt gaagatgacg
     8461 aaatctaatt acaaattaac aaaagaagat tttaatcaaa tcaacaaacg tagcttgttc
     8521 actttccaac ttggttggaa ctatgagcgg atgcaggcat ctggctacct ctacatgatt
     8581 ttgccacaat tgcgtaaaat gtatggagat ggtactcctg agttgaagga aatgatgaaa
     8641 ttgcatactc agttcttcaa tacttcacca ttcttccata ccatcatcac aggtattgac
     8701 ttggcacttg aagaaagtga tggcgttgct tctaaagatg cggttaacgg tgttaagaca
     8761 ggtttgatgg gaccatttgc tccaatcggt gactcaatct ttggttctct tgttccagcg
     8821 attatgggga ctgtggctgc tactatgggt aaagagggat ctccagttgg tatctttctt
     8881 tgggtagcag tagccattgc ctatgatatt ttccgttgga aacagttgga agttgcctac
     8941 aaagaaggta ctaagttgat tacaaccatg cgcgatcgct tgacagctct cgttgatgca
     9001 gcatctgtaa tgggtgtctt catgatggga gccctgattg caactatgat taactttgaa
     9061 gtgacttggg ctccagctat tggagaaaaa gtgattgata ttcaagacat tttgaatacc
     9121 atcttcccac gcctagtacc tgcaatcttt acaggatttg tcttctggct tcttggtcgt
     9181 cgtggtatga cttcgactaa agctatcttg attattattg tgcttgcact cggcttgtca
     9241 gcaatcggtc acttcctatt tggtatggta taatggttag aagtttagta ttagtaagtc
     9301 atggcatctt ctgcgaggag ttgaaaaaat ctactgaaat gattatgggt cctcaagagg
     9361 acatctacac agtagcttta ttaccagaag aaggtccaga agacttccag aaaaaatttg
     9421 aagaaacaat tgcaggattg gaagatttcg ttgtcttcgc agacttgttg ggcggtacac
     9481 cagccaacgt tgtatcacga aaattgattg aaggtgggca gtttgacctc tatgcaggga
     9541 tgaacatgcc aatggttatc ggtttcttga acggcgtcct gctgggagag gctgtggact
     9601 atgtagaatt tgggacaagc aacctagttc atgtcaatag tctattaaca agtgatgaag
     9661 atgacgatga gtgaaacagt ttggggaact gtttcaactg gtcaccttga aatgaaagag
     9721 tgaccaaaga cagcccgagc ctagaaataa gagagtgagg ctaactcagc cgattgttac
     9781 agacggattt gattttcgtt aattataaaa tgttctatag ttgataggct ttaagtatcc
     9841 tgagggagtt gcaatagtga ctccctcatt tggcttgtga ggaaaagaaa tgattgaagt
     9901 gaaaacattt ggtggacaag caaaactgta ttgcttggaa aacaagaatg ggatgcaggt
     9961 aaccttgacg gattttggtg ccagagtggt ggaagtgctt ttgccagtag aagaaaatgg
    10021 cggtctacgg aatgtcagtt tggctgccaa gtcggatgat gattaccgca agacagattt
    10081 gtaccctggt tcaagcattg tcccagtagc ggggcgtatt tcaggtgctc aggctgagat
    10141 aaagggaacc agttatcagt tcacagaaaa tgagccaggt cgtaccttgc atggtgggat
    10201 tgacacggca aatgaacagt actgggatgt tgctctagat catgagaaaa atcaagttac
    10261 ctttgggatt gtcttaaaag atggtttcaa tggttttcca ggagatgtga gagttgaggc
    10321 tatttatggt ctaacagacc agaatgagtt gacgatagat tatcgggcgg tgtctagcaa
    10381 agatacgatt ttcaatccga ccaatcacat ctatttcaac ttaacggggg attttcagca
    10441 gtcagtggca gagcacgaga ttcgaattgc ggctaatcgc tatgctccgc ttggtgagga
    10501 taatctgcca accggtgtct tagaggatgt gacaggaaca ccgtttgact ttagggagag
    10561 agcaagattt gctcagggct ttgacagcca gcaccctcag aatatccttg tgaaaggtta
    10621 cgaccatcca tggcttttgg aacaggtaga ggttccgatt gaagttgtca gcccagatgg
    10681 gaaaataagt ctgcgcgtgc gaaccaatca ggcatcagtt gtcatctaca cctacaatta
    10741 tcctgttgaa gctttggctg cctaccatgg tgcctttagc ttggaatgcc aggctctgcc
    10801 aaatgcttgc aacctaggcg gttttggttc tattttgctg gaaaaagacg aggaattctt
    10861 gtcccagact gtttatcaat ttacttggta agaaggagag ctctaactgt gttgcagtta
    10921 ggcttttctt gtgttttcta aaaaatatgg tataataaac cggtgtgttt caaacgaagc
    10981 agacgaaaga ggaaaataat gacaaaagaa caagaatttt taaaagaatt tgaggcttgg
    11041 gtcaacactc aggtcatggt aaacgagatg gcggttgaag aaagtcgtcg tgtcttggaa
    11101 gaagacaagg atgagcgtgc agcggatgct tatattcgtt atgagagcaa gctggatgcc
    11161 tatcgattca ttcaagggaa atttgccaac taccatgctg gcaaaggttt tcatgatttg
    11221 ccagatgagt tgtttggtca aaggcattat taaggagagg agttaacatg gcaaagaaaa
    11281 aagtaaatcg aaaaaaacag ttgaaaaaac aattagcaga cttgaaacgt gcaggtcgtg
    11341 taggtcttga aagagctgca gaggtagtag agacagttgc tcacaaggct gaggctgtag
    11401 tagagcatgc tgtggaacaa gtgaaagaag ttgtagcaga ggtgacttct tcagcgactt
    11461 cattggaaga ctttttggca cttcctgagt tggaaggcat tgcggcagca cgtttggaaa
    11521 ccttctacga agcgggtatt caatctgtgg ctgattttgc aaaccataca gaaaaagagc
    11581 tcttggctct taaaggcatc ggtcctgcaa ctatcaagca gttgaaagaa aaagggattg
    11641 agttgaaagc ttaaatccct tgcttttcaa ggtgcaattt gttacaataa agccattcag
    11701 aggtgttttg atctccattt gtcacaggaa gcggtggtac tgagaaatcc gcacaaatgt
    11761 caaaactggt tgctgatgat gtgaatcgaa taagcagagt gcagataatg tatctgaaca
    11821 agggtggtac cgcggaataa cttcgtccct gtctagcaag tctaggcggg gattttcatt
    11881 tggaaaggag aaaatatgtt acatcatgtt gaaatttacg tttctaattt ggaaacctcg
    11941 cgtgcatttt acgactttct cctgaccaag ctgggatact cgctctatca ggagtgggag
    12001 gaagggctat cttataaaaa agcagagcag tacttggtct ttgtccaaac gccacaggac
    12061 ttcttaaagg caggttatca ccgctgtcgc acaggtctca atcatctagc ttttcatgtg
    12121 ggaacacctg atgatgttga ccaatggcgg aaggaatttt tgaccagacg agtcaaattg
    12181 ctttacaatg accgctatcc ctacgcggga ggaccagacc actacgccct ctatttggaa
    12241 gacccggacg ggattaagat agagttggtg ggggaggtaa catgaattta gtaattattg
    12301 gagcacaagc ttctggcaag atgaccattg ggcaggaagt tgcgaaattg acagggatga
    12361 ccctttttca taatcacgat tcgattgatt ttagcctacg ttttattcct gaattttctg
    12421 aggatatgtt tgacctcaat acacgtatta catttgctgt ttatgatgtg tttgctaggt
    12481 caggccgccc actaattgga actgccttga ttaattttaa aaatctcata gaggttcaat
    12541 tccttacaac tgttcagacg atttttcatt atcatggtca ggaaatttta tttgttgagt
    12601 tggagactgc tttggaagaa cgtctgcggc gtaatcggac agagaatcga ctgacccata
    12661 aacctttgaa acgacacatt gaagtttcag aggctgagat tctctctaca gctgatacat
    12721 gtcaatatac ctcattaggc attccagagg gcattcagca ttatctcaaa attaataata
    12781 ctcaccttac tgctaatgaa gttgctcaac tgattatcaa aaaaatggag gaacttgaac
    12841 aaaaacaaac gaaatagcct ttccgttgag gggcgaaaca gaatataatc cagataggca
    12901 taaactattt ctattagtac tatcagttca gtcctgctac tatattacat catgcttatc
    12961 tcatggagca tactattttg cgtgcagtgt attaaaatat gaattaggag tatactgtct
    13021 atgaaaatgt taaacaagtt cacagagaat tcaaaagatt tgagaaatga gttgactagc
    13081 tggtttgtca tcatttcgct agccttctta ttattcttga gcattccttg gttggtgcaa
    13141 aagactttat attttacttt atcaaaacca tttgaagcac aaagagtagc catcacaaaa
    13201 tcaaacaaaa cttcagatac tgcctctgga gttgaaggga cctacatcaa taatattccc
    13261 tttaatgatt ttgaaaaatt gatgaatttt agcaatgtgt ctttcaaaa
//
LOCUS       DASFSW010000049        13272 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.049, whole genome shotgun sequence.
ACCESSION   DASFSW010000049 DASFSW010000000
VERSION     DASFSW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.049"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            120..1214
                     /locus_tag="U1099_001042"
     CDS             120..1214
                     /locus_tag="U1099_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM4867372.1"
                     /translation="MFHFTTLFIGMDVHKESFLLCYYDMMANQFKHSTKVGPNVSYIV
                     NYVNELRRLYGQDAEVLCGYEAGCLGFTLYHQLQAHGIPCIVMAPTTVMKEGSKRVKT
                     DKKDAAQLAKALAFRSYRPVHIPTAEDEQVKEYIRMRTDHKVALKKIKQQLLAFCLRH
                     DFRYTEGRNNWTQKHVRWLHSLNPKGLYAEILTEYLLTYEKLVDQIARYDARIEQLGQ
                     SDSYQEEVSQLSCFIGVKTLTALSIVTEIGDFNRFATAQHSASYLGLTPSENSSGDKE
                     RRGAITKAGNSHVRRLLIEAAQSLAKGTIGYKSKELKRRQSGNRVEVIAYADKANERL
                     RRRYRTLVLGKNKKQNVAKVAIARELSGFI"
     gene            2125..2940
                     /locus_tag="U1099_001043"
     CDS             2125..2940
                     /locus_tag="U1099_001043"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEM4867373.1"
                     /translation="MNQQFSLDQFSLKGKIALVTGASYGIGFAIASAYAKAGATIVFN
                     DINQELVDRGMAAYRAAGINAHGYVCDVTDEAGIQAMVAQIESEVGIIDILVNNAGII
                     RRVPMIEMTAAQFRQVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETV
                     SAYAAAKGGLKMLTKNIASEYGEANIQCNGIGPGYIATPQTAPLRELQKDGSRHPFDQ
                     FIIAKTPAARWGEAEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPEA"
     gene            2992..3633
                     /locus_tag="U1099_001044"
     CDS             2992..3633
                     /locus_tag="U1099_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEM4867374.1"
                     /translation="MKIALINENSQAAKNSLIFETLKSVTDAKGYHVFNYGMYGKEGE
                     SQLTYVQNGLLASILLVSKAADFVITGCGTGVGAMLAANSFPGVVCGFAVDPTDAYLF
                     SQINGGNALSLPFAKGFGWGAELNLKLLFERLFAEEMGGGYPRERVIPEQRNARILNE
                     MKAVTYKDLLTVLQEIDQDFLKETISGEHFQEYFFANCQDESIAAYLKTVLES"
     gene            3650..4651
                     /locus_tag="U1099_001045"
     CDS             3650..4651
                     /locus_tag="U1099_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEM4867375.1"
                     /translation="MKKVLLIGEPLIRITPTNYQELTDGVESRVFFGGSEINVACNLQ
                     GFGCETKLLTALPQGSLGDRFLDFLDGKGIDTSSVQRKGERVGVYYMEAGFGCRSSQV
                     YYDRGQSSLEAIQVEHFDMEQLFLEVGLVHFSGITVAIGSAVRAWLVQVLKEAKRRKI
                     PISMDLNWRSKMIGLRDAKKVFSQFATYADYCFGLEPIMANEKDWELFERDTATVHTI
                     RERMQTLKEAYKFQAIFHTIRQTDELGRNLYKAYGLADEFCCSQELKTQVLQRVGSGD
                     AFVAGALYKLLEGTSLKAVLDFAVASGTYKCSIEGDHMRQSLSKVESLLEGKNDIIR"
     gene            4662..5303
                     /locus_tag="U1099_001046"
     CDS             4662..5303
                     /locus_tag="U1099_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEM4867376.1"
                     /translation="MSPSQIISKLKEQGLVVVVRGDDKEVGFKTAQACIAGGITAIEV
                     AYTNPDANMIITELSRCHKDDVSVLIGAGTVLDSVTARQAIMAGASYIVSPSFNIEVA
                     KICHLYAVPYLPGCMTLTEITNALEAGCEMVKLFPGSVLGTNYITAIKGPLPHVSIMV
                     TGGVKLDNVSEWFNAGATVLGIGGEFNQLATKGEFGTIQELASSYASVRDKMK"
     gene            5589..6023
                     /locus_tag="U1099_001047"
     CDS             5589..6023
                     /locus_tag="U1099_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM4867377.1"
                     /translation="MIKVIVVAHGQFASGILTSLKLIAGEVENIEAIDFSEGMSAQEL
                     KARIKSAILGEREVLILTDLLGGTPFKVSVELAAEQKEQNVVVLSGLNLAMILEANFS
                     RLTDDLEQLVGKLITTSKDGIVDSGSLLAEDNHDEELFEDGI"
     gene            6035..7225
                     /locus_tag="U1099_001048"
     CDS             6035..7225
                     /locus_tag="U1099_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM4867378.1"
                     /translation="MIKDVLIESIAKKREFRNSTLLSKKEVEAAIELAIQQLVINCEY
                     FGESFPTPATFDNHYPKMDNTEWTNGFWTGELWLGHEYSLNNEMKALAKKNTTSFLER
                     VKQRIELDHHDLGFLYSPSTVADYKLTGSKEALEASLLSADKLMERYQEKGGFIQAWG
                     QLGKAEDYRLIIDCLLNLQLLFFAYEETGEQKYYDVALSHFYASVNTVIRDDASTFHT
                     YYFDPETGKPLKGVTRQGFSDDSSWARGQSWGIYGIPLAYRKIKDPACLNLFKGVTNY
                     FLNRLPEDNVSYWDLIFNDGSGHARDSSATAIAVCGIHEMLKYLPEVDEDKETYKYAM
                     HTMLRSLIDNYANRELAPGRPLLLHGVYSWHSGKGVNEGNTWGDYYYLEALLRFYKDW
                     ELYW"
     gene            7235..7726
                     /locus_tag="U1099_001049"
     CDS             7235..7726
                     /locus_tag="U1099_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEM4867379.1"
                     /translation="MATPNIVMARIDERLIHGQGQLWVKSLGCNTVIVANDKVSEDKI
                     QQTLMKTVIPDSVAMRFFSLQKVIDVIHKANPAQTIFLVIKDLQDALTLVKGGVPITE
                     LNIGNIHNAPGKTQVTRSIFFGSEDKAIVRELNDVYKIRFNTKTTPSGNDGAVEVNIL
                     DYI"
     gene            7746..8522
                     /locus_tag="U1099_001050"
     CDS             7746..8522
                     /locus_tag="U1099_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEM4867380.1"
                     /translation="MTISLIQAALIGLWTAFCFSGLLLGLYTNRCIVLSFGVGIILGD
                     LPTALAMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVGIQFLQTSAYTLHAGAPESAIKHLRAGNLSKFKRVANGTVITFAFLGFALGFLG
                     AYSMDTLLKLVEMIPPVLLTGLSVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLQLPVIGIAIVGSIFALKEFFNKPEQVAVKQEEAHDDWI"
     gene            8509..9324
                     /locus_tag="U1099_001051"
     CDS             8509..9324
                     /locus_tag="U1099_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEM4867381.1"
                     /translation="MTGSKLTKKDYLNTSLRAFFLQNGFNYSNYQGIGYANVMYPAFK
                     KHFGDDKEGLTKALEENCEFYNTNPHFLPFITSLHLVMLENDLPEEETRGIKMALMGP
                     LAGIGDSLSQFVLAPLLSTIAASLAGQGLVMGPILFFLGMNIILTSIKLLTGLYGYKF
                     GTSVIDKLSDQMATISKVANIIGVTVISGLAVTFVKISVPITFAAGELTEGADQKIVT
                     IQGMLDKIAPALLPALFTILVYYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            9331..9639
                     /locus_tag="U1099_001052"
     CDS             9331..9639
                     /locus_tag="U1099_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEM4867382.1"
                     /translation="MEQQTLYYVLIILVALSTILPVLTSYINRKRQQKALEEQLEKRQ
                     RYLSTLQAGDEVILFSGLHGKIIKLTDSLVELQIAEGVLVYVEKESIMGKAKELLFTK
                     "
     gene            9736..13230
                     /locus_tag="U1099_001053"
     CDS             9736..13230
                     /locus_tag="U1099_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEM4867383.1"
                     /translation="MGFFISQSKQHYGIRKYKVGVCSALIALSILGTRVAANQLPSAE
                     TASPQSSQLVETTPETTEAVMTSEVSSEVSPVTSTETQPSSTTAETLASPQAVQATKE
                     EKNLVANGEFISTTAPSGNWKELAATNWETWIPANVKKENGQVRIDEGRLHISSTASY
                     RVAVHQTVDVDPNKRYLFSYDVETKDLKGSGVRVRLRSLTAEGKDLSPQEFAYTPYKN
                     GSQAEHIEQILTVSPETRKLKVELFFENSVGQAWLDNISLVEYVEKTPETPEQSPELV
                     QPETGQISLASNKVYLPVRPDLTYRIADAAVATVEKNMIRPLAAGKTQVDVYDKDTKL
                     SSFELIVTEHQATVFDTLRNNWEDISLANKRYQSNDAQMKAFLGRLDAGVASSLEKWV
                     EPTEQSKTIFNDIDFSKSSHLTTVYRRLEQMAQVVENPDSAYYHDRSLIDLVRKGMNW
                     LYTNVYNENKSIDGNWWDYEIGTPRAVVNTLIYMHPYFSQEEILTYTKPISKFVPDPT
                     TIRKTLTNPVPAVGGNQTDLSKVAILEGALREDADRVRAGAQGLTTIMKFVDKGEGFY
                     RDGSFIDHTNVAYTGAYGNVLIEGFSQLLPVIQPTEFALKEEQTNILYEWIEKAFMPI
                     LVRGELMDMTRGRSISRATGESHVQAMEILRSLVRIAESAQPEQKTKLLSFVKAQLTS
                     DTFYDSYRSLKSYKDIDLVNKLLADNQIPAEVDKDYIAAFNNMDKFVYRSAQEGFTFA
                     LSMYSSRTQNYEDMNNENRKGWYTADGMVYLYNDDLSHYSNHYWATVDPYRLPGTTTT
                     KDKREDGSGEVTLASDFVGASQLGNRLATIAMDFNNWNNSLTARKAWIVLGNKIVFLG
                     TDIQHQSAQGAETTIENRKLLTGEKYSYYINGQPVDLSKEVVTDKTQSFYMTNGKDNQ
                     SIGYVFLNQLPTHAKLDQRTGKWSDINYNQSKEEVSNSFVSLWHEHAQTSSNYAYVLV
                     PNQSMEKVNQAAASVKLLHQDRDLQVVYDQEQNVWGVVKYTDTAYKLTDDITLTDAGL
                     YTIQKVEGGYRIAFYNPSTRTVKNGIELTKAGSSLTVEMEPTAAYPSTVWKVTMPEGS
                     DKQTGSVEKTEKEEKQLKENQPSSEVKQVVHHAAEKTKPSKPRLPQTGEEASLGLGFL
                     GLLTLGAVVDFKCRRSHS"
BASE COUNT         4062 a         2318 c         3078 g         3814 t
ORIGIN      
        1 tttttggtat agacaatatg taaagacccc cagttgagaa ttcgtggatt tacctgtaca
       61 ctagaataaa aaacaatcaa cttctaacag gaattaccac actcaattgg aggtcttata
      121 tgtttcattt taccacactt ttcatcggaa tggatgttca caaagaaagt tttttactct
      181 gctattatga tatgatggcg aatcaattca aacatagcac taaagttggt ccaaatgtta
      241 gctatattgt gaactatgtg aatgagcttc gtcgtttata tggtcaagat gcagaagtgt
      301 tatgtggcta cgaagccgga tgtcttggat ttaccctata tcaccagcta caagctcacg
      361 ggattccctg tatcgtgatg gcgcctacaa cggtgatgaa ggaaggatct aagcgtgtca
      421 agactgataa gaaagatgca gctcagctcg caaaagctct ggcctttcgt agctatcggc
      481 ctgttcatat tcctactgct gaggatgaac aagtcaaaga atatatccgc atgagaacag
      541 accacaaagt ggctctgaag aaaatcaaac aacaacttct tgccttctgt ctccgacatg
      601 attttcgcta taccgaggga agaaataatt ggacacagaa acatgtccgc tggctccatt
      661 ccctaaatcc taagggactt tacgcagaga ttttgacaga atacctattg acctatgaga
      721 aattagtaga tcaaatagca cggtatgatg cgcgaattga gcaactgggt caaagcgaca
      781 gttatcaaga ggaggtctct cagctttctt gctttattgg cgtcaaaaca ctaaccgctc
      841 tttccattgt gacagaaatc ggtgatttta accgctttgc gacagctcaa cattctgctt
      901 cttatcttgg gctgactcct agcgaaaact ctagtggcga caaggagaga agaggtgcta
      961 tcaccaaagc tgggaatagc catgtgagac gacttctgat agaagctgca caatcattgg
     1021 ccaagggaac gattgggtat aaatccaaag aattgaaaag gagacaaagt ggaaaccgag
     1081 tggaggtgat tgcttatgcg gataaggcta atgaacgctt aagaagacgt tatcgcacac
     1141 ttgttctagg aaaaaataag aaacaaaatg ttgctaaagt agctattgca cgggaattat
     1201 ctggttttat ttaggggatg atgacaggaa gaatagcttg aagttagcgc ttattttcat
     1261 gtacactttt gacaattaag ttttatcatc gcagagcgat gaggaatccc tatttctatc
     1321 ccaaatcact gggtgaattt ggacagaaat aaggattgaa ataatgtttg aatggaaagt
     1381 atcttgaagg agtttaggac ttcaaggttt gagtcatcta cgaaacgact aagtggcaca
     1441 attgtaatcc acgcttatag agagtcggac tcgcgtcgcg gcagaaccat tgacctgtag
     1501 gtaaccaaag ctacaggttg ctagaatagc ctaaaagctg ttctaggttg gaaataagga
     1561 aacgacgttt cctcgctcgt cgaagtaatc aaagataaac tgatatgact atataaaatg
     1621 aattacagaa tatatgattg agatagatgg taccttgttg cacgttgcaa tagggtattt
     1681 tttatttaga gtagtaaaca cagggtaaaa atgttaatgt ttgagttcct attaacatct
     1741 cggcggagtg gttgagtttt cgaatattct ggagattgtt tgaggtggca aattggtagt
     1801 gagggagtca gtcgtatcac ttcccgctcc cattttgtat ggtttacttt cgtgaatgta
     1861 gtatagccaa ttttggataa tatatgaata atgacatgta aaatgtttac taatgattga
     1921 taaaatcgaa gaagaaaatc atatttgtgt taagtatatt ttattgtttg gggaatgaag
     1981 atactcgaat tttataagat tatatcaaga ttcgttttga aagtatttaa aataaatgat
     2041 aaaaaccgtt gacaaaaccg gtttactata ttacaataca atttgtaaac gctttacaaa
     2101 tcaaaataca aaggagttac gaatatgaat cagcaatttt cattggatca attttctcta
     2161 aagggaaaaa ttgctttggt aacaggggcg tcgtatggga ttggctttgc gattgcatcg
     2221 gcctatgcaa aagcaggggc gacaattgtt tttaatgata ttaatcaaga gttggttgat
     2281 aggggaatgg cagcctatag ggcagctggt attaatgcgc atggctatgt ttgcgatgta
     2341 actgatgaag ctggaataca ggctatggtt gctcaaattg aatcagaggt tggaattatt
     2401 gatattctag tgaataatgc tggtattatt cgtagagttc caatgattga aatgacagcg
     2461 gctcaatttc gccaagttat tgatattgac ctaaatgctc cgtttattgt ttctaaagcg
     2521 gttattccat ctatgattaa aaaggggcat gggaagatta tcaatatttg ttccatgatg
     2581 agtgagttag gacgagaaac agtgtctgct tatgcagcgg ctaagggtgg gcttaagatg
     2641 ttgacgaaga atattgcgtc ggaatacggt gaagccaata tacaatgtaa cggcattggt
     2701 cctgggtata ttgcaactcc acaaacagct ccgttacgtg aactccagaa ggatggttct
     2761 cgtcatccat ttgatcaatt tattatcgcg aagacccctg cagctcgttg gggtgaagct
     2821 gaggatttga tgggtccagc agtcttttta gcaagtgatg ctagtaattt tgtgaatggg
     2881 cacattcttt atgttgatgg gggaattcta gcttacattg gtaaacagcc ggaagcgtaa
     2941 taaacgaatt aacaaattta acaaattttg atataagaag ggaaaaaaac gatgaaaatt
     3001 gcacttatta atgaaaatag tcaggctgct aaaaatagtc ttatttttga aacattgaag
     3061 tcagtaacag atgctaaagg gtatcatgtt tttaactatg gaatgtatgg taaagaggga
     3121 gaaagtcagt tgacttatgt acaaaatggt ctgttggctt caatccttct agttagtaag
     3181 gcagcggatt ttgttattac aggttgtggc acgggggtag gtgccatgtt ggcggctaat
     3241 agttttccag gggtagtttg tggcttcgcg gtagatccga cggatgcata cctcttttca
     3301 cagataaatg gaggaaatgc tttgtctctg ccatttgcaa agggattcgg ttggggagct
     3361 gaattaaatc tcaaattact ttttgagcga ctgtttgcag aggaaatggg gggcggatat
     3421 ccccgtgaac gcgttattcc ggaacaacga aatgctcgta ttttgaatga aatgaaagca
     3481 gtcacctata aagacttact gacagtactt caggaaattg atcaagattt tttgaaagaa
     3541 actatttcag gtgaacattt ccaagaatat ttctttgcca actgtcagga cgagtcaatt
     3601 gcagcatatt taaaaacagt attagaaagt taaagagtgg ggggcggtta tgaagaaggt
     3661 attactgatt ggtgaacctt taattcgcat tacaccaaca aattatcaag aattgacaga
     3721 tggtgtagaa agtcgtgtgt tttttggagg ttcagaaatc aatgtcgctt gtaatcttca
     3781 aggatttgga tgtgaaacca agcttttgac cgcactacca caaggctctc taggtgatag
     3841 atttttagat ttcttagatg ggaaagggat tgatacttcc agtgttcaac gtaaggggga
     3901 acgtgtgggg gtatactaca tggaagcagg atttggttgc cgttctagtc aagtctacta
     3961 tgatcgtggc cagtctagtt tggaagctat tcaagtcgaa cattttgata tggaacaatt
     4021 atttttagag gttgggttgg ttcactttag tggtatcacc gtggctattg gttcagctgt
     4081 tagggcatgg cttgttcaag tattaaaaga agcaaagcgt cgtaaaattc cgatttcgat
     4141 ggatcttaat tggcgttcta agatgattgg attaagagat gcaaaaaaag tgttctcgca
     4201 atttgcgacc tatgcagact attgttttgg tctggaacct attatggcta atgaaaaaga
     4261 ttgggaatta tttgaaagag atacggctac tgtccataca ataagagaac ggatgcaaac
     4321 tttaaaggaa gcctataagt tccaagcaat tttccatacg attaggcaga cagatgaact
     4381 aggcagaaat ttgtataagg cttacggatt ggctgatgaa ttttgttgct ctcaagaatt
     4441 gaaaactcag gtgttacaac gtgttggcag tggtgatgcg tttgttgctg gggcattata
     4501 taaattgtta gaggggacat cgttaaaggc tgttttagat tttgcagttg ctagcgggac
     4561 ttataaatgc agcatagaag gggatcacat gcggcaatct cttagcaaag ttgagtcttt
     4621 attagagggg aaaaatgaca ttatacgata aaggagaaaa aatgagtcca tcacaaatta
     4681 tttcaaaatt gaaggagcaa gggcttgttg tagtcgttag aggagatgac aaagaagtcg
     4741 gatttaagac ggcacaggcc tgtattgcag gaggaattac agctatagag gttgcttata
     4801 caaatcctga cgcgaatatg attattacag aacttagtcg atgccataaa gatgatgttt
     4861 ctgttttaat tggggctgga acggttctag attctgtaac ggcacggcaa gccattatgg
     4921 caggagcaag ttatattgtt tcaccatctt ttaatataga agttgcaaaa atctgtcatc
     4981 tttatgcagt gccatatctt ccgggctgta tgactttaac ggagattaca aatgctttgg
     5041 aagcaggctg tgaaatggtt aaactgtttc ctggtagtgt tttggggaca aactatatta
     5101 ctgcaatcaa aggaccatta ccgcatgttt caatcatggt aaccggtgga gtaaagcttg
     5161 ataatgtttc agaatggttt aacgctggtg caactgttct tggtattggt ggggaattta
     5221 atcaattagc tactaaagga gagttcggta caattcaaga attagcgtcc tcttatgcaa
     5281 gtgttagaga taagatgaag tagtttattt caaaataagg actgtatttt taacgttttc
     5341 actttctcct gcctccaacg gggttgaatg tgggagaaag tgagatgtta caggggaaat
     5401 tttgaacgga aacatggaga tatttggtac tcctgtttat gaaaagaggg agctggagaa
     5461 tattttttaa ataaaagtcc aacaaaatat aaaacgctta cattttttat aaaatctatt
     5521 gacaagtggt tgtcaatagt ctacaataca aagtgtaggt aaaccggtaa agttaaaagg
     5581 agagaattat gattaaagtg attgtcgttg ctcatggaca gtttgctagt gggattctta
     5641 cttcactaaa gttaattgca ggagaggtgg agaatattga ggcaattgat tttagtgaag
     5701 gaatgtcggc gcaagaacta aaagcacgca tcaaatcagc tatattaggc gaaagagaag
     5761 ttttgatttt aactgattta ctaggaggta cgccttttaa agttagtgta gaattagcag
     5821 cagaacagaa agagcagaat gttgtcgttc tttcaggttt aaatcttgcg atgattttgg
     5881 aagcgaattt ttcacggtta acagatgatt tagaacaatt ggttggtaaa ttgattacca
     5941 cctctaaaga tggaatcgtt gattcgggca gtctgttagc agaggataac catgatgaag
     6001 agttatttga agatggtata tgagggagaa atgcatgatt aaggatgttt tgattgagtc
     6061 aattgctaaa aaaagagagt ttagaaattc cactctattg agtaagaaag aagttgaggc
     6121 tgcaatagaa ttggcgattc aacaacttgt tatcaattgt gaatattttg gtgagtcgtt
     6181 cccgactccg gccacatttg ataaccatta tccaaagatg gataatacag agtggacaaa
     6241 tggtttttgg acgggcgagc tttggttagg acatgagtat tctttgaaca atgaaatgaa
     6301 agcgttagca aaaaagaata cgacatcttt cttggaacgt gtcaaacaaa gaattgaatt
     6361 agatcatcat gatttgggat ttctgtattc accatcaacg gtagctgatt ataaattgac
     6421 aggtagtaaa gaggctttag aggcttcgct cttgtctgct gataaattaa tggagcgcta
     6481 tcaagaaaaa ggaggtttta ttcaggcctg ggggcaactt ggtaaagcag aagattatcg
     6541 gttgattatt gattgtttgc tgaatttaca attacttttc tttgcttatg aggagacagg
     6601 tgagcagaaa tattatgatg tagctcttag tcatttctac gcttctgtta atacagttat
     6661 tcgagatgat gcttcaacat ttcataccta ttactttgac cctgaaacag gaaaaccgct
     6721 caaaggggtt acccgacaag gatttagtga tgactcttcc tgggcgcgtg gtcaatcttg
     6781 gggtatttac ggtattccgt tagcttaccg taaaattaaa gatcccgcat gtttaaattt
     6841 gtttaaagga gtaaccaact actttcttaa tcgtttgcca gaggataacg tttcctattg
     6901 ggatttaatt tttaatgatg gtagtgggca cgctcgtgat tcttctgcga cagcaatagc
     6961 ggtttgtggt attcacgaga tgcttaaata tttgccagaa gttgatgagg acaaggaaac
     7021 ttataaatat gctatgcaca cgatgctacg gtccttgatt gataactatg ctaatcgtga
     7081 gttagctcca ggtcgtccat tgcttttaca tggggtctac tcttggcatt caggaaaagg
     7141 tgtcaatgaa ggaaatactt ggggcgatta ttattactta gaagctttgc ttcgttttta
     7201 taaagattgg gaattatatt ggtaggaggt taaaatggca acaccaaata ttgtaatggc
     7261 tcgtattgat gagcgactaa ttcatggaca gggacaacta tgggttaaat ccttaggttg
     7321 taatactgtc atcgtggcaa atgataaggt tagtgaagat aagattcagc agacattaat
     7381 gaaaacggtt attccagatt cagtagctat gcgtttcttc tctttgcaga aagtgattga
     7441 tgtgattcat aaggctaatc ctgctcaaac tattttctta gttattaaag atttgcaaga
     7501 tgcactaact ttggttaaag ggggagtacc aatcacagaa ttaaatattg gaaatattca
     7561 taatgcgcct ggaaaaacgc aagtaacacg gtctatcttt tttggttcag aagataaggc
     7621 tatagtgaga gagttgaacg atgtttataa gattcgcttc aacacaaaga caacgccttc
     7681 tggtaatgat ggggcagttg aagttaatat attggattat atttaaaaaa ataaggagaa
     7741 ttatcatgac gatcagtcta attcaagcag ctcttattgg tctgtggaca gctttctgtt
     7801 ttagcggtct attattgggg ctttacacaa accgatgtat cgtgctttct tttggggtcg
     7861 gaatcatttt aggagatcta ccaacggctt tagctatggg cgctatcagt gagctggcat
     7921 acatgggatt tggtgttggt gctgggggga ccgttcctcc aaatccaatc ggacctggta
     7981 tttttggtac tttgatggct attacaagtg ctggaaaagt aacaccagaa gcagctttag
     8041 cactttcaac acctattgcg gtaggtattc aattcttaca aacttcagca tatactttac
     8101 atgcgggtgc tccagaatca gctatcaaac atcttcgtgc aggaaacctt tctaaattta
     8161 aacgtgttgc aaatggtaca gtgattactt tcgccttttt aggatttgct cttggtttct
     8221 tgggtgccta ttcaatggat actcttttga aattggttga aatgattcca ccagtattat
     8281 taaccggatt gagtgtagca ggtaagatgc ttccagctat cggttttgca atgattttgt
     8341 ctgtaatggc taagaaagaa ttgatcccgt ttgtattgct tggctatgtt tgtgcagctt
     8401 atctacaact gcctgtaatt ggtatcgcaa ttgtcggtag tatctttgca cttaaagaat
     8461 tctttaataa accagaacaa gtggcagtga aacaggagga agcacacgat gactggatct
     8521 aaactaacta aaaaagatta tctcaacact tctttacggg ccttcttctt gcagaatggt
     8581 ttcaattaca gtaattatca agggattggc tatgcaaatg tcatgtatcc agcttttaag
     8641 aagcattttg gtgacgacaa agaaggatta actaaagctt tagaagaaaa ctgtgagttt
     8701 tacaatacca atccacattt tcttccattc attactagtc ttcatttggt catgttagaa
     8761 aatgatctcc cagaagaaga gactcgtggg attaagatgg ctctgatggg gccgttggca
     8821 ggaattggtg actcactttc acaatttgta ttagccccac ttttatctac gattgctgcc
     8881 tctctagcag gtcaaggact ggttatggga ccgattcttt tcttccttgg aatgaacatt
     8941 atcttgacct ctatcaagtt gttgactggt ttgtatggct ataaatttgg taccagcgtg
     9001 attgataagt tgagtgacca aatggcaaca atttctaagg tagccaatat catcggtgtg
     9061 acagttatct ctggtttagc agtaaccttt gtaaaaattt cggtgccaat cacctttgcg
     9121 gcgggtgaat taacagaagg agcagatcag aaaattgtaa ccatccaggg aatgttagac
     9181 aagattgccc ctgcgttgct tccagccttg tttacgattt tggtttatta tctcatcaag
     9241 aacaagaaat ggacaaccta caaactagtt atcctgacag ttatcatcgg tattttgggt
     9301 agctggctgg gcatccttgc ataagcgcct atggaacaac aaactctcta ctatgtacta
     9361 attattttag tagctctatc gaccatactt cctgtactca cttcttatat caatcgtaag
     9421 cgacaacaaa aggcacttga ggagcaactg gaaaaacgcc aacgctactt atcaacctta
     9481 caggctggag atgaggttat cctcttctct gggctgcatg ggaaaatcat caaactaaca
     9541 gacagcttgg tagaattgca gattgcagaa ggtgttcttg tttacgttga aaaagagtcg
     9601 attatgggaa aagccaaaga actacttttt acgaagtagt tcttctttct tgcctccgca
     9661 aggaagcggt tgcaggatta aaattatacc cctgggacat tgttttgccc cgcataaaaa
     9721 ggaaaggaaa caccaatggg attttttatc agtcaaagca agcagcatta cggcatccgg
     9781 aaatataagg tgggtgtctg ctccgccctt atcgcgctgt ctatcttggg aacaagggtt
     9841 gcagccaatc aactgccaag tgcagaaaca gctagtccac aaagcagtca gttagtggaa
     9901 acaactccag aaacgacaga agcagtaatg acaagcgagg tcagctctga ggttagtcct
     9961 gttacatcaa cagaaaccca gccttcaagc accactgcag aaactcttgc cagtccgcaa
    10021 gctgttcagg cgacaaaaga agagaagaac ctagtagcca atggggagtt catcagtaca
    10081 acggcaccat caggaaactg gaaagaattg gcagctacaa actgggaaac atggattcct
    10141 gcaaatgtga agaaagaaaa cggacaggtt cgcattgatg agggacggct gcatatctct
    10201 tcaacagcta gctatcgagt ggcagtgcac caaacagtgg atgtagaccc taacaagcgc
    10261 tatctcttta gctatgatgt agaaaccaag gacctgaagg ggagcggtgt tcgtgttcgc
    10321 ttgcgcagtt tgactgcaga aggcaaggac ttgagtccac aagaatttgc ctatacacct
    10381 tataaaaatg gaagtcaggc ggagcacata gagcagatct tgacggtatc tccggaaact
    10441 cgtaaattga aggtggaact gttttttgag aatagtgtcg gccaagcctg gctggataac
    10501 atctcgctgg tcgaatacgt agaaaagaca ccagaaacac cagagcaaag cccagaactt
    10561 gtccaacctg aaacagggca aataagtctt gccagcaaca aggtctacct gcctgttcgg
    10621 ccagacctga cctatcggat tgcggatgcg gctgtggcca cagtagagaa aaatatgatt
    10681 cgtccgcttg cggctggaaa gactcaggta gatgtctatg ataaggacac caagctatct
    10741 agtttcgagc tgatagttac agagcatcag gcaacagtct ttgatacctt aaggaacaac
    10801 tgggaagata tttctttagc caataaacgc taccaatcta acgatgctca gatgaaagcc
    10861 tttctagggc gtttagatgc cggtgtagct tcttctcttg aaaaatgggt tgaaccaaca
    10921 gagcagagca aaacgatttt caacgatatt gactttagta aatccagcca tctgacaaca
    10981 gtctatcgcc gcttggagca gatggctcaa gtagttgaga atccagactc agcctattac
    11041 cacgaccgct cacttatcga tttggtcaga aaaggaatga actggcttta caccaatgtt
    11101 tataatgaaa ataagtcaat tgatgggaat tggtgggatt atgaaattgg aactccgcgt
    11161 gcggttgtca acaccttgat ctacatgcat ccctacttta gtcaggagga gattctaacc
    11221 tataccaaac cgatttcaaa atttgtacca gatccaacca ctatccgtaa aacattgacc
    11281 aatccagtcc cagcagttgg cggtaatcaa acggatttga gcaaggtggc catcttggaa
    11341 ggagctcttc gcgaagatgc tgaccgtgtg agggctggtg cgcaaggttt gacaacgatt
    11401 atgaaatttg tagacaaggg agagggattc tacagggatg gctcctttat cgaccacacc
    11461 aatgttgcct acacaggggc gtacggaaat gtcctcattg aaggtttttc acagttacta
    11521 ccagttattc agccaacgga gtttgccctc aaggaagaac agaccaacat tctttatgaa
    11581 tggattgaaa aagcctttat gccaatattg gttcgcggtg aactgatgga tatgacccgc
    11641 ggtcgttcca tcagtcgggc aactggtgaa tcacatgttc aggccatgga gattttaagg
    11701 tctttggtcc gcattgcaga atcagctcag cctgagcaaa aaaccaaact tctctccttt
    11761 gttaaggcac agctgacctc tgataccttc tatgacagct accgcagcct caaatcctat
    11821 aaggatattg atttggtcaa caagctttta gctgataatc aaatccctgc tgaagtggat
    11881 aaagattata ttgcagcctt caacaatatg gataagtttg tttatcgaag tgctcaagaa
    11941 ggctttacct ttgccttgtc tatgtactct tctagaactc aaaactatga agatatgaac
    12001 aatgaaaacc gcaagggttg gtatacagca gatggcatgg tttacctcta caatgatgat
    12061 ttgagtcatt acagtaacca ctactgggca acagttgacc cgtatcgctt accgggaacg
    12121 acaacgacca aggacaaacg agaagatggc agtggcgagg taactcttgc aagtgacttt
    12181 gttggagcaa gccagttagg caaccgctta gcgacgattg caatggattt caacaactgg
    12241 aacaatagtc tgactgctcg taaggcttgg attgttctgg gaaataagat tgttttccta
    12301 ggtactgata tacagcatca atctgctcaa ggcgccgaga caacgattga aaaccgtaaa
    12361 ctcttgaccg gagaaaaata tagctattac ataaatggcc agccagttga tttgtccaaa
    12421 gaagtagtga cagataagac tcaatccttt tacatgacca atggcaagga caaccagtcc
    12481 atcggttacg tcttcttgaa ccaactgccg acccatgcca aattagacca gcgaactggc
    12541 aaatggagtg acatcaacta caaccagtcg aaagaagaag tgagcaattc ctttgttagc
    12601 ctatggcatg aacatgcaca gacttcaagt aactatgctt acgtccttgt tccaaaccaa
    12661 tctatggaaa aagtgaatca ggcagctgct agtgtgaaat tgcttcatca agacagggat
    12721 ttgcaagtgg tatacgacca agaacagaat gtctggggcg ttgtgaaata cacggataca
    12781 gcctacaagc tgacagatga tattacctta acagatgccg gtttgtatac cattcagaaa
    12841 gtcgaaggtg gctatcgcat cgccttttac aatccgtcta cccgaaccgt aaagaatggc
    12901 attgagttga caaaggcagg ttctagtctg actgttgaga tggagccgac tgcggcttat
    12961 ccatccactg tctggaaggt tactatgcca gaaggttctg ataaacaaac tggaagtgtc
    13021 gaaaaaacag aaaaagaaga aaaacaactc aaggaaaacc aaccaagttc agaggtgaaa
    13081 caagtggttc atcatgctgc cgaaaaaaca aagccgagta agccccgtct gccccagaca
    13141 ggagaagaag ccagtctggg tcttggtttc ttaggtttgc taaccttggg ggcagtggtg
    13201 gatttcaagt gtcgcagatc gcactcctaa atgaaattcg tgggtgaagg gggagcggga
    13261 aagaactcga ct
//
LOCUS       DASFSW010000050        13111 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.050, whole genome shotgun sequence.
ACCESSION   DASFSW010000050 DASFSW010000000
VERSION     DASFSW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.050"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            80..962
                     /locus_tag="U1099_001054"
                     /pseudo
     CDS             80..962
                     /locus_tag="U1099_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            972..1175
                     /locus_tag="U1099_001055"
     CDS             972..1175
                     /locus_tag="U1099_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEM4867384.1"
                     /translation="MYQLGSFVEMKKPHACVIKSTGKKANKWEVIRLGADIKIRCTNC
                     DHVVMMSRHDFEQKMKQVLPSEA"
     gene            1279..1644
                     /locus_tag="U1099_001056"
     CDS             1279..1644
                     /locus_tag="U1099_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867385.1"
                     /translation="MNQLAQQIRILRTAKNLSQDELAEKLYISRQAVSKWENGEATPD
                     IDKLVQLAEIFGVSLDYLVLGKEPEKEIVVEQRGKMNGWEFLSEESKRPVENKEIFLF
                     ILSMAICIFVVWILTMITK"
     gene            1756..2871
                     /gene="ychF"
                     /locus_tag="U1099_001057"
     CDS             1756..2871
                     /gene="ychF"
                     /locus_tag="U1099_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEM4867386.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KDSVAEFAVLEKIKPVLEDGKSARTVDFTDEEQKIVKQLFLLTTKPVLYVANVDEDKV
                     ADPESISYIQKIRDFAATENAEVVVISARAEEEISELDDEDKGEFLEALGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQCAGIIHSDFEKGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            3029..3598
                     /locus_tag="U1099_001058"
     CDS             3029..3598
                     /locus_tag="U1099_001058"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEM4867387.1"
                     /translation="MTRLIIGLGNPGDRYFETKHNVGFMLLDKIAKRENVTFNHDKIF
                     QADIGTTFIDGEKIYLVKPTTFMNESGKAVHALMAYYGLDATDILVAYDDLDMAVGKI
                     RFRQKGSAGGHNGIKSIVKHIGTQEFDRIKIGIGRPKGKMSVVNHVLSGFDTEDRIEI
                     DLALDKLDKAVNVYLEEDDFDTVMRKFNG"
     gene            3598..7092
                     /gene="mfd"
                     /locus_tag="U1099_001059"
     CDS             3598..7092
                     /gene="mfd"
                     /locus_tag="U1099_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEM4867388.1"
                     /translation="MNILDLLHKNKQINQWQSGLNKSTRQLLLGLTGTSKSLVMATAY
                     DSMAEKIMIVTATQNDAEKLVADLVAIIGSENVYNFFTDDSPIAEFVFASKERTQSRI
                     DSLNFLTDSTSSGILVASMAACRVLLPSPETYKGSKIKLEVGQEIEVDKLVNNLVNIG
                     YKKVSRVLTQGEFSQRGDILDIFDMQAETPYRIEFFGDEIDGIRIFDVDSQKSLENLD
                     EISISPASDIILSSEDYSRASQYIQTAIEQSTLEEQQSYLREVLADMQTEYRHPDLRK
                     FLSCIYEQSWTLLDYLPKSSPLFLDDFHKISDKQAQFEKEIADLLTDDLQKGKTVSSL
                     KYFASTYAELRKYKPATFFSSFQKGLGNVKFDALYQFTQHPMQEFFHQIPLLKDELTR
                     YAKSNNTVVIQASSDVSLQTLQKTLQEYDIHLPVHAADKLVEGQQQVTIGQLASGFHL
                     MDEKLVFITEKEIFNKKMKRKTRRTNISNAERIKDYSELAVGDYVVHHVHGIGQYLGI
                     ETIEISGIHRDYLTVQYQNFDRISIPVEQIDLLSKYLASDGKAPKVNKLNDGRFQRTK
                     QKVQKQVEDIADDLIKLYAERSQLKGFAFSPDDENQIEFDNYFTHVETDDQLRSIDEI
                     KQDMEKDSPMDRLLVGDVGFGKTEVAMRAAFKAVNDGKQVAILVPTTVLAQQHYANFQ
                     ERFAEFPVNVDVMSRFKTKAEQEKTLEKLKKGQVDILIGTHRLLSKDVVFADLGLLVI
                     DEEQRFGVKHKERLKELKKKIDVLTLTATPIPRTLQMSMLGIRDLSVIETPPTNRYPV
                     QTYVMETNPSVIRDAMLREIDRGGQVYYLYNKVDTIEQKVSELKELVPEATIGYVHGQ
                     MSEIQLENTLYAFVEGEYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSSRIAYAYLMYRPDKSLTEVAEKRLEAIKGFTELGSGFKIAMQDLSIRGAGNILG
                     AAQSGFIDSVGYEMYSQLLEQAILEKQGKATQRQKSNSEVNLQIDAYLPSDYIGDQRQ
                     KIEIYKRIKNIDSRVNYQELQEELIDRFGEYPDVVAYLLEIGLLKSFLDQVFCHTVLR
                     RQHQVTVTFEPMACQIFLTQDYFEALSVTNLKAQITENKGKLAVVFNIQQKKEYEILE
                     ELISFAEKLKEIKARKAE"
     gene            7142..7414
                     /locus_tag="U1099_001060"
     CDS             7142..7414
                     /locus_tag="U1099_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEM4867389.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIQFGNKLLTVKVLEMKDSTKKEDALKMYEIISEKRIEKDEEI"
     gene            7401..7769
                     /locus_tag="U1099_001061"
     CDS             7401..7769
                     /locus_tag="U1099_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEM4867390.1"
                     /translation="MKKSKILQLNNAFIQSERKKTQHQLAERQQKNRFMGAILILVIF
                     LFMLPAYNLVGTYTNIQQQEKKLAELEKNYEELTKEQKQEAEMVAKLKNEEYAAKYVR
                     AKYQYSKEGEFVYNIPGLPK"
     gene            7892..9172
                     /locus_tag="U1099_001062"
     CDS             7892..9172
                     /locus_tag="U1099_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEM4867391.1"
                     /translation="MQKKLLVWLLPVLFGWQVVDSTEIPFELTAQEEYELTHTIYDQY
                     FQTIPQNPNVFQTENLYSDEELTIAGGQLQPNQHFSITDVLVNSKKELVFQIDDKGYI
                     LASRHLLFDDVIVTETTVEQTYWTKKGFTLLTSPIANEATEIKNDLQPYQAVMVSKIV
                     TTSLGDFAYVTDKGWIAVNNLSKTDNRVEAVQELLTNKYSKDTIGIYVKQLSTGQTAG
                     VNQEKLFYSASIAKLPILYYVQEQLNAGYIDLTTKVKYTAESISFPGAYVAGGSGSLS
                     KTPDNKDYSVEELINKTAKESDNVASNLLSYYVANKFDSNFYQVITAKTGSEWSMVTR
                     ETSAEIAGKMMESLYIQNGYVLESLLSTQFDNQRISKDISVPVAHKIGDADDVKHDVA
                     IVYAGSPFVLSIFTDKSNYDEITQIANDIYRILK"
     gene            9173..10441
                     /gene="tilS"
                     /locus_tag="U1099_001063"
     CDS             9173..10441
                     /gene="tilS"
                     /locus_tag="U1099_001063"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEM4867392.1"
                     /translation="MKDRFLKVTLDGHFFDKHNKVLVAVSGGLDSMNLFHLLYECKQV
                     LGIELGIAHINHGQREESVIEEKYLRQLAEESNVPFYLSYFEGVFSEEAARKWRYGFF
                     ATIMEKEGYTALVTAHHADDQAETVFMRLIRGGRLRHLSAIQPTQPFATGELIRPLLS
                     FKKADFEKLFHFEDCSNADFKYFRNRVRNDYLPKLKQENPKIEFTLNNFAVDTNNLFQ
                     ALRDLTQDLSVTDVMSFQQQTPAVQSYLLEEYLEKFPDLQLSRPQFNEVLHILRSKAN
                     YYHLLKNDYMLEKDYHRFQIYKIGPETDSQPEKIMIKSEGIFSYGSYIFSLNHPLEEA
                     DSILYFPTENPIVVRRRQAGDTILINGVNKKLRRWFIDNKISQKVRQNALIIEQNGEI
                     YGITNLVSSDLSKSVKNDIIKATLYIKMKE"
     gene            10449..10991
                     /gene="hpt"
                     /locus_tag="U1099_001064"
     CDS             10449..10991
                     /gene="hpt"
                     /locus_tag="U1099_001064"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEM4867393.1"
                     /translation="MLDKDIKKILVSEEEIVAKCKELGQILATDYADKNPILVGILKG
                     SIPFMAELMKHIDAHVETDYMVVSSYHGGTESCGTVKIIKDLDNSVAGRHIIFIEDII
                     DTGRTLKELKELFALRQAGSIKIATLLDKPEGRVVEIEPDYTCFTIPNEFVVGFGLDY
                     DENYRNLPYVGVLKEEVYTK"
     gene            11013..12983
                     /gene="ftsH"
                     /locus_tag="U1099_001065"
     CDS             11013..12983
                     /gene="ftsH"
                     /locus_tag="U1099_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEM4867394.1"
                     /translation="MNNQPNKGFIKNPFLVILVIATIVTAFQFFNAGQQVATQEISYS
                     QVVTELKNNNVSEITYQPNSSVIEITGKYKTEQEAKDELASSIKLFQVSSKVKYKNFK
                     SLILPSETNLSELQALANENGVKVTIKPESSNGLWLNIVFNLLPLIIAGVFFMMMMNQ
                     GGGARGAMNFGRNKAKALEQSNIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAAPAIIFIDEIDAVGRQRGVGMGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAADVDLKLVAQQTPGF
                     VGADLENVLNEAALVAARRNKTVIDASDIDEAEDRVIAGPSKKDRQVSAKEREIVAYH
                     EAGHTIVGLVLSNAREVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGG
                     RVAEEIIFNTQTTGASNDFEQATQMARAMVAEYGMSEKMGPMQYEGSHAMFGGQTTQK
                     HISEQTAYELDNEVRDLLNEARNKAADIIQSNRDTHKLIAEALLKYETLDSVQIKSLY
                     ETGKMPENIERDDEDVHPLSYEEVKEKINTKE"
BASE COUNT         4408 a         2189 c         2679 g         3835 t
ORIGIN      
        1 gagttctttc ccgctccctt tattttatac taaaaaatag cacctagctt atttttatat
       61 atttagtgat ggggaataaa tgacgttata tatattagct aatcctaatg ctggtagcca
      121 tactgctgaa catatcatat tccaaataaa agaaagttat ccacagcttg cagttaacat
      181 ttttatgaca gttggtcctg aggatgaaaa aagtcaaata gaggctattt taaaggagtt
      241 tgtcagtagt gaggaccaat taatgatttt aggtggagac ggcacactgt ctaaagcttt
      301 gcgtttttgg ccagctagtc taccgtttgc ttattatcca acaggatctg gaaatgattt
      361 tgctaaggca atgaatataa catcgctcta tagaagtgta gatgccattt tagagggaaa
      421 aacaagtcgg atatatgttt taaatagttc atacggaacg gttgtaaaca gtatggattt
      481 tggctttgca gctcaagtta tcaatggttc aacgaattca attttgaaaa aaaattctga
      541 acaaggtaaa acttgggaag ttaacttatc tattctttgg tattaaaaca ttattttcaa
      601 aacaagctat aaacttagaa ttaactcttg atgaaaaatc ttatcagtta gataatctct
      661 tttttatttc tgtagcaaat agtctttatt ttggtggagg aatcatgata tggccaacag
      721 caagtgctaa aaagaaggaa gtagatattg tttacttcaa aaatggaaat ttctaccaac
      781 gtctacaatc attgttagcc ttattaacga agaggcatga atcttctcat acaattcagc
      841 atttaacagg ggtagatgta gttttaaaat caaaagaaaa attattattg caaatagatg
      901 gagagacatg cactgcaaat gaggtaacgt taacctatca ggaaagaagt atgtatcttt
      961 aaggaggaag tatgtatcaa ttaggatcat ttgtcgaaat gaaaaagcct catgcctgtg
     1021 tcatcaaatc gaccggaaag aaggcaaata agtgggaggt tatccgtcta ggagcggata
     1081 ttaaaatccg ttgtaccaac tgtgaccatg tagtcatgat gagccggcat gattttgaac
     1141 aaaaaatgaa acaagttcta ccaagtgaag cctagttgac agtttgcaac tgctggttgc
     1201 gaatatttcc taaaaagtgc tagtcaactg ttcctttcta aactataata gacttagaaa
     1261 aaagaggagg aaatgatcat gaatcagtta gcacagcaaa ttcgaatttt acgcacagcc
     1321 aagaacctat cccaagatga gttggcagag aaactctata tttcccgtca ggctgtttcc
     1381 aagtgggaaa atggcgaagc aacgccagat attgacaaac tggttcagct agcagaaatc
     1441 tttggtgtca gtctggatta tctggtttta ggaaaagaac ctgagaagga aattgtggtg
     1501 gaacaacgag gaaaaatgaa tggttgggaa tttttaagtg aagaatccaa gcgaccagtt
     1561 gaaaataaag aaatttttct gtttatcctc tctatggcca tctgtatttt tgtagtttgg
     1621 atcttgacca tgattacaaa ataatggatt atttattcag cattattttt aacgaagctt
     1681 gctaatcggc aagctttttc tctacttttc tgctataata gtcatgattg aatttttaat
     1741 tggagagtaa aaaacatggc tttaacagca ggaatcgtcg gtttgccaaa cgttggtaaa
     1801 tcaactctat ttaacgcaat taccaaggca ggagcagaag ctgcaaacta ccctttcgcg
     1861 accattgatc caaacgtcgg catggtggaa gtgcccgatg agcgtttgca gaaattgaca
     1921 gaactcatca cccctaaaaa gacagtgcca acgacttttg aatttactga tattgccggt
     1981 atcgtaaaag gtgcttctaa aggtgaagga cttggaaata aattcttggc caatatccgc
     2041 gaagttgatg cgattgtcca cgttgtgcgt gcctttgacg atgaaaatgt tatgcgtgaa
     2101 caaggccgtg aagacgcctt tgttgatcct atcgcagata ttgatactat taacctagaa
     2161 ttgattttgg cggacctaga gagcatcaac aagcgttatg cgcgtgtaga aaaaatggcc
     2221 cgtacgcaaa aagacaagga ttctgtcgca gaatttgcag ttcttgaaaa aatcaaacct
     2281 gtcctagaag acggaaaatc tgctcgtaca gttgacttca cagatgaaga acaaaaaatc
     2341 gttaagcaac tcttcctctt gaccactaaa ccagtcctct atgttgccaa cgtcgatgaa
     2401 gacaaggtag cagatcctga gtctatcagt tatattcaga aaatccgtga ctttgcggca
     2461 acagaaaatg cagaagtggt ggtgatttct gcgcgtgctg aagaagaaat ttcagaactc
     2521 gacgatgaag acaagggcga gtttttagaa gcactcggat taacagaatc tggcgtggac
     2581 aaattaacac gtgcagccta tcacttgctt ggtctaggca cctactttac agcaggggag
     2641 aaggaggttc gtgcttggac cttcaaacgt ggtatgaaag ctcctcagtg tgcaggcatt
     2701 atccattcag acttcgaaaa aggttttatc cgtgccgtta ccatgtcata cgatgattta
     2761 atgacctacg gctctgagaa ggctgttaaa gaagcaggac gccttcgtga agaaggaaaa
     2821 gagtacgtgg ttcaagatgg ggacatcatg gagttccgct ttaatgtttg attgctaaac
     2881 tgtccttatt cttcgttgtt ttaccttgcc ataccatcag tattgtctgc ggtttcaacg
     2941 cctagactaa gaacagttta tcgcatcaaa gaggatgatt acttggttgg aaagcttttt
     3001 ccagcctttt ggtatttaag gagaaaaaat gacacgattg atcatcggtc tgggaaatcc
     3061 cggagaccgc tattttgaaa caaaacataa cgttggtttt atgctgctgg ataagattgc
     3121 taagcgtgaa aatgtgacct ttaatcacga taagattttc caagctgaca ttggcacaac
     3181 ctttattgac ggcgaaaaaa tctatctagt aaagccaacg accttcatga acgaatcagg
     3241 taaggctgtt catgccctga tggcttatta tggtctggat gcaactgata ttttagtagc
     3301 ttatgatgac ttagacatgg ctgtaggaaa aattcgtttc cgtcaaaaag gctcagctgg
     3361 tggccacaat ggtatcaaat ccattgtcaa gcatattgga acacaggaat ttgaccgtat
     3421 caagattggt attgggcgtc ctaaaggaaa aatgagcgtt gtcaaccacg tcttatcagg
     3481 atttgataca gaagaccgta ttgaaatcga cttagcacta gataaacttg acaaggctgt
     3541 caacgtatat ctcgaagaag atgattttga tacagtaatg agaaagttta acggataatg
     3601 aatatactcg atttacttca caagaacaag caaatcaatc agtggcagtc tggcttaaac
     3661 aagtcaactc gccaactatt attaggactc actggaacca gtaagtcatt agtcatggcg
     3721 actgcctatg atagtatggc tgagaaaata atgattgtga ctgctactca aaatgatgct
     3781 gagaaattag ttgcagattt agtagccatt attggaagtg agaatgttta caactttttt
     3841 acagatgata gtcctattgc cgagtttgtt tttgcatcaa aagaacggac tcaatcaagg
     3901 attgacagtt taaatttctt gacagattct actagttcag gaattttagt tgctagtatg
     3961 gctgcttgtc gggtcttatt gccaagtcct gagacttata aaggttctaa aataaagctt
     4021 gaagttggtc aagaaataga agttgacaaa cttgtaaata atcttgtcaa catcggctac
     4081 aaaaaagtgt ctcgtgtatt aacccaggga gaatttagcc aacgaggtga tattctagat
     4141 atttttgaca tgcaagcaga aactccttac cgaatagaat tttttggaga tgagattgat
     4201 ggcattcgta tttttgatgt tgacagtcaa aaatccttag aaaatctaga cgaaatctcg
     4261 atctctccag cttccgatat cattttgtct tcggaagact atagtagagc tagccaatac
     4321 attcagacag ctattgaaca atcgacccta gaggagcaac agtcttacct acgagaagtc
     4381 ttagctgata tgcagacgga gtaccggcat ccagatttgc gtaaattctt gtcctgtatc
     4441 tatgaacaat catggacatt gttggattac ttgccaaaga gttctccctt atttttggat
     4501 gattttcata aaatctctga taagcaggct caatttgaaa aagaaattgc agacttgttg
     4561 acagatgatt tacagaaggg taaaacagtg tcaagtttga aatattttgc ctcaacttac
     4621 gctgaattga gaaaatacaa acctgccacc ttcttttcaa gttttcaaaa aggtttaggc
     4681 aatgtaaagt ttgatgctct ttatcaattt acacagcatc ctatgcagga atttttccac
     4741 cagattccac tgttaaaaga tgaattgact cggtatgcga aatcaaacaa taccgttgtt
     4801 attcaagcga gttctgatgt gagtttacag actttacaga aaactttaca agagtatgat
     4861 attcatcttc cagtacatgc agcagataag ttagtagaag gtcagcaaca ggttacgatt
     4921 ggtcagttag cttcaggttt tcatttgatg gatgagaaac tagttttcat cactgaaaaa
     4981 gagattttca ataagaaaat gaagcgtaag acacgtagaa ccaatatttc taacgctgaa
     5041 cgtattaagg actatagtga attagcagtt ggtgactatg ttgttcacca tgttcatggt
     5101 atcggtcaat acctaggaat tgagactatt gaaatttcag gcattcatcg tgattattta
     5161 acggttcaat atcaaaactt cgatcgcatt tctattcctg tagaacaaat tgatcttctc
     5221 tccaaatact tagcgtccga tgggaaagct ccaaaagtta ataaactaaa cgatggccgg
     5281 ttccaacgaa ctaagcaaaa agttcagaag caagtggagg atattgcaga tgatttgatt
     5341 aagctttatg cggagcggag tcagctgaaa ggctttgcct tttcaccaga tgatgagaat
     5401 cagattgagt ttgacaacta ctttacacat gtggaaaccg acgaccaact tcgttccatt
     5461 gatgaaatca aacaagatat ggaaaaagac tctccaatgg atcgtttgct ggtaggagat
     5521 gtcggctttg gtaagacaga ggtagctatg cgcgctgctt tcaaagcagt caatgatggc
     5581 aagcaagtag ctattcttgt tcctacgact gttttagccc agcaacacta tgctaatttc
     5641 caggaacgat ttgcggaatt tcctgtcaat gtggatgtta tgagtcgttt taaaacaaag
     5701 gcagagcagg aaaaaacact tgagaagttg aaaaaaggcc aagttgacat cttgattggt
     5761 acccatcgcc ttctatcaaa agatgttgtt tttgctgatt taggtttact ggttattgac
     5821 gaagagcaac ggtttggtgt caagcataag gaacgcttga aggaattgaa aaagaaaata
     5881 gatgtcctaa ccttgactgc aactccgatt cctcggacat tgcaaatgtc catgctggga
     5941 atccgcgatt tatcagtaat tgagactcca ccgacaaatc gctatcctgt acaaacttat
     6001 gtgatggaaa caaatccttc cgttatccga gatgcaatgc ttcgtgagat agatcgcgga
     6061 ggtcaggttt actatcttta caataaagtt gacaccattg aacagaaagt gtctgaatta
     6121 aaagaattgg ttccagaagc tactatagga tacgtccatg gacaaatgtc ggagatccaa
     6181 ttagaaaata ctctctatgc ttttgtagag ggtgaatatg atattctagt gaccacaaca
     6241 atcatcgaaa caggtgttga tattccaaat gctaatacac tgtttattga aaatgcagat
     6301 catatgggac tgtcaactct ttatcaactt agaggacgtg ttggccgttc cagtcggatt
     6361 gcctatgcct atctcatgta ccgtccagac aagtccttga cagaagtagc tgaaaaacgt
     6421 ttggaggcaa tcaagggctt tacagaacta ggatcaggtt tcaagattgc catgcaagat
     6481 ttgtccattc gtggtgcggg aaatatttta ggtgctgctc agtcaggttt tattgattct
     6541 gtgggatatg agatgtattc acaattgctg gaacaagcca tcttggaaaa acaaggtaag
     6601 gcaactcaac gtcagaagag taattcagag gtcaatttac agattgatgc ctatctacca
     6661 agtgattata ttggtgacca gcgacaaaaa attgaaattt acaagcgtat aaagaatatt
     6721 gacagtcgtg tcaactatca agaattacag gaagaattga ttgaccgttt tggagagtat
     6781 cctgatgtag tagcctactt gcttgaaatc ggtctattga agtcattttt agaccaagtt
     6841 ttctgtcata cagttcttag acgacaacat caagtgacag taacattcga acctatggct
     6901 tgtcaaattt tcctaacgca agattacttt gaagctttat ctgtgacaaa tttgaaagct
     6961 cagataacgg aaaataaagg aaaactagct gttgtgttta acattcaaca gaaaaaagaa
     7021 tatgaaatat tagaggaatt gatttctttt gccgaaaaat taaaagaaat taaagcaagg
     7081 aaggcagaat aaatttctca aataccttga aaaatggtaa aataatgaac tgaggtaaag
     7141 tatgagacta gataaatatt tgaaagtatc ccgcattatt aagcgtcgaa cagttgcgaa
     7201 ggaagttgcc gataaaggac gaataaaagt caatgggatt ttggcgaaat cttcaacaga
     7261 tttaaaagta aatgatcagg ttgaaatcca atttggtaat aaactactca ctgtaaaagt
     7321 acttgaaatg aaagattcga ctaaaaaaga agacgcactg aaaatgtatg aaattatcag
     7381 tgaaaaaagg atagaaaaag atgaagaaat ctaaaattct acaactgaat aatgccttta
     7441 ttcagtcgga gcgtaaaaaa actcaacatc aattggcaga acgccaacaa aaaaatcgtt
     7501 ttatgggtgc aattctcatt ctagttatct ttctttttat gttgcctgcc tataatcttg
     7561 ttgggactta cactaatatt cagcagcagg aaaagaaact tgctgaattg gaaaaaaatt
     7621 atgaagaact gaccaaagaa cagaagcagg aagcagagat ggttgcaaaa ttgaaaaatg
     7681 aggaatacgc agcaaagtat gttcgggcta agtatcaata ctctaaagaa ggggaatttg
     7741 tctacaatat tccagggtta ccaaaatgac cgaatctatt tttaaaattg tagagcaatt
     7801 tttacagcat tctgatgaaa aattagaaga gttgaatcaa aagaatagag aattaaagtt
     7861 agaagaaaat gaatcgtaga aaggagggag tatgcagaaa aaactgctcg tatggttatt
     7921 gccagtttta tttggatggc aggtagtcga tagtacagaa ataccatttg aactcacagc
     7981 gcaagaagaa tacgaattga ctcatactat ctatgatcag tattttcaaa ctatccctca
     8041 aaacccaaat gtttttcaaa ctgagaatct ttattcggat gaagaactga ctatagcagg
     8101 tggacaatta cagccgaacc aacatttttc tattacggat gttctagtca atagcaaaaa
     8161 agaattggtt ttccaaatag atgacaaggg ttacattctt gcaagtaggc atctactatt
     8221 tgatgatgta attgttacag aaactactgt tgagcaaact tattggacaa aaaaaggttt
     8281 cacattgtta acctcaccaa ttgctaacga agctacggaa ataaaaaatg atttacaacc
     8341 ctatcaagct gttatggttt ctaaaattgt gacaacttcc ctaggtgatt ttgcctatgt
     8401 tacagataaa ggttggattg cagtaaataa tctttctaaa acggataata gagtagaagc
     8461 cgttcaagag ttgttgacta acaaatattc caaagatact attggtatat atgtgaaaca
     8521 attgtcaaca ggtcaaactg caggtgtaaa tcaagagaaa ctattctatt ctgccagcat
     8581 tgctaaacta ccaattcttt attacgttca agagcaactg aatgctgggt atattgattt
     8641 gactactaaa gtgaaatata cagccgaatc tatatccttc ccaggagctt atgttgctgg
     8701 aggaagtggt tcactgtcaa aaactcctga taacaaagat tattcagtgg aagaattgat
     8761 caataagact gctaaagagt cagataatgt agctagtaat ttactttctt attatgtagc
     8821 taataaattc gattccaatt tttatcaagt aataactgct aaaacaggta gtgagtggag
     8881 tatggttaca cgtgaaactt cagctgaaat cgcagggaaa atgatggaat ccttatatat
     8941 tcaaaatggc tatgtgctag aaagtttgtt atctactcaa tttgataatc aacgtatttc
     9001 gaaagacatc tcagttccag ttgctcataa aattggcgat gcagatgatg tcaaacacga
     9061 tgtagcgatt gtttacgcag ggtctccttt tgttttatct atttttacag ataaatcaaa
     9121 ttatgatgag attacacaaa ttgcaaatga tatttacagg attttaaagt aaatgaaaga
     9181 tagattttta aaagtgacac tggatggtca cttttttgat aaacataata aagtactagt
     9241 agccgtatct ggtgggttag attctatgaa cctattccat cttttatacg aatgcaaaca
     9301 agtgttaggg attgaacttg gaattgccca tattaatcat ggacagagag aagaatctgt
     9361 cattgaggaa aaatatctta gacagttagc agaagaaagt aatgttccct tttatctgtc
     9421 ctattttgaa ggtgtttttt cagaagaagc agcgcgaaaa tggcgttatg gattttttgc
     9481 tacaatcatg gagaaggaag gctacactgc actagtgaca gcccaccatg cagatgatca
     9541 agcagaaact gtctttatgc gattgattag aggagggcgt ttacgtcact tatcagcaat
     9601 tcaaccgact cagccttttg caacaggaga acttattcga ccacttttat cttttaaaaa
     9661 ggcagatttt gaaaagctat ttcattttga agattgcagc aatgcagatt tcaaatattt
     9721 tcgaaataga gtcagaaatg actatttacc aaagttaaaa caggaaaatc ctaaaatcga
     9781 atttactctc aataattttg cagttgatac aaataatcta tttcaagcgc taagagattt
     9841 gacacaggat ttatccgtta cagatgtgat gagttttcaa caacagacac ctgcggtaca
     9901 aagttatcta ttagaggaat atttggaaaa atttcctgat ttacagctct ctcgtccaca
     9961 atttaatgaa gtattacaca tcttgcgttc aaaagctaac tactatcatt tattgaaaaa
    10021 tgattacatg ttagagaagg attatcatcg ttttcaaatt tataaaatag gaccagagac
    10081 ggatagtcaa ccagaaaaaa tcatgataaa atcagagggt attttttctt acggttcata
    10141 tattttttct ctcaatcatc ctttggaaga agcggatagt attttatatt ttcctacaga
    10201 aaatccaatt gtagtgagga gaaggcaggc tggggatacc attttaataa acggagtaaa
    10261 taaaaaactc cgccgttggt ttattgataa taaaatctca cagaaagttc gacaaaatgc
    10321 gcttattatc gagcagaatg gagaaattta tggaattacg aatcttgtta gcagtgattt
    10381 gagtaaatca gtaaaaaatg atataatcaa agctacctta tatataaaaa tgaaagagta
    10441 gaacaactat gttggacaaa gatattaaga aaattcttgt ttcagaagaa gaaatcgttg
    10501 caaaatgcaa agaactcggt caaattctag caacagacta tgcagataag aatcctattc
    10561 ttgtaggaat tttaaaaggt tctatcccat ttatggcaga attaatgaaa catattgatg
    10621 cccatgtaga aacggattat atggttgttt ctagctacca tggtggtact gagagttgtg
    10681 gtaccgttaa gattatcaag gatttggata atagtgttgc aggtcgacat atcatcttta
    10741 tagaggatat tattgacaca ggccgcacat taaaagaatt gaaagaactc tttgctttgc
    10801 gtcaagcagg ttctattaaa attgctactc ttcttgataa accagaaggt cgtgttgttg
    10861 aaatcgagcc agactatact tgctttacta ttccaaatga atttgtagtt ggatttggct
    10921 tagattatga tgaaaattat cgtaatcttc cttatgttgg tgtactcaaa gaggaagttt
    10981 acacaaaata gaaaaaggtt tcctactata ttatgaacaa tcaaccaaat aaaggattta
    11041 taaaaaatcc ttttctcgtt attctcgtta ttgccactat tgttacagcc tttcaatttt
    11101 ttaacgctgg tcagcaagta gcaactcaag aaatcagtta ttcacaagta gtaactgaat
    11161 tgaaaaataa taatgtatct gaaattactt atcaaccaaa ttcaagtgtt attgaaatta
    11221 ctggtaagta caaaacagaa caggaagcta aagatgagtt agcttcatct attaagttat
    11281 tccaagtttc tagtaaagtt aagtataaaa actttaagtc actaatttta ccgtcagaaa
    11341 cgaatttgtc agagctacaa gctcttgcaa atgaaaatgg agtaaaggtt actattaaac
    11401 ctgaaagttc aaatggactt tggttaaata tcgtctttaa tttgttaccg cttatcattg
    11461 ctggtgtatt cttcatgatg atgatgaacc agggtggtgg agcacgtggt gcaatgaact
    11521 ttggacggaa taaagctaaa gctttggaac aaagtaacat caaggtacgt ttctcagatg
    11581 tagcaggtgc agaggaagaa aaacaagaat tagttgaagt tgttgaattt ttgaaggatc
    11641 caaaacgctt tactaaactt ggagcacgta ttccagcagg tgtactacta gagggtcctc
    11701 caggaaccgg taaaaccttg cttgccaaag cagtagcggg tgaagcgggt gtaccattct
    11761 tctccatttc tggttcagat tttgttgaaa tgtttgttgg tgtcggtgcc agccgtgttc
    11821 gttcattatt tgaagatgca aaaaaagcag caccagccat tattttcatc gatgaaatcg
    11881 atgctgtcgg tcgccaacgt ggtgtcggca tgggtggcgg taacgatgaa cgtgaacaaa
    11941 ccctcaacca actcttaatt gaaatggatg gttttgaagg gaatgaaggg attattgtca
    12001 ttgccgcaac caaccgtagt gatgttttag accctgccct tcttcgtcca ggtcgttttg
    12061 accgtaaagt attggttggt cgtccggatg ttaaaggacg tgaagcaatc cttaaagttc
    12121 atgcgaagaa taaaccacta gcagcagatg tagatttgaa attagtagca caacaaacac
    12181 caggatttgt tggtgcagat ttagagaatg ttcttaacga agcagccctt gtagctgccc
    12241 gtcgaaacaa gactgtcatt gatgcttcag atattgacga agcagaagat cgtgtcattg
    12301 caggtccttc taagaaagac cgtcaagttt cagccaaaga acgcgaaatc gttgcctacc
    12361 atgaagcagg tcatacaatt gttggtctcg tactatcaaa tgcacgtgaa gttcacaaag
    12421 taacaattgt tccccgcggt cgcgcaggtg gatacatgat tgcccttcct aaagaagatc
    12481 aaatgcttct ttctaaagaa gatatgaagg agcaattggc tgggcttatg ggtggtcgtg
    12541 ttgcggaaga aattattttt aatacacaga ccacaggtgc ctcaaatgac tttgaacaag
    12601 ctacacagat ggctcgtgct atggttgcag aatatggtat gagtgaaaaa atgggaccaa
    12661 tgcagtatga aggcagtcat gcaatgtttg gtggacaaac aactcaaaaa catatttctg
    12721 agcaaactgc atatgagtta gataatgaag tacgtgattt gttaaatgaa gctcgaaata
    12781 aagctgcaga tattattcaa tcaaaccgtg atacacacaa actgattgca gaagcattgc
    12841 ttaaatacga aaccctagac agtgttcaga tcaaatctct ctatgaaaca ggcaaaatgc
    12901 cagagaatat tgaacgtgat gatgaagatg ttcaccccct ttcttatgaa gaggtaaaag
    12961 agaaaattaa tacgaaagag tagtataaaa atactcttgg caagacattc cctcactctc
    13021 aaccactaaa ataaccacaa atccttcgct aagtacataa tcttggcgag ggcttttttt
    13081 acaaaaaagg gcatttacaa gaaaaacgaa t
//
LOCUS       DASFSW010000051        13096 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.051, whole genome shotgun sequence.
ACCESSION   DASFSW010000051 DASFSW010000000
VERSION     DASFSW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.051"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1013)
                     /locus_tag="U1099_001066"
     CDS             complement(<1..1013)
                     /locus_tag="U1099_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867395.1"
                     /translation="MNTKKWRTSLLIPGIVLFGTVALVNNVSAQEVKNTIISAKQPDG
                     RQATSKAVNVKIPAVVRLFGRELLENEFKFELREANSEKLIDTTQNTKEGQVSFKNLS
                     FNKPGEYWYTISEVKNELSGVEFDSKYIVAKITVEDRNNGQLQAMIEFIADDNVFNNF
                     YTPAPAAASLSIKKVLEGRTLNTGEFEFVLKNEKGDEIEKVSNQADGSVNFSALTFTK
                     EGTYTYTVSEVDGGLGDIIYDKSDIKATVTVKDNNHGQLVSTVTYENSDQIFENILNP
                     GKLITPTTDSVITDNEVSKEAMTDKEKGNIEPPKEQIATEEKDNIEASEKQMPSIVND
                     MVVT"
     gene            1323..2321
                     /locus_tag="U1099_001067"
     CDS             1323..2321
                     /locus_tag="U1099_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM4867396.1"
                     /translation="MKVLLYLEGKTVLEKSGIGRALQHQMEALDMAGIPYTTDLLGDY
                     DVIHINTYGPRSWLLLHAAKRSGKKVIMHGHSTKEDFQNSFIGSNTLAPFVGKYLASM
                     YQKADFVITPSEYSKKLIQSYGVTTPIIAVSNGIDLDKYKKDERKEAIFREHFKIEEG
                     QPVVVCAGLYFRRKGIEDFVKVAEKMPHVRFIWLGSINKWLIPSYIRKIVEGDHPANV
                     EFPGYFKGAVFQGAMSGADAFFFPSYEETEGIVVLEALASHQHVVLRDIPVYDGWVDE
                     NVAELGHTVDDFVDSIQKILDKQVDKREAGYQVAVSRSIDNVSHQLVDAYRQVLEM"
     gene            2322..3635
                     /locus_tag="U1099_001068"
     CDS             2322..3635
                     /locus_tag="U1099_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM4867397.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKLGHTVFIFTTTDED
                     VNRYEDWDIIRIPSVPFFAFKDRRVAYAGFTDALKIASRYKLDIIHTHTEFSLGILGK
                     MIAKELDIPVVHTYHTQYEDYVHYIAKGRIIRPGMVKYLVRAFLKDLDGVICPSEIVE
                     DLLMSYNVPISKRVIPTGIDLAKFDRPEIGKDETDELREQLGIASDETMLLSLSRISH
                     EKNIQAIVQAMPKILLENPKVKLVIVGGGPYTDDLQEMIAELNLDEHVQLTGMIAPSD
                     TALYYKAADFFISASTSETQGLTFLESLASGTPILAHSNPYLKNVVTDKMFGTLFVRE
                     QELADAVVEAIVATPPMNPYVLDKKLYEISSTNFGRKVFEYYLDLKISYDFRKEHTNQ
                     DSVAEALIKEAIYLPQKVVVKSTDTTAKILKKSVEQVKSIRNFFE"
     gene            3650..4024
                     /locus_tag="U1099_001069"
     CDS             3650..4024
                     /locus_tag="U1099_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867398.1"
                     /translation="MEKLRKESWKNLVLATLIILSMYAGIYLIAFHLISDTMLSKELV
                     VGNILLYTPIVLFLTGLLYGFFHGFSPSLLALVLLLYPVTIFFFKEWIILYQIVYTLC
                     ALIGNGVGALLFSLRKKMRKNP"
     gene            4474..4659
                     /locus_tag="U1099_001070"
     CDS             4474..4659
                     /locus_tag="U1099_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEM4867399.1"
                     /translation="MNRLIMTKQGRYYDETPYTLEHKMAENIWWLIELADRLDIDIQK
                     EMETFLTQKEELLGIKK"
     gene            4677..6626
                     /gene="thrS"
                     /locus_tag="U1099_001071"
     CDS             4677..6626
                     /gene="thrS"
                     /locus_tag="U1099_001071"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEM4867400.1"
                     /translation="MIKITFPDGAVREYQAGVTTFEIAESISKSLAKKALAGKFNGKL
                     IDTTRAIEEDGTLEIVTPDHEDALDILRHSAAHLFAQAARRLFPDIKLGVGPAIQDGF
                     YYDTDNAAGQISNEDLPRIQEEMMKIVKENFPSERREVTKEEAIEIFKNDPYKLELIQ
                     EHSDDEGGLTIYTQGEYIDLCRGPHVPSTGRIQIFELLNVAGAYWRGKSENPMMQRVY
                     GTAWFDKKDLKAYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRT
                     LERYITDKELTSGYQHVYTPPLASVELYKTSGHWEHYSEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVYKNHVRSYRELPVRIAELGMMHRYEKSGALTGLQRVREMTLNDGHIFVTP
                     EQIQEEFKKALQLIIDVYADFNLNDYRFRLSYRDPEDKEKYYDNDEMWENAQRMLKGA
                     MDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFGLTYIGADG
                     EEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPTQVTMIPISVEAHLDYAWKVA
                     KELQDRGVRVHVDERNEKMQYKIRQSQTSKIPYQLIVGDKEMEDNAVNVRRYGSKATQ
                     TQSVAEFVDHILADIARKSRPAEAE"
     gene            complement(6698..7357)
                     /locus_tag="U1099_001072"
     CDS             complement(6698..7357)
                     /locus_tag="U1099_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM4867401.1"
                     /translation="MNYVLELLKPSTFQLLWNGLKLTLYISSISVILSILFGMILAIM
                     RNGKNPIFKWIATIYIEFVRNVPNLLWIFTVFLVFQMKSTPAGITAFTIFTTAAMAEI
                     IRGGLNAIGPGQTEAGLSQGFTPVQIMVYIIMPQAIRKMLPAIISQIVTVIKDTSFLY
                     SVIALQELFGSSQILMGRYFEAEQVFALYLMVALIYFSINFAISSLSRYVAKSWASSI
                     E"
     gene            complement(7372..8070)
                     /locus_tag="U1099_001073"
     CDS             complement(7372..8070)
                     /locus_tag="U1099_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM4867402.1"
                     /translation="MTVLTTSPYAWENWVSYFKDFPTFFQGFLFTLAISVGAFIMAMF
                     LGIVFGSLSSSKNKVLKIIARVYVEYYQNTPLLVQFMIVYYGLPLISNYVLMPSIYWT
                     AVICVGLYHGAYIAEVIRAGIEAVPTGQTEAALSQGFTQSETMRIIILPQAIPTILPP
                     LTNQVVNLIKNTATVAIISGADIMFMTKSWSAMNANYIPAFAGAAFLYFIMCFPVASW
                     GRRIEEKNKSAFSH"
     gene            complement(8085..8924)
                     /locus_tag="U1099_001074"
     CDS             complement(8085..8924)
                     /locus_tag="U1099_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM4867403.1"
                     /translation="MDKKKINIFFAIRLLVFGLILFVFAKQPVAQTETPASITNSDQV
                     QAIIDRGVLRVGVKQDVPNFGYKNPDTGEFEGMEIDIARKIADELGVDIEFTPVTAQT
                     RGPLLDNGQVDMVIATFTITEERKLLYNFTTPYYTDAVGFLVNKDSGIKTFTDLDGKT
                     IGVAQGSITRTLISKLADKYGISVNFAELGSYPELSVSLRAHRTDAFSVDQSILSGYI
                     SSKSELMDFSFSASDYGIVTKLSNKDLNNYLNSLVEKWTADGSLQSIYDANGLKPVTE
                     TDE"
     gene            complement(8935..9696)
                     /locus_tag="U1099_001075"
     CDS             complement(8935..9696)
                     /locus_tag="U1099_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM4867404.1"
                     /translation="MALVEFKDVNKYYGDYHALRNINLEFEPGQVVVLLGPSGSGKST
                     LIRTINGLEAIDEGTLIVNGHDISSTSIKELVSLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKATAEKTAQKYLEFVNLWDRKDSYPAMLSGGQKQRVAIARGLAMKPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIVVTHEMGFAREVADRIIFMAEGEVL
                     VDTTDVNGFFDNPTEPRAQQFLSKIINHESDKVKL"
     gene            9890..10594
                     /locus_tag="U1099_001076"
     CDS             9890..10594
                     /locus_tag="U1099_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4867405.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTREGYEVVTAFDGREALEVFEAE
                     FPDIVILDLMLPELDGLEVARTIRKTSNVPILMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELQARVKALLRRSELAETQTNIESTGTPELVIGDLVILPDAFVAKKHGKELELT
                     HREFELLHHLAKHLGQVMTREHLLETVWGYDYFGDVRTVDVTIRRLREKIEDTPSRPE
                     YILTRRGVGYFIKGND"
     gene            10587..11936
                     /gene="vicK"
                     /locus_tag="U1099_001077"
     CDS             10587..11936
                     /gene="vicK"
                     /locus_tag="U1099_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEM4867406.1"
                     /translation="MINQLRYLMTTAEFWFVVILIGFLIALTVLLIENYRDNKQIKQL
                     NQKVNALIEGNYADVLDMRGSPEITDMANSLNDLSEVIRLTHDNLEQEKTRLTSILSY
                     MSDGVIATDRIGRIIMINDMAQKQLGLSSQKQEQYHLLEVLDLSDRYTLRDLLAQTPE
                     IVIDHTNENEEFLTLRANFATIRSESGLISGLVVVLHDMTEQAKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALTESVAPSFVKVSLDETNRMMRMITDLLSLSRIDNQVG
                     QIDVELINFTAFVTFILNRFDQMKNTDSDKVYTIVRDYQISPIWVEIDTDKMTQVLDN
                     ILNNAIKYSPDGGTITFSMKTTDSQLIVSVSDEGLGIPKTDLPRIFDRFYRVDKARSR
                     AQGGTGLGLAIAKEIVKQHKGFIWAKSEYGHGSTFTIVLPYSKDIALDEWDDSDEEE"
     gene            11943..12746
                     /locus_tag="U1099_001078"
     CDS             11943..12746
                     /locus_tag="U1099_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM4867407.1"
                     /translation="MIGKGFNYSILASGSSGNCFYLETDKKKILVDAGLSGKKVTSLL
                     AEIDRKPEDIDAILVTHEHSDHIHGIGVLARKYGMDIYANELTWQAMESKLGKIDVAQ
                     KHIFELGAMKTFGDLDIESFGVSHDAACPQFYRFMKDDKSFVMLTDTGYVSDRMVGIV
                     ENADAYLIESNHDIEILRSGSYSWNLKQRILSDKGHLCNEDGADAMIRSLGNRTKKIY
                     LGHLSKENNIKELAHMTMVNQLAQADLGVGVDFQVYDTSPDTATPLTKI"
BASE COUNT         3829 a         2425 c         2973 g         3869 t
ORIGIN      
        1 gttacgacca tgtcgttcac aatgcttggc atctgttttt cagaggcttc aatattatcc
       61 ttctcttcag tagctatttg ctctttaggg ggttcgatat ttcccttctc tttatcggtc
      121 attgcttcct tagagacttc attatcagta ataacgctat ccgtggttgg cgttattaac
      181 ttcccaggat tcaaaatatt ctcgaagatt tgatcgctat tttcataagt cactgttgag
      241 actagttgtc cgtgattgtt atctttcaca gtgacagtgg ccttaatatc tgatttgtca
      301 tagataatat cgccaagtcc accatcaact tctgaaacag tgtaggtata ggttccctct
      361 tttgtaaatg ttagggcact aaagtttaca gaaccatctg cttgattgct taccttttcg
      421 atttcatcgc ctttttcatt ttttaaaaca aattcgaatt caccggtgtt taaggtacgt
      481 ccctcgagga cttttttaat cgaaagacta gcagcagctg gagcaggtgt atagaaattg
      541 ttaaagacat tgtcatcagc aataaattca atcattgcct gtaattgtcc gttgtttcga
      601 tcttctacag ttatttttgc tacaatatat ttcgaatcaa actcaacacc actaagctca
      661 ttttttactt ctgaaattgt ataccaatat tcgccgggct tattgaatga tagattttta
      721 aaactaactt gaccctcttt tgtattttga gttgtatcaa taagtttctc actattcgct
      781 tctctaagct caaatttaaa ttcattttct agaagctcac gaccaaatag tcgtactact
      841 gctggtattt tgacattaac cgcctttgaa gtagcctgtc tcccatcagg ttgttttgcg
      901 ctgatgatgg tattttttac ttcttgtgcc gatacattat tcactagagc aacagttcca
      961 aataatacta ttcctggtat taggagcgat gttctccatt tcttagtatt cattatcctt
     1021 tttcctttct caaagcatga tttatgtagc taatctgtgt cgttataaca tgcaaatttt
     1081 acatctaaag tcctcaataa attttgaacg cttagtgcca gaatgtgcac catgcgtaaa
     1141 ttataacatc ttctaaaaaa cattcaagct gtccttctgt ttttaaaatc tgttttatat
     1201 gttttttatg taatcatata tttgggatta tattctttag tattggcttt aattgataca
     1261 aaattcgtgt agaatacagt ttaaaatatg aaaaaaatgt aaaaaagggc tataatggta
     1321 ttatgaaagt actattgtat ttagaaggta aaactgtttt agagaaatca ggaattggcc
     1381 gggcacttca acaccagatg gaagcgctag atatggcagg cattccttat acaacagacc
     1441 tgttgggaga ttacgatgtc attcatatca atacctatgg tccacgctcc tggttattgc
     1501 tacatgcagc caaacggagt ggaaaaaagg taatcatgca tggacattcc acgaaggaag
     1561 attttcaaaa ttcctttatc ggttcgaata ccctagctcc ttttgtaggg aaatatctag
     1621 caagcatgta ccaaaaggca gactttgtca tcactccttc agagtattca aaaaaactca
     1681 tccagtctta cggtgtgaca acgccaatta tagccgtttc gaatggtatt gatttagata
     1741 aatataagaa ggatgaacgc aaggaagcaa tctttcgaga gcattttaaa attgaagaag
     1801 ggcagccggt tgttgtctgt gcaggactct acttccgacg taaaggaatt gaagactttg
     1861 tcaaggtagc cgaaaagatg ccgcatgtcc gcttcatttg gctcggttca atcaataagt
     1921 ggctgattcc gtcatatatt agaaaaattg ttgagggaga tcatccagcg aatgtcgagt
     1981 ttccaggtta ttttaaaggt gctgtttttc aaggtgcaat gagcggagcg gatgcctttt
     2041 tcttcccatc gtatgaggaa acagaaggga ttgttgttct ggaagctctt gctagtcatc
     2101 agcatgttgt tctaagagat attccagttt acgatggttg ggttgacgaa aacgttgccg
     2161 aattgggaca tacagtggat gattttgtcg attctattca gaaaatctta gacaagcagg
     2221 ttgataaacg tgaggcgggc taccaggttg cggttagtcg ctcgattgac aatgtttccc
     2281 accaattggt ggatgcctat cgacaagtat tggagatgta aatgcggatt ggattgttta
     2341 cggataccta ttttccacag gtttcaggag tcgcgacttc gattcggact cttaagacag
     2401 agttggagaa attgggacat acggtattta tttttacgac gacagatgag gatgtgaatc
     2461 gctacgaaga ttgggacatt atccgaattc ctagcgtacc tttttttgcc tttaaagatc
     2521 gtcgagtggc ctacgcaggt tttacagatg ccttaaaaat tgcaagtcgc tataaactag
     2581 atattattca tactcataca gaattttctt tggggatttt ggggaaaatg attgccaagg
     2641 aactagatat tccggtggta catacttatc acacccagta tgaagactac gtacactata
     2701 ttgccaaggg taggattatt cgtcccggga tggttaaata tttagtacgg gcatttttaa
     2761 aagatttgga tggggtaatt tgtcctagtg agattgttga agatctttta atgagttaca
     2821 atgttccgat ttctaaacga gtaattccga caggaattga cttggcaaag tttgatagac
     2881 cagaaattgg caaggatgaa acagatgagt tgcgggaaca gctaggaatt gctagtgatg
     2941 aaacgatgtt gttaagtctg tctaggattt ctcacgagaa gaatatccaa gcaatcgttc
     3001 aggcaatgcc aaaaattttg ctagaaaatc caaaggtgaa gttggtcatt gtcggaggtg
     3061 gaccttatac agatgatctt caggaaatga tagcggagct taatctagat gaacacgtcc
     3121 agttgacggg aatgattgca ccaagtgata cagcacttta ttataaagct gcggatttct
     3181 ttatttctgc ttcgaccagt gaaacacagg gactaacttt cttagaaagt ttagcttctg
     3241 gaactcctat tttggcacat agcaatccct acttaaaaaa tgttgtaaca gataagatgt
     3301 ttgggacact cttcgttcga gaacaggagt tggcagatgc cgtcgttgag gcgattgttg
     3361 ctacaccacc aatgaatccg tatgtgcttg ataagaagct ctatgagatt tcttctacga
     3421 attttgggcg caaggttttt gaatattatc tagatttgaa gatttcgtat gattttagaa
     3481 aagaacatac gaatcaagat agcgtagcag aggcattgat aaaagaggct atttacctac
     3541 cgcaaaaagt agtagttaaa tcaacagaca cgacagcaaa aatattgaaa aaatcagtcg
     3601 aacaagtaaa atcaatccgg aatttctttg aataatttgg aggtttcaaa tggaaaagct
     3661 tcggaaagag agttggaaga atctggtttt ggcaactctt attattctgt ccatgtatgc
     3721 aggtatttat ctgattgcct ttcatctgat tagtgatact atgctttcta aggaactggt
     3781 ggttgggaat atattgctct atactcccat tgtactattc ctaacaggtc ttctttatgg
     3841 attttttcac ggtttttctc ctagccttct tgccttggtg ttactgcttt atcctgtaac
     3901 catctttttc tttaaggaat ggattattct ctatcagatt gtctacacct tatgtgctct
     3961 gattggaaat ggggtaggag ctctgctatt ttctctaaga aagaaaatga ggaaaaatcc
     4021 ttgatatatc ggaaaatcct tgttataata gcttttagag acttatcaat tctaggaaat
     4081 ctttgagaga gtgtgcggtg ggtgcaagca catagaggat aggattgaca ctactcagta
     4141 tgattttatg caaacataaa agggtggttc ccttatcacc aatctgaggt agtcaggctt
     4201 tttagcgtga ctgcgaatga aggtggaacc acgttacgac gtccttttgt gagggcgtcg
     4261 tattttcttt tttgacaaaa caaggagatg aagttgacca gcattccaca agaaacgatt
     4321 gatcgttgcc tagccgttcg caaggcttac agagagttgg aactcaaaca ccacgacaaa
     4381 gagtggtcca ttgaggagtg aaacagtctg gggatagact gtttcagctc aacaacttaa
     4441 aataaagact tgttgaccaa tgatattggc aacatgaacc gtctgattat gaccaagcag
     4501 ggtcgttact atgatgaaac gccttatacc ttggagcata aaatggctga aaatatctgg
     4561 tggttgattg aactggctga ccgcctggat attgatattc aaaaagaaat ggaaaccttt
     4621 ttaactcaga aagaagagtt attggggatt aaaaaataaa taaaaaggag ataaacatga
     4681 ttaaaatcac tttcccagat ggtgctgttc gtgagtacca ggctggcgtg acaacttttg
     4741 aaattgcgga gagtatcagc aagtctttgg cgaaaaaggc gcttgctggt aagttcaatg
     4801 gtaaattgat tgacaccact cgtgccattg aagaggatgg cacgcttgaa atcgtaacac
     4861 ctgaccatga ggatgccttg gacatcttgc gtcactcagc agctcacttg tttgcccaag
     4921 cggctcgtcg ccttttccca gacatcaagt tgggggttgg tcctgctatt caggatggtt
     4981 tctactatga cacggacaat gcggctggtc agatttcaaa tgaagacctg ccacgcattc
     5041 aggaagaaat gatgaagatt gtcaaggaaa acttcccttc agaacgtcgt gaggtgacca
     5101 aggaagaagc tattgagatt ttcaaaaacg atccttacaa gttggaattg attcaagaac
     5161 actctgatga cgagggcggt ttgaccattt atacccaggg cgaatacatt gacctctgcc
     5221 gtggcccaca cgttccgtca acaggtcgta tccagatttt tgaattgctc aatgtggctg
     5281 gtgcttactg gcgtggtaag agcgaaaatc caatgatgca acgggtttat ggtacagctt
     5341 ggtttgacaa gaaagacctt aaggcttacc ttcaaatgcg tgaagaggca aaagaacgtg
     5401 accaccgtaa acttggtaag gaattggatc tcttcatgat cagccaagaa gttggtcaag
     5461 gcttgccatt ctggttgcca aatggagcga ccatccgtcg taccttagag cgttacatca
     5521 cagataaaga gttgacttca ggttaccagc acgtttatac gccaccattg gcttctgttg
     5581 aactctataa gacttcgggt cactgggagc attattcaga agatatgttc ccaactatgg
     5641 acatgggcga cggtgaagag tttgttcttc gtccgatgaa ctgtccgcac cacattcaag
     5701 tttataaaaa tcatgtgcgt tcataccgtg aattgccagt gcgtatcgcc gagcttggta
     5761 tgatgcaccg ctatgaaaaa tctggtgcat tgactggttt gcaacgggtg cgtgagatga
     5821 ccctcaacga cggtcatatc tttgtcacac ctgagcaaat ccaagaagag ttcaaaaaag
     5881 ctctccagtt gatcatcgat gtgtacgcag atttcaacct aaatgactac cgtttccgcc
     5941 tgtcttatcg tgacccagag gacaaagaga agtactatga caacgatgag atgtgggaaa
     6001 atgcccaacg catgttgaaa ggtgccatgg atgaaatggg cgtagactac tttgaggcag
     6061 agggtgaagc tgccttctac ggtccgaaat tggatattca agtgaagact gcccttggta
     6121 acgaagaaac cctgtcaact attcagttag acttcctctt gccagaacgc tttggtttga
     6181 cttatatcgg tgctgatggt gaggagcacc gcccagtcat gattcaccgt ggtgttatct
     6241 caaccatgga acgcttcacg gctatcttga ttgaaaccta caagggtgcc ttcccaactt
     6301 ggttggctcc aacccaagtg actatgattc cgatctctgt ggaagctcac ttggactacg
     6361 cttggaaggt tgctaaggaa ttgcaagacc gtggcgttcg cgtacacgtg gatgagcgga
     6421 acgagaagat gcaatacaag atccgtcaga gccagaccag caaaattcca taccagctca
     6481 tcgtcggtga caaggaaatg gaagacaatg ctgtcaacgt ccgtcgctac ggaagtaagg
     6541 caacgcaaac tcagtctgtc gcagaatttg tggaccatat cctagcagat attgcccgca
     6601 aatcacgtcc agctgaagct gaataaataa agtaagaagt ccgaaaaggc ttcttttttg
     6661 acagcaaaaa ctcccaacca actggctggg agaaatttta ttctatactg ctcgcccaag
     6721 acttagccac ataacgtgac aggcttgaaa tggcaaagtt gatgctaaag taaatcaagg
     6781 ctaccatgag atagagtgca aaaacttgtt ctgcctcaaa gtagcgtccc atgaggattt
     6841 gactcgaacc gaagagttct tgcagggcga tgacggagta gaggaaacta gtgtccttga
     6901 tgaccgttac aatctgcgag atgatggctg gcagcatctt gcgaatggcc tgcggcatga
     6961 tgatatagac catgatttgc actggggtaa aaccctgtga caagccagct tcggtctggc
     7021 ccggtccgat ggcgtttaga ccaccacgga taatctcggc catggctgcc gtggtaaaaa
     7081 tagtaaaagc agtaatacca gcaggcgttg acttcatttg gaagactaag aagacggtga
     7141 aaatccagag caagtttggt acatttcgca caaactcgat atagatagtt gcaatccact
     7201 tgaaaattgg attttttcca ttgcgcatga tggcgagaat cattccgaaa agaatggaca
     7261 ggattaccga aatgcttgag atatagagtg ttaacttcaa accattccac aagagttgga
     7321 aggttgaagg tttgagtaat tctaaaacat aattcatcgt tcaacctcct tttaatgtga
     7381 aaaggcagac ttattctttt cttcaatgcg acgtccccag cttgcaacag ggaagcacat
     7441 gatgaagtaa aggaaagcag caccagcaaa ggctgggatg tagttggcat tcatggcaga
     7501 ccaagatttt gtcataaaca taatatcagc tccagagata atggcaacag tagccgtatt
     7561 cttaattaag tttactactt ggttggtcaa aggtggaaga atggtcggaa tggcctgtgg
     7621 caagatgatg attcgcatgg tttcagactg agtaaatcct tgtgagagtg cggcttcagt
     7681 ttgcccagta ggcacagcct caatccctgc acgaatgacc tctgcgatat aggcaccgtg
     7741 atagagtccg acgcagataa cagctgtcca gtagatggac ggcatgagaa cataatttga
     7801 aatcagcgga agtccataat aaacaatcat gaactgaacc aaaagcggcg tgttctgata
     7861 gtattcaaca tagacacgcg cgatgatttt taaaacttta tttttacttg aagagagact
     7921 accaaagaca attcctaaaa acatggccat gataaaggca ccgacagaaa tggctaaggt
     7981 aaagagaaaa ccttggaaaa aggttggaaa atctttgaaa taactgaccc aattctccca
     8041 agcgtaagga ctagttgtta aaactgtcat attgtttcac ctttctattc gtcagtttct
     8101 gtgactggtt tgagaccatt ggcatcatag atgctttgta ggctaccatc ggcagtccat
     8161 ttttctacga gactatttaa gtagttgtta aggtctttgt tagataattt agtaacaatt
     8221 ccatagtcag aagctgagaa gctaaagtcc atgagttcag atttagagct gatataccca
     8281 gaaaggatag attggtctac tgaaaatgcg tctgtacggt gagcacgtag cgagacagag
     8341 agttctggat aagaaccaag ctctgcaaaa ttgacagaga taccatattt gtctgctagt
     8401 ttagagatga gggtacgtgt aatggaaccc tgagctacac cgattgtctt tccatccagg
     8461 tcggtaaatg tcttaattcc actatcctta ttgaccaaga agccaacggc atctgtgtag
     8521 taaggagttg tgaaattgta gaggagttta cgttcctcag tgatagtgaa ggttgcgatg
     8581 accatatcca cttgaccgtt atctaagagt ggtcctcggg tttgcgctgt aacgggagta
     8641 aattcgatat ctacacctaa ttcatctgca attttacgag caatatcaat ctccattccc
     8701 tcaaattcac cagtatcagg gttcttgtag ccaaagtttg ggacgtcctg tttcactccg
     8761 actcggagaa cgcctctatc gatgatagct tgaacttgat cactattggt aatggatgcg
     8821 ggtgtttctg tttgagctac tggttgtttt gcgaaaacga aaagaataag tccaaagacc
     8881 aataatctaa ttgcaaagaa aatgttgatt tttttcttat ccatagactt cctcctagag
     8941 ttttactttg tcagattcgt ggttgataat cttgctgagg aattgttggg cacgaggctc
     9001 tgtcggattg tcaaagaagc catttacatc tgttgtatca actaaaactt ctccctcagc
     9061 catgaagatg atgcgatcag ctacttcacg ggcaaagccc atttcgtgtg ttacgacaat
     9121 catgttcatc ccgtcacgtg ccaatttttg cataactgca agtacatcgc cgatagtttc
     9181 tgggtccaaa gcagatgttg gttcatcgaa gagtagaagc tcaggtttca tagctaagcc
     9241 gcgagcgatg gcgacacgtt gtttttggcc tccggatagc atagcagggt aggaatcctt
     9301 ccgatcccag aggttaacaa actcgagata cttttgagca gttttttcag ccgtagcttt
     9361 atcgattccc aaaaccttaa tcggtgccaa agtaacattt tctaacacgg ttttgtgtgg
     9421 gtaaaggttg aaatgctgga aaaccatgcc aacttctttg cgaagtgata cgagctcttt
     9481 gatagaagta gaggaaatat catgtccatt gacgatgagc gttccttcat caattgcctc
     9541 taaaccgtta atcgtgcgaa tgagagtaga ttttccggat ccagaaggtc caaggagaac
     9601 gacaacctgt ccaggttcga attccaagtt gatattgcgt agggcgtggt agtcgccata
     9661 gtatttgttg acatctttaa attctactaa agccataaaa ttctccttgc atagtgtttt
     9721 ttaggtattc ccatcttagc attgcctata tacaatgtca agtaaattct gacaatttaa
     9781 tgtattgtat gtaataaaat atgacatagc tgtgtcagat ggttttgaaa tatgaatttt
     9841 ttaggtaagt ctgatataat agaagaaata aagcaaaggt aagatactaa tgaaaaaaat
     9901 attaattgta gatgatgaaa aaccaatctc agatattatt aagtttaata tgacgcgtga
     9961 gggatatgaa gttgtgacag ctttcgatgg acgtgaagcc ttggaagtgt ttgaggctga
    10021 gtttcctgac attgtcattt tggacttgat gttgccagaa ttggacggac tagaggttgc
    10081 tcgaaccatt cgtaagacca gcaatgttcc aatcttgatg ttatctgcta aagatagcga
    10141 atttgataag gttatcgggc ttgaaatcgg ggcggatgat tatgtgacca aacccttctc
    10201 taatcgcgaa ttgcaggcgc gtgttaaggc tcttcttcgt cgtagtgaat tggcagagac
    10261 gcagacaaat attgagtcaa caggaactcc agagttggtg attggcgatt tggtcattct
    10321 gcctgatgcg tttgtcgcta agaagcatgg caaagagctg gagctgaccc atcgtgagtt
    10381 tgaattgctc caccatctgg ccaaacactt aggtcaggtt atgactcgag aacatctatt
    10441 ggaaacagtt tggggttatg attactttgg tgatgtccgc acggtggatg taacgattcg
    10501 tcgtctgcgt gagaaaattg aagatacacc aagcagacca gaatacattc ttactcgtcg
    10561 cggagtggga tattttataa aaggaaatga ttaatcaatt acgttattta atgaccacgg
    10621 cagaattttg gtttgttgtc attttgattg gctttctaat tgctctgacg gtccttttga
    10681 ttgaaaacta tcgggataat aagcaaatta aacaactcaa tcaaaaagtc aatgccttaa
    10741 ttgaggggaa ttatgcagat gtgttagaca tgaggggaag tcctgaaatc acagatatgg
    10801 cgaattctct taatgatctg tccgaagtca tccgcttaac tcatgataat ttagaacaag
    10861 aaaagacgcg tctgacttcc atcctgtcct atatgagtga cggtgttatt gctacagacc
    10921 gtatcggacg gatcatcatg attaacgata tggcccagaa acagttgggg ctttctagcc
    10981 aaaagcagga gcagtatcac ctgctcgagg ttttagattt atcagaccgt tatacattga
    11041 gagatttgtt ggctcagaca cctgagattg tgattgatca caccaatgaa aacgaagaat
    11101 ttttgacttt acgtgctaat tttgccacga ttcgtagtga gagtggcctg atttctggct
    11161 tggtagtcgt cttgcatgat atgaccgagc aggccaagga agaacgggaa cgtaggctgt
    11221 ttgtgtcaaa tgtgagtcat gaattgcgta cgccattgac ttccgttaaa tcctatctag
    11281 aggctctgga tgagggggcg ctgacggagt ctgttgcacc gagttttgtt aaggtatcct
    11341 tagatgagac caaccgcatg atgcggatga ttacagacct cctaagctta tcgcgtattg
    11401 ataatcaagt tggtcagata gatgttgaac tgataaactt tactgctttt gtgaccttta
    11461 ttcttaaccg ttttgaccaa atgaaaaata ctgattctga taaggtctat acgattgttc
    11521 gtgactatca aatcagtcct atttgggttg agattgatac agataagatg actcaggttt
    11581 tagataatat cttaaataat gccattaaat attcgccaga tggcgggaca attaccttca
    11641 gcatgaagac cacagatagc cagttgattg tatccgtatc agacgaaggt ctggggattc
    11701 cgaaaacaga tttacctaga atttttgacc gattttatcg cgtagacaag gcacgatctc
    11761 gtgcccaagg cggtacaggt cttggtttag ctattgcaaa agaaatcgtg aaacaacata
    11821 agggctttat ctgggctaaa agcgaatatg gtcatggctc aacgtttaca attgtcttgc
    11881 cgtacagcaa ggacatcgca ctggatgagt gggatgattc agatgaggaa gaataggaga
    11941 atatgatcgg aaaaggtttt aattatagta ttttagcttc ggggtccagc gggaattgtt
    12001 tttacctaga aacagataaa aagaaaattt tagtggatgc tggcttatca gggaaaaagg
    12061 ttaccagtct tttggcggaa attgatcgaa agcctgagga tattgatgct atcctggtaa
    12121 cgcatgaaca tagcgatcac atccatggta tcggtgtttt agcacgtaag tatggcatgg
    12181 atatttatgc caatgaattg acctggcagg ctatggagag caaattgggc aagattgatg
    12241 tggctcaaaa acatatcttc gaattgggtg ctatgaagac ttttggtgac ctagatattg
    12301 agtcctttgg agttagccat gatgctgcat gtccgcaatt ttaccgtttt atgaaggatg
    12361 acaaatcctt tgttatgttg acggatacag gctatgtcag tgaccgcatg gttggaattg
    12421 tagaaaatgc cgatgcttat ttgattgaat cgaaccatga tattgaaatt ttgcgctcag
    12481 gttcttattc atggaatttg aagcaacgga ttctatccga taagggacat ctttgtaacg
    12541 aagatggagc tgatgccatg attcgctcgt tgggaaatcg gaccaaaaag atttacctag
    12601 ggcatttgtc aaaggaaaac aatatcaagg aattggctca catgaccatg gttaatcaat
    12661 tggctcaggc tgatttagga gttggagtgg atttccaagt ctatgacaca tcgccagata
    12721 cagcgactcc cttgaccaag atttgacaat tgatacaaac taagttatac tgaaattact
    12781 aattatacga ggtgaagtta tatgaataat taagctgctt attagtcgtt cttaaaactt
    12841 gtagtgaggt tgtattaacc gatgatacaa gggataagac tgctcataag ggcttactat
    12901 tcgtgtgact ttttccatag ggaaaggtca tgttttgcat ggcttttttc ttgttcattg
    12961 agcagaatag gggccctttt tgtgaagaaa agaggtctat atggaagtaa taaaatgtat
    13021 tcgattagag aaggaatttg cgggatgtag aggctgggca aaaagcttaa cctcgcttct
    13081 cagggttcgt gtcaac
//
LOCUS       DASFSW010000052        12846 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.052, whole genome shotgun sequence.
ACCESSION   DASFSW010000052 DASFSW010000000
VERSION     DASFSW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.052"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            25..279
                     /locus_tag="U1099_001079"
     CDS             25..279
                     /locus_tag="U1099_001079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867408.1"
                     /translation="MNKRTKKKYKPFKELWDCMEWVMFRLNRHVTRLDSLENRLENMR
                     VIDSANIQTINRKFEEYEKRVETLEREVKQLKKPWYKRRK"
     gene            279..509
                     /locus_tag="U1099_001080"
     CDS             279..509
                     /locus_tag="U1099_001080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867409.1"
                     /translation="MAEVVTMDELAKNKERGVGCRGILKAELETVTNIESIVIVSLNK
                     NNQINVSYSRNSSLEALGMLDIGKAILLDEMQ"
     gene            593..2008
                     /locus_tag="U1099_001081"
     CDS             593..2008
                     /locus_tag="U1099_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEM4867410.1"
                     /translation="MVTKTKTRLGNQRPTQSVTLHYAESLAHEAIELYQKTGRSCYPW
                     QVNLLEHIMGIDTEGLWVHQKYGYAIPRRNGKTEDVYMLELWALHHGLKILHTAHRIS
                     TSHSSFEALKKLLEDMGYVDDVDFVSNKAKGQERIEFKSTGSVIQYRTRTANGGLGEG
                     FDLLIIDEAQEYTAEQESALKYTVTDSDNPMTVMCGTPPTMVSTGTVFTNYRTKVLAG
                     KSEYSGWSEWSVEDIKDVNDTDSWYLTNPSLGYHLTERKIKAEIGDDELDHNIQRLGY
                     WPSFNQKSAISENEWKRLEVEAVPELTGKLFVGIKYGQDGANVSMAIAARTDDKKIFT
                     EVIDCQSVRNGMQWIINFLQTADIEKVVVDGANGQTLLANEMKDFKLRKPIMPTVSEI
                     ITANSVWEQAIVQETLRHANEPSLTDVVTNCDKRNIGSNGGFGYKSLYDDRDISLMDS
                     ALLAHWACYMTKPKRKQRISY"
     gene            2089..2553
                     /locus_tag="U1099_001082"
     CDS             2089..2553
                     /locus_tag="U1099_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEM4867411.1"
                     /translation="MMSFKPIETQEELDRIIGERLGRQKEKYADYDQLKNRVSELEKE
                     NGVLKSAAESTKASTSDLEKQIAELQGQVKTYEGKDLRLRVAVANGLPIELADRLAGD
                     DEEAIKADAERLASFMKPTEPTPPMASTEPNVESGSAEDEALKGMLRKMRGE"
     gene            2557..3447
                     /locus_tag="U1099_001083"
     CDS             2557..3447
                     /locus_tag="U1099_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM4867412.1"
                     /translation="MATLRSGDMFPEQTVKDIFSKVKGHSTLAKLSTQEPIPFTGTDT
                     FVFNLEGNAEIVGEGEPSNAGNATMKPKVIKPVLITYQARVSEEFVKCSEEKQLNYLK
                     SFIDGLAKKVAQAIDIASFHGLEPKSMTAASFKETNSFDGLITGNVVEYDANTIDECI
                     DAAVATVTANEGEVNGIALSPAAGAALGKIKVNGVVQYPEYRFGQNPEAFYGMKSDVN
                     KTLATVASGAKKDFAIVGDFENALKWGYAENIPLEIIEFGDPDGAGRDLKRYREVCLR
                     TEVYAGWGILDPESFARVEG"
     gene            3449..3667
                     /locus_tag="U1099_001084"
     CDS             3449..3667
                     /locus_tag="U1099_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867413.1"
                     /translation="MEYINKKTKATVETDAKLAGDWVPASEFEKAIENLTVPQLKGKL
                     DELGIEYNKKAQKAELLELLEAAKSEME"
     gene            3671..4066
                     /locus_tag="U1099_001085"
     CDS             3671..4066
                     /locus_tag="U1099_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp19/Gp15/Gp42 family protein"
                     /protein_id="HEM4867414.1"
                     /translation="MEPFATIEDLSTLWRGLKPAETKRAEALLKVVSDSLRVEAEKVG
                     KDLDKMVEAKSYFATVVKSVTIDVVARTLMTSTDQEPMTQYSESALGYSVSGSYLVPG
                     GGLFIKDSELKRLGLKKQRFGVIELYDKD"
     gene            4053..4391
                     /locus_tag="U1099_001086"
     CDS             4053..4391
                     /locus_tag="U1099_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867415.1"
                     /translation="MTRINGRPIVLIDKQVIGKDSFGHPKTADVEIEVENVLIAPATT
                     EDITNQINLTGKKVVYTLAIPKGDMHDWTNKEVRFFGQRWRTVGEPLEGLEHLIPLGW
                     NKKVQVERYG"
     gene            4384..4623
                     /locus_tag="U1099_001087"
     CDS             4384..4623
                     /locus_tag="U1099_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867416.1"
                     /translation="MARMKFKLNRAGVRDLLKSPEMQAVLTDKANAIRNRAGDGYESD
                     IYVGKTRANAMVYADSIKAKRDNKKHNTLLKAVKS"
     gene            4620..4955
                     /locus_tag="U1099_001088"
     CDS             4620..4955
                     /locus_tag="U1099_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867417.1"
                     /translation="MIEVITLNFLTEHLSVPVYTEHEEEMPDSFVVFEKTSGGKKNHL
                     NQATLAIQSYGQSLEEAAFLNEEVKQAVEKMVELPSISRVELNSDYNFTDTETKRYRY
                     QAVVDFIYF"
     gene            4971..5558
                     /locus_tag="U1099_001089"
     CDS             4971..5558
                     /locus_tag="U1099_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM4867418.1"
                     /translation="MADAKLVSSAKPDIAGAISSAPTGTSLPTNATTKLNTAFKNLGY
                     ISEDGLTNEDTRESEELKAWGGDVVDTPQTGKSDKFTYTLIEVLNVDVLKEVYGPENV
                     KGDLESGIAVEVNSKELPTHPLVVDMLLKNGAKKRIVIPNAKVLEVGEITYADSDLAG
                     YETTIQALPDSKGNTHYEYIKGASESTGTSSPSSS"
     gene            5569..5847
                     /locus_tag="U1099_001090"
     CDS             5569..5847
                     /locus_tag="U1099_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867419.1"
                     /translation="MFEVKTSTGLVLSIDQDRLENYELFEAIAAEESGDSGAMIRIVN
                     LLLGDEAKKLKDHVRTEKGLVPISALGAEIKDIFEQVKDLKNSQSSPE"
     gene            5844..6227
                     /locus_tag="U1099_001091"
     CDS             5844..6227
                     /locus_tag="U1099_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5361 domain-containing protein"
                     /protein_id="HEM4867420.1"
                     /translation="MIAVDEDALVCDLAETYGIYDYRQLPINRVAVFACGLSESSRIK
                     KVLSGQKEDLDTLLLAGIYDTVRLLFWAKTKDGQAGRNRPNSVAQALVGSKAEREERV
                     FSSDEEFERAMRALEIEIGGEEHGD"
     gene            6217..8799
                     /locus_tag="U1099_001092"
     CDS             6217..8799
                     /locus_tag="U1099_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM4867421.1"
                     /translation="MATDLGSAYVQIVPSAKGISGSISKLLGGEVDSAGRSAGSSLGA
                     SLVSALSGALAAAGIGKIIGSALGAGADLQQSFGGLDTIYDGAQESAKQFAKEAYKAG
                     ISANTYAEQAVSMGASLKQALGGDSTKAINMANVAIMDMTDNAAKMGTDIGIIQQTYQ
                     SLARGNYAMLDNLKLGFGGTKAEMERLLTTAEGLPSAMGRKFDISNYADVVEAIHLVQ
                     ESMGIAGVAAAEAQNTYSGSLAAMKASWENTLAGLSLGENIAPQLQALATTTSNFLFG
                     NFFPMVGNIFKGLPTMLGTILGDGLGRVFGGEVSGKVMGELNKLNEIILTFYDMTFGS
                     LSEKDNIDMLEKVGFSKETASKIVSLSSQIGSVITSFYDMIFGSLSEKDNIDFMAQMG
                     VDEGTATTIVNFANTIRTGFEGVWSTVQTLFGQVPGFFSSIIGAIGPIITTIMDGISK
                     LDFSGIQTLIESVLPAVQAGFQTFMSIVSPAIDSVVQSFVSMWNAAQPLITILSGALM
                     PVFQILGSFLGGVLKGVLSGVSFAFDALKVAIEFLTPVVDFLVQALNFVQPVLSTIAE
                     WIGVAIGMFGNLGTAGQGLSAFIKSAWTNIQSAIQTAGNIIRTVIDWIKLAFSGAGNA
                     VGVLKNVFSLAWMGIQDAIQVAKGVIDGVISGIKGAFTSFQSVVSSVGGAVNGVITNV
                     ISTIRGIANIDLSGAGSAIMNGFLGGLQSAWEGVKSFVGGIAGWIAENKGPISYDRVL
                     LKPAGLAIMQGLNTNLQVGFKDVMGTVSGMAGAIAKPFENQSLAYDMTSSASVDVRRN
                     LLSSTGDLAGNDGGNSLATRLANIERFLSALVDKELAVYLDGEKLAQNSYMHQGAIMA
                     REGI"
     gene            8799..9494
                     /locus_tag="U1099_001093"
     CDS             8799..9494
                     /locus_tag="U1099_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM4867422.1"
                     /translation="MNYMIINGLDTSTLADCHVLDFGKAQTSVERSEQVEVFGANGQL
                     HVSEGAYDGYNRTFIITLRHLADAMRLIEVFQSENNTVEFGYLRDSIFYCNLVSSSYV
                     PLGPHRWKVEITVSMHPFRYVKNPADVVLTSSGSVQNPGTVYSEPIIIIEGSGRVTLT
                     IGQQLMELELDTRATIDCRHKRQNIYDKNGAVKNTIRKRGPFFEIAVGRSGIATSGTV
                     SKITIKGNWRYKV"
     gene            9491..>12846
                     /locus_tag="U1099_001094"
     CDS             9491..>12846
                     /locus_tag="U1099_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867423.1"
                     /translation="MIYLKDGNIPLNLAYDDDIVQEANSTYQLSFKFPLTDGKWNLLR
                     REVFLLADDLHGEQEFFIFEVKKAKGHVQVYAKQVATLLNYYSINSISVDRVPGQTVM
                     TALAGSVKRPCPFTFFSDILDRHTFNESNVSVMAALAKDKHSIVGQWGGDLVRDKYQV
                     KLLKNGGIENESLFMYKKNLSSYEESENINNLKTRLHLKKTIKGQSEGEADRVIAVTV
                     DSPLIGQYRQIYEADIEVNDQDVTDVASLTAYGKRYFSSTLCDLVENSINLDVKGKSD
                     VSVKMFDMVSVFHERFDVDLRLKISSYHFGPMSKRLKSIGFGKVSQSFGSTVASMVAG
                     SVDKATGRLSASFEQKLQKEIDNANRHFDAEFDKRVESINDGIEQAQAEAERYADAIK
                     QEIDTEIAQVNQSMQAQSEEHDRQVADILSKTQSVESLANQAKSDAASALARANQVKT
                     EAIADARAQVATVSQALNTAKSELQTAIASADQKARDSQASATALRNDLNLQASKILE
                     QARAQTALTNRVTTVETLADGTRSTVAELSKTVNKATGDITSVTARTKTVEDTLSQTR
                     TQYEALTQTVNTQTGQIESINRKTADLQSGIDGVTERFENLRVGGTNLFKNSDFSQGE
                     KNWHKLPEIHNEATGKYVRLPARLWTMAQFVEVEQGEDYVISLYAKKISESSSSPRLN
                     IKFDSLHNEDTDYVEITKTDWKRFIFKFRAKKSGKELVYFLNRNGIEVDIKNIKMEKG
                     LLATDYSPSYEDYRSEIATYKRTAEESSAELSRQIQLADGKAVEAKTYAQQTAEGFKT
                     RLESLESYKDAEGTRANQYLTASRTETAKQLSAERAAIATNYVAKSTYDENVRGTTLK
                     LNEIKTTADTAKQNLATYQNTVDRKLEELTTSTQTLDGKINTASAKVDTVAGQIRTEI
                     SEVEGKIPITAGTRNLLKGTKELTDIYWTSNVTSDYYQGFRIARTAPSAATYIDTYRA
                     STTIVPDATEYIISFYAKSSINGTPINNHFYSPNTTTRSESNTGYIGKGTDGLAIINL
                     TTTWQRYWIKWTQTPSNTKKNVIIGRNFSANNATVEIAGVALYEGSLNKDWSPAPEDF
                     ANELSSVKTTITQTASGVEQLSTSLTTTDS"
BASE COUNT         3853 a         2414 c         3207 g         3372 t
ORIGIN      
        1 tagtaaaaca gaaaggaggt cgctatgaat aagcgtacca agaagaaata caaaccattc
       61 aaagaattat gggattgtat ggaatgggtt atgtttagat tgaatagaca tgttactcga
      121 ctggatagct tggagaatcg tttggaaaat atgagagtta ttgattccgc aaatattcag
      181 accatcaatc gtaagtttga agagtacgaa aaacgggttg aaacattaga acgtgaagtc
      241 aaacagctta agaaaccatg gtataaacgg aggaagtgat ggcagaagtg gtcacaatgg
      301 atgaattagc caaaaacaaa gaacgaggag ttggttgccg tggtattctc aaggcagagc
      361 tggagacagt taccaacatt gaatcaattg ttatcgttag cttgaacaaa aataatcaga
      421 ttaatgtttc ttatagtcgc aatagcagct tagaggcatt aggtatgttg gatattggta
      481 aagctatttt gttagacgaa atgcaatagg aaccgtgcgg aatcccatac ggttttcgat
      541 ttgccctgtc gcatggcgtt aaactaggca actacgattg aaaggatatg ctatggttac
      601 taagacgaaa acaaggcttg gcaatcagcg tccaactcaa tcggtaactt tacattatgc
      661 tgaaagtctg gcccatgagg ctatagaact ttatcagaaa acaggtcgca gctgttatcc
      721 ttggcaggtc aatttgcttg agcatatcat gggaattgat accgaaggcc tttgggtaca
      781 tcagaaatac ggctatgcta ttccacggcg aaacggtaag acagaagacg tctatatgct
      841 tgagttgtgg gctttgcacc atggattgaa aatcttgcac actgcacaca gaatcagcac
      901 gtctcattct tcttttgaag cattgaaaaa acttctggag gacatgggat acgtggatga
      961 tgttgacttt gtttctaaca aggcaaaggg gcaggaacgg attgagttta aatctactgg
     1021 gtcagtgata caataccgaa ctcggacagc caatggcggt cttggtgaag gttttgattt
     1081 gctgattata gatgaagctc aggaatacac agccgaacaa gaatcagctt tgaaatacac
     1141 ggtaacggac agtgataacc ctatgacggt tatgtgtgga acaccaccaa ccatggtatc
     1201 aacaggaact gtctttacca attaccgaac aaaggtttta gccggtaaaa gcgaatactc
     1261 gggttggtct gagtggtcag tcgaagacat caaggatgtc aacgatactg actcctggta
     1321 tttgaccaat ccgtctcttg gttatcactt aaccgagcgt aaaatcaagg ctgaaattgg
     1381 agatgacgaa cttgaccata atattcagcg tttaggttac tggccaagct ttaaccaaaa
     1441 atctgctatc tctgaaaatg agtggaagag gcttgaggtt gaggcggtgc cagagctaac
     1501 aggaaaacta ttcgttggta tcaagtatgg tcaagatggg gcaaatgtgt ctatggctat
     1561 tgcagcacga acggacgaca agaaaatctt cactgaagtg attgactgtc aatctgtccg
     1621 aaatggcatg cagtggatca tcaacttttt gcaaaccgca gacattgaga aggttgtggt
     1681 tgatggagcg aacgggcaaa cactattagc aaacgaaatg aaagatttca aacttaggaa
     1741 gccaatcatg ccgacagtca gcgaaatcat cacggccaac tctgtctggg aacaagctat
     1801 tgttcaggaa actctaagac atgctaacga gccttctttg actgatgttg tgaccaattg
     1861 tgataaacgg aatataggct cgaacggggg atttggttac aagtcccttt atgatgaccg
     1921 agacatcagc ttgatggata gtgctttgct agcgcactgg gcttgctaca tgacgaagcc
     1981 taaaagaaag caaagaatca gttattaaaa gtagcatcca gaaattgggt gcttttttaa
     2041 tgcacaaaaa ttaccgaacg cacgggaaaa tgcggagaaa ggacgttaat tatgtcattt
     2101 aaaccaattg aaacacagga agaacttgat cgcattatcg gtgaacgctt agggcgtcaa
     2161 aaagaaaaat acgctgatta tgatcagttg aaaaatcgtg tttcagaact tgaaaaagaa
     2221 aacggtgtgt tgaaatctgc agctgaatca actaaagcaa gtacatctga tttagaaaaa
     2281 caaattgcag agctgcaagg tcaagtcaaa acttacgaag gcaaagactt gcgcttgcgt
     2341 gtagcagttg ccaatggctt accgattgaa cttgctgacc gtcttgcagg agatgatgag
     2401 gaagccatca aggcagatgc cgaacgtttg gctagcttta tgaagccgac agaaccaaca
     2461 ccgccaatgg catcaacgga gccgaatgta gaaagtggct ctgcagaaga tgaagctctg
     2521 aaagggatgc ttcgtaaaat gagaggagaa taacagatgg caacattgcg atcaggggat
     2581 atgttcccag aacaaacagt aaaagatatt tttagcaaag taaaaggaca ctctacacta
     2641 gctaaacttt cgacacaaga gcctattcca ttcacaggaa cagatacatt tgtttttaac
     2701 ttggaaggca atgctgagat tgttggtgaa ggcgagccat caaatgcagg caacgctaca
     2761 atgaagccta aagtaattaa accagtttta attacttatc aggcgcgtgt atcagaagaa
     2821 tttgttaaat gctcagaaga aaaacagctt aattacctca agtcgttcat tgatgggctt
     2881 gccaagaaag ttgctcaggc aattgatatt gcctcattcc atggattgga accaaaatca
     2941 atgacggcag cttcgtttaa agaaacaaac tcatttgatg gcttgattac tggaaatgtc
     3001 gttgaatatg atgcaaacac aattgatgaa tgtattgacg cagcggtagc tacggttaca
     3061 gcgaatgagg gcgaagtcaa cggtattgca ctttccccag cggctggtgc agcacttgga
     3121 aaaatcaaag tcaatggtgt tgttcaatat cctgaatacc gttttggcca aaaccctgaa
     3181 gcattctacg gaatgaaatc agatgtcaat aaaacattag ctacagttgc tagcggagct
     3241 aagaaagatt tcgccattgt cggtgacttt gaaaatgcac ttaaatgggg atatgctgaa
     3301 aacattccgc ttgaaattat tgaatttggt gatccagatg gcgctggtcg cgacttgaaa
     3361 cgctaccgtg aagtttgctt gcgtactgaa gtatatgcag gttggggaat tctagaccca
     3421 gaatcatttg cacgtgtgga gggataacat ggaatatatc aacaaaaaaa caaaagcaac
     3481 tgttgaaaca gatgcaaaac tagctggaga ctgggtgcct gcttccgaat ttgaaaaagc
     3541 cattgaaaat ctaactgttc cacagcttaa gggaaaactt gatgaactcg gcattgagta
     3601 caacaagaaa gcccagaagg cagaactttt agaattgctg gaagctgcta aatcagaaat
     3661 ggagtagtcc atggaaccat tcgctacaat cgaagacttg tccacattgt ggcggggact
     3721 aaaaccggcc gaaaccaaga gagcagaggc tcttttaaaa gtcgtctccg actctcttag
     3781 ggttgaggct gagaaagtag gtaaggacct cgacaagatg gtcgaagcaa agtcttattt
     3841 cgccacagtg gtcaagtctg tgactattga tgttgtcgca cgtaccctta tgacatcgac
     3901 tgaccaggaa cctatgacgc agtattccga gtcagccctt ggctactctg tctctgggtc
     3961 ttatctggtc cctggtggcg gtctctttat caaagactcg gaacttaaac gcttaggtct
     4021 gaaaaagcaa agatttgggg tgattgaact ttatgacaag gattaatggt agaccgattg
     4081 tcttgattga caaacaggtc attggcaagg attcgtttgg tcatccaaag actgcagatg
     4141 tggagattga ggttgagaac gtcttgattg cgcctgcaac aaccgaggat attaccaatc
     4201 aaatcaatct tacaggtaaa aaagttgtct atactctcgc tattcctaaa ggggatatgc
     4261 acgactggac gaacaaggaa gttcgatttt ttggccaacg atggagaacg gttggcgagc
     4321 ctttggaagg gcttgagcat ttgattccgc tcgggtggaa taagaaagtg caggtggaac
     4381 ggtatggcta gaatgaaatt taagttaaac cgtgctggtg tccgtgactt gttaaagtcg
     4441 cctgagatgc aggcagtttt gactgacaag gcaaatgcta tccgaaaccg tgcaggcgat
     4501 gggtatgaat cggatatcta tgttggtaag actcgtgcta atgctatggt ttatgcggat
     4561 agtattaaag ctaagcggga caacaagaag cacaatacct tgttaaaggc ggtaaaatca
     4621 tgattgaagt tatcacgttg aactttttga ccgaacatct ttctgtgcct gtctatacgg
     4681 agcacgagga agagatgccg gatagctttg tggtctttga aaagaccagc ggtgggaaaa
     4741 agaaccatct gaaccaagcg actttagcga tacaatctta tgggcagtct ttggaagagg
     4801 ctgccttttt gaacgaagaa gtcaaacaag ctgttgagaa gatggtggaa ttgccgtcta
     4861 tcagcagggt tgagttgaac tcggactata atttcacaga tacggaaacc aaacgctatc
     4921 gctatcaagc ggtagtggat tttatttatt tttgaaaagg agaaaattga atggcagatg
     4981 caaaacttgt gtcatcggca aagcctgata ttgctggggc gatttcctca gctccaacgg
     5041 gaacgagctt gccgaccaat gcgactacta agctaaacac agcttttaaa aacttaggct
     5101 acatttcaga ggatgggttg accaacgagg acactcgtga atcggaagaa ctgaaagcct
     5161 ggggcggtga tgtggttgat actcctcaaa caggaaaatc agacaaattt acctacacac
     5221 tcattgaggt gttaaatgtg gatgtcttaa aagaggtcta tggtcctgaa aatgtgaaag
     5281 gcgaccttga atcagggatt gctgtggagg tcaactctaa ggaattgcct acgcatccgt
     5341 tggtagtgga tatgctgctg aaaaatggtg cgaagaagcg gattgtcatc ccaaatgcta
     5401 aggtgctgga agttggggag attacctacg ccgacagtga cttggctggt tatgaaacaa
     5461 ctatccaagc tctgccagat agcaaaggca atacacacta tgagtacatt aagggagcta
     5521 gcgagtcaac aggaacaagc tcgccatcgt catcgtaagg aggtttgaat gtttgaagta
     5581 aaaactagta caggtcttgt gcttagcatt gaccaagacc gtttggaaaa ctatgagctt
     5641 tttgaggcaa tcgctgctga agaatctgga gacagtggtg ccatgattcg gattgtcaat
     5701 ttattactcg gtgacgaagc gaagaaactc aaggaccatg tccgtacaga aaaagggctg
     5761 gtgccaattt ctgctcttgg tgctgaaatt aaggacattt tcgaacaagt caaagactta
     5821 aaaaactcgc aatcctcgcc agaatgattg cggtagatga ggatgctctt gtctgtgatt
     5881 tggctgaaac ctatggcata tatgattatc gacagctacc tataaatcgg gtagctgttt
     5941 ttgcttgtgg tttaagtgag tcatcacgga ttaagaaggt cttgtctggt cagaaggaag
     6001 acttggatac tctgctgctt gcaggtatct atgatacggt tcgcttactc ttttgggcta
     6061 aaactaagga cggacaggcc ggacggaatc gtccaaactc tgtcgcccaa gccttggtag
     6121 gttcgaaagc ggaacgtgaa gagagggtct tttcatctga tgaggagttt gaacgtgcta
     6181 tgcgtgcgct agaaatagag attggaggtg aggagcatgg cgactgattt gggttctgct
     6241 tatgtgcaga tagttccgtc tgctaaaggc attagcgggt cgatttcaaa attattgggt
     6301 ggcgaagttg atagcgctgg taggtcagct gggtcaagtc ttggcgcttc actcgtctct
     6361 gctttaagtg gcgcacttgc ggcagcagga attgggaaaa ttatcggttc ggctcttgga
     6421 gctggtgctg atttgcaaca atcttttggt ggtctggata ctatctatga tggtgctcaa
     6481 gagtctgcta agcagtttgc aaaagaggcc tacaaggctg gaatatcggc aaacacctac
     6541 gcagagcaag ctgtatccat gggtgccagt ctgaaacaag ctcttggtgg tgattcgacc
     6601 aaggcaatca acatggcaaa cgtggctatc atggacatga cggacaatgc ggctaaaatg
     6661 ggtaccgata tcggtatcat ccaacagacc tatcaaagtt tagcccgtgg caactatgcc
     6721 atgctagata atcttaaact cggctttggt ggcacaaagg ctgaaatgga gcgattgttg
     6781 acgactgctg agggcttgcc gtctgctatg gggcgcaagt ttgatatcag caactatgcg
     6841 gatgttgtcg aagctatcca ccttgtgcaa gaaagcatgg ggatagctgg agttgcagct
     6901 gccgaagctc agaatactta ttcagggtcc ttagctgcta tgaaagcgag ttgggaaaat
     6961 acactggcag gcttgtcctt gggcgagaat atagctccgc aattacaagc cttggcaacg
     7021 acgacttcta atttcttatt tggaaatttc tttcccatgg ttggtaatat cttcaagggt
     7081 ttgccgacaa tgcttgggac tattcttgga gacggtttag gtagagtctt tggcggagaa
     7141 gtgtctggga aggtcatggg cgagttaaat aagctaaacg aaatcatctt gacattttac
     7201 gatatgactt ttggttcctt gagcgagaaa gacaatatcg atatgctcga aaaggttggc
     7261 tttagtaaag aaacagcttc aaagattgtg tctttatcca gtcaaattgg gtcggtcatt
     7321 acgtcgtttt atgacatgat ttttgggtcg ttgagtgaga aagacaatat tgattttatg
     7381 gctcaaatgg gggtcgatga aggtactgca acaacgattg tcaattttgc aaatacaatt
     7441 cgaactggct ttgagggtgt ttggtctact gtacagacat tatttggaca agttcctggt
     7501 ttcttctctt cgattattgg agcgattgga ccaatcataa cgactatcat ggatgggatt
     7561 tcaaaactgg acttttcagg tatccaaacg ttgatagagt ctgttttgcc agcagttcaa
     7621 gcaggtttcc agaccttcat gagcattgtc agtcctgcca ttgattcggt cgttcaatcg
     7681 tttgtgtcta tgtggaatgc ggcacagcct ttgataacca ttttgagcgg tgctttgatg
     7741 cctgtttttc aaattctggg gtcattcctt ggaggagtcc tcaaaggtgt cttgtcgggt
     7801 gtgtcgtttg catttgatgc tttgaaagtt gctattgagt tcttgacacc cgtagtcgat
     7861 tttttggtac aggctttgaa tttcgttcag cctgtgctga gcacgattgc tgaatggatt
     7921 ggtgtggcta tcggtatgtt tggtaatctg ggtacagctg ggcaaggttt gagtgccttt
     7981 atcaagtcgg cttggaccaa tattcaatca gctattcaga cggctgggaa tatcatccga
     8041 acggtcattg actggatcaa gttggctttt tctggtgctg gaaatgctgt tggtgtgctt
     8101 aagaatgtct tttccttggc ttggatgggt attcaagatg ccattcaagt tgcgaaaggg
     8161 gtcattgatg gtgttatctc tggcatcaag ggtgctttta caagttttca aagcgttgta
     8221 tctagcgtcg gcggtgctgt caatggggtt atcactaatg ttatttctac cattagagga
     8281 attgctaata ttgacctttc tggggcaggt tccgccatca tgaatggctt cttgggtggt
     8341 ttgcagtctg cttgggaggg tgtcaagagt ttcgtaggcg gtattgctgg ctggattgcg
     8401 gaaaacaagg gtcctatctc atacgaccgt gtacttttga aacctgctgg tcttgctatc
     8461 atgcaaggtt taaacaccaa cttgcaagtg ggattcaagg acgtcatggg aacggtatcc
     8521 ggtatggctg gagcaattgc taagcctttt gaaaatcaat ctttggccta tgatatgacg
     8581 tcaagtgcat cggtggatgt acgtcgaaac ttgctgtctt ctactggtga tttggctgga
     8641 aatgatggcg gaaatagttt agctactcgt ctggcgaaca tcgagcgatt cctatctgct
     8701 ttggtggata aggagttggc agtttatctg gacggtgaga aattggctca gaatagttac
     8761 atgcatcaag gagcgattat ggcaagggag ggtatttaat gaactatatg attatcaatg
     8821 gcttagatac ttcgaccttg gcagattgcc atgttcttga ctttggtaaa gcacaaactt
     8881 cagtcgagcg gtctgaacaa gtcgaggtct tcggtgccaa tggtcaacta catgtcagcg
     8941 agggtgcgta tgatggctac aacaggacat ttatcatcac gctacgacat ttggcagatg
     9001 ctatgcgctt gattgaggtt tttcagtcgg aaaataatac agtagaattc ggctatctga
     9061 gggatagcat tttttactgt aatttggtat ctagtagcta cgtgccactt ggtccacatc
     9121 gttggaaggt ggaaattacg gtgtccatgc atccgtttcg gtatgttaaa aatccagctg
     9181 acgtcgtttt gacctcatca ggttctgtgc agaatccagg tacggtctat tctgaaccta
     9241 tcatcatcat agagggttcg ggacgggtga ccttgaccat tggtcagcaa ttgatggagt
     9301 tagaactaga tactcgtgcg accatcgatt gtcggcataa gcgacaaaat atctatgaca
     9361 agaatggtgc tgtaaagaac accattcgca aacgtggtcc attctttgag atagctgttg
     9421 gaagaagcgg tattgcaaca agtggaactg tctcaaaaat aacaatcaaa gggaattgga
     9481 ggtacaaggt ttgatttatc taaaagacgg taatatcccg cttaatcttg cttacgatga
     9541 tgacatcgtg caggaggcca atagcaccta ccaactttcc tttaaatttc cgctgactga
     9601 tgggaagtgg aatctgctta gacgggaagt ttttctgctg gctgatgatc tacacggtga
     9661 acaggaattt tttatttttg aagtaaaaaa agccaaaggt catgtgcagg tctatgctaa
     9721 gcaggtcgca acattattga attactactc tatcaactct atttcggttg acagggtacc
     9781 agggcagacg gttatgactg ctttggcagg tagcgttaaa cgaccatgtc catttacgtt
     9841 ttttagtgat atattagacc gtcatacgtt taatgagtcc aatgtatctg taatggctgc
     9901 tttggccaag gataaacact ctattgtcgg tcagtggggt ggtgacttgg tgcgtgacaa
     9961 gtaccaggtt aaattgttga aaaatggcgg cattgagaat gagtcgctat ttatgtataa
    10021 aaagaatctc agtagctatg aagagtctga gaacattaac aacttaaaga cacgattgca
    10081 tctcaaaaag acgatcaaag gacagtctga gggtgaggca gaccgtgtaa ttgcagtgac
    10141 tgtggatagt ccgttgattg ggcaatatcg tcaaatctac gaagcagata ttgaggtcaa
    10201 tgaccaagat gtgacagatg tggctagctt aactgcctac gggaaacgtt atttcagttc
    10261 aacactttgc gatttggttg aaaattctat caatctggat gtcaaaggca agtctgatgt
    10321 atctgttaag atgttcgaca tggtaagtgt attccacgag cgattcgatg tggatttgcg
    10381 tttgaaaatt tctagctacc atttcggacc gatgtctaag cgattgaagt caattggttt
    10441 tggcaaggtg tcgcagtcat ttggctcgac agtagcgagc atggtcgctg gcagtgttga
    10501 taaagcaact ggaagattgt cagcatcctt tgaacagaaa ctgcagaagg aaattgacaa
    10561 tgcaaaccgc catttcgacg ctgaattcga caagcgagtt gaatccatca atgacggtat
    10621 cgagcaagcc caagctgagg ctgagcggta tgctgacgct attaaacagg aaattgatac
    10681 tgaaatcgcc caagtcaacc aatccatgca agcacaatcc gaggaacacg acagacaggt
    10741 cgcagacatc ttgtccaaga ctcaatctgt cgaatcactt gccaaccagg caaagtcgga
    10801 tgctgctagt gcattggcta gagctaacca ggtaaagacc gaggctatcg cagatgcgag
    10861 agcgcaggta gcgacagtca gtcaagcctt aaacactgcc aagtccgaac tgcagacggc
    10921 tatcgctagt gctgaccaaa aggccagaga tagccaagcg agtgctacag ctttgcgaaa
    10981 cgacctcaac ttgcaagcta gcaagatttt ggagcaagca cgagcacaga cagctttgac
    11041 caatcgtgtg acgaccgttg aaaccttggc agatggtacg aggtcaacag tcgcagaact
    11101 ctctaaaacg gttaataaag cgacagggga catcactagt gttacagcta ggaccaagac
    11161 agtagaagac actctgagcc aaacgaggac ccaatacgaa gctctaacac agaccgtcaa
    11221 tactcagaca ggacagattg agagtatcaa tcgaaagact gctgacttgc agagtggcat
    11281 agatggcgtg acggagcggt ttgagaatct gagggttggt gggactaact tattcaaaaa
    11341 tagtgatttc agtcaagggg aaaaaaattg gcataaattg cctgaaatcc ataatgaagc
    11401 tacaggtaag tatgtaagat tgccagctcg tctttggaca atggcacaat ttgttgaagt
    11461 cgagcaaggt gaagattatg ttattagcct ttatgcaaag aaaatatcag aaagcagttc
    11521 tagtccgaga ttaaatataa aatttgattc gttacataac gaagatactg attatgttga
    11581 aattacaaaa actgactgga aacgatttat ttttaaattt cgtgcaaaaa aatcgggtaa
    11641 agaattagta tattttttaa atcgaaatgg tatcgaagtc gatataaaaa atataaagat
    11701 ggaaaaaggg cttttagcaa cagactactc tccatcttac gaagactatc gctctgaaat
    11761 cgcaacatac aaacgtaccg ccgaggaatc cagtgcagag ttgtcccgtc aaatccaatt
    11821 ggcagatggc aaggctgtcg aggctaagac ctacgcccag cagactgctg aaggctttaa
    11881 gactcgtctg gagagcctag aaagctacaa agatgcggaa ggtacacgag ccaaccaata
    11941 tttgactgcc agtcgaaccg aaactgccaa acagttatct gccgaacgtg ccgcgattgc
    12001 tactaactat gtggctaagt ctacctacga cgaaaatgtc agaggaacaa cgctaaagct
    12061 taacgagatt aagacgaccg ctgacactgc caagcaaaat ctagcgactt atcaaaatac
    12121 agtcgatagg aagctagaag aattgaccac aagcactcaa acacttgacg ggaaaatcaa
    12181 cactgcaagt gccaaggtcg ataccgtggc gggacagata cggactgaga ttagcgaggt
    12241 tgaggggaag atacctatca cagccggaac acgtaactta ttaaaaggca ctaaagagct
    12301 gactgatata tattggacat cgaatgtaac aagtgattac tatcaaggat ttaggatcgc
    12361 cagaacagca ccatcggcag caacatatat tgacacttat agagcgtcaa caacaattgt
    12421 tcctgatgcc acggaataca taatcagttt ttacgcaaaa tcctcgatta atggcacacc
    12481 gattaacaac cacttttata gtccaaacac gacaacaaga agcgaaagta acacagggta
    12541 catcggtaaa gggacagatg gtcttgccat tatcaatctg acaacaactt ggcaacgcta
    12601 ctggataaaa tggacgcaga caccatctaa cactaagaaa aacgtgataa tcggacgtaa
    12661 ttttagcgca aataatgcaa cagttgaaat tgctggggtg gctctatacg agggtagctt
    12721 aaataaggat tggtcaccag cgcctgaaga ttttgcgaat gaactatcat ctgttaaaac
    12781 cacaatcact caaaccgcat ccggtgtcga gcaattatcg actagcctga ctacgactga
    12841 tagcaa
//
LOCUS       DASFSW010000053        12756 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.053, whole genome shotgun sequence.
ACCESSION   DASFSW010000053 DASFSW010000000
VERSION     DASFSW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.053"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            69..767
                     /locus_tag="U1099_001095"
     CDS             69..767
                     /locus_tag="U1099_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM4867424.1"
                     /translation="MKAAYITIHDKIKEQIDNGIWKIGQRLPSERDLADEFGVSRMTL
                     RQGITLLVEEGILQRKIGSGTYVASTRVQEKMRGTTSFTELVQLQGKTPSSKLLSYTR
                     TKPNEKEVEQLGLSRGEYVIRMERVRYADNVPVVYEVASIPERLIKNVPKEDVTNHFF
                     KTLMDNGYRIGTSKQTIFARLANEKVAQYLQISKNQAILALKQVSYLEDGQAFEFVNS
                     QYVGERFEFYLENN"
     gene            870..1202
                     /locus_tag="U1099_001096"
     CDS             870..1202
                     /locus_tag="U1099_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEM4867425.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FQVSRQAVYDNIKRTEKLLEDYEMKLHMYSDYVVRSQIFDEILNKYPEDAYLKEKIAI
                     LTSIDNRE"
     gene            1215..2789
                     /gene="ffh"
                     /locus_tag="U1099_001097"
     CDS             1215..2789
                     /gene="ffh"
                     /locus_tag="U1099_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEM4867426.1"
                     /translation="MAFESLTERLQNVFKNLRRKGKISESDIQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAIGHEVIETLNPAQQIIKIVDEELTAVLGSETSEIIKSPKIPT
                     IIMMAGLQGAGKTTFTGKLANKLKQEENARPLLIAADIYRPAAIDQLKTLGQQIDVPV
                     FELGNQVPALEIVRQGLEQAKANHNDYVLIDTAGRLQIDQALMAELREIKEFAQPNEI
                     LLVVDAMIGQEAANVAREFNDQLAITGVILTKIDGDTRGGAALSVRHITGQPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASKEYDEKKSLELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMAGNPALANLKVDEREIARKRAIVSSMTPAERENPD
                     LLTPSRRRRIANGSGNSFVEVNKFIKDFNQAKTMMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMNGMDMSALEDMMGGAGMPDMSQLMGGAGMPDMSQMFGGGLKGRIGEFAMKQA
                     MKRQANKIKKAKKKRK"
     gene            2983..3651
                     /locus_tag="U1099_001098"
     CDS             2983..3651
                     /locus_tag="U1099_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEM4867427.1"
                     /translation="MNYIAIIGDIVNSKQTSNRSSIQERLKQQLNRINHSFAQDFASP
                     FTITKGDEFQALCKPNPYIFLIIDQIQLAFRDEVEIRFGIGLGEILTAIDPKLSIGAD
                     GPAYWEARKAIDFIHDNHDYGTSKIAFSSEYKQIDRVINPLLTSSDFIKAAWNRSQTD
                     LFETLISKKIYQEDFTQKPIAEKMKLSQSAFTKRLKSSGIKLYLRNRQTAMNLILQLS
                     QKEG"
     gene            3655..4395
                     /locus_tag="U1099_001099"
     CDS             3655..4395
                     /locus_tag="U1099_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEM4867428.1"
                     /translation="MSFSVISSYLIQHPVLTALLIAHFLSDFTFQSQALADDKKLHLK
                     ALFMHLFIVSLPLLILALLTPVQNGDLFFQVWLSHLAIDYVKYFLNKHHWIKDSWEAG
                     AFLADQLLHVISIIILYHTIGVNVASHSLWIEPNYLLLQILFILLISKPVNILFKLYF
                     SKYQVAEGEEEQTVTGAGALIGQLECLIMGIFLLLGQYTAIGLVFTAKSIARYDKISK
                     SQAFAEYYLIGSLFSIISVLVLYVLLIL"
     gene            4461..4982
                     /locus_tag="U1099_001100"
     CDS             4461..4982
                     /locus_tag="U1099_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4867429.1"
                     /translation="MIIWLNGPFGIGKTTLANILHKRIENSYLYDPELLGDFLQHQLP
                     KTVCPEDFQDYSVWRRSTYKIVFDLATKTDKIIIIPMTIYKKEYYQEITQQLIQDKIP
                     LEHYILLADKTTILERLDNRVNEDNIWAKRHLDVCLKAFESHIPGQRLNTDSLKPEEV
                     AEEILMLSEFTKK"
     gene            5149..6219
                     /gene="xerS"
                     /locus_tag="U1099_001101"
     CDS             5149..6219
                     /gene="xerS"
                     /locus_tag="U1099_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEM4867430.1"
                     /translation="MRRELLLEKIDQLKEIMPWFVLEYYQSKLAVPYSFTTLYEYLKE
                     YRRFFEWLQDSDLVSVERIADIPLDILEHLTKKDMEAFILYLRERPLLNANTTQNGVS
                     QTTINRTLSALSSLFKYLTEEVENEQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETMEFLDYVDKEYQVNLSKRALSSFQKNKERDLAILALLLASGVRLSEAVNLD
                     LRDVNLNMMIIEVTRKGGKRDSVNVAGFAKPYLEAYMSIRQQRYKAEKTDTAFFLSEY
                     RGLPNRIDASSIEKMVAKYSADFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAN
                     TQVTDLYTHIVNDEQKNALDQL"
     gene            complement(6289..7797)
                     /locus_tag="U1099_001102"
     CDS             complement(6289..7797)
                     /locus_tag="U1099_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEM4867431.1"
                     /translation="MSHHHVLQNGSDIRGIAIATDEYAVNLTPQATQEVVRGLIHWLT
                     QKPELAQTYQKGQLTIGIGRDSRLSGPDLVAAFTEEAVRLGVQLIDFGMATTPALFMS
                     TQYPQFKCHAGVMITASHLPHYFNGIKIFSENGGAEHEDIDFILSSSEDLPASDLGSV
                     TKADLITPYAQDLVGKIRTACGGQEKPLTGLNIIVDAGNGAGGFFAEKVLAELGADTT
                     GSQFLDPDGTFPNHVPNPDNKEAMESIRQAVLKQGADLGIIFDTDVDRAALVTKSGEI
                     LNRNNLIAVLSQIVLAEHPGTSIVTNSPTTEHLKVFIESLGGKQIRYISGYRNVINRA
                     ILANQEGVDCQLAIETSGHAAFKENYFLDDGTYVAAKILMLLPKLQAEGKSLDDLIAQ
                     LKQPLETQEVRFKLEAAGYRALGEQVIADFRNTSLEGFAFNPENEEGVRFDLTELYGD
                     GWLLLRMSLHEPLLVLQVENDQTGHIPAVLRTLSAFLDQYPAVNQEKLKALI"
     gene            complement(7873..8814)
                     /locus_tag="U1099_001103"
     CDS             complement(7873..8814)
                     /locus_tag="U1099_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM4867432.1"
                     /translation="MIAKFIREYRSFYFSYIVLATSFILLFFLYQLPTEFLQNALLLS
                     LTLLILLTVGLFWKFRNRMRALEDYVHEEALPLLNKPVDLAYLNLIEAEKEATREQLL
                     VYKSREEDLQAMVKMWSHQMKVPLAALSLMSQTDNLNQQDVNHQLLRLDNYMANLLNY
                     LKLTNHASDFRFEQVEVREVVVDLVKKYRNQFLQKDISVTIDGNWLLKSDRKWLSFAL
                     SQIIDNALKYNKTGGKVAIELNEGISISDTGIGILAEDIPRLFDEGFTGFNGREHQKA
                     TGFGLYLTKRVLNQLELAISVESQLEVGTSVTITKVQ"
     gene            complement(8811..9476)
                     /locus_tag="U1099_001104"
     CDS             complement(8811..9476)
                     /locus_tag="U1099_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4867433.1"
                     /translation="MKKEKIYIVEDDEMIVQLLKQHLGKSYQVESVQNFRAVSQEVAE
                     IKPDLVLMDISLPYYNGFYWTTEIRKTMTMPIIFISSSDDEMNAVMAMNMGGDDFVSK
                     PFSLGILDAKIGAFLRRVNQFSKSTDLSIDQFQLSLDGRFSNELEQVQLSPTETKILS
                     ALLERQGQIVSKEELLEKLWENEEFIDQNTLNVNMTRLRKKVSEVGFERIHTVRGVGY
                     LVK"
     gene            complement(9515..9772)
                     /locus_tag="U1099_001105"
     CDS             complement(9515..9772)
                     /locus_tag="U1099_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM4867434.1"
                     /translation="MSYKVILSAKAQKQIRKMDKTAASLILRYLYKHIDGCDNPRQYG
                     KALTANRSGQWRYRIGNYRVIVNIEDDKLIVTSIKMRNGSI"
     gene            complement(9762..10001)
                     /locus_tag="U1099_001106"
     CDS             complement(9762..10001)
                     /locus_tag="U1099_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM4867435.1"
                     /translation="MAAITLKVSEQDKLFMQAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADVAWAEYQADLANRIEPMTLEQMAEELGIEL"
     gene            complement(10093..12063)
                     /locus_tag="U1099_001107"
     CDS             complement(10093..12063)
                     /locus_tag="U1099_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEM4867436.1"
                     /translation="MFYLKLAGQNIRKSIGVFAPFVLASLVLFTLICSMFLLMLSPVM
                     KTMGTGAVSIGLGVVVLTIFSVIMEIYSFNFLMKQRAREFGLYNMLGMNKKKVALIAS
                     IELMMIFLLVVVLGSVLAGVFANVLYLIFVNITHYSDLHFGIEPLAFVMTAFLFAGFF
                     FLLELLAIRTIGKTSPLILFRASEKGEREPKGNVLLAALGVLSLGMGYYLSLSSKAEG
                     LAVIYRFFIAVLFVIAGTYLFYISFMTWYLKARRKNKRYFYTPEHFITTSQMIFRMKQ
                     HATGLANITLLAVMAFVTIATTTSLYTGMANMTSGLYPKETSITYKVSYRSQGVAAYQ
                     QSVLSHFPEKANDNLSYLTYQAGLVYDGGSDIVINPETIANPDFSKMAFTYFITQDDF
                     RKLGNDLPELAANQVAFMRPSEMPSLKKLVLGDQTFENVANLDQAIFPDITNTLNAAV
                     MVVSDDTVLDAVREYYESNNPQGYTVSLDYRVFTDLTDEEVASIGQQTGDSYNINDAN
                     GEFLGYAMQKSDFNNLLMGFTGSFLFTGFLLGISFLLGAALIIYYKQYSEGHEDKKSY
                     KILQEVGMSQQAVKKTINSQTLLVFFMPLAMATLHFIVALVMLKQMLMVFGVVSSSMI
                     YTVSGITLLAVVLIYFVIYKWTSRTYYRIIER"
     gene            complement(12064..>12756)
                     /locus_tag="U1099_001108"
     CDS             complement(12064..>12756)
                     /locus_tag="U1099_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867437.1"
                     /translation="TRALQDVDFKVEEGEFIAIMGESGSGKTTLLNILATLDKPTRGS
                     VILNNQDITKIKESKLADFRLRNLGFVFQDFNLLDTLSIQDNIFLPLVLARESYEEME
                     KRLKVLAPKLRIQDLLKKRPFELSGGQKQRVAIARSLITQPQIVLADEPTAALDYRNS
                     EDLLNLFEDINLDGQTILMVTHSANAASHAKRVLFIKDGRIFHQLYKAGKSNQDFAKE
                     ISLNMSALLGGE"
BASE COUNT         3826 a         2717 c         2649 g         3564 t
ORIGIN      
        1 gagtgtggcc tgaattagcc aaacgccttt atttttggta aactagaaag aagagaaagg
       61 ctagataaat gaaagcagca tatatcacaa ttcacgataa aattaaggaa caaatcgata
      121 acggaatctg gaaaattggt caacgtttac caagtgaacg cgatttagca gatgaatttg
      181 gtgtttctcg tatgacgctc cgtcaaggaa tcacactttt agtggaagaa ggtattctac
      241 aacgcaagat tggttccggt acttatgtcg ccagtacacg ggttcaagaa aagatgcgag
      301 gcacgacttc ctttactgaa ttagtacaac tccaaggaaa gacgccaagc agcaaattat
      361 tgtcctatac gcgaaccaaa cctaatgaaa aagaagttga acaattaggt ttatcccgtg
      421 gcgagtatgt tattcgaatg gagcgggtcc gctatgcaga taatgttccc gtcgtgtatg
      481 aggttgccag cattcctgaa cgcttgataa aaaatgtccc aaaagaagat gttaccaacc
      541 atttctttaa aacacttatg gacaatggtt atcgaattgg aacgagcaaa caaaccattt
      601 ttgcgcgttt agccaacgaa aaagtggctc aatatttaca aatttctaaa aaccaggcta
      661 ttctcgcact aaaacaggta tcttatctcg aggatggtca agcatttgaa tttgtaaata
      721 gtcagtatgt tggggaacgt ttcgaattct atttagaaaa caattaacat aaaaataatt
      781 atgtaaaaca tttttaaaat ccgtaatctt tctagtgatg acggattttc ttatttataa
      841 aatatggaat ttttgatata ataaagttta tggaaattga aaaaaccaac cgaatgaatg
      901 ccttatttga attctatgca gccttgctaa cagacaagca aatgaactac atcgagctct
      961 attatgccga tgattacagt ttggctgaga ttgctgaaga atttcaagtc agccgtcagg
     1021 cggtctatga taacattaaa cgaacagaaa aattgctgga agactacgag atgaagctac
     1081 atatgtattc tgactatgta gtgcgtagcc agattttcga tgaaatactg aacaagtatc
     1141 cagaagatgc ttatttgaag gaaaaaatcg ctattctaac cagtattgat aatagagaat
     1201 aagaggagaa gtatatggct tttgaaagct taacagaacg tttacagaat gtttttaaaa
     1261 atctgcgtcg taaggggaaa atttctgaga gtgatattca ggaagcaact aaggaaattc
     1321 gtctggcctt actagaagcc gacgttgcct tgccagttgt aaaagacttt atcaagaagg
     1381 tgcgtgaacg tgctatcggt catgaagtca ttgaaaccct gaaccccgct caacaaatca
     1441 tcaaaatcgt tgatgaagaa ttgacggctg tattaggttc tgaaacttca gaaattatca
     1501 aatcaccaaa aattccaact atcattatga tggctggatt gcagggggct ggtaaaacga
     1561 ccttcacagg taagttggct aataaactca agcaggaaga aaatgcccgt ccactcttga
     1621 ttgctgccga tatttatcgt ccagctgcca ttgaccagtt gaagaccctt ggtcaacaaa
     1681 ttgatgtacc tgtattcgag ttgggcaatc aagtccctgc cctggagatt gtacgccaag
     1741 gtttagaaca agccaaagct aatcataatg actacgttct gattgatacg gccggtcgtt
     1801 tgcagattga ccaagccctt atggccgaat tgagagaaat caaagagttt gctcagccaa
     1861 acgaaatcct cttggtcgtc gatgccatga ttggtcagga agctgccaac gttgcgcgtg
     1921 agttcaatga tcaactggct attactggtg tgattttgac caagattgac ggtgatacac
     1981 gtggtggtgc agccttgtct gtccgtcaca ttactggtca gccaatcaaa ttcaccggta
     2041 ctggtgaaaa aatcacagat attgaaacct tccacccaga ccgtatgtcc tcgcgtattt
     2101 tgggcatggg tgacatgctg accttgattg aaaaagccag caaagaatac gatgagaaga
     2161 aatcactaga actcgctgaa aaaatgcgtg agaatacctt tgatttcaac gatttcattg
     2221 atcagctgga ccaagttcaa aatatgggac caatggaaga cctgctgaag atgattcctg
     2281 gtatggctgg taacccagca cttgccaact tgaaagttga cgagcgtgaa attgcaagaa
     2341 aacgagctat cgtatcttct atgacaccag ccgaacgaga aaatcctgat ttgttgacac
     2401 cgagccgtcg tcgtcgtatt gctaacggtt ctggaaatag ctttgtcgaa gtcaataaat
     2461 ttatcaagga tttcaaccaa gcgaaaacca tgatgcaggg tgtcatgtca ggcgacatga
     2521 acaaaatgat gaaacaaatg ggaatcaacc caaataatct ccctaaaaac atgccaaaca
     2581 tgaacggtat ggatatgtct gctctggaag atatgatggg tggtgcaggc atgcctgata
     2641 tgagccaact gatgggagga gctggtatgc cagatatgtc ccaaatgttt ggaggcggtt
     2701 tgaaaggtag aatcggcgag tttgctatga aacaagccat gaaacgccaa gccaataaaa
     2761 tcaagaaagc caaaaagaag agaaaataat ctcttctttt ttacttttta ttcgaagaag
     2821 taagatacag caatgtaaaa tagaagtagc aaagcaggaa aaatgatttt tgaaccccaa
     2881 cattttccgt tataacggaa atattcaaaa atgtcctttt tagtggacaa tctttatatt
     2941 gtccgttata atggaaatat aataggaatc agaggacaag ttatgaatta tatcgcaata
     3001 ataggagata ttgtcaattc aaaacaaact tccaaccgct ctagtattca agagcggcta
     3061 aaacagcagc tgaatagaat caatcattcc ttcgctcagg acttcgcatc accatttacc
     3121 attacaaagg gagatgaatt tcaagcctta tgcaagccta acccttatat ttttttaata
     3181 attgaccaaa tccaactagc cttccgtgat gaggtggaga ttcgatttgg aattggtttg
     3241 ggtgagattc taacagctat cgatcccaag ctaagtattg gagcagatgg tccagcttat
     3301 tgggaagcac gcaaggctat tgacttcatt catgacaacc atgactatgg caccagcaaa
     3361 attgcatttt catctgaata caagcaaatc gatcgtgtga ttaacccctt gctaacttct
     3421 agcgatttca tcaaagctgc ctggaatcgt tcgcaaacgg atctgtttga gaccttaatc
     3481 tccaaaaaaa tctatcaaga agatttcaca caaaagccaa ttgctgagaa aatgaaactc
     3541 agtcaaagcg cctttaccaa acgtttgaaa tccagcggaa tcaaactcta cctgcgtaat
     3601 cgtcaaactg ccatgaattt gattctccag ctcagtcaga aagaaggata aagcatgtca
     3661 ttttcagtta tttctagcta tcttattcaa catccagttt taaccgcctt gcttattgcc
     3721 cactttttaa gtgattttac cttccaaagt caagcactgg cggatgataa aaaactgcat
     3781 ttgaaagctc ttttcatgca ccttttcatt gtttctctcc ctcttcttat tctagcctta
     3841 cttacccccg ttcaaaacgg agatttattt tttcaagttt ggctgagcca tcttgcgatt
     3901 gactatgtca aatattttct caacaaacac cattggataa aggactcctg ggaagctgga
     3961 gcattcctag ctgatcaatt gctacatgtc atctctatca tcattcttta tcacactatc
     4021 ggtgtcaatg ttgcttcaca ctcactctgg attgaaccaa attatctcct cctgcaaatc
     4081 ctttttatcc ttctgatcag caagcccgtc aatattcttt tcaaactcta ctttagcaaa
     4141 tatcaggtag cggaaggaga agaagagcag actgtgacag gtgcaggagc cttgattggc
     4201 caattagaat gcttgattat ggggattttt ctacttctag ggcaatacac agctatcggg
     4261 ctggtattta cagcaaaatc catcgcacgc tatgataaaa tatccaaaag ccaagccttt
     4321 gctgaatact atttaattgg ttcgctattt agtatcatta gtgtcctagt attgtacgtt
     4381 ttactgattt tataaaagaa aaaagtttac ataatttatt ttgtgtaaac ttttattata
     4441 aaaatagaaa ggaatactat atgattattt ggttaaatgg cccattcggt atcggaaaga
     4501 ctactttagc aaatatatta cacaaacgaa tagaaaactc atatctctat gatcccgagt
     4561 tactaggtga tttcttacaa catcaattac ctaagactgt atgtcctgaa gattttcaag
     4621 attattccgt ttggcgacgg agtacttata aaatagtatt tgatttagcg actaaaactg
     4681 ataagattat tattattccc atgacaatct ataagaaaga atattatcag gagattactc
     4741 agcaattgat acaggataag attcctttgg aacattatat tttgttagct gacaaaacaa
     4801 ccattttgga acgtttagat aatcgagtca atgaagacaa catctgggcc aagagacatc
     4861 ttgatgtttg tttaaaagct tttgaaagtc atattccggg gcaaaggtta aacacagaca
     4921 gtttgaagcc agaagaggtt gcggaggaaa ttctaatgct aagtgagttt accaagaaat
     4981 aatgtagact tttgggattt tgggtgtgaa aaaaaatttc cagttattta tgttttttgg
     5041 ttatccctct gatatcccca caaacatctt tccgaaaaac tataattttc ttgaaactta
     5101 tgtaagatta tgctatacta ctcatatagc taaaatcagg agaaaatgat gagacgcgag
     5161 ttattactag aaaaaattga tcaactaaaa gaaattatgc cttggtttgt tctggaatat
     5221 tatcagtcaa aattggctgt gccttacagt tttactacct tatatgaata cttgaaagaa
     5281 taccgtcgat tctttgagtg gttacaggat tcggatttgg tatctgtgga acggattgct
     5341 gacattcctt tggatattct ggaacatttg acaaaaaaag atatggaagc ctttattctt
     5401 tatctgcggg agcgtccctt gctgaacgcc aataccacgc agaatggtgt gtcgcagacc
     5461 accattaacc gtaccctgtc ggctctttct agtctcttca agtatttaac cgaagaagtg
     5521 gaaaatgagc agggcgagcc ctacttctac cgcaatgtca tgaaaaaggt atctaccaag
     5581 aagaagaagg aaaccttggc agcgcgggct gagaacatca agcaaaagct ctttttgggc
     5641 gatgaaacca tggagttctt ggactatgtg gacaaggaat accaagtcaa tctctctaaa
     5701 cgtgccctct cctctttcca gaaaaataag gagcgagatt tggcgattct ggcacttctc
     5761 ttggcttctg gcgtccgtct gtcagaagcg gtgaatttgg atcttcgaga tgtaaatctc
     5821 aatatgatga ttatcgaagt aacccgtaag ggtggtaagc gggactcggt caatgtggct
     5881 gggtttgcta agccctatct ggaagcctat atgagcatcc gtcagcaacg ctacaaggct
     5941 gaaaaaacgg atacggcctt cttcctgtcc gaataccgtg gtctacccaa tcgtatcgat
     6001 gcttcttcta ttgaaaaaat ggttgccaag tactctgcgg acttcaagat acgcgtaacc
     6061 ccccacaaac tccgtcatac cttggcaact cgcctatacg acgccacaaa gtcgcaagtt
     6121 ctggtcagtc atcagctggg ccacgccaat actcaggtca ctgacctcta cacccatata
     6181 gtcaatgatg agcaaaaaaa tgcactggat caattatagt gttacgtaat attaattatg
     6241 taaataaatt aaggagtaag cgaaaactta cccctttttc tcttaaatct aaatcaatgc
     6301 tttcaatttt tcctgattga cagctgggta ctggtccaaa aaggctgaga gagttcgtaa
     6361 aacggcaggt atgtgtccag tctggtcatt ctccacctgc aagaccaaga gtggctcgtg
     6421 gagactcata cggagcaaga gccagccgtc accataaagc tcagtcaggt caaaccgcac
     6481 tccttcttcg ttttctgggt taaaagcaaa gccttcgagg ctggtgtttc tgaaatcagc
     6541 aataacctgt tcaccaagtg ctcgatagcc tgccgcttct agtttgaacc gtacttcttg
     6601 ggtttcaagt ggttgcttga gctgggcaat caagtcgtcc aaggacttgc cttccgcttg
     6661 cagttttggt aagagcataa ggatcttagc cgctacatag gtcccgtcat cgaggaagta
     6721 attttcctta aaggcagcat gtccagatgt ttcgatagcc aactggcaat caactccctc
     6781 ttgattggct aaaatagcac ggttgatgac attacggtag cctgaaatat agcgaatttg
     6841 tttgcctccc aggctttcta taaagacctt aaggtgttca gtcgttgggg aattggtcac
     6901 aatgctggtt cctggatgtt cagctaggac aatctggctg agaacggcaa tcaggttgtt
     6961 acgattgaga atttctcctg acttggtaac cagggcggca cggtcaacgt cggtatcaaa
     7021 gataatgcct aggtctgcac cttgcttcaa gacagcctga cgaatgcttt ccatagcttc
     7081 cttgttatct gggtttggaa catggtttgg gaaggtgccg tctgggtcta ggaattgtga
     7141 accagtggta tctgctccca attctgccaa aactttctca gcaaaaaatc cgcctgcacc
     7201 gtttccagca tcgacgatga tattaagacc tgtcagtggt ttttcttgtc caccacaggc
     7261 ggtacgaatt ttgccaacta agtcttgggc ataaggcgta atcaaatctg ctttggtcac
     7321 acttccaagg tcagaagccg gcaagtcttc actactggag aggatgaagt caatatcttc
     7381 atgttctgct ccaccatttt ccgaaaaaat cttaatgccg ttgaagtaat gcggcagatg
     7441 gctggcagtg atcatgacac cagcatggca cttgaactga ggatactggg tgctcatgaa
     7501 gagagctggc gtggtagcca taccaaagtc aatcagctga acgcccagac gaacggcttc
     7561 ttctgtaaag gctgcgacca aatctggacc gctgaggcgg ctgtctcgac cgataccgat
     7621 ggtcaattgg cctttttgat aggtctgcgc cagttccggc ttctgagtta gccaatgaat
     7681 aagtccgcga actacttcct gggtggcttg cggagtgaga ttgacagcat attcgtctgt
     7741 agcgatagca attccacgaa tatctgatcc attttgcaag acgtgatgat gggacatagt
     7801 aacctcctaa ttcaaactaa atttagtata tcatattttc aaagcggttt caaaatgaaa
     7861 aaagcacagc ttctactgta cttttgtgat ggttacgctg gtcccaactt ccagttgact
     7921 ttccacggaa atagctagtt ccaactgatt caggacccgc ttggtcagat acaatccaaa
     7981 tcctgtcgct ttttggtgtt ctcggccatt aaaacctgta aatccctcgt caaacaggcg
     8041 tggaatatct tctgccagaa tcccaattcc cgtatcggaa atggaaattc cctcattcag
     8101 ttcaatggct acctttccac cagtcttatt gtacttgagg gcgttgtcga ttatctgtga
     8161 caaggcaaag ctgagccatt ttctgtcgga cttcagtagc caatttccat cgatggtcac
     8221 tgaaatgtct ttttgtaaga actgattgcg gtattttttg accaaatcca ccaccacttc
     8281 tcttacttcc acctgctcaa atcgaaaatc gctggcatgg ttggtcagct tgaggtagtt
     8341 gaggagattt gccatataat tgtccaagcg taagagctgg tgattgacat cctgctggtt
     8401 gagattgtca gtctggctca tcaaagacag ggcagccaga ggcactttca tctggtggga
     8461 ccacattttg accatggctt gcaggtcctc ttctcgtgac ttatagacca aaagctgctc
     8521 tcgtgtcgcc tccttctccg cttcaatcaa gttgagatag gcaaggtcca caggcttatt
     8581 gagcaagggc aaggcttcct catgaacata gtcttccaag gctctcatgc ggttgcgaaa
     8641 cttccagaaa agccctacag tcagtaggat taaaagggtt agggacaata gcagagcgtt
     8701 ttgcaggaat tcggtgggca gttgatagag aaagaaaagc aagataaaac tagtagccag
     8761 aacaatgtag gagaagtaaa aactccggta ttcacggata aattttgcta tcatttgacc
     8821 aagtacccca cgccacgaac cgtatgaatt cgttcaaacc cgacttcgct caccttctta
     8881 cgcagtctgg tcatgttgac attgagcgtg ttctggtcaa taaattcttc attttcccag
     8941 agtttttcca ggagttcttc cttgctgaca atctgccctt gtctttctag taaggcggac
     9001 aaaatcttgg tttccgtagg agacaattga acctgttcca attcgtttga aaatcgcccg
     9061 tctaaactaa gttggaactg gtcaattgac aaatcagttg atttgctgaa ttgattaacc
     9121 cgacgcaaga aggcgccgat tttggcatcc aaaatcccca gagaaaacgg cttagacaca
     9181 aaatcatcgc cacccatgtt catggccatg accgcattca tctcatcatc gctagaggag
     9241 ataaagataa tgggcatggt catggtttta cggatttctg tcgtccagta aaaaccgttg
     9301 taataaggca ggctaatgtc catcagcacc aagtctggtt tgatttcagc gacttcctga
     9361 ctgacagctc ggaaattctg cacactttcc acctgatacg attttcccaa atgctgcttc
     9421 aagagctgga caatcatctc atcatcttca acaatataaa tcttctcttt tttcatcttc
     9481 ttcattctgc aaaagccagg acacaggtct tggcttatat acttccattt ctcattttta
     9541 tgcttgtcac aatcaattta tcatcttcaa tattaacgat aaccctgtaa ttgccgatac
     9601 gatagcgcca ttggccagaa cgattagcag tcaaggcttt tccgtattgt ctagggttgt
     9661 cgcaaccgtc aatatgcttg tagagatagc gcaaaatcaa acttgcagcg gttttatcca
     9721 tctttcgaat ttgcttttgg gccttagcag acaaaataac cttataactc aatgcccaac
     9781 tcctcagcca tttgttctaa tgtcatcggc tcaattctat tcgccaagtc tgcttggtat
     9841 tctgcccaag caacgtctgc cacacgcgca tcgtactcgt cttcaagggc ttcaagggtt
     9901 ttagtccgaa tgagttctga caaactgaca ccttcaaact ttgccatagc ctgcataaag
     9961 agcttgtcct gctcagaaac tttcaatgta atagctgcca ttttcttacc tctttgagta
    10021 ttcctttggt attaccattg taacaccatt tccctgaaaa agcaagaaat ctcgcacaag
    10081 gcgagacttc tgctatcgtt caataatacg ataataggtt cggctggtcc acttgtaaat
    10141 gacaaagtag atgaggacaa cagcaagcaa agtaatacca ctaaccgtat aaatcatgct
    10201 ggaagaaact acaccgaaca ccatcaacat ttgcttgagc atgactagtg cgacgataaa
    10261 gtgcagggtc gccatagcca gtggcataaa gaagaccaag agtgtttgag agttgatggt
    10321 tttcttaaca gcctgctggc tcatacccac ttcctgcaaa atcttgtagg atttcttgtc
    10381 ctcatgtcct tccgagtact gtttgtagta gataatcaag gctgcaccaa gtaggaaact
    10441 aatgcccagc aagaaacctg taaagaggaa actgcctgta aagcccatca gtaaattgtt
    10501 aaaatctgat ttttgcatag catatcctag gaattctccg tttgcatcgt tgatattata
    10561 agagtcgcca gtttgttgac caatcgaagc aacctcttca tcagtcaagt cggtaaagac
    10621 acggtagtcc agactgactg tatacccttg cggattattg gactcgtaat actcacgaac
    10681 agcatccaac acagtgtcat cactgactac cataacggcg gcatttagcg tattggtgat
    10741 gtctgggaaa atagcctggt caagattggc tacattctca aaggtttgat cgcccaaaac
    10801 aagcttctta agcgacggca tttcactcgg acgcataaag gcaacttgat tggcagccag
    10861 ctctggcagg tcattcccca atttcctgaa atcatcttgg gtgatgaagt aggtaaaggc
    10921 cattttgcta aagtctggat tggcaatggt ttctggattg atgacaatat cgctcccacc
    10981 atcatagacc aaacctgctt ggtaggtcag ataggataga ttgtcattgg ctttttctgg
    11041 aaaatggctg agcaccgatt gctgataggc agctacacct tgacttctat acgaaacttt
    11101 ataggtaata gatgtttcct tgggatagag tccacttgtc atatttgcca tacctgtata
    11161 gagggatgtc gttgtcgcaa tggtcacaaa ggccatgacc gcaagtagcg taatattagc
    11221 caaacccgtt gcgtgctgct tcatccggaa aatcatctgc gaagtcgtaa tgaaatgctc
    11281 cggcgtatag aaataccgct tgtttttccg acgagccttc aaataccaag tcataaaact
    11341 gatgtagaac agataggtcc ccgcaatgac aaatagcaca gcgataaaga accgatagat
    11401 aacagctaga ccttctgctt tcgatgaaag cgataggtag tagcccatcc caagactaag
    11461 gacaccaaga gcagctagaa gaacatttcc tttcggctct ctctctcctt tttcactagc
    11521 tcggaacaaa atcagcggac tggtcttacc aatggttcga atggccagca attccaagag
    11581 gaaaaagaaa ccagcaaaga gaaaggcagt catgacaaag gccagaggct caattccaaa
    11641 atgtaaatca ctataatgtg ttatattgac aaaaatcaag tagagaacat ttgcaaacac
    11701 accagctagg acagaaccaa gaaccacaac cagtaagaat atcatcatca attcgatgct
    11761 ggctattaag gctacttttt tcttgttcat ccccagcatg ttatagagac caaattccct
    11821 cgcccgttgt ttcatcagga aattaaaact ataaatctcc ataatgactg agaaaatggt
    11881 cagaacaacg acacccagcc cgatagacac ggcacctgtt cccatggttt tcatgactgg
    11941 actaagcatg agcaagaaca tagaacaaat caaggtaaag aggaccagac tagctaaaac
    12001 gaagggagca aaaacaccta tggattttcg gatattttgt ccagcaagtt tgaggtaaaa
    12061 catctactca ccccctaaaa gtgcagacat attgagcgaa atctctttgg caaagtcttg
    12121 attgctctta cctgccttgt acaattggtg gaaaatccga ccatccttga tgaagaggac
    12181 gcgtttggca tggctggctg cgttggctga gtgagttacc atgagaattg tttgtccatc
    12241 taggttgatg tcttcaaaca aattcagcaa gtcttccgaa ttgcggtaat ctagggcagc
    12301 cgtcggttca tccgctagaa caatctgcgg ctgggtaatc aaactacggg caatggccac
    12361 gcgctgcttc tgtccaccag acagctcaaa aggacgtttt ttcagcaaat cttgaatccg
    12421 cagttttggc gccaaaactt tcaaccgttt ctccatttcc tcatagctct cacgcgccaa
    12481 taccagaggt aaaaagatat tgtcttggat agacagggtg tccaagaggt taaaatcttg
    12541 gaagacaaag cctagattac gcaaacggaa atctgccagc ttgctttcct tgattttggt
    12601 gatgtcttga ttatttaaga taacagagcc cctcgtcggc ttgtccaggg ttgctagaat
    12661 gttgagcaaa gtcgtcttac ctgaaccact ttcacccatg atggcgataa actcaccctc
    12721 ttctacctta aaatccacat cttgcaaagc acgagt
//
LOCUS       DASFSW010000054        12633 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.054, whole genome shotgun sequence.
ACCESSION   DASFSW010000054 DASFSW010000000
VERSION     DASFSW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12633
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.054"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            24..257
                     /locus_tag="U1099_001109"
     CDS             24..257
                     /locus_tag="U1099_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEM4867438.1"
                     /translation="MSKKDKKIEIQIEDSKVLVNREEFPGYRLVIGKKVIGEIAELAE
                     NNFAVIKNGNTESFYKKLEKAVGNIIENYNLSH"
     gene            309..389
                     /locus_tag="U1099_001110"
     tRNA            309..389
                     /locus_tag="U1099_001110"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Tyr,seq:gta)
     gene            665..736
                     /locus_tag="U1099_001111"
     tRNA            665..736
                     /locus_tag="U1099_001111"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:697..699,aa:Gln,seq:ttg)
     gene            860..2065
                     /gene="rpsA"
                     /locus_tag="U1099_001112"
     CDS             860..2065
                     /gene="rpsA"
                     /locus_tag="U1099_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEM4867439.1"
                     /translation="MNEFEDLLNSVSEVTPGDVVTAEVLTVDAGQANVAISGTGVEGV
                     LTLRELTNDRDADINDLVKVGETLELLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKVTRAVKGGLAVEFEGLRGFIPASMIDSRFTRNTERFVGQEFDAKIKE
                     VDPAENRFILSRRDVVEAEAAAARAEVFGKLAVGEVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSAVTVGEEVEVKVLAIDEVEGRVSLSLKATQPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDALSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAAVEGEEKAEKRAPRPRRQKREEKRDYELPETQSGFSM
                     ADLFGDIEL"
     gene            2103..2174
                     /locus_tag="U1099_001113"
     tRNA            2103..2174
                     /locus_tag="U1099_001113"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2136..2138,aa:Arg,seq:ccg)
     gene            2236..3264
                     /locus_tag="U1099_001114"
     CDS             2236..3264
                     /locus_tag="U1099_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid lyase"
                     /protein_id="HEM4867440.1"
                     /translation="MLHFENDYNKGAHPALLEALVASNQEGLSGYGTDKYTESAIEKI
                     RLATDCPHAQVTFLAGGTQTNQIVISSMLASYEGVIAADTGHIAVHEAGAIEFTGHKV
                     LALPHTDGKLVASEVENYISDFYADANHAHMVHPGMVYISHPTEYGIIYSKTELEDLS
                     KVCQQHNIPLFLDGARLGYGLAARETDLDLKTIAELTDVFYIGGTKLGALMGEALVFT
                     KNNQPKNFVSIVKHHGALLAKGRVTGVQFDCLFTDDLYLKLGRHTIAMAEQLTQILEE
                     KGFQFYLKSPTNQQFVIVKNEELTRLTEAGIAYGFWEKYDDSHSIIRFATSWSTSQAD
                     IDELINIL"
     gene            complement(3283..3666)
                     /locus_tag="U1099_001115"
     CDS             complement(3283..3666)
                     /locus_tag="U1099_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867441.1"
                     /translation="MKYVILKTPVFVWLLIFALIQIFALSSFLPEPFDTRSLLPMGVL
                     TGCSIRVSSEVKDQDGQYAPFKYLWWVILGWTVIGILFGITIKAPLELHIALIVHAIA
                     GGIGYSLIERYAVYRYHYLQTKKKG"
     gene            complement(3727..4143)
                     /locus_tag="U1099_001116"
     CDS             complement(3727..4143)
                     /locus_tag="U1099_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867442.1"
                     /translation="MNTRKNTNKKPAGLTNFQKYLTFIGSILGIITACITIYTFTAKP
                     TTSSTSPSTTISSSSATSSPSQSSSDNPLTGVQDLTSSSSETTSSTQTETSSSVESTA
                     SSSSTETSATQASETRTETSTDTEVTSGASETTSNQ"
     gene            4256..5398
                     /locus_tag="U1099_001117"
     CDS             4256..5398
                     /locus_tag="U1099_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM4867443.1"
                     /translation="MIYFDNAATTQVYPEVLKTYTEVATKIWGNPSSLHSLGSQATRI
                     LEASRKQIAELLGKDSKEIFFTSGGTEGDNWVIKGVAFEKAHLGKHIIVSAIEHPAVK
                     ESALWLKTQGFEVDLAPVNAQGFVDVSALESLIRPDTTLVSIMAVNNEIGAIQPIQEI
                     SQLLADKPTISFHVDAVQAIGKVPTERYLTDRVDFASFSGHKFHSVRGVGFVYVKAGK
                     KIAPLLTGGGQESDKRSTTENVAGIAATAKALRLTLDKAVDSQKRLAAMKQILVDELG
                     KYDDVTVFSGLEDFVPSIVTFGIKNIRGEVIVHAFEDHQIYISTTSACSSKAGKPAGT
                     LIAMGVPQKLAQTAVRISLDDDNDMGQIEQFLTIFKQIYQNTQKVR"
     gene            5400..6617
                     /gene="thiI"
                     /locus_tag="U1099_001118"
     CDS             5400..6617
                     /gene="thiI"
                     /locus_tag="U1099_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEM4867444.1"
                     /translation="MNYSEIMIRYGELSTKGKNKMRFINKLRNNIKHVLSVYPEVTVY
                     FDRDRGHVYLNGADYQEVSASLKKIFGIQNFAPSYKIEKSVPALKEAVVEIMQSIYKE
                     GMTFKIAARRSDHNFELDSRDLNQVLGDAVFTAIPNVQVQMKSPDITLRVEIRPDAAY
                     ISHEEIKGAGGLPVGTSGKGTLMLSGGIDSPVAGYLALKRGVEIEALHFASPPYTSPG
                     ALKKAHDLTRKLTAFGGNITFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRVR
                     EERGAMVIINGESLGQVASQTLESMQAINAVTNTPVIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNVEQYEARMDVEGLVERAVAGIIVTEITPKEE
                     VKDEVDSLIEDLL"
     gene            6739..8064
                     /gene="brnQ"
                     /locus_tag="U1099_001119"
     CDS             6739..8064
                     /gene="brnQ"
                     /locus_tag="U1099_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEM4867445.1"
                     /translation="MKKGALTGLLLFGMFFGAGNLIFPPALGVLSGENFWPAILGFVV
                     SGVGIAVIALIVGTLNPKGYVHEISRKISPTFATIYLVALYLAIGPFFAIPRTATTSF
                     EIGIAPLLGEANLGIWLFGFTALYFVAAYLIALNSSQILNSIGRILTPVFAILIVILV
                     VLGIFKYGSTSPLPAAEAYSAGQAFGTGFIEGYNTLDALASIAFSVVAVNTLKHLGFS
                     SKKEYVSTIWSVGLVVALAFSALYVGLAFLGNHFPVPADVLASDTNKGVYILSQATQA
                     IFGPGAQIFLAAMVIVTCFTTTAGLIVSSGEFFAERFPRFSYKVYATIFTLIGFGIAN
                     LGLNNIITFSVPVLLVLYPITICIVLIAIVNKFVPLSTYGMQLTVGVVTALSLVEVLA
                     GQFNWTAISKIISALPLAGQSLAWLLPALVGIVLSLFLPNKQESEVFEM"
     misc_feature    8132..8220
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            8256..8570
                     /gene="rplU"
                     /locus_tag="U1099_001120"
     CDS             8256..8570
                     /gene="rplU"
                     /locus_tag="U1099_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEM4867446.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLNVEAGQEVTFEEVVLVG
                     GEKTVVGTPLVAGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            8617..8910
                     /gene="rpmA"
                     /locus_tag="U1099_001121"
     CDS             8617..8910
                     /gene="rpmA"
                     /locus_tag="U1099_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEM4867447.1"
                     /translation="MLNLNLANLQFMAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTKIYPGANVGRGGDDTLYAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            9102..9251
                     /locus_tag="U1099_001122"
     CDS             9102..9251
                     /locus_tag="U1099_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867448.1"
                     /translation="MFRGSGMGSNLVKLVIDDLANRNSKAFQIAVEEEDLGTWKLYKR
                     RIVYL"
     gene            9323..10237
                     /locus_tag="U1099_001123"
     CDS             9323..10237
                     /locus_tag="U1099_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM4867449.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISIRDLEKELG
                     FQIFSRTSSGTFLTQKGMEFYEKAQALVKGFDQFEHLYLQPEEGEKTFSISSQHYDFL
                     PPLITEFSRSHPQYPHFRIFESTTVQILDEVAQGYSELGIIYLNERNTKGIMQKLDKL
                     HLQAVDLSAFQTHIYLREDHPLTKQAEVDPADLVGLPTVRFTQEKEAYLYYSENLIDT
                     SDSSVVFDVTDRATLNGILERTDAYATGSGYLDDESVNGITVIPLKGKVDNRMVYVKR
                     ANDELSSCAKDFIETMQAYFDKKKDENA"
     gene            10230..10694
                     /locus_tag="U1099_001124"
     CDS             10230..10694
                     /locus_tag="U1099_001124"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEM4867450.1"
                     /translation="MRKIGFPFLMVVLIGLDQFVKAWTVANIELDTVEPLLPGVISLA
                     YLRNYGAAWSILQNQQWFFTIMTIVAVTGLVWYYIKQIKGNIWTLFSLSLMIAGALGN
                     FIDRLRLGYVVDMFHLDFISFPVFNVADVCLTVGVGILFICIMKEESNGSKS"
     gene            10678..11571
                     /locus_tag="U1099_001125"
     CDS             10678..11571
                     /locus_tag="U1099_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM4867451.1"
                     /translation="MEVRVEVGGIRLDKALSDLTDLSRSVANEQIKAGQVLVNGQPKK
                     AKYSVQVGDVLTYQIPEVEEIDYVAEDIPLEIVFQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALLYHVKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHTKLAAELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKEAVTRFQVLERFGDYTLV
                     ELTLETGRTHQIRVHMAYIGHPVAGDEAYGPRKTLKGHGQFLHARTLGFTHPRTGEVV
                     EFTAEAPAIFQEALEKLRKAE"
     gene            11652..11990
                     /locus_tag="U1099_001126"
     CDS             11652..11990
                     /locus_tag="U1099_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEM4867452.1"
                     /translation="METLPNCPKCNSEYVYEDGALLVCPECAYEWNPADVAEEESGPV
                     AIDANGNRLADGDTVTLIKDLKVKGAPKDLKQGTRVKGIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVKKI"
     gene            12038..>12633
                     /locus_tag="U1099_001127"
     CDS             12038..>12633
                     /locus_tag="U1099_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEM4867453.1"
                     /translation="MKHQSILFYSRCLLAILAITGTALEIIKYGIGMLMYYTVQSNLL
                     VSLFAVYMVYAMSKGVDLQTSHFLRIKAAVTMSIMITCVVYHFMLAPLADDFWRLENL
                     LCHYIVPLYFLLDTLIVDRQQQYKWFDPIWWTLLPVLYMIFGLVNGLFIKIPIPDAKD
                     SPFAYFFLNVPKYGWTYVLTYAGVIFVAYLVCGFLLAGL"
BASE COUNT         3630 a         2301 c         2910 g         3792 t
ORIGIN      
        1 aatagtaaaa gtgaggtttg aaaatgagta aaaaagataa aaaaattgaa attcagattg
       61 aagatagcaa agttcttgtc aatagagaag agttccctgg ctaccgcctt gttataggta
      121 aaaaagtaat tggagaaatt gcagaattgg ctgaaaataa ctttgcagtt attaaaaacg
      181 gaaataccga aagcttttac aaaaaattag aaaaagcagt cggaaatatt attgaaaatt
      241 ataatttaag tcactaaaag gcttgtaata gtcgctattt tatgttagaa tagtgactgt
      301 tagtgcacgg agagatagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
      361 gttcgggggt tcgaatccct ctctctccat tgaacaggat acaatggacg ttaagaaatc
      421 cgtatgaaaa taggaaactg acgtagtgtg ctaaacacac aaggaagttt atctttttta
      481 ctaggatttt agtccgtgtt caactaagat acgagagatg ttctggttta ttagaaattt
      541 ctgtagaaaa ttaggagact gacgctgaat gttaacactc aaggaaggct atctattttc
      601 ctaagaaatt aatctcgagt tcaatttctt gtgattagta aataaatgaa ttgtatctat
      661 tttttggggt atcgccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg
      721 aatccagcta ccccagtaaa ggtactagat atagtctagt tcgtcaaaga tgtctaattg
      781 ttgttgtcct tgcgtgtgcc ttaaataaaa tatattttat gttgggtcga aagacctgat
      841 tgtcggaggt ttttttataa tgaacgaatt tgaagatttg ttgaacagtg taagcgaagt
      901 tacacctggt gatgtggtaa ctgcagaagt attgacagtt gatgctggtc aagcaaacgt
      961 agctatctca ggtactggtg ttgaaggcgt attgactctt cgtgagttga ctaacgaccg
     1021 tgatgctgat atcaacgacc ttgtaaaagt tggtgaaaca cttgaattgt tagtacttcg
     1081 ccaagttgtt ggtaaagata cagacactgt tacttatctt gtatctaaaa aacgtttgga
     1141 agcacgtaaa gcatgggaca agcttgttgg tcgtgaagaa gaagttgtta cagttaaagt
     1201 aactcgtgcc gttaaaggtg gtcttgcagt tgaatttgaa ggtcttcgtg gtttcatccc
     1261 agcttcaatg atcgacagcc gtttcactcg taacactgaa cgttttgtag gtcaagaatt
     1321 tgatgctaaa atcaaagaag ttgatcctgc tgagaaccgt ttcatcttgt cacgtcgtga
     1381 cgttgttgaa gcagaagctg cagctgcacg tgcggaagta ttcggtaaat tggcagttgg
     1441 tgaagttgta actggtaaag ttgcacgtat cacaagcttc ggtgcattca tcgaccttgg
     1501 tggtgtagat ggtttggtac acttgactga attgtctcac gagcgtaacg tatctcctaa
     1561 atcagctgta acagttggtg aagaagttga agttaaagtt cttgcaatcg atgaagttga
     1621 aggtcgcgta tcattgtcat tgaaagctac acagcctggt ccatgggatg gcgttgagca
     1681 aaaattggca gctggtgatg taatcgaagg taaagtaaaa cgcttgactg acttcggtgc
     1741 tttcgttgaa gtattgccag gtatcgatgg tttggtacac atttcacaaa tttcacacaa
     1801 acgtgttgaa aatccaaaag acgctttgtc agtaggtcaa gaagtaactg ttaaagttct
     1861 tgaagtgaac gcagctgatg agcgtgtatc actttctatc aaagctttgg aagagcgtcc
     1921 agcagcagtt gaaggtgaag aaaaagctga aaaacgtgct ccacgtccac gtcgtcaaaa
     1981 acgtgaagaa aaacgtgatt acgaattgcc agaaactcaa tctggattct caatggctga
     2041 ccttttcggt gacattgaat tgtaattcat ataaaaactt gccttgtgca agtttttcct
     2101 ttccaccctt agtgtaatgg atatcacgta agattccggt tcttgagatg ggggttcgat
     2161 tccctcaggg tggactaaat tgaaaagcct gcattaacag gcttttttgt tatattatat
     2221 caaggaggtt atgatatatt acattttgaa aatgactata acaaaggtgc ccatcccgcc
     2281 ttgctagaag ccttagttgc aagcaaccaa gaaggtttat ctggatatgg cactgataaa
     2341 tatactgaat ctgctatcga aaaaatcaga cttgctacgg actgtcctca tgcacaagtt
     2401 acgtttttag caggtggcac gcagaccaat caaattgtca tttcgtctat gttagcttcc
     2461 tatgaaggag tcattgcagc ggatactggt catattgcag ttcatgaagc gggggcaatt
     2521 gaatttacag gccataaggt gttggcctta ccgcatacgg atggcaaact tgtcgcaagt
     2581 gaagttgaga actatatttc cgatttctac gcagatgcta atcatgctca catggttcat
     2641 ccaggaatgg tttatatttc ccacccgact gaatatggta taatttatag caaaacagag
     2701 ctagaagatt tgtccaaagt ttgccaacag cataatattc cgctcttttt agacggtgct
     2761 cgcttgggtt atgggcttgc tgctcgtgaa acggatcttg atttgaagac aattgcagag
     2821 ctgacagatg ttttctacat tggtggtaca aaactcggtg ccttgatggg ggaagctttg
     2881 gtatttacaa agaacaacca gcctaagaat ttcgtatcga ttgtcaaaca ccatggtgcc
     2941 ttgttggcga aaggaagagt gacaggtgtc cagtttgatt gcctatttac tgatgacttg
     3001 tatcttaaat tggggcgtca tactatcgca atggcagagc aactgaccca gattttggaa
     3061 gagaagggct ttcaattcta tctcaaatcc ccgaccaatc agcaatttgt tattgttaag
     3121 aatgaagaat tgactagatt gacagaagct ggaattgcct atggcttctg ggagaaatac
     3181 gacgatagcc acagtatcat ccgctttgcg accagctggt caactagtca agcggatatt
     3241 gatgaattga taaatatatt atagcaatca aaaaagaaag ggttagcctt tctttttagt
     3301 ttgtaaataa tgatagcgat aaactgcata acgttcaata aggctgtagc ctattcctcc
     3361 tgcgattgca tgtacaatca gggcaatgtg caattctagt ggtgctttta tagtgatacc
     3421 aaatagaata ccgattacag tccatcctag tatcacccac cataagtact taaatggagc
     3481 atactgacca tcctggtctt taacttcact ggaaaccctg attgaacaac cggtcaatac
     3541 tcccatgggg agcaatgaac gagtatcaaa tggttctggt aaaaatgacg ataaggcgaa
     3601 gatttgtata agcgcaaaaa tcagtagcca aacgaaaacc ggcgttttta gaatgacgta
     3661 tttcatattg cacacctcct gaaattagta tatcaaatcc aagaaagtaa aacaaatatt
     3721 tttgtgctat tgattagaag tcgtttcgga tgccccacta gttacctctg tatcagtact
     3781 ggtttctgtc cttgtctccg aagcttgggt agcagacgtt tctgtagacg aggaagaggc
     3841 agtagactct actgaagagc tggtctcggt ttgggttgag gatgttgttt cggagcttga
     3901 acttgttaaa tcttgtactc ctgttaacgg attgtcgctt gatgattggc tgggactact
     3961 agtggcgctg gatgatgaaa tggtagtact tggagatgtt gaggatgttg taggttttgc
     4021 tgtaaaggtg tagatagtga tgcaggcagt gataatccct aaaatagacc cgataaaggt
     4081 taaatatttt tgaaagttag ttaaaccagc cggtttttta tttgtattct tacgtgtatt
     4141 catgatgtac ctccaccttt agtttaagat aggaagttta aaactatctt aaagattgcc
     4201 gaaaggcatg aaaacatgat aaaataggac tattgcaata aaaggaatat aaaaaatgat
     4261 ttattttgat aatgcggcaa cgacacaggt ctatccagaa gttctcaaga cctatacgga
     4321 agtggcgaca aaaatctggg gaaacccctc tagcctccat agtctaggca gtcaggcaac
     4381 ccgtattttg gaagcatctc gcaagcaaat cgcagaatta ctaggcaagg atagcaaaga
     4441 aatctttttc acctcaggtg gtacggaagg tgacaactgg gtcatcaagg gagtagcatt
     4501 tgaaaaggca catctgggca agcacatcat cgtgtcagct attgagcatc cagcggtaaa
     4561 agagtctgca ctttggctca aaacgcaagg ttttgaggtg gatttggctc cggtcaatgc
     4621 ccaaggtttt gttgatgttt cagccttaga aagcttgatt cgtccagata ccacactggt
     4681 ttctatcatg gccgtcaata atgaaattgg tgctattcag cccattcagg aaatctccca
     4741 gctcttagct gataagccaa ccatctcctt ccatgtggat gcagtgcagg caatcggcaa
     4801 ggtgccaacg gaacggtatt tgacagaccg agttgatttt gccagttttt caggtcacaa
     4861 attccactct gtcagaggag taggtttcgt ctatgtcaaa gctggcaaga aaatcgctcc
     4921 gctattgaca gggggaggac aggaaagcga taaacgctca acaactgaaa atgtggctgg
     4981 tattgcagca acggctaagg cgctccgctt gaccttggac aaggcggtag atagccagaa
     5041 gaggctggcg gctatgaagc agattttggt ggatgaattg ggcaaatacg acgatgtgac
     5101 agttttttct gggctagagg actttgtgcc aagcatcgta actttcggga ttaagaatat
     5161 tcgtggcgag gtcattgtcc atgcctttga agaccaccag atttacattt cgacaacctc
     5221 agcctgctcg tctaaggctg gaaaaccagc tggaacactg attgccatgg gtgttccgca
     5281 gaagttggct caaactgccg tccgtatcag cttggatgat gacaacgata tggggcaaat
     5341 cgaacaattc ctcacgattt tcaaacaaat ttatcaaaac acacagaaag taaggtagaa
     5401 tgaactattc agaaattatg attcgctatg gcgaattgtc aactaaaggg aaaaacaaga
     5461 tgcggtttat taacaaactc cgcaataata tcaagcatgt cctttctgtt tacccagaag
     5521 tgaccgttta ttttgaccgt gaccgtggtc atgtctattt gaatggggcg gattatcagg
     5581 aagtttcagc ttcattgaag aagatttttg ggatccaaaa tttcgcaccg tcttataaga
     5641 ttgaaaagtc tgttccagct ttgaaagaag cggttgtgga aataatgcag tccatttaca
     5701 aggaagggat gaccttcaag attgctgcgc gtcgtagtga ccacaatttt gaattggaca
     5761 gccgtgacct taaccaagtc cttggagatg cagtttttac agctattcca aatgtacagg
     5821 tacagatgaa gtcaccagat attaccttac gagtggaaat ccgtcctgat gctgcttata
     5881 tttcccatga agaaatcaag ggggcaggcg gtctgccagt tggtacatct ggcaagggta
     5941 cgctgatgct atcaggtggt attgattcgc ctgttgcggg ctatttagca ctgaaacgtg
     6001 gggttgaaat cgaagccctg cactttgcaa gtccgcctta tactagcccg ggtgcactta
     6061 aaaaggccca tgatttgact cgtaaattga ctgcctttgg tggtaatatc acctttatcg
     6121 aagtgccgtt tacagaaatt caggaagaaa tcaaggaaaa ggcacctgaa gcttatttga
     6181 tgaccttgac acgccgcttc atgatgcgga ttacagaccg agtacgggaa gagcgtggtg
     6241 ctatggttat tatcaatggt gaaagtctag gacaagtggc aagtcagacc ttggaatcca
     6301 tgcaggctat caatgcggtg acaaatacac ctgttatccg tccagtcgtt actatggaca
     6361 agttggaaat cattgatatt gctcaagaaa ttgatacctt tgatatttcc attcagccat
     6421 ttgaggattg ctgtaccatc tttgctcctg accgtccaaa aaccaaccct aaaatcaaaa
     6481 atgtcgagca atacgaggct cgtatggatg ttgaaggctt ggtggaacga gctgtggctg
     6541 ggattatcgt aactgaaatc acaccgaaag aagaagtgaa agacgaagtg gatagtttaa
     6601 ttgaagactt actgtagaat tcaattgttt tggcatacat ttaaatccag ctattgctgg
     6661 attttttaat acgaatcgtt cggaaaaatc gaatttgtat gatataataa gacttcagaa
     6721 aaaaacaaag gagttattat gaaaaaagga gcactaacag gattactcct gtttggtatg
     6781 ttttttggtg caggaaactt gattttccca cctgcacttg gtgtcttatc tggtgagaat
     6841 ttctggcctg ctattttagg atttgtcgtt tcgggtgtcg gtattgcagt cattgccctg
     6901 atcgtcggta ccttaaaccc aaaaggttat gtacatgaga tttctcgcaa gatttcaccg
     6961 acttttgcaa cgatttatct tgttgccttg tatttggcta tcggtccttt ctttgccatt
     7021 ccgcgtacag caacaacgtc ctttgaaatt ggtattgcgc cattgttggg cgaagcaaac
     7081 cttggaattt ggttgttcgg ctttacagct ctttactttg tggcagccta cctgattgcc
     7141 ctcaattcat ctcaaatctt aaatagtatc ggacgtattt taacccctgt ttttgcgatt
     7201 ttgattgtga ttttggttgt gctgggtatc ttcaagtatg gttcgactag tccactacca
     7261 gcagcagaag cctattcagc tggacaggca tttggtacag gatttattga aggatacaac
     7321 acccttgatg cccttgcctc aattgccttt agtgttgtag cggtgaatac cttaaaacac
     7381 cttggtttct ccagcaaaaa ggaatatgta tctaccattt ggtcagttgg tcttgttgta
     7441 gccttagctt tctcagcact atatgtcggt ttggcatttc ttggaaatca tttcccggta
     7501 cctgctgatg tcttggcgtc cgataccaat aagggtgttt atattctttc gcaagcaaca
     7561 caggctattt ttggaccagg tgcacaaatt ttcttagctg ccatggttat cgttacttgc
     7621 tttacgacaa cggctggctt gattgtttct tcaggtgaat ttttcgctga gcgtttccca
     7681 cgttttagct acaaagtata tgcgacaatt tttactttga ttggctttgg gattgccaac
     7741 ctcggtttga acaatatcat caccttctca gttccagttc ttttggttct ctatccaatt
     7801 actatctgta ttgtcttgat tgccattgtt aacaaatttg taccgctttc cacttatggt
     7861 atgcaattaa ctgtgggagt agtgacagct ttgtcattgg tagaagtgtt agcaggacaa
     7921 ttcaactgga ctgctatttc aaaaatcatc tcagctctgc cattggcagg acaatcatta
     7981 gcatggttat taccagctct tgtcggaatc gtcctctctc tattcttacc aaacaagcag
     8041 gagagcgaag ttttcgaaat gtaagaaaaa tgatacaaaa cacttgcatt tgcaggtgtt
     8101 tttgttataa taggactgtt gactatgcac acatcctgtg caaccgcacg aatgccgttc
     8161 tttcgttgag agtagagtaa gtggttcgtc ccacggtact aggcgagtct tcacatcaaa
     8221 ttcgggcaag acccaaaaat cataggaggt gcataatgag cacatatgca atcattaaaa
     8281 ctggcggcaa acaagttaaa gttgaagtcg gtcaagctat ctacgttgaa aaattgaacg
     8341 ttgaagcagg tcaagaagtt actttcgaag aagtagttct tgttggtggt gagaaaactg
     8401 ttgtgggtac tccactcgta gcaggcgcta ctgttgttgg tactgttgaa aaacaaggta
     8461 aacagaaaaa agttgttacc ttcaagtaca aacctaaaaa aggtagccac cgcaaacaag
     8521 gtcaccgtca accttacaca aaagttgtta tcaacgctat caacgcttaa tcgcgtagct
     8581 tgaagcaatc attacagaaa ttcggaggaa taacatatgt taaacttgaa tcttgctaac
     8641 ttgcaattta tggcccacaa aaaaggtgga ggttcaacgt caaacggtcg tgattcacaa
     8701 gcgaaacgcc ttggtgcgaa agctgctgac ggccaaactg tatcaggtgg ttcaatcctt
     8761 taccgccaac gtggtacaaa aatctaccca ggagctaacg taggtcgtgg tggagatgac
     8821 actctttacg ctaaagtaga aggcgttgta cgcttcgaac gtaaaggtcg cgataagaaa
     8881 caagtatctg tttacccaat cgcaaaataa gtctgaaaat ggctttacaa taggctttcc
     8941 gagttgtttt cggagagtct tttttgtaat ttgattttgc tatttttaaa agtgtacgat
     9001 agaagtgatt taagacggca atatcttctc tataggataa aaatattttg ccatctgcac
     9061 tgttgcgctg cctataagtt ctacgttcag tagtagatga aatgttcaga ggaagtggta
     9121 tgggttccaa cttagtaaaa ttagtcattg atgatttggc gaacagaaat tccaaagcct
     9181 ttcaaattgc agttgaagaa gaggatttgg gaacctggaa gttatataag agaagaatag
     9241 tctatttgtg aaaacgcaag agttcagcaa attcttgctt tttttgatat aatggtagaa
     9301 gcagttttaa gaggtatcag gtatgaatat tcaacaatta cgctacgttg tagccattgc
     9361 caacagtggt acttttcggg aagcagcaga gaaaatgtac gtttcccagc ctagtttgtc
     9421 catttccatt cgtgatttgg aaaaagagtt aggttttcaa atttttagcc gaactagttc
     9481 aggaactttt ttgacacaaa aagggatgga attctatgag aaagctcagg ctttagttaa
     9541 gggatttgat cagtttgagc atctctattt gcaacctgag gaaggtgaga agactttttc
     9601 aatttcaagt cagcattatg attttcttcc cccgttgatt acggagttct ctcgtagcca
     9661 cccgcagtac cctcattttc gcatttttga atcgactaca gtacagattt tagatgaggt
     9721 tgctcaaggt tatagcgagc ttggtattat ttatttgaac gagagaaata caaaaggaat
     9781 catgcagaaa ttggataaac ttcacttgca agctgtggat ttgtctgcat ttcagactca
     9841 tatctacctt cgtgaagatc atcctttgac aaagcaagct gaggttgatc cggcagattt
     9901 ggtaggtcta cctaccgtac ggttcactca agaaaaagag gcttatcttt attattccga
     9961 gaacttgatt gatacgtcgg attcctcggt tgtgtttgat gtgacagacc gtgcgacttt
    10021 gaatggaatt ttggagcgta cggatgccta tgcaactggt tcgggctatc ttgatgacga
    10081 gagtgtcaat ggcattaccg taattcctct caaaggaaag gtagacaatc gcatggtcta
    10141 cgttaaacga gctaatgatg aacttagttc ttgtgccaaa gactttattg aaaccatgca
    10201 ggcatatttt gataagaaga aggatgaaaa tgcgtaaaat tggatttcca tttttgatgg
    10261 tagtattaat tggtttggac cagtttgtaa aagcttggac agttgccaat attgaattag
    10321 acacggttga gccacttctt ccgggggtta taagtttggc ctatttacga aattatggtg
    10381 cagcctggtc catcttacaa aatcagcagt ggtttttcac gataatgaca attgttgctg
    10441 tgacaggcct agtgtggtac tatatcaaac agatcaaggg caatatctgg actttattca
    10501 gtctgtcttt gatgattgct ggtgctcttg ggaattttat tgatcgtctt agattgggat
    10561 atgtggtgga catgtttcac ttggatttca ttagtttccc tgtatttaat gtagcagatg
    10621 tttgtctgac agttggtgta ggtattttat ttatttgtat tatgaaagaa gagagtaatg
    10681 gaagtaagag ttgaggttgg tggtattcgt ttggataagg cattgtcgga tttgacggat
    10741 ttgtctcgtt cggttgcaaa tgagcagatt aaggctggtc aggttttggt caatggacag
    10801 cctaaaaaag ctaaatatag tgtgcaggtt ggggatgtcc taacctatca aattccagaa
    10861 gtagaagaaa tcgactatgt tgctgaggat attcctttag agattgtctt tcaggacgag
    10921 gatgttgctg ttgtaaataa accacaaggg atggtcgttc atccgtcggc tggtcacacg
    10981 tctgggacgc tagttaatgc tctcctttat cacgtaaaag atttgtcagg catcaatggt
    11041 gtcttgcgac caggtattgt ccatcggatt gacaaggaca catctggctt gctcatgatt
    11101 gctaaaaacg atgatgccca tacaaagctt gctgctgaat tgaaagataa gaaatccctt
    11161 cgtaagtatt gggccattgt acacggcaat ctaccaaacg atcgcggagt aattgaggcg
    11221 ccgattggtc gttctgaaaa agaccgcaag aaacaagcgg tgactgccaa agggaaagaa
    11281 gcggtgacac gttttcaggt tttggagcgt tttggtgact acactttggt ggaattgacc
    11341 ttggaaacag gacgaactca ccaaattcgt gtccacatgg cttatattgg tcatccggtt
    11401 gcgggtgatg aagcttacgg cccaaggaaa acactaaaag ggcatggcca atttcttcat
    11461 gccagaacgc ttggatttac ccatccgaga actggtgagg ttgttgaatt tacggcagaa
    11521 gcgccagcta tttttcagga ggcacttgaa aaactgcgca aggctgaata atagtatgaa
    11581 aaccttatca tcttgcccat tttttcaaca aatgctatac tagaacatga aaaatgttaa
    11641 ggagagtaag aatggaaact ttaccaaatt gtccaaaatg taattctgaa tatgtctatg
    11701 aagacggtgc cctcttggtt tgccctgagt gtgcatacga atggaaccca gcagatgtag
    11761 ctgaggaaga aagtggacct gttgcaattg atgctaacgg taatcgtttg gcggacggcg
    11821 atactgtgac gcttatcaaa gacttgaaag tgaaaggtgc gcctaaggac cttaaacaag
    11881 gtacacgtgt taaaggcatc cgtatcgttg aaggcgatca caatattgat tgtaaaatcg
    11941 atggttttgg tgccatgaag ttgaaatctg aatttgttaa gaaaatctaa gagagaggac
    12001 tggttaacca gtcttttcct gtttttgaaa ggatattatg aaacatcaaa gtattttgtt
    12061 ttacagtaga tgcctgttag ccattttagc catcacaggt accgccttag aaatcattaa
    12121 atatggtatc gggatgctca tgtattatac ggtacaatct aatttattag tatctctgtt
    12181 cgctgtctat atggtctatg ccatgagtaa gggtgtggat ttacagacgt cacattttct
    12241 tcggataaag gctgctgtaa ccatgtctat catgattacc tgtgtagtct atcactttat
    12301 gttggcacct ttggcagacg atttctggcg acttgaaaat ctgctctgcc attatattgt
    12361 tcccttatat tttttgttag ataccctaat agtagatcgt cagcaacagt acaagtggtt
    12421 tgacccgatt tggtggacgc ttctgcctgt tctctacatg atttttggtc tagtgaatgg
    12481 tcttttcata aaaattccaa tcccagatgc taaggacagt ccttttgctt atttctttct
    12541 caatgtccct aagtatggtt ggacctatgt tttgacttat gctggagtca tctttgtcgc
    12601 ctatctagtc tgcggttttc tattggcggg act
//
LOCUS       DASFSW010000055        12619 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.055, whole genome shotgun sequence.
ACCESSION   DASFSW010000055 DASFSW010000000
VERSION     DASFSW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.055"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..138)
                     /locus_tag="U1099_001128"
     CDS             complement(<1..138)
                     /locus_tag="U1099_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867454.1"
                     /translation="MRRLVLNIAMSLDGFIARKDGSYDWIDGHGTDKYDTVSQFDNQK
                     FF"
     gene            complement(282..1235)
                     /locus_tag="U1099_001129"
     CDS             complement(282..1235)
                     /locus_tag="U1099_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867455.1"
                     /translation="MNVSILALLISQMLIYSAPLIFTSLGGVFSERGGIVNVGLEGIM
                     VIGAFAGVVFNIEFAGTFGNATPLLAIIVGGLAGVVFAAIHAMATINFRADHVVSGTV
                     LNLLAPALGVFLVKVIYNKGQTDSITESFGKFSFPILADIPIIGEIFFKNTSLMGYVA
                     IATAFLAWFILYKTKFGLRLRSVGEHPQAADTLGINVYAMRYAGVLIAGFLGGVGGAV
                     SAQSVNINFSATTIVGSGFIALAAVIFGKWNPIGAMLASLFFGLSQSLAVVGGQLPGL
                     KDIPTVYLQIAPYLITVIALSAFFGKAVAPKADGINYIKSK"
     gene            complement(1237..2298)
                     /locus_tag="U1099_001130"
     CDS             complement(1237..2298)
                     /locus_tag="U1099_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867456.1"
                     /translation="MLNKYKNLALPLMAVVSGILLGAIVMLVFGYDPIWGYEELFYSA
                     FGNIKSIGEIFRAMAPLVFTALGFAVASRAGFFNVGLSGQAYVGWVFAGWFALANPDL
                     PRPVLILGTVLIAMLAGGIAGAIPGILRAYLGTSEVIVTIMMNYILLYSCNYIIRDVF
                     ADSLMKNTDSTVNVSANASYQTEWLRALTDNSRMNIGIFFAIIAVFVIWFLLTKTTLG
                     FEIRSVGLNPTASDYAGMSAKRTIILSMIISGALAGLGGAIQGLGTFQNVYIQSGNLD
                     IGFNGMSVALLASNSPLGIPLAAFLFGVLSVGAPGMVRAQIPPELINVVTASIIFFIG
                     VKFIFEQLLKSRNKAKGAK"
     gene            complement(2291..3826)
                     /locus_tag="U1099_001131"
     CDS             complement(2291..3826)
                     /locus_tag="U1099_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867457.1"
                     /translation="MTKDYVIEMRNITKIFGEFVANDHINLNVRRGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIVINGQSVKIDSPSKAAQLGIGMVHQHFMLVDAFTVAENI
                     ILGSETTKGGVIDLKKAIAEIKELSAKYGLEVDPTAKVADISVGAQQRVEILKTLYRG
                     ADLLIFDEPTAVLTPAEIAELLKIMKALVEEGKSIILITHKLDEIRAVADSVTVIRRG
                     KSIETVAVEGATNQDLAEWMVGRSVSFKTEKVEASPKEVILSIKDLVVNENRGIPAVK
                     GLSLDVRAGEVVGIAGIDGNGQSELIQAITGLRKVKSGSITIKGEEVVGKTPRKITEM
                     QVSHVPEDRHRDGLVLPMTVAENFALQTYYKEPISKNGILNYNIINEKARALMEEFDV
                     RGASELVPIKALSGGNQQKAIIAREIDRNPDLFIVSQPTRGLDVGAIEYIRKRLIAER
                     DKGKAVLVVSFELDEILDVSDRIAVIHDGVIHGIVEPAKTNKQELGVLMAGGHIEKGE
                     EHA"
     gene            complement(4021..5088)
                     /locus_tag="U1099_001132"
     CDS             complement(4021..5088)
                     /locus_tag="U1099_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEM4867458.1"
                     /translation="MNKKLVGLGLAAVAAVSLAACGNRASKSSSSEASSSADTSVKAA
                     IVTDIGGVDDRSFNQSAWEGLQEWGKAASLSKGNGYDYFQSASESDYITNLDSAVAGG
                     YNLVFGIGFALESAIAEVAPNNPNTHYVIVDSVVPDQDNVVSVGFADHEASYLAGVAA
                     AKSTKTNHVGFIGGMEGVIIDRFEAGFVAGAKSVNKDIKITVDYAGSFGDAAKGQTLA
                     AAQYAAGADVIFHASGGTGNGVFAAAKAENETRNEADKVWVIGVDRDQSAEGTYTSKD
                     GKSSNFVLASTLKQVGTSVKDIATKAAAGDFPGGQVLQFSLKDKGVELAETNLSEDAS
                     KAVADAKQAILDGKVEVPEKP"
     gene            complement(5169..5558)
                     /locus_tag="U1099_001133"
     CDS             complement(5169..5558)
                     /locus_tag="U1099_001133"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM4867459.1"
                     /translation="METIDLIELAVKNSQKAYVPYSKFPVSAVLVAETGEIFTGVNIE
                     NASFGLTNCAERTAIFKAISEGVKDFSEIVIYGKTEKPISPCGACRQVMAEFFDKDLK
                     VTLVAKDKSTVETTVGELLPYSFTDLT"
     gene            complement(5545..6207)
                     /gene="deoC"
                     /locus_tag="U1099_001134"
     CDS             complement(5545..6207)
                     /gene="deoC"
                     /locus_tag="U1099_001134"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEM4867460.1"
                     /translation="MKLNKYIDHTILKPETTQEQVEKILAEAKEYDFASVCVNPTWVA
                     LAAESLKDSDVKVCTVIGFPLGANTPAVKAFETKDAISNGADEIDMVINIGALKTGNY
                     DLVLEDIKAVVAASSDKLVKVIIEACLLTDDEKVKACQLSQEAGADYVKTSTGFSTGG
                     ATVADVALMRKTVGPDMGVKASGGARSYEDAIAFIEAGASRIGASSGVAIMNGAQADG
                     DY"
     gene            complement(6223..7500)
                     /locus_tag="U1099_001135"
     CDS             complement(6223..7500)
                     /locus_tag="U1099_001135"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEM4867461.1"
                     /translation="MRAVDLIQKKRDGLELSSEEIKWLIDGYVAGTVPDYQMSALAMA
                     IYFKGMSTREISDLTMSMVVTGEQIDLSAIPGIKVDKHSTGGVGDKVTLILAPLVASF
                     DIPVAKMSGRGLGHTGGTLDKLESIKGYQIEVSQADFIKQVQDTGVAVIGQSDNLVKA
                     DKLLYALRDVTATVDIIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVEEARVLA
                     QTMVDLGKAVGRKTVAVLTDMSQPVGTSIGNRLEILEAIEILQGKGREDVTEFICELA
                     QIMLGLANVEKSIEEVRQHIDNGAALKKFEEMVRAQGGDLEDLYRPVQVTQQVPVLAE
                     EDGYIVGLPALEFGLFAMKLGAGRAVKTDNLDYETGIVFHKKVGEAVSKGEQIATIYA
                     NENISENMLTNFQKNVKIDKESVKTKEIIEIIS"
     gene            complement(7497..8087)
                     /locus_tag="U1099_001136"
     CDS             complement(7497..8087)
                     /locus_tag="U1099_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM4867462.1"
                     /translation="MSNMYYEKNPTIAHDIHEIHVNLLDTPMSFMTDAGVFSKKMVDY
                     GSQVLLKTLHFEKGASVLDVGCGYGPIGLTLAKVFNTKTTLIDINSRALDLATKNAER
                     NGVIAKIYQSNIYENVDETFNHIVSNPPIRAGKSVVHEVIAGAFQRLEAHGTLSIVIQ
                     KKQGAPSAKAKMEEVFGNCQIIKKDKGYYILESVKE"
     gene            8196..9116
                     /locus_tag="U1099_001137"
     CDS             8196..9116
                     /locus_tag="U1099_001137"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEM4867463.1"
                     /translation="MKNEFLNFEQIDRATWQQLHRKTTIPLSQSELNSIKSFNDRIQL
                     HEVSDIYLPLVNLIHIYRKARKDLNFTKSLFLQKTIKPQPFIIGVSGSVAVGKSTTSR
                     LLQILIARTFKHAKVELVTTDGFLHPNAVLEERQLLNKKGFPESYDMEKLIDFLDKIK
                     NGYDCQIPVYSHEIYDIVPNKTQEIKSPDFLIVEGINVFQNPQNQRLYVSDYFDLSIY
                     VDADVEHIETWYLERFQKLLTLAKNDPNNYYHRFTQMTYPEILSIAQNTWKNINLANL
                     EKFIEPTRNRADIILHKAENHEIDKIYLKK"
     gene            9175..9423
                     /locus_tag="U1099_001138"
     CDS             9175..9423
                     /locus_tag="U1099_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEM4867464.1"
                     /translation="MEVKPLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSAIDKAETKGLIHKNKASRDKARLASKLA"
     gene            complement(9685..10914)
                     /locus_tag="U1099_001139"
     CDS             complement(9685..10914)
                     /locus_tag="U1099_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEM4867465.1"
                     /translation="MRKVSRGVLTILLSATVLSACSGGGQELSERLEAAKIEFSKEKK
                     QLEAELTALKEEVQGNFYYQQLDTDKERRVYLQFVNGLSKRMQVIDIDSVNDEIYSRV
                     YFSVAHDYPEFYWLTDEAAMAGGIDILDLEEPVYPSDLSPTQNKIEDEVNKILAQTPK
                     GSDYEKVKYFYEVIIKQTDYDLEALQTKSVTWRSQGITSVLLDKKSVCAGYSRTFQYL
                     CKKAGIDCIYVTGIAKNGQNGEFGHAWNLVKINGQYYGVDTTWGDPVFDQAISGEAHT
                     DISYDYLCVPDEILERSRIADSDLLDYWGEEQYYEPRVLVYPKCTDNSLNYYVQKGVY
                     FTSFDEAAVLQSITDQRLQGTNKVVLQFGTAEAMQQMITLASTENNAIFQALGDVGEY
                     QYYYNDQTYTFELADWF"
     gene            complement(11238..12617)
                     /locus_tag="U1099_001140"
     CDS             complement(11238..12617)
                     /locus_tag="U1099_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM4867466.1"
                     /translation="MPKRFKKLMYTDKFSFFIRYFAVFTLIFGLMTAIIFQLMRSTMY
                     QNSDNTLKRIKKEPALAVGFAIARTYEPNSVFILQDSPTGEETTSSSSDSTPVPKNQK
                     NTRAGDQLRLGANTHVLLYSKSGEMINPDTFTGLADLSLDKEKLGEIKETTVESSFGM
                     SEDYRYITIGLATDELGYYSSYDIKYATILVNVSQIKSSIESYESTVAIVMVSAWLIS
                     ILASIYLSNLSMRPILISYQKQKDFVENASHELRTPLAVLQNRLESLFRHPETTILES
                     SESIGSSLEEVRNMRLLTTNLLNLARRDDGLKVDMIDVQPNYFDEIFANYLMIAEENG
                     KTLTVNNLIHQPIRTDKVLIKQLLTILFDNAMKYTDDDGTIQMAANIKDKLVCFTVID
                     NGLGISDADKKKIFDRFYRVDKARTRQKGGFGLGLSLAQQIIKNLDGEISVRDNQPKG
                     TIFEVRLPK"
BASE COUNT         3924 a         2817 c         2123 g         3755 t
ORIGIN      
        1 aaagaatttt tgattatcaa actgtgagac cgtatcgtat ttatcggtac catgtccgtc
       61 aatccaatcg tagcttccat ccttacgtgc aataaatcca tctaaactca ttgcaatgtt
      121 taacaccaat cgtctcacaa ttagcctcat ttcatacctt agtgacaagc tattctccct
      181 attctcaacc aaaagtccct ataactcctc ttcctttgaa taggacaatt attcatttcc
      241 tgtacagcaa agaacatgat aaaaaggtcc actggacctt tttatttaga tttgatatag
      301 ttgataccgt ctgctttagg agcgactgct ttaccaaaga aagcagacag agcaataaca
      361 gtaatcaaat atggtgcaat ttgaagataa accgttggaa tatctttcaa tcctggtaat
      421 tgtccaccca ctactgccaa actttgcgaa agaccgaaga atagactggc aagcatagcg
      481 ccaattggat tccacttacc aaaaattaca gctgctagag cgatgaaacc tgaacctacg
      541 attgttgttg cagagaagtt gatattcact gattgagcac ttacagctcc accaacacca
      601 ccaaggaaac cagcaatcaa cacacctgca taacgcatag cgtagacatt gattcccaaa
      661 gtatccgctg cttgcggatg ttcacctact gaacgaaggc gcaaaccgaa cttggtttta
      721 taaaggatga accaagcaag aaatgctgtt gcaatagcta cataacccat caagcttgta
      781 tttttgaaga aaatttcacc aatgattgga atatctgcca agatagggaa tgaaaattta
      841 ccaaatgatt cagtaatact atcagtttga cccttattat agataacctt gactaggaaa
      901 actcccaatg ctggagccaa aaggttcaac accgtacctg atacaacgtg gtctgcacgg
      961 aaattaatcg tcgccatcgc atgaatagct gcaaagacaa caccagcaag acctcctaca
     1021 ataatagcta ataatggagt agcattacca aatgtccctg caaattcgat attgaaaaca
     1081 acaccagcaa aggcaccgat aaccatgatt ccttctagac caacgtttac aataccaccg
     1141 cgttcagaga aaactcctcc aagactggta aagattaagg gtgctgaata aatcaacatt
     1201 tgcgaaatga gtaaggcaag aattgaaaca ttcatcttat ttcgctcctt tcgctttatt
     1261 tctagatttt aataattgtt caaaaataaa tttcacaccg ataaagaaaa tgattgatgc
     1321 cgtaacaacg ttgatcaatt caggtggaat ctgtgcacgt accatacctg gggcaccgac
     1381 agaaagaaca ccgaatagaa aggcagccaa tggaatacct aacggtgaat tagaagctaa
     1441 aagcgctact gacataccat taaaaccaat atccaagtta ccgctttgga tataaacgtt
     1501 ttggaaagta cccaaacctt ggatagctcc cccaagaccc gcaagagcac ctgaaataat
     1561 catagataga ataatcgtac gtttcgcaga catacccgca taatccgatg ctgttgggtt
     1621 aagccccact gagcgaatct caaaaccaag agtcgttttg gtcaatagga accaaatcac
     1681 aaaaactgca ataatggcaa agaaaatacc aatattcata cgtgagttat ctgtcaatgc
     1741 acggagccac tctgtttggt aagaggcatt tgctgacacg ttaactgtcg agtctgtatt
     1801 cttcatcaaa ctatcagcaa atacatcacg aatgatatag ttacatgaat agagtaagat
     1861 gtagttcatc ataatcgtaa cgataacttc acttgttccc aaataggcac gtagaatacc
     1921 tggaatagct ccagcaatac cgcctgccaa catagcaatc aagactgttc ccaaaatcaa
     1981 aactggacgt ggtaagtcag gattcgccaa ggcaaaccat ccagcaaaaa cccaaccaac
     2041 ataggcctga ccagacaaac caacgttgaa gaaacctgca cgactagcta ctgcgaaacc
     2101 gagagcagtg aaaacaagtg gtgccattgc acggaaaatt tcaccaattg atttgatatt
     2161 accaaaagct gaatagaata actcttcata gccccaaatt ggatcgtagc caaaaacaag
     2221 catgacaatc gctccaagta ggataccact gaccacagcc attaatggaa gggccaaatt
     2281 cttatattta ttaagcatgt tcttctccct tctcaatatg tccacctgcc atcaagacac
     2341 ctaactcttg tttattggtc ttagcaggtt caacaatacc atggatgaca ccatcatgga
     2401 taacagcaat tctatctgat acatccaaaa tttcatccaa ttcgaagctg acaacaagaa
     2461 cagccttacc cttatcacgt tctgcaatga gacgcttacg aatatactca atagcaccaa
     2521 catccaaacc gcgtgttggt tggctaacaa tgaaaaggtc tgggttgcga tcgatttcac
     2581 gcgcaataat agctttttgc tggttaccgc ctgaaagagc cttgattgga acgagttcac
     2641 tcgccccacg aacgtcaaat tcttccatta acgcgcgtgc tttttcatta atgatattat
     2701 agttcaagat accatttttt gaaatcggtt ctttatagta agtttgaagt gcaaagttct
     2761 cagcaacagt catcggaaga acgagtccat cacgatgacg atcttcggga acgtggctca
     2821 cttgcatttc tgtaatttta cgtggagttt taccaactac ttcctcacct ttgatagtga
     2881 ttgagccaga ttttacctta cgtaaaccag tgattgcctg aatcaactca ctttgaccgt
     2941 ttccgtcgat accggcaata ccaacaacct cacctgctcg aacatcaagc gacaagccct
     3001 taactgctgg aataccacgg ttttcattga caaccaaatc cttaattgac aaaatgactt
     3061 ctttaggact cgcttcaact ttctctgttt taaaggaaac ggaacgtcca accatccact
     3121 cagccaaatc ttggtttgtt gctccttcaa cggcaactgt ttcgattgat ttacctcgac
     3181 gaataactgt tacactatcg gcaactgcac gaatttcatc caatttgtgc gtaattaaaa
     3241 taatagattt accttcttca accaaggctt tcataatttt caagagctct gcaatttccg
     3301 caggggtcaa tacagctgtc ggctcatcaa aaattaaaag gtcggctcca cggtaaagtg
     3361 ttttaaggat ttctacacgt tgctgtgcac ccacagaaat atctgcaacc tttgcagttg
     3421 gatcaacttc aagaccatat ttagcagaaa gttctttaat ttctgcgata gcttttttaa
     3481 ggtcaatcac gccacctttg gtggtttcag aaccaagaat gatgttctca gcaacagtga
     3541 aagcatcaac caacataaag tgttggtgca ccataccgat acccaattgg gctgctttgg
     3601 atggagagtc aatcttcacg gattgaccgt tgataacaat ttcaccgctt gtaggttcca
     3661 acaagccagc aagcatgttc atcagagtag atttacccgc accattctca ccaagaaggg
     3721 catggatttc accacgtctg acattcaaat tgatgtggtc atttgctaca aattcaccaa
     3781 atatcttagt gatattgcgc atttcaatga cataatcctt agtcataatc tattatcctt
     3841 tctgactata gccttctgaa aattcttcag tactactttg agtacttaaa aactcttcat
     3901 caactatacc atttttgtca gtaaaaccta ccatagctga taggctttac tgagaaaaat
     3961 ctcagcccga aaacggcgcc ccgtcgggtc gccgaatcgg aattgtcgaa aagaaattca
     4021 ttaaggtttt tcaggaactt ctactttgcc atccaaaatt gcttgtttag catctgcaac
     4081 tgctttagaa gcatcttctg aaagattagt ttctgccaat tcaacacctt tatctttcaa
     4141 tgagaattga agtacttgac cgccagggaa atcacctgca gctgccttag ttgcaatatc
     4201 tttaaccgaa gtaccaactt gtttcaatgt tgaagccaat acaaagttag aagacttacc
     4261 atctttagaa gtgtatgtac cttctgctga ttggtcacgg tcaacaccga ttacccaaac
     4321 tttatctgct tcattacgag tttcgttttc tgctttagca gctgcaaata caccgttacc
     4381 agtaccacca gaagcgtgga aaataacatc tgcacctgca gcatattgag cagctgcaag
     4441 agtttgacct ttagcagcat caccaaatga acctgcatag tcaactgtaa tcttgatgtc
     4501 tttgtttaca gacttagcac cagcaacgaa accagcttca aagcgatcga tgataacacc
     4561 ttccatacca ccgatgaaac ctacgtgatt tgttttagtt gatttagctg cagcaacacc
     4621 agcaagatat gatgcttcat gatctgcgaa acctacgcta actacattgt cttgatctgg
     4681 aacaacacta tccacgatta catagtgtgt attaggatta tttggtgcaa cctcagcaat
     4741 cgcactctcc aaagcaaaac caataccgaa cacaagattg taaccaccag caacagctga
     4801 atcaaggttt gtgatataat ctgattcact agctgattgg aagtaatcgt agccattacc
     4861 tttggaaaga ctagctgctt taccccactc ttgaagacct tcccaagcag attggttaaa
     4921 tgaacggtca tcaacaccac caatgtcagt aacgatagca gctttcacag atgtgtccgc
     4981 tgatgaagat gcttctgagc tactgctttt tgaagcacgg ttaccacatg cagccaaaga
     5041 tacagccgca acagctgcta gaccaagtcc aacaagtttc ttgttcattt ctgaaccctc
     5101 ctaaaaatct ttttgcaact aaattgttgc agagtaatat tgctacagtc aaatgactgt
     5161 catacagact atgttaagtc tgtaaaggaa tatggtagta attccccgac tgtcgtctcg
     5221 actgtcgatt tatctttagc gaccaaggtc acttttaaat ccttatcaaa aaattctgcc
     5281 ataacttggc gacaagctcc acaaggagat attggttttt ctgttttacc ataaattacg
     5341 atttcagaaa aatctttcac accttcagaa atagccttga aaatagctgt cctttcagcg
     5401 caattggtca aaccaaaact tgcattttca atgtttacac ctgtaaatat ttctccagtc
     5461 tcagctacaa gaactgcact aactggaaac tttgagtacg gtacgtatgc tttttgactg
     5521 ttttttacag ctaattcaat taaatcaata gtctccatcc gcctgtgctc cattcatgat
     5581 tgccacgcct gatgacgcac caatacgaga cgctcctgct tcgataaagg caatggcatc
     5641 ttcatacgaa cgtgcaccac ctgaagcctt cactcccatg tcaggaccca ctgtttttct
     5701 cattaaggca acatccgcta ctgtggcacc gcctgtagaa aatcctgtag atgttttaac
     5761 atagtccgca ccagcttctt gagataattg acaagctttc actttctcat catcagtaag
     5821 aaggcatgct tcgatgataa ctttgactag tttatcacta cttgctgcta caacagcttt
     5881 aatatcctcc aaaaccaaat cgtaatttcc agttttcaga gcaccgatat tgataaccat
     5941 gtcaatctca tctgctccat ttgaaattgc atcttttgtt tcgaacgctt ttacagcagg
     6001 tgtgtttgct ccgagaggga aaccaataac cgtacaaact tttacatcac tatctttcaa
     6061 gctttcagct gctaaagcaa cccaagttgg gttaacacag acacttgcaa aatcatattc
     6121 ttttgcttct gctaaaattt tttcaacctg ctcttgtgtt gtctcaggtt tcaagattgt
     6181 gtggtcaata tatttattca atttcatagc tttctccgta cattaagaaa taatctcgat
     6241 gatttctttc gtttttacac tttctttatc tattttaaca tttttttgga aatttgtaag
     6301 catgttttcc gaaatatttt cattggcata aatcgttgcg atttgttcgc ctttagaaac
     6361 agcttccccc actttcttat ggaaaacaat acctgtttca taatctaaat tgtcagtctt
     6421 gactgcacga cctgcaccaa gtttcattgc aaagagacca aattccaaag caggaagtcc
     6481 cacaatatag ccatcctctt ccgcaagaac tggaacttgc tgtgtaacct gaactggacg
     6541 atataaatct tctaagtcac caccttgagc acggaccatc tcttcaaatt tcttcaatgc
     6601 tgcaccattg tcaatatgtt gacgtacttc ttcaatgctt ttctcaacat tagcaagccc
     6661 caacataatt tgggctagtt cacagataaa ttcggtaaca tcttctcgac ctttaccctg
     6721 taaaatttca atagcctcta aaatttccaa acgatttcca atcgaagttc caactggttg
     6781 agacatgtca gttaaaacag caactgtctt acgaccaacc gctttcccaa gatccaccat
     6841 agtttgagct agaacccgtg cttcttcaac agtcttcatg aaggcacctt ctcccaccgt
     6901 gacatctaga agaatcgcat ccgcacctgc ggcaattttc ttagacatta ctgagctggc
     6961 aatcagcgga ataatgtcaa cggttgctgt cacgtcacga agggcataga gtagtttatc
     7021 agctttgaca agattatctg actgaccaat aactgcaaca ccagtatctt gaacttgctt
     7081 gatgaaatct gcttgactaa cttcaatctg gtatcctttg attgactcta acttatccaa
     7141 ggttccacca gtatgaccaa gcccacgacc gctcatcttt gcaactggaa tatcaaaact
     7201 tgctaccaat ggcgctaaaa tgagtgtaac cttatctccg acaccacctg tagaatgctt
     7261 atctaccttg atgcctggaa ttgcagacaa atcaatctgt tcacccgtta caaccatgga
     7321 catcgtcaaa tcagaaatct cacgcgttga catcccttta aaataaatcg ccatcgctaa
     7381 ggctgacatt tgataatccg gtactgtccc agctacatat ccatcaatca accatttgat
     7441 ttcttcagac gatagttcta agccatcacg ctttttttga attaaatcaa ctgctctcat
     7501 tctttcacac tttcaaggat gtagtaaccc ttatctttct ttataatctg gcagttgccg
     7561 aatacctctt ccatcttagc tttagcactc ggtgcgcctt gtttcttttg aatgacaatg
     7621 gacaaggttc cgtgtgcctc taagcgttga aaggccccag caattacttc atgtacaacc
     7681 gattttccgg cccgaatagg tggatttgaa acaatatgat tgaatgtttc gtccacgttt
     7741 tcgtagatat tggattgata aattttagcg ataacaccat tcctctctgc attttttgta
     7801 gctaaatcca aagctctgct attaatatca atcagtgttg tcttggtgtt aaacacctta
     7861 gcaagtgtca aaccaattgg tccataacca caaccaacat ccaacaccga tgcccctttt
     7921 tcaaaatgaa gggtttttaa aagcacttga ctaccataat caaccatctt cttgctaaaa
     7981 acacctgcat ctgtcatgaa agacattggg gtgtctagta agtttacgtg gatttcatga
     8041 atatcatgtg ctattgtagg atttttttcg taatacatat tggacatgta cttattataa
     8101 catttcctaa aaacgttttc aagagttttt cttacatgaa aaatatgata aaatgaaatc
     8161 gatattgaaa cgcttaccta taggaagaat tatgtatgaa aaatgaattt ttaaattttg
     8221 aacaaattga ccgagccact tggcaacaac tacatcgtaa gactaccatt ccacttagtc
     8281 aaagcgaact aaattctatt aaaagtttta acgaccgcat ccaattacat gaggtttctg
     8341 acatctatct tcctttggtt aatcttatcc atatctatcg gaaagcacgc aaagacctaa
     8401 attttacgaa gagtttattt ctacagaaga ctatcaagcc acaaccattt atcattggcg
     8461 tttctggaag tgttgctgtc ggcaaatcaa caactagtag attgctgcaa attcttatcg
     8521 ctcggacatt taaacacgcc aaggttgaat tagtaacaac agatggtttt ctccatccaa
     8581 atgcggtctt agaagaacgg caactgttaa ataaaaaagg ttttcctgaa tcctatgaca
     8641 tggagaaatt gattgatttc cttgataaga taaaaaacgg ctacgactgc caaattcctg
     8701 tctattcaca tgaaatctat gatattgtcc ccaacaaaac tcaggaaatt aaatctccag
     8761 atttcttgat tgttgaagga atcaatgtat ttcaaaatcc acaaaaccag cgcctctatg
     8821 tcagtgacta ttttgaccta tctatatacg ttgacgctga tgtggagcat attgaaacct
     8881 ggtacttgga gcgattccaa aaattattga cattggcaaa aaatgaccct aacaactatt
     8941 accatcgttt tacccagatg acgtaccctg agattctatc tattgcccag aatacttgga
     9001 aaaatattaa tctagctaac ttagaaaaat tcattgaacc aaccagaaac cgtgctgata
     9061 ttattcttca taaggccgaa aaccatgaga ttgataaaat ttatctaaaa aaataaattt
     9121 cacttgtcaa aaatactctt ttcagatata atggtatagt tagtacaaga aagggtggag
     9181 gtgaaaccat tggcaaacat taagtcagct atcaaacgcg ctgaattgaa cgttaaacaa
     9241 aacgagaaaa actcagcaca aaaatcagct atgcgtactg caatcaaagc atttgaagct
     9301 aacccatctg aagagctttt ccgtgctgct agttcagcaa tcgataaagc agaaactaaa
     9361 ggtttgattc acaaaaacaa agcaagccgc gataaagcac gtcttgcatc aaaacttgct
     9421 taattaaaaa agaaaccttt gggtttcctt tttttatttt ttccatcata aggaggcggg
     9481 acaaagtgga tcagttttcc cgcgatgact aacatcatta actcgtcaac cacaaagccc
     9541 tcactatgat tatacaccta gtgagggctt tgtggttgtt ttcattattc tgtgggtgag
     9601 ggcggagcca ccatggatgt ctttcccgct ccctattttt tattattagg gaagaacacg
     9661 ctcctagaga ttcaaactaa ttccttaaaa ccaatctgcc agttcaaatg tataggtttg
     9721 gtcattataa tagtactgat actctcctac atcgcccaaa gcctgaaaaa tagcattatt
     9781 ttcagtagag gccaatgtaa tcatttgttg cattgcttct gctgttccga attgcaagac
     9841 tactttattc gtaccttgta gccgctggtc agtgatggac tgtaaaactg cagcttcatc
     9901 aaagcttgtg aaataaactc ccttttgaac atagtaattc aatgaattat ctgtacactt
     9961 tggatagacc aatactctag gttcgtaata ttgttcttct ccccagtagt cgagtaggtc
    10021 gctatcagca attctacttc tctctaatat ttcatcaggg acacatagat aatcgtatga
    10081 aatatctgta tgcgcttctc cacttatagc ttgatcaaaa actggatctc cccaagtcgt
    10141 atcaacacca tagtattgtc catttatctt gactagattc caagcatgcc caaactcacc
    10201 gttttgccca ttttttgcaa tccctgtgac atagatacaa tcaatgcctg cttttttaca
    10261 tagatattgg aaggtgcgac tatagccagc acaaacggat ttcttatcta atagcacact
    10321 ggtaattcct tgacttctcc acgtgacaga tttggtctgc agggcttcaa ggtcgtaatc
    10381 cgtttgcttt ataatcacct cgtaaaaata tttaactttc tcatagtccg acccttttgg
    10441 tgtctgagca agtatcttat tcacttcatc ttctatttta ttttgtgtcg gagataaatc
    10501 actcggatac accggctctt ctaaatccaa tatatcaatt ccaccagcca ttgctgcttc
    10561 atctgttagc cagtaaaatt ctggataatc atgggcgaca gaaaaatata cgcgcgaata
    10621 tatttcatca ttaacagaat caatatcgat cacttgcatc cgcttgctga gtccattaac
    10681 aaattggaga tagacacgac gttccttatc agtatccaat tgttggtagt aaaaatttcc
    10741 ttgaacctct tcttttaatg ctgttaattc tgcttctagt tgcttttttt ctttagaaaa
    10801 ttcaatttta gctgcttcca aacgttcact cagctcttgt cctcctcctg aacaagcaga
    10861 aaggactgtc gccgataata atatggttaa aacgccacga gaaactttcc tcatatttac
    10921 ctccattctt gtttaagtat aacataatta ggaggcatct gacaaactac acaaaacaaa
    10981 aagggagtgg aaagaattca atagatcgaa aaagaattcc cctcacttcc aaacatttag
    11041 gttcgatttt ctatggtcac tttcttattt ttaggtgacc agctgaaaca gttccccaaa
    11101 cctttggagg ctggagatag agcgaacaga gttcgctaca aaactatctg ggagacagtt
    11161 ggaagttgga aatagagcga actttgagaa gaaaggctgg attttttacc cagcctctaa
    11221 tcttgatgaa tggtcagtta tttgggtaaa cgtacctcaa aaatagttcc tttcggttgg
    11281 ttatctctca cagaaatctc accatctaaa ttcttaataa tttgttgagc taaagataaa
    11341 cctaaaccaa atccaccttt ttgacgtgtt ctagccttgt caactcggta aaagcgatca
    11401 aagattttct ttttatcggc atcgctgatt cccaaaccat tgtcaataac tgtaaaacaa
    11461 actagtttat ccttgatatt agctgccatt tgaattgtcc catcatcatc ggtatacttc
    11521 atagcgttgt caaacaaaat ggtgagcaac tgtttaatca aaactttgtc tgttcgaatt
    11581 ggctgatgaa tcaaattatt aactgttaga gtttttccat tttcttcggc aatcataagg
    11641 taattagcaa agatttcatc aaaatagttg ggttggacat cgatcatatc gacttttaag
    11701 ccatcatcac gacgagccaa attaagtaaa tttgttgtca atagtcgcat atttcgaact
    11761 tcttctaaac tagatccgat gctttcacta ctttccaaaa tagttgtctc gggatgacga
    11821 aatagacttt ccaggcgatt ttgaagaact gccaacggtg tgcgtaactc atgactagca
    11881 ttttcaacga agtctttttg tttttgataa ctaattagga ttggacgcat gctaagattc
    11941 gataggtaaa tacttgctag tatagaaatc aaccaagctg ataccataac aatagcaact
    12001 gtcgattcat agctctcaat ggaagattta atttggctga cattcaccaa aattgtcgca
    12061 tattttatat cataggacga ataataccct agttcatctg tagccagccc aatcgttata
    12121 tagcgataat cttcagacat accaaaactt gattcaaccg tagtttcctt aatttcaccc
    12181 aatttttcct tgtctaatga taggtcggcc aaaccagtaa aagtatctgg attaatcatc
    12241 tctccacttt tactatagag taaaacatga gtattggcac ctaacctcag ctgatctcca
    12301 gctctagtat ttttttgatt tttaggaaca ggcgtgctat ctgaactaga gctcgtagtt
    12361 tcttcaccag ttggactatc ttgaaggata aatacagaat tgggctcata ggttcttgcg
    12421 attgcaaaac caacagccag cgctggttct ttcttaatgc gtttcaaggt gttatctgaa
    12481 ttttggtaca tagttgaacg cattaattga aaaatgatgg ctgtcattaa accaaaaatc
    12541 agagtaaata ctgcaaaata acggataaag aaagaaaatt tatccgtgta cattaatttt
    12601 ttaaatcgct taggcattt
//
LOCUS       DASFSW010000056        12427 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.056, whole genome shotgun sequence.
ACCESSION   DASFSW010000056 DASFSW010000000
VERSION     DASFSW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.056"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1767)
                     /locus_tag="U1099_001141"
     CDS             complement(<1..1767)
                     /locus_tag="U1099_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEM4867467.1"
                     /translation="MKQASYPIQGMTCASCALTVEKAVGKLIGIEEASVNLATEKLSV
                     SYDEKLLGLEDIRQAVEKAGYQLVDNLVTESYDISGMTCASCALTVEKALGKLEGVEE
                     VSVNLATEKATIRYSRHRQNPASLERAVEQAGYQLIRPEKVEGAADKGPSKEEKLWHR
                     FVWSAAFTLPLLYIAMGPMLPWGGLPLPALLHQPLVYAISQVILLIPILYIGRSFFQK
                     GFKTLLQGHPNMDSLIAVGTGAALVQGLLMIAFLLMGKEVAMHGHHPELYFESAAVIL
                     TLITLGKYFEARAKGQTSEAIKKLMNLAPKTAQVLRNGQEIQVPIEEVVVGDQVVVRP
                     GQQIPVDGQVLEGQTRVDESMLTGESLPVKKALGDNVFGGTLNQQGAITMQATKVGRD
                     TTLAQIIRLVEEAQGSKAPIAKLADQVSAIFVPVVMGLALLSGLAWYFLGQESWIFSL
                     SIIIAVLVIACPCALGLATPTAIMVGTGKGAENGLLFKSGQAIETLQGVNTIVFDKTG
                     TITEGKPQVTDVHLLSTKNREQVLQLAASSEQFSEHPLAQALLQAAQTEKIDLLPATD
                     FQALSGRGLSVIIAEQTIYLGNE"
     gene            complement(1888..3711)
                     /locus_tag="U1099_001142"
     CDS             complement(1888..3711)
                     /locus_tag="U1099_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEM4867468.1"
                     /translation="MKKTFVRIATFLMLVCLFPVQLVQACSGFIIGKGLTTDGSILYG
                     RTEDYPYPPDNGAHNKNYIVVPAAAHAKGDMLVDESFGFTAPHLANEFKYTSTPDAAR
                     GDGSNGNFGAHGFNEKGVSMTATVTAIPNKKVLAVDPLVTAGGLGEAILIDYVLPRVT
                     SAREGIELIAKTIDEKGSAEGNIIVIADKNEVWYMEILSGHQYVAIKFPEDKYAIFAN
                     TYYLGHVDFTDTENVIASAKVEEVAKQAENYMMVDGKFHIAKSYGPENYADGDRSRTY
                     AGIKLLDPASSVTYEDAVYDLLRQPTDPSRRFSLQDTFALQRNRFEHLPEFRPDDEAG
                     KVKQGDNGANDQAADATYKYALGNENVIDAHVYQINSSLPSAFGGTVWLGLAQTRNTP
                     YVPFYGIVTDTYEAFKNRSASYDTNSWYWTVQNIDKMAISHPELFGTSIQEKWIALEK
                     EWIASQAALDAQYAGLSEDAAVALAPTVTEATLARSAEIFAQLKAVEAEMMAKIEAAT
                     TPSSSSTEPSTSTEPSSSGTETSTSTSQSTSDSNTGGATDTSSSSGTVVPSDKKVTPT
                     NKKGKSNLPSTGEQVSILLVALGVAGILTAIFLHRKKSSKE"
     gene            complement(3905..4765)
                     /gene="tehB"
                     /locus_tag="U1099_001143"
     CDS             complement(3905..4765)
                     /gene="tehB"
                     /locus_tag="U1099_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEM4867469.1"
                     /translation="MTVLVPYKRMPIWNQDTVPQHFLTKHNTKVGTWAKIKVLRGQLK
                     FEPISDDNEILGEYVYGPTADIPFVEPQAWHRVTLLTEDTEFFLEFFCQPEDYFAKKY
                     DYTRTHSEVLEALKRIEPCKVLDLGCGHGRNSLYLAQQGFEVTAVDKDVPSIRTLLQV
                     KEVEDLDLQAGTYDINSASLEQDYDWIISTVVFMFLERDRVPAIIRNMQEQTRPGGYN
                     LIVAAMDTEDAPCPMNFSFTFGVGELKEYYRDWELVKYNEDFGQLHKRDENGHFLKMR
                     FATMLARKKG"
     gene            4919..5962
                     /locus_tag="U1099_001144"
     CDS             4919..5962
                     /locus_tag="U1099_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEM4867470.1"
                     /translation="MKTAIYEKSGKMTVAEIEKPSLQAKDDVIIKVVRACVCGSDLWG
                     YGEDIHHDHGDTNSGHEAIGIVEEVGEDITTLVPGDFVIVPFTHGCGHCDACRAGFDG
                     TCDNHPGYTNWGNNYQSQYIRFHYGNWALVKIPGQPSDYSEGMIKSLLTLADVMPTGY
                     HADRVADVKPGDKVVVIGDGAVGQCAVIAAKMRGASQIVMMSRHTDRQQMALESGATA
                     IVAERGEEGIAKVREILGGGADAALECVGTEASIDQALGVLHNGGRIGFVGVPHYNNH
                     ALGSTFAQNIIIGGGSASVTTYDKEILLDAVLKGDINPGRVFTQTYSLDNIDQAYKDM
                     ANRKTIKAMITVD"
     gene            complement(6049..6516)
                     /gene="smpB"
                     /locus_tag="U1099_001145"
     CDS             complement(6049..6516)
                     /gene="smpB"
                     /locus_tag="U1099_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEM4867471.1"
                     /translation="MAKGEGNVIAQNKKARHDYTIVDTVEAGMVLTGTEIKSIRAGRI
                     NLKDGFAQIKQGEAWLVNVHIAPYDEGNIWNQEPTRTRKLLLRKKQIESLGNEVKGTG
                     MTLVPLKVYIKDGFAKVLIGLAKGKHDYDKRESIKRREQDRDIKRTMKAINSR"
     gene            complement(6519..8888)
                     /gene="rnr"
                     /locus_tag="U1099_001146"
     CDS             complement(6519..8888)
                     /gene="rnr"
                     /locus_tag="U1099_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEM4867472.1"
                     /translation="MKNDIINYIKEVGPVTMDQLADQFGASSAKSFTDLVKLVSSMEG
                     SRQLVFDNAGRITLPAPKISKNKVTLQGIFRAHKSGFGFVTIDEEEDDLFVSRDDVNF
                     AIEGDRVEIAIKKVADRLKGTAAEAEVIDILEHSLKTAVGLIVFDEDKPEYAGYIKSK
                     NQKIAQKIYIKKSPLVLTGTEILKVDIEAYPNKKRDHFVATIRDVVGHKDDVGIDVLE
                     VLESMDIVSEFPDEVLAEANRVPESPSEQDFAGRLDLRDEIIFTIDGADAKDLDDAVH
                     IKQLKNGNLELGVHIADVSYYVTEGSALDQEAIKRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNVDRLTQSAIMEIDRKGKVVKHWIGQTVIKTNFRMTYSDVNDMIAGNQEKLDKY
                     KTIVPSVELMVKLHETLETMRYKRGALNFDTAEAKIIVNKDGLPVDIQLRQRGIAERM
                     IESFMLVANECVAEHFAKLDLPFIYRIHEEPKSDKLQKFIDYATSFGLTVYGTASSIS
                     QDALQDLMERVKDEPYADVLNMMLLRSMQQARYSEHNHGHYGLGAEFYTHFTSPIRRY
                     PDLLVHRMVRDYGHNPAEKAEHFEQVIPELAKSSSSLERRAIEAEREVEAMKKAEFMQ
                     EFVGQEFDGVVSSVVKFGLFVELPNTVEGLIHITNLNEYYQFHERTLTLQGEKSGRVF
                     RVGQPIRIKLTRADKMTGEIDFAHVPSELDIVEKALKAKRRTASHSNRDRDDRSGRGR
                     GKHHKQEAAGRDSGRHKSGKDHKSKSGKKDKKKKPFYKEVAKKNKKKRR"
     gene            complement(9319..9552)
                     /gene="secG"
                     /locus_tag="U1099_001147"
     CDS             complement(9319..9552)
                     /gene="secG"
                     /locus_tag="U1099_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEM4867473.1"
                     /translation="MYQALLAILLILSVILIVVIFIQPAKNQSSNVFDSSSGALFERT
                     KARGFEAVMQRITAILVFLWMLDALALVIISSK"
     gene            complement(9599..9748)
                     /gene="rpmG"
                     /locus_tag="U1099_001148"
     CDS             complement(9599..9748)
                     /gene="rpmG"
                     /locus_tag="U1099_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM4867474.1"
                     /translation="MRVKINLQCSECGSKNYLTSKNAKTHPDKIEVLKYCPKERKVTL
                     HLETK"
     gene            complement(9748..10953)
                     /locus_tag="U1099_001149"
     CDS             complement(9748..10953)
                     /locus_tag="U1099_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM4867475.1"
                     /translation="MDDGSSYWKQNLKVAWLGNFLTGTSFTLVMPFISVFVEELGVGP
                     GQVEYYAGLAVSVNALAAALMAPIWGSLADRYGRKPMMVRAAFAMIFTMGGMAFVPNV
                     FWLLVLRVLNGVFTGYIPNATALIASQVPKDKTGYALGTLSTGAVAGNLIGPTLGGIL
                     AEMFGVHMVFLLVGLLYAIVVLLTVFYIREDFVPIKKGEEMSVKEVFEQVKDRQMLVG
                     LFVTSMIIIAAAQAVVPILTLYVRHLGQTDNLLFVAGFIISLPGMASLVTSGYLGKIG
                     DHIGNHRLLLIALTYSLLINVFCVFAENPFQLGLLRFMYGFGTGALLPSVNSLLTKLT
                     PKEGISRIFSYNQLFNNLGSVVGPMMGSAVAAHMGYDWVFYLSSGLVLFNLIWSLTNF
                     RNYLKVRDV"
     gene            complement(10940..11542)
                     /locus_tag="U1099_001150"
     CDS             complement(10940..11542)
                     /locus_tag="U1099_001150"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEM4867476.1"
                     /translation="MAKIIGLTGGIASGKSMVTAFLREQGYSVIDADAVVHELQAKGG
                     KLYQVLVKEFGQDILSDDGNLDRAKLGQAVFADSKLRARLSDLQDQIIRQELLDRRDA
                     LKQTEDVIFMDIPLLYEADYSGEVNEVWLVYVDRAQQLERLMKRNGLAVQDAENRMAA
                     QLSLEEKRSQAQVVIDNSGAVEATLVQVARLLEELKDGRR"
     gene            complement(11535..12362)
                     /gene="mutM"
                     /locus_tag="U1099_001151"
     CDS             complement(11535..12362)
                     /gene="mutM"
                     /locus_tag="U1099_001151"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEM4867477.1"
                     /translation="MPELPEVETVRRGLNRLVKGKIISKVEVTYAPMIKTGVDAFCQD
                     LIGQEILDVDRRGKYLLIYLTDHVLISHLRMEGKYNFFPDQVPANKHFHAFFTFSDGS
                     TLVYQDVRKFGTMELLGKADVDAYFISRKIGPEPTEEDFDLEEFAKKLAKSRKPIKSH
                     LLDQSLVAGLGNIYVDEVLFKAKVHPAQTSNQLSAEQVADLRQATIEVLQLGIEKGGS
                     TIRTYKNALGMDGTMQDYLQVYGKTGQACPRCQTEIVKIQLGGRGTHFCPKCQVKHG"
BASE COUNT         3410 a         3131 c         2534 g         3352 t
ORIGIN      
        1 ttcatttccc agatagatgg tctgctctgc tattatcacc gaaagaccac gaccagaaag
       61 tgcctgaaaa tctgtggcgg gcaagagatc aatcttttct gtctgagctg cttgcaggag
      121 agcctgagcc aagggatgct cggaaaattg ctcgctactt gcggccagct ggaggacctg
      181 ctcacgattt ttagtagaca agaggtggac gtctgtcacc tggggtttgc cctcagtaat
      241 ggtcccagtc ttgtcgaaga caatggtgtt cacaccttgt agggtttcaa tagcttgacc
      301 agacttgaac agaaggccat tttctgcacc ttttccagta ccaaccatga tggcagtcgg
      361 cgttgctaag cccagagcac aagggcaggc gatgaccaag acggcgatga taatgcttaa
      421 tgaaaaaatc catgattctt gacccaagaa gtaccaggcc agaccggaca agagagccaa
      481 gcccatgacg actggtacaa atatggcaga tacttggtca gccaatttgg cgatgggagc
      541 cttagaacct tgagcctctt ctaccaagcg aataatctgg gccagagtcg tatcacgacc
      601 aaccttggta gcctgcatgg taatggcgcc ttgctggtta agggtgccgc caaaaacatt
      661 atcgcctaga gctttcttca ctggtaggct ctcacccgtc agcatagact catccacacg
      721 tgtctggcct tcgagaacct gaccatcaac aggaatctgc tgacctggac gaacaacgac
      781 ctggtcaccg accaccactt cttcaatcgg cacttgtatt tcctgaccat ttcgcaagac
      841 ctgggccgtc ttgggagcca gattcatcaa tttcttaatg gcctcagaag tctgaccttt
      901 agctctagct tcaaaatatt ttcctagggt aatcaaggtc aagataacgg cggctgattc
      961 aaaatacagt tctggatggt gaccgtgcat ggccacttct ttccccatca gaagaaacgc
     1021 aatcatcaaa agaccttgaa ccaaggctgc ccctgtccca acagcaatca aggaatccat
     1081 atttggatgg ccttgcaaga gagttttaaa gcctttttgg aaaaaactgc gtccgatata
     1141 gaggataggg atgagcaaga tgacttggct gatcgcatag actagcggtt ggtggagcaa
     1201 agctggtaat ggaagccctc cccaaggcaa cataggaccc atagcaatat agagcagtgg
     1261 aagagtaaat gctgctgacc agacaaatcg atgccacagc ttttcttcct tgctcggtcc
     1321 cttgtccgca gccccttcta ctttttctgg tctaatcaac tgataacctg cctgctcaac
     1381 tgctctttca agactagctg gattttgcct atgacggcta taacgaatag tcgccttttc
     1441 tgttgccaga ttgacagaga cttcttctac accctctagt ttgcccagag ctttttcaac
     1501 cgtcagagca caggaagcac aggtcatgcc tgaaatgtca taagactcgg tcaccaagtt
     1561 atcgactaac tgataacccg ccttttcaac tgcctgacga atatcctcta gtcccaaaag
     1621 cttttcatca tagctaacac tcaatttttc agtcgctagg ttgacactgg cttcttcaat
     1681 ccctattagt ttaccaaccg ctttctcaac cgtcagagca caggaagcgc aggtcatacc
     1741 ttgaatggga tagcttgctt gtttcatagg ttactcctct ttcgactaca tctgtaaaca
     1801 aattatacaa catatgacta catttgtaaa caaaattgtt tcaaaaaagt agattgaaat
     1861 caatccaacc tacttttaca tattcattta ttctttactg gattttttcc gatgaagaaa
     1921 aattgctgtt aaaattcctg caacacctag cgcaaccaac aagatgctga cttgctcacc
     1981 tgtactagga agatttgatt tgcctttttt attggtaggt gttactttct tatcagaagg
     2041 cactactgtt ccgcttgatg aggaagtatc cgtagctcct cctgtgttag aatcacttgt
     2101 agactgactt gtactggttg aagtttcggt accacttgat gatggctcag tgctggtcga
     2161 tggttcagta ctacttgatg acggagttgt tgctgcttca atttttgcca tcatttcagc
     2221 ttcaacagct tttagttggg caaaaatctc tgctgaacga gccaaggttg cctctgttac
     2281 agttggagcc agagctacgg ctgcatcttc tgaaagtcca gcatattgag catcaagagc
     2341 tgcctgtgaa gcaatccatt ctttttcaag tgcaatccat ttttcttgaa ttgatgtacc
     2401 aaataactct gggtgtgaga tggccatctt atcaatattt tgtaccgtcc agtaccatga
     2461 attggtatca taagaagctg agcgattttt aaaggcttca taagtatcag tcacaatacc
     2521 ataaaatgga acatacggtg tattccttgt ctgagccaag cccaaccata ctgtgccacc
     2581 gaaggctgat ggtaagctac tattaatctg ataaacatgg gcatcgatga cgttttcatt
     2641 accaagagcg tatttataag tcgcatctgc cgcttggtca ttcgcaccat tatccccttg
     2701 ttttacctta cctgcttcat cgtctggacg aaattctgga agatgttcaa aacggttacg
     2761 ttgtaaggca aaggtatctt gtaaactaaa gcgacgactt gggtctgttg gctgacgtag
     2821 caaatcatac acagcatctt cataggttac agacgatgct ggatctaaca attttatacc
     2881 agcgtatgtc cgagaacgat caccatcagc ataattctca ggtccatatg atttggcaat
     2941 atggaacttc ccatcaacca tcatataatt ttcagcctgt ttggctactt cttcaacctt
     3001 tgcagaagca atcacatttt ctgtatcggt aaaatcaaca tgacctagat agtaagtatt
     3061 tgcaaaaatt gcatacttgt cttcagggaa cttgatggct acgtattggt ggcctgacaa
     3121 aatttccatg taccaaactt cattcttatc agcaataaca atgatattcc cttctgccga
     3181 acctttttca tcaattgtct tcgcaattaa ttcgatacct tcacgcgcac tggtgactct
     3241 aggaagaaca tagtcaatca aaatggcctc acctagtccc cccgcagtta ccaatgggtc
     3301 aacagctaaa acttttttat ttggaatagc tgtaacagta gcagtcatgg acacaccttt
     3361 ttcattaaat ccatgcgcac caaaattacc gtttgaccca tccccacgtg ccgcatcagg
     3421 cgtagaggtg tacttgaact catttgccaa gtgtggtgct gtgaaaccga atgattcatc
     3481 caccaacata tcccccttgg catgggctgc tgctggaacc acaatataat ttttattgtg
     3541 cgctccatta tctggtggat agggataatc ttccgttcta ccataaagga tagaaccatc
     3601 cgttgtcaac cctttcccaa taataaagcc tgaacaggct tgaacaagtt gaacagggaa
     3661 taggcaaacc aacattagaa atgtggctat acgaacaaaa gtctttttca tagacttctc
     3721 ctttcctgat actctgtgaa aatcagaagt aggcgccctc cagagatata aactcagaga
     3781 ctattttaca caagtctctt atccaaccag aaggtgattt tcgtggagta taatatgatt
     3841 tacccatttg cacctacatt gtaacgcaaa agcacctgcc ttgcaagtgc tttcaaaaaa
     3901 atatttatcc ttttttccta gccagcatgg tcgcaaaccg catcttgagg aaatggccat
     3961 tttcatcccg cttgtgaagt tgaccgaaat cttcattgta tttgaccagt tcccagtcac
     4021 ggtagtattc cttcaactca cctactccaa aagtaaagga gaaattcata ggacagggcg
     4081 catcttccgt atccatggcc gccacaatca agttatagcc accaggtcgt gtctgctcct
     4141 gcatattgcg gataatggct ggtaccctat cccgctccaa aaacatgaag accacagtcg
     4201 agataatcca gtcgtagtcc tgctccagac tggctgaatt gatgtcatag gtcccagcct
     4261 gcaagtccaa gtcctccact tccttgacct gcaagagtgt ccgaatactt ggcacatcct
     4321 tatcgaccgc cgtcacctca aatccctgct gagccaagta gagactgttc ctaccatgac
     4381 cacagcccag gtccaagacc ttacaaggct caattctctt caaggcctcc aagacttccg
     4441 aatgtgtccg agtatagtca tactttttgg caaaatagtc ttctggttga cagaaaaatt
     4501 ccaagaaaaa ctccgtatcc tctgtcagca aagtcacccg atgccaagcc tgtggctcga
     4561 caaagggaat atcggctgtc ggaccataga catattctcc caaaatctca ttatcatctg
     4621 aaatcggctc aaatttaagt tgacctctca agaccttgat tttggcccaa gtacccacct
     4681 tggtattgtg cttggtcaaa aaatgctggg gaaccgtatc ctgattccaa atgggcatgc
     4741 gtttgtaagg aactaaaaca gtcattatct ctccttttct ttccgataca cttagtctat
     4801 cacaaaggaa atatgaattc aaacccattt ttttcttgac ttggagtgaa ctccaaggtg
     4861 tataatattc catattaatc agaaaggata tttactgaca agtaaatgtg tgatgaatat
     4921 gaaaacagct atttatgaaa aatccggtaa gatgaccgtt gcagaaatcg aaaaaccaag
     4981 ccttcaagcc aaagatgatg tcattatcaa ggtcgttcgg gcctgtgtct gtggatcgga
     5041 cctttggggc tacggagaag acattcacca tgaccacggt gacacaaact ctggtcatga
     5101 agccatcggt atcgttgaag aggtaggcga agacatcaca acacttgttc ctggtgactt
     5161 cgttatcgtt ccatttacac atggttgcgg acactgtgat gcctgtcgtg ctggctttga
     5221 cggtacttgt gacaaccatc caggctacac caactggggc aacaactacc agtctcaata
     5281 cattcgtttc cactacggca actgggcctt ggtcaaaatt cctggtcagc caagtgacta
     5341 ctcagaaggc atgatcaaat ctctcttgac tctggcagat gttatgccaa ctggctacca
     5401 cgcagaccgt gtagcagatg tcaaacctgg tgacaaggtt gttgtcatcg gtgatggtgc
     5461 agtgggtcaa tgtgctgtaa ttgctgcaaa aatgcgcgga gcttctcaga ttgtcatgat
     5521 gagccgccat acagaccgcc aacaaatggc tttggaatca ggggctactg ccatcgtagc
     5581 agaacgtggt gaggaaggaa ttgccaaagt ccgtgaaatt cttggaggtg gcgctgatgc
     5641 tgccctcgaa tgtgttggta cagaagcctc tattgaccaa gccctcggcg tgcttcacaa
     5701 tggtggtcgt atcggttttg tcggtgtacc acactacaac aaccacgccc ttggctcaac
     5761 ctttgctcaa aatatcatta tcggtggtgg ctctgcttct gtcacaacct atgataagga
     5821 aattttgctc gatgccgttc tcaaaggcga catcaaccca ggtcgagttt tcacccaaac
     5881 ttacagcctg gacaatatcg accaagccta caaagacatg gccaaccgga aaacgattaa
     5941 agctatgatt acggtagatt aagataaata ataagcccca gatagtctcg aaactgtctg
     6001 gggtatttgt tttccgataa caaaaaagac caggatttct cccagcctct atcgactatt
     6061 gatagccttc atggtccgtt tgatatcacg gtcttgctca cggcgtttaa ttgactcccg
     6121 cttgtcgtag tcgtgcttcc ctttggcaag cccaatcaaa accttggcaa agccatcctt
     6181 gatatagacc ttgaggggca ccagagtcat ccctgttccc ttgacctcat tgcccagaga
     6241 ctcaatctgc ttcttccgca agaggagctt gcgggtccgc gttggctcct gattccagat
     6301 attgccctca tcgtagggag cgatatggac attgaccaac caagcctcgc cctgcttgat
     6361 ctgggcaaag ccgtccttaa ggttgatccg ccccgcacga atagacttaa tctccgtccc
     6421 cgtcaggacc atccccgcct ccactgtatc gacaatggta tagtcgtggc gggctttttt
     6481 attttgcgca atcacattgc cctcaccctt ggccatgcct atctcctttt cttcttgttc
     6541 tttttagcca cttctttgta aaatggtttc tttttcttgt cttttttgcc actttttgac
     6601 ttgtggtctt ttccagactt gtgacgtccg ctgtcgcgac cagcagcttc ttgtttgtgg
     6661 tgcttgcccc gtcctcgccc tgaccgatca tcacggtcac ggttagagtg actagcagtt
     6721 ctccgtttgg ctttcagagc tttttcgaca atatccagct cggacggcac gtgggcaaaa
     6781 tcgatttccc ctgtcatctt gtccgcccgc gttagcttga tacgaattgg ctgaccgaca
     6841 cggaagaccc gacctgattt ctcaccctgc aaggtcagag ttcgctcgtg gaactgatag
     6901 tattcgttga ggttggtgat atgaatcagg ccttcaaccg tgtttggtaa ctctacaaaa
     6961 agaccaaact tgactacgct agatacgaca ccatcaaact cttgcccaac aaattcttgc
     7021 atgaactctg ccttcttcat ggcttcgact tcccgctctg cctcaatggc acggcgttct
     7081 aaactggacg aagacttggc aagctcaggt ataacctgct caaaatgctc tgctttttcc
     7141 gcaggattat gaccatagtc ccgcaccatg cggtgaacca gcaagtcggg ataacgacgg
     7201 atagggctgg taaagtgggt gtagaactcg gcacccagac cgtaatggcc gtggttgtgc
     7261 tccgagtagc gtgcctgctg catagaacgc agcaacatca tgttgaggac atccgcatag
     7321 ggttcatcct tgacccgctc catgaggtcc tgaagggcgt cctggctgat agagctggcc
     7381 gtaccataga ccgtcagacc aaagctggtc gcatagtcga tgaacttttg cagcttgtct
     7441 gacttaggct cctcgtggat ccgatagatg aaaggcaggt ccagtttggc aaaatgctca
     7501 gcaacgcact cattggcaac cagcatgaag gactcaatca tccgctcagc aatgcctcgc
     7561 tgacgtagct ggatatcgac tggcagacca tccttgttga cgatgatttt agcttcagct
     7621 gtatcaaagt tgagagcccc acgcttgtag cgcatggttt ccagggtttc atggagtttg
     7681 accatgagct caacacttgg cacgattgtc ttgtatttgt ccagcttttc ctgatttccc
     7741 gcaatcatgt cattgacatc agaataggtc atgcggaagt tggttttgat gaccgtttgg
     7801 ccaatccagt gcttgacaac cttgcccttg cggtcaattt ccataatagc cgactgggtt
     7861 agacggtcca cgtttggatt aagagagcag atgccgtttg acaaacgttc tggcaacatt
     7921 ggcaccacac ggtcggtcac atagacagac gttccacgct tgatggcctc ttgatccaag
     7981 gcagagcctt ccgtcacata gtagctgaca tcagcgatat ggacacctag ctccagattg
     8041 ccgttcttaa gctgcttgat atggaccgca tcgtccaagt ccttggcatc cgcaccgtct
     8101 atggtgaaga taatttcatc tcgcaggtcc aaacgtcctg caaagtcctg ttcagacgga
     8161 ctttctggta cccgattggc ctctgccaag acttcatctg gaaactccga cacgatatcc
     8221 atggactcca aaacttccaa gacatcgatg cccacatcgt ccttgtggcc caccacgtcc
     8281 cgaatggtcg caacaaagtg gtcccgtttc ttgtttggat aagcttcgat atcaactttt
     8341 aggatttccg ttccggtcaa taccagaggt gacttcttga tgtaaatctt ctgagcaatt
     8401 ttctggtttt tagacttgat gtagcctgca tattctggct tatcttcgtc aaagacaatc
     8461 aagcccaccg ctgttttcag gctatgttcc aaaatatcaa tgacttccgc ttccgcggca
     8521 gttcccttga gacgatctgc aacctttttg attgcaattt caactctgtc accttcgata
     8581 gcaaagttaa catcgtcacg gctgacaaag agatcatctt cctcctcgtc aatggtcaca
     8641 aagccaaaac ccgacttgtg ggcacggaaa ataccctgta aggtcacctt gttcttggag
     8701 atttttggag caggaagagt aatccgccca gcattgtcaa agacaagttg acgactgcct
     8761 tccatgctag aaaccagctt gaccaagtct gtaaagctct tagcagaact ggctccaaac
     8821 tgatctgcca gctggtccat ggtcacagga ccaacttctt taatatagtt aataatatcg
     8881 tttttcatgt tgtatttacc agagtgcagg ataaaaacct agcactattt ctctttcagt
     8941 cctttagttt atcttttatt actgcgacgt acgaggaaac tccgtttccc tatttccaac
     9001 ttcaaataat ttcatcatta tttgaactcg ctttgtcgta cttcagtaca gtctgcagct
     9061 cgttgcctag tactaaaagc aaactaaaag actgtatttt gcgttatatc cagacgcagt
     9121 ggttgattgc tccaacaatc aaatgattgt tggaggtggg agataggatt tgcaaagcaa
     9181 atctcaatag ttcgcgaata acgctccgaa ccatacctat tcaacttgtg cggggtaggc
     9241 agacgaactc tttatacttg tcgagttctg gcccactccc aaaaaaatag accaagaaca
     9301 agtcccagtc tgatttatct acttacttga aatgataaca agcgccagcg catccagcat
     9361 ccaaagaaaa acaagaatag ctgtaatgcg ttgcatcacc gcttcaaaac cacgggcctt
     9421 tgtacgttca aataaggctc cactagaaga gtcaaagacg ttgctagact ggtttttagc
     9481 cggttgaata aaaatcacta caatcaaaat aacagataaa atcaacagaa ttgctaataa
     9541 tgcttgatac atattttttt cactttctac aataacttat gacattatac cataaaacct
     9601 acttagtttc aaggtggagg gttacttttc tctcttttgg gcagtattta aggacttcaa
     9661 tcttatctgg atgagtcttg gcgtttttgc tagtcaagta gttcttacta ccacattctg
     9721 aacattgtaa attgatttta actctcacta aacatccctt actttcaaat agtttctaaa
     9781 attggtcaaa gaccagataa ggttaaagag gacaagtccg ctcgacagat agaaaaccca
     9841 gtcataaccc atgtgagcag ctaccgcaga ccccatcatg ggaccaacaa cagaacctag
     9901 attattaaag agttggttgt aggaaaatat ccttgaaatc ccttctttgg gggtcaactt
     9961 ggtcaaaaga gaattgaccg aaggcaacaa tgctcctgtc ccaaaaccat acatgaatcg
    10021 caacaaacca agctggaaag gattttctgc aaaaacacag aaaacattaa tcaacagact
    10081 gtaagtcaaa gcaattaata gaagtcgatg attaccaatg tgatcaccaa ttttccctaa
    10141 ataacctgat gtcactagcg atgccatacc aggcaaggaa atgataaaac cagcaacaaa
    10201 taacagatta tctgtctgtc ccaaatgccg cacatagagg gtcaatatag gaacgacggc
    10261 ctgagcagct gcaataataa tcatagatgt cacaaaaagc ccaaccagca tctgccgatc
    10321 tttcacctgc tcaaatacct ctttaaccga catttcttcg ccttttttga ttggaacaaa
    10381 atcctctcga atatagaaaa cggtcagtaa cacaacgata gcataaagaa gtcccaccag
    10441 caaaaatacc atatgtacgc caaacatctc tgctaagata ccacctaggg tcggaccaat
    10501 caaattaccc gctaccgcac ctgttgataa ggtacccaga gcataccccg tcttatcctt
    10561 tggaacctga ctggcaatca gagctgtcgc atttggaata taacccgtaa agacaccgtt
    10621 aagtacacga agaacaagaa gccagaaaac attgggaaca aaagccattc cacccatggt
    10681 aaagatcata gcaaaagccg cccgaaccat cataggcttg cgcccatagc ggtcagccaa
    10741 actcccccaa ataggcgcca tcaaggctgc tgcaagggca ttaacagata ccgcaagccc
    10801 cgcataatat tctacctgtc cagggcccac tcccaactct tccacaaaaa ccgagataaa
    10861 aggcataacc aaggtaaaac tcgtccctgt taggaaattt cccaaccaag ccaccttcaa
    10921 attctgcttc caataactac taccgtcgtc catcctttaa ttcctccaaa agccgagcaa
    10981 cctgcactaa ggtcgcctca accgctccgc tattgtcaat caccacctgg gcttgcgacc
    11041 gtttttcctc taaagaaagc tgagcagcca tacgattttc tgcatcttga acagctaagc
    11101 cattgcgttt cataagccgt tccaactgct gcgctctatc gacataaact agccagactt
    11161 catttacttc cccgctataa tccgcctcgt agagcaaagg aatatccata aaaatcacat
    11221 cctccgtctg cttgagggca tcccttctgt ctaacagctc ctgcctgata atttggtcct
    11281 gcaggtcaga caggcgagcc cgcaacttgc tatccgcaaa aacagcctgg cccagcttag
    11341 ccctatccaa atttccatca tctgataaga tgtcttgacc aaactctttc accaaaacct
    11401 gatagagctt gccacctttg gcctgtaatt cgtgaacaac cgcatccgca tcgatgactg
    11461 aatagccctg ttctcgcaaa aaagctgtga ccattgattt tccagaggcg attccgcctg
    11521 ttaaaccgat aattttagcc atgtttcacc tgacacttgg gacagaaatg tgtcccccgt
    11581 ccgcccagct ggattttgac aatctctgtc tggcaacggg gacaggcctg ccctgtcttt
    11641 ccgtaaactt gtaaataatc ctgcatagtc ccatccatgc ccaaggcatt tttgtaagtc
    11701 cgaatggttg aaccaccttt ttcaattccc agttgtagaa cttctatggt agcttggcgg
    11761 agatctgcca cctgctctgc tgatagctga ttactcgttt gtgcaggatg aactttggcc
    11821 ttgaacaaga cctcatcgac ataaatattg cccaaaccag ccaccaaaga ctggtccaaa
    11881 agatgcgact taatgggttt tctcgactta gctaactttt tggcaaattc ttctaagtca
    11941 aaatcttcct ccgtcggctc aggaccaatt tttcgactga taaaataggc atccacatct
    12001 gccttgccca gcagttccat tgtgccaaac ttgcggacat cttgatagac caaggtcgag
    12061 ccatctgaaa aagtaaaaaa ggcatggaaa tgtttgttgg caggcacctg gtcaggaaag
    12121 aaattgtact taccttccat ccgcaaatga gaaatgagga catggtctgt caggtaaatc
    12181 aagagatact tgccacgacg gtccacatct aaaatctcct gaccaatcaa gtcctgacag
    12241 aaggcatcca cacctgtctt aatcatggga gcataggtca cctccacctt ggaaataatt
    12301 tttcccttga ccaaacgatt caaaccacga cgaaccgtct caacctcagg caattcgggc
    12361 aatgtacgat accaagagcg taaaaagcga ctgaaaatta ggagagcgac gctgtgtgac
    12421 ttgcaca
//
LOCUS       DASFSW010000057        12400 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.057, whole genome shotgun sequence.
ACCESSION   DASFSW010000057 DASFSW010000000
VERSION     DASFSW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.057"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(43..738)
                     /locus_tag="U1099_001152"
     CDS             complement(43..738)
                     /locus_tag="U1099_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM4867478.1"
                     /translation="MISNEQYDYLRAHPTFERLTRDSFDQLAKHIRFRKIPKGQIFFY
                     AEDPRDYLFVLYKGYARIEQYDETDSFTYLDYIRQGGAFPFGDMFQDKPYHYTAIAIT
                     DLEYFIVPMVLFETLSKINPSQMVYICQKLSKVLRFQELRLRNAMRSKASDRVVQALA
                     LLYWDMCQRDQFATLPFEIHIQEISRLAATTRETVSHVLKQLKQDGKIAYSHKQLTYL
                     DIDYFLENLTETH"
     gene            complement(1022..1939)
                     /gene="whiA"
                     /locus_tag="U1099_001153"
     CDS             complement(1022..1939)
                     /gene="whiA"
                     /locus_tag="U1099_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEM4867479.1"
                     /translation="MSFTVQVKEELLLQSSQNKSELSAMIKLSGSLGLASSGLTLSIS
                     TENAKIARHIYELLLHFYQIKAEIRHHQKPNLKKNRVYAVLIEDGVNEILNDLHLADS
                     FFGLETGISPLVLENDSWSQAYLRGAFLAAGSVKDPEKGKYQLEIASVYSDHANDLAN
                     LMQKFLLDAKVIERSKGTITYLQRAEDIMDFLLVIGAEETKTEFENVKLLREARNDLN
                     RATNAEAANIAKTVNASMKTINNIIKIMDTIGLDQLSGDLQEIAQLRIQHPDYSIQQL
                     ADSLTVPITKSGVNHRLRKINKIADELTE"
     gene            complement(1936..2910)
                     /locus_tag="U1099_001154"
     CDS             complement(1936..2910)
                     /locus_tag="U1099_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEM4867480.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRDKDVEITAIVTVADDGGSSGE
                     IRQALQVTPPGDLRNVLLAMSDMPKLYEQIFQYRFADSDGPLAGHPLGNLIIAGISEM
                     QGSTYNAMRLLTRFFHTTGRIYPSSEQALTLHAIFTDGTEVAGESKISKHNGMIDHVY
                     VTNSYNDDEPKASRQVVETIMESDMIVLGPGSLFTSILPNLMISDIGKALKETKAEVT
                     YVCNIMTQRGETEFFSDADHVAVLNAHLAEQFIDTVLVNIEPVPQEYMNSNQFDEYLV
                     QVKHDFAGLQKQANRVISSNFLRLENGGAFHDGDLVVEELLKILQVRP"
     gene            complement(2907..3794)
                     /gene="rapZ"
                     /locus_tag="U1099_001155"
     CDS             complement(2907..3794)
                     /gene="rapZ"
                     /locus_tag="U1099_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEM4867481.1"
                     /translation="MSDKLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLLPKF
                     LELIRHSQDNNKIALVVDMRSRSFFSEIREVLDEIEGAEDLDVKVLFLDATDNELVAR
                     YKETRRSHPLAADGRVLDGIQLERELLAPLKNMSQNIIDTTELTPRNLRKAISEQFAS
                     QDNQPSFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKLELRNLTGLDAPVFDYVME
                     HQESEEFYSHLLGLIEPILPGYQKEGKSILTIAVGCTGGQHRSVAFAKRLADDLEKNW
                     NVNRSHRDKDRRKETVNRS"
     gene            complement(3816..4193)
                     /locus_tag="U1099_001156"
     CDS             complement(3816..4193)
                     /locus_tag="U1099_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEM4867482.1"
                     /translation="MKTIHTDKAPAAIGPYVQGKVVGNFLFASGQVPLSPETGEVVGE
                     TIQEQTEQVLKNIAAILSEAGTDFDHVVKTTCFLKDMNDFVAFNKVYKTAFSADFPAR
                     SAVEVARLPRDVKVEIEVIAVID"
     gene            complement(4377..5696)
                     /locus_tag="U1099_001157"
     CDS             complement(4377..5696)
                     /locus_tag="U1099_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEM4867483.1"
                     /translation="MKQESLGKEIIRLSLPATVENIFQTLVGFVDTLLIAQLGLVAVT
                     AVGLANTILNVYLAVYMALGVGATALIARSIGAGDRESLTFHVRQALVLSVGVGVLFG
                     LLSLVFGRQMLVLMGADAESLAGAQAFFYWVGGLTIFQALMTILGTILRASGDTVSPM
                     KMSLLTNGFNVVLDYFLIFGIGSWSGLGIVGTALGTVLARLLGTVLLYRKVQQTDLAV
                     DLKQLFHLGSPKEMVDLTLPAAAERLVMRLGQVVYFSLIVGLGTTVYASHMIAGNIES
                     FTYMPAYGLATAAAVLIGQALVKGDILTVRRVAFLSSAYGVAIMFLLGTILFFGAPSF
                     ALLFTKDLEAVHQVVTALRIDAFNQPGLAVSLNMAGALQGLGDTKSPLYSTVIGMWGL
                     RVVGAIVLGQMFGLGIAGVWLSILIDLLLRAIFLTWRFHVKTRKLAE"
     gene            complement(6102..6371)
                     /locus_tag="U1099_001158"
     CDS             complement(6102..6371)
                     /locus_tag="U1099_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM4867484.1"
                     /translation="MAYKLVLSDDALKQLKKMDRHVGMMLAKDLKKRLDGLENPRQFG
                     KALVGDYKGLWRYRVGNYRVICDIIDNKMVILALEIGHRKEIYKK"
     gene            complement(6361..6588)
                     /locus_tag="U1099_001159"
     CDS             complement(6361..6588)
                     /locus_tag="U1099_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation repressor RelB"
                     /protein_id="HEM4867485.1"
                     /translation="MGIVSLRLNDAEEEIFRSYAIHTGKSLSELFKTALAEQIEDQLD
                     YEIGIKALKKFQENPVTHSIDDVLAELEDGL"
     gene            complement(6860..8206)
                     /gene="asnS"
                     /locus_tag="U1099_001160"
     CDS             complement(6860..8206)
                     /gene="asnS"
                     /locus_tag="U1099_001160"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEM4867486.1"
                     /translation="MSRKLITINQVKDYVGQEVTIGAWVANKSGKGKLAFLQLRDGTA
                     FFQAVALKPNFIEKFGEEAGTEKFDVAKRLSQETSVYVTGIVKEDERSKFGYELDVTD
                     LEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAIQQIRNAIIYATYEFFEKNGF
                     IKFDSPILSGNAAEDSTELFETDYFGQPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVIDRAPQALETLERDVD
                     ALKRYIAEPFKRVAYDDAITLLQEHEADEDTDYEHIEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYEFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRLHP"
     gene            complement(8221..9402)
                     /locus_tag="U1099_001161"
     CDS             complement(8221..9402)
                     /locus_tag="U1099_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM4867487.1"
                     /translation="MSKLSRRVLEMEESVTLAAGARAKALKAEGRDILELTLGEPDFV
                     TPKNIQEAAIKSIEDGKASFYTVASGHPELKDAVNTYFENFYGYSIERNQVVLATGAK
                     FVLYAFFAAVINPGDEVIIPTPYWVSYADQIKMNDGVPVFVTAREEHNFKVTVDQLEA
                     ARTDKTKVFLLNTPSNPTGMIYSREELEAIGNWAVEHDILILADDIYGRLVYNGNGFT
                     PISSISESIRQQTIVVNGVAKAYAMTGWRVGFAVGNPEIIAAMSKIVGQTTSNLTTAA
                     QYAAIEAFTGPQDTVETMRQAFEERLNTIYPLLAEVPGFEVIKPEGAFYLFPNVKKAM
                     EMKGYTDVTEFTTAILEEVGVAMVTGAGFGAPENIRLSYATDMDTLKEAVARLHTFMK
                     K"
     gene            complement(9399..9896)
                     /locus_tag="U1099_001162"
     CDS             complement(9399..9896)
                     /locus_tag="U1099_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEM4867488.1"
                     /translation="MEKKIFQLLEWMASKTGQLVLGSFILLSVVTFSIFTIWDIAAAP
                     FNNVRSHAVAVATEYADLQTVNDFSIYNGTETYFCVFGVTSQGEEVAVLIPEASSTVY
                     VYPLVQGISQEEAQAIAKKNGASQVERTILGLRDGKPIWEVKSGTAYYLVEFETGNFI
                     KREGL"
     gene            10199..10648
                     /locus_tag="U1099_001163"
     CDS             10199..10648
                     /locus_tag="U1099_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM4867489.1"
                     /translation="MRTVEQSLNENKHALHSLVVFRRAANTIAKSELETIKKYGLTVC
                     QFGVMEALYNKGNLRIQDLIDKLLSTSGNMTVVIKNMIRDGYIYKTIDTNDRRASLIA
                     LTPLGRETIEKILPEHYDHVGEIFSVLSSEEQDQLAEILKKFKNQQT"
     gene            10711..11256
                     /locus_tag="U1099_001164"
     CDS             10711..11256
                     /locus_tag="U1099_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM4867490.1"
                     /translation="MANVLFLVGSLRDGSFNHQMAKKAEALLTDKAQVSYIDLAKIPL
                     FNQDIETPILPEIAELRAQVDAADAIWIFSPVYNYAIPGIVKNALDWLSRSLDLSNPK
                     GPSILQDKITTVSAVANSGHDHLFKDFQHLLPFIRTQIAGEFTGSTINPEAWGTGVLE
                     LSDETLTKLEQQVADLLAAIQ"
     gene            complement(11355..>12400)
                     /gene="glf"
                     /locus_tag="U1099_001165"
     CDS             complement(11355..>12400)
                     /gene="glf"
                     /locus_tag="U1099_001165"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEM4867491.1"
                     /translation="KKGKKIKVIEKRDHIAGNIYTKEVEGIQVHEYGAHIFHTSEKEI
                     WDYVNQFAEFNSYTNTPVANYKGEIYNLPFNMNTFNKLWGVVTPAEAEAKIAEQRAVL
                     GGKTPENLEEQAISLVGTDIYEKLIKSYTEKQWEKPCTELPAFIIRRLPVRLTYDNNY
                     FNDTYQGIPIGGYTQIVEKMLEHENIDVETNVDFFANKEDYLATYPKIVFTGMIDEFF
                     DYQLGELEYRSLRFETEVLDMENYQGNAVVNYTDSETPYTRIIEHKHFEFGTQPKTII
                     TREYSKTWKRGDEPYYPVNNNRNNYLYTVYKKLSEQQENVIFGGRLGHYRYYDMHQVI
                     GAALQCVRNEVGE"
BASE COUNT         3703 a         2923 c         2272 g         3502 t
ORIGIN      
        1 cattatatga atttttattt ttttataaaa aaaacagaaa cttcaatgag tttctgtcaa
       61 attttctaag aaataatcaa tatctaaata agttaactgt ttatggctat aagcaatttt
      121 accatcctgc ttcagttgct tcaaaacatg gctaaccgtt tcccgtgtcg tagctgctaa
      181 acgcgaaatc tcttgaatat gaatttcaaa tggcaaggta gcaaactgat cgcgctggca
      241 catatcccaa taaagaagag ccaaggcttg aacgactcga tctgatgcct tggaacgcat
      301 cgcatttcgc aagcgcaact cttgaaaacg aagcactttg gataattttt gacagatata
      361 aaccatttga ctcgggttta tcttcgacaa ggtttcaaac aagaccatcg gtacaataaa
      421 atactctagg tccgttatgg caatggcggt ataatgataa ggtttgtcct gaaacatatc
      481 tccaaaagga aaggcaccac cctgccgaat atagtccaaa tacgtaaaac tatccgtctc
      541 atcatattgc tcaatccgag cataaccctt atacaataca aatagataat ctctaggatc
      601 ctctgcgtaa aaaaagattt gccccttagg aatctttcga aaacgaatat gcttagctaa
      661 ttgatcaaag ctatcacgtg tcaaacgttc aaaagttgga tgtgccctta gataatcata
      721 ttgttcgttg cttatcatca aaactccttt ctagctaaag tatccttcta tctattttaa
      781 catatattaa cgttctatgc taatcattac taaaaaattc ataaaacaga atcattccag
      841 tgaagaagat tgctatcaca gcatacaaaa aactggtcct ctcgaaccag tcttagtatc
      901 gtcgaatgaa ttaactttca gacaaggaac tgaggtgcaa gcaatactag agtacggcaa
      961 gccgagagtg acgatgtatc aaagttagtt caattgtcca taacattcta tgtataccta
     1021 tctattcagt caattcatcc gcaatcttat tgatttttcg caaacgatgg ttgacaccac
     1081 ttttagtaat cggtaccgtt agactatccg ccaattgctg aatggaatag tctggatgct
     1141 gaattcgcaa ctgggcaatc tcctgcaagt cacctgacaa ctgatctaga ccgatagtat
     1201 ccataatttt gataatatta ttgattgttt tcatactagc attgaccgtc tttgcaatat
     1261 tagcggcttc cgcatttgta gctcgattta ggtcattgcg agcctcacga agcaatttaa
     1321 cattctcaaa ttctgtcttt gtctcttctg ctccaatcac caaaagaaaa tccataatgt
     1381 cctctgcacg ttgcagatag gtaatggttc ccttacttcg ctcaatgact ttggcatcca
     1441 aaagaaattt ctgcatcaga ttggccaggt cattggcatg gtcactatag acagaagcta
     1501 tttcaagctg gtattttcct ttttcaggat ccttaacaga gcctgccgct aaaaatgctc
     1561 cacgaagata ggcttgactc cacgaatcat tttccaaaac caagggagaa atccccgtct
     1621 ctaatccaaa gaagctatca gccaaatgca aatcgttcaa tatctcattc acgccgtctt
     1681 ctatcaaaac tgcatacaca cgattctttt tgagattagg tttttgatga tggcgaatct
     1741 cagccttaat ctggtagaaa tggagtaaca gttcataaat gtgacgggca atctttgcat
     1801 tttccgtgct gatagaaaga gtcaaacctg atgaagctaa gcccaaacta cctgataatt
     1861 tgatcatagc agatagctcg ctcttatttt ggctagactg aagcagcaat tcttccttta
     1921 cttgtactgt aaaactcatg gccgcacctg caaaatcttg agcaactctt ctaccaccaa
     1981 gtcaccatca tggaaagctc cgccattttc caaacgaaga aaattcgaag aaatcacacg
     2041 attagcctgt ttttgtaaac ctgcaaaatc gtgttttacc tgtaccaagt attcatcaaa
     2101 ctggttgcta ttcatatact cttgtggaac tggctcaata ttgaccaaaa ctgtatcaat
     2161 gaattgttca gctaggtgag cattgagaac cgccacgtgg tccgcatctg agaaaaactc
     2221 agtctctccc cgttgggtca taatgttgca cacataggtc acttcagcct tggtttcttt
     2281 tagggctttg ccaatgtcag aaatcatcag attcggcaag attgaggtaa aaagagaacc
     2341 cggtccaagt acaatcatgt cactttccat aatcgtttca actacctggc gactggcttt
     2401 cggttcatca tcgttataag aatttgtcac atagacatga tcaatcatcc cattatgctt
     2461 ggaaatcttg ctctcacctg ctacctcggt cccatctgta aaaatagcat gaagggtcaa
     2521 ggcctgttcg ctagatggat aaatccgccc tgtcgtatgg aaaaagcgtg tcaacaacct
     2581 catcgcattg taggttgaac cctgcatttc tgaaatacca gcaatgatga gattccctag
     2641 tggatgacca gccaagggtc catctgaatc cgcaaaacga tactggaaaa tttgttcata
     2701 aagtttgggc atatccgaca tagccaaaag gacgttacgc aaatcacctg gaggggtcac
     2761 ctgcaaagcc tgacgaattt cccccgaaga tcctccgtca tcagctaccg tcacgatagc
     2821 ggtaatctct acatccttat cccgcaaact cttcaaaatg actggaatac cagttccgcc
     2881 cccgataact gtaatttttg gttttctcat gagcggttga ccgtctcctt ccgtctgtcc
     2941 ttatcacgat gactacgatt aacattccaa tttttctcta aatcgtctgc caaacgttta
     3001 gcaaaggcta cgcttctatg ctgaccaccc gtacatccaa cagcaatcgt tagaattgat
     3061 tttccttcct tctgataacc aggaagaatc ggttctatca agccgagcaa atgactataa
     3121 aactcttccg attcttgatg ctccatgaca tagtcaaaga caggagcatc tagaccggtc
     3181 agattacgca actccaactt gtagtaggga tttggtaaga accgaacgtc aaaaaccaag
     3241 tctgcatcca gaggtagtcc atatttgaaa ccaaaggaca tcacttctat gcgaaacgac
     3301 ggctgattat cttgactagc aaactgctcc gagattgcct tacggagatt acgaggagtc
     3361 agctctgtcg tgtcaataat attttgactc atatttttca agggagccaa aagttcacgc
     3421 tctagctgaa tcccgtccaa aacacgacca tcagccgcca aaggatggga acgacgagtt
     3481 tctttatagc gagcaaccaa ttcattatcc gtcgcatcca agaataggac tttaacatct
     3541 aaatcctctg ccccctcaat ctcatccaac acctctcgaa tctccgagaa gaaggagcga
     3601 ctccgcatat ctacaacaag agcaatctta ttgttatcct ggctgtggcg aatcaattct
     3661 aaaaattttg gcaagagagt cggtggcata ttatcaatgg taaaatagcc caagtcctca
     3721 aaagactgaa tggctaccgt cttaccagct cccgacatcc ctgtcacaat tactaaatgg
     3781 agtttgtccg acatgacatt ccctcctttt ctcttctaat caataactgc aataacttca
     3841 atctcaacct taacatcacg tggcaaacga gcaacttcca ccgctgaacg agctggaaaa
     3901 tctgctgaga aagctgtttt ataaacttta ttaaaggcta caaaatcatt catatctttt
     3961 aggaaacaag tcgtcttcac cacatggtcg aagtctgttc ctgcttctga taaaattgct
     4021 gcgatatttt tcaagacttg ctcagtctgc tcctgaatgg tttcaccaac cacttcacca
     4081 gtttcaggtg acaaaggaac ttgaccagag gcaaatagga aatttccaac aaccttccct
     4141 tgaacgtatg ggccaattgc tgcaggtgcc ttatctgtgt gaatggtttt catatctcta
     4201 tctcctttta attttttata tctattatag cacaagttct gtataaaaca gctcacctac
     4261 tactatccta cacctatttt cttttataaa aaaggaattg agatagtatc agcaacatga
     4321 aataaggcaa tccataatct acttctaaaa ctacctgctt atacccaaaa actagcctat
     4381 tcagccagtt ttcttgtttt tacatgaaac ctccaagtca gaaaaatcgc tcgcaagagc
     4441 aggtcaatca aaatcgacag ccagacgccg gcaattccta aaccgaacat ctgacctaag
     4501 acgatagcac ctacgacgcg cagcccccac atccctatca ctgtggagta gagtggcgac
     4561 ttggtatccc ccaagccctg cagagcgcca gccatgttca aagaaaccgc aaggcctggc
     4621 tggttgaaag catcgatccg taaggctgtg acgacttgat ggactgcttc taggtctttg
     4681 gtaaatagca gagcaaaact tggagctccg aaaaataaga tggtaccgag caggaacatg
     4741 atggcaacac cataggcaga gctgagaaat gcgacacgtc taactgtgag gatatccccc
     4801 tttaccaagg cttgaccgat caaaaccgcc gccgctgtcg caagaccata ggctggcata
     4861 taggtaaaac tctcgatatt gcctgcaatc atgtgagaag catagacagt cgtccccagc
     4921 cccacaatca aactgaaata gaccacctgt cccaaccgca taaccaagcg ttcggctgct
     4981 gcaggtaagg tcaaatccac catttcctta ggacttccca agtggaacaa ttgtttgaga
     5041 tcaactgcca agtcagtctg ctgtaccttt cggtaaagca agacagtacc aagcaaacga
     5101 gctaagacgg ttcccaaggc tgtccctaca atccccaaac ctgaccaaga gccaatacca
     5161 aaaatcaaga aatagtccaa gaccacatta aaaccattgg tcagtaagct cattttcata
     5221 ggggatactg tatcaccaga tgcccgcagg attgttccca aaatagtcat caaggcctga
     5281 aaaatcgtca aaccaccaac ccagtaaaaa aatgcctggg caccagccaa actctccgca
     5341 tctgctccca ttaagaccag catctgccgc ccgaaaacaa gggatagtaa accaaatagc
     5401 acacctacac ctactgaaag cactaaggct tgacggacat gaaaggtaag agattcccta
     5461 tctcctgcgc caatagaccg agcaatcaaa gctgttgccc caactcccaa agccatatag
     5521 accgccaaat agacgttcaa aatcgtattg gcaagaccaa ctgccgtcac cgctaccagc
     5581 cccagctgtg caatcaagag ggtatccaca aaaccaacta aggtctgaaa gatattttcc
     5641 accgtagcag gcaaggacaa acgaataatt tcttttccta aactttcttg cttcattttc
     5701 ctctccacga ttttatttag aataattata aataaaactc ggtgaaaaag aaagaaaaca
     5761 gggagtgggg aagaactcga taagtcaaaa aggagttcat ctctccaccc ccgcatagct
     5821 ccaaagtttc agggaacctt tggaggttag agataaggcg aacaaagttc gcatcagtcg
     5881 tataggtcag atttggagtg gaaaacacga attgctgaga tttgcttcgc aaaccttatc
     5941 tccaaccttt ttacctagct tttctgcttc aaggctcggg ctgaaacagt tcccagaact
     6001 gcttcactcc actggactgt taaagccaat caaccactgc gctgagatgt tgacacgaac
     6061 tctgagaagt acaccgatac cgagcctagt tacctagaac ttcatttttt atagatttct
     6121 ttccggtgcc cgatttcaag ggccaggatg accattttgt tatcaataat atcacaaatt
     6181 acacggtaat tccccactct ataccgccac agccccttat agtctcctac caaggccttc
     6241 ccaaattgtc ttggattctc caaaccgtcc aaacgttttt tcaaatcctt agcaagcatc
     6301 atccccacat gcctgtccat ttttttcaac tgcttcaaag catcatcgct gagaacaagc
     6361 ttataagcca tcttctaact ccgcaagaac atcatcaatc gagtgtgtaa caggattttc
     6421 ttggaatttc ttcaaagcct tgataccaat ttcatagtcc aactggtctt ctatctgttc
     6481 agcaagtgca gttttgaata gctcagacag gctttttcct gtatgaatag catagcttct
     6541 gaaaatttct tcttctgcat cgtttaatcg caaggataca attcccatca ttccacctac
     6601 tttcttcttg taatacattg taaaacaatt cgttcaaaat gtcaattgac accagaaaaa
     6661 cagaccacgt ttgtagtctg tcagtgaata tttatttcgc agtctttcag tttactttta
     6721 gtactaggca acgagctgca gactgtacta aggtacggca aaacgagttc aaataatgat
     6781 gagattattt gaacttggaa ataaggaaac ggagtttcct catacgtcga cgtaataaag
     6841 gaaaaactga acgactgtat tacggatgca agcggtgcaa catacgtggg aacggaatgg
     6901 cttcacggat gtgttttgta cctgcaacaa aagtcaccat acgctcgata ccgataccga
     6961 aaccaccgtg tggtactgaa ccgtacttac gtaggtcaag gtagaattcg tattctgact
     7021 tatccatgcc gagctcgtcc atcttggcta ccaaggcatc gtaattgtcc tcacgcatag
     7081 aaccaccgat gatttcaccg tagccttctg gtgccaagag gtccgcacaa agcacgcgct
     7141 ctggattacc tggaactggc ttcatgtaga aggccttgaa gcttgctgga tagttaacaa
     7201 cgaaagtcgg tacaccaaag tagtttgaaa tccaagtttc atgtggcgat ccaaagtcat
     7261 caccatgctc gatgtgttcg tagtctgtat cttcatcagc ttcatgctct tgaagaaggg
     7321 taatggcatc atcataagcc acacgtttga atggctccgc aatgtagcgt ttgagagcat
     7381 ccacatcacg ctcaagagtt tccaaggctt gtggagcacg gtcgataaca ccttgaatca
     7441 aggctttgac ataagcttct tgcaaatcaa gtgactcttc atggctgagg aatgaatact
     7501 cggcatccat catccagaac tccgtcaagt gacggcgagt ttttgatttt tccgcacgga
     7561 aaacaggtcc gaagtcgaaa acacgaccaa gggccatggc accagcttcc aggtacagct
     7621 gaccagattg gctgaggtag gcaggttgac caaagtagtc agtttcgaac aactctgtcg
     7681 aatcttctgc agcgtttcca gacaagattg ggctatcaaa cttgataaag ccattctttt
     7741 caaagaattc ataagtcgca tagatgatgg cgttacggat ttgctggata gcgacctgtt
     7801 tacgagaacg caaccagagg tgacggttgt ccataaggaa gtccgtaccg tgttcttttg
     7861 gtgtgattgg gtaatcttgt gactcaccga ttacttcaag gtctgtcaca tccaactcat
     7921 aaccaaactt agaacgctca tcttccttga caattcctgt cacatagaca gacgtttctt
     7981 ggctgaggcg ttttgctaca tcgaacttct cagtcccagc ttcttcaccg aatttttcga
     8041 tgaagtttgg ttttaaggca actgcttgga agaaggctgt tccgtcacgc aattgcagga
     8101 aggctaactt gcccttgcct gatttgttgg caacccaggc accaatagtc acttcttgac
     8161 cgacataatc tttgacttgg ttaatggtaa tcaattttct agacattatt tttcctcttt
     8221 ttattttttc ataaatgtat gtagtcgagc cactgcttct ttgagcgtgt ccatatctgt
     8281 cgcatagctg aggcggatat tctcaggagc tccaaaacca gcacctgtca ccattgcaac
     8341 tcccacttct tccaaaatgg ctgtggtaaa ttcagttaca tctgtgtagc ctttcatttc
     8401 catagctttt ttaacattcg ggaagagata gaaggcccct tctggcttaa tcacttcaaa
     8461 acctggtact tctgccagca agggataaat ggtattgagt cgctcttcaa aggcctgacg
     8521 catggtttct actgtatctt gcggacctgt aaaggcttca atggccgcat actgagcagc
     8581 agtcgttaaa ttcgaagtcg tttgtccgac aatcttactc atggccgcaa tgatttcagg
     8641 attaccaacg gcaaaaccaa cccgccaacc tgtcatagca taggcctttg ccaccccatt
     8701 gacaacaata gtctgctgac gaatgctttc tgaaatgctt gaaatcggtg tgaagccatt
     8761 tccattatag accaaacgac cataaatatc gtctgccaag atgagaatat cgtgctctac
     8821 agcccagttt ccaattgctt ccaattcctc acgactgtaa atcatcccag tcggattaga
     8881 aggagtattg agtaggaata ctttggtctt atctgtccga gctgcttcca gctggtcaac
     8941 cgttaccttg aaattatgct cttcccttgc agtgacaaag acaggcaccc catcattcat
     9001 cttgatttga tctgcataag aaacccagta aggtgtcgga ataatcacct catcaccagg
     9061 attgatgacc gccgcaaaga aagcataaag aacaaactta gcacctgtcg caagaacgac
     9121 ttgattgcgt tcgatagagt agccatagaa attctcaaag taggtattga ctgcgtcctt
     9181 caactcagga tgccctgaag ctaccgtata aaaactcgcc ttaccatctt cgatagactt
     9241 aatagcagct tcttgaatat ttttcggtgt gacgaagtct ggctcaccca aggtcagctc
     9301 taaaatatca cgtccttccg ccttcaaggc ttttgcacgc gcacctgctg ccagagttac
     9361 actttcttcc atctctaaga ctcgtcttga taacttgctc ataagccctc ccgtttgata
     9421 aagttccctg tttcaaattc aactaaatag taggcagtac ctgacttgac ttcccaaata
     9481 ggtttgccat cccttaaacc tagaatagtt cgctctactt ggcttgcacc gtttttcttg
     9541 gcaatagctt gcgcttcttc ttgcgaaatt ccctgaacta aaggatagac atatactgtg
     9601 ctagaagcct ctggaatcaa aactgcaacc tcttctccct gactggtgac accaaataca
     9661 caaaaatagg tttcagtacc attgtagata gaaaaatcat tgacagtttg caaatcagca
     9721 tattctgtag ctactgcaac tgcatgacta cgcacattgt tgaaaggggc agcagcaata
     9781 tcccaaatag taaaaattga aaaagtcaca acactgagaa ggataaaaga gcccaacact
     9841 aactgaccag tcttggaagc catccactcc aacagttgaa aaatcttttt ttccatagta
     9901 gtcccattat actacaaatt aaagcatatt tccattcttt ccgtgattgt aaataaaatt
     9961 tccttttcct tcacacaaag aaaaggatat aaaaacctac attcacagtt ttgcacttcc
    10021 atctaagact atttctcaaa tgctcattgc tcgttttttt ccagtacagt tagtattacc
    10081 tcaaaaaacg aacttgatta actaaaaact atcttttcca taaaagtact ttacctatga
    10141 atataggatt taactttttt tgcttgataa ccgaacgatt tattgctaaa atattctcat
    10201 gagaactgtt gaacaatctt taaacgagaa caagcatgca cttcacagtc tagtcgtctt
    10261 tcgcagagca gctaatacta tcgcaaaatc agaattagaa acaatcaaaa aatacggact
    10321 gacagtttgt cagtttggag tgatggaggc actctacaac aaaggaaatc tgcggattca
    10381 agacttaatt gataaattat taagcacttc tggcaacatg actgtcgtta taaagaacat
    10441 gattcgagat ggttatatct acaagactat cgatacaaat gaccgtcgtg cttccttgat
    10501 tgctctcact ccacttggac gcgaaaccat tgaaaaaatc cttccagagc attatgatca
    10561 cgttggagag attttttctg tccttagttc agaagaacaa gaccaattag ccgagatact
    10621 taaaaaattc aagaatcagc agacctagga ttgtcaacaa aagacaaata cgatatacta
    10681 aatttatgtt tttatgaaaa ggaggccatc atggcaaacg tattgttttt agttggttca
    10741 ctacgtgacg gttcttttaa ccatcaaatg gcaaaaaaag ctgaagcact cttgactgat
    10801 aaggctcaag tatcttacat tgatcttgca aaaattcccc tattcaacca agacatcgag
    10861 acacctatcc ttcctgaaat tgcagagcta cgtgcacaag tagacgctgc ggatgctatc
    10921 tggattttct caccagtata taactatgca atcccaggca tcgtaaaaaa tgctctcgat
    10981 tggctcagtc gttcgctcga tttatccaat ccaaaaggac catctatcct acaagataaa
    11041 atcacaactg tctctgcagt cgcaaacagt ggtcacgacc acctattcaa agacttccaa
    11101 caccttcttc catttattcg cactcagatt gctggtgaat ttacaggttc aaccatcaat
    11161 ccagaagcat ggggaacagg tgtattggag ttatctgacg aaactctaac aaaactcgaa
    11221 caacaagtag ctgacctgtt ggcagcaatt caataataat caaaaaaacc agaatttctg
    11281 gtttttttga ttaacatttt aagagcattc cgacttctgc caagaataat tactactttg
    11341 atgacttctc aaaatcattc cccaacttca tttctcacac actgtaaggc agctccgatg
    11401 acttggtgca tatcgtagta acgataatga ccaagacgcc caccgaatat gacattctct
    11461 tgttgttctg acaatttctt atatactgtg tatagatagt tattgcgatt attgttgact
    11521 ggatagtatg gctcatcgcc tcgtttccag gtcttcgaat actcacgtgt aataatggtt
    11581 ttcggttgtg taccaaattc gaagtgtttg tgttcgatga ttcgagtata tggtgtctcg
    11641 ctatctgtat agttgacaac cgcattccct tgatagtttt ccatatccaa cacttctgtc
    11701 tcaaatcgaa ggctacggta ctccaattca ccaagttggt aatcaaagaa ctcgtcaatc
    11761 attcctgtaa agacaatctt tggataggta gctaaatagt cttccttatt ggcaaagaaa
    11821 tcaacatttg tctccacatc gatattttca tgttccaaca ttttttctac aatctgcgtg
    11881 taaccgccga ttggaatacc ttgataagta tcgttaaagt agttattatc ataagtcaaa
    11941 cgcacgggca aacgacggat gatgaaggct ggtaactccg tacatggttt ttcccattgt
    12001 ttttctgtgt aggacttgat caatttttca tagatgtctg taccaacaag tgagatggct
    12061 tgctcttcca agttttcagg cgttttacca cccaacacag ctcgttgctc tgcaatcttc
    12121 gcttctgctt cagcaggtgt caccacgccc cacagtttat tgaaagtatt catgttgaag
    12181 ggaagattgt aaatctcacc tttgtagttg gcgactggtg tgtttgtata gctgttgaac
    12241 tccgcaaact gattcacata atcccaaatt tctttttcag aagtatggaa aatatgtgcg
    12301 ccatattcat ggacttgaat accttctact tcttttgtat agatgttacc cgcaatgtgg
    12361 tctcgttttt cgataacctt tattttttta ccctttttag
//
LOCUS       DASFSW010000058        12193 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.058, whole genome shotgun sequence.
ACCESSION   DASFSW010000058 DASFSW010000000
VERSION     DASFSW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.058"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            90..950
                     /locus_tag="U1099_001166"
     CDS             90..950
                     /locus_tag="U1099_001166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867492.1"
                     /translation="MKKTIWNASLEESMNLVTDKYIKTSMDDVNQYGHKKKILGFFTM
                     VLFIGLLSLIGSYIDKESGVLLFLKAELLFRIPLVLSLLFLFCYLMELRKVKEYRVLS
                     YYIFNRYMFLVMSCFILQFWLICAVGFAILWGSVLSIIVNVGIFSIVVSERLISFVKK
                     TEGVLYQGQPSNNILYKFLEKFVAFSKRYGGGLVVLLLVSRFVFKNSFQSASGEGIYH
                     NDFLRSLAMTFSVFFPLLPFYFSVAIATSCIEGYYLEKYSEDYRQLSGYSVEDWYGKK
                     SRRYKESLGK"
     gene            complement(1163..2032)
                     /locus_tag="U1099_001167"
     CDS             complement(1163..2032)
                     /locus_tag="U1099_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM4867493.1"
                     /translation="MKIDIRIPQAFPQLTVKEVLEDYFLIPRKIRHFLRTKKHVRVNG
                     ELINWQSPIAAGDLLELTFDQEDYPEKSIPLGQANLVEELYQDEHLIIVNKPEGMKTH
                     GNDPTEIALLNHVSAYVGQTCYVVHRLDMETSGAILFAKNPFILPILNRLLEDKVIYR
                     DYLALCQGQLRKTDWTITDKIGRDRHDRRKRVVDNRKGQVALTQVRLLKTLSKNSLVT
                     CRLQTGRTHQIRVHLAHHDHALIGDPLYSQVAAPRLMLHAQKLSLTHPLTLEEIRVEA
                     RSESFEKVLKNMK"
     gene            complement(2034..3002)
                     /locus_tag="U1099_001168"
     CDS             complement(2034..3002)
                     /locus_tag="U1099_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM4867494.1"
                     /translation="MTQVYDITIIGGGPVGLFAAFYAHLRQAKVKIIDSLPQLGGQPA
                     ILYPEKAILDIPAFPSLTGQELTDNLLAQLAPFDTTICLNETLTAIEPGEVISLTTNK
                     GSHQTKTLIIAMGGGAFKPRPLEIDGADSFDNVHYHVSNIQQYADKDIVVLGGGDSAV
                     DWSLAFEKIAKTTQIVHRRDNFRALEHSVEELKQSSVTIHTPFIPKGLSGENGRASAI
                     DFDKVKSEDKLTLSFDHLFVNYGFKSSVGTLKEWGLELNRHRIVVNSKQETSVPGIYA
                     IGDCCFYEGKVDLIATGLGEAPTAVNNAMNYLNPNEKVQPKHSTSL"
     gene            3155..5368
                     /locus_tag="U1099_001169"
     CDS             3155..5368
                     /locus_tag="U1099_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM4867495.1"
                     /translation="MDDLQHQPIEVPEDFQIDHEDRPSRRRRGNRGSVDKPKKKSRIP
                     EPIRKFWRRYQLTKILIILMVLTVLTVGGYLFFLAKTANVGDLQQALKATTIIYDKDG
                     AEAGTLSGQKGTYVELDAVSDNLENAVIATEDRSFYKNSGINYQRTILAALTLGRSGG
                     GSTITQQLAKNAFLTQDQTISRKAREFFLALEINKKYSKQEILTMYLNNAYFGNGVWG
                     VEDASQKYFGIPASDLTLEQAAVIAGMLKGPEIYNPYYSLENAVNRRDTVLQNMVNAG
                     YIDQASADAGFQVDLASQLSDTYTGKQSNYSYPSYFDAVIAEAIERYGLKESDIINNG
                     YRIYTEMDQNSQASMQVIFNDETMFPTSSFDGTHAQAASVALDPRTGGVRALVGRVNS
                     SEDAVFRTFNFATQAKRSPGSTIKPLVAYAPAVAAGWSIDMALDNHTETYGDYTLHNY
                     DYSTSDTIPMYQALALSYNLPVAYIVNTLGIDKAFEYGQKFGLDMENVEKVLGVSIGS
                     GVETNPLQMAQAYATFANKGVMKDAHLITRIETASGKVLAEHRETSTKVIDRSVADKM
                     TAMMLGTYTNGTGVTADTANYHIAGKTGTTETSFDVNLVNDQWHIAYTPDLVISQWVG
                     FEQTDENHYIDSSSYWKAPSVFQTVTNSILPYTAGTKFEVENAYAQNGIVEVAPDEAA
                     QTESSLGQEQVQEISEQAKNLIEQTKQNLIDAKLPERAKTLWDNITSWFGELSNP"
     gene            5402..5554
                     /gene="rpmG"
                     /locus_tag="U1099_001170"
     CDS             5402..5554
                     /gene="rpmG"
                     /locus_tag="U1099_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM4867496.1"
                     /translation="MALKKASLACTVCGSRNYSISLSSNPKPTRLEVNKFCKHCGQYT
                     VHKETR"
     gene            5564..5740
                     /gene="secE"
                     /locus_tag="U1099_001171"
     CDS             5564..5740
                     /gene="secE"
                     /locus_tag="U1099_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEM4867497.1"
                     /translation="MKFIKDIIRILKDTSWPTRKQSWTDFVSVVEYTAFFVAVVYLFD
                     LILSRGIMSLINLF"
     gene            5850..6389
                     /gene="nusG"
                     /locus_tag="U1099_001172"
     CDS             5850..6389
                     /gene="nusG"
                     /locus_tag="U1099_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEM4867498.1"
                     /translation="MYDSFDKGWFVLQTYSGYENKVKENLLQRAHTYNMLENILRVEI
                     PTQTVQIEKNGEVKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRQILVSMGQTVQEFDIDVKVGDTVRIIDGAFTDYTGKITEIDNNKVKMVI
                     SMFGNDTIAEVNLSQIAEL"
     gene            6533..11611
                     /locus_tag="U1099_001173"
     CDS             6533..11611
                     /locus_tag="U1099_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEM4867499.1"
                     /translation="MKQKWSQIENKQRFSIKKLSVGVASVSIGFFITGVPMVQADTSG
                     EGLESTVAVATDMDSRQNSAVEKKEDGPLSDEPVKTEQVDEPVAEEGVVEEVVDTEAG
                     EESGLLTDQAATEIKTTAGKTTDESKEKEDISGKEASAPQTIPQESQLEPEEVTRGRY
                     ILQFSEENRNLVLDKLKKIDGVKIVHEYKEVLTGASVEVGKESLSDVKAITELTSLEE
                     SRRIRPTLHTAKQLVGALKASSKYQTDGRGMVIAVIDSGLDIKHKDMRLDDGVIPKIK
                     DITPSTTGTYTLKVPHGYNYVSGNDNLYDDTHEPHGMHIAGTLAGNATDEEVASKKGV
                     DGIAPNAQLLVYKIFSNDPKNYKAETEDAAYAAIEDAIKHGADVISLSVGYYDSGLPG
                     NAYYTIAKRAAEKGIIITAAIGNAGASSSDTSFDLHTNNALGAVDTATTVGVAATPAV
                     IAVGSARNTHLVQREFMLNGQSFGYYPIGYTTLTEGKYEFVDAGNGHWEEVQGLDLAG
                     KVAVIKKDKFDLKDAVRNLKFKDVAGIIVINTDQGWNKDYYRTHQLLVDDKTLLSYSS
                     IWGISLSGEDGRRLLEVANQSQGNTGLVLKPTIGMKKLIEVPTVSGFSSWGPTVNLEL
                     KPEIVAPGEDVYATLNDNRYGSMSGTSMASPIVAGASALLLPRIRQMTPPEGMTRMDL
                     LRIILMNTATPLVDVLDSSGHALENSPRQQGAGLLQIDRAFETDVILHHRLKGGVELK
                     EIGRETEFEVTLENLGNQQRSFAISAGKVLTSQDVPVDRIGRSGKVVKEIHATEIKGS
                     SIHLSEQSIQLGPKEKRTIRLKLDAGEAKDQFAEGYIYFKSLTDGQSDISIPYFGFVG
                     DWSKERIVDAPAWETSSKLKLTSVLSSYKHNKSGRYIELGREKIQDNQSPLNPDNIAI
                     QNQHSDSQIGNAFVRFALLRDITNYDLDIVKEATEDAPVLRRIDTGTMLSRVRYVDYF
                     ESLSEYFKLRTPIELHRWDGKVYDASKDENIPAPEGQYFFRLRVKNKENGAYQYTYLP
                     VKIDNQKPEIVAIDTNRLTSHRELVVTAKDNNKVWEVRANLNGEDLLVEKVVDDAGQL
                     HYHLKEVELPLDAKNHLRVEVMDIAGNVVAVEKDLVAPVIQFKNLEDLMAIRSKKTVE
                     IKANVSAQVSNVQANLDAQAVNYSLENGQLSLQIPEQSDGRHSFELILKDKDGKLIYT
                     KTLNYLVDNEKPTIDLDIEKDEEDEEVIQIGKNGRFTLKGKVSDNVSLPKNIKLYYSN
                     LDIGKGELKIIDVKEDGSFEQDFFKSDFPRAIMLTAVDEKGNKLKDLRINTSPESLDE
                     EEETEVPITVNNWLIDPIRFNKESLGRELDSGLVDFKKQEDGTYLFTFEIEAETDQAH
                     SVRINGGEKRYFEDGKLTYPVTLIEEGNVVDISVYNEADELTYTKKYQMLVDTENPVL
                     QLENEVLPLERQVVDSEEDEDEENQYAGVLLADADGHLTLTGSAKDNGIYWSLKINED
                     FVARGGFWRQYGNNEKAFRYELHSLKDGDTVKLDLSDSFGNALVKKYKVRLNDKEVSE
                     QVPEKDLHVEQSDKDQAPSIPVPKSEAHVPMPKEENSLAPQTESTEIALLTGDTREDG
                     VEHLVRFTKHEEALGISDERIEVSVPHREFFERSGRGETGALAADTSGKLPQTGDSLG
                     SVFISALLGLFGGAMVLGNLKRKE"
     gene            complement(11654..11724)
                     /locus_tag="U1099_001174"
     tRNA            complement(11654..11724)
                     /locus_tag="U1099_001174"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11690..11692),aa:Cys,seq:gca)
BASE COUNT         3695 a         2141 c         2951 g         3406 t
ORIGIN      
        1 ataaatacaa gcaggaaata ttaaaattag tagcaaaaag tgtatttaac taagagctaa
       61 gactatttgc tattggttaa ggagaaaaca tgaaaaaaac aatatggaat gcttctttag
      121 aagagagtat gaatcttgta acagataaat acatcaaaac ttctatggat gatgtgaatc
      181 aatatggaca taagaagaaa attttaggtt tttttacgat ggttcttttc atcgggctac
      241 tcagtttaat tggaagctat attgataagg aatctggagt attgttgttt ttaaaagctg
      301 aactattatt tcgaatccca ttagtactaa gtttgttgtt tttattctgt tatctgatgg
      361 aattgagaaa agtcaaggag tatagagttc tatcctacta tatttttaat aggtatatgt
      421 ttttggtgat gagttgtttt attttacaat tttggctgat atgtgctgta ggatttgcga
      481 ttctatgggg ttcagttctg tcaattattg tcaatgtcgg tatttttagt atagtggtat
      541 cagaacgtct tatttcgttt gtaaagaaaa cagaaggagt attgtatcaa ggacaaccat
      601 caaataacat cttgtacaaa ttcctggaaa aatttgttgc tttttcaaaa agatatggtg
      661 gtggtttagt tgttcttctc cttgttagtc gttttgtatt taaaaatagc tttcaaagtg
      721 caagtggaga aggcatttac cataatgatt ttttacgctc cctggcgatg acatttagtg
      781 tttttttccc cctcctcccg ttttatttct ctgtagctat tgctactagt tgtatagagg
      841 gatattactt ggagaaatat tccgaagatt accgtcaact atctggttat tccgtagaag
      901 actggtatgg taaaaagtct aggcgatata aggaaagttt gggaaagtag gttttgttta
      961 gatcatatta gtttactttt ggtttatggc taagcaatgt attccaaaat cccaagttgc
     1021 ataatttttt gatgcttgag ctattaactt gtaaacccag acaaaattac cttgtctgga
     1081 ttttttggtt agtggaagca taaaaaaatt cactggatta gtccagtgaa atagggatat
     1141 agcactctaa gggtgcagct atttatttca tgtttttcaa gactttctca aaactctccg
     1201 accgtgcttc cacgcggatt tcttccaagg tcagcgggtg ggtcaggctg agtttttggg
     1261 cgtggagcat aaggcgaggt gctgcgactt gactgtagag cggatcacca atcagggcgt
     1321 gatcgtggtg ggctagatgg acccggattt gatgggttcg gcctgtctga aggcggcagg
     1381 ttactagact atttttgcta agagtcttca agaggcggac ctgggtcagt gccacctgtc
     1441 ccttgcggtt atcgaccacc cgctttctgc ggtcgtggcg gtcacggccg attttatcgg
     1501 taatggtcca gtctgtcttt cttagttggc cttggcagag agctagatag tcacgataga
     1561 tgaccttgtc ttccaagagc cgattgagaa tgggcaggat aaagggattt tttgcaaaga
     1621 gaatggctcc gctagtttcc atatcaagcc tgtggactac atagcaggtc tggccgacat
     1681 aggcagagac atggttgagc agggcaattt ccgtcggatc attgccgtgg gttttcatgc
     1741 cttctggctt gttgacgata atcaaatgct catcctgata gagttcctcc accaagttcg
     1801 cctgtcctag aggaatgctt ttttcaggat aatcttcctg atcaaaggtc aattctagca
     1861 ggtctcccgc agcaatcggg ctctgccagt taatcagctc accgttgaca cggacgtgct
     1921 tcttggtgcg gaggaagtgg cggatttttc tgggaatgag aaaataatct tccaagacct
     1981 ctttgacagt cagttgtgga aaggcttggg gaatacgaat gtcaattttc atcttatagg
     2041 ctggtcgagt gctttggttg cactttttcg tttggattga ggtagttcat ggcattgtta
     2101 acagcggttg gggcttcgcc cagtccggtc gcaatcaggt caactttgcc ttcatagaaa
     2161 caacagtcac cgatagcata gatgccaggg acagaggttt cttgcttgct gttgaccacg
     2221 atgcggtggc gattgagctc caatccccat tctttcagcg tgccgacaga tgatttgaaa
     2281 ccgtagttga caaagaggtg gtcaaaagac agggtcagct tgtcctcgct ttttacctta
     2341 tcgaaatcaa tggcagaagc tctgccattt tccccagaaa gccctttagg gataaaaggt
     2401 gtgtgaatag taacgctgga ctgcttgagc tcctccacgc tgtgctcgag ggctcggaag
     2461 ttatcgcgtc ggtggacaat ctgggtggtc ttggcgattt tttcaaaggc cagggaccaa
     2521 tcgaccgcag agtcaccacc acccaatacg acgatgtcct tgtcggcata ctgctggata
     2581 ttagacacgt ggtagtggac gttgtcaaag ctgtcagcac catcaatctc cagcggacgt
     2641 ggcttgaagg ccccgccacc catggcgata atcagggtct tggtttggtg actgccttta
     2701 ttggtcgtca ggctaatgac ctccccaggc tcaatggcag tcagggtttc attgaggcag
     2761 atggtggtgt caaatggagc cagctgggca aggaggttgt cggtcaattc ctgtccggtt
     2821 aggcttggaa aggctggaat gtccaaaatg gccttctcag gatagagaat ggcaggctgg
     2881 ccacccagtt ggggaaggga gtcaatgatt ttgaccttgg cttgacggag atgagcgtag
     2941 aaggcggcga agagaccgac tggtccgcca ccgatgatag taatgtcata tacttgtgtc
     3001 atgggaatcc tcttctttgt tcagaatgac cctattttat catatttttg gaaaaaattc
     3061 cctgtctgtt gtcacaagtt atcgcaggct tttttctgct agtttttagg aaatggtgta
     3121 taatggtaca aaattcacta gaaagaggta aagtatggac gatttgcaac atcagccgat
     3181 tgaagttcca gaagatttcc aaattgatca tgaagaccga cctagccgtc gccgcagggg
     3241 aaatcgtggc tctgttgata aaccaaagaa aaaatcacgt attccggagc cgattcgtaa
     3301 attttggcgc cgttatcaac tgactaaaat tttaatcatt ttaatggtcc tgacagttct
     3361 tacagtaggt ggctatctat ttttccttgc aaagacagct aatgttggtg atttacagca
     3421 ggcgctaaag gctacaacga ttatttacga taaagatggt gcggaagctg ggacattgtc
     3481 tggtcagaag gggacctatg tagagctgga tgctgtctct gataatttag agaatgccgt
     3541 tatcgctaca gaagatcgga gcttctacaa aaattcaggc attaattacc agcgtacaat
     3601 tttagccgcc ttgacgttag gtcggtctgg gggtggttca actattaccc agcaattggc
     3661 aaaaaatgcc tttctaactc aggatcagac catcagtcga aaggctcgag aattcttttt
     3721 ggctctggaa atcaataaaa agtacagcaa gcaagaaatt ttgaccatgt atctcaacaa
     3781 tgcctacttt ggaaacgggg tctggggagt ggaagatgca agccaaaagt acttcggtat
     3841 tccagccagt gatcttactc ttgagcaagc ggccgtcatt gcaggtatgc ttaagggacc
     3901 tgagatttat aatccttatt attctctaga aaatgcagtt aatcgtcggg atacagttct
     3961 gcaaaatatg gtcaatgctg gttatattga tcaggcgtct gcagatgcag gtttccaagt
     4021 agacctagcc agtcagcttt cagataccta tacagggaaa caaagcaatt attcctatcc
     4081 atcctatttt gatgctgtga ttgctgaggc gattgagcga tacggactga aagaatcaga
     4141 cattattaat aacggctata ggatttacac agagatggat caaaattctc aagccagcat
     4201 gcaggtgatt tttaatgatg agactatgtt cccaacatca agttttgatg gtacccatgc
     4261 tcaggctgcc agcgtagctc ttgatcctag gactggtgga gttcgcgcct tggttggtcg
     4321 agttaacagt tcagaggatg cggtctttcg gaccttcaac tttgcgacac aagccaagcg
     4381 cagtcctggt tcaacaatca agcctcttgt tgcctatgct ccagcagtag cggcgggctg
     4441 gtcgattgat atggctctgg ataaccatac agaaacctac ggcgattaca ccttacataa
     4501 ctatgattat tcgacttctg ataccattcc aatgtatcaa gctttggctc tctcttacaa
     4561 tttaccagta gcttatatag tgaacacctt gggtattgac aaagccttcg agtatggaca
     4621 aaagtttgga cttgatatgg aaaatgtcga gaaagttttg ggtgtttcaa ttggttccgg
     4681 agtggagacc aatcctctgc aaatggccca ggcctacgct acttttgcga ataagggtgt
     4741 gatgaaggat gcccacctca tcactcgtat tgaaacggct agtggaaagg ttttagcaga
     4801 acatcgagaa acaagtacca aggtgattga ccgctctgtc gcagataaga tgacagcaat
     4861 gatgcttggt acctatacca atggtacggg agtgacagca gatacggcca attatcatat
     4921 cgctgggaaa acaggaacaa ccgaaacgag ttttgatgtc aatctcgtca atgaccagtg
     4981 gcatattgcc tacacacctg atttggtcat cagtcagtgg gttggttttg agcagacaga
     5041 tgaaaatcat tatatagata gcagtagcta ctggaaggct ccgtcggtct tccagactgt
     5101 aaccaattca atcttgccat atactgcagg aactaagttc gaagtagaaa acgcctatgc
     5161 acaaaatggt attgtagaag tagctccaga tgaagctgcg caaactgaat caagccttgg
     5221 tcaagaacag gtacaagaaa tttcagaaca ggcgaaaaac ttgattgagc aaaccaaaca
     5281 aaatctaatc gatgccaagt tgccagaacg agcaaaaacc ttgtgggata acattacgtc
     5341 ttggtttgga gagttgtcaa atccataaaa acatggtaga atgaaaagga tggagggctt
     5401 tatggcacta aaaaaagcaa gcctagcctg tactgtttgt ggttctcgaa actattcaat
     5461 cagcctttcg agcaatccca agccaacacg gttagaagta aataaatttt gtaaacactg
     5521 tggacaatac actgttcaca aggaaaccag ataggagtca cgcatgaaat ttatcaaaga
     5581 cattatccgc attttaaaag atacgtcttg gccaactcgt aagcagagct ggacggattt
     5641 cgtgtctgta gtggaatata ctgccttttt cgtagcggtg gtttacctct ttgacttgat
     5701 tttgtcacga ggaatcatga gcttgatcaa tcttttctag aaaatacttg ccatttgtaa
     5761 aatggaagtg atataataag gaaaagcgaa agccttcggg ctttttttag cgtctaaaaa
     5821 ttccgtgttt ccaaagaaag gaagtaacaa tgtacgatag ttttgataaa ggctggtttg
     5881 ttttacagac ctactcagga tatgaaaata aagtgaagga aaacttgctc caacgtgctc
     5941 acacttataa catgttggaa aacatccttc gtgtagaaat tccaactcag actgttcaga
     6001 ttgaaaagaa cggtgaggtc aaggaagtgg aagaaaaccg tttccctggt tatgttttgg
     6061 ttgagatggt tatgaccgat gaagcttggt ttgtggttcg taatacgcca aatgttacag
     6121 gtttcgtggg ttcacacggt aaccgttcaa aaccaactcc acttttggaa gaagaaattc
     6181 gtcaaatctt ggtgtctatg ggacaaaccg ttcaagaatt tgatattgat gtcaaagttg
     6241 gcgacactgt tcgtatcatc gacggtgcct tcacagacta cacaggtaaa attacagaaa
     6301 tcgacaacaa caaggttaaa atggttatct ctatgtttgg taatgatacc attgctgaag
     6361 ttaatttgag ccagattgct gagttgtaat aagttgttaa agcgcaggga agcctgtgct
     6421 ttttcttgtt tttgagggct aattcaaaaa aaacttgcca aagatttaaa aattttatag
     6481 aattaagtat acctaactta taaattaatt aatcttaaaa aacggaggtt ttatgaaaca
     6541 gaagtggtct cagattgaga ataaacaacg ctttagtatc aagaagttaa gtgtcggtgt
     6601 ggcatcggta tcaataggat tttttatcac gggtgttccg atggttcaag cagatactag
     6661 tggagaagga ttggagtcta cagttgcggt cgcaacggat atggatagta ggcaaaattc
     6721 tgcggtagag aaaaaagaag atggaccatt atcagatgag ccagttaaaa cggagcaggt
     6781 ggatgaacca gttgctgaag agggagtggt cgaagaagtt gtagatacag aggcgggtga
     6841 agaatcaggt cttctcacag atcaagctgc gaccgagata aaaactacag ctggtaagac
     6901 aacagatgag tcaaaggaaa aagaagacat tagcggaaaa gaagctagtg ctcctcaaac
     6961 aatcccgcag gaatcacagc ttgagccaga agaggtgaca agagggcgct atattttaca
     7021 attttccgaa gagaaccgaa atcttgtatt ggataaatta aagaaaattg atggcgttaa
     7081 aattgttcat gagtataagg aggttttaac gggagcatca gtagaggtag ggaaagaaag
     7141 tttgtctgat gtgaaggcaa ttaccgaatt aacctcttta gaagagagtc gccgtatccg
     7201 accgactctt catactgcta aacagctggt tggtgcctta aaagcaagtt ctaaatatca
     7261 gacagatggt cgcggaatgg tgattgcagt cattgactcc ggtttggata ttaagcacaa
     7321 agacatgagg ttggatgatg gtgtcattcc taaaatcaag gacattactc catctacgac
     7381 aggaacatac acactgaaag ttccccatgg atataattat gtatctggta acgataatct
     7441 ctacgatgat acccacgaac cacatggtat gcacatagca ggaactctgg ctggaaatgc
     7501 aacagacgaa gaagttgcgt ctaaaaaggg agttgatggg attgctccca acgcacaact
     7561 gctagtctac aagatttttt caaatgaccc taaaaattat aaagctgaga cggaggatgc
     7621 tgcttatgct gctattgaag atgctatcaa acacggggca gatgtcatca gcttgagtgt
     7681 tggttattat gatagcgggt taccagggaa tgcttactac acgattgcta agagggcagc
     7741 agaaaaggga attatcatta cagcggctat tggtaatgct ggtgcctcct cttcagacac
     7801 ctcctttgac ctgcatacaa ataatgcctt aggagctgtg gatacggcaa caacagttgg
     7861 tgtggcagcg actcccgcag ttatcgcggt tggttcggcg agaaataccc atcttgttca
     7921 acgagaattt atgctgaatg gacagtcttt tggttactat cctattggct atacaacgct
     7981 tacagaggga aaatatgaat ttgtagatgc tggaaatggt cattgggaag aagtgcaagg
     8041 gcttgatttg gctggtaaag tagcggttat caaaaaagat aagtttgact tgaaagatgc
     8101 tgttcgtaat ttgaaattca aggatgttgc tggaattatt gtgataaata ctgatcaggg
     8161 atggaacaag gattattata ggacccatca gttgttggta gatgataaga ctttgctttc
     8221 ctactcctct atctggggaa ttagtcttag cggggaagat ggaaggcgat tattagaagt
     8281 tgccaaccaa tcgcaaggta acactggttt ggttcttaaa cctacaattg gaatgaagaa
     8341 gttgattgaa gtgccgactg tatcaggctt ttctagttgg ggacctacgg tcaacttaga
     8401 attgaagcca gagattgtgg cgcctggaga ggatgtttat gcaaccttga atgacaatcg
     8461 ttatggctcc atgtcaggga cttcgatggc ttcgccaatt gtggcgggtg ccagcgcact
     8521 tttattgcca cggattcgcc aaatgacacc accagaaggc atgactagga tggatttgct
     8581 gagaatcatt ttgatgaata ctgctacccc tttggttgat gttctagatt catctgggca
     8641 tgctttggaa aattctccga gacaacaagg tgctggtctg ttgcagattg atagagcctt
     8701 tgaaacagat gtgattcttc accaccgtct aaaaggaggg gtggaattaa aagaaatagg
     8761 gcgtgagaca gaatttgagg taaccttgga aaatctggga aaccaacaaa gaagctttgc
     8821 tatttcagct gggaaagtgt tgactagtca agatgttcct gttgatagaa taggacgttc
     8881 tggaaaagta gttaaagaga ttcatgcgac agaaatcaag gggtcgagta ttcatctttc
     8941 tgagcaatcc attcaactag gtccaaaaga gaagagaacc attcgcctga aattggatgc
     9001 aggagaagcg aaagaccagt ttgcagaagg gtatatttac ttcaaatcat tgacggatgg
     9061 gcagtctgac atcagcatac cttattttgg ctttgttggt gactggtcaa aagaaaggat
     9121 tgttgatgca ccggcttggg aaaccagttc taaattgaaa ctgacctcag ttttatctag
     9181 ctataaacac aataagtctg ggcgctatat tgaacttggg cgtgaaaaga ttcaagataa
     9241 tcaatcgcct ctaaatccag acaatatcgc aattcaaaac cagcattcgg atagtcagat
     9301 tgggaatgcc tttgttcgat ttgcactatt gagggatatt accaactatg atttggatat
     9361 tgtaaaagaa gctacagaag acgctcctgt tttaaggcga attgatacag gaactatgct
     9421 gtctcgtgtt cgctatgtag attattttga aagtctttcg gagtatttca agcttcgtac
     9481 tccgatagaa ttgcaccgtt gggatgggaa agtgtacgat gcaagcaagg atgaaaacat
     9541 accagcaccg gaaggacaat atttctttag attgcgggta aaaaataaag aaaatggggc
     9601 ctatcagtat acctatctgc cagtcaaaat tgataatcaa aagccagaaa ttgtcgcgat
     9661 tgataccaat cgtctgacca gccatagaga actagtcgtc acagccaagg ataataataa
     9721 agtttgggag gttcgagcta atctaaatgg agaagatctt cttgttgaaa aggttgtaga
     9781 tgatgcaggt caactacatt accatctcaa agaagtggaa ctgccactcg atgctaaaaa
     9841 tcatctccgt gttgaggtga tggatatagc gggtaatgtg gttgctgttg aaaaagactt
     9901 ggtggcgcct gtaatccagt ttaaaaattt ggaagatttg atggcaattc gcagtaagaa
     9961 aactgtagaa attaaagcga acgtctcagc acaagtttcc aatgtacagg ctaacttgga
    10021 tgctcaggct gtgaattact ctctggagaa tggtcaacta tctctccaga ttccagagca
    10081 atcagatgga cgtcatagtt ttgaattaat cttgaaagat aaggatggca agcttattta
    10141 cactaaaacc ttgaattatt tggtagataa tgaaaagccg acgattgatc ttgatattga
    10201 aaaagatgaa gaggatgaag aggttatcca gattggcaaa aacgggcgtt ttacactgaa
    10261 ggggaaagtg agtgataatg ttagtcttcc taagaacatc aaactttact attccaatct
    10321 agacattggt aagggagagc tgaagatcat agatgtaaaa gaagatggta gttttgagca
    10381 agacttcttt aaatctgatt ttcctagagc tatcatgtta actgcggtag atgagaaggg
    10441 aaataaatta aaggaccttc gtatcaatac aagtccagag agtctggatg aggaggagga
    10501 aacagaagtt ccgattactg ttaataactg gttgattgac ccgattcgtt tcaataaaga
    10561 aagtcttggt cgtgagctag atagcgggtt ggtcgacttc aaaaagcaag aagatgggac
    10621 ttatctattt acatttgaaa tagaagcaga aacggatcaa gctcattctg tgcgcatcaa
    10681 tggtggtgaa aaacgctatt ttgaggatgg caagttgacc tatccagtta ccctgattga
    10741 agagggcaat gttgtggata taagtgtgta caatgaggcg gatgagttga cttacacgaa
    10801 gaaatatcag atgttggttg atacagaaaa ccctgtttta cagctggaaa atgaggtgct
    10861 accattggaa agacaggtag tggatagtga agaagatgaa gacgaggaaa atcagtatgc
    10921 aggagttctc ctggcagatg ctgatgggca cttgacactt accggaagcg ccaaggataa
    10981 tggaatttat tggtccttga agatcaacga agattttgta gctcgaggag gtttctggag
    11041 gcagtatgga aataatgaaa aagcctttcg ctatgagcta catagtctga aagacgggga
    11101 tacagtcaaa cttgatttga gtgacagttt tggaaatgct cttgtgaaga aatataaggt
    11161 acggttaaat gacaaggaag tatctgagca ggttcctgaa aaagaccttc atgtggagca
    11221 atcagataag gatcaagccc ctagcattcc tgttccgaaa tcggaagcgc atgtaccaat
    11281 gccaaaagaa gagaattcgc ttgctccgca aacagaatcc actgaaatcg cgttgttaac
    11341 tggcgataca agagaagatg gtgtggagca tttggtgaga tttacaaaac atgaggaggc
    11401 actaggaatt tctgatgaga gaatagaagt gtccgtacct cacagagaat tctttgaaag
    11461 aagcggaaga ggagagacag gtgcactagc ggcggatact agtgggaaac tgcctcaaac
    11521 tggagatagc cttggaagtg tctttataag tgctttgtta ggtctatttg gtggagctat
    11581 ggtcctcgga aatctgaagc gaaaagaata gcaaaaaacc taggaagatt cctaggtttt
    11641 ttagaatatt aaaaggcggt agacggattt gaaccgacga tcaagctttt gcagagccgt
    11701 gccttaccac ttggctatac cgccacaaca aagaatattc taccgaaaaa aaagctgaaa
    11761 gtcaagaaaa atctagtgac tgtttgtgac tgataacaaa tctataaaac agttgcgaaa
    11821 acataggaga atacgacgaa aaagatagga gtactttttg agtattgatg gctagtacca
    11881 ctctctttgt ttgatagaaa aagagtggag caaaaagaat taagaaacac tttgggtaag
    11941 tgaattcatt tttactgcaa ggaatgttaa gggaacagaa ttacgataat agtgcacctc
    12001 tttaagaaag atttggaggt gatagagttt atttttgcta aataaagatt aaaccaagaa
    12061 gaatattcca tctaaaacgg ttgttttcat ttacagtata ttgcaaagat aactcaggca
    12121 cttgattaga ttaactgaaa aaatgtggga acatgatata ataaattgag taagagattg
    12181 aaaaatagat agg
//
LOCUS       DASFSW010000059        12131 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.059, whole genome shotgun sequence.
ACCESSION   DASFSW010000059 DASFSW010000000
VERSION     DASFSW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.059"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(29..4786)
                     /locus_tag="U1099_001175"
     CDS             complement(29..4786)
                     /locus_tag="U1099_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEM4867500.1"
                     /translation="MKKKETFSLRKYKIGTVSVLLGAVFLFAGAPSVAADELTSLVET
                     KVEATVPDVIVSESASESPVVEELVDTSVEATPTDVTTTDNVEETLGSEALENITNTE
                     VEATQPAVETPAISEKKVEEDEKLAVADETTAITNQEEAKPQNIDSNTIITVPKVWDS
                     GYKGEGTVVAIIDSGLDVDHDVLHISDLSTAKYKSEKEIEAAKEAAGITYGEWFNDKV
                     VFGYNYVDVNTVLKEEDKRSHGMHVTSIATGNPTQPVAEQLMYGVAPEAQVMFMRVFS
                     DLKATTGAALYVKAIEDAVKLGADSINLSLGGANGSVVNMNENVTAAIEAARRAGVSV
                     VIAAGNDGTFGSGHSNPSADYPDYGLVGTSSTARDAISVASYNNTTVGSKVINIIGLE
                     NNADLNYGKSSFDNPEKSSVPFEIGKEYEYVYAGIGQASDFDGLNLTGKLALIKRGTI
                     SFSEKIANATAAGAVGVVIFNSRPGEANVSMQLDDTAIAIPSIFIPLEFGEALAAKPY
                     KIAFNNETDIRPNPKAGLLSDFSSWGLSADGELKPDLAAPGGAIYAAINDNDYANMQG
                     TSMASPHVAGAAVLVKQYLQATYPTKSPQEIEALVKHLLMSTAKAHVNKETTAYTSSR
                     QQGAGIIDTAAAISTGLYLTGEDGYGSITLGNVEDTFSFTVKLHNITNEDKTLNYSTQ
                     LTTDTVQNGLITLAPRLLAEIPGGKVTVKANSSTTVTINVDASSFAEELTGLMKNGYY
                     LEGFVRFTDVADGGDIVSIPYVGFRGEFQNLAVLEEPIYNLIADGKGGFYFEPVTAQP
                     DTVDISHHYTGLVTGSTELIYSTDKRSDFAIKTLGTFKNEAGYFVLELDESGKPHLAI
                     SPNGDDNQDSLAFKGVFLRNYTDLVASVYAADDTERTNPLWESQPQSGNKNFYSGDPK
                     NPKSSIIYPTEWNGTDSEGNALADGKYQYVLTYSSEVPGAAVQTMIFDVIIDRESPVI
                     TTATYDETNFTFNPRPAIEKGESGLYREQVFYLVADASGMTTIPSLLENGDVTVSDNK
                     VFVAQNDDGSFTLPLDLADISKFYYTVEDYAGNISYEKVENLISIGNEKGLVTVNILD
                     KDTNSPVPILFSYSVTDETGKIVAELPRYAGDTSVLKLPFGTYTFDLFLYDTEWSSLA
                     GETKAVVTISEENSTAEVNFYVTLKDKANLLVDIDALLPSGSTIQLVTADGQTIQLPN
                     AKYSKTDYGKFVPVGTYTILPTLPEGYEFLEELDVAVLANQSNVKKLTLINKVALKEL
                     IAELAGLEETARYYNASPELQTAYDKALEDANAVYANKHNQAQVDSAVASLVAAREQL
                     NGQATDTEKLIAEVSNYTPTQANFIYYNAENTKQIAYDTAVRSAQLVLNQENVTQAVV
                     NQALADLLAAKANLDGQKTDISALRSAVSVSSVLKATDAKYLNASENVKQAYDQAVEA
                     AKAILADESASQASVDQALAVLTSAQAELDGVATSTNDAKEPANTATDKKDEGTVTPP
                     PIDSEKVDVQAPPVKDTGNSGHVPIGQKPNPQPTLPRPVTLQASLSSPNQEKQVTQLP
                     NTGDNDTRYYLVLGVIIGLGTLLVSKRRHKEEV"
     gene            5239..5571
                     /locus_tag="U1099_001176"
                     /pseudo
     CDS             5239..5571
                     /locus_tag="U1099_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935801.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5619..7157)
                     /locus_tag="U1099_001177"
     CDS             complement(5619..7157)
                     /locus_tag="U1099_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEM4867501.1"
                     /translation="MENHRKEVSFISQSILYSVLRGSLVGIVAGLVVVAFRLAIEKLF
                     SVFTHLYSLATDNAIYLLLIGGLYLVIALLVGKLIKDEPNAKGSGIPQVEAELKGIMD
                     LNWWSVLWRKFIGGVLSIASGLMLGREGPSIQLGAVTAKGVSVLLKSSEMERRSLIAS
                     GAAAGLAAAFNAPIAGLLFVVEEVYHQFSRLVWVSALAASITANFISLHVFGLMPVLH
                     MPKDLQLLSLDQYWIYILLGVFLGLAGYVYEIVILNIQDFYTLLSKIIPLPVPYYSVF
                     AFLFIIPIGYFFSNLLGGGHHLILELPYLEQGLLTLAILILIRFVWSMISYGSGLPGG
                     IFLPILTLGSLLGLFVARFFLDIGFISQEQMLLFIVLGMAGFFAAISKAPLTAIILVT
                     EMVGSLNQLMVIGLVALSSYVMMDLLGGAPVYEAMLEKILPEEVEQQEHMTLIEIVVN
                     ETLDQIFVSELRLPDSCLITSQIHHGKSRIVKGNSQLSMGDSLLVAVPISQIHTVRRI
                     FEGD"
     gene            complement(7271..8341)
                     /locus_tag="U1099_001178"
     CDS             complement(7271..8341)
                     /locus_tag="U1099_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4867502.1"
                     /translation="MKRLYSFFVGIIAIVLVLWGISYKLEADSTQGTTDKLVIYNWGD
                     YIDPELLAEFTAETGIQVDYQTFDSNESMYTKIKQGGTTYDLTIPSEYMISKMTEENM
                     LIKLDKSKIEGIENIDPRFMGLSFDKNNDYSIPYFWGTLGIVYNETMVENPPQEWEDL
                     WSEEYRDNIMLIDGVREVMGFGLQSLGYSLNSNNPTEIEKAAEHLYNLTPNVKAIVAD
                     EIKGYMIQDAAAIAVSFSGEASEMLDGNENLRYVVPSKGSNLWFDNMVIPKTAKNIDG
                     AYAFMSFMLRPENALRNAEYVGYSTPIPAAKAMLDEETQNDESFYPSEETMKKMEVYD
                     NLGAEMLGMYNDLYLQFKMYRK"
     gene            complement(8341..9111)
                     /locus_tag="U1099_001179"
     CDS             complement(8341..9111)
                     /locus_tag="U1099_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867503.1"
                     /translation="MKKFANIYLTVAFLLLYLPIFYLIFYAFNEGGDMNGFTGFTLEH
                     FSSMLGDSRLMLILAQTFLLAFLSSLIATIIGTFGAIYIYQAKPRFQNALLSVNNILM
                     VAPDVMIGASFLILFTMIGFQLGFVSVLLSHIAFSIPIVVLMVLPRLKEMNADMISAA
                     YDLGATQPQMLKEIMLPYLTPAIIAGYFMAFTYSLDDFAVTFFVTGNGYSNLSVEIYS
                     RARQGISLEINALSTLVFLFSILLVVGYYFISREKEAN"
     gene            complement(9111..9911)
                     /locus_tag="U1099_001180"
     CDS             complement(9111..9911)
                     /locus_tag="U1099_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867504.1"
                     /translation="MKKTSRLFAIPYALWVFLFVLAPVALIIFKSFFDIHGNFTLANY
                     QTYFNSPNMTYLRMSFNSIFYAGIITLVTLLVSYPTAYFLTKLKHRQLWLMLIILPTW
                     VNLLLKAYAFIGIFGQHGSINQFLEFIGLGAQQILFTDFSFIAVAAYIEIPFMILPIF
                     NALDDLDKNLINASRDLGATPWQTFTKVIFPLSMNGVRSGVQAVFIPSLSLFMLTRLI
                     GGNRVITLGTAIEQHFLTTQNWGMGSTIGVVLILAMLLIMWMTKERKK"
     gene            complement(9895..11049)
                     /locus_tag="U1099_001181"
     CDS             complement(9895..11049)
                     /locus_tag="U1099_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867505.1"
                     /translation="MKKPIIEFKNVSKVFEDSGTTVLKDISFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDASSGDIYLDGQRINDVPTNKRDVHTVFQSYALFPHMTVFENVAF
                     PLKLRKIDKAEIERRVTEALQMVRLSGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGRMIEDYLVEFNGKRFEAVDGGMRPNEEVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIVGEVIG
                     LSFEPEDIHIMRLNETEEEFDARIEEYVEVEEVEDGLINAIEEERNEENL"
     gene            complement(11088..11996)
                     /gene="murB"
                     /locus_tag="U1099_001182"
     CDS             complement(11088..11996)
                     /gene="murB"
                     /locus_tag="U1099_001182"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEM4867506.1"
                     /translation="MKDKIRLELEGIDIRFDEPLKEYTYTKVGGAVDYLAFPRNRYEI
                     VRIVEFAKREGIPWQVLGNSSNIIVRDGGIRGFVIRMDKLNSVTVSGYTIEAEAGANL
                     IETTKVALFHSLSGFEFACGIPGSIGGAVYMNAGAYGGEVAHILVSAQILTPAGYVET
                     LDNRELRFGYRSSILQENGAIVLSAKFALKPGNHTVIQQEMARLTHLRELKQPLEYPS
                     CGSVFKRPLGHFAGQLIMDAGLKGYRIGGVEVSEKHAGFMVNIENGTASDYENLIAHV
                     IEVVEKSSGITLEREVRIIGDPADTL"
BASE COUNT         3637 a         2596 c         2322 g         3576 t
ORIGIN      
        1 gattttcgaa gagtatgact atattttctt agacttcttc tttatgacgt cgtttgctta
       61 ccaacagagt ccctagccca ataatgacac caagaacaag atagtatctc gtatcattgt
      121 ctccagtatt tggtagttga gtcacctgtt tttcttgatt agggctagat agactagctt
      181 gcaaagtgac cggacgaggt aaagttggtt gagggtttgg cttctgacct atcggtacat
      241 gccctgaatt cccagtatct tttacaggag gtgcctgtac atcaactttt tctgagtcta
      301 taggtggagg cgttacagtg ccttcatctt ttttgtcagt ggcagtattt gctggctctt
      361 tggcatcatt tgttgaagta gcaacaccat ccagttctgc ctgagcgctt gtcagaacgg
      421 ctagagcttg atcgacactt gcttggcttg cagattcatc agctagaatc gctttcgctg
      481 cttcaactgc ctggtcataa gcttgtttca cgttttcaga tgcattgaga tacttagcat
      541 ctgtcgcttt taatacggaa gaaacggata ctgcgctacg aagggctgaa atatcagtct
      601 tttgaccatc taagttggct ttcgccgcta acaagtccgc caacgcttgg ttgacaactg
      661 cctgtgttac attctcttgg ttcaatacaa gttgtgctga acgaacagct gtatcatagg
      721 caatttgttt ggtattttca gcattgtaat aaataaagtt tgcctgagtc ggtgtgtagt
      781 ttgatacttc agcaattagt ttttccgtat cggtagcctg accgtttagc tgttctctcg
      841 ccgccacaag actggcaact gctgaatcta cttgtgcctg attgtgtttg ttggcatata
      901 ctgcattggc atcttctaat gctttatcat aggcagtttg aagttctgga ctagcattgt
      961 aataacgcgc tgtttcttca agtcccgcaa gttcagcaat cagttctttc aaagcaactt
     1021 tattaatcaa ggttaatttc ttaacatttg actggtttgc aagtactgct acgtctaatt
     1081 cttccaaaaa ttcatagcct tctgggaggg ttggaaggat agtgtaggta ccaactggta
     1141 caaatttacc ataatcggtc ttagaatatt tagcatttgg tagctgaata gtctggccat
     1201 cagcagttac cagttggatg gttgaaccag aaggtagtaa tgcatcaata tctaccagca
     1261 agttggcctt atctttcaaa gtcacataga aattcacctc ggcagtgcta ttttcttccg
     1321 aaatcgtcac gactgctttt gtttcacctg ctaggcttga ccattctgta tcatataaga
     1381 ataaatcaaa ggtgtaagta ccaaatggta gcttaagaac gctagtatcg ccggcatatc
     1441 gtggtaattc tgcaacaatc ttgcctgttt catctgtgac tgagtaagag aaaagtattg
     1501 gtacaggact atttgtatct ttatcaagaa tattgacagt taccaaccct ttttcattgc
     1561 cgatactgat cagattctct actttttcat agctgatgtt accagcataa tcctctactg
     1621 tgtagtagaa ttttgaaata tctgcaaggt caagaggcaa tgtaaaggag ccatcgtcgt
     1681 tttgtgccac aaataccttg ttatcagaaa cggttacatc accattttct aataaggaag
     1741 gaatagttgt cataccgctt gcatctgcta caagatagaa tacttgctcg cgatatagcc
     1801 cggattctcc tttttcaatg gctggacgag ggttaaatgt aaagtttgtt tcatcatagg
     1861 tagctgtggt gataactggt gattctctat cgatgataac gtcgaaaatc atagtttgta
     1921 ctgctgcacc tggaacttca gatgagtagg tcaaaacgta ttgatactta ccatctgcta
     1981 aagcatttcc ctcgctgtct gtcccattcc attcagtagg gtaaataatg cttgattttg
     2041 gatttttagg atcaccacta tagaagttct tattgcctga ctgtggttga ctttcccaaa
     2101 gtggatttgt tcgttcggta tcatctgcag catagacgct tgcgactaaa tccgtataat
     2161 ttctcaagaa gacacctttg aaagcgagcg aatcttggtt gtcatcccca tttggcgaga
     2221 tagctaaatg aggcttacca gactcatcaa gctctaaaac aaaatatcct gcttcatttt
     2281 taaatgtacc aagtgtcttg atcgcaaagt cagatcgttt gtctgtagaa tagattaact
     2341 ccgtacttcc tgtaacaaga cctgtgtagt gatggctgat gtcaacagta tctggttgtg
     2401 ctgtaacagg ttcaaagtag aagcccccct taccatcggc aataagattg taaatcggct
     2461 cttctagaac ggctagattt tggaattccc cacggaaacc aacgtatgga atgctgacaa
     2521 tatcaccgcc atcggctaca tctgtaaatc gaacaaaacc ttcaagatag taaccgtttt
     2581 tcattaaacc tgtcaattct tctgcaaagc ttgatgcatc gacattaatt gtaacagttg
     2641 tacttgaatt ggctttcaca gtcaccttac cgccaggaat ctctgctaat agacgcggag
     2701 ccaaggtgat caatccgttt tggacagtat ccgttgttaa ttgcgttgag tagtttaaag
     2761 tcttatcttc gtttgtaatg ttatgaagtt tgaccgtaaa gctgaatgta tcctcaacat
     2821 ttcccaaggt aatgctgcca taaccgtctt cgccagtcaa atataaacct gtagaaatag
     2881 ctgccgcagt atcgatgata cctgcacctt gttgacgaga ggaagtgtag gctgttgttt
     2941 ctttgttcac atgcgcttta gcagtagaca taagcaagtg ttttactaag gcttcgattt
     3001 cttgagggga cttagtaggg taagttgcct gtaaatattg ttttactagt acggctgctc
     3061 ctgctacgtg tggtgaagcc atacttgttc cctgcatgtt ggcatagtca ttatcattga
     3121 tggctgcata aatagcacca cctggagcag ctaagtctgg ttttagctcg ccatccgctg
     3181 atagacccca acttgaaaaa tctgaaagaa gacctgcttt ggggttagga cgaatgtctg
     3241 tttcgttatt gaacgcaatc ttatatggtt tagccgctaa agcttcaccg aattccaatg
     3301 gaatgaagat agatggaatt gcgatagctg tatcatccag ttgcatgctc acattggctt
     3361 cacctgggcg gctattgaaa ataacgaccc ctactgcccc tgcagctgtt gcattggcta
     3421 ttttttctga gaaactgatg gttcctcgtt taataagcgc aagttttcca gtcaaattta
     3481 aaccatcaaa atccgaagct tgaccgattc ccgcatagac atattcatat tctttcccga
     3541 tttcaaatgg tacggaactt ttctctggat tatcaaacga acttttaccg taattcaagt
     3601 cagcattgtt ttctaagcca atgatattaa ttactttact accaaccgtt gtgttattgt
     3661 aagaagcgac agaaattgca tcgcgagctg ttgaagatgt accgaccaag ccataatctg
     3721 gataatcggc tgacggatta gaatgaccgg aaccaaatgt tccatcatta ccagctgcaa
     3781 taacaacaga aacccctgca cgacgagcag cctcgattgc tgcagtcaca ttttcattca
     3841 tgttaacaac agagccatta gctcctccca ggctgaggtt gatgctatct gcacctaatt
     3901 ttacagcatc ttcaatcgcc tttacgtaca atgctgcgcc tgttgtagct ttgaggtctg
     3961 agaatacacg cataaacatg acttgcgctt caggagctac accatacatt aattgttcag
     4021 cgactggttg tgtcggattt cctgtggcaa tactcgttac gtgcataccg tgtgagcgtt
     4081 tgtcttcttc tttcaagaca gtattcacat caacatagtt ataaccaaat acaaccttat
     4141 cgttaaacca ttcaccgtag gtaattcctg ctgcttcttt agctgcttct atctcttttt
     4201 ctgatttata ttttgcagtt gagagatctg aaatatgcaa tacatcgtgg tcaacgtcaa
     4261 gacctgaatc tatgattgca actactgttc cttcgccctt gtaaccacta tcccaaactt
     4321 taggtactgt aatgatcgta ttgctatcaa tgttttgtgg ttttgcttct tcctgattag
     4381 taatagcagt agtctcatct gctacggcaa gcttctcatc ttcttctact tttttctctg
     4441 aaatagctgg agtttctaca gctggttgag tcgcttctac ttctgtattt gtgatgtttt
     4501 caagagcttc tgagccaagt gtttcctcta cattatctgt agtggttaca tcagttgggg
     4561 tagcctccac agaagtgtca actaactcct cgactacggg actttctgag gctgattcgc
     4621 tgacgattac gtcaggaaca gttgcttcca ccttagtctc tacaaggctt gtcaattcat
     4681 ctgcagctac cgatggtgca cctgcaaaca aaaaaactgc acccaaaaga acagacacag
     4741 ttccaatttt atacttccga agtgagaaag tttctttctt tttcataact tttcctccta
     4801 gtttttctcc ttcattattg gcaaaaccaa taagtatatt aaaacataaa actatcagca
     4861 ttgcaagtat attctgaaaa ttcagttatt tattcaaact ataactaggt atcaataata
     4921 tctataacca tagatacaga taatacacac taaaaaggga gtgagcaaga catccatgat
     4981 ggctccgtcc ttacccacag aataatgaaa ataagcataa agccctcact aagattatat
     5041 acctagtgag ggccttgcgg ttggtacatt attgatgtga attatgatga caaaactgct
     5101 ccagcctact gataagatcc acaatataca ctcaagaata tgactaaaat tcagtagata
     5161 ggctctctaa cttataagga aggaattgtc atagataatc atgtgtagat caagtcgata
     5221 ttgttgtttg tattcccctc aacttgtcca aagcgttact tagctacatt tgatacaacg
     5281 actaaaggtc tacgtgctta ccacgatttt ctcagtcaat ttcatgtgga agctgttggt
     5341 atggaaagca ccggtgtcta ttggcgacct gtctggcatg ctctatgtga ggacttcgag
     5401 ctaatacttg ctcaaccagc ccacatgaag gccattacag gtcagaaaac tgacaagaaa
     5461 gacgcttact ggactgccaa atgaatgcgg attggtctgc ttcctcggag tttcgttccc
     5521 gatgaaatca ttcctgaatt gagagagttg accagacaac agaaacatta taatattggg
     5581 acttttctca tctctttgtt aagacaatct aaaaaatgct aatctccctc aaatatccta
     5641 cgaacggtat gaatttgaga gattggaact gcaacgagca aggaatcccc catagaaagt
     5701 tggctatttc ctttcacaat ccttgactta ccgtgatgaa tttgactggt gatcaagcat
     5761 gaatccggca aacgtaactc gctaacaaat atttgatcca atgtttcgtt gacaacaatc
     5821 tctattaagg tcatgtgttc ttgctgttct acttcttctg gcagaatttt ttccaacatt
     5881 gcttcataga ctggcgcgcc acctaagagg tccatcatca cataggaact caaagctacc
     5941 aacccgatga ccatcaattg attcaaactt ccaaccattt cagttactaa aataatagca
     6001 gttagaggtg ctttggaaat cgctgcgaaa aagcctgcca ttcccaatac aataaacaat
     6061 agcatctgct cctgactgat gaaaccaata tcaaggaaga accgtgcgac aaaaagccca
     6121 aggagacttc ctaaggttaa tatgggcaaa aagatgcccc ctggtagacc agaaccatag
     6181 gaaatcatgc tccatacaaa tcgtattaaa atcaagattg ccaatgtcag tagtccttgt
     6241 tccaaatatg gaagctctaa aatcaaatga tgcccgccac ccaacagatt agagaaaaaa
     6301 taaccaatcg gtataataaa caaaaaggca aatacactgt agtatggaac tgggagagga
     6361 atgatttttg agaggagggt atagaaatct tgtatattga ggataacaat ttcatacaca
     6421 tatccagcaa gtccaaggaa aactccaagt aagatataga tccaatattg gtcaagagat
     6481 agtaactgta agtccttcgg catatgcaaa acaggcatta atccaaaaac atgtaaggaa
     6541 ataaagttcg cagtgatact ggctgccagg gctgagaccc ataccagacg tgaaaactga
     6601 tgatagactt cctccacaac aaataaaagg cctgctattg gtgcattgaa agctgctgcc
     6661 aaacctgccg cagcgccact agcaatcaaa ctccttcttt ccatttcact ggatttaaga
     6721 agaactgata cacctttagc cgttacagct cctaattgaa tactgggacc ttcacgcccc
     6781 agcattagac cagatgcaat ggataaaaca ccgccgataa attttctcca aaggactgac
     6841 caccagttta aatccataat tccttttagt tctgcctcca cctgaggtat accagaacct
     6901 tttgcatttg gctcgtcttt gatcaacttg ccaacaagta aagctatgac cagataaagc
     6961 cctccaataa gaagtaaata gattgcattg tctgttgcca gagaatagag gtgcgtaaag
     7021 acagaaaata gtttttcaat ggctaagcga aatgcaacga ccactaaacc agcgacaatg
     7081 ccaactaaac taccacgcag aaccgaatag aggattgatt gagaaataaa actaacttct
     7141 ttccggtgat tttccattgg gatttccctt cctttctata gtatgtatga ccaaaaaata
     7201 ggagcaataa gtacttagta aagaacctca ctcccattct tttatttata ttgctacctg
     7261 tccaatcaga ctatttccga tacatcttga attggagata gaggtcatta tacattccaa
     7321 gcatctctgc tccaagatta tcataaactt ccatcttctt catcgtttct tctgaaggat
     7381 agaaggattc gtcgttctga gtctcctcat ccagcatagc cttggctgca ggaattggtg
     7441 ttgagtagcc aacatattct gcattgcgaa gcgcattttc aggacgaagc ataaaactca
     7501 taaaggcgta agctccatca atattttttg ctgtttttgg aatgaccatg ttatcaaacc
     7561 acaggttaga accttttgat ggtacaacat agcgcagatt ttcatttcca tctaacattt
     7621 cactcgcctc acctgagaac gaaacagcaa ttgctgccgc atcttgtatc atataacctt
     7681 ttatctcatc agcaacaatt gctttgacat ttggagttaa attataaaga tgttcggctg
     7741 ccttttcaat ctcggtagga ttgttagaat tgagactata gcccagtgat tgtagaccaa
     7801 agcccatgac ttcacgcaca ccatcaatta acatgatatt gtcacggtat tcctctgacc
     7861 acaagtcttc ccattcctga ggaggatttt ctaccatggt ttcattgtaa acaatgccca
     7921 aggttcccca gaaatatgga atagaatagt cattattctt atcaaagcta aggcccataa
     7981 atcgtggatc gatattttct atcccttcaa tcttagactt atctagcttg attaacatat
     8041 tttcttctgt catcttagaa atcatgtact cagatgggat tgtcaaatcg tatgttgttc
     8101 caccctgctt aatcttggtg tacatcgatt cattggagtc aaaggtctga taatctacct
     8161 gaatacctgt ttcagcagtg aattctgcga gtaattctgg gtcaatataa tcaccccagt
     8221 tatagatgac aagtttatca gttgtccctt gggttgaatc agcttctaac ttataactaa
     8281 ttccccataa aactaggaca atagctataa ttcctacaaa aaatgaatac aatcgtttca
     8341 ttagttggcc tccttttcac gtgagatgaa ataataaccc actaccaata agatagagaa
     8401 aaggaaaaca agggttgata gggcattgat ttccaaagaa atcccttgac gagcccgtga
     8461 gtaaatctct acagataggt ttgagtaacc atttcccgta acaaagaatg tcacggcaaa
     8521 gtcatccaag gaataggtaa aggccatgaa gtagcctgca atgatggctg gtgttaggta
     8581 tggcaacatg atttctttca gcatttgtgg ctgagttgcc cccaaatcat aagctgctga
     8641 aatcatatcg gcattcattt ccttcaagcg tggcaaaacc atcaatacta cgattggaat
     8701 agaaaaagca atatgactga gcaatactga gacaaagcca agctggaaac caatcatggt
     8761 aaagagaatt aggaaactcg ccccaatcat gacgtctgga gctaccatca agatattatt
     8821 gacagacaag agtgcatttt gaaaacgagg ttttgcctga tatatataga tggcaccgaa
     8881 agttccgata atggtcgcaa tcaaagaact caagaaggcc aacaagaagg tttgagccag
     8941 aatcagcatt agtcggctat cgccaagcat agatgaaaaa tgctctaagg taaagcctgt
     9001 aaagccattc atatctccgc cttcattaaa ggcataaaaa ataagataga aaatcggtaa
     9061 gtagagaagg agaaaggcaa ctgttaagta gatatttgca aatttcttca ttatttcttc
     9121 ctctctttcg tcatccacat aatcaagagc atggccaaaa tcaagaccac acctatggtt
     9181 gaacccattc cccagttttg tgttgtgaga aagtgttgtt ctatagctgt accaagcgtg
     9241 atcactcggt taccgccaat caaacgggtc aacatgaaga ggctcagact tggaatgaaa
     9301 accgcctgta caccagaacg gacaccattc atagaaagag gaaaaataac ttttgtgaag
     9361 gtctgccaag gagttgcccc caaatcacga ctggcattga ttaggttttt atccaaatca
     9421 tccaaggcgt taaagatcgg caaaatcata aacggaatct cgatataggc tgcaacagca
     9481 ataaatgaga aatccgtgaa aagaatttgc tgagctccta gaccaataaa ttctaggaac
     9541 tggttgatag agccgtgttg tccaaaaata ccgataaagg cataggcttt cagtaaaaga
     9601 ttgacccagg ttggcaagat aatcaacatg agccagagct gtctatgctt gagtttggtc
     9661 aagaaatagg ctgttgggta ggaaaccaaa agcgtgacca gagtaataat acctgcgtaa
     9721 aaaatggagt taaaactcat tcgcagatag gtcatattgg gcgagttaaa ataggtctgg
     9781 taattggcca aagtgaaatt gccatgaata tcaaagaaag atttgaagat aatcaaggca
     9841 actggagcga gtacaaagag aaagacccat agggcatagg gaattgcaaa aagcctagag
     9901 gttttcttca ttgcgttcct cctcaatggc attaatcaag ccatcttcta cttcttccac
     9961 ttccacatat tcttcgatac gggcatcgaa ttcttcctct gtttcattga gacgcatgat
    10021 atgaatatct tctggctcaa aacttagtcc aatcacttct ccgacaatag ccttacgagt
    10081 tgagtgaatc atccactcat taccgaggtc gtcataggcg atgatttcat agtgaacccc
    10141 acggaagagt tgagtatcca cttttacctg caatttgcct tcctcaggta gggtaatctg
    10201 caaatcttct ggacggataa caacttcaac ttcttcatta ggacgcatac cgccatcgac
    10261 agcttcaaag cgtttgccgt taaactcaac cagatagtct tctatcatac gacctggaag
    10321 gatattggat tcaccgatga aggtagcaac aaaatggttg attggctcgt cgtaaatgtc
    10381 cactggcgtg ccagactgaa caatttcacc ttcattcatg acaaaaatcc agtcactcat
    10441 ggccagagct tcttcttggt catgggtaac gaagacgaag gtaattccta gacgttgttg
    10501 caattcacgc agttcgtact gcatttctgt tcttagtttc aagtccaggg cagaaagtgg
    10561 ttcatctagc aagacaacgc gaggctggtt gataatcgca cgtgcaatag caacccgctg
    10621 gcgttgacca ccagacaatt tctgaatgga acgattttca taaccagaaa gtcggaccat
    10681 ttgtaaggct tctgtcacac ggcgttcaat ttcagccttg tcaatctttc gtaatttaag
    10741 aggaaaagcc acattttcaa agacagtcat atgtgggaaa agggcatagg actggaaaac
    10801 agtatggaca tcccgtttat tggtcggaac atcattaatc cgttgaccgt ccagataaat
    10861 atcacctgaa ctcgcatcca ataaaccagc aataatgttc aaaattgttg atttccctga
    10921 accagaagct cctaaaaggg tataaaattt cccctcttcc aattcaaaac taatatcctt
    10981 taatacagtt gttccactgt cttcaaatac ttttgaaaca ttcttaaatt caataattgg
    11041 ctttttcaat tctcataaat tccttctttt ttgtaagagc ctgatggcta gagtgtatca
    11101 gcaggatcgc cgataatacg aacctctctc tccagagtaa ttcctgaaga tttttcaaca
    11161 acttcaatta catgggcaat caaattttcg taatcagatg ctgtaccatt ttctatattg
    11221 accataaagc cagcgtgttt ttcagaaact tcgactccac caattcgata accttttaaa
    11281 cccgcatcca taatcaactg tcctgcgaag tgaccaagtg gacgcttgaa gactgaacca
    11341 caagatggat attccaaagg ttgcttgagc tcgcgtaaat gtgtcaaacg agccatttct
    11401 tgttgaataa ccgtatgatt tcctggcttg agggcaaatt ttgcagacaa aacaatcgca
    11461 ccgttttcct gcaaaataga agaacggtag ccaaatcgta actcacgatt gtccaatgtt
    11521 tcgacataac ctgctggagt caaaatctgc gctgaaacta gaatgtgtgc cacttcacct
    11581 ccgtaagcac ctgcattcat gtagacagca ccaccaatgc ttcccggaat accacaagca
    11641 aattcaaatc ctgataaaga atggaaaagt gcgacttttg ttgtttcaat cagattggca
    11701 cctgcttctg cttcaatggt gtatcctgat acagtaacgg agttaagttt atccatacgg
    11761 ataacaaaac cacgaatacc gccatctcgg acaatgatat tacttgaatt tcctaatact
    11821 tgccatggaa taccttcgcg tttagcaaat tctactatac gaacaatctc gtaacgattc
    11881 cgcggaaaag ctagataatc aacagctcct cctacttttg tataggtata ttcttttaaa
    11941 ggctcatcga atcgaatatc gatgccttct aattcaagcc taattttatc tttcataacc
    12001 ttctcatttc aatttaagat atacaggatt tattatacca aaaattacag gagaaaacga
    12061 ataaaattca acaaaaatac tttcacaaac caaattaatt tgccccaata ttttgaattg
    12121 gagaatggaa t
//
LOCUS       DASFSW010000060        11993 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.060, whole genome shotgun sequence.
ACCESSION   DASFSW010000060 DASFSW010000000
VERSION     DASFSW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11993
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.060"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1125
                     /locus_tag="U1099_001183"
     CDS             <1..1125
                     /locus_tag="U1099_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM4867507.1"
                     /translation="LYSVFGEFPAWIAGWLTIMEFLTAVSSVASGWGAYLKGLLANFG
                     IAMPTALNGTFNPAAGTYVDLLPVLVLIFVVGVVLLNSKAALRFNSALVVLKFSALAL
                     FILVGLFFIKPENWSNFSPFGFGAIYGGQAGIMAGASLMFFAFLGFESISLAIDEVKK
                     PEKNVPKGIVLSLSIVTILYIVVTLVLTGMVHYTKLNVADAVAFALREVGLDWAASYI
                     SIVAILTLITVCISMTYALSRMVYSISRDGLLPKSLSRLTQTSKVPKNATILVGIFAA
                     ICAGIFPLASIASFLNICTLAYLIMLALGIIRLRQVEGLPKDGQFKTPLVPLLPILSI
                     IICLSFMFQYSLDTWLAFGVSVVIGILIYFCYGYRHSEAK"
     gene            1248..2129
                     /locus_tag="U1099_001184"
     CDS             1248..2129
                     /locus_tag="U1099_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM4867508.1"
                     /translation="MSNSDIRAKATVIREQTDGMITLFLAPVLISFLSGVAEFGLKQI
                     WGQTGTFSWGTTIVKDGVTWTHNFISVGPSIIFDFIAQYLIVTACFQLIRLVRKQRTK
                     VNFTECFTLLDGKNFLPVFSTTLLKMLVLYLASFPAALGMILIGLAFYSTIVIFGAPI
                     DYQPDFSAFFSSSYFQTGLILVCVGITCSLFVGYGLSQVSFLLYDYLENNTYSSPFKL
                     FSQSWQLMKGNKWRRFLLDLSFIGWFIGVLLTFGFLGLYVYPYYWTCQTIFYEDLKAK
                     NPLMFSTYKSVISSTVA"
     gene            2322..3158
                     /locus_tag="U1099_001185"
     CDS             2322..3158
                     /locus_tag="U1099_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM4867509.1"
                     /translation="MMTSDIVSRAMMTRERTRGTTLLFLPSILLSIVYAVPVTIFRLL
                     TFLELRQNSAVVEGRLVSSIRLTYLGELGFALLLQLFIGLSCFHLLELLRGDRQEFSL
                     KDSFSFLKTEWKKPICTTFLYYYLLLFMAGLPANLGAALFIKGFAMSSLMVLSPGAMA
                     ELAVHQPSQLMKIGAPIFLLGMIIYLFVYYTYSQVVFVLYDHLDNASYTGPLAVFRES
                     RFLMKGNYWQRIWLDLSFLGWFIGEVFTLHLLSFFVTPYYHLSRTIFYEKLKKQRLSA
                     VE"
     gene            complement(3176..4051)
                     /locus_tag="U1099_001186"
     CDS             complement(3176..4051)
                     /locus_tag="U1099_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4867510.1"
                     /translation="MKTEFINEKAQSAFNLNIITSPIVAIILLLVGDSLGAVIFTPIS
                     FLLPFSETLSVSFELFAFAFISLMVILWARYIEKSPWVGLGFTKKGAGKDFLLGWGIG
                     AAMLTTCTLIMWGLGAIEFTSIQFSTKLVGEFIILILAWSIQGTTEEILTRGWMFSSL
                     SAKHNIPIGIIVSSLFFTFLHLGNNAISLIPILDLTLFAVLACLIMLKTGNIWVIGGL
                     HAAWNCFQGNVFAFPVSGTDAGQAFVQIGITGPEWLSGGKFGVEGSIISLLVQGIMIS
                     WLVYDLYFKPKTSSL"
     gene            4174..4785
                     /locus_tag="U1099_001187"
     CDS             4174..4785
                     /locus_tag="U1099_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HEM4867511.1"
                     /translation="MELNQWLKSFQLKDNRLVGPFFYGTLLALRFEIGPADEAEELSR
                     AIYLERAYARAVELFEQASSEYDYVLLSLLRQEDRDIDTYLWHFSSKFNFDKVPEPEL
                     IEVEDWTGDVLVFECYLFPVTDQDLKALLREIVKADHGGFHYLSASVIFLSSKDKVLY
                     HCYDDRGVDIAVVDDDKRRQLFTDCHDLLFDYDMEEMVRRMDF"
     gene            4986..7746
                     /locus_tag="U1099_001188"
                     /pseudo
     CDS             4986..7746
                     /locus_tag="U1099_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            7743..9665
                     /locus_tag="U1099_001189"
     CDS             7743..9665
                     /locus_tag="U1099_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM4867512.1"
                     /translation="MSQLFEVTYGQKKTSVNTNELGMREMQARVCEKRNSPYLLVKAP
                     PASGKSRALMFVALDKLKRQGLKKAIVAVPEKSIGKSFRSTKLSDFGYYWDWIVKDEN
                     NLTTSGTAKSKVEKFINFIQSTDETDDVLIVTHATLRFASEQLDASDFDACLLAIDEF
                     HHVSQDDNSVLGNALREVLQKSTAHIFAMTGSYFRGDSVPILDHADEARFERVTYTYY
                     EQLEGYQYLKSFVMNYKFYRGRYTDAFPDLLPEFIDKKTIIHIPNVNSGESSKDKYGE
                     VDEILDCLGDYLHKDEHGIIHLRSKTGKILKIADLVTEDGRDLVSNYLAKMDSEDELD
                     MIIALGMAKEGFDWPFAEYALTIGYRSSLTEIVQIIGRVTRDSSNKTKAQFTNLIAEP
                     DAEDEEVQFSVNNIMKAITASLLMENVLSPSFKFKRKENDGQKANGAEIFIKGLAKPS
                     TERTQKIIEQDITDLKASVLQDDVVQAAISAGAESKVITKQLIPRVIATRYPDLTQTE
                     VEEVRQHLVADLTLSNAKKDSENNKLLKMADKFINIDDLSIDLIDSVNPFQGAYEVLS
                     RDVDSRTLSLIARVFDAKKVEFTTDELRMLYPQIKAFQAEHGHSPRRNSSDLEEERLY
                     FALAKLDELRRERDNQ"
     gene            9662..9877
                     /locus_tag="U1099_001190"
                     /pseudo
     CDS             9662..9877
                     /locus_tag="U1099_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9942..10820
                     /locus_tag="U1099_001191"
     CDS             9942..10820
                     /locus_tag="U1099_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM4867513.1"
                     /translation="MMDDLDDILNDDSLFENFETDRTLFSTKRYQGTVRARSKSSSRT
                     RVQTGFDTYRKLFTQVHADIACGKRQIKSIEETSQGNRISRKNPIKQGNFYVDNGVML
                     YVVKIYDPQTNVEVGQSTERKYKVHIVYENGTENHVWLLSLISSLYDKKRSGRLVTEP
                     LDTISMLGEEAAKYHTTGYIYVVKYAGTDNRFLELDNLYKIGVATDIKKRLSNSQNEA
                     TYLYAPVHLVASYEIQNVSASKLEKFLHDAFSDKRVQLETASPTGKKVSVTEWFVVEL
                     EQIEQKIYELFKQLNL"
     gene            11145..11891
                     /locus_tag="U1099_001192"
     CDS             11145..11891
                     /locus_tag="U1099_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867514.1"
                     /translation="MPYRKLTSRDRSFIKGQTDRLLNQMNSNAAYLGTVQTYEAAKAR
                     ANQAYLAMELEDDPEKKVRLAKLVENYTAQAQAILDEWEEKNSRTEKTTRNVKLILWL
                     TVLFTLSFVFPVNIILAILFSVWFYRNYLRSSRTDQVIKAANSQSLTEEERFEDWLTE
                     KIVVLNKDKRQWTEGIIAKYDSLDEIAAGIGEMDEIDKVAGEKLLRRYQDILADIEKL
                     PDSEQKTYYLDFHQEIIADLEDLFQQLHFG"
BASE COUNT         3571 a         2156 c         2638 g         3628 t
ORIGIN      
        1 ctctactcgg tttttggtga atttcctgcc tggattgctg gctggctgac gatcatggaa
       61 ttcttgactg ctgtatcgag tgttgcatct ggttggggag cttatttgaa aggactcctg
      121 gctaattttg gtatcgctat gccgacagct ttgaatggaa cttttaaccc cgctgcaggt
      181 acctatgtag acctcttacc agttctcgtc ttgatttttg tggtaggagt ggtcttgctt
      241 aattctaagg cagcacttcg tttcaattca gccttggtag ttttgaaatt ttcagcgttg
      301 gctttattta ttttagtagg gttattcttt atcaaaccag aaaactggtc gaacttctct
      361 ccttttggtt ttggtgccat ttatggtgga caggcaggaa ttatggcagg agcgtctctc
      421 atgttctttg ccttcctggg atttgagtct atttcattag caattgatga ggtgaaaaaa
      481 cctgagaaga atgtaccaaa gggaatcgtg ttgtctctat ccattgtaac aattctttat
      541 attgtagtga cattagtctt gactggtatg gttcactata ctaagttgaa tgtggcagat
      601 gcggtagcct ttgcacttcg agaagttgga ttggattggg cagcgagtta tatttctatt
      661 gttgcaatcc taacattgat tacagtatgt atttcgatga cctacgcctt gtcgagaatg
      721 gtctattcca tcagtcgtga tggtttgcta ccgaaatcat tgagccgatt gacacaaaca
      781 agcaaggttc ctaaaaatgc gaccattctt gtggggattt ttgcagccat ttgtgcaggt
      841 attttcccac tagcaagtat tgcttccttc ctcaatatct gtaccttagc ttatttgatt
      901 atgctggcgt tgggtattat tcgtctgcgt caagtcgaag gtttgccaaa agatggacaa
      961 tttaaaacac ctcttgttcc cttgttacct atcctctcga ttatcatttg tctttcattt
     1021 atgttccagt atagtttaga tacttggtta gcattcggag tatccgtggt tattggaatt
     1081 ttgatttact tctgttatgg ctatcgtcat tcggaagcaa aataatagat ttcccttctt
     1141 tatttgaagg gattttttgt acaatttttt tatagaaata atggaaaact taaaaataaa
     1201 atagtataat atatataatg atacaaatat atgaaaggag aaacgcaatg tctaatagcg
     1261 atattcgagc taaagcaacg gttattcgtg aacaaacaga tgggatgata accttgtttt
     1321 tggcacctgt tttgatttct tttctgtcag gagtggcaga atttggcttg aaacaaatct
     1381 gggggcaaac tggtactttc tcctggggga caactattgt gaaagatggt gttacttgga
     1441 cgcataattt tatttctgtg ggcccgtcaa tcatatttga ttttattgca caatatttaa
     1501 ttgtgacagc ttgttttcaa ctaataagac tggtgagaaa gcaaagaacc aaggttaact
     1561 ttacggagtg tttcacttta ctagatggga agaatttttt accagttttt agtacgacat
     1621 tattgaaaat gctggtcttg tatttagcaa gtttccctgc tgctcttgga atgatattga
     1681 taggtttagc tttctatagt actattgtca tctttggtgc tcctattgat tatcagcctg
     1741 atttttcagc ttttttcagt tcgtcttatt ttcaaacagg attaatactg gtatgtgtag
     1801 ggattacttg tagtttattc gtaggatatg ggctcagtca ggtctctttt cttctctatg
     1861 attatttgga aaacaatacc tattcttcac catttaaact cttcagtcag agctggcagt
     1921 tgatgaaggg gaataaatgg cgccgtttcc tcttggattt atcttttatc ggctggttta
     1981 ttggagttct tttgacgttt gggtttttgg gactttacgt atatccatac tattggactt
     2041 gtcagactat attttatgaa gatttgaagg ccaagaatcc attgatgttt tcaacatata
     2101 aatcagttat ctcttccaca gtagcctagg gagtatggag ataacaaatt attaggaatc
     2161 ccttcgtatt ttggagggat tttcctgttt ttaagagatg ttctggaaaa ttaatggata
     2221 aagttttata ctaatagtag gatggtaaag tagtccgatg ttgatggaat atttgcgacg
     2281 tgacttcata aataaggaga gaaaattgga ggggaattgt gatgatgacg agtgatatag
     2341 tatcaagagc gatgatgact cgcgagcgga caagaggtac aacactacta tttttgccct
     2401 ccattttgct atctattgtt tatgctgtac cagtaacaat ttttcgccta ctaacatttc
     2461 tagagctccg tcaaaacagt gctgttgtgg aagggcgatt ggtttcttca atcaggttga
     2521 cttatttagg tgagttaggc ttcgctctgc tgttacagct gttcatcggt ctgtcctgtt
     2581 ttcatttatt ggaattgttg agaggggata gacaggagtt ttctttaaaa gatagttttt
     2641 cctttctaaa gacagaatgg aagaagccta tctgtaccac ttttctttat tattatttgc
     2701 ttctttttat ggctggactt ccagctaatc taggggctgc tttatttatt aaaggcttcg
     2761 caatgagtag tttgatggtc ttatctccgg gagcaatggc agagctggca gttcatcagc
     2821 ctagtcaatt aatgaagata ggggctccga ttttcctgct aggaatgatt atttaccttt
     2881 ttgtctacta tacctactca caggttgttt ttgtcttgta tgaccatttg gataatgcta
     2941 gttatactgg tcccctggct gtatttaggg aaagtcgttt cttgatgaaa gggaactatt
     3001 ggcaacgtat ttggttagat ttatcctttt taggatggtt tatcggggaa gtatttaccc
     3061 ttcatctgtt atcttttttt gtgactcctt actatcattt atccaggacc attttctacg
     3121 aaaaattgaa aaaacaaaga ctgtcagctg tagagtagct aacagtcttt tatggttata
     3181 gggagcttgt tttgggcttg aaatagagat cgtaaaccaa ccaggaaatc attattcctt
     3241 ggacaagcaa gctgatgatg gagccctcta cgccaaactt tccaccagat agccactcag
     3301 gaccagtgat accgatttga acaaaggcct gaccagcatc ggtgccgctg actgggaaag
     3361 caaaaacatt cccttggaaa cagttccacg cagcgtgtaa tccaccaatc acccaaatgt
     3421 tgcctgtttt gagcataatg aggcaggcaa gaacggcaaa tagggttaaa tccaaaattg
     3481 gaatgagtga aatagcattg tttccgaggt ggagaaatgt gaagaagagg gaagaaacaa
     3541 tgataccgat aggaatattg tgtttagctg aaagtgatga gaacatccat cctcttgtta
     3601 gaatttcttc tgtcgtacct tggatagacc aggcaaggat aaggataata aattctccga
     3661 ctaattttgt agagaactgg atgcttgtaa attcaatcgc gcctaatccc cacatgatga
     3721 gtgtacaagt ggtcagcatt gcagctccga ttccccaccc gagtagaaaa tcttttcccg
     3781 cacctttttt agtaaaacca agtcctaccc acgggctttt ttcaatatac cgtgcccata
     3841 aaatgaccat caggctgata aaagcgaaag cgaatagttc aaaacttaca gaaagggtct
     3901 cgctaaatgg gagtaaaaag cttattggag tgaaaataac tgctcctagt gaatctccta
     3961 ctagcaacaa aataatagct acaataggag aggtgatgat gttgagattg aaagctgatt
     4021 gagccttctc attgataaat tcggttttca taggacctcc ttttactaga tactttacta
     4081 gttcactatt ctatcatatt tttccaaaga agactagacc taatttccta gtatatggta
     4141 gaatagtaca aagctatata aggagaatag tatatggaat tgaatcaatg gctgaagtcc
     4201 tttcaactga aagataatcg actagtgggc ccattctttt atggcacact tcttgctctt
     4261 agatttgaaa tcgggcctgc agacgaagca gaagaattat caagagcaat ctatcttgaa
     4321 agagcctatg cacgtgcagt agaacttttt gagcaggcta gttcagaata cgactatgtg
     4381 ctcctttcgc tccttcgaca ggaagatagg gatatagaca cctacctctg gcatttttca
     4441 tcgaaattta acttcgacaa ggtccctgag cctgagttga tagaagtgga ggattggacg
     4501 ggagatgtgc tagtctttga gtgctatctc tttccagtta ccgaccagga cctgaaggct
     4561 ttgttgaggg agattgtcaa agccgaccat ggaggttttc actacctttc tgcttcagtt
     4621 atctttctat ctagcaagga caaggttctg tatcattgct atgacgatag gggagtggat
     4681 atcgcggtgg tagatgatga caaacgacgt cagcttttta cggactgtca tgacctgctt
     4741 tttgactatg atatggagga gatggtgagg aggatggatt tttaaaaaaa ctaatataaa
     4801 aatgcccatg atatactacg tataccatgg gtttgacttt cagtcaaacc ctaacaacaa
     4861 cagtcctatc acaaaataag attaggaaac tgaatggtgt gtttagtata ataacgtggg
     4921 ttattttttc atcaaacgct ccttttgtac taatacaaat gtgttaggaa aagctaggag
     4981 atcttatggc aaagtttgat attattacaa tcgaagacaa gattcagcag ttattggagg
     5041 atggttttga ccaagcttct tttatctatg aattcttgtc tttttatgat atttcaaaaa
     5101 cagctatcac ccgaatccgt aaagaacaaa gtggtgaaat caagaacaaa ttgtattaca
     5161 agacggttcc tactgataag tcggttgtgg caggtgttgc tgagattgaa gaagcaatca
     5221 aggataaaaa atcacaaccc cgttatatca ttgtgaccaa ctttgtggac tttgcagcca
     5281 aggataccaa gactttcgat accttacaga tcccttttaa ggatttgccg acctatgccg
     5341 atttcttctt ggcttggaat ggtgtagaga aggtagatta tgccaaggaa aatcctgcgg
     5401 atattaaggc tgcggaacgg ttcacacgtt tgtatgatga attaaagaaa atcaatcact
     5461 ttgatgaccg tagtcaagag gaaaaagagt ttaacctgtt tttgattcgc ttactttttc
     5521 ttttatttgc cgaagataca gaaatcatga aaaaaggtat ttttaccaat gccatcaaaa
     5581 ctcgctcgcg agaggacggc agtgatctga atactctttt taatcagata tttgccagtc
     5641 tggatattgc taatcgtctt agccaagaaa actggttaca ggaatttcca tatgtcaatg
     5701 gaaaactttt tacagatgca tcaaccgatc tagtttttga taagaaaaca agaaaattaa
     5761 tcttggaagc aggcgagctc ctcaactgga atgaaatcaa tccggatatt ttaggggcca
     5821 tgattcagac agtggctagc agtgagcacc gttcaagctc tggtatgcac tatacttcgg
     5881 ttgaaaacat catgaaagtg attaaaccgc tctttcttga tgaactgact gcaaatttcc
     5941 agactattgt agataaaatc aatgagaacg aagacaagga cataaccgag cgaaccaggc
     6001 gagagaatag ggcggcttac gaaaaagaat tgtccacact tcacgaacac ctatccaaga
     6061 taaaattctt tgacccagcc tgtggctcag ggaatttcct tatcatcacc tataaagagc
     6121 ttcgtcgttt ggaaataaga ctattaaaag cactacgaga actacgacaa gatggaaaaa
     6181 ttgatttctt cgaaacgtcg aagattagct tgaatcagtt ttatggaatt gaattagatg
     6241 attttgcaca tgaagtagct agattatctc tgtggattgc tgaatatcaa atgaatttag
     6301 aagcactaaa tgaaatcaat ttacaatctg cctttctccc tctgcgtgat gctggaaaca
     6361 tcactcaagg aaatgccctg agacttgact gggataaagt tgtccctcat accccaaccg
     6421 atgaaatata cctgatagga aatccgcctt atattggggc aaaaattgca aaataaagaa
     6481 cagaaagaag atttacgcct gacgattggt gaacaattca agtataagaa aatggactac
     6541 attgcaggct ggttctacaa agggattgat ttcataaata cttcgaatag caaacttgct
     6601 tttgtaacaa ccaactctat ttttcaaggg gaacaagttg cttttatttg gcaacctgta
     6661 ttatataaaa tggagataga gtttgcttat acatcattca agtgggggaa cagcgctgca
     6721 aataatgctg gggtaactgt tgctatagtt ggattggcaa ataagaacaa tgcaagttac
     6781 caaaaatata tttttgatga aaccttggta cgtcaaacgg ttactcatat caatccctac
     6841 ttagctagcg gggaagatat actggttgag agtagtaata gtccgattaa tagattgcct
     6901 aaaatggtgt tcggtagtat gcctagagat ggcggtcatc tgattatttc tgacttaaat
     6961 gaaaggaata agatagtaga aggaaaccct gatcttgagc cgtttattaa gagatatatc
     7021 ggcagtgaag agtatatcaa agataaacca cgatatgtgt tttggctgaa taaagaagaa
     7081 tatcaaactg ttcagaataa ctcattgtta ggtttaagga ttaagggtgt agcgcaggct
     7141 aggatgagta gccctgcaaa gtccactcaa gatgcagcaa aaacacctta tgcatttgtt
     7201 caaaaaggtg agtgggacga agcttactcg accttcaaac aatcaggtgc agatgagttt
     7261 gttcagctct tggttcctcg agtatcatct gaaagtcgtg actatgttcc tatgggattt
     7321 gttggagaag ataccatcat ttctgattct gccatggctg tctacaacgc ccctgtctat
     7381 ctgcttggtc ttctcatgtc acggcttcac atgacctggt tgcgggctat cggtgggaag
     7441 ttagaaactc gttatcgata ctctgctggt ctggtttaca acaccttccc tgtaccagag
     7501 ttatccacta agcgaaaaaa tcagattgaa gaacaagttt ttgaaatcct tgacttacgg
     7561 gaagaattag gtggtacctt agcagagctt tatcatcgtg ataccatgcc acaagcactc
     7621 cgagaagccc atcaggtact agatagcatt gttgaacgcg cctataagga aacaccattt
     7681 accagcgatg aagaacgcct cagccacctc ttgaaacgct ataagaaaat gaaggagcaa
     7741 tcatgagcca gctttttgaa gtgacctatg gtcagaagaa aaccagcgtc aataccaatg
     7801 aattgggcat gcgtgagatg caggcgcgtg tctgtgaaaa gagaaacagc ccctacctct
     7861 tggtcaaggc cccgccagca tccggtaagt ctagggcact gatgtttgta gccttggata
     7921 agttgaaacg ccagggcttg aaaaaagcta tcgtagccgt accagaaaag tccatcggca
     7981 agtcttttcg ttcgaccaaa ctttcggact ttggctatta ctgggattgg atagtcaaag
     8041 atgagaacaa cttaaccaca tcaggtaccg ccaaaagcaa ggtagaaaaa tttatcaact
     8101 ttatccaatc tactgatgag acagatgatg tcttgatcgt aacccatgcc acgctccgct
     8161 ttgcttcgga gcaactggat gccagtgatt ttgatgcttg tttgcttgcc attgacgagt
     8221 ttcatcatgt ctcccaggat gataattctg tattgggcaa tgccctgcgg gaagtcttgc
     8281 aaaaatcaac ggcccatatc tttgccatga ccggttccta tttccgtgga gatagtgtac
     8341 ctatcttgga tcatgcagat gaggcacgtt ttgagcgtgt gacctatact tactatgaac
     8401 agttggaagg ctatcaatac ctcaagtcct tcgtcatgaa ctacaagttt taccgtggtc
     8461 gctacacgga tgcctttcca gacttattgc ctgagtttat cgacaagaaa accattattc
     8521 atattcccaa tgttaattca ggtgagtctt caaaggataa gtatggagag gtggatgaga
     8581 ttttagattg tctaggagat tacctgcata aagatgagca tgggattatt catcttcgtt
     8641 ctaagacagg taagatcttg aagattgcgg acttggtgac tgaggacggt cgtgatttag
     8701 tcagcaatta tctggcgaaa atggatagtg aagacgagct ggatatgatt attgctcttg
     8761 ggatggctaa ggaaggtttc gactggccat ttgcagaata tgccctgact atcggctacc
     8821 gctcttcgct gacagagatt gtccagatta tcggacgggt cacgagggac agctcaaaca
     8881 agaccaaagc ccaattcacc aatctaattg cagaaccaga tgccgaagat gaggaggtcc
     8941 aattctctgt aaataacatc atgaaagcta ttactgctag cttgttgatg gaaaatgttt
     9001 tatctccttc attcaagttt aagcgtaagg aaaatgacgg ccagaaagct aacggagctg
     9061 agatttttat caagggtcta gcaaagccat caacggaacg tactcaaaaa atcattgagc
     9121 aagatataac tgacttaaaa gcctctgtct tgcaagatga tgtggtacag gcagctattt
     9181 ctgcaggtgc agagagcaag gtcatcacca aacagttgat tccaagagtg attgcgactc
     9241 gttatccaga tttgacacag acagaggttg aagaggttcg tcagcattta gtcgctgatt
     9301 tgaccttatc caatgctaag aaagacagcg aaaataataa gctgttaaaa atggcggata
     9361 aatttatcaa tattgacgac ttaagtatag atttgattga tagtgtcaat ccatttcaag
     9421 gtgcttatga ggttctgtcc agggatgtag atagtcgtac cttaagcctg attgcacggg
     9481 tatttgatgc caaaaaagta gagtttacaa ccgacgaatt gcgcatgctt tatccccaga
     9541 ttaaggcttt tcaggctgag catggacatt caccaagacg aaattcctca gatttagagg
     9601 aagaacgact ttactttgcc ttagctaagc ttgatgaact ccgtcgtgaa agggataatc
     9661 aatgatagaa aattttgcaa ccttagaaga tatttttgcg gatgaagcct ttgaaagctt
     9721 ggttgctggg attcgtgttg tcaaagttga aagattagac ccagaaatag agaaatttat
     9781 ggaaatctgt caatgggtta aggaacatgg acgagagccg caaagaagta ctcaaatcaa
     9841 agaacgacaa ttattcagtc gactgaaggc tattcgtgca gatgagggga gaagagctca
     9901 agttagcgcc tatgatgaat tggatttgtt aggagataga catgatggat gacttagatg
     9961 atattttaaa tgatgattcc ttgtttgaaa atttcgaaac agatagaact ttatttagca
    10021 ctaaacgcta tcagggaaca gttagagcta ggagtaaatc tagtagtcgt acacgtgttc
    10081 aaactggctt tgacacctat cggaaattgt ttacacaggt tcatgctgat attgcttgtg
    10141 gtaaacgtca gataaaatcc atcgaggaaa ccagtcaagg aaaccgtatc agtcggaaaa
    10201 atccaattaa acagggaaat ttctatgttg ataatggtgt tatgctgtat gttgtcaaaa
    10261 tttatgaccc tcagaccaat gtggaagttg gccagtctac agagcgtaag tacaaggtgc
    10321 atatcgtata tgaaaatgga acggagaatc atgtttggct cttgagtttg atttcatcac
    10381 tatatgataa aaaacgttcg ggtagattgg taacagaacc cttggacaca atttctatgc
    10441 taggtgagga agccgcaaaa tatcacacaa caggctacat ttatgttgtc aaatacgctg
    10501 gaacagataa tcgattttta gaattggata acctctacaa gataggggta gctacagaca
    10561 tcaaaaaacg gctttcaaat agccaaaacg aagcgactta cctgtatgct ccagtccatc
    10621 tggtagccag ttatgagatc cagaatgttt ccgcaagtaa gttagagaaa tttctacatg
    10681 atgcatttag tgataaacgg gttcaactgg agactgcctc accaactggc aaaaaagttt
    10741 ctgttactga gtggtttgtg gtagaattag aacagattga acaaaaaatt tatgagcttt
    10801 ttaagcaact aaatctctga catcttatca gctaaaccag tggagaaata tattgttcgt
    10861 ttctattttc acatttgcga tttgttatct gctaggcatt atcgccatag ccatagcttt
    10921 cacattcttt cgcaggaagt agaaggacgg acagatggac aaacttgtta tttggttttt
    10981 gattttactc tgggtttcac cagtgattat tgttgccttg gcaggtggat tcattatttt
    11041 cagtctctta tttggtagac ggaaataatg tctttaatca ttgcaaattc acacaaccta
    11101 tcgtaaaata gtaatatatc taggatatac aggaaggggt tgctatgcct taccgtaaat
    11161 taacatcaag agatcggtct tttatcaaag ggcagacgga ccgtttgctg aatcagatga
    11221 atagcaatgc ggcctatttg gggacagttc agacctatga agcagccaag gctagggcca
    11281 accaagccta tctggcaatg gaactggaag acgacccaga gaaaaaggtg cgactggcaa
    11341 aattggttga aaactatact gctcaagccc aggctatatt ggatgagtgg gaggagaaaa
    11401 acagtcgtac agagaaaacg accagaaatg tcaagttgat cttgtggctg accgtactgt
    11461 tcacccttag ctttgtcttc cccgtcaata tcattctagc cattcttttt tccgtttggt
    11521 tttatcggaa ttaccttcgt tcaagtagaa ctgaccaagt gattaaggct gctaatagcc
    11581 aatctttgac agaagaggaa aggtttgaag actggttgac agaaaagatt gtcgtcctca
    11641 acaaggacaa gcgccagtgg acagagggga taatcgcaaa atatgacagt ttagatgaaa
    11701 ttgcagcagg tatcggtgaa atggatgaga tagataaagt agctggtgag aagcttcttc
    11761 gacgctatca ggacatttta gcagatattg aaaaactgcc agattctgaa cagaaaacct
    11821 attatttgga ttttcatcag gaaatcattg ccgacttgga agatctattc caacaactcc
    11881 attttggata agaaaaaccg gtaacagtac aaggctgttg ccggttttgt tatgtgactt
    11941 ttagtccgtc gcgctccgta aggtgcgcga cgaaggttat accaaaaaaa ctc
//
LOCUS       DASFSW010000061        11865 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.061, whole genome shotgun sequence.
ACCESSION   DASFSW010000061 DASFSW010000000
VERSION     DASFSW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11865
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.061"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..2192)
                     /gene="ide"
                     /locus_tag="U1099_001193"
     CDS             complement(<1..2192)
                     /gene="ide"
                     /locus_tag="U1099_001193"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS family IgM protease"
                     /protein_id="HEM4867515.1"
                     /translation="MNIQERFSLRKSAVGLVSVSLLCAIYTSTVAADTVVTGVNEIIE
                     ESQVKDEVSIESEKNESLDGSNIEIVEEIADNIPSPVIAEGEVAVEMKVDRGTENVVS
                     RNDTEVTTSEQNQIEVTETKEILNQTSYQTESGEQRQIIWAHGITPPAMEQSGGFVKE
                     KYGDYLNYTAPFKAGKGYYDTNKSLNASFIDLNLCFAAVSSNMVHWWLEQNSSYVERY
                     LKEKKGTVNVGENYAITDLRRYIDSFQDQQNSRVFDMFKTYYGYRTNGFVSDALVDLF
                     INGYKPKVQGGVNLEDSQLVPDSRGGFFYDVFKEKKLTNRIFSGSYERFGEDVRTVLE
                     SKGLLGLTYRTLGYATHIVTVWGAEYDNQGKIRAVYITDSDDQQEQIGLKRMGITRDA
                     SGNPRLNNHVKNNSAGALLDYVHTIRLGQDLWEEYFNPFAKAKEIASQILADRKKALV
                     LSIQGQSELPESMRLIYLEKLNNLYNQGILSIQKTESSEMLSGALENGLNSLKSLDFP
                     ISEVGNALAPDLSVGDRSTVSDVDSLSSQETSSTNLEADTENAGIIADGTNQLHFPVE
                     AQTTSSVEAEGDNVFEQEADTLPIIIENKDEFGSELSGNMQTSETDSLVVAVEEDVKN
                     DEVDQVEKLLESEKVENQSSELLSDTLIVEGANDKEEDRVEAVVSEQPDSIPHQNVEI
                     SPVEPMNVETESVVTPINDAATPHGFPMYIDNSVTESVAIPLEKDSIQA"
     gene            complement(2353..3858)
                     /gene="pyk"
                     /locus_tag="U1099_001194"
     CDS             complement(2353..3858)
                     /gene="pyk"
                     /locus_tag="U1099_001194"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEM4867516.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGDDGYWGEKLDVESSAQKIAA
                     LITEGANVFRFNFSHGDHQEQGDRMATVRRAEEIAGKKVGFLLDTKGPEIRTELFEDD
                     AKEYAYTTGDKLRVATKQGIKSTKEVIALNVAGGLDVFDDVEVGKQILVDDGKLGLTV
                     VEKDAVNREFVVLVENDGVIAKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEVVRNICKETGNDHVQLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIAKNAQTLLNEYGRLNSDQFNRASKTEVIASAVK
                     DACHSMDIKLVVTVTETGYSARSISKYRPDADILAVTFTEKVQKSLMLNWGVIPIVTN
                     RPDSTDDMFDLAERIAKEQGLVEAGDDIIIVAGVPVGVAGSTNTMRVRTVK"
     gene            complement(3925..4935)
                     /gene="pfkA"
                     /locus_tag="U1099_001195"
     CDS             complement(3925..4935)
                     /gene="pfkA"
                     /locus_tag="U1099_001195"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEM4867517.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVYGINEGYAGM
                     VAGDIHELSARSVGDIISRGGTFLGSARYPEFAKLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGVPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRHAGDIALWAGIASGADVIVVPEEDFNINDIVDRIKAGYDNGKKHSII
                     VLAEGVMPAAQFAEELKAAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGRGGLAVGIRNEQMVENPILGTAEEGALFSLTTDGKIVVNNPHKADLELADLNRNL
                     SI"
     gene            complement(5020..8130)
                     /locus_tag="U1099_001196"
     CDS             complement(5020..8130)
                     /locus_tag="U1099_001196"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEM4867518.1"
                     /translation="MLAQLDTKTVYTFMDSMVTIENYVGRAKELGYHALGIMDKNSLY
                     AAYSFMESCNKMGLQPIIGCELDWKLQEGEQDVTTQLIALTTRGYRNLLKISTAKMIG
                     QDDFETIRQYLVDIAVVVPYFSGIEHLELGVNYYIGVTVDTPVQDFKQAILPIHTVRF
                     FEEEQLESLQVLHAIRDNIPLNQVVDIDSRQKLLSADSLTNLFSNRFPQAVDNLRDLV
                     AGISYQLNTDLKLPRFNRDRPAVEELYERSYQGLEEKGLTNSLYKERLEKELSIIHQM
                     GFDDYFLIVWDLLRFGRSQGYYMGMGRGSAVGSLVAYALNITGIDPVKHNLLFERFLN
                     AERYSMPDIDIDIPDIHRADFIRYVRDRYGSTHAAQIVTYSTFGAKQAIRDVFKRFGT
                     PEYELSNITKKISFRDSLTSAYQRNAAFRQIINSKIEYQKAFAIAQQIEGQPRQTSIH
                     AAGIVMSDEDLTDTIPLKIGDDMLVTQYDAHAVEANGLLKMDFLGLRNLTFVQKMAEG
                     VRERYQQEIDIAKIDLEDKKTLELFAAGRTKGIFQFEQPGAISLLRRVKPGRFEDIVA
                     TTSLNRPGASDYSDNFVKRKHGQETVDLLDPTIAPILQPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNAQEMQVMEAEFLQGALKNGHAEENAREIFGMVAKFAGYGF
                     NRSHAYAYSALAFQMAYFKSHYTDVFFDVMLNHSSGAYIEDAMQFDFEVARLTINNIP
                     YHDKFDKSKVYLGLKTLKGLPKDLALWILDHRPYKSVEDFILRLPDNFKKKDLLIPLI
                     QIGLFDDFVANRKKIIENLDNLFVFAEAIGTFFAEESYSWTDVEDYSDSEKFMLEQEL
                     LGVGISPHPLVNICRESKRSFTDLADLAVGNRATVLVQIQAIRLIRTKKTGEQMAFLR
                     VTDTKRTLDVTLFPESYRQYASILEEGGMGYITGRVQERDGQLQLVLEKLEPVSLEKF
                     WILLENREHDYAVARILDKYRGSIPVVLHYQDSNQTIQAEHHMVMKTPQLEEELSEFV
                     MKTIFR"
     gene            8288..8662
                     /locus_tag="U1099_001197"
     CDS             8288..8662
                     /locus_tag="U1099_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM4867519.1"
                     /translation="MAWKFDNNIPIYIQISNTIKLQIVTNQLKSGDKLPTVRDLADIA
                     GVNPNTVQRALSDLETEGFVYSVRTTGRFVTDNQELITQTRLHLAQKELENFVNNMLE
                     LGFRHDELTSQLDDYLKGVTYE"
     gene            8655..9362
                     /locus_tag="U1099_001198"
     CDS             8655..9362
                     /locus_tag="U1099_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867520.1"
                     /translation="MSNAYTLVELQQVSKSYGGAVALNNVNLKLTAGKIIGLLGPNGS
                     GKTTMIKLINGLLQPEYGQVLINGRTPSPETKAIVSYLPDTTYLDEHMKVLEAITFFE
                     DFYADFDKERALHLLQDLGIDLNSRMKHLSKGNKEKVQLILVMSRQAQLYVLDEPIGG
                     VDPAARDYILKTIVNNYSPTASVIISTHLISDVEQILDEVIFLHYGSVIRHGNVDDLR
                     IESGESIDELFRQDFKA"
     gene            9379..10161
                     /locus_tag="U1099_001199"
     CDS             9379..10161
                     /locus_tag="U1099_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867521.1"
                     /translation="MFKKLLKYEFQSVGKWYLGIYIAAVVLSAVLGFWLQALALRTQA
                     GNTEPGGGEMVLLGTSFMTFGILIAALFLSTFVLVINRFRKNVYGRQGYLTMTLPVSS
                     YQIILSKLLASIVWYILAGITCLLSIGIILAVILAASEEAIMPEIQTFVQAIDWTVLF
                     SQIFYYLIEITTGILLIYFSISVGQLFKDHRFLFAILTYIGILIVTGVFSTFFVLSNL
                     GTAYSTPFSLYPSPVLALVNIILAFAYYFGTHFIMTKKLNLQ"
     gene            complement(10205..11206)
                     /locus_tag="U1099_001200"
     CDS             complement(10205..11206)
                     /locus_tag="U1099_001200"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEM4867522.1"
                     /translation="MLNVNELVKTELHCHLDGSLSLGVIRQLAQMAKITIPAEDEALR
                     KLVSVHGKVDSLMAYLKLFDFVRPLLQTATALELAAYDLVRQAASDKVIYIEVRFAPE
                     LSTDQDLTILEAVSAVLVGLNRGQEDFGVLAKLLVCGLKQTNTNQTKELFSAIADLAP
                     KGLVGFDFAGNEADYPTEELRDIIQFTQSLGYPMTFHAGECGCVINVIQALELGIRRI
                     GHGTALTRNPEAIQAFVNSGATLEMCLTSNLQTGAADSIEYFPYRELVEAGANITINT
                     DNRTVSNTTLNREYQLFVEYFGTSKADFYRFNQNAIQASFASEGEKKVLLELLDQQY"
     gene            complement(11365..11452)
                     /locus_tag="U1099_001201"
     tRNA            complement(11365..11452)
                     /locus_tag="U1099_001201"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11416..11418),aa:Ser,seq:gga)
     gene            complement(11455..11802)
                     /gene="ssrA"
                     /locus_tag="U1099_001202"
     tmRNA           complement(11455..11802)
                     /gene="ssrA"
                     /locus_tag="U1099_001202"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3479 a         2641 c         2019 g         3726 t
ORIGIN      
        1 gcttgaatgg agtctttttc aagtggaata gctacagatt cagttacgga attatctata
       61 tacatcggga aaccatgagg agtagctgca tcattaatag gagtgaccac agattcagtt
      121 tcgacattca ttggttctac aggagagatt tctacatttt gatgtggtat tgagtctggt
      181 tgttcagaaa caaccgcctc cactctatct tcttctttgt catttgcacc ctctacgatt
      241 agggtgtctg acagaagttc cgaactctga ttttcaactt tttctgattc aagaagcttt
      301 tcaacttggt ctacctcatc atttttcaca tcttcttcaa cagctactac tagcgaatcc
      361 gtttctgacg tttgcatgtt tcctgatagt tctgaaccaa attcatcctt gttttcaata
      421 attattggta atgtatctgc ctcttgttca aaaacattat ctccctcagc ctctactgaa
      481 gatgtcgttt gggcctccac tggaaaatgc aattgattgg taccatctgc aataatacct
      541 gcattctctg tgtctgcttc caaatttgtg gaacttgttt cttgagatga tagagaatca
      601 acatctgaaa ccgttgagcg atcacctact gataaatctg gtgccaaagc atttccaact
      661 tctgaaatag gaaaatctaa actctttaaa ctatttaaac cattttccaa tgcaccactt
      721 agcatctcag aactttctgt cttttgaata gatagaatcc cttgattata gagattattt
      781 agtttttcaa gataaatcag ccgcattgat tctggcaatt cagattgtcc ttgaatagac
      841 agaaccaacg ccttctttct atcagctagt atctgactag ctatttcttt ggcttttgcg
      901 aacggattga aatattcttc ccataagtct tgaccaagac ggattgtatg gacataatcc
      961 aaaagcgccc cagctgaatt atttttcaca tgattattca aacgcggatt tccagaagca
     1021 tcacgagtga ttcccatacg cttcaaacca atttgttctt gttgatcatc ggaatcagtg
     1081 atatagacag ccctaatctt accttgatta tcgtactcag caccccatac cgtcacaata
     1141 tgcgttgcat agcctaatgt tctataagtt agaccgagta atcctttgct ctccaaaaca
     1201 gttcgaacat cctcaccaaa acgctcatag ctaccactaa aaatacgatt tgtcagtttt
     1261 ttctctttga aaacgtcgta gaaaaagcca cccctactat ctggtactaa ctggctatct
     1321 tccagattga caccaccctg taccttaggt ttatatccat taataaacaa gtcaactagg
     1381 gcatctgaca caaaaccatt tgtacgataa ccgtagtaag ttttgaacat atcaaagact
     1441 cgactatttt gctgatcctg gaacgaatca atatagcgac gtaggtccgt tattgcatag
     1501 ttttccccaa catttactgt accctttttt tctttgagat atcgctcaac ataggaacta
     1561 ttctgttcca accaccaatg taccatgttg gaagacacgg ctgcaaaaca taggttaagg
     1621 tcaataaatg aagcattgag acttttattg gtatcataat agccttttcc tgccttaaat
     1681 ggcgctgtat agtttaaata gtctccatac ttttccttta caaaaccacc gctttgttcc
     1741 attgcaggag gagtaattcc atgggcccat ataatttgtc gttgctcgcc actctccgtt
     1801 tgataactgg tctgattcaa aatttctttt gtctcagtaa cctctatctg attttgctcg
     1861 ctcgtcgtaa cttctgtatc atttctagaa actacattct cggtccctct gtcaactttc
     1921 atctctaccg ctacttcccc ttcagcgata acaggtgatg ggatgttgtc tgctatttcc
     1981 tctacaattt caatattaga accatctagg gattcatttt tttctgattc aatagatacc
     2041 tcatccttga cttgtgattc ttcaattatt tcattcactc ctgtaacaac tgtatcggca
     2101 gcaacagtgg atgtataaat agcacatagc aaagagactg agaccaagcc aaccgcggat
     2161 tttctcaaag aaaatcgttc ttgaatgttc atctttcctc cgtttatatt tatattcttt
     2221 gaacaagtta ttcttactct tatttatttt ttatatcaac atcccaaaat catttagaga
     2281 caaaaaatct ataacagcac gtatttaact tattctctaa ttactacaaa aaggtccaag
     2341 cggacctttt tattatttta ctgtacgaac acgcatagtg tttgttgaac ctgcaacacc
     2401 tacaggcaca ccagcaacga taataatgtc atctcctgct tcaacaagac cttgctcttt
     2461 ggcaatacgt tcagccaaat caaacatatc gtcagttgaa tctggacggt ttgttacaat
     2521 tggaataaca ccccagttaa gcatcaatga tttttgtact ttctcagtaa atgttactgc
     2581 caagatgtcc gcatctggac ggtatttaga gatcgaacga gctgagtaac ctgtttctgt
     2641 aactgttaca actagtttga tgtccattga gtgacatgcg tcttttactg cagaagcaat
     2701 aacttctgtt ttagaggcac gattaaactg atcagagttc aaacgaccat attcattaag
     2761 aagagtctgt gcgtttttag cgatagttgc cattgtacga actgattcta ctggatattt
     2821 accgtttgca gactcacccg aaagcattgt tgcgtcagta ccgtcgataa ctgcgttgaa
     2881 tacgtcagat acttcagaac gagttgcacg tggtttttca gtcattgttt ccaacatgtt
     2941 tgttgctgtg ataactgctt taccagctgc attcacttta gtgataatca ttttttggta
     3001 aactggaacc atttcgaatg gtacttcgat acccatgtca ccacgagcaa tcatgatacc
     3061 atctgctgct tcgatgattt cgtcgatgtt gtcaatacct tgttggtttt cgattttcgc
     3121 aaacaattga acgtggtcgt taccagtttc tttacagatg ttacgaacaa cttctacgtc
     3181 ttttgcagta cgtacaaatg agatagcaat aaagttcaaa ccttgctcaa gaccgaagcg
     3241 gatatctgca ttatcacgct ctgcaagagc tgggaaagga atttttgtgt aagggatgtt
     3301 tacacctttt tgtttagcaa ttacaccgtc gttttcaacc aaaacaacaa attcacggtt
     3361 tactgcatct ttttcaacaa ctgtcaaacc aagtttacca tcgtcaacaa ggatttgctt
     3421 accaacttca acatcgtcaa agacatccaa accacctgct acgttcaagg caatcacttc
     3481 ttttgtagat ttgatacctt gttttgtagc aacacgcaat ttgtcaccag ttgtatatgc
     3541 atattctttt gcatcatctt caaacaattc tgtacggatt tctggacctt ttgtatccaa
     3601 caagaaacct acttttttac cagcgatttc ttctgcacga cgaactgttg ccatacgatc
     3661 accttgctct tggtggtcac cgtgtgagaa gttgaaacgg aaaacattgg caccttctgt
     3721 aatcaaagca gcaattttct gtgctgatga ctcaacatct aatttttcac cccagtaacc
     3781 atcgtcaccg aattttttac caccgcggat ttctaccgct ggaccaaggg ttgcaacaat
     3841 tttaacacgt ttgttcatct taacaaaact ccttttattt caatggtcat acgaccctta
     3901 attaacactt ttaaacaacg atacctagat tgacaagtta cggttcaaat cagccaactc
     3961 caaatctgct ttatgcggat tattaactac aatcttgcca tcagtagtca agctaaacaa
     4021 ggcaccttct tctgcagttc caagaattgg attttcaacc atttgctcgt tacggatacc
     4081 tactgcaaga cctccacggc cttctttaag caatttaacg gcatgagcac ccatacgaga
     4141 agcgagtaca cgatcacgcg ctgttggaga accaccacgt tggatatgac caagttctgt
     4201 cacacgaaga tcacttgtat cgcctgctgc tttcaattct tcagcgaact gtgcagctgg
     4261 cataacacct tcagccaaaa caatgatgct atgtttctta ccattgtcgt agcccgcttt
     4321 aatacggtca acaatatcgt tgatattgaa gtcttcttct ggaacaacga taacatctgc
     4381 accagaagca ataccagccc aaagagcgat gtctcctgca tgacgtccca ttacttcaat
     4441 gacgaaagta cgacggtgac ttgatgaagt atcgcgaatc ttatcaatcg catccatggc
     4501 agttgttaca gctgtgtcaa aaccgattgt aaaatctgtc cccacgatgt cgttgtcaat
     4561 ggtaccaggt acaccaatag ctgggaaacc atgctccgtc aagcgcattg ctccgtggta
     4621 agaaccgtca ccaccgataa ccactacacc ttcgatacca tgttttttca attgctcaat
     4681 ccctttaagt tgcccttcta atttggcaaa ttctggataa cgagctgaac caaggaatgt
     4741 tcctccacgt gaaataatat cacctacaga acgtgcagac aattcatgga tatcacccgc
     4801 aaccatacct gcgtaacctt cgttgattcc ataaacttcc attccttctg aaattgcttg
     4861 gcgaacaact gcacgaatag cagcgttcat accaggggca tcaccaccac tggtcaaaac
     4921 agcaatacgt ttcattcgtt aatgctcctt taacttttca ttttaactgc cctattataa
     4981 catacttagc ctaaacttgc ttgacttttt gctatttttt tatcgaaaaa tcgttttcat
     5041 aacaaattca cttaactctt cttctaattg aggcgttttc ataaccatgt ggtgctcagc
     5101 ctgaattgtc tggttactat cttgataatg aagtactact ggaatgcttc ctctatattt
     5161 atccaaaata cgagcaacag cataatcgtg ttctcgattt tccaaaagaa tccaaaattt
     5221 ctccaaactg acaggctcta atttttctag aactaattgc aactgtccat cgcgctcttg
     5281 cactctacct gtgatgtacc ccattccccc ttcttccaaa atagaagcgt attggcgata
     5341 agattctggg aaaagagtca cgtcaagcgt cctcttggta tctgtcactc gaagaaatgc
     5401 catctgctcc ccagtctttt tggtacgaat caatcgaata gcttgaatct gcacaagaac
     5461 ggtcgctcga ttgccaaccg ctaaatcagc caaatctgta aaactacgct tagactctcg
     5521 acaaatattg actaaaggat gcggactaat accaacacct agcaattctt gctctagcat
     5581 aaatttttca ctgtcgctgt aatcttctac atctgtccaa ctataactct cctctgcaaa
     5641 aaatgtacca atcgcctctg caaagacaaa gagattgtct agattttcaa taatcttttt
     5701 acgattggcg acaaaatcat caaataaacc aatctgaata agagggatta gtaaatcttt
     5761 tttcttgaaa ttatctggca agcgcaaaat aaaatcttct acagacttat agggacgatg
     5821 atccaatatc cataaagcta aatctttagg taaacctttt agtgttttta atcctaggta
     5881 gaccttactt ttatcaaact tatcgtgata aggaatattg ttaatcgtga gtcgagctac
     5941 ctcaaagtca aactgcatcg catcttcaat ataagcaccg ctagaatgat tgagcatgac
     6001 atcaaaaaag acatccgtat agtgactctt gaaataggcc atttgaaagg ctaaggctga
     6061 ataagcatag gcgtggctgc ggttaaaacc ataaccagcg aacttagcca ccatgccaaa
     6121 tatttccctt gcattctctt cagcatgtcc gtttttcagt gctccctgta aaaattccgc
     6181 ttccattacc tgcatttcct gggcattctt tttagacatc gccctgcgga gtaaatccgc
     6241 ctttccaagc gtaaatcctg catagacctg tgcaatctgc atgacctgtt cctgataaag
     6301 catgatacca taagtcggtt gtaaaatagg agcaatggtt gggtccaaca aatcaactgt
     6361 ctcttgtcca tgtttgcgtt ttacaaagtt atccgaataa tcactagcac ccggtctatt
     6421 caaactcgtt gttgcaacta tatcctcaaa tcgaccaggt ttgacccttc gaagcaggct
     6481 aatcgcacca ggctgttcaa attgaaaaat tcccttagtc cgaccagcag caaaaagttc
     6541 caaggttttc ttatcttcta aatctatctt ggcaatatca atttcctgct gataacgttc
     6601 acgtactcct tctgccattt tttgcacaaa ggtcagattt cgtaagccaa ggaaatccat
     6661 cttcaacaga ccattcgcct caacagcatg tgcatcatat tgggttacca gcatgtcatc
     6721 ccctatttta agagggatgg tatctgtcaa atcctcatca ctcatcacta tccctgctgc
     6781 atggatagaa gtctgtcggg gttgcccttc aatttgttga gcaatcgcaa aagctttttg
     6841 gtactctatc ttactattga taatctgacg aaaagcggca tttcgctgat aggcagaagt
     6901 aagactatcc ctaaatgaaa ttttcttggt aatattactt aactcatatt caggtgttcc
     6961 gaaacgctta aatacatcac gaatggcctg cttagctcca aatgtcgagt aggttacaat
     7021 ttgcgcagca tgggttgaac cataacgatc tcgtacataa cgaataaaat ctgctcgatg
     7081 aatatctgga atatcaatat caatatcggg cattgaataa cgttcagcat tgagaaagcg
     7141 ttcaaagaga agattatgct taacaggatc aatacctgtg atatttaagg cgtaggcaac
     7201 taggcttcca acagctgaac cgcgccccat tcccatgtag tacccttggc tacgcccaaa
     7261 acgaagtagg tcccatacaa tcaagaaata atcgtcaaat cccatttgat gaataatgga
     7321 taattccttt tccaatcgtt ccttatacag tgaattagtc aagcccttct cttctaatcc
     7381 ttgataggag cgctcgtata attcttccac tgcaggtctg tcacgattaa aacgcggcaa
     7441 ttttaaatct gtatttaatt gataagaaat ccctgcaacc aagtcacgta aattatcaac
     7501 tgcttgcgga aaccgattcg aaaatagatt ggttaaacta tcagccgata aaagcttttg
     7561 tcgactatct atatctacaa cttgattaag tggaatatta tctcgaatag catgtagaac
     7621 ctgtagactt tccaattgtt cctcttcaaa aaaacgaacc gtatgaattg ggagtattgc
     7681 ttgcttaaag tcttgaactg gtgtatcaac cgtcactccg atataatagt taacacctaa
     7741 ctcaaggtgc tcaatgccag aaaaataagg aacaaccact gctatgtcga ctaaatattg
     7801 acgaatagtt tcaaaatcat cttgccctat catcttagca gtcgaaattt tcaatagatt
     7861 gcgataacct ctggttgtca gagcaatcaa ttgagtagtt acgtcctgct ccccttcctg
     7921 taatttccag tccagttcac aaccaataat tggttgaagc cccattttgt tacaggattc
     7981 cataaaacta taggctgcat aaagactgtt tttatccatt atccccaatg cgtgataacc
     8041 gagctcttta gccctgccta cataattttc tatcgtcacc atgctatcca taaaggtata
     8101 aactgtcttt gtatctaatt gtgctagcat ggcattctcc ttttgttgct tcttcttaaa
     8161 ttatatcaaa taatcatcaa aaaatatttt aaaaaaattc aaaacatttc ccttgctaat
     8221 ttatatgatt tattgtatta ttaatataag acatacacac gggtgtcatc ctagaaagga
     8281 aacgattatg gcttggaaat ttgataataa catccccatc tatattcaaa tcagcaatac
     8341 tattaaattg cagattgtga ccaatcaatt aaaatctgga gataaacttc ccactgttcg
     8401 tgacttagcc gacattgctg gtgtcaatcc aaataccgtt cagcgcgctt tgtctgattt
     8461 agaaacagaa ggatttgttt actcagtacg cacaacaggc cgttttgtaa cggataatca
     8521 ggaattgatt acacaaactc gcctacattt agcccaaaag gaactggaga acttcgtgaa
     8581 caatatgtta gaattgggct ttagacacga tgaactcact agccagttag atgattattt
     8641 gaaaggagtt acttatgagt aacgcatata ctttggttga gctacagcaa gtttcaaaat
     8701 cttatggtgg agctgtcgcc ctaaataatg tcaatttgaa gttgactgct ggaaaaatca
     8761 taggactttt gggaccaaat ggttcgggta aaacaaccat gattaaattg atcaacggtc
     8821 tgttgcagcc agaatatggt caagtattga tcaatggtcg cacaccatcg ccagaaacca
     8881 aggcgattgt atcttatctc ccagatacca cttatcttga tgaacatatg aaagtgctcg
     8941 aagcaattac attttttgaa gatttttacg ctgattttga taaggagcgt gctctccatc
     9001 ttttacaaga tttgggcatt gatttaaaca gtcgtatgaa acatctctct aaggggaata
     9061 aggaaaaagt ccagctcatt ctggtgatga gccgtcaagc acagctgtat gttttagatg
     9121 agccaattgg tggtgttgac cctgccgccc gagactacat tttaaaaacc atcgtcaaca
     9181 actactctcc gactgcttct gttattatct ctactcactt aatttcagat gttgagcaaa
     9241 ttttggacga ggttattttc ctacactacg gaagtgttat tcgccatggg aatgtagatg
     9301 acttgcgaat tgaaagcgga gaatctattg acgaactctt ccgccaagat tttaaggctt
     9361 agttagaagg agattgctat gtttaaaaaa ttattgaaat acgaatttca atccgtaggt
     9421 aaatggtatt tgggaattta tattgcagct gtggtcctat cagcagtact tggtttttgg
     9481 ctacaagcgt tggccctccg tacacaggct ggaaatactg aacctggcgg tggagagatg
     9541 gtcttattgg gaacatcctt tatgactttt ggtattttga ttgccgcact cttcttgtca
     9601 acatttgttc tagtaatcaa ccgttttaga aaaaatgttt atggccgaca aggttatctc
     9661 actatgaccc tgccggtatc tagctatcag atcatcttaa gcaaactact tgcctctatt
     9721 gtttggtata tcttggcagg tattacttgc ttactttcaa ttggaatcat ccttgcagtc
     9781 attctagcag caagcgaaga agctattatg ccagaaatac aaacttttgt tcaagctatc
     9841 gactggacag ttctattcag tcagattttc tattatttaa ttgaaattac cactggtatc
     9901 cttttgatct acttctctat atctgtcggt caattattca aggatcatcg cttcctcttt
     9961 gcaatcttga cttatatcgg cattctcatc gttactggag tatttagcac tttctttgtc
    10021 ctaagtaacc taggaacagc ttattctaca ccattttctc tctatccaag tccagtctta
    10081 gcattagtca atattatcct agcttttgct tactactttg gaacccactt cattatgaca
    10141 aagaaattaa atctacaata aaaagctctt tactggtcta atcgaccggt atagagcttt
    10201 tttcttaata ctgttggtct aggagttcca acaaaacttt cttttctcct tcgctggcaa
    10261 aacttgcctg aatagcattt tgattgaagc gataaaaatc agccttgcta gtcccaaaat
    10321 attctacaaa taactgatat tctctattaa gtgtagtatt agaaacggta cgattatctg
    10381 tgttgattgt gatgtttgct cctgcctcaa ctagctcacg atatggaaaa tattcaatcg
    10441 aatcagctgc accagtttga agattcgatg ttagacacat ttcaagtgtt gctccgctat
    10501 tgacaaaagc ttggatagct tcaggattgc gagtaagtgc tgtgccatga ccaatccttc
    10561 taatcccaag ttcaagagct tgaatcacat tgattacaca tccacattct ccggcatgga
    10621 aagtcatggg ataacctaaa gactgagtaa attgaataat atcccgaagt tcttccgtag
    10681 gatagtctgc ttcatttcct gcgaaatcaa atcccacaag tccttttggt gccaaatcag
    10741 caatagccga aaagagttcc ttcgtttgat tcgtattcgt ttgctttaaa ccacaaacca
    10801 gaagtttagc aagcactcca aaatcttcct gtcccctgtt tagtccaact aaaactgctg
    10861 agactgcttc taaaatagtc aagtcttgat ccgtcgacag ttcaggtgca aaacgaacct
    10921 caatgtaaat gaccttatca cttgcagctt gtcgtacaag atcataggct gccaattcta
    10981 aagctgttgc agtttgtaag agtgggcgaa caaaatcaaa taactttaaa taagccatta
    11041 aactatcaac ttttccatga acactaacta attttcttaa tgcttcatct tctgcaggaa
    11101 tagtaatctt agccatctgt gccaattgtc gaatgactcc gagggacaat gagccatcca
    11161 aatgacaatg caactctgtt ttgaccaatt catttacatt caacactatt aacacaacct
    11221 ccgtctggta tattgttaac tatcatatca tatttttgaa gaaaaacaag aattttacca
    11281 taaaacctga actttcactt gtttttcagt tgtatcattc acagacaaaa aaagaacgac
    11341 tatcagtcgt tcttcaataa catacggaag acatgggatt cgaacccacg cacgctgtta
    11401 cacgcctacc gcgtttccaa cacggcctct tgagcctctt gagtaatctt ccatatatgg
    11461 agctggtggg agtcgaaccc acgtccaaac acctgctaac ttattcgtct acaaccatag
    11521 gttatgtctt gctttaactt gaactcgata cataactcaa acctagacca agcgagtcag
    11581 taaatctctt ttggaactgc ctgactcaat cccaacgtag cttgctattt taagaccaat
    11641 cagcaaacac aagcaattcg cgactggtca cgcaggcagg ttattaagct gctaatgcgt
    11701 aagtgtttgt attttttgca gttatattta actggcattt ttacatctgc cgatgagttg
    11761 cagaacaagc ctcataatgc ctgtcgaatc cgtaacaacc ccgtaggatt tgatacagga
    11821 attattatag cagaattttt tataaatagc aaaattattt tacat
//
LOCUS       DASFSW010000062        11599 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.062, whole genome shotgun sequence.
ACCESSION   DASFSW010000062 DASFSW010000000
VERSION     DASFSW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.062"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1257)
                     /locus_tag="U1099_001203"
     CDS             complement(<1..1257)
                     /locus_tag="U1099_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEM4867523.1"
                     /translation="MYSRRKALVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLL
                     GEGLNQVFNPLWLFSFTQKSLLAFILGVLTMTLVYLYVSTGQKVYREGEEYGSARFGT
                     SKEKWNFYSKNPLNDTILARDVRLTLLEKKKPQFDRNKNLIVIGGSGAGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRG
                     RYPAFSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTL
                     DSVIAVTTAKFALFNIQSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALF
                     FSTVFSTLTRQADVDFKGQLPIHVRSY"
     gene            complement(1257..1745)
                     /locus_tag="U1099_001204"
     CDS             complement(1257..1745)
                     /locus_tag="U1099_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867524.1"
                     /translation="MSSEQQERQAMQYVERSSLLTVRTLLKLLEWSARQALAQDSAYK
                     IGVQKLEELLQSPYVIEAIDVSKDILDKPIDVEKFKELMESEKLPIAISWQKDYLYFY
                     AKDKTLLDHHLDELLKKLMSNPEKLEGLTFDKTLDQEIELAKEKIRVTEPSAVKTKEV
                     TL"
     gene            complement(1823..2407)
                     /locus_tag="U1099_001205"
     CDS             complement(1823..2407)
                     /locus_tag="U1099_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4867525.1"
                     /translation="MIRIVVFYLAIQLNGLLVSLYLKEYLTIEGIVLLQLVLLSVTCL
                     EIARHKTVQAKNITLRNRLRWLLLGFVAMVAFAVFISFLFPGQTRNQAVLVQVGKQVP
                     PIIFLLFLVNASILEEIVYRQLLWEKLTFPFVQIGVTSFLFVLSHGPNQLGSWLMYSC
                     LGLTLAAVRLKADCMTAIALHLLWNSLAYVVTFL"
     gene            complement(2410..2643)
                     /locus_tag="U1099_001206"
     CDS             complement(2410..2643)
                     /locus_tag="U1099_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM4867526.1"
                     /translation="MNERFWDNLEIILAEKELTWAELARKVFNGQYVYPSEFNRLYQK
                     LRHYKSNRLMPQTRWVERIVLVLEIDYEDLFKR"
     gene            complement(2652..3035)
                     /locus_tag="U1099_001207"
     CDS             complement(2652..3035)
                     /locus_tag="U1099_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867527.1"
                     /translation="MLEVYLGNNANTNQDLLTILTTYGVTYRCIKACDVDREILLSLF
                     AKTMDCFELLSPRFLCFKRQYSISLNEMIQLILQKPDQNLRLPLIVCQNHVYPAIGLD
                     EVRTFLPRQVKEELFQASLMKQVTG"
     gene            complement(3046..3474)
                     /locus_tag="U1099_001208"
     CDS             complement(3046..3474)
                     /locus_tag="U1099_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867528.1"
                     /translation="MNLLHKKSILDCTELEERIHQAETNQLLQKILSLPNFDCDFEVT
                     FEDDYHKEMNDPLFYESNLHQISDFMETRDIKNGVDTLLTKDNHLAFRAFGENYSARG
                     KDGILTTLVIVKCFGEGRMPIDMSRYFSTPEPTVENSLIL"
     gene            complement(3458..4813)
                     /gene="dcm"
                     /locus_tag="U1099_001209"
     CDS             complement(3458..4813)
                     /gene="dcm"
                     /locus_tag="U1099_001209"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEM4867529.1"
                     /translation="MKFLDLFAGIGGFRLGLESQGHKCLGFCEIDKFARTSYKAMFNT
                     EGEIEYHDIKEVTDHDFRQFRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDEGRTFATILSTMDELGYDVEWQVLNSKDFQVPQNRE
                     RVFIIGHSRRYRSRFIFPLRRENSPAHLERLGNINPSKRGLNGEVYLTSGLAPTLTRG
                     KGEGAKIAIPVLTLDRLEKRQHGRRFKDNQDPMFTLTSQDRHGVVVAGNLPTSFDQTG
                     RVFDISGLSPTLTTMQGGDKVPKILLREELPFLKIKEATKTGYAKATLGDSVNLAYPD
                     STKRRGRVGKGISNTLTTSDNMGVVVAALEYRQDKWYEVTGIVLEGKVYRLRTRRLTP
                     RECFRLQGFPDWAYERAESVSSKSQLYKQAGNSVTVTVIEAIAREFRRTEEEEKHELT
                     T"
     gene            complement(4962..5780)
                     /locus_tag="U1099_001210"
     CDS             complement(4962..5780)
                     /locus_tag="U1099_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM4867530.1"
                     /translation="MKRITANQYQTSERYYKLPKILFESERYKDIKLEVKVAYAVLKD
                     RLELSLSKGWIDEDGAIYLIYSNSNLMALLGCSKSKLLSIKKTLRDYGLIDEVQQSSS
                     EKGRMANKIYLGELEHETTPVLHTDGASVKKTLGESQRKTGPVLNSAPSETEGSETKY
                     SETEGSDFLIEDEEERQLVDEKQEENFTSKVDGVTKYDRDYIWGLVHDQLRQTGLSQS
                     ASDYAMIYFSDRYQYALEHMRFARSAEVIAEYVFNGVLSEWTKQLRRQEVKGGE"
     gene            complement(5777..5950)
                     /locus_tag="U1099_001211"
     CDS             complement(5777..5950)
                     /locus_tag="U1099_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867531.1"
                     /translation="MKKKEQSSRQIVMCHLVAILGVDIVKATQLIDEMEQPGLIRFDE
                     FGNVGILVLEGQS"
     gene            complement(6161..7522)
                     /gene="rlmD"
                     /locus_tag="U1099_001212"
     CDS             complement(6161..7522)
                     /gene="rlmD"
                     /locus_tag="U1099_001212"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEM4867532.1"
                     /translation="MLKKNDIVEVEVVDLTHEGQGVAKIDGFVFFVDNALPGEKISMR
                     ILKLKKNIGFGKVEQWQSFSQDRNQDLDFTYLRTGIADLGHLTYPAQLAFKKKQVENS
                     LRKIAGISEIAVADTLGMDNPLAYRNKAAVPVRRVNGQLETGFFRKNSHDLVPIEDFY
                     IQHKEIDALVLATRDLLRKLDLKPYDEKEQTGLVRNIVVRRGHYSGQLMLVLVTTRPK
                     IFRVETLIERLTSQFPNLVSIVQNINDQNTNAIFGQEFRLLHGSETIADTMLGNEFEI
                     SAPSFYQVNTEMAEKLYQTAIDFAELSADDVVIDAYSGIGTIGLSFAKQVKHVYGVEV
                     VERAVLDSQKNAARNGIDNVTYVCDSAESAMQKWVADGVKPTVIFVDPPRKGLTESFI
                     KASTSVKPEKIVYISCNVATMARDIKLYEELGYKLTKVQPADLFPNTHHVECVVLLQR
                     SNG"
     gene            complement(7589..8914)
                     /locus_tag="U1099_001213"
     CDS             complement(7589..8914)
                     /locus_tag="U1099_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM4867533.1"
                     /translation="MNKNNNILTHHEQLRLDYLQKNIHYLNDKESRELDYLLYKKELR
                     ERHGSPRQSLRSFTPTAYEDDYFEEDYFEEDDEQEELLPRYPERKSRKTRQAKPQRFQ
                     SPRIGPKAKVPKPKKARKKGGFKRFIGFVLLAIALVIVAMGYSFFKGMNSVSEKPVAE
                     VFNGVDTSNGTNILILGTDGRVGESSGETRTDTIMVLNVNNTDNKVKLVSFMRDTLVD
                     IEGYEYKLNTAYTLGEQDNQQGAEEVRKALKNSFDIDIKYYAMVDFATFATTIDTLFP
                     EGVTIDAQFSTINGEVVSSVEVPNDLQLEENAPAYQTIQVGVQQMDGKTLLNYARYRS
                     DDEGDFGRTRRQQEVLSAVMTQAKNPMKLFSGSEAIGKVVAMTPTTVPQSFMWLQGPG
                     LLLDAANGIERVTIPENGDWIDDYDMYGGMALRVDFEKYQQRLAELGLR"
     gene            complement(8926..9753)
                     /gene="pheA"
                     /locus_tag="U1099_001214"
     CDS             complement(8926..9753)
                     /gene="pheA"
                     /locus_tag="U1099_001214"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEM4867534.1"
                     /translation="MQVAYLGPRGSFTHQVAQQVFPTADLQAFETITEVIKAYETGEV
                     TYSVIPVENSIEGSVHETIDYLFHQAEIHAVAEIVQPIAQQLLATSADKAVEVIYSHP
                     QAIAQGKKYIQEHFPQARIEITVSTAYAARFVAEHPEQPFAAIAPQAAAVEYGLEVIA
                     QDIQEISENFTRFWILGDKAPSIALEQVAKKVSLALTLPDNLPGALYKAMSVFSWRGI
                     SLTKIESRPLKTALGEYFFILDLDNQSEELLTYSQKELTSLDITYKVLGNYLVYMTS"
     gene            complement(9744..10235)
                     /locus_tag="U1099_001215"
     CDS             complement(9744..10235)
                     /locus_tag="U1099_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEM4867535.1"
                     /translation="MPIVLLGFMGVGKTTTAHLLNLPVYDMDHIIEERIGMPIADYFS
                     LEGEASFRQLETEVLKELLDLPSNCIVSTGGGVIKSEVNRELLLANREDNVLLTASFE
                     VSYQRIRKDRQSQRPLFLQCSKEEFEALYRERMALYQGLADTVIDTDKLIPEQVARKI
                     LCK"
     gene            complement(10244..11524)
                     /gene="aroA"
                     /locus_tag="U1099_001216"
     CDS             complement(10244..11524)
                     /gene="aroA"
                     /locus_tag="U1099_001216"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEM4867536.1"
                     /translation="MKLRGSVEKLKGTIRVPGDKSISHRSIIFGSLAKGVTRFHDILR
                     GEDVLSTMQVFRDLGVKIEDNGDIVEVHGVGFDGLQAPKNDLDMGNSGTSIRLISGVL
                     AGQDFEATMFGDDSLSKRPMDRVTIPLSQMGVEISGQTERDLPPLTIKGNKNLKPIRY
                     QLPVASAQVKSALIFAALQAEGESVIVEKELTRNHTEDMIVQFGGQLEVNGKEIRIQG
                     GQEFIAQEITVPGDISSAAFWLVAGLIIPGSKIVLENVGINETRTGILDVIKAMGGKM
                     TFSNIDELAKSATITVETSELKATEIAGELIPRLIDELPIITLLATQAHGTTIIRDAE
                     ELKVKETDRIQVVADALNSMGATIEPTEDGMIIHGPTALHGAEINTFGDHRIGMMTAI
                     AALLAKDGEVVLERAEAINTSYPAFFEHLNSLMD"
BASE COUNT         3324 a         2671 c         2053 g         3551 t
ORIGIN      
        1 atagcttcta acatgaatag gcagttgccc tttaaagtca acatcagcct gtctggtcaa
       61 agtggagaaa accgtagaga aaaataaagc agataggaaa cgaaaggtag tgtcattatc
      121 tggaataaca agatagacca tggtcttttg agtgccccac gttttcaaat ccatagtgtc
      181 tctttgcgtc aaatcaatca ccgattggat attaaagagg gcaaatttag ctgttgtgac
      241 cgcaatcacc gaatccaagg tcttgtcctt gtagttctga aaatccgccc agtttctcat
      301 ggtaaagttc tcatgaccgt attttttagc gtaatcttca aacaggactt caagaacact
      361 tttgtcctga ttatctccct ttgataagag tttgatgagt ttcccaatct cagaaaaggc
      421 tggataacgg ccacgcttac gtcttgcttc ctgctcttgc ttggaacttc ctggaggatt
      481 gtaaaaatct accaaataag aggcaatagc tctcaccaat gtcatggaag cctcgtccca
      541 aaatggatca ctgcgagaac catttccttt ggtattatta aaatagaccg ttaacatgcg
      601 gttcaaatca ttctctgttt ctacataacg aaagggatta aaaccgtccg aattggtcat
      661 attaaccaag tctaaaacct tgacctgata accgttctct aaaaagagtt tacctgtctt
      721 ctcagccaaa tggtctttcg gatctacgac aatattggaa caattaagtt ggataaggtt
      781 gggtttcaca aagcgaaagg tcttacctgc cccagaaccc ccaatgacaa tcaaattttt
      841 atttcggtca aactggggtt tcttcttttc caacaaggtt agacgaacat cacgagccaa
      901 aatcgtgtca tttaaaggat tcttactgta aaagttccac ttttccttac tggttccaaa
      961 tcgtgcagaa ccgtattctt ccccttctcg atacaccttt tgtcctgtcg atacataaag
     1021 atagactaat gtcatcgtta gaaccccaag gataaaggca agaagagatt tttgagtaaa
     1081 ggaaaatagc cataaaggat tgaagacttg atttagcccc tctcctaaga ggtaggcaaa
     1141 acgttccata ggtggggcat tggttaaact atcatagagc agggttagac gatggcagaa
     1201 ataaccaaag gcgagaccca agagtccaaa gactaaagct tttcgtcttg aatacattac
     1261 aaggtcacct ccttggtttt gactgccgaa ggttctgtca ctctaatttt ctctttggcc
     1321 agttctatct cttggtccaa ggttttatca aaggttaaac cttctagctt ctctggatta
     1381 gacatcagtt tcttcaacaa ttcatccaag tgatgatcga gcaaggtctt atccttggca
     1441 taaaagtaga gatagtcttt ttgccaacta attgcgattg gcagtttttc tgactccatc
     1501 aactccttaa atttctccac atcgattggc ttatctagaa tgtctttact aacatcaatc
     1561 gcttcaatga cgtaagggct ttgaaggagt tcttccagtt tttgaacccc aatcttataa
     1621 gctgaatctt gagccaaggc ttgcctagcc gaccactcta agagctttaa gagggttcta
     1681 actgtcaata aactacttct ttccacgtat tgcatcgctt gacgttcttg ttgttcagat
     1741 gacatggtga cctcctcttt ttttcgattc cctatcaaac ataaggtgct tccataatac
     1801 ggaagcactc ttgattttgg tatcacaaga aagttacgac ataagccaaa ctattccaaa
     1861 gtaaatgtaa ggcgattgcc gtcatacaat cagctttcaa tcgaacagca gccaaggtca
     1921 agccaagaca gctatacatg agccaactcc ctaactgatt ggggccatgg gatagaacaa
     1981 agagaaaact ggtcacgcct atttgtacaa aagggaatgt caatttttcc cacagcaatt
     2041 gcctataaac aatctcttca aggatacttg cattgaccag aaacaataaa aagataatag
     2101 ggggaacctg ttttcctact tgtaccaata ccgcttgatt cctagtctga cctggaaata
     2161 ggaagctgat gaagactgca aaagcaacca tagccacaaa gccaagaagc aaccatctta
     2221 ggcgatttct caaggttata ttttttgctt gaacagtttt atgacgggca atctctaagc
     2281 aagtcacact taataggacc aattgtagca agactatacc ctctattgtc agatactctt
     2341 tcaggtataa actcaccaga agaccgttaa gctgtatagc tagataaaac accactatcc
     2401 ttatcattgt cacctcttaa ataaatcttc ataatcaatt tctaagacta gaacaatacg
     2461 ctcaacccat cttgtttgtg gcatcagacg attggatttg taatgtcgca gtttttgata
     2521 gagacgatta aactcacttg gataaacata ttgaccgttg aatactttac gtgctagttc
     2581 tgcccaagtg agttcttttt cggccagaat gatctctaaa ttgtcccaaa atcgttcatt
     2641 catcttcaca cctaacctgt cacctgtttc atcagactgg cctgaaacag ctcttctttt
     2701 acttgtctgg gaagaaaggt acgcacttcg tctagcccaa tagctggata gacatgattc
     2761 tgacagacaa tgaggggcaa ccgaagattc tggtctggct tttggagaat aagctgaatc
     2821 atctcattca aacttatcga atattgacgc ttaaagcaaa gaaatcgtgg cgacaataac
     2881 tcaaaacaat ccattgtttt ggcaaagagt gacagtaaga tttcacggtc tacatcacac
     2941 gcttttatac aacgataagt cacaccatag gtcgtcaaaa tggttagtaa atcttgattt
     3001 gtattggcat tatttcctag atatacctca agcataggca cctccttata ggattaggct
     3061 attttcaact gttggttcag gagttgagaa atagcgactc atatcaatgg gcatccgtcc
     3121 ttcaccaaag cacttgacta tcactaaagt cgttaaaatg ccatcctttc ctctagcaga
     3181 ataattttca ccaaaggcac gaaaggccag gtgattgtct ttcgtcagta gtgtatctac
     3241 accattttta atatccctag tttccataaa atccgaaatc tgatgaagat tggattcgta
     3301 gaatagggga tcattcatct ctttgtggta atcatcttca aaagtcacct caaagtcaca
     3361 atcaaaattg gggagcgaca gtatcttttg taatagctga ttggtttcag cttggtgaat
     3421 acgttcttct aattcagtac aatctaggat acttttctta tgtagtaagt tcatgttttt
     3481 cttcctcttc cgttcttcta aattctctgg caatggcttc aataactgtg acagtcacgc
     3541 tattgccggc ctgtttgtat agctggctct tactggaaac actttctgct ctttcataag
     3601 cccaatcagg aaagccttga agtctgaaac actctcttgg tgtcagtcgt cttgttctca
     3661 ggcgataaac tttcccctct aagacaatgc ctgtgacttc ataccactta tcctgtcgat
     3721 attccagagc agcaaccacc actcccatat tgtctgaagt cgtcagagta ttggatattc
     3781 cctttcccac acgtccccta cgtttggttg agtctggata agccagattg acagagtctc
     3841 caagagttgc ctttgcgtac cctgtttttg tggcttcctt gattttcaga aatggcagct
     3901 cctccctcag caaaatcttt ggcaccttat ctccaccttg catggtggtc aaagtcggtg
     3961 acaagccaga tatatcgaat actcttccag tctggtcaaa gctagtcggc agatttcctg
     4021 cgacaacaac cccgtgtctg tcttgacttg tcaaagtaaa catagggtct tgattgtcct
     4081 taaatcgacg accatgttgc cgtttttcta gtctatctag tgttaagact ggaatggcga
     4141 ttttagctcc ctctccttta cctcttgtta gtgtaggagc aagtccactc gtcagataga
     4201 cttcaccatt caaaccacgt ttagagggat tgatatttcc tagcctttca agatgagctg
     4261 ggctgttttc tcttctgaga ggaaatatga atctggaacg gtatcttcta gaatgtccga
     4321 taataaagac ccgctctctg ttttgcggga cttggaagtc cttactgtta agcacctgcc
     4381 attcgacatc ataccccaat tcatccatcg tggagaggat tgtggcgaac gtccgtccct
     4441 cgtcgtgatt gagtaggcct ttgacgtttt ccaaaaatag aaaacgtggt tggatttgtt
     4501 tggccgctcg agcaatctca aaaaagagag tccctcgagt atcttcaaat cccagtcgtc
     4561 ttcctgcgag tgaaaaagct tggcaaggaa atcccccgca gatgatgtcc acttgccctc
     4621 taaattgtct aaagtcatgg tctgtgacct ctttaatgtc atggtattct atttcccctt
     4681 ctgtgttaaa catggcttta taagatgttc tggcgaattt atcaatttca caaaagccca
     4741 ggcatttatg accctgtgat tccaacccta gcctaaaacc acctatccca gcaaataaat
     4801 ctaaaaattt cattttttct ctctttccaa ctaaaaaaaa gccttacaca tgtaaaactc
     4861 ttgcttatat atgatcatcc atttctggtg cacggatgat ttcaacaatc aaaatgatga
     4921 tagaaatcag atagattcca cctagtatcc accaaatcat cttattcacc tccttttact
     4981 tcttgtcgtc tcagttgctt ggtccactct gacagcacac cattaaatac gtattcagct
     5041 attacttccg ctgaccgagc aaatcgcata tgttccaaag catactgata acggtcacta
     5101 aaataaatca tggcatagtc actagctgac tgagatagac ctgtctgtct taactggtca
     5161 tgaaccaaac cccaaatata gtctcgatcg tacttggtca cgccatcaac ttttgaagta
     5221 aagttctctt cttgtttctc atctaccagc tgcctctcct cctcgtcctc aataaggaaa
     5281 tcactccctt cagtttcact atatttagtc tcacttcctt cagtctcact aggggctgaa
     5341 tttaagaccg gccccgtctt tctttgagac tcccctagtg tttttttaac actagccccg
     5401 tcagtatgta agactggggt agtttcatgt tctaattccc ccaagtaaat tttatttgcc
     5461 attcgacctt tttcactaga ggactgttgg acttcatcaa ttaagccata gtcacgtaag
     5521 gttttcttga tagagagtaa ttttgacttt gaacagccta aaagtgccat cagatttgaa
     5581 ttggaataaa tcaagtaaat agccccatcc tcatcaatcc agcccttact caaagacagc
     5641 tccaacctat cttttaaaac cgcgtaggct accttaacct ccagctttat atccttataa
     5701 cgttcactct caaacaaaat tttagggagt ttataataac gctctgatgt ctgatactga
     5761 ttagcggtaa ttcgtttcat gattgtccct ccaagactaa tattccaaca tttccaaatt
     5821 catcaaatcg gattaaacct ggttgttcca tttcatcaat tagctgtgtt gctttcacaa
     5881 tatcaactcc taaaatagcc acaagatgac acatcacgat ttggcgtgat gattgttcct
     5941 tctttttcat gaattcctcc tgaaaataaa aaaacgagaa cacttagtaa aatgttctcg
     6001 ctgaactatt attttttatg acgtatctat tttttgtcta cgcagtcgac atccaaacag
     6061 acattcacat caatgtactt tttttgaacc ttttttatgt caaaatcact cttttcaata
     6121 aagacaaaat gcttgaaaaa gtgcttaaaa taaaggattt ttacccgttg cttcgttgta
     6181 gcaacactac gcactccacg tgatgcgtat ttggaaataa atcagccggc tgtaccttcg
     6241 tcaatttgta ccccaattcc tcataaagtt taatatcacg cgccatggtt gccacattac
     6301 aagagatata gacgattttc tctggtttta cagaagtact tgctttgata aaactttctg
     6361 tcaaaccttt tcgtggtggg tcgacaaaaa tcacagttgg tttgactcca tctgccaccc
     6421 atttttgcat agcggattca gcgctatcac agacataagt aacattatca atcccatttc
     6481 gagcagcatt cttctgacta tcaaggacag ccctttcaac gacttctaca ccgtaaacat
     6541 gcttaacttg ttttgcaaag gagagaccaa ttgtcccaat acctgaatag gcatcaatca
     6601 cgacatcatc tgccgacaat tctgcaaaat caatggcggt ctgatagagt ttttcagcca
     6661 tttctgtgtt gacttgatag aaagacggtg cagaaatttc aaattcattg cccaacatgg
     6721 tatctgcaat agtttcactg ccatgcaaaa gacggaattc ttgaccgaat atagcattgg
     6781 tattctggtc attaatattc tgaacgatag aaacaagatt tggaaattgg cttgtcaaac
     6841 gctcaataag ggtttcaaca cggaaaatct tcggacgagt cgtaactaaa actagcatca
     6901 actgacctga ataatgacca cgacgaacaa caatatttct cacaagtcct gtctgttctt
     6961 tctcatcgta aggcttcaag tctaattttc tcaagaggtc acgtgtcgct agaaccaagg
     7021 catcaatttc cttgtgttga atgtaaaaat cctcaatcgg aactaaatca tgtgaatttt
     7081 ttcgaaagaa tcctgtttct aactgcccat tcacacgacg aactggaaca gctgccttat
     7141 tacgataagc aagaggattg tccattccca acgtatctgc tactgctatt tctgaaatgc
     7201 ctgcaatctt acgtagacta ttctcaactt gttttttctt aaaagctagt tgggctggat
     7261 aagtgagatg ccccaagtca gcaatacctg ttcttagata ggtaaaatcc aagtcctgat
     7321 tacggtcttg tgagaagctc tgccattgct ccaccttacc aaaaccaata tttttcttaa
     7381 gttttaagat gcgcatggaa attttctcgc ctggaagagc attgtctaca aagaagacaa
     7441 aaccatcaat ttttgccaca ccctgcccct catgtgtcaa atctacaact tctacttcga
     7501 caatatcgtt tttctttaac ataattctac tttctatttt tggaccacta aaaaagtgac
     7561 cgaagtcact tctagtatac catatttctt aacgcaggcc taactcagcc aagcgctgct
     7621 ggtatttttc aaaatcaaca cgaagagcca taccaccata catatcatag tcgtcaatcc
     7681 agtctccatt ttctgggatt gtaacacgtt cgataccatt tgccgcatcc aatagaaggc
     7741 ctggtccctg tagccacata aagctttgcg gtactgttgt cggagtcatg gccacaacct
     7801 tacctatagc ttcggagcca gagaatagtt tcattggatt tttagcctgt gtcataacag
     7861 cagatagcac ttcctgttga cgtctcgtac gaccaaaatc accttcatca tccgaacgat
     7921 agcgcgcgta gttaagcaag gtcttaccat ccatctgctg tacaccgacc tgaatcgttt
     7981 gataagcagg agcattttct tccagctgta aatcattcgg cacttctact gagctaacga
     8041 cttcaccatt aatagttgaa aactgtgcat caatcgttac gccctcaggg aaaagtgtat
     8101 caatcgttgt cgcaaaggtt gcaaaatcca ccatcgcata gtacttgata tcaatgtcaa
     8161 aactattctt caacgccttc cgaacttctt cagccccttg ctgattatcc tgttctccca
     8221 aagtataggc agtattgagt ttatattcat atccctcaat atccaccaag gtatcccgca
     8281 taaagctaac aagtttgact ttgttatccg tgttattcac atttagaacc atgatggtat
     8341 ccgttcgagt ttcacctgag ctttcaccaa cacgaccgtc tgttcccaaa attaaaatat
     8401 ttgtcccatt cgatgtatct acgccgttga agacctcagc tactggcttc tcactcacgc
     8461 tattcatacc tttgaaaaaa ctatagccca tcgctacaat cactagcgct atagcaagca
     8521 atacgaaacc aataaagcgc ttgaaaccac cctttttacg agctttttta ggtttaggta
     8581 ctttcgcttt gggaccgata cgaggagact gaaaccgttg aggtttggct tgtcttgttt
     8641 ttctggattt acgttcagga tagcgaggca acagttcctc ttgctcatca tcctcttcaa
     8701 aataatcttc ctcgaagtag tcatcttcat aagccgttgg tgtgaagcta cgtagagatt
     8761 gccttggact tccatgccgt tctctcagct cttttttata taatagataa tccaactcgc
     8821 gactttcttt gtcatttaag tagtggatat ttttttgcaa ataatctaac ctcaattgtt
     8881 catgatgggt taggatatta ttgtttttat tcatctgtct tcatcctagc tggtcatata
     8941 gaccaggtaa ttccctaata ctttataggt tatatctaaa ctagtcaatt ctttttgaga
     9001 ataggttaat aattcctcag actgattgtc aagatccagt atgaaaaaat actcacccaa
     9061 agccgttttc aggggtcggc tttctatttt tgttaagctg attcctctcc atgagaaaac
     9121 agacatggcc ttatacaagg ctcctggcag gttatctggc aaagtcagtg caagactgac
     9181 ttttttagct acttgttcca gggcaataga cggagcctta tcccccaaaa tccaaaagcg
     9241 tgtaaaattc tcgctgattt cctggatgtc ttgcgcaatt acttccagtc catattcaac
     9301 tgctgccgct tgcggagcaa ttgccgcaaa tggttgttca gggtgttctg ccacaaagcg
     9361 agcagcatag gctgtactaa ctgttatttc aatacgagcc tgaggaaaat gttcctgaat
     9421 atatttcttc ccctgagcaa ttgcctgcgg atgagaatag ataacctcta cagccttatc
     9481 ggcactcgtg gctaacagtt gttgggcaat cggttgaaca atctctgcaa ccgcatgaat
     9541 ctccgcctga tgaaagaggt agtcaatcgt ttcatgaaca ctaccttcaa tcgagttttc
     9601 tactggaata acggaatagg taacttcccc cgtttcgtag gccttgataa cctctgtaat
     9661 cgtctcaaaa gcctgcaaat ccgcagtcgg aaagacctgc tgggccactt gatgtgtaaa
     9721 agaaccacga ggtcctagat aggctacttg cataaaattt tccttgctac ttgttctgga
     9781 atcagtttat ctgtatcgat tacagtatcg gctaaacctt gataaagtgc cattcgttca
     9841 cgatagaggg cttcaaactc ttccttacta cactgtaaaa agagaggtct ttgcgactgt
     9901 ctatccttac gaattctctg ataggaaact tcaaaggagg cagtaagaag tacattgtcc
     9961 tccctatttg ccaacagcaa ttccctgttt acttcagatt taatcacacc accaccagtt
    10021 gatacgatac aattactagg taagtccagc aattctttca gtacctctgt ctccagttgt
    10081 ctaaacgaag cctctccttc aagactgaaa tagtcagcaa taggcatacc aatccgttct
    10141 tcaatgatat ggtccatatc gtaaacgggg agatttaaca aatgtgctgt tgttgttttt
    10201 ccaactccca taaatccaag taaaacgatt ggcatacgtt ctcctaatcc atcaaactat
    10261 ttaagtgttc aaagaaggca ggatagctag tattgatagc ttctgctctt tccaatacaa
    10321 cttctccatc tttggccaac aaggcggcaa tggcagtcat cataccgata cggtggtcac
    10381 caaatgtatt gatttcagca ccatgtaaag cagttggtcc gtgaataatc ataccatctt
    10441 ctgttggttc aatagttgct cccatactat ttagagcatc tgcaacaacc tgaatacgat
    10501 ccgtttcttt aactttcaac tcctcagcat cacgaataat cgttgttcca tgtgcttgag
    10561 tcgctagtaa ggtaataatc ggcaactcat caatcaaccg aggaatcaat tcgcctgcaa
    10621 tctccgtagc cttcaattcc gacgtttcaa ctgtaatggt agcagatttt gcaagttcat
    10681 ctatgttaga aaaagtcatt ttaccaccca tagctttaat gacatctaaa ataccagtcc
    10741 gagtttcatt gattcccaca ttttcaagga caatttttga acctggtatg attaagccag
    10801 caaccaacca aaaagcagca cttgaaatat ctcctggaac tgtaatctct tgggcaataa
    10861 actcctgacc accttggatg cggatttcct tgccattgac ttctaactgt ccaccaaact
    10921 ggacaatcat atcttctgta tggtttcgag tcaactcttt ttctacgatg accgactctc
    10981 cctcagcctg caaggcagca aaaatcagag ctgattttac ttgagcagag gctacaggga
    11041 gttggtagcg aattggtttt aaattcttat tccctttgat ggtcaacgga ggtaggtcac
    11101 gttcagtttg tccagaaatc tctactccca tttgactgag tggaatagtt actcggtcca
    11161 taggacgttt ggataaggaa tcatcaccaa acatagtcgc ctcaaagtct tgacctgcta
    11221 aaacgcctga aatcaaacga atcgaggttc cagaatttcc catatccaaa tcatttttgg
    11281 gggcttgaag accatcaaag ccaacaccgt gtacttcaac aatatcacca ttgtcttcaa
    11341 tttttacacc caaatcacga aagacctgca tggttgacaa gacatcttcc ccacgtaaaa
    11401 tatcgtggaa acgggtaaca ccttttgcaa gggaaccaaa aataatagaa cgatgactga
    11461 ttgatttatc acctgggaca cgaatcgttc cctttagttt ttctacgctt cctcttaatt
    11521 tcatatcctt acctcaaact agtatcactt tcattttatc atatttataa aatattttct
    11581 gatttttatg acaattact
//
LOCUS       DASFSW010000063        11528 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.063, whole genome shotgun sequence.
ACCESSION   DASFSW010000063 DASFSW010000000
VERSION     DASFSW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.063"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            40..711
                     /locus_tag="U1099_001217"
     CDS             40..711
                     /locus_tag="U1099_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEM4867537.1"
                     /translation="MDFNWEEFLDPYIQTVGELKIKLRGIRKQYRKANRHSPIEFVTG
                     RVKPIESIKEKMALRHIKLENLAQDMQDIAGLRIMVQFVDDIEEVLAILRKRKDMQIV
                     HERDYINNMKASGYRSYHVVIEYPVDTINGNETVLAEIQIRTLSMNFWATIEHSLNYK
                     YKGNFPEEIKKRLEVTAKIAYELDEEMRKIRNDIQEAQALFDPAYRKLNDGVGNSDDT
                     DEEYR"
     gene            686..1513
                     /locus_tag="U1099_001218"
     CDS             686..1513
                     /locus_tag="U1099_001218"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEM4867538.1"
                     /translation="MIQMKNTGRKKIALLASRNPKSEAVSKELWTKLKEANFILTPKN
                     PDIVISIGGDGMLLSAFHKYEKLIDRVRFVGIHTGHLGFYTDYRDFEVDKLIENLKLD
                     TGARVSYPILNVKVKMTDGRIVEARALNEATVKRLSKTMVADIIINNVPFERFRGDGI
                     SVSTPTGSTAYNKSLGGAVLHPTIEALQIAEVASLNNRVYRTLGSSVVVPKKDKIVIE
                     PKHSDRYSIAVDNKTFVYDSIESIEYQIDNSKIHFVATPSHTSFWNRVKDAFIGEVE"
     gene            1513..2406
                     /locus_tag="U1099_001219"
     CDS             1513..2406
                     /locus_tag="U1099_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM4867539.1"
                     /translation="MRFEFIADQHTKIKTFLKKHGVSKGLLAKIKYTGGNIWVNDIEQ
                     NATYLLDIGDRVTIDIPAEEDLTGSLKPIFFPLDIVYEDDHFLSINKPVGYASIPSAL
                     HSSTIANFVKGYLIEQNYENKQVHIVTRLDRDTSGVMLFAKHGYAHARLDKQLQAKLI
                     HKRYYALVKGNGQLEETGDIIAPIGRPEDSIITRCVTKNGKYAHTSYRVVQSWGDIHL
                     VDIQLHTGRTHQIRVHFSHIGFPLLGDDMYGGSLECGIERQALHCHNLAFDNPFSAER
                     IDLEAPLPEDFQAVINQLISK"
     gene            2424..3398
                     /gene="pta"
                     /locus_tag="U1099_001220"
     CDS             2424..3398
                     /gene="pta"
                     /locus_tag="U1099_001220"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEM4867540.1"
                     /translation="MKIFEQLATKLEGKKVRIVLPEGEEPRILQATKRLVNESDVVPV
                     LLGNPDRIKIYLDIEGITEGFEIIDPAHYDRYDEMVAALVERRKGKVSEEQAHELLKD
                     VNYFGVMLVYMGVVEGMVSGAIHSTASTVRPALQIIKTEPGVSKTSGAFLMVKGEERY
                     IFGDCAINIDPDAQTLAEIAINSARTAKMFGIDPKVAMLSYSTKGSGAGPKVDKVVEA
                     TKIAQKLRPELDLDGELQFDAAFVPTTGKLKAPGSSVAGQATVFIFPGISAGNIGYKI
                     AERMGGFAAVGPVLQGLNQPVNDLSRGCNPDDVYNLTLITAVQALEHR"
     gene            3400..5136
                     /locus_tag="U1099_001221"
     CDS             3400..5136
                     /locus_tag="U1099_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867541.1"
                     /translation="MKFLSRYFKDYIKESILGPVFKLLEACFELLVPLIIAYIVDTII
                     PNGSQGNLVAMLFLLVGLACIGIIVSLIAQYYSAKAAVGVTKELTNDLYQKVLSLPKS
                     SRDILSSSSLLTRLTSDTLQIQTGINTFLRLFLRAPIVVFGSLIMAFYISPSLSAYFL
                     EMIILLIFIVTVISVMTSRIYQSMRKELDGLVGQVRETVTGWRVIRAFGQREREIKAF
                     QGINQIYKKQQLQAGFWSSLLSPLTFLVVNGTLLILIWQGNIAISHNLLEQGMLVALI
                     NYLLQILVELVKMIMVVSTLNQTYISAQRIQEVFDQTSEDVESSLPKVVSEDKEIIFS
                     VRHISFSYPKSAEESLSDIAFDLRKGQFMGIIGGTGSGKSTLVDLLQALYSVPTNQLS
                     LFIDGKSPKNLKEWRQQIAIVPQQAQLFAGTVRSNLSLGLESVLDSELWAALEIAQAK
                     SFIEDKGGLDSPVEAFGKNFSGGQRQRLTIARAILQKAPILVLDDATSALDYLTESRL
                     LAAIRRAFPAQTLIMVSQRTNSLRTADQILVFDQGRQVGLGRHEDLLRSSAIYQEIDQ
                     SQHREEDSHETI"
     gene            5123..6865
                     /locus_tag="U1099_001222"
     CDS             5123..6865
                     /locus_tag="U1099_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867542.1"
                     /translation="MKQSSFKELVRLVLGQPFQMYLMLVGTLVQVLLTVYLPILIGQA
                     VDAVLIADGQVLLGIIGKMVMVILLNTLVQWYLPLVTNRLVYGMVADLREQVYVKLHQ
                     MPLSYLDRHSVGDLVARFSSDSEQLTNGLLMVFNQFFIGILTIFLTIFTMARLDLTMM
                     LLVVALTPVSLLVARYIAKKSYGYYRKQTQARGKQSQLLEESISQLTLVQSFNAQEQF
                     TQGFQVVNDQYATYSQQAIFASSTVNPSTRFINAIIYALLAGLGALRIMAGQFTVGSL
                     TTFLNYASQYSKPFNDISSVLSELQSALACADRLFAILALDSIDDQAERKIDSEELKG
                     AISFDNVSFSYSPERSLIEHLDIDVEAGQKVAIVGPTGAGKSTMINLLMRFYDVTAGQ
                     IVLDGVPIRQYSREDLRRQIGMVLQETWIKSGTIHDNIAYGYPNATRELVIEAAKAAN
                     ADFFIRQLPQGYDTVLADGGEGLSQGQRQLLSIARVFVKIPKILILDEATSSIDTRTE
                     ILVQEAFAKLMEGRTSFIIAHRLSTIQSADLILVMVDGKIVEQGNHEALMAEQGVYYQ
                     MQTSQQTEEDESVR"
     gene            6849..7241
                     /locus_tag="U1099_001223"
     CDS             6849..7241
                     /locus_tag="U1099_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEM4867543.1"
                     /translation="MKVLDKLNLQPITVLDDYQATMLNEKEVLLTTKVTEKSLNPYGM
                     AHGGFLFTLADSVAGLTTVAGGSYSVTLQSNIHYMKAAKLGDTLSVTGSCTHDGSRTK
                     VVEVKIKNQDKQLLASASFTMFVTGKVE"
     gene            7476..8636
                     /gene="mutY"
                     /locus_tag="U1099_001224"
     CDS             7476..8636
                     /gene="mutY"
                     /locus_tag="U1099_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEM4867544.1"
                     /translation="MLDLKEYGIEMWPEEKIQAFRKALLDWYDANKRDLPWRRTKDPY
                     AIWVSEIMLQQTRVDTVIPYYERFLHYLPTISDLAQAPEEVILKLWEGLGYYSRVRNM
                     QKAAQQMVEDFDGQFPTTHAAISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLSR
                     LFEVDYDIGLPANRKIFQAMMELLIDPERPGDFNQALMDLGSDIESPVNPRPQDSPVK
                     AFSAAYLNGTMDKYPIKAPKKKPIPVAYQGFLIRNKDNQFLLEKNNEAGLLSGFWSFP
                     LLEKGAIVEKQVSLFEVAEEVVQPDIRQSFTELYGLTVDWQEQEFGIVQHIFSHRKWQ
                     IEMVEGVAETLNLPESKELKWVSVEDFPTYPFAKPQQKMWENFIKEKEKQKS"
     gene            complement(9299..10099)
                     /locus_tag="U1099_001225"
     CDS             complement(9299..10099)
                     /locus_tag="U1099_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867545.1"
                     /translation="MILGDILKEYRSKNKLSMDKFAELSGLTKGYISMLEKNQHPKTK
                     KALLPTMDTLEKVAKGMAISVGELIEQLDSNQDIALTITPSQFKLFQQPIHPEVQTLD
                     NELKGDFHAQWLTFGQEQLAAMQTTSITPSASILHLDDYRERIDLPVPGKVSAGTGYW
                     QDTDMNNLVSFYNDEIPDNQSYDTIAQVVGDSMAPHIEDGDYLFIRLTPHIPLNTIGI
                     FSVNGENFVKKFKGDYLQSLNSDYDDIYFSPEDDIRPIGQVVDIYSPD"
     gene            10231..10701
                     /locus_tag="U1099_001226"
     CDS             10231..10701
                     /locus_tag="U1099_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867546.1"
                     /translation="MKRMTEISWNDIYKEWETYANHFGLTTPINTENLRDQKSKDFGK
                     GSLITLDLLADYDTDSEKTAAIWVASFCRDLIQDYAYLLNGRAYLTVNQIYFQALKQF
                     QSGAVIWSKPLTRLQPKLFVSYRLLENLDLSHYSCVVELAMLQASMVRTQILEK"
     gene            10721..11263
                     /locus_tag="U1099_001227"
     CDS             10721..11263
                     /locus_tag="U1099_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867547.1"
                     /translation="MFNLLNKKAEVSKVAEYWNDTLIERGILSADELLEGKCWRCKSS
                     HGVAVCQIVSSKWSKDASLANQMVLCLSCQHEKPNVADTEIVWQWLEVENDERYWTLQ
                     GMAEYEKMYKKSVLQELWDMGIRDGEEVETLVNKVTSLSRKNDIVLNRATLAGLFRCE
                     IEQMRRKAFLNWTGIFKLVS"
BASE COUNT         3446 a         2034 c         2568 g         3480 t
ORIGIN      
        1 tctaatacaa tttaaaagtt ttgacaagaa aggggccgta tggactttaa ctgggaagaa
       61 tttttagacc cctatattca gacagttggt gaattgaaaa tcaaactgcg ggggattcgc
      121 aagcagtatc gcaaggctaa tcggcattct ccaattgagt ttgtcacagg acgggtaaaa
      181 ccgattgaat ccattaaaga aaaaatggct ctgcgacata ttaaattgga aaatcttgct
      241 caagatatgc aagatattgc cggtcttcgg attatggttc aatttgtgga cgacattgaa
      301 gaagtattag ctattttgcg gaaacgcaag gatatgcaaa ttgtgcatga acgggattat
      361 atcaataata tgaaagcctc tggctaccgt tcatatcatg tggtgattga gtatcctgtg
      421 gatactataa atggcaacga gacggttctg gcagaaatcc agattcgaac cctttccatg
      481 aatttctggg ctacaattga acattctctg aattataaat ataagggaaa ttttccagaa
      541 gaaatcaaga aacgcttgga agttacagca aaaattgcct atgaattaga tgaggagatg
      601 cgaaaaatcc gcaacgacat tcaagaagcg caggccttat ttgatcctgc ctatcgcaaa
      661 ctgaatgacg gtgtgggcaa cagtgatgat acagatgaag aatacaggta gaaaaaaaat
      721 cgctctgctc gctagtcgaa atcctaagag tgaagcggtg tccaaagaat tatggacaaa
      781 gttaaaagaa gcaaatttta ttttgactcc taaaaatcca gatattgtca tttctatcgg
      841 tggagatggg atgcttttat ctgcttttca caaatatgaa aagttgattg atcgtgtacg
      901 ttttgtagga attcacacgg gtcatttggg gttttataca gattatcgcg attttgaagt
      961 ggacaagctg attgaaaatc ttaagcttga tacaggtgct agagtatcgt atccgatttt
     1021 aaatgttaag gtcaagatga cagatggtcg cattgtcgag gcgcgtgcct taaatgaggc
     1081 gacagtaaaa cgtctttcta agaccatggt cgcagatatt attattaaca atgtgccctt
     1141 cgaacgcttc agaggggatg gaatttcggt ttcaacgccg acaggctcaa cagcctataa
     1201 caagtcatta ggtggtgctg ttttacatcc aacaatcgag gcattgcaga ttgcagaagt
     1261 ggcaagtttg aacaatcgtg tttaccgcac cttgggttca tccgttgttg tccctaaaaa
     1321 agacaagatt gtgattgaac caaagcatag tgaccgttac tcgattgcgg tagacaataa
     1381 gacttttgtc tatgatagta ttgaaagtat agagtatcag atagacaata gtaagattca
     1441 ttttgttgca acaccgagtc atactagttt ttggaatcgg gttaaggacg cctttattgg
     1501 agaggtggag taatgcggtt tgaattcatt gctgaccagc atacgaaaat aaagactttc
     1561 cttaaaaaac atggtgtctc taaaggacta ttagcgaaga ttaagtatac cggtggcaat
     1621 atctgggtca acgatattga gcaaaatgca acttacttgc ttgatattgg agatagggtt
     1681 acaattgaca tacctgctga agaggatttg actggtagtt tgaagccgat tttctttccg
     1741 ttggatattg tctatgaaga tgatcatttt ctgtcgataa ataagccggt tggctacgcc
     1801 tccattcctt ctgccttgca ttcatccaca atcgcaaatt ttgtaaaagg ttatcttatt
     1861 gagcagaatt atgagaataa gcaggttcat attgtgactc gtctggatag ggatacatca
     1921 ggcgtcatgc tgtttgccaa gcacggctat gcccatgcac gactcgataa gcagttacag
     1981 gccaagctta ttcataaacg ttattatgct ttggtgaagg ggaatgggca gctagaagag
     2041 acaggggata ttattgcccc tatcggtcgt ccagaagata gcatcattac ccgttgtgtg
     2101 acaaagaatg gaaaatatgc ccatacttcc tatcgtgttg tgcaatcgtg gggagatatt
     2161 catttagtag atattcaact acatactggg cggactcatc agattcgtgt tcatttttct
     2221 cacattggat ttccgttgtt aggagatgat atgtatggtg gaagtttgga atgtgggata
     2281 gaacgtcaag ccttacattg tcataatttg gccttcgaca atcctttttc agccgaaagg
     2341 attgatttgg aagcaccgct tcctgaggat tttcaggctg ttatcaatca gttaataagt
     2401 aaataaattt aaggagtttg ttcatgaaaa tttttgagca attagctaca aagctagagg
     2461 gtaaaaaagt acgtatcgta ttgccagaag gtgaagaacc acgtatcttg caggcgacaa
     2521 aacgtttggt aaatgagtcg gatgtagtac ccgtcttgct tggaaaccca gatcgtatta
     2581 agatttacct tgacattgaa gggattacag aaggttttga aattattgac ccagctcatt
     2641 atgatagata tgatgagatg gtagctgctt tggtagagcg ccgtaaaggt aaggtatcag
     2701 aagagcaggc acatgagttg ttgaaagatg ttaactactt tggtgttatg ttggtatata
     2761 tgggcgtagt agaaggtatg gtatctggtg cgattcattc aaccgcttca acagttcgtc
     2821 cagctcttca aatcattaaa acagagccag gtgtatcgaa aacatcaggt gccttcttga
     2881 tggtaaaagg tgaagagcgt tatatttttg gtgactgtgc tatcaacatt gatccagatg
     2941 ctcaaacctt ggcagaaatc gctatcaact cagcacgtac tgctaaaatg tttggtattg
     3001 atcccaaagt agcgatgctc agctattcta caaaaggctc tggtgctggt cctaaagtag
     3061 ataaggttgt tgaagctaca aaaattgctc aaaaattgcg tcctgagttg gatttggacg
     3121 gtgaattgca atttgatgct gcctttgttc ctaccacagg taaattaaaa gccccaggtt
     3181 cgagtgttgc tggtcaagcg actgtattta tcttcccagg tatctcagct ggtaatattg
     3241 gttataaaat tgccgaacgt atgggtggat ttgcagcggt aggtcctgtt ttacaaggtt
     3301 tgaatcagcc tgtaaacgac ttgtctcgtg gttgtaaccc tgatgatgtt tacaacttga
     3361 ccttgattac tgcggttcag gctttggagc accgttaata tgaaattttt atcacgatat
     3421 tttaaggact atattaagga atccatatta ggtccagttt ttaaactatt ggaagcttgt
     3481 tttgagcttt tggtaccatt aattatcgcc tatatcgttg atacaattat tccaaatggc
     3541 agccagggaa acctggttgc tatgcttttt ttactggttg gtctagcatg tattggaatt
     3601 attgtttcac taatagcgca atattattca gctaaagcag cggttggagt gactaaagag
     3661 ctgacaaatg acttgtatca gaaggttctt tcccttccca agtctagccg agatattctt
     3721 tcctcatcca gcttgctgac acgattgacc agcgacactt tacaaattca aacaggtatt
     3781 aatacttttc ttcgtttgtt tttacgtgct ccaattgtcg tatttggttc gctcatcatg
     3841 gcattctata ttagcccaag tctatctgcc tattttctag aaatgattat cctattgatt
     3901 tttatagtga cagtcatttc tgttatgacc agccgtattt atcaatcgat gcgaaaagaa
     3961 ttagatggtt tggtggggca ggtacgtgag acagtgactg gttggcgagt gattcgtgca
     4021 tttggacaaa gagagcggga aattaaggct tttcaaggca taaatcagat ttataagaag
     4081 caacagttgc aggctggttt ttggtctagt cttttatcgc cattgacatt tttagtggtc
     4141 aacggcacct tgctcattct catctggcag ggaaatatag ccatttcaca caatttgttg
     4201 gaacaaggga tgttggtcgc tctcatcaac taccttttac aaattttagt agaattagtc
     4261 aagatgatta tggtcgtgtc tactctcaac cagacctata tttcagcgca acgtattcaa
     4321 gaggtttttg accaaacatc tgaggatgta gagtctagtt tgccaaaagt ggttagtgag
     4381 gataaggaaa tcatcttttc tgtgcgtcat atttcctttt cttatccaaa atctgctgag
     4441 gagtccctgt ctgacattgc ttttgacttg cgtaagggac agtttatggg aattatcggg
     4501 ggaactgggt caggtaagtc caccctagta gatttattgc aggcactcta ttcagtgcca
     4561 acaaatcagc tctctctctt cattgatgga aaaagtccta agaatttaaa agaatggcgg
     4621 cagcaaattg ccattgttcc gcaacaggct cagctttttg ctgggactgt gcgttctaac
     4681 ctgtcattag gcttagaaag tgtattggac agtgagttat gggcagcgct agaaatcgca
     4741 caggctaagt ccttcattga ggacaaagga ggcttggata gtcctgtcga agcctttggg
     4801 aagaactttt ctggtggaca gcgtcaacgt ttgaccattg cacgcgctat tttacagaaa
     4861 gcaccgatat tggttttaga tgacgcgacc tctgctttgg attatctgac agagagtcgg
     4921 ttattggccg cgattcgtcg tgcatttcct gctcaaacct taataatggt atctcagaga
     4981 accaatagtt tacgaacagc tgatcagatt ttggtctttg atcaaggccg tcaagtggga
     5041 cttggccgtc atgaagactt gctgagaagc tctgccattt accaagaaat cgatcaatca
     5101 caacataggg aggaggacag ccatgaaaca atctagtttt aaggaactgg tcagattggt
     5161 tttaggccaa ccctttcaaa tgtacttgat gttggtggga actttagtgc aggtgcttct
     5221 gacagtttat ctgcctatct tgattggtca ggcagttgat gcagtattga tagcggatgg
     5281 tcaggtcttg ctagggataa ttggcaaaat ggtgatggtt atcctgctta atacgctggt
     5341 gcaatggtat ctcccgctag taaccaatcg cttggtttat ggaatggtgg ctgacttgcg
     5401 tgaacaggtt tatgttaagc tgcaccagat gcccctatct tatcttgatc gtcattcagt
     5461 tggtgattta gtagctcgtt tttcaagtga tagtgagcaa ttaaccaacg gactcttaat
     5521 ggttttcaat caatttttca taggtatctt gaccattttc ctaaccattt tcactatggc
     5581 taggcttgac ttgacgatga tgttgctggt tgtggcattg actccagttt ctctgcttgt
     5641 tgcacgctat atcgctaaga aatcctatgg ttattataga aaacaaacac aggcacgtgg
     5701 gaagcaatct cagctacttg aggaatcgat cagtcaattg accttggttc aatctttcaa
     5761 tgcccaagag caatttacgc aaggttttca agttgtgaat gaccagtatg cgacctattc
     5821 ccaacaggct atctttgcct cttcaacggt caatccaagc acgcggttta tcaatgctat
     5881 tatttatgcc ttacttgcag gacttggagc gctgagaatt atggcagggc agtttacagt
     5941 gggttcactg acaacttttc tcaattatgc tagtcagtac agcaagccct ttaatgatat
     6001 ttcctcggtc ctatcggaat tgcagagcgc cttagcttgt gcagacaggc tgtttgctat
     6061 cttggcttta gatagcattg acgatcaggc tgagcggaaa attgattcag aggagttgaa
     6121 gggagctatt tcctttgaca atgtgtcttt ctcatatagc cctgaacgtt ctctgataga
     6181 acatttggac attgatgtcg aagctggaca aaaggtagcc attgttggac ctactggtgc
     6241 cggtaaatca accatgatta acctgcttat gcgcttctac gatgtgacgg ctggtcagat
     6301 tgttttagat ggggttccga ttagacagta cagtcgtgag gatcttcgca gacagattgg
     6361 gatggtttta caggagacat ggatcaaatc tggtaccatt catgataata ttgcctatgg
     6421 ctatccaaat gccacgcggg aattggtcat tgaggcggct aaggcagcta atgcagactt
     6481 ctttattcgt cagcttccgc aagggtatga tacagttttg gcagatggag gagaaggctt
     6541 atcccaaggg caacgtcagc tcttgtctat tgcgcgtgtc tttgtcaaga tacctaaaat
     6601 cctgatttta gatgaggcga cttcctctat tgatacacgg acagaaattt tagtacaaga
     6661 agcatttgcc aaattgatgg aaggacggac cagctttatt attgctcatc gcctatcaac
     6721 cattcaatca gctgacttga ttttggtcat ggtggatgga aaaattgttg aacaagggaa
     6781 tcatgaagcg ttgatggctg aacaaggcgt ttattatcaa atgcaaacaa gtcagcagac
     6841 agaggaggat gaaagtgtta gataaattaa acttacaacc gattacggtt ttagatgact
     6901 atcaagctac aatgctcaat gagaaagagg tgctactgac cacaaaggtg acggaaaaat
     6961 ctctcaatcc atatggtatg gctcatggag gcttcctctt tactttagca gattcagtag
     7021 caggattgac aacagtggct gggggttctt attctgtcac cctgcagtcc aatatccatt
     7081 atatgaaggc ggcaaaactt ggtgatacct tatccgtcac tggttcttgc acacatgatg
     7141 gaagtagaac aaaggtcgtt gaagtgaaga taaaaaatca agataaacag ctacttgctt
     7201 cagcaagctt taccatgttt gtaacaggaa aagtagaata aaactcttcg aacatcaaaa
     7261 gcagacagtc ttggctagat tgagagaaaa aaacttcgtt cgctctattt ccaacctccc
     7321 aagtttcccc aaacctttgt agaaatgaac ttcgttcgct caatttccaa cctccaaagg
     7381 ttccccaaac ctttggagct agtttgattt tgattataaa cgatataagg gagttggcaa
     7441 aagctggctc cctttctttg tgttatacta gatgtatgtt agatttgaaa gaatatggaa
     7501 tcgaaatgtg gccggaggaa aagattcaag cttttcgcaa ggcactctta gattggtatg
     7561 atgccaataa acgtgacttg ccttggagac ggaccaagga tccgtatgct atatgggttt
     7621 ctgaaatcat gcttcaacag acaagagtag acacggtcat tccttattat gaacgttttc
     7681 tccattactt gcctacgatt agtgatttag cacaagcacc agaggaggtc attctcaaac
     7741 tctgggaagg attgggctat tattcgcgcg tgcggaatat gcaaaaggca gctcagcaaa
     7801 tggtagaaga ttttgatggg caatttccga ctacgcatgc agccatttca agcctaaagg
     7861 gcattggtcc ctatactgct ggagcaattt ccagtattgc ttttaatttg ccagaaccag
     7921 cggtagatgg caatgtgatg cgggttctca gccgcttgtt tgaggttgat tatgacattg
     7981 gtcttcctgc caatcgtaag attttccaag cgatgatgga gctattgatt gatccagagc
     8041 gaccgggcga tttcaaccaa gccctaatgg atctagggtc ggatattgaa tcgccagtta
     8101 atccacgtcc acaggacagc ccagtcaaag cctttagcgc agcctactta aacggtacaa
     8161 tggataagta tccaatcaaa gcaccaaaga aaaagccgat tcccgtggcc taccagggat
     8221 ttctcatcag gaataaagac aatcaatttt tattggaaaa aaataatgaa gcaggtcttc
     8281 tatcaggttt ctggtctttt ccgcttttgg aaaaaggagc aatcgttgaa aaacaagtct
     8341 ctctctttga agttgcagaa gaagttgttc agccagatat tcggcagagc tttacggaac
     8401 tttatggtct gactgttgat tggcaagagc aagaatttgg catcgtccag cacattttta
     8461 gtcaccgcaa atggcagata gaaatggtag aaggtgtggc agaaacactt aaccttccag
     8521 aatcaaaaga attgaaatgg gtttctgtag aagattttcc tacctaccca tttgctaagc
     8581 cgcaacaaaa aatgtgggaa aactttatca aggaaaaaga aaagcaaaaa agttgacttt
     8641 ggagcacata attatttgaa agcgttttac aatagtggta taatctaatt gaaaaaagga
     8701 aatagttagt taagatgttg aacgccatct gaattgctga aaggagttct ttagcgttcg
     8761 tagaagtgta tatactatta gccatctttc tctaataaaa agagagggat gctagtaggg
     8821 gaaaagtccg ttatccctgt tttatatcta tttgtgatat ccattgtcaa atgcatagca
     8881 ttaactcatt atttagcctt gctacccggt tcaatcagaa gtaggctaag attattcgtg
     8941 tcgtaactac tatgtgttca agatgttgta acttttgaac ataactttag aaaatcacaa
     9001 aaacctctag cgattttcat cataataagc aatctccaca ctttagacaa atagtaagag
     9061 gacaatcaaa gacataaggg tagcagaata attatttaat cattaacttg ttgtcaaata
     9121 actatctggt aatggacttt tccaatttga catataaaag ggaggctgtc atagcctcct
     9181 tttactgtgc ttttcagttt gttttcagta ggaaaaaacg ccagctatac gaacagactt
     9241 tccaaaacta gatggatgaa tgtagccaat ataactagtg attaactagg cttagaaatc
     9301 aatctggact ataaatatct acaacttgac caataggtcg aatatcgtct tctgggctaa
     9361 aatagatatc atcatagtca gaattgagtg attgcaaata gtcacctttg aactttttaa
     9421 caaaattttc cccattgact gaaaaaattc caatcgtatt tagaggaata tgaggagtta
     9481 ggcgaatgaa gagatagtct ccatcctcga tatgcggagc catagaatct cccacaacct
     9541 gagcgattgt atcataagat tggttatctg gaatctcatc gttatagaat gatacaagat
     9601 tgttcatatc tgtatcctgc cagtaacctg taccggcaga aaccttacca ggaacaggta
     9661 aatcgattcg ctctcgatag tcatccaagt gaagaataga agcagaagga gtaattgagg
     9721 tagtttgcat agctgctaac tgttcttgac caaaagttaa ccattgtgca tggaaatcac
     9781 cttttaattc attgtccaag gtttgaacct ctggatgaat aggctgttga aagagtttaa
     9841 actgggaagg agtaatcgtt aaagcaatat cctgattaga atctagttgc tcgattagct
     9901 caccaaccga tatagccatg cccttagcga ctttttctag cgtatccatc gttggcagga
     9961 gagctttttt ggttttggga tgctggttct tttccaacat agaaatataa ccttttgtta
    10021 aaccagataa ttcagcgaat ttatccattg ataatttatt cttgcttcgg tattctttta
    10081 aaatatcacc taaaatcatg gttgtctcct tatctgttta accaattata ccattaaaaa
    10141 atattttttg caaattttgt ttaacacgtt tgacaaaaaa tcaaaacggt gttatgataa
    10201 tctcacaaaa aagaaaaata ggagtcgcaa atgaaaagga tgacagagat ttcttggaat
    10261 gatatttata aagagtggga aacctatgct aatcattttg gtttgacgac tccgataaat
    10321 actgaaaatt tgcgagatca gaaatcgaag gattttggaa aaggaagtct gattacgctg
    10381 gacttattag ccgattacga tactgattct gagaagaccg cagctatttg ggtagcttca
    10441 ttttgtagag atttaataca agattacgca tatttactaa atggtagagc ctatcttaca
    10501 gtcaatcaga tttattttca ggcactcaaa caatttcagt caggagcggt aatatggtca
    10561 aaacctctca cacgattgca accgaagtta tttgtatctt atcgtttgtt ggaaaatctt
    10621 gatttgagtc attattcttg tgtagttgag ttagcaatgt tgcaggctag tatggttcgg
    10681 acacagatat tagaaaagta attatagtag gagaatgaaa atgtttaatt tgttgaataa
    10741 gaaagctgaa gtatcgaaag ttgcagagta ttggaatgat actttgatag aaaggggaat
    10801 tttgagcgct gatgaattgc ttgaaggaaa atgctggaga tgtaaaagtt ctcatggtgt
    10861 agctgtgtgt cagattgtat caagcaaatg gagcaaggat gcgagcctgg ctaatcagat
    10921 ggtcctctgt ctatcctgcc agcatgaaaa acctaatgta gctgatacag aaattgtgtg
    10981 gcaatggtta gaagttgaga atgatgaacg ttactggact ctacaaggta tggcagaata
    11041 tgagaaaatg tataaaaaat cagtacttca agagttatgg gatatgggaa ttagagatgg
    11101 tgaagaagtc gagacgcttg taaataaagt aactagcctt tctcgtaaga atgatattgt
    11161 tttaaataga gcaaccttag caggattatt tcgctgtgaa attgaacaaa tgcgtcgaaa
    11221 ggcatttcta aattggacag gaatttttaa gctagtcagt taactgatgc agttgtagga
    11281 aaacatctca tgctgattgg agtaacagat aaataaaaag cgcatcagct tagaatctta
    11341 aagaaagaag tctaatgatg cgtttatagg attatatttc agctaataaa aattggagtg
    11401 aatgtttttg aattatagct caaaaacgtt agtcaaaacc ttattatacc aacgattatg
    11461 gcgctttttt ggtgccagtt ggtgttaatt ataatgataa gtcgtttaag agacttaaag
    11521 catcggca
//
LOCUS       DASFSW010000064        11515 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.064, whole genome shotgun sequence.
ACCESSION   DASFSW010000064 DASFSW010000000
VERSION     DASFSW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.064"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            24..1349
                     /locus_tag="U1099_001228"
     CDS             24..1349
                     /locus_tag="U1099_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867548.1"
                     /translation="MCYNHLMKKISDLGLTGRKLVGEGLILVFIGIGFLIAGWQFPGL
                     ILRFVHAGLFFLALYELSMTFFRKKKSSESVIALVGKAVLFGILASLDLAVQIPLYFA
                     AIFVGIYQLFTALINFITFYLYRKDGVQPRIRYLIDGIWLSLLGSASLFVSGTQLVVQ
                     TIVIGGYLILYGLTNLRDGFLFEEAIEQQSLKRHVRLTLPLFLAALVPRMTLQKVNDY
                     LADNEGQTAQSIYNRHKEIAEFPALEVFVHVGEEGFGAVGHVDLSYKGQVYGFGSYDV
                     LSERLGGAIGDGVLFRAERQAYIDFCNQEGMTMLGYQLALSSEQEKAVETRLAEIEGL
                     LLPWQPSAEKVSRRSDGQPIEMYAYRMKEAIGAVLFKFKKSKFKTYFVLSTNCVLLAD
                     SVIGQAGTDVLGLRGFIAPGTYQSYLDQEYEKPHSLVVAKNIYYRKEKS"
     gene            1425..4061
                     /gene="polA"
                     /locus_tag="U1099_001229"
     CDS             1425..4061
                     /gene="polA"
                     /locus_tag="U1099_001229"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEM4867549.1"
                     /translation="MKKNKLLLIDGSSVAFRAFFALYNQIDRFKSPTGLHTNAIYGFN
                     LMLDHMMKRIEPTHILVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFPFIRQMLDA
                     MGVKHYELAQYEADDIIGTLDKMAERTEIPFDVTIVSGDKDLIQLTDENTVVEISKKG
                     VAEFEEFTPAYLMEKMGITPTQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLTEFGSL
                     DGIYENIDSMKASKMKENLIADKEKAFLSRTLATIDTQAPIEIGLDDIVYQGPKVDEL
                     GQFYDDMGFKQLRAQLGTTSSQEEAVLDFQIVTEISPAMLKQDQFFYFEILGENYHRE
                     DLVGLAWGDKEKIYVGGPELLDSPVLRDFLENQTIKTYDFKRGKVLLDRKEITLPPAT
                     FDSRLAKYLLSTVEDNSLTTIANLYGQISLVPDEAVYGKGAKLDLPEREIFFPHLARK
                     VQVLIETEEPMLTKLEENQQLDLLFDMELPLANVLAKMEIAGIKVEAETLKAMQSENE
                     VLIDQLTKEIYELAGQEFNINSPKQLGTILFEEMGLPLEYTKKTKTGYSTAVDVLERL
                     APIAPVVSKILEYRQITKLQSTYVVGLQDAILEDGKIHTRYVQDLTQTGRLSSTDPNL
                     QNIPVRLEQGRLIRKAFVPSLENSVLLASDYSQIELRVLAHISQDKHLIEAFQQGADI
                     HTSTAMRVFGIEKAEDVTPNDRRNAKAVNFGVVYGISDFGLSNNLGITRKEAKAYIDT
                     YFERFPGIKNYMETIVREARDKGYVETIYKRRRELPDINSRNFNVRNFAERTAINSPI
                     QGSAADILKVAMINLDKALTEAGLATRMLLQVHDEIVLEVPVAELETVKAMVKETMES
                     AISLSVPLIADENEGSTWYEAK"
     gene            4048..4830
                     /locus_tag="U1099_001230"
     CDS             4048..4830
                     /locus_tag="U1099_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEM4867550.1"
                     /translation="MKQSKKGKSMKKLILCIGLCSLLLAGCGKASQKTEKTPASSGEE
                     FSTTLPILQEKQDTTNEQFNVLANAEVVAVNSTPPADETQKGHKIYAANNGVAEKDEN
                     GNIKEYFRYYNVPSTWTINAENTKDETVAAVYDVKEGGSNYMVQLYNINAFNQSPLED
                     GRNMTPEELEARMVETNHSFNEKTVVTINGQEWRVGRQLLTDQKLARLTFYRMESTAA
                     YDDSVVVGSIYYSLDPGTDKDRTNLKKVIGQVKDVVYQIAKK"
     gene            4950..5387
                     /locus_tag="U1099_001231"
     CDS             4950..5387
                     /locus_tag="U1099_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEM4867551.1"
                     /translation="MSYSFQNPSQDIIFDYLKNAKTIAVVGLSSREETAAYRVSKLMQ
                     EAGYKIIPVNPKAAGGTILGELVYSSLAEIDQPIDIVDVFRRSEFLPEVAQEFIQSNA
                     KIFWAQLGLESQEAEKLLRQAGRNDIVMNKCIKIEYLEMKEQY"
     gene            complement(5682..6524)
                     /locus_tag="U1099_001232"
     CDS             complement(5682..6524)
                     /locus_tag="U1099_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM4867552.1"
                     /translation="MFTISNIRERARQTLNETPGIYQVAVIPVTISVIVQLISFSRNS
                     LAGVSAEELASPSYFISTSLFPVFYGLLLGMLSLSVIWTIFNIIKGGKTTTSFKDSLS
                     IFNHKDFWKIFRTYILKSFLLFLWGLLFYLGIGLLIGAGTMITIIAASSQSLDPDALP
                     MEILAILGLMAMGGILLMVLGTALYIPQIYAYSQVEYILFEQLEREEYTDAFSIIRSS
                     RKLMKGYKFKRFTLDLSFIGWFLLVIITFGLAGLYVWPYHYAAQMHFHEEILDDQAKK
                     MSYV"
     gene            6978..8120
                     /gene="tgt"
                     /locus_tag="U1099_001233"
     CDS             6978..8120
                     /gene="tgt"
                     /locus_tag="U1099_001233"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEM4867553.1"
                     /translation="MTDVPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTMSPEELKAMGSGIILSNTYHLWLRPGDELVARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNISEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSRGRLVVKNAQFAEDFTPLDPECDCYTCKNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLINLMKNVRQAIMDDNLLEFRQDFMEKYGYGKNGRNF"
     gene            complement(8453..9538)
                     /locus_tag="U1099_001234"
     CDS             complement(8453..9538)
                     /locus_tag="U1099_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM4867554.1"
                     /translation="MSKLHHVKSYLEANKMDLAIFSDPVSIYYLTGYHSDPHERHMML
                     FVMPDHDSLLFLPALDVERAVATVDFPVAGYMDSENPWQIIKSKLPQKSFSVICAEFD
                     NLNLTRYHGLQSIFSQPFSDITPLINTMKLIKSRDEIEKMLVAGEFADKAMQVGFNNI
                     SLNVTETDIIAQIEFEMKKQGISKMSFDTMVLTGDNAANPHGIPSTNKIENNALLLFD
                     LGVEALGYTSDMTRTVAVGKPDQFKKDIYNLTLEAHMAAVNMIKPGVTAGEIDYAARS
                     VIEKAGYGEYFNHRLGHGLGMSVHEFPSIMEGNDLVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGYVTKNGFEVFTKTPKELLYFEG"
     gene            9748..10752
                     /gene="ccpA"
                     /locus_tag="U1099_001235"
     CDS             9748..10752
                     /gene="ccpA"
                     /locus_tag="U1099_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEM4867555.1"
                     /translation="MLNTDDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLE
                     VIDRLDYRPNAVARGLASKKTTTVGVVIPNIANAYFATLAKGIDDIADMYKYNIVLAN
                     SDENDEKEINVVNTLFSKQVDGIIFMGYHLTDKIRAEFSRSRTPIVLAGTVDLEHQLP
                     SVNIDYAAASVDAVNLLAKNNKKIAFVSGPLVDDINGKVRFAGYKQGLKDNGIEFNEG
                     LVFESKYKYEEGYALAERILNAGATAAYVAEDEIAAGLLNGVSDMGVKVPEDFEIITS
                     DDSLVTKFTRPNLTSINQPLYDIGAIAMRMLTKIMHKEELENREVVLNHGIKVRKSTK
                     "
     gene            complement(11184..>11515)
                     /locus_tag="U1099_001236"
     CDS             complement(11184..>11515)
                     /locus_tag="U1099_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4867556.1"
                     /translation="VETSEKQMPVNEKDNAVTPERQIANKEKENIETSKKQIPVNGNN
                     QNGTVGENSNTKPTTEKTDKQETSTFKTETAKQILPVTGEKGSLWLLTSGIIGLAIAL
                     FTRKRKL"
BASE COUNT         3522 a         2201 c         2483 g         3309 t
ORIGIN      
        1 tttctgtaaa cacaagtcta gaagtgtgct ataatcatct catgaaaaaa ataagtgatt
       61 taggattaac aggtcgaaaa ttagttggag aaggtctaat cttagtcttc atcgggattg
      121 gatttttaat tgctggttgg caatttccag gactgattct tcgatttgtt cacgcaggtc
      181 tattcttttt agctttatat gaattgagca tgacattttt cagaaagaag aaatcgagtg
      241 aaagtgtcat tgcgctagtt gggaaggctg ttcttttcgg gatcctagcc agtctcgatt
      301 tagctgttca aattcccctc tatttcgcgg ctatttttgt tgggatttat caacttttta
      361 cagctcttat caatttcatt actttttacc tatatcgaaa agatggagta cagccacgga
      421 ttcgctattt aatagatgga atttggttat ccttactagg ttcagcttcc ctatttgtgt
      481 caggcacaca gctggttgtt caaaccattg tcatcggagg ttatctgatc ttgtatggac
      541 taactaatct gcgtgatggc ttcttgtttg aggaggcgat tgagcaacag agtctaaagc
      601 gccatgtacg cctaaccctc cccttgttcc ttgcagccct tgtaccacgg atgaccctgc
      661 aaaaagtcaa tgattacttg gcggataatg agggacaaac ggctcaatct atctataacc
      721 gccacaaaga aattgcggag tttccagcat tagaagtctt tgtacacgtt ggagaagaag
      781 ggttcggagc agttggacat gttgatttga gttacaaagg gcaggtctac ggttttggtt
      841 catatgatgt cctgtcagag cgtttgggtg gtgcgattgg tgacggagtt ttgtttagag
      901 ctgagcgtca ggcctatatt gatttttgta accaagaagg aatgactatg ttgggctatc
      961 aattggcatt gagttctgag caggaaaaag cggtagaaac tcgactagct gaaatagaag
     1021 gtttactgct tccatggcaa ccaagtgcgg aaaaggtctc tagacggtca gacggccagc
     1081 ctattgaaat gtatgcctac cgcatgaaag aggcaattgg ggcagtactt ttcaaattta
     1141 aaaaatcaaa attcaaaacc tactttgtcc tctcaaccaa ctgcgtcctt ttggctgatt
     1201 cagtaatcgg tcaagcagga acagatgtcc taggcttgcg cggtttcatt gctccaggaa
     1261 cctaccaatc ttatctggat caagaatacg aaaaacctca tagtttagta gtcgcaaaga
     1321 acatttatta tcgaaaagag aaatcctagt cactgactgg gattttctgt tattccagct
     1381 ctctaagttc cacaaaactc ccccatccat gctataatag agacatgaaa aaaaataaat
     1441 tattattaat tgatggctct tcggtagcct tccgtgcttt ctttgcactt tataatcaaa
     1501 ttgatcgttt taagagtccg acaggtcttc acaccaatgc tatctacggc tttaacctca
     1561 tgctggacca catgatgaag cggattgaac cgacccacat tctcgtggct tttgatgctg
     1621 gaaagaccac cttccgtacg gagatgtacg ccgattataa ggcaggtcgt gccaagacac
     1681 cagacgagtt ccgtgaacaa ttccccttca tccgtcagat gctggatgct atgggggtca
     1741 agcattatga gctggctcag tacgaggcag acgatattat cggtaccttg gacaaaatgg
     1801 cagagcggac agagattcct ttcgatgtga ccattgttag cggtgataag gacttgatcc
     1861 agctgacaga cgaaaacacc gtagttgaga tttctaagaa aggcgtagcc gaattcgaag
     1921 aatttactcc agcctacctc atggaaaaaa tggggattac ccctactcag tttattgacc
     1981 tcaaggcttt gatgggagat aagtccgata acattcctgg cgtgaccaaa atcggtgaaa
     2041 aaacaggtct aaaactcctg acagaatttg gttctttgga tggtatctac gaaaacattg
     2101 acagcatgaa ggcttctaag atgaaagaaa acctgattgc tgataaggag aaggccttcc
     2161 tatctcgtac acttgctacc attgatactc aggcaccaat cgaaattggt ctggatgaca
     2221 ttgtttatca aggccccaag gtggatgaac taggacaatt ttatgatgac atgggcttca
     2281 aacaattaag ggctcagtta ggtacgacca gctcgcaaga ggaagcggta ctcgatttcc
     2341 aaatcgtaac ggaaatcagc cccgctatgc tcaaacaaga ccagtttttc tattttgaaa
     2401 tcttgggtga aaattatcac cgagaggatt tggttggttt agcttgggga gataaggaga
     2461 aaatctatgt cggagggcct gagctacttg atagtccagt cttgcgtgac tttttagaaa
     2521 accaaactat aaaaacctat gactttaagc gtggaaaagt tcttctcgac cgaaaagaga
     2581 ttacgctacc tccagctact ttcgatagcc gtctagccaa atacttgctt tccaccgttg
     2641 aggacaattc cctaacaacc attgccaacc tctatggtca gatcagtctt gttccagatg
     2701 aggcagttta tggcaagggt gctaagttag acttgccaga acgcgagatc tttttcccac
     2761 acttggcacg taaggttcag gttttgattg agacagaaga gccgatgttg accaaattag
     2821 aagaaaatca gcaattagat cttttgtttg acatggagtt gccactggca aatgttttag
     2881 ctaagatgga aattgctggt attaaggtgg aagctgagac tctcaaagct atgcaatctg
     2941 aaaacgaagt tttgattgac cagttgacca aggaaatcta tgaactagct ggtcaggaat
     3001 ttaatatcaa ttcgcctaag cagcttggga ccattctctt tgaagagatg ggactaccgt
     3061 tggaatatac caagaaaacc aagacaggct attcaacagc agtagatgtc ttagaacgat
     3121 tagcgccaat tgcaccagtt gtttcaaaaa tcttggaata ccgtcagatt accaaacttc
     3181 aatcaactta tgtagtcggc ttgcaggatg ccattttaga agatggtaag atccataccc
     3241 gctatgtgca ggatttgaca caaacaggtc gtctatcttc gacggaccct aacttgcaaa
     3301 atattcctgt tcgtctagaa caagggcgct tgattcgtaa ggcatttgtg ccatcgcttg
     3361 aaaatagtgt ccttttggca tcggactatt cacagattga attgcgcgtc ttggctcata
     3421 tttctcagga caagcatttg attgaggcct tccagcaagg tgcagatatt cacacttcga
     3481 cagctatgcg ggtcttcggt atcgaaaaag ctgaggatgt gacaccaaat gaccgtcgga
     3541 atgccaaggc tgtaaacttc ggtgtcgtct atggtatttc agatttcggt ctatccaata
     3601 acttggggat tacacgtaag gaagctaaag cctatatcga tacctatttt gaacgcttcc
     3661 caggaattaa gaattacatg gaaaccattg ttcgtgaggc acgtgataag ggttatgtag
     3721 agacgattta caaacgccgt cgtgaattac cagatataaa ctctcgcaat ttcaatgtcc
     3781 gtaattttgc ggagcgaaca gctatcaact caccaattca aggttcggcc gcagatattc
     3841 tcaaagtagc catgattaat cttgacaagg ctttgactga agcaggtctt gcgacacgta
     3901 tgttattgca agtacacgat gagattgttt tggaagtccc agtggcagag ctagaaacgg
     3961 taaaagccat ggtaaaagag accatggaat cggcgattag cctgtctgtg ccattgattg
     4021 cggatgaaaa tgaaggttca acatggtatg aagcaaagta agaaaggaaa aagtatgaaa
     4081 aaattaattc tttgtattgg tctatgtagt cttctacttg caggttgcgg aaaagcatcg
     4141 cagaagactg agaaaacacc agcttcttca ggggaagagt tttcaaccac cttaccgatt
     4201 ttgcaagaaa aacaggatac tacgaatgaa cagttcaatg ttttagcaaa tgcagaggtt
     4261 gtagctgtta atagtacacc acctgccgac gaaacacaaa aaggccacaa gatttatgcg
     4321 gccaataatg gtgttgctga aaaagatgaa aatgggaata tcaaggaata tttccgttat
     4381 tataatgtgc catctacttg gacaattaat gcagaaaata ctaaggatga gacggtcgct
     4441 gcagtctacg atgttaaaga ggggggaagc aattacatgg ttcaactcta taatatcaat
     4501 gcttttaatc agtctccatt ggaagacggt cgcaatatga cgccggagga attagaagca
     4561 cggatggttg aaacaaatca ttctttcaat gaaaagacgg ttgtcacaat caatggtcag
     4621 gaatggcggg ttgggcgaca gttattaaca gatcaaaaat tagctcgtct gactttttat
     4681 cggatggaat cgacagctgc ttatgacgac tctgtggttg tgggttcaat ctattattcc
     4741 ttagatccag gaaccgataa agatcggacc aatcttaaaa aggttatcgg gcaagttaag
     4801 gatgtcgtgt accaaattgc taaaaaataa tcaaggtaga caagctataa tcagtaacga
     4861 gttaacactg acaagattgt gttatagcac accttattca ttaagctttt attgattcta
     4921 acatttaata tacattaaag gaggtctcta tgtcttattc cttccaaaat cctagtcagg
     4981 acattatctt tgactatctg aaaaatgcta agaccattgc agtggtcggt ttgtctagtc
     5041 gagaagagac ggcagcctac cgcgtttcca agctcatgca ggaagcgggc tataaaatta
     5101 ttccagttaa tcccaaggct gctggtggga ctattttagg cgagctggtc tatagtagtc
     5161 tagcagagat tgaccaacct attgatattg tagatgtttt ccgcagaagt gaatttttac
     5221 cagaggtagc acaggaattt atccagtcga acgccaaaat tttctgggct caactcggtt
     5281 tagaaagcca agaagcagaa aaactattgc gtcaggcagg tcgtaacgat attgtcatga
     5341 ataaatgtat caagattgaa tatcttgaaa tgaaagaaca atactaataa gtcgggtata
     5401 tttatccgac ttttttcatc gaaaaagcag ttcaatgaac cattgaactg ccggatgact
     5461 ctaaggagga gcacttagaa ttggaacaaa ttactacaca tggcgtaaaa aatagaaagg
     5521 aagaaatctg ctccaatacc aagcaggcta aaatagtcct ttgtcgctcc tcaattatag
     5581 tataacacaa tccaaatgtt tgtaccaatt attactatat ttttttctta aatatcaaca
     5641 caaaagtgta tataaaaata agagcaaata tcgctcttat tttatacgta actcattttc
     5701 ttagcctgat catctaaaat ttcttcatgg aagtgcattt gtgcagcata gtgatacggc
     5761 catacatata aacctgccaa tccaaatgta ataatgacaa gaaggaacca accaatgaaa
     5821 gataaatcaa gtgtaaagcg tttgaatttg taacctttca tcaactttcg gctagaccga
     5881 atgattgaaa aggcgtctgt atattcctcg cgttccaatt gttcaaacaa gatatattct
     5941 acctgtgagt aagcatagat ttgtggaata taaagagcag ttcctaaaac cataagtagg
     6001 atacctccca tggccattag ccctagtatg gcaaggattt ccataggaag tgcatcaggg
     6061 tctaaggatt gactggatgc agctataatc gtaatcatcg ttccagcacc gataagtaga
     6121 ccgataccaa ggtaaaacag tagtccccaa aggaagagca agaaactttt taaaatgtaa
     6181 gttcgaaaga ttttccagaa atctttatga ttgaaaatgc tcaaggaatc tttgaatgat
     6241 gtggtagttt tgcccccttt gatgatattg aagatagtcc atatgactga taatgacaac
     6301 attcctagta gcaatccgta aaatactgga aacaaagagg tagaaataaa gtagctcgga
     6361 ctagcaagtt cctcagccga cactccagca aggctattgc gagagaaaga aattaactga
     6421 acgataacag aaatagtgac ggggatgacg gccacttgat agataccagg tgtctcgttt
     6481 agcgtttggc gagcccgctc acgtatatta gaaattgtaa acatctacat aacctcctta
     6541 tcttaacatt gtaacataat tctagatgac aagctaggct ttttttgata gactggaagg
     6601 tgctgtggta tagtcgttta gtttgctttt atgaagtacg gcagagcgag ttcaaatact
     6661 ctagtggagt gtttgaagtt ggaaataagg aaacgaagtt tcctcgctcg tcgaagcaat
     6721 caaagataaa ctaaatgact ataatttgtc agtttgttgt gatgcgccta tctatttact
     6781 attaaataag tagaagacaa ggtaaatatc gctgaaataa gcgagaacat taaagacatt
     6841 ctgatggatt agaccatgca agattgagaa actgtatttc caggtaaagt acttttatgg
     6901 atgatatgaa agagctgaac tgtgaatcca gaactaatat cctaggactg tttttcctag
     6961 cgggaggtaa ataaaacatg acagatgttc caatcaagta tcgcttgatt aagaaagaaa
     7021 agcacacagg tgcacgtttg ggcgaaatca ttaccccaca cgggaccttt ccaactccga
     7081 tgtttatgcc agttggaact caggcaacgg taaaaaccat gtcgcccgaa gaattgaagg
     7141 cgatgggttc aggaattatc ctatctaata cctatcattt gtggcttcgt ccaggtgacg
     7201 aattggtggc gcgtgcaggc ggtttgcaca aatttatgaa ttgggatcaa ccgattctga
     7261 ctgactcagg tggtttccag gtttattctt tggcggatag tcgcaatatc tcagaagaag
     7321 gtgtgacctt caaaaaccat ctcaatggta gtaagatgtt cctgtcacct gaaaaagcta
     7381 tctctattca gaacaatctt ggctctgaca ttatgatgag ctttgatgag tgtccgcagt
     7441 tctaccagcc ttatgactat gtaaaaaaat ccatcgaaag aacaagtcgt tgggcagaac
     7501 gtggtctcaa ggctcaccgc cgtccgcatg accaaggttt atttggtatt gtccaaggtg
     7561 ctggttttga agatcttcgt cgccaatcag cccatgattt ggtcagcatg gattttccag
     7621 gatactctat tggaggattg gctgttggtg aaacacacga tgaaatgaat gcagtactgg
     7681 acttcacgac ccctctttta ccggagaata aaccacgcta tctcatgggc gttggagcac
     7741 cagatagctt gattgatggc gtaattcgtg gggttgatat gtttgactgt gtattaccaa
     7801 cacgcattgc gcgtaatgga acctgtatga ccagtcgtgg acgtcttgtg gtgaaaaatg
     7861 ctcagttcgc tgaggacttt acaccgttag atccagagtg cgattgctac acatgtaaaa
     7921 actatacacg tgcgtatctc cgccatttac ttaaggccga cgagacattt ggtattcgtt
     7981 tgacaagtta tcacaacctc tacttcttga ttaatttgat gaaaaatgtc cgtcaagcaa
     8041 tcatggatga caaccttttg gaattccgtc aagactttat ggaaaaatac ggttacggta
     8101 aaaacggacg taatttttag ataaaacagc cttctttgtg gacttagcct tgttctcata
     8161 ctcaatgaaa atcaaaatca aactaggcga cgcagatgca gatagaactg aagttcatca
     8221 aatcaagtca acaacgtctg attttgattt tcgaagagta tcagttacac cttctcaaga
     8281 tttggttaga tacaaataaa taaagcatac gattatgaaa cccctcggta acataatcgt
     8341 atgcttttat tttaatactt ggcttcacag cacttctatt ttaatgttct ttgaagatta
     8401 aaatgaacag ttgtcaacga gctttgatga gtgaaaaaac gattggtccc cattaccctt
     8461 caaaatacag taattctttc ggagtctttg tgaatacttc aaagccattt ttggtaacgt
     8521 aaccacaatc ctcgatacga acaccaactt ttccagggat gtaaatacct ggttcgacag
     8581 agaaacacat accttcttcg atgactaaat cattgccttc cataattgat gggaattcat
     8641 ggacactcat tcccaaacca tgaccgagtc ggtggttgaa gtactcgcca taacctgctt
     8701 tttcaatcac agagcgagcg gcatagtcaa tctcaccagc agtcacacca ggcttaatca
     8761 tattgaccgc ggccatgtga gcctccagag tcaagttgta aatatctttt ttaaactgat
     8821 caggtttacc gacagcgaca gtccgtgtca tatcagacgt atagcccaaa gcttccacgc
     8881 ctaggtcgaa caatagcaga gcgttattct cgattttatt tgttgaggga ataccgtgtg
     8941 gatttgcggc attgtcgcct gttaagacca tggtgtcaaa gctcattttt gagatgcctt
     9001 gtttcttcat ttcaaattca atctgagcaa taatatcagt ttcagttacg ttgagggaga
     9061 tattgttaaa accaacctgc atagccttat ccgcaaactc tccagcaacc agcatttttt
     9121 caatttcatc acgcgacttg atcaatttca tagtattaat cagaggtgta atgtctgaaa
     9181 atggttggct aaagatagac tgaagaccat ggtagcgggt taaattcaaa ttatcaaatt
     9241 ctgcacagat tactgagaaa gatttctgtg gcaatttgct cttgataatc tgccatggat
     9301 tttctgagtc catatagccg gctacaggaa agtcaacagt tgcaactgca cgttctacat
     9361 ccaaggcagg gagaaagagt agtgagtcgt ggtcaggcat aacaaagagc atcatgtggc
     9421 gctcatgagg atcgctatga tagccagtca gataatagat gctaactgga tcagagaaga
     9481 tggccaagtc cattttgttg gcttctaagt aggatttaac atgatgtaat tttgacatag
     9541 tggagacctt tctagcatgt agtggtaaaa aaagtgaaat tttcgtttat tatatagact
     9601 attgtttcaa aaaatgaaaa aaattgcaag tattatcaaa aaaatagagt ttatctcttg
     9661 aaagtggttt caaaaagtga tacactatac ttgagtgaaa gcgtaatttt catagaacta
     9721 gaactattga aagggagtac ataacgtatg ttaaacactg acgatacggt aacgatttat
     9781 gacgttgccc gcgaagcagg tgtatccatg gcgacagtat cgcgcgtggt aaatgggaat
     9841 aaaaacgtaa aggaaaatac tcgtaaaaaa gtattagaag tcatcgaccg tttggattat
     9901 cgtccgaatg ctgttgcacg tggcttggcc agcaagaaga ctaccactgt gggggttgtg
     9961 attccaaata ttgctaatgc ttattttgca accttggcca aaggtatcga tgatattgcc
    10021 gatatgtaca aatacaatat cgtcttagca aacagtgatg aaaatgatga gaaagaaatc
    10081 aatgtggtga ataccctatt ctcaaaacag gtggacggaa tcattttcat gggctatcat
    10141 ttgacagaca agattcgtgc ggagttttca cgctcacgta caccgattgt tttagctggt
    10201 accgtggact tggagcacca attacctagc gtcaatattg actacgctgc cgctagtgtt
    10261 gatgcagtca atctattagc taagaacaac aagaaaattg cctttgtatc agggccgctt
    10321 gtagatgaca tcaacggtaa agttcgtttt gcaggctaca aacaaggctt gaaggacaac
    10381 ggaatcgagt ttaacgaagg attggttttt gaatccaagt ataaatacga ggaaggctac
    10441 gctctagcag aacgtatttt gaatgccgga gcaactgcag cttatgttgc agaagatgag
    10501 attgctgctg gtttattgaa tggtgtcagt gatatgggcg tcaaggttcc agaagacttt
    10561 gaaatcatta caagtgatga ttccctagtg actaagttta cccgtccaaa cctgacctct
    10621 atcaatcagc cactatatga tattggtgca attgctatgc gcatgctcac caaaatcatg
    10681 cataaggaag agttggaaaa ccgtgaggta gttcttaacc acggaatcaa agtacggaaa
    10741 tcaactaagt gataaatatg ggagtagccg gataagatat cttattactc cgcaccctca
    10801 ctctatcaaa cattataatc atttggttta tcttttatta cttcgacgag cgaggaaata
    10861 tttccaacct agaacagcct ttaggctgtt ctagcaactt gtagctattg aggcaaactt
    10921 gcttcgactt atctccagcc ttcaaaggtt cggagaacct ttgaagcgag tactaaaagc
    10981 aaacaaaatg gctatactaa ccgaaaacta aaagattata aaaaataaat ataaagccct
    11041 cgctagatgt ataagcttag cgagggcttt gtggttgatt ttaagtctgt cacactctgt
    11101 aagttgtgcg agatgttgat gcgaatctag agaagcagta ctgggcttgt tctcatcctt
    11161 tagaactgta tcagttaggt gaattataat ttacgtttac gtgtaaacaa cgcaattgca
    11221 agcccgataa taccacttgt caataaccat aaacttcctt tctcaccagt tactggtaag
    11281 atttgcttag cagtttcggt tttaaatgtt gaagtctcct gcttgtctgt tttttcagtt
    11341 gttggtttag tgtttgaatt ttctccgact gtaccatttt ggttgttccc attaacaggt
    11401 atctgttttt tagaggtttc gatattttcc ttctctttat tagctatttg cctttcaggt
    11461 gttacggcat tgtccttctc attaacaggc atctgttttt cagaggtttc aacat
//
LOCUS       DASFSW010000065        11442 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.065, whole genome shotgun sequence.
ACCESSION   DASFSW010000065 DASFSW010000000
VERSION     DASFSW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.065"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..404
                     /locus_tag="U1099_001237"
     CDS             <1..404
                     /locus_tag="U1099_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867557.1"
                     /translation="RLELEQHRLNYMADDTPIEPSDIPKLMEIAKKLQAEDTSLNIYE
                     LYKHPEARAKLFSQITEACYMALNATPTQAQRLAFCDYLEQQYENTLKKMVASTDKQA
                     LGELLDLLELPVEIESQFIRDMAISGLLAKG"
     gene            474..974
                     /locus_tag="U1099_001238"
     CDS             474..974
                     /locus_tag="U1099_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867558.1"
                     /translation="MIELTIESITKPLKQKRLSRVSGKLKGVGITIDLNNLTVNYGSQ
                     QINLASIPGTYGGVRYFFLCPICEKRCRKLFKNSLIFACGTCQDRHRATLNRSKTDCQ
                     YYFELAFKEARKIDPSYYPEKGYVDYDNFPSRPKRMRAKTYWKHYRKFTSYLDKGVSN
                     WLGGLK"
     gene            1114..1500
                     /locus_tag="U1099_001239"
     CDS             1114..1500
                     /locus_tag="U1099_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4867559.1"
                     /translation="MFEINMTINERLRDIRDLKDAISSLENDKLELEKTYPVQSRRIR
                     KKKARLLVAIRGIKVKRQRMTDLINQLSDENQRKILTLQYIEGVKDKHLVEVSGLKDY
                     REVSSIRQKAIKNLERLQKQLEQPQA"
     gene            1684..1866
                     /locus_tag="U1099_001240"
     CDS             1684..1866
                     /locus_tag="U1099_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002961998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEM4867560.1"
                     /translation="MPMTQKEMVKLLTAHGWTKTKGGKGSHVKLEKVGERPITIPHGE
                     INKYTERGIKKQAGLL"
     gene            1906..2358
                     /locus_tag="U1099_001241"
     CDS             1906..2358
                     /locus_tag="U1099_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEM4867561.1"
                     /translation="MLVTYPALFYYDDTDGATAPYFVTFPDFEHSATQGEDMADAMAM
                     ASDWLGIHLADYIENGRDIPTPTPINALSLADNNPFRDDKDIELVYDPSKSFVSMVMV
                     DVAEYLGSQEPVKKTLTIPRWADTLGRELGLNFSQTLTDAIADKKIHA"
     gene            2470..2679
                     /locus_tag="U1099_001242"
     CDS             2470..2679
                     /locus_tag="U1099_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867562.1"
                     /translation="MAKPKEKWTPIITHIVPKDTELADGTILDKETTLSQEEFSKNPP
                     VIPDGHPFYNIWARIIREKIEKGEL"
     gene            3786..4007
                     /locus_tag="U1099_001243"
     CDS             3786..4007
                     /locus_tag="U1099_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation repressor RelB"
                     /protein_id="HEM4867563.1"
                     /translation="MSTVTIRLNQEEEVFFKSYAQLTGQSLSSLFKKALERDIEDEYD
                     LKIYHQAYDEYKADPETISHADFKKELGL"
     gene            4009..4269
                     /locus_tag="U1099_001244"
     CDS             4009..4269
                     /locus_tag="U1099_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM4867564.1"
                     /translation="MYHLEYSKKAQKQIKKLDRQIQRLLFAWIDKHLEGTDNPRANGK
                     GLTGNHANEWRYRIGDYRLICDIQDDKLVILALEFGHRKDVY"
     gene            4436..5077
                     /locus_tag="U1099_001245"
     CDS             4436..5077
                     /locus_tag="U1099_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4867565.1"
                     /translation="MIEWIFFDLGSTLLDEEAAYGYYIDKCVKKLESLDIEVSSDSYK
                     KKMVEYAHKSLDPIRATWHYFAPTEPRPLWTNEGVSLYPETIDALEKLSQNYRLGIIA
                     NQSSSIRELLKEWGIESYFQLIILSEEVGLSKPNTAIFTLALQKTNIPADRVVYVGDR
                     YDNDILPAKSLGMWTVRILTGFGKHASENEKLKSDWVIPSLQEITNIFEQTKN"
     gene            complement(5110..5295)
                     /locus_tag="U1099_001246"
     CDS             complement(5110..5295)
                     /locus_tag="U1099_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867566.1"
                     /translation="MNPVDLFNQVKELIANKDFDGAKQFIEENKDQFGEYLEQAKGLL
                     SGSEGVNGLLDKVKGLF"
     gene            complement(5359..6363)
                     /locus_tag="U1099_001247"
     CDS             complement(5359..6363)
                     /locus_tag="U1099_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEM4867567.1"
                     /translation="MAIYFGTYTKRESKGIYKANFDSETGQLSNLELVTEEPNPTYLA
                     FDKAGHLYSVGAENGLGGIAAFNAEYTLLNHVVSEGAPLCYVAVDEARDLVYGSNFHK
                     GQLLVYKRLADGSLELADVAQHEGSGPHENQASAHVHFSDLTPDKFLVTCDLGCDQVV
                     TYKVSDQGKVEKIATYQAAPGSGPRHIVFHPVAKIAYLICELNSTIEVLIYDGWGQFE
                     HLQTVSTLPEDHTGFNGTAAIRITKDGKFIYGSNRGNDSIAVYKTLADASLELVEIVP
                     TNGKTPRDFTLSPDEKHLIVVHQDSDNATVFERDAENGCLTELSHDFYVSEAVCVTFL
                     "
     gene            6499..7665
                     /locus_tag="U1099_001248"
     CDS             6499..7665
                     /locus_tag="U1099_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM4867568.1"
                     /translation="MEDKHQKFSLTWFFRLFLNSQAVTFLLVTLLTFLTIFIFSKISF
                     LFRPIGSFLEIVLLPMILTGLLYYLLNPMVDWMEKHKISRTVGISILFVLISLLIIWG
                     LAVAIPSIQEQVTSFAQNLPSNIQKIEGQVTGLLQDQRFEQFRPTALEMLNKVNDQIV
                     AYAQKFSSSAVNWASNLISTASQIIVAILIMPFILFYLLRDGQYLNKHITQYLPTKWR
                     EPIGTVLSDVNGQLSNYVRGQVTVAIIVALMFSVMFSIIGLSYPITLGVMAGFLNLIP
                     YLGSFLAMIPAVILGLIAGPIMLIKVLVVFMIEQTIEGRFVTPLIIGSSLSIHPITIL
                     FVLLTAGQMYGVLGVLLGIPIYASIKVLVKAAFEWYKAHSSLYEDEENEVVNEQ"
     gene            7655..8884
                     /locus_tag="U1099_001249"
     CDS             7655..8884
                     /locus_tag="U1099_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEM4867569.1"
                     /translation="MNNSEKMLVCLDQQDLDKADKYFKRALVQDDSETLLSLAAYLEG
                     IGFFPQARQIYEQVKEEFPEVLINLAQIAFEDGLVEEAFRYLEEISEDSSIYVEALLV
                     KADLYQAEGLSDVACEKLLEASYLSDEPIILFGLAELDMELELFNEAISYYAQLDNRE
                     IYELMGVSTYQRIGIAYASLGKFEAAIEFLEKAVELEYDDQTVFELAALLFEREEYQK
                     ANLYFKQLDTINPDFEGYEYAYAQSLHAEHKIDDALVMTQKGLAKNDFDANLLLQASQ
                     YAYELHDEAGAEDYLLRAKEVADDSNELALRLSNLYLEQERFEEVVALFDEEVDNVLA
                     RWNKAKAYQALERDDEAIALYDELAGELAENPEFLADYVAVLRQLGRLDEAKEQASRY
                     IQLVPDDLAMQEFLNEE"
     gene            8888..9604
                     /gene="budA"
                     /locus_tag="U1099_001250"
     CDS             8888..9604
                     /gene="budA"
                     /locus_tag="U1099_001250"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEM4867570.1"
                     /translation="MQVNRLFQYNTLGALMAGLYGGSLTVGELLEHGDLGLGTLDSID
                     GELIVLDGKAYQAKGAGEKPEVVEVPADMKVPYAAVIFHEAEVIFKQRFEMTSDELHQ
                     RIESYYDGENLFRSIKIKGTFSHMHVRMIPKSAFDVRFAEVASRQPEYTAENISGTIV
                     GIWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDYVISEGMVEVGPVDQLDQRFPVQDR
                     QYLFAKFNAKEVREDIDKAE"
     gene            complement(9630..11288)
                     /locus_tag="U1099_001251"
     CDS             complement(9630..11288)
                     /locus_tag="U1099_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_147463931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEM4867571.1"
                     /translation="MSFDGFFLHHMTAELKANLEGGRIQKINQPFEQEIILNIRSNRQ
                     SHKLLLSAHSVFGRVQLTQSDFTNPKVPNTFTMILRKYLQGAIIEEIRQLDNDRILEF
                     SVSNKDEIGDHTQATLIVEIMGKHSNIILVDKSEQKIIEAIKHVGFSQNSYRTILPGS
                     TYIRPPETHSLNPYTVSDEKLFEILSTQELSPKNLQQVFQGLGRDTASELASHLQTDR
                     LKNFRAFFDQATQPSLTAKSYAALPFANSPENQPHFESLSSLLDFYYQDKAERDRVAQ
                     QANELIKRVASELEKNRKKLVKQEQELADTETAELVRQKGELLTTYLHQVPNDQASVT
                     LDNYYTGQELEIELDVALTPSQNAQRYFKKYQKLKEAVKHLTNLIEETKATIVYLESV
                     DTMLGQASLAEIDEIREELIETGYLKRRHREKIHKRQKPERYLATDGKTIILVGKNNL
                     QNDELTFKMAKKGELWFHTKDIPGSHVVITDNLDPSDEVKTDAAELAAYFSKARHSNL
                     VQVDMIEAKKLHKPTGGKPGFVTYRGQKTLRVTPTEDKIKSMKI"
BASE COUNT         3449 a         2056 c         2499 g         3438 t
ORIGIN      
        1 acagattaga acttgagcaa caccgcctaa attatatggc tgatgataca cctattgagc
       61 ctagcgatat tcctaaacta atggaaatag ctaaaaagtt acaagcagag gataccagtc
      121 tgaacattta tgaactgtat aagcacccag aagcgcgtgc taaactattc tcacagataa
      181 ctgaagcttg ttatatggct ttgaatgcta caccgacaca agcccaaaga ttggcatttt
      241 gcgactatct agaacagcaa tacgaaaaca ccttaaagaa aatggttgcc agcacagaca
      301 aacaggcact aggtgagtta ctagatttgt tagagctacc agtagagata gaaagccagt
      361 ttatccgaga tatggcaatc agtgggttac tagccaaagg ctgaacaggg aggcacgcgc
      421 ctcttttgtt gtttaatcaa taaatttggg ttttgccaat aagggagggg aaaatgattg
      481 aattgaccat agaaagcata accaaaccat tgaaacaaaa acgcttatca agagtatccg
      541 gtaagttaaa gggagtaggt atcactattg accttaataa cctaactgtt aactacggta
      601 gccaacagat aaacctagct agcataccag gcacttatgg aggtgtccgc tacttctttc
      661 tttgtcctat ttgtgaaaag aggtgtagaa aactattcaa aaatagcctt atctttgctt
      721 gcggtacttg tcaagatagg catagggcaa ctttgaaccg aagtaaaaca gactgccagt
      781 attattttga actagctttc aaagaggcta ggaagataga ccccagttat tacccagaaa
      841 aagggtacgt tgattatgat aattttccta gcagaccaaa aagaatgagg gctaagacat
      901 actggaaaca ttacagaaag tttactagct atttagataa aggggtgagc aattggttgg
      961 gtggactaaa ataacagggt gcatttacaa aacaaacaaa taaagaacgc tctattagtt
     1021 cccaaaacaa ataaacttac tcaatcgaac aaagaacgaa caaagaatac gcgctttttt
     1081 gatataatca tagtcatagc gaggtattaa attatgtttg aaatcaatat gacgattaac
     1141 gagcgactaa gagacattag agatttgaaa gatgctattt ctagtttaga aaatgataaa
     1201 cttgaacttg agaaaactta tccagtacag tctaggagaa ttagaaagaa aaaagcaaga
     1261 ttattggtag caatacgagg aattaaagta aaacggcaac gtatgacaga tttaatcaat
     1321 cagctatcag acgaaaacca acgtaaaatt ttgaccttac aatatatcga aggagtaaaa
     1381 gacaaacatc tagtagaggt aagcggtcta aaagattata gagaagtctc tagcattaga
     1441 caaaaagcta ttaagaactt agagcggtta caaaagcaac tagaacagcc ccaagcttaa
     1501 atttagccct attttagcgc gtgttttgct ttatccgata aattaaccat acagcaacta
     1561 aaagccctta gaaacgattt taggggctat ttttgataac tgatattaga caaaatatta
     1621 tctttttact tgaatacgtg tagtacgtgt gatataatga tgttgtcagg aggtaaaagc
     1681 gctatgccta tgacacaaaa agaaatggtt aagctactta ccgcccatgg ttggacaaaa
     1741 actaaaggtg gtaaggggtc tcacgttaaa cttgaaaaag taggcgagcg acctataacc
     1801 attccccacg gtgaaattaa caaatacacc gaaaggggta tcaaaaagca agcagggctg
     1861 ttataaaagc agtccctcgc ttgtctttga ttttggaggt aaactatgct tgtcacttat
     1921 ccagctttat tttattatga cgatacagac ggagctaccg ccccttattt tgtcactttc
     1981 ccagactttg aacattcagc aactcagggc gaagatatgg cagacgctat ggcaatggct
     2041 agtgattggt taggtattca tctagctgat tatattgaga atgggcgtga tattcccact
     2101 cctaccccta ttaacgcttt atcattggct gataataacc ctttccgtga tgataaggac
     2161 atagagctag tttatgaccc tagcaagtct tttgtatcta tggttatggt tgacgttgct
     2221 gagtatctag gcagtcaaga gcctgtaaag aaaacactaa ctattccacg ttgggcggat
     2281 actttagggc gtgagttagg gctaaatttc tcacagacct taacagacgc tattgcagat
     2341 aagaaaattc atgcttaaca ctatctaact tatatgatag ggctaaacca gtacactaaa
     2401 acaggtaact gctaaggtgt aaggttagcc ctattttgta tttttacaca ataaaggagt
     2461 aattttaaca tggctaaacc taaagaaaaa tggacaccaa taataaccca cattgtccct
     2521 aaagatactg aactagcaga cggcacaata ttagacaaag aaaccacctt atcacaagaa
     2581 gaattttcta aaaatccccc tgttattcca gacggacatc ctttttataa tatttgggct
     2641 aggataatta gagagaagat agaaaagggt gagctatagc accaagatac agtagaaaac
     2701 agtaaaatac agtagatttg cgcgtgataa ttgcttgaaa ttgatttgtc aaggtcaaca
     2761 aaattgataa ccacgcgcat ttaagctact taatttacgg ttgataaaat cccaatacgg
     2821 taaagtttta accttttcca atttggaaac agttatctat cttttaaaaa tggcatattg
     2881 tgagaaataa ctaacttgtg ccagctatcc gtggtataat atattcacag ctggaggctt
     2941 gcatagtcta gccgattagg gagagttaac ccgtgaggtc atacactccc ttttttggta
     3001 taaaatagct atcagcaaga catcagataa ctaaaaagca ccttatcaag tgtacttaag
     3061 atttgctttt gtaaagtgaa tatgatagca tgaaaatata gtatccttta caggttccac
     3121 cgctattctg atagcaaagg agcggaatat agtgcgacat tgaatgccgc tcagtgataa
     3181 acgtaccagc caatatgtcg ttagttttgc tggtgtcccc tgttggtggt tgaggggata
     3241 gtgaggaggg gtatccctct tttttgtatg gtaattaaga agacctgaaa aaagcacctt
     3301 gtgggggtgc tagttcttgc ctgctgaact catcgtttta ataccttttt tactacccct
     3361 ctagtatatt gtacttgttt gtactttaca ttttagaacg aaaaaggtaa aaagtagatt
     3421 agtaatttag cgtattttat tgaactattt gatattatga caacttgaaa aaataaaaca
     3481 gtctgggaga ctgttttatc cctgagctag catagcgagg gttatagtaa gaacaagtct
     3541 ggaaatttcc agacttgttt ttcgtagtac agtgtactaa tttatccccg agctagtata
     3601 gtgagggata tagtaagaac aagtctgaaa atattcggac ttgttctttg actttatatg
     3661 atttaagggc tgtaaagcct tgatataagg cactttgggg catcatccca aggtactttt
     3721 ttgatagatt gacagatact ttaaaaatgt gttacaatgt aacacaaaga agcgaggtga
     3781 atatcatgag tacagtgaca atcagattaa atcaagaaga agaagtattc tttaaaagct
     3841 atgcacaatt aacaggtcaa agtctttcta gcctttttaa gaaagctttg gagcgtgata
     3901 ttgaagatga gtatgattta aaaatctatc atcaagctta tgatgagtac aaagcagatc
     3961 cagaaacaat tagtcatgct gattttaaga aagagctagg attatagcat gtatcattta
     4021 gagtattcta aaaaggctca aaagcaaatt aagaaattag ataggcaaat tcagaggttg
     4081 ttgtttgctt ggattgataa acacttagaa gggactgata atccacgcgc caatggtaaa
     4141 ggtttgacag gcaatcatgc taacgagtgg cgttatcgta tcggagatta ccgactgatt
     4201 tgcgatattc aagatgataa gttggttatt ttagcacttg agtttggaca tcgtaaagat
     4261 gtctattgat gcgtgaaaat ctatgacaag ataggtccta actattttta agaatgaata
     4321 gaaaactaga ctgaaattcc tagggaattt tctttgcctt gatattgata aaatgatatt
     4381 ctaaaaaggc agatcaactt aagtattctc attttgagaa gagatggaga gtgggatgat
     4441 agagtggata ttttttgatt tgggttcgac gcttttagat gaagaggctg cttatggtta
     4501 ttatatagac aagtgtgtga agaagttaga gtctctggat atagaggttt catcagattc
     4561 ttataaaaag aaaatggtgg agtatgctca taagtcgcta gatccaattc gtgcgacctg
     4621 gcactatttt gcaccgaccg agccacgtcc tttatggaca aatgagggtg tgagcctgta
     4681 tcctgaaaca atagatgctc tagagaagtt atcacaaaac tatcgattgg ggattattgc
     4741 caatcagtct agtagtatta gagaactgct taaagagtgg ggaattgagt cttattttca
     4801 actcatcatc ctctctgaag aggttggttt atctaaacca aatacagcta ttttcacact
     4861 tgccttgcaa aaaacaaaca tccctgcaga tagagttgtc tatgtcgggg ataggtatga
     4921 taatgatatt ctgcctgcta aatctttggg aatgtggact gtacggatac tgacaggttt
     4981 tgggaagcat gccagtgaaa atgaaaaatt gaagagcgat tgggtaattc cttcattgca
     5041 agagataaca aatatttttg aacaaacaaa aaactagact gaatattctc agcctagttt
     5101 ttacatgtat tagaacaagc ctttaacttt atctaaaaga ccgttaacac cttcagaacc
     5161 tgataacaag ccttttgcct gttccaagta ttcaccgaat tggtccttgt tttcttcgat
     5221 aaattgtttt gcaccatcaa agtctttgtt tgcaatcaat tcttttactt ggttaaaaag
     5281 atctactgga ttcatgttga aatcacctcc tatgagacta ttaaaccaaa actgtacgga
     5341 tttcacaatt gaaacgactt ataaaaatgt aacgcaaact gcttcagaaa catagaaatc
     5401 gtgtgaaagc tcagtcaaac agccattttc ggcatctctt tcgaatacag ttgcattgtc
     5461 agaatcttga tggacaacaa tcaaatgctt ttcatcagga ctaagcgtga aatcgcgagg
     5521 agtttttcca tttgttggga cgatttcaac taattctaag ctggcatcag caagtgtttt
     5581 gtagactgcg atagagtcgt ttccgcggtt ggatccataa ataaatttac catcctttgt
     5641 gatgcgaata gcggcagtac cgttgaaacc agtatggtct tctgggagag tagagacagt
     5701 ttgtaggtgt tcaaattgtc cccagccgtc atagataagc acctcaatag ttgaattgag
     5761 ttcacagatg agataggcga tttttgcgac aggatggaag acaatgtgac gtggaccact
     5821 tcctggcgca gcctgatagg ttgctatttt ctctactttt ccttggtcag ataccttata
     5881 ggttaccact tgatcacatc ctaaatcaca ggttactaaa aatttatcgg gcgtcaaatc
     5941 agaaaagtga acgtgggcgg aagcttggtt ttcgtgcggc ccagatcctt cgtgttgagc
     6001 gacatcagct aattctagcg aaccatccgc caaacgttta taaacaagta gttgcccttt
     6061 gtggaaattg gaaccataaa ctaaatctct tgcttcatct acagcgacat agcaaagtgg
     6121 tgcgccctct gatacaacat ggttgagtag agtatactca gcattaaagg cagcgatacc
     6181 tcctagacca ttttcagcgc cgacggagta tagatgtccg gccttatcaa aggcaaggta
     6241 ggtagggtta ggttcttcag tgaccaattc tagattgcta agttgtcctg tttcagagtc
     6301 aaaatttgct ttataaattc cttttgattc acgttttgta taggtaccga aatagatagc
     6361 cataggattc tcctttttgg aaatgattga taaaattata ccatgttttt ctgagaaagt
     6421 cgtataaatt ctgtagttga tttttacaaa aatcctttct ccatgttaca atagaaaaaa
     6481 agactaggag tgtattagat ggaggataag catcaaaaat ttagtctgac ttggtttttc
     6541 cgcttgtttt taaatagcca agcggtgaca tttttattgg taaccttgct gacttttttg
     6601 accatattta tttttagtaa aattagtttt ttattcagac caataggaag ctttttagaa
     6661 attgtattgt taccgatgat tttaacaggt cttttgtatt acctattaaa tccgatggtt
     6721 gactggatgg aaaagcataa aatttctcgt acagttggta tttccattct ctttgtttta
     6781 atcagtcttc tcattatctg gggactggcg gtggcaattc cgagtattca agagcaagtg
     6841 acatcttttg cgcaaaatct tccttcaaat atccagaaaa tcgaaggaca ggtaactggg
     6901 ctcctacagg atcagcgctt tgaacaattt agaccaacag ctctggagat gttaaataag
     6961 gtcaatgatc agattgttgc ctatgctcag aagttttctt caagcgctgt gaactgggca
     7021 agtaatttga tttcaaccgc ttcacagatt attgttgcta ttttgattat gccctttatt
     7081 ctcttttatc tgttacggga tggtcagtat ttgaataaac acattaccca atatctacca
     7141 accaagtgga gagaaccgat tggaacagta ctatcagatg tgaatgggca attatcaaac
     7201 tatgttcgtg gtcaggtgac cgttgcgatt attgttgccc tgatgttctc agtgatgttc
     7261 tcaatcattg gtcttagcta tccaataact ttgggggtga tggctggttt cctcaatcta
     7321 attccgtatt tgggttcttt cttggccatg attccagctg ttattttagg attgattgct
     7381 ggtccaatca tgctgataaa ggttttggta gtctttatga ttgagcagac tatagaaggt
     7441 cgttttgtga caccattgat tatcggaagc tccctaagta ttcatccgat taccatttta
     7501 tttgtattat tgacagctgg tcagatgtac ggtgtcctag gagttcttct gggaattcca
     7561 atatatgcct ctattaaggt tttagtgaaa gcggcatttg aatggtacaa ggcacacagt
     7621 agtttgtatg aagatgaaga aaatgaggtt gtgaatgaac aatagtgaaa aaatgttggt
     7681 ttgtttggac caacaggatt tagacaaggc agataagtat ttcaagcgcg ccttggtgca
     7741 agatgatagc gaaacgcttt tgtccttggc agcctactta gaaggcattg gttttttccc
     7801 acaggctaga caaatctatg aacaggtcaa agaagaattt cccgaagtac tgatcaatct
     7861 tgcccaaata gcatttgaag atggcttggt tgaagaagct ttcagatatt tggaggaaat
     7921 ttcggaagac agttctatct atgtggaagc cttgttggtc aaggcggacc tgtatcaggc
     7981 agaaggcttg tcggatgtgg cgtgtgaaaa attgttagaa gcaagttact tatcagatga
     8041 acccatcatc ctctttggct tagcagaatt ggatatggaa ctggagcttt ttaatgaggc
     8101 tatttcctac tatgcccagc tggataatcg tgaaatctac gaattaatgg gagtttctac
     8161 ctatcagcga attggtattg cctatgcaag tttggggaaa tttgaagcgg cgattgaatt
     8221 tttggaaaaa gctgttgagt tggaatacga tgatcagacg gtctttgaat tggctgctct
     8281 gctatttgaa cgagaagaat accaaaaggc caatctctat ttcaagcagt tggataccat
     8341 caatcctgat tttgagggct atgaatatgc ctatgctcag tctcttcatg ccgagcataa
     8401 gattgatgat gccttggtta tgactcaaaa aggcctggct aaaaatgact ttgatgccaa
     8461 tctcttgctt caggcttcac agtatgccta tgaattgcac gacgaggcgg gggcagaaga
     8521 ttatcttcta cgggcaaaag aagtggcaga tgatagtaac gaactagctc tgcgcttgtc
     8581 caatttgtat cttgagcagg aacgatttga agaggttgtg gctttatttg atgaagaggt
     8641 agataacgtt ctagctcgct ggaataaggc caaagcctat caagctctag aaagagatga
     8701 tgaagctatt gcgctttacg atgaattggc tggagaattg gctgagaatc ctgaattttt
     8761 ggctgattac gtagcggttt tacgtcaact tggtcgtttg gatgaggcaa aagagcaagc
     8821 tagtcgttat atccaattgg taccagacga tttagccatg caagaatttt tgaatgaaga
     8881 gtaggttatg caagtaaatc gattatttca atacaatacc ttaggtgcct taatggctgg
     8941 actgtatggc ggctccttga cagttggcga attgttggaa catggtgatt tgggtttggg
     9001 aaccctggat tccattgatg gcgagctgat tgtcttggat ggaaaggctt atcaggccaa
     9061 gggtgcaggg gagaaaccag aagtggttga agttccagct gatatgaaag ttccctatgc
     9121 ggcggttatt ttccatgaag cagaagtcat tttcaaacaa cgctttgaaa tgactagcga
     9181 tgaattgcac cagcggattg agtcatacta tgacggggaa aatctttttc gctccatcaa
     9241 aatcaaaggg acgttttcgc atatgcacgt gcgcatgatt ccaaaatcgg cttttgatgt
     9301 tcgttttgcg gaggtggcaa gtcgccagcc tgagtatacc gctgagaata tttcaggtac
     9361 cattgttggt atttggacac cagaaatttt tcacggggtc agtgtggctg gctatcattt
     9421 gcattttatt tcggatgatt tgacatttgg cgggcatgtc atggactatg tgatttcaga
     9481 aggaatggtt gaagtgggac cagtcgatca actagaccaa cgttttcctg ttcaagaccg
     9541 ccagtatctt tttgctaagt tcaacgctaa ggaagttaga gaagatattg ataaggcgga
     9601 gtaggtttca atacagaaaa atccatgaat tagattttca tggattttat tttatcttca
     9661 gttggggtga cacggagggt tttctgacct cggtaggtca caaagcctgg tttgccacct
     9721 gttggcttat ggagtttttt ggcttcaatc atatcgactt ggaccaaatt ggaatgtctg
     9781 gccttagaga agtaggctgc taattcagct gcgtctgtct tgacctcgtc acttgggtcc
     9841 aagttgtctg tgatgaccac atgactacct ggaatgtctt tggtatggaa ccagagttcg
     9901 cctttcttgg ccattttgaa ggtcaactca tcattttgca ggttattttt cccgaccaga
     9961 ataatagtct tcccgtccgt cgccaagtat cgctcaggtt tctgacgctt atggattttc
    10021 tcacggtggc ggcgtttgag gtagcctgtt tcaatcaatt cctcacggat ttcatcgatt
    10081 tctgccagac tagcctgtcc cagcatggtg tcaacggact ccagatagac aatggttgcc
    10141 ttggtctcct caatcaagtt ggtcaggtgc ttgaccgcct ccttgagttt ctggtacttt
    10201 ttgaaataac gttgggcatt ctggctagga gtaagagcca cgtccaactc aatctccagt
    10261 tcctggcccg tatagtagtt gtctaaggtc acactagcct ggtcatttgg tacttgatgc
    10321 agataggtgg tcaagagctc gcccttttgt cgcaccaact ccgccgtctc cgtatccgcc
    10381 aattcctgct cctgcttgac cagtttcttg cgatttttct ccaactcact ggctacccgc
    10441 ttgatcagct cattggcctg ctgggccacc cgatcccgct ccgccttgtc ctgatagtag
    10501 aagtccagca gactgctcag gctctcaaag tgtggctggt tttcagggct attggcaaat
    10561 ggcagagcag cataggattt agctgtcagg ctaggctgag ttgcctggtc aaaaaaggca
    10621 cggaagttct tgaggcggtc cgtctgtaag tgactggcca gttcagaagc tgtatcccgc
    10681 cccaaacctt gaaagacctg ttggagattt tttggactca gttcctgagt cgataagatt
    10741 tcaaacaatt tctcatcaga caccgtataa ggattgagag aatgtgtctc cggtggacga
    10801 atataggtgg agcctggcaa gatagttcgg taggagtttt gcgagaaacc aacgtgcttg
    10861 atagcctcaa taatcttctg ttcagactta tctaccaaga tgatattgct gtgcttgccc
    10921 atgatttcca caatcaaggt tgcttgggta tggtcgccaa tctcgtcctt gttggacacc
    10981 gaaaattcta agatgcggtc attgtccaac tgacgaatct cctcgataat ggctccctgc
    11041 aagtatttcc gcagaatcat ggtaaaggta ttgggcactt tgggattggt aaagtccgac
    11101 tgggtcagct gaacccgacc gaaaaccgaa tgggcggaca agagcaattt atggctctga
    11161 cggttgctac ggatattgag gataatctcc tgttcaaagg gctggttgat tttctgaatc
    11221 cgcccacctt ctaaattggc ctttaactca gccgtcatgt gatgtaaaaa aaatccgtca
    11281 aaagacatgg ttttcctctt tctaatagct ataactcagt ctagtatatc ataaatcagt
    11341 gactttttaa cgaaggggac taaaagaagt attcttatat ttttataaat tattgacaaa
    11401 ttctttaatt tatattaaaa ttgaaaatgg ttacagagtt at
//
LOCUS       DASFSW010000066        11353 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.066, whole genome shotgun sequence.
ACCESSION   DASFSW010000066 DASFSW010000000
VERSION     DASFSW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11353
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.066"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..330)
                     /locus_tag="U1099_001252"
     CDS             complement(<1..330)
                     /locus_tag="U1099_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEM4867572.1"
                     /translation="MGMKKAALAYQKKGFSVIPISPSNKQPMIKFADKPAMTAQEIED
                     FWSQYPDSNIAVRTDKFFVIDIDLHGKHNGYESLANWEHLNLITPTLQAKTASGGKHI
                     FYFKHPDV"
     gene            complement(341..739)
                     /gene="ssb"
                     /locus_tag="U1099_001253"
     CDS             complement(341..739)
                     /gene="ssb"
                     /locus_tag="U1099_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM4867573.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQDGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRGQQSNSQDGSFGNSSPMDIQDEDLPF"
     gene            complement(736..1032)
                     /locus_tag="U1099_001254"
     CDS             complement(736..1032)
                     /locus_tag="U1099_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867574.1"
                     /translation="MGFTELGRKRPIKIQGDTYDEILIYKYKGEVWGVCYKGREIDCV
                     YNYTKEDFFYSRIWGMTLKEIVKKIIQPLKRTEPGIFAMIDYELVRKALKVEKL"
     gene            complement(1032..1730)
                     /locus_tag="U1099_001255"
     CDS             complement(1032..1730)
                     /locus_tag="U1099_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEM4867575.1"
                     /translation="MTVKESPIFATLCSIQTELVAPKGQYNSFGKYNYRSAEDILEAL
                     KPLLKKHDASLVLNDDIEQIGDRYYVRATVTLFACGSSISAQASAREEDTKKGMDGSQ
                     ITGTASSYARKYALNGLFAIDDNKDPDTDEYANQTGKQAQAQKAAQSKSTSNPKQNQN
                     ASGSVKYITGAQAKKLREDIKNIAEASGGPVNTVGVWFIGQLGVDKIESIPADRLKEA
                     QDLIAKTKKAKGME"
     gene            complement(1740..2059)
                     /locus_tag="U1099_001256"
                     /pseudo
     CDS             complement(1740..2059)
                     /locus_tag="U1099_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984443.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2080..3252)
                     /locus_tag="U1099_001257"
     CDS             complement(2080..3252)
                     /locus_tag="U1099_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867576.1"
                     /translation="MEIELRKWQAEAVKRSDRDCPGIFLEGLGGRGKTICAFEIAKHK
                     GAKKVLVLNNRLSILNGWIDTHNKLYSQDFRLEAMTDKRMQNIVANGEVLEFDVLIVD
                     EWQNMSSEANYKAYKKIRRDYSIGLSATPIRKKGQNFYPLEKTIFGQAEPNNKFDWQK
                     QHGKMKYDRFSYSKEKWEDFRDYESYVSGLPNFFRWEEIEEIEGAEENNGFEVIFEPM
                     WCLPANPEELDQLRRVNIVGKNGKYAMAKQSFGRKTFERYLTQTGFEVDFPKLKAVNA
                     DTPMLLQLDILLAKQSEMLIVSKSKQIVEVIYERHPEIGIWTGDKKEGHDRTNMVATS
                     QVLGVGVDGLQHRFKTIVVLDPSQPSDGDYDDYRQLLWRITGSRQQHDVRVVEFYF"
     gene            complement(3221..3685)
                     /locus_tag="U1099_001258"
     CDS             complement(3221..3685)
                     /locus_tag="U1099_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867577.1"
                     /translation="MRILAIDPSSNRIETSTTGIVLLDNAGLVSYWVVPFGARNFSRW
                     FREVGRDLEYDVVIVEEYQVRDNDYSRDNSVAETVEAVQACFPNVELVRNAGYVSDIP
                     DQLLRKLGLWTFDKSHHQDVRAAARLALFWAQRKDIEEVIQDIGNRITQMAS"
     gene            complement(3666..3911)
                     /locus_tag="U1099_001259"
     CDS             complement(3666..3911)
                     /locus_tag="U1099_001259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867578.1"
                     /translation="MMGTLFEQRPRFEQLSTPGYRIALMANELTDLFGISFKEALKCI
                     ELDHKINDYDVKDGQLAGFGELLRDYLEVKQNENPSN"
     gene            complement(3908..4387)
                     /locus_tag="U1099_001260"
     CDS             complement(3908..4387)
                     /locus_tag="U1099_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEM4867579.1"
                     /translation="MASIYELTGIFKQIAEMEGIDEETKLDTLESIDWTEQFEEKVEN
                     TVKVIKNKEADVDQLKEEIDRLTKRKKSIENDITRLKTGLQGAFEITGHDKIKTLLFT
                     VSLANNQPSVVVDEDLLPKKYFIQTLKPDKTAIKELLKAGKKVKGAVLQESRSLRIR"
     gene            complement(4387..4728)
                     /locus_tag="U1099_001261"
     CDS             complement(4387..4728)
                     /locus_tag="U1099_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867580.1"
                     /translation="MTQAERIREYYKQHPAASYDEVAEALKTSNSNVRANVSKDIKAG
                     RCVRLEDKSLDYSMHYIKNEALADLINWKNDTRREWVDMLTRAAEKETDNNTMRLLIK
                     EANKLMKEVTE"
     gene            complement(4733..4885)
                     /locus_tag="U1099_001262"
     CDS             complement(4733..4885)
                     /locus_tag="U1099_001262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867581.1"
                     /translation="MQDNHTTIEISVEEYIQLRNKVNDLQTENRFLRTIVDSVAVVMK
                     NSGMVR"
     gene            complement(4875..5123)
                     /locus_tag="U1099_001263"
     CDS             complement(4875..5123)
                     /locus_tag="U1099_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867582.1"
                     /translation="MTEAIMTLSLFAVPLLAVGIVEHRKAEKRRMHKEFEEIRRRDYL
                     YGFKAGMGYQSTCDTEKARNGLKRDAQQVDKEMARYAG"
     gene            complement(5164..5418)
                     /locus_tag="U1099_001264"
     CDS             complement(5164..5418)
                     /locus_tag="U1099_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867583.1"
                     /translation="MDKIFVELSDWIKSIIRDVVNEILLEKDNDDGFPEMMNRKDCIR
                     FLKVDGTVFDKYRKLPNFPKEQEGTKWKKRAIKIWLSEKD"
     gene            complement(5492..5818)
                     /locus_tag="U1099_001265"
     CDS             complement(5492..5818)
                     /locus_tag="U1099_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867584.1"
                     /translation="MVENLLGIPCGHNPVVLGSVVDGNTKIFGEIVTVFSGDIIFGNL
                     KKLDVYTEGEILKLSDLYDKYKDYISPNDSLYVVVTNGLSGTIYDCGHTRRGIWHIFA
                     KTAGYA"
     gene            complement(5888..6088)
                     /locus_tag="U1099_001266"
     CDS             complement(5888..6088)
                     /locus_tag="U1099_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867585.1"
                     /translation="MAKPKITIAELRAKNNKMSQKELASKIGVAYQTIGAWEDDITVI
                     KGDNLLKLCEFFGVSSSDLLGR"
     gene            6273..6653
                     /locus_tag="U1099_001267"
     CDS             6273..6653
                     /locus_tag="U1099_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867586.1"
                     /translation="MADAKIKYPEVGQRIRELREMRGFEQLDIANQLGYKSQSTISKW
                     ESGVNLPTGKKLILLAEMLDTSTDYILHGKISDTSTKDTTPTIDFKEMAAESMSYDGM
                     PLNDEDIDLIASILETRMKNRDKE"
     gene            6660..7043
                     /locus_tag="U1099_001268"
     CDS             6660..7043
                     /locus_tag="U1099_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM4867587.1"
                     /translation="MMTPESVCAERGIDLVYFDGRDSDKKGIYNKRANMIAVDAYLDE
                     IQHKKVIYHELGHENHDPAQYDRRREQYELQADRNMIHYLVKEELSLMDDVKEFNYVH
                     FMEKYDLKTIVNETMVIEEFNNLVN"
     gene            7091..7822
                     /locus_tag="U1099_001269"
     CDS             7091..7822
                     /locus_tag="U1099_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867588.1"
                     /translation="MKKNNFVKIILIIFAIALVVAIAAMLMPVAFVGGLGAVWYFTKK
                     KPDSNKRNISIAFAVVGLLGSIFITPAMLNNQKNTSTTVATAPSISSTSSTETSTSTS
                     STTEASSSTEESTEPTTKNDGPEYTEESNAAFATAFMNALNQSLADNGVDINVRVQYY
                     GKNLIHVIVPQDFKYEPNTSIQKVADSVFQAKENYFTEWAIDNGYDLGYTYAPKLYIN
                     AEDDTTLAEEEGILERKMELKIDNN"
     gene            8019..9176
                     /locus_tag="U1099_001270"
     CDS             8019..9176
                     /locus_tag="U1099_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4867589.1"
                     /translation="MKYTKTKYPNIFTYETQKGLRYYVRRGYFVNGDKKEFSKSGLRS
                     LKDAQRILRDIEERIYHDEMDVNLELTLNEYWEIYSAKKEKTGQWNDTSIYTNAGIYR
                     TMIKEKWGNLPLKKINRNDYEEHLAEMLGQYRRNSVLTSNRLLNSILNDAVKNGNLRQ
                     NKLSGIYLGESELEPLNKELKIDDFQTWIKTAEELLSTTHFAFVYLTIFGLRRGEVCG
                     IRFMDVTFDSNNRAVLNIRDSRSNRTKDGAGRTKTESSVRYVVLNDRGSELLLQIMKT
                     AKQVKKKADVIVKQEKDYLSIRIKNNKYYLERPAFLNKIFKRVSEECGIYITPHIMRH
                     FFTTQSLIAGARPEDVMHFLGHASLQTTKQYTHIKEERAHNVTDLFDKKVL"
     gene            9406..10332
                     /gene="cysK"
                     /locus_tag="U1099_001271"
     CDS             9406..10332
                     /gene="cysK"
                     /locus_tag="U1099_001271"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEM4867590.1"
                     /translation="MAIYQNITQLVGKTPVIKLNNIVPEGAAEVYVKLEAFNPGASVK
                     DRIALAMIEDAEKAGTIKPGDTIVEPTSGNTGIGLAWVGAAKGYKVIIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAKEIAEEKNGWVPFQFANPSNPKVHEDTTGQE
                     ILEDFGTTGLDAFVSGVGTGGTVSGVSHVLKTANPDIAIYAVEADESAVLSGEAPGPH
                     KIQGISAGFIPDTLDTSAYDGIIRVKSDDALATGRAIGGKEGFLVGISSGAAIHAAIE
                     VAKELGTGKKVLAILPDNGERYLSTALYEFND"
     gene            complement(10359..10730)
                     /locus_tag="U1099_001272"
     CDS             complement(10359..10730)
                     /locus_tag="U1099_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEM4867591.1"
                     /translation="MKIGDKLKGTVTGIQPYGAFVQLENGTTGLIHISEIKTGFVDNI
                     YDHLKLGQEVLVQVVDFDEYTEKASLSIRTLEEEQQKIPRHHRFTNERNKIGFSPLAK
                     QLPKWIEEAKGYLKEHKEKKV"
     gene            complement(10732..11337)
                     /locus_tag="U1099_001273"
     CDS             complement(10732..11337)
                     /locus_tag="U1099_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM4867592.1"
                     /translation="MVEKEKYFPQLDLENVTGPVAHIKTNHGKLTVKLFPEIAPKTVA
                     NFVALSKDGYYDGIIFHRIIKDFMIQGGDPTGTGMGGESIYGTAFEDEFSMEAFNLRG
                     ALSMANAGPNTNGSQFFIVQNQNFPYNAKELERGGWPKEVAEAYVKNGGTPHLDQRHT
                     VFGHLVDEESFVVLDAIAAVATDSADRPHEDVVIETIEIED"
BASE COUNT         3257 a         2455 c         2089 g         3552 t
ORIGIN      
        1 aacatctgga tgcttaaagt aaaagatatg cttgccaccg ctggcagttt ttgcttgcaa
       61 agtcggggta atcaagttga gatgttccca gttggccaga ctttcatatc cattatgttt
      121 tccgtgcagg tcgatgtcga ttacgaagaa tttgtcagtc cggacagcaa tattactatc
      181 cggatactga ctccaaaaat cttcaatttc ctgtgcggtc attgctggtt tatcagcaaa
      241 ttttatcatc ggttgcttat tagaagggct tataggaatg accgaaaagc cctttttctg
      301 ataagccaaa gccgctttct tcatacccat ctagcacctc ctagaacggc aaatcttcgt
      361 cttggatatc cataggactt gaatttccaa atgatccgtc ttgagaattc gactgttgtc
      421 cacgactttc caagagttgg aaactttctg caactacctc agtaacgtag acacgttgcc
      481 cttgttgatt gtcatagcta cgagtctgaa tgcgacctgt aacaccaatc agagcacctt
      541 tcttagccca attcgccaaa ttctcagctt gttgacgcca aatgactacg ttgataaagt
      601 ccgcttcacg ctccccatct tgatttttga agtttcgatt gacagccaag gtaaaagtcg
      661 caaccgcctg gtttgacgga gtgtaacgaa gttctgcatc acgagtcatg cgtcccacta
      721 gtactacatt gttaatcata atttttccac cttcaaggcc tttctcacca attcgtaatc
      781 aatcattgca aaaattcctg gctcagttct ttttaatggc tgaatgattt tcttaacaat
      841 ttctttcaaa gtcatacccc aaattcttga gtaaaagaag tcttctttcg tgtaattata
      901 aacgcaatca atctctctgc ctttataaca tacgccccaa acttcgcctt tatatttgta
      961 aatcaagatt tcgtcatagg tatcaccttg aattttgatt ggacgttttc gcccaagttc
     1021 cgtaaatccc attattccat tcccttcgct ttcttagttt ttgcaatcag atcctgtgct
     1081 tctttcaagc ggtctgctgg aatactttcg attttatcca cgcccagctg gccaatgaac
     1141 cacactccaa cggtattaac cggaccgcct gaagcctccg caatattctt gatatcttca
     1201 cgtagcttct tcgcctgggc tcccgtgatg tacttaacac tgccagatgc gttctggttc
     1261 tgtttcggat ttgatgtaga cttactttgt gctgccttct gagcttgtgc ttgctttcca
     1321 gtttggttcg catattcgtc cgtatcaggg tccttgttat catcaatggc aaacaagccg
     1381 ttaagagcat attttcgagc gtaactggat gcagttccgg tgatctggct gccatccatt
     1441 cctttctttg tatcttcttc ccttgctgac gcttgggcac tgattgaact gccgcaggca
     1501 aataatgtta ctgtagcacg aacatagtag cggtccccaa tctgttcgat atcgtcattt
     1561 aataccaggg atgcatcatg ctttttgagt aaaggcttta gtgcttccaa aatatcctct
     1621 gcactacgat agttgtattt tccgaaagaa ttgtactgtc ctttcggtgc aaccagctct
     1681 gtctgaatac tgcacagggt tgcaaatatt ggcgattctt ttactgtcat gtattctcct
     1741 taaagttgtt tgaatgcttc catcaaatca tcaaataaat ttgattcttt gggaataatt
     1801 ttagaaacct cttctccatt aggataagta agtgtatact cagcatctac caagatgatt
     1861 tcacaatcat gaattttcgc aagagcttta atttcggatt cttgtttcaa ataatactcg
     1921 tatggaagtg atagggcacc acggatttct tctacaagac ctgctttcgt tgcaagtgaa
     1981 tggggattat ttttgatttc tgacataaaa tacccatttt tttctcacgc aatacgatta
     2041 acttgtttga aattttcatt tttttgctcc ttttgttttt taaaaataaa attctacgac
     2101 cctaacgtcg tgttgttgac gactaccagt aatccgccaa agaagttgcc gatagtcatc
     2161 gtaatctccg tcactaggct gggacggatc cagaactaca attgttttaa aacggtgctg
     2221 gagcccatcg accccaactc ctaatacttg gctagtggcg accatattgg tcctgtcatg
     2281 cccttctttc ttatcaccgg tccaaatacc gatttcaggg tgccgttcat agataacctc
     2341 aacgatctgc ttggacttgc tgacaattaa catttccgat tgcttggcca agagaatgtc
     2401 tagttgaagc aacattggtg tatctgcatt aaccgcttta agcttcggaa aatcaacttc
     2461 aaatcctgtc tgagtcagat acctctcgaa agtcttgcgg ccaaatgact gcttggccat
     2521 tgcatactta ccattctttc cgacgatgtt cacccgtcga agttgatcaa gttcttctgg
     2581 atttgctggc aggcaccaca tcggctcaaa gatgacttca aatccattgt tttcctcagc
     2641 tccctcaatt tcctcaatct cttcccaacg gaagaaattt ggcaaccctg acacataact
     2701 ttcatagtca cggaagtctt cccacttttc tttggaataa ctgaatcggt catacttcat
     2761 cttgccgtgc tgtttctgcc aatcaaattt attgtttggc tccgcctggc caaagattgt
     2821 tttttctagc ggatagaagt tttgaccttt ctttcggatt ggagttgcag acaagccgat
     2881 tgaatagtca cgtcggattt tcttgtaagc tttatagtta gcttcactgg acatattctg
     2941 ccactcgtca acgattaaga catcaaactc caggacctca ccgtttgcca cgatattctg
     3001 cattcgcttg tctgtcatgg cttctagtcg aaaatcttgt gaatagagct tgttgtgagt
     3061 gtcaatccag ccattcagga ttgacagccg attgttcaga accaagacct tcttggctcc
     3121 cttgtgcttt gcaatctcaa aggcacagat agttttccca cgaccgccaa gaccttccag
     3181 aaaaatacca ggacaatcgc ggtcgcttcg tttcacggcc tcagcttgcc atttgcgtaa
     3241 ttcgatttcc aatgtcttga ataacctcct cgatgtcttt tctctgcgcc cagaatagtg
     3301 ccaagcgagc agcagccctc acatcctgat gatgtgattt gtcaaaagtc caaagaccaa
     3361 gcttcctaag taactgatca ggaatatctg acacatagcc agcgttgcga acgagttcca
     3421 cgtttggaaa gcaggcttga acagcttcca ctgtttcagc aactgagtta tctcgagaat
     3481 aatcattgtc acgtacctgg tattcttcta caatgacaac atcgtattcc aggtcacgac
     3541 caacttcacg aaaccaccga ctgaaatttc tggcaccaaa tgggaccacc caatagctga
     3601 ccagtccggc attgtccaac agcacaattc ctgtagtgga tgtttcaatt ctgttgcttg
     3661 acggatcaat tgctaggatt ctcattttgt ttgacctcta ggtagtctct caacaactcc
     3721 ccaaatccag ctaattgtcc atcttttacg tcatagtcat ttattttgtg atcgagttca
     3781 atacatttta aggcttcctt aaagctaatc ccgaacaggt cagttaattc attagccatt
     3841 aaagcaatcc tataccctgg tgttgacaat tgttcaaatc ttggcctttg ttcaaataat
     3901 gttcccatca tctaatcctc aaacttctac tctcttgcaa cactgcaccc ttgaccttct
     3961 taccagcttt cagcaactct ttgatagctg ttttgtctgg tttcaaagtt tgaataaaat
     4021 atttcttagg cagcaggtct tcatctacaa ccacagatgg ttgattattg gccaagctga
     4081 cagtaaacag caatgttttg attttgtcat gccctgtgat ttcaaatgca ccttgcagcc
     4141 ctgttttgag tcgtgtgatg tcattttcaa tagatttttt tcgtttggtt aagcggtcga
     4201 tttcttcttt gagttgatcc acatctgctt ctttattctt gatgaccttg acggtgtttt
     4261 caaccttttc ttcaaattgt tccgtccagt caatagattc aagcgtatcc agcttggttt
     4321 cttcatcaat accttccatt tctgcaatct gcttaaagat tccagttagt tcataaatac
     4381 tagccattat tccgttacct ctttcattag tttattagct tccttgataa gcaatcgcat
     4441 ggtattgtta tcagtttctt tttctgctgc tcttgtcagc atatcaaccc actccctcct
     4501 ggtgtcattc ttccagttga ttaggtctgc caatgcttca ttcttgatgt agtgcatcga
     4561 gtagtccaat gacttatctt ccaagcggac acaccgtcca gccttgatat ctttggacac
     4621 gttcgcccgt acattactat ttgatgtttt gagtgcttca gccacttcat catagctagc
     4681 agctggatgt tgtttataat attccctaat gcgttccgct tgtgtcatct tcctacctca
     4741 ccattccact atttttcatc acaaccgcca cagaatcaac aatcgttctc aaaaaacgat
     4801 tttctgtctg caagtcgttt accttgttcc gaagttgaat atattcttca acgctgattt
     4861 caattgttgt atgattatcc tgcataccgt gccatctcct tatctacttg ctgagcgtct
     4921 ctctttagcc cgttacgagc tttttcggtg tcacaggtac tctgataccc catgcccgcc
     4981 ttaaagccgt acaggtagtc tctgcgacga atttcttcaa actctttgtg catccgtctt
     5041 ttctctgcct tccgatgttc cacaatgcct actgccagca aaggcactgc gaataaactt
     5101 aatgtcataa ttgcttctgt cataaatttt ttcctcgttt cagaccttgt ccagattggt
     5161 cttttagtct ttttcgctaa gccaaatttt aatagctctt ttcttccatt ttgttccctc
     5221 ttgttctttg gggaaattcg gtaattttct atatttatca aagacggtcc cgtctacctt
     5281 tagaaatctg atgcaatcct ttcggttcat catttccggg aaaccatcgt cgttgtcttt
     5341 ttccagtaaa atctcgttta caacgtctct tattatcgat tttatccagt cggacaactc
     5401 aacaaatatc ttgtccatag tagcctcctg tgttataatt tgagtaagtt aattttgtaa
     5461 gccactgttc ccgcagtggt ttttttgcgt gtcaggcata acctgcggtt tttgcaaata
     5521 tatgccatat cccacgtctg gtatgtccgc aatcgtaaat agttccagat aagccattag
     5581 tgacaacgac atataaacta tcgttcggcg agatatagtc tttatactta tcgtacaaat
     5641 cagataactt taaaatttca ccttccgtgt atacatccaa ttttttcaaa tttccgaaga
     5701 taatatcgcc actaaacaca gtcacgattt caccgaaaat cttggtattt ccgtcgacca
     5761 cactacctaa aacaactgga ttgtgtccgc atggaatccc tagcaagttt tcaaccatcc
     5821 cttctccttt ctagtttgtt agttgggtag tttctacatg ataaaaaatc atgtattttt
     5881 taaaaaatta acgtccaagt aaatcagacg aacttactcc aaagaattcg cacaatttta
     5941 atagattatc gcctttaatg actgtaatgt catcttccca tgctccaatt gtttgataag
     6001 caacaccgat ttttgaagca agttctttct ggctcatctt attattcttt gcacgaagtt
     6061 ctgcaattgt gatttttggt tttgccattc agttctcctt tcacatgatt ttaagtcatt
     6121 tccttgacct tgactatatg atacatgatt ataaatcatc tatcaagcgt ttttttgatt
     6181 ttttttcatc tttttttggt atttgcacaa aatcacttga tataaaatca ctttttgctt
     6241 ataatatacc tatgaataga gaggtaaaaa ccatggctga tgcaaagata aaataccctg
     6301 aagtagggca aagaattaga gagctgagag aaatgagagg gttcgagcaa ttagacatag
     6361 ctaatcaact tggttacaaa tctcaaagca caatttcaaa atgggagagc ggagtgaatt
     6421 tgccaactgg caaaaaacta attttattgg ctgaaatgct agatacttcc acagactaca
     6481 ttcttcatgg aaaaattagt gacacttcca caaaagacac cactcccaca atcgatttca
     6541 aggaaatggc ggcagagtcc atgtcttatg acggcatgcc tctcaatgat gaagatatcg
     6601 acctcattgc ctcaatactc gaaactcgca tgaaaaacag agataaggaa taattgccta
     6661 tgatgacacc agaatcagtc tgcgcagagc gtggaattga tttggtctat tttgacggta
     6721 gagattcgga caagaaaggc atatacaaca agcgtgcgaa catgattgcg gttgacgcct
     6781 atttggacga aatccaacac aagaaagtca tctaccacga attgggccat gaaaatcacg
     6841 acccggcaca atacgaccga agacgtgaac agtatgaatt acaagcggat aggaatatga
     6901 tccattatct ggtcaaagaa gaattgtctt tgatggacga tgtcaaagaa tttaattatg
     6961 ttcactttat ggaaaaatac gacttaaaaa ccatagttaa tgagactatg gtgattgagg
     7021 agtttaataa cttagttaat taaaaatacg tgcacgccca ccgcacgaaa aacgggcagg
     7081 aggttgtttt atgaagaaga ataacttcgt aaaaataatt ttaattatct ttgcgatagc
     7141 gctggtagta gcaattgctg caatgttaat gcctgtagca tttgtaggtg gtcttggagc
     7201 agtttggtat tttactaaaa agaaacctga tagtaataaa cgcaacatct ctatcgcttt
     7261 tgctgtagtt ggattgttag gaagcatatt tattacacct gcaatgctaa acaaccaaaa
     7321 gaacacctca acaacagtag caactgcgcc ttcaatcagc tctacaagta gcacagagac
     7381 ttctacttca acaagtagta ccacagaagc ttcttcgtca actgaggaat cgacagagcc
     7441 gacaacaaaa aatgacggtc cagaatatac cgaagaatct aacgcagctt ttgcaacagc
     7501 atttatgaac gctctcaatc aatcccttgc agacaatgga gtagatatta atgttagagt
     7561 gcagtattac ggaaaaaatc tcattcatgt aattgtgcct caggatttta aatatgaacc
     7621 aaatactagt atccaaaaag tagcagatag cgtcttccaa gccaaagaaa attactttac
     7681 agaatgggct attgacaacg gctatgacct tggatacaca tacgctccta aactatacat
     7741 aaacgccgaa gacgatacta cgctggctga agaagaaggt atcctagaaa gaaaaatgga
     7801 attgaaaatc gacaacaact aatatttaaa cgtcgataaa taaaaaaatc cccacacttc
     7861 caaccgacca aagccgaagt gtagggtagt ccaataagca aagaccagct tttcagcagg
     7921 actttttgcg tactctaatt atatcacttt aaggaggtga tgccaatatc ctttcaaaaa
     7981 aaccttgtcc agattggtct tacagaaagg aaaagagaat gaaatataca aaaacaaaat
     8041 acccaaatat ttttacatac gaaacccaaa aaggattgcg ttactatgtc cgcagaggat
     8101 actttgttaa tggcgacaag aaagaattta gcaagagcgg actacgaagc ttgaaggacg
     8161 cccagagaat tctaagggat attgaagaaa gaatttatca tgatgaaatg gatgtcaacc
     8221 tagaactgac tttaaatgaa tactgggaaa tctactcagc aaagaaagaa aaaacaggtc
     8281 aatggaatga tacatccatc tacactaacg caggcattta tcgtaccatg ataaaagaaa
     8341 agtggggaaa cctcccgcta aaaaaaataa atcgcaacga ttatgaagaa catctggcag
     8401 aaatgcttgg ccagtaccgc agaaatagtg tgcttaccag taatcgactt ttgaattcca
     8461 ttttaaacga tgctgtcaaa aatggaaatc ttagacaaaa taaattgtct ggtatttatc
     8521 ttggggaatc agagttagaa cctcttaata aagagttaaa aatagatgat tttcaaacat
     8581 ggataaaaac cgcagaagaa ttactatcga ccacacattt tgcatttgtc tacctgacta
     8641 tttttggact tagacgcggg gaagtctgtg gtatcaggtt catggacgtt actttcgatt
     8701 cgaataatcg agcagtctta aatataagag actctcgttc caacaggaca aaagatggtg
     8761 ctggaagaac aaaaacagaa agttctgtgc gttacgttgt tttaaatgac agaggcagtg
     8821 agctgttgtt acagatcatg aaaacagcaa aacaagtcaa aaagaaagca gacgtcattg
     8881 ttaagcagga aaaggattac ctgagcatcc gaataaagaa taataaatac tatttggaaa
     8941 gacctgcttt tttaaacaaa atttttaaac gagtaagtga ggaatgtggt atctatatca
     9001 ctccacacat tatgcgtcac ttctttacca ctcaatccct tattgctgga gccagaccag
     9061 aagatgtcat gcacttcctg ggtcatgcaa gccttcaaac aaccaaacaa tatactcaca
     9121 tcaaagaaga acgagcacat aatgttaccg atctatttga caagaaagtt ctataatttt
     9181 ccccgacttc ttcccgacaa ttccccgaca actacaccaa gttatgacaa tatcaacaat
     9241 aaacattttt tcaaaaagct agtagtatca acgttttcaa gttgttacat aaataccatg
     9301 attggctggg tatagctttt tcttaacggc gagatagttt ttttatatta tccttttttg
     9361 actttctact atataatgta taataacatt atttggaggt gccatatggc tatttatcaa
     9421 aacattactc aattagttgg aaaaacacct gttattaaac tcaataatat cgttccagag
     9481 ggggcagcgg aagtctatgt gaaattagag gcctttaacc caggagcttc tgtaaaagac
     9541 cggatcgcct tggctatgat tgaagatgct gagaaggctg gtacaatcaa acctggagat
     9601 actattgttg agccaacaag tggtaacact ggtatcggtc ttgcttgggt tggagcagca
     9661 aaaggttata aggtgattat cgtcatgcct gaaactatga gtgttgagcg tcgtaagatt
     9721 atccaagctt atggagctga gcttgtcttg actcctggta gcgaagggat gaagggggct
     9781 atcgccaaag caaaagagat tgctgaagaa aaaaatggct gggttccttt ccagtttgct
     9841 aatccatcca atccaaaagt tcacgaagat acaacaggac aggaaatttt ggaagacttt
     9901 ggaacaactg gtttagatgc ctttgtatct ggcgttggta ctggtggtac cgtttcaggt
     9961 gtttcgcatg ttttgaagac agcaaatcca gatattgcta tctatgctgt cgaagctgat
    10021 gaatcagctg tcttatctgg tgaagcacct ggtccgcaca agatccaagg tatttcggca
    10081 ggttttattc cagatacttt ggacactagc gcctatgatg gcattattcg tgtcaaatcc
    10141 gatgatgcct tagctacagg tcgtgctatc ggtggaaaag aaggattctt agttggtatt
    10201 tcttcaggtg cagcaattca cgctgctatc gaagtcgcta aggagttggg aactggaaaa
    10261 aaagtgttgg caatcttacc tgataacgga gaacgttact tgtcaacagc tctttatgag
    10321 ttcaatgatt aaaaaagaaa gaaggttagg aatgcatcct ataccttctt ttctttgtgt
    10381 tctttgagat agcctttagc ttcctcaatc catttcggaa gttgttttgc taaaggggaa
    10441 aaaccaattt tattccgttc atttgtaaaa cgatgatgac gcggaatttt ttgttgttct
    10501 tcttccaagg tccggattga taaactagcc ttttcagtat attcatcaaa gtcaactacc
    10561 tgtaccaaaa cctcttgccc caattttaaa tgatcataaa tattatcaac aaaacctgtt
    10621 ttgatttccg agatatggat taggcccgtc gtcccattct ctaattgtac aaaggctcca
    10681 tacggttgaa tacccgttac cgtccccttg agtttatctc cgattttcat cttaatcctc
    10741 tatttcgatg gtctcaatta caacatcttc atgtggacga tctgctgaat ctgttgcaac
    10801 cgctgcgata gcatccaaga cgacgaagga ttcttcatca actagatgac caaacactgt
    10861 gtggcgttga tccagatgtg gtgtcccgcc atttttgaca taggcttctg caacttcttt
    10921 aggccaaccg cctcgttcta attctttcgc attgtatggg aaattttggt tttgaacgat
    10981 aaagaattgg cttccatttg tatttggtcc tgcattggcc attgacaagg ctcctctgag
    11041 attaaaagct tccattgaaa attcatcttc aaacgcagta ccataaatag attcaccacc
    11101 catacctgtt ccggttggat cgccaccttg aatcataaag tctttgataa tacgatgaaa
    11161 gataatcccg tcataatagc catctttaga aagagctacg aaatttgcta cagttttggg
    11221 tgcaatttca ggaaagagtt ttactgttaa cttaccatgg ttagtcttga tatgcgcaac
    11281 tggtcctgta acattttcca aatctaattg cgggaaatat ttttcttttt ctaccatacc
    11341 taatttctcc aag
//
LOCUS       DASFSW010000067        11322 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.067, whole genome shotgun sequence.
ACCESSION   DASFSW010000067 DASFSW010000000
VERSION     DASFSW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.067"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            141..1415
                     /locus_tag="U1099_001274"
     CDS             141..1415
                     /locus_tag="U1099_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEM4867593.1"
                     /translation="MKKIGRYVTLLAAAGLLAACSTSTESSKTTEGSSSGKTEISYAI
                     WDSGQEPGLRKIADEFEKKNPDIKINIQVSDWDSYWTMLEAGATGGSLPDTFWMHSNE
                     IYRYGSNEMLLPLDEYLAKSEDAKLANFPDGLNEIYNINGKQYAIPKDFDTIGLWYNK
                     KLFDEAGIPYPDDTWDWNKLKEVAKKLTKPDGSQYGFGAGLSNQEGYYNFIYQNGGKV
                     ITDDLKSGYADPKTIEALDYYFSFVKEKISPAITVDKERAEAFQNGQVAMSVFGSWNL
                     SGFTANDYIRENADVAVLPKGPDGTRATIFNGLGHAIAATTKHPDAAWKWVEYLSSKE
                     AQEMQATLGVAISAYKGAADTWVDSNKNFTIKNYVDMVDYAQIRPYSQTTIKWEDKAY
                     ELLKPAYLGEKATEEAAKETADMMNAELATEK"
     gene            1484..2377
                     /locus_tag="U1099_001275"
     CDS             1484..2377
                     /locus_tag="U1099_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM4867594.1"
                     /translation="MFRKKGSLNEAIWGWGMVAPTIIGLIVLNIIPIFQTMKMSFHKS
                     GDFGKGDIFVGLANYQRMLGDAQVWQATWNTLKYTILVVPATVALAMLLAVLLNSKIK
                     GKHIYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINYILRRLGLGAVNWIEDPKIALY
                     SIALIGIWSTVGYSMILILAGLQEIPTDFYEAARIDGASPAKQFFSITLPLVSPTLFF
                     VVVTSVIQSMQVFDVIYMMEDVRSPAYDKTVSLVYLFYNNSFKYSDKGYGSTIVMLLL
                     LIILVITFVQMKVQKKWVHYR"
     gene            2388..3218
                     /locus_tag="U1099_001276"
     CDS             2388..3218
                     /locus_tag="U1099_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM4867595.1"
                     /translation="MKQSAFKSTIWMHAFLILVAMGMLIPFVWMVLTSFKTVTESTQM
                     NPFHFLPSNWMVSNYTEAIRTNNFPILYLNTILMMLWRIFSSVMFSAMAAYAFARLEF
                     PGRNFLFGLVLFQMMVPPQLFVIPQYLMIDQLGMRNTIFALVFPGIVSAFGTFLLRQF
                     FMGLPKELEESAKLDGCNIGQTFFKVMLPLAKSGLIALAIFTALFAFKDLLWPLIINS
                     EADKATLSSALSKIQGAYAVNYPQLMAASVLAIWPMLVLYVVFQKQFIQGIATSGGKL
                     "
     gene            3228..5429
                     /locus_tag="U1099_001277"
     CDS             3228..5429
                     /locus_tag="U1099_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEM4867596.1"
                     /translation="MGIIFHEKTKEFHLYNEFISYVLTVLPNGHIGSLYYGKRVTETA
                     SYQYVREDEYRALTSFVEDDDRFFSLQYANPEFACYGTTDYFSPTFELVQKDGSSLSH
                     FVYQGHVIYAGRSALKGLPHLYLDEEGQADSLDIYLLDEESQTRLVLSYTIFRDYPAV
                     TRSARFEQLGEQSVCLNRALSMTLCLPDMDYDWLHLDGAWGRERHLQLSPLHQGCQSI
                     YSLKGASSAEHNPFMALKRPTADEQQGEVLGFSLVYSGNFLAQVDVSSFQKTRVSMGI
                     HPERFSWTLDKGEEFQTPEVVMVYSEHGLNGMSQTYHRLFQKHLVRGYWRDRERPVLL
                     NNWEAMSFDFDEETILSLAKEAAGLGVELFVMDDGWFGKRNHDRAGLGDWTVNREKLP
                     SGLTGIIDQVHAMGMKFGLWIEPEMVNKDSDLYRAHPDWIFHHPQHSQSHGRHQYTLD
                     LSREDVYQNIHDQLHRLLAEHDIDYIKWDMNRYMTEVFTCTREAERQGETFHRYILNL
                     YRLYDSLIAAFPTVLFESCSSGGARFDPGMLYYAPQTWVSDDTDAMERLKIQYGTSMV
                     YPLNSTGCHVSASPNQQLGRATPLSTRANVAFFGSFGYELDLFECSQEELEEMKEQIA
                     FYKAHRKLFQQGIFTRLKSPFDGEITAWQVQSLDGQQVIVGYYRRLTTANLSYQRLYL
                     QGLEEEAVYHLDGEVYTGSQLMHTGLSIRHGDHVGDNKDFTSYLMVLQKQQ"
     gene            5704..6405
                     /locus_tag="U1099_001278"
     CDS             5704..6405
                     /locus_tag="U1099_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM4867597.1"
                     /translation="MKKYAFREGMRDAIPTVLGYASIGLACGVVSVNSGISTVEMGLM
                     SLLIYAGSAQFVMCAMIFAGAPLLSIAVTVFFVNLRNFLMCLHATTIFQGNRLGSNIL
                     IGSFVTDESYAVLLRKQIEHHTITPEWMYGNNFASYASWVTFTTLGNLIGGLIPNPEQ
                     FGLDFALVAMFVGIFAGQLEAMVRTIPLKKIGLILLAVGLTYIGLSMMVSSYVAVLLA
                     TLLGCFVGVMIDDKN"
     gene            6392..6715
                     /locus_tag="U1099_001279"
     CDS             6392..6715
                     /locus_tag="U1099_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM4867598.1"
                     /translation="MIKTNILLIILMAALVTWVPRVLPFVLTQNKSLPPKLVKFLSFL
                     PITIIFALTLSSIMDEKVGSLPSFLPVESLAILPTFFVVLKTKNILLAVLVGVVTTAL
                     LRLIF"
     gene            6725..7747
                     /locus_tag="U1099_001280"
     CDS             6725..7747
                     /locus_tag="U1099_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEM4867599.1"
                     /translation="MVKENAKGVGLCFLLALVGQWLGHLFPLVGGPVFALLIGMSLYP
                     YFSSKNAFQSGLTFTSKKILQYAVICLGFGLNLSAVLAVGRQSLPIIFSTISFALILA
                     FLMWKILPISSHLATLIGVGTSICGGSAIAATAPIIQADDEDVAQAISVIFLFNVLAA
                     LVFPTLATWLGFSTDSGQVFGMFAGTAVNDTSSVTAAASTWDSLYGLGSQTLDTAVTV
                     KLTRTLAIIPITTVLAIWQTRGKGVQADKKSLLAGFPVFILYFILASLITTIAGGFGI
                     GADWFAPLKSLSKVFICMAMVAIGLRTNVFALVKNGRAALLVGLVCWFGVTALTLVWQ
                     AILGIW"
     gene            complement(8047..9672)
                     /locus_tag="U1099_001281"
     CDS             complement(8047..9672)
                     /locus_tag="U1099_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867600.1"
                     /translation="MKTILRYFWYQEKYNLYRTVNGFFYYLRKLPLVGKSIPESIFKS
                     YSFKSGLFLILHVLSIPSRFLVKGIWLALNFYLASFWINLLSGGDLTFWNILPGTWLL
                     GFSLWMVFVGYTYRFSKGFEPFIVKSEREFMQNFGLSQSSFLQSQLFVEPIITSLFYL
                     PALLIFSGLSGNWLYLPLGLLTIPTGSFTGQALNRWFFNRRILSRRNSWQSWVILGTG
                     LAAIACLILFRNHLSPIFLLPVLVCQVLLIWFGYGYLKQQTNHLDYLYYCMDQSLQMD
                     KKIFEMTKGNEYTRQGLQMQAKLSMETGKDLTHLSGMTYLNALLFDRYRSVLTKKLHF
                     RLGCLLAIVLFIEGIRWFSKDDIQISNTNYIEGLPFAFMVMYAASMGKAVAQMVFVNC
                     DISMLHYPFYREAKTIIAGFHYRFLQTVKLNAAFSGSLLLALIIFTRAQLPIETYLLT
                     ALLLLSLTALFSFHDLFIYYILQPFTKDMEVVNPVYKFLSGALYWVAYLNIKLDLGSH
                     MYILLISIAMITYVSIGYWILLKKAPQTFRLKS"
     gene            complement(9682..10380)
                     /locus_tag="U1099_001282"
     CDS             complement(9682..10380)
                     /locus_tag="U1099_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867601.1"
                     /translation="MQLKLSSVSKKFDHKIILEDTSFTFEQGKIYGLLGRNGAGKTTL
                     FNCIARNLTLDGGSIQFVKDEIAFDYDNTDIGFTQTYPQLPAFMTAYEFVRFYMDIHK
                     DKLKSPRSPEEWLNLVGIGEEDRHRLLKDFSHGMQNKVQLLLSLIVQPPVLLLDEPLT
                     SFDPVAAHEFKQLIREAKKDSVIIFSTHILQLAQDLCDEIVLLHHQELQAVPSDKLHD
                     PDFEQEVVTLLTAD"
     gene            complement(10461..11153)
                     /locus_tag="U1099_001283"
     CDS             complement(10461..11153)
                     /locus_tag="U1099_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867602.1"
                     /translation="MPINSFGQKIGMPLPHYSSGERPSLETIQGHTVRLEKLSLHHAD
                     DLYQFYGPSAKKTDWTYLPLEPFEDRATFQDYFQTLLNSTDPYYFAILDQATNQAVGT
                     FALMRIDTNHRTIEVGWVIFSPQLQRTRQATEAHYLLMTYVFEELGYRRYEWKCDSLN
                     QASRVAANRLGFTYEGNFRQALVYKERNRDTAWFSLLDHEWPDRKRALENWLKDSNFD
                     GQGQQKQPLSSF"
BASE COUNT         3073 a         2163 c         2731 g         3355 t
ORIGIN      
        1 tgaaaaaatt aaaaaatatc ccaaaatcat atggaatatg tcaaatattc ctatttgatt
       61 ataaagcgct tacatgttat agttatagta gaaataacaa agttcgtcga catattcgac
      121 aaaagaaaag gagtattatc atgaagaaaa ttggtcgcta tgttaccttg cttgcggctg
      181 caggcctgct ggcagcgtgt tctacttcga ctgagtctag caaaactacg gaaggaagta
      241 gttcaggaaa gacagaaata tcctatgcta tttgggattc tggtcaagag cctggtttga
      301 gaaagattgc ggatgagttt gagaaaaaga atccagatat taaaatcaat attcaagtat
      361 cggactggga ttcctactgg accatgttag aagctggtgc gacaggtggt tcattgcctg
      421 ataccttctg gatgcattca aatgagattt atcgctatgg ttccaatgaa atgctattgc
      481 cattggatga atacttagcc aagagtgaag atgctaaact agctaatttc ccagatggtt
      541 tgaatgaaat ctacaacata aatggtaagc agtacgctat tccaaaagat ttcgatacaa
      601 tcggtctttg gtacaataaa aaattatttg acgaagcagg tattccatat ccagatgata
      661 cttgggattg gaataagttg aaagaagtag cgaagaaatt gaccaagcca gatggtagtc
      721 aatatggttt cggtgctggt ttgagtaacc aagagggcta ctacaacttt atctatcaaa
      781 atggtggtaa ggttattact gatgatttga aatcaggcta tgctgatcct aagaccattg
      841 aggctttgga ttactacttt agctttgtca aagaaaaaat ttcaccggct attacagtag
      901 ataaagagcg ggccgaagca ttccaaaacg gtcaagtggc tatgagcgtc ttcggttctt
      961 ggaatttatc tggtttcact gctaatgact acattcgtga aaatgctgat gtagctgtct
     1021 tgccaaaagg tccagatgga actcgtgcaa ccatctttaa tggattgggt catgccattg
     1081 ctgctaccac taagcatcca gatgctgctt ggaaatgggt tgaatacctc agctcaaaag
     1141 aggcacaaga aatgcaggca acacttggtg tggcaatctc ggcttataaa ggcgcggcag
     1201 atacttgggt agattcaaac aaaaacttta ctatcaagaa ttatgttgac atggtggatt
     1261 atgcacagat tcgtccatat tcacaaacaa ccatcaagtg ggaagataag gcttatgagc
     1321 tattgaaacc tgcttatctt ggagaaaaag caactgaaga agctgccaaa gaaacagctg
     1381 atatgatgaa tgctgaatta gcaactgaaa aataggtagg aggtcttggg aagttgattc
     1441 ttccccggac cttcttatta aaatgaagaa ggatggggat aagatgttcc gaaaaaaagg
     1501 aagtttaaat gaggcaattt ggggatgggg aatggtagca ccgaccatta ttggattaat
     1561 cgtgctcaat attattccta ttttccaaac catgaagatg agttttcata agagtggaga
     1621 tttcggtaaa ggcgatattt ttgtcggctt ggcaaattac caacgaatgt taggggatgc
     1681 gcaagtgtgg caggcgacat ggaatacctt gaagtacacg attctagttg ttccagctac
     1741 cgttgccttg gccatgttgc ttgcagtttt gcttaattct aagattaaag gtaagcatat
     1801 ttatcgaacc attttcttct tgcctatggt ggcagcacca gccgcagtga ccatggtttg
     1861 gaaatggctc tacaatacgg acttcggctt gattaactat atcttgcgtc gtttgggact
     1921 gggtgctgtg aactggattg aagatccaaa gattgctcta tactcaatcg ctttgattgg
     1981 aatttggagt acggttggtt atagtatgat tttaatttta gctggtttgc aagaaattcc
     2041 aacggatttt tatgaggcag ctcgcattga tggagctagt ccggccaagc aatttttctc
     2101 cattaccctg cccttggttt caccaaccct gttctttgtt gtggtaacca gtgtcattca
     2161 atccatgcaa gtctttgatg ttatctatat gatggaggat gttcgtagtc ctgcctatga
     2221 taagacagta tctctggttt atctgtttta caataattcc tttaaatatt cggataaggg
     2281 ctatggctcc accatcgtca tgttgttgtt attaattatt cttgtcatta ctttcgttca
     2341 gatgaaggta cagaaaaaat gggtgcatta ccgttaagga ggttcatgtg aaacaatcag
     2401 cttttaaatc aacaatctgg atgcatgcct tcttgatttt agtggcaatg ggcatgctga
     2461 tcccatttgt ttggatggtg ttgacgtctt ttaaaacggt aaccgaatcc actcagatga
     2521 atcctttcca ctttttacca agtaactgga tggtgagcaa ttatacggag gctattcgga
     2581 ctaataattt cccgatactt tatctgaaca ccattttgat gatgttatgg cgtattttta
     2641 gctcggtcat gttctcagcc atggcagctt atgcctttgc tcgactggag tttcctgggc
     2701 ggaatttctt gtttggtctg gtcctctttc aaatgatggt gccacctcag ttattcgtca
     2761 ttcctcagta tttgatgatt gaccaacttg gtatgcgcaa tacgattttt gctctggtat
     2821 tcccaggaat tgtcagtgcc tttgggacat tcttgctccg tcaatttttc atgggtcttc
     2881 caaaagaatt ggaggaatct gcaaaattgg atggttgtaa tatcgggcag accttcttta
     2941 aggtcatgtt gcctttggcc aagtctggct tgattgcctt agcgattttt acggctttgt
     3001 ttgctttcaa ggatttgctg tggccattga ttatcaactc agaagcggac aaggctaccc
     3061 tgtctagcgc actttctaag attcagggag cttacgctgt taactatcct cagctcatgg
     3121 ctgcaagtgt tttggctatt tggccgatgt tggtactgta tgtggtattc cagaagcagt
     3181 tcatccaagg gattgcgaca tctggtggaa aattatagga ggctacaatg ggaattatct
     3241 ttcatgaaaa gacaaaggaa ttccatcttt acaatgagtt tattagctat gtgttgacgg
     3301 tcttgcctaa tggtcatatt ggtagtttgt attatggaaa gcgtgtaaca gaaacagctt
     3361 cctatcagta tgtgcgtgaa gatgagtatc gtgctttgac ttcctttgta gaagacgatg
     3421 accgtttctt ttcgctccaa tatgccaatc ctgaatttgc ttgttatggt acaacggact
     3481 atttttctcc gacttttgag ttggtgcaga aggacggtag tagcctgtcg cactttgtgt
     3541 atcaaggtca tgtgatttat gctgggcgat cggctttgaa gggtcttcct catctctatc
     3601 tggatgagga gggacaggca gatagtttgg atatttattt gttggatgaa gagtcgcaga
     3661 cccgcctggt cttgtcttat acgatttttc gagattatcc agcggtgact cgctctgctc
     3721 gttttgaaca actcggagaa caatctgtct gtttgaaccg tgccttgagt atgacacttt
     3781 gtctgcctga tatggactat gattggttgc atttggatgg ggcttggggg cgtgagcggc
     3841 atctgcaact atcgcctctt catcaaggtt gtcagtctat ttacagtctc aaaggggcta
     3901 gtagtgcgga gcacaatccc tttatggctt tgaaacgccc aactgcggat gagcaacagg
     3961 gagaggtact aggcttctcg ctcgtctata gcggaaactt tttggcgcag gtggatgtga
     4021 gttctttcca aaaaacaaga gtgagtatgg gaatccatcc tgagcgtttt tcttggacat
     4081 tagataaggg agaagagttc cagacgcctg aggttgttat ggtttatagc gagcatggtt
     4141 tgaatggcat gagccaaacc tatcaccgcc tcttccaaaa acatctggta cgtggctatt
     4201 ggcgtgatag agagcgacct gttctcttga ataactggga agccatgagt tttgattttg
     4261 atgaggaaac aattctttcc ttggccaaag aggctgcagg tcttggtgtt gaactctttg
     4321 tcatggatga tggttggttt ggcaagcgaa accatgaccg agcaggcttg ggagattgga
     4381 cggtcaaccg agagaaatta ccaagtggct tgacagggat tattgaccaa gttcatgcta
     4441 tgggaatgaa atttggactt tggattgaac ctgaaatggt taataaagac agcgatttat
     4501 accgagcgca tccagactgg attttccatc atcctcagca tagtcaatct cacggacgcc
     4561 accaatatac gttggatttg tcaagggagg atgtttatca aaatattcat gaccaactcc
     4621 atcggctgtt ggctgagcat gatattgatt atatcaagtg ggacatgaac cgctatatga
     4681 cagaggtatt tacctgcaca agggaggcgg agcggcaagg ggagaccttc catcgctata
     4741 ttctcaatct ctaccgttta tatgatagcc tgatagcggc ttttccgacg gttctgtttg
     4801 aatcttgttc aagtggtgga gctagatttg acccgggaat gctctactat gcaccgcaaa
     4861 cctgggtcag tgatgatacg gacgccatgg aacgcttgaa aatccaatat ggaacgagca
     4921 tggtatatcc tctaaatagc acgggttgtc atgtgtcagc tagtccgaat cagcagttgg
     4981 ggcgtgctac tccactaagc acacgtgcca atgttgcatt ctttggaagt tttggctatg
     5041 agttggattt gtttgagtgt agccaagaag agttggaaga gatgaaggag cagattgcct
     5101 tttacaaggc tcaccgtaag cttttccaac agggtatttt tactcggctg aaaagtccat
     5161 tcgatggaga aataacggct tggcaggtcc aatccttgga tggtcagcaa gtcatcgtgg
     5221 gttattaccg tcggttaaca acggctaatc tctcctatca acgcttgtat ttgcagggat
     5281 tggaagagga agcggtctat caccttgatg gagaagttta tactggtagc caattaatgc
     5341 atacaggttt gtcgattcga catggagacc atgttggaga caataaggac tttacttctt
     5401 atttgatggt ccttcagaaa caacagtagc atccctatcc ctaggcccag ctatcggctg
     5461 ggtctatttt atgcagaaag tacatcaatg agggcaagac ctctacttat actcttcgaa
     5521 aatcaaaatt atccgttgtc aacttgcctt gatgaactcc agttctatct tcggcttcgt
     5581 ttcctaggct acttttgatt ttcattgagt attagatata agaattcttg aaaaacgggt
     5641 tgcaatgtga gaaaatgaga gaataattct tcaatcaatt agtgagagga agggagataa
     5701 tgaatgaaga aatatgcttt tcgtgaaggg atgcgcgatg ccattcccac tgtattgggc
     5761 tatgccagta ttggtctagc ctgcggagtc gtatcggtta attcgggcat ttctactgtg
     5821 gagatggggc tgatgagcct attaatctat gccgggagtg ctcaatttgt tatgtgtgct
     5881 atgatttttg caggtgcgcc tttgttatcc attgcagtaa ccgttttctt tgtcaacctg
     5941 cgaaattttc tgatgtgctt gcatgcgaca actatttttc aaggaaatcg tttagggtcg
     6001 aatatcttga taggatcctt tgtgacggat gaatcctatg cagttctttt gcgtaagcag
     6061 attgagcatc ataccattac tccagaatgg atgtacggaa ataattttgc aagctacgcc
     6121 tcttgggtta cattcacgac tctcggcaat ctgattggtg gtctcattcc aaatcctgag
     6181 cagtttggat tggactttgc cttggttgcc atgtttgtcg gtatttttgc aggtcagtta
     6241 gaggctatgg ttcggaccat tccgctgaaa aaaattggct taattctgct ggcagtcggt
     6301 ctgacctata tcggtctgtc tatgatggtg tcgtcctatg tggcggtgct cttggcgaca
     6361 ctattaggtt gctttgtggg ggtgatgatt gatgataaaa actaacattt tactgattat
     6421 tctaatggcg gccctggtga cctgggtgcc acgcgtcctg ccttttgtcc tgacgcaaaa
     6481 taaatctctc cctccaaagt tggtgaaatt cctcagcttt ttgccaatca ctatcatttt
     6541 tgcattgacc ctctccagta ttatggatga gaaagttggc tccttgccaa gttttctccc
     6601 tgtggaaagc ttagctattc ttccgacatt ttttgtggtt ttgaaaacaa aaaatatcct
     6661 tttagcagtc ttggttggcg ttgttacaac cgcgttgtta cgtttgatat tttaggaggt
     6721 ttttatggta aaagaaaatg ccaaaggtgt cggactttgt ttccttttgg ctttggtggg
     6781 tcagtggtta ggtcatttat ttccacttgt aggtgggcct gtttttgccc ttctaattgg
     6841 gatgagcctg tatccctatt tttcaagtaa aaatgcattt cagtcaggct taacctttac
     6901 ttctaagaaa atcttacagt atgctgtgat ttgtttggga tttggattaa acttatcagc
     6961 ggtcttggca gttgggcgtc aatctcttcc aattattttt tcgaccatta gttttgcttt
     7021 gattttggca ttcctgatgt ggaaaatctt gcccatttca tctcatctgg caaccttgat
     7081 tggcgtaggt acctctatct gtggaggctc tgccattgcg gcgactgctc ccattattca
     7141 ggcagatgat gaggatgtgg cacaagcaat ctcggtcatc tttctcttta atgtcttggc
     7201 agccttggtc tttcctactt tggcaacttg gcttgggttt tcgactgatt cgggtcaggt
     7261 ctttgggatg ttcgctggaa cggcggtcaa tgatacctcc tctgtgacag cagcggcttc
     7321 gacctgggat agcttgtatg gtttaggtag tcagacctta gatacagcag tgacggttaa
     7381 gttaacacga actttagcta ttatcccgat tacaacggtc ttagcaattt ggcaaacccg
     7441 agggaaggga gttcaagcag acaagaagtc cctcttggca ggttttccgg tctttatctt
     7501 gtattttatc ttagctagtc taatcacaac gatagcgggt ggatttggta taggggcaga
     7561 ttggtttgct ccgctgaagt ccctctctaa agttttcatt tgtatggcta tggtggcgat
     7621 tggtttgcgg acaaatgtgt ttgccttggt gaaaaatggc agagcggctc tcctagtcgg
     7681 actagtttgc tggtttgggg taacagcgtt gacactggtt tggcaagcaa ttttggggat
     7741 ctggtaaata tagtctttta gtttactttt agtactcgct ccaactatct gggagatagt
     7801 tggagactgg ggataaagtg aacgatgttc acatcaaaag cagcagacat aactcaatgc
     7861 tttcttattt ctaggcattg acttgaaaca gtcccccaga ctgtttcaat caggcaaggc
     7921 gagttcaaac actccagtgg agtgtttgaa gttggaaata aggaaacaaa gtttcctcat
     7981 tcgtcgaagt aataaaagag aaactaaatg acgagaaaac agccttctct tgcgagaggc
     8041 tattttttat gatttcaagc ggaaagtctg cggagctttt ttcaacaaaa tccagtagcc
     8101 gatgctgaca taggtaatca tagctatgga aatgagtagg atgtacatgt gactgcctaa
     8161 atccagcttg atattgagat aggccaccca gtagagagcc ccactgagaa atttatagac
     8221 tgggttgaca acctccatgt ccttggtaaa gggttggaga atatagtaga taaagaggtc
     8281 gtggaaggaa aatagggctg ttagactcag caggagcaga gcagtcagga gataggtttc
     8341 aataggtagc tgcgctctag taaagatgat gagggccaat agtagagatc cagagaaggc
     8401 agcatttaat tttacagttt gcagaaaacg gtaatgaaag cctgcaataa tggtttttgc
     8461 ttcgcggtaa aatggatagt ggagcatgga aatatcgcaa ttaacaaaga ccatctgtgc
     8521 gacagcctta cccatggagg cggcatacat gaccatgaaa gcaaaaggca gtccctcgat
     8581 atagttggta ttgcttattt gaatgtcatc ttttgaaaac caacgaattc cttcaataaa
     8641 aaggacaata gccaaaagac aacccaagcg aaagtgcagt tttttcgtaa ggacagaacg
     8701 atagcggtca aatagcagag cgtttaagta ggtcatgcct gacagatgag tcaggtcctt
     8761 tcctgtttcc atactcagtt tcgcctgcat ctgtaaacct tgtcgggtgt actcgttccc
     8821 tttggtcatc tcaaaaattt tcttatccat ctgcaaggac tggtccatgc aataatagag
     8881 atagtctaga tggttggttt gttgcttcaa ataaccatag ccaaaccaaa tgagcaagac
     8941 ctgacagaca agcacaggca gtaaaaagat gggcgacaga tgattgcgga aaaggataag
     9001 gcaggcaatt gctgctagac cagtacccaa gataacccag gattgccaag aattacggcg
     9061 ggatagaatc cgtcgattaa aaaaccaacg attgagagcc tgtccggtaa aacttcctgt
     9121 cggaatggtc agtaatccca gaggcagata gagccaattt cctgataggc cagaaaaaat
     9181 cagcagggca ggcaggtaga acagactggt gataataggt tctacaaata gttgactctg
     9241 gagaaaacta gactgggaaa gtccaaaatt ttgcatgaat tcccgctcgg acttgacgat
     9301 aaagggctca aaacccttgc taaaccgata ggtgtagcca acaaagacca tccagagaga
     9361 aaagcccaaa agccaagtcc ctggtagaat attccaaaaa gtcaaatccc cgccagagag
     9421 gagattgatc caaaaggatg ccaagtagaa attcagggct aaccaaattc ccttgaccag
     9481 aaagcgactg gggatggaga ggacgtgcag gatcaaaaag agaccagact taaaactgta
     9541 agacttgaaa atactctctg gaatgctctt gcccacaagt ggcagttttc gcagatagta
     9601 gaaaaatccg ttgacggtcc gatagaggtt atacttttcc tggtaccaaa aataacgtag
     9661 aatggttttc ataagtccct cctagtctgc tgtcaagagg gtaaccactt cctgttcaaa
     9721 atcagggtcg tggagcttgt cactcggaac agcctgtagt tcttggtgat gtagtaaaac
     9781 aatctcgtca cagagatcct gagccagttg tagaatatgg gtggaaaaga tgataacaga
     9841 atccttttta gcctcccgaa tcagctgttt gaactcgtgg gcagccacag ggtcaaagga
     9901 agtcagcggc tcatctaaga gaaggacagg tggctggaca atcaaagaca gaagcaactg
     9961 gaccttgttc tgcatcccgt gggaaaagtc cttgagtaga cggtgccggt cttcttctcc
    10021 gataccgacc aagtttagcc attcttctgg actacgagga gatttgagtt tatccttgtg
    10081 gatatccata tagaaacgga caaactcgta agccgtcata aaggcaggta gttgcggata
    10141 ggtctgggta aaaccgatgt ccgtattatc gtagtcaaag gcaatttcat ccttcacaaa
    10201 ctgaatcgag ccaccatcca aggtcagatt acgggcaatg cagttgaaca aggtcgtctt
    10261 accagcccca ttccgcccta gcaaaccgta aatcttgcct tgttcgaagg taaaggaagt
    10321 atcttccaaa atgattttat ggtcgaattt cttggaaaca gaagataatt tcagttgcat
    10381 agccaatctc cttttgttat attgttatgg ttttatcata caacaaaaaa caaaaaaatt
    10441 caagcatatt ttgcttgaat ttagaaagag gataggggtt gtttttgctg accctggcca
    10501 tcgaagttac tatcctttag ccagttttca agggcacgct tacggtcagg ccattcgtgg
    10561 tctaatagag aaaaccaggc agtatcccga ttgcgttcct tatagactag ggcttgacgg
    10621 aagttgcctt cgtaggtaaa gcccagacgg ttggcagcga ctcgactggc ttggttgagc
    10681 gagtcgcatt tccattcgta gcgtcgatag ccaagttctt caaatacata ggtcatgaga
    10741 agatagtgag cttctgtcgc ctgtcgggtc cgttgcagtt gcggagaaaa gatgacccag
    10801 ccaacttcaa tggtacgatg gttggtatct attcgcatga gtgcaaaggt tccgactgcc
    10861 tgattggttg cttggtcaag gatggcaaaa taataggggt cggtcgagtt caaaagagtc
    10921 tggaaatagt cctgaaatgt cgctctgtcc tcaaagggtt caaggggcaa gtaggtccag
    10981 tcagttttct tggcacttgg tccataaaac tgatagagat catctgcatg gtgcaggctc
    11041 agtttttcta agcggactgt gtgtccttgt atagtctcca gacttggtcg ctcaccgcta
    11101 ctataatgcg gtaagggcat accaatcttt tggccgaatg aattgatagg catggtttct
    11161 cctttaggtt gagcttgaag agcgatttat aaaagtgcaa tactcaaaga aaatcgaact
    11221 cagactacct ccacaggttc ggggaacctt tggaggttgg aaataaggcg aacaaagttc
    11281 gtgccattag atgtagatag aacgctgcta ccttttattt tc
//
LOCUS       DASFSW010000068        11219 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.068, whole genome shotgun sequence.
ACCESSION   DASFSW010000068 DASFSW010000000
VERSION     DASFSW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.068"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(324..986)
                     /locus_tag="U1099_001284"
     CDS             complement(324..986)
                     /locus_tag="U1099_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM4867603.1"
                     /translation="MTKYYINKFKNQPLVRIGYARVSSSDNRQELGLEVQKEALKDCD
                     IIFIEKDSGGKIDRPRLNKAMKVAKALADSGTQVVLVVYKLDRLTRKMFQLSDIIQEL
                     TSHSIQLKSLQENIETNSLTGKFFCLILGYVAEWELESIRNRTKDGLRKARERGVKLG
                     NKGIPKELENRIIRSYRLTDTPIRELSKNLNISTATIYSVLKRNNIQTNRKFNPKIVD
                     KN"
     gene            complement(1105..1500)
                     /locus_tag="U1099_001285"
     CDS             complement(1105..1500)
                     /locus_tag="U1099_001285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867604.1"
                     /translation="MLRFRNKRIQTVSNNEMIHYYSLEKHKINALHSLVDFAENLSAK
                     GFKLDSSFQSAYDACLVMYQRTGVPSFSYLERLYGEIIPIYYNNKWQGHIMAESPKKL
                     ELALHWVGQMNANADELQSALIDLKMIES"
     gene            complement(1708..2433)
                     /locus_tag="U1099_001286"
     CDS             complement(1708..2433)
                     /locus_tag="U1099_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4867605.1"
                     /translation="MSSFIREIKVENLPNNEYYSHIPAIKSLDKLKLSAPVTFFVGEN
                     GSGKSTLLEAIAVSLGFNAEGGTINFNFSTKETHSSLYRHIKIIKGHKRPQNNFFLRA
                     ESFYNVASYLEEIYRREIFNGYKLDNYGGIPHEHSHGQSFLGLVLNKFQPNGLYLLDE
                     PEAALSPQNQLTFMARIHELSKAGCQFIIATHSPIIMSLPNSTVFEFKSTIEEINYKD
                     SESYQITKLMLEDSERVFRNLLE"
     gene            complement(2489..3595)
                     /locus_tag="U1099_001287"
     CDS             complement(2489..3595)
                     /locus_tag="U1099_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867606.1"
                     /translation="MTRFRLGWFCYILQKCEKKKRNQEDFVNTVLNKLVGKGNPKTSF
                     IPEINRVSSNRLFTGEENVPEFYLEALPLTDYDFQTKRISSVLESINENEDFNYKQAV
                     IKAKNELSMYDKDAIITFGSAKLEALKNAPTNELFIASLLIYLVNTPNIPQKDTDELR
                     HSKIIQDNILKFLENEILLTPFSHRTTFEGVLANPIINQSYFIECYTTEALNNPELIG
                     TLMTSSSLRLLTRDSIIKMPIIQNGFIIQNGSINERTQKFISEKFQTAPKVTLLMGLY
                     QNKDEAFKAIDRYFPKEAIGKIFTIESMAVSIKSSYDYTAQKMKDMIHLTNDAVEYLE
                     NRNFADKDSIELTVGGVEQLNGGECLVYLLTKCI"
     gene            3711..3980
                     /locus_tag="U1099_001288"
     CDS             3711..3980
                     /locus_tag="U1099_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867607.1"
                     /translation="MSLIPSIRRNKEVQALQTIASPYQMEVARKLSQNMADNQARELL
                     ATDILYKVGNLALIQAEILKNNPEAHAYTDYILRAFTHYTTQYLK"
     gene            3991..4203
                     /locus_tag="U1099_001289"
     CDS             3991..4203
                     /locus_tag="U1099_001289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867608.1"
                     /translation="MNIYSWILIISGVILLFISALGAYFEALDKENQPRYTEEEITLA
                     EYLEKKQAINGIYLEAVAEILRHQKR"
     gene            4361..4531
                     /locus_tag="U1099_001290"
     CDS             4361..4531
                     /locus_tag="U1099_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867609.1"
                     /translation="MKKLLAYILGFFACAWLVNQAVKLVLEVWQVLFLGAVFIFAGWF
                     AFRIIKIKKDWR"
     gene            4534..6738
                     /locus_tag="U1099_001291"
     CDS             4534..6738
                     /locus_tag="U1099_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEM4867610.1"
                     /translation="MKRHFNALSWSEWTWQRPFSEEDVKSLLGQLVGLARRKSLVFEV
                     RLKKNRVRYLLGTEEQDKRYIHQLIQSHRQIQFSKAPKREKISVARLVQIKQSHYALK
                     TDSLENMIRSSLALSKTLQPDEEIVLQLILGSGSPPRPQPKELPNLSARWYQVITNNI
                     PELSENSKKLMRQKLNQSTFRCEIRLGANSRSILRTKEFFSSLLSCFRMLETHATIEL
                     KPISASKINQAQPSWSFPYSLATTDLACFLLLPVGEENMDGVPNVHPKLITPPLGYNL
                     NHNKARSFGQTIETQPRPLQISSQSAKKHLFLLGSTGTGKTTAMSHLILSDIKSKKHS
                     VVVIDAKGQLTHELLERTPAEHDEDIVVISPTAKRIVGINPFELTNYGIEPEVIADYL
                     LELFKGLYPEHFGIYSLDILSHAFLTLARIPNSSLVMLPSLLTNKHFRNKLLKNLTDP
                     IGLESFWSWFEMLSEAQRHQMLNPILNKFRQFLLRPQLRAMLGQAQPKFSLAELFQGR
                     KIVLVPLNKAVIGSESAKLIGSLVTSILWMLILRQSSVEPSKRQSAFIYVDETPSFLG
                     IPNANLDEALSQSRQFNVGWAIGFQHLAQMSSNLKAGIESNTANKIVFGLNLNEAKEM
                     AKSTLEVAKEDFYSLPPFWVYSRIETSPNTYRWVIGKTYPPTPRIRDSRTPYLNSLIR
                     YGQNVDDLEREFQAFTYQEKPQTAESLDNLGRKKRSNRSSNRVDEEDSSSPA"
     gene            6826..7623
                     /locus_tag="U1099_001292"
     CDS             6826..7623
                     /locus_tag="U1099_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEM4867611.1"
                     /translation="MKWNQLTSQDIQILYFLNQARYASTSQLARLFYSDSDKPETAIR
                     RANFTTQRLLKAGLVSHLKRRIGGVRKGSASYVWQITFQGLKLLKSQDETIVLRYKNH
                     YEPSQHHVEHTLAVTEIFIETMETVRDSQKLSLEAFSFEPNSWRSYQKLSGLGMTLKP
                     DAYLELVSQEYEDHYFIELDRSTESLARIVNTCKKYIEYYRSGVEQRQHGVFPFVLWI
                     VPDDKRKLAISKTIQKELYNFWELFTVITLDDYQNYIKGGLNNERKE"
     gene            7858..9492
                     /locus_tag="U1099_001293"
     CDS             7858..9492
                     /locus_tag="U1099_001293"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012464.1,HMM:NF019149.1,HMM:NF024800.1,
                     HMM:NF025645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM4867612.1"
                     /translation="MKSTTLQRTLTTKYSSLDRAEELTALYCRISNEDMNAGDSNSIL
                     NQKSILEKYAKDNRLGTYRFFIDDGYTGTNFSKRPAFQEMLELVNAGIVKTVVVKDLS
                     RFGRNATEVGLYTDILFPKLDVRFIAINDNVDSANSNITDLDYSGFLNIFNEFHSKST
                     SAKVRSVLRSKAERGERIGGFAPFGYQLDPKDKNHLVIDEVAAEIVRRIFRLYLDGMT
                     ISKIANLLSSEKVPTVTKYRQKVVDPNCDWYRESVKNILSRPEYCGDLVLAKSYIKSY
                     KDKRKILNDAKDWLVFKDRHEAIIPRDQFELVQTMLAKKRRRTKSNRHGLFSGLVYCH
                     DCGYRHYFCTGKSISPNQERYICSGFQSKKVKCSDSHYIRELKLQEIVLSDLNDKIQY
                     VKKHKQEFLQLLVSKTEKDSKKELKATERRLSECQKRTSQLDIIIKKLYEDNVLGKLS
                     DERFMSLSKDYEHEQNLLKSEILEKSSYLSEQKDKLMDVNRFISIIDQYTTLTELNSS
                     IVNELIERIEIHQPDKSSGKRVQQIDIFYTFVGKFN"
     gene            complement(9574..10908)
                     /locus_tag="U1099_001294"
     CDS             complement(9574..10908)
                     /locus_tag="U1099_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEM4867613.1"
                     /translation="MLELFKAINNLVWGPPLLLLLVGTGVYLTLRLGVFQIGKLPTAF
                     RLIFSSDQSGQGDVSSFAALCTALAATVGTGNIVGVATAITTGGPGALFWMWVAAFFG
                     MATKYAEGFLAIKYRTKDANGQAAGGPMHYITLGMGQKWKPLAVFFAISGVLVALLGI
                     GTFSQVNSITASLETSFGLEPPLVSVITAISIAFVVFGGIEKISDVSTKIVPFMAILY
                     ILASITVLAVHWDQLLPTLALVFKSAFTPAAAVGGFAGATVQQAIQRGIARGVFSNES
                     GLGSAPIAAAAAKSDNPVEQGLISMTGTFIDTLIICTLTGFTILVTDQWSLEGLAGAP
                     LTQAAFATVFGNTGSIALTISLVLFAFTTILGWSYYGERCIEFLFGTKAILPYRLVFV
                     AMVALGGFLKLDLIWTIADIVNGLMALPNLIALLALSPVIIKETRQYFAKKK"
     regulatory      complement(11014..11118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT         3537 a         2279 c         2105 g         3298 t
ORIGIN      
        1 caataacagc taccacaaga aaaattatta tttccacaca tttctccttt ctgtataaaa
       61 ccgcctatct gatagataag cgcctaaaag acaacttaaa actccttcct gctttcttca
      121 aaatccctgc tacaatagga tttgagtgtg ttttagtgaa cggtcgttaa ctaaaacaat
      181 gaaaaatcaa aaaatgccga cttggcaagg cttcaaaaaa accgtcaaat cagcatttca
      241 aatatttttt attgataact ttgattgttt taacaaacga ccgtctgcta aaacattttt
      301 acgagtttaa tttactattt tttctagttt ttgtcaacga tttttgggtt aaattttctg
      361 tttgtttgga tattgttacg cttcaggacg ctataaatgg tagcggtaga aatattcagg
      421 tttttggata attcacggat aggcgtatca gttaaacgat aactccgaat tatccgattc
      481 tccaactctt ttggaattcc tttattacca agttttacgc ctctttctcg tgcttttctg
      541 agtccgtctt ttgttctgtt gcggattgat tccagttccc attcagcgac atagccaaga
      601 atgaggcaga agaactttcc agttagagaa ttagtttcga tattttcttg taagcttttt
      661 agctgaatag agtgagaggt taattcttga atgatgtcag atagctgaaa cattttccga
      721 gtaaggcggt ctagcttgta taccacaaga acgacttgag tgccactatc cgcaagagct
      781 ttagccactt tcatagcctt gttaagtctt ggtctgtcta tttttccgcc agaatctttt
      841 tcgatgaaga taatgtcgca atctttgaga gcttcctttt ggacttccaa acctaattct
      901 tgtctgttat cgctagagct aacacgagca tagccaatac gaactaaagg ctgatttttg
      961 aatttgttga tatagtattt tgtcacgatt tctcctttac ttaaatttga tattgtagca
     1021 gggaggcgag tgacaaaata gactataaat aaaaagtata aagctactat ctgaacactt
     1081 gtagctctat acttttgttc tgtttcatga ttcaatcatt ttcaagtcaa ttaatgcaga
     1141 ctgtagctca tcagcattcg cattcatttg accaacccaa tgcagagcta attctaattt
     1201 tttcggagac tccgccatga tatgcccttg ccatttattg ttgtagtaaa ttggaattat
     1261 ttctccgtac aaacgctcta aataagaaaa tgacggcaca cctgttctct gatacatgac
     1321 taagcacgca tcatacgcag actggaacga gctatctagc ttaaaacctt tagcagaaag
     1381 attctcggca aaatcaacta aactatgtaa agcattaatt ttatgcttct ctaatgaata
     1441 gtaatgaatc atttcattat ttgaaaccgt ctggattctt ttatttcgaa atcttaacat
     1501 ttaaccaccc cttagcaaaa ttagcaataa gcacatttcc cagttccaga gcactctata
     1561 caacggcttc cgccagacgg atagacgtta taagggcttg gatgggggtt gtttccacgg
     1621 tcgtctcctg ttccattgca gaatgaacag attttggtat cgttacattc tctacattta
     1681 gccatatcat atgacctcct ttttttatta ttctaaaaga ttacgaaata ctcgttcgga
     1741 atcttccaac attaactttg tgatttggta actttccgaa tctttatagt taatttcctc
     1801 aatagtagat ttaaattcaa acactgtaga gttaggcaaa gacatgatta ttggggaatg
     1861 cgttgcgatt atgaactgac aaccagcttt gctcaactca tggattctag ccataaaggt
     1921 tagctgattt tgcggagaaa gtgcagcctc cggctcatca agtagataaa gcccattcgg
     1981 ctgaaatttg tttagaacta gtccgagaaa tgactgaccg tgagagtgtt catgaggaat
     2041 ccctccatag ttatcaagct tatacccatt aaaaatttcc ctgcgataga tttcctctaa
     2101 gtaactagca acattataaa aactttcagc tctaaggaaa aaattatttt gaggtctttt
     2161 atggccttta attattttga tatgtcggta gagtgaggag tgtgtttctt tagttgaaaa
     2221 gttgaaatta atcgtcccgc cttctgcgtt aaatcctaaa cttaccgcta tcgcttcaag
     2281 cagtgtcgat tttccactac cgttctctcc aacgaagaag gtaactggcg cagataactt
     2341 taatttatcc agacttttta ttgctggtat atgagaatag tattcattat ttggtaaatt
     2401 ttcaactttt atttctctaa taaaactact catgaaaagc tcctttacaa ttagtatttt
     2461 agcaaatagc ctctattttg taaaacaatt atatgcattt tgtcaataaa tacactagac
     2521 actctccgcc gtttagctgc tcaacacccc caaccgtcaa ctcgatacta tccttatccg
     2581 caaagttccg attttcaaga tactccactg catcatttgt cagatgtatc atatctttca
     2641 tcttttgggc ggtatagtcg taactagatt ttatagagac cgccatactt tctatcgtga
     2701 atatcttgcc aattgcttct ttggggaaat agcggtctat tgctttgaat gcttcatctt
     2761 tgttttgata taagcccata agcaaagtaa cttttggggc tgtttggaat ttttcagaaa
     2821 taaacttctg agttcgttca ttaatacttc cgttttgaat aatgaagccg ttctgaataa
     2881 ttggcatttt tataattgag tctctagtaa gcagacggag agacgaagaa gtcattaagg
     2941 tgccaattag ctctggatta tttaatgctt ctgttgtata gcattctata aaatagcttt
     3001 ggttaattat aggattagct aatacacctt caaaggtagt tctatgcgag aacggagtaa
     3061 gtagaatttc attctctaaa aacttcaaga tattgtcttg aatgatttta gaatgtctca
     3121 attcatcggt gtctttttgg gggatattgg gggtgttcac tagatagata agtagactcg
     3181 caataaataa ctcatttgtg ggagcgttct ttagagcttc aagtttggca gatccgaaag
     3241 tgataatagc gtctttatca tacatagaaa gctcattttt agcctttatt accgcctgtt
     3301 tatagttaaa atcttcattt tcatttattg attcaagaac agaagaaatt ctcttagttt
     3361 gaaaatcgta gtctgttaat ggaagggctt ctaggtaaaa ctcaggtaca ttctcctcgc
     3421 cagtaaaaag tctattacta gaaaccctat ttatttctgg gatgaaagag gtcttgggat
     3481 tacctttgcc tactaattta tttaatactg tattgacaaa atcttcttga tttcgttttt
     3541 tcttttcaca tttttgaaga atataacaaa accaacctaa tctaaacctt gtcatctgct
     3601 cctccttgag atttttgaga ttttttgact gatgaatgag atttaataat caattatact
     3661 ttaaccataa gttagctaac ttaccaaaat tatatcaaag gagaacgctt atgtcactta
     3721 taccttctat tcgtcgaaac aaagaagtcc aagcacttca aaccatcgcc agcccatatc
     3781 aaatggaggt cgcaagaaag ctcagccaaa acatggcaga caaccaagca agggagcttc
     3841 tggcgacaga cattctctac aaagtgggga atctagcctt gattcaagct gaaatcctga
     3901 aaaacaatcc agaagctcac gcttatacag attatattct gagagctttc actcactata
     3961 ctacgcaata tttgaaataa ggaggtgttt atgaacattt attcttggat tctgataata
     4021 agtggcgtta tcttactctt tatttctgct ctaggggcat actttgaagc attagacaaa
     4081 gaaaatcagc caagatatac ggaagaagaa atcacattgg cagaatatct tgaaaagaaa
     4141 caggcaatta acggaattta cctagaagct gtcgccgaaa tcttacgcca ccaaaagcga
     4201 tagaatcacc acttggatta gacaaaagga caaaatatgg tgaaatatac tactgttaag
     4261 tcgtccaaaa tctattccaa tcgctccaaa agcaggacaa agccaaattt caatttttgg
     4321 caatcttcct gtataactga aatcagaaag gaaaccagcc atgaagaaac tactcgctta
     4381 cattctcgga ttctttgctt gtgcttggct tgtcaatcaa gcggttaaat tggtcttgga
     4441 agtctggcaa gtgttgtttt tgggagcggt gttcatcttt gctggttggt ttgcctttcg
     4501 gattatcaaa attaagaaag attggaggta aatatgaagc gacactttaa cgccttgtcg
     4561 tggagtgaat ggacttggca acgtccgttt tcagaagaag atgtcaaaag tctgcttggt
     4621 cagttggtcg gtctggctcg gcgaaagagt cttgtttttg aagttcgctt gaagaaaaac
     4681 cgtgtgcggt atcttttagg cacagaagaa caagataaac ggtacattca tcaattgata
     4741 caatcacacc gacagattca attttctaaa gctcctaagc gtgaaaaaat atcggttgcc
     4801 cgattagttc agattaagca gtcacattat gctctaaaaa cagattcgtt agagaatatg
     4861 attcgctcta gtttggcact gtcaaaaaca ctccaaccag atgaggaaat tgttcttcag
     4921 ttgattcttg gctctggctc tccaccaagg ccacaaccta aagaattacc gaatttgtct
     4981 gctcgctggt atcaggttat cacaaataat attcctgaac tctcggaaaa ctccaaaaag
     5041 ctcatgcgtc agaagttaaa tcaatcaact ttcagatgtg aaattaggct tggggcaaat
     5101 tcacgcagta ttttgcggac gaaagagttc ttttcaagcc tgctcagttg ctttcggatg
     5161 ttggagactc acgccactat cgaactaaaa ccaatctcgg cttcaaaaat caatcaagcc
     5221 caaccatctt ggtctttccc atatagccta gctactactg acctcgcttg tttcctgctt
     5281 ttgccagttg gtgaagaaaa catggacggc gtgccgaatg ttcacccgaa actcatcacg
     5341 ccaccgcttg gctacaattt gaatcacaac aaggcgagaa gttttgggca gactatcgaa
     5401 actcagccga gaccgcttca aatttccagc caatccgcca agaaacattt gtttttactt
     5461 ggaagcactg gaacaggtaa aactacagcc atgagtcatt tgattctgtc tgacataaaa
     5521 tccaagaagc atagcgtggt cgtgattgac gccaaggggc aattaacgca tgaactgctt
     5581 gaacgaactc cagccgaaca tgatgaagac atagtggtta tttcaccaac cgccaagcga
     5641 attgttggca ttaacccctt tgagctgaca aactatggaa tcgagcctga agttatcgca
     5701 gattatctgt tagagctatt caaaggctta tatccagaac atttcggaat ctactctttg
     5761 gatattctgt cgcacgcttt cttgactttg gcacggattc caaacagttc tttggtgatg
     5821 ttacctagcc ttttaaccaa caagcatttc agaaataaac tgctgaaaaa tctcacagac
     5881 ccaatcggct tagaaagttt ttggtcttgg tttgagatgt taagcgaagc ccaacgccac
     5941 cagatgttaa acccgatttt gaacaagttt cgccagtttt tgcttcgccc gcaactcagg
     6001 gctatgcttg gacaagctca gccaaaattc agtttggcag aactcttcca gggcagaaaa
     6061 attgtgctag tgccattaaa caaggcggtt attggttcag aatccgccaa acttattgga
     6121 agccttgtca cttcaatctt gtggatgctt attttgcgac agtcttcggt tgagccaagt
     6181 aaacgccagt ccgctttcat ttatgtcgat gaaacaccga gctttcttgg aatccctaac
     6241 gccaatctgg acgaagcact ttcgcaatct cgccagttta atgttggctg ggcgataggc
     6301 tttcagcact tggcacaaat gtcttcaaac ctcaaagcag gtattgaatc taatactgcc
     6361 aataaaattg tctttggctt gaatttgaac gaagccaagg aaatggcaaa atctacatta
     6421 gaagttgcca aagaggattt ttatagcctg ccaccatttt gggtatattc aagaattgaa
     6481 acttcgccca atacctatcg ttgggtaatt ggcaaaacct atccgccaac gcctaggatt
     6541 cgggatagtc gcacacctta cctaaatagt cttatcagat acggacaaaa tgtggacgat
     6601 ttagagcgag aatttcaggc ttttacctac caagaaaaac cacaaacggc tgaatctctg
     6661 gacaatttag gacgaaaaaa gcgttctaat cggtcttcca atcgtgttga tgaagaagat
     6721 tcttccagcc ccgcgtaaaa taagcctttc tgccctcaat tgtcacacga cttactgcgt
     6781 aacgaagcac attttaccag ccaaccttta aggagaacaa aacagatgaa atggaatcaa
     6841 ttaacctctc aagatattca aattttatac tttctcaatc aagctagata tgcctccacc
     6901 agccaactcg ccagactctt ttattcagac agcgacaaac cagaaaccgc tattcgccga
     6961 gccaacttta caacgcaaag actgttaaag gctggtctgg tttcacacct caaacgccga
     7021 attggtggtg ttcgcaaagg ctctgcttcc tatgtttggc aaattacttt tcaaggtttg
     7081 aaactattaa aaagccaaga tgaaacgatt gtcttgcgtt ataaaaacca ctacgaaccc
     7141 agccaacacc atgtcgaaca taccttggcg gtcacggaaa tattcattga aactatggag
     7201 accgtccgag attcccaaaa gctatcactt gaagcctttt ctttcgagcc gaactcttgg
     7261 cgaagctacc aaaaactttc tggcttaggt atgactttga aaccagatgc ttatttggag
     7321 ttagtcagcc aagaatacga agaccactat tttattgaac tagaccgaag cactgaaagc
     7381 ctagcccgta tagtgaacac ttgtaaaaag tatatcgaat actatcgctc aggcgtcgaa
     7441 caacgccagc acggagtttt cccatttgtg ctttggattg ttccagatga caaacgaaag
     7501 ctagccattt ccaaaactat ccagaaagaa ctttataact tttgggaatt gttcaccgtg
     7561 attactctgg atgactacca aaactatatc aaaggaggat taaataatga gcgaaaagaa
     7621 taaagcctct aaggttataa cggacggtaa aaagacatac attattaact ctaaatcaag
     7681 tttgctcgca agtaaaaact tagaaagagt cctaattcgt ttgatgttga gagagttaga
     7741 aaaaagccga aaaccatagt ttttccaacg gttatgccta caaattaaac cttgaaaatg
     7801 gtataatata ggtatagccg taaggttttt atagctttct caaaaggaga aaatgttatg
     7861 aaatctacta ccttacaacg aactttgaca actaaatatt catctttaga tagagccgaa
     7921 gaactgactg ctttatactg ccgaatttct aatgaagaca tgaacgctgg agatagtaat
     7981 tcgattctga atcagaaatc aattttggaa aaatacgcca aagataaccg attgggaaca
     8041 tatcgcttct tcattgatga cggttacacg ggaacaaact tttccaagcg accagccttt
     8101 caagaaatgt tagagttggt taatgctgga attgttaaga cagtagtcgt caaagatttg
     8161 tcgcgatttg gacggaatgc gaccgaagtt ggtttatata ctgatattct atttcctaaa
     8221 ttggatgttc gttttattgc gataaatgat aacgttgatt cagctaactc taatataacc
     8281 gaccttgatt attctggatt cttgaatatt ttcaatgaat ttcatagcaa atcaaccagc
     8341 gctaaagtgc gctcagtctt gcgaagcaaa gccgaacgag gtgagcggat tggaggattt
     8401 gcgccatttg gttatcaatt agacccaaaa gataaaaatc atctcgttat agatgaagtg
     8461 gctgcggaga ttgtgagacg aattttccga ttatatctgg acggaatgac gatttctaaa
     8521 atagctaatt tgttatccag tgagaaagtc cctaccgtaa caaaatatcg acaaaaagta
     8581 gttgacccaa actgcgactg gtatcgtgaa agtgttaaaa atatactttc cagaccagaa
     8641 tactgcggtg atttggtgtt ggctaaaagt tatatcaaat cctataagga taaacgaaaa
     8701 atattgaacg atgctaaaga ttggctagtc ttcaaagata ggcatgaggc gattattcca
     8761 agagaccagt ttgagcttgt tcagacaatg cttgccaaaa agcgccgtcg gacaaagagt
     8821 aatcggcacg gcttattttc aggcttagtt tattgtcatg attgtggtta tcgtcactat
     8881 ttctgtactg gaaagagtat ttcacctaat caagaaagat atatctgttc tggatttcag
     8941 agcaaaaagg ttaaatgtag tgattctcat tatatccgag agctaaaact acaagaaatt
     9001 gtattaagcg accttaacga taaaattcaa tatgtaaaga agcataaaca agagttttta
     9061 cagttactag tatctaagac tgaaaaagat agtaaaaaag agcttaaagc aactgaacgc
     9121 cgattgtctg aatgtcaaaa acgaacgagt cagttagata ttattatcaa aaagctatat
     9181 gaagataatg tcttaggtaa gttgtctgat gaacgcttta tgtctctatc taaagattat
     9241 gaacatgagc agaatctcct aaaatcggag attctggaaa aatccagcta cctgtctgaa
     9301 caaaaagata agttaatgga tgtcaatcgc ttcatttcca ttattgatca atacaccact
     9361 ttaacagagt taaattcaag tattgtcaat gaactaatag aacgaattga aatccatcaa
     9421 ccagataaaa gtagcggaaa acgagtccaa caaattgata tattttatac ctttgttggc
     9481 aaattcaatt aaaaaccaga caggattcct gtctggttac aatataccct tttggtaggt
     9541 tagatagttc tacctaagtg gctgactttt atcttatttc ttctttgcaa aatactggcg
     9601 tgtctccttg atgataacag gagagagagc tagtaaggcg atcaagtttg gtagggccat
     9661 gagaccattt acaatatcgg caatggtcca aatcaagtct agtttgagga aaccacccaa
     9721 ggcaaccata gctacaaaga ccagacggta aggtaagatg gctttggttc caaatagaaa
     9781 ctcaatacag cgttcaccat agtaagacca tccgagaata gttgtaaaag caaaaagtac
     9841 caaactaatc gtcaaggcaa tagatcctgt attgccaaaa acagtcgcaa aggctgcctg
     9901 cgtcagagga gcacctgcca agccctctag cgaccactgg tcagttacta gaatcgtaaa
     9961 acccgttaag gtacagatga taagtgtatc aataaaggta cccgtcatag aaatcaagcc
    10021 ctgttcaact ggattatcag acttggcagc cgcagcagca ataggcgccg aacccaaacc
    10081 agattcattt gagaaaaccc cacgcgcaat cccacgctga attgcttgtt gcacagtcgc
    10141 acctgcaaaa ccgcccacag ccgctgctgg agtaaaggca gatttgaaaa ctaaggccag
    10201 ggttggcaag agttgatccc aatgcacggc caagacagta atactagcta agatatacaa
    10261 aatcgccatg aaaggaacaa ttttggtcga aacgtcagag attttttcaa tcccaccaaa
    10321 aacaacgaag gcaattgaaa tagccgttat tacactgaca agtggcggtt ccaatccaaa
    10381 actcgtttcc aaagaagcag tgatggaatt aacttgcgaa aaagttccaa tccccaagag
    10441 agcaaccaaa acacctgaaa tagcaaagaa aactgccaag ggtttccact tctgtcccat
    10501 tcccaaggtg atataatgca taggtccacc agctgcttgc ccattggcat ccttggttct
    10561 atatttgatg gccaaaaagc cttccgcata cttggttgcc atcccgaaaa aggccgcaac
    10621 ccacatccag aaaagagccc caggaccacc tgtggtaata gctgtcgcaa ctccgacaat
    10681 atttcctgta ccaactgtcg ctgccaaagc tgtacaaagg gccgcaaagc tggacacatc
    10741 tccctgacca gactggtcac tggagaaaat cagacgaaaa gccgtcggca atttgccaat
    10801 ctgaaatact cccaaacgaa gagtgagata aacacccgtt ccgaccaata gtaacaagag
    10861 gggcggcccc caaacaagat tgttgatagc cttaaatagt tctaacatag ttcctcctaa
    10921 gcgtttctaa tccgataaag ctgccggaaa aacaaaaaga ggctgaacac ttcaacctct
    10981 agaatcaacg tgaaaatata gaaaaagcct atttttcaac gctctgtcct tttacctgag
    11041 agtttcagta gctctacttt tccaagcagg actactttgc cccttcggtg ccaaaatggt
    11101 ctctccagag ttccgtccat tgaacagtca gtttcccttc tacccaatat caaccggtta
    11161 ttcagtttta tctagtttta ttttaatcta atttcacgaa aaaatcaaga atattttct
//
LOCUS       DASFSW010000069        11004 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.069, whole genome shotgun sequence.
ACCESSION   DASFSW010000069 DASFSW010000000
VERSION     DASFSW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.069"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            27..698
                     /locus_tag="U1099_001295"
     CDS             27..698
                     /locus_tag="U1099_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin lysis protein"
                     /protein_id="HEM4867614.1"
                     /translation="MKTFISKLMLVSTVVLLAACQSNQVKGGESSSSSASSQVSDGSS
                     LQQASTIASSSETQASSVTTPELSEVYQSVIDRYQANMGQSAEAINQDEVSSYLSLLT
                     SQGQEYSGIFYSLYDVNHDGTEELLLALDKSGEYVLIDLYTQLADESLRLVDNFRNLG
                     FEIGPNALLRPLQDGTYLFEGDGIFRIYQYNAMIPGLKRISESDTNPETSPLLELKSL
                     HWVKL"
     gene            867..1934
                     /locus_tag="U1099_001296"
     CDS             867..1934
                     /locus_tag="U1099_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HEM4867615.1"
                     /translation="MKTNLKKLLYSGVTLMSLGVLAACSSTSSSTTTSSSAATSQTSV
                     ATTSNNSTSSDSSSSTIDWSALPTTEVTLSNDGLKITEGGTYILTGSTTAGVTVETDA
                     NVRIILAGAEISSSDTAAINVINADNVELEIKDGTTNTVKDTSNHTDTNIEGAIHVEA
                     DLTITGNGSLTVEGNFQDGIVSTDDLVVNSGNITVTAVDDGIRGKDSLTINGGEITVT
                     AGDDGIKAETALTIDGGTITVSESAEALEGTNITINGGTLDVYGSDDAINAASTTSSE
                     IFIKVTGGDLKVAVGSGDTDAFDANGDIYISGGTIDVTAQSAFDFDGTAELTGGTVTV
                     NGEQITQITATGPGAGGHGGW"
     gene            complement(1970..2629)
                     /locus_tag="U1099_001297"
     CDS             complement(1970..2629)
                     /locus_tag="U1099_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEM4867616.1"
                     /translation="MEFDLFLMICNYIGTIAFAVSGAVKGFKKKLDIFGISLLGIITA
                     VGGGIIRDTMANRIPAALTDPTAIYLSLGVSISMYLIVINLKQDKPLDKKMIHFLSQT
                     NLIFDAIGLAIFALIGASTGISLQLNALTSGILAALTGVGGGIARDLLVNETPIVLKE
                     DVYAVLAFFSGVLYHLCVVNWKLPQIPTFITIFTISLIIRLLVIKYKINLPNMESKRK
                     V"
     gene            2862..3830
                     /locus_tag="U1099_001298"
     CDS             2862..3830
                     /locus_tag="U1099_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEM4867617.1"
                     /translation="MVSITKEQHLDMFLKMQQIRDVDMKLNKLVRRGFVQGMTHFSVG
                     EEAAAVGPIAGLTDEDIIFSHHRGHGHVIAKGIDINGMMAELAGKATGTSKGRGGSMH
                     LANVEKGNFGSNGIVGGGYALAVGAALTQQYLGTDNIVIAFSGDSATNEGSFHESMNL
                     AAVWNLPVIFFITNNRYGISTDISYSTKIPHLYQRAAAYGIPGHYVEDGNDVIAVYEK
                     MQEVIEYVRAGNGPAMVEVESYRWFGHSTADAGVYRTKEEVNEWKAKDPLKKYRKYLT
                     ENKIATDEELDAIEAQVAEQVEASVKFAQESPDPDISVAYEDVFVD"
     gene            4117..5112
                     /locus_tag="U1099_001299"
     CDS             4117..5112
                     /locus_tag="U1099_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEM4867618.1"
                     /translation="MAETKVMALREAINLAQSEEMRKDEKVFLMGEDVGIYGGDFGTS
                     VGMLDEFGPKRVRDTPISEAAIAGSAVGAAQTGLRPIVDLTFMDFITIALDAIVNQAA
                     KTNYMFGGGLKTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGTANDAKGL
                     LKSSILDNNPVIFLEPKALYGKKEEVNLDPDFYIPLGKGEIKREGTDVTIISYGRMLE
                     RALKAAEEVAAEGISVEVVDPRTLIPLDKELIIESVKKTGKVILVNDAYKTGGFIGEI
                     ASIITESEAFDYLDAPIIRIASDDVPVPYANILENAVLPNVEKIKAAIYKQVNKG"
     gene            5452..6840
                     /locus_tag="U1099_001300"
     CDS             5452..6840
                     /locus_tag="U1099_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEM4867619.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGATVPVTEVIAYIGAEGETVEAGASSAPAVEPAAAIEEV
                     PAGRTPVIVAPATAAKPQGSGKVRATPAARKLARELGIDLGLVPGTGANGRVHKVDVE
                     DFKGAAPKATPLAARIAADQGVDLSTLTGSGVNGKIVKNDVLAVLAPAAVETAAPAPK
                     AEEKPAKELPEGVEIIKMSPMRKAISKGMVNSYLTAPTFTLNYDIDMTNLMALRKQVL
                     EPIMNKTGLKVTFTDLIGLAVVRTLMKEEHRYMNASLINDAQEIELHKFVNLGIAVGL
                     DDGLVVPVVHGADKMSLSDFVVASKDVIKKAQSGKLKGAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVASTVQTPVVIDGEIKIRPIMALCLTIDHRIIDGMNGAKFMVDL
                     KNLLENPLELLI"
     gene            7114..8874
                     /gene="lpdA"
                     /locus_tag="U1099_001301"
     CDS             7114..8874
                     /gene="lpdA"
                     /locus_tag="U1099_001301"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEM4867620.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGTTVPVTEVIAYLGAEGESVEVGSAPAPAEVAQATADLK
                     AAGLEVPAAPAAAPQAPKAELAADEYDMVVVGGGPAGYYAAIRGAQLGGKIAIVEKSE
                     FGGTCLNKGCIPTKTYLKNAEILDGLKIAAERGINLASTNYTVDMDKTVDFKNKVVKT
                     LTGGVQGLLKANKVTIFNGLGQVNPDKTVVIGDKVIKGRSIILATGSKVSRINIPGID
                     SKLVLTSDDILDLREIPKSLTVMGGGVVGVELGLVYASYGTEVTVVEMADRIIPGMDR
                     EVSVELQKVLSKKGMKFLTSVGVSEIVEANNQLTIKLNDGSEIISEKALLSIGRVPQL
                     AGLENLHLELDRGRIKVNAYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHG
                     NHHKAKLDFTPAAVYTHPEIAMVGLTEDQAIEKYGKENILIGRNSFTGNGRAIASNEA
                     HGFVKVIADKKYHEILGVHIIGPVAAEMINEAATIMESELTVDDVAASIHGHPTFSEV
                     MYEAFLDVLGVAIHNPPKRK"
     gene            9110..9955
                     /locus_tag="U1099_001302"
     CDS             9110..9955
                     /locus_tag="U1099_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4867621.1"
                     /translation="MRKIIFLDVDGTLVDYHNRIPESAIRAIRQARENGHLVYVCTGR
                     SRAEMQPELWEIGLDGMIGGNGSYVEHQGQVIMHQLLSEEDSRAIVDWLHERGLEFYL
                     ESNNGLFASENFRERARETLRIYSMNKGKTAEEVATQEVDDVIHGMIFDSQLYRNDLN
                     KVSFVLDSYQDHLDSKEAFPQLVANTWGGRGESALFGDLGVKDIDKAHAISVLLDHLG
                     ASQADTIAFGDAKIDISMLDYCAVGVAMGNGGAEILAMADMITDDVEEDGLYNAFERL
                     GLLDK"
     gene            10122..10955
                     /locus_tag="U1099_001303"
     CDS             10122..10955
                     /locus_tag="U1099_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM4867622.1"
                     /translation="MQIEKVYNNNVIQAIDQQGRELIVMGKGLGFQKKAGEELDTSKI
                     EKTFVLQNDYQQSDLSSLYLQMESGEVEVVNAIINRAEETLEVQFDLSLYLALADHLH
                     FVFQRCREGIFIENPLSWEVRKFYPKEYQIGLEALQLVQERLGLELGKGEASSIALHL
                     VNAQKNGAFGKETQTISKIVTQILDIVRLHFGSVTYEEDTSYHRFVTHIQYFAQRVAN
                     GVVEGVNDAFLYEQVMANYPDSFACTTKIRQHIAEVYHFEMSKDEQVYLTIHIQRLKT
                     S"
BASE COUNT         3320 a         2105 c         2567 g         3012 t
ORIGIN      
        1 ccaaagatag agcacaggag gaagttatga aaacatttat tagtaaactg atgttagtgt
       61 ccacggttgt actattagct gcttgtcagt ctaatcaggt aaaaggtggt gagagttcta
      121 gttctagtgc ttcaagccaa gtttcagatg gttctagttt gcagcaagca tcaacaatag
      181 catctagctc tgagacgcaa gcatcctcag tgaccactcc tgagctaagt gaagtatacc
      241 aatcagttat tgatcgttat caagcgaata tggggcagtc tgctgaggca attaatcagg
      301 atgaggtcag tagctatctg agcttattaa ctagccaggg gcaagaatac agtggaatat
      361 tttatagttt gtatgatgtc aaccatgatg gtacagagga gctgcttcta gctttggaca
      421 agtcgggaga atatgtctta attgatctct atactcagct tgccgatgag agtctacgtt
      481 tggtggataa tttccgcaat cttggatttg aaattggacc gaacgcccta cttcgtcctt
      541 tacaggatgg aacatactta tttgaaggag atggtatttt tcgcatttat caatacaatg
      601 cgatgattcc aggtttaaaa cggatttctg aatcagatac caatcctgaa acttctccct
      661 tgcttgagtt gaaaagcttg cattgggtta agctatagat tttatcaatg attattccta
      721 ggctgggttt cccagccttt tgtagtttct aagaacttta agttttcttt tggtaatctt
      781 taatctttct tttaggaagg aaaggtaaac taggagcata tcaggaaggg atacgacttc
      841 ctaaagaaag aaaaggaatc tacattatga aaacaaatct taaaaaatta ctttactctg
      901 gtgttactct tatgagcctt ggtgtcttag cagcatgttc atctacttca agttcaacga
      961 ctacttcaag ctcagcagca actagtcaga caagtgtagc gacaacttca aataatagca
     1021 cttcatcaga ttcaagctcg tcaactattg actggtcagc ccttccaacg acagaagtga
     1081 cgctttcaaa tgatggcttg aaaattactg agggtggaac ctatattttg actggttcaa
     1141 caacagctgg tgtaacggtt gaaacagatg ccaatgttcg catcattcta gcaggtgctg
     1201 agatttcaag ttcagataca gcagcaatca atgttattaa tgctgacaat gttgaattgg
     1261 aaatcaaaga tggtacgacg aatacagtga aagataccag caatcataca gacaccaata
     1321 ttgaaggggc tatccatgtt gaagctgatt tgaccattac aggaaatggt agcctgaccg
     1381 ttgaaggtaa cttccaagac ggcatcgtat cgacggacga ccttgtcgtg aattcaggaa
     1441 acattaccgt cactgcagtg gatgatggta tccgtggtaa ggattcgttg acaatcaatg
     1501 gtggtgaaat tactgtaaca gcaggtgatg atggtattaa agcggagacg gctttgacaa
     1561 ttgacggagg caccattaca gtatcagaat cagcagaagc tcttgaagga acgaatataa
     1621 ccatcaatgg tggtacatta gatgtttacg gttcagatga tgctattaat gcggcaagta
     1681 caacgtcatc agaaatcttc atcaaggtaa ctggcggtga cttgaaagta gctgtaggta
     1741 gtggagatac ggatgccttt gatgctaatg gagacattta tatatctggt ggtacaatcg
     1801 atgtaacagc tcaatctgct tttgacttcg acggaactgc tgaattgaca ggaggtactg
     1861 tgacagttaa tggtgagcag attactcaaa tcacagcaac aggaccaggt gctggtggcc
     1921 acggtggctg gtaatcaata ataatacaaa aaggtctgag ttgacgaact cagacctttc
     1981 gtttgctttc catattggga agattgattt tgtatttaat taccaataat cgaatgatta
     2041 aagaaatggt aaaaatagtg atgaaggttg gaatttgtgg taatttccaa tttacaacgc
     2101 aaagatgata gaggactcct gagaaaaatg caaggacagc atagacatct tctttaagaa
     2161 caatgggtgt ttcattgacg agtaaatcac gggcgattcc gcctccgaca cctgtcaaag
     2221 ctgccagaat accactggtt agagcgttta gctggagaga gatgccagta ctggctccaa
     2281 tgagggcgaa gatggctaga ccgattgcat caaagataag gtttgtctgg gaaaggaaat
     2341 gaatcatctt cttatcaagt ggtttgtcct gctttagatt gataacaatc aagtacatgc
     2401 taatcgatac tccgagagat aaatagatgg ctgtaggatc agttaaggcg gcaggaatgc
     2461 gattggccat cgtatctcga ataattcccc caccaacggc ggtaatgatg ccgagcaagc
     2521 taattccaaa aatatctaac tttttcttaa atccttttac ggcccccgaa acagcaaatg
     2581 cgatagtgcc tatgtagtta caaatcataa gaaataaatc gaattccata agaacctccg
     2641 tacctatatt aacagaaaga aagatggcgt gcaagcttat tttaaagttt gtgaaaaaag
     2701 tttcaaacaa gtaaagtgaa tcttttaatg atttgtgaaa aaacgcacta aatcaaggtt
     2761 ttcacaaaaa tttctgtaaa aagaaaacgg ttcaattact tgctatttgt gaaatattgg
     2821 tctatcatag tacagaaaga gaaaaacctt tggaggaaat tatggtatct atcacaaaag
     2881 aacaacattt ggacatgttc ttgaaaatgc aacaaatccg tgatgttgat atgaaattaa
     2941 acaaattagt gcgtcgcggt ttcgtacaag gtatgactca cttctcagtc ggtgaagaag
     3001 ctgcggctgt tggacctatt gctggattga cagatgagga catcatcttc tcacaccacc
     3061 gtggtcacgg tcacgttatt gcaaaaggta ttgacatcaa cggtatgatg gctgagcttg
     3121 ctggtaaggc aactggtaca tcaaaaggcc gtggtggttc tatgcacttg gccaatgttg
     3181 aaaaaggaaa ctttggttca aacggtatcg taggtggtgg ttatgccctt gcggtgggtg
     3241 cggcccttac gcaacaatac ctcggtacag ataacattgt tattgccttc tcaggtgatt
     3301 cggctactaa cgaaggttca ttccacgaat caatgaactt ggcagctgtt tggaacttgc
     3361 cagttatctt cttcattaca aacaaccgtt acggtatttc aactgacatt tcttattcta
     3421 ctaagattcc tcacctctac caacgtgcag cagcatacgg tattccaggt cattatgttg
     3481 aggatggtaa cgacgttatt gctgtttatg aaaaaatgca agaagtcatc gagtacgttc
     3541 gtgcaggtaa cggtccagcc atggtcgaag ttgaatcgta ccgctggttt ggtcactcaa
     3601 ctgccgatgc aggtgtttac cgtactaaag aagaagtcaa tgagtggaaa gcaaaagacc
     3661 cacttaagaa ataccgcaaa tacttgacag agaacaagat tgcgacagac gaagaattgg
     3721 atgccatcga agcacaagtt gcagaacaag tagaagcatc tgtgaaattc gcacaagaaa
     3781 gcccagatcc agacatctca gtagcttatg aggatgtgtt tgtagactag ggtgtgagga
     3841 cgttaaagag gcacagaaaa attagggagt cagctagtga tgtggacatc actaatggct
     3901 catctatttt tccaagcctc tagtcagaac ctaactacaa acgtaagaag tttgactaag
     3961 ctctaaatag caagtcaaac tagggtggac tacctactca agttcgagta caattctaag
     4021 gtgtcttaag ctgggacaat aaacaattac atcgtagtaa ttgtttggtt ttccttagtt
     4081 cagtatagat aaattgatat aaattaggag taaaaaatgg ctgaaacaaa agtaatggcc
     4141 ttgcgtgaag cgattaactt ggctcaaagc gaagaaatgc gtaaggatga aaaagtattc
     4201 ttgatgggtg aagacgtcgg tatctacggc ggtgactttg gtacatctgt tggtatgttg
     4261 gacgagtttg gtccaaaacg cgttcgtgac acgcctatct ctgaggctgc aatcgctggt
     4321 tctgcggttg gtgcggctca aacaggtctt cgtccaatcg ttgacttgac tttcatggac
     4381 ttcatcacaa ttgcccttga tgcaattgtt aaccaggctg ccaaaaccaa ctacatgttt
     4441 ggtggtggct tgaagacacc tgtaaccttc cgtgtggcat ctggttcagg tatcggttca
     4501 gcggcacagc actcacaatc tcttgaagct tggttgactc acattccagg tatcaaggtg
     4561 gttgcaccag gtacagctaa cgatgcaaag ggtcttttga aatcatctat ccttgataac
     4621 aaccctgtta tcttcttgga gccaaaagct ctttatggta aaaaagaaga agttaacttg
     4681 gatccagatt tctacattcc acttggtaag ggtgaaatca aacgtgaagg tacagatgta
     4741 accatcattt catacggtcg tatgttggaa cgtgccttga aagctgctga agaagtggct
     4801 gcagaaggaa tcagcgtaga agtggttgac ccacgtaccc ttatcccatt ggataaagaa
     4861 ttgatcatcg aatctgtgaa gaaaactggt aaggttatct tggttaacga tgcttacaaa
     4921 acaggtggtt tcatcggtga aatcgcatca atcatcacag aaagcgaagc ctttgactac
     4981 ttggatgcac caatcatccg tatcgcttca gacgatgtac cagttcctta cgcaaacatt
     5041 cttgaaaacg cagtattgcc aaacgtagag aaaatcaaag cggcaatcta taagcaagta
     5101 aacaagggtt aatcttatcg tcatccgctt atcttttctg gctgtgttga tttggattgc
     5161 ctaacgtcag ttatgcctgc actaaatcgc tttatcagaa aagcaatcgg aaagacgata
     5221 accctaatat gctagaaata aaatgttcta gcatgactaa gaatgcttgg aaaagaattg
     5281 agccgcttcg attatcgaag tctggtttgg ttcttgacaa ggtaagaaac tgaaagaaag
     5341 gaattgaacc cgtccggaaa actcggaaaa aagataaatc gttacgaaaa tcaggatttt
     5401 cgacgatttc ctagattccc tcgttttctg ttcggacttg gtatcttaat aatggccatt
     5461 gaaatcatta tgcctaagct cggtgtggac atgcaagaag gtgaaatcat cgagtggaaa
     5521 aaacaagagg gcgatttcgt caacgaaggc gacgtcatct tggaaatgat gtctgacaag
     5581 accagcatgg agctggaagc agaagagtca ggtgtcctat tgaaaattgt tcacggaaac
     5641 ggtgctaccg ttcctgtaac agaagttatt gcctacatcg gtgcagaagg tgaaaccgtc
     5701 gaagcaggcg cttcatctgc tcctgctgtt gaaccagcag cggctattga agaagtacct
     5761 gcgggtcgta caccagtaat cgttgctcct gcaactgctg ccaaaccaca aggtagtggc
     5821 aaggtgcgtg caacgccagc tgcgcgtaaa ttggcgcgtg agcttggaat cgacttgggt
     5881 cttgttccag gaacaggtgc taacggtcgt gtccacaagg ttgacgtcga agacttcaag
     5941 ggagcagctc caaaagcaac tccgcttgca gcccgtattg cagcagacca aggtgttgac
     6001 ttgtcaaccc ttacaggttc aggtgtcaac ggtaagattg tcaagaacga cgttcttgca
     6061 gtacttgctc ctgcagctgt agagactgct gctccagcac cgaaagcaga agaaaaacca
     6121 gctaaagaat tgccagaagg cgttgaaatc atcaagatga gcccaatgcg taaggcgatt
     6181 tcaaaaggta tggtcaactc ttacttgact gctccgacat ttacacttaa ctacgatatt
     6241 gacatgacca acctcatggc acttcgtaag caagtccttg agccaatcat gaacaagact
     6301 ggtttgaaag taacctttac agacttgatc ggtcttgcgg ttgttcgtac tttgatgaaa
     6361 gaagaacacc gctacatgaa cgcatctttg attaacgatg ctcaagaaat cgaattacac
     6421 aagttcgtca accttggtat cgcagtgggt ctagatgatg gcttggtggt tccagttgtt
     6481 cacggcgcag ataagatgag cttgtctgac tttgtggtgg cttcgaaaga tgtcatcaag
     6541 aaggctcaat ctggtaagtt gaagggggct gaaatgtcag gttcgacctt ctctatcacc
     6601 aacttgggta tgtttggtac taaaaccttt aacccaatca tcaaccaacc aaactcagca
     6661 atccttggtg tagcatcaac agttcaaaca ccagttgtta ttgacggcga aatcaaaatc
     6721 cgtccaatca tggcactctg cttgaccatc gaccaccgta tcattgatgg tatgaatggt
     6781 gctaagttca tggttgactt gaagaacttg ctggaaaacc cattggaatt gttgatctga
     6841 gataagatgc aaaaggcgtt aagaaaaccg tatgaaaata ggaaatcaac gatgtgcctt
     6901 gaggcacgag gcgatttatc tttttcaata ggtttttagc ctgtgctcaa tttggtaaag
     6961 agaaaaaata aagaaaggga ataagagcga gagagtttta gcaattgaac ccgagcggaa
     7021 agctcggaaa aaagataaat cgtttagaga atcaggattt tctacgattt cctatttttc
     7081 agtcgctttc tcttcgctct tggtatctta actatggcaa ttgaaattat tatgcctaaa
     7141 cttggtgtag atatgcaaga aggcgaaatc atcgagtgga aaaaacaaga gggtgatttt
     7201 gtcaacgaag gtgatgttat cttggagatg atgtcagaca agacaagtat ggaattggaa
     7261 gcagaagaat caggtgtact cttgaaaatc gttcatggta acggtacaac tgttcctgta
     7321 acagaagtca ttgcttacct tggtgcagaa ggcgaatcag ttgaagttgg tagcgcacct
     7381 gctccagctg aggttgcaca agcaactgcc gacttgaaag cagctggttt ggaagtgcct
     7441 gcagctcctg cagcagctcc acaagctcct aaggctgaat tggcagctga cgagtacgac
     7501 atggttgttg tcggtggtgg tcctgctggt tactacgcag ctattcgtgg tgctcaattg
     7561 ggcggtaaaa tcgctatcgt tgagaaatca gaatttggtg gtacctgctt gaacaagggt
     7621 tgtatcccaa ctaagaccta ccttaaaaac gctgaaattc ttgacggctt gaagattgct
     7681 gctgagcgtg gtatcaacct tgcttcaaca aactacaccg ttgatatgga caagacagtt
     7741 gacttcaaga acaaggttgt gaagacattg actggtggcg ttcaaggtct cttgaaagct
     7801 aacaaggtaa cgatcttcaa cggtcttggt caagtaaacc ctgacaagac tgttgtcatc
     7861 ggcgacaagg tcatcaaagg tcgcagcatc attcttgcaa ctggctctaa ggtttctcgt
     7921 atcaacatcc caggtattga ttctaaattg gtgctaactt ctgatgatat ccttgacttg
     7981 cgtgaaattc ctaagtcact cactgttatg ggtggtggcg tagtcggtgt ggaacttggt
     8041 ttggtttacg cgtcttacgg tactgaagta acagttgttg aaatggctga ccgtatcatt
     8101 ccaggtatgg accgagaagt gtctgttgaa ttgcaaaaag tcctttctaa gaaaggtatg
     8161 aaattcttga catcagttgg agtatctgaa attgttgaag ccaacaatca attgacaatt
     8221 aaattgaacg atggttcaga aattatctct gaaaaagctc ttctttcaat tggacgtgta
     8281 cctcaattag ctggtcttga aaatcttcac cttgagttgg atcgcggtcg tatcaaggtt
     8341 aatgcttacc aagaaacttc tatcccagga atttatgcac ctggtgatgt caacggtact
     8401 aagatgttgg cacacgctgc ttaccgtatg ggtgaagtgg cagctgaaaa tgctattcac
     8461 ggtaaccacc acaaggctaa attggacttc acaccagcag cggtttacac acacccagaa
     8521 atcgctatgg ttggtttgac tgaagatcag gctatcgaga aatatggtaa agaaaacatc
     8581 cttatcggtc gcaacagctt tactggtaac ggtcgtgcta ttgcttctaa cgaagcacat
     8641 ggtttcgtaa aagtaattgc ggacaagaaa taccatgaaa tccttggtgt tcatatcatc
     8701 ggtccagttg cagctgaaat gatcaatgaa gcagcaacta tcatggaatc tgagttgaca
     8761 gttgacgacg tggcagcatc catccacggt cacccaacct tctcagaggt tatgtatgaa
     8821 gccttcctgg atgtgcttgg tgttgcaatc cacaacccac caaaacgtaa ataatagcat
     8881 tcagtttatc ttttattact tcgttaactc gatttgccgt actctagtac agcctgcatc
     8941 tcgttgccta gtactaaaag caaactgaaa tgctaacatc taaaagaaag tccatatggc
     9001 tttctttttt gtatgttcat agtatagtat tagtctatcg cagtttagga ctgttatttg
     9061 atagaaaaac agactatact tgtagtagaa aaaggcaagg agaaaacaga tgcgcaagat
     9121 tattttttta gatgtggacg ggaccttggt tgactatcat aatcggattc cagaatctgc
     9181 cattcgtgct attcgacagg ccagagaaaa tggacacctg gtctatgtct gtacaggtcg
     9241 aagccgagcg gaaatgcaac cagaactatg ggagattggc ctggacggaa tgattggtgg
     9301 caacggctct tatgtcgagc atcaaggtca ggtcatcatg caccagctcc tatcagaaga
     9361 ggatagcaga gcgattgtgg actggctcca tgaacgcggc ttggaatttt atctggaaag
     9421 caacaacggg ctctttgcca gtgaaaattt ccgtgaacgt gcccgtgaaa ccctgcgaat
     9481 ttattccatg aacaagggga aaactgcaga agaggtggcc actcaggaag tagatgacgt
     9541 tatacacggc atgatttttg atagtcaact ctaccgcaat gatctcaaca aagtcagttt
     9601 tgtactagac tcctatcagg atcatttgga ctctaaagag gcctttccgc aactggtcgc
     9661 aaacacctgg ggaggtcgcg gagagtctgc cctctttggc gatttgggtg taaaagatat
     9721 tgacaaggcc catgctattt ctgttttgct ggatcactta ggagctagtc aggcagacac
     9781 tatcgccttt ggtgacgcta aaattgatat ttccatgctg gactattgtg cggttggggt
     9841 cgctatgggc aatggcggag cggaaatctt ggctatggca gatatgataa cggacgatgt
     9901 agaagaagac ggcctctaca atgcctttga aagattggga ctacttgaca agtagctctc
     9961 agaatccctt gcaaaaccgc ttacaatctg ataagataag tgtaggattg ttactgataa
    10021 gcaggcaaaa cctaagtaga tagtgaaggg tctggcaaca gtcgtatttg ctatgtgctt
    10081 gggttctttt ttatcagtcg aaaaaagagg cggtaaagta gatgcagatt gaaaaagttt
    10141 ataacaacaa tgtcattcaa gcaatagatc agcagggccg tgagttgatt gtcatgggga
    10201 aaggattagg ttttcagaaa aaagcagggg aagaattaga caccagcaaa atcgagaaaa
    10261 cctttgtgct acaaaatgac taccaacaat cagatttatc gagtctgtac ctgcaaatgg
    10321 agagcggtga agtcgaagta gtcaatgcca ttatcaatag ggcagaagaa actttagaag
    10381 ttcagtttga tttgtctttg tatctagcct tagcagacca tttacatttt gtatttcagc
    10441 gatgccgtga aggtatcttt attgagaacc cgctgtcttg ggaagtacgt aagttttatc
    10501 cgaaggaata ccaaattggt ttagaagcct tgcagttagt gcaggagcgt ttaggcttgg
    10561 aactaggaaa gggagaggca tcgtctatcg ctctccatct ggtaaatgct cagaagaatg
    10621 gtgcttttgg caaggaaacc caaaccatta gtaaaatcgt gactcagatt ttagacattg
    10681 tccgccttca ttttggtagt gtgacctacg aagaagatac cagctatcat cgatttgtaa
    10741 cacatattca gtattttgct caacgtgtgg ccaatggtgt ggtcgaaggg gtcaatgatg
    10801 catttttgta tgaacaggtg atggctaact atcctgattc atttgcttgt acgactaaga
    10861 ttcgtcagca tattgcggaa gtttaccatt ttgaaatgtc caaagatgaa caagtctatt
    10921 tgaccatcca tatccagcgc ttaaaaacga gttagaaaaa aatcaaaaaa actttagaaa
    10981 acgcttgcaa aaaaatttgt aatg
//
LOCUS       DASFSW010000070        10962 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.070, whole genome shotgun sequence.
ACCESSION   DASFSW010000070 DASFSW010000000
VERSION     DASFSW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.070"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(110..658)
                     /locus_tag="U1099_001304"
     CDS             complement(110..658)
                     /locus_tag="U1099_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEM4867623.1"
                     /translation="MISLAIILILAWSFYIGYSRGLVLQAFYSMGSIIALVVATATYK
                     KLASFLYLWVPFANATQGSSNYYFDEKYLFDLDMVFYAGLAFLLIYVLVYAIVRFIGI
                     FVHLLEAFNPDTKTTNLISGALAVLVTFISLQIVMVLLSTIPLAMIQDKLHSSFLANI
                     MIQYTPFTSSFLKSLWLSNIAG"
     gene            complement(655..966)
                     /locus_tag="U1099_001305"
     CDS             complement(655..966)
                     /locus_tag="U1099_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867624.1"
                     /translation="MANLNRYKFTFGEKQLTLTTEHDNLFMEEIERIAQEKYQAIKEK
                     MPTADPETLALLLAINALSVQLTREMAFEQKEQELASVKEKVLKKNVTLIDLDELEDK
                     V"
     gene            complement(1024..1737)
                     /gene="treR"
                     /locus_tag="U1099_001306"
     CDS             complement(1024..1737)
                     /gene="treR"
                     /locus_tag="U1099_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEM4867625.1"
                     /translation="MKKYQEIYNDLKEKIRTNVYPAESSLPTEQQLQEIYGVSRDTVR
                     KALAILTEGGLIQKVQGRGSMVLKQEILNFPVSGLTSYQELTNVLQLSTKTDVVSLDM
                     ITVNSSLSHLTGFEPYSKVWKVVRTRSIDGKVSVVDTDYLAVDVVPELTTAIAEKSIY
                     EYLENKLGLDIAYAQKEITVEPTNREERELMQSKDDYLVLIKSRVYLGDTQQFQYTES
                     KHKIDKFRFVDFARRKHSL"
     gene            1970..3964
                     /gene="treP"
                     /locus_tag="U1099_001307"
     CDS             1970..3964
                     /gene="treP"
                     /locus_tag="U1099_001307"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEM4867626.1"
                     /translation="MGKFEKEAKQLLEAIGGKENVNAVTHCATRMRFVLADEKKANVK
                     VIEDIPAVKGTFTNAGQFQVIIGNDIPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNAI
                     QRVMTMLAEIFTPIIPALIVGGLILGFRNVLEGVQMEWLGQAMKDGQLVFDADGNPVW
                     NTIVQVSPFWNGVNHFLWLPGEAIFHFLPVGITWSVSRKMGTSQILGIVLGICLVSPQ
                     LLNAYAVPGTDAATIASDWSWNFGAFSIARIGYQAQVIPALLAGLALSYLEIFWRKVV
                     PEVVSMIFVPFLSLLPAIILAHTVLGPIGWTIGQWLSTVVLAGLTGPLKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADAGGTGLWPMIALSNIAQGSAVFAYFLMNRHNEKE
                     AQVSLPATISAYLGVTEPALFGVNVKYVYPFVAAMIGSSIAGLLSVTFNIQAASIGIG
                     GLPGILSIKAEYWGAFFMTMIVAIVVPMILTAVFKRTGAFSKKEETVEEVVAPTEAVV
                     ETGAAIELISPLTGQTKELSQATDPVFASGVMGQGVLIDPTEGLLVAPVDGEISVLFP
                     TNHAVGITATNGVEFLMHIGMDTVGLEGKGFTAHVKQGDKVKAGDKLISFDIDVIKAA
                     GLVAETPVIVTNQTDFDTQVIGNLPRAISQGEAILTVTKN"
     gene            4045..5670
                     /gene="treC"
                     /locus_tag="U1099_001308"
     CDS             4045..5670
                     /gene="treC"
                     /locus_tag="U1099_001308"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEM4867627.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLKELGID
                     MIWLNPFYPSPQRDNGYDISDYTAVNPDFGTMADFEEMVTVGKELGIEFMLDMVLNHC
                     STDHEWFQKALAGDKYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELFKVVNFWKDKGVKGFRFDVINLIGKDEVLEDCPINDGKPAYTDRP
                     ITHDYLKMMNNATFGAEKGFMTVGEMSATTIENCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMDFDFEELKQLFHTWGEEMSAGNGWNALFYNNHDQPRALNRFVDVENFRK
                     EGATMLAASIHLSRGTPYVYMGEEIGMIDPDYDSMDDYVDVESINAYQMLLDQGHAPE
                     QAFKIIQAKSRDNSRTPMQWDASDNAGFSTGTPWLKAGKSYPTINVEAEKTGPIFTFY
                     QELIRLRKELPLISEGDYKAAYKDSQKVYAFERLLNGEKLLVLNNFFAEEVELDLADD
                     YAHGQVLISNYPDNKLGKKITLKPYQALAIQVK"
     gene            complement(6083..7258)
                     /locus_tag="U1099_001309"
     CDS             complement(6083..7258)
                     /locus_tag="U1099_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM4867628.1"
                     /translation="MKRKRTNKPQHMRRKRKTPIMKNNKKMLYTSSLALSLFSTGMIS
                     TNVLAIEWAPRTVSEISPEIVQEEGKMTYTVQYGDTLSAIALAMNIDMDLLAKINQIA
                     DVNLIFPDTVLTTTVDQNNQVTQVEIEAPVQGNTNETVQAIVDLTTNQVTVEDTVVPL
                     DQISSVTDSAPVEEVVEQPVAEASVEEVVEQPVVEAPVEEVVEQPVAEASVAEASVEE
                     VVEQPVVEAPVEEVVEQPVVEAPVEEVVEQPVVEAPVEQPVVETPQVTALSTTTTSTS
                     AYDVGLQPQVAAFRAEVANAFGITSFSGYRPGDSGDHGKGLAIDFMVPESSALGDQVA
                     AYAVANLASKNINYIIWKQRFYAPYDSIYGPAYTWNLMPDRGSVTENHYDHVHVSFN"
     gene            complement(7607..8590)
                     /locus_tag="U1099_001310"
     CDS             complement(7607..8590)
                     /locus_tag="U1099_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM4867629.1"
                     /translation="MEVQMKYKWATLGTGVIANELVQALQAMVGNLYSVANRTYDKGV
                     EFAQKYGIEKVYREIDEVFEDPEVDIIYISTPHNTHINYLRKALKAGKHVLCEKSITL
                     NSEELAEAIQLAEENQVILVEAMTIFHMPIYRQLSEVVASGKLGDLKMIQMNFGSYKE
                     YDMTNRFFNKNLAGGALLDIGVYALSFVRWFMTEKPNQVLSQVKLAPTGVDEQVGILL
                     SNDAGEMATIALTLHAKQPKRGTIAYDKGYIELYEYPRGQKAVITYTEDGSQEVIEAG
                     DTAKALSYEVADMEKAVAGIENTMHLAYTQDVMEIMTQLRKEWGLVYPEEM"
     gene            8675..9565
                     /locus_tag="U1099_001311"
     CDS             8675..9565
                     /locus_tag="U1099_001311"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEM4867630.1"
                     /translation="MNTVVLKASAQQKQAMMAHYDRYRQTSKNPYIEAFFKLTGASLS
                     IYTSGKVVFQGEMAEQEAGLWGYEPESSGQTTVPGQNLPMIGTDEVGNGSYFGGLAVV
                     ASFVRPEDHAFLKSLGVDDSKKMTDQKICQVAPLLKEKIPHQALLLSPKKYNEVIEQG
                     YNAVSVKVALHNQAIFLLLQKGVQPEKIVIDAFTSSQNYQKYLKKEANQFANPVTLEE
                     KAEGKYLAVAVSSIIARAMFLENLVQLGQLVGMNLPSGAGSKSDQVAASILKQYGMAG
                     LNQTAKLHFANTQKAQKLLK"
     gene            9575..10204
                     /gene="lepB"
                     /locus_tag="U1099_001312"
     CDS             9575..10204
                     /gene="lepB"
                     /locus_tag="U1099_001312"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM4867631.1"
                     /translation="MGAIQKTKRSPLVAFLAEWGIFLLFMAAFFASRYFIWNPVSVDG
                     HSMDPTLQHQEKLIMLKTTSIDRFDIVVASETDSDGKEKLIVKRVIGMPGDTIRYEND
                     VLYVNDQKVDEPYLDEYLAAFQKDKLQEVYSYNKQFQAVAQSAEAFTQDANGYVDFTV
                     TVPEGQYYLMGDDRLVSLDSRSVGTFSRENIKGEVVFRMWPLNRIGTVD"
     gene            10268..>10962
                     /locus_tag="U1099_001313"
     CDS             10268..>10962
                     /locus_tag="U1099_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEM4867632.1"
                     /translation="MNEVYFTGTIDRIIFENPSNFYKILLLEIEETDADYDDYEIIVT
                     GTIADVIEGEDYRFYGNLVTHPKYGQQLQISRYERSKPTSAGLVKYFSSEHFKGIGRK
                     TAEKIVELYGEDTIDKILAEPEKLTQITGLSSKNMQAFVEKLRLNYGTELILAKLAEY
                     GIPNKLAFQIQDQYKEKTLQIIEENPYQLVEDVQGLGFTIADRIAENLGIASDSPQRF
                     RAGMLFSLIHRSM"
BASE COUNT         3170 a         2511 c         2183 g         3098 t
ORIGIN      
        1 tagactgctc gagttctttc ccactcccac tcatcaaatc aagtcaacaa tgtctgattt
       61 tgattttcga agagtatcat aaatgacgat aaaaaggata aggtctccct taccctgcta
      121 tattactcaa ccagagagat tttaaaaagc tactagtgaa tggtgtgtac tgaatcatga
      181 tgttggcaag aaaactactg tgcaatttat cttgaatcat cgctaacgga atagttgata
      241 gcaagaccat gacaatctga agagaaataa aagtcaccaa gacagccaag gcgccgctta
      301 tgagattggt cgtttttgta tcaggattaa aagcctctaa tagatggaca aatattccta
      361 tgaaacgtac aatagcataa accaagacgt atatcaaaag aaaggccaat cccgcataaa
      421 ataccatatc taggtcaaag agatatttct catcaaaata atagttggag ctcccctgag
      481 tagcattggc aaatggcacc cagagataaa ggaaggatgc caatttttta taagtagccg
      541 ttgcaaccac cagagcaata atacttccca tgctataaaa cgcctgtaga actagaccac
      601 ggctgtagcc aatgtagaaa ctccatgcca aaatgagaat aattgctaat gaaatcatac
      661 cttgtcctca agctcatcta agtcaatcaa ggtcacattc tttttcaaga ccttttcttt
      721 gacgcttgca agttcttgtt ccttttgttc aaatgccatc tcgcgcgtta gttgaacgga
      781 tagagcattg attgccaaca aaagcgcgag ggtttcaggg tccgccgtcg gcatcttttc
      841 tttgatggct tggtattttt cctgtgcaat acgctcaatc tcttccatga ataaattatc
      901 atgctcagtt gtcaaagtta attgcttctc gccaaatgta aatttatatc gatttaagtt
      961 tgccatatct tacctcttct tctatctatt atagcgtaaa aaacttgatt tggctaatct
     1021 ctcctataaa gaatgctttc tacgagcaaa atctacaaag cggaatttat caattttatg
     1081 cttgctttct gtatattgga attgttgggt atcaccgaga tagacacgag atttaatcaa
     1141 gaccaaataa tcatccttcg attgcattag ctcacgctct tctcgattgg tcggctctac
     1201 cgtaatctct ttttgtgcat aagcaatatc aaggcctaac ttattttcta ggtattcgta
     1261 gatggatttt tcagcaatag cagttgtcaa ctctggcacg acatcgacag caagataatc
     1321 tgtatccaca acggagacct taccgtcaat tgaacgtgta cggacaactt tccacacctt
     1381 gctatacggc tcaaagcctg tcaagtgcga aaggctacta ttaacggtaa tcatatctaa
     1441 gctgacaaca tctgtcttgg tagaaagctg gagaacattt gttaattcct gataggaagt
     1501 taaacctgaa actgggaaat tgagaatttc ttgcttaagg accattgaac cacgcccttg
     1561 cactttttga atcaaacctc cctcagtcaa aatcgccaac gccttacgaa ccgtatcacg
     1621 actaacacca tagatttcct gaagctgctg ttctgtcggt agggagcttt ctgccggata
     1681 aacatttgtc cgtatttttt cttttaagtc attataaatt tcttggtatt ttttcatcat
     1741 ttcctcgcaa attcatctgt caaataacat acaactatca acattataac aaaattcatt
     1801 tcaaaattaa aaaaactaag caaaaacgct tgcattattt tttgaaatcg gatacaatga
     1861 taacacaaaa gaaatttgtc cgtacaaatt tcaaaaaaga catacttttg ggagatgtct
     1921 aaaaaatata gggtgacgga ttccataagc cgtctagaag gaaaaatata tgggaaaatt
     1981 tgaaaaagaa gccaaacagc tcctagaagc tattggcgga aaagagaatg tcaatgcagt
     2041 aacacactgt gcaacacgca tgcgctttgt tcttgcagat gagaaaaaag caaacgtaaa
     2101 agtcattgaa gacattccag cagtaaaagg aacatttaca aacgcaggtc aattccaagt
     2161 catcatcggt aacgatatac caatcttcta caatgacttt acagctgtgt ctggtatcga
     2221 aggcgtatca aaagaagctg caaagtcagc cgctaaaagt aaccaaaatg ccatccaacg
     2281 tgtcatgacc atgctggcgg aaatcttcac accgatcatt cctgccctta tcgtcggcgg
     2341 tttgattctc ggtttccgta acgtcttgga aggtgttcag atggaatggc ttggacaagc
     2401 tatgaaagat gggcagttgg tcttcgatgc tgatggtaac cctgtatgga acaccatcgt
     2461 tcaggtttct cctttctgga atggtgtcaa tcacttcctc tggttgccag gtgaagccat
     2521 cttccacttc ttgccagtag gtatcacttg gtccgtatct cgtaaaatgg gaacttcaca
     2581 aatccttggt atcgtccttg gtatctgttt ggtttctcca cagttgctca acgcttatgc
     2641 tgtacctggt acagatgcag caaccattgc cagcgactgg tcttggaact tcggtgcatt
     2701 tagtattgcc cgtatcggtt atcaagcaca agttatccct gccctccttg ctggtttggc
     2761 actgtcttac ctagaaatct tctggcgtaa ggttgttcca gaagtggtct caatgatttt
     2821 cgtaccattc ctatcacttc ttccagcaat tatcttggct cacactgtcc ttggtcctat
     2881 cggttggaca atcgggcaat ggttgtcaac agttgtatta gctggtttga ctggtccatt
     2941 gaaatggctc ttcggtgcga tattcggtgc cctctatgca ccattcgtta tcactggtct
     3001 tcaccacatg acaaatgcca tcgatacaca attgattgcg gatgctggtg gaactggtct
     3061 ctggccaatg attgcccttt ctaacattgc tcaaggttca gcagtatttg cctacttcct
     3121 catgaaccgt cacaatgaaa aagaagcgca agtttctctt cctgcaacta tttctgctta
     3181 tcttggtgtt actgaaccag ccctctttgg tgtcaacgtg aaatacgttt acccattcgt
     3241 agctgctatg attggttcaa gtatcgcagg cttactctct gtaaccttca acatccaagc
     3301 agcttctatc ggtatcggtg gtcttccagg tatcttgtct atcaaagctg aatactgggg
     3361 tgcattcttt atgacaatga ttgttgcgat tgttgtccca atgattttga cagctgtttt
     3421 caaacgtaca ggtgcatttt caaaaaaaga agaaactgtt gaagaagttg tagctccaac
     3481 tgaagcagtt gttgaaactg gcgctgctat cgaattgatt agcccactta ctggtcaaac
     3541 caaagaattg tcacaagcta ctgaccctgt atttgcatca ggtgtcatgg gccaaggtgt
     3601 ccttattgac ccaacggaag gtcttctggt agcacctgtt gacggagaaa tttctgtcct
     3661 ctttccaact aaccatgctg ttggtatcac tgctacaaac ggagttgagt tccttatgca
     3721 cattggtatg gatactgttg gattggaagg taaaggcttt acagcccatg tcaaacaagg
     3781 tgacaaggtt aaagcaggtg acaaattgat tagctttgac atcgatgtca tcaaggctgc
     3841 tggtctcgtc gcggaaacac cagttatcgt gaccaaccaa actgactttg acactcaagt
     3901 tatcggaaat cttcctcgtg ccatctcgca aggcgaagct attctgacag ttacaaagaa
     3961 ttaatgttct aggagggcgc aaggtaactt gcacctccta tcattgctta tgcaagaagg
     4021 aaaaatcaag aaagagagag caaaatgaca atagacaaac gaaaggttgt ttatcaaatc
     4081 tacccaaaat cttacaagga tacaactgga aatggtgtag gggatttacg aggaattatt
     4141 gaaaaactcc catatttaaa agagctaggt attgatatga tttggctcaa ccctttctac
     4201 ccaagtcctc aacgagacaa cggttatgac atttcagatt atacagccgt caatcctgat
     4261 tttggcacca tggctgactt tgaagaaatg gtgactgtcg gcaaagaact aggcatcgaa
     4321 ttcatgctag acatggtcct caatcactgc tcaaccgacc acgaatggtt ccaaaaagcc
     4381 cttgccggcg acaaatatta ccaagatttc tttatcctgc gtgaccagcc aactgactgg
     4441 gtgtccaagt ttggtggcaa tgcctgggct ccttttggag atactgggaa atactacctc
     4501 cacctatttg atgtgaccca agctgacctc aactggcgaa accctcatgt ccgtgaagaa
     4561 ctcttcaagg tggtcaactt ctggaaagac aagggtgtca aaggcttccg cttcgatgtc
     4621 attaacctga ttggtaagga cgaagtcctc gaagactgcc caatcaatga tggcaaacca
     4681 gcttataccg accgcccaat tacccacgac tacctcaaga tgatgaacaa tgccactttt
     4741 ggggcagaaa aaggcttcat gaccgtcggg gaaatgtctg ccaccaccat tgaaaactgc
     4801 attctctaca cagctcctga gcgggaagaa ttgtccatgg ccttcaactt ccaccacttg
     4861 aaagtagact acaaggacgg tcaaaaatgg accattatgg acttcgactt tgaagaactc
     4921 aaacaactct tccacacttg gggcgaagag atgtccgccg gaaatggctg gaacgccctt
     4981 ttctacaaca accacgacca acctcgtgcc ctcaaccgct ttgttgatgt ggagaatttc
     5041 cgcaaggaag gagcaaccat gctggcagcc tctatccacc tgtcacgcgg tactccttac
     5101 gtctacatgg gcgaagaaat cggcatgatt gaccccgatt acgacagtat ggacgactat
     5161 gtggacgtgg aatctatcaa tgcctatcag atgctcttgg accaaggtca cgcacctgag
     5221 caggccttca agattatcca ggccaagtcc cgcgacaatt cccgcactcc gatgcaatgg
     5281 gacgcttctg acaatgcagg cttctcaaca ggcacccctt ggctaaaggc tggcaaatcc
     5341 tatccaacca ttaacgttga agccgaaaaa acaggcccaa tctttacctt ctatcaagaa
     5401 ctcatccgcc tgcggaaaga actccctctg atttcagaag gggactacaa ggcagcctac
     5461 aaggatagcc agaaagtcta cgcctttgaa cgcttgctaa atggcgaaaa actgttggtc
     5521 ctcaacaatt tctttgcaga agaggttgaa ctagatttgg cagacgacta tgcccacggc
     5581 caagtcctca tcagcaacta tcctgacaac aaactcggta agaaaattac cctcaagcct
     5641 tatcaagcac tggcaattca agttaaataa acgataaaac atccgaaaat aacactctac
     5701 gtgtcaattt taattttcct aaaattagaa cccgtcttca cagctcagta ggttacttgt
     5761 gaaggcaagt tcttggttta aggtttcttt taggaatata ttttatactt agtttgcgca
     5821 cgaaacagct atctgggggg agatagttgt ttcttttttt atagtcattt gaattaactt
     5881 tgatacatcg tcactttcgg cttgccatac tctacgaaag tgacttgttt cgcaagtctt
     5941 attttcaacc tagaacagcc tctaggctgt tctagctacc tgcacctcag ttccttgtct
     6001 gaaagttcat tcattcgact atattactac atataaagca ataaccgttt cagataaggc
     6061 tgaaacggtt atttttgtaa gtctaattaa aagatacatg cacatgatcg tagtggtttt
     6121 ctgtaacgct accacggtct ggcatcagat tccatgtata ggctggacca tagatactgt
     6181 catacggcgc atagaagcgc tgtttccaaa tgatatagtt gatattttta gaagctaagt
     6241 ttgcaactgc ataagccgcc acttgatctc ctagagctga gctctcaggc accataaagt
     6301 caattgccaa tcccttacca tggtcgccag aatcaccagg acggtaacct gagaaagaag
     6361 taataccgaa ggcatttgcc acttctgctc ggaaggctgc tacctgaggt tgcaaaccga
     6421 catcataagc actagtactt gttgtagtgg ttgatagggc tgtcacttgt ggagtttcaa
     6481 ctacaggctg ttccacaggt gcctcaacca caggttgctc tacaacttcc tctacaggcg
     6541 cttctactac aggctgctct acaacttcct ccacaggtgc ttctactaca ggttgctcta
     6601 caacttcctc tacagacgct tctgctacag acgcttctgc tacaggctgt tctacaactt
     6661 cctctacggg tgcttctact acaggctgtt ccacaacttc ctctacagac gcttctgcta
     6721 caggttgctc cacaacttcc tctacgggcg ctgagtcggt aactgatgaa atttgatcca
     6781 agggaacaac cgtatcctca accgttactt gattggttgt taggtcaaca attgcctgaa
     6841 cggtctcatt tgtgtttccc tgaacaggag cttcaatctc aacctgagtc acttgattgt
     6901 tttggtcaac agtcgtcgtc agtaccgtat cagggaaaat caagttgaca tctgcaattt
     6961 gatttatttt cgccagcaag tccatatcaa tattcatagc taaggcgatg gcagataagg
     7021 tatctccata ctgaacagta taggtcatct ttccttcttc ttgtacaatt tctgggctaa
     7081 tttcagaaac agtacgtgga gcccattcga tggctaaaac atttgtcgaa atcatccctg
     7141 tactaaagag ggaaagagcc aatgaagatg tgtataacat ctttttattg tttttcatga
     7201 tgggtgtttt tctcttgcga cgcatatgtt gtggtttatt tgttctctta cgtttcataa
     7261 tcttattcct tctttatttt tgagatggtt ctatcttacc taaattaaat ctgaaaaaca
     7321 agccttcaac cgttctttta aataaactgt ctccactatg taatcttagg acttttctgt
     7381 cacacaatca atataaatgt catctgactg tcatattatt agataatact caatgaaaat
     7441 caaaaacaga ctcggagaca cagatgcaga tagaacaaaa gttcatcaaa tcaagtcaac
     7501 aacgtctgga ttttcgaaga ctataagtaa aaaataagag taaggcagta atccaacaat
     7561 tggctactaa ctcactctca tttctatttt cggattagtc aaaaacctac atttcttcag
     7621 gataaaccaa gccccattct ttacggagtt gggtcatgat ctccatgaca tcttgggtgt
     7681 aggctaggtg catggtgttt tcgatgcctg cgacagcttt ttccatatcc gccacttcat
     7741 aagaaagcgc cttggctgta tcgcccgctt cgattacctc ttggctaccg tcttcagtat
     7801 aggtaatgac tgccttctga ccacgaggat actcgtaaag ttcgatgtag cccttgtcgt
     7861 aggcaatagt accccgtttt ggctgtttgg catggagggt cagggcgatg gtcgccattt
     7921 cccccgcatc attgctgagc aaaatcccta cctgctcgtc cacacctgtc ggagctaatt
     7981 taacctgaga taggacttga tttggctttt cagtcataaa ccaacggaca aatgacagag
     8041 cgtagacacc aatatccaag agggcaccgc ctgccaagtt tttattgaaa aagcggttgg
     8101 tcatgtcata ttccttgtag ctgccaaagt tcatctgaat catcttcaaa tctccaagtt
     8161 ttccactggc aacaacttcg ctcaactgac gatagattgg catgtggaaa atggtcatag
     8221 cttctaccag gataacctga ttttcttctg ccagttgaat ggcttctgct aattcttctg
     8281 aattaagcgt aatagacttt tcacaaagga catgctttcc agccttcaag gcctttctca
     8341 aataattgat atgggtattg tgaggcgtag aaatatagat aatgtctact tcaggatcct
     8401 caaacacttc atcgatttct cgataaactt tctcgatgcc atatttttgc gcaaattcca
     8461 cacccttgtc ataggtacgg ttggctaccg aataaagatt tcctaccata gcctgcaagg
     8521 cctgaaccaa ttcattggca atgacacctg ttcccagggt cgcccattta tacttcattt
     8581 gcacttccat agtgtacctc actttcttct accattatag taaattccac ccttttctgc
     8641 acactctaac tagcatttgt gttacaataa gaacatgaat accgttgtac taaaggcatc
     8701 tgcccagcaa aaacaagcca tgatggctca ctatgaccgc tatcggcaga ccagcaaaaa
     8761 tccttacata gaagcatttt tcaaactgac cggtgccagc ctgtccatct acacttctgg
     8821 aaaagtcgtt tttcaggggg aaatggcaga gcaggaagca gggctctggg gctatgagcc
     8881 agaaagctct ggacagacga ctgtacctgg tcaaaacctg cccatgattg gtaccgatga
     8941 ggttggaaac gggtcttatt ttggaggttt ggcagtcgtt gctagttttg tccgcccaga
     9001 agaccatgcc tttctcaagt cgcttggtgt ggatgattcc aagaaaatga cagaccaaaa
     9061 aatctgccaa gtcgcccctc tcttaaaaga gaaaatcccc caccaagctc tgctattgtc
     9121 accgaaaaaa tacaacgaag tcatcgagca aggctacaat gcggtgtctg tcaaggtcgc
     9181 cctgcacaat caggctattt tcctactctt gcaaaagggt gtccagcctg agaagattgt
     9241 cattgacgct tttacttcca gtcaaaatta tcaaaagtac ttgaagaagg aagccaacca
     9301 atttgccaac cccgtcactc tggaagaaaa ggctgagggc aagtacctgg ctgtggcagt
     9361 atcttctatt attgctagag ctatgttttt agaaaatctt gtccagcttg gtcaactagt
     9421 tgggatgaat ctgccgtctg gtgcgggcag taaatccgac caagtcgcag caagcatctt
     9481 aaaacaatat ggtatggctg gtctcaacca aactgccaaa ctccactttg ccaatacaca
     9541 aaaagcacaa aaattattga aatgaggaaa aaatatggga gcaatccaaa aaacaaaacg
     9601 ctcaccacta gtcgccttct tagctgaatg gggtatcttt ttactattta tggcagcttt
     9661 ttttgccagt cgttacttta tctggaaccc tgtatctgtc gatggccatt ctatggaccc
     9721 aacgcttcaa caccaagaaa aactcatcat gctaaaaaca acctctatcg accgctttga
     9781 tattgttgta gccagtgaga cagacagcga cggcaaggaa aaactgattg tcaaacgtgt
     9841 gattggtatg ccaggtgaca ccattcgcta cgaaaacgat gtcctctatg ttaacgacca
     9901 aaaagtggac gagccttact tagatgaata tctagctgca ttccagaaag ataaacttca
     9961 agaagtctat tcttacaaca aacaattcca agctgtggcc caatctgctg aagctttcac
    10021 acaagatgcc aatggttacg tagactttac cgtgacagtc ccagaaggac aatactacct
    10081 gatgggagat gaccgccttg tctcactaga tagtcgtagt gtaggaacat tttcccgtga
    10141 aaacatcaaa ggagaagttg tctttcgcat gtggcctctc aaccgtattg ggactgttga
    10201 ttaatacaaa caagtttgga gcaatccgtt ccaaacttct ttttcattat tattggaact
    10261 aaactctatg aacgaagtgt attttaccgg tactattgac cggattattt ttgaaaatcc
    10321 tagtaatttt tataaaatcc ttctccttga aatcgaggaa actgacgctg actatgacga
    10381 ctacgaaatt attgtgaccg gaacgattgc cgatgtcatt gaaggagaag actatcgttt
    10441 ttatggcaat ctggtcaccc atcccaagta tggtcagcag ctgcaaattt cgcgttacga
    10501 acgcagcaag cctacctcag ctggtctggt taagtatttc tccagcgagc atttcaaggg
    10561 aattggacgc aaaacagctg aaaaaatcgt tgagctctat ggcgaagata ccattgataa
    10621 gattttagct gaacctgaaa aactaaccca aatcacaggc ctatccagca agaacatgca
    10681 ggcatttgtc gaaaaactcc gcctcaatta tggaaccgag ttgatcttgg caaaactagc
    10741 cgagtatggc attcccaata aattggcctt tcaaatccaa gaccagtata aggaaaaaac
    10801 acttcagatt attgaagaaa atccctatca actggtcgaa gatgtccagg ggctcggctt
    10861 taccattgcc gataggattg cggaaaacct gggaattgcc agtgattcac cccaacgttt
    10921 tcgggctggc atgctcttta gtctcattca tcgctccatg ga
//
LOCUS       DASFSW010000071        10958 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.071, whole genome shotgun sequence.
ACCESSION   DASFSW010000071 DASFSW010000000
VERSION     DASFSW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.071"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..291)
                     /locus_tag="U1099_001314"
     CDS             complement(<1..291)
                     /locus_tag="U1099_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867633.1"
                     /translation="MLTEEQLQDIFELADLMSNGDRELFLQLREVVFASDPHEILNFM
                     ERILDSESFDDFLDRVGESEKENLWLILIKLLEHLNYICVRDYKDNLEDFIYF"
     gene            complement(307..747)
                     /locus_tag="U1099_001315"
     CDS             complement(307..747)
                     /locus_tag="U1099_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867634.1"
                     /translation="MISYKRSIVIPSVISLVFILLGLGGLTRPEPSMGSVVYLIFLQA
                     LLVYLYILRKQKAVFILTKQYLEFQEHFWSERQRHSLEEIAEIRYIVSPVYRGYQSKR
                     LRIVGNKGGLLAVVSLNRLDGADFNDIYHFVEQVAPHIRWDFSN"
     gene            complement(749..2551)
                     /gene="pepF"
                     /locus_tag="U1099_001316"
     CDS             complement(749..2551)
                     /gene="pepF"
                     /locus_tag="U1099_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEM4867635.1"
                     /translation="MSKQRYEIEEKYQWDLTTIFPTDDAWEAELADLQAETEKTKEFA
                     GHLLDSAKSLLEISETQLSLMRRIEKLYVYASMKNDQDTREGKYQEFQAKAMGLYAAF
                     SQAFAFYEPEFMAITEEQLEAFKAEEPTLVQYSHQFEKLLAAKDHVLSQEVEEVLAAT
                     SEIFESPAETFSVLDNASLRFPEIADEDGHLVPLSHGNYIHFMESKNRDVRQEAYEAM
                     YGTYEQFQHTYAKTLQSNVKVNNLKARLRKYDSARHASLAANFIPESVYDTLVAAVNK
                     HLPLLHRYINLRKKLLGIDDLKMYDMYTPLSDFSTKISYEDALKKCQETLEIFGEDYS
                     AIVKEAFENRWIDVHVNEGKRSGAYSGGAYDTNAFMLLNWQDNLDNMYTLIHETGHSL
                     HSMLTRKTQPYVYGHYSLFLAEIASTTNENLLTEKLLAEVEDDKERFAILNHYLDGFR
                     GTVFRQTQFAEFEQAIYKADQEGQVLTADFLNELYGQTNEKYYGLSAEENPEIQFEWA
                     RIPHFYYNFYVYQYATGFAAASALANKIVNGSPEDKENYLNYLKAGSSDYSLNVIAKA
                     GVDMTKEDYLNDAFKVFEARLEELEALVEKGVHL"
     gene            complement(2783..3724)
                     /locus_tag="U1099_001317"
     CDS             complement(2783..3724)
                     /locus_tag="U1099_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEM4867636.1"
                     /translation="MLVALDEDGQVFNVLENPAPQGRYSCPGCGGLVRYKSGKVLRSH
                     FAHVTLRDCHYFSENESAQHLSLKSCLYSWLINAEQVELEKCLPSIGQVADLFVNNSL
                     ALEVQCSSLPISRLQLRTQAYHEAGLQVLWLLGKGLWIKERLSKLHKQFLSFSMNMGF
                     HLWELDDEKKELRLRYLIHEDLRGKVHCLTKVFPFGEGNLLDILRLPFAKQALSHLTF
                     PLDRDLPRYIAQQLYYRSPNWLTLQAESYSRGENLLTKTAEEWYPHIRLPRSAIGFAQ
                     IQKDLTLVYQDFDQYYGNIEDKQKQVLYPPIIYRKPM"
     gene            complement(3788..5845)
                     /gene="metG"
                     /locus_tag="U1099_001318"
     CDS             complement(3788..5845)
                     /gene="metG"
                     /locus_tag="U1099_001318"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEM4867637.1"
                     /translation="MGCYQEQGFICPELKGKSMTKTPFYITTPIYYPSGKLHIGSAYT
                     TIACDVLARYKRLMGHEVFYLTGLDEHGQKIEEKAKEAGLTPQAYVDGMAVGVKELWN
                     LLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEE
                     VFRDENGKVTGGIAPSGHEVAWVSEESYFLRLSKYQDKLVEFFNAHPDFIQPDGRMNE
                     IMKNFIEPGLEDLAVSRTSFTWGVPVPSNPKHVVYVWIDALLNYATALGYGQEETINY
                     DKFWNGTVYHMVGKDILRFHSIYWPIMLMMLDMKLPDLLVAHGWFVMKDGKMSKSKGN
                     VVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVA
                     MINKYFSGQVPTFVANVTDFDADLAAVVADNLASYHKYMEAVDYPRALEAVWNIISRT
                     NKYIDETAPWVLAKDEADRDKLAAVMAHLTAGLRVVAHLIQPFMMTTSNAIMEQLGLG
                     TAFDLENLDFAGLPAGLTVVAKGTPIFPRLDMEEEIAYIQAQMGGSSAISQEEEKEWN
                     PADVELKNEKAAIKFEDFDKVEIRVAEVKEVAKVEGSDKLLKFRLDAGDGEDRQILSG
                     IAKFYPNEQELVGKKVQIVANLKPRKMMGLLSQGMILSAEHGDNLILLTVDPSVPNGS
                     QIG"
     gene            5945..6538
                     /locus_tag="U1099_001319"
     CDS             5945..6538
                     /locus_tag="U1099_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEM4867638.1"
                     /translation="MFTKKNILTHLFFLIIAVGLKRYGVTAPDLTWNMFLALVALDFA
                     ILTNHTKSHILKVIAGLLWLFFYPNTFYMVTDIVHMHFANTVLWQRESMILFMLYVPS
                     IFFGVMAGVESVRLIFSAFSIKSYWLKLFMISGLSLLSSFAIHIGRYARLNSWDIFTR
                     PTVVIDEMLAVISWDALPFILGFTFIQIMVLVFSINE"
     gene            6839..7876
                     /locus_tag="U1099_001320"
     CDS             6839..7876
                     /locus_tag="U1099_001320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEM4867639.1"
                     /translation="MKEGDLLFTPISEKIDINQVREHSKLSPETLAKKQARDRELEAI
                     LKGEDNRLLLVIGPCSSDNEEAVLDYAHRLAKLQEEVKDKIFMVMRVYTAKPRTNGDG
                     YKGLMHQPDTDAAPSLINGIKAVRNLHYRVITETGLTTADEMLYPENLPLVDDLVSYI
                     AIGARSVENQQHRFVASGIDVPTGLKNPTSGNLKVMFNGLFAAQKKQSFLFNNTEVET
                     SGNPLTHVILRGAVNENGKYLPNYYYDNLLETIDLYDQFGLKNPFIIIDTNHDNSGKN
                     YLEQIRIVRQTLINRDWNESIRNYVRGFMIESYIEDGRQDNPDVYGKSITDPCLGWEK
                     TQELIREIYNR"
     gene            7886..8911
                     /locus_tag="U1099_001321"
     CDS             7886..8911
                     /locus_tag="U1099_001321"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEM4867640.1"
                     /translation="MGIHKRSTSLDIDKVKELSKLEGEFLAAKNRRDEELKRIIRGED
                     DRLLLVIGPCSSDNEEAVLEYARRLSKLQEEVKDKIFMVMRVYTAKPRTNGEGYKGLV
                     HQPDTSKLPDLINGIAAVRNLHYRVITETGLTTADEMLYSANYPLVEDLVSYHAIGAR
                     SVEDQEHRFVASGVDMPTGMKNPTSGNLTVMFNGIYAAQNKQNFIYRDAEVDTDGNPL
                     AHAILRGANTEKGGYEPNYYYDILLKTIKQYEQFGLKNPFIVIDTNHDNSGKTYLEQI
                     RIVRQTLINRAWNESIRKYVRGFMIESYLEDGRQDSPEVFGKSITDPCLGWKKTEALI
                     REIHQTV"
     gene            8957..9826
                     /locus_tag="U1099_001322"
     CDS             8957..9826
                     /locus_tag="U1099_001322"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEM4867641.1"
                     /translation="MNIDGYTRLAAVVAKPIKHSISPFIHNLAFKETGVNGVYVAWEI
                     PEEDLAVTLENIKRYDMFGINLSMPYKQAVIPYLDDLTASARLIGAVNTVIHKDGKLI
                     GHNTDGIGFFKSLERLKGFQVKNKRLTILGGGGASTAIIAQATLDGAKEITIFCRQQS
                     LERTQASLTPIAQATGVPMQVLALDDSQLLQEHITNSDLLVNGTSVGMDGYSQPVPST
                     IRFPENLLVADVIYQPFETPLLKLAQSQGNPTINGLGMLLFQAAEAFQAWTGKEMPTD
                     LIWDQLVQKYDIK"
     gene            9839..10906
                     /locus_tag="U1099_001323"
     CDS             9839..10906
                     /locus_tag="U1099_001323"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEM4867642.1"
                     /translation="MKLTVNLPNTPYDILIQRGSLAQTGTWIKELWKPQKIAIITDDH
                     VGSLYRETVQSSLEQAGFETIVFEFPEGEASKNLDTVNQAYEFLVKNGMTRSDGIIAL
                     GGGVVGDLAGFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFCQP
                     DGVLIDPDTLKTLGKRELIEGMGEVVKYGLIDDVELWETLDQLDGSVESILEHADYII
                     YHSCEVKRKVVVEDELDNGVRLYLNFGHTIGHAIEVTAGYGQVMHGEAVAIGMVQISR
                     AAEKKGLMPAGMTEKIIAMCEKFGLPTTHQPWNVDELYATLTHDKKARGKTIKLVIVP
                     QLGQAAIHQIPMEEMLEFLQV"
BASE COUNT         3195 a         2459 c         2190 g         3114 t
ORIGIN      
        1 aaagtagata aaatcttcaa gattgtcctt ataatcacgc acacaaatat agttcaaatg
       61 ctctagtaat tttatcaaga ttaaccaaag attttccttt tcagactctc caacacgatc
      121 caagaaatca tcgaatgact ctgaatctaa aatacgttcc ataaaattga gaatctcgtg
      181 tggatcactg gcgaagacta cttctctaag ttggagaaat agttccctat caccatttga
      241 cattaaatct gctaattcaa aaatatcttg tagttgttct tctgttaaca tagtcccctc
      301 tctttattaa tttgaaaaat cccatcttat atgcggtgct acttgctcta caaaatggta
      361 aatatcatta aaatctgcac catcaagcct attcaagctg acgactgcaa gtagtccccc
      421 cttatttcct acaattcgca aacgtttcga ctgatagcca cgatagacag gagaaacgat
      481 gtatcgaatc tctgcaatct cttctaacga atggcgttga cgttcactcc aaaagtgttc
      541 ttgaaattct aggtactgtt ttgtcaagat aaaaactgcc ttttgctttc tcaatatata
      601 taaataaact agcaaagctt gtaaaaatat caaatagaca acacttccca tagatggctc
      661 aggtctagtc aaaccaccaa gaccgagcaa gatgaaaaca agactaatca cactgggaat
      721 cacaatcgaa cgtttatagg aaatcatatt agaggtgcac acctttctca acgagagctt
      781 ctaattcttc caaacgagcc tcaaaaacct taaaggcgtc gttgaggtaa tcttccttgg
      841 tcatgtctac acctgcttta gcaatgacat tgagggaata atctgagcta ccagccttca
      901 agtagttgag atagttttct ttgtcttctg gactgccgtt gacaatcttg tttgcaagag
      961 ctgatgcagc cgcaaaacct gtcgcatatt gatagacata gaaattgtaa tagaagtgtg
     1021 gaatacgagc ccactcaaac tggatttctg gattttcttc ggcagaaagt ccgtagtatt
     1081 tctcgtttgt ctgaccgtac aattcgttga ggaaatcagc cgtcaagact tgaccttcct
     1141 ggtctgcctt gtagatagct tgttcaaact cggcaaattg tgtctggcgg aagactgtgc
     1201 cacggaaacc atctaggtaa tggttaagaa tagcaaagcg ttccttgtca tcttctactt
     1261 ctgccaagag tttttctgtt aggagatttt cattggttgt tgaagcaatt tctgccaaga
     1321 agagggaata atgaccatag acatagggtt gagtcttacg agtcaacatg gaatgaagag
     1381 aatgccctgt ttcatggata agggtgtaca tgttatccaa attatcctgc cagttgagaa
     1441 gcatgaaggc gttggtgtcg taggcacctc ctgaatatgc tcctgagcgt tttccttcat
     1501 taacatggac atcaatccag cgattttcga aggcttcctt cacaatagcc gaataatcct
     1561 caccaaagat ttctaaggtt tcttggcatt tcttgagtgc atcttcataa gaaatcttgg
     1621 tgctgaaatc tgacaatggt gtgtacatat cgtacatctt caagtcatca atacctagga
     1681 gtttcttacg caaattgata taacggtgca agagcggaag gtgcttgttg actgccgcca
     1741 ctaatgtatc gtaaacagat tctggaatga aattagctgc aagtgatgcg tggcgagctg
     1801 agtcatactt ccgtaaacga gctttcaggt tattgacttt gacattagac tgaagggtct
     1861 tagcgtatgt atgctggaac tgctcgtagg taccgtacat agcttcgtat gcttcttggc
     1921 ggacatcacg gtttttagac tccataaagt ggatgtagtt tccgtgtgaa agtggcacca
     1981 agtgaccgtc ttcatctgcg atttctggga aacgcaggct agcattgtcc aagactgaga
     2041 aggtttcagc aggtgattca aaaatctcgc tggttgccgc aaggacttct tccacctctt
     2101 gtgacaggac atggtccttg gctgccagaa gtttttcaaa ttggtggctg tactgaacaa
     2161 gagtaggctc ttctgcctta aaggcctcca gctgctcttc agtaatggcc ataaattcag
     2221 gctcatagaa ggcaaaagcc tgtgaaaagg cagcatacaa gcccatggcc ttagcttgaa
     2281 attcctgata ttttccttcg cgagtatctt ggtcgttctt catagacgca tagacataga
     2341 gtttttcaat gcgacgcata agactaagtt gcgtttcgct aatttctaag agacttttag
     2401 ctgagtccag taaatgacct gcaaattctt ttgttttttc agtttcagct tgtaaatccg
     2461 caagttctgc ttcccaggca tcatcggttg ggaaaatagt agtcaaatcc cactggtatt
     2521 tttcttcaat ttcgtaacgt tgtttagaca taattaatcc tccgtattct ttattctaac
     2581 ataaaaaatc ccctctttcc aacggaaagt gaggacttat aggttactat ttcaactgga
     2641 aaatagtttt tttggtattc gctatcgcaa gtcgtcacta ggctaacaaa gtctacaacc
     2701 ttcaccgaca gtagtatgac taacataaaa tattgagcat attttcaaaa gtaatatata
     2761 cacctccatc agctatctct atttacattg gttttctata aattataggt ggatatagaa
     2821 cctgtttctg cttgtcttca atattgccgt aatactggtc aaaatcctga taaactaagg
     2881 ttaaatcttt ttgtatttgg gcaaagccaa tagcagagcg gggtagacgg atatgtgggt
     2941 accattcttc tgccgtcttt gtcagcaaat tctcccctcg gctataagac tctgcctgca
     3001 gggttaacca attgggggat ctgtaataaa gctgttgagc aatgtagcga ggcaaatccc
     3061 tatctaacgg gaaagtaaga tgcgataaag cttgcttggc aaaaggcaga cgcaagatgt
     3121 ccaataaatt tccctcgcca aatggaaaaa cctttgtcag acagtggacc ttacctcgta
     3181 agtcttcgtg aataaggtaa cgaagacgca attctttttt ctcgtcatcc aattcccaga
     3241 ggtgaaaacc catgttcata ctaaaagaga gaaattgctt gtgtaattta cttaatcttt
     3301 cttttatcca taggcctttt cccaacagcc agagaacctg aagaccagct tcatgatagg
     3361 cttgcgtcct cagctgcaaa cgagaaatgg gcagactgga acattggact tccaaagcta
     3421 gactattatt aacaaataaa tcagcgacct gccctatact aggcaggcat ttttcaagtt
     3481 caacttgttc ggcattgatt aaccaactgt acagacaaga cttaagtgac agatgctggg
     3541 ctgactcatt ctcggaaaaa tagtggcagt cccgcagggt tacatgggca aaatgcgaac
     3601 gcagaacttt tccggatttg tatcggacca gccctccaca acctggacaa gaatagcgac
     3661 cttgcggcgc aggattttcc aagacattaa aaacttgtcc atcttcatca agtgcaacta
     3721 acataaaacc tcctctcttt attagttcgc aaaaaaacac ccagctatgc tgagtgtttg
     3781 aattttctta cccaatctga ctgccatttg gcacagatgg atcgactgtt aagagtatta
     3841 aattgtcacc atgttcggct gagaggatca tgccctgact gagcaagccc atcatcttac
     3901 gtggcttgag gttggcaacg atttggactt tcttgccgac caactcctgc tcgttcggat
     3961 agaacttggc gataccagac aggatttggc ggtcttcgcc atcacctgcg tccaggcgga
     4021 atttgagcag cttgtcagaa ccctccactt tggctacttc cttgacttcg gccacacgga
     4081 tttctacctt gtcgaagtcc tcgaacttga tcgcagcttt ttcgtttttg agttccacgt
     4141 cagctgggtt ccactctttt tcttcctctt gggaaatagc agagctgcca cccatttgag
     4201 cttggatata ggcgatttcc tcttccatat ccagacgtgg gaagataggc gttccttttg
     4261 cgaccactgt caaacctgct ggaagacctg caaagtcaag attttcaagg tcaaatgctg
     4321 ttcccaaacc aagttgttcc ataatagcat tagacgtcgt catcataaat ggttgaatca
     4381 aatgtgccac cacacgaaga cctgctgtca agtgagccat caccgctgcc aacttgtcac
     4441 ggtccgcttc gtccttagcc aagacccaag gtgcagtttc atcaatgtac ttgttggtac
     4501 gggagatgat attccaaaca gcttccagag cgcgtggata gtcaacagct tccatatact
     4561 tgtggtagct tgccaagttg tcagctacta ctgccgccaa gtccgcatca aaatcagtca
     4621 cgttagcaac aaaggttggt acctgaccgc tgaaatactt attaatcata gcaaccgttc
     4681 ggttgagcaa gtttccaagg tcattggcta gttcgtagtt gatacggcca acgtagtctt
     4741 ctggcgtgaa ggttccgtca gagccaactg gcagactacg catgaggtaa tagcggagcg
     4801 gatccaaacc aaaacgctcg accagcattt ctggatagac cacattgccc ttagacttgg
     4861 acatcttgcc atctttcatg acaaaccagc catgagcaac caagaggtct ggcaacttca
     4921 tatccaacat catgagcatg attggccagt aaatggagtg gaaacgaaga atgtccttgc
     4981 caaccatgtg gtaaactgtt ccattccaga acttgtcgta attaattgtt tcttcctgac
     5041 cataacccaa ggcagttgcg tagttgagca gggcatcaat ccagacatag acaacgtgtt
     5101 taggattgga cggaacaggc acgccccaag taaatgaggt acgggacact gccaaatctt
     5161 ccaagcctgg ctcgatgaag tttttcatga tttcattcat acgaccatct ggctggataa
     5221 agtctgggtg agcattgaag aactcgacca acttatcctg atacttgctg aggcgaagga
     5281 agtaggattc ttctgaaacc caggcaacct cgtggccgct cggagcgata ccgcctgtta
     5341 ccttgccgtt ttcatctcgg aagacctctt ctaattggct ctcggtgaag aattcctcgt
     5401 ccgatacgga gtaccaacca gaatactctc ccaagtaaat gtcgtcctgc gccagcaatt
     5461 tttcaaaaac agccgccacc acttcctcgt ggtaatcgtc ggttgtgcgg atgaacttgt
     5521 cgtacgagat gtcgagcaag ttccagagct ctttaacgcc aaccgccatg ccgtcaacgt
     5581 aggcttgagg tgtcaaacca gcttcttttg ccttctcctc aatcttctgc ccatgctcat
     5641 caagccctgt cagatagaag acctcgtggc ccatgagacg cttgtagcga gccaagacat
     5701 cacaggcaat ggtcgtgtag gctgaaccga tgtgaagttt tccagacgga taataaatcg
     5761 gtgtggtgat ataaaacggt gtttttgtca tactttttcc tttcaactca gggcaaatga
     5821 aaccctgttc ttgataacac cccattttat catattttca gctaaatcac aaaatggttc
     5881 taactacaat tcttcctcta tctatgctat actatgatac aactcaaaca gaaagaggtc
     5941 atctatgttt acaaagaaaa atattcttac tcatctcttt ttcctcatca tagcagtcgg
     6001 actaaaacgg tacggtgtca cagcacctga cttgacatgg aatatgttct tggcattggt
     6061 agcacttgat tttgcaatcc ttaccaatca tacaaagtcc catatcctca aagtgatagc
     6121 aggtctcctc tggctctttt tctaccccaa tactttttac atggtgacag acatcgtcca
     6181 tatgcacttt gccaacacag tactatggca acgagaaagc atgattctat ttatgcttta
     6241 tgttccaagt attttctttg gggtcatggc tggcgttgaa agtgtacgac ttattttctc
     6301 tgctttttct atcaaatcct actggctaaa actgtttatg attagtggtc tttccctact
     6361 atccagcttt gccattcata tcggacgata tgcaagactg aattcatggg acatctttac
     6421 cagaccaact gtcgttattg atgaaatgct tgcagttatt tcgtgggatg ctctcccctt
     6481 tatcctcggc ttcaccttta tccaaattat ggttcttgtc ttttcaataa atgaataaga
     6541 tttttatctc atagccatta tcttcaagca tcttcctgca ctcaaggact atcgttcctt
     6601 ttgggaagat ttttcaagga cataaacaaa attttttgat aaatttatat cttctccgtt
     6661 tctctccatt acatttttat ccaaactgta tgaggcaaaa atcgttaacc tattgtaagt
     6721 aaaatttctt aaatttcctt atatataaaa tattcagaat ttactgtaaa ttctacataa
     6781 aatcatggat attgatttta aattcttaaa aataatggta taattgagga acctataaat
     6841 gaaagaggga gatctattgt ttacaccaat cagcgaaaaa attgacatca atcaagtacg
     6901 agaacattca aaactttctc cagaaacact tgctaaaaaa caagctcgtg accgtgaact
     6961 tgaagccatc ttaaaaggtg aagataatcg tcttctccta gtaattggac catgttcttc
     7021 tgacaatgaa gaggctgtct tagattatgc tcaccgcttg gccaagctcc aagaagaggt
     7081 caaagataaa atttttatgg tcatgcgcgt ctatactgcc aagccacgta ccaatggtga
     7141 tggttataaa ggactcatgc accaaccaga tacagatgct gcaccaagcc tcatcaacgg
     7201 aatcaaagca gttcgtaacc tccactatcg tgtcattacg gaaacaggtt taactactgc
     7261 tgacgagatg ctctaccctg aaaacctgcc tttggttgat gacttggttt cctacatcgc
     7321 tatcggagct cgttcggttg aaaatcagca gcaccgtttt gtagcgtcag gcatcgatgt
     7381 tccaacaggc ttgaaaaatc caacatctgg taacctcaaa gtcatgttca acggtctttt
     7441 tgctgctcag aagaagcaat ccttcctctt caacaatact gaagttgaaa catctggcaa
     7501 cccacttact cacgttattc ttcgtggcgc agtcaatgaa aatggtaaat acctaccaaa
     7561 ctattactat gacaatcttc tagaaaccat tgacctctat gatcaatttg gtttgaaaaa
     7621 tccatttatt attatcgata ccaaccatga caattctggg aaaaattatt tggaacaaat
     7681 ccgtatcgtc cgtcaaacct taatcaaccg tgactggaac gaatccattc gtaactacgt
     7741 tcgcggattc atgattgaat cctacattga agacggtcgc caagacaatc cagatgtcta
     7801 tggaaaatcc atcacagacc cttgcttagg ctgggaaaaa acacaagaac tcatccgaga
     7861 aatttacaac agataggaag atattatggg aattcacaaa cgtagcacca gtttagacat
     7921 cgataaagta aaggaacttt ccaagttaga aggagaattt ctagctgcaa aaaatcggcg
     7981 cgatgaagaa ctgaaaagaa ttatccgagg cgaagatgat cgtctgctct tggttatcgg
     8041 tccttgctca tcagataatg aagaggctgt tctagaatat gcacgtcgtc tctctaagct
     8101 tcaagaggaa gtcaaagata aaatcttcat ggtcatgcgg gtttacacag ctaagccacg
     8161 taccaatggg gaaggctata aaggactggt tcatcagcca gatacttcta aattaccaga
     8221 cctcattaac ggaattgcag ccgttcgtaa tttgcattac cgtgtcatta ctgagaccgg
     8281 actcacaact gctgatgaaa tgctctactc tgccaactat ccactggtgg aagacttggt
     8341 gtcctaccat gctatcggag cacgttcggt tgaagaccaa gaacatagat ttgttgcatc
     8401 aggtgtcgat atgccaactg gtatgaaaaa cccaacttct ggtaatttga ccgttatgtt
     8461 caatggtatc tacgccgcac aaaacaaaca gaactttatt taccgtgatg cagaagttga
     8521 tacagatggc aaccctctag ctcatgctat ccttcgtggt gccaatactg aaaagggcgg
     8581 ttatgagcca aactactact atgatattct cttgaaaacc atcaaacagt atgaacaatt
     8641 tggtcttaaa aatccattta tcgtaattga taccaaccac gacaattctg gtaaaactta
     8701 cctggaacaa atccgtatcg tccgtcaaac cctcatcaac cgagcttgga acgaatctat
     8761 tcgcaaatat gtccgtggtt tcatgattga atcctactta gaagacggcc gtcaagatag
     8821 ccctgaggta ttcggcaaat ccattaccga cccttgctta ggttggaaaa aaacggaagc
     8881 tctgattcgt gaaattcatc aaaccgtcta attctttcct taatttttta tacgataaat
     8941 aatttggagg taagctatga acattgatgg ttacactcgt cttgctgccg ttgttgcaaa
     9001 gccaatcaaa cactccatct cccccttcat tcataatctg gcttttaagg aaacaggtgt
     9061 aaatggtgtc tatgtcgctt gggaaattcc cgaggaagat ttggctgtaa cgcttgaaaa
     9121 tatcaaacga tatgatatgt ttggtatcaa cctttccatg ccctataaac aagctgtcat
     9181 tccttatcta gatgatttaa cagcctctgc acgcctgatt ggcgcagtca ataccgtgat
     9241 tcataaagat ggaaaactaa tcggtcacaa caccgatgga attggatttt tcaagagttt
     9301 ggaaagacta aaaggctttc aagttaagaa caagcgacta accatcctag gtggtggtgg
     9361 tgcttctact gctatcatag ctcaggctac attggatggt gcaaaggaaa ttaccatttt
     9421 ctgccgtcaa caaagcttgg aaagaacaca ggcgagtctt acacctattg ctcaagcaac
     9481 tggagttccc atgcaggtcc tagcgcttga tgatagccaa ttactacagg aacatatcac
     9541 caactccgac ttactagtca acggaaccag tgtaggcatg gatgggtatt ctcaacctgt
     9601 tccttctacc attcgctttc ccgaaaatct tttggttgcg gatgtgattt atcaaccatt
     9661 cgaaacacct ttattaaaac tagcccaatc acaaggcaac cctactatta acggtttggg
     9721 catgctcctt ttccaagcgg ctgaagcatt ccaagcctgg actggtaagg aaatgccgac
     9781 tgacttgatt tgggaccaat tagtccagaa atacgacatc aaatagagag gaaatctgat
     9841 gaaactgacc gtcaatcttc ccaacactcc ctacgacatc cttatccaaa gaggaagttt
     9901 agcacagaca ggtacatgga tcaaagaact ttggaaacct cagaaaatcg ccattattac
     9961 agatgaccac gttggctcac tctatcgcga aacagttcag tcaagcttag aacaagctgg
    10021 ttttgaaacc attgtttttg aatttccaga aggtgaagcc tcaaaaaatc ttgataccgt
    10081 caaccaggcc tacgaatttc tcgtaaaaaa tggtatgaca cgaagtgatg gaatcattgc
    10141 tctcggcggg ggagtcgttg gcgatttggc aggttttgtc gcttctacct atatgcgagg
    10201 tatccatttc ctacaaatcc caaccagcct aactgcccaa gtcgattctt ctatcggggg
    10261 aaaaactggc gtgaacaccc catttgccaa aaatatggtg gggaccttct gccagccaga
    10321 cggcgttctc attgaccctg ataccctaaa aacactcggc aaacgagaat tgattgaagg
    10381 aatgggcgag gttgtcaaat atggcttaat agatgatgtg gagctctggg aaaccctaga
    10441 ccaattagac ggctctgtcg agagcattct ggagcatgct gactatatca tctaccactc
    10501 ctgtgaagtc aaacgcaagg ttgttgtcga agatgaatta gacaacggtg tcagactcta
    10561 cctcaacttc ggtcacacga ttggacatgc tattgaagtg accgctggct acggccaggt
    10621 catgcacggt gaagctgttg ccattggtat ggtacaaatc tcacgcgcag ctgagaaaaa
    10681 aggactgatg ccagctggta tgacagaaaa gattatcgcc atgtgtgaaa aattcggctt
    10741 gccgactaca caccagcctt ggaatgtaga tgaattatat gccaccctaa ctcacgataa
    10801 aaaagcacgt ggcaagacca tcaaacttgt catcgttcct caattgggac aggctgccat
    10861 ccatcaaatt ccaatggaag aaatgctaga atttctacaa gtatagtcat ttagttgact
    10921 tttacgtcga agtaataaaa gtcaactaat actcaatg
//
LOCUS       DASFSW010000072        10485 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.072, whole genome shotgun sequence.
ACCESSION   DASFSW010000072 DASFSW010000000
VERSION     DASFSW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.072"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            16..582
                     /locus_tag="U1099_001324"
     CDS             16..582
                     /locus_tag="U1099_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEM4867643.1"
                     /translation="MKNHLEIEYKTLLTKSEYKRLLTDFSDVSPILQTNHYIDTPDLD
                     MKNHRFSLRIRTFEDSAELTLKIPQEVGNQEYNQVLDIQTAHDLLENFQLPAGQISDI
                     ISATEIPLDKLAVWGILTTKRYEKQTSIGLMALDENDYVGQKDYELEVEVTDADAGKI
                     LFDEFLKKKSIQFKYASSKVARTAAHMK"
     gene            727..1704
                     /locus_tag="U1099_001325"
     CDS             727..1704
                     /locus_tag="U1099_001325"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM4867644.1"
                     /translation="MVQPLGEHNIKLFSLNSNRDIAEKIADSAGIPLGKLSSRQFSDG
                     EIQINIEESVRGVDVYIIQSTSYPVNNHLWELLIMIDACKRASANTITAVIPYFGYAR
                     QDRTASPREPITAKLVANMLVKAGVDRVLTLDLHASQIQGFFDIPVDNLLTEPLFAAY
                     YMEKGLCGEDVVIVSPKNSGIKRARNIAEFLNAPIAIIDYAQDDSERSEGYIIGDVAG
                     KKAILVDDILNTGRTFSQASKIVQEGGATEIYAVASHGLFAGTATQLLDEAPIKEILV
                     TDSVASKEQHPKNIVFLTASDLIADAIHRIQEHQPLSPLFKFTSPEKEN"
     gene            1715..2830
                     /locus_tag="U1099_001326"
     CDS             1715..2830
                     /locus_tag="U1099_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM4867645.1"
                     /translation="MIYLDNAATTALSPTALQRMMEVAQENYGNPSSIHQSGRKANQR
                     LRQSRQEIAQILSASPEQIIFTSGGSEADTLAIQGYALAHQSKGKHLITTAIEHHAVL
                     HTMQYLEERFGFEVTYIQPVDQVITAQQIQDALRPDTILVSVMFANNETGQLLPIKEI
                     GELLADHQAVFHVDAVQAIGKIAISPSNYGIDLLAASAHKFHGPKGTGFLYSKVHKFD
                     SLIHGGKQEQQHRAGTENLPAIAAMATALTEQYESLDTHHQHVSELRQHIIEGLSGLD
                     YYLNQAGPHLPHVLNIGFPGKLNEQLLMQLDLAGIAVSSGSACTAGVVQNSHVLEAMY
                     GKDSHRLKESIRISLSEINTKEEIDTLIIELKKILGG"
     gene            2832..3176
                     /locus_tag="U1099_001327"
     CDS             2832..3176
                     /locus_tag="U1099_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEM4867646.1"
                     /translation="MAFQQSVTLKDCNFTYHISPAIKKYTLRDNTFEQTKAGHYQLTR
                     LLEEIPNSNQGFLLKIIINQDLTGFKINITDKSGLHLVNIFKPESNKVIQEKFYFLMD
                     SLIDRDIFTKES"
     gene            3178..3381
                     /locus_tag="U1099_001328"
     CDS             3178..3381
                     /locus_tag="U1099_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEM4867647.1"
                     /translation="MIEIHCLDAYKQERIQTFENKDATILAFSGCLTLPDYYPVTSIT
                     QDGQALDYKGTIGDLYRYLQTLD"
     gene            3516..4154
                     /locus_tag="U1099_001329"
     CDS             3516..4154
                     /locus_tag="U1099_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEM4867648.1"
                     /translation="MKNDKKSDIPRATAKRLSLYYRIFKRFYAGNIEKASSKEIAEAI
                     GIDSATVRRDFSYFGELGRRGFGYNVKELMDFFADILNDTSITNVMLVGVGNMGRALL
                     HYRFHERNKMKIVMAFEADDNPAVGTTDENIPIHAISEIKERISEANSQTAILTVPSV
                     KAQEVTDILVEAGVKGILSFSPVNLSVPKDVVVQYVDLTSELQTLLYFMRKG"
     gene            4158..4850
                     /locus_tag="U1099_001330"
     CDS             4158..4850
                     /locus_tag="U1099_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEM4867649.1"
                     /translation="MKKPVIGISGNEYKTGDHTEPLLSYTQTCLVQAIEDAGGLPLIL
                     PVTEPDLAKYYIHLIDKLILTGGQNVQPSYYREERTIDSDNYLPKRDEFELAIIRAAQ
                     ENQKPIFGICRGLQLYNVAQGGSLHQSISEHWQDIDGQEVSQTIQLTQNSPLYDIYES
                     DPSVNSFHRQAIKDLAPDLEIIALSDNQQIIEAVHSAYPTKFLGVQWHPELLYGKREI
                     EKELFHYIVNKL"
     gene            complement(4847..5527)
                     /gene="radC"
                     /locus_tag="U1099_001331"
     CDS             complement(4847..5527)
                     /gene="radC"
                     /locus_tag="U1099_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEM4867650.1"
                     /translation="MYQIEFKEEAILPRERLVEVGAERLSNQELLAIFIRTGTKKEPV
                     SILSNKLLNRLESLAALRELSIEELQSLTGIGRVKAIEIKAMIELGKRINQSELLLNE
                     RILGSEKLGRKMIHEIGHKKQEHLVALYLNTQNQIISQKTIFIGSVNRSIAEPREILH
                     YAVKCMATSIIIVHNHPSGSVQPSRNDLLFTENLKESCEKLGLVLLDHLIVGNKDYYS
                     FREESELF"
     gene            complement(5641..6390)
                     /locus_tag="U1099_001332"
     CDS             complement(5641..6390)
                     /locus_tag="U1099_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEM4867651.1"
                     /translation="MSKREKKKKRKGSFWRNFLTVVLILISLALIFNTSIRNFIIGWN
                     TNKYQISNVTTEDIEKNKQAETTFDFEQVQSISTEAILAAQWDAQRLPVIGGIAVPEL
                     GINLPIFKGVFNTSLMYGAGTMKENQEMGKGNYALASHHIFGVTGAADVLFSPLDRAK
                     NGMKIYITDKTNVYTYVIDSVEIVSPESVYVIDDVEGRTEVTLVTCTDYYATQRIVVK
                     GVLESTTPYNETAKDILDSFNKSYNQYDYGQ"
     gene            complement(6397..8841)
                     /gene="gyrA"
                     /locus_tag="U1099_001333"
     CDS             complement(6397..8841)
                     /gene="gyrA"
                     /locus_tag="U1099_001333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEM4867652.1"
                     /translation="MQDKNLVTVNLTNEMKSSFIDYAMSVIVSRALPDVRDGLKPVHR
                     RILYGMNELGITPDKPHKKSARITGDVMGKYHPHGDSAIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVNFADNYDASEREPEVLPAR
                     FPNLLVNGTTGIAVGMATNIPPHNLGETIEAVKLVMDNPEVTTREIMEVLPGPDFPTG
                     ALVMGKSGIHRAYETGKGSIVLRSRTEIEEYGNGRERIVVTEFPYMVNKSKVQEHIVK
                     LVQEKRIEGITAVRDESNREGVRFVIEVRRDASANVILNNLFKQTQLQTNFSFNMLAI
                     QNGVPKILSVRQILESYIEHQKEVVTRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRNSETDAIAQAELMEKFDLSVRQSQAILDMRLRRLTGLERDKIQSEYDELVALIAD
                     LADILAKPERVVQIIKDELEEVKRKYADPRRTELMVGEVLSLEDEDLIEETDVLITLS
                     NQGYIKRLAQDEFQAQKRGGRGVQGTGVKDDDFVRELVSTSTHDRLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLEENEAIQTIINVTKDQEADSYLFFATRQGVVKRTS
                     VSEFANIRQSGLKALNLKEDDELINVFLTNGQADIIMGTKFGYSVRFTETDVRNMGRT
                     ATGVRGINLREGDQLVGATMISDDQEVLVLTEKGFGKRTPASEYPTKGRGGKGIKTLK
                     VADKNGSLAGLTTVSGDEDIMVITDTGVIIRTSVANISQTGRSTMGVKVMRLNDEAKI
                     MTFALVDAAEIKEEKE"
     gene            9030..10013
                     /locus_tag="U1099_001334"
     CDS             9030..10013
                     /locus_tag="U1099_001334"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEM4867653.1"
                     /translation="MTATKQHKKVILVGDGAVGSAYAYALVNQGIGQELGIIDINKDR
                     TQGDAEDLSHALAFTFPKKIYSAEYSDAHDADLVVLTAGLPQKPGETRLELVEKNLRI
                     NQQIVTEIVNSGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AEKIGIDARSVHAYIMGEHGDSEFAVWSHANVAGVKLYDWLQDNRDIDEQGLVDLFVS
                     VRDAAYSIINKKGATYYGIGVALARITKAIFDDENAVLPLSVYQAGQYEGVEDVFIGQ
                     PAIIGAHGIVRPVNIPLSDAELQKMQASAKQLKDIIDDAFANPEIAAGVKN"
     gene            complement(10056..10289)
                     /locus_tag="U1099_001335"
     CDS             complement(10056..10289)
                     /locus_tag="U1099_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEM4867654.1"
                     /translation="MELRSREGGDIWLFGGASLVQDCLEAGVIDHLIIGIIPTILGDG
                     IPLFASLLEEKKLLLVESTVTDGIAMLRYDIRR"
BASE COUNT         3212 a         2343 c         1878 g         3052 t
ORIGIN      
        1 gaaagggttt actgcatgaa aaaccacctt gaaattgagt ataaaacctt actgaccaaa
       61 tccgaataca aacgcctgct tactgacttt tcagatgtga gcccaattct tcaaacaaac
      121 cactacattg ataccccaga cctagatatg aagaatcacc gcttttcact ccgtattcgt
      181 acatttgagg actcagcaga actaacctta aaaattccac aagaagtcgg caatcaagaa
      241 tataaccaag ttctagacat ccaaactgct catgatttac tggaaaattt tcaactacca
      301 gcaggacaaa tttctgatat tatttctgca accgaaattc cacttgataa acttgctgtc
      361 tggggaatcc tcacgacaaa acgctatgag aaacagacat ctataggcct tatggccctc
      421 gatgaaaatg attatgtggg acaaaaagat tacgaattag aagtcgaagt gaccgatgca
      481 gatgcaggga aaattctttt tgatgaattc cttaagaaga aatcaattca attcaaatat
      541 gctagtagta aggtcgcacg aactgctgcc cacatgaaat aactataaaa aatcagccaa
      601 ctaaggttga ttttttagtt tcactactga aaatttttca tttttttgat agaatagaac
      661 tatctatcgt aaaaaatact tttatgtcat cataaaagct cagtgtgtaa aaggagttac
      721 tttaaaatgg tacaaccact cggtgaacat aacattaaat tattctcact caatagcaac
      781 cgtgatattg ctgaaaaaat tgctgattct gctggaatcc ctcttggaaa gctgtcttct
      841 cgtcagtttt ctgatggtga gattcaaatt aacattgaag aatctgtacg tggcgtagac
      901 gtctacatta tccaatcaac aagctatccg gtcaataatc acttgtggga attattgatt
      961 atgatcgatg cttgtaaacg tgctagtgcc aacaccatta cagctgtcat cccttatttc
     1021 ggctatgctc gtcaagaccg taccgcttct ccacgcgaac caattacagc taaattagtt
     1081 gcaaatatgc tggttaaagc tggtgtcgac cgtgtattga ctcttgactt gcacgcttca
     1141 caaattcaag gtttctttga tattccagta gacaatctcc ttacagaacc actttttgca
     1201 gcctactaca tggaaaaagg actctgcggt gaagatgtcg tcattgtgag tcctaaaaat
     1261 tctggtatca aacgcgcgcg taatatcgct gaatttctaa atgcaccgat tgcaattatc
     1321 gactacgctc aagatgattc tgagcgttct gaaggctata ttataggtga tgttgctggt
     1381 aaaaaagcta ttttggtgga cgatattctc aatactggtc gcactttctc acaagcttca
     1441 aaaattgtcc aagaaggtgg tgctactgaa atttatgcag ttgccagcca cggtctcttt
     1501 gctggaactg caacccaact cttagatgaa gcaccaatca aagaaatatt agttaccgat
     1561 tctgttgcaa gcaaagaaca gcatcctaaa aatatcgtct tcctaacagc tagtgacttg
     1621 attgcagatg ccatccaccg catccaagaa catcagccac tcagcccact tttcaaattt
     1681 actagtcctg aaaaagaaaa ctaggagtaa ctctttgatt tatttagata atgcagcaac
     1741 cactgctcta tctcctactg cactccaacg gatgatggaa gtggctcaag aaaattatgg
     1801 aaacccctcc agtattcatc aaagcggtcg gaaagccaat caacgtctta gacaaagtcg
     1861 tcaagagatt gctcagattc tctccgcctc tcccgaacaa attattttca catctggagg
     1921 ctccgaagct gatactttag ctattcaagg ctatgcactt gcgcatcaat ccaaaggaaa
     1981 acacctgatt actacggcta tcgaacacca tgctgtcctc cataccatgc agtatctaga
     2041 ggaacggttt gggttcgagg taacctatat ccaaccagtc gaccaagtaa tcaccgctca
     2101 acaaattcag gatgcccttc ggccagatac tattttagtc agcgtcatgt ttgctaacaa
     2161 tgagaccggt cagctacttc ccatcaagga aattggagaa ctcctagccg atcatcaggc
     2221 cgtctttcat gtagatgctg tccaagcaat tggaaaaatc gcaatatctc catctaacta
     2281 cggaattgac ttgctcgcgg cctctgccca taaattccat ggtccaaagg gaacgggatt
     2341 cctgtatagt aaagttcata aatttgattc cttgatccac ggcggaaagc aggaacagca
     2401 acatcgagca ggtactgaga atctccctgc tattgctgct atggcgactg ccctaacaga
     2461 acaatatgag tccttagata cgcatcatca acatgtcagt gaactgcgac aacatattat
     2521 cgagggattg tctggcttag actattatct caatcaggct ggacctcatc tccctcatgt
     2581 ccttaatatc ggttttcctg gtaaacttaa tgaacaactc cttatgcaac tagatttagc
     2641 aggtatcgct gtttctagtg gttctgcatg tacggcaggt gttgtccaaa atagccatgt
     2701 tttagaagct atgtatggaa aagactccca tcgtctgaaa gaatccattc gtatcagcct
     2761 atctgaaatc aatacaaaag aagagataga tactcttatc atcgaactaa agaaaatact
     2821 tggaggttaa aatggcattc caacaatcag ttaccttaaa agattgtaat tttacttatc
     2881 acattagccc tgctatcaaa aaatatacac tccgtgataa cacctttgaa caaaccaaag
     2941 caggtcacta tcagcttaca cgtcttctgg aagagattcc aaattcaaac caaggattcc
     3001 tcttgaaaat tattatcaat caagacctaa ctggttttaa aattaacatt accgacaagt
     3061 ctggtcttca tctggtaaac atttttaagc cagaaagtaa caaggttatt caagaaaaat
     3121 tctatttcct catggatagc ttgattgacc gtgacatctt tacaaaagag agttagtatg
     3181 attgaaattc actgtcttga cgcttacaag caagaacgta tccaaacatt tgagaataag
     3241 gatgcaacca ttctggcttt ttccggttgc ctaaccctcc cagactatta tccagttacc
     3301 agcattacac aagacggaca agctctggat tacaaaggga caatcggaga tctctatcgc
     3361 tatttacaaa cactagatta gacagtttcc tgtttaatct ttttttaaaa tcaatctgtt
     3421 gattttttca caaacttgga ctagaataga ataaagaact gtttaatata taaataatcg
     3481 ataaagcgta ttctatcgaa ataaaggagt ctaaagtgaa aaacgataaa aaatctgata
     3541 ttccacgcgc tactgccaaa cgtctatccc tctactatcg catttttaaa cgtttctatg
     3601 ctggaaatat tgaaaaagct agttcaaaag aaattgctga agctatcgga atcgattcgg
     3661 ctaccgtccg ccgagatttt tcctattttg gagagcttgg gcgtcgtggc tttggctaca
     3721 atgtcaaaga gttgatggat ttctttgcag atattctaaa tgatacctcc atcaccaatg
     3781 ttatgcttgt tggtgttggg aatatggggc gggccttgct acactatcgc ttccacgaac
     3841 gaaataaaat gaaaatagtc atggcttttg aagcagatga taatccagct gttggaacga
     3901 cagatgaaaa tatcccaatc catgcaatct cagagatcaa ggaacgtatc agcgaagcaa
     3961 actctcagac agccattcta acggtaccca gcgtcaaggc acaagaagtg accgatattc
     4021 ttgttgaagc tggtgtcaag ggaattctga gcttttcacc agttaacttg tctgtcccaa
     4081 aagatgtcgt tgtccagtat gttgatttga ctagtgaatt acagacacta ctctacttta
     4141 tgcgaaaagg ataagcaatg aaaaaaccag ttattggtat ttctggaaat gaatataaaa
     4201 caggggatca tacagaaccc ctgctttctt atacccaaac ttgcctcgta caagctattg
     4261 aagatgctgg cgggctccca ctcatccttc ctgttacaga gccagatttg gcaaaatatt
     4321 atatccatct tattgacaaa ctcatcctaa caggaggaca aaatgtccag ccaagttact
     4381 atcgcgaaga aagaactatt gacagcgata attatctacc aaagcgtgac gagtttgaat
     4441 tggccatcat aagggctgcc caagaaaatc aaaaacctat cttcggtatc tgccgaggtc
     4501 tacagctcta caatgtagct caaggtggta gccttcatca atcaatttct gaacattggc
     4561 aagacataga tggacaagaa gttagtcaaa cgattcaact gactcaaaat agtccacttt
     4621 acgacatata tgagtccgat ccgagtgtga actcatttca ccgtcaggct atcaaagact
     4681 tagcccctga cttagaaatc attgcactat cagataatca acagattatt gaagcagttc
     4741 attcagccta cccaactaaa tttttagggg ttcaatggca tcctgaacta ctctacggta
     4801 aacgtgagat tgaaaaagaa ctcttccact atattgtcaa taaactttaa aataactcac
     4861 tttcttctct aaaagaatag taatccttat ttccaacaat taaatgatct aataatacca
     4921 gtcctagctt ttcgcaggac tcttttaaat tctccgtaaa tagcaaatca ttcctactag
     4981 gttgtactga acccgacggg tggttgtgaa cgatgataat cgatgttgcc atacatttta
     5041 ctgcgtaatg caaaatttca cgtggttctg caatgctacg attaacactc ccaataaaaa
     5101 tcgttttttg actgataatc tggttctgtg tattgagata aagtgcaact aggtgctcct
     5161 gcttcttatg cccgatttca tgaatcatct tacgtcccaa cttctcactt cccaaaattc
     5221 tctcatttaa gagcagctct gattgattga tacgttttcc aagttcaatc atagccttaa
     5281 tttcaatcgc cttgacgcgc ccaattccag tcaaactttg caattcttca attgatagtt
     5341 ctcttagagc cgctaaactc tccaaacgat tcaataattt attggagaga atagaaacag
     5401 gttctttttt tgtcccagtt ctaataaaaa ttgctagtag ttcctgatta ctgagacgct
     5461 cagctccaac ttctaccaaa cgttctctag gtagaatggc ctcttctttg aattcaattt
     5521 gatacatcat ttcctcctca cctatctatt cgtaaaatct tgcaaaaaaa gaagccatat
     5581 aaaaccaaag attatctcct cagtcctata ccacttcttt atccttaata tatagatgtt
     5641 ttattgtcca taatcatact gattataact cttattgaag gaatctaaga tgtcttttgc
     5701 cgtttcatta tatggagtgg ttgattcaag aactcctttt acaacaatac gttgcgtagc
     5761 ataatagtcc gtacatgtca ctaacgtaac ttctgtacgt ccttctacat catcaatgac
     5821 atagacactt tcaggcgaaa caatttccac actatcaata acgtaggtgt aaacattggt
     5881 cttgtcagta atatagattt tcatgccgtt tttagcacga tcaagcggtg aaaagagaac
     5941 atctgccgca ccagttacac caaaaatatg gtggctagcc aacgcataat ttcctttccc
     6001 catctcttgg ttttccttca tggtaccagc tccatacatg agcgaagtat tgaagacccc
     6061 tttaaaaata ggaaggttga taccaagctc gggaaccgca atcccaccaa ttacaggtaa
     6121 gcgttgtgca tcccactgag ctgctaaaat agcttctgta gaaatagact gaacctgttc
     6181 gaaatcaaat gttgtctcag cctgtttatt cttttcgata tcctctgtcg taacattgct
     6241 aatctggtat ttattcgtat tccagccgat aataaaatta cggatagagg tattaaatat
     6301 caatgccaag gaaatcagta tcaatacaac tgttaaaaaa ttgcgccaaa aagaaccttt
     6361 acgcttcttc tttttttcac gttttgacat gattatttat tccttttctt ctttaatttc
     6421 ggcagcatct accaaggcaa aggtcatgat tttcgcttca tcattcaagc gcatcacttt
     6481 tacacccatg gttgaacgac cagtttgaga gatattggca acacttgtac gaataatcac
     6541 accagtatcg gtaataacca taatatcttc atcaccagat accgttgtca aacctgccag
     6601 agaaccattc ttatcagcaa ctttaagagt cttgataccc ttaccaccac gacctttggt
     6661 tggatactca ctggctggtg tacgtttacc aaaacctttt tccgtcaata cgagtacttc
     6721 ttggtcatca gaaatcatag tagcgccaac taactggtct ccctcgcgta aattgatacc
     6781 acgaacacct gttgcagtac ggcccatatt acgaacatct gtttcagtga aacgtacaga
     6841 ataaccgaac ttagtcccca taataatgtc tgcttgacca tttgttaaga aaacattgat
     6901 gagttcatcg tcttctttca aattcaaagc tttcagacca ctctgacgaa tattggcaaa
     6961 ttctgacaca ctagtccgtt ttacaacacc ttggcgggtt gcaaagaaga gatagctatc
     7021 tgcctcctgg tccttagtta cattgataat ggtttgaatt gcctcgtttt cttccaattt
     7081 aagcaaattg accactggaa gtccctttgc agtacgacca tattctggga tttcatagcc
     7141 tttgagacgg taaacacgtc ccttattagt gaagaagagc aagcgatcat gagtgcttgt
     7201 agagactaat tctcgtacaa aatcatcatc cttgacgcca gttccttgaa ccccacgtcc
     7261 tccacgcttc tgagcttgaa actcatcctg tgccaaacgc ttgatgtagc cttggtttga
     7321 caaggtgatt aaaacatccg tttcttcaat caagtcctca tcttcaaggg atagaacttc
     7381 accaaccatc aattctgtac ggcgtgggtc agcatattta cgtttgactt cttctaattc
     7441 gtctttaata atctgaacaa cacgctcagg cttagctaga atgtcagcca aatctgcaat
     7501 caacgccact aactcatcat attctgattg aatcttgtcc cgttccaaac ctgtcaaacg
     7561 acgaagacgc atatcaagaa tagcctgact ctgacgtaca gaaaggtcaa atttctccat
     7621 caattcagct tgagcaatag catcagtttc tgaattacgg ataatacgaa tgacttcatc
     7681 aatatggtca agagcaatca gcaagccttc taagatatga gcacgcgcct cagccttttc
     7741 tttatcaaat cgtgtacgac gagtgactac ttctttttgg tgttcaatat aagactctag
     7801 aatctgacgt acagaaagga ttttcggtac accattttgg atggccaaca tgttgaaact
     7861 aaagttggtt tgaagctgtg tttgcttgaa taggttattc aaaataacgt tagcagaagc
     7921 atcacgacga acttcgataa caaatcgtac accctcacgg ttggattcgt ctcgaacagc
     7981 agtgattcct tcaatacgtt tttcttggac taatttaaca atatgttctt gaaccttgga
     8041 cttattgacc atgtatggaa actctgtaac aacaatgcgt tcacgaccat ttccatattc
     8101 ttcaatttct gtgcgggaac gaagaactat tgaaccttta ccagtttcat aagcacggtg
     8161 aattccagat ttccccataa ccaaggcacc tgtcggaaaa tctggtccag gcaagacttc
     8221 cataatctca cgtgttgtta cctcgggatt gtccataacc agcttaactg cctcgatagt
     8281 ctcacccaag ttgtgtggag gaatgttggt agccatacca acggcgatac cagtcgttcc
     8341 gttaaccaga aggttaggga aacgagcagg taaaacctca ggctcacgtt cactcgcatc
     8401 atagttatct gcaaaattta ccgtgttctt gttaatatca cgaagcattt caagcgcaat
     8461 cttgctcatg cgtgcttctg tataacgctg agcggctgct ccgtcgccat ccatggaacc
     8521 aaagtttccg tgaccatcaa ctaacatgtg acggtagctc caccattgtg ccatacgaac
     8581 cattgcttcg taaatcgcac tatctccatg cgggtgatat ttacccataa catcccctgt
     8641 gatacgggct gattttttat gtggtttatc cggtgtaatt cctaattcat tcatcccata
     8701 caaaatacgg cgatgaaccg gcttcagacc atcacgtaca tcaggcagag cacgtgaaac
     8761 gataacactc atcgcatagt cgatgaagga tgatttcatc tcattggtca gattaaccgt
     8821 tactaaattt ttatcttgca ttatatgcct ctttcaacag tcattgtgct tctattatac
     8881 catattggcg aataaatttc tttgttggaa actagcatgt aaacattttc aaaggcgctt
     8941 tcacaagtga caaaaagctt aaaacatgct agaatgtatt atggtcggga tgaagtcatc
     9001 cccaaataaa actaaggaga tgtttagcaa tgactgcaac taaacaacac aaaaaagtaa
     9061 tccttgtcgg tgacggtgcc gtaggttctg cttatgctta tgctcttgtt aaccaaggga
     9121 ttggtcaaga attgggtatc atcgatatca acaaagaccg tactcaaggt gatgcagaag
     9181 acttgagcca cgcattggcc ttcacattcc ctaaaaaaat ctactctgct gagtattcag
     9241 atgctcatga cgcagacttg gttgtattga cagctggttt gccacaaaaa ccaggtgaaa
     9301 ctcgccttga attggtagag aaaaaccttc gtatcaacca acaaatcgtt acagaaatcg
     9361 ttaactctgg tttcaacggt atcttccttg ttgctgctaa ccctgttgac gtattgactt
     9421 actcaacttg gaaattctct ggtttcccta aagagcgtgt tatcggttct ggtacttctc
     9481 ttgactcagc tcgtttccgt caagcattgg ctgagaaaat cggtatcgac gcacgttcag
     9541 ttcacgctta catcatgggt gagcacggtg actcagaatt tgcagtttgg tcacatgcca
     9601 acgttgccgg tgtgaaattg tacgactggt tgcaagataa ccgcgatatt gatgagcaag
     9661 gtcttgttga cttgtttgta tctgttcgtg atgcagccta ctcaatcatc aataaaaaag
     9721 gcgcaactta ctacggtatc ggtgttgcac ttgcacgtat cactaaagca atctttgatg
     9781 atgaaaatgc agtacttcca ttgtcagttt accaagctgg ccaatacgaa ggtgttgaag
     9841 atgtcttcat cggtcaacct gctatcatcg gtgcccacgg tatcgttcgt ccagtcaata
     9901 tcccattgag cgacgctgaa ttgcaaaaaa tgcaagcatc tgctaaacaa ttaaaagaca
     9961 tcatcgatga tgctttcgca aacccagaaa ttgcagctgg tgttaaaaac taattatata
    10021 aaatagtaaa aaaggtgagc aatagctcgc cttttttacc ttctaatatc ataacgtaac
    10081 atagcaattc catctgtgac agtggactct accaagagca atttcttttc ttccaaaagt
    10141 gaggcgaaaa gtggaatacc gtcacccaaa atagtaggaa taatcccaat aataaggtga
    10201 tcaatcactc ccgcctctaa acagtcctga accagacttg ctccaccaaa caaccaaata
    10261 tctccacctt ctctagaacg aagttccata atctccccga tgatgtccct tacgaaacgc
    10321 acattaccat agtccgtctg ctcggtacga ctcgcaacga taaattgctt ttcctgatat
    10381 ccttcaatca tttccaacgg acaatcttct aatgattttc tccccatcgg gatcgggaaa
    10441 gaactcgact ggtcaaaaac atactaagat tagtagtgag gaaat
//
LOCUS       DASFSW010000073        10430 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.073, whole genome shotgun sequence.
ACCESSION   DASFSW010000073 DASFSW010000000
VERSION     DASFSW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.073"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(3..911)
                     /locus_tag="U1099_001336"
     CDS             complement(3..911)
                     /locus_tag="U1099_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEM4867655.1"
                     /translation="MAEKRFGILITLIAGIAWGLSGVSGQYLMSRGVSVDMLTSLRLL
                     VSGFFLVGLAYATAKEQLLIVLKNRKALLGIFVFAMLGLVLNQMAYLQAIYHTNAGTA
                     TVLQYLCPILVLAYTCLKNREKPSGIELISIILAVAGTFLIATHGKLDELAVTPIGLF
                     WGIFSAFTYALYIILPGKLIRQYGSITVIGLGMLMGGFVVTLGLQTWQHRLPIDGGSF
                     LGLLGIVGVGTIFAYTAFLKGVSMVGPVNGSLLASIEPIASVFFAVWLVNEQFYAIDF
                     VGMLFILLAVLLISLKDILVAQKSIG"
     gene            complement(986..2467)
                     /gene="galT"
                     /locus_tag="U1099_001337"
     CDS             complement(986..2467)
                     /gene="galT"
                     /locus_tag="U1099_001337"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEM4867656.1"
                     /translation="MMAGLVDRFVEQVIANSDFEEMDALYLHNRVLALVGDQVLTVQT
                     DLEDLIELKDELLAHGVRTGFVGELLEEQDMVGACLMDLMTPSPSQVNRDFWQTYQDS
                     PEQAIGDFYELSKRNDYIKMAAIAKNIYYQVSTEYGDLEITINLSKPEKDPKSIAAAT
                     KAEASNYPKCLLCMENEGYQGRINHPARANHRIIRLELGQEKWGFQYSPYAYYNEHAI
                     FLNQEHVPMVISPRTFEQLLDLLDLLPGYFVGSNSDLPISGGSILTHNHYQGGRHSFA
                     MEKAPIERQLVFDGFESVSAGIVKWPMSVIRLSSADKPSLLGLATKILEKWRSYSDDN
                     VQIKAETDGTPHHTITPIARKRGDLYELDLVLRDNQTSEEFPDGIYHPHPDVQHIKKE
                     NIGLIEVMGLAILPPRLKAELAEVEKFLLGQDSQVADYHQPWAESLKTAHPDVTEETV
                     EQVVRESVGQIFARVLEDAGVYKRTPEGQAAFLRFVEFVGLAT"
     gene            complement(2477..3649)
                     /locus_tag="U1099_001338"
     CDS             complement(2477..3649)
                     /locus_tag="U1099_001338"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEM4867657.1"
                     /translation="MNTEQLKQAFLDVFGQEADATFFSPGRINLIGEHTDYNGGHVFP
                     AAITLGTYGAARKRDDQLLRFYSANFEELGIIEVDLNNLVFDKADNWTNYAKGVLKFL
                     QEAGHAIDTGMEVFVYGNIPNGSGLSSSASLELLIGIIAEELYGLELTRLDLVKIGKQ
                     TENHFIGVNSGIMDQFAIGMGADNRAIYLDTNTLEYDLVPLDLGDHVIVIMNTNKRRE
                     LADSKYNERRAECEKAVEELNAVLNIQTLGELDEWTFDQYSYLIKDENRIKRARHAVL
                     ENQRTLQARKALEEGDLATFGRLVNASHVSLEHDYEVTGLELDTLAHTAWEQEGVLGA
                     RMTGAGFGGCGIAIVHKDKVEAFTENVGKTYTEVVGYEPSFYVAEIAGGSRVLSRK"
     gene            3782..4780
                     /locus_tag="U1099_001339"
     CDS             3782..4780
                     /locus_tag="U1099_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM4867658.1"
                     /translation="MVTIKDIAKQAHLSSATVSRVLKGDLTLSVSQETRDRIFNLAQD
                     LGYTKHLKKQAPQQKGTIGIVQWYTESEELADLYYYSIRASIEQTASLLGYQIIRSFN
                     DLTHPLLQEVDGIIAVGKFSSGQIAELTNLSSKLIFVDSDTLTEGFSCITTDFEHSVQ
                     TVIDHFRSQGLTDIGLLVGQEETTDGYQLPTDPRLTAFRDYLDTLGMLQEDYIYQGKF
                     STQSGYELMSQAIEDLGDQLPPAFFMANDTLAVGALRALQERQIAVPGRVQLITFNDT
                     AITRQVYPALSSISVFTEEMGQEAMQLLDRVIASPQPHHPRKIKLGTQLVIRESSY"
     gene            complement(5083..5238)
                     /locus_tag="U1099_001340"
     CDS             complement(5083..5238)
                     /locus_tag="U1099_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEM4867659.1"
                     /translation="MPMGNSSKSDGKEEHLLTAFEVVQTILGFGSFTIALIGLCYKIF
                     KDNDKKK"
     gene            complement(5556..6995)
                     /gene="gatB"
                     /locus_tag="U1099_001341"
     CDS             complement(5556..6995)
                     /gene="gatB"
                     /locus_tag="U1099_001341"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEM4867660.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKDMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FNYVRKGLQHEVERQAKILRSGGQIQQETRRYDESTGETILMRVKEGSADYRYFPEPD
                     LPLYEIDDSWIDEVRAELPVFPKARRAHYVENLGLTTYDAGQLTSTKALSDFFEAAVA
                     AGGDAKQVSNWLQGEVAQFLNAEGKTIEQIALTPENLVEMIALIADGTISSKIAKKVF
                     VHLAKEGGSAKAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELAKLLD"
     gene            complement(6995..8461)
                     /gene="gatA"
                     /locus_tag="U1099_001342"
     CDS             complement(6995..8461)
                     /gene="gatA"
                     /locus_tag="U1099_001342"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEM4867661.1"
                     /translation="MTFNNKTIDELHDLLVKKEISALELTKATLEDIKSREGAVDAFL
                     TITEDAALAQAAALDEKGIDADNVMAGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVSQAYAKDMIVVGKTNMDEFAMGGSNENSAFKPTKNAWDQTKVPGGSSGGSAAAV
                     AAGQVRLSLGSDTGGSIRQPAAFNGIVGMKPTYGTVSRFGLIAFGSSLDQIGPFSQTV
                     KENAQLLNVISGHDVKDATSTINEIADFTSKIGQDIKGMKIALPKEYMGEGIDPQVKE
                     TILKAAKHLESLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRAEDA
                     TNLEEIYVKTRSQGFGEEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGLSIPAGFVEGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGAN"
     gene            complement(8461..8763)
                     /gene="gatC"
                     /locus_tag="U1099_001343"
     CDS             complement(8461..8763)
                     /gene="gatC"
                     /locus_tag="U1099_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEM4867662.1"
                     /translation="MKISEAEVRHVAKLSKLEFSDQETAEFATSLSKIVDMVELLNEV
                     DTTGVAVTTTMADRKNVLRADIAQKGESREELFKNVPETQDNFIKVPAILDGGGDA"
     gene            complement(8981..9655)
                     /locus_tag="U1099_001344"
     CDS             complement(8981..9655)
                     /locus_tag="U1099_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEM4867663.1"
                     /translation="MSEQFIPSVLSDLRQDIVQMPEVIKECSGIRIYGRRIRSVLFTT
                     DVSIIANHNADAILAVYPFTPSPAIIKSIMLVASVPVLAGVGGGLTTGMRSANMSLLS
                     ESEGAYAVVVNGPTDVKTIEAINKVVDIPIIYTVVSEKSDLTSRIKAGVDILNVSCGM
                     ETPRVVKKIREAFPDFPIIATGGPTDDSIRRVIEAGANAVSYTAPSNGELFKGKMEKY
                     RKHAKD"
     gene            complement(9839..10186)
                     /gene="rplS"
                     /locus_tag="U1099_001345"
     CDS             complement(9839..10186)
                     /gene="rplS"
                     /locus_tag="U1099_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEM4867664.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGVGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
BASE COUNT         3059 a         2604 c         1945 g         2822 t
ORIGIN      
        1 tattaaccaa tactcttttg ggcaaccaat atatctttta aagaaataag caaaacagca
       61 agtaaaataa acaacattcc aacaaaatca attgcataaa attgttcatt gaccaaccat
      121 actgcaaaaa agacagatgc aatcggctca atcgaggcca aaagactgcc attgacagga
      181 ccaaccatac tgacgccttt aagaaaggca gtataggcaa aaatcgtccc gactccgaca
      241 atacctaaca ggcccaggaa acttccacca tcaataggaa gacgatgctg ccaagtttgc
      301 aaaccaagcg tgactacaaa accacccatt aacatcccta aaccaatcac tgttatactg
      361 ccgtattgac ggatcaattt acctggcaaa ataatatata gcgcataagt aaaggctgaa
      421 aagattcccc agaacaaacc gattggagtc actgccaatt catctaattt tccatgagtc
      481 gcaatcaaaa aagttcctgc tacggctaag ataattgaaa tcaactcaat ccctgatggt
      541 ttttccctat ttttcaaaca ggtataagcc aagactaaga tagggcaaag gtattgtaaa
      601 accgtagccg ttccagcatt ggtatgatag atggcttgta aataagccat ctggttaaga
      661 accagaccca acatcgcaaa aacaaaaatt cccaacaagg cttttctatt tttcaacact
      721 atcaacagct gttctttcgc tgtagcataa gccaggccaa cgagaaagaa tcccgatacc
      781 aacaaacgca gggaggtaag catatccact gaaacaccac gtgacatcaa gtactgtcca
      841 ctcactccag acaagcccca ggctatacct gctattaatg taatcagtat cccaaatctc
      901 ttttccgcca ttatatctcc ttctcacact gtgaatactt tcattatacc aaaagagcag
      961 gacaccgtcc cactctttct tgcaattatg ttgctaaacc tacaaattct acgaatcgta
     1021 gaaaggctgc ctgtccttct ggtgttcgtt tgtagacccc tgcatcttcc aatacacgcg
     1081 caaaaatttg tcccactgac tcacgaacaa cttgttcgac agtttcctct gtcacatctg
     1141 ggtgagctgt tttcaggctt tccgcccaag gctgatgata gtcagccacc tgactatctt
     1201 gacccagtaa gaatttttct acttcagcca attcggcctt caagcgtgga ggtaaaatcg
     1261 ccaagcccat tacctcaatc aagccgatgt tttctttctt tatatgttgc acatctggat
     1321 gtggatggta gatgccatct ggaaactctt ctgaggtctg attgtcgcgg agaaccaaat
     1381 ccagctcgta caaatctcct cgtttccgag caatcggggt gatggtatga tgaggagttc
     1441 catccgtctc agcctttatc tgaacgttat catctgagta gctccgccat ttttccaaaa
     1501 tcttagtcgc taaaccaaga agcgatggct tatctgccga actcaagcga atcaccgaca
     1561 taggccattt gacaattcct gccgaaacgg actcaaagcc gtcgaagacc agctggcgct
     1621 caatcggcgc cttttccatg gcaaaactgt gccgcccacc ctgatagtga ttgtgggtca
     1681 agatggagcc acccgagata ggtaaatcag agttggaacc aacaaaatag cctggcaaca
     1741 aatccaacaa gtccaagagc tgctcaaatg ttcgcggact aatgaccatc ggcacatgtt
     1801 cctgatttag gaaaatagca tgttcattgt agtaagcata gggcgaatac tggaagcccc
     1861 acttttcctg tccaagctcc aagcgaataa tgcggtggtt ggcgcgtgct ggatggttaa
     1921 tccgaccctg atagccctca ttttccatac agagtaggca tttgggataa ttgcttgctt
     1981 cagctttcgt agcagctgca atagacttgg gatccttctc aggtttagac agattgattg
     2041 tgatttccaa atccccatac tctgtcgaaa cttgataata gatatttttc gcaatcgcag
     2101 ccatcttgat atagtcattg cgcttgctca attcatagaa gtcaccaatc gcttgctctg
     2161 gactgtcctg ataggtctgc caaaaatcac gattgacctg acttggactt ggtgtcatca
     2221 aatccatcaa acaagcccca accatgtcct gctcttccag caattcaccc acaaaaccag
     2281 ttcgaactcc atgagccaat agttcatctt ttaattcaat caagtcctct aaatctgttt
     2341 gaacagtcag aacctggtct cccaccaaag ccaagacccg attgtgcagg taaagagcat
     2401 ccatttcctc aaaatcactg ttggcaataa cctgctcaac aaaacgatcc accaatccag
     2461 ccatcatttc tccaatctat tttcgagata aaacgcgaga gcctccagca atctccgcta
     2521 catagaagct tggttcatag ccaaccactt cagtataggt cttgccgaca ttttccgtga
     2581 aggcttcaac cttatccttg tggacaatgg caataccaca gccgccgaag ccagctcctg
     2641 tcatccgagc accaagaact ccttcttgct cccaagctgt gtgagccaag gtatccaatt
     2701 ctagacctgt cacttcataa tcatgctcca aagaaacatg ggaagcattg accagacgac
     2761 caaaggtagc caaatctcct tcttctaaag ccttacgagc ttgcaaggtc cgttgatttt
     2821 ctaagacagc atggcgagca cgcttgatac ggttctcatc tttaatcaaa taactatatt
     2881 ggtcaaaggt ccactcatcc aattcaccta gagtttgaat attcaagact gcattcaatt
     2941 cttccacagc tttttcacat tctgcacgac gctcattgta cttagaatct gccaattcac
     3001 ggcgtttatt ggtgttcata atgacaatca catggtcccc taaatccagc ggtaccaagt
     3061 catattccaa ggtattggta tcaaggtaaa tcgcccgatt atctgctccc ataccgatcg
     3121 caaactggtc catgatccca gaattgacac cgataaagtg attttccgtt tgtttcccaa
     3181 ttttcaccaa atcaagtcga gtcaattcaa gtccatacaa ctcttctgcg ataatgccaa
     3241 tcaagagttc caaggaagct gatgatgaca agcctgaacc atttggaatg ttaccgtaaa
     3301 caaagacttc catccctgta tcaatggcat gccctgcttc ttgcaagaat ttgagaacac
     3361 ccttggcata gttggtccag ttatctgcct tatcaaagac cagattgttc aaatccactt
     3421 cgataattcc aagttcttca aagttagcag aataaaaacg caaaagctgg tcgtcacgtt
     3481 tgcgagcagc cccgtaggtc cccaaggtaa tagccgcagg gaaaacatga ccaccattgt
     3541 agtccgtgtg ctcaccaatc aaattaatcc gacctggtga gaagaaagtc gcatctgcct
     3601 cttgaccaaa cacatcgaga aaggcctgct tgagttgttc tgtgttcata agaacctcct
     3661 agattttgta atcgttttct tttattgtac aactcaacaa tttaaaagtc aatattttta
     3721 ctaaattttt actaattttg ttttttatgc tatactagag acaaataaac aggagaagaa
     3781 catggttaca atcaaagaca tagcaaaaca agcccacctt tcctctgcca ccgtttcccg
     3841 cgttctcaag ggagacctga ccctatccgt cagccaagaa acccgcgacc gcattttcaa
     3901 cttggcacag gacctgggct acaccaaaca cctcaaaaaa caagctcccc aacaaaaagg
     3961 caccatcggt attgttcagt ggtacaccga gagcgaagag ctggctgacc tctactacta
     4021 ttctattcga gctagcatcg agcaaaccgc cagcctcctg ggctaccaaa tcatccgctc
     4081 ctttaacgac ctgacccatc ccctcctcca agaggttgac ggcatcatcg cggttggtaa
     4141 gttttcttct gggcaaatag cagagctaac caacttgtct tccaagctca tttttgtgga
     4201 ctctgataca ctgacggagg gattctcctg tatcacgacc gactttgaac actctgtaca
     4261 gaccgttatc gaccacttcc gttctcaagg tctgacagac attggcctcc tagttggaca
     4321 ggaagaaacc acagacggct accaactgcc aacagatccc cgcctgactg cctttcgtga
     4381 ctacctagac accttgggca tgctccaaga agactacatc taccaaggaa aattttccac
     4441 ccaatcagga tatgagctga tgagccaggc tattgaggac ctaggcgacc agctaccccc
     4501 tgcctttttc atggcaaatg ataccctggc agtcggagcc ctgcgtgctt tacaagagcg
     4561 tcaaatcgca gtgcccggta gagtccaact catcaccttt aacgacacag ccatcacccg
     4621 ccaggtctat ccagccctgt cttccatcag cgtcttcacc gaggaaatgg gccaggaagc
     4681 tatgcaactg ctggaccgtg tgattgcgag tccgcagccc caccatcccc gtaagattaa
     4741 gttgggtacc cagctagtaa tacgcgagag ttcttattga taaggaaatc ttccatcatt
     4801 ccgttactta tattgaattt tatacaagtg gtttgatacc gagtacggct tttcaataag
     4861 tattaaagat gttaaacttc ttgtaaaact tgtactaaaa tttgcatagc acatgctcat
     4921 agccaacatg aacagattat ttgcatttta caagtaatct ggtataatgg atatagaaaa
     4981 aggagctgga cggcaatcca gccccaaatg tagaaccgtt aaaaagacgg tggctcagtt
     5041 tagattattt agaaataacc gttactgtcc aaagtttgac ggttatttct ttttgtcatt
     5101 atctttgaag attttatagc acaagccaat caatgcaata gtaaaactac caaagcctag
     5161 aatagtttgc acaacttcaa aagctgtcaa aagatgctcc tcctttccgt cagattttga
     5221 tgaattgccc ataggcatca cctcactttc agaaaatcaa gagccaccgt cttcactttt
     5281 ctacccatct agtataacat actcattcct aaaaattatt ggaatagtgc agtattcccg
     5341 aaatctttga gaacccccaa aaaattacaa ctcttctata gtgatgatat tttttcattt
     5401 tagctaacta tcctttaaca gtcaataccc gcgctacctt ctaatacttt ataccgtttt
     5461 taaaatatag cataaaaata agatagaagc acctagttta caagtaacag taaaaaccgc
     5521 taagattcaa tcctagcggt tttcttctat tcaatttaat ccaacaactt agccaattcc
     5581 tgagccaaga gttgttgtgc aacttgcggg ttagcttgtc ccttagtcgc tttcatcaag
     5641 aaaccagtaa aggccttgtc tgcattgcgt ttacctgact tgaagtcagc tacagccgct
     5701 tcgttatccg caaagacttg gtggataatc ggaatcaaga ccgcagggtc tgaaatctgt
     5761 accaaaccag ccttctcaac ataagcctta gcagagccgc cttccttagc caagtgaaca
     5821 aagactttct tagcaatttt ggatgaaata gtgccatccg caatcaaggc aatcatctca
     5881 accaagtttt ctggtgtcaa agcgatttgc tcaatagtct taccttcagc attgaggaac
     5941 tgagccacct caccctgcaa ccagttggat acctgcttag catcgccacc tgctgccact
     6001 gctgcttcaa agaagtcaga cagagccttg gttgatgtca actgaccagc atcgtaggta
     6061 gtcaagccca agttttccac atagtgagca cggcgagcct ttggaaagac tggcaattct
     6121 gcacgcactt catcaatcca gctatcatca atctcgtaga gcggtaggtc tggctctggg
     6181 aagtaacggt agtccgctga accttctttg acacgcatga gaatggtttc accagtagat
     6241 tcatcgtaac gacgcgtttc ctgctggatt tgaccacctg aacgcaagat tttggcctga
     6301 cgttctactt cgtgttgcaa gcccttgcga acatagttga aggagttgag gtttttcaac
     6361 tcagtcttgg taccaaattt ttcttgacca tagggacgaa gagagatgtt ggcatccacg
     6421 cgcatagaac cctcttccat cttcacgtct gaaataccag tgtattggat aatttccttg
     6481 agagcagtca agtaggcata ggcctcctca ggcgaacgca tatcggcttc tgatacaatc
     6541 tcaatcagtg gcactccttg acggttgagg tccacataag aataaccgtc tgtcccgtgg
     6601 gtattcttac cagcatcctc ttccaagtgg gcacgctcga tacggatttt cttggtcgaa
     6661 ccatcttcta actcaatctc aatccagcca ttgtagccaa tcggctcgtc aaactgggaa
     6721 atctgatagg ctttagggtt atcaggatag aaatagttct tacggtcaaa gtgcatgtcc
     6781 ttatgaatgt ccatgttcaa agccaaggca gccttgattc ctgcgtccac aacgccctta
     6841 ttaagaacag gaaggacacc tgggaaagac cagtcaatca cgttggtatt ggcatttgga
     6901 tcctcaccaa aatgagcaga tgaaggtgag aaaattttcg agttggtatt caactccaca
     6961 tggacttcta gaccaataat cgtttcaaag ttcattagtt agcacctcca aaaatcactg
     7021 gttgttgctt gtggtagtct gttgtcgctt caaaggcagc agccacttgg taaatggtct
     7081 cttctgaata ttttggacca atcaactgca aaccgactgg caagccttca acaaaaccag
     7141 caggaataga aagaccagga agacctgcta agtttacagg aattgttaac aagtccgcca
     7201 agtacatagc cacagggtca tggttgagcg tgtccaaacc aaaggcaacc gtcggagctg
     7261 ttggccccaa aatcaagtca tagtctgcaa agactttttc aaaatcctgg ataatcaagg
     7321 tccgtacctg accagccttc ttgaagtagg catcgtagta accagatgac aagctaaatg
     7381 tacctaacat aatacgacgt ttgacttcct caccaaaacc ttggctacgg gttttcacat
     7441 agatttcttc caagttggtc gcatcttctg cacggaaacc ataacggata ccgtcaaaac
     7501 gttgcaagtt agaagacgcc tctgatgaag caatgatgta gtaaacagca accccatact
     7561 tagaatgtgg caggctgact tcttcaatga ttgctcccaa gctttccaag tgcttagccg
     7621 ccttgagaat agtttccttc acctgcggat caatcccttc tcccatgtat tcttttggca
     7681 gagcaatctt catacccttg atgtcctgac caatcttgct agtaaagtct gcaatttcat
     7741 tgatggttga tgttgcatcc ttgacatcat gaccagagat gacattgagc aactgggcat
     7801 tttccttaac cgtctgtgag aatggaccga tctggtcaag agatgaacca aaggcaatta
     7861 aaccaaaacg tgacaccgtt ccataggtcg gtttcatacc gacgatacca ttaaaggctg
     7921 ctggttgacg gatagagcca cctgtgtcag aaccgagtga taaacggact tgaccagccg
     7981 caactgcagc agcggagcca cctgaagaac caccaggaac ttttgtctgg tcccaagcat
     8041 ttttggtcgg tttgaaggct gagttctcat tagaaccacc catagcaaac tcatccatgt
     8101 tggtcttacc aacaacaatc atatccttgg cataggcttg ggaaaccgat gtcgcatcga
     8161 aaatcggctc gtagttatag agcatttttg aagctgccgt ggtcaaaatc cccttagtag
     8221 agatattgtc cttgactgcc aaaggaatcc ctgccataac attgtccgca tcaatccctt
     8281 tttcatcaag ggcagcagcc tgcgccaagg ccgcatcttc tgtgatagtc aagaaagcat
     8341 ccactgctcc ctcacggctc ttaatgtctt ccaaagttgc cttggtcaac tcaagagccg
     8401 aaatctcctt tttgacaagg aggtcatgca attcatcaat ggttttattg ttaaaggtca
     8461 ttaggcatct cctcccccgt ctagaatagc tggtaccttg ataaagttat cttgggtctc
     8521 aggcacattt ttaaagagct cctcacggct ctcacctttt tgggcaatat ctgctcgcaa
     8581 gacattctta cggtcagcca tggtcgttgt caccgcaaca cctgtcgtat ccacttcatt
     8641 gagcaactca accatatcga caatcttgct caaacttgtc gcaaattccg ctgtttcttg
     8701 atccgaaaat tccagcttag acagcttggc aacgtgacgg acttcagctt cagaaatctt
     8761 cattcttact ttcctcctaa tggaaatcta tcttctcatt ataccatatt tccagcccca
     8821 gacaaaggta gaaatgacct aatatgttac caatttcctc ctaaaaatcc attctttaaa
     8881 ttgcgaagta ttcctaatct cacaccgtaa aacgataaaa aacagcaagt ttcatctagc
     8941 aataaaactt actgtttgct tagcctatct tttttatgag ctaatccttt gcatgtttgc
     9001 ggtatttttc cattttccct ttaaaaagct ccccattact tggtgctgta taggagacag
     9061 cattggctcc agcttcgata acccgacgaa tagagtcatc agtcggacca ccagtcgcaa
     9121 taattggaaa gtcagggaaa gcttctcgaa tcttcttcac aacccttggc gtttccatac
     9181 cgcaagaaac atttaaaata tctactccag ctttaatccg tgaggttaaa tcagactttt
     9241 cagaaacaac ggtataaatg ataggaatat ctactacctt attgattgcc tcaattgttt
     9301 ttacatccgt tggtccattt accacaacag cgtatgctcc ctccgactca gacaatagac
     9361 tcatattagc tgaacgcata cctgtcgtca agcctccacc aacaccagcc aagactggaa
     9421 ctgacgcaac caacataata cttttgataa tagctggact gggcgtaaat ggataaacag
     9481 ccaaaatagc atctgcattg tgattggcaa taatagacac atccgttgta aataagactg
     9541 aacgaatgcg acgaccataa atacgaattc cactacattc cttgataact tctggcatct
     9601 gaacaatatc ctgccgtaaa tctgataaaa cagatggaat aaactgttcg ctcatccgca
     9661 acctcctcta tactagctac aataatatat agtataacat attatttcat ggaagataca
     9721 caaaaacagt ccattttacc actgctttca caattaaata ctttttcaac agattaaaaa
     9781 aaaaaagaca gctttcgctg cctcccattc tccgaccaaa atagtctgta gaactatctt
     9841 aacgacggat ttctttgata cgtgctgctt taccttgcaa tgcacgtagg tagtacaatt
     9901 tagcacgacg tactttaccg taacgtacta cttcaatctt atcaacacgt ggagtgtgga
     9961 ttgggaatgt acgttcaaca cctacaccac cagagatttt acgtacagtg tacatttctg
    10021 agatgccttg acctttacga gagataacaa caccctcgaa aatctggata cgttcgcggt
    10081 taccttcgac aaccttagcg tgaacacgca cagtgtcacc aggacggaat gaagggatgt
    10141 cagtacgaag ttgaccttct gtcaaacttt ggatcaatgg attcatttta ttctcctatc
    10201 tttgtcaatc ataggtgctt ttaaacccag cggataaact gtatttttgt gcgtccatta
    10261 cacacagact atattataca ccaagtaatc acctatgtaa agcattttga taaatttatc
    10321 atggtcaatc acaccgcttc ttttacaagt taagtcaaca aggtctgagt ttgattttcg
    10381 aagagtgtta ataatacaga ttcctattgg agaaaaatga aaagaggctg
//
LOCUS       DASFSW010000074        10365 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.074, whole genome shotgun sequence.
ACCESSION   DASFSW010000074 DASFSW010000000
VERSION     DASFSW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.074"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..2665
                     /gene="smc"
                     /locus_tag="U1099_001346"
     CDS             <1..2665
                     /gene="smc"
                     /locus_tag="U1099_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEM4867665.1"
                     /translation="LDLTKLISDLERKIEVHKLESSQNESSRQEAQARLENLLTRREQ
                     LAEQIEQKQETLAQLDSSLSSLKDDIAAVDKEISYFSEDPDQVLDHLREQYVALMQEE
                     AEASNSLTKIQQDIANQISLSESKSEDLARLQTEKQTAQEVLDKSRKSLEEADHVLRQ
                     LLESYQIKKSELDQTQTAYQAEQGRLFDLLDQLKGKQARQSSLEAILKNHSNFYAGVK
                     AVLQAAPSLGGIIGAVSEQLTFDTRYQTALEIALGGASQNVIVEDEAIAKRAIAFLKE
                     KRQGRATFLPLTTIKPRQLSGQQVSLLESSEGFLGLASDLVTYQPNLDAIFQNLLGTI
                     AIFDSIDHANQAARATKFQVRMVTMDGAEIRPGGAFAGGSNRNNSTTFIKPELDALLG
                     EIAELSSQLQEQESLVAAKKTSLDQTREALETIKAEGEEARLNQQSARIHQEQAENRL
                     AQLSAQYDLQMSQVSPTILTELEEAAAKEEVNVQALNEKKLALDQQINQVRDNRDSIQ
                     ESLQKLQTQKGQLTLEQAELTSQLRFEQTDLRRLQEEKVVADKEISLLEDMIDQKLEA
                     LEDTSIEILEEQLQAASDKQNQTNQILIRLKFELEDIDGQFEDLEERLQQARTKNDDL
                     IRKQAKLEADCEQAGDKLRTLLGNLTEHFKLSFEAAQSQAKEVENLAVAEQSLKDLER
                     AIRALGPVNLDAIEQYDEVNNRLTFLNEQRTDILSARDLLLDTIHEMDDEVKERFKVT
                     FEAIRESFKQTFKQMFGGGSADLILTEGNLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKESQFIVVTHRKGT
                     MAAADAMYGVTMQESGVSKIVSVKLKDVEKL"
     gene            2662..3471
                     /locus_tag="U1099_001347"
     CDS             2662..3471
                     /locus_tag="U1099_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4867666.1"
                     /translation="MINKIYASDMDGTFLREDHSFDKERFRRILNQFKEKGYLFVATS
                     GRSMQSLKLVFEDFVDEIGFVAENGSIVEYQGQTIFMDDPISPEIYLPIIAGIDAGPF
                     GSSRSMVLSGLENFYLLKNAEPQFLEAMTNYYAHFRLVDTFEEVREEIIKINAKFSPE
                     EMDDARRWLNDTFEGVTAMTTGFDNIDIIPNGSNKSVGLSHLCAHFGITRQDVVAFGD
                     NQNDLDMFDFAGLALATENAREEVKAQADWMIGHCNDGAVLAYLEEEVNGN"
     gene            3461..4273
                     /locus_tag="U1099_001348"
     CDS             3461..4273
                     /locus_tag="U1099_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM4867667.1"
                     /translation="MAIKLIALDLDGTLLTSDKRISEANKKALQAARERGVYVVLTTG
                     RPLQAIGTFLEELDLLGENQYSITFNGGLVQENTGRVLDKVGFTIEDVRTIRRVTNDL
                     DLPLDVLYGGDVYSLPSAHESLYLTCNPLLNKIVASDEEMPEDFVYNKAVSAVAADFL
                     DGQIPKIPAELHERFEIFKSRDILLEWSPKGVHKANGLAKLIGHLGIDQSEVMACGDE
                     GNDLSMIEWAGLGVAMANATDEIKSAANLVLPKNNDEDGIAWVIEHYVLNED"
     gene            4275..5549
                     /gene="ftsY"
                     /locus_tag="U1099_001349"
     CDS             4275..5549
                     /gene="ftsY"
                     /locus_tag="U1099_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEM4867668.1"
                     /translation="MGLFDRLFGQKKQEEQVALVEEVEREQSASSEDTVTTEVESATQ
                     EMEKFQSEVAETELTAEQEAQKQRTLDMMAQYYAAKEAAAARVKEAQEQGFDPAIQTK
                     VKEEPVEEVAPVGQESEQDKYQRTLKKTRTGFGARLNEFFANFRSVDEEFFEELEEML
                     ILSDVGVQVASTLTEELRYEAKLQNAKKTDELRRVIIEKLVDIYEKDGQFSERINFQD
                     DLTVMLFVGVNGVGKTTSIGKLAYKYKQAGKKVMLVAADTFRAGAVAQLVEWGRRVDV
                     PVVTGPEKSDPASVVFDGVKRAVAEGVDILMIDTAGRLQNKENLMAELEKIGRIIKRT
                     LPDAPHETLLALDASTGQNALSQAKEFAKITPLTGLVLTKLDGTAKGGVVLAIRQELD
                     IPVKLIGFGEKIDDIGEFKSEEFMRGLLEGLV"
     gene            complement(5573..6130)
                     /locus_tag="U1099_001350"
     CDS             complement(5573..6130)
                     /locus_tag="U1099_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867669.1"
                     /translation="MQHKGTQILETERLILRPFQASDVESVFQNWTSDEKVTTYLTWP
                     THQTLQDSEDYVQFCLQSYSQEKTYRWVIELKENQQPIGDISVVSLDERVQAAELGWV
                     LGSKWWGQSYMAEALEAVNHYLLEEVGCLRITAVHDSENRPSGRVMEKVGMTYEGTLR
                     QAARNNRGIVDIAIYSLLHTDRKSR"
     gene            complement(6132..6806)
                     /locus_tag="U1099_001351"
     CDS             complement(6132..6806)
                     /locus_tag="U1099_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEM4867670.1"
                     /translation="MTELNTILTQLQAASHAGTLKRYEKIGETKPYYGVPMGAISGIA
                     KAYKNRLDLFAPLWQTGVLEAQYLAIQIAKNKPDQLPQADLENCLNEQISVNVLDKLA
                     SIILSKRKDSRVWEEDLLAKDQAIFQRLGWFLRAKYFAGKTAKNQEIEETLDHIRQHL
                     QTADPLIQWTMNQCLVEIAVAYPDYLEQGLVIGQELAVYSDMKVPKGCTSAYAPDWIE
                     ALLRRK"
     gene            complement(6948..8435)
                     /locus_tag="U1099_001352"
     CDS             complement(6948..8435)
                     /locus_tag="U1099_001352"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEM4867671.1"
                     /translation="MSSHVLFTIFGASGDLAKRKLYPSLFRLYKAGHIKENFAVIGTA
                     RRPWTKEFFEQTVIESLGDLPDTPRQAHEFASHFYYQSHDVNDTEHYVALRKLQDDLC
                     EKYNTQHNKVFFLSMAPEFFGTIAKHLKSEQIVDGQGFERLIIEKPFGTSLETASKLN
                     DELAAAFNEDQIYRIDHYLGKEMVQNIFAVRFANIIFEHIWNRDYIDNVQITFAEAIG
                     VEDRGGYYDHSGALKDMVQNHALQVLSLLAMDKPASFKEEDVRAEKIKVFQHLRQPSD
                     EDLKRNFIRGQYAAGSIDGKDYVSYLDEPNIAEGSQTETFAGGVFFVDTDRFRDVPFF
                     FRTGKRLTEKGTRVTITFKHAEDIFGQPSEANVLTIFIQPTEGFMLSINGKEVGSHFA
                     LTPAKLNFRHNATALGNSPEAYEKLFFDVLNGDSTNFSHWEEVKASWSLIDRIVDLWA
                     SNQVPLHTYPAGTMGPEATFDLLESYGCKWVWTPDVWYRERGLLK"
     gene            complement(8514..9329)
                     /locus_tag="U1099_001353"
     CDS             complement(8514..9329)
                     /locus_tag="U1099_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEM4867672.1"
                     /translation="MIRFLILAGYFEMTMYLYISGKLDQYINLHYSYLAYLSMVLSFI
                     LAIVQLYVWVKEIRVHSHLKSKIAKLSSVGLLLIPLAIAWLFPTVSLDSTTVAAKGYH
                     FPLAAENDTNTQNQEGTTVQYLKPDTSIYFTKSAYQNQMREIADRYLAEETIIVTNEN
                     YMEVMEAIYDYPTEFSGKTIEMIGFVYNDPDNSQQFFLFRFGIIHCIADSGVYGLLST
                     GATTEFPNNTWVKARGTIKVSYHTSLKQNLPTLELQSMVQIEQPDSPYVYRVF"
     gene            complement(9326..10231)
                     /locus_tag="U1099_001354"
     CDS             complement(9326..10231)
                     /locus_tag="U1099_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEM4867673.1"
                     /translation="MFGLDQLPTSVLQAGAIFLSIMIEALPFVLIGAIISGFIDVFIT
                     PDKVRKLLPKNKFLAILFGTFIGFIFPSCECGIVPIVNRLLEKKVPSYTAVPFLVTAP
                     VINPIVLFATFTAFGNSLLFAFYRALGAIIVSLVLGIILGFFVKGPILKGSKFDHHQH
                     GYSDKTSWQKVGFALIHAIDEFFDTGRYLVFGCLFASLVQVYIPTAVLTTIGHSPLTA
                     ILLMMLLAFLLSLCSEADAFIGASLLSSFGFAPVMAFLVIGPMVDVKNLLMMKHYFKG
                     KFIVGFIITITLVILGYSLILGGVV"
BASE COUNT         3083 a         1964 c         2462 g         2856 t
ORIGIN      
        1 gttggatttg accaagttaa tcagtgattt ggagcgaaaa attgaggtcc ataaactgga
       61 atctagtcaa aacgaatcaa gtcgtcaaga agcgcaggcg cgcttggaaa acttgttgac
      121 tagacgagag cagttggcag aacagattga gcaaaagcaa gaaacgcttg cgcaattaga
      181 tagttccttg tctagcttga aagacgatat tgctgcggta gacaaagaaa tttcctactt
      241 ttcagaagat cctgatcagg ttttagatca tctgcgtgaa cagtatgttg ctctgatgca
      301 ggaagaggca gaagcttcaa acagtctgac caagattcag caggatattg ccaatcaaat
      361 cagtctttct gagagtaaat ctgaagactt ggcacgcttg cagacggaaa aacaaacagc
      421 tcaagaagta cttgataaga gtcggaaaag tttagaagaa gcagatcacg ttttacgcca
      481 gttgcttgag agctatcaga ttaaaaaatc ggaattagat caaacccaga ctgcttatca
      541 ggctgagcaa ggtcgcttgt ttgatttgct ggatcagttg aaaggcaagc aggctcgcca
      601 gtctagttta gaagctattt taaaaaatca ctccaatttc tacgcggggg ttaaggctgt
      661 cttacaggcg gcaccaagct taggaggaat tattggagca gtcagtgaac aattgacctt
      721 tgatactcgt taccaaacgg ctttggaaat tgctcttggc ggtgctagtc aaaacgtcat
      781 cgttgaagat gaagcgattg ctaaacgagc tatcgcattt ttgaaagaaa aacggcaagg
      841 acgggcaacc ttcttacctc taacaactat caagccacgt cagctttctg gacagcaagt
      901 ctctttacta gaatcttcag aaggattctt ggggcttgca agtgatttgg tgacttatca
      961 gccaaattta gatgcgattt tccaaaattt gctaggcact attgcgattt ttgatagcat
     1021 tgaccatgcc aaccaggcgg cgcgtgcgac caagtttcaa gtgcgtatgg tgaccatgga
     1081 tggagcggaa atccgtcctg gtggagcctt tgctggtggt agcaatcgaa ataacagcac
     1141 gacctttatc aaaccagaac tggatgctct tttaggagaa atagcagagc tgtctagcca
     1201 gttgcaggag caagaaagct tggttgcagc taagaaaact agcttagacc aaaccagaga
     1261 agccttggaa acgattaagg cggaggggga agaagctaga ttgaaccagc agagcgcaag
     1321 gattcatcag gaacaggcag aaaatagact ggctcagcta tcagcccagt atgacctgca
     1381 aatgagtcag gttagtccga ccattttgac tgagctagaa gaggctgctg ctaaggaaga
     1441 agtaaatgtc caagccttga atgagaaaaa actagccttg gatcaacaaa tcaatcaagt
     1501 ccgtgataat cgcgacagta ttcaagaaag cttgcagaaa ctacagactc agaagggtca
     1561 actaactttg gaacaggctg aactgactag ccaacttcgt tttgaacaga cagatctgag
     1621 acgtttgcaa gaagaaaaag tggttgcaga taaggaaatt tctcttttag aggatatgat
     1681 tgaccagaag ctagaagctc ttgaagatac tagcattgag attcttgaag aacaattgca
     1741 ggccgcatct gataagcaaa accagaccaa tcagattcta attcgcttaa agtttgagtt
     1801 ggaagatatt gatgggcagt ttgaagactt ggaagaaaga ttgcaacaag ctcggactaa
     1861 gaatgatgat ttgatccgta agcaggctaa gttagaggcg gattgtgagc aagcgggcga
     1921 taaattacgg actttacttg gtaatttgac cgagcatttc aaattgagtt ttgaagctgc
     1981 tcagtctcag gccaaggaag ttgagaatct tgcagttgct gaacaaagcc tcaaagacct
     2041 agaaagagct attcgtgctt tgggtccagt caatttggat gctattgagc agtatgacga
     2101 ggtcaataac cgtctgacct tcctcaatga gcaacggaca gatattttgt cggcgcgtga
     2161 tttactacta gatactatcc acgagatgga tgatgaagtc aaagagcgct tcaaggttac
     2221 ttttgaagct attcgtgaaa gctttaagca gacattcaaa caaatgtttg gcggtggttc
     2281 agcggatttg attttgacag aaggtaacct attgactgcg ggtgtggaga tttctgttca
     2341 acctccgggt aagaaaattc aatctctcaa tcttatgtca ggtggcgaaa aagccctttc
     2401 ggccctcgct ctgctatttt ccattatccg tgttaagacc attccttttg taattctcga
     2461 tgaggtggag gcagccttgg atgaggcgaa tgtcaaacgt tttggcgact acctcaaccg
     2521 ttttgataag gagagtcagt ttatcgtcgt gacccaccgt aaagggacca tggcggcagc
     2581 ggatgccatg tatggggtga ccatgcagga atctggcgtg tccaagattg tttccgttaa
     2641 gctgaaagat gtggagaaat tatgattaac aaaatttacg ccagtgatat ggatggtacc
     2701 tttttacgag aagaccatag ttttgataag gaacggtttc gtcgtatact gaatcagttt
     2761 aaagaaaaag gttatttatt tgtggcaaca agtgggcgat ctatgcaaag cctgaaactt
     2821 gtttttgaag attttgtaga tgaaatcggt tttgtggcag aaaatggttc aatcgttgaa
     2881 tatcaggggc agactatctt tatggatgat ccaattagtc ctgaaattta tctgccaatt
     2941 atcgcgggta ttgatgcagg tccctttggt agcagtcgtt ccatggtgct gtctggactt
     3001 gaaaactttt atttattaaa aaatgctgag cctcagtttt tagaagcgat gacgaattat
     3061 tacgcacatt ttcgattggt agacacattt gaagaagttc gagaagagat tataaaaatc
     3121 aatgccaaat tttctcctga ggaaatggac gatgctagac gatggttaaa tgatacattt
     3181 gaaggtgtaa cggccatgac aactggtttt gacaatattg atataattcc aaatggttca
     3241 aataaatcag ttggattgag tcatctctgt gctcattttg gaattacccg tcaggatgta
     3301 gtggcatttg gtgataatca aaatgatttg gatatgtttg actttgcagg tctggctctt
     3361 gcgacagaaa atgcaagaga ggaagtgaag gcccaggcag attggatgat tggtcattgc
     3421 aatgatggag cagtattagc ctatttagag gaggaagtaa atggcaatta agttgattgc
     3481 attggattta gatggaacac ttctgacatc ggacaaacgg atttcagaag ctaataaaaa
     3541 ggccttgcag gcagcgcgtg agcgtggagt ttatgtagtt ctgacaacag ggcgtccttt
     3601 acaagctatc ggtacttttc tagaagaact agatctgcta ggggagaatc aatattcgat
     3661 tacatttaac ggtggtctag tccaggaaaa tacaggtcgg gttttggata aggttggctt
     3721 caccattgaa gatgtgcgca ccatccgtcg tgtgacaaat gatttggacc tgcctttgga
     3781 tgtcctgtat ggtggcgatg tctattctct accatcagcc catgaatcac tttatctaac
     3841 ttgtaatcca ttgcttaata aaattgtggc aagcgatgag gaaatgccag aagattttgt
     3901 ctacaataag gcagtttcag ctgtggcagc agactttttg gatggacaaa ttccaaaaat
     3961 tcctgcggaa cttcacgagc gtttcgagat tttcaaatcg cgtgatattc tgctcgaatg
     4021 gagtccaaaa ggtgttcata aagcaaatgg tttggctaag ttgattggac atctggggat
     4081 tgatcagtca gaagttatgg cctgtggcga tgaaggaaat gatctttcga tgattgaatg
     4141 ggctggtctg ggtgttgcca tggctaatgc gactgatgaa atcaagtctg cggccaacct
     4201 tgtcctgcca aaaaacaatg atgaagatgg cattgcttgg gtcatcgagc actatgtatt
     4261 gaatgaggac tagtatggga ttatttgatc gattatttgg acaaaaaaaa caagaggagc
     4321 aagtcgctct tgttgaagaa gtggaaaggg agcagtcggc ttctagcgaa gatacagtaa
     4381 ctactgaagt tgagtcagct actcaagaaa tggaaaagtt ccagtctgaa gtagcagaga
     4441 ctgaattgac agccgaacaa gaagctcaga aacaacggac cttagacatg atggctcaat
     4501 attatgcagc caaagaagcc gctgcggctc gagtgaagga agcgcaggaa caaggctttg
     4561 accctgctat tcaaaccaag gtcaaggaag agcctgttga ggaagttgct ccagtagggc
     4621 aagaaagtga acaggacaag taccagcgaa ccttgaaaaa aactcgtaca ggatttgggg
     4681 caaggctcaa tgaattcttt gccaatttcc gttcggttga tgaagaattt ttcgaagagt
     4741 tggaagaaat gctgatttta tcagacgtag gtgtgcaagt tgcttctacg ctgacagagg
     4801 agctccgcta tgaagccaag ctacaaaatg ccaagaagac agatgaactc cgtcgggtta
     4861 tcatcgaaaa attggtggat atttacgaaa aagatggtca attcagcgag cgaatcaatt
     4921 tccaagatga tttgacagtc atgctctttg tcggtgtcaa cggtgttggg aagacgacgt
     4981 ctatcggtaa gttagcctac aagtacaagc aagctggcaa gaaggtcatg ttggtcgcag
     5041 cggacacctt ccgtgcgggt gcggtagccc agctggtcga gtggggtcgc cgtgtcgatg
     5101 tgccagtagt cacaggacct gaaaagtcag acccagctag tgtggttttt gatggggtaa
     5161 aacgtgccgt ggcagaaggc gtggacattc tcatgattga cacagcaggt cgcttgcaaa
     5221 ataaagaaaa ccttatggca gagctggaaa aaatcggtcg catcatcaaa cgaaccctac
     5281 ctgatgcacc gcatgaaacc ctcttagcac tagatgcctc cacaggtcaa aacgccctca
     5341 gtcaagccaa ggaatttgca aaaattactc cattgactgg gctagtcttg actaagctag
     5401 acggtacagc aaaaggtggt gttgtccttg ccatccgtca ggaactggat attccagtca
     5461 agctgattgg tttcggtgaa aaaattgatg acataggtga attcaaatcc gaagaattca
     5521 tgcgtgggct tttggaagga ttggtataga aaaaagtcgt tagttcaatg aactagcgac
     5581 tttttctatc tgtatgcaac agtgagtaaa tggcaatatc cacaataccc cgattatttc
     5641 gagcggcttg gcggagagtt ccttcatagg tcataccgac tttttccatg acacgaccag
     5701 aaggacgatt ttcactatcg tgaacagctg taatccgtaa acagcccact tcctccaata
     5761 aatagtgatt aacagcttca agagcttcgg ccatataact ttgcccccac catttactgc
     5821 ccaacaccca acctaattcg gcagcttgaa ctctttcatc aaggctgaca acggaaatat
     5881 ctccaatggg ttgctgattt tctttgagct caattaccca cctgtatgtt ttttcctgtg
     5941 aataggattg aagacagaac tggacatagt cttcactatc ttggagtgtc tgatgagttg
     6001 gccaggtcag ataggtggta accttttcat ctgaagtcca gttttggaaa actgattcaa
     6061 catcgcttgc ttgaaaaggg cgtaaaatta gacgttctgt ttctaaaatc tgagttcctt
     6121 tatgttgcat cttatttcct cctcaacaat gcctcaatcc agtctggtgc ataggcagaa
     6181 gtacagcctt ttggcacctt catatcggaa tagacagcga gttcctgacc gattacaagt
     6241 ccctgttcca ggtagtcggg ataggcaacg gcaatctcca ccaagcattg gttcatggtc
     6301 cattggataa gcgggtctgc ggtttgtaaa tgctggcgaa tatggtctag agtttcttca
     6361 atttcttggt tttttgctgt ttttccagca aagtatttgg cacgaaggaa ccaacctagg
     6421 cgttgaaaaa tagcttggtc tttggcaagc aaatcttctt cccaaaccct gctgtctttt
     6481 cgtttgctga gaatgatgga agccagctta tcgagcacat tgaccgaaat ttgttcattg
     6541 aggcaattct ctaggtcagc ttggggaagc tggtcaggct tattttttgc aatttgaata
     6601 gctaaatatt gtgcttcaag gacacctgtt tgccagagtg gggcaaataa gtccagtcga
     6661 tttttatagg ccttggcgat accagaaata gctcccattg gaacgccgta gtagggctta
     6721 gtttcgccaa ttttttcata acgcttgagg gtgccagcat ggctggctgc ttggagttgt
     6781 gtgagaatgg tgtttaattc agtcataaat atctcccaaa aaaactggat tgctccagtt
     6841 tttatatgta gtcgtttagt ttgcttttag tacaaggcaa cgagctgtag gctgtactaa
     6901 agtacggcaa agcgagttaa cgaagtaatc aaagataaac taaatgacta tttcaataag
     6961 ccacgttcac gataccatac atctggtgtc caaacccact tgcaaccgta gctttctagc
     7021 aaatcaaagg ttgcctcagg tcccatagtt ccagctggat aggtgtggag tgggacttgg
     7081 ttgcttgccc acaaatcaac aatgcggtca atcaagctcc agcttgcttt cacttcttcc
     7141 caatggctaa agttggtaga gtcgccgttc aagacatcga aaaatagttt ttcgtaggct
     7201 tcgggtgaat ttccaagagc agttgcattg tggcggaagt tgagtttggc aggagtaaga
     7261 gcaaagtggg aaccaacttc cttgccgttg atagagagca tgaagccttc tgttggctgg
     7321 atgaagatgg tcaatacatt ggcctcagaa ggctgaccaa aaatatcttc cgcatgcttg
     7381 aaggtgatgg tcacgcgcgt gcccttttct gtcagacgtt tacctgtacg gaagaagaaa
     7441 ggtacatcac ggaaacggtc agtatcaacg aagaagacac ctcctgcaaa ggtttctgtc
     7501 tgagaacctt cggcaatatt tggttcatcc aagtagctaa cgtagtcttt tccgtcgatg
     7561 gaacctgctg catattgacc acggatgaag ttgcgtttga ggtcctcatc agaaggctga
     7621 cgaaggtgtt ggaagacctt aatcttttca gcgcggacat cttcctcttt gaagctggct
     7681 ggcttatcca ttgctagaag ggataggact tgaagggcat ggttttgcac catgtctttt
     7741 aaggcgccag aatgatcgta gtagccccca cgatcctcaa caccaatcgc ttcagcaaat
     7801 gtaatttgga cgttatcgat gtaatcgcgg ttccaaatat gctcaaaaat aatattggca
     7861 aagcgaaccg caaagatatt ttgtaccatt tccttgccta ggtaatggtc gatacggtag
     7921 atttggtctt cattgaaggc agcggctaat tcatcattga gtttgcttgc agtttcaagg
     7981 cttgttccga aaggtttttc gataatcaaa cgctcaaaac cttgtccatc aacgatttgc
     8041 tcagatttga ggtgtttggc aatggttccg aaaaattcag gcgccataga gaggaagaag
     8101 accttgttgt gctgggtatt gtatttttca cacaagtcgt cctgcaattt acgaagagct
     8161 acataatgtt ccgtgtcatt gacatcgtgg ctttggtagt agaaatgact tgcaaattca
     8221 tgagcttgac gtggtgtgtc aggcagatca cctagtgatt caatgacggt ttgctcaaaa
     8281 aattccttgg tccagggtct gcgagctgta ccaattactg caaagttttc cttgatgtgt
     8341 ccagccttat agaggcgaaa taaagatggg taaagtttgc gtttggcaag gtcgccacta
     8401 gcaccaaaaa ttgtaaacaa tacatgtgat gacataattt cattcctatc tgttgaatat
     8461 cattaatagt ataccacaaa ttgtcagaaa attcactttt tgagttggaa ttattagaaa
     8521 acacggtaga catacgggga atctggctgt tcaatctgaa ccatcgattg taattcaagt
     8581 gttgggagat tttgtttcaa cgatgtgtgg taggaaacct tgattgttcc tctggctttt
     8641 acccaagtat tgttaggaaa ttctgtggta gcccccgtag agagtaagcc atatacccca
     8701 gaatcagcaa tgcagtgaat aataccaaag cggaaaagga aaaattgctg gctattgtca
     8761 gggtcattgt agacaaatcc aatcatttca atcgtttttc ctgaaaattc cgtaggatag
     8821 tcgtagattg cttccatcac ttccatatag ttttcattag taacgataat ggtttcttca
     8881 gccaagtatc tatcagcaat ttcacgcatc tggttttgat aggcagattt tgtaaaataa
     8941 atagaagtat caggcttgag atactgaaca gtcgtccctt cttggttttg tgtattagta
     9001 tcgttttctg ctgctagtgg gaaatgatag cccttggcag caacagttgt tgaatccagg
     9061 cttaccgtag gaaagagcca ggctatagca agaggaatga gcagtagtcc aacgcttgat
     9121 agtttggcaa ttttgctttt taaatggcta tgtactcgaa tttcttttac ccagacatac
     9181 aattgtacga tggcaagtat gaaagataaa accatggata gataggctag atagctatag
     9241 tgcagattaa tgtattggtc taattttcca gagatataaa gatacatggt catttcaaag
     9301 taacctgcaa gaattaaaaa acgaatcata ctacacctcc aagtatcaga ctatatccca
     9361 aaataacgag agtgatagta atgataaaac cgactataaa tttccctttg aaataatgtt
     9421 tcatcatgag caagttttta acatccacca tgggtccgat aactaagaaa gccattactg
     9481 gggcgaatcc gaagctggat agtagtgatg caccaataaa ggcatcggct tcggaacata
     9541 gggaaagaag aaaggccagc aacatcatca gtaaaatagc tgttaatgga ctgtgaccga
     9601 tggtagtcag gacagcggtt ggaatataga cttgtacgag actggcaaat agacaaccaa
     9661 agactaggta acgtcccgta tcaaaaaatt catcaatggc atgaataagt gcgaagccaa
     9721 ctttttgcca actggtttta tctgaataac catgctggtg atggtcaaac ttgcttcctt
     9781 taagaattgg tccttttacg aaaaaaccaa gaatgattcc caatactagg gatacaatga
     9841 tggctcctag ggctctatag aaggcgaaaa gtagggaatt tccaaaagcg gtaaaggttg
     9901 caaaaaggac gataggatta atcacaggcg ccgttactaa aaagggaaca gcagtataac
     9961 tcggcacttt tttctctaat agccgattga caatagggac aataccacat tcgcaagacg
    10021 gaaaaatgaa gccgataaag gttccaaata gaatggcaag aaacttgttt ttaggtaaga
    10081 gtttacgaac cttatctggt gttataaaca catcgataaa acctgaaata atagctccaa
    10141 tcaagacaaa gggcaaggct tcaatcatga tagacaggaa aatagccccc gcctgtagga
    10201 cggaggtagg taattgatca agaccaaaca tattattttt tatcctgatt caagttgttt
    10261 ttctcggaat tttcttttga ttttggatct ggatactcaa tttcttccaa tttttcaaat
    10321 gaagcaaatt cttccagcat cttttctaaa tcatcttgtc gttta
//
LOCUS       DASFSW010000075        10316 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.075, whole genome shotgun sequence.
ACCESSION   DASFSW010000075 DASFSW010000000
VERSION     DASFSW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.075"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..662)
                     /locus_tag="U1099_001355"
     CDS             complement(<1..662)
                     /locus_tag="U1099_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEM4867674.1"
                     /translation="MPNYTIGILGLGVFGTTIAKTLHNYDCNIIAIDNHEQRINHLEP
                     ILTRGIVGDITDRSLLRAAGIGNCDAVVVATGENLESSVLAVMHSKVLGVPMVIVKVK
                     GTTAKEVLLRVGADKVISPERETGISLAKQLLHRDTTSLFELEGNVSIVEFHPPVKWI
                     GKTLGELKLRQHYKLNIIGYRSGENQELNIQLTPDYVFKSDELILAVTDHNTVDHFEE
                     LT"
     gene            complement(674..2023)
                     /locus_tag="U1099_001356"
     CDS             complement(674..2023)
                     /locus_tag="U1099_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEM4867675.1"
                     /translation="MSPFRIKLSPSQRIIISFLFVILTGSILLSLPISQISTSTASYW
                     DHLFTSVSMVCVTGLFTKAVAETYSTFGQIICMMLIQIGGLSLIGFIGLFALRGGRKM
                     NFINMATLQEALNRSDTKYFRNFLKSAFGFTLAVEAFGALLLSFHFVPEFGWARGLFT
                     SIFVAISAFCNAGFDNFGATSIMRYVDNPLINITLASLIIMGGLGFSVWFDLQTQIGQ
                     KRSLRKLGFHTKIVLGLTAIILTLGTLTTLLTEWNNPSTIGQLSFGHKLLASFFQTVT
                     MRTAGFASIDYTKAEPITLLLYIFQMMLGGAPGGTAGGIKITAFLTLVLYARSEILGL
                     PHTNFMGRTIDNLSIRKAFATFSVFLLMFIIGLFALALTDDRQPLLFLMFEVMSALAT
                     VGVTANLTPSLTMAGQAVIMALMFFGRIGPLSILASLSVRKLSKTESLQYAKSSMLV"
     gene            complement(2287..2826)
                     /locus_tag="U1099_001357"
     CDS             complement(2287..2826)
                     /locus_tag="U1099_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEM4867676.1"
                     /translation="MNSKRQTLNFITMLAAQAVVISLIERLITPPFAFAPGAKLGLGN
                     LISLIAIFTLPTKDSLKVVALRLLISTFLGGTFSTFLYGFAGTTLSYVGMLSAKQLGP
                     NRVSPIGISILGGMLHNLGQLLVFATIARSFYVLNYLPILAFTGILSGLLVGLAATYL
                     LQKVGPLRHYHQLVLAEWK"
     gene            3009..4901
                     /locus_tag="U1099_001358"
     CDS             3009..4901
                     /locus_tag="U1099_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM4867677.1"
                     /translation="MTKNIVIVGAGYAGIAAARLLGKTFKKDQDVTVTLIDKNSFHTY
                     MTELHEVAAGRVEANAIKYDLQRIFKKYPKVQLVTDKVVEIDYDKKQVVAEHQTLDFD
                     YLLLAMGGEANDFGVKGVKEHGFTLWSIEAAERLHDHMIDACYRAMREHDEAKRRALL
                     TFTVIGAGFTGIEMIGELIDWVPILAREFKLDPKEFSLKVVEATPNILAMVTEKEQVK
                     ARKYLEKKGVELVLGDGVASVQEDSLTLSSGRQIPTYTSIWTAGVQANTDASEFGIEK
                     ARAGRLVANEFMEAKGKENVYVAGDLVYFEESEGKPTPQIVQAAEQTGHTAARNIISA
                     IKGGEKHSYKGKYDGFMVSIGARYGVAFLMDKYHMSGFMAMAVKHMVNLLYFFTIRSF
                     FYMGSYVRHEFFDIQNKRNIFGGHTSGKGNLLWSVPMRVLYGSVWLYEGIKKAFGLFG
                     TTSWFGDQVVFPFPWLADPVSGASAAEAVSSASQAATAAAEAAEPIFGLSYAYGEAPM
                     AVLDKMPDWFATIMEFMMPNQEVALFMQKFMTVAEIGIGLALIAGAFVWIVSAATVAL
                     VVMFSLSGMFYWVNIWFIPAAISLMNGAGRAFGLDYWIMPWLGRFLDKKIYGKPKHIY
                     RIKYKK"
     gene            4920..5909
                     /locus_tag="U1099_001359"
     CDS             4920..5909
                     /locus_tag="U1099_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM4867678.1"
                     /translation="MVHQIWNTYPEIERGLEEVKSIILSEMMVLHPAVTSKIVEYVEA
                     PGKYLRAGLCLLFAQMTEGKISRSKLYFAAHLEVLHLATLIHDDVIDGADKRRGVTAA
                     HQQFSNRIAIYTGDYLLAYSSRLFGKGLRLLEVSRDDLDLGNDKLMETILRGELSQLL
                     NQFDANMTMKAYLKQIQGKTAFLFGLACQLGSFVPGQSRKDSSMAFRSGQALGMAFQL
                     RDDLIDYQLDVAESGKPRLQDIQNGIYTAPLLFAMREDKSIQQQLRAYIQSPTAEGLE
                     ILTDRIFATHAIAKTEKLIVSYLEKMMNFVDRIPQLSTASMQNLVAIVFSHYF"
     gene            5988..6467
                     /locus_tag="U1099_001360"
     CDS             5988..6467
                     /locus_tag="U1099_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEM4867679.1"
                     /translation="MKTTKVVLKSLVVLGAVFALAACGSKQESASTSSSTKETVVLKD
                     GTYKAESSKDDYGYKIVHTLTVKDGKISESKFDYEAEDGSLKSANEEYNKKMKDKAGV
                     SAGEAIEQLNAALVEKQELSAVEVVSGATHTSEDFKKSTEALLAAAAEGKTETVKLD"
     gene            6545..7594
                     /locus_tag="U1099_001361"
     CDS             6545..7594
                     /locus_tag="U1099_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM4867680.1"
                     /translation="MKKIASLLFVGLLMVLLVACGGKQNESLSVVKTPLTRSESLLHT
                     VVQLSIYHENQEEAMDEAVSYIKEMESLLSTNLEGADVYRINQAAGKEAVKVDERTFE
                     VIETAIDMSKESQGLFDISIGAVSNLWKIGDEDARKPSDEEIKAALPFINYKDITLDK
                     EKKTVFIKEGMTLELGAISKGYIADKVRELFASKGITTAIINLGGNVVVMGDSPTTQN
                     GWNVGVQDPDQVRGSTVGSVPGTHDSVVTSGIYERYLEVDGVKYHHILDPKTGYPVEN
                     DISGVTVFSKTSIQGDALSTTLFLLGVEKGLDFINQIDGVEAVFIDKEQGVHLSDGLK
                     ESFELTNEEYHLVNE"
     gene            7587..8543
                     /gene="menA"
                     /locus_tag="U1099_001362"
     CDS             7587..8543
                     /gene="menA"
                     /locus_tag="U1099_001362"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEM4867681.1"
                     /translation="MNKSTKGLSLPVFLEFVEMKTKVASVFPMTLGILWSLYRYQAFN
                     WLNTLLFIIAVLSFDMCTTAINNSMDYHKAKDEAYRQESNVIGKFSLDFRQMIGIVLA
                     LLIFSLLVSLLLVWRTSWLLLPMGALCFLIGIFYTFGPIPLSRMPLGEVFSGVTMGFG
                     IFFLAVFIQQPDLLLTSQVSGQWMTLQFSWIKIIEIIFMSIPLVTLIANIMLANNTCD
                     LEEDIRNHRYTLVYYIGKKNALKLYFVLASLPWLLWIIYCLIGFLPIWALAGLIGVWP
                     AYKSLETFLKKQVKRETFIEAVKSFVLFALLYVVILALALIF"
     gene            complement(8851..9279)
                     /locus_tag="U1099_001363"
     CDS             complement(8851..9279)
                     /locus_tag="U1099_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEM4867682.1"
                     /translation="MTFKSILKQLKLFDYILIGFTLVLSFLPAIFTYTHLTTDTNEAK
                     TIAYVRINGEVVDQFELSKDTPHQEKTYYPNEGQYNIIEVDGERIRVKEDNSPDQIAV
                     MTSWISQPGQLSVCLPHNLLIEIKSVGGESTDEEELILPL"
     gene            complement(9332..9679)
                     /locus_tag="U1099_001364"
     CDS             complement(9332..9679)
                     /locus_tag="U1099_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867683.1"
                     /translation="MNVDKPKFILTLIVLSYTAICSLYIHSYLTGDNPPIFILLFTML
                     PILNSDWQTSGPEANAFQKAIYLYHKPLLWFLFAGFALTFSIVFFAGKIQPLHAGFWG
                     MLAILLFFRQLKK"
     gene            complement(9669..10013)
                     /locus_tag="U1099_001365"
     CDS             complement(9669..10013)
                     /locus_tag="U1099_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM4867684.1"
                     /translation="MNKPFTPKQTCRFFVLFYALFSAFFIYSYMVNEPINGSFLLMLP
                     TIQIIKSYQTAEEKRYFIIQAIFALFAMTLTYSVGIWLGHVKPLILILPAGFAAAILY
                     RIYFKKEKPDER"
     gene            complement(10026..>10316)
                     /locus_tag="U1099_001366"
     CDS             complement(10026..>10316)
                     /locus_tag="U1099_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867685.1"
                     /translation="MRTLHADLPQYDMDELSPEEKQGQTLNPLVLGLGVALLSSLFAG
                     LADLFSLQLPLLASIFHVKSLLFGGMMGLFASLALSAIAYLQKLEADSVKKK"
BASE COUNT         3073 a         1847 c         2412 g         2984 t
ORIGIN      
        1 tttgtcagtt cttcaaaatg atcgacagtg ttatggtctg ttactgctaa gatgagttcg
       61 tcagatttga agacatagtc aggagtcagt tggatgttta attcctgatt ttcgccactt
      121 cggtaaccga tgatattgag tttataatgt tggcggagtt ttaattcgcc gagtgttttt
      181 ccaatccatt ttactggcgg gtgaaactcg acaatggaaa cattgccttc gagctcaaag
      241 aggctggttg tatcgcggtg gagtagttgt tttgctagag aaatacctgt ttcacgttca
      301 ggagaaataa ccttatcggc accgacacga agaaggactt ctttcgcagt tgttcctttg
      361 actttgacaa tgaccatagg aacacctaga actttgctat gcatgacagc cagaacactg
      421 gattccaagt tttcgccagt agctacaaca acagcatcgc agttaccgat tcctgctgca
      481 cgtaggagcg agcgatcggt gatgtctccg acaataccgc gtgtgagaat aggttctaga
      541 tgattgatac gttgctcatg attatcgata gcaatgatat tgcaatcgta attgtgtaat
      601 gtttttgcaa tggtagtacc aaaaactccc aggccgagaa ttccgatagt atagtttggc
      661 atgatttctc cttttaaaca agcatagatg attttgcgta ttgaagactt tcggttttag
      721 ataattttcg aacagaaagg cttgcgagaa tagaaagtgg accgatacgg ccgaagaaca
      781 tgagcgccat aatgactgct tgaccggcca tggttaggct tggagtcaga tttgccgtaa
      841 cacctacagt ggctaatgct gacatgacct cgaacattaa aaatagaagt ggttgtcggt
      901 catctgttag ggcaagtgcg aagagcccta tgataaacat aagtaggaag actgagaatg
      961 tcgcaaaagc tttgcggatg ctgaggttat cgatggttcg tcccataaag ttggtatgag
     1021 ggagaccgag aatctcgcta cgagcataga gcactaaggt taggaaggcg gttattttaa
     1081 taccgccggc tgttccacct ggggctccgc caagcatcat ctgaaagata tagagcaaga
     1141 gggtgattgg ttcagccttc gtatagtcga tgctggcaaa accagcggtt ctcatggtga
     1201 ccgtctggaa aaagctggct aataatttat ggccaaatga tagttgcccg atagtagaag
     1261 ggttattcca ttcagtgagt aaggttgtca gagttccgag ggttagtata atggctgtta
     1321 ggcctagaac aatctttgta tggaacccta gttttcgtag gctacgtttt tgcccgattt
     1381 gtgtttgaag gtcaaaccaa accgagaatc caagacctcc cataataatg agactggcta
     1441 atgttatgtt gattagagga ttatccacat agcgcatgat gctagttgcg ccaaaattat
     1501 caaaaccagc attacaaaag gctgatatag ctacgaaaat ggaggtaaat aaacctctcg
     1561 cccatccaaa ttcaggcaca aaatgaaagg acagtaaaag ggctccaaag gcttcgaccg
     1621 ccaaggtaaa accaaaagct gatttgagga agttacgaaa atattttgtg tcagatcggt
     1681 ttagagcttc ttgtaaggtc gccatattga tgaaattcat tttacgacca ccgcgtaaag
     1741 cgaagagacc gatgaatcct atcaagctca aaccaccaat ttggattagc atcatacaga
     1801 taatctgtcc aaaagtagaa taggtttcag caacggcttt ggtaaatagc cctgtcacac
     1861 aaaccatgga gacagatgta aagagatgat cccaataact tgctgtagag gtgctaattt
     1921 gtgagatggg aagacttagt aaaattgatc cagtcagaat cacgaataag aaactgataa
     1981 tgattcgctg actgggagaa agtttgatcc taaatggtga cataattcta ccttttctca
     2041 aaaaaataaa agatgaagat attgtagcac aaaagaaaga aaatgtacga ctaaaacacg
     2101 caaagaacct gatttttttc tcttcttgaa agcttcatta ttttttatta ccatggattt
     2161 tataagtggt gtttatatga ggtggctcat tatatttgtg acaccaggat tatggggaaa
     2221 ttattatggg caatgaaaaa gtggtgcgga ccataaaaag aatctcctta gttgacctag
     2281 ggagatttat ttccattctg ctaagactag ttgatgatag tgacgtaaag gtccgacctt
     2341 ttggaggagg taggtagcag ccagtccgac caataagcct gataaaattc ctgtaaaagc
     2401 gaggattggc aggtagttta gtacatagaa agaacgggca atggtagcaa aaacaagaag
     2461 ctgaccaaga ttgtgcaaca tcccgcccag aatagaaatt ccaataggac tgacacggtt
     2521 tggaccgagc tgcttggcag agagcattcc tacataactc aaagtagttc cggcaaagcc
     2581 atagaggaat gtggaaaagg tgcctcccag aaaggttgag atgagcaagc gtagagcgac
     2641 aacctttagg ctatcctttg tagggagggt aaagatagca attaagctaa tcaaatttcc
     2701 cagtcctagc ttggcgcctg gagcaaaagc aaatgggggg gtgatgagtc gttcaatcaa
     2761 agaaatgaca actgcctgag ctgctaacat cgtaataaag ttaagtgttt gacgtttaga
     2821 attcatgggt gtagagaaaa aactctttct agtcgattat ctattttact atcatacttg
     2881 tttttgttca ctatggcaag ttttgtttgt gtatattact aattattgaa atttgcttgt
     2941 caactatctt ggaatacgat ataatggaaa catgtaattt gttttgattt tcacaaggag
     3001 gaaacattgt gacgaaaaat attgtgattg tcggtgctgg ttatgcgggg attgcagcag
     3061 ctcgattgct aggaaaaact tttaaaaagg atcaagatgt aacagttacc ttgattgata
     3121 agaactcttt ccatacctat atgacagagt tgcatgaagt tgcggctggg cgtgtggaag
     3181 ccaatgcaat caagtatgac cttcaacgta tcttcaaaaa atatccaaag gttcaattgg
     3241 taacagacaa ggttgttgag attgactacg ataagaaaca agtagttgcg gaacaccaaa
     3301 ctctggattt tgattacctg ctccttgcaa tgggtggtga ggctaatgac tttggtgtga
     3361 aaggtgttaa agaacacggt tttacacttt ggtctatcga agcagcagaa cgtttgcatg
     3421 accacatgat tgatgcttgc tatcgtgcca tgcgtgagca tgatgaagcc aaacgtcgcg
     3481 ctcttttaac ctttaccgtt atcggtgctg gcttcacagg tattgagatg attggtgagt
     3541 tgattgactg ggtaccaatt ttggcgcgtg agttcaagtt ggatccaaaa gaattctcac
     3601 ttaaggttgt tgaggcaacg ccaaatatct tggctatggt tactgaaaaa gagcaagtca
     3661 aggctcgcaa ataccttgag aaaaaaggtg ttgaattggt tcttggcgat ggtgtagcaa
     3721 gtgtacaaga agatagcttg acactttcat caggtcgtca aattcctacc tatacctcta
     3781 tttggacagc tggtgttcaa gccaatacag acgcaagtga atttggtatt gaaaaagcac
     3841 gtgcaggtcg tttggtagcc aatgagttca tggaagcgaa aggcaaggaa aatgtctatg
     3901 tagctggcga cttggtttac tttgaagaat cagaaggaaa accaactcca cagattgtac
     3961 aagcagctga acagacaggc catactgcgg caagaaatat tatttcagca atcaagggtg
     4021 gcgaaaaaca tagctacaaa ggtaaatatg atgggtttat ggtatctatt ggagcacgct
     4081 acggtgtagc cttcttgatg gacaaatacc acatgtcagg ctttatggca atggctgtca
     4141 aacacatggt aaacctactc tacttcttta ctatccgtag tttcttctac atgggttctt
     4201 atgttcgtca tgaattcttt gacattcaga ataagcgaaa catttttggc ggtcatactt
     4261 caggcaaagg aaatcttctc tggtctgtgc caatgcgtgt cctttatggt tcagtatggc
     4321 tctacgaagg tatcaagaaa gcctttggtc tatttggaac aacatcatgg tttggggatc
     4381 aagttgtctt cccattccct tggttagctg atccagtatc tggtgcatct gctgctgagg
     4441 cggtttcaag tgcctcacaa gcggcaactg ctgcggcaga agcagctgaa ccaatttttg
     4501 gtttgagcta tgcctatggt gaagcaccga tggctgtttt ggacaaaatg cctgattggt
     4561 ttgctactat catggaattt atgatgccaa accaagaagt agcactcttt atgcaaaaat
     4621 ttatgacagt agcagaaatc ggtattggtc tggctttgat tgctggtgcc tttgtatgga
     4681 ttgtatctgc tgcaaccgtt gctttggtag ttatgttcag cctgtcaggc atgttctact
     4741 gggtaaatat ttggttcatc ccagcagcta tttctcttat gaatggtgcg ggtagagcct
     4801 tcggtctaga ttactggatt atgccatggt tgggtcgttt cctagacaag aagatttatg
     4861 gaaagccgaa gcatatttac cgtatcaagt ataaaaaata aaggaaatat agaaagttag
     4921 tggtacatca aatttggaat acctatcctg agattgagag gggacttgaa gaggtaaaat
     4981 ccatcatctt atctgagatg atggtcctgc atccagcagt tacatcgaag attgttgaat
     5041 atgtagaggc gccagggaaa tacctgcgtg ctggcctctg tctgctcttt gcacaaatga
     5101 cggaaggaaa gattagccgt tcaaagctat attttgctgc ccatttagaa gtcctgcatc
     5161 tggcgactct tattcatgac gatgtgattg atggcgcgga taaacggcgg ggagtcacgg
     5221 cggctcatca acaattttcg aatcgaatcg ctatctacac gggtgattat cttctggcct
     5281 attcaagtcg actatttgga aaggggcttc gtttgctaga agtatcacgt gatgatttgg
     5341 atttgggcaa tgataagctg atggaaacaa ttcttcgagg ggaattatct cagctactca
     5401 atcaatttga tgccaatatg actatgaaag cctatctcaa gcaaattcaa ggaaaaacag
     5461 cttttctatt tggacttgcc tgtcagttgg ggagctttgt accaggccaa tctaggaaag
     5521 acagtagcat ggcttttcgg tctgggcaag cacttggcat ggcatttcag ctacgagatg
     5581 acttaattga ctatcagctg gatgtagcag aatcaggaaa acctagattg caagatattc
     5641 aaaacggtat ctatactgca ccgctacttt ttgccatgcg agaagacaag tctatccagc
     5701 agcaattgcg ggcttatatt caatccccta cggcagaagg gttggaaatc cttacagata
     5761 ggatttttgc aacgcatgct attgccaaaa cagagaaact gattgttagc tatttggaaa
     5821 aaatgatgaa ttttgtagat agaataccac agttatcgac tgcgtctatg caaaatttgg
     5881 tggcaatagt ttttagtcat tatttttaaa aaataactag acattttctc taattaggta
     5941 tatcattata agtataaaac tatttactta acaaaaggag cattgttatg aaaacaacta
     6001 aagttgtgtt gaaaagtttg gtagttcttg gtgcggtatt tgcactcgca gcttgcgggt
     6061 ctaaacaaga atctgcttca acttcgtctt caactaaaga aacagtagta ttgaaagatg
     6121 gtacttataa agctgagtca agcaaagacg attatggcta caaaattgtt cacacattga
     6181 ctgttaaaga tggaaaaatt agtgagtcaa aatttgacta cgaagcagaa gacggaagtc
     6241 ttaaatcagc aaatgaagaa tacaacaaaa aaatgaaaga taaagctgga gtttctgccg
     6301 gtgaagcaat tgaacaactc aatgcagctt tggttgagaa acaagaactc tcagctgttg
     6361 aagttgtatc aggtgcaact catacttctg aagacttcaa gaagagtaca gaagcccttc
     6421 ttgcagcagc tgctgaagga aaaacagaaa ctgtaaaact tgactaatta agtctataca
     6481 gaaaagacac agtcgcactt gtttggctac tgtgtcttct tattttttgg ggaaggtaaa
     6541 atcagtgaaa aaaattgcaa gtctattgtt cgtgggccta ctgatggttt tgcttgttgc
     6601 ctgtggtggt aaacaaaacg aaagtttatc tgttgtgaaa actccgttga ctcgtagtga
     6661 aagcttactt cacacagtag tgcagttgag tatttatcat gaaaatcagg aagaagcgat
     6721 ggatgaagct gtctcctata ttaaggagat ggaaagtctg ttgtccacaa acttggaagg
     6781 ggcagatgtc taccgtatca atcaggcagc aggaaaagaa gctgtcaagg ttgatgaacg
     6841 tacctttgaa gtgattgaaa cagctattga catgagtaaa gaaagtcagg ggctatttga
     6901 catttccatt ggagctgtga gcaatctgtg gaaaattgga gatgaggatg cacgtaagcc
     6961 gagtgatgaa gaaatcaaag ctgctctgcc atttatcaat tataaagaca ttactttaga
     7021 taaggagaag aagaccgtct ttatcaaaga aggaatgacc ttagaattag gagccatttc
     7081 caaaggttac attgcagata aggttaggga attgtttgcc agcaaaggga ttacgactgc
     7141 tatcatcaat ctaggtggga atgtagtggt tatgggggat tcgccaacga cacaaaatgg
     7201 ttggaatgtt ggagttcaag acccagacca agtgcgtgga tcaacggtcg gttctgtgcc
     7261 agggacacat gattcggttg ttacctcagg tatttatgag cgttacttag aggtggacgg
     7321 tgtcaaatat caccatatcc ttgatcccaa aactggatat cctgtggaaa acgatatctc
     7381 aggtgtgaca gtcttttcca aaacatctat ccaaggagat gccttatcca caactctctt
     7441 cttactaggt gtggaaaaag gtcttgactt tatcaatcag attgacggtg tagaagctgt
     7501 tttcatcgat aaggagcagg gtgttcatct gagtgatggc ttgaaagaaa gctttgaatt
     7561 aacgaatgag gagtatcatc ttgtcaatga ataaatcaac taaagggctc agtttgcctg
     7621 tatttctaga atttgttgaa atgaagacca aggtagccag tgtttttccg atgaccctgg
     7681 ggattttatg gagcctgtat cgctatcaag ctttcaattg gctcaatact ctcctcttca
     7741 ttattgccgt ccttagcttt gacatgtgta caactgccat caacaatagc atggactacc
     7801 acaaggccaa ggatgaagcc tatcgtcagg aaagcaatgt gattggaaaa ttctccctag
     7861 attttcgtca gatgattgga atcgtccttg ctctacttat tttttctcta ctggtttcat
     7921 tactccttgt atggcggacc agttggctcc tcctaccgat gggagctctc tgtttcctca
     7981 ttggtatctt ctataccttt ggacctattc cgctttcgag aatgccactg ggtgaagttt
     8041 tctctggagt aaccatggga tttggcatct tctttcttgc tgtatttatt cagcaaccag
     8101 atttgctact gaccagccaa gtgagcggtc agtggatgac tttgcaattt tcttggataa
     8161 aaataattga aattattttc atgtccattc ctctggtaac cttgattgct aatatcatgt
     8221 tggctaacaa cacctgtgat ttagaagaag atattcgcaa ccatcgttat actctggttt
     8281 actatattgg caagaaaaat gctcttaaat tgtattttgt cttggcaagt ttaccatggt
     8341 tgctgtggat aatctactgt ctgataggat tcttaccaat ttgggcactg gccggtttaa
     8401 tcggagtttg gcctgcttat aagagtctag aaacattttt gaaaaagcaa gtcaaacggg
     8461 aaacctttat cgaggcagta aagagttttg tactctttgc gctgctatat gtggtgatac
     8521 ttgctttagc ccttattttc taaaaaggaa agacacagtc gttcataagg ctgtgtcttc
     8581 tttgttatat cgtcagttag tttttctttt attacttcga cgagcgagga aacatcgttt
     8641 cctaatttcc aacttcaaac actccactgg agtatttgaa ctcgccttgc ctgattgaaa
     8701 cagtctgggg gactgtttca agtcaatgcc tagaaataag aaagcattga gttatgcctg
     8761 cggcttttga tgcgaacttt gttcgcttta tctccaacct ccaaagattt cccaaacctt
     8821 tggagctagt actaaaagta aactaaaaga ctatagtggt aaaatgagtt cttcttcatc
     8881 agtgctttct ccgccaacgg atttgatttc aatcaacagg ttatgtggca ggcagacgga
     8941 gagttgaccc ggctggctaa tccagctggt cataacggca atttggtctg ggctattgtc
     9001 ttccttgaca cgaatgcgtt cgccatcaac ttcaatgata ttgtattgcc cttcattggg
     9061 atagtaggtc ttttcttgat gaggtgtgtc ctttgataat tcaaattggt cgaccacctc
     9121 accattgatg cggacatagg caattgtttt tgcctcatta gtatctgtag tcagatgtgt
     9181 gtaggtaaaa attgctggaa gaaaggataa aactagggtg aatccgatta agatataatc
     9241 aaatagtttg agttgtttga gaatggattt gaatgtcata tacaatctat tttatagaaa
     9301 aaatcctact ttgtaaaatg ttggatgcag actacttttt caattgtcta aagaagagta
     9361 ggatggctaa cattccccaa aatccagcat gaagtggctg aatttttcca gcgaaaaata
     9421 caatggagaa agttagggca aaacctgcaa agagaaacca gagcagtggt ttatgataaa
     9481 gatagatagc tttttggaaa gcatttgctt ctgggccact agtctgccag tcactattca
     9541 agattggaag catagtgaac agtaagataa aaattggtgg attgtcccca gtcaggtagc
     9601 tatgtatata caggctacaa attgctgtat aggagagaac aatcaaagtg agtatgaatt
     9661 tcggtttgtc aacgttcatc tggcttctcc tttttgaaat aaatccgata aagaatagcg
     9721 gcagcaaatc cagcaggaag gatgaggata agtggtttga catgtcctaa ccaaatgccc
     9781 acggaatagg tcaaggtcat ggcaaacagg gcaaagatgg cttgaatgat aaagtagcgt
     9841 ttttcttctg ctgtttgata ggatttgatg atttggatgg tcggtagcat gagcaggaat
     9901 gaaccgttaa tcggctcatt gaccatgtaa gagtagatga agaaggcaga aaataaagca
     9961 taaaatagaa caaaaaatcg acaggtttgt ttgggtgtaa aaggtttatt catggggtca
    10021 gctccttatt ttttcttgac agaatctgct tccagttttt gcagataggc aatggcagac
    10081 agggctagac tggcaaagag gcccatcatg ccaccgaaga ggagggattt tacgtggaag
    10141 atggaagcca gtaggggaag ctggaggcta aagaggtctg ccagcccagc aaagaggcta
    10201 gatagcaggg caactcccag tcccaaaacc agtggattga gggtctgtcc ttgtttttcc
    10261 tcagggctca gttcgtccat gtcatattga ggcaggtcag catggagggt gcgcat
//
LOCUS       DASFSW010000076        10307 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.076, whole genome shotgun sequence.
ACCESSION   DASFSW010000076 DASFSW010000000
VERSION     DASFSW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.076"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            334..885
                     /locus_tag="U1099_001367"
     CDS             334..885
                     /locus_tag="U1099_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEM4867686.1"
                     /translation="MIVFLVICIFLVRIYFLKISIKNEKRILKNGGSEFGVQNTKLLT
                     VVHILFYLSCLVEAVVRHTKFDTLSSLGLIFLLFSMGMLFWVTRLLGDIWTVKLMLVK
                     DHRFVDHWLFRVVKHPNYFLNIIPELIGLALLCHATYSFLILFPVYMVILYRRIHEEN
                     NLLKTVIIPNGTVQSAAEITGNN"
     gene            complement(1094..2002)
                     /locus_tag="U1099_001368"
     CDS             complement(1094..2002)
                     /locus_tag="U1099_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEM4867687.1"
                     /translation="MKIDDLRKSSNIEDRRGQSSSNSSFSSGSSGGNVLLGPIFSRLG
                     WKGKLLVLVLLVTFGGMSNLGGLLSPSTGTNPYQSSQVTTTGTDVSDADAEFMSKVLG
                     STEDFWVQEFKANGLTYNAPTMVFYTNQTQTGCGVGSAQAGPFYCSADRKIYIDTSFY
                     QELSSKYKAAGDFAMAYVIAHEVGHHVQNELEVLGAYHQKRAQLSDKEGNALNVRLEL
                     QADYYAGAWAKYVDGQGILDAGDIDEAMNAAHAVGDDTLQQEAYGRTVPDSFTHGTSE
                     QRKKWFNLGYTYGDLAHANTFSVEKP"
     gene            2170..3513
                     /locus_tag="U1099_001369"
     CDS             2170..3513
                     /locus_tag="U1099_001369"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEM4867688.1"
                     /translation="MVEYLSVSHLTKYLKLKFDRDPYLEKVYLTGQVSNFRKRPSHQY
                     FSLKDDKAVIQATMWAGVYKNLGFELEEGMKINVIGRIQLYEPNGSYSIVIEKAEPDG
                     IGALAIKFEQLKQALTQEGLFKEEFKQALPRFTKKIGVITSPSGAVIQDIITTVSRRF
                     PGVEIVLYPTKVQGDGAAQEVVTNIQRANERDDLDVLIVGRGGGSIEDLWAFNEEIVV
                     RAIFESRIPIISSVGHETDTTLADFVADKRAATPTAAAELATPVTKADLLGYLNQQEN
                     RAYQAMTNRLRFLRGQLEKISQSVMFRQPERLYDGYLQKLDRLTNQLQTRMKELYSQQ
                     KQACLLLQRRLQGVHPGRKVESFQERIIQQERLLKSNMANIYDNKMAKADKLIEALTM
                     LDTSRIVARGYAMIRQDGRVIDSVENVQMGENLTIQMKDGQLDVEVKHVQTDENI"
     gene            3488..3703
                     /locus_tag="U1099_001370"
     CDS             3488..3703
                     /locus_tag="U1099_001370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEM4867689.1"
                     /translation="MSKQTKTFEENLAELEGIVTKLERGDVALEEALAEFQKGMVLSK
                     DLQKTLAEAEKTLVKVMQADGSEAEMD"
     gene            3703..4572
                     /locus_tag="U1099_001371"
     CDS             3703..4572
                     /locus_tag="U1099_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM4867690.1"
                     /translation="MVASKLQRLEEAMADFYQPQVAERLNQSVLYSLQAGGKRIRPLL
                     LLTILESFGMELKQAHYQVAACVELIHTGSLIHDDLPAMDNDDYRRGRLTNHKVYDEA
                     TAILAGDSLFLDPFGFLAQVDLPAETILALVRELSQASGTFGMVAGQVLDMAGEGQNL
                     TLEQLQAIHANKTGKLLTFPFVAAGLIAGQSDVVINHLRQAGQLIGLAFQVRDDILDV
                     TTTFEEIGKTPQKDVLAEKSTYPALMGLEASKVFLEDSLNQAINELDQVAKQVPFSTE
                     KIKEIIESLRING"
     gene            4565..5386
                     /locus_tag="U1099_001372"
     CDS             4565..5386
                     /locus_tag="U1099_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HEM4867691.1"
                     /translation="MAKERVDVLAFKQGLFETREQAKRGVMAGLVISVINGERYDKPG
                     EKIDEGIELKLKGEKLKYVSRGGLKLEKALQVFHLSVEGQTTIDIGASTGGFTDVMLQ
                     AGAKQVYAVDVGTNQLAWKLRQDERVISMEQYNFRYAELGDFELGQPSFASIDVSFIS
                     LSLILPALHRILAEGGQVVALVKPQFEAGREQIGKNGIVKDKSVHLKVLEEVTKMAVE
                     TGFSVKALSFSPVQGGHGNIEFLAHLEKSTQPNQIDKEVITTTVYQAHEEFKKDE"
     gene            5379..5804
                     /locus_tag="U1099_001373"
     CDS             5379..5804
                     /locus_tag="U1099_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM4867692.1"
                     /translation="MNKRERLEVIKDLVVRFPIDTQEEIVERLEAMGVNATQATVSRD
                     IKELGIIKVPSADKGYIYGLPKAGLVKVKSQNVFEVSVMDKMINLRLVPGSSAVVKRQ
                     ILEQFQEHVFSVIADDDSILLIVTNEGIISQIEQTVRGW"
     gene            5807..7468
                     /gene="recN"
                     /locus_tag="U1099_001374"
     CDS             5807..7468
                     /gene="recN"
                     /locus_tag="U1099_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEM4867693.1"
                     /translation="MLLEVSIKNFAIIEQVSLNFENGMTILSGETGAGKSIIIDAMNL
                     MLGARATTDVIRHGAAKAEIEGLFSFENSRALEQILLEQGIEVADELIIRREILQNGR
                     SVSRVNGQMVNLSVLKQIGQYLVDIHGQHDQEELMKSQHHIRLLDSFGEDEFWSLKDR
                     YQTTFDAYRSLRKRVLEKQKNEQEHKARIEMLEYQIAEIEAADLKSGEDIQLNQERDK
                     LLNHKQIADTLTNAYALLDNEDFSSLNNLRSAMSDLQSLEEFDPDYKQLSSSLTEAYY
                     VVEDITKRLSDVVDNLDFDGNRLMQLESRLDLLNTITKKYGGTVDDVLDYFSKISEEY
                     NLLTGNDLSGDDLEVQLKNLEKELVERAGQLSQSRHELAVVLEDIIRQELQDLYMEKA
                     RFQVRFTKGKFNREGNETVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFARKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIYKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     DEHSTVSRVRLLTKEERIEEIAKMLAGDKITDAARNQAKELVEKG"
     gene            7562..8395
                     /locus_tag="U1099_001375"
     CDS             7562..8395
                     /locus_tag="U1099_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM4867694.1"
                     /translation="MSKIKIVTDSSTTIEPSLVEELNITVVPLSVMVDGVVYSDNDLK
                     EGEFLELMRGSKNLPKTSQPPVGLFAETFADLAKDGSQIIAILLSHALSGTVEAARQG
                     ATLSGADVTILDSSFTDQAEKFQVVEAARMAQAGASKEEILAAIETVKEKTELYIGVS
                     TLENLVKGGRIGRVQGMLSSLLNIRVIMEMKDHQLTPIVKGRGNKTFKKWLEEFTATL
                     ADKKVAEIGISYSGTADFANEMKSQLQEYVSKEISVLETGSIIQTHTGEDAWAILIRY
                     E"
     gene            8388..9221
                     /locus_tag="U1099_001376"
     CDS             8388..9221
                     /locus_tag="U1099_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM4867695.1"
                     /translation="MNNRKLIQGFVFFFLAILGSIFLFHQLIPVASSRISRQELTVSE
                     KISFRYVALGDSLTEGVGDTTGQGGFVPLLAQSLTNDYGYEVDYKNFGVSGNTSNQIL
                     KRMKEDGELISYLKTADLLTLTVGGNDLRKAIIKNIANLKVSTFDKPAKDYGKRLDTI
                     IKTARKNNPNLPIYVVGIYNPLYLNFPELTEMQTIVDNWNTMTEQITEKYQHVYFVPI
                     NDLLYKGLEGEAGISQNGSQVTNNLLYEEDSFHPNNTGYEIMKKAILERMNETTESWE
                     P"
     gene            9193..9789
                     /locus_tag="U1099_001377"
     CDS             9193..9789
                     /locus_tag="U1099_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEM4867696.1"
                     /translation="MRPRKAGNLNIWKWLFLAQLALLIGCGIVLYTRIQTDREDLTKL
                     VQTSTEDTKVGTFSTNREQLNQTLTNYLKEYQTEEFSYQLFVTSQQVVFEGSYQIFGV
                     TIPLYIYFQPSKMEDGSILLRITEISVGSLSLPKAEVLAYLQKNYKLPAFVKIDSEQA
                     QVQVQLTELKNKFGLYGKANTIDLYNDQFIVDIYRKRQ"
     gene            9879..10154
                     /locus_tag="U1099_001378"
     CDS             9879..10154
                     /locus_tag="U1099_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEM4867697.1"
                     /translation="MANKQDLIAKVAEATSLTKKDSAAAVDAVFGAVADYLAAGEKVQ
                     LIGFGNFEVRERSARTGRNPQTGAEIKIAASKVPAFKAGKALKDAVK"
BASE COUNT         3225 a         1897 c         2307 g         2878 t
ORIGIN      
        1 ttgctgtact ctgcaggggt gacttgctcc gcaagtgcta tctccaacct tgcacagtca
       61 ctcgactgta caagctgtct gcagctattg aagcgaactt gcttcgccct atctccaacc
      121 tccaaaggtt ccccaaacct ttggagcgag tactaaaagt aaactaaaag actataaaag
      181 agacatgatt tcatccattt ttgaaaacag gtctctttta ttgtagtctc aaatggaaaa
      241 tagaaaaaaa acaggagagg aatagaatgg cgacagtcta actttgtata atggatgtgt
      301 ttgataaaat ctgttaaaat acaggaggga tttatgattg tttttttggt tatttgtatt
      361 tttttggtga gaatctattt tttgaagatt tcaattaaaa acgaaaaacg gattttgaaa
      421 aacggcggtt cagaatttgg tgttcaaaat actaaattat taacagttgt acatattcta
      481 ttttatttat catgtttggt tgaagcagta gtgcgacaca ccaagtttga tacgcttagt
      541 agcttaggtt tgatattctt gctgttttca atgggaatgt tattttgggt aacacgattg
      601 ttaggtgata tttggacagt taagctcatg ttggtaaaag atcatcgttt tgttgatcat
      661 tggttgtttc gtgttgtaaa gcatcctaac tatttcctaa atattatacc agaattaatc
      721 ggtttagctt tattgtgtca tgcaacatat agttttctca tcttattccc agtctacatg
      781 gttatcttat acagacgtat tcacgaggaa aataatttgt tgaaaacagt gattattcca
      841 aacggaactg tgcagtctgc agcagagata actggaaata attaaagttt gctaaataat
      901 aattgtgata ttgtttgaaa ctaggatacc gctctaatta gagttgatga gtaagtttac
      961 atgattcagt tttcatattt aaaagtggta ccgtatgtgt aaattggctt ttgtcagttg
     1021 tatattataa agaaactctt gcaaaagtgt atgttgcaag agtttcttta ttgctacgag
     1081 aggctatgga tgtttacggt ttctctacgg agaatgtatt ggcgtgagca aggtcaccat
     1141 aggtataacc gagattaaac cattttttcc tttgctcaga agtgccgtgg gtaaaactgt
     1201 ctggaacagt acggccgtag gcttcctgtt gaagagtatc gtcaccaact gcatgggcag
     1261 cattcatggc ttcgtcaata tcaccagcat ccaagatgcc ttgtccatca acatatttgg
     1321 cccaggctcc tgcataatag tcagcttgca attctaaacg aacatttagc gcattgcctt
     1381 ccttatcgga tagttgagcc cgtttttgat ggtaggcccc aagaacttct aattcattct
     1441 gaacatggtg accaacttca tgagcaatga cataggccat ggcaaagtca ccagcagcct
     1501 tatacttgct agataattct tgatagaaag aagtatcaat ataaatcttg cggtcagcag
     1561 agcaatagaa tggaccggct tgggcggaac caactccaca tccggtttgg gtctggttag
     1621 tatagaaaac catagtcggt gcattgtagg ttaaaccatt ggctttaaat tcttggaccc
     1681 aaaaatcttc tgtcgatcct aaaaccttac tcatgaattc agcatctgca tcagacacat
     1741 ctgtaccagt cgttgtgact tgactcgatt ggtaaggatt agtccctgtt gatggggaaa
     1801 gcaacccacc aaggtttgac atgcctccga aggttactaa aaggacaaga acaagtagtt
     1861 ttcctttcca accaagtcgt gaaaagattg gtcccaatag aacatttccg ccagatgaac
     1921 cagacgagaa agatgagtta gaactggatt gtcccctgcg gtcctcaatg ttagaacttt
     1981 ttcgcaaatc atcaattttc atataaatct ccttagaacc tgtcaacaag ttcttatttt
     2041 ctcacaaact attatatagc aaattgttga aaaaaaacat aggaaagcta tcggaaataa
     2101 taggcctagt tctgtataaa agtgcaaaat ttggtataat agtagggata caatatagaa
     2161 ggagtggaca tggttgaata tttatcggtt agtcatctaa ctaagtattt aaaattaaag
     2221 tttgaccgcg atccgtattt ggagaaggtg tatttgacag gtcaagtgtc caacttccgt
     2281 aaacgcccta gccatcaata tttttcgctc aaggatgaca aggcagtcat ccaagcgacc
     2341 atgtgggcag gcgtctataa gaatctgggc tttgagcttg aagagggaat gaaaatcaat
     2401 gtcattgggc gcattcagct ctatgaacca aatggttctt attctattgt cattgaaaaa
     2461 gctgagccag atggtattgg agccttggca atcaagtttg aacagctcaa acaagctctg
     2521 acacaggaag gcttatttaa agaagaattc aagcaggcct tgccacggtt taccaagaaa
     2581 atcggtgtca ttaccagtcc gagtggagct gttattcagg atattattac aaccgttagc
     2641 cgacgttttc caggcgtaga gattgttctc tatccgacca aggtgcaagg agatggtgct
     2701 gctcaggagg ttgttactaa tattcaaaga gccaatgaac gggatgactt ggatgttctg
     2761 attgttggtc gtggcggtgg ttctatcgaa gacctttggg ctttcaatga agaaattgtg
     2821 gttcgggcta tttttgaatc ccgtattcca atcatttcca gcgtaggtca tgaaacagat
     2881 acgaccttgg ctgattttgt agcagataaa cgagctgcta ccccaacagc tgccgcagaa
     2941 ttagcaacac ctgtgaccaa ggcggactta ctgggctacc tcaatcagca ggaaaataga
     3001 gcctatcagg ccatgaccaa tcgtttgaga ttcctaagag gtcagcttga gaaaattagc
     3061 cagtcagtca tgttccgcca gcctgaacgc ttgtatgatg gttatttgca aaaattagac
     3121 cgcttgacca atcaattgca gacacggatg aaagaactct acagccagca gaaacaggct
     3181 tgtctcctac tacagcgacg attacaaggt gtgcatccag gtcgtaaagt agaaagtttc
     3241 caagagagaa ttatccagca ggaacgactc ttgaagagca atatggctaa tatctatgac
     3301 aataagatgg ccaaggcgga caagctgatt gaggccctga ctatgctgga taccagtcgc
     3361 atcgtagccc gtggctacgc catgattcgt caagatggtc gggtgatcga tagtgtagaa
     3421 aatgtacaaa tgggtgaaaa cctaaccatc cagatgaagg atggtcaact tgatgtggag
     3481 gtaaaacatg tccaaacaga cgaaaacatt tgaagaaaac ttagctgaat tagaaggaat
     3541 cgtaaccaaa ttggagcgag gcgatgtggc tctggaagaa gcactggccg agtttcaaaa
     3601 aggaatggtt ctatcgaaag acctacaaaa gacactagca gaagctgaaa agaccttggt
     3661 caaggtcatg caggcggatg gcagtgaagc ggagatggat taatggttgc cagtaaattg
     3721 cagcgacttg aggaagccat ggcagacttt taccaacccc aggtagctga acgcctcaac
     3781 caatcggttc tttattcgct acaggctggt ggtaaacgaa tccgcccctt gcttttattg
     3841 accatcttag agtcctttgg gatggaatta aaacaagctc attaccaagt ggcagcatgt
     3901 gtcgagttga tccatacggg aagtttgatt cacgatgacc tgcctgctat ggataatgat
     3961 gattaccgtc gtggtcgctt gaccaaccac aaggtttacg atgaagcaac ggctatttta
     4021 gcaggggaca gtctctttct agatccattt ggtttcctag cccaagttga tttaccagct
     4081 gagaccatcc ttgctctcgt gagagagctt tcccaggcat ctgggacctt tggaatggtt
     4141 gctggtcaag tcttggatat ggctggtgaa gggcagaatt tgaccttaga acaattacaa
     4201 gccatccatg ccaataagac tggaaaatta ttgacatttc cctttgtagc agcaggtttg
     4261 attgcaggac aatcagatgt ggtaatcaac catttgcgac aagcgggtca attaattggt
     4321 ctagcctttc aagttcggga tgatattttg gatgtgacaa ccacttttga ggaaatcgga
     4381 aaaactcccc aaaaagatgt attggctgag aagtctacct accctgccct aatgggactg
     4441 gaagcctcaa aagtctttct agaagatagc ttgaatcaag caatcaacga gctagatcaa
     4501 gtggctaaac aagtcccatt ttcaacagaa aaaatcaaag aaatcataga aagtttacga
     4561 atcaatggct aaggaaagag tagatgtact agcttttaaa caggggcttt tcgagacgcg
     4621 ggagcaggcc aaacgcggtg ttatggctgg cttagttata tccgtcatta acggggaacg
     4681 ctacgataaa ccaggtgaga agattgacga gggaattgaa ctcaagctca agggggaaaa
     4741 actcaagtat gtcagccgtg gtggtctcaa attggagaaa gccttgcagg ttttccactt
     4801 atcagttgag ggtcaaacaa cgattgatat tggggcttca acaggcggat ttacggatgt
     4861 tatgttgcag gctggtgcca agcaggtcta tgctgtggat gtagggacca accaactcgc
     4921 ttggaaatta cgtcaggacg aacgtgtaat cagcatggaa cagtacaatt tccgctatgc
     4981 tgagttggga gatttcgaac ttggtcaacc aagttttgcg tccattgacg tcagctttat
     5041 ctcattgagt ctgattttgc cagcactcca tcgtatttta gcggagggtg gccaggttgt
     5101 tgcccttgtc aaaccccaat tcgaggctgg acgtgaacag attgggaaaa atggcattgt
     5161 taaggataaa tccgttcact tgaaagtctt ggaagaagtg acaaaaatgg ccgtggaaac
     5221 aggcttctca gtcaaagccc ttagcttttc ccctgttcaa ggcggtcatg gcaatatcga
     5281 atttttagcg cacttagaaa aatcaacaca accaaaccag attgataaag aggtcataac
     5341 aactacagtc tatcaagcac atgaggaatt taaaaaggat gaataaacga gaacgattag
     5401 aagtcattaa agatttggta gtccgatttc ctattgatac acaggaagaa attgttgaac
     5461 gtctagaagc aatgggtgtc aatgcgacac aagcaacggt ttcacgtgat atcaaggaac
     5521 ttggcattat taaggttcca tcagcagata agggatatat ttatggttta ccaaaggcag
     5581 gtcttgttaa ggtaaaatcg cagaatgttt ttgaagtctc agtgatggat aaaatgatta
     5641 atctccgtct ggtacctgga agctctgctg tggtcaaacg ccagattttg gaacaatttc
     5701 aagaacatgt tttttcagtg attgctgatg atgacagtat tctgctcatt gttacaaacg
     5761 aaggtattat ttcgcaaatc gaacagacgg ttagagggtg gtagccatgt tactagaagt
     5821 gtccattaaa aacttcgcca ttattgagca ggtatcactg aattttgaaa acgggatgac
     5881 catcctatct ggtgaaacag gtgcaggtaa atccattatc attgatgcca tgaacctgat
     5941 gctaggggcg cgtgcgacga cagatgttat cagacatggg gccgccaagg ctgagattga
     6001 agggctcttt tcctttgaaa atagcagagc tctcgagcaa attttgcttg aacaggggat
     6061 tgaagtagct gacgaactca ttatccgccg tgaaatcctg caaaatggcc gttcggtcag
     6121 ccgtgtcaat ggacaaatgg tcaatttatc tgtcttgaaa cagattgggc aatatttagt
     6181 agatattcat ggtcaacatg accaggaaga attgatgaaa tcccagcacc atatccgtct
     6241 tttggacagt ttcggagaag atgaattttg gagtcttaaa gaccgttatc agacaacctt
     6301 tgatgcctat cgtagtcttc gcaaacgagt tcttgaaaag caaaaaaatg agcaagaaca
     6361 caaggcgcgg attgagatgc tagaatacca aattgctgaa atcgaagcag cggatttgaa
     6421 gtctggggaa gatattcaac tcaatcagga acgcgataaa ctgctcaacc acaaacaaat
     6481 tgcagataca ctgaccaatg cctatgcact gttagacaat gaagattttt caagcttgaa
     6541 caacctacgc tcagctatga gtgacttgca aagtctggaa gaatttgatc cagactacaa
     6601 acagctctct tctagcctga cagaagctta ttatgtcgtt gaagatataa ccaagcgtct
     6661 cagcgatgtg gtcgataatc tagattttga cggcaatcgt ctcatgcaat tggaaagtcg
     6721 tctggatttg ctcaatacca ttacgaagaa atatggtgga actgtcgatg atgttttgga
     6781 ctattttagt aaaatcagcg aagaatacaa tctattgaca ggcaatgatt tatctggaga
     6841 tgatttggaa gttcagctta agaacttaga gaaagaattg gttgaacgag caggtcagct
     6901 cagccaatca cgccatgaat tggcagttgt tttagaagat attattcgcc aagaattgca
     6961 agacttgtat atggagaaag ctcgtttcca agttcgattt actaaaggaa aatttaatcg
     7021 agaaggaaat gaaacagtag aattttacat ttctaccaac ccaggtgaag acttcaaacc
     7081 actggttaag gtagcttctg gaggagaatt gtctcgcttg atgttggcca ttaagtcagc
     7141 ctttgcccgt aaagaaggta aaacttctat tgtctttgat gaggtggata caggggtgtc
     7201 tggacgtgtg gcccaggcaa ttgcccagaa aatttataaa atcggacaat atggtcaagt
     7261 attggcgatt tctcatctac cacaagttat tgctattgca gattatcagt tctttattga
     7321 gaaaatatcc gacgaacatt cgactgtttc tcgtgtccgt ctcttgacca aagaagagcg
     7381 tattgaggaa attgccaaga tgttagccgg tgacaaaatc acagatgcag cccgtaatca
     7441 agcaaaagaa ttagtagaaa aaggttaggc gactagcctt tttgcttatg tccaaagttt
     7501 attttttagt taaaatttgt tataatagat ggcaaaattg aagagaattg taggatgaaa
     7561 tatgtcaaag attaagattg ttacggattc aagtacgact attgaaccca gtttggtcga
     7621 agaattgaat ataacagttg ttccattatc tgtaatggtt gacggagtcg tatactctga
     7681 caacgattta aaagaaggag aattcttaga gctcatgcgt gggtcgaaaa acttgccaaa
     7741 aacaagtcag cccccagtag gactctttgc ggaaactttt gctgatttgg ctaaagatgg
     7801 cagtcagatt attgctattc tcttgtcgca tgccttgtca gggacagtag aagctgctag
     7861 acaaggagca accctatcag gtgctgatgt gacaatactc gattcaagct ttacagatca
     7921 ggctgaaaaa ttccaagttg tagaagcagc acgcatggca caagctggtg ccagcaaaga
     7981 agaaatcctt gcagctattg aaacagtcaa agaaaaaacg gaattataca tcggtgtatc
     8041 aaccctagaa aatctggtca agggtggtcg tattggacgt gttcaaggga tgctgagtag
     8101 cctactcaat atccgtgtca tcatggaaat gaaagaccat cagctgactc cgattgtcaa
     8161 aggacgaggt aataagacct ttaaaaaatg gctggaagag ttcacggcaa ctctagcaga
     8221 taaaaaagta gccgagattg gtatttccta ttcaggaacg gctgattttg caaatgaaat
     8281 gaagagtcaa ttacaagagt acgtctcaaa agaaatttct gttttagaga caggttccat
     8341 cattcaaact catactggtg aggatgcctg ggctattttg attcgttatg aataacagga
     8401 agttgattca aggctttgtt ttcttttttc tggcaattct agggtcgatt tttctatttc
     8461 atcaactcat tcctgttgcc tcatctcgca tctccagaca agagcttacc gtatcagaaa
     8521 aaatcagttt tcgatatgtg gcacttggtg attctttgac agaaggtgtt ggagatacaa
     8581 ctggacaggg gggctttgtt cccttgttgg ctcaatctct aactaacgat tatggttatg
     8641 aagtagatta taaaaatttc ggagtttctg ggaataccag taatcagatt cttaagcgaa
     8701 tgaaggaaga tggagagctg ataagttact taaaaacagc agatctgtta actctgacag
     8761 ttggtggcaa tgatttgcgc aaggctatta ttaaaaacat tgccaatctc aaagtttcaa
     8821 cttttgataa gcccgcaaaa gattacggaa aacgattgga tacaattatt aagacagcgc
     8881 gtaagaataa tccaaatctt cccatctatg ttgtcgggat ttacaatcca ctctacctca
     8941 attttcctga attgacagaa atgcagacaa ttgttgataa ttggaatacg atgacagaac
     9001 agataactga aaaatatcaa catgtctact ttgttccaat caatgatttg ctctataaag
     9061 gattggaagg ggaggctggt attagtcaaa atggtagcca agttaccaat aatttactgt
     9121 atgaggaaga cagtttccat cccaataata ctggatatga gattatgaaa aaagcgatat
     9181 tggagagaat gaatgagacc acggaaagct gggaacctta atatttggaa atggctattt
     9241 ttagcccagt tggctttatt gattggttgc ggaatagtcc tctatacacg gattcaaaca
     9301 gatagagagg atttaacaaa gctcgttcag actagtacag aagacacaaa ggttgggacc
     9361 ttctctacta atagggaaca gctcaaccaa accctcacta actatctaaa agagtatcag
     9421 acggaagagt tttcctacca attattcgta acgagtcagc aagttgtttt tgaaggcagc
     9481 tatcagattt ttggtgtgac tatcccactc tatatttatt ttcaacctag caaaatggaa
     9541 gatggcagta ttttgttacg gataacagaa atatcagttg gaagtttatc cttgccgaaa
     9601 gcagaggtat tagcttattt acaaaaaaat tataaactac ctgcctttgt aaaaatagac
     9661 tcagaacaag cgcaagtaca ggttcaactg acagaactga agaacaaatt tggtctgtat
     9721 ggaaaggcga atacgattga tttgtacaac gaccaattca ttgttgatat ctacagaaaa
     9781 agacagtaaa atagcattaa aaacttgacc aaacgcctaa aattcggtac tatatagaag
     9841 tgagtaataa ctcagaaaat tattggagga cttagcaaat ggctaataaa caagatttga
     9901 ttgcaaaagt tgcagaagca acttcattga ctaaaaaaga ctcagcagca gctgttgatg
     9961 cagtattcgg agcagtagca gactaccttg cagctggtga aaaagtacaa ttgatcggtt
    10021 tcggtaactt tgaagttcgt gagcgttcag cacgtacagg tcgcaaccct caaacaggtg
    10081 cagaaatcaa aatcgcagca tcaaaagttc cagcattcaa agctggtaaa gctcttaaag
    10141 acgctgttaa ataattcagc attaaaaagc ccgtcatatc aagctttcta gcttgtccga
    10201 cgggcttttt tcggctcttt gtcaactgta gtgggtagat aaaaagctaa cacctagaga
    10261 ggacgaactt cgttctctct ttctttatgt tcaaagcaat caaaatg
//
LOCUS       DASFSW010000077        10225 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.077, whole genome shotgun sequence.
ACCESSION   DASFSW010000077 DASFSW010000000
VERSION     DASFSW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.077"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..602)
                     /locus_tag="U1099_001379"
     CDS             complement(<1..602)
                     /locus_tag="U1099_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4867698.1"
                     /translation="MLKFIKHIASLFLFFVAYQIISGFLMVGLSLQAIPEFPAQLIEN
                     MIWICAIIGIVLGIAFTIVLWKFVYSRYTIDYSVSSSWFHKIQWPILLYIAFFIFQLL
                     VPISESQNQTLVIQFVSAYPLVSFLSVVIFAPILEELIFRGLLATYFFPKMVDMKAVG
                     IYLAVTGSLFSLVHMPTTILQFLIYFTMGLNLGWLYLIRR"
     gene            complement(612..929)
                     /locus_tag="U1099_001380"
     CDS             complement(612..929)
                     /locus_tag="U1099_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867699.1"
                     /translation="MKLFLWILVGFLFFNSLSDRKKVKQLQARVKKLQKTTKGENQMS
                     VLLKELVGSKAKIRFDEEISIACEYTILAVDEEWVKISRELAEGEIETKLVRVDNIVD
                     LSF"
     gene            complement(939..1628)
                     /locus_tag="U1099_001381"
     CDS             complement(939..1628)
                     /locus_tag="U1099_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4867700.1"
                     /translation="MNRLGNVKDWNVVKNWKFLLVGLAVVALNVLTQLAMFALPNFDG
                     NNLLIAAVLLLLAVVLGLILTGKLGLWKGEQKWGMVANVGFVVLAFIVMFGLKIIGGQ
                     LIMLEEGYGQTTANQEIINNSGLPTLVLFLFAVFFAPVLEELLFRGILMGRVFGKDSI
                     VGLLLSSFLFGLIHNPTNIGSWVVYGGMGLVLGLAYRISGKYTNALILHSLNNLLGFL
                     LMLLMQSLGLI"
     gene            complement(1654..1860)
                     /locus_tag="U1099_001382"
     CDS             complement(1654..1860)
                     /locus_tag="U1099_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867701.1"
                     /translation="MKENKKFISVLKIVYFVGLIGLGLYAVGYIFGEWLATGQIDLSN
                     LNILLPMVLGLPALLLFKKESDKN"
     gene            complement(1882..2640)
                     /locus_tag="U1099_001383"
     CDS             complement(1882..2640)
                     /locus_tag="U1099_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEM4867702.1"
                     /translation="MKQGKQISTKQRWMRYSAYMLISAILGGFIGFFGVLIFKFSLPS
                     YLNLDNFLIFLRIITFLIFAGTVYFGVKANQNYKLYHSISDEDEEHVDELYKKMYRNL
                     EYATISFNVAVSLTLLNLVLGFGVTFFEDGAIMYGSIFDVVFYVILLISQIFIMKLTQ
                     KIRDYKLSAFATVKEMKDFAEAMDEGEKQANYEMSFQIVFTLNQIVLPGLYLFLFVLS
                     MLLQERQITAFLVVAFLHIYINVMQVRMIRRYFK"
     gene            complement(2637..2852)
                     /locus_tag="U1099_001384"
     CDS             complement(2637..2852)
                     /locus_tag="U1099_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867703.1"
                     /translation="MEYVLKNRLKELRARDSLNQTELAKLAEVSRQTISLIERGEYTP
                     SVVIAMRIAQIFNENVENVFQLVEVGE"
     gene            complement(2865..3431)
                     /locus_tag="U1099_001385"
     CDS             complement(2865..3431)
                     /locus_tag="U1099_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HEM4867704.1"
                     /translation="MEAKKKLFEVNIMKNKKFVWGLNILAVALILPFAFVFGMIGSWL
                     IANGSQYWELSVSEVWLLFPLYILLIVVHEAIHGIFFKVFCPENPVKYGFKWKSGMAY
                     ATSPGSLYNRMQMLVISLAPFVVISLGLTMLAGFGMMNTTLYFMLATMHAASCAGDFY
                     YVYLLLVKFAKENIAVEDTETGLIIYQA"
     gene            complement(3582..3776)
                     /locus_tag="U1099_001386"
     CDS             complement(3582..3776)
                     /locus_tag="U1099_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867705.1"
                     /translation="MKQPFIITGTTIARAVEIGSILLLAYILLFDLLGWSDMDTALMT
                     TLIASITCIEASLQMEKKRR"
     gene            complement(3980..4432)
                     /locus_tag="U1099_001387"
     CDS             complement(3980..4432)
                     /locus_tag="U1099_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM4867706.1"
                     /translation="MEHIKDIWQEMYEKAKGHYAPHYVSPFIYSNHVVAAIEAEDGQI
                     YTGYCFEATAGVFHLCAERAAAFNMFQDSGQTTIKRIIAFRNQAPSGEGSGMPCGACR
                     EFLMQLSAENAQTEIMVDFDKRETITLGELMPYWWGEERMQAGTDHLE"
     gene            complement(4507..7125)
                     /gene="alaS"
                     /locus_tag="U1099_001388"
     CDS             complement(4507..7125)
                     /gene="alaS"
                     /locus_tag="U1099_001388"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEM4867707.1"
                     /translation="MKNLSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPDNPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFAFPKDKLYMTYYPDDMDSYNRWIALGVEPSHLIPLEDNFWEI
                     GAGPSGPDTEIFFDRGTDFDPENIGIRLLEEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAIFQGAKTNFETDLFLPIIREVEKISGKTYDQDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRASMHGQRLGITEPFLYKLVETV
                     GNIMESYYPEVLEKRAFIEKIVKSEEESFARTIHTGSQFAEQLMDKLAAEGKSEIDGR
                     DIFKLYDTYGFQVELTEELAEHRGMTLDIAGFEAAMKEQQDRARASVVKGGSMGMQNE
                     TLAAITEESTFVYGQTALEASLSVIVADNARTEAVSEGQALLVFDQTPFYAEMGGQVA
                     DHGFVKNATGDIVATVIDVQKAPNGQPLHTVELSASISVGQTYTLEIETKRRKGVEKN
                     HTATHLLHAALHNIIGEHATQAGSLNEQDFLRFDFTHFEAVTAEELRRIEEEVNEQIW
                     NAIPVVTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSVELCGGTHVGNTAEIGI
                     FKILKEEGIGSGTRRIIAVTGREAFLAYRDQEDALKEVAATIKSPQIKEVPNKVESLA
                     QQVRDLQKENAALKEKAAAAAAGDVFKDVKDANGIRYIASQVQVSDAGALRTFADNWK
                     QKDYSDVLVLVAAIGDKVNVLVASKSSDVHAGNLIKVLAPIVAGRGGGKPDMAMAGGS
                     DSSAIQTLLNSVAENL"
     gene            complement(7405..7890)
                     /locus_tag="U1099_001389"
     CDS             complement(7405..7890)
                     /locus_tag="U1099_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEM4867708.1"
                     /translation="MKQFQVKQRFTFGGEKFDIRDSFGNLAYQVEGSFLEIPKRFIVT
                     NSRGGEVCQITKKFLTFLPQFTIDMVEGHSFYLQKEFTFFKDRYTVENLGLILDGNIW
                     DLDFRLKRPDGMLVAEISKELFHLTSHYSVTVLEDSYADLVISLVVAIDYIEMMEDAS
                     N"
     gene            complement(8155..9156)
                     /gene="prsA"
                     /locus_tag="U1099_001390"
     CDS             complement(8155..9156)
                     /gene="prsA"
                     /locus_tag="U1099_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEM4867709.1"
                     /translation="MKQTKKILAGAVTLFAAVTLAACSNAADKDIITMKGNTITVSEF
                     YEKVKTNSQAQQVLLSMVISNVFENQYGDKVSAEEVNKEYDKKAEQLGASFNAALSSA
                     GLTEESYKEQIRTNKLVEYAVKQAAEKELTDENYKAAYDAYTPEVTARVIKLADEAKA
                     KEVLAAAQAEGADFAQLAKDNSTDTTTKDNGGEVKFDSTSTTVPAEVQKAVFALDAGQ
                     VGASVISSVDMKTYTTSYYVVKLDAKSEKSAKWEDYKDKLKEIILAQKQRDSSFVATV
                     LKEALQKANVKVKDSAFQNLLSQYVTTEESSSSSTKSSSSSTESSSSTTESSSSSGQ"
     gene            complement(9217..9936)
                     /locus_tag="U1099_001391"
     CDS             complement(9217..9936)
                     /locus_tag="U1099_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEM4867710.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDFMRTRQAQNTGFLKELEEFA
                     HQENIPVIPHETVAYFRLLMQTLQPKRILEVGTAIGFSALLMADNSPHSKITTIDRNE
                     EMIGFAKENFAKYDYRQQIELLEGEAMDILPTLPDNTYDFIFMDSAKSKYIVFLPEVL
                     KKVKVGGLIILDDIFQGGDVAKDIKDIRRGQRTIYKGLQRLFDATLDNPDVTASLVSM
                     SDGLLMLRKNVENVDLKHIEI"
     gene            complement(10011..>10225)
                     /locus_tag="U1099_001392"
     CDS             complement(10011..>10225)
                     /locus_tag="U1099_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867711.1"
                     /translation="LDSGGLSPIASISEWARVIDNKYLDENFCLGAVDMDTDSYYLFF
                     SKQATFIRLQELAGNLGYRIDYAKNM"
BASE COUNT         3158 a         2300 c         1776 g         2991 t
ORIGIN      
        1 tctcgtctaa ttaaatagag ccaaccaaga ttgagaccca tagtgaaata aattaaaaac
       61 tgcagaatgg ttgttggcat atggaccaaa ctaaacaaag aacccgttac agcaagatag
      121 attccaacag ccttcatatc taccatttta gggaagaaat aggtcgccaa caaacctcta
      181 aaaatcaatt cctctaaaat cggagcaaag attacgactg ataaaaatga aaccaagggg
      241 taggcagaca caaattggat gaccaaggtt tgattttgac tttcagatat aggtaccaaa
      301 agttggaaga tgaaaaatgc aatatagagc aaaataggcc attgaatctt atggaaccat
      361 gatgaagata cactatagtc aatggtatac cgagaataca caaatttcca taatacaatt
      421 gtaaatgcaa ttccgagaac aatgccaata atcgcacaaa tccaaatcat attctcaatc
      481 aattgagctg gaaattctgg aatagcttgt aatgacagcc ctaccatcaa aaacccagaa
      541 ataatttgat aagcaacaaa aaataaaaac aatgatgcaa tgtgttttat aaattttaac
      601 atactccctc cctaaaaact caaatcaaca atattatcaa ctcgtaccaa cttggtttca
      661 atctctcctt ccgctaattc acgagaaatt ttaacccatt cctcatcaac tgccaaaatg
      721 gtgtattcac aagcaataga aatttcctca tcaaagcgga ttttggcctt acttccgacc
      781 aattctttca ataaaactga catctgattt tctccttttg tagttttctg caactttttt
      841 accctagctt gcaactgctt cacttttttc ctatctgata gagaattgaa aaatagaaat
      901 cctactagaa tccataaaaa tagtttcatc gtatcctcct atatcaagcc cagactctgc
      961 atcagtagca tcaacaaaaa tccaagaaga ttattcagac tgtgtagaat aagtgcattg
     1021 gtatatttgc cagaaatacg ataggcgagt cccaggacca ggcccatacc tccgtagaca
     1081 acccaagatc cgatattggt tggattgtga ataagtccaa acaaaaaact tgatagcaat
     1141 aatcccacaa tcgaatcctt accaaatact ctccccataa gaatccctct gaaaagcagt
     1201 tcctccaaaa ctggagcaaa gaaaactgca aagagaaaca aaactaaggt tggtaagcct
     1261 gaattgttga tgatttcctg attggcagtc gtttgaccat atccctcttc caacataatc
     1321 aattgcccac caattatttt cagaccaaac ataacgataa aagcgaggac tacaaaccca
     1381 acattcgcaa ccatccccca tttctgctca cctttccata gacctaattt accagtcaag
     1441 ataagaccaa gcaccacggc taacaataaa agaactgcag caatcagtag attattaccg
     1501 tcaaaatttg gtagggcaaa catggccaac tgggtcaata cattcagtgc aacgactgcc
     1561 agtcccacta agagaaactt ccaattttta accacattcc aatctttgac atttccaagt
     1621 ctattcatag caatctcctt cacatacact aaatcaattc ttatcacttt cttttttgaa
     1681 caatagcaga gctggcaagc ccaaaaccat cggcaagagg atattcagat tagacaaatc
     1741 aatctgccca gtagccagcc attctccgaa aatatagcca actgcgtaca gaccgagacc
     1801 gattaacccc acgaagtaaa caatttttag aaccgatata aactttttat tctctttcat
     1861 aatcactacc aaaaccttcc cttatttaaa ataccgacgg atcatgcgaa cctgcatcac
     1921 attgatataa atgtgcaaaa acgccacaac tagaaatgcc gtaatttgtc gttcctgcaa
     1981 tagcatgcta agtacaaata ggaatagata caaacctggt aacacgattt gattcaaagt
     2041 gaagacgatt tggaaactca tttcgtagtt tgcttgtttc tcaccttcgt ccatcgcctc
     2101 agcaaagtcc ttcatttctt ttaccgtagc aaaagcggat aacttatagt cacgaatttt
     2161 ctgagttaat ttcatgataa aaatctgaga aatgagaaga atgacataga aaactacatc
     2221 aaaaattgac ccgtacatga tcgccccgtc ttcgaagaag gtaacaccaa atcctagaac
     2281 caaatttaac aatgtcaatg atacagcaac attgaatgaa atggtcgcgt actctaggtt
     2341 acgatacatt ttcttatata gttcatcaac atgttcctca tcctcatctg aaatggaatg
     2401 ataaagttta taattttgat tagctttaac accaaaatat acggttccag cgaaaattaa
     2461 aaatgtaata atcctcaaaa aaattagaaa attatctaaa ttgagataag aaggtaggct
     2521 gaatttaaag attaatactc caaagaaacc gataaagcca cccaaaattg ctgaaatcag
     2581 catatacgct gaatatctca tccatctttg tttcgttgaa atttgtttcc cctgtttcat
     2641 tctcctacct ccactaattg aaaaacattc tccacatttt cgttaaaaat ttgagcaatt
     2701 cgcatggcaa tgaccaccga gggcgtgtac tccccccgct ctatcagact aatggtctgc
     2761 cgtgatactt ctgctaactt tgccaactca gtctggttga ggctatctcg tgcccgcaac
     2821 tccttgagcc gattttttaa gacatattcc atgatgtttc ctttctaggc ttggtagata
     2881 atcaagcctg tttctgtgtc ttcgacagct atattttcct tggcaaactt gaccaataag
     2941 agatagacat aatagaaatc gcctgcacaa gaggccgcat gcatggttgc cagcatgaag
     3001 taaagagtgg tattcatcat cccaaatcct gctaacatgg tcaagcccaa actaatcaca
     3061 acaaagggag ccaagctgat tactagcatt tgcatccgat tgtagagcga acctggactg
     3121 gtcgcatagg ccatacccga tttccattta aacccgtatt tgacaggatt ttcaggacaa
     3181 aaaactttga agaagatgcc gtggatagcc tcatgcacaa ctattaaaag aatatagagt
     3241 gggaaaagca accatacctc cgacacgctc agttcccagt attgcgaacc gttagctatc
     3301 aaccaactgc ctatcattcc aaatacgaag gcaaatggaa gaatgagtgc cacagcaaga
     3361 atattcaatc cccacacaaa cttcttattt ttcatgatat tgacttcaaa taatttcttt
     3421 ttcgcttcca tgtttcttct ccttcatttg cctacaagct agactaaact atatagtcct
     3481 agagcaactc caatcagtaa gccgataaat aattgcccct gcaatcataa tcgctaccat
     3541 atctgctgaa atattacgtt tcatggtcta tctcctatta cttatctcct ttttttctcc
     3601 atttgtaaac ttgcttcaat acaagtaatt gaagcaatta gagtagtcat tagagctgta
     3661 tccatatcgc tccatcccag caaatcaaaa aggagaatgt aagccaataa tagaatggac
     3721 cctatttcga ctgcacgcgc aatggttgtt cctgtaatta taaaaggttg tttcatttgg
     3781 tgactcctta tctgttcatc gtatcagatg aaccttttcg attttcatct caatatcatt
     3841 gtaacacttc tttttctttt tgacaactat ctttgtcaaa aaaataataa tttttgtcaa
     3901 aaagtttatg aatgttgtca aattgactag aatgccatac aaaaaactcc tttatccaga
     3961 ggataaaaga gttcatttat tattccaagt gatctgtacc agcttgcatt ctttcttcac
     4021 cccaccagta aggcatgagt tcgcctaatg tgatggtttc tcgtttgtca aaatcaacca
     4081 tgatttctgt ctgtgcattc tcagccgaca gttgcatcaa aaattcacgg caagctccac
     4141 aaggcattcc tgaaccctct ccagagggag cttgatttcg aaaggcaata atgcgtttta
     4201 tcgtcgtctg cccactatcc tgaaacatat taaaggcagc ggctctctct gcacaaagat
     4261 gaaatacacc tgctgttgcc tcaaagcaat aaccggtgta aatttgtcca tcttctgcct
     4321 caattgcagc aacaacatgg tttgaataaa tgaaaggcga aacataatgt ggcgcataat
     4381 gaccttttgc tttctcatac atctcttgcc aaatatcttt gatgtgttcc atacgtactc
     4441 ctcattttag aatagaaaga tatccccttg ctagttgctt taacaaaggg atatacactg
     4501 actatattac aagttttcag ctacactatt caagagggtt tggatagcag aggaatcgct
     4561 accaccggcc atggccatgt ccggcttacc accaccacga cctgctacga ttggcgcaag
     4621 gactttgatg aggttacctg catgaacatc gcttgacttg cttgctacaa ggacattgac
     4681 cttgtcgccg atggccgcaa ccaacaccaa gacatcagag tagtcttttt gcttccagtt
     4741 gtcagcgaag gtacggaggg cacctgcgtc tgaaacttgg acttggctag caatgtagcg
     4801 aatgccgtta gcatctttga catctttgaa gacatcacca gcagcggctg ccgctgcttt
     4861 ttctttcaga gctgcatttt ctttttgcag gtcacgaact tgctgagcaa gactttcaac
     4921 cttgtttggc acttccttga tctgcggcga cttgatggtt gccgctactt ctttgagggc
     4981 atcttcttgg tcacgatagg caaggaaggc ttcacggcct gttaccgcaa tgatacggcg
     5041 agtacctgaa ccgatacctt cttctttcaa aatcttgaag ataccgattt cagcggtgtt
     5101 gccaacgtgg gtaccaccac agagttccac agagtagtca ccgatagtaa caacgcggac
     5161 ttctttaccg tatttttcac cgaagagggc catggcaccc atttccttgg ctgtgtcaat
     5221 atctgtctca actgtcacaa ctgggatagc attccaaatt tgctcattga cttcttcttc
     5281 aatacgacga agttcttccg cagtaactgc ctcaaagtgc gtaaagtcaa agcggaggaa
     5341 atcttgctcg ttcaaggaac cagcctgtgt tgcatgctca ccgataatat tgtgaagagc
     5401 tgcatggagc aagtgagttg ctgtgtggtt cttctcaaca cccttacggc gttttgtttc
     5461 gatttcaagt gtgtaggttt gaccaactga aatacttgcg gacagttcaa ctgtgtgcaa
     5521 tggctgacca ttcggtgctt tctgcacatc gatgacagtc gcaacaatgt ctccagtagc
     5581 atttttcaca aaaccatggt ctgcaacctg accacccatt tctgcataga atggtgtttg
     5641 gtcaaagaca aggagggctt gaccttctga gactgcttct gtacgcgcat tatcagccac
     5701 aatgacagat aagcttgctt caagagctgt ttgcccataa acaaaggtcg actcttctgt
     5761 gatagcagcc agggtttcat tttgcatccc cattgaacca cctttgacaa ctgaagcacg
     5821 ggcacggtct tgttgctctt tcatagccgc ttcaaaacca gcaatgtcaa gggtcatacc
     5881 acggtgctca gccaattctt ctgtcaattc tacttggaaa ccataggtat catagagttt
     5941 gaaaatgtca cgaccatcaa tctcagactt accttctgct gccaatttat ccatcaattg
     6001 ctcagcaaat tgtgaacctg tatggatagt acgagcaaat gactcttctt cactcttaac
     6061 aatcttctca atgaaggcac gtttttcaag tacttctgga tagtaacttt ccatgatatt
     6121 accaactgtc tccaccaatt tgtacaagaa tggctctgta atgcccaaac gttgaccatg
     6181 catagaagca cgacgaagga gacgacgaag gacataacca cgaccttcat ttccaggaag
     6241 ggcaccgtca ccaatagcaa aagaaagggc acggatgtgg tctgcaataa ccttgaagct
     6301 catgttgtcg ccatcttggt cataggtctt accagaaatc ttttctactt cacgaatgat
     6361 tggcaagaag agatccgttt caaagttggt cttagcccct tggaaaatag ctgctaaacg
     6421 ctccaaacct gcgcccgtat cgatgttttt gtttggcaat tccttgtact cagagcgagg
     6481 aacagctggg tctgcgttga actgggacaa tacgatattc caaatctcaa tgtaacggtc
     6541 attttcaata tcttcttcca agagacggat accaatgttt tcagggtcaa agtcagtacc
     6601 acggtcaaag aaaatctccg tatctggacc agaaggacct gcaccgattt cccagaagtt
     6661 atcttcaagc ggaatcaagt ggcttggctc aacaccaagg gcaatccaac gattgtatga
     6721 atccatatca tcaggatagt aagtcatgta gagcttgtct tttggaaaag caaaccattc
     6781 tgggcttgtc aagagctcaa agccccattc aatcgcttca tcacggaaat aatcaccaat
     6841 agagaagtta ccaagcattt caaacatggt gtggtggcgg gctgtcttac caacgttctc
     6901 gatgtcgttg gtacggattg atttttgggc atttgtaatc cgtgggttat ctggaatgac
     6961 agagccatca aagtatttct tgagggttgc cacaccagag ttgatccaca aaagagtggg
     7021 atcgtttaca gggacaagat ttgctgaagg ctcaacagag tgaccctttg atttccagaa
     7081 atccaaccac atttgacgaa tttgagcaga agataaattt ttcataaact aagataccaa
     7141 gcccgattag aaggcgactg aaaaatagga aactgacagg aaatcttgat ttcctaggca
     7201 gtttatcttt tttccgagcc ttccgagcgg gttcaattta acgaaacagt taaattaaac
     7261 atttcctttc atatagtaat aattgagggg aaagtccgta gcccgagtcc atttcagaat
     7321 aaactcgata ccctttcctt tcataagtga tagttaggcg ggttcaattt aacggaacag
     7381 ttaaactgaa tgattatttc acgattaatt cgatgcatct tccatcattt cgatgtagtc
     7441 aattgcgacc accaaggaaa tgaccaaatc tgcataggag tcctccaata cagtaacgga
     7501 ataatgagaa gttaaatgaa agagttcttt ggagatttca gctacgagca taccatctgg
     7561 ccttttcaag cgaaaatcca aatcccagat atttccgtct aggattaaac ctagattttc
     7621 cacagtataa cgatccttga aaaaagtaaa ttccttttgc aagtagaaag aatgtccctc
     7681 taccatatca atggtaaact ggggcaggaa ggtcaaaaac ttcttagtaa tctgacaaac
     7741 ttccccacct ctggaattgg ttacgataaa tcgttttgga atttcgagaa aagatccctc
     7801 cacttgataa gcaagattcc caaaactatc acgaatatca aatttttcac caccaaaggt
     7861 aaagcgctgt ttcacttgaa attgcttcat gataacctcc aaaaaaataa caagaaatca
     7921 aacgaagggc gaaaaaccgc ggtaccacct tcattcaatg acttgtcatt ctcatttaag
     7981 aaactgtatc cacaagcaaa aaacttttat atataagcca caaactgtga atacaggctc
     8041 tactattcaa ttgtaatatt aagtagcaaa atacttggct tagatggctc gcaacaaccg
     8101 ccaattttct gaactaaggt gtcaagtacc aattcttaat aagtaaatcg tcaattactg
     8161 acctgatgaa ctagaagatt ctgttgtaga agaagaactt tcagtcgacg aagagctgct
     8221 cttcgttgag gaagaactgc tttcttctgt tgtcacatac tgtgaaagaa gattttggaa
     8281 agcactgtct ttaaccttaa cgtttgcttt ttggagtgct tctttcagca cagtagcaac
     8341 aaatgatgaa tctctttgct tttgtgccaa aataatttct ttcagcttat ctttataatc
     8401 ttcccattta gcagattttt ctgatttagc atccaatttt acaacgtaat aactcgttgt
     8461 atacgtcttc atatctaccg aagaaatcac cgacgctccg acttgacctg catccaaggc
     8521 aaataccgct ttctgtacct ctgctggaac agttgttgaa gttgaatcga atttaacttc
     8581 gcctccattg tcttttgtag tagtatccgt ggaattgtct ttcgccaatt gagcaaaatc
     8641 tgcaccttca gcttgtgcag cagctaaaac ttcttttgct ttagcttcgt ctgccaactt
     8701 gatgacacgt gctgttactt caggtgtata ggcgtcataa gctgccttgt agttttcatc
     8761 tgtcaattct ttctcagcag cttgttttac agcatactca accaatttat tggtacgaat
     8821 ttgttcctta taggattctt ctgtcaatcc tgccgaagaa agtgccgcgt taaaagaagc
     8881 accaagttgt tctgccttct tatcatattc cttatttact tcttctgcag agactttgtc
     8941 accatactga ttttcaaata cattactgat aaccattgac agcaaaactt gttgagcttg
     9001 tgagtttgtt tttactttct cataaaactc agacacagta atcgtgttac ctttcatcgt
     9061 aatgatatct ttatcagctg cattggaaca agcagcgagt gttactgcag caaagagtgt
     9121 tactgcacct gcgagaattt ttttagtttg tttcattaaa gactctctcc ttttttcatt
     9181 aaccccttta ttataacaca aaagcttaaa aacttcttaa atttctatat gttttaagtc
     9241 aacattctct acatttttcc tcaacataag gagaccgtca ctcatggaaa caagactagc
     9301 agtcacatct ggattatcca aagtcgcatc gaataaacgt tgcaatccct tataaatcgt
     9361 tcgctgccct cttctgatat ccttgatgtc cttggcaaca tctcctcctt ggaagatgtc
     9421 atccaaaatg attaaaccac ctactttgac ctttttcaag acttctggta aaaatacaat
     9481 atattttgat ttagcagaat ccatgaaaat aaaatcatat gtgttatctg gtaaagtagg
     9541 aagaatatcc atagcttctc cttccagcaa ttcaatctgc tggcgataat catacttggc
     9601 aaagttttcc ttagcaaatc caatcatttc ttcatttcga tcaatcgtcg taatcttcga
     9661 gtgcgggcta ttgtcagcca tcagtaacgc agagaagcca atagcagttc caacttctaa
     9721 aatacgttta ggttggagtg tctgcatcag caagcggaaa taagctaccg tttcatgagg
     9781 gataactgga atgttttctt gatgagcaaa ctcttccagt tctttcaaaa accctgtatt
     9841 ttgcgcctga cgtgttcgca taaaatccac aatttcttcc ttgacaaccg gacggcgcat
     9901 attgtgattt gcgtttttac tatatgattc aaccatagaa tccattatat cataagccga
     9961 caagattttc ttcctcaaca cactaaaagg taggtttccc caccttttct ttacatattt
    10021 ttggcgtaat caattcgata ccctaaattt ccagctagtt cttgaagtcg aataaaagtc
    10081 gcctgcttac tgaaaaagag atagtaacta tccgtgtcca tatctactgc ccctagacaa
    10141 aagttttcat caagatattt gttatcaatg actcttgccc attctgaaat agatgcaatt
    10201 ggacttaatc cacctgaatc caatt
//
LOCUS       DASFSW010000078        10091 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.078, whole genome shotgun sequence.
ACCESSION   DASFSW010000078 DASFSW010000000
VERSION     DASFSW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.078"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            36..296
                     /locus_tag="U1099_001393"
     CDS             36..296
                     /locus_tag="U1099_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEM4867712.1"
                     /translation="MVFRANVGKVRTADGRYFDTGLPKGFSDLFGFRSDGQIFFIEVK
                     NEKGRVRPEQEKFIERMRKFGALAGVARSVEDALNIVEGGSK"
     gene            293..523
                     /locus_tag="U1099_001394"
     CDS             293..523
                     /locus_tag="U1099_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEM4867713.1"
                     /translation="MKPFDNVTKPKHYQGKHGMEALDVVKNFIWDLAGERAYYWGNVI
                     KYLLRFQQKNGVEDLKKARQHLDWLIEDMEEV"
     gene            520..822
                     /locus_tag="U1099_001395"
     CDS             520..822
                     /locus_tag="U1099_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEM4867714.1"
                     /translation="MKKYLSAIANFYILFSILATPLIVVYKVDELSQKLKQPIIIYQV
                     DNADTEMFGKVTAKDVVDGHYYVEVRPYGKFLVTKEQYHEIEIGQDMPEYLNGRRS"
     gene            819..2078
                     /locus_tag="U1099_001396"
     CDS             819..2078
                     /locus_tag="U1099_001396"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEM4867715.1"
                     /translation="MREAILEICGVKEVFEVPQTLMTKLFSHEQSDLLARIATIYGDR
                     QLDQFRDFFQEEGADRKKLKQDYTPDGVAELLARVSRGGKSLADICAGTGSLTIQYLN
                     YHPDVEFVRCEEFSAKVIPFLLINLAIRKIDAEVIHGDSLTRECFNVYSIQDGVISQI
                     DSPSDRKVEVVISNPPYSMAWTPISDERFDLFGLAPKTKADFAFLLHGFHQLEDGGSM
                     SLILPHGVLFRANSEGAIRQQLLEHGAIDTIIGLAPNLFLNTGIPVAILLLRKGRSQK
                     DVFFVDAKDEFTKGKAQNSLDVEHIKKITSVVSLRMTTERFSYIADWEKLVENGFNLN
                     IPRYVDTFIPEEVQPLGVILRELIEIDREIAETEREFARLFGQLVATDPTKQAELEAE
                     QELMREYVDKPRLSKLITEESEQLTLW"
     gene            2078..2503
                     /locus_tag="U1099_001397"
     CDS             2078..2503
                     /locus_tag="U1099_001397"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEM4867716.1"
                     /translation="MKLAKITELANIERSSGRVYPAGCTLIQISATRGQVLYHAEEAE
                     IPSHYAVFLPNDMVLPKYLYHAISCQAGHFIYTVQTGLNIQMDTLNEMKLKIHTDLEK
                     QAEIVKYLDVIEKMEAKEEATIDLLKQAKQTNLSKMFVG"
     gene            2514..3089
                     /locus_tag="U1099_001398"
     CDS             2514..3089
                     /locus_tag="U1099_001398"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEM4867717.1"
                     /translation="MNKQEAIEIIEQDKIQVGRLVETNSGAHSIQQKVKSVDYVPLEI
                     VVNTIDKIGLKKVVVPKYVAEWIEECKRSGWHLEKVLYRLDDDEKVGDWAYDENDDLI
                     PENVDMIARAWLDGYEIEQEKLYTVEIPNPNANKWNATVLKRRDNNDNAIELCVYVRP
                     DFSDSDFQLTESEIKQDFEWAWDAGFAKEVE"
     gene            3089..3301
                     /locus_tag="U1099_001399"
     CDS             3089..3301
                     /locus_tag="U1099_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867718.1"
                     /translation="MFELYFRINDNEPELQGTFDTAVEAEKYMQRLIDTKSRIKSWYI
                     RKAQRDGYWLYDYGAHNAFYMIKKAE"
     gene            3679..3888
                     /locus_tag="U1099_001400"
     CDS             3679..3888
                     /locus_tag="U1099_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867719.1"
                     /translation="MTNENLGVLLVDVPEPKRMKYSILVRKDGKHTIIDTDSELIVKT
                     YACRCTQEEAKKYPQFRWVALEELG"
     gene            3885..4088
                     /locus_tag="U1099_001401"
     CDS             3885..4088
                     /locus_tag="U1099_001401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867720.1"
                     /translation="MTIKELIKELQMYDEDKEVVLTIANVYPVLHEFVDLETGLVRLS
                     SDCQIGFEFNLLSDNRLEIEGVW"
     gene            4089..4358
                     /locus_tag="U1099_001402"
     CDS             4089..4358
                     /locus_tag="U1099_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867721.1"
                     /translation="MTTADKILFIMRHNGWTKDVCADEIGVHVTQLNRWLRGVIPSEK
                     NMNTIDSLYIQLVFKPKRPKYIPRKREKIVIEYPYYSHQRQLWEK"
     gene            4434..4856
                     /locus_tag="U1099_001403"
     CDS             4434..4856
                     /locus_tag="U1099_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEM4867722.1"
                     /translation="MTFFPEVDFEKTKANAKRKLKEYPRWRRVANDVDGQKVTAVYTF
                     EPRQANGNPSRPVERLAINRVDAEAELEAIEYAINNLLNPTHRRILYEKYLYAGKRYD
                     FEIYNDLYLSEASFYIELNDALLSFAEQYRSGSLLVQN"
     gene            5016..5089
                     /locus_tag="U1099_001404"
     tRNA            5016..5089
                     /locus_tag="U1099_001404"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5050..5052,aa:Arg,seq:tct)
     gene            5215..5527
                     /locus_tag="U1099_001405"
                     /pseudo
     CDS             5215..5527
                     /locus_tag="U1099_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5606..7027
                     /gene="terL"
                     /locus_tag="U1099_001406"
     CDS             5606..7027
                     /gene="terL"
                     /locus_tag="U1099_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEM4867723.1"
                     /translation="MMDRAAIRQQARFELARRDFFYYCHLMASDFYKPSRRYLVELCN
                     DLQGFLSDNEHNVLVINEPPRHGKSRTAGMFVQWLLGNDNDKKIMTGSYNETLSTVFS
                     KNVRNAIQETKADEDVVVFNDIFPDTHIKYGDAAMNLWSLEGGYNNYLATSPTGTATG
                     FGADIIIVDDLIKNAEEANNATVLEKHWEWFTNTMLSRLEEGGKIIIIMTRWHSQDLA
                     GKALIELPKSDYKVKHISMKAYDEATDTMLCDEVLSKQAYLQKTKTMGADIASANYQQ
                     EPIDIKGRLYSGFKTYVDKPTFKRISAYTDTADTGKDYLASYIYGVTMDNEAYILDVV
                     FTKEPMEVTEPLLAQKLAEWQVNTCDIESNNGGRGFARNVERLTQDSYQNRYTVFNWF
                     HQSQNKQARILTNTTWAIEHIYFPENWRHRWSELYQNLMSYQREGKNAHDDAADALTG
                     VVEAINDKIRTKAKVKRKSLYGL"
     gene            7040..8488
                     /locus_tag="U1099_001407"
     CDS             7040..8488
                     /locus_tag="U1099_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM4867724.1"
                     /translation="MEEVLVYSRSLYNEQNLDKDIIYKLILKHDKTANELKRLKDYYL
                     GKHAIEKHTRRSNLPNFKTVANHAKDIADTATGYFMGNAIRYPKTDDMDIEDLLKTFD
                     SADVDSTDLDNALNMAIYGRAYEYIYVKEDENELVTRSLEPENTFIVYDDSIEQKPLF
                     AVYYYQTKDDVTEETYYRAQVLTENLQYSMSLREQKKESEEGVPHNLGALPIIEYRNN
                     RYMVGDYEQQISLIDAYNSLMGNRVNDKEQAIDSILVLYGAALADTPEEAKEAMEILR
                     EEGLLELPKDASAEFLKNVLDEATVEVLRKALKEDIYTFSHVPNLSDENFAGNTSGVA
                     MEFKLLGLEMITKTKERYYIKSLHKRIQIFANYYNWSQIYENAKAIIPQFSRGLPKNL
                     LELSQIISNLKDKVSLRQLISLLPFVEDPDAEIEALEKEKEAAQEEPAFSQNLPYEES
                     VTDGQSEVLGEAESSANGSRNGQGRANRKTTR"
     gene            8439..9908
                     /locus_tag="U1099_001408"
     CDS             8439..9908
                     /locus_tag="U1099_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEM4867725.1"
                     /translation="MVQEMDKAEQTAKQLDEIHKLASRHITSKIDQIFESYRRDHGLT
                     EDEARRVLDNVKNLSDIRELKLALQNTTDSEEIRQLLILLDLAPYASRIERYEALQRE
                     VDNLPTRLYKAENEASRAFYDEFIPDAYYHSVFDLQQQSGVAFAFNRIDPEEIRAIQQ
                     TPWLGANYSERIWGNTQALASELQKQLAVSLLTGRSAHETAEVIEAQFGKGSQNARRL
                     IRTETSYFHAEMEAKAYEEADVEYYRFLATLDLRTSSICREHDGKIYKVSERITGKNY
                     PPMHPWCRSDTIASDDSEWLAKATRSARDPVTGKTIQVPANMTYKDWYEKYVKPKYKA
                     DNLDIWKIERANNQYEKYKSILGDKAPKSLEDYIDLKYNDKEGYGQLQDQARWIKAKF
                     PSEKSFNGHFEKHGHEFPSLTKEEYRKLASELIASPTDENILGYETEGGRRVRYDKAE
                     NIIVIGRRNKNNQARLNTMLKPDEGEKYYYENYKRDFPD"
     gene            9877..10077
                     /locus_tag="U1099_001409"
     CDS             9877..10077
                     /locus_tag="U1099_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867726.1"
                     /translation="MKTTNEIFLIDGEEYIRCPVCGRNVMLFDVCECNWENTGETNID
                     GGPNKMTLEEAKKAYAEGRPII"
BASE COUNT         3292 a         1761 c         2445 g         2593 t
ORIGIN      
        1 atgatattcg agttggtttg acggaagctg ggtgtctggt cttccgtgcc aacgttggta
       61 aagtccgtac ggcagatgga cggtatttcg atacaggtct gccaaaaggt tttagtgacc
      121 tgtttggatt tagatctgat ggacaaatat ttttcattga agttaaaaac gaaaagggtc
      181 gtgtgagacc agagcaggag aagtttatcg aacgaatgcg aaaatttggc gccttagccg
      241 gtgtagctag gagtgttgag gacgcgttga atattgtgga aggaggaagc aaatgaaacc
      301 attcgataac gtaacaaaac caaaacacta ccaaggtaag catggtatgg aagccttgga
      361 tgtggtcaag aattttatct gggatttagc aggcgagcgc gcctactact ggggcaatgt
      421 catcaaatat ctgttgcgat ttcagcagaa gaacggtgtc gaggacttga agaaagctag
      481 acaacatttg gattggttga ttgaggatat ggaggaagta tgaagaaata tctttcagcg
      541 attgcgaact tttatatttt attttcaatc ctggcgacac ctctaatagt cgtgtataag
      601 gttgatgagt tgagccagaa attaaaacaa cccatcatca tctaccaagt agacaacgct
      661 gatacagaga tgtttggcaa ggtcaccgct aaggatgtgg ttgatggtca ttactatgtc
      721 gaggttaggc cgtatgggaa attcctggtg accaaggagc aatatcacga gattgaaatc
      781 ggacaggata tgcctgagta tttgaatggg aggcggagtt gagagaagct attttagaaa
      841 tatgtggcgt caaagaggtt tttgaagttc cacagacact aatgacaaag ctgttcagcc
      901 atgagcagtc tgacttactc gctcgcattg ctaccatcta tggcgatagg caactggatc
      961 agttccgtga tttctttcag gaagagggag ctgaccgcaa gaagttgaaa caggactaca
     1021 cgcccgatgg tgtggcggaa ttactggcaa gggtgtccag aggtggcaag agtctagcag
     1081 acatctgtgc agggacgggg agtctaacca tccaatacct caactatcat ccagatgtgg
     1141 agtttgtgag gtgtgaggaa ttttctgcca aggttatccc gtttctgctg attaacttag
     1201 ccattcgcaa gatcgatgct gaagttatcc acggagatag tctgacacgg gaatgtttca
     1261 atgtctattc tatccaggat ggtgttatca gtcagataga tagtccaagt gacaggaaag
     1321 ttgaagtagt catctcaaat ccaccttact cgatggcatg gacgccaatc agtgatgagc
     1381 ggtttgacct ctttggacta gcacccaaaa ccaaggcaga ttttgccttt ctcttgcacg
     1441 gtttccatca actggaagac ggtggcagta tgtcactaat ccttccacat ggtgttctct
     1501 ttcgtgcaaa tagcgagggc gctattcgcc agcagttgct ggaacacggg gcaatcgata
     1561 ccattatcgg tctagcaccc aacctgtttc tgaatacagg tatcccagtt gcgattttgc
     1621 tcttgagaaa ggggcgaagc cagaaagatg tgttctttgt tgatgccaag gatgaattta
     1681 ccaagggcaa ggcacagaac agtttggatg tggagcatat caagaagatt acttcggtag
     1741 tgtccttgag aatgacaaca gagcggtttt cttacatagc tgactgggag aagcttgtcg
     1801 agaatggttt caacctgaat attccccgct atgtcgatac ctttatccct gaagaagtcc
     1861 aacccttagg ggttatcttg agggagttga tagagatcga cagggaaatt gctgaaacgg
     1921 agcgagagtt tgcgagattg ttcggtcaac tggttgcgac agatccaacc aagcaggcag
     1981 agctggaagc agaacaagag ttaatgcgtg agtatgtgga caagcctcgt ctttccaaac
     2041 ttatcacaga agaaagcgag caattaacat tatggtaatg aaattagcca aaattacaga
     2101 actagcaaac atagagcggt caagtggcag ggtctatcca gccggctgta ccttgataca
     2161 aattagcgcg acaagagggc aagttcttta ccatgctgaa gaagcggaaa ttccaagtca
     2221 ttacgctgta tttttgccga atgacatggt gttacctaag tatctctatc atgctatttc
     2281 ttgccaagca gggcatttca tctatacagt ccagacgggt ctgaatatcc agatggatac
     2341 cctgaacgag atgaaactga aaattcatac ggacttggag aagcaagcag aaatcgtgaa
     2401 gtatctggat gtgattgaga agatggaagc caaggaagag gcaaccatag atttattaaa
     2461 acaagcaaaa cagacaaatc taagtaagat gtttgtgggg taggagggaa cagatgaata
     2521 aacaggaagc gattgagatt attgagcaag ataaaataca agtaggtagg cttgtagaaa
     2581 caaacagtgg agcgcattca attcaacaaa aggtaaagtc agttgattac gtacctcttg
     2641 aaatcgtcgt caatacaatt gataaaattg gtttgaaaaa ggttgtggtg ccgaagtatg
     2701 tagcggagtg gattgaggaa tgtaaacgct ccggttggca tttggaaaaa gttctttata
     2761 gactggatga cgatgagaaa gtcggtgatt gggcatatga tgagaatgac gacttgattc
     2821 ctgagaatgt tgatatgata gcccgtgcat ggcttgacgg ctacgagatt gagcaggaaa
     2881 agttgtatac agtggagatt ccgaatccga atgcaaataa gtggaacgct acagtcctca
     2941 agcgcaggga taacaacgat aacgctattg aactatgtgt atatgtaaga ccagatttta
     3001 gtgactctga ttttcaactc accgaatccg aaatcaaaca ggattttgaa tgggcttggg
     3061 atgcgggatt tgcgaaagag gtggagtaat gtttgagtta tatttccgaa taaatgacaa
     3121 cgagccagaa ctacaaggca catttgacac ggcagtagaa gctgaaaagt acatgcaacg
     3181 tttgattgat accaaatcaa gaatcaaaag ctggtatatc cgtaaagcgc aacgcgatgg
     3241 ctattggttg tatgattatg gtgcgcataa tgcgttttat atgattaaga aggcggagtg
     3301 atgaggtctg agaaacgtag tttagctatc aacgaattaa aagaagcgct taaagataaa
     3361 ttagtgaaaa agatttgcgg tggtagagtt tgggtagtga gcgctgagaa acaggaggtg
     3421 ttaaatgacg accaacgttg tccaattcat accgaaacat gatatatgcc acgagtgcta
     3481 caagagaaaa gcaacaaagc tgtgtgattt tataattggt cagacaggaa tagcattcta
     3541 tcgaagtttc agtttattta aaaatcagca acaaaggttt cttacttgtg acaagttgct
     3601 ctgcaacaga tgttccaaaa gatttcacgg tatggattta tgcaaaaatc actttaaaaa
     3661 aattacaaga ggcaaccaat gactaacgaa aatctaggtg tgctactggt cgatgtgcca
     3721 gagccgaagc ggatgaagta ttctatcctt gtccgaaaag atggaaaaca tacgattatt
     3781 gatacggatt cggaattaat cgtaaagacc tacgcatgtc gctgcaccca agaagaagct
     3841 aaaaaatacc cacaattcag atgggtagcg ttggaggagt tgggatgacc attaaagaac
     3901 taatcaaaga attgcagatg tatgacgagg acaaagaggt tgttttaacg atagccaacg
     3961 tttatccagt tttgcatgaa ttcgtagatt tggaaacggg gttggttcgt ctttcgtcgg
     4021 attgtcaaat cggctttgaa ttcaatcttt tatcagacaa tcgtctggaa attgaggggg
     4081 tgtggtagat gaccacagca gataaaattt tattcataat gcgacataac ggttggacaa
     4141 aagacgtgtg cgcagatgaa ataggcgtac atgtaacaca actaaataga tggctaagag
     4201 gggtaatacc cagtgaaaaa aacatgaata ccatcgacag tttgtatatt cagcttgtgt
     4261 ttaaacctaa aagacctaaa tacataccga ggaagaggga gaagattgtg attgaatatc
     4321 cgtattacag ccatcagaga cagctgtggg aaaaataaaa aaagccagca ctacttgtac
     4381 cgactctgtg aataaaactc taaaactatt atatcacaga aaggtatgag cagatgactt
     4441 tttttccaga agttgatttc gaaaaaacga aagctaatgc aaaacgaaaa ctgaaagagt
     4501 accctcgctg gcgtagagta gcgaacgatg tagatggaca gaaagttact gcagtgtaca
     4561 ctttcgaacc gagacaagct aatggcaatc cgagcaggcc cgtggaacgc ttggcaatca
     4621 atcgagtaga tgctgaggca gagcttgaag ctatcgagta tgcgattaat aatctactca
     4681 atcctacgca tagacgtata ctttacgaga agtatcttta cgcaggcaag cgatatgatt
     4741 ttgaaatcta caacgacttg tacttatcag aggctagttt ctatatcgaa ctcaacgatg
     4801 ccttgctatc gtttgctgaa caatatagaa gcggaagttt gttagttcaa aattagagtt
     4861 ttgaccaaga aatcaaaagt ttttgtatag attattcgtt tttgtcgatg ttaaaatagt
     4921 attgtcaaga taccgagaag agataaacgt taacatttca gtcgttgtca attgacagcc
     4981 aatctcctta tacaatcgaa ctcggtatct aaattgggaa catagctcag ctggatagag
     5041 catacgagtt ctaatcgtac ggtcacaggt tcgagccctg ctgttcccgt ttaagagtaa
     5101 gtgaatcctg cggggacctt gactcggagg tctggtcaat cgcatatcgg accaagacct
     5161 aatatgcatt agtcacacaa cgaagtgtgg cttttttaat tttgattgga ggtgatgata
     5221 ttggcgaaaa caaagcgtgg tagaccaacc aagatgacgc aaggaacact acggaaatta
     5281 gaagagttgt ttgtgagagg attgagcgat gaagaagctt gtcttctagc tgatatagga
     5341 accacaactc tttatgatta ctgtaaggaa aatcctgaat tttcggagcg aaaagagttg
     5401 cttaagcaac gtgtgaaaac acgggcaaaa cttaatatat cgaaagcgat tgaagatggg
     5461 gatatagact tgtcaaaatg gtacttagaa cgtcgtgaca acgattttaa aacaaaacaa
     5521 gcagtgacac acgatggtga agtcaatatc aaccaaacca acccatttgc cgacttgaca
     5581 acggatgagt taaggaagtt gattgatgat ggatagagcg gcaatcaggc aacaagcacg
     5641 ttttgagtta gctcgtcgcg atttctttta ctattgtcat ttgatggcaa gcgacttcta
     5701 caaaccatct cgtagatact tagtcgagct ttgtaatgac ttacaaggct ttttaagtga
     5761 caacgagcat aatgtgttgg ttatcaatga accacccaga cacggaaaat caaggacggc
     5821 aggcatgttc gttcagtggt tgcttggaaa tgacaacgat aaaaagataa tgactggttc
     5881 gtacaacgaa acgttatcaa ctgtattttc gaaaaatgtc agaaatgcta ttcaggaaac
     5941 gaaggcagat gaagacgttg ttgtgtttaa cgacattttt ccagatacgc atataaaata
     6001 tggcgatgca gctatgaatt tgtggagttt ggaaggcggc tataataact acttggctac
     6061 aagtccaaca ggtacagcga ctggtttcgg tgctgacatt attatcgttg acgatcttat
     6121 caagaatgcg gaagaagcta acaacgcaac cgttttagaa aaacattggg agtggttcac
     6181 gaacacgatg ctttcacgtc ttgaagaggg cggaaaaatc attattatca tgacacgttg
     6241 gcattcgcaa gatttggcag gtaaggcgct gattgaactt cctaagtctg attacaaagt
     6301 caagcacatt agcatgaaag cttacgacga agctacggat acgatgcttt gtgacgaagt
     6361 attgagtaaa caagcttatc ttcaaaagac taagactatg ggagctgata ttgcgtctgc
     6421 gaactaccag caagaaccta ttgacatcaa gggcaggttg tacagtggat ttaaaaccta
     6481 tgttgacaag ccgacattta agcgtattag tgcttacact gatacggcag atacaggcaa
     6541 ggactatcta gctagttata tctacggcgt cactatggac aatgaagcgt atatcttgga
     6601 cgttgttttc acaaaggaac cgatggaagt aacagagcct ttgctagctc aaaaactagc
     6661 agagtggcaa gtcaatacat gtgacatcga aagtaataat ggcggtcgcg gattcgctag
     6721 aaacgttgaa cgtttgacgc aagatagcta ccaaaaccga tatacagttt ttaattggtt
     6781 tcatcagtct caaaataaac aagcaagaat tttgaccaat acgacctggg caatagagca
     6841 tatctatttc cctgaaaatt ggagacatcg ttggtcagaa ttatatcaaa atcttatgag
     6901 ctatcaaaga gaaggtaaga atgctcatga tgatgctgca gatgcactga caggcgtagt
     6961 cgaagcaatt aatgacaaaa ttagaactaa agccaaagtc aaacgcaaat cgctttacgg
     7021 cttgtagaaa ggagcaaaaa tggaagaagt attggtctat agtcgctcgt tgtataacga
     7081 gcagaattta gataaggata tcatttacaa attgattttg aagcatgaca agactgctaa
     7141 cgagttaaaa aggttaaaag attactacct tggaaaacat gctattgaga agcacacacg
     7201 cagaagtaat ctgccaaatt ttaagacggt tgctaatcat gctaaggata ttgcggatac
     7261 cgccacaggt tactttatgg gcaatgctat ccgctatcct aagaccgacg atatggacat
     7321 cgaggatttg ctaaaaactt tcgatagtgc agatgttgat tcgacagact tagacaacgc
     7381 tttgaacatg gcaatctacg gcagggctta tgagtacatc tatgtcaaag aggatgaaaa
     7441 tgagctggta actcgcagtc tagaaccaga gaacactttt attgtttatg atgattcgat
     7501 tgaacagaaa cctttgtttg cggtctatta ttatcaaacg aaggacgatg tgacggaaga
     7561 gacgtattat cgagctcagg tattgacaga gaacctgcaa tatagcatgt ctttacgtga
     7621 gcaaaagaaa gagtcagaag agggtgttcc gcataatctc ggagccctgc ctattattga
     7681 atatcgaaac aatcgctata tggtcggaga ttatgagcaa cagattagct tgatagatgc
     7741 gtataactca ctgatgggca accgtgtgaa tgacaaggaa caagctattg attctatttt
     7801 ggtcttatat ggcgcagcgc tggcggatac accggaagaa gcaaaagaag cgatggagat
     7861 actgcgagaa gaaggattgt tagaactccc taaagacgca agtgctgagt tcttgaagaa
     7921 tgtcttggat gaggctacgg tcgaagtact tcgtaaggcg ctgaaagaag atatttacac
     7981 ttttagccac gtccccaatc tctcagatga gaatttcgca gggaatacat caggggtagc
     8041 tatggaattt aagcttttgg gccttgaaat gattaccaag accaaagagc gatactatat
     8101 caaatcttta cacaaacgca tacagatttt tgcgaattat tacaactggt ctcagattta
     8161 cgaaaatgct aaggcgatta ttccgcagtt tagccgtggt ttgccaaaga atttgttgga
     8221 actttcccaa attatcagca atctcaaaga caaggttagt ctacgccaac ttatttcgct
     8281 cttgccgttt gtggaagacc cagatgcaga gattgaggcg cttgaaaaag aaaaagaggc
     8341 tgcgcaagaa gaacctgcat ttagccagaa tttgccttat gaagagagcg tgacagatgg
     8401 acaatcagaa gtattgggag aagcggaaag ctcagcgaat ggttcaagaa atggacaagg
     8461 cagagcaaac cgcaaaacaa ctcgatgaaa ttcacaagct agcaagtagg catatcactt
     8521 ctaagataga ccagattttt gagagttatc gcagagacca cggactgacg gaagatgaag
     8581 caagaagagt gttagataat gtcaagaatc tatcggatat tcgggagtta aaattagctt
     8641 tacagaatac aacggacagt gaagagatac ggcagttgct tatcttgtta gatttggctc
     8701 cctacgcttc cagaattgag cgatacgagg ctttgcaacg tgaggtggat aatttaccca
     8761 cccgattgta taaagccgaa aatgaggctt ctagagcctt ctatgatgag tttattccag
     8821 atgcttatta tcactctgtt tttgatttgc agcagcagtc tggtgtggca tttgctttta
     8881 acaggattga cccagaggaa atcagagcta tccagcaaac gccatggctg ggagcgaatt
     8941 actctgaaag gatttggggt aatactcaag ccttagcaag tgaattacaa aagcaattgg
     9001 cagtcagtct gttaacgggt cggtcagctc acgagaccgc agaagtcatt gaggctcaat
     9061 tcggaaaagg gagtcaaaat gctcgacggt tgattcgaac agaaacgagt tatttccatg
     9121 cagaaatgga agcgaaggct tatgaagaag cagatgtgga atattatcgt tttctcgcaa
     9181 cattagattt gagaacgtca agtatctgtc gagagcatga tggtaaaatc tacaaagtta
     9241 gtgaacggat aactggcaag aactatcctc ctatgcatcc ttggtgtcgg tcggatacaa
     9301 tagcatcaga cgattcagaa tggttagcca aagcaaccag aagcgccaga gacccagtga
     9361 caggcaaaac tattcaagtc cctgccaata tgacgtataa agattggtat gagaagtatg
     9421 tcaaaccaaa atacaaggcg gataacttgg acatttggaa gattgaacgt gccaataacc
     9481 agtacgagaa atataagtca attcttggag ataaagcccc taaatcgctt gaagactata
     9541 ttgatttgaa gtataatgat aaagagggat atggacagtt acaggaccaa gctagatgga
     9601 taaaagcgaa attcccgtct gagaagtctt ttaatggtca ttttgagaaa cacggtcatg
     9661 agtttccaag tttgactaaa gaagagtatc gaaaacttgc atcggaactg attgctagtc
     9721 ctaccgatga aaatattttg ggttatgaga ccgagggtgg acgtagagtt cgctatgata
     9781 aggctgagaa cattatcgta ataggtcggc gaaacaaaaa taatcaggcg cgattgaaca
     9841 caatgttaaa accagatgaa ggtgagaaat actattatga aaactacaaa cgagattttc
     9901 ctgattgatg gagaagagta cattcgctgc cctgtttgtg gacggaatgt catgttattt
     9961 gatgtgtgcg aatgtaattg ggaaaacact ggtgaaacaa atattgacgg cggtccaaat
    10021 aagatgacgt tagaagaagc aaaaaaagca tatgcagaag gcagaccaat tatttaaata
    10081 agcactcaag t
//
LOCUS       DASFSW010000079         9772 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.079, whole genome shotgun sequence.
ACCESSION   DASFSW010000079 DASFSW010000000
VERSION     DASFSW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.079"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..508
                     /locus_tag="U1099_001410"
     CDS             <1..508
                     /locus_tag="U1099_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEM4867727.1"
                     /translation="TTIAGAVLSFILGVTCFIFMIGLSSTNFDNWDTGSDGYIDETVE
                     YTEYQVGDSVDFSDGLHVTVTSMGKDDSIELVDSYYSTAYVVEMEVENSTAKELYFDE
                     YYFSLMDPVSQIPFTLDLRTYDVNLVEKLKPGEKVQVKLIYGIDNETNFGFTYEDAMW
                     TELVAEGI"
     gene            complement(743..2089)
                     /gene="gdhA"
                     /locus_tag="U1099_001411"
     CDS             complement(743..2089)
                     /gene="gdhA"
                     /locus_tag="U1099_001411"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEM4867728.1"
                     /translation="MSNAKAYIQASFEAVKARNPHETEFLQAVEELFSTLEPVFEAHP
                     EYIEENILARIVEPERIISFRVPWTDKYGNVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQSILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEIMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYMYGQYKRLRQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     FTDNMLAANGKSFKDQTVLISGSGNVAQYAVQKATELGAKVISVSDSNGYIIDETGID
                     FDLLVDIKEKRRARLTEYATEKATAKYFEGSVWNYDGKADIALPCATQNEINGEQAAA
                     LVKNGVYCVAEGANMPSDLDAIKVYKENGVLYGLAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMANIFNTAKETAEKYDLGTDYLAGANIAAFEQIADSMIAQGLV"
     gene            2312..3250
                     /locus_tag="U1099_001412"
     CDS             2312..3250
                     /locus_tag="U1099_001412"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEM4867729.1"
                     /translation="MVSTATTLGGFTFDNCLMNAAGVWCMTKEELDAVKNSKAGTFVT
                     KTATLDYRAGNPEPRYQNVPLGSINSMGLPNQGLAYYLDYLLELQETESERTFVLSVV
                     GMSPDETHEILRTVEASDFKGLTELNLSCPNVPGKPQIAYDFETTETILQEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNQFPLKFVNCVNSIGNGLYIKDESVVIKPKNGFGGI
                     GGEYIKPTALANVHAFYQRLKPEIQIVGTGGVLTGRDAFEHILCGASVVQVGTTLQKE
                     GVAAFGRITAELQAIMAEKGYETIEDFRGKLQHLDD"
     gene            3583..4815
                     /locus_tag="U1099_001413"
     CDS             3583..4815
                     /locus_tag="U1099_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEM4867730.1"
                     /translation="MERRANYIRRKRYASYRTKMHLFFICGLATIILAIIIVSEHLVP
                     RLNQASESMHSDNSVRIMAHGDLLYHLPILRGAEQPDGSYDFSENFTYVKPWIEQADF
                     AIADFEGTISPDIELAGYPLFNAPAIVANNIHDAGYDLVDLAHNHILDSHLSGLISTV
                     NTFRDAGVDTVGVYAEGNRATAPLYIREVNGIRIAVLAYAYGFNGMEALLSQEEYDAY
                     LSDFDKEKMRAEIERAEKEADITIVMPQTGVEYQLEPTEEQTSLYHQMIDWGADIVLG
                     GHPHVVEPTEILEKDGQRKLIIYSMGNFLSNQRIESMEDTPNAQWTERGVLMDLTIQK
                     ENGQTSIQTAQAHPTWVNRLSKGTYSTDGYEQFSYQTYILEDWVAGGRYYGQLDLDTQ
                     ARVDTVYQEMKDFVNLVW"
     gene            4827..5654
                     /locus_tag="U1099_001414"
     CDS             4827..5654
                     /locus_tag="U1099_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4867731.1"
                     /translation="MIVRLIATDMDGTFLDGQGNFDRERFSRVLDQLDQKKIPFVIAS
                     GNGIGRLLQLCQGFEDRLIFVADNGAHVYQNGKTVIRRAIQQVEVEAILHFFKGRWAD
                     VCLMLSNDGNIYMQAGAGMPFAGTDLPIESAQMVAFQNRVTYLDDLSAYPNFEPIYKV
                     GLWVPEARVESITEEFNQAFQGRLTAVTSGYGSIDILPEGIHKAWGLEQVLKDLVIQP
                     DQVMAFGDSDNDIELLSYVGYSYAMENATDKVKAVAKYMAPSHLEAGVLQVIEEYIL"
     gene            complement(5704..6486)
                     /locus_tag="U1099_001415"
     CDS             complement(5704..6486)
                     /locus_tag="U1099_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEM4867732.1"
                     /translation="MKKYISLYLYNIKVYMMAQMSFRVDFFIGLFSSLIEQIVYLIFL
                     NILFGNIKEIAGFNYGQMLFIYGIATVGRSIHLIFFDNLWMFGSRYIRQGEFERLLLM
                     PVNPLFQLICEQIQPQGIGTTLIGSLALVQASKELGMEWSLGKLALLIFIAICIGLLY
                     AAIQLGPTALAFWIVESFPLTMGIFALNQMAQYPLNIYPKIIQILLIFVFPYAFTAYF
                     PALYFLDLSMWGLALPLVVVVLFSINYNLFRYGMTKFTSVGN"
     gene            complement(6488..7267)
                     /locus_tag="U1099_001416"
     CDS             complement(6488..7267)
                     /locus_tag="U1099_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEM4867733.1"
                     /translation="MRKYLYMTRLTMMNAMQYKAFFLATFVSLAVKILVALYVWKTIF
                     FTQSEVNGFTLQTFTTYIIFANLLASLNSFSLGEDLSYSILKGSIAGEFLRPYSFILA
                     LFFKDLGNKLLELIKFAIVFIGILLVHQDFYLPDGKTILLFLVSSILGMFIVQLLDMA
                     FGFLAFFTVNAWGVMLLRMGLFNLASGALLPLSFYPQAVENFLKLLPYNYAVNVPVSI
                     LLGQESDLTALGLQVIWIPILACFIALLWSQAKRRIVIFGG"
     gene            complement(7260..8244)
                     /locus_tag="U1099_001417"
                     /pseudo
     CDS             complement(7260..8244)
                     /locus_tag="U1099_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8329..8862)
                     /locus_tag="U1099_001418"
     CDS             complement(8329..8862)
                     /locus_tag="U1099_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEM4867734.1"
                     /translation="MGTDSRKERTRQAILEAMVTCLESQGFNDITTTHLAQTAGISRS
                     SFYTHYKDKYELIDSYQQGLFHKLEAIFDRYDGNRQSSFLEIFELLYREKLLSALLTH
                     NGTQEIQNFLINKVRLLVANDLAERLGKEDLSPLEKEYSSIYFSHAFFGIMQVWIKNG
                     KQESPQYMTDFLIKMLP"
     gene            8988..>9772
                     /locus_tag="U1099_001419"
     CDS             8988..>9772
                     /locus_tag="U1099_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEM4867735.1"
                     /translation="MLRKVKNIFKKPMTLVTIIGIACVPALYNISFLTSMWDPYGRLD
                     QLPVAVINQDQSASFQDKTLTIGDDMVDNMKESKSLDFHFVSEKDAEKGLEEGDYYMV
                     ITLPEDLSEKATSLLTNQPEPITISYQTSKGHSFVASKMGESAMEKLKTSVSETITKT
                     YTTAVFDSMREIQTGMVEAADGSQQLTDGASQLESGSQTLSNGLTTLTTSGQSPVTGA
                     NQLATGVVSYTDGVNQAAIGSQTLSSGLTTYTNGVASLASGAE"
BASE COUNT         2906 a         1818 c         2238 g         2810 t
ORIGIN      
        1 gacgaccatt gctggtgcag tcctctcctt catcttagga gtaacctgtt ttatttttat
       61 gatcggccta agctcaacaa attttgataa ttgggataca ggtagtgacg gatacattga
      121 tgagactgtg gaatacacgg agtatcaagt tggtgactca gttgattttt ctgacggtct
      181 tcatgtaaca gtaacatcta tgggcaaaga cgatagcata gaactagtgg atagctacta
      241 cagcacagct tatgttgttg aaatggaagt tgaaaattcg accgcaaaag agctctattt
      301 tgatgaatac tatttcagtc tcatggatcc cgttagtcag attcccttta ccctggattt
      361 gcggacctat gatgtgaatc tagttgaaaa gctgaagcca ggagagaaag tacaagtaaa
      421 acttatctac ggtatagata atgaaacaaa ctttggtttt acctacgaag atgccatgtg
      481 gacggaatta gtcgcagaag gtatctaatt gctgtagaaa aagttttaaa tattgcacaa
      541 ttttgcgggt ttatacgccc cttggttcag attgttcggc ttggtaattg tactatatcc
      601 gacttatctg ttatttttac attttaaaaa ggagaacatg tttatccact gttctcctct
      661 tcttttaatg aaaaataccc ccaaaattag cacgtcaatt ttgggggcgt ttttattata
      721 gtttagtaaa ctaaaagtaa gcttatacca aaccttgggc aatcatgcta tccgcaattt
      781 gttcaaaggc tgcgatgtta gcaccggcaa ggtagtctgt accaaggtcg tatttttcag
      841 cagtttcttt ggctgtgttg aagatatttg ccatgatgtc tttaagacgg ccgtctactt
      901 cttcacgagt ccatgacaag cgaaggctgt tttggctcat ttcaagggca gatacggcta
      961 caccaccagc gttggcagct tttgcaagac cgtaaagaac gccattttct ttgtaaactt
     1021 tgatagcatc caggtcagat ggcatgttgg caccttcagc cacacagtaa acgccatttt
     1081 tcacaagggc agcagcttgc tcaccgttga tttcgttttg agtcgcacat ggaagggcaa
     1141 tatcagcctt tccgtcgtag ttccatacag aaccttcgaa gtacttagca gttgcttttt
     1201 ctgtagcgta ttctgtcaaa cgagcgcggc gtttttcttt gatgtccacc aagaggtcga
     1261 agtcaatacc agtttcgtca atgatgtaac catttgagtc tgaaacagaa ataacctttg
     1321 caccaagttc agtcgctttt tgaacagcat attgtgcaac gttaccagaa cctgagataa
     1381 ggactgtttg gtctttgaag gatttaccgt ttgctgccaa catgttatca gtgaagtaaa
     1441 ccaaaccgta accagttgct tctgggcgga tcaatgaacc accaaagcca agaggtttac
     1501 cagtcaagac acctgcatca aactggcgga gacgtttgta ttgaccgtac atgtaaccaa
     1561 tctcgcgacc accgacaccg atgtcaccag cagggacgtc aagtgaaggt ccgatgtgtt
     1621 tttgcaattc agtcataaag ctttggcaga agcgcatgat ctcagcgtct gttttgcctt
     1681 taggatcaaa gtctgaacca cctttaccac cgccgattgg aagaccagtc aagacgtttt
     1741 tgaagatttg ttcaaaaccg aggaacttca agatggattg gtttacagtt gggtggaagc
     1801 gaagaccgcc tttgtaagga cctacagctg agttgaactg aacacggtag ccacggttga
     1861 cttggacatt tccatattta tctgtccatg gaacacggaa gctgatgata cgctcaggct
     1921 caacgatacg agccaggatg ttttcttcga tgtattctgg gtgtgcttca aaaacaggct
     1981 caagtgtaga gaagagctct tctacagctt ggaggaattc tgtttcatgt gggttgcggg
     2041 ctttgactgc ttcaaaagaa gcttggatgt aagctttggc atttgacatg ggatcacctg
     2101 atttcttttt tatttattaa attatctgac aatttaattt atctgataat tattatagca
     2161 ccatgatttt taaatgtaaa gaagaaaatg taagaatttt aaaaaaatta ataaattttt
     2221 attgcaaaat ccgaacgtta gtagtaaatg gggaaaatcc gtttgtttct tatgatataa
     2281 tcaagaaaga atgacctata tggaggataa tatggtttca acagcaacga cactcggtgg
     2341 atttaccttt gataattgtt tgatgaatgc ggcaggggtt tggtgcatga ccaaggaaga
     2401 attggatgcg gttaagaact cgaaggcggg tacttttgta acaaaaacag caaccttgga
     2461 ctaccgcgcg ggtaatccag aaccacgtta tcagaatgtt ccgcttggtt ctatcaattc
     2521 gatgggtttg ccaaatcagg gcttggctta ctatttggat tatctactag aattgcagga
     2581 aacggagtca gaacgcacat ttgttttatc tgtagttgga atgtcgcctg atgagactca
     2641 tgaaattttg agaactgtag aagccagtga ttttaagggg ctgacagagt taaatctttc
     2701 ttgtcccaat gtccctggaa aaccgcagat tgcctatgat tttgaaacga cagaaaccat
     2761 cttgcaggag gtcttcactt actttaccaa accactcggt attaagttac caccttattt
     2821 cgatattgtg cattttgacc aagcagcagc gattttcaac cagtttcctt tgaagtttgt
     2881 caactgtgtc aattcgattg gaaatggtct ctatatcaag gatgagtcag ttgtcatcaa
     2941 gcctaaaaat ggttttggtg gtatcggtgg agagtatatc aagccgactg ctcttgccaa
     3001 tgtccacgct ttttaccaac gcttgaaacc ggagattcag attgttggga ctggtggcgt
     3061 cttgacaggt cgggatgcct tcgaacacat tctctgtgga gctagcgtgg ttcaagttgg
     3121 aacaaccctg caaaaggaag gtgtggcagc ctttggacgc attacggctg aattgcaggc
     3181 catcatggct gaaaaaggct acgagaccat tgaagatttt cgtggcaagc tacagcactt
     3241 agatgactga gatcatctgt tgaagacata aaaagaagct aacattgtac tgttctccca
     3301 gatagtcttt tagggagtcg cgaggggata atgttagctc tttttaaatg gtctgtgaag
     3361 atcggtctga taatcgtttg aggaatggaa tacatagtca gaaacatata tttgtgagat
     3421 ggaattctga ttttatttag attttttagc aaaaatatcg tatactaata ttagaacctc
     3481 tatttataag tgatgtgctt ttatggaaga aatagttttt tgagggactg ctgactgtat
     3541 tttgaacaaa ggttcttaag tagggagtat gagggtaaag gtatggagag aagggcgaac
     3601 tatattcgca gaaaacgata tgcgtcctat cggacaaaga tgcatttgtt ttttatttgt
     3661 ggcttagcga ctattatctt ggcgattatt attgtaagcg agcacctagt tcctaggctg
     3721 aatcaggcct cagaatcaat gcactcagat aattctgttc gcatcatggc tcatggggat
     3781 ttgctctatc acttgcctat tcttcgagga gcagaacagc cagatggaag ttacgatttt
     3841 tctgagaatt ttacctacgt aaagccctgg atagaacagg ctgatttcgc tatcgcagac
     3901 tttgaaggga ccatttctcc agatatcgaa ttagcaggtt acccactttt taatgcgcct
     3961 gccattgtcg ccaacaatat ccatgatgca ggctacgatt tggtggactt agcccacaat
     4021 catatcttgg actcacatct atcagggctg atttccactg tcaatacatt tagagatgca
     4081 ggtgtggata cagtcggtgt ctacgccgaa ggcaatcggg caactgcacc actctacatt
     4141 cgggaagtca atggtatccg tatagctgtc ctagcctatg cttacggttt caatgggatg
     4201 gaggctttgc tgagtcagga ggaatatgat gcctatttat cggattttga taaagaaaag
     4261 atgcgggcag agattgagcg agctgagaaa gaggcagata ttaccatcgt catgcctcag
     4321 acgggtgtag aataccagtt ggagcctacg gaggaacaga ccagtttgta ccatcaaatg
     4381 attgattggg gagcagacat cgttcttggt gggcatccgc atgtagtcga gccgacggaa
     4441 attcttgaga aagatggtca gagaaaatta atcatctatt ctatggggaa ttttctatcc
     4501 aatcagcgta tcgaaagtat ggaagatacc ccaaatgccc agtggacaga aagaggtgtc
     4561 ttgatggacc ttactattca gaaagagaat gggcaaacaa gtattcaaac ggctcaagcc
     4621 catccaacct gggtcaatcg cctttcaaaa gggacttatt caacagatgg atacgaacaa
     4681 ttttcctacc agacttacat tttggaagac tgggtcgctg gtggtcgata ctatggacaa
     4741 ttggatctag atacacaggc gcgtgtggat acagtctatc aagaaatgaa agactttgtc
     4801 aatttggtat ggtgaggaga aagaagatga tagttcgatt aattgcaaca gacatggatg
     4861 ggaccttttt agatggtcag gggaattttg atagagagcg attttcacgg gtcttggatc
     4921 aactagatca gaaaaagatt ccttttgtta tcgctagtgg taatggaatt gggcgattac
     4981 tacaattatg ccaaggattt gaagacagat taatctttgt agcggataat ggagcccatg
     5041 tgtatcaaaa tggcaagaca gtaattcgtc gggctattca gcaagtagaa gtcgaagcaa
     5101 tactgcattt tttcaaaggc cgttgggcgg atgtatgtct gatgttgtcg aacgatggaa
     5161 atatttatat gcaggctggg gcaggtatgc catttgcagg gactgatttg ccaatagaat
     5221 cagctcagat ggttgccttc caaaatcgtg tgacatattt agatgacctc agtgcctatc
     5281 ctaattttga acctatttat aaggttggtc tctgggttcc tgaagcgcgt gtagaatcga
     5341 taacggaaga gtttaaccag gcttttcaag gtcgtcttac tgcagtgaca agtggctatg
     5401 gctccattga tatattgcca gaagggattc ataaggcatg gggcttggaa caagttttga
     5461 aggatctggt aatacaacct gatcaagtca tggccttcgg tgattcagac aatgatattg
     5521 agttattgtc ctatgttggg tattcctatg ccatggaaaa tgcgacagac aaggtcaagg
     5581 cggttgccaa atacatggct ccaagccatt tggaagcagg tgtgcttcag gtcattgaag
     5641 aatatatttt ataagtatat agaaaaaagc tagtgctctc aatccagaga attctagctt
     5701 tttttaattt ccaacactgg tgaacttggt cataccatag cggaagaggt tataattgat
     5761 ggaaaatagg accactacca ccagaggcaa cgccagtccc cacatagaca agtctaagaa
     5821 gtagagggct gggaaataag cggtaaaagc gtaggggaaa acaaatatca gcaggatttg
     5881 aatgattttt ggatagatgt tgagtgggta ttgagccatc tgattgaggg cgaaaattcc
     5941 catagtcaaa ggaaatgatt ccacaatcca aaatgctaga gcagttggac cgagttgaat
     6001 ggctgcatag agtagcccga tacaaatggc aataaagatt agtaaggcta gttttccaag
     6061 actccattcc atacccagtt ccttggaagc ttggactagt gcaagggaac cgatgagggt
     6121 tgttccgatt ccttgtggct gaatttgttc acagataagt tgaaaaagtg ggttcacagg
     6181 catgaggagg aggcgttcaa attcgccttg tcggatgtag cgactaccaa acatccagag
     6241 attgtcaaag aaaatcaagt gaatggagcg tccgacggtc gctattccat agatgaaaag
     6301 catttgaccg tagttaaaac cagcgatttc cttgatattt ccaaagagaa tattgaggaa
     6361 aatcaggtag acaatttgtt caatcaaaga ggagaaaagc ccgatgaaga agtccactcg
     6421 aaaggacatc tgggccatca tgtagacctt gatgttatat agatagaggc tgatatattt
     6481 cttcatgtta acctccaaaa atgacaatac gacgcttggc ctgggaccag agtagagcga
     6541 tgaagcaggc tagaatgggt atccatataa cttgcaatcc caaagcagtt agatcgcttt
     6601 cctgtcccaa taaaatggaa actggaacgt taaccgcata attataggga aggagcttca
     6661 aaaagttttc cacagcctgt ggatagaagc tgagtggcaa aagcgctcca gaagctagat
     6721 tgaaaagccc cattctcaga agcatgactc cccaggcatt gaccgtaaaa aatgctaaga
     6781 agccaaaagc catatccagc aattgaacga taaacatacc gaggatggag ctgactagaa
     6841 agagtaaaat agtttttccg tcaggtagat aaaaatcctg atgaacaagc aagataccga
     6901 taaatacgat agcaaatttt atcagttcca aaagcttatt tccaaggtct ttgaaaaaga
     6961 gggctaggat aaaggaatag ggtctaagaa attctccagc aatgcttcct tttaaaatgc
     7021 tgtaggataa atcttctccg agtgagaaag aatttaaact ggctagtaga ttagcaaaga
     7081 tgatatatgt agtaaaggtt tgtagggtaa agccattgac ttctgattga gtgaagaaaa
     7141 tggttttcca gacatagaga gctaccaaaa tcttcactgc tagtgaaacg aaagtcgcta
     7201 gaaagaaagc cttatactgc atagcattca tcatggtcag gcgggtcatg tagagatatt
     7261 tacgcatgga taccctcctc ataaatctga cggagcatgg tttcaatttc caattccttc
     7321 atggtaacat cttcaatggc ataatgattg aaaacttgct caatcacctg ggcgctggtc
     7381 cagaccttgt tttggtagtg gatttcaaag tgaaaatcat ccttacgaat aaattcccag
     7441 ccctccaatt gcaaatcttt ttcaattggt ttttctgtgg agaagagaat ggtcttgact
     7501 gtggaaaagc gttctttgag gacggtcagt gaaccatcgt agaccttctt gcccttatca
     7561 atgataaaaa tccgctcaca gaggctttcg atgtccttca tatcatggga agtaatcaaa
     7621 aaagtggtct ggtattcttg attcatatag cggatgaagg agcgaatggt ttccttgacg
     7681 ctggcatcca agccgatggt tggttcgtcc aaaaaaaaca acggcaggct ggtggataaa
     7741 gatagccgca aattcacagc gaacccgttg gccgagtgac agattacgga ttggttgctt
     7801 cataatatca gccaagtcca atagctcaat caagacagcc aatcgctctt tgaatacctt
     7861 atctggaaca tcataaattt ttgcgtgaag gataaaagat tcctggacag gcaagttcca
     7921 atccaaatga gatttttgtc caaagagaac gccgatttgc ttattgacag ctttgcggtt
     7981 ttcttgcgga ttgagtccgt tgatacgaac ctgtccttgg tctggataga gaacacctgt
     8041 cagcattttg atggtggtgg atttaccaga accgttggaa ccgatatagc cgatgatttc
     8101 tccttttcct acctgtaagg aaatatcttg aacagcaggg attgactttt tctttggctt
     8161 gaaaaatgcc ttgatagagc cttttagacc gacttcctta tcaatgattg aataggtttt
     8221 tgaaagatgt tctgcctgaa tcatatcata atctcctgtt tgaaaacgtt tgcgtaagat
     8281 aaggatatta taacatattt cccagaaagt ggcggtaaaa agtcaccttt aaggtaacat
     8341 ttttattaga aaatcggtca tatattgagg agattcttgc ttaccatttt taatccatac
     8401 ctgcatgata ccgaagaagg catgtgaaaa atagatgctg ctgtattctt tttcaagtgg
     8461 tgataaatct tctttcccca agcgctccgc tagatcattg gcaacaagaa gtcggacttt
     8521 attgataaga aaattttgga tttcttgagt tccgttatgg gtcagtaggg cagaaagcaa
     8581 tttttcacga tagagtaact cgaaaatttc taaaaatgat gattggcgat tgccatcata
     8641 acggtcaaat atagcctcta atttgtgaaa aagtccctgt tgataggagt caattaattc
     8701 atatttatcc ttatagtggg tataaaaact ggaacgactg ataccagcag tctgggctag
     8761 gtgtgttgtt gtaatgtcat taaatccttg tgattccagg caggttacca tagcttctaa
     8821 aattgcctgt cgagttcgtt ctttccgact atctgttccc ataatttctc cttttgaaca
     8881 tttttagaca cagtgtctaa aaacggttct tatgacttga ttttgaaaat gatacttgta
     8941 taatgtattt tatcagtttt tggacagtat gtctaaagga gttgcttatg ttacgaaaag
     9001 tgaagaatat tttcaaaaaa ccaatgactt tggttaccat tattggtatt gcctgtgtcc
     9061 cagccttata taacattagt ttcctaacct ctatgtggga cccttacggc cgactagacc
     9121 agcttcctgt agcagtgatc aatcaagacc agtcggcgag ctttcaagat aaaaccctta
     9181 ccattggtga tgatatggtg gataatatga aagagagcaa aagtctggat ttccactttg
     9241 tatcagaaaa ggatgcggag aaaggcttgg aagaagggga ctattatatg gtcatcactc
     9301 taccagaaga tttatcagaa aaagcaacca gtctcttgac gaatcagcct gaaccgataa
     9361 cgatttctta tcaaacctcc aaaggtcata gctttgttgc ttctaagatg ggcgaatcag
     9421 ctatggagaa attaaagaca tctgtttcag agacaattac caaaacctac acgactgccg
     9481 tttttgatag tatgagagaa attcaaaccg gcatggtcga ggcggcagat ggaagtcaac
     9541 aattaacaga tggagctagt caattggagt caggcagtca aacactttca aatggattga
     9601 ctactttaac gacttcgggt caatcacccg tgacaggagc aaaccaattg gccaccggag
     9661 ttgtgtctta tacagatgga gtcaatcagg ctgcgatagg cagtcaaaca ctgtccagtg
     9721 gcttaacgac ctacacaaat ggagtagcaa gtcttgcttc aggtgcggag ca
//
LOCUS       DASFSW010000080         9762 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.080, whole genome shotgun sequence.
ACCESSION   DASFSW010000080 DASFSW010000000
VERSION     DASFSW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.080"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..340
                     /locus_tag="U1099_001420"
     CDS             <1..340
                     /locus_tag="U1099_001420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM4867736.1"
                     /translation="CSGSNEIIGTWQAQVADGTELIIEIDKKQIAVYGETSDYVHNGF
                     GFENNMSYYTIEVNEWNIYTIIFPAKDKSIAVLIQPTDFDEPLVGTLIFVMNKNEQPN
                     YLEYGNKYLK"
     gene            578..2575
                     /locus_tag="U1099_001421"
                     /pseudo
     CDS             578..2575
                     /locus_tag="U1099_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            2586..3041
                     /locus_tag="U1099_001422"
     CDS             2586..3041
                     /locus_tag="U1099_001422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867737.1"
                     /translation="MQKEKMTVEEFLQLKKDAQVSLILFIVFGLVLFSSMFYITVIGA
                     RTQMYNSLVTMVFLSVLMSAVRPYVFSKNILEKKQPELKQYSTEGYSVSSAFLKRVFL
                     VYSVAIIVVVFGFASAYATRVETILPDDTLPSLEQKSIIEQKLEGNSNE"
     gene            3034..3420
                     /locus_tag="U1099_001423"
     CDS             3034..3420
                     /locus_tag="U1099_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867738.1"
                     /translation="MSDEYLLSCITNSREKLAKYKRVRNTIMSHNLHAQRSLSGLQSY
                     IEHCQKVIDRIDSQDGYGYLANFRDKLADDIKVLKDYRNFVKDSNASFVDLYQTLNAK
                     IGNLNASIANYKSMYNDGKPVWEWVW"
     gene            3429..3761
                     /locus_tag="U1099_001424"
     CDS             3429..3761
                     /locus_tag="U1099_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867739.1"
                     /translation="MDTNINSIDSQYTYDTIVYDDGQIDGVIQQLNQIIDCLNQISSE
                     IVGLEAQDSSWSGKSKAAYVDLKEFLKSYRKDYQHSIKELKSTVSGLETLLNSIPSAT
                     VLKEIDGA"
     gene            3758..4414
                     /locus_tag="U1099_001425"
     CDS             3758..4414
                     /locus_tag="U1099_001425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867740.1"
                     /translation="MIFSIEALYTLHLLNDHSDDLSFLPLPNGQNLDRKSLLKETLEK
                     GFEDLKELQLVVDNQLTERGVEYGYYLEEYHSSPYHCRVDLSYFCAPAVDEFKRYSIL
                     IKEVDEGVFVIERLASVWFLGFLLETHPILHHLDDKMKDYLMYDWTPKSHFSLLVEHG
                     RSEGLRLAVEQLGTIYSDTLFLEIEESIYEYDLTRELMRSIDSETLQSRLIKDLKVEV
                     "
     gene            4417..4851
                     /locus_tag="U1099_001426"
     CDS             4417..4851
                     /locus_tag="U1099_001426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867741.1"
                     /translation="MSEQIQISLSSQEQIILHALRITELATDITQTIQQVVETIPNFS
                     SQGSFHTIYTTGKNDGFYRYVLKAQELKTLSEVLYRHVETTHQKMVDMDRALAVHITN
                     QFLNSPSTSSEDKQFIREHPEEAVKYIQSEMKKSAPSSGGGS"
     gene            4848..6917
                     /locus_tag="U1099_001427"
     CDS             4848..6917
                     /locus_tag="U1099_001427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867742.1"
                     /translation="MTRFYTNRSDFEQNHWLDSQKSRVVAMLRDIDSVSSTVQSEGLS
                     HLYRSRGEASLGASKPSSTFSQAVDRVDSMLRSAGKIHKDIVQNIDSKFTKGLDKSFQ
                     SLNEVNGSKNPYMSKGKYEKTYTTTDYTSNYGYMTQTHTRQVHYSLSDILDGTKSPIP
                     AAQSLYQDRVKNLREQLRQTDQLTDKQIKELESKSDAELVQEFYPTLESDYSQIKNST
                     WQEENKEALGLVDTGLKILAGIALAAGTVMTGGAALPLALAGGAYLFADGAHSAITGN
                     TLITGTQLSTEERIWAGAEAVVTLATFGSFVAGQGALRAGKQIPTTVQTIGKLAGYAD
                     EGVELGQLASGILSGDGTNATLNYIGGKAFEGIVESSVKGLQSQLSHIEVSSPSQIQL
                     NARQGQNFVDLQTVEFGKIASKVENNITIKMTVDGVEQKIRVDAIGFDEAGNIRIQEY
                     TTAQNGLKISRQNLLEDLSKYGGTIVGAGKGDFVGGVEIPKGTRIDVVSQKTGNVTIG
                     SLDTVYSVKDFKAEMEQLPHINLSKKVEDGLVSTNNGSRTSPHHVYSVEEIATHLAMF
                     DDGAVKIMSFESYDYGLQNYGATIGHPDGHYVMPKFVYEKAVRASNGNPRVLEGLLGL
                     EQGYLGNNPVVIDIQRLKNLRMPSGNEPGAWQGLWEPGGYTQGGIPEAVTDQLSESDY
                     TIDEVFK"
     gene            6933..7793
                     /locus_tag="U1099_001428"
     CDS             6933..7793
                     /locus_tag="U1099_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867743.1"
                     /translation="MKVLRGTDFIIYELNGQYSINWYRGRERFSYPIDEDLVKKALLT
                     SKDGKEVMFYVEHGRWPEEDELEHYNESNVITHRGNNFIVYEENGKYEMHFMTGGFQD
                     REVIYPISKELMERAFESPQDAYEVKIYLMTGHWPDKSQEELDRNFLRQFPEQILKNP
                     TIPKTLFSEYEFTKLLKQAIETVLQTSKIDSLGIVEEHLELLLLDPIKWQEETEIIHL
                     QLLQEKLNNYIHFIESKQYVESYGDDFTEKVINLTFQYAPSDNGLAFLMQVQKVLQPT
                     DIRLKVVVPE"
     gene            8119..8736
                     /locus_tag="U1099_001429"
     CDS             8119..8736
                     /locus_tag="U1099_001429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867744.1"
                     /translation="MDAIGFDEAGNIRIQEYTTAQNGLKISRQNLLEDLSKYGGTIVG
                     AGKGDFVGGVEIPKGTRIDVVSQKTGNFSIDSTPNYIQVGRYTTELSKIDLPLEEKVI
                     RLQEFYSDLSDKTDINVPSDPQYVVAVRDGWVEYDWPKNLGYQEGTVQSITRDSGLPD
                     QWDRFGHMGGGNFSDIPSDGPYTYSQRAIPYVENPNAYHKGTFIR"
     gene            8733..9014
                     /locus_tag="U1099_001430"
     CDS             8733..9014
                     /locus_tag="U1099_001430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867745.1"
                     /translation="MSYTDFIKLYQESLKVGVQLIIGAQKSSLLKTDLSIKYIKENLV
                     TAIVAQRLYDQSIVQHKMTSREETLKVDEVYLYHDQDYQKVKISKQVVE"
     gene            9065..9673
                     /locus_tag="U1099_001431"
     CDS             9065..9673
                     /locus_tag="U1099_001431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867746.1"
                     /translation="MGLTGCNKEVEIRTELAPMYEVLDQQSIENFDIFSIEDNLRIYG
                     MESAKAFKTDLAISPDGQFEGMSYDLSVSETEGYPTTYIDGNLKINTTSEVLSDRKMM
                     FEEFHFSEDYFNSLELVKVLDHPNTYLKEITYQEKTPSEYTKMLIDLYDLTTPRMANI
                     EISKVRADEKTFAYILTYSLYDETNISIKRVFSFVMEDKTDE"
BASE COUNT         3004 a         1663 c         2356 g         2739 t
ORIGIN      
        1 ttgtagtggt tctaatgaaa tcatcggaac atggcaagca caggtagcag atgggacaga
       61 gctgataatt gaaattgaca aaaaacagat tgcggtatat ggtgagacct cggactatgt
      121 tcacaatgga tttggttttg aaaataacat gagttattat actatagagg ttaatgagtg
      181 gaacatctat acgattattt ttcctgcaaa agataaatca attgccgtct tgattcagcc
      241 tacagacttt gacgaaccgt tggttggaac cttaattttt gtcatgaata aaaatgaaca
      301 gccaaattat ttggaatatg gcaataaata tcttaaatag tcaaacttat atattaccaa
      361 tcatagatta tttctatcca gacaagatta ccttgtctgg attttttggt ataatggaaa
      421 agtagaaata ttcttcattt gtgcataaac ggttaggcta ggcttggtct aaagcggagc
      481 tggaggatga gatggaagta gaatcgcttt attttatttc tatttcgaaa cgatgtgagc
      541 agctatgcta tttttcaaaa acctcaagaa aggtatgaag ccatctggtg tggtggatga
      601 ccagagctgg tccaactccc gcttcaagct ggcgctcaag gtggcagatc ggagcgactc
      661 catggagatg cttaagacgc cagatgcagc tgagattacc caggtgggtc gtggctacct
      721 ccaagtcgga aacaatgaag tgtatgaact cttccagtct gcctggtctg gggctgatta
      781 ccagccagat aaggatgagc aggggattga ggaccatacc atctatgcca tcaatgacat
      841 tggtcaatat gagattctca acgaagacct gtcaggtctg gaacaggctg aatccatcaa
      901 ggaggttccg agtgagcttc aagccatcgt aagtaagctg aaagccttga cagagcgcct
      961 gtccatccag gccctgccgc aaccatggtt gcctccgctg tctaaggaaa tcttcctgca
     1021 agatctacga ccacaaggtt tcaaggactt gtggggacag gcaagtggtt tggttgcacg
     1081 cttggggatg gtggatatac cgagtcgcca gagccaagaa gtcttggaac atgactttga
     1141 aaaggatgga cacatcgccc tcttctctag tccaggtatg gggaaatcga cctttgttca
     1201 aacggtattg gttgatttat tccgacaaaa caatccagat cgtcttcaca cttatctgct
     1261 cgattttggg accaatggat tattggctct aaatgattat ccacatgttg cggagtattt
     1321 cttggttgaa gagactgaaa aattacagaa gttgataaaa cgactgcaag atgaaatacg
     1381 agaaagaagg aaattattca ccgattcctc cgttcataat atcgaaatgt atcgcaagtt
     1441 aggagatagg aagttagctg atattgttat tgcggtggac agctatgatg gtcttaaagg
     1501 ttcggaagtc gaagtggcga tcgatacctt gcttcaaact cttgcaagag atggaatggg
     1561 gcttgggatc tatttgatcc taaccgctag tcgaacaggg gcactcaaaa acaatttgta
     1621 tagtaatttt aaacgaagaa tagccctgaa aatgaatgat gatagtgaag cgcgagcaat
     1681 cgttggaaga actgctttgg ttattgaaga tgtgccgggt agagggctta ttcaagtgga
     1741 tgatcctgag ttgattcagt tagcaatgcc ggctaaaggt gagaatggtc tcgagatgat
     1801 cgaagcatta cgtcatgaat cccaagaaat ggctaagaat tggaatggag cattacctca
     1861 aggcattgtc attgttccag aggttcttga tgaacagact tttttacaaa tggtggagac
     1921 agagaaagca atcgcttcag attttattcc gattggttta gaatttgaag aggtacacac
     1981 aatctcgttg ccgattccta agttaaaaca tctattgatt ttgactgaaa aggaacagca
     2041 taagttagta gcagttcgcc atcttttaaa tatgctttgg catgttcgaa aggggcagtc
     2101 tgtgacgctg tttgatgtga cagatgaata tggccgattt gctgggaaag tgaagcaata
     2161 ttattcttct tctgaactaa ctgaaaatgt agtggaagag ttgaaaatga gagttttaca
     2221 tgctcgtaaa caaaaagaac aattgaatga atttgtcatc atcagtgatt taccagcctt
     2281 tatgacagtt gatggcatgt cagataatga ttttgcactt ctgtatgaag agggacagcg
     2341 tgttggtttg cacttgattg ttgtagctaa caagacttat atgtcgctgt cgagtggtat
     2401 tcaacgcttg attaaacaaa aattggatac ggtactgatc gcgatgaaga tgagcaacca
     2461 gtcggtggtg gcacgaagcg aggttggacg ggaggcagag ttggccatcg atgaggtata
     2521 cctccactat caagaccaac aaatcaagtt gaaaattacc aaagaaatag aataggagag
     2581 ttaggatgca aaaagaaaaa atgacagtag aagagttttt acaactgaag aaggatgctc
     2641 aggtatcctt gattctattt attgtttttg ggcttgtctt gttttcaagt atgttttata
     2701 taacagtgat tggtgcaaga acacaaatgt acaattcact tgtgacgatg gtgtttctat
     2761 ctgtattgat gagtgcagtc agaccctatg ttttttctaa aaatatctta gaaaaaaagc
     2821 aacctgagct gaagcagtat tcgacagagg gctactcagt ttctagtgcc tttctaaaaa
     2881 gggtttttct agtttattcg gttgcgatta ttgtggttgt tttcggtttt gcctctgcct
     2941 acgcaactcg ggttgagacc atccttcccg atgatacctt accaagtctg gaacagaagt
     3001 ctattatcga gcaaaaattg gagggcaaca gtaatgagtg acgagtattt gcttagttgt
     3061 attaccaata gtcgtgaaaa gcttgcaaag tataagcgag taaggaatac tattatgagt
     3121 cataatcttc atgctcaacg tagcctatct ggtctccaat cttacattga gcattgtcag
     3181 aaggtcattg ataggatcga tagtcaggat gggtacggtt atctggcaaa ctttcgtgat
     3241 aagttagcgg atgacataaa ggttttgaag gactatcgga atttcgtgaa ggattcaaat
     3301 gcatcctttg ttgacttata ccagacattg aatgcaaaga taggaaattt gaatgcttca
     3361 atcgcaaact ataaaagtat gtacaatgat gggaaacccg tgtgggaatg ggtttggtag
     3421 gaggtgctat ggatacaaat attaatagta ttgacagcca atatacgtat gatacaattg
     3481 tgtacgacga tggtcagatc gatggtgtta ttcaacagtt gaatcagatt attgattgcc
     3541 tgaatcagat ttcttcagag attgtagggc tggaagcaca ggatagtagc tggtctggaa
     3601 aaagcaaagc tgcctatgtt gatttaaaag agtttctcaa gtcctatagg aaagattatc
     3661 agcacagtat caaagagctc aagtcaacgg tttcgggatt ggaaaccctt ttgaactcca
     3721 tccctagtgc aacagtattg aaggagattg atggcgcatg atattttcaa ttgaagcatt
     3781 gtacaccctc catcttttga acgatcattc ggatgactta agttttcttc cgcttccaaa
     3841 tgggcaaaac ttagaccgca agtccttgtt aaaggaaacg ctagaaaaag gatttgagga
     3901 tttgaaagag ttacaattgg tggtggataa tcagttgact gagcgagggg tggagtatgg
     3961 ctactatctg gaagagtacc attcgtcccc ttaccactgc agagtggact tatcctattt
     4021 ctgtgctccg gctgttgatg agtttaagcg atattctata cttatcaaag aggttgatga
     4081 gggggtgttt gtcattgagc gtcttgccag tgtttggttt ttggggttct tgctggaaac
     4141 tcatccgatt cttcatcatt tggatgataa gatgaaggat tatttgatgt acgattggac
     4201 accgaaatcc cattttagtc ttctggttga acatggtcgg tcagagggac ttcgtttggc
     4261 ggtggagcaa ctagggacaa tttacagcga tacactcttt cttgaaattg aagagagtat
     4321 ctatgagtac gatttaacac gagaactcat gcgctcgatt gatagtgaaa cactgcaatc
     4381 aaggcttatc aaggatttga aagttgaggt ataagaatgt cagaacagat tcaaatttcg
     4441 ctcagctctc aggaacaaat cattttacat gctttgcgca ttacagagtt ggcgacagat
     4501 attacgcaaa ctattcagca agtggttgaa acaattccta atttttccag ccaaggttcc
     4561 ttccatacga tttatacaac tgggaaaaat gatggtttct atcgctatgt gttgaaagcc
     4621 caagaactta agacactttc agaagttctc tatcgtcatg tagaaacgac tcatcagaaa
     4681 atggtggaca tggatagagc cttggctgtg catatcacca accagttctt aaacagccct
     4741 tcaacgagtt ctgaagataa gcagtttatc cgagaacatc cagaagaggc ggtgaagtat
     4801 atccagagtg agatgaagaa atcagcacca tcatcaggag gtggatcatg acacgttttt
     4861 atacgaatcg ctcagatttt gagcagaacc actggctaga ttctcaaaag agtcgagtcg
     4921 tcgctatgtt gcgggatatc gattcggtct cttcaacagt gcagtcggaa ggtctttctc
     4981 atctctatcg ctctcgtggt gaggctagtt taggtgcaag caagccgagt tctactttta
     5041 gccaggctgt tgaccgtgtc gatagcatgt taagaagcgc tggaaagatt cacaaggata
     5101 ttgtccaaaa tattgatagt aagtttacca agggcttaga caagagtttt caaagcttga
     5161 acgaagttaa tggcagtaag aatccttata tgtctaaggg gaagtatgag aaaacttata
     5221 cgactactga ctatactagt aattatggct atatgaccca aacgcatacc agacaggtgc
     5281 attacagtct ttcagacatc cttgatggca cgaaaagtcc aattccagca gcccagtcat
     5341 tatatcaaga ccgagtgaag aaccttcgag agcagctccg ccagaccgac caactaacgg
     5401 ataaacaaat caaagagttg gagtccaaga gtgatgcgga gttggttcag gagttctatc
     5461 cgactctgga gtcagactat agtcaaatca aaaatagtac ttggcaggag gagaacaagg
     5521 aagctttagg cttggttgat acaggtctca aaattttggc gggcattgcc ctagcagcag
     5581 gaacggttat gactggaggt gctgcccttc cgcttgcact tgcaggtggc gcgtacctct
     5641 ttgcggatgg agctcactct gcgattacag gcaacacctt gattactggt actcagttga
     5701 gcacggaaga gcgtatctgg gctggagctg aggctgttgt gacattggct acttttggta
     5761 gctttgtggc aggtcaaggt gccttgcgag caggtaaaca aatcccgaca accgttcaga
     5821 ccattggtaa attggctggc tatgcggatg agggtgtgga actgggacaa ttggcatctg
     5881 gtatccttag tggagacggt acgaatgcga ccttgaatta catcggaggt aaagcatttg
     5941 aaggcattgt agagagctct gttaagggac tccaaagcca actttctcac attgaagttt
     6001 caagcccaag ccaaattcag ctcaacgctc gtcagggtca gaactttgtg gacttgcaaa
     6061 cggttgagtt tggtaaaatc gccagcaagg tcgagaataa catcacgatc aagatgacgg
     6121 ttgatggcgt tgagcagaag atacgagttg atgcgattgg ttttgacgag gcaggaaata
     6181 tccgcatcca ggaatacaca actgcccaga atggtttgaa aatcagccgt cagaacctct
     6241 tggaagacct gtccaagtac ggtggcacca ttgtcggcgc aggcaagggc gactttgtcg
     6301 gcggtgttga gatacccaag ggcacacgga ttgatgtcgt gtcgcagaag acggggaatg
     6361 tgacgatagg atctctggat accgtttatt ctgtaaaaga ctttaaagct gaaatggagc
     6421 aattgcctca tataaatctg tccaaaaagg ttgaagatgg cttggttagt acgaataacg
     6481 ggagccgtac gtcgcctcac catgtttatt cagtagaaga aattgcaact catcttgcta
     6541 tgttcgatga tggagcagtg aaaattatga gctttgaatc atatgattat gggttgcaaa
     6601 attatggtgc gacgataggc catcctgacg gtcattacgt aatgcctaaa tttgtttatg
     6661 aaaaagctgt aagggcatct aatggtaatc caagagtttt agagggacta ttgggattag
     6721 aacaggggta cctaggaaat aatccagttg ttatagatat tcagcgctta aaaaacctcc
     6781 ggatgccttc tggtaacgag cctggagctt ggcaaggttt gtgggaacct ggaggctaca
     6841 ctcagggggg gattcctgag gcagtgactg atcagcttag tgagtcggat tatacaatag
     6901 acgaagtatt caaatagaaa ggtcagatat atatgaaagt gttacgaggg actgacttta
     6961 taatatatga attaaatggg caatatagca ttaattggta tcgtgggcga gaacgattct
     7021 cttatccaat agatgaagat ttagtaaaaa aagctctgtt aacatctaaa gacgggaaag
     7081 aggtcatgtt ttatgtagag catgggcgct ggccagaaga ggatgagtta gagcattata
     7141 atgaatctaa cgtaattaca catagaggga ataattttat tgtctatgag gaaaatggta
     7201 aatatgagat gcactttatg actggcggat ttcaagaccg agaggtcatc tatcctattt
     7261 ctaaagaact tatggaaaga gcttttgagt cgcctcaaga tgcctatgaa gtaaaaattt
     7321 acttaatgac aggccattgg ccagataaga gtcaagaaga attggataga aatttcttaa
     7381 ggcagtttcc agagcaaatt cttaagaacc cgactattcc aaagactctc ttttcagagt
     7441 atgagtttac gaaattattg aaacaagcaa ttgaaacagt attacagact agtaaaattg
     7501 atagcttagg aatagttgaa gaacatttgg aactcttgct tctagaccca atcaagtggc
     7561 aggaagaaac agaaattatt catttgcagt tattgcaaga aaagttgaac aactacattc
     7621 actttatcga aagcaagcag tatgtggaaa gttacgggga tgactttaca gaaaaagtta
     7681 tcaatcttac tttccagtat gctccgtcag acaatggtct tgctttcctt atgcaagttc
     7741 aaaaagttct acagccgaca gatattcggt tgaaggttgt agtgccagag tagagtttta
     7801 agtccagaca gggtcacctt gtctggattt tttggtataa tggaagggta gaaatatttt
     7861 tcatttgtgc ataaacggtt aggctagact tggtctaaag cggagcctag gagtgggaat
     7921 gagattggag ttgttatctc tcgcatccat tttgaaacaa gttgagccgc tctgctatta
     7981 ccacaaattc agctcaacgc tcgtcagggt caggactttg tggacttgca aacggtcgag
     8041 tttggtaaaa tcgctagcaa ggtcgagaat aacatcacga tcaagatggc ggttgatggc
     8101 gttgagcaga aaatctgagt ggatgcgatt ggctttgacg aggcaggcaa catccgcatt
     8161 caggaataca cgactgctca gaatggtttg aaaatcagtc gtcagaacct cttggaagac
     8221 ctgtccaagt atggcggcac cattgtcggc gcaggcaagg gcgactttgt tggcggtgtt
     8281 gagataccta agggcacacg gattgatgtc gtgtcgcaaa aaacggggaa tttttcaata
     8341 gatagcactc ctaactatat ccaagtagga cgttatacta cagaactttc taagatagac
     8401 ctgccacttg aggaaaaagt tataagactt caagagtttt attcagatct ttcggataag
     8461 acagatatta acgttccatc ggatcctcaa tatgtagtgg cggtacgcga cggttgggtt
     8521 gagtatgatt ggccaaagaa tttgggttat caagaaggta ccgttcagtc aattacaaga
     8581 gattcaggat tgccagatca atgggatcgt tttggacata tgggaggagg gaacttctcg
     8641 gacatcccta gtgatggtcc ctatacctat agtcagcgtg ccattccgta tgtagaaaat
     8701 ccgaatgctt atcataaagg aacctttata agatgagtta tacagacttt ataaaactct
     8761 accaagaatc actcaaagta ggggtccaac tgataattgg tgcacaaaaa tcatcgttac
     8821 tcaagacaga tttatctatt aagtatatta aagagaatct ggtgacggca atcgttgctc
     8881 aaagacttta tgatcaaagt attgtacaac ataaaatgac aagtcgtgag gagactttga
     8941 aagtagatga agtgtatcta tatcatgacc aagactatca aaaagtaaaa attagtaagc
     9001 aggtggtgga gtaatgaatc gaataaaaaa actaatgatg tgtattttag gaggtgttac
     9061 aataatgggt ttgacaggtt gtaataaaga agtagagatt agaacagagt tggcaccgat
     9121 gtatgaggtg ttggaccagc aatctataga gaattttgat attttttcga ttgaagataa
     9181 tttgaggata tatggtatgg agagtgccaa ggcatttaag actgacttgg ctattagccc
     9241 agatggacaa tttgaaggaa tgagttatga tttgtcagtt tctgaaacgg aaggctatcc
     9301 aacgacttat atagatggaa atttgaaaat taatacgact agtgaagtgt tatccgatag
     9361 aaaaatgatg tttgaagaat ttcatttttc agaagactat ttcaatagtt tagagttagt
     9421 aaaagtactg gaccacccca atacttatct gaaagagatt acctaccaag agaagactcc
     9481 ttcggaatac acaaagatgc tgatagattt atatgattta actaccccaa gaatggcaaa
     9541 tattgaaatt agtaaagtaa gggctgacga aaaaacattt gcatatatat taacatattc
     9601 attatatgat gagactaata tttcaattaa acgtgtcttt tcttttgtga tggaggataa
     9661 aacagatgag tagggctgat atcaatcgtc gggtggaggt atctgccgat attgcagaca
     9721 gtgtttataa gtttgaagag tattttgtaa agaatagcga ta
//
LOCUS       DASFSW010000081         9689 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.081, whole genome shotgun sequence.
ACCESSION   DASFSW010000081 DASFSW010000000
VERSION     DASFSW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.081"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..258)
                     /locus_tag="U1099_001432"
     CDS             complement(<1..258)
                     /locus_tag="U1099_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4867747.1"
                     /translation="MAIKNQKKYKGVYCDKNGKIFYQADLGVDPVTGKRVQKKARKNQ
                     YGKPFTTMKEAYDELVRIRYEFNNNLSIENYNITFESYMNTI"
     gene            complement(261..509)
                     /locus_tag="U1099_001433"
     CDS             complement(261..509)
                     /locus_tag="U1099_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEM4867748.1"
                     /translation="MKTVTYKNLMNIGFPEHTARNIIREAKRIAVKKFEEARKVDHNA
                     VQLSKSPFDNRRLGIAPKNIVEELIGISLSEDNLESEN"
     gene            complement(779..1003)
                     /locus_tag="U1099_001434"
     CDS             complement(779..1003)
                     /locus_tag="U1099_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867749.1"
                     /translation="MYQRIRDLREDHDLTQKFVANLLSFSHTNYAKIERGEVALTADV
                     LVQLSKLYNVSTDYLLGLTDCPDRIKHKIK"
     gene            complement(1250..2146)
                     /locus_tag="U1099_001435"
     CDS             complement(1250..2146)
                     /locus_tag="U1099_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEM4867750.1"
                     /translation="MVIELKNEQLTVQFSEFGGALSSIKDKDGVEYLWQGNPEYWSGQ
                     APVLFPICGSLRNDWAIYRQAERPHFTGIIPRHGLVRKENFDYCKIDDSTVSFGFKAN
                     EKTLKEYPYHFVLNIIYKLHGSTITTEYQVTSSEIERSIPYFIGGHPGFNCPLEDDEK
                     YEDYYLEFSEYETCSIPKSFPDTGLLDLQDRTPFLENQNKLDLDYSLFSHDAITLDQL
                     KSRSVSLKSKKSNKGIKVDFKDFPHLIIWSTTNQSPFIALEPWSGLSTSLEESDILEE
                     KQGVTFVEPSQTSIKSFDITIL"
     gene            complement(2234..3640)
                     /gene="lacG"
                     /locus_tag="U1099_001436"
     CDS             complement(2234..3640)
                     /gene="lacG"
                     /locus_tag="U1099_001436"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM4867751.1"
                     /translation="MSKTLPKDFIFGGATAAYQAEGATKTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEFGVNGIRISIAWSRIFPTGFGKVNEKGVEFYHNLFAE
                     CHKRGVEPFITLHHFDTPEALHSNGDFLNRENIDHFVNYAEFCFKEFTEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDLAKVFQSHHNMMVSHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDKNNPDDVRAAELEDIIHNKFILDATYLGGYSEKTMEGVNHILSLNGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGPTEIIHNGKGEKGSSKYQIKGVGRR
                     EAPVDVPKTDWDWIIYPQGLYDQIMRVKRDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAIEDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKQVAETQVIE"
     gene            complement(3706..5409)
                     /locus_tag="U1099_001437"
     CDS             complement(3706..5409)
                     /locus_tag="U1099_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM4867752.1"
                     /translation="MKGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFKWSADIETLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVSVVLLYAIELLVHNALGVNVAESIGKLLAPLFQ
                     AADGYVGISIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEANLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILTPIVNVWIFKFFIDSLGMNSFTANLPWTTPAPLGIVLGTNFQVLSF
                     ILAAVLIVVDVAIYYPFLKVYDQQILEEERTGKSSDELKEKVAANFNTAKADAILEKA
                     GATETVESKEITEQTNVLVLCAGGGTSGLLANALTKAAKEYGAPVTAAAGSYGGHREI
                     LPQYQLVILAPQVASNYEDLKVETDKLGIKLAKTEGAQYIKLTRDGQGALDFVKQQME
                     N"
     gene            complement(5409..5726)
                     /locus_tag="U1099_001438"
     CDS             complement(5409..5726)
                     /locus_tag="U1099_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM4867753.1"
                     /translation="MNREEITLLGFEIVAYAGDARSKFLEALKAAQAGDYAKAEELIA
                     AGGECLNDAHNAQTSLLQKEAAGEDLAFSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(5754..6734)
                     /gene="lacD"
                     /locus_tag="U1099_001439"
     CDS             complement(5754..6734)
                     /gene="lacD"
                     /locus_tag="U1099_001439"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM4867754.1"
                     /translation="MVLTAQKRSLMEKVSDKNGVISALAFDQRGALKKMMAKHQTEEP
                     TVEQIETLKTIVSEELTKYASSILLDPEYGLPASKARADEAGLLLAYEKTGYDASTTS
                     RLPDCLDDWSVKRLKEQGADTLKFLLYYDVDGDEQVNRQKQAYIERIGSECKAEDIPF
                     FLEILSYDEGIADNTGVEFAKVKPRKVNEAMKVFSDERFGIDVLKVEVPVNMNFVEGF
                     GDGEVVYTKEEAAQHFRDQEASTHLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVLC
                     GRATWAGSVQVFIEQGEEAAREWLRTTGFQNIDELNKVLEKTASPWTAKV"
     gene            complement(6737..7669)
                     /gene="lacC"
                     /locus_tag="U1099_001440"
     CDS             complement(6737..7669)
                     /gene="lacC"
                     /locus_tag="U1099_001440"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEM4867755.1"
                     /translation="MILTITMNPSVDISYPLEQFKLDTVNRVAKTHKTPGGKGLNVTR
                     VLSQLGDDVIASGLLGGKIGEFIESELDKSSVKHSFYKIAGETRNCIAILHDGNQTEI
                     LEQGPTISEEEATGFLNYFEKLVPDLGIVAISGSLPKGLDTNFYSRMVGICNKYNIPV
                     VLDCSGEALLNVLDGQEKPTVIKPNTEELSQLVGTEVTDDIDSLKSVLHNSLFDGIEW
                     VIVSLGSKGAFAKHHNVFYKVSIPKIEVVNPVGSGDSTVAGITSALVHNDSDENLLKK
                     ANVLGMLNAQEALTGHVNLNNYDNLFNQIEVTEV"
     gene            complement(7681..8196)
                     /gene="lacB"
                     /locus_tag="U1099_001441"
     CDS             complement(7681..8196)
                     /gene="lacB"
                     /locus_tag="U1099_001441"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEM4867756.1"
                     /translation="MKIAVGCDHIVTNDKIAVVDYLKTQGYEVIDCGTYDNIRTHYPI
                     FGKKVGEAVASGEADLGVCICGTGVGINNAVNKVPGIRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIHAEYKPTEENKKLIEKIQHVETLNQGQADPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(8230..8655)
                     /gene="lacA"
                     /locus_tag="U1099_001442"
     CDS             complement(8230..8655)
                     /gene="lacA"
                     /locus_tag="U1099_001442"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEM4867757.1"
                     /translation="MAVIIGADKAGTELKETVKSYLIELGKEVVDVSKDGQDFVDVTL
                     AVAKEVTSNDSNLGIVVDAYGVGPFMTATKIKGMIAAEVSDERSAYMTRGHNNSRIIT
                     IGSEIVGPGVAKNIAKGFVEGEYDGGRHQIRVDMLNKMC"
     gene            complement(8893..>9689)
                     /locus_tag="U1099_001443"
     CDS             complement(8893..>9689)
                     /locus_tag="U1099_001443"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEM4867758.1"
                     /translation="EYLLENSLSLGDIKGIGMGSPGKIDFEKGTVTGAYNLGWSKEQN
                     IREQFEEAFGRPFYIDNDANVAALGERWKGAGDNAPDVIFVTLGTGVGGGIIAQGQLL
                     HGVNGSAGEIGHMVVEEDGFPCTCGNIGCLETVASATGIVNIAKSFASQFDEPSELRR
                     LILEHQVTAKDVFDYAKKNDSLGQKIVWQFANYLGKSLSQLANALNPNYIVIGGGVSA
                     AGDFLLDKVKEEFDRFAFPTVRNSTKLALATLGNDAGVIGAASLVL"
BASE COUNT         2970 a         2034 c         1480 g         3205 t
ORIGIN      
        1 aatggtattc atataacttt caaatgttat attataattt tcaatactaa ggttattatt
       61 aaactcatac ctaattcgaa ccagttcatc atacgcttct ttcattgtag taaatggctt
      121 accatactga ttctttctag ctttcttttg aactcgtttc cctgttactg gatcaacacc
      181 aagatcagcc tgataaaata tcttgccatt cttatcacaa taaacacctt tatacttttt
      241 ttgatttttt atagccatct tcaattctca ctttctaaat tgtcttcaga gagtgaaatt
      301 ccgattaact cttctactat atttttagga gcaataccaa gtcttctatt gtcaaagggc
      361 gatttactta attgtaccgc attatggtca actttgcgag cttcttcaaa cttttttact
      421 gctattcttt ttgcttctct aatgatgttt cttgccgtat gttcaggaaa accaatattc
      481 atcaagtttt tatatgttac tgttttcata cctccactcc tgtcaaaaaa attatttcct
      541 cagtaggtat gattggttaa atcactcatg ggcatctcac ccgctgtcct tttacgttgg
      601 cagcctgaac gttcagaagt atcattgtcc ttatttgtgg tcatggcaga tagagaacca
      661 ctctatttta tagctaataa ttatcgctcc tatcacggcg tatctcacta aattgctcga
      721 caaataagaa agtcccacct cggtttattc aattgtcaat gaacaaatca aggagagact
      781 attttatttt atgttttatc ctatctggac aatctgttaa ccctaatagg taatctgtac
      841 taacgttata gagtttagat agctgcacaa gaacatctgc cgttaaggca acctctcctc
      901 tctcaatttt tgcataattt gtatgagaaa aggaaagtaa atttgcaaca aacttttgag
      961 tgagatcgtg atcttccctc aaatctctta tccgttggta catctagctt tctcctaaga
     1021 aaagtatatg tcactttcag aaaaaagctg ggaaattgtc ataatatgac agtaattatt
     1081 gtttagttaa aaccaacatt tatttttgta aatatttcaa tactattttg aatatattct
     1141 agattgacta ctacctcaga ccaccttaca ttagattata caaatttata ttggattttt
     1201 gtaaatttct aaagtcccta tctttatatt ccattcagat tactttaatt tacaaaatag
     1261 taatatcaaa tgatttgatg ctagtttgac ttggttctac aaatgtaacc ccctgcttct
     1321 cttccaatat atctgattcc tctaatgatg ttgataatcc actccatggc tctaaggcta
     1381 taaatggact ttgattagtg gtggaccaaa taattaaatg tggaaaatct ttaaaatcca
     1441 ccttaattcc cttattactt tttttagact tcaaactgac actacgtgac tttagttgat
     1501 ctagggtaat agcatcgtgt gaaaacaaag aataatccaa atctaattta ttttgatttt
     1561 caagaaaagg ggttctatct tgtaaatcaa gtaatccagt atctgggaaa ctctttggaa
     1621 tgctacatgt ttcatattca gaaaattcta aataataatc ttcgtatttc tcgtcatctt
     1681 ctaaagggca attaaatcct gggtgacctc caataaagta aggaatagaa cgttcaattt
     1741 cactgcttgt cacctgatat tccgttgtta tcgttgaacc atgtaattta tatataatat
     1801 tcaacacaaa atgataagga tattctttaa gtgttttttc attagcctta aaaccaaagg
     1861 acactgtgct gtcatctatc ttacaataat caaaattttc ctttctaact aatccatgtc
     1921 gtggaataat tccagtaaaa tgaggtcttt ctgcttgtct gtatattgcc caatcattcc
     1981 tcaaacttcc acaaatcgga aataaaactg gcgcttgtcc actccaatat tctggattac
     2041 cttgccaaag gtattcaact ccatctttat ctttaataga agataaagca ccaccaaact
     2101 ccgaaaactg tactgttaac tgttcatttt ttaattcaat aaccatattt ttccctccgt
     2161 tatacaaaac aagagcgtca gtataacacc cacactctta tcttgcgtta gaaattttta
     2221 agaagaatat tatctattca ataacctgag tttctgctac ttgtttatac cagtaggctg
     2281 attttttcgg ataacgttcc tgtgtctcaa aatctacata gaagagacca tagcgttttt
     2341 cataaccatt tgaccatgag aaaacatcca tcaaggacca aataaagtat cctttaacat
     2401 tggctccatc ttcaatcgca tctgaaatga cttctaagtg tttcttcacg taatcaatac
     2461 gaccatcatc atagacagta ttgtctacaa attcatcttt ataacccaaa ccattttcag
     2521 tgatataaat tttcttataa tttgggtagt ctcgttttac acgcatgatt tggtcatata
     2581 gaccttgtgg ataaataatc cagtcccaat ccgtcttagg tacatcaact ggggcttcac
     2641 ggcgtccaac acctttaatt tgatatttag agctaccttt ctcaccttta ccattatgaa
     2701 taatttctgt tggcccatca aaggcttgca tccaatcact catgtaatag ttaataccaa
     2761 ggaaatcgtt caaatcttta gctgcttcta gcgcttgaaa atcttcatca cgaagatcaa
     2821 gctcaccacc atttaatgac aggatatgat tgacaccttc catcgttttc tctgagtacc
     2881 cacctagata agttgcatca agaatgaatt tattatggat aatatcttca agttctgctg
     2941 ctctgacatc atcaggattg tttttatcaa atggatattt tgttggtaga gcatgtacta
     3001 ctccaatttc acccttatat ccaccatctt taaacagttt cacagcacga gcatgagaaa
     3061 ccatcatgtt atgatgtgat tggaagactt tagcaagatc gtatttgata cctggtggga
     3121 atttaccaac aagatattgt ccatcaccaa ttggtccaat ttcattaaag gttgtccaat
     3181 aattaacttc tgtaaattct ttaaaacaga attctgcata gttaacaaaa tggtcaatat
     3241 tttcacggtt taagaaatcg ccatttgaat gtaaagcttc aggagtatca aagtggtgaa
     3301 gagttataaa tggctcaacg ccacgcttat gacactcagc aaaaagattg tgatagaatt
     3361 caacaccttt ttcatttact tttccaaacc cagttgggaa aatacgggac caagcgattg
     3421 aaatacggat tccattaaca ccaaattctt cagctaactt caagtcaaca ggatagcgat
     3481 tatagaaatc acttgctggc tcagccgtat accagtaatt atcttctagg tatttgtccc
     3541 aagcaactgg acctttaccg tctgttttag tagcaccttc agcttgatat gctgctgttg
     3601 caccaccgaa aataaaatct ttaggtaatg tttttgacat ttgtttttcc tcttcatcta
     3661 aaaagggaag ttgagttccc ccaaccttcc ctaatgttgt ctattttaat tttccatttg
     3721 ttgtttaaca aagtccaatg cgccttgtcc atcacgagtc aatttgatgt attgtgcacc
     3781 ttctgtctta gctaatttga taccaagttt atctgtttct actttcaagt cttcatagtt
     3841 tgacgctact tgaggagcaa ggataacgag ctgatattgc ggcaagattt cacggtgacc
     3901 accatagctt cctgctgctg cagtaactgg agcaccatat tctttagctg cttttgtcaa
     3961 agcatttgca agaagaccac ttgttcctcc acctgcacag agaaccaaaa catttgtttg
     4021 ttcagtaatt tcttttgatt caacagtttc agttgctcct gctttttcaa gaatggcatc
     4081 agcttttgct gtattgaagt ttgcggctac tttttctttc aactcatctg aagatttacc
     4141 agttcgttct tcttcaagaa tttgttggtc ataaaccttc aagaatgggt aataaatggc
     4201 aacatcaaca actatcaaaa cagcagctaa gatgaatgat agaacctgga agttagtacc
     4261 tagtacaatt cctaggggag ctggtgttgt ccaaggtaag tttgcagtaa aactgttcat
     4321 tcctagacta tcaataaaga atttgaaaat ccaaacgtta acaattgggg ttaagataaa
     4381 aggaatgaag aaaattgggt tcaataccag tggagcaccg aacaaaattg gttcgttaac
     4441 tccaaagaaa gtaggaacaa cagacgcacg tccaatcgct ttattacgtt ttgatttcgt
     4501 caaccacata aacatgaacg gaacaaccaa tgttgcacca gtaccaccca tcgtaacgat
     4561 gaacatttga gtacctgatg tcaaaatctt atcagcatgt tcccctgctt gaagcaattg
     4621 taagttagct tcagcattgg cataggtaat ggctgcaata gctggctcga caattgaagg
     4681 cccgtgaata ccaacaaacc agaagaaagc aaaggcacca aagataatgg aaataccaac
     4741 ataaccatct gctgcttgga acaatggagc taataattta ccaattgatt ccgcaacatt
     4801 tacacctagt gcattatgta caagaagttc aatagcataa agcaatacga ctgagacagt
     4861 aaatgggatt aagtctttaa atacttgtga aatattcggt ggaacctctt ctggcatacg
     4921 gatggtaaca ttgtttttca cacaaacctt gtaaacatta accgtaataa aggctgcaat
     4981 gaaggctgta agcaaacctt tagtacccat aaatccagtt aagaaaccac catcttttgc
     5041 tggattagca gccatcaaca ggaatccaac aatggctgca agcattgtag aaatgaagtt
     5101 aatttgattt gtgctaggca atgagcggtt cattgaatcg gttaatgctt ttgcagttgt
     5161 accagcaaca aacaatccaa gaattcccat tgaataattg tatggagtca tcagaagtgt
     5221 ttcaatatca gcactccatt tgaatcccca agcattagga acataagcaa ttaagatgaa
     5281 aatacttgag aaaagaatga ccggcatacc agcaataaag ccatcacgaa tagcacggag
     5341 gtaaatattc cgagaaatct tctcaaagaa aggtttccct ttctcaattt gagcaatcaa
     5401 ccctttcatt attttgctcc tctcttatac aactcaatca tatgtttcat taggtcttga
     5461 agtaagattg tagtcatcaa gtgatcttga ccatgcatta aggtaacact aaaggcaaga
     5521 tcctcaccag ctgcttcttt ttgtagaaga cttgtctgag cattgtgtgc atcatttaag
     5581 cactcaccac cagctgcaat caattcttca gctttagcat agtcgcctgc ttgagctgct
     5641 ttgagagcct ctaaaaactt agagcgtgca tctccagcat aagcaacaat ttcaaatcct
     5701 aaaagagtga tttcttctct attcatgata ttttcctccg aaaattttta tgattatact
     5761 tttgcagtcc aaggactagc agttttttcc aaaactttgt tcaattcatc aatattttgg
     5821 aatccagttg tacgtaacca ttcacgagct gcttcttccc cttgttcaat gaaaacttga
     5881 actgatccag cccatgttgc acgtccacaa agaacaccat tgaattttgc acctgattct
     5941 gccgcaaata ctaaggtatc ttggaataat ttagcagaaa cacccgcact taaatagata
     6001 taaggtaggt gtgttgaagc ttcctggtca cggaagtgtt gtgctgcttc ttccttagtg
     6061 tagacaactt caccatcacc aaaaccttca acgaaattca tattgactgg aacttcaacc
     6121 ttaagaacat caataccgaa acgctcatct gagaagactt tcatcgcttc attcacttta
     6181 cgaggtttta ctttggcgaa ctcaacacct gtgttgtctg caataccttc gtcataagac
     6241 agaatttcaa ggaagaacgg aatatcttcc gccttacatt cagaaccaat acgttcaatg
     6301 tatgcttgtt tctgacggtt tacttgctca tcaccatcta catcatagta tagcaaaaat
     6361 ttcagtgtat cggcaccctg ttctttaagg cgtttgactg accaatcatc caaacaatct
     6421 ggtaaacgac tcgttgtgct tgcatcgtat cctgtttttt cataagcaag taatagtcct
     6481 gcttcgtctg cacgagcttt ggacgctggt aaaccgtact ctggatcaag aagaatagag
     6541 gacgcatact ttgtcaattc ttctgaaaca attgttttca atgtttcgat ctgttcaacc
     6601 gttggttctt cagtttgatg tttcgccatc atttttttga gtgccccacg ttggtcaaaa
     6661 gccaaagctg agataacgcc atttttatcg ctgacttttt ccatcaatga acgtttttgt
     6721 gctgttaata ccattattat acctctgtta cttctatttg attaaataaa ttatcgtaat
     6781 tgttaagatt gacatgtcct gttagcgctt cttgagcatt caacatacca agtacatttg
     6841 cttttttcaa taaattctca tcactatcgt tatgaactaa agcagatgta ataccagcta
     6901 ccgttgagtc tcctgagcca acaggattga ccacttcaat tttagggata gataccttat
     6961 agaaaacatt atgatgtttt gcaaaggctc cttttgaacc aagtgaaaca atcacccatt
     7021 caatcccatc gaaaagagaa ttgtgaagaa ctgatttcaa actatcaata tcatcagtca
     7081 cttccgttcc tactagttga gaaagttcct cagtatttgg tttaataact gttggttttt
     7141 cttgtccatc aagtacattt agaagggcct ctcctgaaca atctaataca actgggatat
     7201 tatatttatt acaaattccc accatacgag aataaaagtt tgtatcaaga ccttttggga
     7261 gactacctga gatagctaca attcctaaat ctggaaccag tttttcaaaa tagtttaaga
     7321 accctgttgc ttcttcttca ctaatcgtag gaccttgttc aagaatttct gtttgattgc
     7381 catcatgtaa aatagcaata cagtttcttg tttccccggc tatcttataa aacgaatgtt
     7441 ttacacttga cttatctagt tcactctcta tgaactctcc aattttaccg cctaacaagc
     7501 cacttgcaat cacatcatct cccagttggg aaagaactcg agtaacattt aagccctttc
     7561 caccaggtgt tttgtgcgtt ttagcaacac gattcacggt atctaatttg aattgttcaa
     7621 gcggataaga aatgtcaact gacggattca ttgttattgt cagaatcata accttacctc
     7681 ttagtcgtga tattcaccac gatcccattt ttctaggaat tcatcaaaga agtgtggatc
     7741 agcttgtcct tgattgagag tttcaacatg ttgaattttt tcaatcagtt ttttgttttc
     7801 ttctgttggt ttatattctg catgaataaa cgcttcaata atatctgtca tcaataaacc
     7861 accagtgatt ttaccaccaa aaccaatgac gttagcgttc aattcttctt tagcatagat
     7921 agctgatgtc atatcacgaa caagagctga gcgaatacca ggaactttat tgacagcatt
     7981 gttaatgccg acgcctgtac cacagataca tacaccaagg tctgcttcac cactcgcaac
     8041 tgcttcacct acttttttac caaagattgg gtaatgcgta cgaatgttat cgtaagtacc
     8101 acagtcaatc acttcataac cttgagtttt caaatagtct actaccgcaa ttttatcgtt
     8161 tgtaacaata tggtcacagc ctactgcaat tttcatttgt tttctcctct aattttaact
     8221 tttgaatttt tagcacattt tattcaacat atcaacacga atttggtggc gacctccatc
     8281 atattcacct tcaacaaatc ctttagcgat atttttggca acaccaggtc ctacaatctc
     8341 agaaccaatg gtaatgatac gtgagttatt gtgaccacgt gtcatataag cagaacgctc
     8401 atctgaaact tccgcagcaa tcatcccttt gattttagtt gctgtcatga aaggtcctac
     8461 accatacgca tcaactacga ttcccaagtt tgaatcgtta gaggtcactt ctttagcaac
     8521 tgctaaagtt acatctacga agtcttgacc atctttactc acatcaacaa cttctttacc
     8581 aagttcaatc aagtatgatt taactgtctc tttaagttct gtacctgctt tatcagcacc
     8641 aataattact gccattttct ttctcctgtt acattagaaa ataattgaat gtagctacat
     8701 cgacgataaa tttttgcaaa actgtttgaa ataacgcttt cattatttct acaccattat
     8761 aataccacca ttaaaaacaa aagtcaacag ttttatgttt tttcttgtgt gttttaaact
     8821 atttgtgttt gatttgtttg tttttaaaaa aaataaaaac accaacaaac ttaatatttg
     8881 ttggtataac tattataaaa ctaggcttgc agctccaatc actccagcat cattccctaa
     8941 agtagctaaa gctagtttag tagaattcct aactgttgga aaagcaaatc tatcaaactc
     9001 ttcttttact ttatctaaaa gaaaatcccc tgcagcagaa acaccaccac caataacaat
     9061 ataattggga ttaagtgcat tagccaattg actcaatgat ttacctaagt aattggcaaa
     9121 ttgccataca attttttggc caagagaatc gtttttctta gcgtaatcaa aaacatcctt
     9181 tgcagttact tgatgttcta aaattaacct tcttaattca cttggttcat caaattgact
     9241 agcaaagctt tttgcaatat tgacaatccc tgtggcgcta gcaacagtct ccaaacaccc
     9301 aatattccca caagtacatg gaaatccatc ttcttcaaca accatatgac caatctcacc
     9361 agcacttcca ttaacaccat gaagtaattg gccttgagca ataattcctc cacctacacc
     9421 tgttccaagt gttacaaaaa taacatcagg tgcgttatct ccagccccct tccatcgttc
     9481 accaagcgcc gcaacattag catcattatc tatataaaaa ggacgaccaa atgcttcttc
     9541 aaattgttct ctgatatttt gttctttcga ccaccctaag ttataagctc ctgttactgt
     9601 tcctttctca aaatcaatct tgccaggact tcccatgcct atgcccttaa tatcgcctaa
     9661 agataagcta ttttctaata gatattctt
//
LOCUS       DASFSW010000082         9448 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.082, whole genome shotgun sequence.
ACCESSION   DASFSW010000082 DASFSW010000000
VERSION     DASFSW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.082"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            97..3090
                     /locus_tag="U1099_001444"
     CDS             97..3090
                     /locus_tag="U1099_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEM4867759.1"
                     /translation="MKFMFGKRWLSIFAILLLTLSNCSPTIGYALENMSESTSSSVIS
                     SAEETVSKSETTTEMSQSSEEIVEMKQAEENTETNRSDAPSSSQDLADNSEESVQKIL
                     HYEDEYVTADLNAYFGNGVENFSVTLPRMKNSGKWDSEREYVNQLSQYLVNQNLQPAD
                     VQQIEISDSQIVKEGYKPRLTVNFKTSLGLRGGLVTKENLRLVSTNTDGSLVFHDAEF
                     HFDTTGTLIGLTYEGILGKDIHVVMVKPLAEKTTVTLGQYVQMQLDGYVLEEEEEQTA
                     SNVPLNARLKVKIFRSDASGIFTILSPIDSFREEKTFDVLSTWKPSFNLLGIESEKFD
                     IVKMNYRQAHQSKTDSLMYFNGEFPVASFHQESGEEESNVLEIYLQNRMLEGTGMILG
                     LRTRMLGYRSFSGFRSVTNATVSNSSVDQAHITQSKRIDYLGDGGNNPDTSVDDRIGT
                     DTHELYRMYLDMTGTKEPFDTLIVVDRSTSMTDPMNSVDTQARYLAVYKALNGTAGRQ
                     GLLSKLVGFHPENQVAIVGFQGYPGYPSGDQDSTVIANWGRSTSVALSNIQPPYNNGT
                     NYTAGLRTAGVVLDQNQSSRKKVMIFISDGVPTFAFVNGVRYGNGTISGNNPYYTRDW
                     TLNYFNSWIGKYPKLPIYTLGISSEFGNSDNLSANPYVLNHMSSQTGGFYSHVADSQA
                     LERTLQKIVDDTKLSLVSINDQLSQYVSYYAEQPDVKVVQTNRQTGQVTTLYQNGAVT
                     SQGVGILQSVTHTAVNASDSTGKVDLNFVDNFKIDDTYTYTISYNVKVTDRAYEEKKT
                     SGYNAHGDSGTDYTGNSTSSGQAGFRSNKVASLTYIRNEQPYEKEYDHPVVQVDTRQF
                     TLQKSDSKETNLLLENVRFELRLSDKKTVFASGETDSRGNITFTDLRRAKTYYLYETK
                     AKDGYTLPEDPWTVDVAVDGTITIRDQLGYTVSKSGEQYRVVNHKLYQLPSTGGYGPA
                     VYILLGVMVITLASYLFYEKRKQIA"
     gene            3117..4757
                     /locus_tag="U1099_001445"
     CDS             3117..4757
                     /locus_tag="U1099_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4867760.1"
                     /translation="MKRIFTLLTVLLTVLGMNARPVFAASNTDQAELKITNIEGNPTV
                     TLYKIGEGVYNATNDSFIRFDYASGVKLTETGPTAEEITNIANGILDNTIQAKASKVL
                     QVTNGTATYRATGAGVYIAILTGATDSRSFNPILLAASYNGEGKLTGGSVDSKAKYLY
                     GQTSVAKSTLPSIDKTVSGVTADADKNSASLGQVLTYSLNVTLPSYPTQAKNKTIFIS
                     DTMSEGLTFDYASLTISWNGQTLIADTTGQFKTQGDILIAKAVKVGNGFNLAFVYDNL
                     NEIAPVVTYKGIINDKAVVGGTGNANKAEFFYARNPNSGNTYEDPNNKPNPQNDNTIV
                     NKEDSETVYTYQIAFKKVDSITKAPLAGAVFGIYSDAETKQLVDIVTTNADGYAISTK
                     VGKGTYYLKEITAPTGYSLNTNVYTAEASWTSATTTSSASSTRSEYTSVVGEAKDSTQ
                     VGWLKHNVFYKLDNKPEGTDVQAAYLKATTTTAQNTTVTTTNPTAGAGTVSIADVPNT
                     KLGELPSTGGMGTYLFTFIGVLVLTVGLGLYFTKNKKA"
     gene            4825..5676
                     /locus_tag="U1099_001446"
     CDS             4825..5676
                     /locus_tag="U1099_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM4867761.1"
                     /translation="MKQRAGIRYIFLILVGILILLYPYISSSWNRYRDQQLISSHREW
                     VDTQPEQEKEKMWQVAQSYNNELGIQPVPDAFSLREGIHDPKYETVLNASQDGMMGYV
                     EIPKIDIELPIYHYTSDSVLQKGAGHLFGSALPVGGDGSHTVVSAHRGLPNAEMFTNL
                     PQLEKGDQFYFHVLGEILAYEVDWIQVVKPNQVSSLSGIEGEDYATLITCTPYGINTE
                     RILVRGKRVPYDKKDYEKAQSSQKKVDEIYLAVQVVCGVLGFLLSLLIILFVKKRDQK
                     RKNKESM"
     gene            5737..7893
                     /locus_tag="U1099_001447"
     CDS             5737..7893
                     /locus_tag="U1099_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEM4867762.1"
                     /translation="MEEKYMKIAQELGVSLKQIDTVLSLTAEGNTIPFIARYRKDVTG
                     NLDEVVIKSIIDRDKALTALADRKATVLAKIEEQGKLTDQLRQAIEEAEKLADVEELY
                     LPYKEKRRTKATVAREAGLFPLARLILQNVADLEEQAADFICEGFDTAQSCLAGAVDI
                     LVEAISEDNKLRAWVYHEVQTNSNLTSELKDQEADEKEVFQIYYDFSEKVAKMQGYKT
                     LAINRGEKLGVLKVSFEHNVDKMIRFFELRFPQSNSYIKDVIQQAIKKKILPAMERRI
                     RTELTEEAEEGAIQLFSKNLRNLLLVSPLKGKVVLGFDPAFRTGAKLAVVDQTGKLLT
                     TQVIYPVEPAGQRQIAQAKKDLADLIGQYQVEIIAIGNGTASRESEAFVADLLKDFPD
                     VSYVIVNESGASVYSASELARYEFPDLPVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVNQKSLSESLDFVVDTVVNQVGVNVNTASPALLAHVAGLNKTISENIVKYRE
                     ENGALTSRQQLKKVPRLGDKAFEQAAGFLRIPDATNFLDNTGVHPESYKAVENLLELL
                     AIDHLDEAAQEKLKQVAIADTAEKIGVGQETLKDIVADLLKPGRDLRDDFEAPVLRQD
                     VLDVKDLVVGQELQGTVRNIVDFGAFVDIGVHEDGLVHISRMVKRNRDKNGRQQALPH
                     PSEVLAVGEIVTVWVVEVDIKRNRIGLSLLKPNGSE"
     gene            7880..8314
                     /locus_tag="U1099_001448"
     CDS             7880..8314
                     /locus_tag="U1099_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEM4867763.1"
                     /translation="MDLNKYVQEVSLQDFGKEFRHVAIWNRRLRSTGGRFFPRDGHLD
                     FNPKHLEEQGLEVFRKIVRHELCHYHLYFEKKGYRHGDKDFKELLAAVDGLRYAPKLE
                     QVAKPSLLYTCQSCGQVYQRKRRIDLKKYRCGKCRGKLTLKE"
     gene            8384..9145
                     /locus_tag="U1099_001449"
     CDS             8384..9145
                     /locus_tag="U1099_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867764.1"
                     /translation="MTQKREERLIELYEKGVLTKEEARACFKEMNQEPDFLFVEEKVK
                     LNFTLPSFKVFASSKLKQSYSFEGIESLFLKLSEGRLTLAKSKNNQISVEICYNQDAP
                     EDKLPRLYVEKKGLYFHSSLACRLTINLPQEWMSVLDLELGQADARLDYLPFEDISIR
                     SSRDKKQQDIRLTTCGGYPQHLYVQLAQAPLTLQTGKGQGIRGQIESQSGHVLVNRKK
                     KNSPYQFEKSGNDLLFLKAQTGQGGFYVKGIKDVN"
     gene            9135..9407
                     /locus_tag="U1099_001450"
     CDS             9135..9407
                     /locus_tag="U1099_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEM4867765.1"
                     /translation="MSIELYKVRQGKIVSGVLAGIAHKLGWESWVTRAIFIAGLLLGK
                     SFLLLIALYIAAAYFLPYKEDRDAERYGTGPRKIKEAEKINKSWFE"
BASE COUNT         2896 a         1718 c         2192 g         2642 t
ORIGIN      
        1 gacgtttaga agagaattgt gatatagtac caaaagattc tttgctaaat catttgatta
       61 atatagcaaa tttcgattct tatggaaagg aggaatatga aatttatgtt tggaaaaaga
      121 tggctttcca tctttgcgat tttgttactt actttgagta actgcagtcc aacaattggt
      181 tatgcgttag agaatatgag tgagtcaaca agtagttcag ttatttcttc agcggaagaa
      241 actgtttcta aaagtgaaac aacgacagaa atgagtcaat catcagaaga aattgttgaa
      301 atgaaacaag cagaggaaaa taccgaaacg aatcggtcag atgcacctag ttcttctcag
      361 gatttggcgg ataattctga agaatctgtt caaaaaatcc tgcactatga agatgagtat
      421 gtgactgctg atttaaacgc atactttggt aatggtgttg aaaactttag cgtaactcta
      481 cctcggatga aaaactcggg taagtgggat tctgaaagag agtatgtaaa tcaacttagt
      541 caatatctcg tgaatcagaa tttacagcct gctgatgttc agcagataga aatttcagat
      601 agtcagattg tcaaggaagg ttataaacca aggttgacag ttaatttcaa gacttctctg
      661 ggattgcgtg gcggtttagt gaccaaagaa aatcttcggt tggtttcgac caatacagat
      721 ggctccttgg tttttcatga tgctgaattt cactttgata cgacaggtac cttgatagga
      781 ttgacctatg aaggaatact tggaaaagat attcatgtgg taatggttaa gccattagca
      841 gaaaagacaa cagtgacatt gggacaatat gttcagatgc agttagacgg gtacgttctc
      901 gaagaggaag aagaacaaac agctagtaat gttcctttaa atgcacggct aaaagtgaaa
      961 atttttcgtt cagatgcttc aggtatcttt actatcttaa gtccaataga ttcatttcga
     1021 gaagaaaaga catttgatgt cttatccact tggaaaccta gttttaattt attgggaatt
     1081 gagagcgaga agtttgatat agtcaaaatg aattaccgtc aggctcatca gtctaaaacc
     1141 gatagcttaa tgtatttcaa tggcgaattt cctgttgcat catttcatca agaaagtggt
     1201 gaagaagaga gtaacgttct agaaatttat ctccagaacc gaatgctcga aggtactggt
     1261 atgattttgg ggcttcgcac gaggatgtta gggtacagat cttttagtgg atttagaagt
     1321 gtaacgaatg cgactgtatc gaattcgtcg gtagatcagg cccacatcac gcagtctaaa
     1381 cggattgact atttaggtga tggtgggaat aatccggata ctagtgttga tgaccgtatt
     1441 ggtacagata cgcatgaact ctatcggatg tatctagata tgacggggac aaaggagccc
     1501 tttgatactc taattgtggt agataggtca accagtatga ctgatccgat gaatagtgta
     1561 gacacacagg ctcgctatct agccgtttat aaagccttaa acggaactgc agggcggcaa
     1621 gggctgctta gtaagttggt tggttttcat ccggaaaatc aagtagcaat agttggtttt
     1681 caaggttatc caggttatcc aagcggagat caggattcaa cggttattgc taactgggga
     1741 cgttctacct cggttgcact gagtaatatt cagccacctt ataataacgg aaccaattac
     1801 acggctggtt tacgaacagc tggtgttgtt ctagatcaga accaaagtag tcggaagaag
     1861 gtaatgattt ttatttcaga tggggtgccg acctttgcat ttgtgaacgg tgttcgatac
     1921 ggaaatggta ccattagcgg aaataatcct tattatacga gggattggac gcttaactat
     1981 ttcaacagtt ggataggtaa gtatcctaag ttacctatct ataccttggg gatttctagt
     2041 gagtttggaa atagtgacaa cttgtctgct aatccatatg tattgaatca catgtctagt
     2101 cagacgggag gattttattc gcatgttgcc gattcacaag cacttgaacg gactctccaa
     2161 aagatagttg atgatacaaa attgtctctt gtttccatca atgaccagtt aagtcaatat
     2221 gtatcttatt acgcggaaca gccggatgtg aaagtggtac aaacgaatcg tcagacgggc
     2281 caggttacaa cgctctatca gaatggagcg gtaacgagcc agggagtagg tattttacaa
     2341 tcagtaacac atacggctgt taatgctagt gattctacag ggaaggttga tttgaatttt
     2401 gtagataatt ttaaaattga tgatacctat acttatacaa tttcttataa tgtaaaagtg
     2461 acagatcgtg cttatgagga aaagaagacg tctggttata atgcacacgg ggattctgga
     2521 actgattata cgggaaacag taccagttct gggcaggctg ggttccgatc taacaaggtg
     2581 gcatcattga cctatattcg aaatgaacaa ccctatgaaa aagaatacga ccatcctgtg
     2641 gtgcaagtag atacacgtca atttacccta caaaaaagtg atagtaagga gactaatctc
     2701 ctgctagaaa atgttcgttt tgaattacgg ttatctgaca agaagacagt ctttgcaagc
     2761 ggtgagacag atagtagggg aaatataacc tttacagatt tacgtcgagc aaaaacgtac
     2821 tatctatatg aaaccaaggc taaagatggc tatactctcc cagaggatcc ttggacggta
     2881 gacgttgccg ttgatgggac gattaccatc agagatcaac taggttatac agtaagtaaa
     2941 agcggtgaac aatatcgcgt tgtgaaccat aaactttatc aattaccatc tacaggtggg
     3001 tatggtcccg cggtctatat cctactaggt gtgatggtta ttacattagc aagctatctt
     3061 ttttatgaga agagaaagca gattgcttaa ttttttaaag ctcaaaggag aagaaaatga
     3121 aaagaatatt cactctttta acagttcttt taaccgtttt aggaatgaat gcacgtccag
     3181 tatttgctgc atcaaatacg gatcaagcag aattaaaaat taccaacatt gagggaaatc
     3241 ctacagttac cctttataaa atcggtgaag gtgtatataa tgcaacgaat gactcgttta
     3301 ttcgtttcga ttatgcaagt ggagttaaac taacagaaac tggaccaact gcagaagaaa
     3361 ttacaaacat tgctaatggt attttagaca atacaattca agccaaagca tcaaaagttc
     3421 tccaagttac aaatgggact gcaacctata gagcgacagg tgcaggggtt tatattgcta
     3481 ttttgacagg agctacagat agtcgttcct tcaacccaat cttgcttgca gcgtcttaca
     3541 acggtgaagg taaattgaca ggtggaagtg ttgattcaaa agctaaatat ctttatggtc
     3601 aaacttctgt agccaaatca actcttccaa gtattgacaa gacagtttca ggagtgactg
     3661 cagatgctga taaaaattct gcttcactag gtcaagtatt gacttattcc ttgaatgtga
     3721 cgcttccaag ctatccaact caggctaaaa acaagacaat ttttatttca gatacaatgt
     3781 ctgaaggttt gacatttgat tatgcaagct tgacaatttc ttggaatggt caaacattga
     3841 ttgctgacac tactggtcaa tttaaaactc aaggggatat tttgattgct aaggcagtta
     3901 aagttggcaa tggctttaat ctggcatttg tctatgataa tttgaatgaa attgcacctg
     3961 tagtgaccta taaaggtatc atcaatgaca aggctgttgt tggtggaaca ggaaatgcca
     4021 acaaggctga gttcttctat gcgcgtaacc ctaacagtgg taatacgtac gaagatccaa
     4081 ataataagcc aaatcctcaa aatgacaata ctatcgtaaa taaagaagat agtgagacag
     4141 tctacactta ccaaatcgcc tttaaaaaag tagattctat cacgaaagca cctcttgctg
     4201 gtgcagtttt cggaatttac tctgatgcgg aaactaagca attagtagat attgtaacta
     4261 caaatgcgga tggttatgct atctcaacaa aggttggtaa gggaacctac tatttgaaag
     4321 agattactgc accaacaggc tactcattaa acaccaatgt ttatactgct gaagcaagct
     4381 ggacctcagc aacaacaact tcaagtgcaa gctcaactcg tagtgaatac acctcagttg
     4441 tcggtgaagc aaaggatagc acacaagttg gttggttgaa acacaatgtc ttttataaat
     4501 tggataataa accagaaggt accgatgtac aagctgccta tttgaaagca acaactacta
     4561 cggcacaaaa tacaactgtg acaacaacta atccaactgc tggtgcgggt acagtatcta
     4621 ttgctgacgt accaaatacc aaattaggag aattgccatc aacaggtgga atgggaacat
     4681 acctattcac atttatcggt gtcttagtgc ttacagttgg cttaggtttg tactttacta
     4741 aaaataaaaa agcataagta acagaattaa taaacaagta tggagggaag gcgttgcctt
     4801 ctctacattt tttaggagaa ggacgtgaaa caaagagcag gtatcagata tattttcctt
     4861 attttagtgg ggatactgat tttattgtat ccgtatatca gtagcagttg gaatcggtac
     4921 cgtgatcaac aattaatcag ttctcataga gagtgggtag atactcagcc agaacaagaa
     4981 aaggaaaaaa tgtggcaagt tgctcaatcc tataataatg agttagggat tcagcctgta
     5041 cctgatgcct tttccttacg tgaggggatt catgatccta agtatgagac agtgttaaat
     5101 gcctctcagg atgggatgat ggggtatgta gaaattccaa aaatcgatat tgaattaccc
     5161 atctatcact atacaagcga cagtgtctta caaaaaggag ctggtcacct atttggtagt
     5221 gccctgcccg ttgggggaga tggtagtcat acggttgtat cagcccatag agggttaccg
     5281 aatgcagaaa tgtttaccaa tcttccgcaa ttagaaaaag gagatcaatt ctatttccat
     5341 gttttgggag agattcttgc ttacgaagtt gattggattc aagttgtgaa gccgaatcag
     5401 gtaagttcac tgtccggtat tgagggagaa gattatgcga cattgatcac ttgtacacct
     5461 tatggaataa atacagaacg gattttggtt cgcgggaaac gagttccgta tgataagaag
     5521 gattacgaaa aagcccaatc tagtcaaaag aaagttgatg agatttacct tgctgttcaa
     5581 gtggtttgtg gtgtattagg attcctccta tctctcctaa tcattttatt tgtgaaaaaa
     5641 cgtgatcaga agagaaaaaa taaagagagc atgtagttta tctacgtgct ttttctattg
     5701 agtcatttgt accgctttca tgctataata gtagctatgg aagaaaaata catgaaaatt
     5761 gctcaggaat tgggcgttag tttaaaacag attgatacgg ttctctcttt gacggctgaa
     5821 ggcaacacca ttccctttat cgctcgttat cggaaggatg tgacggggaa cttggacgag
     5881 gtggtcatca agtctatcat tgatcgtgat aaggccttga ctgctctcgc tgaccgtaag
     5941 gctactgtcc ttgccaagat tgaagaacag ggaaaattga cagaccagct tcgacaggct
     6001 atcgaggaag cggaaaagtt ggcagatgtg gaagaactct acctgcctta taaggaaaaa
     6061 cgtcggacca aggcaacggt ggcgcgtgag gctggactgt tcccgctggc tcgattgatt
     6121 ttgcaaaatg tggctgactt ggaagaacag gctgcggact ttatctgcga gggctttgac
     6181 actgctcagt cttgtttggc tggtgctgtg gatattttgg tggaagctat ctcagaagac
     6241 aataagcttc gggcatgggt ttatcatgaa gtacaaacca attcaaacct gacttcagag
     6301 ctaaaagatc aagaagcaga tgagaaggaa gtctttcaga tttactatga cttttctgag
     6361 aaagtagcga agatgcaggg ctataagacc ctggccatta accgtggtga gaaattaggt
     6421 gttctcaagg tttcttttga acacaatgtg gataaaatga tccgcttctt tgagttgcgt
     6481 tttccacagt ctaatagcta catcaaggat gtcattcagc aggctattaa gaagaaaatt
     6541 ctgcctgcca tggagcgccg tattcgaaca gaattgacag aagaagccga agaaggagct
     6601 atccagctct tctctaaaaa cctccgcaat ctacttctcg tatctcctct aaaaggcaag
     6661 gttgttcttg gttttgaccc agcctttcgg acaggggcca agctagcagt ggtagaccag
     6721 actggaaaac tcttgactac acaagtcatc tatcctgtgg agccggctgg tcagcgacaa
     6781 attgctcagg caaaaaaaga cttggcagac ctgattggac aataccaagt ggaaatcatt
     6841 gctatcggaa atggaacggc cagccgtgag tctgaggcct tcgtagctga tttactcaag
     6901 gacttccctg atgtttctta cgtcatcgtc aatgaaagcg gagcctctgt ctactcagct
     6961 tctgaactgg ccagatatga gtttccagac ctacctgtgg aaaaacgctc tgccatttcc
     7021 atcgcccgcc gtctgcaaga tcctctggct gagctggtca aaattgatcc caagtcaatc
     7081 ggcgtcggcc agtaccagca cgatgtcaac cagaagtctc tgtctgaaag tctggatttt
     7141 gtggtcgata cggtggtcaa ccaggtcggt gtcaatgtca atacggccag tcctgccctt
     7201 ttggctcacg ttgctggtct taacaagacc atttcggaga acatcgtcaa gtaccgcgag
     7261 gaaaatggtg ctctgacctc tcgtcagcaa cttaaaaagg tgcctcgtct gggtgacaag
     7321 gccttcgagc aggcagctgg tttcttgcga attcctgatg cgactaactt tttggacaat
     7381 actggcgtgc accctgagtc ttacaaggct gtggaaaatc tgttagaact tctagctatt
     7441 gaccacttag atgaggctgc gcaggaaaaa ttaaagcagg ttgctatcgc agatacggca
     7501 gaaaaaatcg gtgtcggtca agaaaccttg aaggacatcg tcgcagactt gctcaagcca
     7561 ggtcgtgatt tacgggatga ctttgaagca ccagtcctcc gtcaagatgt cttggatgtc
     7621 aaggatttgg tagttggaca ggaattgcaa ggtacggttc gcaatattgt ggactttgga
     7681 gcctttgtgg atatcggtgt ccacgaggat ggtctagtcc acatttcacg catggtcaaa
     7741 cgcaatcggg ataaaaatgg acgccaacaa gccttaccgc atcctagcga agtcctcgct
     7801 gttggggaaa tcgtaaccgt ctgggtggtt gaagtggaca tcaaacgtaa ccgaatcggt
     7861 cttagccttt tgaaaccaaa tggatctgaa taagtatgtg caagaagtct ccttgcaaga
     7921 cttcggtaag gaatttcggc atgtagcaat ctggaacagg cgattacgct caacaggtgg
     7981 tcgctttttt ccaagggatg ggcatttgga tttcaatccc aagcacttgg aggagcaagg
     8041 tttagaagtc tttcggaaga ttgtccgcca cgagctctgc cactaccatc tctactttga
     8101 gaagaaaggc tatcggcatg gggacaagga cttcaaggaa ctgctggctg cggtggacgg
     8161 cctgcgctat gctcccaaac tggaacaagt tgccaagcct agtctgctgt atacctgtca
     8221 gtcctgtggg caagtctacc agcgcaagcg ccgcattgac ctaaaaaaat atcgatgtgg
     8281 gaagtgtagg ggaaaactta ctttgaaaga atagagaatc agtcttcggg ctgatttttt
     8341 tgtggagaaa tgtgatatac tagagtagaa ttgaggtgag tctatgacgc aaaagagaga
     8401 agaacgctta attgagttat atgaaaaagg tgtcttgacc aaggaagagg cgcgtgcctg
     8461 ttttaaggaa atgaatcaag aaccagactt tctttttgtg gaagaaaaag taaaattgaa
     8521 ttttaccttg ccaagtttta aggtctttgc ttcctccaaa ttgaaacaat cttatagttt
     8581 tgaagggatt gaatcactct ttttaaaatt atctgaggga cgtctaactt tggctaagtc
     8641 taaaaataat cagattagtg tggagatttg ctacaatcaa gatgcaccag aagataagct
     8701 tcctcgtctt tatgttgaaa aaaaggggct ttattttcac agtagcttgg cctgtcgttt
     8761 gacaatcaac ttgcctcagg aatggatgtc agtgctggat ttggaacttg gtcaggcaga
     8821 tgctcgcttg gattacttac catttgaaga tatttctatc cgcagttcaa gggataaaaa
     8881 acaacaggat attcgcttga cgacttgcgg tggttatccc caacacttgt atgtgcaact
     8941 agctcaggct cccttgactt tacaaactgg caaaggccag ggaattcggg ggcagattga
     9001 gtcgcaatca ggtcatgtct tggttaaccg gaaaaagaaa aatagtccct atcagtttga
     9061 aaaatctggc aatgatttac ttttcttgaa ggcacaaaca ggtcaagggg gcttttacgt
     9121 gaaaggaata aaagatgtca attgaattat ataaagtaag gcaggggaag atagtatctg
     9181 gggttcttgc cggcatagcg cataaattgg gctgggaatc ctgggtaaca cgcgcgatat
     9241 ttattgcagg tctcctctta ggcaaatcct tcctccttct gattgctctc tatattgcag
     9301 cagcttattt tttaccttat aaggaagata gggacgcgga acgctatgga acaggtccac
     9361 gtaagataaa agaagcggaa aagataaata agtcttggtt tgaataatgc aaaaaaactt
     9421 gagtttaaag gctcaagttt tttgtatg
//
LOCUS       DASFSW010000083         9392 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.083, whole genome shotgun sequence.
ACCESSION   DASFSW010000083 DASFSW010000000
VERSION     DASFSW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.083"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            39..773
                     /locus_tag="U1099_001451"
     CDS             39..773
                     /locus_tag="U1099_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867766.1"
                     /translation="MLEVKNVTGGYVNIPVLKDVSFTVEDGQLVGLIGLNGAGKSTTI
                     KEIIGLLTPYQGQIAIDGLTLLQGAENYRKKIGFIPETPSLYEELTLKEHLEVVAMAY
                     DLSWEQAWGRVEHLLKLFRLDEKLDWFPVNFSKGMKQKVMIICAFMVEPSLLIVDEPF
                     LGLDPVAISDLIALLGEEKSKGTSILMSTHVLDSAEKMCDSFVILHQGQVRATGNLEE
                     LQSAFGMDGASLNEIYLALTQEGVAG"
     gene            770..1810
                     /locus_tag="U1099_001452"
     CDS             770..1810
                     /locus_tag="U1099_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867767.1"
                     /translation="MKELFQQRRLDFLNRCSKYLRYVLNDHFVLVLMVLLGFLSLQYR
                     QLLLSFPENPLPIVFVVIAISLLVLVSGRIATYLEEADQIFLLTKEKDILQGLEVAAR
                     CTVLFWSSIQVVVQLVLLPIYLKLGLPVWMIVAGLLTLLVLKCLFVKRQLVAYQSQGV
                     LDWSKAIQDEQKRKQSILRFFALFTTVKGISTTVKPRSYLNGILRLVKSRQTWFYLYL
                     RAFLRSGDYLGLTIRLLLLAILSLFAIEESWLSIGLVLVFHYLLVFQLLGLYKHYDYQ
                     YITQLYPLEKDSKKIGLQNLLRVILYSLLLVELGFALLFSKENTMVVVLLLVGIFLHE
                     IYLPLKLKKLID"
     gene            1860..2654
                     /locus_tag="U1099_001453"
     CDS             1860..2654
                     /locus_tag="U1099_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEM4867768.1"
                     /translation="MQFDTSGLQLQSIAGNSGKAFKGLRSDGTPVFVKYEMPPIVSAL
                     AREQITPPVLSANREVGMGHRVEQEWLNGRTLERQDMTSKQIRQILVRMHYSRILLNQ
                     ALQMNYTYMEPQDLVYRWQEEAPTRLGQNSYLQSICQDLLNNLPRFRQEVATFVHGDL
                     HHSNWVETTSGLVYLTDWETACVTDRMLDVAYILTHYIPRQSWEEWLRAYGYKYNSTV
                     LNKVYWYGQLGYLNQIAKHVESYNVLAANKEIYALRQFRQSFYKEV"
     gene            2651..3289
                     /gene="trmB"
                     /locus_tag="U1099_001454"
     CDS             2651..3289
                     /gene="trmB"
                     /locus_tag="U1099_001454"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEM4867769.1"
                     /translation="MRVRNRKGASELLANNPQYVISNPEECKGKWAEIFGNNNPIHIE
                     VGSGKGRFVTGMAAQNPDINYIGIDIQMTVLSYALDRVLEAGLPNIKLLQVDGSSLTN
                     YFAPAEIDQLYLNFSDPWPKKRHEKRRLTYKSFLDTYKEILPEKGEIHFKTDNRGLFE
                     YSLASFSQYGMVLKQVWLDLHADGLEGNVMTEYEEKFSNKGQVIYRVEAQFQ"
     gene            3547..4017
                     /gene="rimP"
                     /locus_tag="U1099_001455"
     CDS             3547..4017
                     /gene="rimP"
                     /locus_tag="U1099_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEM4867770.1"
                     /translation="MSSIIELVTAAITPAIQTPYELVDVEYGKMGGDYVLSIFVDKEG
                     GISLQDTADLSEKISPILDTIKPDPFPDQYMLEVTSPGLERPLKTADAVEKAVGKYIH
                     VKLYQAIDKIKVFEGTLLSFDGTDLIMEYMDKTRKKEVTIPYQTVAKARLAVKL"
     gene            4090..5232
                     /gene="nusA"
                     /locus_tag="U1099_001456"
     CDS             4090..5232
                     /gene="nusA"
                     /locus_tag="U1099_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEM4867771.1"
                     /translation="MSKEMLEAFRILEEDMGINKADIIDAVTESLRSAYKRRYGQSES
                     AVIEFDEKKGDFHVFTVREVVDEVFDSRLEISLKDALAISSAYEMGDKIKFQEDPAEF
                     GRVAAQSAKQTIMEKMRKQKRAITFNTYKQHENEIMSGTVERFDNRFIYVNLGTIEAQ
                     LSKQDQIPGEVFQSHDRIEVYVYKVEDNGRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKITSKFHPARYDAKNDR
                     MIPTEENIDVIEWVADEAEFIYNALAPAEVDQVLFDTEDGKHATVVVPDDKLSLAIGR
                     RGQNVRLAAHLTGFRIDIKSASEYEAFEATQYATDEVVEEVAEEQE"
     gene            5249..5545
                     /locus_tag="U1099_001457"
     CDS             5249..5545
                     /locus_tag="U1099_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEM4867772.1"
                     /translation="MAKARKIPLRKSVVSGEIIDKRDLLRIVKNKEGQVFIDPTGKAN
                     GRGAYIKLDNDEASQAKKRRVFDRSFSMEVAEEFYDELIAYVDHKIKRRELGLE"
     gene            5502..5840
                     /locus_tag="U1099_001458"
     CDS             5502..5840
                     /locus_tag="U1099_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEM4867773.1"
                     /translation="MLIIRSRGENLDSNKRQKILNLLGLAQRAGRLVSGEDLVVEAIQ
                     KGQAKLVFLAEDAAGNLSKKVTDKSHTYQVEVVTVFSTLELSAAVGKARKVLAVTDAG
                     FTKKMRSIME"
     gene            5858..8680
                     /gene="infB"
                     /locus_tag="U1099_001459"
     CDS             5858..8680
                     /gene="infB"
                     /locus_tag="U1099_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEM4867774.1"
                     /translation="MSKKRLNEIARELGVSSKEVVAKAQELGFEVKSHASSVDEASAK
                     RLAESFGGQKSEATKVAAKVSKPEKVDETPKVETAKVEKAKETQPVVKEEVAASAVQS
                     VPHRPQSRNFKAEREARAKEQAAKRAQGQGKGGQAKSGQDRRDNRQQGQGRSNNERND
                     RRDNRRDQRPEERKDNRFGERRDNRANRRQDNRSGRSARFEQREAAKPAGPKIDFKAR
                     AAALKAEQNAEYARTSEERFRQAQEAKKQPKKAKEIKFEEPVVESKPFVKPAPVASVP
                     EQVAETTVDTRRKKQARPDKKRYFNSDEEDGPRKQQRNRNSQNQVRNQRTSNWNNNKK
                     NKKGKANQPAKPVTERKFHELPTEFEYTAGMTVAEIAKRIKREPAEIVKKLFLMGVMA
                     TQNQSLDGDTIELLMVDYGIEAKEKVEVDNADIERFFVEEGYLNEEEMTERPPVVTIM
                     GHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMR
                     ARGASVTDLTILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGE
                     LAEHGVISTAWGGESEFVEISAKFNQNIDELLETVLLVAEIQELKADPTVRAIGTVIE
                     AHLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKTAGPSTPISIT
                     GLNEAPMAGDHFAVYEDEKSARAAGEERAKRALLKQRQATQRVSLENLFDTLKAGEVK
                     SVNVIIKADVQGSVEALASSLQKIEVEGVRVNIVHSAVGAINESDITLAEASNALVIG
                     FNVRPTAEARSQAEADDVEVRLHSIIYKVIEEMEDAMKGMLDPEYEEKIIGEAIIRET
                     FKVSKVGTIGGFMVVRGKVTRDSSVRVIRDGVVVFDGKLVSLKRYKDDVKEVGNAQEG
                     GLMIENYNDLKVDDTIEAYIMEEIKK"
     gene            8836..9189
                     /gene="rbfA"
                     /locus_tag="U1099_001460"
     CDS             8836..9189
                     /gene="rbfA"
                     /locus_tag="U1099_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEM4867775.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMVGD
                     LSMAKVYYTIMSNLASDNQKAQTGLEKATGTIKRELGRKLTLYKIPDLVFEKDQSIEY
                     GNKIDQMLRALDQKD"
BASE COUNT         2910 a         1656 c         2244 g         2582 t
ORIGIN      
        1 ttgttataat aattatatca aattgtagga agaaatgtat gttagaagta aaaaatgtaa
       61 ctggaggata tgtcaatatc ccagttttga aagatgtatc ttttaccgtt gaggatggtc
      121 aattagttgg tctaattggt ttaaatggtg cagggaaatc aacgactatt aaggaaatta
      181 tcggtctttt gaccccctat caaggacaga ttgcaattga cggcctaact ttgctacaag
      241 gggctgaaaa ttatcggaag aagattgggt ttatccctga aacaccaagt ttgtatgaag
      301 aactaaccct caaagagcac ttggaagtgg tagcaatggc ctatgattta tcctgggagc
      361 aagcttgggg acgtgttgag cacttgttaa aactgtttcg cttggatgag aaattggact
      421 ggtttcctgt caatttttca aagggaatga agcagaaggt catgattatt tgtgctttta
      481 tggtggagcc gagtctattg attgttgatg agccttttct gggattagac cctgtggcta
      541 tctctgattt aatcgctctt ttgggggaag aaaaatctaa gggcacttca atcctcatgt
      601 ccactcacgt tcttgactca gctgagaaaa tgtgcgactc ttttgtgatt ttacatcagg
      661 gacaggtccg tgcaactgga aatttggagg agctacaaag cgcatttggt atggatggtg
      721 caagtctgaa tgaaatttat ctggccttga cgcaagaagg agtggctgga tgaaagaatt
      781 gtttcaacaa cgccgactag attttttaaa tcgttgttcc aagtatctgc gctatgttct
      841 gaatgaccat tttgttttgg ttctcatggt tttattaggc tttttgagtt tgcaataccg
      901 tcaattattg ttgtcgttcc ctgagaatcc gttgccgatt gtttttgtgg ttattgcaat
      961 cagtctactg gtactagtgt ccggaagaat tgcgacttat ctagaagaag ctgatcaaat
     1021 cttcctattg accaaggaaa aagacattct gcaaggactc gaggttgcag ccagatgcac
     1081 ggtcttgttt tggtcctcta tacaggtggt ggttcagctt gttttgttac caatctattt
     1141 gaaactgggt ttaccggtgt ggatgattgt agctggtctt cttactttgt tggttttaaa
     1201 atgtcttttt gtcaagcgcc aattagtggc ttatcagtca cagggagtat tggactggtc
     1261 taaggctatt caagatgaac aaaaaaggaa acagtctatt ttacgtttct ttgccttatt
     1321 tacgacggtt aaaggaatca gtacgactgt taaaccgaga agctatctca atggaatctt
     1381 gcgtcttgtc aagtcgagac agacttggtt ttatctctac ttacgggctt tcttgcgttc
     1441 aggagactac ttgggcttga cgatacgtct actgttgttg gcgattttgt ccctatttgc
     1501 cattgaagaa tcgtggttat ccatcggcct agttttagtc tttcattatt tgcttgtctt
     1561 tcagttgttg gggctttaca agcattatga ttatcaatat ataacacaac tgtatcctct
     1621 ggagaaagat tccaagaaaa ttggtcttca gaatttgctt agagtgattt tatatagtct
     1681 attactggtg gaattgggct ttgctctgct attttctaaa gagaatacga tggtagtcgt
     1741 cttacttctc gtggggattt tcttgcatga aatatatttg cctctaaaat taaaaaaatt
     1801 gattgactaa atagacgaaa attagtaaaa tagtaaggaa tttttaaagg aggaaaggca
     1861 tgcagtttga taccagtggg ctacaattac agtctattgc aggaaatagt ggcaaagcgt
     1921 ttaaaggcct acggtcagac ggaacgccag tctttgtgaa gtatgagatg cctccgatag
     1981 tgtctgctct tgcgcgtgag caaattacac cacccgtttt gtcagcaaat cgtgaagtag
     2041 gaatggggca tcgtgtagag caggagtggt tgaatggtcg taccttggag cgtcaagata
     2101 tgactagtaa acagattcgt caaatactgg tgagaatgca ctattctcgc attttgctca
     2161 atcaggcttt gcagatgaat tatacctata tggaacctca ggatttggtt tatcgttggc
     2221 aagaagaggc gccaactaga ttgggacaga attcttatct gcaatctatc tgtcaagatt
     2281 tattgaataa tctaccaaga ttccgtcaag aggtggcaac atttgttcac ggagatttgc
     2341 atcatagtaa ctgggtagaa acgacgtctg gtttggtgta tttgacagat tgggagaccg
     2401 cttgtgtaac ggatcggatg ttggatgttg cctatatttt gacgcattat attcctcgtc
     2461 agtcctggga agagtggctt cgggcttatg gctacaaata taacagcaca gtattaaata
     2521 aggtttattg gtatggccag cttggctatc taaaccaaat agctaagcat gtagaaagct
     2581 acaatgtttt ggctgcaaat aaagaaattt atgctctacg gcaattccgt cagagtttct
     2641 ataaggaagt atgagagtta gaaatcgtaa gggagcgagt gagcttctag caaacaatcc
     2701 ccagtatgtt atctccaatc cagaggagtg taaggggaaa tgggcagaga tttttggaaa
     2761 caataatcct atccacatag aagttggttc tggtaaaggt cgctttgtca ctggtatggc
     2821 tgcgcaaaat ccagatatta actatattgg tattgatatt cagatgaccg tgttaagcta
     2881 tgccttggat cgtgtgcttg aagctggctt accaaatata aaattattgc aggtggatgg
     2941 ttcgagtttg accaactatt ttgcaccggc agaaatagat cagctttatc tgaatttttc
     3001 agatccatgg cctaagaaac gtcatgagaa acgccgtttg acctacaagt cattcttgga
     3061 tacctacaag gaaatcctac ctgagaaggg agaaatccac ttcaaaacag ataatcgtgg
     3121 tctgtttgag tatagcctgg ctagtttttc tcaatatggc atggtcttga agcaagtttg
     3181 gctagatttg catgctgatg gtttggaagg caacgttatg acagagtatg aggagaaatt
     3241 ctcaaacaag ggtcaagtta tctatcgtgt ggaagcacag tttcaataat tcccttagtg
     3301 gaaaatgagg agaggtctaa cagagacctt tttcctttac aaatagggat agatatgata
     3361 gaataatgga agtgaataat gagaaaaggg gttggcgtca gtcaaccctt tccctgtaca
     3421 cggatttctg tatatagaat gaatgatagt acaagggagt ttactgtcaa aggaaataga
     3481 gagtcctgga tagtgcgaac tatttgagta atgtatttct agcgactgta aaaataggag
     3541 gtgtttatgt catcaattat tgaattggtc acagcagcaa ttacccccgc tattcaaact
     3601 ccttacgagt tggtagatgt ggaatatggt aaaatgggcg gtgactatgt cctatcgatt
     3661 tttgtcgata aggagggtgg aatttccctt caagatacgg cagatttatc cgaaaagatc
     3721 agtccgattt tggacactat caaaccagac ccatttccag accaatacat gctggaggta
     3781 acaagtccag gtttggaacg tcctttaaaa acggcagatg ctgttgagaa ggcggttgga
     3841 aagtacatcc atgtcaagct ctatcaggca attgataaaa tcaaggtttt tgaaggaacg
     3901 ttgctctcct ttgatggaac agacctgatt atggagtata tggataaaac tcgtaagaag
     3961 gaagtgacta ttccttacca gacggtggct aaggcccgtt tggctgttaa gttataatgc
     4021 tctttcatga gtattttaaa agatattaga ctttaagaaa ggaccttttg agcgcaaaag
     4081 gaaaacaaca tgagtaaaga aatgctagaa gccttccgta ttttagagga agacatgggt
     4141 attaacaaag cggatatcat tgatgcagtt acagagtcgc ttcgttcagc ttataaacgc
     4201 cgttatggtc aatcagaatc agcagtgatt gaatttgacg agaaaaaagg cgatttccat
     4261 gtatttactg ttcgtgaagt agtagatgaa gtatttgata gccgactaga aattagtcta
     4321 aaagatgcct tggctatttc atcagcttat gaaatgggtg acaaaattaa attccaagaa
     4381 gacccagcgg aatttggacg tgtagctgct caatcagcta aacagaccat catggaaaag
     4441 atgcgcaaac aaaagcgtgc cattaccttc aatacctaca agcaacatga aaatgagatt
     4501 atgtcaggta cggtagagcg ttttgataat cgctttatct atgtcaacct tggtacgatt
     4561 gaagcacaat tgtcaaaaca agaccaaatt cctggtgagg ttttccaatc tcatgatcgc
     4621 atcgaagtct atgtctacaa ggtagaagac aatggtcgtg gtgttaacgt atttgtcagc
     4681 cgtagccatc cagaaatgat caaacgtctt atggaacagg aaattccaga agtatacgat
     4741 ggaacagttg aaatcatgag tgttgcccgt gaagctggag atcgtacgaa agtggctgtt
     4801 cgtagccata atccaaacgt agatgccatc ggtacgatcg ttggtcgtgg tggttcaaat
     4861 atcaagaaaa tcaccagcaa gttccaccca gctcgttatg atgctaagaa tgaccgtatg
     4921 attcctactg aggaaaatat tgacgttatc gagtgggtag cagatgaagc ggaatttatc
     4981 tacaatgctc tcgctcctgc cgaagttgac caagtattgt ttgacacgga agatggaaaa
     5041 catgctacag tcgttgtacc agatgataaa ttgtcattgg caattggtcg tcgcggtcaa
     5101 aacgttcgtt tggcagcaca tttgacagga ttccgtatcg acatcaagtc agcatcggag
     5161 tatgaagctt ttgaagcaac tcaatatgca acagacgaag ttgtagaaga agtcgcagaa
     5221 gaacaagaat aaggcaaaag gagcaaccat ggcaaaagct agaaaaatac ccttgcgcaa
     5281 gtctgtggtg tccggtgaaa tcatcgacaa acgggatttg ctccgtattg taaaaaataa
     5341 agaaggtcaa gtatttatcg atccaacagg caaggcaaat ggtcgtggag cttatatcaa
     5401 gcttgacaat gatgaggcaa gccaagctaa gaaacgtcgt gtcttcgatc gttcctttag
     5461 tatggaagtg gccgaagaat tttacgatga attaattgcc tatgttgatc ataagatcaa
     5521 gaggagagaa cttggactcg aataagagac agaaaatctt aaatttatta ggtttggccc
     5581 agcgagctgg tcgcctggtt tcgggtgagg acctggttgt tgaagctatt caaaaagggc
     5641 aggcaaagtt ggtgttttta gccgaagatg ctgctggaaa tttaagtaaa aaagtaacag
     5701 ataaaagtca tacctatcaa gtagaagttg taacagtgtt ttcaacactg gaattgagtg
     5761 cagctgtcgg caaggctaga aaagttcttg cagtgacaga tgctggattt acaaagaaga
     5821 tgaggtctat tatggaatag aacaggagga caaggcattg tctaagaaga gattgaatga
     5881 aatagcccgt gaattaggcg taagcagtaa ggaagttgtt gctaaggctc aagaattggg
     5941 ttttgaagtc aaaagtcatg cttcaagcgt agatgaagca agtgctaaac gccttgcaga
     6001 aagctttgga ggacaaaaat cagaggcaac taaagttgcg gcaaaagtta gcaagcctga
     6061 gaaggttgat gaaactccaa aagtggaaac agctaaggta gaaaaagcta aggaaacaca
     6121 gccagtagta aaagaagaag ttgcggcaag cgcagtacaa tcagttccac atcgtccaca
     6181 gagtcgaaac tttaaggcag agcgtgaagc acgggccaaa gagcaggctg ctaagcgagc
     6241 tcagggtcaa ggaaaaggtg gtcaagctaa atctggtcag gaccgtcgtg acaaccgtca
     6301 acaaggtcag ggacgttcaa acaatgagcg aaatgatcgc cgcgacaatc gtcgtgacca
     6361 gcgtccagaa gagcggaaag acaaccgatt tggtgaacgc cgcgataatc gtgccaaccg
     6421 tcgtcaggac aatcgttcag gtcggtcagc tcgctttgaa caaagagagg ctgcaaaacc
     6481 agcaggacct aaaattgact tcaaagcgcg tgctgctgct ttgaaagcag aacaaaatgc
     6541 ggagtatgct cgtaccagtg aagaacgttt tcgtcaggct caggaagcta aaaagcaacc
     6601 taagaaggca aaagaaatta agtttgaaga accggttgtg gaaagcaaac catttgtgaa
     6661 gcctgcacct gttgcatcag taccagagca agttgcagaa actactgtag atacccgtcg
     6721 taagaaacaa gctcgaccag ataaaaaacg ttactttaat agtgacgaag aagatggtcc
     6781 acggaaacaa caaagaaatc gaaatagtca aaatcaagtg agaaatcaaa gaacaagtaa
     6841 ctggaataac aacaagaaaa ataaaaaagg caaggctaac caacctgcca aacctgtcac
     6901 agaacgcaag ttccacgaat tgccaactga atttgaatac actgctggca tgaccgtcgc
     6961 agaaattgca aaacgaatca aacgtgaacc tgctgaaatt gtgaaaaaac tcttccttat
     7021 gggagttatg gcgactcaaa accaatcttt ggatggagat actatcgaac tcctcatggt
     7081 tgattatggt attgaagcga aagaaaaggt agaggtcgat aacgcagata ttgagcgttt
     7141 cttcgtagaa gagggctacc tcaatgaaga agagatgaca gaacgcccac ctgttgtgac
     7201 tatcatggga cacgttgacc atggtaaaac aaccttgttg gataccctac gtaattctcg
     7261 tgttgctact ggggaagctg gaggtatcac acagcatatc ggtgcctacc aaattgagga
     7321 agctggtaag aaaattacct tcttggatac tccaggacac gcggccttta cttctatgcg
     7381 tgcacgtggt gcatctgtta ccgacttgac catcctagtc gttgctgcag atgatggtgt
     7441 catgccacaa acgattgagg ctatcaacca ctcaaaagca gctaatgtac caatcattgt
     7501 agctatcaac aagattgaca agccaggtgc aaacccagag cgtgtcatcg gtgaattggc
     7561 tgagcacggt gttatctcaa ccgcttgggg tggagaatct gaatttgtag aaatctcagc
     7621 taagtttaac caaaacattg atgaattgtt ggaaactgtt cttttggttg ctgaaatcca
     7681 agaactgaag gcagacccaa ctgttcgtgc gattggtaca gttatcgaag cgcatttgga
     7741 taaagggaaa ggtgcggttg caaccctcct cgttcaacaa gggacactta atgttcaaga
     7801 cccaatcgtt gtcggaaata ccttcggacg tgttcgtgcc atgacaaacg accttggtcg
     7861 tcgtgttaaa actgctggtc catctacacc gatatctatt actggtttga acgaagcgcc
     7921 tatggcaggc gatcattttg cagtttacga ggatgagaaa tcagcccgtg cagcaggtga
     7981 agaacgtgct aaacgtgctc ttcttaaaca gcgtcaagca acgcaacgag ttagtcttga
     8041 aaacctcttt gataccctta aagcaggtga ggttaagtca gttaatgtga ttatcaaggc
     8101 cgacgtacaa ggttcagttg aagctcttgc atcatcgctt caaaagattg aagtagaggg
     8161 agtacgtgtc aatatcgtcc actctgcagt tggtgctatc aatgaatcag atattactct
     8221 tgcggaagct tcaaatgctc ttgtgattgg tttcaacgtt cgtccaacag ctgaagcgcg
     8281 tagccaagct gaagcagatg atgttgaagt acgtcttcac agcatcattt acaaggttat
     8341 cgaagagatg gaagatgcca tgaaaggtat gcttgatcca gaatatgaag aaaaaatcat
     8401 tggtgaggca atcattcgtg aaaccttcaa ggtatccaaa gttggaacga tcggtggttt
     8461 catggtcgta cgtggtaagg ttacccgtga ttcaagtgtt cgagttatcc gtgatggtgt
     8521 tgtagtattt gatggtaaac tagttagttt gaaacgctat aaagatgacg tgaaagaagt
     8581 tggtaatgcc caagagggtg gtttgatgat tgaaaactac aacgacttga aagttgatga
     8641 taccattgaa gcttacatca tggaagaaat caaaaaataa tattttctca ttgtcaaaat
     8701 catagacaag tgattttgtc gaggaagtga caatttaata gattttagta gattttaaag
     8761 caatagtttt taatctactt ggattattca agctggggaa actcagcttt tctatcatct
     8821 aagaaaggaa agaatatggc gaatcatttt cgtacagacc gtgtcgggat ggaaatcaag
     8881 cgcgaagtca atgagatttt gcaaaagaaa gttcgtgatc cacgtgtgca gggagtgacc
     8941 attacagatg ttcagatggt tggcgatttg tcaatggcca aggtttacta cacaatcatg
     9001 agtaatttgg catccgataa tcaaaaagca caaacgggtc ttgaaaaagc gacgggaacc
     9061 attaagagag aacttggtcg taagttaact ctttataaaa ttccggattt ggtctttgaa
     9121 aaagaccaat caattgagta cggcaataaa attgaccaaa tgttgcgtgc tcttgatcag
     9181 aaagattaac aacaaaagtc ggatggaaac attcggcttt ttctggctcg gatgctttgt
     9241 ccatatatgg tggcgtttac aagccctcgg aaagatgtta tactaatact caatgaaaat
     9301 caaaagtaga ctaggaaacg aagtcgaaga tagaactctg tcactatctt atttcaaggt
     9361 aacaggctga aaacctccac tggagctttt ca
//
LOCUS       DASFSW010000084         9085 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.084, whole genome shotgun sequence.
ACCESSION   DASFSW010000084 DASFSW010000000
VERSION     DASFSW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.084"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            106..369
                     /locus_tag="U1099_001461"
     CDS             106..369
                     /locus_tag="U1099_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEM4867776.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLNYKEKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAITETMEKEGLA"
     gene            374..2107
                     /gene="ptsP"
                     /locus_tag="U1099_001462"
     CDS             374..2107
                     /gene="ptsP"
                     /locus_tag="U1099_001462"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEM4867777.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DAALEASQNELSVIRENAVASLGEEAAAVFDAHLMVLADPEMIGQIKETIRAKKTNAE
                     TGLKEVTDMFIAIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVRLPNPATIDEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     VLAVNGITGDVVINPSEEVIAEFKVAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPHEMNPFLGFRALRISISETGNQMFRTQLRALLRSSVHGKLRI
                     MFPMVALLTEFRAAKAILEEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYAHRALTECATAEEV
                     LELQKEYVDFD"
     gene            complement(2189..3676)
                     /locus_tag="U1099_001463"
     CDS             complement(2189..3676)
                     /locus_tag="U1099_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEM4867778.1"
                     /translation="MKKIAFDSTKYLNLQRDHILERIAQFEGKLYMEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKDQVEIVIAINASNIEHSKARGDLGISYDQEVFRLIDTF
                     NDIDIYVGSVVITQYRNQPAADAFRKQLEKHGIKSYLHYPIKGYPSDIDHIISPEGMG
                     KNDYIETSRNLVVVTAPGPGSGKLATCISQLYHDQLHGVTSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDLNMIDPFHLQTYGKTAVNYNRDIEVFPVLNRTFERILNKSPYA
                     SPTDMGVNMVGYSIVDEEAAIEASKQEIIRRYYQTLVDFKAERVSEQAVKKIELLMNE
                     VGVTPADRKVVIAAREKAELTTSPALAIQLPTGEIVTGKTSDLLKPTATVLLNAIKQI
                     ANIDDETLLIEPNYIRPIQELKADYLDKTNTRLDASEILNALAITAQDSPLAARAMKE
                     LGQLNGSEAHSTVILSDEDKSVLRKLGINLTFDPIYQHNKFYQKN"
     gene            3948..5216
                     /locus_tag="U1099_001464"
     CDS             3948..5216
                     /locus_tag="U1099_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM4867779.1"
                     /translation="MKKLIHNKLFLSSFVADLISNFGDTLYYLALMNHVLLLADTKFA
                     LAMITASETLPILAGLFIGIWADKTKNKLDTILATLVVRVGLYAFVGFLMGFAPALWV
                     VAVVCVINFLSDLAGQYENGLYMPVSLRVVAAEDRETAMAFKMTVKSLLQIAFQASGA
                     ILIGFMSYQNLAFFNAGTFLVSLAIMATLRPALAKLLKENPIQEAEQTETEAGLIKGM
                     RNSLKESYQAVQNILVLKASIVTIASLNAIFTALSPLVILNMKEFSDFVIVNAGTTVA
                     LISIFFSVGSILGSSIGMALFKNVSLINLLKFSTLMPVLIFSGFFLHNIYVVLTVIFV
                     TAMTLGVFNPKMNALVLNELPEDKLATVGGGIDAFCQIGMVAGQALVALMVTILSVTS
                     ISLIFLLLSVGLVSYTILVGCKPKNEVAQV"
     gene            5228..6268
                     /locus_tag="U1099_001465"
     CDS             5228..6268
                     /locus_tag="U1099_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEM4867780.1"
                     /translation="MKLDYRDYRSEIVEKLFIPLIVKEREEPFEADLSQKEKDILKDA
                     LDIRNEVENKLADFRQEVNEVFVWGHIFTILHTLYFYLLDQGQDPKTVEEACQLILTL
                     SQEEVEDAMRTMLASENDGHREKDLSLMELLEKTDKKPAEKWYWSLALRNPLETVERS
                     VDLLNKLLPIYQPYFEGARAEREAFARDFDIEQLYRESKQLAMTSLDSLGVETAQFFV
                     LSPWNYWFAYYGNEQFDYMKVALLASCRIDQIMLSNDELDLDDLTTALKVISDSTRYQ
                     VLVELTKPHAKSKDIAERMAITGAAVSFHTQKLINGDLLLFNTKNSDVKYSVNRELLQ
                     EVIAKLTEDFDL"
     gene            complement(6405..7394)
                     /locus_tag="U1099_001466"
     CDS             complement(6405..7394)
                     /locus_tag="U1099_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867781.1"
                     /translation="MQNKTKQLNTSLLSLIQFIGAILVVALHCRRLFEADQLHFIQKS
                     IFSRMVVPYFMVTASFFLRRNYPSLSKQYLIRYSKQYLTWSILYLPFYLIYLTLQEIP
                     FLYYPLALLVGLTYTGTSYQLWYMPAFFLGLILVHLSLEKWGWRITACLACLLYLLGS
                     VETYSAYLAESIFLTAFDNYKTFFFTSRNGLFYAPIFVLIGFYLANKLNHPIFQHRQK
                     TKLLVCTALLAFEATVIYLNQGYDKNFLFSLIPFTAYLVAWTLTTDLFRKKKFQFLKE
                     YASYYYFIHVIPVEISFFFLETSSLTKIQQGWLVFLITIITCQLLSWLIINTQ"
     gene            7583..8269
                     /locus_tag="U1099_001467"
     CDS             7583..8269
                     /locus_tag="U1099_001467"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEM4867782.1"
                     /translation="MKDLHAKLLADFGIDFSDLNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLMISKYLYQKYPDRPEGEMSKLRSTFVREESLAGFSRACGFDRFLRL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKGEKTVEEFIHKVMIPRLEKGNFERVTD
                     YKTALQEILQVNGEIAISYQVIAESGPAHDKTFEVEVSADRRVIGRGRGRSKKLAEQA
                     AAKNAVEARG"
     gene            8272..>9085
                     /locus_tag="U1099_001468"
     CDS             8272..>9085
                     /locus_tag="U1099_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4867783.1"
                     /translation="MYLKSIEMQGFKSFADKTKVVFDRGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFSGTESRKALNYASVVVTLDNSTGFIANKQKEIKVER
                     HIYRSGDSEYLIDGQKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETESKLAQAQGNLDRLDDIIYELDNQVKPLEKQAQTAKKFLEL
                     DGQRKELYLDVLVAQLLLGKEKLSEKEAELESVKTELTSYYKQRSELEQENQN"
BASE COUNT         2567 a         1681 c         2208 g         2629 t
ORIGIN      
        1 aaggtaaaaa aatcttgaaa atgatttcaa aaagtggtat aatgtccttt gtaagggtta
       61 tcacaaataa ctcacaattt tgtaaaaata aaggagaatc caaatatggc ttcaaaagac
      121 ttccacattg tggcagaaac aggtatccac gcacgtccag caactttgct tgtgcaaaca
      181 gctagtaaat ttgcttcaga catcactttg aactacaaag aaaagtctgt taaccttaaa
      241 tctatcatgg gtgttatgag tcttggtgtt ggtcaaggcg ctgacgtaac aatctctgct
      301 gaaggtgctg atgctgacga cgcaatcgca gctatcactg aaacaatgga aaaagaagga
      361 ttggcataag aatatgacag aaatgcttaa aggaatcgca gcatcagatg gtgttgctgt
      421 tgctaaggca tatctactcg ttcagccaga tttgtccttc gaaactgtta cagttgaaga
      481 tacaaatgca gaggaagctc gtttggatgc tgctctagaa gcatctcaaa acgagctttc
      541 tgttattcgt gagaacgcag tagctagcct aggtgaagag gccgcagccg tatttgatgc
      601 acacttgatg gttcttgctg acccagaaat gattggtcaa atcaaggaaa caattcgtgc
      661 gaagaaaaca aacgctgaga ctggtttgaa agaagttacg gatatgttta tcgctatctt
      721 tgaaggtatg gaagataacc catacatgca agagcgtgcc gcagatattc gtgacgtagc
      781 caaacgtgta ttggcacatt tgcttggtgt tcgcttgcca aacccagcaa ctatcgacga
      841 agagtcaatc gttatcgcac atgacttgac accttcagat acagcgcaat taaacaaaca
      901 gtttgtaaaa gctttcgtaa cgaacattgg tggtcgtaca agtcactcag ctatcatggc
      961 tcgtacactt gaaattgctg ctgttttggg tacaaataac attactgaaa tcgttaaaga
     1021 cggcgatgta ttggctgtta acggtatcac aggtgatgta gtgattaacc cttctgaaga
     1081 agtgattgca gaatttaaag tagctggtga agcctatgct aaacaaaaag ctgaatgggc
     1141 tcttttgaaa gatgctcaaa cagttacagc tgacggcaaa cactttgaat tggcagcaaa
     1201 tatcggtaca ccaaaagacg ttgaaggtgt aaatgataac ggtgcagaag ctgttggtct
     1261 ttaccgtaca gaattcttgt acatggattc acaagacttc ccaactgaag acgagcaata
     1321 cgaagcttac aaggctgttc ttgaaggcat gaatggtaag ccagtcgtag ttcgtacaat
     1381 ggatattggt ggtgataagg aacttcctta cttcgatctt ccacacgaaa tgaacccatt
     1441 cctaggtttc cgcgcccttc gtatctctat ctctgagact ggtaaccaaa tgttccgcac
     1501 tcagttgcgt gcccttcttc gttcatcagt acatggtaaa cttcgtatca tgttcccaat
     1561 ggttgccttg ttgactgaat tccgtgcagc aaaagcaatc cttgaagaag aaaaagcaaa
     1621 cttgttggct gaaggtgtag cagttgcgga tgatatccaa gttggtatca tgattgaaat
     1681 ccctgcagca gcaatgcttg cagaccaatt tgccaaagaa gttgacttct tctcaatcgg
     1741 tacgaacgac cttatccagt atacaatggc tgcagaccgt atgaatgaac aagtttcata
     1801 cctctaccaa ccatataacc catcaatcct tcgcttgatc aacaatgtta tcaaggctgc
     1861 acacgcagaa ggtaaatggg ctggtatgtg tggtgagatg gctggtgatc aacaagctgt
     1921 tccacttctt gtaggtatgg gcttggatga gttctctatg agtgcaacat ctgtacttcg
     1981 tacacgtagc cttatgaaga aacttgatac agctaagatg gaagaatacg ctcaccgtgc
     2041 cttgacagaa tgtgcaacgg cagaagaagt tcttgaactt caaaaagagt atgttgattt
     2101 cgattaatcg ttgaaaaaag cagggaattg aatccttgct tttttactgt tcacaaaaag
     2161 acttgtcgga gcaagtcttt ttgtgtgctt agtttttctg ataaaactta ttgtgttggt
     2221 agatagggtc aaaggtaaga ttgatgccta atttacgaag gacactcttg tcttcatcgg
     2281 ataggataac agtcgagtgg gcttcgctac catttaattg gcctaattct ttcatggcac
     2341 gtgcagcaag agggctatct tgtgcagtga tggcaagggc gttaagaatt tcgcttgcat
     2401 cgagtcgtgt atttgtctta tccagataat ctgccttcaa ttcttgaatt ggacggatgt
     2461 agtttggttc gatgagaagt gtttcatcat cgatgttagc aatttgcttg atggcgttga
     2521 gaaggacagt tgcagttggc ttgaggaggt cggatgtctt acctgttaca atctctccag
     2581 ttggcaactg aatagcaagg gcagggctag ttgttagctc tgctttttcg cgcgcagcga
     2641 taacaacctt acgatctgct ggcgtcacac cgacttcatt catgaggagt tcaatcttct
     2701 taactgcttg ctcgctcacc cgctctgcct tgaaatcaac caaggtctgg tagtagcggc
     2761 ggatgatttc ttgcttggat gcttcaatcg cagcctcttc gtccacgatg gaatagccca
     2821 ccatgttaac gcccatgtct gtcggtgagg cgtatggtga tttgttcaaa atacgttcaa
     2881 aggtacggtt gaggacaggg aagacctcga tatcacggtt gtagttgacc gcagttttgc
     2941 cgtaagtttg caggtggaag gggtcgatca tgttgaggtc gtccaggtca gcagttgctg
     3001 cttcataggc caagttaacg ggatggtgga gtggtaagtt ccacactggg aaggtttcaa
     3061 acttagcata gcctgatgtt actccatgca attggtcgtg gtaaagttgg gagatacagg
     3121 tcgccaattt acctgaacca ggtccaggtg cggtgacaac gacgagattg cgactagttt
     3181 caatgtagtc atttttgccc ataccttctg gtgaaatgat gtggtcgata tcagaaggat
     3241 agcccttgat tgggtagtgg agataggact tgatgccgtg tttttccaac tgcttgcgga
     3301 aggcgtctgc ggctggttga ttgcggtact gggtaatgac cacagaacca acataaatat
     3361 caatatcatt gaaggtgtca atcaagcgga agacttcttg gtcataagaa atacccaagt
     3421 caccacgtgc cttagaatgt tcgatgttgc ttgcattgat ggcgatgacg atttctactt
     3481 ggtcttttaa ctcttggagg agtttgattt tgttatctgg ttcatagcca ggtaaaacac
     3541 gggctgcgtg gaaatcttcc agcatttttc cgccaaattc catatagagc ttgccttcaa
     3601 actgagcaat tcgttctaaa atatggtcac gttgcaaatt caagtattta gttgaatcaa
     3661 atgcgatttt cttcatcaaa taagtcctcc aaatgtgtat taatctctgg gaaaaacaaa
     3721 aatagctccc taaaactagg gagccttttc tccgacctct caaaagaggt gcccgaacaa
     3781 tactataaca aaccgtgcta atcttgtcaa gaaaaagtca gacagaagaa aagttaaaca
     3841 agtgtttaaa aaatatttaa tttttataaa gttaaatatt gactttatta aaataaagtg
     3901 taaaataata ttaaacaaat atttaatcga tgtttaagga gatgtttatg aaaaagttga
     3961 ttcacaataa attatttcta tctagctttg tagcagactt gatttctaac tttggagata
     4021 ctctttacta tctggctttg atgaaccatg ttttactctt agcagatacc aagtttgccc
     4081 tggctatgat taccgcatct gaaaccttgc cgattttggc tggtctcttt atcgggattt
     4141 gggcagacaa gaccaaaaac aaattggata ccattcttgc aactctggtg gttcgtgtcg
     4201 gtctctatgc ctttgttggc ttcttgatgg gatttgcacc agccctctgg gttgtggcgg
     4261 tggtctgcgt catcaacttc ctgtctgacc ttgctggtca atacgaaaac ggtctctata
     4321 tgccagtcag cttgcgggtt gtggctgctg aagaccgtga aacagctatg gcttttaaga
     4381 tgacggtgaa aagtcttttg caaattgcct tccaagctag cggtgctatc ctcatcggat
     4441 tcatgtctta ccagaatctc gccttcttca atgcagggac cttcttggtc agtcttgcca
     4501 tcatggcaac cctccgtcca gctcttgcca agctactgaa ggaaaatcct atccaagaag
     4561 ctgagcaaac agaaacagaa gcaggactta tcaagggtat gcgtaacagt ctcaaagaat
     4621 cctaccaagc tgttcagaat attcttgttc tcaaagcgtc aattgtgacc attgccagcc
     4681 tgaatgccat ttttacagca ctttcaccat tagttattct caatatgaag gagttctcag
     4741 actttgtgat tgtcaatgca ggaacaaccg ttgctttgat ttccattttc ttctcagttg
     4801 gtagtatctt ggggtcaagt atcggtatgg ctttgttcaa aaatgttagc ttgatcaatc
     4861 tcctcaaatt ctctacattg atgccagtct tgattttctc gggtttcttt ctccacaata
     4921 tttatgtggt attgacggtt atttttgtga cagctatgac tttgggtgtc ttcaatccga
     4981 aaatgaatgc cttggtctta aatgaactgc ctgaagataa attggcgaca gtcggtggag
     5041 ggattgatgc tttctgccag attggtatgg tagcaggtca agccttggta gcccttatgg
     5101 taacaatctt atctgtcaca agtatttccc tcatcttcct cttgctttct gtgggactag
     5161 taagctatac tattcttgtg ggttgtaaac ctaaaaatga agtagctcaa gtttagaaag
     5221 tatgcgtatg aaattagatt atcgagatta ccgcagtgaa attgtggaaa aactgttcat
     5281 tcccttgatt gtcaaggagc gtgaggagcc gtttgaagcg gatttgtcac agaaggagaa
     5341 agacattctc aaagatgcct tggacattcg caatgaagta gaaaacaagc tggcggattt
     5401 tcgtcaggaa gtcaatgagg tctttgtttg gggccatatc tttacaatcc tgcacacctt
     5461 gtatttctac ctcttggatc aaggacagga tccaaagaca gtcgaggagg cttgtcagct
     5521 gattttgacc ttatcgcaag aagaggtaga agatgctatg cggaccatgc tagcgtctga
     5581 aaatgacggt catcgtgaaa aggacctcag cctcatggaa ctcttggaaa agacagataa
     5641 aaagccagca gaaaagtggt attggtcact agcccttcgc aatcccttgg aaactgttga
     5701 gcggtctgtc gatctcctga acaagctact cccaatctat cagccgtatt ttgaaggggc
     5761 aagagcagag cgggaagcct ttgctagaga ctttgacatt gaacaactct acagagaatc
     5821 caagcagtta gccatgacca gcttggatag cttgggtgta gaaactgccc aattcttcgt
     5881 tctcagtcca tggaattatt ggtttgctta ttatggcaat gaacaatttg actatatgaa
     5941 agttgctctt ttagcttcct gccgaattga tcagattatg ctgtcaaatg acgagttgga
     6001 tctggatgat ttgacaacgg ctcttaaagt catcagcgat agtacgcgtt atcaggtctt
     6061 ggtggagctg accaagcctc atgccaagag caaggacatt gcagaacgca tggctattac
     6121 aggggcggcc gtatccttcc acacccaaaa gctaattaac ggtgacttac ttctcttcaa
     6181 tacaaaaaat agcgatgtca aatacagtgt caatcgtgaa cttctccaag aggtcattgc
     6241 caaattgaca gaggattttg atttgtaaaa aaagccttgt aagttagatc ttacaaggtt
     6301 cttttgtgct tatactcttc gaaaatcgaa accagacgtt gttgacttga tttgatgaac
     6361 ttcagttcta tccgcatctg cgtcgcctag tctgtttttg attttcattg agtattaatg
     6421 attaaccagc tgagtagttg gcaagtgatg atggtgataa ggaagactaa ccacccttgc
     6481 tgtattttgg tcagggaact agtttctagg aagaaaaagc tgatttcgac tgggataaca
     6541 tggataaaat aataatagct ggcgtattct tttagaaatt ggaatttttt cttgcggaag
     6601 aggtctgtgg ttaaggtcca agcgactaga taggctgtga acggaatcag gctaaataga
     6661 aagttcttgt cgtagccctg attgagatag atgacagtgg cttcaaaagc taaaagggct
     6721 gtgcagacaa gtagtttggt tttttgtcgg tgttggaaaa tggggtgatt gagtttgttg
     6781 gctagataaa agcctatcag gacaaaaatc ggtgcgtaga agaggccgtt gcgactggtg
     6841 aaaaagaagg ttttgtagtt gtcaaatgcg gtcaggaaaa tcgattctgc taggtaggcg
     6901 gagtaggttt cgactgagcc tagaagatag agaaggcagg ctagacaagc tgtgatgcgc
     6961 cagccccatt tctccaagga aagatggacg aggataagac ctagaaagaa agcgggcatg
     7021 taccagagtt ggtagctggt gccggtatag gtcagtccga ctaggagtgc gaggggatag
     7081 tagaggaagg ggatttcttg gagtgttagg tagatgaggt agaagggcag gtagaggatg
     7141 gaccaggtca ggtattgttt gctgtagcgg atgaggtatt gtttggagag gctggggtag
     7201 ttgcgtcgga ggaagaagct ggcggtgacc atgaagtagg gaacgaccat gcggctaaag
     7261 attgattttt ggatgaagtg gagctggtct gcttcaaata gtcttcgaca gtgaagggca
     7321 acgacgagga tggctccgat gaactggatg agggagagca gtgatgtatt gagttgtttt
     7381 gtcttatttt gcatgctaac gttcttcttt tttcttctat tttatcgaaa tctgatgaat
     7441 atgtaaatgt ttgcaaaaag atgtggagag gtgctggctg aaacttagtt gacaagagaa
     7501 gtccagtgga aaatatggta taatgttagg acggagtaaa tgtaaaccat ctatttcgtt
     7561 taagttagaa aggagcatat agatgaaaga tttacatgca aaattattgg ctgattttgg
     7621 gattgatttt tcggatttga atttattaga aacggctttt acccatactt cttatgccaa
     7681 tgagcatcgc cttctaaaaa tttcacataa tgagcgcttg gaatttttag gagacgctgt
     7741 tctccaatta atgatttcca aatatctcta tcaaaaatat cctgatagac ccgaaggtga
     7801 gatgtccaag ttgcgttcga cctttgtacg tgaggagtct ttggctggtt tttcacgtgc
     7861 ttgtggtttt gatcggtttc ttcgtcttgg taagggggaa gagaagtctg gagggcgaaa
     7921 tcgcgatact attttaggag atgctttcga ggcgttctta ggagctctgc tattggacaa
     7981 aggggaaaag accgtggagg aatttatcca taaggtcatg attcctcgtt tagaaaaagg
     8041 gaattttgag cgcgtgaccg actataagac tgctttgcag gaaattttgc aggtcaatgg
     8101 tgagattgct atttcgtatc aagtaatagc tgagtctgga cctgcccatg ataagacctt
     8161 tgaagtggag gtatctgctg atcggcgtgt gattggtcgt ggtcgtggtc gctctaagaa
     8221 attagctgaa caagccgccg ccaaaaatgc tgtggaggct agaggatagg tatgtatcta
     8281 aaatcaattg aaatgcaggg ctttaagtcc tttgcggata agactaaggt agtctttgat
     8341 cgtggagtga ctgctgttgt agggccgaat ggttctggca agtccaatat tacagaaagt
     8401 ctacgttggg ccttgggaga gtcgtctgct aaaagcctac gtggaggcaa gatgccagac
     8461 gtcatctttt cgggaacaga aagccgcaag gctcttaact atgcctcggt tgtcgtgacc
     8521 ttggataata gcacaggttt tatcgccaat aaacagaaag aaatcaaggt agaacgccac
     8581 atttatcgat ctggtgatag tgagtacttg attgatgggc aaaaggttcg tctacgggat
     8641 attcatgatc ttttcatgga tacgggcttg ggacgtgatt ctttctctat catttcacaa
     8701 gggcgggttg aagcaatttt caattccaag cctgaggaac gccgtgctat ttttgaagag
     8761 gcggcagggg tcttaaaata caagacgcgt aaaaaagaaa cagaaagtaa gttggcacag
     8821 gcccagggca acttggaccg cttggatgat attatctatg aattggataa tcaggtcaaa
     8881 ccattagaga aacaggcaca aactgctaag aaatttctag aattagatgg tcaacgtaag
     8941 gaattatact tagatgtttt ggtggctcag ttgttgcttg gtaaggaaaa gttatcggaa
     9001 aaagaagccg aactagagtc tgtcaaaact gaattgacta gctactacaa acagcgttct
     9061 gagctggagc aagaaaatca gaact
//
LOCUS       DASFSW010000085         8873 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.085, whole genome shotgun sequence.
ACCESSION   DASFSW010000085 DASFSW010000000
VERSION     DASFSW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.085"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(307..555)
                     /locus_tag="U1099_001469"
     CDS             complement(307..555)
                     /locus_tag="U1099_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEM4867784.1"
                     /translation="MFYLILAIMLVLFYIFIAPRNIKGTMNLIAAVFLLVALAIALVL
                     GFLRIMQFPKEIWVGLLMILIGFWAMRDIYQLNDDTKF"
     gene            complement(573..2420)
                     /gene="typA"
                     /locus_tag="U1099_001470"
     CDS             complement(573..2420)
                     /gene="typA"
                     /locus_tag="U1099_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEM4867785.1"
                     /translation="MTKLREDIRNVAIIAHVDHGKTTLVDELLKQSQTLDERTQLDER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLTPIVVVNKIDKPSARPEEVVDEVLELFIELGADDDQ
                     LEFPVVYASAINGTSSLSDNPADQEETMAPIFDTIIEHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAVEALPVLRIDEPTLQMTFLVNNSPFAGREGKWVTSR
                     KIEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKMEPFERVQIDTPEEYQGSVIQSLSERKGDMLDMVATGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIDQGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITTAKQMTNVRSATKDQTSVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAERDKAAKKKKLATEE"
     gene            complement(2553..3545)
                     /locus_tag="U1099_001471"
     CDS             complement(2553..3545)
                     /locus_tag="U1099_001471"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEM4867786.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEIVEVQGPILSRVGDGM
                     QDNLSGVENAVTVNVKLIPDATYEVVHSLAKWKRHTLARFGFHEGEGLFVHMKALRPD
                     EDSLDPIHSVYVDQWDWEKVIPDGRRNLAYLKETVEQIYKAIRLTELAVEARYDIESV
                     LPKKITFIHTEDLVKNFPDLTPKERENVVAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTSPSEAGYKGLNGDILVWNEALGSAFELSSMGIRVDEEALKRQVAITGDEDRLEF
                     DWHRALLNGLFPLSIGGGIGQSRLAMFLLRKKHIGEVQSSVWPQEVRDNFENIL"
     gene            complement(3630..4679)
                     /locus_tag="U1099_001472"
     CDS             complement(3630..4679)
                     /locus_tag="U1099_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEM4867787.1"
                     /translation="MSRQIAHLPSLIPFGFILADNRYTYREVFMEGQFEAVVEVDEAG
                     QLSSYVWDCEMEEVYTAHLVTAPAGAFVGQVREAYQSILARVEEVCCIALPFSKDQSN
                     RIAQLIKEKWGDLPDYPFAKLPTYGAFRHPSNNKWYALVSQIPRDKLDGSGSKEEVEI
                     VNLKVDGREIAELLSQSGIFPAYHMSKKSWVSVLLDETVEDQVVFALLEKSRYLVGPK
                     SYKAEQGSDYWVIPANPKVYDIDTEFAENKVVYWPQKSTIQAGDIVAIYVTAPVQAIR
                     YVCRVLGANLENHGESDIPTGKKLMQVELLAQFSDDVLQRARMMDLGVRAVRGPRRLT
                     EGVIEVLTSEVKNLH"
     gene            4754..5146
                     /locus_tag="U1099_001473"
     CDS             4754..5146
                     /locus_tag="U1099_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEM4867788.1"
                     /translation="MSKKIISVVAAAIEKDGKIFCAQRPEGKSLGGYWEFPGGKLEAN
                     ETPEEALVREIKEEFDSEIEIVSFINEASYEYDFGIVTMKTYLSKLVTGDLTLLEHQD
                     SKWLPIEDLETLEWAPVDIPAVKCLQQK"
     gene            complement(5170..8043)
                     /locus_tag="U1099_001474"
     CDS             complement(5170..8043)
                     /locus_tag="U1099_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM4867789.1"
                     /translation="MSNNNLFQDQLLHSLQYGFIDREIFQEGVYSPKILINDTETHRY
                     VLNDIQEELSKAQSFAFSVAFITQSGLALIKSQLSDLADEGIQGRILISPYLDFNDPI
                     AMKELLKLKNVQVRITPVNLQMHAKFYLFEQKDKQVIISGSSNLTHTALKINYEWNVK
                     LTSTHNGDFIQNAKEEFDRIWEKSIVLDLEAINAYSARRQTVIKTAMIRDESIASYQE
                     TIRPNKMQEQALEGLATVRKNGAQKALVISATGTGKTYLSAFDVKQYNPDRMLFIVHR
                     EQILQKSLKDFQKVLGFEDDEGCIYKSGMDISQKRYIFATIQTISRADNLQAFDPTFF
                     NYILIDEVHKAGADSYKKVMNYFQPDFYLGMTATPERTDGQNIYELFDYNIAYEIRLQ
                     EALDNDMLCPFIYYGVSDIKIDGQLIDEKATFSNLVSEQRVNHILEKIAYYGVSGEAV
                     KGLIFCSSVNEAKELEGQFNARGLRTRALSGEHSQKERQRVVQDLENGNLDYILTVDI
                     FNEGIDIPSVNQVVMLRNTQSSIIFIQQLGRGLRKHDSKEYVTIIDFIGNYQNNYLIP
                     VALFGDQSMNKDNYRREVREPNILKGLTTINFEKVAKEQIFSSITNTTLSSIKILRDA
                     FKDLENKLGRVPYLSDFIKLNSIDPLVFFENSSFQNYGDVINKFSEETILLSEVEKRF
                     LNFITFEILNGKRKHELYLLKLLVENEGLVSSDKWFECIIEHDLDFQTDVLDSVTRVL
                     DLSFLKAQEQKKYGTDALVTFDGELYRLNEEIQESLVNNTKFTQLFLDVIETGILKSK
                     DYPEIFTLGQKYTRREVLKLMNVAKDEPPLNIGGYKIDKDTNSCPIFITYHKSEDISD
                     TIKYDDELLNESTLKWFSKNKRTLDSNDVSTITQSNENGLRLELFVIKDDSEGGEFYY
                     LGPLSYVENSASEVMKNGDSVVQMLFKLDSPVETNLYKYLTDK"
     gene            complement(8128..>8873)
                     /locus_tag="U1099_001475"
     CDS             complement(8128..>8873)
                     /locus_tag="U1099_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867790.1"
                     /translation="NKSLKEIVSIFTSSRRKTNKDEIRLPYRNGILHGRDINFGNEYV
                     SCKCVSLLFAVADWIKQKTNEVERKDSFVKSQTNESIVDLINRYQELKKFEDEIANWK
                     RRDIILGTDIPVSGVRDDYGEYSYLYPILDMFEYWRDGNYGQLSKLLEDAKNMNTSNG
                     KWAGQIREIFNEKKYNSFEFITIDEKTYAKSEITVKVEWEQDSSIRTESLRFNCVYTG
                     LKEEDNLVAPWRENGEWRLIPLDVEKIFY"
BASE COUNT         2631 a         1933 c         1560 g         2749 t
ORIGIN      
        1 aaataagaat tgcgaaacaa gtcactttcg tagagtacga caagccgaaa gcggcgatgt
       61 atcagagtta atttaaatga ctgtactaag aacttgctat tttggggatt tcattgagta
      121 ttaaaattta tctacataaa aacaccttga aaatgcgctt gtcatttatt ataaataacg
      181 agagcttctt caagatgtat cacagaatat aatttcttta gctaatagat tgccactact
      241 gtccaaaaag ctggataaaa ttccttagta atatcgtatt acggtattca aaaatcaaag
      301 actctgctaa aattttgtat cgtcatttaa ttgataaata tcccgcatcg cccaaaaacc
      361 aatcagaatc attagtagac caacccaaat ttcctttgga aattgcatga ttcttaaaaa
      421 tccaagtacc aaggcaatcg ctaatgctac cagtaaaaat actgccgcaa tcaaattcat
      481 agtcccctta atattgcgag gtgcaataaa aatataaaaa agtactaaca tgatggcgag
      541 gattaaataa aacatgcttc ttctcctaga aattattcct cagtcgctaa ttttttcttc
      601 ttcgcagcct tatcacgctc tgccttgttc aagatttgct tacgaagacg gatagattct
      661 ggcgttactt ccatgtactc atcatcgttc aagaattcta gtgattcttc aagagtcaag
      721 atacgtggtg tcttaattac agacgtttgg tctttggttg ctgaacgaac gttggtcatt
      781 tgtttagcag tcgtgatgtt aacggtcaag tcattttcac gcgagttttc accgataatc
      841 attccttcgt acacttcagt acctgggttt acaaagatgg taccacgctc ttcaatacgc
      901 atgatagagt atgtcgttgc cttaccttga tcgatagaaa cgagagcacc acggtgacga
      961 ccaccgattt caccttgaac aactggcaag tattgatcaa aggtatggtt cataataccg
     1021 taaccacgag tcattgaaag gaattctgtt gaataaccaa tcaaaccacg tgcaggtacg
     1081 aggaagacaa gtcgtgtttg accattacct gtcgcaacca tatccaacat atcaccctta
     1141 cgctcagata gggattggat aacagaacct tggtattctt ctggtgtatc aatttgcacg
     1201 cgctcaaatg gctccatttt cacgccgtcg atttccttga tgataacttc tggacgtgat
     1261 acttgaagct cataaccctc acgacgcatg gtttcaatca agatagacaa gtgcaattca
     1321 ccacgacctg aaacagtcca tttatctgga gagtcagtag ggtcaacacg gagagaaacg
     1381 tctgtttgca actcggccaa aaggcgctct tcaatcttgc gagatgtcac ccatttacct
     1441 tcacgacctg caaacggtga gttgttcacc aagaaagtca tttgaagagt tggctcatcg
     1501 atacgaagaa ctggaagtgc ctcaactgca tctgtcggag tcacagtttc accaacgaag
     1561 atgtcttcca taccagaaac agcaatcaaa tcaccagctt ttgcttcttg gatttcttta
     1621 cgctcaagac cgaagaaacc aaagagtttt gtaacgcgga agtttttcgt tgtaccgtct
     1681 agtttagaaa gggtaacctg gtcaccaact ttaacagtac cacggaagac acgaccgata
     1741 ccgatacgac ctacgaagtc attgtagtcc aaaagcgata cttggaactg caaaggctca
     1801 tctgagttat cgactggcgc tggaatatgc tcgataatgg tatcaaaaat tggtgccatt
     1861 gtttcttctt ggtctgctgg attgtcagac aaagatgacg ttccgttgat agctgatgca
     1921 taaaccactg ggaattccaa ctggtcatca tctgcaccca attcgatgaa gagttcaagc
     1981 acttcatcca caacttcttc tggacgagca gaaggcttgt caatcttgtt aacaacgacg
     2041 attggtgtca agttttgttc aagggctttt ttcaatacga aacgagtctg tggcatggta
     2101 ccttcgtaag catctactac aaggacaaca ccgtcaacca ttttcatgat acgctcaacc
     2161 tcaccaccga agtccgcgtg tcctggtgtg tccatgatgt tgatacgagt accgttgtaa
     2221 gctacggcag tgtttttagc caggattgtg ataccacgct ctttttcgat atcgtttgag
     2281 tccatggcac gctcatccaa ctgcgtacgt tcatcaagtg tttgggattg tttcaataat
     2341 tcatcaacga gggttgtttt tccgtggtca acgtgggcaa taatggcaac gttacggatg
     2401 tcttctctaa gttttgtcat aatatcctct atatatttta atttaactaa aaaagtatat
     2461 catagattgg cgcaaaaatc acagataatc ttgttgtaaa tgttttcaga tagcagattg
     2521 aaagcagaag aaaagagatt gaagccaatc tcttataaaa tattctcaaa attatcgcgc
     2581 acttcttgtg gccaaacgct ggactgcact tcaccaatat gtttcttacg aagcaagaac
     2641 atggccagac gggattgacc gataccgcca ccgatagaca gcgggaagag accattcaaa
     2701 agggcacgat gccaatcaaa ttccaaacgg tcctcgtcac ctgtaatagc aacctggcgc
     2761 ttaagcgctt cctcgtctac acgaatcccc atcgaggaca actcaaaggc tgaaccaagt
     2821 gcttcgttcc aaaccaagat gtctccattg agtcctttgt agcctgcttc tgatgggctt
     2881 gtccagtcat catagtctgg tgcacgtcca tcatgtggtt tcccatctgc cagttctcca
     2941 ccaataccga ttaggaaaac cgcaccgtat tctttggcaa ccacattttc acgctctttt
     3001 ggtgtcaagt ctgggaagtt cttcaccaag tcctctgtgt gaataaaggt gattttcttt
     3061 ggtaaaacag actcaatgtc ataacgagct tcaacggcca attcggtcag acggatagcc
     3121 ttgtaaatct gctctactgt ttctttcaag taagctaaat tccgacgacc atcagggatg
     3181 accttttccc agtcccactg atccacatac accgagtgaa tcggatccaa ggaatcctca
     3241 tctggacgca aggctttcat atgtacaaaa agaccttctc cttcgtgaaa tccaaagcga
     3301 gccaaggtat gacgcttcca ttttgcaagc gaatgaacca cttcataagt agcatctggt
     3361 atcagcttca cattaacggt aactgcattc tccacaccgg acaaattatc ctgcatacca
     3421 tccccgacac gactcaaaat cggcccttgc acttctacga tttctagctt gtctttcaaa
     3481 tactgagtga atgtattttt gacgaaagaa atctcctctt gctgatgaat gaagcttttc
     3541 ttcatacctt acctcctcgt aaatttatac accttattat actctttttt acccacttgt
     3601 aaagtctttt tattaaatat tctgataatt taatggaggt ttttcacttc ggatgtcagt
     3661 acttctatca cgccctctgt caagcgtcta ggaccacgta ccgccctgac tcccaagtcc
     3721 atcatgcgcg ctctctgcaa aacatcatcg gaaaactgag caagcaattc cacctgcatg
     3781 agtttctttc ctgttggaat atctgactct ccatgatttt ccaagtttgc ccccaagact
     3841 cgacaaacat agcgaatggc ctgtacaggt gccgtgacat aaatggcaac gatgtccccg
     3901 gcttgaatgg ttgatttttg tggccaatag actaccttat tttccgcaaa ttcggtatcg
     3961 atatcataga ccttgggatt ggctggaata acccagtaat cagaaccttg ctccgccttg
     4021 taggattttg gtcctacaag atagcggctc ttctcaagga gggcaaacac cacctggtcc
     4081 tccacagtct catccagcaa gaccgacacc caagacttct tagacatgtg ataggctggg
     4141 aaaatgcccg actgacttag cagctctgct atttcccgac catcgacctt gagattgaca
     4201 atctccacct cttctttaga cccgctcccg tccagcttgt ccctcggaat ctgactgacc
     4261 agggcgtacc acttattatt ggaggggtgg cgaaaagccc cataggtcgg caacttggca
     4321 aagggatagt caggaaggtc cccccacttc tccttgatga gctgggctat gcggttgctt
     4381 tggtctttgg aaaatggcag agcgatacag cagacttcct ctacccgagc caaaatggac
     4441 tgataagcct cacgcacctg ccccacaaaa gcccctgctg gcgcagtcac cagatgggcg
     4501 gtatagacct cctccatctc acaatcccag acataggacg acagctgacc agcctcatca
     4561 acctccacca ccgcctcaaa ctgcccctcc atgaagacct cacggtaggt gtaacgattg
     4621 tcggcgagaa taaaaccgaa agggatgagg gaggggaggt gggcaatttg tctagacatt
     4681 ttaactcctt taaagtaata tatagagaaa gtataacaaa atctgtaaaa gaatttattg
     4741 tggaggtcaa atcttgtcaa aaaaaattat tagtgtggta gctgcagcaa ttgaaaaaga
     4801 tggaaaaata ttttgcgctc agcgccctga aggaaagagt ttaggagggt attgggaatt
     4861 tccaggagga aaactcgaag ccaatgaaac tcctgaagaa gccttagttc gtgaaatcaa
     4921 agaagaattt gattccgaaa tcgaaatagt atctttcatc aacgaagcat cttatgaata
     4981 tgattttggt atcgtgacaa tgaaaaccta cctttcaaaa ttagtcacgg gtgatttaac
     5041 tttattagaa caccaggatt ctaaatggct ccccatcgaa gatttagaga cattagaatg
     5101 ggctccagta gatattcctg ccgtcaaatg tttgcaacaa aaatagagat tcgctggaat
     5161 ctctattttt tatttatctg tcaaatactt atacaagttt gtctccactg gtgaatccaa
     5221 cttaaataac atttggacaa ctgaatctcc atttttcatt acttcacttg cactattttc
     5281 aacgtatgat agtggaccaa gataataaaa ttctccacct tctgaatcat cttttatgac
     5341 aaaaagttct aacctcaatc cattttcatt agattgagta atcgtcgata catcattaga
     5401 atctaatgta cgtttgttct ttgaaaacca cttcaaagtt gactcattta gaagttcgtc
     5461 atcgtatttt atggtatcac taatatcctc acttttatgg taagtgatga aaatcggaca
     5521 agaattggtg tccttatcaa ttttgtatcc accaatattt aaaggtggtt cgtcttttgc
     5581 gacattcatt aatttaagca cttcacgtcg agtatatttc tgaccaagtg tgaaaatttc
     5641 aggataatct ttcgatttta gaatccctgt ttcaatgaca tccaaaaata attgagtgaa
     5701 tttggtatta ttcaccaaag attcttggat ttcttcattc aaccgataca gttcaccgtc
     5761 aaaagtaacc agtgcatccg taccatattt cttttgctcc tgggctttta gaaatgatag
     5821 gtccaacact cttgttaccg aatctaaaac atctgtttga aaatctaaat catgctcaat
     5881 aatacattca aaccatttat ctgacgaaac tagtccctca ttttcgacaa gcaacttcaa
     5941 caaatacagt tcatgttttc gtttgccatt caagatttca aatgtaataa aattcaaaaa
     6001 tcgcttttct acttctgata gaaggattgt ttcttctgaa aatttattga tgacatctcc
     6061 gtaattttga aaactagagt tttcaaaaaa gacaagcgga tcaatgctgt ttaattttat
     6121 gaaatctgat agatacggaa ctctacccaa cttattctct aagtctttaa aagcatctcg
     6181 taaaattttg attgatgaga gtgtcgtatt tgtgatagaa gaaaaaattt gttctttggc
     6241 aaccttctca aagttgattg tcgtcaatcc ttttaaaatg ttgggctctc tgacctcacg
     6301 acgataattg tctttgttca tagactgatc gccaaataat gcaacaggaa ttagatagtt
     6361 gttttgatag tttccaatga aatcaataat cgtaacatat tctttcgaat cgtgctttcg
     6421 caagcctcga cctaattgtt gaataaaaat gatactcgat tgggtattgc gaagcatgac
     6481 aacctggttg acactcggaa tatcaattcc ctcattaaaa atatcaaccg tcagaatata
     6541 gtctaagttt ccattttcta agtcttgaac tactctctgt cgttcctttt ggctatgttc
     6601 cccagaaaga gcgcgtgtac ggagtcctct tgcattaaac tgcccctcta gctcttttgc
     6661 ttcatttact gaagaacaga aaatcaatcc ttttaccgcc tcaccactta ctccatagta
     6721 agcaattttt tctaaaatat gattgacacg ttgttcagaa actaaatttg aaaaagtggc
     6781 tttttcatca attaattggc catcaatctt aatgtctgaa acaccgtagt atataaatgg
     6841 acaaagcata tcattatcaa gtgcctcttg taaacggatc tcataggcaa tattatagtc
     6901 aaaaagctca taaatatttt gtccatctgt tcgctctggt gttgctgtca tccctaaata
     6961 aaaatctggc tgaaaataat tcatcacttt tttataggag tctgcacctg ctttatgaac
     7021 ctcatcaatc aaaatatagt taaagaaagt tgggtcaaaa gcttgaaggt tatctgctct
     7081 cgaaatcgtc tgaatagtcg caaagatata cctcttctgt gatatatcca tcccactctt
     7141 ataaatacag ccctcatcat cctcaaatcc taacaccttt tgaaagtctt ttaatgattt
     7201 ttgtaggatt tgttctcggt ggactataaa taacattcga tctggattgt attgtttgac
     7261 atcaaaggca gaaagatagg ttttacctgt tccagttgca ctaatgacca gagctttttg
     7321 tgcaccattt ttcctaacgg ttgcgagacc ttctagagct tgctcttgca tcttgttggg
     7381 acgaatggtt tcttggtaag atgctattga ctcgtcccga atcatcgctg tttttatcac
     7441 cgtctgccta cgagctgaat atgcattgat tgcttctagg tctaaaacaa ttgatttttc
     7501 ccaaatacga tcaaactctt cttttgcatt ctggataaaa tcaccgttat gtgttgaagt
     7561 caatttcaca ttccactcat agttaatttt caaggctgta tgagttaaat ttgaactacc
     7621 agaaatgata acctgtttat ctttttgctc aaacagataa aactttgcat gcatttgcaa
     7681 attgactggt gttatccgta cttggacatt tttcagttta agcaattctt tcatcgctat
     7741 gggatcgttg aaatccagat acggagaaat caaaattctc ccttggatcc cctcatccgc
     7801 caaatctgat aattgacttt taattagggc tagtccagac tgagtaataa aagccacact
     7861 gaaggcaaaa gattgggcct tgcttaactc ttcttgaata tcatttaaaa catagcgatg
     7921 agtctctgta tcatttatta aaattttagg cgaatagacc ccttcttgaa aaatctctct
     7981 atcaatgaaa ccatattgca aagaatgaag aagctggtct tggaataagt tgttgttgga
     8041 catgataggt ttcctttatc tctatataga attattatct gtaaaattca gaaaattgta
     8101 aagtcaggaa acaaattatt taataactta gtagaaaatt ttctccacat ccaagggaat
     8161 gagcctccac tctccatttt ccctccacgg tgcaaccaaa ttatcctcct ctttaagacc
     8221 agtataaacg cagttgaatc tcaaagattc ggttcttatc gaactatctt gttcccattc
     8281 tactttcaca gtaatctctg actttgcata tgttttttca tcaatcgtta tgaattcaaa
     8341 tgaattatat ttcttttcat taaatatttc tcttatttga cctgcccatt ttccattgga
     8401 agtattcata ttttttgcat cttctaataa cttcgaaagt tgtccataat ttccatctct
     8461 ccagtattcg aacatatcta atatagggta taaatatgaa tactcgccat aatcatctct
     8521 tacaccactt acgggaatat cagtaccgag aattatatcc cgtcttttcc aatttgctat
     8581 ttcatcctca aacttcttta gttcctgata tctattaatc aaatccacaa tagattcgtt
     8641 tgtttgtgat ttaacaaaac tatcttttct ctcaacctca tttgtttttt gctttatcca
     8701 atcagcgaca gcaaaaagta atgaaacaca cttgcaagat acatactcat taccaaaatt
     8761 tatatccctt ccatgtaata taccatttct gtatggtaat ctaatttcat ctttattcgt
     8821 ttttcgtcga ctagatgtaa aaattgatac tatttctttt aacgacttat tac
//
LOCUS       DASFSW010000086         8008 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.086, whole genome shotgun sequence.
ACCESSION   DASFSW010000086 DASFSW010000000
VERSION     DASFSW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.086"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            76..588
                     /locus_tag="U1099_001476"
     CDS             76..588
                     /locus_tag="U1099_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEM4867791.1"
                     /translation="MLKRYKFDTQKFKLGMRTLKSGLAVFLVILLFHLLKWDGIQIAA
                     LSAVFSLREDFGKSVHFGSSRVLSNSIGGFYALLYFILSDLFSHNFWVTLIFIPIFTM
                     LTIMTNVSFNNAPGVISGVATMLIIALSVPAGDSIFYALQRVFETFVGVFVSVIVNYD
                     IDKIKKRFLS"
     gene            668..1039
                     /locus_tag="U1099_001477"
     CDS             668..1039
                     /locus_tag="U1099_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM4867792.1"
                     /translation="MREKEFRRSLAIFPIGSVMKLTDLTARQIRYYEDHGLIKPVRNE
                     GNRRLYSLNDMDLLLEIKDFLDEGLNIAAIKKEYANRKAKEKVQAEQKTLTDEDVRRI
                     LHDEFSRQGRFSSPSSIFRSS"
     gene            1066..2412
                     /gene="glnA"
                     /locus_tag="U1099_001478"
     CDS             1066..2412
                     /gene="glnA"
                     /locus_tag="U1099_001478"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEM4867793.1"
                     /translation="MVVTTADIKRDIKEKNVTFLRLMFTDIMGVMKNVEIPATEEQVE
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLNTWTIFPWGDENGRVAGLICDIYTTEG
                     EPFAGDPRGNLKKSLRRMEKEGFSSFNLGPEPEFFLFKMDEQGNPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTEMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDKDGNNAFFDPEDPRGMQLSETAY
                     HFLGGLIKHAYNYTAITNPTVNSYKRLVPGFEAPVYIAWAGKNRSPLVRVPASRGMGT
                     RLELRSVDPTANPYLAMAVLLEVGLDGIEHKIEAPAPVESNIYAMSDEERREAGITDL
                     PSTLHNALKALRKDDVVRSALGDHIYTNFLEAKKIEWASYATYVSQWEIDNYLDMY"
     gene            complement(2635..4314)
                     /locus_tag="U1099_001479"
     CDS             complement(2635..4314)
                     /locus_tag="U1099_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM4867794.1"
                     /translation="MSSVNLKPHEVGVFAIGGLGEIGKNTYGIEYKDEILIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENLDRVKGLVITHGHEDHIGGIPFLLKQANVPIYAGPLA
                     LALIRGKLEEHGLLRDATLHEINHNTELTFKHLKVSFFRTTHSIPEPLGIVVDTPQGK
                     IVCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIM
                     KLIEGIHGRIIFASFASNIFRLQQAADAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKDTFIEPNEIKEYPASEIMILCTGSQGEPMAALSRIAHGTHRQVQLQPGDTVIFSSS
                     PIPGNTTGVNKLINILIEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKIHASLAVDTGVPKDNIFIMENGDVLALTKDSARLAGQFNAQDIYVDGNRIG
                     EIGAAVLKDRRDLSEDGVVLAVATVDFNSKMILAGPDILSRGFIYMRESGELIRESQR
                     VLFNAIRIAMRNKDANIQTVNGAIVNALRPFLYEKTEREPIIIPMILTPDR"
     gene            complement(4318..4548)
                     /locus_tag="U1099_001480"
     CDS             complement(4318..4548)
                     /locus_tag="U1099_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEM4867795.1"
                     /translation="MIYKVFYQESKISPRREQTRALYLDIDAASELEGRIIARELVET
                     NRPEYNIEFIDLLSDNHLEYEKEHADFKITEF"
     gene            4995..5678
                     /gene="tsaB"
                     /locus_tag="U1099_001481"
     CDS             4995..5678
                     /gene="tsaB"
                     /locus_tag="U1099_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEM4867796.1"
                     /translation="MKILALDSSNQALSVALVEDGRLQAETLLAVKKNHSISLMPVVD
                     FLVAQVGWTPKDLDRIVVAEGPGSYTGLRVAVATAKTLAYTLKIDLVGLSSLQSLVPP
                     SLTGLVVPLIDARRNCVYAGLYENGRAVEADKYWSFEDLLSSLSGKEDITFVGEVANF
                     IEQIEQALPTAQYQASLPSAYQLAVIGQDLPAVDVTSFEPNYLKRVEAEENWLKDNQA
                     GSESYIKRV"
     gene            5675..6115
                     /gene="rimI"
                     /locus_tag="U1099_001482"
     CDS             5675..6115
                     /gene="rimI"
                     /locus_tag="U1099_001482"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEM4867797.1"
                     /translation="MIEIKYYDGQENLSEAVLAVMESVYEQSPWTLEQIVTSLASQDE
                     DYYLAYEGQELVGFLAVQTVLDEMEILQIAVKADFQRLGIASQLMAAVMDWEGDIFLE
                     VRESNSAAQALYTRQHFTKIGKRKDYYRHPVEDAVLMKRERDER"
     gene            6105..7112
                     /gene="tsaD"
                     /locus_tag="U1099_001483"
     CDS             6105..7112
                     /gene="tsaD"
                     /locus_tag="U1099_001483"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEM4867798.1"
                     /translation="MKDRLILAIETSCDETSVAVLRNDAELLSNVIASQIASHQRFGG
                     VVPEVASRHHVEVITACIEEALLEAEVTAEDLTAVAVTYGPGLVGALLVGISAAKAFA
                     WANGLPLIPVNHMAGHLMAARAVKELEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLPYPAGRVIDELAHEGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAQQKGETLSNADLSASFQACVMDILMAKTKKALEQYPVKTLVVAGGVAANQ
                     GLRERLAAEITDVEVIIPPLRLCGDNAGMIALAAVSEYNKENLAGWDLNAKPSLAFEN
                     L"
     gene            complement(7149..7985)
                     /locus_tag="U1099_001484"
     CDS             complement(7149..7985)
                     /locus_tag="U1099_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM4867799.1"
                     /translation="MKIQPEFYVFNNRSYVDFYPIQFGMEACKPLHSFGPTMKQHYLF
                     HYIISGSGTFYDTSDQREYHLTAGQGFLISPDAICSYEADEKDPWTYIWLEFDGLKTE
                     HFLRQAGLSKEQPIFSQREMPTSSPVYHEMKQILALHHQKSALVLGHLYLFISCLIDC
                     SLTKTSSKHDENKEFYIREAINFVERNYEKAISVDDLAQVCNLNRHYFSRLFKEQMNI
                     SPQQFIIQYRLSEACELLKNTTKTLQEIAEEIGYSNQFNFSTAFKRHYQISPNRWRKI
                     HK"
BASE COUNT         2110 a         1501 c         2041 g         2356 t
ORIGIN      
        1 ctaaaatgtc cgaatgattc ataacattat gtgcatttag aatcattttt taacaaatca
       61 tgttagaata gaagtatgtt aaagagatat aaatttgata cccagaagtt taaattgggt
      121 atgagaactt taaaatcagg tctagctgta tttttagtca ttttattgtt tcatctttta
      181 aaatgggatg gtattcagat tgcggctctc tcagcagttt tcagtcttcg tgaagatttt
      241 gggaaaagtg tgcattttgg ctcttcccgt gtacttagta attccatagg tggcttttat
      301 gccttgcttt attttattct aagtgatcta ttttctcata atttttgggt gaccttaatt
      361 ttcatcccta tttttacgat gctaactatt atgacgaatg tttcgtttaa taatgctcca
      421 ggtgttatta gcggggttgc tacaatgttg atcattgctc tctcagttcc tgcaggagat
      481 tctattttct acgccctcca aagagttttt gaaacattcg ttggagtttt tgtatctgtt
      541 attgtaaatt acgatatcga taaaataaaa aagcgctttc tttcctaaca gtgtcataaa
      601 atgtgacaca gagggcttga ctgttcgttt tttctgccct ataatagtgt gcagaaagga
      661 gttcattatg agagaaaaag agtttcgacg ttccttagct atttttccaa ttggtagtgt
      721 aatgaaattg actgatttaa cagcgcgtca gattcgttac tatgaagatc acggtttgat
      781 aaaaccagtt cggaacgaag gtaatcgccg cttgtattct ctaaatgata tggacttact
      841 acttgaaatt aaagattttt tggatgaggg cctaaacatt gcagcgatta aaaaagaata
      901 cgccaatcgt aaagcaaagg aaaaggttca agcagaacag aaaactttaa ctgatgagga
      961 tgtgcgtcgt atcctacatg atgaatttag cagacaaggg cgattttcaa gtccaagctc
     1021 gatctttcgg tcgtcgtaat aggtttaatt aagaggagaa aaaatatggt tgtcacaaca
     1081 gcggatatta aacgtgatat taaagagaaa aatgttacat ttctacgtct catgttcact
     1141 gatattatgg gagtgatgaa aaacgtagag attcctgcga cagaagagca ggttgaaaaa
     1201 gttttatcaa ataaagttat gtttgatggt tcgtctattg aaggttttgt acgtattaat
     1261 gaatctgata tgtaccttta tccagattta aacacatgga ctatttttcc ttggggagat
     1321 gaaaatggcc gtgtggcagg cttgatttgt gatatttata cgactgaagg tgaaccgttt
     1381 gcgggagatc ctcgtggaaa cttgaaaaaa tctcttcgtc ggatggaaaa agaaggattc
     1441 tcatccttta accttggtcc agaaccagaa ttcttcttgt tcaaaatgga tgagcagggg
     1501 aatccaactc ttgaagtgaa tgacaagggt ggttactttg acttggcgcc tacagatctt
     1561 gcggacaata ctcgtcgtga aattgtaaat gttttgactg aaatgggatt tgaagttgaa
     1621 gcgagccacc acgaagtagc ggttggtcag catgaaatcg actttaaata tgcggatgtt
     1681 ttgaaggcgt gtgataatat ccaaatcttt aagttggttg ttaagacgat tgcacgaaaa
     1741 catggcttgt acgctacatt tatggctaaa cctaaatttg gaattgctgg ttcaggtatg
     1801 cactgtaaca tgtcactctt tgataaagac ggtaataatg ccttctttga tccagaagat
     1861 cctcgtggca tgcaattgtc agagacagct tatcacttcc tcggtggttt gattaaacat
     1921 gcttataact acactgcaat taccaaccct acagtaaact catacaaacg tttggtacct
     1981 ggttttgaag ctcctgttta tattgcttgg gcaggcaaaa accgttctcc attggttcgt
     2041 gtacctgcat cacgtggaat gggaactcgc ttagaattgc gttctgtaga tccaacggct
     2101 aacccatatt tggctatggc agttcttttg gaagttggat tagatggtat cgaacacaag
     2161 attgaggctc ctgctccagt agaatcaaat atttatgcaa tgtcagatga agaacgtcgc
     2221 gaagctggta ttacagatct tccatcaact cttcacaatg cgcttaaagc cttgagaaaa
     2281 gatgatgttg ttcgttcggc attgggtgat catatctata ctaacttctt ggaagctaag
     2341 aaaatcgaat gggcaagcta tgcaacctac gtttctcaat gggaaattga caactatttg
     2401 gatatgtatt aaacaaagga ccccgacggg tcctttgttc acgagccgag aaattggaaa
     2461 gcgtgtaatg gtccagtgga gtgaaatagc ctgggaagag actgtttcag tctgagctaa
     2521 taaatagcag ggcgagggga tggacatgag cttgaaatct gtctttttac aagctgggtt
     2581 atcttgttaa tataaaataa atcccagagg tctcacctct gggattttag tgtgttatcg
     2641 gtcaggcgtc agaatcattg ggataatgat tggttcacgc tctgtttttt cgtataggaa
     2701 tggtcgtagg gcgttaacaa tggcgccgtt gactgtttgg atattggcat ctttattgcg
     2761 catggcgatg cggatagcgt tgaaaaggac gcgttggctt tcgcgaatga gctcgccaga
     2821 ttctctcata tagatgaagc cacggctgag gatgtctggt cctgctaata tcatcttaga
     2881 attaaaatcg acagtggcta ctgcaaggac aacaccatct tctgaaagat cgcgacggtc
     2941 ttttagaaca gctgcgccga tttcaccgat gcggtttcca tcaacataga tgtcttgggc
     3001 gttgaattga cctgcgagac gtgcagaatc ttttgtcaga gcaaggacat ctccgttttc
     3061 catgatgaag atattatctt ttggaacgcc tgtatcaaca gctaggctgg catgaatttt
     3121 ttgcatgcgg tattcaccat ggacgggcat gaagtatttt ggcttgatta ggcggagcat
     3181 gagtttttgc tcttgttgac caccgtgtcc agaggtatgg atgttgttga tcttgccgtg
     3241 aataacatcg acaccggctt caattaagat attgatgagc ttgttgacac ctgtggtatt
     3301 tcccggaata ggactggacg agaagataac cgtgtcgcct ggctggagtt gcacctgacg
     3361 gtgggtaccg tgggcgatgc gagagagtgc tgccataggc tccccttggc tccctgtaca
     3421 gagaatcatg atctcgctgg caggatactc tttgatctcg tttggctcga taaaggtgtc
     3481 ttttggaacc ttgatgtagc cgagctcgat accgttaaca atggcttttt ccatggaacg
     3541 tccaaagaca gcgatcttgc gtccggtctt aaccgctgca tcagccgcct gttggagacg
     3601 gaagatgttg gaggcaaagg aagcaaaaat gatgcgtccg tgaatgcctt caatcagctt
     3661 cataatggac tgaccgacca ctttttccga gttggtaaag gttgggacct cagcatttgt
     3721 cgagtcggag aggaggcaga gaacgccttc ttcaccgagg gcagccatgc ggtgaaggtc
     3781 tgctggctcg ccaactggag tgaagtcgaa cttgaagtca ccggtgcaga cgatttttcc
     3841 ttgcggggtg tcaacgacga taccaagtgg ttctggaatg gagtgggtgg tgcggaagaa
     3901 gctgaccttg aggtgtttga aggtcaactc agtattatga ttgatttcat gcaaagtcgc
     3961 atcacggagc agtccgtgtt cttccaattt gcctcgaata agggcaaggg ctaaaggtcc
     4021 agcatagatt gggacattgg cctgcttgag taagaaggga atgcctccga tgtggtcttc
     4081 gtgcccgtgg gtaataacca gacctttaac acggtctagg ttttcaacga tgtaggagta
     4141 gtctgggata acgtagtcga tacccaatag gtcatcttct gggaacttga taccggcatc
     4201 gacgatgaga atttcatcct tatactcgat accgtaggta tttttcccga tttctcccaa
     4261 accaccgatg gcgaaaacgc caacttcatg aggttttaga ttgactgatg acatggatta
     4321 aaactccgta attttgaaat ctgcgtgctc tttctcgtat tcaagatgat tgtctgataa
     4381 gaggtcgata aattcgatgt tgtattcagg gcggttggtt tccaccaatt cacgcgcgat
     4441 aatacgacct tccaattctg atgcagcatc gatgtctaga taaagagcac gtgtttgctc
     4501 gcgacgtggg ctaatttttg attcttgata gaatacttta taaatcatgt gtagtatagt
     4561 tcctttcttt ttgactggca actatgaaaa aagcctcaaa cagttgggtc tgtctggtaa
     4621 tatctattca attttctcga gttgtcaatt tattcagtct aaatgtactc ttcattatat
     4681 cataaaatat gctgatggta aaagggtttg gtaggaaaat ggaaaagtgt catgattttc
     4741 agaaattcgg tatttcagaa ggggataaat caaagaaatg agttaggatt ccgagtgtgt
     4801 tgggattact aagactaagt tcaattgtgc tgagaaatgc ctgtcaactg ccagatagtg
     4861 atgtgactta ctagaatgac aaatcggttc gatccattaa cctactagaa atcacaaccg
     4921 attcttttca ttatttgaaa aaaaggtcaa gatagcttac aatagagggt agcattgtac
     4981 agaaagagaa atttatgaaa atactagctt tagatagctc caatcaggct ttatcagttg
     5041 ccttggtgga ggacggtcgt ttgcaggcgg aaaccctgct ggccgttaag aaaaatcata
     5101 gtatcagcct catgcctgtg gtcgattttt tggtagcaca ggtgggatgg acgcccaaag
     5161 acttggatag gatcgtggtg gctgagggac ctggttccta cacgggtctt cgtgtggcgg
     5221 tggcgaccgc caagaccttg gcctacaccc tcaagattga cttggtgggg ctatccagtc
     5281 ttcagtctct ggttcctccc agcctgacgg gtctggtggt gcctctcatt gatgcccgcc
     5341 gtaactgcgt ctatgcaggt ctttatgaaa atggcagagc agttgaggca gacaagtact
     5401 ggtcttttga agacttactt tctagcctgt ctgggaagga agacatcacc tttgtcggag
     5461 aagtagcaaa ctttatcgag caaatcgagc aagctctgcc gactgctcag tatcaagcca
     5521 gtctaccgtc tgcctatcaa ttggcagtaa tcggtcaaga cttgccagca gtagatgtga
     5581 cgagctttga gcccaactat ctcaagcggg tagaggcaga ggaaaactgg ctcaaggaca
     5641 accaagctgg ctcagaaagt tatatcaagc gtgtatgata gagattaagt actacgacgg
     5701 tcaggaaaat ctatcggaag ctgtgttggc agtcatggag tcggtctatg agcaatcgcc
     5761 ttggaccttg gaacagattg tgactagcct agccagtcag gatgaggatt attatctggc
     5821 ctatgagggt caggaattag ttggattttt ggctgttcag acggtgctgg atgagatgga
     5881 aatcttgcag attgctgtca aggctgattt tcagaggttg ggaatcgcca gtcaactgat
     5941 ggctgctgtg atggactggg agggggacat cttcctcgaa gtgagggagt ccaatagtgc
     6001 agctcaggcc ctctatacac gccagcattt taccaaaata ggaaaacgaa aagactacta
     6061 ccgccatcct gtcgaggacg cggtgctaat gaagagagaa cgtgatgaaa gatagattga
     6121 ttttggcgat cgagacctcc tgtgacgaga cctcggtggc tgttttgcga aatgatgcgg
     6181 agcttttgtc caatgtcatt gctagccaga ttgccagcca ccagcggttt ggcggggtgg
     6241 tgcctgaagt ggccagccgt caccatgtgg aagtgattac ggcttgtatc gaggaggcct
     6301 tgttggaggc agaggtgacg gcagaggacc tgacggctgt ggctgtgacc tatgggcctg
     6361 gcttggtcgg tgccttgcta gttgggattt cggctgccaa ggcatttgct tgggctaacg
     6421 gtctgccact cattcctgtc aatcatatgg cagggcattt gatggcggcg cgtgcggtca
     6481 aggagttgga gtttccgctc ttggccctct tggtcagcgg tggacacaca gaattggtct
     6541 atgtatcaga ggcgggcgac tataagattg tcggtgaaac gcgagatgat gcggtcggcg
     6601 aagcctatga taaggtgggg cgggtcatgg gcctgcccta tccagctggt cgggtgattg
     6661 acgaattggc acatgagggg caagacattt atgacttccc tcgggctatg attaaagagg
     6721 ataatctgga gttttctttc tctggtttga aatccgcctt tatcaacctc taccacaatg
     6781 cccagcagaa aggggagacc ttgtccaatg cggacttgtc agcctctttc caagcctgtg
     6841 ttatggacat tctcatggct aagaccaaga aggccttgga gcaataccct gtcaaaacct
     6901 tggtggtagc gggcggtgtg gcagctaatc agggcttgcg agagcggttg gctgctgaaa
     6961 ttaccgatgt ggaggttatc attcccccgc ttcgcctctg cggtgacaat gcaggtatga
     7021 ttgccttagc agcggtcagt gagtacaata aggaaaacct tgctggctgg gacctcaatg
     7081 ccaagcctag tctggctttt gagaatttat agaataaaaa cctcttacgt caaggatacg
     7141 taagaggttt atttgtggat ttttcgccag cgatttggag aaatttggta atgtctttta
     7201 aaagcagttg aaaaattgaa ttggttggag tagccgattt cttcagcaat ttcctgtagt
     7261 gtcttagtgg tgttttttaa gagttcgcag gcttcgctga gtcggtattg gatgataaat
     7321 tgttgaggtg agatgttcat ttgctccttg aatagccgac tgaagtagtg gcgattgagg
     7381 ttgcatactt gtgccaaatc atcgacggat atagctttct catagtttcg ttctacgaag
     7441 ttgatagctt ctcgtatata aaattccttg ttttcatcgt gtttgctact tgttttggtt
     7501 agcgaacagt caatgaggca ggagataaag aggtaaaggt gtccgagaac tagagcgctt
     7561 ttttgatgat ggagcgcgag aatttgtttc atttcatggt agacagggct agaggtgggc
     7621 atttctcgtt gtgaaaagat aggttgttct ttcgagagcc ctgcctgtct gagaaaatgc
     7681 tcagttttca gaccgtcaaa ttcaagccag atataggtcc aggggtcctt ttcgtctgct
     7741 tcgtagctac agatggcgtc tggggagata aggaagccct gtcctgcggt taggtggtat
     7801 tcacgttggt cggaagtatc gtaaaaagta cctgaaccag agatgatgta gtgaaacagg
     7861 tagtgttgtt tcatagttgg gccgaatgag tgaagtggct tacaggcttc catgccaaat
     7921 tgaatggggt aaaaatctac ataggatctg ttattaaaga cgtaaaattc aggttgtatt
     7981 ttcatcgtat tctccatacc aaaagtgt
//
LOCUS       DASFSW010000087         7933 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.087, whole genome shotgun sequence.
ACCESSION   DASFSW010000087 DASFSW010000000
VERSION     DASFSW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.087"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..765
                     /locus_tag="U1099_001485"
     CDS             <1..765
                     /locus_tag="U1099_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM4867800.1"
                     /translation="ISVFPILWIFLSSLKADPMKNPGISLPTDLSFDGYIKVFTELHV
                     LDYFWNSFKVVSISVIISIIMISMSSYVIARMDFRGKKLITAMLYSTLFIPATAMTFP
                     VYRLVNVLGIYNTPVALIFVYSCSGIAMSFFIIKNYFAIIPKELEEAAEIDGASYAQT
                     FWKVMLPIARPGILTAAILAFINNWNEYYWASMLIINKDELTVPALLGQFTTSFNTNY
                     NGLFSAIVVIILPPILLFAFASKYFIEALGGGAVKG"
     gene            762..2096
                     /locus_tag="U1099_001486"
     CDS             762..2096
                     /locus_tag="U1099_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEM4867801.1"
                     /translation="MTKNSLQEINKRTEWFQKDRFGMFIHWGLYSIPGKGEWIRSHQQ
                     LSIDDYQPYFEAFNPVKYNPREWAKAAKAAGMKYMVLTAKHHDGFCLFDSAYTDYKST
                     NTQIRKDLVAEFVESVREEGLKVGLYFSLIDWYHPDYPKYGDMIHPMRENEAFKDEKI
                     DFDNYLTFLHNQVEEIVTKYGKLDILWFDYSYGEMFGEKWRATDLIKLVRKHQPDVVV
                     DNRLETSGEGFGSIVTDNINLYSGDFVSPEQIVPHEGIRNIHGEPIPWELCLTMNNNW
                     AYNPTDYLYKSSRTLIRKLVECVSKNGNMILNVGPDALGIINQESLEILNDFGKWLDK
                     NGESIYGCGVSSLPKPEWGYYTQKGNTVYAHVFEQPIGPLALIGIDESKVKRMSFLHD
                     GSEVKISKSWTTNAYEGICFAQYGDIPHFTYPLPDSVDSVIKIELVGDDSED"
     gene            2086..3963
                     /locus_tag="U1099_001487"
     CDS             2086..3963
                     /locus_tag="U1099_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEM4867802.1"
                     /translation="MKIENKQIARYFKLEDGKFYTSHILNKKLDEPIGNEKNTEFLVS
                     FCDGSQLSSEDFTAHVVEQTNESLLVSFYHSEIQLTIFYSKREDVLAKEVTILSSSKT
                     INYIDVEQFGFANLEQVYIPKQQEDIKEMAGFSGYYVELGQPIYAKSFFFGMEFPMGE
                     NRLVDQTYFSRYYVGHKIKEPKKIWPVVIGAASGFEKASIQQEFFTYISGIAQPSYFR
                     KQYNSWYDHMTAIDEGIIVKSFEEISHGFEDNGVKLDAYVVDDGWCDYQSVWEFNEKF
                     PNGLTKVKALVNNLGASLGLWIGPRGGYNGTEVIMSDWLEAHPEFGSKNALSNDVNIG
                     DFNYLEGMKQKMLDYQKKYDISYWKIDGWLLKPDKPDPSGEFAMHTMTPVYEFLIDLL
                     KDLRAERGDRDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDIQRMITYR
                     DCQYEEFLAKRDIQLPLWSLYNHEPVYASTAHTWYMDHQMYASVEEFEDYLLFISTRG
                     NAFWEFHYSYTMFDQERWKANARAVKWIENNYSTLKHSQMIGGKPSEFEIYGYRCIDE
                     DVGKEILSLRNPASHSATIQLDNINLSDYQLVRGDYKALTETEYELAPYTILIAEKLG
                     G"
     gene            3965..4858
                     /locus_tag="U1099_001488"
     CDS             3965..4858
                     /locus_tag="U1099_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM4867803.1"
                     /translation="MAVKYALGVDIGGTKVAVGLVDGTGHVAYSLKVPSNKESSETLF
                     QCVCTTIRELLNSQQLNIEDVAGIGVGLPGKVDVENGVAVFQNNIPWENFPVVKRLQK
                     EFGNIPIKIDNDVKVAAYAEYRMLSLEADDMFGYITISTGIAATNIINNTILRGSGFA
                     GEIGFMPVRSFGRTSGLEISCSGPAIERQGKQMYGEDLSTKAVFDNWRKGDITASAII
                     ANARDGMVQAIHNMICLLDPKIIVLGGSVAQNNPDFIEDIKTVLGLSLHHEQKHILNN
                     IIISKIDNGNNGIIGSAFLVQ"
     gene            5001..>7933
                     /locus_tag="U1099_001489"
     CDS             5001..>7933
                     /locus_tag="U1099_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEM4867804.1"
                     /translation="MKNNKRKFTLLTIATLASAFLFQNHVFASEVSLSDLNWTTATHG
                     DATSSKTVQKNKPFTAGNENRENKISLKMEDGSIREFEKGIGTVAANPSTISYDISNL
                     GATKFKSFVGIDRTAVPTDNRYSKISKFEVLVDGAVIYTSIDNYPDGIKYDTSAIWLE
                     LDIPSNAQIIEFKSYSGEHTWGDEVVLGNPTFEIPDSSEIQDDAVDVVDQTSPNELPV
                     RENSVYLSDLDWRSASHGDATASKTIQKNKPFTLGNNGSNQKISLKMSDGSIKEFEKG
                     LGTIAAGPSTIQYDISGAGVSRFTTFVGLDRSAGHADSRYANIEKFEIEVDGTVIYST
                     LNEYPDGFNYDTPAIKVDVEIPEGARSIRLNSYSGQQTWGDELVLAGAYFVASGQFRN
                     PNDFEPAPKRREISNTSPLLMMPLYANGSEYSKGNYSFWGEDTLVGKWENIDPELKPY
                     TVIQVHPDDLPHRAGVAQDFYEKILEQAQNYVNPTTGQNEPIPVILTVYTAGNQSHYT
                     AAHWITMEWIDRMYEKYSCLQGIFSTENYWVWAGNVESNAAEYLKLSARHGGYFIWSE
                     QNNGASIEKALGTQGRPIFKETVEKYWQNFIFMFKNTPAHEGNDAPTVSYMTGLWLAD
                     YAYQWGGLMDTWKWYETGKWKLFESSGIGKTQGNRQWLTQPEAMLGAEAINIYLNGGS
                     VYNFEHPAYTYGVRNEESPLYSTVIENFFKYVINNPAPSKEDVLAATKILINGNFSSK
                     RDGHFYVDVNTATHQSPLYTTGRYGVIPAVPSSISSEVLNSKLPDHIQVVSLNDGSMS
                     SAANRRNLLNGLYPEEYTGNIFGDEIDNRAFIYNYEYNRDNDQTGSFELSGKEFDVTL
                     KSHSFTIVENIATGLNIKQNNFRINKDSLWEGASNSTQARALPQLSKSDAINWVFNTY
                     IHNTPSSEQRETVYVLKNVSKQPTVEILNSSDSNFVTPSVEYNSESKTATIRIVSNGY
                     VDLNI"
BASE COUNT         2601 a         1261 c         1660 g         2411 t
ORIGIN      
        1 atttcagtgt ttccaatttt atggatattt ctatcctcat taaaagcgga tcctatgaaa
       61 aatccaggca ttagtttgcc aactgattta agttttgatg gttatatcaa ggtttttact
      121 gagttacatg tattggacta tttttggaac agttttaaag ttgtttccat atctgtgatt
      181 atcagtatta tcatgatttc catgtcatct tatgttattg ctcggatgga tttccgtggg
      241 aaaaagctga taacagcaat gttgtactcc actctattta tccctgcaac agctatgact
      301 ttccctgtct atcgtttggt aaatgttttg gggatatata atacgccagt agctctgatt
      361 tttgtctact cttgtagcgg tattgccatg agtttcttta tcataaagaa ttactttgca
      421 ataataccta aggagttgga agaagctgct gaaattgatg gagcgagcta tgcccagaca
      481 ttttggaaag taatgttacc tattgctaga cctggtattt taacagcagc aattttagct
      541 tttataaata actggaatga atactattgg gcatcaatgt tgatcatcaa taaagatgag
      601 ctgacggttc cagccttgct tggtcaattc acaactagct ttaatactaa ctataatggt
      661 ctcttttcag caattgttgt gattatctta ccaccaattt tactatttgc atttgcaagt
      721 aaatacttta ttgaagccct tggaggagga gcagtaaaag gatgacaaaa aatagtttac
      781 aagaaattaa caaacgtaca gagtggttcc aaaaagaccg ctttggaatg ttcattcact
      841 gggggctcta ttcaatccct ggaaaagggg agtggatccg cagtcatcaa caattatcca
      901 ttgatgacta tcagccatat tttgaagcat ttaatcctgt aaagtacaat ccgagagagt
      961 gggcgaaggc agcaaaagca gcaggtatga aatatatggt cttgaccgcc aagcaccatg
     1021 atggattttg cttgtttgac tcagcctata ctgactacaa atctacaaat acacaaatca
     1081 ggaaagactt agtagctgaa tttgttgaat cagtacgaga agaaggatta aaagtaggtc
     1141 tatacttcag tctgatagac tggtatcatc cagattatcc gaaatatggc gatatgattc
     1201 atcctatgcg tgagaatgaa gcctttaaag atgagaagat tgactttgac aactacttga
     1261 ctttcttaca caatcaagtg gaagaaatcg taaccaagta tggcaaactg gatattctat
     1321 ggtttgacta ttcttacgga gaaatgttcg gcgagaaatg gcgtgcaaca gatttgatta
     1381 agcttgtacg taagcatcaa ccagatgtag ttgttgacaa tcgtttagaa acatcaggag
     1441 aaggatttgg cagtattgta acggataata tcaatcttta ttctggagac tttgtcagtc
     1501 cagagcagat tgttccgcat gagggaattc gaaatataca tggagagcca attccttggg
     1561 agctttgctt aactatgaat aataactggg cctacaatcc tacggattat ctctacaaat
     1621 cgtccaggac acttattcgt aagttggttg agtgtgtaag taaaaatggg aatatgattc
     1681 tgaatgtagg tccagatgca ctaggaatta tcaaccaaga gagtctggaa attctaaatg
     1741 attttggaaa atggctggat aagaatgggg agtccattta tggttgtggc gttagttcat
     1801 tacctaagcc agaatggggc tactatactc aaaaagggaa tacggtttat gcccatgtct
     1861 tcgaacagcc aattggtcca cttgccttga ttggtatcga tgaaagcaaa gtgaaaagga
     1921 tgagtttcct tcatgatggt agtgaagtga agatttctaa gagctggaca actaatgctt
     1981 acgaagggat ttgttttgcc cagtacggcg atattcctca ctttacttat cctttgccag
     2041 acagcgtaga tagtgtgatt aagattgaat tagtaggtga tgatagtgaa gattgaaaat
     2101 aaacagatag cacgctattt taaattagaa gatgggaagt tttatacttc ccatatcttg
     2161 aataaaaagc ttgatgaacc cattggaaat gaaaaaaata cggaattcct tgtttctttc
     2221 tgtgatggaa gccagctcag ctctgaggat tttacagctc atgtggtcga acaaaccaat
     2281 gaaagtcttt tggttagttt ctatcattca gaaatccagt taaccatttt ttattcaaaa
     2341 cgtgaagatg tacttgctaa agaggttact attctgtcgt cttctaaaac aattaactac
     2401 attgatgtgg agcagtttgg ttttgcaaac ttagagcagg tatatattcc gaaacagcaa
     2461 gaagatatta aggaaatggc tggattttct ggctattatg tagagttggg acaaccaatt
     2521 tatgccaagt ccttcttctt tggtatggag tttccaatgg gagaaaatag actggtagat
     2581 caaacctatt tttctcgcta ctatgtcggt cacaaaatta aagagcctaa aaaaatctgg
     2641 ccagttgtta ttggtgcggc ttctggattt gagaaagcaa gcattcaaca ggaattcttt
     2701 acttacatta gtggaattgc tcagccaagt tacttcagaa aacaatataa ttcttggtat
     2761 gaccatatga ctgccattga tgaagggatt attgtaaaga gttttgaaga gatttcacat
     2821 ggctttgaag acaatggtgt gaagttggat gcctatgtcg ttgatgatgg ctggtgtgat
     2881 taccaaagtg tttgggagtt taatgaaaag ttccctaacg gattaacaaa ggtaaaagct
     2941 ctcgtaaata atttaggtgc aagtcttggt ctttggattg gaccacgtgg aggctataac
     3001 ggtacagaag ttatcatgag tgactggttg gaggcacatc cagaattcgg tagcaagaat
     3061 gccttgtcaa atgatgtcaa tattggtgac ttcaattatt tggaaggcat gaagcaaaaa
     3121 atgttggatt accagaaaaa gtatgatatc agctattgga aaattgatgg ttggttgctg
     3181 aagccagaca aaccagaccc tagtggcgag tttgccatgc acactatgac tcctgtctat
     3241 gaattcttga ttgacctctt aaaagattta agggcagagc gaggagatag agattgctgg
     3301 ttgaacttga cttcttatgt caatccaagc ccatggttct tacaatgggt caacagcctt
     3361 tggatacaaa tttcgcagga tgttggcttt acagaaaatg ctgggaatga tattcaacga
     3421 atgattacct accgtgattg ccaatatgaa gaatttttag ctaaacggga tattcaattg
     3481 ccactttgga gtctttataa ccatgaacct gtctatgcga gcacggctca tacctggtac
     3541 atggaccatc aaatgtatgc aagtgtagaa gagtttgaag attatctcct atttatttcc
     3601 actcgaggaa atgctttttg ggagtttcat tattcctata caatgtttga tcaggaacgt
     3661 tggaaggcca atgctcgtgc tgtcaaatgg atagagaaca attactcaac tttaaaacat
     3721 agtcaaatga ttggtggcaa accgtcagag tttgaaattt atggttacag atgtattgat
     3781 gaagacgtag gaaaagaaat tctttcgctt cggaacccag ctagtcattc agcgactatt
     3841 cagttagata atatcaatct ttccgactat caattggtta gaggagatta taaagctctg
     3901 acagaaacag agtatgaact agctccatat acaattctga ttgcagagaa actaggaggg
     3961 taatatggca gttaaatatg ctttaggtgt agatattggt ggtacaaaag ttgctgtggg
     4021 actggtagat ggtacaggtc atgtggctta tagtttaaaa gtgccaagca ataaagaatc
     4081 atcagaaacc ttatttcaat gcgtttgtac aactattcga gaactactaa acagtcagca
     4141 actgaatatt gaggatgttg cagggattgg agttggcttg ccgggaaaag ttgatgttga
     4201 aaatggagtt gcagtttttc aaaacaacat tccatgggag aattttcctg ttgtgaagcg
     4261 acttcaaaaa gaatttggaa atattccaat aaaaattgac aatgatgtga aagtagcggc
     4321 ctatgcagag tatcgaatgc tttcattaga agcagatgat atgtttggct atatcactat
     4381 ctctacaggt atcgctgcta ccaatatcat aaataacaca attctaagag gctctggctt
     4441 tgcgggcgaa atcggtttta tgccagttcg gagtttcgga agaacaagtg gtctagaaat
     4501 ctcctgctca ggaccagcca ttgaacgcca agggaagcaa atgtatggtg aagatttgtc
     4561 aaccaaggca gtgtttgata attggcgtaa aggagatatt acagcatcgg ctattatcgc
     4621 taatgcgaga gatggcatgg tccaagcgat tcataatatg atttgtttac tggatcctaa
     4681 aataatcgtt ttgggtggta gtgttgcaca aaataaccca gattttattg aagatataaa
     4741 gaccgttttg ggattatctt tacatcatga acaaaaacat attttgaata atattatcat
     4801 ctcaaaaata gataatggaa ataacggaat aattggttct gcattcctag tccaataata
     4861 gagaagaaat atgatgaaaa tagatagttc ataatacttt caattttata gtttagtttg
     4921 ttttccaata tcagaaaaaa tggactagga aatgttggga agcaaaagtt ctcttttatc
     4981 aagaataaga ggtaaaacaa gtgaaaaata acaagagaaa atttacactt ttaacgattg
     5041 caacattggc ttcagcattc ttgtttcaaa atcatgtgtt tgcatctgag gtttcattgt
     5101 ctgatttaaa ttggactacc gcaactcacg gtgatgctac ttcatccaaa actgttcaaa
     5161 agaataaacc atttactgca gggaatgaaa accgtgagaa taaaatttca ttaaaaatgg
     5221 aagatggctc aattagagaa tttgagaaag gaatcggaac tgttgcggca aatccttcta
     5281 caattagcta tgacatttct aacctaggag caacgaaatt taaatccttt gtaggaattg
     5341 ataggacagc tgttcctacg gataatagat attccaaaat tagtaaattt gaagtgcttg
     5401 tagatggggc tgtaatttat acatcaattg acaattatcc agatgggatt aaatacgata
     5461 catcagcgat ttggcttgaa ttagatattc catctaatgc acaaataatt gaatttaaga
     5521 gttactcagg agaacataca tggggtgatg aagttgtgtt aggtaatcca acttttgaaa
     5581 ttcccgatag ttcggaaatt caagatgatg ctgtagatgt tgtagatcag acttctccaa
     5641 atgaactacc tgttagagag aattctgttt atctttcaga cttagattgg cgaagtgcta
     5701 gtcacggtga tgccacagct tcaaaaacta ttcaaaaaaa taaaccattt acgctaggga
     5761 ataatggaag taatcagaaa atttctttaa aaatgtctga tggatctatt aaggagtttg
     5821 aaaaaggact aggtacaatc gctgctggac catcgacaat acaatatgat atttctggag
     5881 caggagtatc acggtttacc acatttgtag gattagatag gagtgctggt catgcagata
     5941 gtaggtatgc taatatagaa aaatttgaaa ttgaagttga tggtactgta atttactcga
     6001 ctttgaacga atatccagat ggttttaatt atgatactcc agcaattaaa gttgatgttg
     6061 aaattccaga aggtgctcgt tctattcgtt taaatagtta ttctggtcaa caaacctggg
     6121 gcgatgagtt agttttagca ggtgcttact ttgtagcaag tggacaattc cgtaatccca
     6181 atgactttga gcctgcacca aaacgtagag aaatttctaa cacaagtcca ttacttatga
     6241 tgccattata tgctaatggt tcagaataca gtaaaggaaa ttattcattc tggggagagg
     6301 atactttagt tggtaaatgg gaaaatattg atccagaatt aaagccttat acagtaattc
     6361 aagttcaccc agatgatttg ccacatagag caggagtagc tcaggatttt tatgagaaaa
     6421 ttctcgaaca agctcaaaac tatgtgaatc caaccaccgg tcaaaatgaa cctattccag
     6481 ttatcttaac tgtatacaca gcaggcaacc aatcacatta tactgctgca cattggatta
     6541 ctatggaatg gattgataga atgtatgaaa agtattcttg tcttcaagga atcttttcaa
     6601 cagaaaatta ttgggtctgg gctggaaatg ttgaaagtaa tgcggctgag tatttaaaat
     6661 tgtcagcaag acacggagga tatttcattt ggtctgagca aaacaatggt gcctccattg
     6721 aaaaagcttt aggaactcag ggaagaccga tctttaaaga gacagtcgaa aaatattggc
     6781 aaaactttat tttcatgttc aagaataccc ctgcacatga aggtaatgac gctccaactg
     6841 taagttacat gacaggccta tggcttgctg actatgcata ccagtggggt ggattgatgg
     6901 acacttggaa atggtatgaa acaggaaaat ggaagttgtt tgaaagctct ggaattggaa
     6961 aaacacaagg gaatagacaa tggttaactc aaccagaagc catgttagga gcagaggcta
     7021 ttaatatcta tttaaatggt ggatcggtat ataattttga gcatcctgcc tatacctatg
     7081 gtgtaagaaa tgaggaatca ccattatatt caactgtaat tgaaaacttc ttcaaatatg
     7141 taataaataa tcctgcacct tctaaagaag atgtactcgc tgctacaaaa attttgataa
     7201 atggaaattt ctcatctaaa cgagatggtc atttctatgt tgatgtcaat acagcaacac
     7261 accaaagtcc actatataca actggtcgat atggtgtaat ccctgctgta ccatctagta
     7321 tttcttcaga agtattgaat tcaaaattac ctgaccatat tcaggttgtc agtctaaatg
     7381 acggcagtat gtcttcggcg gctaacagaa gaaatttatt aaacggccta tatccagaag
     7441 agtatactgg aaacattttt ggtgatgaga ttgacaatag agcatttatc tataattatg
     7501 agtataatcg cgacaatgac cagactggtt cattcgaact tagtggtaaa gagtttgatg
     7561 taactttgaa atctcactcg ttcacgattg tagagaatat tgcaacagga ttaaatatca
     7621 aacaaaataa cttccgaatc aataaagatt cattatggga aggtgcttca aattcaacac
     7681 aagctagagc attacctcaa ctatcaaaat ctgatgctat aaattgggta tttaatactt
     7741 atatccataa tactccaagc tccgagcagc gcgagacagt atatgtattg aagaatgtaa
     7801 gtaaacaacc aactgttgag atactgaatt ctagtgattc taattttgta actccaagtg
     7861 tagaatacaa ttctgaatca aaaactgcga cgattagaat cgtatcaaat ggttatgttg
     7921 atttgaatat taa
//
LOCUS       DASFSW010000088         7882 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.088, whole genome shotgun sequence.
ACCESSION   DASFSW010000088 DASFSW010000000
VERSION     DASFSW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.088"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            120..332
                     /locus_tag="U1099_001490"
     CDS             120..332
                     /locus_tag="U1099_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM4867805.1"
                     /translation="MSQQHQKWIELVEKRLAEKGWTKADLTQAVGLRSQGTITDLLTK
                     GKGSADLKLRVSKLLGIREPWEKFEE"
     gene            353..1072
                     /locus_tag="U1099_001491"
     CDS             353..1072
                     /locus_tag="U1099_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEM4867806.1"
                     /translation="MHEIINVNLNDNHEPVVSGRQLHEALGVKTPYSMWFDRMVEYGF
                     TENQDFLLNNFVKQTGRGGHNKVDHIIKLDMAKEIAMIQRTDRGKQVRQYFIQVEKDF
                     NSPEKIMARALLLADKQVHQLEAQIEADKPKVLFADAVSASHSSILVGDLAKLISQNG
                     FKIGANRLFAWLRENGYLIKRKGSDWNMPTQKSMELGLFEIKETTITHADGHISISKT
                     VKVTGKGQQYFINKFLADDVA"
     gene            1429..1677
                     /locus_tag="U1099_001492"
     CDS             1429..1677
                     /locus_tag="U1099_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867807.1"
                     /translation="MTEAILTLGIFAVPILTAAVVEQRKAEKRRMRKEFEEIRRRDYL
                     YGFKAGMGYQSTCDIEKARNGLKRNAQQVDKEIARYAN"
     gene            1667..1816
                     /locus_tag="U1099_001493"
     CDS             1667..1816
                     /locus_tag="U1099_001493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867808.1"
                     /translation="MRINEGIGRENLIDQIVYITGKARQFYQAMSPYELAMELKIAKL
                     QAGLI"
     gene            1949..2293
                     /locus_tag="U1099_001494"
     CDS             1949..2293
                     /locus_tag="U1099_001494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867809.1"
                     /translation="MTSIERIREYYRDHPNASSKEASEALKIKENTIKASVSKDVKNR
                     RAVRLDNGGIDYTDFFEKDGRLKAFREYQKEILEEQIEVLREANRREIDSNQIRLNAR
                     EIRMLLNDLARL"
     gene            2290..2478
                     /locus_tag="U1099_001495"
     CDS             2290..2478
                     /locus_tag="U1099_001495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867810.1"
                     /translation="MTRQDLIELMESDAAIGIKDFLAMHDRYVACMITHKQGYGHDQL
                     EYIAAYVKFLENHFMEDL"
     gene            2475..2777
                     /locus_tag="U1099_001496"
     CDS             2475..2777
                     /locus_tag="U1099_001496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867811.1"
                     /translation="MKIYIADDKRLIVEPSWFDCFDHTGKEYVNLPKAKIQLKSKITD
                     VIESEIRLAIAEVIKEYQAEMADLPLEDIFNEKRKQVRDSYDTEQAVADVIERWQK"
     gene            2774..3463
                     /locus_tag="U1099_001497"
     CDS             2774..3463
                     /locus_tag="U1099_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4867812.1"
                     /translation="MKITKATDITLNDSCYLIYGNPGFGKTSALKYIPGKTLVIDIDK
                     SSKVLNGCENISIAEVDTHKIWDEWLNVVKELLKGAAEPFDTIVVDNVSELFRACLAN
                     LGREGKNHRVPSQADYQRVDFTILDSLRALLQLKKRIVFTAWETSDQWTDENGMIYNR
                     AMPDIRSKILNNFLGLTDVVARLVKKTTEDGEEVRGFILQPSASVYAKNRLDDRKGCK
                     VDELFTTELPS"
     gene            3432..4844
                     /locus_tag="U1099_001498"
     CDS             3432..4844
                     /locus_tag="U1099_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM4867813.1"
                     /translation="MSFSLRNYQVELILDIKKSMLAGHRKIMVQSPPRSGKTVCMAHI
                     AKNATNKQKTVLFFSHRKEINEQVFETFSRAGVDMGLVYIGTVGSIVRKLGKLPMPTL
                     VLVDEAHHIKASQYQQILKYYHQAVQLFFTGTPIRLDGSGFDDMADDLVVGKSILWLQ
                     EHGNISEFDYYSINLLDQAKLRKRQGEYTNQSIDDSFDFKQQHGDYLSHYERLAKGKQ
                     AIVYCHSVEYAERVFKRFFEAGYQSAVVSGKTPKVERERAMRAFRDGEVTIMVNVNLF
                     TEGIDLPGVDVCIMLRPTASLSLYLQFAMRALNPRDGKRAILIDHVGNHIRHGLPNDD
                     RYWTLEGVDKRKSSSKEKEEAPKTCENCFATFYRDRIVDGKCPYCGEPLKIIKDIEKE
                     ATNETLTLINQGMEFVSIRGEMIEVTREEALVYKRVKRYGKKYERCESLAELKAFRII
                     HGYAPGWLWHKQKELNLWRN"
     gene            4847..5317
                     /locus_tag="U1099_001499"
     CDS             4847..5317
                     /locus_tag="U1099_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEM4867814.1"
                     /translation="MGLFTVNYEAAEQFSSIEDGTYEVFISHVEQSASKGGTDFLDIR
                     LKIREDFQQKFRNNLIFDKIWINKETLQYPEFALQRYAKAVKLPENIEIQTVEQFLNL
                     IKGKNLKVTVKNEQSEYNGKTYDNLNIKKYEQSELPPVAVQASQPVVDDLDLPF"
     gene            5322..6128
                     /locus_tag="U1099_001500"
     CDS             5322..6128
                     /locus_tag="U1099_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEM4867815.1"
                     /translation="MAGMVEYALHYARLGFSVIPIDKRSKRAITAYKDKTFSELEIKR
                     LWRDNPDANIAVKTTDFFVIDIDVRDDVDGYSSFEEWELKQYIPATLQATTPSGGRHI
                     FLKKPKGVQISQDIKVRPGIDIKAHPNNYVLVAPSNNPRGKYVWDQSVEEMAEAPIEL
                     LDILQAGKKPSKINFTTKYNPEYSSKTAKLFEQIVFGLGDEGGRNNNLASLIGGLLIR
                     GVDEEAAYMLAKIANHYTPSPLSQQEVDRTFESMLRKEFDRRSGIGYSED"
     gene            6193..7656
                     /locus_tag="U1099_001501"
     CDS             6193..7656
                     /locus_tag="U1099_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM4867816.1"
                     /translation="MRLACRDYRNDWLEKAKWKETQYGTLEQINDAPKRLTELAVAEG
                     LEQILYVINLPNDRVAVYDPDAGYYHKDPSFAYKVIRLLEPTFTETRSKNVLFMLAAT
                     KRKYLYDGFSCDFSIGDYQDPKRFILVKNGIFDKQLKKMSGFTHRFVAFSTIETEYDP
                     FAESPNIDGWDVDDWLLDLMSGDKDLVHLLWQVISASLNGNYSYRKSIWFVGEGNDGK
                     GTVQQLITNIVGIRNVATLKLNQFSERFALSMIEGKTVIIGDDVQAGVYIDESSNFNS
                     VVTGEPVLVEEKNKQPYSTVFKKTVIQSTNELPRFKNKTNGTYRRFLIIPFRKTFSAK
                     EDNWQIKDEYINRDDVKQYVLKKALELNFTRFSEPQATLDVLEEFKSSNDTVKAFIDE
                     WFGTFQSERLPVRFLWWLYQEWCKEEGITKVAKGKFERQLIKLLPAEWEKKRAKPTRR
                     FKPSLDVPRRYTGFYWDNDNDPNTTAVCLDKKLLVTD"
BASE COUNT         2486 a         1379 c         1920 g         2097 t
ORIGIN      
        1 cggtgttttt cttatatctt gattttagac tatagtttgt aaaaagtcaa gtggaaccta
       61 taaaatttcg caaaaacttt cgctttactt tctaactaat aaaaagaaag gagaagttta
      121 tgagtcaaca acatcaaaaa tggatagaac tggtggaaaa acgattggct gaaaaaggct
      181 ggacaaaagc tgatttgaca caagctgtcg gattacgtag tcaaggcaca atcacagatt
      241 tgttgaccaa agggaaaggg agtgctgatt tgaaattgcg tgtttcaaaa ttattgggca
      301 tccgtgagcc gtgggaaaaa tttgaagaat aataaatatt gaaaggagca ttatgcacga
      361 aattattaac gtaaatctaa acgacaacca tgagcctgtt gtgtctggtc ggcagttaca
      421 tgaggctttg ggggtcaaaa caccatactc tatgtggttt gaccgaatgg ttgagtatgg
      481 ctttacagaa aatcaagatt ttttgcttaa caattttgtg aagcaaacag gtcgcggagg
      541 tcacaacaag gttgaccaca ttatcaagct ggacatggcc aaggaaattg ccatgatcca
      601 acggacagac cgtggcaagc aggttcgtca gtactttatc caggttgaaa aggacttcaa
      661 cagtccagag aagattatgg ctcgcgctct gctattggct gacaagcagg tgcatcagct
      721 ggaagcacag attgaggcgg ataagcccaa ggtgctattt gcagacgctg tgagtgctag
      781 tcactcatct atcttggttg gagacctagc taagcttatt agccaaaacg gctttaaaat
      841 cggcgcaaat cgcttgtttg cgtggttgcg tgagaatggc tatctgatta agcgcaaggg
      901 cagcgattgg aatatgccga cgcagaagtc tatggaacta ggtttatttg aaatcaaaga
      961 gacgactatc acacatgctg acggtcatat ctcgattagc aaaactgtaa aggttacagg
     1021 caaaggtcag cagtatttta tcaataaatt tttagctgat gatgttgctt gaaaaacaaa
     1081 aaaagcctga cggcaatcag gctcttacta aaatgactta cttgaattat aacacacgaa
     1141 agcgaggttt gacaagatgg atgacattgc tgaaagcctc atatcacgtt ttatcagtca
     1201 gttaaaagtt agattggtgg aagtgtttga ggtgtttaac ttagaactag caatgccttt
     1261 gctactcaac agtaagcaat gcaagaagtt gctaggaatc atgaatgaat cggaattcca
     1321 gagggtatca cacctaaagg attttccaag gattgaaaag aaagggtcgc acccacgatt
     1381 tccacgggat gccgtggttg agtggatgcg tgtaaattgg aggttgatat gactgaagca
     1441 attttaacat taggaatctt cgctgtgccg attttgacgg cggcagttgt ggaacaacgg
     1501 aaagcagaga aaagacggat gcgcaaagag tttgaagaaa ttcggcgtag agattatctg
     1561 tacggcttta aggcaggcat ggggtatcag agtacctgtg acattgaaaa agctcgcaac
     1621 gggctgaaac ggaacgctca gcaagtagat aaggagatag cacggtatgc gaattaacga
     1681 gggtatcggc agagaaaacc ttattgatca gattgtgtat atcacaggta aagcgcgaca
     1741 gttttatcaa gccatgtcac cctatgagct ggctatggaa ctgaagatag ctaaattaca
     1801 ggcagggttg atatgacaat ttacgacaga gaatatgtgt gtgacgaatg tcttgagcaa
     1861 tgggaatcac gctcagaaga gccgatggta ttttgtccat attgtcaatc ggatgtagaa
     1921 ccgagaatca tcggtaaatg gagggcttat gactagtatt gaaaggatta gggaatacta
     1981 tcgcgaccac ccgaacgcat cttctaagga agcgtcagag gctttaaaaa ttaaagagaa
     2041 cactatcaaa gcttcggttt ccaaagatgt gaaaaatcgt cgggcagttc gtttagataa
     2101 cggtggtatt gactatacag atttttttga gaaagatgga aggttaaaag cattccgtga
     2161 gtatcagaaa gagattttgg aagagcaaat tgaagtccta cgggaagcaa atcgtagaga
     2221 gattgatagc aaccaaattc gcttgaatgc tcgtgagata cggatgttgc tgaatgattt
     2281 ggctagatta tgacaagaca agatttaatc gaattgatgg aaagtgatgc agcaataggt
     2341 atcaaagatt ttctggctat gcatgatcgc tatgttgcct gcatgatcac tcacaaacaa
     2401 ggttacggcc atgaccagtt ggagtatatc gctgcatacg ttaaattttt ggaaaatcat
     2461 tttatggagg atttatgaaa atctacatag cggatgacaa gcggttgatt gtcgaacctt
     2521 cgtggtttga ctgtttcgac catacaggga aagaatatgt gaatctcccc aaagctaaga
     2581 ttcaattaaa atcaaaaatc actgatgtaa ttgagtcaga gattcgtcta gcaatcgcag
     2641 aagtcatcaa agaataccaa gctgaaatgg cagatttacc gttagaagat attttcaacg
     2701 aaaagcgcaa gcaagtgcgt gattcatacg acacagaaca agcggttgct gatgtgatag
     2761 aaaggtggca gaaatgaaaa ttacaaaagc gacagatatt acgttaaatg attcctgtta
     2821 tctcatctat ggaaacccag ggtttggcaa gacttcggca ttaaaatata tcccgggcaa
     2881 gacattggtg attgatattg acaagtcatc caaggttctg aacgggtgcg agaatatttc
     2941 tattgcggaa gtagacacgc ataaaatctg ggacgaatgg ttgaatgttg tcaaagaact
     3001 actaaaagga gcggctgaac cattcgacac catcgtcgtc gacaatgtat cagaactctt
     3061 tcgggcttgc ctagcgaatt tgggtcgcga gggcaagaac cacagggtgc cttctcaagc
     3121 ggactaccaa cgggtagatt tcactatttt ggatagtttg cgggccttat tgcagctaaa
     3181 aaaacgtatt gtgtttacag cttgggagac ttcggatcag tggacggatg aaaatggcat
     3241 gatttacaac agggccatgc ctgatattcg ttctaagatt ttgaataact tcttaggctt
     3301 gaccgatgtg gtggctagat tggtcaaaaa gaccaccgaa gacggcgagg aagtgagagg
     3361 ttttatctta caaccgtcgg ctagtgtcta cgcaaaaaat cgtctggatg accgtaaggg
     3421 gtgcaaagta gatgagcttt tcactacgga attaccaagt tgaactgatt ttggacatca
     3481 agaaatccat gcttgcaggt caccgtaaaa tcatggttca gtcaccgcca cggtctggaa
     3541 aaacagtttg catggcccac attgccaaga atgcaacaaa taagcaaaag acggtattgt
     3601 ttttcagtca tcgcaaggaa atcaacgaac aagtttttga gacgttcagt cgtgcaggtg
     3661 tcgacatggg attggtctat atcggcacag ttggcagtat cgtcaggaag ttaggaaaat
     3721 tacctatgcc aacgctcgtc ttggtagatg aagcgcatca tatcaaggct agtcaatacc
     3781 aacagatttt aaaatactat catcaggcgg ttcagttgtt ttttacaggt acgccaatac
     3841 gcttggatgg gtctggattt gacgatatgg cagatgattt ggttgttggt aaatctatct
     3901 tgtggttgca ggaacatgga aatatttcag agtttgatta ttattctatc aatttgcttg
     3961 atcaggcaaa actaaggaaa cggcagggcg agtacacgaa tcagtcgatt gatgatagtt
     4021 ttgatttcaa gcaacagcac ggcgattact tgagccatta cgaacgtttg gcaaagggaa
     4081 aacaagctat cgtatattgc catagcgtag aatacgctga gagggttttt aagcgatttt
     4141 ttgaagcagg gtaccaatca gccgtagtgt ctggaaaaac tccgaaagtc gaacgagagc
     4201 gggcaatgcg tgcctttcgt gacggagaag tgactatcat ggtgaacgtc aatttattta
     4261 ccgaaggaat tgacttgcca ggcgttgatg tctgcatcat gctacgaccg acggcatcgt
     4321 taagtttgta tttgcagttt gcaatgcgtg ccttgaatcc cagagatggc aaacgtgcca
     4381 tactaattga ccatgttgga aaccacattc ggcacggtct accaaacgat gaccgctact
     4441 ggaccttgga aggcgttgat aaaagaaaat catccagtaa agagaaggaa gaggcaccta
     4501 agacttgcga gaattgcttt gcgacatttt atagggatag gattgtcgat ggaaaatgtc
     4561 cttactgtgg tgaaccattg aaaattatca aggatattga aaaggaggca acaaatgaaa
     4621 cgttaacttt aatcaaccag gggatggagt ttgtctctat ccgtggtgag atgatagaag
     4681 tgacacggga agaggctttg gtctacaagc gtgtcaaacg atatggtaag aaatacgaga
     4741 gatgtgagtc tctcgcagaa ttaaaagcat ttagaattat ccacggttac gcccctggct
     4801 ggctgtggca caaacaaaaa gaattaaacc tttggaggaa ttaagaatgg gactttttac
     4861 agtaaattat gaagcagcag aacaattttc atctattgaa gacggaactt acgaagtatt
     4921 tatttcacat gtggagcaat ctgcaagtaa aggcggtaca gatttcttgg atattcgttt
     4981 gaaaatccgt gaggacttcc agcagaagtt ccgcaacaat cttatctttg acaagatttg
     5041 gatcaacaaa gaaaccttgc aataccctga gtttgctctt cagcgttatg ctaaggcggt
     5101 aaaattgcct gaaaacattg aaattcagac cgtggagcag tttttgaacc ttatcaaggg
     5161 taagaatttg aaagtcacgg ttaagaatga gcagtctgaa tacaacggca agacctatga
     5221 caacctgaat atcaagaaat acgagcaatc agaattgcca ccagttgctg tccaagctag
     5281 tcagcctgtt gtggatgatt tagatttacc gttctaagtt tatggcagga atggtagaat
     5341 acgccttgca ctatgctcgt cttggttttt ctgtcattcc gattgataag aggagcaaac
     5401 gtgccataac agcgtacaag gataagactt tttcagaatt ggaaatcaag cgtctgtggc
     5461 gtgataatcc agatgccaac atagctgtaa aaacaacgga tttctttgtt attgatattg
     5521 atgttcgaga tgatgtggat ggctattcca gttttgagga atgggaactg aagcaatata
     5581 tccctgccac tctacaggcg acaacgccga gtggtggcag gcatatattt ctcaagaaac
     5641 ctaaaggtgt tcaaattagt caggatatta aggttcgtcc agggatagat attaaggccc
     5701 acccgaacaa ttatgtcttg gtagcaccta gcaataatcc cagaggaaag tatgtctggg
     5761 atcagtctgt cgaagagatg gcagaggctc cgatagagct gttggacatc ttacaagcag
     5821 ggaagaaacc aagcaaaatc aattttacaa caaaatacaa cccagaatac agcagcaaaa
     5881 cggctaagct atttgaacaa attgttttcg gtctgggtga tgaaggtgga agaaacaata
     5941 atctagctag tttgattggc gggttattga tccgtggcgt tgatgaggaa gcggcttata
     6001 tgttagccaa aatagctaat cattatacgc caagtccttt atctcagcag gaggtggata
     6061 ggacatttga aagtatgtta agaaaggagt ttgataggcg aagtggtatt ggatatagcg
     6121 aagattaaag cagagtacga aaacgtcgtt ccacatccag ctgtctacga aaaaccgacc
     6181 gactggcgtg agattcgcct ggcttgtcgc gattaccgaa acgactggct ggaaaaagct
     6241 aagtggaaag aaacacagta tggaaccctg gagcagatta acgacgcacc gaaacgcttg
     6301 acagagttgg ctgtggcgga aggtttggag caaatcctgt atgtaatcaa tctaccgaac
     6361 gatagggtgg cagtttatga tcctgacgct ggttattatc ataaagaccc atcttttgct
     6421 tacaaggtta tcagattgtt agaacccact tttaccgaga cacggtccaa gaacgtactg
     6481 ttcatgttag cagcaaccaa acggaaatat ctatatgatg ggttctcgtg tgatttttct
     6541 atcggggatt accaggatcc gaaacgtttc attctggtga aaaacggtat ttttgacaaa
     6601 cagttgaaga agatgtcggg gtttacccat cggtttgtgg ctttctcgac tattgaaaca
     6661 gagtatgacc catttgcgga atcaccaaac attgacggtt gggatgtgga cgattggtta
     6721 ctggatttga tgagcggtga caaagatttg gttcatctct tatggcaggt tatttcagcc
     6781 agcctaaatg ggaactactc gtacaggaag tctatctggt ttgtcggtga aggaaatgac
     6841 ggtaagggta cggtccaaca attgattaca aatattgtcg gtattcgtaa tgtagcaacc
     6901 ttgaaattga atcagttttc ggagcgtttc gccttgtcaa tgattgaagg taaaactgtc
     6961 atcattgggg acgatgtgca ggctggtgtt tacattgacg aatcgtcgaa ctttaacagt
     7021 gttgtgactg gtgagccagt attagtcgag gagaagaaca agcaacctta ctcgaccgtg
     7081 ttcaagaaga ccgtcattca atcaaccaac gaattgccaa ggtttaaaaa taaaaccaac
     7141 ggaacctatc ggcgttttct tatcatcccc ttccgaaaga cattttcagc caaggaagat
     7201 aattggcaga ttaaggatga gtacatcaat agggatgatg tgaaacagta tgtgttgaaa
     7261 aaagcccttg agttaaactt cacacgattc agcgagccac aggcgacgct agatgtcttg
     7321 gaagaattca aatctagcaa cgatacagtt aaagcgttta tcgatgaatg gttcggaaca
     7381 tttcaatccg aacgcctgcc ggttcgtttc ttgtggtggt tgtatcagga gtggtgcaaa
     7441 gaagaaggga ttacaaaagt ggctaagggt aagtttgaac gtcagctcat caaattactc
     7501 cctgcagaat gggagaaaaa aagagcgaaa ccgacaagga gatttaagcc atcactagat
     7561 gtccctcgca gatatacagg tttttattgg gataacgata acgaccctaa cacgacagcg
     7621 gtctgtcttg ataaaaagtt actggttact gattaggtta ccgaactttt atagataggt
     7681 aacctagtta aacccttgat ataattgagt ttttgttaaa aaagttaccg gttacctatc
     7741 ttcccttatt atttattaaa ttataaatat aaaaaatata tataaataga aaataggggg
     7801 taacgggtaa cttttagggg taaaacaagg gctaaaccct tgatatgact ggttttttgg
     7861 aggttatcta tcttttttcg ag
//
LOCUS       DASFSW010000089         7788 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.089, whole genome shotgun sequence.
ACCESSION   DASFSW010000089 DASFSW010000000
VERSION     DASFSW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.089"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(21..1475)
                     /locus_tag="U1099_001502"
     CDS             complement(21..1475)
                     /locus_tag="U1099_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867817.1"
                     /translation="MGEKNSHHLPLDEEKVLDFEVAKDLTIEEAVKKHKEIEAGVTED
                     DGLLDRYIKQHRAEIESQKFETKINHLPLVEVADEEKNQGHESAEEVEANESSLTEVS
                     EEIAPIVEELSVAPMETLEETVIASTVAMEGLSSVADDSSLELEEDEAEDLDHSEGAD
                     RDQKKKFYFWSAVGLSMIGVMVTALIWMNSVNKSNTATSSSSTSTSQTSSTASSSTDA
                     NVTALEQLYNSFFTDSSLTKLKNSEFGKLAELKVLLEKLDKNSDSYTKAKEQYDHLEK
                     AIAAIQAINGQFDKEVVVNGEIDTTATVKSGESLSATTTGISAVDSLLASVVNFGRSQ
                     QEVASATVASEAAVTSSQGADETVSTGVPATTEVASTTVSGSTTDFGIAVPAGVVLQR
                     DRSRVPYNQAMIDDVNNEAWNFNPGILENIVTISQQRGYITGNQYILEKVNIINGNGY
                     YNMFKPDGTYLFSINCKTGYFVGNGAGHSDALDY"
     gene            complement(1485..2651)
                     /locus_tag="U1099_001503"
     CDS             complement(1485..2651)
                     /locus_tag="U1099_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEM4867818.1"
                     /translation="MKKQFELIATAAAGLEAVVGREIRNLGYECQVENGRVRFQGDVK
                     SIIETNIWLRSADRIKIIVGQFPAKTFEELFQGVFNLDWENYLPLGCKFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHFSRPEGVPLQEMGAEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRVEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAAMLAKN
                     IAPGLKRSFAFEEWPWVEDQLVAALRKEAQASIKTDLVLDIAGSDIDARMIEIAKKNA
                     FAAGVEQDIVFKQMRVQDLRSDKINGVIISNPPYGERLLDDEAIVTLYREMGQTFEPL
                     KTWSKFILTSDELFETRFGQQADKKRKLYNGTLKVDLYQFFGQRVKHQVQEVQG"
     gene            complement(2705..3069)
                     /gene="rnpB"
                     /locus_tag="U1099_001504"
     ncRNA           complement(2705..3069)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U1099_001504"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(3208..3543)
                     /gene="gpsB"
                     /locus_tag="U1099_001505"
     CDS             complement(3208..3543)
                     /gene="gpsB"
                     /locus_tag="U1099_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEM4867819.1"
                     /translation="MASIKFTTKDIFEQDFKIGFRGYDQDEVNDFLDDIMKDYDAYEA
                     IIKELKGEIARLKAQAANSPKTTLPTEESNDVLRTERPSSATNFDILRRLNRLEKEVF
                     GKQIVQDQE"
     gene            complement(3611..4150)
                     /locus_tag="U1099_001506"
     CDS             complement(3611..4150)
                     /locus_tag="U1099_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEM4867820.1"
                     /translation="MDTKSLLVTGYRHTDLGIFSEKDPRLHIIKSAIRRNFIRFLEEG
                     VSWFILTGQLGFEYWSLEVLEDLRAEGYQLSIATIFPFENHGEQWNEANQAKLARFKQ
                     VDFVKYAYPRYENPGQFRDYNQFLLDNTTGCYLFYDSENETNLKYLYHMVLKKEGYNR
                     KTLTFEELNEEAENFSNSE"
     gene            4216..4833
                     /gene="recU"
                     /locus_tag="U1099_001507"
     CDS             4216..4833
                     /gene="recU"
                     /locus_tag="U1099_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEM4867821.1"
                     /translation="MVNYPHKVSKKINRTSPISSQRVNFANRGMSFEAAINDSNQYYL
                     AHDIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVFKRHYIDFEAKET
                     RQKASMPMKNFHAHQIEHMKQVVKQGGICFVLLHFSTLKETYLLPATHLIEFYQVDMG
                     SKSMPLTFIRQYGFEIQMGRFPSIPYLEIVEKNLLGGESFENYNN"
     gene            4802..6988
                     /locus_tag="U1099_001508"
     CDS             4802..6988
                     /locus_tag="U1099_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEM4867822.1"
                     /translation="MVNLLKTTTIKKALLITANILLAGAIIACLAGAALVVYYIQSAP
                     ELTEESLTATVSSKIYDKNGNLIADLGAEKRSSAKTEEIPTDLVNAIVAIEDQRFFNH
                     RGVDVIRIAGSLINNLSGGRLQGGSTLDQQFIKLTYFSTSVEDQNLKRKIQEAWLATQ
                     LERRNTKQEILTYYINKVYMSNGNYGMKTAALAFYGKELKDLTLPQLALLAGMPQAPN
                     QYDPYTNPEDAKARRDLVLAEMLEEKYIDNPQYEQAVLTPVTDGLQPLSNAAAYPAYM
                     DNYLKQVVEEVEAKTGYNLLTTGMDVYTNVDPAAQQQLWNIYNTDMYVSYPDDLLQVA
                     STVVDVSNGKVVAQLGGRKQETNVSFGTNQSVETNRDFGSTMKPITDYAPAFENGIYT
                     STADLILDGPYNYPGTPTPVNNWDKQYYGNISVKTAIQYSRNVTAVKALEATGLENAL
                     KFLNSVGINYPDIHYSNAISSNTSDTSSKYGASSEKMAAAYAAFANGGTYYAPQYVNK
                     IVFSDGTVTEYAPKGTKVMSAETAYMMTDMMKAVMSYGYGLNASVSGVPMAGKTGTSN
                     YTDSETDTILASIPEANYSYMVVPDENFVGYSSQYAMAVWTGYTNRMTPILDNSMRIA
                     TDVYHNMMLFMHSDYTATDWEMPSGLVRYGSNYYLRGSRSLSNAYNTYTSSSTSSSSS
                     TETSETTEATTTSSETSSGTATETATSSTETATTGNNPATGQTDGQ"
     gene            7047..7760
                     /gene="rsmG"
                     /locus_tag="U1099_001509"
     CDS             7047..7760
                     /gene="rsmG"
                     /locus_tag="U1099_001509"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEM4867823.1"
                     /translation="MKPEVFYKTLADQGIQLTDQQKHQFHRYFQLLVEWNEKINLTAI
                     TEESEVYLKHFYDSIAPILQGHIQNEPLRLLDIGAGAGFPSLPMKIIFPQLDVTIIDS
                     LNKRINFLHLLAEELELEGVHFYHGRAEDFAQDKNFRAQFDLVTARAVARMQILSELT
                     IPYLKLNGKLIALKASSAEDELTQAKNALNLLFAKVIENHDYTLPNGDPRTLTIVEKK
                     KETPNKFPRKAGLPNKRPL"
BASE COUNT         2324 a         1760 c         1367 g         2337 t
ORIGIN      
        1 cggctaaaac gcttttttgc ttaataatcc aaggcatcag aatgaccagc accattgccg
       61 acaaagtagc ctgtcttaca atttatagag aacaagtagg tcccatctgg tttaaacata
      121 ttgtagtagc cattgccgtt gataatatta actttttcca agatatattg attgcctgtt
      181 atatacccac gttgttgcga gatagtcaca atattttcaa gaatacctgg attgaaattc
      241 catgcttcat tgtttacatc atcaatcatt gcttgattat aaggaacacg actgcggtca
      301 cgttgcaaga cgacaccagc tggcacggca ataccaaagt cggttgttga gcctgaaacc
      361 gttgttgaag cgacttctgt tgtcgctggt acaccagtag atacagtttc atcagcacct
      421 tggctgcttg tgactgcagc ctcacttgct accgtagccg aagcgacttc ctgttgtgaa
      481 cgaccaaaat tcacgaccga tgccaataga ctgtcaaccg cactaatccc tgtcgttgtc
      541 gcagaaagag attctcctga tttaaccgtc gccgttgtat caatttcacc attaacaacg
      601 acttctttat caaactgacc attaatggcc tgaatggctg caatggcttt ttcaagatga
      661 tcatactgtt ctttagcttt ggtataactg tccgaattct tatccaattt ttccaacagc
      721 actttcagtt ctgctaattt accaaactct gaatttttta gcttggtcaa actactatcc
      781 gtaaagaaac tattatagag ctgttctaaa gcggtcacat tcgcatctgt cgaacttgat
      841 gctgtcgatg acgtctggct tgtacttgtt gaacttgaag aagtcgcagt attcgatttg
      901 ttaaccgaat tcatccaaat cagcgccgtc accattacac caatcattga aagccctact
      961 gctgaccaga agtaaaattt ctttttctgg tccctatctg cgccctcact atggtccaag
     1021 tcttcggctt catcttcttc caattctaaa ctagaatcat ctgccactga agataacccc
     1081 tccattgcta cagtacttgc aatcactgtt tcttccaagg tttccatcgg agctaccgaa
     1141 agttcttcta caataggagc aatctcctca ctaacttccg taagagaaga ctcatttgct
     1201 tctacctctt ccgcgctttc atgtccttga tttttttcct catcagccac ctcaactaaa
     1261 ggaagatgat tgattttggt ttcaaatttt tgagattcaa tttctgcacg gtgttgcttg
     1321 atgtaacgat ccaagagacc atcatcttct gtcacacccg cttcaatttc cttatgtttt
     1381 ttgactgctt cttcgattgt taaatctttc gccacttcaa aatctaaaac tttctcctca
     1441 tccaaaggaa gatgatgact atttttttct cccacgatgt cctcctatcc ttgcacttct
     1501 tgcacttgat gtttcactcg ttgaccaaaa aattgataaa gatccacttt tagggtacca
     1561 ttatacagtt tgcgtttctt atccgcttgc tgtccaaaac gggtttcaaa caactcatca
     1621 ctagttagaa taaacttaga ccatgttttg agtggttcaa aagtctgccc catttctcga
     1681 taaagggtaa caatggcttc atcatccaac aaacgttctc cgtaaggtgg attagaaatg
     1741 atgacaccat taattttatc tgaacgcaag tcttgcacac gcatttgctt gaaaacaata
     1801 tcctgctcaa ctcctgccgc aaatgcattc ttcttcgcaa tttcaatcat acgagcatca
     1861 atatctgatc cagcaatatc taataccaaa tctgttttga tagaagcttg cgcttctttg
     1921 cgtaatgcag ccaccaattg atcctcaacc cacggccatt cctcaaatgc aaaacttctc
     1981 ttcaaacctg gagctatatt ttttgccaac atagctgcct caatacagaa agttcccgat
     2041 ccgcaagtag ggtcaataag cggtttatct ggataccaat tcgataattg caaaatagca
     2101 gccgccatat tttctttgat tggtgcgcca cctttttcca cccgataacc acgtttgaac
     2161 aaactagaac ctgtcgtatc aatcatgact gtcgccacat ctttcaaaat cgacacttca
     2221 attttaaact ctgcccccat ctcttgcaag ggaacccctt cgggacgaga gaagtgtttc
     2281 tgcagtttct ttacaactgc tttcttagaa atagcctgca cacttggttc attatgcaat
     2341 ttcgatttga cacatttggc cttggaaatg gggaacttgc agcctagagg caagtaattt
     2401 tcccaatcca agttaaaaac tccttggaac aactcttcaa aagtctttgc tggaaattgt
     2461 ccaacaatga ttttaattct atctgctgaa cgcagccaga tattggtttc gataatggat
     2521 tttacatctc cttggaatct aacacggcca ttttccactt ggcattcata gccgagatta
     2581 cgaatttctc gacccacaac cgcttccaga ccagctgctg ccgttgcaat caactcaaat
     2641 tgttttttca tttttttcct ctacaataaa agctagcaga gctagctacc tattttatgc
     2701 aattttctgt aagccatgtt ttgttccagt ggtactaggt tctaccacct tcgataatca
     2761 tctgtctact gtctaaacag tcaggatttc tgttcatttc catccatccc atgccccgac
     2821 caaagtttgg gttgctagct tgaggggttt accgcgttcc attttttacg tttccataaa
     2881 aacttcgtca ctgtggcact ttcagatcta ctctgacata tcaaaagact tagccatttt
     2941 aaccgccgta acgcccaagg gcgtccctag gcttattgat tcgcctagca caaacactac
     3001 tggcatctca gccagtgcta gcatggactt tcctcatggt agaaaactac cacgcgatta
     3061 tccaaaaatt gcaccttaat tatcttagaa gttgtatcta caaagcaagc cactttaaat
     3121 caaggaatat ctctgacaca aatacagaat gtatttgtgc acaagaaaat aactgcaaat
     3181 aaagtgtcag tttaggtgta caattttcta ttcttgatcc tgaacgattt gttttccaaa
     3241 tacttctttc tccagacgat tcagacggcg gagaatatca aagtttgtcg ctgaagatgg
     3301 acgttctgta cgcaatacat cgttcgattc ttcagtagga agagttgttt tgggggaatt
     3361 tgctgcctgc gctttcaatc gagcaatttc gccttttaat tccttgataa tcgcttcata
     3421 agcatcatag tctttcataa tatcatcaag aaaatcgttc acttcatctt gatcatagcc
     3481 acgaaaacca attttaaaat cttgctcgaa aatatctttt gtcgtaaatt taatacttgc
     3541 catctctctc cctaatctaa aacgtcacta ctcctattat attaaaaaaa cagggaaaaa
     3601 tcaagtcaaa tcactcagaa tttgaaaaat tttcagcctc ctcattcaat tcctcaaagg
     3661 taagcgtttt tctattatat ccttctttct ttaagaccat gtgatataaa tatttcaaat
     3721 tggtttcatt ttcagaatcg taaaataaat aacaacctgt cgtattatcc agtagaaact
     3781 ggttatagtc tctaaactgc cctgggtttt catagcgtgg ataggcatac tttacaaaat
     3841 caacctgctt aaaccgtgct aactttgctt ggttagcctc attccactgt tcgccatggt
     3901 tttcaaatgg aaatattgtt gcaatactca gttgatatcc ctcggctcgt aaatcttcta
     3961 agacttctaa ggaccagtat tcaaaaccca attgacctgt cagtataaac caactaactc
     4021 cctcttctaa aaaacgaata aaattcctac gaattgcaga tttaataatg tgcagtcgag
     4081 gatctttttc tgaaaaaatt cccaggtctg tatgtctata acctgttacc aaaaggctct
     4141 tagtatccat ttcttgtact cttttcgcta attatggtat aatatgctga tattatttta
     4201 tcaagaagga gttctatggt caattatcct cataaggtgt caaaaaaaat caatagaacc
     4261 agtcctattt catcccagcg agtaaacttt gctaatcgcg gaatgtcttt tgaggccgct
     4321 atcaacgata gcaatcaata ttaccttgcg cacgatattg ctgtcattca caagaagcca
     4381 actcccgtgc aaattgtaaa agttgattac cccaaacgga gccgtgctaa gattgtcgaa
     4441 gcatactttc gacaggcatc taccacagac tactctggtg tttttaaaag acattatatt
     4501 gattttgaag cgaaagagac acgccaaaaa gcatccatgc ccatgaaaaa ttttcatgcg
     4561 catcagattg aacacatgaa acaggttgtt aaacaaggtg gtatttgttt tgtccttctt
     4621 cacttttcaa cattaaaaga aacctatctc ttgccagcca ctcatttgat cgaattttat
     4681 caagtagaca tgggtagtaa gtcgatgcct ctaaccttta ttcgacaata cggctttgag
     4741 attcaaatgg ggcgtttccc cagcatccca tatctggaaa tcgttgaaaa aaatctatta
     4801 ggtggtgaat cttttgaaaa ctacaacaat taaaaaagct cttctaatca ctgcaaatat
     4861 tttacttgcg ggggctatca ttgcctgcct ggccggagct gcattggttg tttactacat
     4921 ccaaagcgct ccagaattga cagaagaatc tttgacagca actgtgtcaa gtaaaattta
     4981 cgataagaac ggaaatctta ttgcagattt aggagcagaa aaacgttcga gtgccaagac
     5041 tgaagaaatt ccaacagact tggtaaatgc gattgtcgca atcgaagacc aacgcttctt
     5101 caatcataga ggtgttgacg tgattcgtat tgcaggttcc ctgattaata acctgagtgg
     5161 tggacgtttg caaggtggct cgacacttga ccagcagttt atcaagctga cttatttctc
     5221 gacatccgta gaagatcaaa atctaaaacg gaaaattcaa gaggcttggc tagctactca
     5281 attggaacgt cgcaatacca aacaagagat tttgacctac tacatcaaca aggtgtatat
     5341 gtcaaatggt aattacggta tgaagactgc tgctcttgct ttttatggaa aagagttaaa
     5401 agatttgact cttcctcaat tagctctttt agcaggaatg ccccaagcgc caaaccaata
     5461 tgatccttat accaatccag aagatgcaaa agcacgtcgc gatttggtat tggctgagat
     5521 gctggaagaa aagtacattg ataatcccca gtatgaacaa gctgttctaa cacctgtaac
     5581 agatggtttg caaccactat ctaatgcagc tgcttatcct gcttatatgg acaactatct
     5641 gaaacaagtc gttgaagaag ttgaagcaaa aacaggttac aacttgctga caacagggat
     5701 ggatgtctat acgaacgtcg atcctgccgc ccagcagcaa ctttggaata tctacaatac
     5761 agatatgtat gtcagctatc cagatgattt attgcaggta gcttcaacgg ttgtggatgt
     5821 ttctaatggt aaagtcgttg cgcaattagg tggtcgtaaa caggaaacaa acgtctcatt
     5881 tggtaccaac caatccgttg aaaccaaccg cgacttcggt tctacaatga agccaatcac
     5941 agactatgct cctgcttttg aaaacggcat ttatacatct acagcagatt taatcttaga
     6001 cggtccttat aactatcctg gtacaccaac tcctgtcaat aactgggata aacaatacta
     6061 cggtaatatc tctgttaaga ctgccattca gtattctcga aatgttaccg ccgttaaagc
     6121 attggaagct acgggcttag aaaatgcctt gaaatttttg aatagcgttg gtatcaatta
     6181 tccagatatt cattactcta atgctatttc aagtaataca tccgatacta gcagtaagta
     6241 tggagcaagt agtgaaaaaa tggctgctgc ctacgctgct tttgccaatg gtggtactta
     6301 ctacgctcca cagtatgtca ataaaattgt ctttagtgat ggaacagtaa cagaatatgc
     6361 tcctaaaggg accaaagtca tgtctgccga aacagcctac atgatgaccg atatgatgaa
     6421 ggctgttatg tcttacggtt atggattaaa cgcttctgtt agtggtgttc ctatggccgg
     6481 taaaacaggt acatcaaact ataccgatag cgaaacggat actattctag cttcaattcc
     6541 agaagcaaat tatagttaca tggtcgttcc tgatgaaaac ttcgttggat attctagtca
     6601 gtacgcaatg gctgtttgga ccggatatac caatcgtatg actcctatcc ttgataacag
     6661 catgcgaatt gcaacggatg tctaccacaa tatgatgctc tttatgcatt cggactatac
     6721 agctaccgat tgggagatgc caagcggact cgtacgttac ggttccaact actatctcag
     6781 aggtagtcgc tcactctcca atgcttataa tacttacact agctctagta caagttcttc
     6841 tagttcgact gaaactagtg aaacaaccga agcgacgaca acatcaagcg aaacatcatc
     6901 tggaactgca accgagactg caacttcttc aactgaaact gcaacaacag gaaacaatcc
     6961 cgcaacggga cagacagatg gtcaataaga aaattcccag actcttctgg gtttttcttt
     7021 tgcttaaatt atgataagat aagattatga aacctgaagt attttataaa accttggcag
     7081 accaaggtat tcaattaact gaccaacaaa aacaccaatt tcatcgctat ttccaactat
     7141 tagtggaatg gaatgaaaaa atcaatctga cagctattac cgaggaaagc gaagtctatc
     7201 tgaagcattt ctacgattct atcgctccaa tcctgcaagg ccatattcag aacgaacctc
     7261 ttcggctttt agatattgga gctggagctg gattccctag cctacctatg aagattatct
     7321 ttccgcaact agatgttacc atcattgatt ctcttaataa acgaattaac tttttacatt
     7381 tgcttgccga agaactagag ctcgaaggtg ttcatttcta tcatggtcgt gcagaagact
     7441 ttgcacaaga taagaacttc cgtgcacaat ttgaccttgt cacagcacgc gcagttgccc
     7501 gcatgcagat tctttctgaa ttgactattc cttatttgaa gctcaacgga aaattaatcg
     7561 cactcaaggc gtccagcgca gaagatgaat tgacccaagc aaaaaatgca ctcaatctcc
     7621 tcttcgcaaa agtcatcgaa aaccacgact atactcttcc aaatggcgac cctcgaacat
     7681 tgaccatcgt cgaaaagaaa aaggaaactc caaataagtt tccacgcaaa gctggtctgc
     7741 caaataaaag acccctttaa acataagaaa agaggctggg caaaaagc
//
LOCUS       DASFSW010000090         7727 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.090, whole genome shotgun sequence.
ACCESSION   DASFSW010000090 DASFSW010000000
VERSION     DASFSW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.090"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(133..2127)
                     /gene="pknB"
                     /locus_tag="U1099_001510"
     CDS             complement(133..2127)
                     /gene="pknB"
                     /locus_tag="U1099_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEM4867824.1"
                     /translation="MIQIGKIFAGRYRIVRQIGRGGMADVYLARDLILDGEEVAVKVL
                     RTNYQTDQIAIQRFQREARAMAELDHPNIVRISDIGEEDGQQYLAMEYVNGLDLKRYI
                     KENAPLSNDVAVRIMGQILLAMRMAHTRGIVHRDLKPQNVLLTSNGVAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIILFEMLTGRIPYDGDSAV
                     TIALQHFQKPLPSVREENANVPQALENVVLKATAKKLNERYKSVAEMYADLASALSMD
                     RQNEPRVELEGNKVDTKTLPKLSQANVETKVPHTNSSAQVSATDKGSGKKEVAKSGNK
                     PVSKPRPGIRTRYKVLIGAILLTVIAAGLMFFNAPRTVTVPDVSGQTVEKATEMIEVA
                     GLEVGNITEEATATVDEGLVIRTSPAAKTTRRQGSKIDIVVATAALASIPDVVDKESD
                     TARQELEALGFQVTIKEEYSEKVAQGLVIKTDPGANSSAEKGAKITLYVSKGVAPQVV
                     PNVVGKSQENATQILQTAGFSIGTITQEYSSSVTAGQVISTDPVANTELAKGSIINLV
                     ISKGKELIMPDLTSGNYTYSQARSQLQALGVNAESIEKQEDRSYYSTTSDIVIGQYPA
                     AGATIDGTVTLYVSVASTRTSSDSSAGSSTSTSTSTGSGQ"
     gene            complement(2127..2864)
                     /locus_tag="U1099_001511"
     CDS             complement(2127..2864)
                     /locus_tag="U1099_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEM4867825.1"
                     /translation="MEIALLTDVGQKRSNNQDYINRYKNRAGIDLVVLADGMGGHRAG
                     HIASEMAATDLGAAWVDTQLVTLNDVREWMVAIIDAENQKIHELGQTEEYKGMGTTLE
                     AVVVIDNQMIYAHVGDSRVGLIRDGEYTRLTNDHSLVGALVRAGQLTEEEAQRHPQKN
                     IVTQSIGQAEPIEPDIALKTLEVGDYVLINSDGLTNMIPTEDIRDIVLSDVSIESKVE
                     TLVRFANNAGGLDNITVGLLHITEEAR"
     gene            complement(2902..4215)
                     /gene="rsmB"
                     /locus_tag="U1099_001512"
     CDS             complement(2902..4215)
                     /gene="rsmB"
                     /locus_tag="U1099_001512"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEM4867826.1"
                     /translation="MVINKHETARSLALSVLEQVFDQGAYSNIALNKALESSRLSAQD
                     KGLATELVYGTVSRKITLEWYLAHFIEDREKLDTWVYYLLMLSLYQLVYLDKLPTHAV
                     VNEAVNIAKRKPGTDKFVNAILRKLSQDSLPKPADIKRKNKRLSVQYSVPVWLVQTLI
                     AEYGDERAEKIFQSLHDRNKASVRVTDGQQVEQLAEELGAQHSQLSPVGLVKSHGHFA
                     GTDYFKKGLITIQDETSQLVAPTLGIQGDEIILDACAAPGGKTCHMASYLTDGKVYAL
                     DLYDHKLNLIEENARRLGLEDKIETKGLDASRVHEEFGPDTFDKILVDAPCSGIGLIR
                     RKPDIRYNKASMDFDSLKTIQLQILDSVCQSLKIGGIITYSTCTIIAKENQEVIQEFL
                     QNHPNFEQVTLEHPQTDIMVDGCLLITPEQYKTNGFFIGQLRRKS"
     gene            complement(4202..5140)
                     /locus_tag="U1099_001513"
     CDS             complement(4202..5140)
                     /locus_tag="U1099_001513"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEM4867827.1"
                     /translation="MTKLIFMGTPAFSAMVLRGLLADGNYNILAVVTQPDRAVGRKKV
                     IQMTPVKEVALEYNLPVYQPEKLSGSQEMDELMNLGADGIVTAAFGQFLPTKLLNSVD
                     FAVNVHASLLPKYRGGAPIHYALINGDERAGVTIMEMVKEMDAGDMISSDSIAIEESD
                     NVGTLFEKLAVVGRDLLLQTLPAYIAGDLKPVAQNPEQVTFSPNIQPEEEVLDWNKTA
                     RQLFNHIRGMYPWPVAHTYWQGERFKIQEAVEAEGEGPVGRVIARSKKELIIATGQGA
                     LSLKTVQPAGKPKMTIADFLNGAGRDIAVGDQFGDQ"
     gene            complement(5271..7661)
                     /locus_tag="U1099_001514"
     CDS             complement(5271..7661)
                     /locus_tag="U1099_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEM4867828.1"
                     /translation="MLAQIIVDVPLMQTDQAYSYRVPAELEEGLKVGVRVHVPFGKGN
                     RLIQGIVVDLIDEDDENNLQDLKELAEVLDYSPVLNQEQFWLADQMRKSVFSYKIALL
                     KSMLPSLLNSTYDKLLRPGLGLEMAEQISLFGDKEQIRFSDLDVTEQGKIMRLAQSGK
                     ILVDYVAKDKKQIKTEKWYRVCLEKLREADISNRAKKRQQLREFLLEHSEDQLLSNLK
                     SAYSADTLRYFQEKGYIEVWEEEVSRTQGVFDKVEKTQALDLNPEQAIAVREIVASIG
                     QESQTFLLQGVTGSGKTEVYLQVIDRVLKMGKTAIMLVPEISLTPQMTNRFISRFGQQ
                     VAILHSGLSDGEKYDEWRKIEAGNAQVVVGARSAIFAPLTNLGVIIIDEEHEATYKQD
                     SNPRYHARDVAKLRADYNRATLVLGSATPSLETRARASRGVYGRLTLNQRANPLARIP
                     EVEVVDFRDYIGQQEASNFTPVLIEKIREKLARKEQVVLMLNRRGYSSFVMCRDCGSV
                     DQCPNCDISLTLHMDTKTMNCHYCGFQKGIPQTCPNCQSRSIRYYGTGTQKAYDELQE
                     LLPEARILRMDVDTTKKKGAHEDLLERFGRGEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTALNLPDFRSSERTFQLLTQVAGRAGRADKEGEVLIQTYNPNHYAIAFAKEQDYEG
                     FYRYEMGIRKNLGYPPYYFTVGLTFSHKSEEFVVKKAYETVAFLRQHLTDAIQILGPT
                     PKPIARTHNLYHYQIILKYRHEDRLEEVLNQILDWTQERENQDLRLIIDNEPQNMM"
BASE COUNT         2060 a         1887 c         1478 g         2302 t
ORIGIN      
        1 tgagctttaa tttgatctag catgactttt ccccttttat ttttagtgta acataaaaaa
       61 actaacccat cacaaaatgt gataggttag ataacttaat gacattgggt atctccccaa
      121 ccttattcat ctttattgtc cgctacctgt tgaagtactt gtacttgtgg aacttcctgc
      181 tgaagaatcg ctactggttc tggttgatgc aacagagaca tacaaggtaa ctgttccatc
      241 aattgttgct ccagcagcag gatattgtcc aatgacgatg tcacttgtcg ttgaataata
      301 gcttctatct tcttgttttt caatactttc cgcattgaca cccaaggctt gtaattgact
      361 acgcgcctgt gaataggtgt aattgccaga tgttaaatca ggcataatca attcttttcc
      421 ttttgaaata actagattga tgatagaacc ttttgccaac tctgtattgg caacgggatc
      481 tgtgctgata acctgacctg cagtaacaga tgagctatat tcctgagtaa tggttccaat
      541 gctgaagcct gctgtctgaa gaatttgagt agcattctcc tgactttttc caacaacgtt
      601 tggaacaact tgtggcgcca ctcctttgga aacgtaaagg gtaatttttg cacccttctc
      661 agcagaacta tttgctcctg gatctgtttt gataacgagt ccttgggcaa ctttttcgct
      721 atattcttct tttattgtca cttggaaacc aagggcctcc aattcctgcc gcgcagtatc
      781 agactcctta tccaccacat ctggaataga tgccagcgca gccgttgcta ctacaatatc
      841 aatcttactc ccttgacgtc tggtcgtctt tgcagccgga gaggtccgta ttaccaagcc
      901 ttcatctaca gtcgctgtcg cttcttctgt gatatttcca acttccaatc ctgccacttc
      961 aatcatttct gtagcttttt caacggtttg accagataca tcagggactg taactgttcg
     1021 tggagcgttg aaaaacatga gtccagccgc tatgactgtt aacaaaatag ctcctatcaa
     1081 gactttgtag cgtgtacgta tgcctggcct tggttttgaa acaggcttat ttcccgactt
     1141 cgccacttct ttcttaccag accccttatc cgtcgcagaa acttgagcag aactgtttgt
     1201 atgcggtact ttcgtttcaa cattggcttg ggagagtttc ggcaaggttt ttgtatcaac
     1261 cttgttccct tctagttcaa ccctgggttc attctgacga tccatggata gagccgaagc
     1321 taaatctgca tacatttctg caaccgactt atagcgctcg tttaattttt ttgccgttgc
     1381 ttttagtaca acattttcta aggcctgagg tacgttggca ttctcttctc taacagatgg
     1441 gagaggtttt tggaaatgct gaagagcgat cgtcacagca ctgtctccat catagggaat
     1501 gcgacccgtc aacatttcaa aaaggatgat tcccatcgca taaatatcac tttgaatggt
     1561 cgctttcgaa ccacgcgcct gctcaggtga aagatagtga acagaaccca acatggagtt
     1621 ggtttgtgtc aaactcgttt cagcaaaggc aactgcaata ccaaagtctg ttactttagc
     1681 gactccattg gaagtcaaaa ggacgttctg aggtttcaaa tctcgatgga caattcctct
     1741 cgtgtgcgcc atgcgcatag caaggagaat ctgtcccata attcgcaccg caacatcgtt
     1801 ggaaagtggt gcattttctt ttatataacg ttttaaatcg aggccattga catattccat
     1861 agccagatac tgttgaccat cttcttcacc gatgtccgaa atacgaacga tattgggatg
     1921 gtcaagttct gccatggcac gcgcttcacg ttgaaaacgc tggatagcaa tttgatctgt
     1981 ctgatagttg gtccgaagaa ctttaacagc aacctcttct ccatctaaaa tcaaatctct
     2041 tgctaggtaa acatctgcca taccgcctcg accaatttgc cgaacgattc gataccgacc
     2101 ggcaaagatc ttaccgattt gaatcattac ctagcctcct ccgtaatgtg aagcaaacct
     2161 actgtaatat tatctagacc gcctgcatta ttggcaaaac gtacaagtgt ttctaccttg
     2221 ctttcaattg aaacatcact caatacaata tcacgaatgt cctcggttgg tatcatattg
     2281 gttaagccat cgctgttaat aaggacataa tctccaacct caagagtttt caaagcaatg
     2341 tctggttcaa ttggttcggc ttgtccaatg gactgcgtta caatattttt ttgaggatga
     2401 cgctgtgctt cttcttctgt gagctgacca gctcgaacaa gggcacctac cagcgaatgg
     2461 tcgttagtta gacgtgtata ttcgccatct ctaatcaatc caactcgtga atctccaaca
     2521 tgggcataga tcatttgatt gtcgatgaca acaactgcct caagagttgt tcccatcccc
     2581 ttgtattctt ctgtctgtcc aagttcatga atcttttggt tttcagcatc aatgattgca
     2641 accatccatt cacggacatc attcaaagtc actaattgag tatcaaccca agcagctccc
     2701 aaatctgttg ctgccatctc actagcaata tggccggcac ggtgaccacc cattccatca
     2761 gctaacacga ccaaatcaat ccctgcgcgg tttttgtagc gattgatata gtcttggttg
     2821 ttcgaacgtt tctgtcctac atcagtaagt aatgcaattt ccataatttt tatcgttgct
     2881 aagcaacttc ctcctcttat tctaggattt tcgtctaagt tgaccgatga aaaagccatt
     2941 tgtcttatac tgttcagggg ttatcaacaa gcagccatcc accataatat ctgtttgagg
     3001 gtgttctagt gtcacctgtt caaaattggg atggttttgc agaaattctt gaataacctc
     3061 ttggttttcc ttagcaataa tcgtacatgt gctataggtt attataccac caattttcaa
     3121 gctttggcaa acactgtcca gtatctgcaa ctggatagtc tttagagaat caaagtccat
     3181 cgatgccttg ttataacgaa tatctggctt acggcggatg agtccaatac ctgaacaagg
     3241 ggcgtccact aggattttat caaaggtatc tggaccaaat tcctcatgaa cacgactggc
     3301 gtccagacct ttggtctcaa tcttatcttc tagacctaaa cgacgtgcgt tttcttcaat
     3361 caggtttagt ttatgatcat ataaatccaa cgcataaact tttccatccg taagataact
     3421 tgccatatga catgtctttc cacccggagc tgcacaggca tccaagatga tttcatctcc
     3481 ttgaataccc aaagtcggag ctaccaattg actggtttca tcttgaatgg tgataagtcc
     3541 tttcttgaaa taatctgtcc ccgcaaagtg tccatgggat ttaaccaacc caactggtga
     3601 taactgtgag tgttgggcgc ctagctcctc tgccaattgc tccacttgtt gaccatctgt
     3661 cacgcgtacg ctggccttat tccgatcatg taagctttgg aaaatcttct ctgcacgttc
     3721 atcgccatat tctgcaatca gggtttggac caaccaaact ggcacagaat actgaacgga
     3781 tagacgtttg ttctttcttt taatatctgc tggtttcggc aacgaatcct gactaagctt
     3841 acgcaaaata gcgttgacaa acttatctgt cccaggcttt cgcttggcaa tattaactgc
     3901 ctcattgaca actgcgtggg taggcaattt gtccaaatat accaactggt agaggctcaa
     3961 catgaggaga tagtagaccc aagtgtccaa cttttcccta tcttcgataa aatgcgccaa
     4021 ataccactct agggtaattt tacgagaaac cgtcccataa accaattcgg tcgccaagcc
     4081 cttgtcctgt gctgataaac gagaagattc tagagcctta ttgagagcga tgttcgagta
     4141 agcaccttga tcaaagactt gttccaatac agataaggct agacttcttg ctgtttcatg
     4201 tttattgatc accaaattga tctcctactg caatatctcg acctgcacca tttaaaaagt
     4261 ctgcaatggt catttttggc ttaccagctg gctggacagt tttcagagaa agggctccct
     4321 gacctgtcgc aatgatcaat tctttcttgc ttcgagcaat aacccgacca actggtcctt
     4381 caccttctgc ttctacagct tcttgaattt taaaccgttc cccttgccaa taggtgtggg
     4441 caacgggcca cgggtacata ccgcgaatgt gattaaatag ttgacgagct gtcttattcc
     4501 aatccagtac ctcttcttct ggctggatat ttggtgagaa ggttacctgt tctggatttt
     4561 gagcgactgg tttcaagtca ccagcaatat aggctggtaa ggtttgtaat aataaatccc
     4621 gaccaacaac agccaacttt tcaaacaagg tgccgacgtt gtcgctttct tcaatagcaa
     4681 tgctatcgct ggaaatcatg tcaccggcat ccatttcctt gaccatttcc atgatggtta
     4741 cgccagcacg ttcatcaccg ttaatcaaag cgtaatggat aggggcaccg ccacggtatt
     4801 taggaagaag agaggcatga acattgactg caaagtcaac tgagttcaat aatttggttg
     4861 gtaggaattg cccaaaagct gccgtcacaa tcccatctgc tcctaaattc atcagctcat
     4921 ccatttcttg acttccagat agcttctctg gttgatagac tggtaaattg tattccaagg
     4981 ctacttcttt cacaggtgtc atttggatga ctttttttcg tccaacagct cggtctggct
     5041 gggttacaac tgccagtatg ttataatttc catcagctaa gagccctctt aagaccatcg
     5101 ctgaaaacgc tggcgttccc ataaaaatca actttgtcat attcgtgtct catctccttt
     5161 tcattttctg attcatatca attatactat cccctaatta tatcattttt gcttattttt
     5221 tccttaatca aaggttctta aaacaaaaaa atgcaaggtc aatagtgacc tcacatcata
     5281 ttctgcggtt cattgtcaat aatcagtcgc aaatcttgat tttctctctc ctgtgtccaa
     5341 tccaaaatct gattcaatac ctcttccaaa cggtcttcat gccggtattt caagatgatc
     5401 tggtagtggt agagattatg agttctggca ataggcttgg gcgtcggtcc caagatttga
     5461 attgcatctg ttaagtgttg acggagaaag gctacagttt cataggcttt tttcacgaca
     5521 aattcttctg acttgtgcga aaaggttaag ccaactgtaa aatagtaagg tggataacca
     5581 agatttttcc gaatgcccat ctcataacga tagaaacctt cataatcctg ctctttggca
     5641 aaagcaatgg cataatgatt ggggttatag gtctgaatca ggacctcccc ttccttatca
     5701 gctcgaccag cccgaccagc tacctgagtt agcaactgga aggtccgctc agaagaccga
     5761 aaatcaggca aattcagagc cgtatccgca ttgagcacgc caactagggt cacatttgga
     5821 aaatccaagc ccttggcaat catctgagtt cccagaagaa tatccgcttc gcctcgacca
     5881 aaccgttcga gcaagtcctc atgagcacct tttttcttgg tagtatccac atccatacgc
     5941 aaaattctag cttctggcaa gagctcctgc aattcatcgt aggccttttg cgtacccgtc
     6001 ccataataac gaatagaacg actttgacag tttggacagg tctgaggaat ccccttttga
     6061 aaaccacaat aatggcaatt catcgttttt gtatccatgt gaagcgtaag ggaaatatca
     6121 cagttgggac actggtcaac actcccgcaa tcacgacaca tgacaaaaga agaataacca
     6181 cgcctattca acataagaac gacctgttcc ttacgagcca atttttctct tatcttttca
     6241 atcagaacag gggtaaaatt actagcctcc tgctgaccga tataatcacg gaaatccaca
     6301 acttccactt ctggaatacg agccaaggga ttggccctct ggttaagagt cagtcttcca
     6361 tagactcctc gactagcccg agcccgtgtt tcaagactcg gcgtagcaga acccaagacc
     6421 agggtcgctc gattataatc agcccgcaac tttgccacat cacgcgcatg gtagcgtgga
     6481 ttgctgtcct gcttgtaggt cgcctcatgc tcctcatcaa tgatgataac acccaagttt
     6541 gtcaatggtg caaaaatagc cgaacgagcc ccgacaacca cctgggcatt ccccgcctca
     6601 atcttccgcc actcgtcata cttctcacca tcagacaagc ccgagtgtaa aatggcaacc
     6661 tgctggccaa aacgagaaat aaaacgattg gtcatctgag gggtcaagga aatttcaggc
     6721 actagcataa tagccgtttt tcccattttc aagaccctat caataacctg gagatagact
     6781 tccgtcttcc cagaacctgt cactccttgc agtagaaatg tctgactttc ctgaccgata
     6841 gaggccacaa tctcacgaac cgcaatcgcc tgctctggat tcaaatccaa ggcctgcgtt
     6901 ttttccacct tatcaaaaac tccctgagtc cgcgacactt cctcctccca gacttctata
     6961 tagccttttt cttggaaata gcggagcgta tcagctgaat aggccgattt taggtttgaa
     7021 agaagctggt cttccgagtg ttccagaaga aattccctta actgctgccg tttcttggct
     7081 cgattgctga tgtctgcttc tcgtaatttc tccagacaaa ctcggtacca tttttctgtc
     7141 ttgatttgct tcttatcctt ggccacatag tctaccaaga tttttcctga ctgggccaga
     7201 cgcatgattt tcccttgctc tgtcacgtcc aagtcagaaa aacgaatctg ctccttatca
     7261 ccaaacagac taatctgctc tgccatttcc aaaccaagcc ctggacggag caatttgtca
     7321 taggtcgaat tgagcaagct aggcaacatg gattttaaga gggcaatttt ataggagaag
     7381 accgactttc tcatctggtc cgccaaccaa aattgctctt gattgagaac aggactatag
     7441 tccaaaacct cggcaagctc tttcaagtct tgtaagttat tttcatcatc ttcatcaatc
     7501 aaatcaacta caataccttg aatcagccga tttcccttac caaagggtac atggacacgc
     7561 acgccaacct tgagcccctc ttccaactca gctggaactc gataggaata agcttgatct
     7621 gtctgcatca aagggacgtc tacaataatc tgtgctaaca taccatctcc atttatttta
     7681 ccttctaata tcacgactga tgcgaacaag gttcacttta tgtataa
//
LOCUS       DASFSW010000091         7724 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.091, whole genome shotgun sequence.
ACCESSION   DASFSW010000091 DASFSW010000000
VERSION     DASFSW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.091"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(17..514)
                     /locus_tag="U1099_001515"
     CDS             complement(17..514)
                     /locus_tag="U1099_001515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867829.1"
                     /translation="MILLTLYGMLILAAFWLWLFIARTSVKLACFAIDFCAVFIYTIY
                     LLHQPISSKIANGNTIYFWDVLFGLIAVLIYGFLMVVLSILLPRISKAINFVIVFLGV
                     GIGICLATDFITFLLSIFHSSIEPTYRIQFLTNNLANDILYYVIFFLVSIPVWKKRME
                     YLTGL"
     gene            680..1372
                     /locus_tag="U1099_001516"
     CDS             680..1372
                     /locus_tag="U1099_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867830.1"
                     /translation="MFSPKRLKEKRTELGLSQSEIARQLEISRMSYSAWERGKTKPNH
                     KNLSLLATILKVDESYFESEYSIVQNYLLLTDENKLAAEDYVEQLLTSQSIAENVVSL
                     YSYQVLSDIQLSAGPGEGLYDEFETETVYSEDEYTGFDIATWISGNSMEPVYKDGEVA
                     LIRSTGFDYDGAVYALNWNGSLYIKKLYREEDGFRMVSLNEDYTEKFIPYEDEPRIVG
                     LVVGHFMPVIGG"
     gene            1515..2930
                     /locus_tag="U1099_001517"
     CDS             1515..2930
                     /locus_tag="U1099_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM4867831.1"
                     /translation="MSYIDYSKEPRSDIAFVDMKSFYASIECVERGLHPLHTSLCVMS
                     RADNTSGLILASSPMFKKIFGKGNVGRAYDLPFDVHTRKFNYYRASISGLPTDVKFVS
                     YIENWAKRTFIVPPRMDLYIQKNLEIQKVFQNYADPTDILPYSIDEGFIDLTSSLNYF
                     VEDKTLSRKDKLDLVSAKIQHDIWEKTGVYSTVGMSNANPLLAKLALDNEAKSTATMR
                     ANWSYEDVETRVWNIPKMTDFWGIGSRTEKRLNKLGIYTIKELANCDPSILKKEFGVI
                     GVQHWFHANGIDESNVHEPYRPKAVGIGNSQVLHKDYTRQSDIELVLREMAEQVAIRL
                     RKRHKKATVVAINVGYSNFENKKSINVQRKINPTNRTLVFQDEVISLFRSKYDGGAVR
                     SIAVRYDGLVDENFAVISLFDDFEESEKEEKLETTIDTIRDRFGFLAVQKASSLLENS
                     RAIARSKLVGGHSAGGLEGLK"
     gene            2927..3277
                     /locus_tag="U1099_001518"
     CDS             2927..3277
                     /locus_tag="U1099_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867832.1"
                     /translation="MIDRSYLPFQVAREYQDRKMAKWMGFFLSEHTSGLEQERNRIDY
                     STELTLADKLLLLNQLYSHELNGILTVPGKIYIGIVKNLTFQSVSFETKEGYISIQIS
                     EILTIDLEVEYESA"
     gene            3264..3557
                     /locus_tag="U1099_001519"
     CDS             3264..3557
                     /locus_tag="U1099_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867833.1"
                     /translation="MNQLEFNKNQLQMDYFSDSYRKFEEDFYRYSKLAVPLTFLTDDL
                     MNSMASSGINYFVLNKEKSKDNRDHYFIFKIETYSDNKLIRKFIYQKTTTSLE"
     gene            complement(3604..4242)
                     /locus_tag="U1099_001520"
     CDS             complement(3604..4242)
                     /locus_tag="U1099_001520"
                     /inference="COORDINATES: protein motif:HMM:NF012651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM4867834.1"
                     /translation="MNFEEMKSTLENMLNENYADFTKAIIAIEKDISDPDLLQQVYQA
                     YMDDDSISGLLHSNIDAIIETLESDKSQLSTDNVELVGNVTKDVEVKVFPGEDGKEFK
                     VANFSIARNDEQGNSHFTNVTAYNGRADFADQLKKGDFVKLTGQMKYEEYEGKLYKNL
                     VVKTIKILKTKEKQLEEAGKGEKPSTLGALKEYSQHTSSEPSKPKQDKGMEL"
     gene            complement(4239..>7724)
                     /locus_tag="U1099_001521"
     CDS             complement(4239..>7724)
                     /locus_tag="U1099_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM4867835.1"
                     /translation="ISVRLGATWIPTSDIEQFIFDTLQPSYYAKKAIKVQFSPYTSEW
                     KIDGKTQDSRNDLANLTFGTSRVNAYRLIEDSLNLRDTRVYDTVYDDAGNKKQVLNKK
                     ETMLAGEKQELLKESFKDWIFKDQSRRHRLEKLYNEKFNSIRNREYDGQHLSLEGMTS
                     DISLRPHQKNAIARTLYGGNTLLAHVVGAGKTFEMVASAMESKRLGMSNKALFVVPNH
                     LTEQMGREFMELYPAANIMVATKKDFEPANRKRFVGRIATGEYDAVIIGHTQFEKIPM
                     SREYQEQHLKHQIQDIVEFIEEYKYERDQNFTVKQLEGTKKKLEAKLKKLNDDFRKDD
                     VVTFEELGVDKLYIDEAHNYKNLYLYTKMRNVAGIGQSEAMKSSDMFMKCRYLDELTG
                     GKGVVFATGTPVSNSMSELYTMQRYLQFDELERKGLHHFDSWASTFGETVTAIELSPE
                     GDSYRSKTRFSKFYNLPELMSLVKEMADIKTADQLNLPTPQAHYETILTKPTGHQKEI
                     LKSFSERADKVRNRKVEPQEDNMLRITNDGKKIALDQRLVNSFLPDDPESKVNVCTKN
                     IFAIWEQSSERRSAQLVFCDMSTPKGDGSFNLYDDMKTKLVNLGIPENEIAFIHDANN
                     EKQKAELFAKVRSGDIRILFGSTSKMGAGTNVQSKLIALHDLDVPWRPADLEQRSGRI
                     VRQGNENKDVYIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVADDVDENTLN
                     YAEIKALATGNPLIKEKMDLDVEVSKLRMLESNYKSNLYQLEDKIAKHYPFEIARLTE
                     QIAFAKQDMERKVSKVQGDGQFNGMEILGKHFVDKKSAAEELMKILPLVPASGTMSIG
                     HYRGFEVSAFFNQIAQAMDFVIEGNLSYVGHFGDSPEGNIQRIDNVLDRIDEEVSKLE
                     NRLSEVQYKLQVAKEEVTKPFEKADLLKEKTLRLAEVNRILDMGDVEELENPNPLLED
                     VKAAIVDFLAREYGDTELSEESRTEQFKDIFSTGDVVYLAKGKSEVGDYDIEYSVELS
                     NFSWKLSFADSDIMSGQFEGSTPEEQAQHFKLFLEHSTNEDLLSIDEKEACQTLGLPY
                     TRDLLDKDLDNDGIVDRYDHDFKDSDAFESNYDVDEKEKRPSTLAQIQAYKAEVEERD
                     YKSQTKEEIEEEQVI"
BASE COUNT         2354 a         1539 c         1346 g         2485 t
ORIGIN      
        1 atcgaatcat ttaattctat agcccagtta aatactccat tcgctttttc cagacaggaa
       61 ttgaaactaa gaaaaagatt acatagtata aaatatcatt ggcaagatta ttagtcaaaa
      121 attgtatgcg ataggtcggc tcaatactcg aatgaaagat tgataataag aaagttatga
      181 aatcagttgc taaacaaatt cctataccaa cacccaaaaa tacaatgaca aaattgatgg
      241 ctttacttat tcttggcaat aatattgaga gaacaaccat tagaaaacca tatatcaaaa
      301 ctgcgatcaa tccaaataaa acatcccaaa aataaatcgt atttccattc gcaatcttac
      361 tagaaattgg ttggtgtaac agataaattg tgtagataaa tactgcacaa aaatcaatcg
      421 caaaacaggc aagttttaca gaagttctag ctataaataa ccaaagccaa aatgctgcta
      481 agattaacat tccatacagt gttaacaaaa tcattaaaaa ctcactcctt tcactacatc
      541 tagtttactg cattatacta tttttgtccg ctcattatat tgaatttctg acgaaatttt
      601 taaagttata cttgtcaaat taaaaaaaaa taatttatac tttttgtaag tttataaact
      661 tacaacgaaa ggagtaacca tgttctctcc aaaaagacta aaagagaaaa gaacggaact
      721 aggactatct caatctgaaa tcgcacgaca acttgaaatt agtcgcatgt catatagtgc
      781 ctgggaacgt ggaaaaacca aaccaaacca taaaaatctt tctctgcttg ctactatttt
      841 gaaagttgac gagtcctatt ttgaatctga atattctatc gttcaaaact atcttttgtt
      901 aaccgatgaa aacaaattgg ctgctgaaga ttacgttgag caactactaa catctcaatc
      961 tattgcagaa aatgttgtgt cactatactc ctatcaagtg ctttcagata tccaattatc
     1021 tgctggtcct ggtgaagggc tgtacgatga atttgaaaca gagactgtct attcggaaga
     1081 tgaatatacg ggctttgata tagcgacatg gatttcagga aactcaatgg aacctgttta
     1141 taaagacggc gaagtcgcct taattaggtc tactggtttt gattatgatg gtgctgtcta
     1201 tgccttaaat tggaatggct cactctacat aaagaaactc tatcgtgaag aagatgggtt
     1261 cagaatggtc tccctgaatg aagactatac agagaagttc atcccttatg aagatgagcc
     1321 aagaattgtt ggtctagttg tgggccactt tatgcctgtg attggaggtt agcaatggca
     1381 agacagtttg acgatatgat ttacattaaa ggaaatcgca ttattctagc actcgaccaa
     1441 aaagattacg atgtaacaga ccatctaaca gagcttgttg aagagttagc aaaattgaaa
     1501 tctaggtgaa cactatgagc tatatcgact attcaaagga accacgtagc gatattgctt
     1561 ttgtcgatat gaaatctttc tatgcgtcca ttgaatgcgt ggagagaggg cttcatccgc
     1621 tccatacatc tctttgtgtt atgagtaggg ctgataatac ttctggcctc atactagctt
     1681 cttctccaat gttcaaaaaa atttttggta aaggaaatgt cggtagagct tacgacctcc
     1741 cctttgatgt ccacacacga aaatttaact attaccgtgc aagcatttct ggactaccaa
     1801 cagatgtcaa gttcgtctca tatattgaaa actgggctaa gcgtaccttt atcgtgccac
     1861 caagaatgga cttgtatatc caaaaaaatc tagaaatcca aaaagtattt caaaattatg
     1921 ctgatccaac agatattctc ccctattcaa ttgacgaagg atttattgac ctaacttcat
     1981 cactaaatta ttttgttgaa gataagacgc ttagtcgtaa agataaatta gatctagtat
     2041 cagcaaaaat acagcacgat atttgggaaa agactggagt atattctaca gttggtatga
     2101 gtaatgccaa tccacttcta gccaagctgg cacttgacaa tgaagcaaaa agtactgcta
     2161 ccatgcgagc aaattggtcc tatgaagatg ttgaaacaag ggtctggaac attccgaaaa
     2221 tgactgactt ttggggcatt ggttcacgaa cagaaaaaag attgaataaa cttggtatct
     2281 atactatcaa ggaattggcc aactgtgacc catctatctt gaaaaaagaa tttggtgtga
     2341 ttggagtaca acattggttt catgctaatg gtattgatga aagcaatgta cacgaaccct
     2401 atcgccctaa agctgtcggg attggaaatt cgcaagtgct ccacaaagat tacactcgtc
     2461 aaagcgatat agaacttgta cttagagaga tggcagagca agttgccatt cgcttacgta
     2521 agagacacaa aaaagcaacc gtagttgcca taaatgttgg atattcaaat tttgaaaaca
     2581 agaaatccat caatgttcag agaaaaataa atccaaccaa tcgaacactt gtttttcagg
     2641 acgaagttat ttcacttttt cgctcaaaat atgatggtgg tgcagttaga agtattgctg
     2701 tgagatacga tggattagtg gacgaaaact ttgcagttat ttcacttttt gacgactttg
     2761 aagaaagcga aaaagaggaa aaattggaaa caactattga taccattcgt gatcgatttg
     2821 gatttcttgc agtacagaaa gctagttctt tattagaaaa ttcaagggca attgctcgaa
     2881 gtaaacttgt cggcggtcat tccgctggcg gattggaggg attaaaatga ttgaccgtag
     2941 ctatctacca ttccaagtag ctagagaata ccaagataga aaaatggcaa aatggatggg
     3001 atttttcttg tcagagcata cttcaggttt agaacaagaa cgcaatcgta ttgattattc
     3061 aactgaattg acattagctg ataaactact cctactcaat caactatata gtcatgagtt
     3121 aaatggtatt ctgacagttc ctggaaaaat ttacattgga atagtaaaaa atttgacatt
     3181 ccaatctgtt tcgtttgaaa ctaaagaagg ctacatcagt attcaaatct cggaaattct
     3241 tacaatagat ttggaggtgg agtatgaatc agcttgagtt taataaaaat caattacaaa
     3301 tggattattt tagtgatagc taccgaaagt ttgaagagga tttctatcgc tactcaaaat
     3361 tagccgttcc acttaccttt cttacagatg atttgatgaa ttcaatggct tcatcaggta
     3421 ttaactattt tgttttaaac aaagaaaaat caaaagacaa ccgagatcat tatttcattt
     3481 ttaaaattga aacgtatagt gataataagt taattcgtaa gttcatatat cagaaaacga
     3541 caacaagttt agagtaaata aaaaaacaag ctagacctcc gaaaatggtg gtctagcttt
     3601 ttgtcacaac tccatccctt tatcctgttt tggtttactt ggttctgaac ttgtatgttg
     3661 ggagtattcc ttcaaagctc cgagagttga gggcttttct ccttttcctg cctcctctag
     3721 ttgtttttct tttgttttaa ggattttaat agttttaaca acaagatttt tgtagagttt
     3781 cccttcatac tcttcatact tcatctggcc agttagcttg acaaaatccc ctttcttgag
     3841 ttggtctgca aagtcggcac gtccgttata agctgtcaca tttgtaaagt gactattccc
     3901 ttgttcatca tttcgagcaa ttgaaaaatt ggcaacttta aattcctttc catcttctcc
     3961 agggaagact ttaacctcga catctttagt tacattgcca accaattcta cattatctgt
     4021 tgataattga cttttatcgg actccaaagt ttcaattatt gcatcaatat tggaatgaag
     4081 taaaccagat atagaatcat catccatgta tgcttgatag acttgctgca acaagtctgg
     4141 atctgaaata tctttctcaa tcgcaataat ggccttagta aagtccgcat aattctcatt
     4201 taacatattc tctaaagtag atttcatttc ttcaaaattc atattacttg ctcctcttct
     4261 atttcttctt ttgtttggct tttgtagtca cgttcttcaa cttctgcctt ataggcttga
     4321 atttgtgcta aagtagatgg ccgtttttct ttctcgtcga catcatagtt tgattcaaaa
     4381 gcatcactat ctttaaaatc atggtcatat cggtctacga taccatcatt atccaaatcc
     4441 ttgtcaagca aatctctagt ataaggtaaa cctagcgttt gacaagcctc cttttcatca
     4501 attgagagta agtcctcatt ggtggaatgc tccaaaaata gtttaaagtg ttgagcttgc
     4561 tcctctggtg tacttccttc aaactggcct gacattatat ctgaatccgc aaaggataat
     4621 ttccaggaaa aattggacag ttcaacacta tactcaatat catagtctcc aacctcgctt
     4681 tttccctttg ctagatacac cacatctcca gtcgaaaaga tgtctttaaa ttgttccgtt
     4741 ctactttctt cagaaagttc tgtatctcca tattctctgg ctaagaagtc aacaattgct
     4801 gccttaacat cttcaagtaa aggattcgga ttctctaact cttccacatc ccccatatcc
     4861 aggatgcgat tgacctctgc taaacgcaaa gtcttttctt ttagtaagtc cgccttttca
     4921 aaaggtttcg ttacctcttc tttcgcaacc tggagcttgt attggacctc tgacagtcta
     4981 ttttccaact tggatacttc ttcatctatc ctatccaaaa cattgtctat acgttgaata
     5041 ttgccctcag ggctatcacc gaaatgacca acgtaggata aattaccctc aatcacaaaa
     5101 tccattgctt gagcaatttg gttaaagaat gcggaaactt caaatccacg atagtgacca
     5161 attgacattg tacctgatgc aggaactagt ggtaaaattt tcattaattc ttctgctgct
     5221 gatttcttat ctacaaagtg ttttcctaaa atttccatac cattaaattg accgtcacct
     5281 tgtacttttg aaacctttct ctccatatct tgcttggcaa atgcaatttg ttctgttaga
     5341 cgagcgattt cgaacggata gtgttttgct atcttatctt ctaactgata taaattggac
     5401 ttgtagtttg attccagcat ccgtaattta gatacttcaa catctaaatc catcttctct
     5461 ttgataagtg gattaccagt cgcaagtgcc ttgatttccg cataatttaa ggtattctca
     5521 tccacatcat cagccacacg cacaggcgtt ttcgaggtca taatttgaga gataaacttc
     5581 tgtttattct caatggtctg ccaaagatat gcgtcgaatg tattctccgt cacataacga
     5641 tagatataga cgtccttatt ctcatttccc tgacgaacga tccgaccaga ccgttgctcc
     5701 aaatctgctg gtctccaggg tacatccaag tcgtggaggg caatgagttt gctttgcacg
     5761 tttgtacctg cccccatctt cgatgtggaa ccaaaaagaa tacgaatatc gccacttctc
     5821 accttggcaa acaactcagc tttctgcttt tcattattcg catcatgaat aaaggcgatt
     5881 tcattttctg gaattccaag attaactaac ttggttttca tatcatcgta gaggttaaaa
     5941 cttccatccc ccttcggtgt agacatgtca caaaatacga gctgagcgga acgtcgctca
     6001 cttgattgtt cccagattgc gaaaatgttc ttggtacaga catttacctt gctctctgga
     6061 tcatctggta aaaaggaatt aaccaaacgc tggtctaaag caatcttctt accatcattc
     6121 gtaatccgca acatattatc ttcctggggc tcgactttcc tatttctcac cttatcagca
     6181 cgctcggaaa agctttttaa aatttctttc tgatgacctg ttggcttggt aagtatggtt
     6241 tcataatgag cctgtggagt tggtaaattc aactgatctg cagtcttaat atctgccatc
     6301 tcttttacaa gagacatcag ttcaggaaga ttgtaaaact ttgagaagcg tgtcttacta
     6361 cgataggagt ctccttcagg agaaagttct atggcagtta cggtttcgcc aaaggtcgaa
     6421 gcccaggaat caaaatgatg tagccctttt cgttccagtt catcaaattg tagatatcgt
     6481 tgcatggtgt aaagttcgct catagaattc gacacaggtg ttcctgtcgc aaaaacgaca
     6541 ccctttcctc ctgttaattc atccaaatag cgacacttca taaacatatc cgaagatttc
     6601 atggcttcag attgaccaat cccagccaca tttctcattt tggtgtaaag ataaagattc
     6661 ttatagttat gggcttcatc aatgtagagt ttatcaacac ccaattcctc gaaagtcaca
     6721 acatcatcct tacgaaaatc gtcatttagt ttttttagct ttgcctctag tttcttcttc
     6781 gtcccctcaa gctgttttac agtaaagttt tggtctctct catacttata ttcttcaatg
     6841 aactcgacta tatcctgaat ttgatgtttc agatgttgtt cctggtattc tctactcatt
     6901 ggaatcttct cgaattgagt gtgaccaata atgaccgcgt cgtattcgcc agtcgctatc
     6961 ctacctacaa accgttttcg attagcaggt tcaaaatctt ttttagtggc taccataata
     7021 ttggctgctg gatacaactc cataaattcc cttcccatct gttctgtcaa atggtttgga
     7081 acaacaaaca gggctttatt actcatccca agtcgcttac tttccatagc acttgctacc
     7141 atctcaaacg ttttacctgc tccgactaca tgagctaaaa gggtatttcc tccataaagc
     7201 gttcgtgcta tggcattctt ttggtgtgga cgtaggctaa tatctgatgt catcccctca
     7261 agagagaggt gttggccatc atattctcgg tttcggattg agttaaattt ttcattgtag
     7321 agtttttcaa gcctatgcct acgactttgg tctttgaaaa tccagtcttt gaaagattct
     7381 ttgagaagtt cctgcttttc accggccaac atggtttctt tcttgttcag gacttgtttt
     7441 ttatttcctg catcatcata aactgtatcg tagactcgtg tatctcgtaa atttagtgaa
     7501 tcctcgatta accgataggc attgactcga cttgtaccaa aagtcagatt ggcaaggtca
     7561 tttcgactat cctgtgtttt cccatcaatc ttccattccg atgtataggg agagaactgt
     7621 actttaattg cttttttcgc atagtagcta ggttgaagcg tatcaaagat aaactgctca
     7681 atatctgaag ttggaatcca ggtagcccct agtcggactg aaat
//
LOCUS       DASFSW010000092         7646 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.092, whole genome shotgun sequence.
ACCESSION   DASFSW010000092 DASFSW010000000
VERSION     DASFSW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.092"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(196..1863)
                     /gene="dnaX"
                     /locus_tag="U1099_001522"
     CDS             complement(196..1863)
                     /gene="dnaX"
                     /locus_tag="U1099_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEM4867836.1"
                     /translation="MYQALYRKYRSQTFGEMVGQEVVATTLKQAIEQGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVGGEPCNDCYICQAITEGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSLATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKSIKVTDIQEHLAAILTKEGLIFDEQALTIIARRAEGGMRDALS
                     ILDQALSLSQDQQVTLEIAEEITGSISLRALDDYVASLRQGDSVTALQHLQTLFDQGK
                     SMSRFATDLLHYLRDILIVQTGGEDTHLTTGFSENLALEQARIFTMIEMVTRGLADIK
                     SSPQPKIYAEMMTIRLAEDSSPSGAMAELPADLLGQLASLQQQVADLQKQLGQLSSQP
                     SAVKPVSRKPQAPKKYRLDTSKVHAILQEAMENPTLARENLTRLQNAWGEIIASLSGA
                     DRALLVGSQPVAANENHAILAFDSPLNAEQTMKRDNLNTMFGNILSKAAGFSPQILAI
                     AQEDWVSIRAEFSAKAKGQKTQEIEKEASPVPEEFGFLADTVEIRND"
     gene            complement(1863..2360)
                     /locus_tag="U1099_001523"
     CDS             complement(1863..2360)
                     /locus_tag="U1099_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEM4867837.1"
                     /translation="MTKQEKISNYQLLLAQLEALLEGETNALANLSNASALLNQALPN
                     SVFTGFYLYDGSELILGPFQGGVSCVHIALGKGVCGEAAAKRQTILVDDVRLHDNYIS
                     CDATALSEIVVPMVKNDQLLGVLDLDSRLVADYDTIDQDYLEKFVALLVEKTDWNFAM
                     FGEKR"
     gene            complement(2425..2694)
                     /locus_tag="U1099_001524"
     CDS             complement(2425..2694)
                     /locus_tag="U1099_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEM4867838.1"
                     /translation="MSIKQMVSMALVVWNLVVFITYGMDKGKARKNAYRISEKTLLLM
                     SYFGGGLGAWAGGTHFRHKTQKKYFQLAWAIGVLIDAVIMYWMWK"
     gene            complement(2782..3320)
                     /locus_tag="U1099_001525"
                     /pseudo
     CDS             complement(2782..3320)
                     /locus_tag="U1099_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
     gene            complement(3322..3951)
                     /gene="udk"
                     /locus_tag="U1099_001526"
     CDS             complement(3322..3951)
                     /gene="udk"
                     /locus_tag="U1099_001526"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEM4867839.1"
                     /translation="MTQKPIIIGVTGGSGGGKTSVSRAILDNFPNARISMIEHDSYYK
                     NQSHLTFEERILTNYDHPLAFDTDLMIYHISELLAGRSVDIPIYDYTQHTRSEKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDIRIIRRIKRDMEERGRSLDSIIEQ
                     YTSVVKPMYHQFIEPTKRYADIVIPEGVSNVVAIDLINTKVESILRERG"
     gene            4034..5020
                     /locus_tag="U1099_001527"
     CDS             4034..5020
                     /locus_tag="U1099_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM4867840.1"
                     /translation="MLNFGIVGTSDISHRFIEAAHLSGHYQLQAVFSRKLETAAAFSE
                     QYEGVDLYTEWVKFLSAPIDVVYIASPNALHFEQAKAVLAAGKHAIVEKPMVSTPQEL
                     EQLRRVAQENNVFLFEAARNYHEQAQAIIREFLTDKTILGGSFGYAKYSSKMPELLAG
                     QMPNIFSTDFSGGALMDLGVYTLYAAIGILGTPRTARYVAQQLPSSIDLNGSGQLIYD
                     NYIVHIQAGKNITSNLASEIYTNDGTLTLNACQYISSAIFTKHDGEQIVLPIQAAQHP
                     MLEEALEIAQAIKQQNRKLVDQWLAVAESVHQTLYTMRQDAGIIFKADNNEI"
     gene            5010..6092
                     /locus_tag="U1099_001528"
     CDS             5010..6092
                     /locus_tag="U1099_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM4867841.1"
                     /translation="MKSKFPSSWTDQLTLLGFEDFTPIQVQAFEPIANGKSLLAISPT
                     GTGKTLAYLWPSLLALTPKKAQQLLILAPNTELAGQIFDVCKTWSETIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNINTIVLDEFDQLFSDSQYQFV
                     EKIINYVPRDHQLIYMSATAKFDRQKIAEDIESIDLSEQKLDNIQHCYMMVDKRERLE
                     TLRKFANIPDFRALAFFNSLSDLGASEDKLLYNGVNAVSLASDVNVKFRKVIIERFKN
                     HELNLLLATDMVARGIDIDNLECVLNFEVPFDQEAYTHRSGRTGRMGKEGLVITLVSS
                     PSELKQLKKYASVQEVILKNQELYKI"
     gene            complement(6159..7556)
                     /locus_tag="U1099_001529"
     CDS             complement(6159..7556)
                     /locus_tag="U1099_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM4867842.1"
                     /translation="MKKVSFWLGINIALLGIMVSLAVWLFAGLQERQVSQFIEEKQQT
                     ILAKGKGKIQEGNIDTTHVVAALPTDDAGHVLGPVESRMISYVQRRFGHKKPAGKIQN
                     LVFVSSIEGKTNFKNVTAREIQAEQYKVDNLQIKKQDKLPSERVLLTQDNKLFTLEDL
                     LPNLSSTASIIVGHLREALLAQGMKETDVEAIVKKFETLDLNAISFSYGDSQLTLQLP
                     DGYGINQLVLPISDLYPVVKSDYLVDADKVGYDEYMAAQVVDKKHLRQVALTFDDGPN
                     PNTTPVVLDLLKKYNAKATFFVVGKAVAGNEAILRRMVAEGHVIANHTWNHPNLVTIS
                     GEQVQREIQDTQAAITEATGIVPTMVRPPYGSVNQAVVNQIGLPSIYWSVDSKDWKSR
                     NPQAILKEIKEQTCPGSIILMHDIHQSTVDSLESVLQYLTGEGYNMVTVTDLLASPLN
                     PQLIYYSQELSGPAQ"
BASE COUNT         2210 a         1756 c         1439 g         2241 t
ORIGIN      
        1 taaaatagat tataccaaaa attcagtcaa ctggctgaat ttttatatgc tttcttgata
       61 ggtttcgatt cttttctaaa caagaaaaca ccaatccaat tggtgctttc ttaaggagat
      121 atgaaaaata tttaggattg aatatagtat aaaatatcta tcaaaactat tcatcagtct
      181 taggactgat attttctaat catttctaat ctcaactgta tctgcgagaa agccaaattc
      241 ttctgggact ggactggctt ctttttcgat ttcctgtgtc ttttgccctt ttgctttcgc
      301 cgaaaattca gcacgaatac ttacccaatc ttcctgagca atagctaaaa tctgtggcga
      361 aaaacctgcg gccttgctga gaatatttcc aaacatggta ttgagattgt cacgcttcat
      421 cgtctgttca gcattaagcg gagaatcaaa ggctagaatc gcatgatttt cattggcagc
      481 aactggttga gaaccaacaa gcaaggcacg atctgctcct gacagactcg cgataatttc
      541 tccccaagca ttctgcaagc gagtcaaatt ttcacgcgcc aaggttggat tttccatagc
      601 ttcctgcaaa attgcatgaa ccttgctggt atccaatcgg tacttcttcg gagcctgtgg
      661 cttgcgtgaa actggcttaa cagcacttgg ttgactagat aattgaccta gttgtttctg
      721 taaatccgca acttgttgtt gcagactggc tagttgcccc agtaaatcag ctggcagctc
      781 tgccatggcc cccgaaggag aactatcttc agccaaacga atggtcatca tttcagcgta
      841 aatcttcggt tgaggactgg acttgatgtc cgccaaaccc ctggtcacca tctctatcat
      901 ggtaaaaatc cgtgcctgct caagagctag attttctgaa aaacctgttg ttaaatgcgt
      961 atcctctcca cccgtctgca cgataaggat atctctcaaa tagtgcaaaa gatctgtcgc
     1021 aaaacggctc atactctttc cttggtcaaa taaggtttgt agatgctgga gagctgtgac
     1081 actatcccct tgacgcagac ttgccacata gtcatccaag gcacgcaagc tgatagaacc
     1141 cgtaatttcc tcagcaattt ccaaggtcac ttgctggtcc tgactaaggc tgagagcctg
     1201 atccaaaatg gatagggcat cccgcatccc cccttctgcc cggcgcgcaa tgattgtcag
     1261 agcctgctcg tcaaagatta gcccttcctt ggtcaaaatt gcagccaagt gctcctgaat
     1321 gtccgtcacc ttgatggact taaattcaaa tcgttgcacc cttgacaaaa tagtagcagg
     1381 aatcttgtgc aattccgtcg ttgctagaat aaagaccaca ttttcagtcg gctcttccaa
     1441 ggtcttcaag agggcattga aggctccagt agacagcatg tggacctcgt cgatgatata
     1501 gaccttatag gtcgcaagac taggtgcata ggtcgattta tcacgaatat cacgaatctc
     1561 atccacacca ttgttggaag ccgcatcaat ctcaatgaca tcttccaggc taccttccgt
     1621 aatagcctga cagatataac aatcgttaca aggctcgcct ccaacctgat tgggacagtt
     1681 catggccttg gcaaaaatct tagctgccga tgtcttaccc gtaccacgag gacctgaaaa
     1741 gagataggca tgactgattt ttccctgttc aatggcctgt ttgagcgtgg tcgcaaccac
     1801 ctcctgcccc accatctccc caaaggtctg gcttcggtac ttcctatata aagcttggta
     1861 cattagcgtt tttctccaaa catagcaaaa ttccagtccg tcttctcaac caagagagca
     1921 acgaactttt ctagatagtc ttggtcaatc gtatcataat cagctaccaa tctcgaatcc
     1981 aagtccaaaa cgcccagcaa ctggtcgttt ttcaccattg gcacaacgat ttctgatagg
     2041 gctgtcgcat cgcaggaaat atagttatca tgtagacgaa catcgtctac gagaatagtc
     2101 tggcgcttag ctgcagcctc accgcagaca ccctttccaa gtgcaatatg aacacaggaa
     2161 acgccaccct gaaaaggccc caaaatcaat tcactaccat catacaagta aaatcctgta
     2221 aagacagagt ttggtagagc ttgattgagc aaagcagaag catttgacag attggctaaa
     2281 gcattggttt cgccctctaa aagcgcctcc aattgggcca ataatagttg atagtttgaa
     2341 attttttctt gttttgtcat aggaacatta tagcatagtt aagccaactt ttatagtccg
     2401 agcgataaag aaaaaggctt acgcctattt ccacatccaa tacataatca ctgcatcaat
     2461 caatacccct atagcccagg ctagttgaaa atactttttc tgtgtcttat gccgaaaatg
     2521 ggttccacct gcccaggcac ccagcccgcc accaaaatag gacatgagta ataaggtctt
     2581 ttctgaaatc cgataggcgt tctttcttgc cttcccttta tccataccat aggtaatgaa
     2641 gacaactaaa ttccaaacta ctagcgccat actaaccatc tgtttgattg acatacattc
     2701 ctccaattaa gtcacatttc ttcttactct aacataatcc aaaacaggac acaaggaaaa
     2761 acgattcgga aatccgaatc gttacatttc cgccagtgta tcctctaacc atttgagctg
     2821 ggcacggttg cgttcaatag cccgtgtcaa aatcaaatag tgtccataat tgtcttggat
     2881 actttccttg gttgagaaca gctcaacttt acgactttct aggtgtttta agtgcttggt
     2941 gactagttca atttgactct gcaacaaacc aggaatccgt gggtcatcct tttcacggat
     3001 aaaaaacatc ttgattgaaa acaagtctct ttgctgaggc gtttcttcat ttggaatgga
     3061 tagccattcg tctaaaatct gacgaccagt agccgtcatc gcatactgct tttccttttc
     3121 atttccaggt actgcatgac aagtaatcaa ttcttccttg gtcatacgtt ttaattctgg
     3181 atagacttgg ctatggcaac ctgccaaaat tcgcctaaat cacgttgaac atagtcggta
     3241 atctgcttgc cagttaccat ttgaccgcta gcacccataa tccctaatat gatatgaggt
     3301 aatattcttt gcttcggcat cttaaccacg ctctcttaat atactctcca ccttggtgtt
     3361 aatcaagtcg atagctacaa cgttgctaac gccttcagga atgacgatat ctgcatagcg
     3421 cttggttggc tcgataaact ggtgatacat tggtttcacc accgaggtgt actgctcaat
     3481 gatactatct aagctacggc cacgctcttc catgtctctc ttaatgcggc gaatgatacg
     3541 aatatcgtca tcagtatcga cgaatatctt aatatccatt aaatcccgca accgtttgtc
     3601 ttccaatact aaaatacctt ccacaataaa tacatcttgt ggttcctgac gataggtctt
     3661 ctctgaacgt gtgtgctgag tgtaatcata aatcggaata tccactgaac gtcctgctaa
     3721 tagctcactg atatgataaa tcatcaaatc cgtatcaaaa gccagtggat ggtcataatt
     3781 ggttagtatc cgttcttcaa aggtcaggtg ggactgattc ttataataag aatcgtgttc
     3841 aatcatagat atacgagcat ttgggaaatt atccagaatg gcacgtgaca cacttgtctt
     3901 gccaccccca gatccgccgg ttacgccaat aataatgggt ttctgagtca tcatcatctc
     3961 cttctatttt caatactatt ttaccatgaa acatggtata atgaaaagga taacacaaaa
     4021 gaaatgagaa tttatgctta attttggtat tgttggcacc tcagacatct ctcatcgttt
     4081 tatagaggcg gcccatttaa gtggacatta tcagctacag gctgttttct cccgtaaact
     4141 agaaactgcc gctgcctttt cagagcagta tgaaggagtc gatttataca cagaatgggt
     4201 aaaatttctt tctgccccca ttgatgttgt ctatattgcc agtccaaatg ccctgcactt
     4261 tgaacaggca aaagctgttc tagctgctgg taaacatgct attgttgaga agcccatggt
     4321 ttctacacct caggaattag aacaattgcg tcgcgttgcc caagaaaata atgttttcct
     4381 ctttgaggct gctcgaaact atcacgaaca agctcaagcc attatccgtg aatttttgac
     4441 ggataaaacg attttaggtg gttcatttgg gtatgccaaa tattcttcta agatgcctga
     4501 attgttagct gggcaaatgc caaatatctt ttcaactgat ttttccggtg gtgctctcat
     4561 ggatttaggt gtctatacgc tctacgccgc tatcggcatt ttgggaactc ccagaacggc
     4621 ccgctatgta gcacaacagc taccttctag tattgactta aatggttcgg gacaactcat
     4681 ttatgacaac tatatcgtcc acattcaagc aggaaaaaat atcacgagca atctagcgag
     4741 cgaaatctat acaaatgatg gaacactaac actcaacgca tgccagtata tcagctctgc
     4801 tattttcaca aaacatgacg gagagcaaat tgttttacca atccaagcgg cccagcatcc
     4861 tatgctcgaa gaagcgctag aaattgcaca ggcaatcaag caacaaaacc gcaagcttgt
     4921 ggaccagtgg ttggctgtgg cagaaagcgt ccatcaaaca ctttacacca tgcgtcaaga
     4981 cgcaggaata atctttaagg cggataacaa tgaaatctaa atttccctct agctggacgg
     5041 accagcttac cctacttggc tttgaggact ttactcctat ccaagtccag gccttcgagc
     5101 ctattgcaaa cggcaagtct cttctagcta tcagcccaac aggcacagga aaaacccttg
     5161 cctacctctg gcctagccta ttagccctaa ctcctaaaaa agcccaacaa ttgctcatcc
     5221 tcgcacccaa tacagagctg gccggccaaa tatttgatgt ttgtaagact tggtcggaga
     5281 caattgggtt gactgctcaa ctattcttgt ctggttccag tcaaaaacgc cagattgagc
     5341 gactaaaaaa agggccagaa atcctgattg gaacacctgg tcgtattttc gagttgatta
     5401 aattgaaaaa aatcaagatg atgaatatca acaccatcgt cctagatgag tttgatcagc
     5461 tattctccga ttctcaatat cagtttgttg aaaagattat caactatgta cctcgtgacc
     5521 accagttaat ctacatgagt gcaacagcta aatttgaccg tcaaaaaatt gctgaagaca
     5581 ttgaaagcat tgatttatct gagcagaaat tggataatat ccagcattgc tatatgatgg
     5641 tagataagcg tgaacgtttg gaaactctac gtaagtttgc caatatccct gattttcgtg
     5701 ctcttgcatt ttttaacagc ctatcagacc ttggtgcaag cgaagataaa ctactctata
     5761 atggtgtaaa tgcagtttca ttggcttctg atgtcaacgt aaaattccgc aaggtcatca
     5821 tcgaacgttt caagaatcac gaactcaatc ttctacttgc tacagatatg gtcgcacgcg
     5881 gtatcgatat tgataactta gaatgtgtcc tcaactttga agttcctttt gaccaagaag
     5941 cctacactca tcgttctgga cgtactggac gcatgggcaa ggaaggtctt gtcatcaccc
     6001 tagtatctag tcctagcgaa ttaaaacaat tgaaaaagta tgcctccgtc caagaagtca
     6061 ttctcaagaa tcaagagctc tacaaaatat aatgaacaat atacaaaagg cttagaatca
     6121 tgttagtaga aacactggtt ctaagccttt tatcattctt actgggctgg gccagataat
     6181 tcttgtgaat aatagattaa ttgaggattg agaggactgg cgagtaagtc tgttacagta
     6241 accatattgt agccctcacc tgttaaatat tgcaatacag actcgaggct atcaactgtt
     6301 gactgatgaa tatcatgcat taggataata cttcctggac aagtctgttc ttttatttct
     6361 ttcaaaatgg cttggggatt gcgactcttc caatctttag aatcgaccga ccaataaatc
     6421 gatggaagtc ctatctgatt gacaacagcc tggttaaccg aaccataagg agggcgaacc
     6481 attgttggta caatacctgt agcttcagta atagctgcct gtgtatcttg aatttctctc
     6541 tgtacttgct ccccagaaat agtgactaga ttgggatgat tccacgtatg gttggcgatg
     6601 acgtgccctt ctgctaccat tctacggaga atagcttcat tcccagctac agccttacca
     6661 acaacaaaga aagttgcttt ggcattatat tttttcagta aatctaaaac taccggtgtt
     6721 gtgttcggat ttggtccatc atcaaaggtt agagcgacct gtctcaaatg tttcttatcc
     6781 acaacttgtg cagccatata ctcatcatag ccaaccttat cagcatctac tagataatca
     6841 ctcttcacta ctgggtacaa atctgaaatt ggtagaacca actggttgat gccgtaacca
     6901 tctggtaatt gtaaggttaa ttggctgtct ccataactaa aggaaatagc atttaagtct
     6961 agtgtttcaa attttttgac aatcgcttct acatcagtct ctttcattcc ttgagcaagt
     7021 agggcttctc ttagatgacc aacaataata cttgctgtag aggataaatt aggtaataaa
     7081 tcttccaggg taaacaattt attgtcttgc gtcaacaaca cacgctcaga cgggagttta
     7141 tcctgctttt taatttgcaa gttgtctact ttatactgct ccgcttgaat ttcacgagct
     7201 gttacatttt taaaatttgt ttttccctct attgatgaaa caaagaccag gttttgaatt
     7261 tttcctgctg gttttttatg accaaagcgt ctttgaacat aggaaatcat tcgactttct
     7321 acaggtccta agacatgacc tgcatcatct gtaggtaagg cagccacaac atgggtcgta
     7381 tcaatatttc cctcttgaat tttgcccttg cctttagcga ggatggtttg ttgtttttcc
     7441 tcgataaact gactaacctg tctttcttga agtcctgcaa ataaccatac agccaaactt
     7501 accattatgc cgagcaaggc gatgttgatt cccaaccaaa aactaacttt tttcatatcc
     7561 agcctcccat tcaaaacata tataaactat gctaaatcat ttaaaggctc ttgtcaagac
     7621 agttctataa aaactcataa aaagat
//
LOCUS       DASFSW010000093         7614 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.093, whole genome shotgun sequence.
ACCESSION   DASFSW010000093 DASFSW010000000
VERSION     DASFSW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.093"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            128..2104
                     /locus_tag="U1099_001530"
     CDS             128..2104
                     /locus_tag="U1099_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEM4867843.1"
                     /translation="MNFEEWIWALPSLSSFGEAIEKGLVMQTIFGKIVANQSAESVKT
                     YILNQQDAAYKELLLSCQNLLNDIFIFEDNWDMEPCSVGYHLAPLDWTADCHGDEEWT
                     FMLNRQEYLWKLVLAYLVEKDSRYMEQVKRFIFSWIEQVTNWHPKRTSSRTLDTAIRC
                     LSWLNILPFLIDWGGLSEAERSQLLVSIQKQLSYLHSHYRAKDTLSNWGIFQTGAMLL
                     AHAYIGDQVDLVELHHFAHQEIQEQIQTQILEDGTQFEQSFLYHVEVYKLLLGLAFSL
                     PDYRKQWTPILKKMADYVLQMTGPDGKSVALGDSDVHCTSDILQMTAIFFEDGTYLVD
                     NSKLDLGVLFLVGENGLRIFEHLTAQPNSSGGQHFSESGHSIVKEVDSYLCFKAGPVG
                     SAHSHSDQNSLCFYHKGQPIVIDSGRFSYRECEERYLLKSAWAHSTCILDDNAPEQIK
                     GSWEYDTYPQFISHSYKGVERCHWMQGIYQAQTSQGLSYWHKRQLLMVGGDLLLIVDS
                     VDCSGQHELISQLILDERVKIGEERLNDLRLYSQKDFEIQKTVRSPRYNQLVETNKLV
                     KKESFQDRLVTFTLLADEAIRVKQLPVYQTDYHLIESGLAFSCQGKGLNILLVVSDND
                     IYKGEKLLQVAGYKLRGKCLVVDRDKRQVVRLKN"
     gene            2200..3201
                     /locus_tag="U1099_001531"
     CDS             2200..3201
                     /locus_tag="U1099_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM4867844.1"
                     /translation="MSEKFTIKDIAELAQTSKTTVSFYLNGKYEKMSKETRERIERVI
                     RETDYKPSVVARSLNSKNTKLIGVLIGDITNTFSNQIVKGIEEEAHKSGYQVIIGNSN
                     YNQESEDKYIESMLMLGVDGFIIQPTSHFRKYSRIIEDKKKHMVFFDSQLYEHRTSWI
                     KTNNYDAVYDATQLCVENGYQKFILITADISRLSTRIERATGFMDALLDCKQSYQDLT
                     IADENTDIEEIKRFLQGVVNPQEKTLVFVPNCWALPLVFNAMKELKYDFPQVGLLGFD
                     NTEWTNFSSPTISTIVQPAFEEGCQATRMLINQIEGGNQVGRQQVLDCTVHWKDSTF"
     gene            complement(3637..4596)
                     /gene="nrdF"
                     /locus_tag="U1099_001532"
     CDS             complement(3637..4596)
                     /gene="nrdF"
                     /locus_tag="U1099_001532"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEM4867845.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLTAEEKDLVGKVFGGLTLLDTLQSETGVQALRNDIRTPHEEAVYNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEDIFEWTNSNEYLQRKAKIINEIYETGTPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELSEEEQDKLR
                     DWMYDLLYQLYENEEGYTRSLYDAVGWTEEVLTFLRYNANKALMNLGQDPLFPDSADD
                     VNPIIMNGISTGTSNHDFFSQVGNGYLLGEVEAMTDDDYLYGL"
     gene            complement(4726..6885)
                     /gene="nrdE"
                     /locus_tag="U1099_001533"
     CDS             complement(4726..6885)
                     /gene="nrdE"
                     /locus_tag="U1099_001533"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEM4867846.1"
                     /translation="MSLKELGDVSYFRLNNEINRPVNGQIPLHKDQEAVKAFFKENVL
                     PNTKQFDSILDKIAYLLEENYLEKEFLDQYSPEFIIKIDQFLKDQNFRFKSFMAAYKF
                     YNQYALKTNDGAYYLESMEDRVLFNALYFAEGNEELALNLANEMIHLRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYSVELEYGVPYSYLDITEKYDELVANPRI
                     RKTKIKARDLETEISKLQQESGYPYVINIDTANRSNPVDGKIIMSNLCSEILQVQTPS
                     VINDSQEYLTMGTDVSCNLGSTNIVNLMKSPDFGRSVRTMTRALTYVTDHSHISAVPS
                     IEAGNSQAHSIGLGAMGLHSYLAQNLIDYGSKTAVEFTNIYFMLLNYWTLVESNNIAR
                     ERKATFHNFEKSKYADGTYFDKYVTGDYQPQSERVKELFEGIFIPSGQDWADLREKVK
                     ADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLATETI
                     PFYKSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPKELYEWKKENKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(6918..7136)
                     /gene="nrdH"
                     /locus_tag="U1099_001534"
     CDS             complement(6918..7136)
                     /gene="nrdH"
                     /locus_tag="U1099_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEM4867847.1"
                     /translation="MVTIYSKNDCVQCKMTKKFLDQHSVDYKEINLDEQPEFIDHVKV
                     LGFSAAPVIETENDVFSGFQPGKLKALV"
BASE COUNT         2280 a         1381 c         1676 g         2277 t
ORIGIN      
        1 agttgattaa atcagattcg gagtatgaaa tacgaattgc tgaaatttgt tttggaactc
       61 ctatctccaa cctttaacag tccactgaac tgttaaagcc aatcaatcac tgcgctaaga
      121 tgttgatatg aactttgagg agtggatctg ggctttgccc agcctctctt cttttggaga
      181 agcgatagag aaaggacttg ttatgcaaac aatattcggc aaaatagtag ccaatcagtc
      241 agcagagtcg gtcaaaacct atattctcaa tcaacaagat gcagcttata aagagcttct
      301 cctttcttgt caaaatttat taaatgatat ttttatcttt gaagacaatt gggatatgga
      361 accttgttca gtcgggtatc atcttgctcc tcttgactgg acagccgact gccatgggga
      421 tgaggaatgg acctttatgc tcaatcggca ggaatacttg tggaaattag ttttggccta
      481 tctggttgaa aaagattcgc gctacatgga gcaggttaaa cggtttatct ttagttggat
      541 agaacaagtg acaaattggc atcctaaaag aacgagcagt cgcaccttag atacggctat
      601 ccgctgtcta agctggctca atattctccc atttttaatt gattggggcg gactaagtga
      661 agctgagcgt agccaactgt tagtatccat tcaaaaacaa ttatcttatc ttcacagtca
      721 ttaccgtgcc aaagatacgt tgagcaattg gggaattttc cagactggcg caatgctctt
      781 ggcccatgct tatatcggtg atcaggtgga tttagtagag cttcaccatt ttgctcatca
      841 ggaaatccaa gagcaaattc aaactcagat attggaagat ggaactcagt ttgagcagtc
      901 ttttctctac catgtggaag tttataaact cttgctagga ctagctttca gcttgccaga
      961 ttatcgaaaa cagtggacac ctattttgaa aaagatggca gactatgtct tacagatgac
     1021 gggaccagat ggcaaaagtg tggctcttgg cgatagcgat gttcattgta cttctgacat
     1081 cttacagatg actgcgattt tctttgaaga tggcacctac ctagtagata attccaagct
     1141 agatttaggc gttctcttct tggtcggaga aaatggcttg cgcatatttg aacacttgac
     1201 cgctcaaccc aactcgtcag ggggacagca tttttctgaa tcaggtcaca gtattgtcaa
     1261 agaagtggac agctatttat gtttcaaggc cggtcccgtg ggaagtgccc acagccactc
     1321 tgaccaaaat agcttgtgct tttaccataa aggacaacca atcgtaattg acagtggtcg
     1381 tttcagctac cgtgaatgcg aggagcgcta cttgcttaaa agtgcttggg ctcacagcac
     1441 ttgtatcctt gatgacaatg ctccagagca gataaaaggt tcatgggaat atgacacgta
     1501 tcctcagttt attagtcaca gttataaggg ggttgaacgc tgtcattgga tgcagggcat
     1561 ttatcaggct cagaccagcc aaggcttgtc ttactggcat aaacgacagc tccttatggt
     1621 tggaggcgat ttattgctga tagtggatag cgttgattgc tcaggccagc atgagttgat
     1681 cagtcaattg attttagatg aaagagtcaa gattggagaa gagcggttaa atgatcttcg
     1741 gctatatagt caaaaggatt ttgaaatcca gaaaaccgtg cgttctcctc gatacaacca
     1801 actagtagag accaataaac tggtcaaaaa agagagtttt caagatagat tagttacatt
     1861 tactctatta gcagatgaag caattcgtgt caagcaattg ccagtttacc aaacagacta
     1921 tcatttgata gaaagtggtt tggcttttag ctgtcaagga aagggactga atattctgtt
     1981 agtggtttct gataatgata tatataaggg agagaaattg cttcaagtgg ctggttataa
     2041 gttgagaggt aagtgcttag tagtggatag ggataagaga caagttgtcc gactcaaaaa
     2101 ttagaaggga cagaaaaaat acagtcattt ctgtgcaaaa taactgataa agtacggata
     2161 gtatgataga ataaagtaac taaaggaggt atcggtaagg tgtcagagaa gtttaccatc
     2221 aaagatattg cagaactggc tcagacttca aaaacaacag tttcctttta cttaaatggt
     2281 aaatatgaaa agatgtccaa agagacacgc gaaagaattg agagagtcat tcgcgaaaca
     2341 gactataaac ccagcgttgt tgcgcggagc ctaaattcca aaaatactaa gttgattgga
     2401 gtattgattg gtgatattac aaatacattt tccaatcaaa ttgtcaaagg tatagaagaa
     2461 gaagcgcata agagtggcta tcaagttatc atcggaaaca gcaattacaa tcaagaaagc
     2521 gaagataagt atattgagag catgctgatg ttgggtgtag atggttttat tattcagccg
     2581 acatcgcatt ttagaaaata ttcgcgcatt attgaggata agaaaaagca tatggttttc
     2641 tttgatagtc agctttacga acaccgtacc agttggatta agacaaataa ttatgatgct
     2701 gtctatgatg caactcaact atgtgtagaa aatggttatc aaaaatttat tcttattaca
     2761 gctgacatta gtcgcttaag tacacggatt gaacgagcta cagggtttat ggatgctctg
     2821 ttagattgta aacaaagtta tcaagattta acaattgcag atgaaaatac ggacatagag
     2881 gaaattaaac gttttcttca aggggtggta aatcctcagg aaaagaccct agtattcgtt
     2941 ccgaattgct gggcgttacc cttagttttt aatgctatga aagaacttaa atatgacttt
     3001 cctcaggttg gtcttttggg gtttgacaat actgaatgga caaatttttc atcccctact
     3061 atctctacca ttgttcaacc cgcctttgaa gaagggtgtc aggcaacaag gatgctgatc
     3121 aatcagattg agggcggaaa ccaagtggga cgacaacagg ttttagattg cacagttcat
     3181 tggaaagatt ctacattcta agttaagtca aattttactt cttaggaaga ggagcagaac
     3241 gaagtagaat attctgattg agagctcact gaaaccctcc ttgaatcgcc taatatatac
     3301 aaaaaatagg aattttccct taactggtgg gagaattcct atttttatgc caatctcatc
     3361 gagatgatat attaacatta tatacgattt taaactcaac agatttctat agtcttttag
     3421 tactaggcaa tgagccgcag acagtactgg agtgccgcac agttgattag gttggattta
     3481 gagtgtaaaa cacgaacaaa tccgccaatc aaccactgcg ctgagatgtt gacacgaact
     3541 ctaagaagtg gcgctgggct ttttgcccag cctcacgtca aagataaaca aaatgactat
     3601 aaaacggaaa aatagaaatg atatttttcc taagatctac aacccatata aataatcatc
     3661 atctgtcata gcttccactt caccaagaag atagccgtta ccgacttggg agaagaagtc
     3721 gtggttagat gtaccggttg agataccgtt catgatgatt gggttgacat catctgctga
     3781 atctgggaag agtggatcct gacccaagtt catgagggcc ttgttggcat tgtaacgaag
     3841 gaaagtcaag acttcctcag tccagccgac tgcgtcatag agggagcgtg tgtagccttc
     3901 ttcattttcg taaagttggt agagcaggtc gtacatccag tcgcggagtt tatcttgttc
     3961 ttcctcggac aattcattga aaccaagttg gaacttgtaa ccgatgtagg taccgtgcac
     4021 agactcgtca cggatgatca gcttgatgat ttctgcaacg ttggccagct tgttgttacc
     4081 aaggtagtag agtggcgtga agaaaccaga gtagaagagg aaggtttcta ggaatacgct
     4141 agctactttc ttttcaagag gtgtacctgt ttcgtagatt tcgttgataa tctttgcctt
     4201 gcgttgcaaa tattcgttgc tgttggtcca ttcaaagatg tcttcaatct cagacttggt
     4261 gttcaaggtt gagaagatag aagagtaaga cttggcatgg actgactcca tgaattggat
     4321 gttgttgtaa acagcttctt cgtgcggtgt acgaatatcg ttgcggaggg cttggacacc
     4381 tgtttcagat tgaagggtat ccaagagggt caatccacca aagaccttac caaccaggtc
     4441 tttttcttca gcagtaagtt tacgccagtc atccaagtcg tttgataaag gaatacgtgt
     4501 atcgagccag aattgctcgg tcaacttttc ccaagtagac ttgtcgataa catcctcaat
     4561 ggcgttccag tttatggctt tgtagtaggt ttccatcata ttctctttct ttttcttata
     4621 cttcaaatat tatatcacaa atagatagta gcctgtaaaa aaatccccta aaagggattt
     4681 taatcgattg cttattagtt gcttcacatc gtgaacgata gtgtcttaaa tcacacaact
     4741 ttcacattgg tttgcgccga cttcttcacc gtcatcggta aaggtacgga tgtagtagat
     4801 agacttgata cctttgttga aggcgtagtt acgaaggata gagaggtcac gtgtagtttg
     4861 tttgttctct tttttccatt catagagttc ttttgggagc tcgctgcgaa ggaaaagagt
     4921 gagtgacaac ccttggtcga cgtgttctgt tgcggcagca tagacatcga taaccttccg
     4981 catatccatg tcataggcag atttgtagaa tggaatggtt tctgttgcca agccagctgc
     5041 tggatagtag attttaccga ttttcttctc ttggcgttct tcaatccgtt gtgtgattgg
     5101 gtgaattgaa gcagaaacgt cgttgatata gctgatagat ccatttggag caacagctaa
     5161 gcggttttga tgatagagac cgtctgcttt tactttttcg cggaggtcag cccaatcttg
     5221 tccacttgga atgaaaatgc cttcaaaaag ttctttaaca cgctcggatt gtggttggta
     5281 gtcacctgtc acgtatttat cgaaataagt gccgtctgca tattttgatt tttcaaagtt
     5341 atggaaagta gctttgcgtt cgcgagcaat gttatttgat tctaccaagg tccaatagtt
     5401 aaggagcatg aagtagatgt tggtaaattc tacggcagtt tttgaaccgt aatcaatcaa
     5461 gttctgagct aagtaagaat gaagtcccat agctccaaga ccgatagaat gtgcctgact
     5521 gtttcctgct tcaatagatg gtacagctga aatgtgtgaa tgatctgtga cataagtcaa
     5581 agcacgagtc atggtacgga cagaacgtcc aaagtcaggc gatttcatca agttgacaat
     5641 gttggttgaa ccaaggttac atgatacatc tgtccccatt gtcaagtatt cctgagagtc
     5701 attgatgacg cttggtgttt gaacttgaag gatttccgaa cagaggtttg acatgataat
     5761 cttgccgtca acagggttgc tacggttagc tgtatcgatg ttgataacgt atggatagcc
     5821 agactcttgt tggagtttgg aaatttctgt ttccaagtca cgtgccttga tttttgtctt
     5881 acgaatgcga gggttcgcca ccaattcatc gtatttttct gtgatgtcaa ggtagctata
     5941 agggacaccg tattccaact caactgagta tgggctgaag aggtacatat cttcgttttt
     6001 acgtgccaat tcgtagaatt tatcaggaac ggtgataccg agtgataagg tcttcacgcg
     6061 aactttctca tcggcatttt ctttcttagt cgaaaggaag gaaataatat ctgggtggaa
     6121 aacatcaaga taaacgacac cagccccttg gcgttgccca agctggttag aataggagaa
     6181 gctgtcttcg aagagtttca taacaggaac aaccccagaa gctgccccct catagccctt
     6241 gattggagca cctgcctcac gcaagttgct aagggaaata ccaacaccac caccgatacg
     6301 ggacaattgc agagcagagt tgatagaacg tccaatcgag ttcatatcgt cagtgacctg
     6361 gatgaggaaa caagacacca actcaccacg acgggcacgg cctgcgttga ggaaagacgg
     6421 tgttgcaggc tggtaacgaa gatgaatcat ttcgttggcc aggtttagtg ctaattcttc
     6481 gttgccttca gcaaaataca aggcgttgaa gagaacacgg tcttccatac tttccaagta
     6541 gtaagccccg tcgttggttt tgagggcata ctgattatag aacttgtagg ctgccatgaa
     6601 agacttgaag cggaagtttt ggtctttcaa gaattgatca attttgatga taaattctgg
     6661 gctatactgg tccaaaaatt ctttttcaag atagttttct tctaatagat aggcaatctt
     6721 gtctaaaata ctatcaaatt gcttggtatt tgggaggaca ttttctttaa agaaggcctt
     6781 aaccgcttct tggtccttgt ggagtgggat ttgaccatta acaggacggt tgatttcgtt
     6841 atttaaacgg aagtaggaca catcgcctag ctcttttaaa ctcatagtcg caatctcttt
     6901 ctataaacag tagggagtta aaccaaggct tttagttttc ctggttgaaa acctgagaaa
     6961 acatcatttt cagtttcgat aacaggtgca gctgagaaac cgagaacttt tacgtgatcg
     7021 ataaattctg gctgttcatc tagattgatt tctttataat ctacactatg ttggtccaag
     7081 aatttcttgg tcattttaca ctgtacacaa tcattttttg aatagatcgt taccattcaa
     7141 tttctccttt gtccatgagg gattaataaa aaatcccaat tttgttacca atataggata
     7201 caagatttta aggttaaagt caagaaggaa gtttgtttaa aatcacaaaa tatagtgcac
     7261 aatattttta taagacacca tatattgtac atctgtccaa tgctgccaag tcgaaaaata
     7321 gcgtttacat agctgtctct atttagtttg ataatcctag caatctctcg gtaaagtata
     7381 aattagcttt tttacaaatg tcataatatt ttgaaggaaa catacaatgt tataaaatat
     7441 aacatttata gttatacatg tattgaatat atattagctt ttgtactata ctattcaata
     7501 atgtcgaaag aggttataaa atatggaaaa atcacataga aaacgcttga ttcataaagg
     7561 ggaagaatat tcatattatg cattggattc gatatcaatg gaagaaaaag tgga
//
LOCUS       DASFSW010000094         7520 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.094, whole genome shotgun sequence.
ACCESSION   DASFSW010000094 DASFSW010000000
VERSION     DASFSW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.094"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..430)
                     /locus_tag="U1099_001535"
     CDS             complement(<1..430)
                     /locus_tag="U1099_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HEM4867848.1"
                     /translation="MNDLNTTILQKASGETRLNPDEQRLYMGTYRERVVLIVSFDDLS
                     SEVVGNKFDTICQKLANRYSPLFLKLSPALSDKQQISLLKIAQNFGITTAIIDEKIGQ
                     SPYALVFHTNHAVDNEEVSLESIFPNLISKPEEKKEDAKPS"
     gene            580..1887
                     /gene="eno"
                     /locus_tag="U1099_001536"
     CDS             580..1887
                     /gene="eno"
                     /locus_tag="U1099_001536"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEM4867849.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNVIADAIIGFDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPSFKEGLRWGAEVFHALKKILKARGLVTAVGDEGGFAPKFEGTEDG
                     VETIIEAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGKRVQLVGDDFFVTNTDYLARGIK
                     EGAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAVYKGLNSFYNLKK"
     gene            complement(2047..3210)
                     /locus_tag="U1099_001537"
     CDS             complement(2047..3210)
                     /locus_tag="U1099_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4867850.1"
                     /translation="MRIKDKIKKNGQKVYYASVYLGVDQLTGKKARTTVTATTQKGVR
                     VKARDAINAFAANGYTVKDKPTITTYKELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPIIQQQVNKWADKANTGQKGAFTNYSLLHNMNKRILKYGVSMQVI
                     TYNPANDVIVPRKQQKEKAGVKYLDNKELKQFLDYLDTLDQSNYENLFDVVLYKTLLA
                     TGCRIGEALALEWSDIDLDNGIVSINKTLNRFQEINSPKSSAGYRDIPIDNATLLMLK
                     QYKNRQQVQSWQLGRSETVVFSVFTEKYAYACNLRQRLNKHFKAAGVTNVSFHGFRHT
                     HTTMMLYAQVSPKDVQYRLGHSNLMMTENVYWHTNQENAKKAVSNYETAINSL"
     gene            complement(3294..3839)
                     /locus_tag="U1099_001538"
     CDS             complement(3294..3839)
                     /locus_tag="U1099_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867851.1"
                     /translation="MNRLKDLRTKHKLTQQQTADIAGVTRRGYQKWESGESSIKADKA
                     QILADYFGVPVAYLLGYHEETISFTFPNDDEGFYKETQKLIDIADNIDPNSEIYSQVQ
                     QVKDMAILARSSREKIKARYGQKVYAGLNDKETDFEFYQYLGFLLNKEEYLKSDLLVY
                     LSLLDSSDKEIVFKMITEFKK"
     gene            3997..4185
                     /locus_tag="U1099_001539"
     CDS             3997..4185
                     /locus_tag="U1099_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEM4867852.1"
                     /translation="MIITETQAKAIRRKIADKQLKQYEFAKEIGVASRTVPKIVAGNY
                     KAPKRIYAAVMEWLAKDY"
     gene            4364..4639
                     /locus_tag="U1099_001540"
     CDS             4364..4639
                     /locus_tag="U1099_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867853.1"
                     /translation="MALSTRNIKQQGNQIAELLSRIEIIQQLGNALLLADNAGADSAT
                     LHYQMKQAFSVIFEMTEQLYKDLDLIACKLINCDDDKELEVIRQHER"
     gene            4629..4832
                     /locus_tag="U1099_001541"
     CDS             4629..4832
                     /locus_tag="U1099_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867854.1"
                     /translation="MNDKILSNYELLCTELENVISTLEIASGDIEPGKATALVTVATN
                     GLKHLVSEHTELSDKFFKEYGNE"
     gene            4825..5028
                     /locus_tag="U1099_001542"
     CDS             4825..5028
                     /locus_tag="U1099_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867855.1"
                     /translation="MNELIPTLTESVILIIVSLAILILLWRYESYIELDISPQTDKVE
                     ENTTAHVQERYGAYVWLAGKKFN"
     gene            5039..5305
                     /locus_tag="U1099_001543"
     CDS             5039..5305
                     /locus_tag="U1099_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867856.1"
                     /translation="MSTFSVEFEQGLLDRVDKLAQQKLELEKKLQKKTGLITAKELKD
                     DLDISGTTLKNWMDIGLVSYQSPFESSKKLYFKVSDVINFLTVR"
     gene            5316..5474
                     /locus_tag="U1099_001544"
     CDS             5316..5474
                     /locus_tag="U1099_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867857.1"
                     /translation="MKVFIIDGDAWRGKAHYSPSLDVVFISDKVDKHARDELIERVTK
                     SSNRTIWR"
     gene            5479..5694
                     /locus_tag="U1099_001545"
     CDS             5479..5694
                     /locus_tag="U1099_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867858.1"
                     /translation="MAKEHYEVTSVFPAGWIVNGKKLTEPIEMSAEELRKSEVYPPEN
                     SSVYDIFKKINEERWEETRKDGNKAND"
     gene            5708..6502
                     /locus_tag="U1099_001546"
     CDS             5708..6502
                     /locus_tag="U1099_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM4867859.1"
                     /translation="MTDNFLDLPPTTKVLYFFLNLEADDDGFVGNPKTVMRLTETTKD
                     DMKLLIDSEYVLLFDTGVVVITDWTEHNAIRKDRKKDTRFTKEMQQIILVDGGKYFWL
                     SDSQPNDNQSATINTPNGCVGEDRRGKDSIGKERVVEVDKEQNQSPTPPPFNQDFVNL
                     YKSFEAETGKALSPIQMQELQYMLEDFSADVIHEALREAVGQGKANFAYIQAILKRWK
                     QDNLLTVELIRNNKAAREVKKQQNNSKPEPQTREEWLANWSEENPF"
     gene            6518..7363
                     /locus_tag="U1099_001547"
     CDS             6518..7363
                     /locus_tag="U1099_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM4867860.1"
                     /translation="MLTQAEFIANTKTLEETCPIHGIPLMQLDRVVKIAEEDKPRKLS
                     PICPECIKEQNEKQVQEEVEKHLNAGIYQKTYNVLMRDSTIPEDLKAASFDNFIIETP
                     EEKQMLDFVKNQTQKYLDGMNGNTLLTGTTGVGKTHLAISLAKELNEAYRAKGEPKSV
                     LFINLTEILREIRESFKFTSKEGYYSRMLKEVDYLILDDLGVKLGNASGQSKSAWEEE
                     FIFDVLSHRNNTIITTNLSNDEIANLYSERVASRVRTGLDGNYFKVFSIKDKRYSINK
                     LKSNM"
BASE COUNT         2393 a         1331 c         1555 g         2241 t
ORIGIN      
        1 aggaaggttt tgcatcttcc tttttttctt ctggttttga gataagattt ggaaatatgg
       61 attctaagct aacttcttca ttatccacag cgtgatttgt gtgaaagaca agtgcgtagg
      121 gagactgtcc tattttttca tcgatgatag cggtagtaat accgaagttc tgagcaattt
      181 ttagtagtga aatttgttgc ttgtcagaca gggctggcga gagtttgagg aatagaggag
      241 agtatctgtt tgctagtttt tgacaaatcg tatcgaactt atttccgacg acttcactac
      301 tcaaatcgtc aaaggaaacg atcaggacaa cccgttcgcg ataggttccc atgtatagac
      361 gttgttcatc tggattcaat cgtgtttcgc cagaggcttt ctgtagtatg gtagtattta
      421 aatcgttcat ggtttcagta taacatactc agttaacttt gaaaaggaag gcgtttacat
      481 tatttctttc agtaaaataa cctgtttttt cttgaaaaat cccttctttt agggtatgat
      541 agctatgtaa caattttaaa ctcaaaggag agtaatataa tgtcaattat tactgatgtt
      601 tacgctcgcg aagtccttga ctcacgcggt aaccctacac ttgaagttga agtttatact
      661 gaatcaggtg ctttcggacg tggtatggtt ccttcaggag cttctactgg tgagcacgaa
      721 gcagttgagc ttcgcgacgg tgacaaatct cgttaccttg gtcttggtac tcaaaaagct
      781 gttgacaacg tgaacaacgt gattgctgac gctatcatcg gttttgacgt tcgtgatcaa
      841 caagctatcg accgcgctat gatcgctctt gacggtactc ctaacaaagg taaattgggt
      901 gcaaacgcaa tcctcggtgt ttctatcgct gttgcgcgtg ctgctgctga ctaccttgaa
      961 gtgccacttt acacttacct tggtggattc aacactaaag tattgccaac tccaatgatg
     1021 aacatcatca acggtggttc tcactcagac gctccaatcg ctttccaaga attcatgatc
     1081 ttgccagttg gcgctccttc attcaaagaa ggtcttcgtt ggggtgctga agtattccac
     1141 gctttgaaga aaatcttgaa agctcgtggt ttggtaacag ctgttggtga cgaaggtggt
     1201 ttcgctccta agttcgaagg aactgaagac ggtgttgaaa caatcatcga agctatcgaa
     1261 gctgctggtt acgaagctgg cgaaaacggc atcatgatcg gtttcgactg tgcgtcatct
     1321 gaattctacg ataaagagcg taaagtttac gactacacta aattcgaagg tgaaggcgct
     1381 gctgttcgta cttctgcaga acaaatcgac taccttgaag aattggttaa caaataccca
     1441 atcatcacta tcgaagatgg tatggatgaa aacgactggg atggctggaa agctcttact
     1501 gagcgtcttg gcaaacgcgt tcaattggtt ggtgacgact tcttcgtaac aaacactgac
     1561 taccttgcac gtggtatcaa agaaggtgct gctaactcaa tccttatcaa agttaaccaa
     1621 atcggtactc ttactgaaac atttgaagct atcgaaatgg ctaaagaagc tggctacact
     1681 gccgttgtat cacaccgttc aggtgaaact gaagattcaa caatcgctga catcgcagtt
     1741 gcaactaacg caggccaaat caagactggt tcattgtcac gtacagaccg tatcgctaaa
     1801 tacaaccaat tgcttcgtat cgaagatcaa cttggtgaag ttgcagtcta caaaggcttg
     1861 aactcattct acaacttgaa aaaataatct ctttatgggg ttaagggctg taaagccttg
     1921 atattaggca ctttggggca tcatcccaag gtgctttttt agaatttact accctttgta
     1981 ctaccccatg attgaaatgc tgtatttttt gtatagtaag agggcagtca caaagtgtga
     2041 ccccctttat aaactattga ttgctgtttc ataatttgag acagcttttt ttgcgttttc
     2101 ttggttagtg tgccaataaa cattttcagt catcatcaag ttagagtgac ctagcctgta
     2161 ttgtacatct ttggggctaa cttgagcgta gagcatcata gtagtatgtg tgtgacggaa
     2221 accatgaaat gatacattag tcacgccagc agccttaaaa tgcttattta ggcgttgtct
     2281 caaattacaa gcataggcat acttttctgt aaatacagag aatacaaccg tttcagaccg
     2341 ccctagttgc catgattgta cttgctggcg gttcttgtat tgtttgagca taagtaatgt
     2401 ggcattgtct attggtatat cacgataacc agcgcttgat ttaggagagt ttatctcttg
     2461 aaatcggttg agtgtcttgt tgatgctgac aataccgtta tctaggtcaa tatcagacca
     2521 ttcaagagct agtgcctccc cgatacggca accagtagct aacaaagtct tgtataggac
     2581 aacatcaaac aagttttcat agtttgattg gtctaaggta tcgagataat caagaaattg
     2641 ttttagttct ttgttgtcca ggtattttac accagctttt tctttttgtt gcttacgtgg
     2701 cactataaca tcattagctg ggttatacgt tatcacttgc atagaaacac cgtatttcaa
     2761 aatacgctta ttcatgttat ggagtaagga gtagttagta aacgcccctt tttgccctgt
     2821 attagccttg tcagcccatt tgtttacttg ttgttggata ataggcgtag tgagtttaga
     2881 tagcttgtaa tcgccaaata caggcaatag atgcactcta accaacccat ccattgattg
     2941 gcgagtattt ggcttaactg tattcttgta actatcccac caaactttta caagctcctt
     3001 gtacgttgta attgtcggct tgtctttaac tgtatagcca ttagcagcaa aagcattgat
     3061 agcatcacgc gcttttaccc taaccccctt ttgggtggtt gctgtaacag ttgtacgggc
     3121 tttcttgccc gttagttggt caacgcctag ataaacacta gcatagtaaa ctttttgacc
     3181 gttctttttg attttatctt tgattctcat gtatttgtac ctttctttcc atcagcaggc
     3241 aaggcgcgtg gttttgttag gtatttatac acggcttcat agcaagacaa ttactatttt
     3301 ttaaattcgg taatcatttt aaaaacaatc tctttatccg atgaatcgag taatgaaaga
     3361 tacaccagca aatcactttt gagatattct tctttattta atagaaagcc tagatactgg
     3421 taaaattcaa aatctgtttc tttatcattt agcccagcat atactttttg accatatcga
     3481 gcttttattt tttcacgact actccgtgct agtatagcca tgtctttaac ttgctgcacc
     3541 tgggaataaa tttcactatt tgggtcaata ttgtcagcta tatcaattag cttttgagtc
     3601 tctttataga aaccctcgtc atcatttgga aaagtaaaac tgatagtttc ttcatgatag
     3661 ccgagtagat aagcaacggg gacgccgaaa taatcagcta atatttgtgc tttgtcagct
     3721 tttattgagc tttcaccact ttcccatttt tggtaacctc gtctagttac accagcaata
     3781 tcagccgttt gttgttgggt aagtttgtgc ttagtacgta aatcttttaa tctattcact
     3841 ttttttcacc tcatagcgat tataacacaa tttcacgaga taatgctaac tttttttcac
     3901 ttttttatca taaacgcttg acaagggaac gaaagttagc taaaatgcta tttgtaatat
     3961 atttgctaac taaagttagc tagaaaagag gaaattatga ttattacaga gacacaagcc
     4021 aaggctattc gtcgaaagat tgctgataag cagctaaagc aatatgaatt tgctaaagaa
     4081 ataggtgtgg ctagtcgtac agttcctaaa atagtagctg gtaactataa agcaccaaag
     4141 cgaatttatg cagcagtcat ggaatggtta gcaaaagatt attaaggtac aaaaaagcca
     4201 tgtacaggcg accaaaccaa cgtacacggc taaggaaaaa taacaaaact caagcaaagg
     4261 catggcgcgt ggttttgtta ggtatttagt aagagtgaaa actatcgccc ttataaaata
     4321 tatttactat aagtttacca aaaacaaagg agaaaatcaa aaaatggcat taagcacacg
     4381 aaacattaag caacagggta accagattgc tgagttgctc tcacgtattg aaattatcca
     4441 acaactaggc aatgctttat tacttgctga taatgctgga gcagatagcg ctactttaca
     4501 ctatcaaatg aaacaagcat ttagcgttat ttttgaaatg actgaacagc tatataaaga
     4561 cttagattta atcgcttgta agttgataaa ttgtgatgat gataaagaat tggaggttat
     4621 tagacagcat gaacgataag attttaagta actatgaatt gctatgtact gagttagaaa
     4681 atgttatcag tactttagag atagctagcg gcgatattga accaggcaag gcaacagccc
     4741 ttgtaacagt agcaaccaat gggctaaagc atcttgtttc agaacacaca gaattatcag
     4801 ataaattttt caaggagtat ggcaatgaat gaattaatcc caacattgac tgaaagtgtt
     4861 atcttgatta ttgtttcctt ggctattcta attttattat ggcgttatga gagctatata
     4921 gagcttgata ttagcccaca aactgacaag gtggaggaaa acaccacagc ccacgtacaa
     4981 gagcgctatg gggcttatgt atggcttgct ggtaagaaat ttaactagga gggggaatat
     5041 gtcgacattt tcggttgaat ttgaacaagg cttattagat agagttgata agctagcaca
     5101 gcaaaagcta gagctagaga agaagttaca aaagaaaaca ggtttaatca cagccaagga
     5161 actcaaagat gacctcgata tttctggcac aactttgaaa aactggatgg atattgggct
     5221 agtctcatat caatcaccct ttgaaagtag caaaaaacta tatttcaagg tttctgatgt
     5281 gattaatttt ctcacagtac gctaggaggt atattttgaa agtatttatt attgatggtg
     5341 atgcgtggag aggtaaggcg cattattcac ctagcttaga tgttgtgttt atcagtgaca
     5401 aagtggataa gcacgcgcgt gatgaattga ttgagcgtgt gacgaaatca agtaatagaa
     5461 caatctggag gtaaaattgt ggctaaagaa cattacgaag taacaagtgt ctttccagca
     5521 ggctggattg ttaacggaaa gaaactaaca gaacctattg aaatgtcagc agaagaacta
     5581 agaaaatccg aggtatatcc gcctgaaaac agttcggtat atgacatttt caaaaaaata
     5641 aacgaagaga gatgggagga gacgcggaaa gatggcaaca aggcgaatga ttagcaagga
     5701 agttgttatg actgacaact ttcttgattt acctcccact acaaaagttt tgtatttttt
     5761 tctaaactta gaggcagatg atgatggttt tgtcggaaat cctaagactg ttatgagatt
     5821 aactgaaaca actaaggatg atatgaaact tttaattgat agcgagtatg tgctattgtt
     5881 tgatactgga gtagtagtta taacagattg gacagagcat aacgctatca gaaaagacag
     5941 aaaaaaagat actagattta caaaagaaat gcaacaaata atattggtag acggtgggaa
     6001 atatttttgg ttgtcagata gtcaaccaaa cgacaaccaa tcggcaacca ttaatacacc
     6061 aaatgggtgc gtaggagagg ataggagagg taaggatagt ataggtaagg agagagtagt
     6121 agaagtagat aaagaacaaa atcaatcacc tactccccct ccttttaatc aagactttgt
     6181 aaatctctat aaatcttttg aggctgaaac tggtaaagcc ttatcgccaa tacagatgca
     6241 agaattgcag tacatgctag aagattttag cgctgatgtt attcatgagg cattgagaga
     6301 ggcagtaggt caaggtaagg ctaattttgc ttatattcaa gctattctca agcgatggaa
     6361 acaggataac ctattgacgg tagagcttat tagaaataat aaggcagcgc gtgaggttaa
     6421 gaaacagcaa aataattcaa aaccagaacc acaaacccgt gaagaatggt tggcgaactg
     6481 gtcagaagaa aacccatttt agaaaaagga gttacacatg ctaacacaag ctgaatttat
     6541 cgcaaacaca aagacactag aagagacttg ccccattcat ggcatcccgt taatgcaact
     6601 tgatagagtt gttaaaattg ctgaagaaga taaaccacgg aagctttctc cgatttgtcc
     6661 agaatgtata aaagagcaaa atgaaaagca agtgcaggaa gaggtagaaa aacacttgaa
     6721 cgcaggtatt tatcagaaaa cctataacgt gcttatgcgt gacagtacca tcccagaaga
     6781 cttaaaagcg gcatcttttg ataatttcat tattgagaca ccagaggaaa agcagatgtt
     6841 agattttgta aaaaatcaaa ctcaaaaata tcttgatggc atgaatggaa atactttgct
     6901 aacaggtact acaggggttg gaaaaactca tctagctatt tctcttgcca aagagctaaa
     6961 cgaggcgtat agagccaaag gagagcctaa aagtgtattg tttattaacc ttactgagat
     7021 actaagagaa atccgagaga gttttaagtt tactagcaaa gagggttatt attcacggat
     7081 gctaaaagag gttgattatc tgattttgga tgatttaggg gtcaaacttg gcaacgcctc
     7141 gggtcaatcc aagtcagcat gggaggaaga gtttattttt gatgtactta gtcatcggaa
     7201 taatactatc atcacaacta acctaagtaa tgatgagata gcaaaccttt atagcgaacg
     7261 tgttgcaagt cgtgtgcgta caggcttaga tggtaactat ttcaaagtat ttagtatcaa
     7321 agataagcgt tactcaatca ataagctaaa gagtaatatg tgataatgaa ttaacttaca
     7381 aacctaaaca aaaatagaca cttttcatgg ggagagaaat cgccccaatt tataagtaat
     7441 agccatgctt ttttctacga ccaaataaat caagcgcggc aaactaatca atttgaaaac
     7501 aactaaaaga accctaccta
//
LOCUS       DASFSW010000095         7224 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.095, whole genome shotgun sequence.
ACCESSION   DASFSW010000095 DASFSW010000000
VERSION     DASFSW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.095"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..578
                     /locus_tag="U1099_001548"
     CDS             <1..578
                     /locus_tag="U1099_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEM4867861.1"
                     /translation="GAVKVATVGDSLTYGYGLENREKDAYPCILAEKLGHHYQVSNYG
                     LSGRSLQSTADFPYFKEKNAQLSLESEADIVIIMIGSNDSRGPYWNRERFIREYREMA
                     EQYMDLPSQPDVYLVIPPYVPTSRFGLNNQIIEDELQKIIPAIGNELGLPVINLYTVT
                     EGHLEYYSDGLHLTPLGNQVIAEEIYQHLRH"
     gene            621..1163
                     /gene="lepB"
                     /locus_tag="U1099_001549"
     CDS             621..1163
                     /gene="lepB"
                     /locus_tag="U1099_001549"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM4867862.1"
                     /translation="MVKRDLIKQISLLVLLILGIIGLRFWLLEPVTITPEMANSYLKE
                     NDFIMTVRNVRPIHGDFILYNHEGKEYVSRVIALENETVTYMDDVLYRNDIIITENYL
                     KTPHSQESYTDDFTLETLTNGKYNVIPEGHYLVLNDVRTNQQDSRSFGLISSEAIVGR
                     LTFRISPLSEFGFIKTGLVQ"
     gene            complement(1213..2469)
                     /locus_tag="U1099_001550"
     CDS             complement(1213..2469)
                     /locus_tag="U1099_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM4867863.1"
                     /translation="MEQLYHTTELIGIKDKNIKILFILKHQTHLEIRAKLDYDSPGCP
                     HCQGKCIKYDFQKSSKIPILDCQGFPTLLLLKKRRFQCKSCQKVTVAETALVKKNCQI
                     SLPIWEKVTQLHTENMTNIAIARRLHISVSVVQRKLAQFQFEHDFTKLPKVLSWDEFS
                     RNKGKLAFIAQNFETRSIVTILDNNRQTTIKNYFYKYPRAVRETVEVVTVDMSGSYIP
                     ITKKLFPRAKIVLDRFHIIQHLSRAMMTTRIDIMKTFDTRSLPYRSMKNHWRILQKDS
                     RKLSLNRFFSRTFGQTVTPREVVQKTLNFSEELKFYYELYQILLFHFQEMNSKYFFEL
                     LEDNLDLVNPAFKTVFKTFLKYKTYITNAMELPYSNAKLEATNKLIKDIKRQAFGFRN
                     FTNFKTKIYIALNIKNERTNFVLSRC"
     gene            2776..4587
                     /gene="glmS"
                     /locus_tag="U1099_001551"
     CDS             2776..4587
                     /gene="glmS"
                     /locus_tag="U1099_001551"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEM4867864.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVTGGEQAHL
                     VKAVGRIAELSAKVGDKTEGTTGIGHTRWATHGKPTENNAHPHTSQTAGHILVHNGVI
                     ENYAEIKEEYLAGHDLKGQTDTEIAVHLIGQFAEEGLSTLEAFKKALKIIQGSYAFAL
                     IDATDADTIYVAKNKSPLLIGLGDGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKDS
                     VEVMDYDGNEIERGSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLISAYTNEAGQ
                     VTVDADIIKAVQEADRIYILAAGTSYHAGFASKDFLEKLTDTPVELGISSEWGYNMPL
                     LSKKPLFVMISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREATYTMLLHAGP
                     EIAVASTKAYTAQIATLAILAKAVGDANGNAYAKEFDLVHELSIVAQSIEASLSEKDV
                     IAEKVEKLLSTTRNAFYIGRGSDYYVSMEASLKLKEISYIQCEGFAAGELKHGTISLI
                     EDGVPVLALISNHPHLASHTRGNIQEVVARGANVLTIVDEAVAKEDDDITVTTVHPFL
                     SAIAMVVPTQLIAYYATLQRGLDVDKPRNLAKSVTVE"
     gene            4761..5402
                     /locus_tag="U1099_001552"
     CDS             4761..5402
                     /locus_tag="U1099_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM4867865.1"
                     /translation="MDYVLEVLPSLLNGAAVSLQVFFLVLILSLPLGAVFAFLMQIKF
                     KPLQWLLHFYVLIMRGTPLLLQLIFVYYVLPSVGIIFDRMPAVILAFTLNYAAYFSEI
                     FRGGIEAIPKGQYEAAKVLKFTPVQTIRYIVLPQVVKIVLPSVFNEVMTLVKDTSLVY
                     ALGVSDLLLASRTAANRDASLAPMFIAGAIYLLMIGAVTLISKQVEKKFDYYR"
     gene            5412..6044
                     /locus_tag="U1099_001553"
     CDS             5412..6044
                     /locus_tag="U1099_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM4867866.1"
                     /translation="MLELRNLSKRFEDKQIFSNYDLVIPEGKIVAIVGQSGGGKTTLL
                     RMLAGLETIDSGTLMYNGQELPLEELGKRHLLGFVFQDFQLFPHLSVLENLVLSPMKT
                     QNMSRSEAEDKALKLLDTLGLANHATAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRKEVEKLILENRATGITQIVVTHDMQFAENIADEIIKIEPKH"
     gene            6071..6901
                     /locus_tag="U1099_001554"
     CDS             6071..6901
                     /locus_tag="U1099_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM4867867.1"
                     /translation="MKKMMLGALALVTSLTLAACGSNDSATKSDNWTAYESEKSVTIG
                     FDKTFVPMGFEQTDGSYTGFDIDLANAVFEKYGITVKWQPIDWDLKETELNNGNIDLI
                     WNGYSITDERKEKVLFTNPYMDNQQVLVTKKSSNISQVSDMKDKILGAQAGSSGYSVF
                     ESQPAILKDIVQNNDASQYATFNEALIDLKNDRIDGLLIDRVYANYYLQQEGIIADYN
                     IIDAGFENEAFAVGARKSDTTLVENINKAFAELYKEGKFQEISQKWFGEDVATDAVKK
                     "
BASE COUNT         2141 a         1250 c         1600 g         2233 t
ORIGIN      
        1 gtggggccgt aaaagttgca acagtcggag atagtttgac ttatggatat ggcttggaga
       61 atcgggaaaa agatgcctat ccttgtattc tagctgagaa acttgggcat cactatcaag
      121 tttcaaatta tggattgagt gggcgttcac ttcagtcaac tgcagatttt ccttacttta
      181 aagagaagaa tgcccaactt tcacttgaga gtgaagcaga tattgtcatc atcatgattg
      241 gtagtaacga tagccgagga ccatattgga atagggagag gtttattcga gagtataggg
      301 aaatggcaga gcagtatatg gatttaccaa gccagccgga tgtttattta gtcatcccgc
      361 cttacgtacc aaccagtcgt tttggattga ataatcaaat tattgaagat gaattacaaa
      421 aaattatccc agctatcgga aacgaacttg gtttaccagt tattaacctt tacactgtga
      481 cggagggaca tcttgaatac tatagcgatg gtcttcattt gacaccgtta ggaaatcaag
      541 ttattgctga agagatttac cagcatctca gacactaatg gctctaaatt gtggtataat
      601 actagtgaaa tggagagatt atggtaaaaa gagatttaat aaaacaaatc agtttattgg
      661 tcttattgat tttgggaatc attggcctac gattttggtt acttgagcca gtcacgatta
      721 cccctgagat ggcaaatagt tatttgaagg aaaatgactt tatcatgaca gtgcgaaatg
      781 tccgcccgat tcatggagat tttattttgt ataaccatga gggaaaagag tatgtgagtc
      841 gtgtgattgc attggaaaac gagacggtta cctatatgga cgatgttctc tatcgtaatg
      901 atattattat tacagagaac tatttaaaaa cgcctcattc acaagaaagt tatacagatg
      961 actttacatt ggaaaccttg acaaatggaa agtacaacgt cattccagag gggcactatt
     1021 tggtgttgaa tgatgtacga acgaatcaac aggatagtcg tagttttggt ttgatttcta
     1081 gtgaggctat tgttggtaga ctgacttttc ggattagtcc actttcagaa tttggtttta
     1141 ttaagacagg gctggttcag tagaatcagc tttttttagg ctctttgtca actgtagtgg
     1201 gtagacgaaa agctaacacc tagagaggac gaaattcgtt ctctcatttt tgatgtttaa
     1261 agcaatatat atctttgttt taaagttcgt aaagttccta aagccgaacg cttgcctctt
     1321 gatgtctttg atgagtttat tggtcgcttc cagcttagcg ttggaatatg gaagctccat
     1381 ggcgttcgtg atgtacgttt tatactttag aaaagtttta aaaacagttt taaaggcagg
     1441 attgacaaga tccagattgt cttctagaag ctcgaagaaa tatttcgagt tcatctcttg
     1501 gaaatggaag agaaggatct gatagagttc gtaatagaat ttcagttcct cagagaaatt
     1561 caatgtcttc tggacaacct ctctcggtgt tactgtttgc ccaaaagtgc gagaaaagaa
     1621 gcgattcaga gacagttttc gactatcttt ttggagaata cgccagtgat ttttcatgga
     1681 tcgataaggt agggaacgcg tatcgaaagt cttcataata tcaattctag tcgtcatcat
     1741 agcacggctc aggtgttgga taatgtgaaa tctatcaagg acaattttag ctcttggaaa
     1801 tagttttttg gtgatgggaa tatagctacc cgacatgtcc actgttacga cctcgacagt
     1861 ttctctgacc gccctaggat acttgtagaa atagttcttg atggtggttt ggcggttgtt
     1921 gtcaagaatg gtcacaatcg atctcgtctc aaaattctga gcaataaagg cgagttttcc
     1981 cttgttccga ctaaattcat cccaactcaa gacttttggt aatttcgtaa aatcatgctc
     2041 aaattgaaac tgagccagtt tcctctggac gaccgatacg gagatatgta atctcctagc
     2101 gatggctata ttagtcatgt tttctgtatg gagttgtgtc actttctccc aaataggtag
     2161 ggaaatctga cagtttttct tgactagagc tgtctcagct acagtcactt tttggcaaga
     2221 tttgcattga aaccgccgtt tcttaagcaa aaggagggtt ggaaagccct ggcaatccaa
     2281 aatcggaatc tttgacgact tttgaaagtc gtacttgata cactttcctt ggcaatgagg
     2341 acaaccaggg ctgtcgtaat ctagctttgc cctgatttct agatgggtct gatgtttcaa
     2401 aatgaagaga attttgatgt ttttgtcttt gattccaatt agttctgtgg tatgataaag
     2461 ttgttccata tgagtctttc taatgtgagt gtggttgctt ttcattatag gtcatatggg
     2521 actttttgta tatactcaaa aagcctccat aatctctaca gtggatttac ccactacaga
     2581 aattatagag ccttttttta attgtgttga gatgactgga actgtatttt gaaattagac
     2641 tagaccaatt aaaatgttga caaggataaa aacttgttat atactgattt cactggtctt
     2701 tttagataca aattagacta gaccaatttt taaagaaatg aaagccgatg ttgacaaaca
     2761 tcgtaggtga tatgtatgtg tggaatcgtt ggtgttgtag gaaatacaaa cgcaactgat
     2821 attttgattc aaggacttga aaaattagaa taccgtggtt atgattcagc aggtattttt
     2881 gtgacgggtg gtgagcaagc tcatcttgtg aaagctgttg gtcgcattgc agaattgtct
     2941 gcaaaagttg gcgataagac tgaagggaca acagggatcg gtcatactcg ttgggcgact
     3001 catggtaagc caactgagaa caatgctcac cctcacactt ctcaaactgc tggacatatc
     3061 cttgttcaca acggtgtgat tgaaaactac gcagaaatca aggaagagta tcttgcaggt
     3121 catgacttga aagggcagac agatacagaa attgctgtac acttgattgg tcaatttgca
     3181 gaagaaggtt tgtcaactct agaagccttt aaaaaagcat tgaagattat tcaaggttca
     3241 tatgcctttg ctttgattga tgctacggat gcagatacaa tctacgttgc caaaaataaa
     3301 tctccacttt tgattggtct tggtgatggc tataacatgg tctgctcaga tgctatggct
     3361 atgattcgtg agacaagtga atacatggaa attcacgata aggaattggt tatcgtgaaa
     3421 aaagatagtg ttgaagtcat ggactatgat ggaaatgaga ttgaacgtgg tagctataca
     3481 gctgagcttg atctgtcaga tattggtaag ggaacttatc catactacat gctcaaggaa
     3541 attgatgagc aaccaactgt tatgcgtaaa ttgattagcg cctatacaaa tgaagctggt
     3601 caggtaacag ttgacgcgga tattattaag gcagtacaag aagctgaccg catctatatc
     3661 ttggctgctg gaacttccta tcacgcagga tttgcctcaa aagacttctt ggaaaaattg
     3721 acagatacac cagtagagtt gggaatttca tctgagtggg gctacaatat gccgcttttg
     3781 agcaaaaagc cactctttgt catgattagc cagtcaggtg aaactgctga tagccgtcag
     3841 gtattggtta aagctaatga aatgggcatt ccaagtttga ctgtaaccaa cgttcctggt
     3901 tctaccctat ctcgtgaggc aacttacacc atgttgctcc atgcaggacc agaaattgcg
     3961 gtagcttcta caaaagccta cactgcacag attgcgactt tggcaatctt ggctaaggct
     4021 gtcggtgatg ccaatggcaa tgcctacgcg aaagaatttg acttggtgca tgaattgtcc
     4081 attgttgccc aatctatcga ggccagcttg tcagaaaaag atgtgattgc tgaaaaagtt
     4141 gaaaaactct tatcgactac tcgcaacgct ttctatatcg gtcgtggtag tgactactac
     4201 gtttctatgg aagcaagttt gaaattgaaa gagatctcat acatccaatg tgaaggcttt
     4261 gcggccggtg aattgaaaca cggcacaatt tccttgattg aagatggtgt accagtcttg
     4321 gccttgattt caaatcatcc gcaccttgct agccatactc gtggtaatat tcaagaagtt
     4381 gttgcgcgtg gtgcaaatgt cttaacaatc gtagatgaag cagtagctaa agaagatgat
     4441 gacatcactg ttacaactgt tcatccattc ttgtcagcga ttgctatggt ggttccgaca
     4501 cagttgatcg cttactatgc gacattgcaa cgcggtttgg atgttgataa accacgtaac
     4561 ttggctaagt ctgttactgt tgaataagaa gaaggaaggc gtggatttcc agccttcttt
     4621 ttgcactgtt gaaaaaatat acaaaatttt gttaaatttt gttaaatttt gttaaattta
     4681 ttaaaaattc tgaaaaattc tttgaaatta aggatatttt ttgatatact agaacctaat
     4741 cattctttta ggagagctct atggattatg ttttggaagt tctgcccagt ctactaaatg
     4801 gtgctgcagt ctccttgcaa gtattttttc tagtattgat attatccttg ccgttaggag
     4861 ctgttttcgc ttttttaatg cagattaaat ttaaaccctt acaatggtta ttgcatttct
     4921 atgtattaat tatgcgagga acccccttgc ttttacaatt aatttttgtt tattacgtct
     4981 taccaagtgt gggtattata tttgatcgaa tgcctgctgt gattctggcc tttacgctca
     5041 actatgcggc ttatttctct gaaattttcc gtggtggtat tgaagctatt ccaaaaggtc
     5101 agtacgaagc tgctaaggta ttgaaattta cccctgttca gaccattcgc tacattgtct
     5161 taccacaggt tgtgaaaatt gttctgccaa gtgttttcaa tgaagtcatg actttggtaa
     5221 aggatacttc tttggtctat gcacttgggg ttagtgattt gttgttggct agtcgtactg
     5281 cggccaatcg tgatgccagc ttggcgccta tgtttatcgc aggtgcaatc tacctattga
     5341 tgatcggtgc ggtaacactg atttctaaac aagtagaaaa gaaatttgat tattatagat
     5401 aggaggaagc catgttagag ttacgcaatt tgtcaaaacg ctttgaagat aaacagattt
     5461 tctccaacta tgatttggtc attccagaag gaaaaattgt tgccatcgtt ggtcaatctg
     5521 gcggtggtaa gacaactctt ttacggatgt tggctggttt agagactatt gattcaggaa
     5581 ctttgatgta taatggtcaa gaactgccat tagaggagtt gggaaaacga catttgctgg
     5641 gctttgtctt ccaggacttc cagcttttcc cgcacctatc cgtattggaa aacttggttc
     5701 tctctcctat gaaaacgcaa aatatgtcgc gttcagaggc ggaagacaag gcccttaaac
     5761 tcttggatac tcttggactt gctaaccacg caactgccta cccattttca ttatcgggtg
     5821 gacagaaaca acgtgtggcc ttggcgcgtg cgatgatgat tgatcctgag attatcggct
     5881 atgatgaacc aacttcggcc ctagaccctg aattaagaaa agaagttgag aaactgattc
     5941 ttgaaaatag ggctactgga atcacgcaaa ttgtggtcac ccatgatatg cagtttgctg
     6001 agaatatcgc agatgaaatc atcaaaattg aaccgaaaca ctaagaaaag gaacaaatat
     6061 gatgatgaat attaaaaaaa tgatgcttgg agctcttgct cttgtaactt ctttgactct
     6121 tgctgcttgt ggctccaatg attcggctac taagtctgat aattggacag cctacgagtc
     6181 tgaaaaatcc gttacgattg gctttgataa gacctttgtc ccaatgggat ttgaacagac
     6241 agatggttct tacacaggtt tcgatattga cttggctaat gcagtctttg aaaaatatgg
     6301 aattactgta aaatggcaac caattgactg ggacttgaaa gaaactgaat tgaataacgg
     6361 gaacattgat ttaatttgga atggttattc tattacagat gagcgtaagg aaaaagttct
     6421 tttcacaaat ccctacatgg ataaccaaca agttcttgta actaaaaaat cttccaatat
     6481 tagccaagta tctgatatga aggataagat tttaggtgcg caagcaggtt catctggtta
     6541 ttcagtattt gaatcacagc cagctatttt gaaagatatt gttcaaaata acgatgcaag
     6601 tcagtatgcg accttcaatg aagccttgat tgacttgaaa aacgatcgta ttgatggcct
     6661 tttgattgac cgtgtgtatg caaattatta cttacaacaa gagggaatta tcgcagatta
     6721 caacatcatt gatgcaggtt ttgaaaatga agccttcgca gtcggtgctc gtaaatcaga
     6781 tacgacactc gtagaaaaca ttaataaggc gtttgctgaa ttatataaag aaggtaaatt
     6841 ccaagaaatc tcccaaaaat ggtttggtga ggatgttgca accgatgcag taaaaaaata
     6901 ataatgtaag aaaaagtgac tgaacaagac atccatgatg gttacgctct catccacaga
     6961 ataatgaaaa gaactcgtga aaacggtggg ggtgtacacc aatatcatta acttaagatt
     7021 ctacgaggct gagcaaaaag ctcagcgcca cttctcggag ttcgtgtcaa catctcagcg
     7081 cagtggttga ttggctccaa cagtcactat tctgactgtt ggagctatgc gggggtggga
     7141 gtttaaaagg tctgggagac cttttaaagt gggagatata agctgacggc agtcagctca
     7201 cataaattga tgtctgggga taga
//
LOCUS       DASFSW010000096         7222 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.096, whole genome shotgun sequence.
ACCESSION   DASFSW010000096 DASFSW010000000
VERSION     DASFSW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.096"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(44..676)
                     /locus_tag="U1099_001555"
     CDS             complement(44..676)
                     /locus_tag="U1099_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867868.1"
                     /translation="MFSIQSIQKKFGKRSLFSDVTLDLQAGKVYALIGASGSGKTTLL
                     NILAKLESYDAGDILYKGKDLKKLKPHLFFREELGYLFQNFGLLESQTIKENLDLGLV
                     GQKLSKDERKSRQEAALDAVGLSYLDLSQPIYELSGGEAQRVALAKVILKNPPLILAD
                     EPTAALDPKSSQEIMDILLSLKSPERLIVIATHNPLIWERADLIIKMEDL"
     gene            complement(678..2660)
                     /locus_tag="U1099_001556"
     CDS             complement(678..2660)
                     /locus_tag="U1099_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEM4867869.1"
                     /translation="MKGKFVLVSTIILAVFMIWLGVSQKETMLVHYYPSVTSLSVSED
                     VTYSDVRQRLEEYSQQTDSVIARRVIEPSTSGGRTFSYDNFSQSSLPRGLEEFQASEK
                     VESALLTKYFIFQGKATVEELRSLLVSLGFDEVQIRKPSTIATLLAFLTQGGQFLAVL
                     VFLITYMALVVVANVRQLRTAGIRLIAGDSRWHLFLLSLQENAKEIALTIPFAVLPAV
                     GLAYLIGLDSYSVYYLVAALVGYHFLLGLIALFFAATFTLAIRIYHFLPLLKGKMPLQ
                     GILTIMVMGQMLALLVVSLGMAQTVYYSGIWQEYQAGARQWEKEEDYYSLAWNIAADG
                     HSGLNSPENWYPLLKQALEKDGALFVKSNLNAYLMGSQLEDGTSLDSYHPAGNTLYVS
                     PNYLQIQDVDLSDEALPSLQEGEFQLLLPEKLRPESDIYLHLYQDYINRMVRPANQAS
                     SATIKGKLAYLKDGQKQFIYNHRSSQHVQYLTDPILVVLAPSSLGKESADFWLNEVDS
                     SILFKNRDKLLRELKARNLFQFVNEVRSSSSLFTELLDRIRIETISTSMGAILGIVTS
                     IILFNTMNLLYFEEFKRDIFIKEIAGMDFLGIHKKYLTFQLLSLLLALGASMVVTGHM
                     FISSISFALFMVNALCLLRWQARKDGTWNIRILKGA"
     gene            complement(2680..4665)
                     /locus_tag="U1099_001557"
     CDS             complement(2680..4665)
                     /locus_tag="U1099_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEM4867870.1"
                     /translation="MKKVFTYLSTFSVCLFCIWLVSLQLDSLIYHYYPSVSILSRGEE
                     VTYEEVSDALTNLAEKENSLIAKTITETDDSGRTTTSYDVFGKGELPQGLVKADRLVA
                     SQANLAENYYVVKGNLDIKVVENVLQSLGLVDSYIYRPTLLNSLSLFLGRGAPSLGLI
                     IFILTNLALTVVGQTVYLRSAGIEMLSGVRSWQIASRFLRVDGRDIALSTGGALVFGL
                     IAILILGWPMVYYRFVAIGIAFYACLLFFLSLSTSIGFMIALNISQLQKLIKGALPLK
                     AIFAILLVGQCLAVCIISIGASRTLTYVGVLHTLTIGEKAWQERQDVFSLSLNREAFI
                     ADEAMVQQQRMAWSHVILESYAKNNVMLVQHHLRHNTIKEHTIIESSKEIPLERVLYV
                     TPNYIQSEGGLCDFNQLEEIKQLGKGEIALLLPKSLQNDASVYQAYFEDMVGKLLADG
                     EKSISLYSNVYYISDEKRWFIYNHTPINYEQFLQAPLIVVLSPESFEETSYFWENALP
                     DFVFFKDKEMLEQLLEKYNLRNTIGSLLSSRQQYNTLRKNVQLEILMTLSPTILGIFT
                     SILLFNTMNLLYFETFKREIAIKRIAGMRFIELHGSYLGEQVGSALVGMGLAMFMTKS
                     VIVSLGVVVALLINSWMLLNKQAKKAEKIQLSVLNGR"
     gene            complement(5040..5333)
                     /locus_tag="U1099_001558"
     CDS             complement(5040..5333)
                     /locus_tag="U1099_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867871.1"
                     /translation="MDLNDYYTDIDPDFSFRELRRKIERYRIVMQARVLLDKRDTIKR
                     TGKTDIQKIASETKQTIQTMRGRLETSIKGSVRTGIEEAVDKHSSLYDTITQS"
     gene            complement(5320..5709)
                     /locus_tag="U1099_001559"
     CDS             complement(5320..5709)
                     /locus_tag="U1099_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867872.1"
                     /translation="MESDVIWERIRKREQELFDLEDDYNQEKNKIEARQEDLEQRQNA
                     LKLLIEREQEEMRCFLSRHSLDYDAALSFFQELDQLQEESFYQYRQEMDQLFQQEERL
                     SQQYRTDLYRLEDTISQLRRDYSNGLE"
     gene            complement(5694..6908)
                     /locus_tag="U1099_001560"
     CDS             complement(5694..6908)
                     /locus_tag="U1099_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM4867873.1"
                     /translation="MISDKDYRDISKVVYDVDLLKAPKTPLIQDTPVVNGSFIIIEPP
                     VDAGNGMQAMAVAPIKGYDTDNKPIPDTSQVVIAYAGTNDGDIKDWATDISMIGGGNT
                     ATFTVPGQNVPYISQSITAVAFADQIVQRYPDATITITGHSLGGSLAMYVALKKDLPA
                     ITFNGPDIHNMISEDEIEYMQQHRAQFRNYRNKYDFLGDITGNKTKSAIYPAVKNIPG
                     DAIADHMLNAWKFNKYGQLIDANGQVVSTFTGAPLEDTRLAMKRWKKQKTALSKGGLS
                     RSEEIFLDSEQARTISAGIASVADAGQMSIFAHCDDYVHQAEELWNSIDFHSYSALSY
                     WEVCALFSEYGVSQETIVREIEETMDIYRQRAEIATEKFTILDTQIIQAINTMLETDK
                     RLAGDFEKWNQM"
     gene            complement(6905..>7222)
                     /locus_tag="U1099_001561"
     CDS             complement(6905..>7222)
                     /locus_tag="U1099_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867874.1"
                     /translation="RMSEEEKLQAQIRKEQERMVLYAVNHYEGIEKIEFVNFEKDNKT
                     GTWDSDAIINDKFHVTFVSWGEDDITINGGKSQTGDYLVPKVATTVTEISNIQVKYYK
                     ELP"
BASE COUNT         2121 a         1654 c         1266 g         2181 t
ORIGIN      
        1 aagttgacaa cggataattt tgattttcga agagtatgaa tttttacaag tcttccattt
       61 ttatgataag gtctgctcgc tcccaaatca aaggattatg ggtcgcaatc acaattaacc
      121 gttctgggct cttcaaggat aacaaaatat ccataatttc ttgggagctc ttaggatcca
      181 aggcagccgt cggctcatct gctaaaatca atggcggatt tttcaggatg actttagcaa
      241 gagctacccg ctgggcttcc cctcctgaga gttcatagat aggctgggac aggtccagat
      301 aagacagacc aactgcatca agtgccgctt cctgcctcga tttcctttcg tctttggaca
      361 atttctgacc aaccagtccc aaatccagat tttctttgat tgtctgactt tctaaaagac
      421 caaaattctg aaagagatag cctaattcct ccctaaaaaa gagatggggc ttcaactttt
      481 tcaaatcctt ccccttgtag agaatgtcac ctgcatcata ggactccaac ttggctaaaa
      541 tattcagcaa ggtcgttttt ccgctaccac tggcacctat caaggcatag acctttccag
      601 cctgcaagtc tagcgttaca tcagaaaaca aactgcgttt cccaaatttc ttttgaattg
      661 attggataga aaacatacta ggctcctttc aaaatacgga tattccaagt tccgtctttc
      721 ctcgcctgcc acctcaataa acagagggca ttgaccataa acaaggcaaa actgatacta
      781 cttatgaaca tgtgacctgt cactaccatg cttgctccaa gagccaacaa gagggaaagc
      841 agttggaacg ttaggtattt cttatggatt cccaagaaat ccatccctgc aatttctttg
      901 ataaaaatat cacgtttaaa ctcttcgaag tataatagat tcatggtatt aaacaggata
      961 attgatgtca caatccctaa aatagcaccc atagatgttg aaatggtctc tatccgaata
     1021 cggtctaaaa gttctgtaaa gagggaggaa ctacttctca cttcattgac aaactgaaac
     1081 agatttcttg ccttcaattc gcgcaaaagt ttatccctgt ttttaaataa aatactgcta
     1141 tctacttcat tcagccaaaa gtcagcagat tcttttccca gacttgacgg tgccagcaca
     1201 actaaaatag ggtctgtcag gtattgcaca tgttgactag aacgatgatt gtagataaat
     1261 tgcttctgcc catcttttaa ataggcaagt tttcccttga tggtagctga gcttgcttga
     1321 ttggcaggtc taaccatacg attgatatag tcttggtaaa gatggagata aatatcgctt
     1381 tcagggcgca atttttcagg taagagaagt tgaaattctc cttcttgtag agaaggtaat
     1441 gcttcgtccg acaagtctac atcctgtatc tgcaaataat ttggactaac gtagagggtg
     1501 ttgcctgctg gatggtagct atcaaggctt gtcccatctt ccagttggga gcccattaaa
     1561 taggcattca aattactttt gacaaagagc gcgccgtctt tctccaaagc ttgctttagt
     1621 aaagggtacc aattttcagg cgaatttagt cctgagtgac catccgcagc aatgttccaa
     1681 gccaaactat agtagtcctc ttctttctcc cactgccggg cacccgcttg gtattcttgc
     1741 caaatccctg aataatagac ggtttgagcc atgcccaatg acaccaccag tagagccagc
     1801 atttgcccca taaccataat ggtcaggatt ccttgcaggg gcattttccc ttttaacaag
     1861 ggtaaaaagt gatagattcg aatggccaag gtaaatgtag cagcgaaaaa aagggcaatc
     1921 aaaccaagca agaaatgata gcctactaga gctgcgacca gatagtagac agaatagcta
     1981 tctaatccaa tgaggtaggc cagtccaact gctggaagaa ccgcaaatgg gatagttaga
     2041 gctatctctt tcgcattttc ctgtagagat agtagaaata aatgccaacg tgaatctcct
     2101 gcgatcaaac ggatacctgc tgtgcgcaat tgccttacat tggcaaccac taccaaagcc
     2161 atataggtaa taagaaagac cagtacggct aagaattgac caccttgtgt cagaaatgcg
     2221 agtaaagtag caatagtcga aggcttccga atctgaacct cgtcaaatcc aagcgaaaca
     2281 agaagagacc gaagctcctc tactgtcgct ttcccttgga aaataaaata tttggttaaa
     2341 agcgcagact ccaccttttc tgatgcctga aattcctcca gtcctcttgg taaggaagac
     2401 tgactaaaat tatcataaga aaaggtacgt ccacctgatg tacttggctc aatcacccgc
     2461 ctggcaatca cactatccgt ctgttgagaa tattcctcta atcgttgtct tacatctgag
     2521 taagtcacgt cctcagatac cgacaagctg gtcacagaag gataataatg aactagcata
     2581 gtctcctttt ggctgacgcc caaccaaatc ataaagacgg ctagaatgat cgtcgataca
     2641 agtacaaatt ttcctttcat tctaacttca cctcattaac tatctgccat ttaatacact
     2701 caactggatt ttttctgcct ttttcgcttg tttgttcagt aacatccaac tatttatcaa
     2761 cagagccaca actactccta ggctaacaat aactgacttt gtcataaaca ttgccaaacc
     2821 catcccaaca agagcggagc cgacttgttc tcctagataa gaaccatgca attcgataaa
     2881 acgcatccct gctatccgct tgatggctat ttctcttttg aaggtctcaa agtatagaag
     2941 attcatcgta ttgaacaata gtatggaagt gaatattcca aggattgtcg gagatagggt
     3001 cataagaatt tcaagttgaa cattttttct cagggtgttg tactgttgcc tgctagataa
     3061 caagctacct attgtgtttc gtaaattgta tttctccaac agctgttcca gcatctcttt
     3121 atctttgaaa aatacaaaat ctggtaaggc attttcccaa aaataggacg tttcttcaaa
     3181 actttcaggt gatagaacaa caatcaacgg agcctgtaaa aattgctcat aatttatcgg
     3241 agtatgattg taaatgaacc atcgcttctc gtctgaaatg taataaacgt ttgaatacaa
     3301 acttatggat ttttctccat ccgcaagcaa tttccctacc atgtcctcaa aatacgcttg
     3361 gtaaacactc gcatcatttt gcaaactctt tggtaataat aatgcaattt ctcctttacc
     3421 taattgcttt atctcttcca gctgattgaa atcgcacagt ccaccttctg attgtatata
     3481 attcggcgtc acataaagta ccctttctag aggaatttcc ttgctggatt caatgatggt
     3541 atgttctttt attgtgttat gccgtaaatg gtgctggact aacattacat tatttttcgc
     3601 atatgattct aaaattacat gagaccaagc cattctttgt tgctggacca tcgcttcatc
     3661 agctatgaat gcttcacgat tcaaagacag actaaaaaca tcttgtcttt cttgccaagc
     3721 cttttctcct atcgttagtg tatggagaac tccaacgtat gtcaacgttc gactagcacc
     3781 aatagagatg atgcaaaccg ctaaacactg acctactagt aaaatagcaa aaatcgcctt
     3841 gagaggcaaa gctcccttaa ttagcttttg taactgagaa atgtttaatg caatcatgaa
     3901 tccaatactt gtcgataaag ataggaaaaa aagcaaacaa gcataaaagg cgataccgat
     3961 tgctacaaaa cgataatata ccattggcca acccaaaatt aaaatagcaa tcaatccaaa
     4021 aacaagcgct cctcctgtag acaatgctat atctcttcca tccactctta aaaaacgact
     4081 agcaatctgc caactcctca cccctgaaag catctctata cctgccgacc gcaagtagac
     4141 agtctgccca actactgtca atgcaagatt ggtgagtata aagataataa ggcctaagct
     4201 cggagctccc ctaccaagaa atagacttaa actgtttaat agggtcggtc tataaatata
     4261 gctatccacc aatcccagtg actgtaagac gttttctacc actttgatgt ccagattccc
     4321 ttttacaaca tagtaatttt cagctaagtt ggcctgacta gctactaacc tatccgcttt
     4381 gaccaagccc tgtggaagct cccccttccc aaaaacatca taactggtag tagttcgccc
     4441 actatcatct gtttctgtta ttgtcttagc aattagacta ttttcctttt cagctaaatt
     4501 tgtcagggca tccgacactt cttcataggt cacttcttcc cctcgactta atatggaaac
     4561 cgatggatag taatgataaa ttaaagaatc gagttgtaga ctcactaacc atatacagaa
     4621 gagacagacg ctaaaagtag ataaataagt aaatactttt ttcataacga acctcatttg
     4681 cacaaaaagg aggtggactc caccacctcc ctatcattag aacccgtaac caaaatcaac
     4741 atgttttcca aagtttattg tatcacttcg ctattatctc tatcttaaca tataaacaaa
     4801 acaaaaacaa ggttgcttgc attcttacca tgccgtacgc atgaagaaaa tgcgtataac
     4861 catttcctag acatctgcat tttcaaagcg ttttcatgaa accaatactt caaccaggat
     4921 cgtctttcat ttccaaagct ataaacagtt ttctaccaaa aacggatgcc aacagcaaga
     4981 aaatagtttt gaaaacgaaa atttctttat tcgcttgtta tgcgtaacta tatctcagct
     5041 taactttggg tgattgtatc atacaagctg ctgtgtttat ccacggcttc ttcaatccct
     5101 gtacgtacac tccctttgat actggtttcc aaccgtcctc tcatcgtttg aatcgtttgc
     5161 ttcgtttcgc tggctatctt ctgaatgtct gtcttccctg ttctttttat agtgtcgcgt
     5221 ttatccaaca agactctagc ctgcattact atgcgatagc gctctatttt ccgccgtagt
     5281 tctcggaaag agaaatcagg atctatatcg gtatagtaat cattcaagtc catttgaata
     5341 gtccctcctt aattggctaa tagtgtcttc taagcggtag aggtctgtcc gatattgctg
     5401 ggacagcctc tcctcctgtt gaaacagttg atccatctct tgacggtact gataaaagga
     5461 ttcttcttgt aattgatcca actcttgaaa aaaggacaag gccgcatcat aatccaagga
     5521 atgacgactg agaaagcaac gcatctcttc ctgttctctt tctatcaaga gttttaaagc
     5581 attctgacgc tgttctaggt cttcttgacg tgcttcaatc ttatttttct cttggttata
     5641 gtcatcctcc agatcaaata attcttgctc tcgcttacgg attctttccc atattacatc
     5701 tgattccatt tctcaaaatc tcctgctagt ctcttatctg tttccagcat tgtgttgatt
     5761 gcttgaataa tttgagtgtc caagatggtg aacttttctg tagctatctc tgccctctgc
     5821 cgatatatat ccatagtctc ttcaatctct ctgacaatgg tttcctgact aacaccatac
     5881 tctgagaaaa gggcacaaac ttcccaatag ctgagagctg aataggaatg aaaatcaatc
     5941 gaattccaca actcctctgc ttggtgtaca tagtcatcgc aatgggcaaa aatactcatt
     6001 tgccctgcat ctgcaacact agcaatccct gctgagatgg ttctggcctg ctcactgtcc
     6061 aaaaaaatct cctcactacg acttaggcca cctttagata aggctgtttt ctgctttttc
     6121 cagcgtttca tagccaaacg agtatcttcc aaaggagcac cagtaaaggt tgataccacc
     6181 tgaccgttgg catcaatcag ctgaccatat ttgttaaact tccaagcatt tagcatgtga
     6241 tcagctatag catcccctgg gatattcttc accgctggat aaatagctga tttcgtctta
     6301 tttcccgtaa tatctccaag gaagtcatac ttatttcgat agttacgaaa ctgtgctcta
     6361 tgctgctgca tatactctat ttcatcttct gaaatcatat tgtgaatgtc gggaccgtta
     6421 aaggtaatag caggaagatc tttcttcaaa gcaacataca tcgccagaga gcctcctaac
     6481 gaatgccctg tgattgtgat ggttgcatcg ggatagcgtt gtactatctg atctgcaaaa
     6541 gctaccgcag ttatagactg agatatatag gggacattct gaccgggaac ggtaaaagta
     6601 gccgtattcc ctccgcctat catagaaata tctgttgccc aatctttaat gtcgccatca
     6661 ttagtccctg cgtaggcaat aacgacttgg gaagtatctg gaatcggttt gttgtcagtg
     6721 tcgtaaccct tgatcggagc aaccgccatg gcctgcatac cgttgcccgc atcaacaggt
     6781 ggctcgatga tgataaaact accatttaca actggagtat cttgtattag tggagtttta
     6841 ggtgccttta ataggtctac atcataaacc actttagaaa tatctctata atctttatcc
     6901 gatatcatgg caactcctta tagtatttta cttgtatatt actaatttct gttactgtgg
     6961 ttgctacttt aggaactaag taatctcctg tttgagattt tccaccattt attgtaatat
     7021 catcttctcc ccaagataca aaagtaacat ggaatttgtc atttataatt gcatcactat
     7081 cccatgtccc tgtcttatta tccttttcaa aatttacaaa ctctatcttc tcaatccctt
     7141 catagtgatt gacggcgtac aaaaccatcc gctcttgttc ctttcggatt tgagcctgca
     7201 atttttcttc ctcactcata cg
//
LOCUS       DASFSW010000097         7205 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.097, whole genome shotgun sequence.
ACCESSION   DASFSW010000097 DASFSW010000000
VERSION     DASFSW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.097"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            51..938
                     /locus_tag="U1099_001562"
     CDS             51..938
                     /locus_tag="U1099_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEM4867875.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDLYMMGA
                     FMGYFLLNNLNFIADGGTRFFVALILAMVGTAILGVVIEFLAYRPLRNSTRIAALITA
                     IGVSFFLEYTMIKFFTADVKAFPQAIEAVTFNLGPVSVTNIQLIILGVSLALMVALQL
                     IVKRTKMGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLLGLYYNQIE
                     PLMGMTPGLKAFVAAVLGGIGIIPGAALGGFVIGIIETFTYVIEFDTFRDAIVYTVLI
                     IILLVRPSGILGKNVKEKV"
     gene            941..1888
                     /locus_tag="U1099_001563"
     CDS             941..1888
                     /locus_tag="U1099_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002914606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEM4867876.1"
                     /translation="MKQNLKVNAIWLAILLAGFGLIQGLVSAGILNFYYIQIVQQIGI
                     QIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAVGVLGKMMPTYGGFAIALIVGMLISG
                     AVALLVGIPTLRLKGDYLAIATLGVAEIIRILIINGGSVTNGAAGIMSIPLFTSWPLV
                     YIFVIITTLVTVNFLRSPMGRATISVREDEIAAESVGVNPTFVKVSAFVFGAMTAAIA
                     GGLHAGYVGTIVPKDFAFMTSVNVLIIIVLGGLGSITGTFVAAIVLGILNVFLKSYSD
                     ISMIIYSLALILLMIFRPGGLLGTKEFSVAALLKKKEVK"
     gene            1888..2652
                     /locus_tag="U1099_001564"
     CDS             1888..2652
                     /locus_tag="U1099_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867877.1"
                     /translation="MALLEVKDLTKNFGGLTAVGDVTMELHEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTISLAGTILNGKAPSKIASLGLGRTFQNIRLFKNMTVLENVLIG
                     LGNHGKSEVFASFFRLPAFYKNEEALKTKAIELLKIFDLDGDADTLAKNLPYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLIRKIKEEFGITIILIEHDMSLVMEV
                     TERIYVLEYGRLIAHGTPEEIRNNKRVIEAYLGGEA"
     gene            2652..3362
                     /locus_tag="U1099_001565"
     CDS             2652..3362
                     /locus_tag="U1099_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4867878.1"
                     /translation="MAMLEVKNLSVNYGVIEAVKDVSFEVNEGEVVTLIGANGAGKTS
                     ILRTISGLVRPSAGTISFLGNEIQKVPARKIVADGLSQVPEGRHVFAGLTVMENLEMG
                     AFLRNNREENQANLKKIFARFPRLEERKNQDAATLSGGEQQMLAMGRALMSQPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAIADRGYVLETGKVVLSG
                     TGKELLASEEVKKAYLGG"
     gene            3733..4488
                     /locus_tag="U1099_001566"
     CDS             3733..4488
                     /locus_tag="U1099_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEM4867879.1"
                     /translation="MIKVEEILSTLSPNQKVNYDKVFQKMASTWQEAGLRPKILMHVC
                     CAPCSTYTLEYLTQYADVTIYFANSNIHPKAEYQRRAAVTAQFVKEFNQKTGQSVGYL
                     EADYSPQEFFKKTQHLANEPEGGDRCKVCYDYRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINAAGIEVQKLYGTQYLPSDFKKNGGYQRSVQMCEEYDIYRQCYCGCVYGANA
                     QGVNLVQVKKEALEFLKTHRDYSHIPFKVLDKN"
     gene            4596..5186
                     /locus_tag="U1099_001567"
     CDS             4596..5186
                     /locus_tag="U1099_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867880.1"
                     /translation="MKLRKVSFILVTFVIGLLSLSSVKAETSMFVPVGQQNVPGVEVA
                     PFRTGSDSIHVHVGSFGYVATYINGERLKVEPVKHELKCPSYTTENCQTKEWYTSGER
                     ADGSGDYVVKLNKKLEEGDVVTLKFADDGNLYFGQLVYKSEKKRVEQTQKEQEDEYAD
                     ALFKRSIEEEENKTWRDRIKDTFQDAWWNFKGWWNS"
     gene            5336..6367
                     /locus_tag="U1099_001568"
     CDS             5336..6367
                     /locus_tag="U1099_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEM4867881.1"
                     /translation="MTVIEQIEKLKKEKLELLTVLCGEDIGQYQIAKDLLLRKLDFDA
                     ADLGFAYFDMSEADYHQVDLDLVSLPFFSDEKVVILDYFADLTTDKKRHLTDDELKQF
                     EVYLENPVETTCLVILAPGKLDSKRRLVKLLKRDGLVLEANPLKEMDLKNHFQKEIMR
                     LGLQMGGEVFQYLLEKSNFDFAEISKNLVFLQSYKGKETIEISDIDAAIPKTLQDNIF
                     DLTQLILQSKVDEARNLVRDLRLQGEEEVKLIAIMLTQFRTYLQVQILLEQGRGEQQI
                     VAELSTIMGRKVNPYQVKYALRDSRHLSIGFLKKVVKLLIDTDFQIKTGQFDKDYLFD
                     LLLLKIATA"
     gene            6480..7085
                     /locus_tag="U1099_001569"
     CDS             6480..7085
                     /locus_tag="U1099_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEM4867882.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLVALLSDVEQIPADIRQALINNGGGHLNHALFWELLSPEKTEISAELAAD
                     IDATFGSFDAFKEAFTAAATTRFGSGWAFLVVNKEGKLEVISTANQDTPIMQGLKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEVINWDKVNELYKAAK"
BASE COUNT         2072 a         1258 c         1644 g         2231 t
ORIGIN      
        1 tgtaacaatg ctattgagct cgaaaaatat ttagaaagat tggtgaacct atgcttcaac
       61 aacttgtcaa tggtttgatt cttggttctg tttatgccct tttggccctt ggttatacca
      121 tggtgtacgg aattatcaaa ctcattaact tcgcccatgg tgatttgtat atgatgggtg
      181 cttttatggg gtacttccta ttaaataatt taaattttat tgcagatggt ggaacgcgtt
      241 tctttgttgc tctcattcta gcgatggtag ggacggctat tctcggtgta gttatagagt
      301 ttttagccta tcgtcctttg cgtaattcaa cgcgtatagc agctttgatt acggctatcg
      361 gtgtatcctt cttcttagaa tacactatga ttaaattctt tacagcagac gtaaaagcct
      421 ttccacaagc aatcgaagct gtgactttta accttggtcc agtttctgtt acgaatatcc
      481 agttgattat tcttggtgta tcgcttgcgt tgatggttgc acttcaatta attgtcaaac
      541 gtacaaaaat ggggaaagcc atgcgtgcgg tctctgtaga tagcgatgca gctcagctta
      601 tggggatcaa tgtaaaccgt acaatcagct ttacttttgc tcttgggtcg gctcttgcgg
      661 gtgctgcggg tgttctccta ggtctctact acaaccagat tgagcctttg atggggatga
      721 ccccaggttt gaaagccttt gtagcggcag tattgggcgg tatcggtatt attccaggtg
      781 ctgcgcttgg tggcttcgtt atcggtatca tcgaaacatt cacctatgtg attgaattcg
      841 atactttccg cgatgcgatc gtatatactg ttttgatcat catcctcttg gtgcgtccaa
      901 gtggtattct tggtaaaaat gtgaaagaga aggtgtaacc atgaagcaaa atttaaaagt
      961 taatgcgatt tggttggcta tcttattggc tggttttgga cttattcaag gtcttgtatc
     1021 agcagggatt ctgaattttt actacatcca gattgttcag caaatcggta ttcaaattat
     1081 cttggcagtt ggtttaaact tgattgttgg tttctcaggt caattttcac ttggtcatgc
     1141 tggttttatg gctattggtg cttatgcagt aggtgtcctt gggaaaatga tgccaactta
     1201 tggtggattt gctatcgcct tgattgttgg gatgttaatc tcaggtgcag tagctctttt
     1261 agtcggtatt ccaacccttc gtttgaaagg tgactacctt gcaattgcga ctctcggtgt
     1321 tgcagagatt attcgtattt taattatcaa tggtggtagt gtaacgaatg gtgcagcagg
     1381 tattatgtct attccactct ttacaagctg gcctttagtt tacatttttg taatcatcac
     1441 taccttggtg acagttaact ttttgcgcag tccgatggga cgtgctacaa tttctgttcg
     1501 tgaggatgaa attgcggcag aatctgtagg tgtcaatcca acatttgtta aagtttcagc
     1561 ctttgtattt ggtgctatga cggcagcaat tgcaggtggt ctccatgcag gatatgttgg
     1621 gacaattgtt ccgaaagact ttgcctttat gacctccgtt aacgttttga ttatcattgt
     1681 attgggtggt ttgggctcta tcacaggtac ctttgtagca gccattgtac ttggtatttt
     1741 gaacgtattc ctcaagagtt actctgatat ttcaatgatt atctattcat tagctcttat
     1801 tctcttaatg attttccgtc ctggaggtct tttgggaact aaggaattca gtgtagcggc
     1861 tctactcaag aagaaggagg ttaagtaatg gcattacttg aagtaaaaga tttaaccaag
     1921 aacttcggtg gcctgactgc cgttggtgac gtgaccatgg aacttcatga aggggaattg
     1981 gttggtctta tcggtccaaa cggtgctggt aaaacgactc ttttcaacct cttaacaggt
     2041 gtttatgagc caagcgaggg gacaatttct cttgcaggga ctattttgaa cggtaaagca
     2101 ccatcaaaaa ttgcttcgct tggtcttggt cgtactttcc agaacattcg tttgttcaaa
     2161 aatatgaccg ttttggaaaa tgttttgatt ggtcttggca accatggtaa atcagaagta
     2221 tttgcaagtt tcttccgtct tccagcattc tataagaatg aagaagcgtt gaaaacaaaa
     2281 gcgattgaac ttttgaaaat cttcgatttg gatggagatg cagatacatt agctaagaac
     2341 cttccttacg gtcaacaacg tcgtttggaa atcgttcgtg cccttgcgac tgaaccaaaa
     2401 attcttttct tggatgagcc agcggctggt atgaacccac aagaaacggc agaattgacc
     2461 caacttatcc gtaaaatcaa agaagaattt ggcattacta tcatcttgat tgaacacgat
     2521 atgagcttgg ttatggaagt aacggagcgg atatacgtat tggagtacgg tcgtttgatt
     2581 gcccatggta caccagaaga aattcgtaat aacaagcgtg taattgaagc ctaccttgga
     2641 ggtgaagctt aatggcaatg ttagaagtaa aaaatctttc cgttaactac ggtgttatcg
     2701 aagctgttaa agatgttagc tttgaggtta atgaaggtga agttgtaaca cttatcggcg
     2761 ctaatggtgc tggtaaaacc tctatccttc gtactatttc aggacttgtt cgtccatcag
     2821 ctggaacgat ttctttcctt ggaaatgaga ttcaaaaagt tcctgctcgt aaaatcgttg
     2881 cggacggttt gtcgcaagtt ccagaaggtc gacatgtctt tgcaggcttg actgttatgg
     2941 aaaacttgga aatgggtgcc ttccttcgca acaatcgtga agaaaaccaa gcaaacttga
     3001 aaaaaatctt tgctcgtttt ccacgtttgg aagaacgtaa aaaccaggat gcagctaccc
     3061 tttcaggtgg ggagcaacag atgttggcca tgggacgtgc cctaatgagc caaccaaagc
     3121 tcttgctcct tgatgaacca tcaatgggct tggctccgat ctttatccaa gaaatttttg
     3181 atatcatcca agatattcaa aaacaaggaa caactgtcct cttgattgag caaaatgcca
     3241 acaaagccct tgctatcgca gaccgtggct acgtcctaga aacaggaaaa gtcgtcctct
     3301 caggaacagg aaaagaactc ctcgcctcag aagaagtcaa aaaagcatat ctgggtggtt
     3361 aaaacgtggg ttaccccacg ttttaagttg gagatgagaa acgccggcag gcgtttttct
     3421 ttatatcaag gtctggggga cctttttagg ttggaaatga gaaccgctgg taagcggttt
     3481 cctttataag tgggttaccc cacgttttaa gttggagata ggaaaagaaa aagtctttag
     3541 aggctttttc tttgtccccc aaccctgacg ccttatttta ggttggaaat aggaaacgcc
     3601 gggaggcgtt tttctttata tcaaggtctg ggggaccttt ttaggtggaa atagggagtg
     3661 ttctctcatg ttattgctat ctatgcagtg gtcttcaaat atagtataat gaatgtatat
     3721 ttggaggttt gtatgattaa agttgaagag attttatcga ctctatcacc gaatcaaaag
     3781 gttaactatg ataaagtgtt tcaaaaaatg gcaagcacgt ggcaggaagc tgggcttcga
     3841 ccgaaaattc tcatgcatgt ctgttgtgcc ccatgctcga cctatacatt ggagtacttg
     3901 actcaatatg cagatgtaac catctatttt gcgaattcca atattcatcc aaaggcggaa
     3961 taccagcggc gggcagccgt gacggcccag tttgtcaaag aatttaatca gaaaactgga
     4021 cagtcggtag gctatctaga agctgactat agccctcagg aatttttcaa gaaaactcag
     4081 catcttgcca atgagccaga gggaggagat cggtgtaagg tttgctatga ttatcggttg
     4141 gataagactg cggagaaggc agttgagctt ggctttgact attttggttc ggctttgaca
     4201 atcagtccac ataagaattc gcagaccatc aatgctgctg ggattgaggt gcagaaatta
     4261 tacggtaccc agtatcttcc gagtgacttt aagaaaaatg gaggctatca gcgttcagtt
     4321 caaatgtgtg aagagtatga catttatcgc cagtgctatt gtggctgtgt ctatggggcc
     4381 aatgcccaag gtgtcaatct cgtacaggta aaaaaagaag ccttggaatt tttaaaaacg
     4441 cacagagatt attcccacat tcctttcaag gtgttggata agaattgagt ttttgagttt
     4501 agtttttact taaggagcgt tttttcttgc tttctctatg aaattggtat atactaattc
     4561 cggttaatat attgtactac taggagaaaa gcaaaatgaa attgagaaaa gtatcgttta
     4621 ttttagttac ctttgtcatc ggtttactat cgttatcaag tgtaaaggcg gagacttcga
     4681 tgtttgtacc agtaggtcaa cagaatgttc cgggggtaga agtagctcct tttagaactg
     4741 gttcagactc tatccatgtc catgttggtt catttggtta tgttgctaca tatataaatg
     4801 gagaacgatt gaaggtcgag cctgtaaagc atgaactcaa gtgtccgagt tatactacag
     4861 agaattgcca aactaaagag tggtatacaa gtggggaacg tgcggacggc agtggagatt
     4921 atgttgtaaa attgaataag aaactcgaag aaggtgatgt tgttacatta aaatttgcag
     4981 acgatgggaa tttgtatttt ggtcaattag tatataaaag tgaaaagaag cgagttgaac
     5041 agactcaaaa ggaacaagag gatgagtacg cagatgcctt gttcaaacgt tctattgagg
     5101 aagaagaaaa taagacttgg agagatcgga taaaggatac ttttcaagat gcttggtgga
     5161 atttcaaagg gtggtggaat agctaacaaa ggaactgcta tttggaaaat aggtcatttg
     5221 gagtgattat caattaattt tttctacctt cctgatataa atttcaatga aagagtccag
     5281 actttatgtc taggctcttt ttgtggtaca atgagaaaag aattttgagg tttttatgac
     5341 agtaattgaa cagattgaaa aattaaaaaa agagaaatta gagcttctga ccgttttatg
     5401 cggtgaagac atcggacaat accaaatagc gaaagactta cttctgcgga agcttgattt
     5461 tgatgcggca gatttaggtt ttgcctattt tgatatgtcg gaggctgatt atcatcaggt
     5521 agacttggac ctagtttctc ttcccttctt ttcagatgaa aaagtagtga ttcttgatta
     5581 ttttgcagat ttaacgacag ataaaaaacg gcatttgaca gatgatgaac tcaaacagtt
     5641 tgaggtctat ttggaaaatc ctgtcgaaac aacatgtttg gtcattctag caccgggtaa
     5701 gttggacagt aagcgtcgtc tggtcaaact cctcaaacga gatggactag tattagaagc
     5761 caatcctttg aaggagatgg atttgaaaaa tcatttccaa aaggagataa tgcgtcttgg
     5821 attgcagatg ggaggagagg tttttcaata tcttttagaa aagtccaatt ttgattttgc
     5881 agaaatcagt aagaacctag tctttcttca atcttataag ggaaaagaga cgattgaaat
     5941 ttcagatatt gatgcggcta tccccaaaac actacaagat aatatctttg atttaacaca
     6001 gttaattcta caatccaagg tagacgaggc tcgcaactta gttcgtgact tgcggttgca
     6061 aggggaggaa gaggttaagt tgattgccat catgttaacc caatttcgaa cctatctaca
     6121 ggtgcaaata ctactagagc aaggccgagg ggaacagcag atagtagctg agttatcaac
     6181 tattatgggg cgtaaggtga atccctacca agtaaagtat gctttgcgtg attcacgcca
     6241 tctgtccatt ggatttttga aaaaagtcgt caagctccta atcgatacag attttcaaat
     6301 taaaacaggc caatttgata aggattattt atttgatttg ctattattaa aaatcgcgac
     6361 tgcatagtaa aaagagtatt cttagatcga gggtcaaccg tggtttcaga caaataaatt
     6421 gaaaaggctc tcacattgga gtagaataga actatcactt agtataggag aaaacgaaaa
     6481 tggcaattat tttaccagac cttccatacg catacgatgc cttggaacca catattgatg
     6541 cagaaacaat gacccttcac cacgataagc accatgcgac ttatgttgca aatgctaatg
     6601 cagcacttga aaaacatcct gaaattggcg aagacttggt agctcttttg tcagatgtgg
     6661 agcaaattcc agctgacatc cgccaagctt tgataaacaa cggtggtggt catttgaacc
     6721 atgctctttt ctgggaattg ctttcacctg aaaaaacaga aatttcagca gaattggcag
     6781 cagatattga tgcaaccttt ggttcatttg atgctttcaa agaagccttc actgcagcag
     6841 caacaactcg tttcggttca ggttgggctt tcttggttgt taataaagaa ggtaagttgg
     6901 aagttatctc aactgctaac caagacacac caatcatgca aggtttgaaa ccaatcttgg
     6961 cattggatgt ttgggaacat gcttattacc tcaactaccg caatgtgcgt ccaaactaca
     7021 tcaaagcctt ctttgaagta atcaactggg acaaggttaa cgaactttat aaagcagcta
     7081 aataagaata gttctcttcc tttgggaaga gattttttag ttgccaggat tatttttgag
     7141 agaattgtgt aagggaagaa ggggaatttt cgtattatag ataaaaatga caagatattt
     7201 tcaga
//
LOCUS       DASFSW010000098         7120 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.098, whole genome shotgun sequence.
ACCESSION   DASFSW010000098 DASFSW010000000
VERSION     DASFSW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.098"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(317..955)
                     /locus_tag="U1099_001570"
     CDS             complement(317..955)
                     /locus_tag="U1099_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HEM4867883.1"
                     /translation="MRWGIVVEQLTVLYQNLKRRYGEFHWWNDENPIKDLVSTILIQQ
                     TTEANAKRALEQLEGRLTIHSLLEMPVEDLQECIRPAGFFKQKSLYIRSVVEWANQFD
                     GDFSRLDRVETAVLRKELLSLKGVGNETADVILLYLCRRSIFVADQYALRLFNRLGLS
                     QSQDYLSLRQEFTEQIKDWSVKDAQELHALIDEHGKQFRLTKGQLDESWLMI"
     gene            complement(1003..1923)
                     /locus_tag="U1099_001571"
                     /pseudo
     CDS             complement(1003..1923)
                     /locus_tag="U1099_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922588.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            complement(2025..2651)
                     /locus_tag="U1099_001572"
     CDS             complement(2025..2651)
                     /locus_tag="U1099_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEM4867884.1"
                     /translation="MKIAIIAANGQAGQTIAKEAVERGHQVTAIVRSENKSVAQEVIQ
                     KDAFALTKEDLAGFDVVVNAFGAWTPETLPDHARLAEHLATILAGSPTRLLVVGGAGS
                     LYLDESQTAMLKDTPDFPAEYLPIAEAMGTGLDLYRQATAVNWTYISPAADFDAEGQK
                     SGAYTLAGEVFQVNAQGESYISYADYAVALVDIAEKGGYQQERISVLA"
     gene            complement(2890..3309)
                     /locus_tag="U1099_001573"
     CDS             complement(2890..3309)
                     /locus_tag="U1099_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEM4867885.1"
                     /translation="MQISSRFTTASHILVLLALEGEKEKQTSTSIAGSVGVNPVIIRN
                     ILSQLKEAGLVEVARGVGGARLAQGPDQITLLHVYQAVELFGEKGQLFAFHEQPNPSC
                     QVGRNIHPLLDSRLENAQSALENELAKTRLADLLAEL"
     gene            complement(3352..4419)
                     /gene="dinB"
                     /locus_tag="U1099_001574"
     CDS             complement(3352..4419)
                     /gene="dinB"
                     /locus_tag="U1099_001574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEM4867886.1"
                     /translation="MLIFPLINDLSRKIIHVDMDAFFAAIEVRDNPALKGKPVIIGAD
                     PRLSGGRGVVSTCNYEARAFGIHSAMSSKEAYECCPQAIFISGNYEKYQEVGRQVREI
                     FHRYTDLVEPMSIDEAYLDVTENKLGISSAVKIAKLIQYDIWNELHLTASAGVSYNKF
                     LAKIASDMEKPYGLTLILPEDAVGILASLPVEKFHGVGKKTVERLHEMGVYTGQDLLD
                     VPEMVLIDRFGRFGFDLYRKARGISNSPVKVDRVRKSIGKERTYRKLLYREEDVLKEL
                     ISLCQRVAASLKRNGKKGRTIVLKVRYGDFSTLTKRHSLEVYTDQTETIEKEVRQLIE
                     EIGKIEKGIRLLGVTVTNFQT"
     gene            4734..7079
                     /gene="pflB"
                     /locus_tag="U1099_001575"
     CDS             4734..7079
                     /gene="pflB"
                     /locus_tag="U1099_001575"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEM4867887.1"
                     /translation="MSTKVKTKNVAEDIFAQAWEGFKGTDWQDKASVTRFVQANYTPY
                     DGDESFLAGPTERSLHIKKIIEETKAGYEDTRFPMDVDRATSIADIPAGFIDKENELI
                     FGIQNDELFKLNFMPRGGIRMAETTLIENGYTPDPLLHEIYTKHATTVNDGIFRAYTA
                     DIRRSRHSHHVSGLPDAYSRGRIIGMYARLALYGADYLMEEKVADWNAITEIDEESIR
                     LREEINMQYQALQQVVRLGDHYGVDVRKPAMNTKEAIQWTNIAFMAVCRVINGAATSL
                     GRVPIVLDIYAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAFDEIYSGDPTFL
                     TTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYSFRRYCMAMSH
                     KHSSIQYEGVTTMAKDGYGEMSCISCCVSPLDPESEDQRHNIQYFGARVNVLKALLSS
                     WNNGYDDVHKDYKVFDGVEPNTSEVFDYDQVVKNFEIALDWLTDTYVDAMNIIHYMTD
                     KYNYEAVQMAFLPTHLRANMGFGICGFANTVDSLSAIKYAQVKPIRDEDGFIYDYEVT
                     GDFPRYGEDDDRVDDIAKWLMEAFFTRLNKHKLYKNAEATVSILTITSNVAYSKQTGN
                     SPVHRGVFLNEDGSVNTTKVEFFPPGANPTSKARGGWLQNLNTLSKLNFKHANDGISL
                     TTQVSPKALGKTFDEQVNNLVTILDGYFEQGGQHVNLNVMDLNDVYEKIMAGEDVIVR
                     ISGYCVNTKYLTKEQKTELTQRVFHEVLSMDDHAAEVSGQA"
BASE COUNT         2100 a         1676 c         1309 g         2035 t
ORIGIN      
        1 taatcatttt atcttggaaa ataactccaa ttaatataaa aaaagagcaa ccaactgaac
       61 cgctggtttt actcttttat agtcgtttcg tttgctttta gtacaaggca acgatatgca
      121 ggctacttca actgtccagt ggagtgttta aactcggaaa taaggaaatg agacttcctc
      181 gcccttcagt aataaaaaat acactaattg atactcaatg aaaatcaaaa tcaagctagg
      241 cgacgcaaat gcagatagaa ctgaggttca tcaaatcatg tcaacaacgt ctgattttga
      301 ttttcgaaga gtatgactat atcatcaacc atgattcatc aagctgcccc tttgttaacc
      361 tgaactgctt gccatgttca tcaatcagtg catgaagctc ctgagcgtct tttaccgacc
      421 aatcttttat ctgctctgta aattcctgtc tcaaactcaa ataatcctga gactggctaa
      481 gtcctaaacg attaaatagc cgaagagcat attgatccgc cacaaaaata cttcttcgac
      541 acaaatagag caaaataaca tccgcagttt cattgccaac ccccttcaag ctcagcagct
      601 cttttcgaag tactgctgtc tcaacacgat ctaggcgaga aaaatctcca tcgaactgat
      661 tggcccactc aaccactgac cgaatgtaga gtgatttttg cttgaagaat cctgctgggc
      721 gaatgcattc ttgtaaatcc tccacaggca tctctaaaag cgaatgaatt gtcaaccgtc
      781 cctccaactg ttctaaagca cgcttggcat ttgcttccgt tgtttgctga ataagaatcg
      841 tagataccaa atcctttata ggattttcat cattccacca gtgaaattca ccatatcgcc
      901 gttttaaatt ttggtaaaga accgtcaact gttccactac tatcccccat ctcaccttgt
      961 ttctccttgc cattatagca cgatgagtaa agggatacaa tactacagtt cctctagtac
     1021 tattgtatct acaatcgctt caaactgctc tgcttcaacc tctggaaaat aattcaaaca
     1081 attggctgtt gcaaaggctg ccgcttcttt caaaaagatt tctggggctt gaccttgact
     1141 gattcctttg actagcaagc ccaggtagaa atcccctgcg gcaataggat tacgcgtttc
     1201 tttctttgga gactaaatac ggaagaagcg ctgaccaatc ttagctagac tccccttgtc
     1261 tcccatggta atgatgatat tctcatgagg agtcacttct ttcaggatac ctaaaatagc
     1321 ttcaggactt tcttcatcca gctctggata aatatctttc agctcttcat tattgatttt
     1381 gataagggcc ggtctagctt gataagccgc acgtaaagct ggtccactgg tgtccactat
     1441 ggtcagaact tctgggtatt tttcaataaa ccgctgaata taatcatccg acaccccctt
     1501 catcagacta cctgaaaaga ctaggatgtc gccagcttgc aggctatttt caatctgctg
     1561 agtgaattgg ttgagcaaat cctctgttag ttcaaaacct ttttcataaa atagtactac
     1621 atcgcctgta ctggtttcaa catagatatt acatgtccga gtattctggc tattttcaac
     1681 cacgcgcagg gcatttccac catcagcatt aagctcctga atatagcgac caatctgccc
     1741 acctaaaatg gtatggatag tgtagtctgt cactccattt tctcgaagtg cataggctac
     1801 gttgaaactt tttccaccag gataatccct cacttccgtc ggtctcaggg gaatattctt
     1861 ttcaatcttt tccacaagaa gcgtgcggtc tagggctgga ttgggacaaa tcaagtgaat
     1921 catggggttc tccttttctt tttatttata ttattatact atatgcaagt aaaaaataaa
     1981 aagataattc ctcgatggag ctatctttta gaccatttaa ttttttatgc aagtacggaa
     2041 attcgttcct gctggtagcc acctttttcc gcaatgtcaa ccaaagccac agcatagtcg
     2101 gcataactaa tatagctttc accttgggca ttgacttgga aaacctcgcc agccaaagtg
     2161 taggcacctg atttttgacc ttctgcatca aaatctgctg ctgggctgat ataggtccag
     2221 ttgaccgctg tcgcctgacg atagaggtct agaccagtac ccatagcctc agcgattggc
     2281 aggtattccg cagggaaatc tggtgtatct ttcagcatag ctgtctggct ctcgtctaaa
     2341 taaagactgc ctgcaccacc aactactaaa agacgagttg gactaccagc aagtatggtc
     2401 gctagatgct cagcaagacg agcgtgatcc ggcaaggttt ctggtgtcca agcaccaaaa
     2461 gcattaacta ctacatcaaa gcctgccaaa tcttctttgg taagagcaaa agcatccttc
     2521 tggattactt cttgggctac gctcttgttt tcagaacgga caatagccgt cacctgatga
     2581 ccacgttcta ctgcttcctt tgcaatagtt tgacctgctt gaccattcgc tgcaataata
     2641 gcaattttca tattgatacc tcacttgttt cgttgtaaca acttcgttta caacgtaagt
     2701 ataaattatt ttggttgtaa cgtcaattgt tacagcttga aaattaaaaa aatcatcaca
     2761 aggatgattc actaactagt cttttagtgc tagggcaaca ctctacagac tacttcaaca
     2821 gtccagtgga gtgtttgaag taggaaatag ggaaacaaag tttctcctta cgttgaccta
     2881 ataaaacgac tatagttccg ccaagagatc agcaagcctt gtcttagcaa gttcattttc
     2941 caaagccgac tgagcatttt ccaaacggct atctagcaga ggatgaatat tgcgacctac
     3001 ttggcaggaa ggattcggtt gctcatgaaa agcgaataat tgaccttttt ctccaaataa
     3061 ctccacagcc tgatagacat gcaaaagtgt aatctggtct ggaccctgtg ccaagcgagc
     3121 acccccaaca ccacgcgcta cttctaccag accagcctcc ttgagttggg acaaaatatt
     3181 tcggataatg acaggattaa ccccgacact gcctgcaatg ctagtactgg tctgtttttc
     3241 tttttctcct tccagagcca agagaaccaa gatatggctg gcagtggtaa atctacttga
     3301 aatttgcatc tcttctcctt tttttcagtc tatcatagac tgaaacagaa ctcaagtttg
     3361 aaaattagtc acggtcacac ccagcaaacg aatccctttt tcaatcttcc caatttcttc
     3421 aattagttga cgaacctctt tctcaatcgt ttccgtttgg tccgtataca cctctagact
     3481 atgacgtttc gtcaaggtag aaaaatcacc ataacgtacc tttaaaacaa ttgttcgccc
     3541 cttttttcca tttcgcttca aactggctgc gacccgttgg cagagactga ttagctcttt
     3601 caggacatcc tcctcccgat aaagaagttt gcgataggtt ctttcctttc caattgactt
     3661 acgaacacgg tccactttaa cgggcgaatt tgaaatcccc ctcgctttcc gataaaggtc
     3721 gaagccaaaa cggccaaatc tatctatcaa aaccatttct ggtacatcta gtaaatcttg
     3781 ccccgtgtag acccccatct catgcaggcg ttccaccgtc ttcttcccca caccgtgaaa
     3841 tttttcaaca ggcagggagg cgagaatacc aacagcatct tcaggtagaa tcaaggtcaa
     3901 accgtacggt ttttccatat cagatgcaat ttttgccaaa aatttattat aggaaacgcc
     3961 tgcggaagct gtcagatgca attcattcca aatatcgtat tggataagct tggcaatctt
     4021 aaccgcagag ctgatgccca acttattttc tgttacatcc aagtaagcct catcaataga
     4081 catgggctcc accaaatcag tatagcgatg aaaaatttct cgaacctgtc gcccaacttc
     4141 ctgatatttt tcataattgc cagaaatgaa aatcgcctga ggacaacact cataggcctc
     4201 ttttgaggac atagccgagt gaatcccaaa ggcacgcgcc tcataattac aggtagatac
     4261 cactcctcta ccgccagata gtcttgggtc agccccaatg ataacaggtt tgcccttcaa
     4321 agcgggatta tccctgacct caatggcagc aaaaaaagca tccatatcta catgaataat
     4381 ttttctagat aaatcattga ttaagggaaa aattaacatg actgcctctt cctgtttaca
     4441 agtttgactc gctatattat accgcatttc ttaggaagtt tcagccttta gattcgttga
     4501 accagccatc ttatcaatac aataaacgac agcttatcac cttattacaa atacaagcag
     4561 aaatttattt tgaaaatacc aactctatct ttcaattttt tcatattcct tgaaaggttt
     4621 ttcatttatt ttcaagtatt tttgtcttgt agaaagtgtt ttcaaatggt ataataatga
     4681 catgttggta tcgatagcaa gtcgataccc aaaagcataa aaggagaacc ctcatgtcaa
     4741 caaaagttaa aacaaaaaat gttgctgaag acattttcgc ccaagcctgg gaaggcttta
     4801 aaggtacaga ttggcaagat aaggcaagtg taacacgctt cgttcaagcc aactacactc
     4861 catacgatgg agatgaaagc ttcctcgcag gcccaactga gcgttcactt catatcaaaa
     4921 aaatcatcga agaaacaaaa gctggctacg aagacactcg cttcccaatg gacgtagatc
     4981 gtgctacttc tatcgctgat attccagcag gtttcatcga taaagagaac gaactcatct
     5041 tcggtatcca aaacgatgag ctcttcaaac tcaatttcat gccacgtggt ggtattcgta
     5101 tggctgaaac aactcttatc gaaaacggct atactccaga cccactcctt catgaaatct
     5161 atacaaaaca cgctacaact gtaaatgacg gtatcttccg tgcctacaca gctgacatcc
     5221 gccgttctcg ccactcacac cacgtttctg gtcttccaga tgcatactca cgtggtcgta
     5281 tcatcggtat gtacgctcgt ttggcacttt acggtgcaga ctacttgatg gaagaaaaag
     5341 tagctgactg gaatgcgatt actgaaatcg acgaagaatc aatccgtctt cgcgaagaaa
     5401 tcaacatgca gtaccaagca ttgcaacaag ttgtacgctt aggtgatcac tatggtgttg
     5461 atgttcgcaa acctgcaatg aacactaaag aagctatcca atggacaaac attgctttca
     5521 tggctgtatg tcgtgttatc aacggtgcag caacttctct tggtcgtgtg ccgatcgttc
     5581 ttgacatcta tgcagagcgt gacttagctc gtggtacatt tactgaatct gaaatccaag
     5641 aatttgttga tgacttcgta ttgaaacttc gtacagtgaa attcgctcgt acaaaagcct
     5701 tcgacgaaat ctactctggt gacccaacat tcttgacaac atctatggca ggtatgggta
     5761 acgatggtcg tcaccgtgtt actaagatgg actaccgttt cttgaacaca cttgacaaca
     5821 tcggtaactc tccagaacca aacttgacag ttctttggtc agaccaactt ccatattcat
     5881 tccgtcgcta ctgtatggca atgagccaca aacactcttc tatccaatac gaaggtgtga
     5941 caacaatggc gaaagatggt tatggcgaaa tgtcatgtat ctcttgctgt gtatctccac
     6001 ttgatccaga aagcgaagac caacgccaca acattcagta cttcggtgct cgcgtaaacg
     6061 tccttaaagc tcttctttca agctggaaca acggttacga tgacgttcac aaagattaca
     6121 aagtattcga cggtgttgaa ccaaacacat ctgaagtatt tgactacgac caagtagtta
     6181 agaactttga aatcgccctt gactggttga ctgatacata tgttgatgcc atgaatatca
     6241 tccactacat gacagataaa tacaactacg aagcagttca aatggccttc ttgccaactc
     6301 accttcgtgc taacatgggc ttcggtatct gtggtttcgc aaacactgtt gactcattgt
     6361 cagcaatcaa atatgcacaa gttaaaccaa tccgtgacga agatggcttc atctacgact
     6421 acgaagtaac tggtgacttc ccacgctacg gtgaagacga tgaccgtgta gatgatattg
     6481 cgaaatggct catggaagca ttcttcactc gtttgaacaa acataaattg tacaagaatg
     6541 ctgaagctac agtatctatc ttgacaatca cttctaacgt tgcttactct aaacaaacag
     6601 gtaactctcc agttcaccgt ggtgtattcc tcaatgaaga tggttctgtg aacactacta
     6661 aggtagaatt cttcccacca ggtgctaacc caacttctaa agctcgtggt ggttggttgc
     6721 aaaacttgaa taccctttct aaactcaact tcaaacatgc caacgacggt atctcattga
     6781 ctactcaagt ttctccaaaa gcgcttggca aaacattcga tgagcaagta aacaacttgg
     6841 taacaatcct tgatggttac tttgaacaag gtggtcaaca cgttaacttg aacgttatgg
     6901 acttgaacga cgtttatgaa aaaatcatgg ctggtgaaga tgttatcgtt cgtatctctg
     6961 gatactgcgt aaacactaaa tacctcacta aagagcaaaa aactgaattg actcaacgtg
     7021 tcttccacga agttctttca atggacgacc acgctgctga agtttcaggt caagcttaat
     7081 caagcaaaca caaaaggact tgcaatgcag gtccttttta
//
LOCUS       DASFSW010000099         7016 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.099, whole genome shotgun sequence.
ACCESSION   DASFSW010000099 DASFSW010000000
VERSION     DASFSW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.099"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(116..454)
                     /locus_tag="U1099_001576"
     CDS             complement(116..454)
                     /locus_tag="U1099_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEM4867888.1"
                     /translation="MSTNIYDIANELERAIRNLPEYKAVEAVKVSVEGNSEAKEILES
                     YISFQKEIQSKLQAGEIPTEADQKKMLDFNKKVQGNPLLTEYFSKQQQLGTYVADLER
                     IIFKPLNELL"
     gene            complement(464..1567)
                     /locus_tag="U1099_001577"
     CDS             complement(464..1567)
                     /locus_tag="U1099_001577"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEM4867889.1"
                     /translation="MKKTILIVGLGLIGSSLALCIRKDHPDYHLIGFDPNEQSCQIAL
                     GKGMVDEVSTELEGVAKQADVILLCVPIQVSLRLIEEFSALELKDTVLITDAGSTKSA
                     IVAAAEEHLAPRQINFIGGHPMAGSHKSGASAADLHLFENAYYIMTPCQATKQDAVPR
                     LTDLLSGTGARFVQIDAAEHDRVTSQISHFPHVLASSLMHQAGEYAQEHSFTNNFAAG
                     GFRDMTRIAESEPSMWTSILLTNADSILNRIEEFQERLSEISDVLRSGDQKAIWDFFD
                     KGRQTRRAMEIHKRAGVDSFYDLFLSVPDEEDTILKVLEVLRGISIVNIRINEENRED
                     IHGILQITFKNKGNLEDAAKRLREQTTYKIHLD"
     gene            complement(1575..2741)
                     /gene="aroC"
                     /locus_tag="U1099_001578"
     CDS             complement(1575..2741)
                     /gene="aroC"
                     /locus_tag="U1099_001578"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEM4867890.1"
                     /translation="MRYLTAGESHGPRLTAIIEGVPAGLPLTAEDINEELKRRQGGYG
                     RGSRMQIETDRVEITAGVRHGKTTGAPITLNVTNKDHQKWLDIMAVEDIEEKLKSKRR
                     IKHPRPGHADLVGGMKYRFDDLRNSLERSSARETTMRVAVGAVAKRILAELDIEIANH
                     VVVFGGKEIDVPDGLSVAEIKERAAQSEVSIVNQEREEEIKAYIDQIKRDGDTIGGVI
                     ETVVGGVPAGLGSYVQWDKKLDAKLAQAVVSINAFKGVEFGVGFQAGYLKGSQVMDEI
                     LWSQEKGYTRRTNNLGGFEGGMTNGEALIIRGVMKPIPTLYKPLMSVDIDTHEPYKAT
                     VERSDPTALPAAGVVMESVVATTLATEILEKFSSDNMEELKAAVASHRDYVKNF"
     gene            complement(2745..3419)
                     /locus_tag="U1099_001579"
     CDS             complement(2745..3419)
                     /locus_tag="U1099_001579"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEM4867891.1"
                     /translation="MKIVVPIMPRNLEEVETIDVERLAEADIVEWRADYLPKDDILRV
                     APAIFEKCSGKEVVFTIRTTREGGHLDLDDQEYVNVIKEVATLYQPDYIDFEYYSYKS
                     VFEQMLEFPNLVLSYHNFEETPSNYMEIMSELTSLSPAVVKMAVMAKTEQDVLDVMNY
                     TRGFKSLNTEQIFATIAMGELGKLTRIAGVITGSCWTFASLDETSAPGQMSLSNTRKF
                     LEILEN"
     gene            complement(3416..4579)
                     /locus_tag="U1099_001580"
     CDS             complement(3416..4579)
                     /locus_tag="U1099_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEM4867892.1"
                     /translation="MKIKVNRIAEQKINRGIQLLDSADFSGVSFDEDRLAVLYSESGS
                     YLGQAYLSKQNKGIGWLFSRSNQDLTMDFFVKLFVDAKQKRQHFYQSEMTNAFRLFNQ
                     DGDSFGGMTVDLYDEYAVFSWYNTFIYSVKEMIVAAFQQVFPEVRGAYEKIRFKGLAF
                     ESAHLYGEEAPEYFTVLENGVHYQVFMNDGLMTGIFLDQHEVRGSLVDGLAAGKSLLN
                     MFSYTAAFSIAAAMGGAEETTSVDLAKRSRELSKAHFKVNSLDLDNHHFVVMDVFEYF
                     KYAKRKGLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNTGGTIIASTNAA
                     NVSVEKFKRQIEKGFDVRKHRYEATYRLPADFVINKNDESSNYLKVFTIQVDK"
     gene            4695..6869
                     /locus_tag="U1099_001581"
     CDS             4695..6869
                     /locus_tag="U1099_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM4867893.1"
                     /translation="MKKLIDYVKKSGQTRLGFVLILLIIYWLKTLWAYYVDFNLDTQG
                     FFQNFIAIMNPLPVGILLLGLSLYIKNTKVFYSVAMIIYIILSVWLFSNAVYYREFSD
                     YISISTIMATSSVSAGLGEAALKLFRLWDIVYFLDFIIFGILVYRKLLRWDKKTFHKK
                     SSFAITALSVMLFSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFLGYNANQTYNAH
                     KNRSGASATDLEPVKQYITEHYAQPNDNYFGIAKGRNVIYLHLESLQQFVIDYKLNVN
                     GVEYEVTPFLNSLYHSSSTLAFSNFFNQVKAGKTSDAETMIETSLFGLNQGSFMVQYG
                     GSNTQQAAPYILSQNGGYTSAVFHGNAASFWNRNNTYKQWGYNYFFDQSYFSKATAEN
                     SFQYGLNDKIMFADSIQYLEHLQQPFYTKFITVSNHYPYTTSLIGDEIGFPLANTDDE
                     TINGYFATANYLDASIKAFFDYLKATGLYENSVIVLYGDHYGISNSRNPDLAPLIGKD
                     SQTWSDFDNAMMQRVPYMIVVPGMTNGGIFDTYGGEIDALPTLEHLLGIKSDNYLQVG
                     QDLLSSQHSQIVALRTAGSFITPKYTSYEGKIYYTETGLEITNPDETTQKEIDAIKEA
                     TAAQLATSDAIQTGDLIRFFDNGLPALDPSQYNYIDSLAAEIALENKLGKQSTSLYST
                     NGNKSTIELFKAPSYLELHGSTTATSTPNTGTSSTSTSSNSR"
BASE COUNT         2088 a         1619 c         1192 g         2117 t
ORIGIN      
        1 tactccccaa gtaccgtttc tgaatctgta cttggagata agaactctac gtaagcagcg
       61 tatactaggc gtcaatccat gacctaacac cctgctccta tcaaatataa atatattaca
      121 acaattcgtt gagaggctta aagatgatac gttccaaatc agctacataa gttcccaact
      181 gttgttgctt gctgaaatat tccgtcaaaa gtggatttcc ctgaactttc ttgttgaaat
      241 ccaacatttt cttttggtca gcttcagtag gaatttcacc agcctgcaat ttagattgga
      301 tttctttttg gaaagaaata taactttcca atatttcttt ggcttctgaa ttcccctcaa
      361 ctgagacctt aacggcttcc accgccttat attcaggcag gttacggatt gcacgttcca
      421 attcatttgc gatatcatag atatttgttg acatattttt ctcctaatct aaatggattt
      481 tgtaagtcgt ctgctctctt aaacgtttcg cagcgtcttc caaatttccc ttgtttttaa
      541 acgtgatttg taaaataccg tgtatatctt cacggttctc ttcattgata cgaatattga
      601 caatggaaat accccgtaag acttccaaaa ccttcaaaat cgtatcttcc tcatcaggaa
      661 cactaaggaa taaatcgtag aaagaatcaa cacctgcacg cttgtgaatt tccatggctc
      721 tacgagtttg acgtccctta tcaaaaaagt cccaaattgc tttttgatcg ccagatctga
      781 gcacatctga aatttctgaa agacgttctt gaaattcctc gattcgattt agaatagaat
      841 ctgcattggt tagcaaaatg ctagtccaca tgctcggctc actttccgca atccgtgtca
      901 tatctctaaa tcctccagct gcaaaattat tcgtaaagga atgttcttgg gcatactcac
      961 cagcttgatg catcaaacta gaagccaata cgtgtggaaa atgtgaaatt tgactggtca
     1021 cacggtcatg ttctgctgca tcaatctgaa caaaacgtgc ccccgttcca gataacaagt
     1081 ctgtcaatcg gggaaccgca tcctgcttgg tagcctgaca cggtgtcata atgtagtaag
     1141 cattctcaaa tagatggaga tctgctgcac tggcaccaga tttatgacta cctgccatag
     1201 ggtgtccacc gataaaatta atttgtcttg gcgccaaatg ttcctcagca gcagccacaa
     1261 ttgcagactt cgttgaacct gcatctgtaa tcaagaccgt atccttcaat tcaagggcac
     1321 taaattcttc aatcaaacgc aaagaaactt gaattggaac acaaagaaga atcacatcag
     1381 cctgctttgc tacaccctcc aattccgttg atacctcatc aaccatcccc ttgcccaagg
     1441 caatttggca tgattgttcg ttaggatcaa agccaatgag gtgatagtca ggatggtctt
     1501 tgcgaataca gagcgcaagt gagcttccaa tcaaccctag acctacaatt aaaatcgttt
     1561 ttttcattaa ttacctagaa atttttgaca tagtcgcgat gactggctac tgcagctttc
     1621 aattcctcca tattatcgga agagaacttc tctaggattt ctgtcgctag agttgtagct
     1681 acgactgatt ccatgactac accagctgct gggagagccg ttggatctga acgctccaca
     1741 gtagctttat aaggctcatg agtgtcaatg tcaaccgaca tcaatggctt atagagggtc
     1801 ggaattggtt tcataacacc acgaataatc agagcctcgc cattggtcat accaccttcg
     1861 aaaccaccta gattattggt tcggcgtgta tatccttttt cttgggacca gagaatttcg
     1921 tccataactt gactcccttt gagatagcca gcttggaagc ctactccaaa ttctactccc
     1981 ttaaaagcat tgattgaaac aacagcctgc gctaattttg catctaactt tttatcccat
     2041 tgcacatatg accccaagcc agctggaact cctccaacaa cagtctcaat cactccgcca
     2101 atagtatctc catctcgctt gatttgatca atataggctt tgatttcttc ttcacgctct
     2161 tggttgacaa tggatacttc tgactgggct gcacgctctt tgatttctgc tacgcttaaa
     2221 ccatctggga catcaatttc cttccctccg aaaacgacta catggttagc aatttcaata
     2281 tccaactcag ccaaaatccg tttggcaacc gcaccaacag ccacacgcat agttgtctca
     2341 cgcgcgctag aacgctccaa ggaattacgc aaatcatcaa agcgatattt cataccacca
     2401 actaggtcag catgtcctgg tcgtggatgt ttgatacgac gcttactttt gagtttctct
     2461 tcgatgtcct caactgccat aatatccaac catttttgat ggtctttgtt ggtcacattc
     2521 aatgtaatcg gggcaccggt tgtcttaccg tgacgaacac cagctgtaat ctctacgcga
     2581 tctgtttcaa tctgcatacg actaccacga ccgtatccac cttggcgccg ttttaactct
     2641 tcattaatat cctcggctgt taaaggaaga cccgctggaa ctccctcaat aatggctgtt
     2701 agacgtggtc catgagactc accagctgtt aaataacgca taacttaatt ctccaaaatt
     2761 tccagaaact tacgagtatt gcttaaagac atttgtcctg gtgcagatgt ttcatctaag
     2821 ctggcaaaag tccagcagga gcctgtaatc acacctgcaa tacgtgttaa cttccctagt
     2881 tcacccatgg cgattgtcgc aaatatctgc tcagtgttca gtgatttaaa accacgagta
     2941 taattcataa catctaggac atcctgctct gtcttagcca ttacagccat tttaacaaca
     3001 gctggtgata ggctagtcag ttcagacatg atttccatat aattagacgg tgtttcttca
     3061 aaattatgat aactcaacac aagattagga aactccaaca tctgctcaaa aaccgactta
     3121 taagagtagt attcaaaatc aatataatct ggctgataaa gcgttgctac ttctttgatt
     3181 acattgacat attcttgatc gtccaaatcc aagtgaccgc cttcacgtgt tgtccgaatg
     3241 gtgaaaacta cttccttacc actacatttt tcaaatatag caggtgctac tcttaaaata
     3301 tcatctttcg gaagatagtc tgctcgccat tcaacaatat ctgcctctgc tagacgttct
     3361 acatcaatgg tctctacctc ttccagattt ctaggcataa taggaactac tattttcatt
     3421 tatctacctg tatcgtaaag acctttaggt aattactact ttcatcattt ttattgatta
     3481 caaaatcggc tggcaagcga taagttgcct catagcgatg ttttctaaca tcaaaccctt
     3541 tttcaatctg ccgcttaaat ttttcaacag aaacatttgc ggcgttggtc gaagcgataa
     3601 tagttccacc tgtatttagt atttccaaag attgagaaat caacttgtga taatctttgg
     3661 ctactgaaaa ggtttgtttc ttatttcgtg caaagcttgg cggatccaag acaatcacat
     3721 cataagttaa gcctttacgt ttggcatatt tgaaatattc aaatacatcc attactacaa
     3781 agtggtgatt atctaagtct aaactattaa ccttaaagtg agctttagaa agttctcgcg
     3841 agcgtttggc taagtcaaca gaagtcgttt cctctgctcc gcccatggca gcagcaatag
     3901 aaaatgctgc cgtatacgag aacatgttca aaagtgactt gcctgccgcc aggccatcca
     3961 ccaaggaacc acgtacctca tgctgatcca aaaagattcc cgtcatcagt ccatcattca
     4021 taaagacctg atagtgaact ccattttcca acacagtgaa atactcaggg gcttcttctc
     4081 cataaagatg agcagactca aaggccaaac ctttaaaacg aatcttctca taagctcctc
     4141 ttacttctgg aaatacctgt tgaaatgcag caacaatcat ctctttgaca gaataaataa
     4201 aggtattata ccatgaaaaa actgcgtatt catcatacaa gtcaaccgtc attccaccaa
     4261 aactatctcc atcttgatta aataaacgaa aagcgttcgt catctcagat tgataaaaat
     4321 gttgacgttt ctgtttagca tccacgaata atttaacaaa aaaatccatg gtcaagtctt
     4381 gattgcttct actaaacagc cagcctatcc ccttattctg tttggacaaa taagcctgac
     4441 ccaaatagct tccagactct gaatacaaaa cagccagcct gtcttcatca aatgaaacac
     4501 ctgaaaaatc agcgctatcc aagagttgaa tccctctgtt tattttctgt tctgcaatac
     4561 ggtttacctt tattttcata gaattattat aacatgtttc ttcatatatg ccaaaaaaac
     4621 tctaaacttt gaggcttatt ttagaattat ggtataatga actaaacttt tagaaaggac
     4681 catgaggttt tattgtgaaa aaactaattg actatgtcaa gaagagcgga cagactagac
     4741 ttggtttcgt cttgattttg ctaatcattt actggctcaa aacactctgg gcctactatg
     4801 ttgatttcaa cttagataca caagggtttt tccaaaattt tattgctatt atgaatcctc
     4861 taccagtcgg gattttatta ctcggtctct cgctttatat aaagaataca aaagtcttct
     4921 atagcgtagc gatgatcatt tatattattc tttccgtctg gctcttttca aatgcagtct
     4981 attacagaga attttccgat tatatttcca tctctactat aatggctacc tctagtgtat
     5041 ctgctggatt aggagaagca gctcttaagt tatttaggct atgggatatt gtctattttc
     5101 ttgattttat catttttgga atcctagtct atcgtaaact actccggtgg gataaaaaga
     5161 catttcataa aaaatctagc tttgctatta cagcactatc agtcatgctt ttctccatca
     5221 acctcttttt agcagaaatt gatagaccag agctacttac gagaggattt tctaatactt
     5281 atatcgtcag agcccttggt ttacctgctt tccttggtta caatgctaat caaacctaca
     5341 atgcccacaa gaatcgttct ggagcatcag ctacagattt ggagcctgtg aaacaatata
     5401 ttacagaaca ttatgctcag ccgaatgaca actacttcgg tattgccaaa ggacgaaatg
     5461 ttatctatct ccacctagaa agcttacaac aatttgttat tgactataag ttgaacgtca
     5521 acggtgtcga atacgaagtc acacctttcc tcaattctct gtatcattct agctctactc
     5581 tcgccttttc taatttcttc aaccaagtga aggcaggtaa aacctctgat gccgaaacca
     5641 tgattgaaac ctcacttttt ggactcaatc aaggctcttt catggtccaa tatggcggaa
     5701 gcaataccca gcaagctgca ccatatattc tctctcaaaa tggtggctat acatctgctg
     5761 tattccacgg aaatgcagcc agtttctgga ataggaacaa cacctataaa caatggggtt
     5821 acaattactt ctttgatcaa tcctatttct ctaaagccac tgctgaaaat tcattccaat
     5881 acggtctgaa tgataaaatc atgttcgccg actctatcca atatctggaa catttacagc
     5941 aacccttcta taccaaattt atcacagttt ccaaccacta cccctacaca accagcttga
     6001 ttggggacga gattggcttt ccattagcca atacagatga tgaaaccatt aatggctatt
     6061 ttgctactgc aaactatctc gatgcgtcca taaaggcatt tttcgattac ttaaaagcga
     6121 ctggcttgta cgaaaactcc gttatcgtcc tctatggtga tcactatgga atttctaatt
     6181 cacgcaatcc tgacttggca ccgttgattg gaaaagattc tcagacttgg tcagattttg
     6241 acaatgccat gatgcaacgt gttccatata tgattgttgt acctggaatg accaacggcg
     6301 ggatttttga tacctacggt ggtgagattg atgcacttcc tactctcgaa catcttttag
     6361 gaatcaaatc cgataactat cttcaagttg gtcaagacct actttccagc caacacagcc
     6421 aaattgtggc tcttcgaacc gctggcagtt tcatcacacc aaaatacact agctacgaag
     6481 gtaaaatcta ctatacagaa actggtcttg aaattacaaa tccagatgag acaactcaga
     6541 aagaaattga tgcaattaaa gaagctaccg cagcacaact agcgactagc gatgccattc
     6601 aaactggtga tcttatccga ttcttcgaca atggattgcc agccttggat ccaagccaat
     6661 ataactacat tgactcattg gctgccgaga ttgcactaga gaataaactt ggcaaacaat
     6721 caacaagttt gtatagcaca aacggcaata agtcgacaat tgaactattt aaagcaccaa
     6781 gctacctaga acttcacggt agtacaactg ctactagcac accaaatacc ggcactagct
     6841 caacaagtac cagttcaaat tcacggtaat acactaaaag gcttagaatc atgtcagtta
     6901 ctacattgat tctaagcctt tttatactca atgaaaatca aaaacagact agctccaaag
     6961 gtttggggaa cctttggagg ttggagatag agcgaacgta gttcgtttct acttag
//
LOCUS       DASFSW010000100         6899 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.100, whole genome shotgun sequence.
ACCESSION   DASFSW010000100 DASFSW010000000
VERSION     DASFSW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.100"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(65..2332)
                     /gene="glgP"
                     /locus_tag="U1099_001582"
     CDS             complement(65..2332)
                     /gene="glgP"
                     /locus_tag="U1099_001582"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEM4867894.1"
                     /translation="MINLQNFVQSMYAKRIEDCTDQELYYALLAFTKQQSEAKYTNDQ
                     KKKVYYISAEFLIGKLLSNNLINLGLYDEVKSQLVAAGKDLIAIEDMELEPSLGNGGL
                     GRLAACFLDSIASLGLNGDGVGLNYHFGLFRQLFEYHQQSAVPNEWITPRSWLTESPI
                     SYQVPFANFTLTSKLYDIDVPGYKMERKNRLRLFDLGSVDDNIIYDGIEFDKEDIFRN
                     LTLFLYPDDSTDEGKVLRIFQQYFMVSNAAQLLIDEAVAKGSNLHDLPDYAVVQINDT
                     HPSLVIPELIRLLELRGISFDEAVEIVKKMTAYTNHTILSEALEKWPLDFLNYVVPHL
                     VPFIVELDRRAKEVKDDPAVNIIDEYGRVHMAHMDIHYGYSINGVAALHTEILKTSEL
                     KAFYELYPEKFNNKTNGITFRRWLMHANPSLASYLDGLLGHGYHHDASELEKLLDYKN
                     DKELKSWLEKTKQHNKRKLQRHIAKTQGVHVNPESIFDVQIKRMHEYKRQQMNVLYVI
                     HKYLDIKAGNIPTRPLTVFFGGKAAPAYTTAQDIIHLILVLSQVIKNDSEVAPHLQLV
                     MIENYNVTEASFIIPAADISEQISLASKEASGTGNMKFMLNGALTIGTDDGANVEIHE
                     LVGDENIYIFGQDSQTVIEHYANRTYHPYSFYEREVIRPLIDFITSDTIRHAGGIDER
                     LWRLQDNLKHNDHFMTLLDLEDYIVTKERMLADYEDRDSWLEKVIVNIAKAGFFSSDR
                     TIQQYNEDIWHLEAK"
     gene            complement(2508..3221)
                     /gene="deoD"
                     /locus_tag="U1099_001583"
     CDS             complement(2508..3221)
                     /gene="deoD"
                     /locus_tag="U1099_001583"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM4867895.1"
                     /translation="MSIHISAKPGEIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYAHELINEYGVKKLIRVGTAGSLNEDVHVRE
                     LVLAQAAATNSRMINIDWPEYDLPQIADFDLLDKAYHIAKELNMTTHVGNVLSSDSFY
                     SPKLFSRNLELGQLGVKAVEMEAAALYYLGAKFGVQTLAIMTISDSLVNPEENTTAEE
                     RQNTFTDMMKVGLETLIAE"
     gene            complement(3254..4063)
                     /locus_tag="U1099_001584"
     CDS             complement(3254..4063)
                     /locus_tag="U1099_001584"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM4867896.1"
                     /translation="MSLLAKINETKTFLEEKGLVKPEFGLILGSGLGELAQEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPMEVVTFPVRVMKA
                     LGCEGVIVTNAAGGIGYGPGTLMAITDHINMTGQNPLIGENLEEFGPRFPDMSNAYTK
                     EYREKAHAVAEKLGIKLDDGVYLGVTGPTYETPAEILAFKTMGAHAVGMSTVPEVIVA
                     AHSGMKVLGISAITNFAAGFQSELNHEEVVEVTEQIKGNFKGLVKAILAEL"
     gene            complement(4075..4431)
                     /locus_tag="U1099_001585"
     CDS             complement(4075..4431)
                     /locus_tag="U1099_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family transcriptional regulator"
                     /protein_id="HEM4867897.1"
                     /translation="MEKLTVYYNPNCSKCKKLRILLSSQDMEVKWVNYLETPLRGAEL
                     TALLEKMGSQPSAVTRLKEEERLALSEEEIFERLVEEPATLNRPIIEREQTAFLCRPL
                     EIIKEKMPEYDWSDYL"
     gene            complement(4431..5642)
                     /locus_tag="U1099_001586"
     CDS             complement(4431..5642)
                     /locus_tag="U1099_001586"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEM4867898.1"
                     /translation="MPKFKRVHLVVMDSVGIGAAPDSDKFFNAGVADTESDTLGHISE
                     KAGLEVPNMTKIGLGNIPRDTALATVPAESHPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWDGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFSRT
                     ANRHDYAVSPFKATVLNKLADAGVPTYSVGKISDIFNGSGITNDMGHTKSNMHGVDVL
                     IDTLKLSDFEEGFSFTNLVDFDAVYGHRRNIEGYRDCLQEFDARIPEIIDNMREDDLL
                     LITADHGNDPSYAGTDHTREYVPLLAYSKAFKGSGVLPVGHFADISATVAENFGVDTA
                     MIGESFLSQLV"
     gene            complement(5971..6645)
                     /gene="rpiA"
                     /locus_tag="U1099_001587"
     CDS             complement(5971..6645)
                     /gene="rpiA"
                     /locus_tag="U1099_001587"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEM4867899.1"
                     /translation="MTNLKEQVGIKAAEFVTDGMIVGLGTGSTAYYFVQEIGRRVAEE
                     GLQITGVTTSHATAEHAASLGIPLKNIDEVEYVDLTVDGADEVDGDFNGIKGGGAALL
                     MEKVVAVNSKDCIWIVDESKVVQTLGAFKLPVEVVQYGAENLFRLFEKKGYRPSFRMR
                     NGEKHITDMQNFIIDLDLRRIEDTYALAEELDRTVGVVEHGLFIGLISKVIVGTLEGP
                     NIIEKK"
BASE COUNT         1978 a         1553 c         1340 g         2028 t
ORIGIN      
        1 gatagaactg aagttcatct aggctcttga caacggataa ttttgatttt cgaagagtat
       61 tagcttactt agcttccaaa tgccaaatat cttcattgta ctgttgaatc gtacggtctg
      121 atgagaagaa acctgcttta gcgatgttga cgatgacttt ttccaaccag ctgtcgcggt
      181 cttcatagtc agccaacatt ctttccttag tcacaatata gtcttccaaa tctagcaagg
      241 tcatgaagtg gtcattgtgc ttgaggttgt cctgcaaacg ccagagacgc tcatcgatcc
      301 caccagcatg acgaatggta tctgatgtaa taaagtcaat caatggacga atgacttcac
      361 gttcgtagaa ggaatatgga tggtaggtcc tatttgcata gtgttcgata actgtttggc
      421 tatcttgacc aaagatataa atattttcat cacctaccaa ctcatgaatt tccacattgg
      481 ccccatcatc tgtaccgatg gtcaaggctc cattgagcat gaatttcatg ttacctgtac
      541 cagatgcttc cttagatgca agtgaaattt gctcagaaat atccgctgct ggaatgataa
      601 agctagcttc ggttacgttg tagttctcaa tcataactag ctgcaaatgt ggtgccactt
      661 ctgaatcatt tttgataacc tgtgataaga ccagaatcaa gtggatgatg tcttgagctg
      721 ttgtataggc tggagctgct tttcctccaa agaagactgt taacgggcga gtcgggatat
      781 tgcccgcctt gatatccaaa tacttatgga taacatagag gacattcatc tgttgacgct
      841 tgtactcgtg catgcgcttg atttgtacat caaagatgga ttcaggattg acatgtacac
      901 cttgagtttt ggcaatatga cgttgcaact tccgcttgtt gtgttgcttt gtcttttcca
      961 accaagattt caattcctta tcatttttgt aatccaacag cttttctagt tcacttgcat
     1021 cgtgatggta gccatgtcca agtaggccgt ccaggtagct tgccaaactt ggattagcat
     1081 gcatgagcca gcgacggaag gtaatcccat ttgtcttatt attgaatttt tcaggatata
     1141 gctcatagaa agctttcaac tctgaagttt tcaaaatctc tgtatgaagt gccgccaccc
     1201 cattgataga ataaccataa tgaatatcca tgtgtgccat gtgaacacgg ccatattcat
     1261 cgatgatatt gacagcagga tcatccttca cttctttggc acgacgatcc aattctacga
     1321 tgaacggtac caagtgcgga accacatagt tcagaaaatc aagcggccat ttttccagcg
     1381 cctctgacaa gatggtgtgg ttggtataag cagtcatttt cttaacaatt tcaactgcct
     1441 catcaaatga aataccacga agctccaaaa ggcgaatcaa ttctggaata acaagtgatg
     1501 ggtgagtatc gttgatttga acaacagcat aatcaggcag gtcatgtaag tttgacccct
     1561 tagcaactgc ttcgtcaatc aatagttgcg cagcgttaga caccatgaaa tactgctgga
     1621 agatgcggag aacttttcct tcatcggtcg aatcatctgg atagaggaag agggtcaagt
     1681 ttcggaaaat atcttcctta tcaaattcta ttccgtcata aatgatattg tcatcaactg
     1741 aaccaaggtc aaacaagcga agacgattct tacgctccat tttataacca ggtacatcga
     1801 tgtcatacaa cttagatgtc aaggtaaagt tagcaaatgg cacctgataa gaaatcggtg
     1861 actctgttaa ccatgaacgt ggtgtaatcc actcgtttgg cacagcagac tgctgatggt
     1921 attcaaataa ttgtctaaac agtccaaagt gataattcaa gccgacccca tcaccgttaa
     1981 gacctagact agcaattgaa tcaaggaagc aggcagccaa gcgccccaaa cctccattac
     2041 caagagacgg ttcaagctcc atgtcttcaa tagcaatcaa atccttacca gccgccacca
     2101 actggctttt aacctcatca taaagaccaa ggttgattaa attattagac aagagcttac
     2161 caattaagaa ctctgctgaa atatagtaga cctttttctt ttggtcattt gtatacttag
     2221 cttcactctg ctgttttgta aaagcaagca aggcataata gagttcttgg tctgtacaat
     2281 cttcaatgcg tttggcatac atagactgaa caaaattttg taaattaatc atgaggactc
     2341 tcctagatat attaatgtaa tcattcagaa aggtttcgct ttgcctttgt aaacgttttc
     2401 ttaattatac cctatttata aaaaaaagac aagccaactt ccgtacaaat agaaattagt
     2461 tcgtctttta aacgattgca ggttactaca atcagaatta ttcttaatta ttctgcaatc
     2521 agagtttcca aaccaacttt catcatatcc gtgaaagtat tttgacgctc ttctgctgtg
     2581 gtgttttcct ctggattaac caagctatct gaaatcgtca taatggcaag cgtttgaacg
     2641 ccaaacttag cacctaggta ataaagtgca gctgcttcca tttctacagc cttgacacct
     2701 agttgaccta attctaagtt gcgactaaac aattttggag aatagaaact atctgatgat
     2761 aaaacattgc caacatgcgt tgtcatgttg agttctttgg cgatatgata agccttatct
     2821 aaaagatcaa aatcagcgat ttgtggcaaa tcgtactctg gccagtcaat attaatcatg
     2881 cgagagttgg tcgcggctgc ttgcgccaaa accaattcac ggacatggac atcctcattc
     2941 aaagaacctg ctgttcccac acgaattagt tttttcacac catattcgtt aatcaattca
     3001 tgagcataga tagagatgga tggcattccc atacctgttc ccatgacaga aacacggtga
     3061 cctttgtaag taccagtata gccaaacata ttgcggactt cattgaagca aactgcgtct
     3121 tcaagaaaat tttcagcgat aaatttagca cgaagcggat caccaggaag caaaatctta
     3181 tcagcgattt cgcctggttt tgcggaaata tggatagaca tgtaatgtac ctctttgttc
     3241 tattttcgaa tgcttacaac tcagccaaga tagctttcac caagcctttg aagttgccct
     3301 tgatttgctc ggttacttct acaacttctt cgtgattgag ttcagattgg aaaccagctg
     3361 caaagtttgt gatagcagaa atacccaaga ctttcatacc tgagtgagct gccacgataa
     3421 cttctggtac tgttgacata ccgactgcgt gggcacccat agtcttgaag gccaagattt
     3481 cagcaggtgt ttcataggta gggcctgtca caccgagata gacaccatcg tccaacttga
     3541 tacccaattt ttccgcaacg gcatgggctt tttcacggta ttctttagtg taggcattgg
     3601 acatatctgg gaaacgtgga ccaaattctt ccaagttttc gccaatcaat ggattttggc
     3661 cagtcatgtt gatgtggtct gtaatggcca taagggtacc tggaccgtag ccaataccac
     3721 ctgcggcatt agtaacaatc acgccttcac aaccaagagc tttcatgaca cggactggga
     3781 aagtcacgac ctccatagga ttgccttcgt agaagtggaa tcgaccttga agagctaaga
     3841 ccttacgtcc tgccaaatca ccgtacacca acttaccagc atgaccaaca actgttgact
     3901 taccccagtt tggaatatct gcatagtcaa tgacaatggc attttcaact tcttgggcca
     3961 attcacccaa gccagaaccc aaaatcaaac cgaattcagg ttttaccaaa cctttttctt
     4021 ccaagaacgt ttttgtttca ttgatttttg caagtaaact catattttct ccttttataa
     4081 ataatccgac cagtcatatt ctggcatttt ttctttaatg atttcaagcg gacgacagag
     4141 gaaggctgtc tgctctcgct cgataatggg gcgattgaga gtggcaggct cttccactaa
     4201 acgctcaaag atttcttctt ctgacaaggc tagtcgttct tcttccttca atcgagtaac
     4261 tgctgaaggt tgactcccca ttttttccaa gagagctgtc aattcagctc cccttaatgg
     4321 cgtttccaaa taattaaccc acttgacttc catatcctga ctggacagca atattcttaa
     4381 ttttttacac ttgctacagt taggattgta atagaccgtt agcttctcca ttaaaccaat
     4441 tgtgacaaga agctttcgcc aatcatggct gtgtccactc cgaagttttc cgcaacggtt
     4501 gctgaaatgt ccgcaaaatg gcctactggc aaaacaccgc tacctttgaa agccttgctg
     4561 tatgctaaga gtggcacata ttcgcgtgtg tgatctgttc cagcatagga tggatcattg
     4621 ccgtggtctg cagtaatcaa gagcaggtca tcttcacgca tattgtcaat aatctctgga
     4681 atacgtgcat caaactcttg caaacaatcg cggtagcctt cgatattgcg acggtgacca
     4741 tacacagcat caaagtcaac taagtttgtg aaggagaaac cttcctcgaa gtctgataac
     4801 ttcagagtat caatcaacac atcgacaccg tgcatgttgg acttggtgtg acccatatca
     4861 tttgtaatac ctgaaccatt gaaaatatca ctgattttac caactgagta agttggtaca
     4921 cccgcatctg ccagtttgtt gagtactgtt gccttaaaag gtgatactgc atagtcgtga
     4981 cggttggcag ttcgtgagaa attgcctggc tcaccaacgt aaggacgggc gatgatacgg
     5041 cctagaagag ctgggcgttc aagcgtaatg gaacgagcgt attcacagat acggtacaat
     5101 tcatccagcg gaataatgtc ttcgtgggct gcaatctgaa ggactgggtc tgcggaggta
     5161 tagacaatta actccccagt ttccatttgg cgagggccaa agtcatcaat gacagctgta
     5221 cctgaatacg gcttgtttgc ttcgcggatg attttacgac ctgagaattc ttcgattttt
     5281 gtcaaaatct cttcagggaa accatcccag aatgtatcaa atggctcagt aatattgaga
     5341 cccataattt cccagtgtcc agtcatggta tccttaccaa gcgatacttc ttccaacttg
     5401 gtcacgtaac cagttggatg gctttcagca ggaactgtcg caagcgctgt atctcgaggg
     5461 atatttccaa gaccaatctt ggtcatatta ggcacctcaa gcccagcttt ttcagaaatg
     5521 tggccgagag tgtcagattc tgtatctgct acacccgcat tgaaaaattt atctgagtct
     5581 ggtgctgcgc cgattccgac agagtccata actaccaagt gaacacgttt aaatttaggc
     5641 ataattgtct cctttatttg atatagttag attcaaccaa aatcagcgtc caaccaagtt
     5701 gctagactgg aaattatcat taccacacct aaacatttta atggggtgca atacttatac
     5761 agtcaaagct atcggcatca aacttcttaa ccatcaaaac tagatactgt tactcgcttc
     5821 actgcctgct gttcagctat cgctttttca gtacctattg tctgactgct acgaggtaag
     5881 gggatagatt tttgaagagt ctaaacgttc gtgttttcat tataccataa aaaagcctct
     5941 cattgtacca aaatgagaag cgttttcaat ttatttcttt tcgatgatgt tcggtccttc
     6001 tagtgtacca acaattacct tggaaatcaa tccaataaag agaccgtgtt ctacaacacc
     6061 gacggtccga tccagttctt ccgccaatgc atatgtgtct tctattctgc gcaaatcaag
     6121 gtcaatgata aagttttgca tatccgtgat atgtttttct ccattacgca tacgaaaact
     6181 tggacgatac ccttttttct caaatagtcg gaaaagattt tccgcaccgt actgtacaac
     6241 ttccaccggc aacttgaagg ctcctagggt ttgaaccacc ttagactcat ccacaatcca
     6301 gatgcagtcc ttgctattga ctgctaccac tttctccatg agaagcgctg cgccaccgcc
     6361 tttaatccca ttgaagtccc catcaacctc atctgctcca tccaccgtca agtccacata
     6421 ttcgacttcg tcgatatttt ttaggggaat cccaagggac gcagcatgtt ctgccgtagc
     6481 atgagaggtc gttacgcctg tgatttgtaa cccttcttct gcaacccggc ggccaatctc
     6541 ctgcacgaaa tagtatgccg tcgaaccagt ccccagccca acaatcatac cgtccgtcac
     6601 aaactccgcc gccttaatcc caacctgctc ttttaggttt gtcatctttg tctcctttta
     6661 aaaaactgat acaactatta tagcatgaag caagcaagac aaaacaagaa ttttagaaaa
     6721 ttgtggggga gatgtactat cgcttatgat tacgataata taaatcttga gagtacaaaa
     6781 actacaaatg ttatggggat aactaccaga aggttttact gttacttaaa tcttgagagt
     6841 acaaaaacga caaggtcgaa aagaaataca ttgatgttgt tttactgtta cttaaatct
//
LOCUS       DASFSW010000101         6811 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.101, whole genome shotgun sequence.
ACCESSION   DASFSW010000101 DASFSW010000000
VERSION     DASFSW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.101"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            133..1278
                     /gene="nagA"
                     /locus_tag="U1099_001588"
     CDS             133..1278
                     /gene="nagA"
                     /locus_tag="U1099_001588"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEM4867900.1"
                     /translation="MTVFIKAKSIILPESEVESAYLEITDKGQFGRIVTEKPEGEIVD
                     YSDFHLAPGLVDTHIHGYASHDVMDNDFEGIKVISEGLLSCGVTSWLPTTLTDTTENL
                     DAVCETIGTYAGQETGAKIQGIFLEGPFFTEKYKGAQNPKYMSDPSIEKLDNWNSLSK
                     GLVNKIAIAPEREGVKEFIEFANSKAIHTALAHSDATYTQAEAAVEAGANIFVHVYNG
                     MSGLHHREPGMVGAALNLKNVYAEMICDGHHVHPVAAEIVVKARGAEETVLITDCMRA
                     GGMGEGESRLGEFEVVVKDGTARLKESGSLAGSILELIEAVQNVVKWGLVSLPDALRM
                     ASLAPARSVNIDHICGRIAEGRAADFIVVDDAGRLQATYLDGVKRFG"
     gene            1302..2303
                     /gene="lacD"
                     /locus_tag="U1099_001589"
     CDS             1302..2303
                     /gene="lacD"
                     /locus_tag="U1099_001589"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM4867901.1"
                     /translation="MTKLQLSQAKFNHMTRLSNSDQVIAALAIDQRGALKRLLAAAAG
                     GGEFGDDILIDFKKVISSDLTPYASSILLDAEYGVPASELRHADCGFIAAYEKTGYDA
                     STPGRLPDLLPNWSAKRIKELGADAVKILLYYDVDDKPEINDIKHAWVERIGSECIAE
                     DIPYFVEILTYDASDMDVTSREYAALKPHKVNGAMREFSKSRYNADVLKVEVPVNMNF
                     VEGYTDEEPVYTVEEAKAFFKEQTDSTHLPFIYLSAGVSAKLFQETLVFAKEAGSSFN
                     GVLCGRATWKDAVAIFASEGEEAAKAWLADQGRRNVEELNEVLATTACPWTEKVEVK"
     gene            3044..4003
                     /locus_tag="U1099_001590"
     CDS             3044..4003
                     /locus_tag="U1099_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4867902.1"
                     /translation="MGFKKIILSSVALLSAVTLAACSSNSSDSSSVNVGIIQYAEHEA
                     LSSAREGFVEVLEEAGYKEGENLTIDLQNAQGDQANLQTMVEKLAGKNDLNFAIATPA
                     AQAMLNADSETSSVFTAVTDPVEAGLVESLEKPGGTMTGSTDATDVEGQIEMLLKVVP
                     TAKTVGIFYNSSEVNSEVQAEQAKKALEAKGVKVEVTTVTTTNDVQQAITSLAGKVDA
                     IYLPTDNTVASTASTIGDILKEAKVPAMGSDAAVIDAALFTYGVDYHAIGVQAGELAV
                     KILKGEKPADLAVEKPNTAAITVNEEMAKAVGIDAATIEALEE"
     gene            4027..5001
                     /locus_tag="U1099_001591"
     CDS             4027..5001
                     /locus_tag="U1099_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4867903.1"
                     /translation="MYYKLVKKLATISVASMGLLTLAACSSSSEQASSDVVKVGVLQY
                     MEHESLTAAREGFVAELEANGYKEGEKLVLDYQNAQGDQANLQTISEQLIDGNDIVLA
                     IATPSAQSLATVSNETPIVFTAVTDPLSAGLVESIEKPGGLLTGTSDQAPIDKQVELL
                     GQAVPDAKTVGILYTTSERNSEVQVEQAKELLEKAGYKVVVKGITSSNEVQDATTSLM
                     KDVDALFIPTDNTVASTMTMIGELSVEHKVPVIGGSTDMVDEGGLLTYGTNYEALGRQ
                     TAKMAIKIIEGANVSETAVEYPETVSLHVNEEMAQKLGIDTSKLAVSE"
     gene            5056..5949
                     /locus_tag="U1099_001592"
     CDS             5056..5949
                     /locus_tag="U1099_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4867904.1"
                     /translation="MDIILSSISQGLLWSVMAIGVYLTFRILDIADMTAEGSYPLGAA
                     VCATGIVNGVNPLLATFMAMVAGMGAGLISGLLHTKLKIPALLTGIVTLTGLYSINLK
                     ILGKANVALLKQETLVTQLQDFGLTKTNAVLVIGLVFVLAVVGLLTLLLNTQIGLAIR
                     STGDNIPMSEANGINVDNMKIYGYMLSNGLIALCGALLTQNNGYADLNSGTGTIVIGL
                     ASVIIAEVILRNLRLGWRLLSVVLGAVVYRLIILAILEIPSMDADLVKLFSAILLATV
                     LYVPELQKKLNIRRPKLNGNA"
     gene            5959..6759
                     /locus_tag="U1099_001593"
     CDS             5959..6759
                     /locus_tag="U1099_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM4867905.1"
                     /translation="MSTILSIQNIHKTFEAGTVNENHVLRGLNLDVKEGDFISIIGGN
                     GAGKSTFMNSLAGALTVDSGDILLEGKSIKHVSAAKRSKDISRVFQDPKMGTASRLTI
                     EENMAIAYRRGLSRGLGWGVKDSERAILKEALKELGLGLENRMKVDTQFLSGGQRQAL
                     TLMMASLVKPKVLLLDEHTAALDPKNSDMVMELTKKIVESHQLTALMITHNMENAIEY
                     GNRLVMLHRGQIVVDVEGEEKKNLTVQNLMDLFYKNSGEKLTDDEMIL"
BASE COUNT         2040 a         1234 c         1576 g         1961 t
ORIGIN      
        1 ccttctcaca gcatccattt cttataaagt tatggtaatt acttgacatt ttctaataga
       61 agttctacaa tatatttagt atatggttat aactagtttg ataagtatat atatatcaac
      121 aaaggagata atatgacagt atttataaaa gcaaaatcta ttattttacc agagagtgag
      181 gtcgaatcag cctatttgga aattactgac aagggtcagt ttggacggat tgtgactgaa
      241 aagcctgaag gggagattgt tgattattct gactttcatt tagcaccagg gctagttgat
      301 acacatatcc acggctacgc ttctcacgat gttatggaca atgattttga agggatcaag
      361 gtaatttcag aaggtctctt gtcttgtggg gtgacctctt ggcttccaac gaccttaaca
      421 gatacgactg aaaatttaga tgcagtttgt gaaacgattg ggacttatgc agggcaggaa
      481 acaggcgcca agattcaagg tattttctta gaaggtccct tctttacaga aaaatacaag
      541 ggagcacaaa atcctaagta catgagcgat ccgtccattg aaaagttgga caactggaac
      601 agcttatcta aaggtttagt caataaaatt gctattgctc cagaacgtga aggcgtcaaa
      661 gaattcatcg agtttgctaa cagcaaggca attcatactg ccttggctca cagcgatgcg
      721 acctatacgc aagcggaagc ggcagtagaa gctggtgcca atatcttcgt ccatgtttac
      781 aatgggatga gtgggcttca tcaccgtgaa ccaggtatgg tcggtgctgc tttgaattta
      841 aaaaatgtct atgcggaaat gatttgtgat ggacaccatg ttcatccagt tgcggcagaa
      901 atcgtggtca aagcacgtgg agcagaggaa actgttttga ttacggactg tatgcgagct
      961 ggcggtatgg gtgaagggga gtcccgcctc ggtgaatttg aagttgttgt aaaagatgga
     1021 actgctcgtt taaaagaaag tggtagtttg gcaggttcta tcttagaatt gatcgaagca
     1081 gtgcaaaatg ttgtgaaatg gggcttggta tccctacctg atgcccttcg tatggcctct
     1141 ctggcaccag ctcgctctgt caatattgac catatttgtg gtcggattgc agaaggccga
     1201 gctgcagact ttatcgtcgt agatgatgca ggacgcttac aagcgaccta tctagatggc
     1261 gtgaaacgat ttggttaata aaattcctta ggaggaaact aatgacaaaa ttacaacttt
     1321 ctcaggcaaa attcaatcac atgacccgct tgtcaaacag cgatcaggtg attgcggcct
     1381 tggcgattga ccaacgtggt gcattgaaac gcctgcttgc tgctgcagca ggtggtggcg
     1441 aatttggaga tgacattttg attgacttca agaaagtcat ctcaagcgat ttgactccgt
     1501 atgcaagctc cattctcttg gatgcggaat acggtgttcc agcttcagaa ttgcgccatg
     1561 cagattgcgg tttcattgca gcttatgaaa aaacaggtta tgatgcgtcc acaccaggtc
     1621 gtttgccaga ccttttgcca aattggtcag ccaaacgtat caaagaattg ggggcggatg
     1681 ccgtaaaaat cttgctttac tatgatgtag atgacaagcc agaaatcaac gacattaagc
     1741 atgcttgggt agaacgcatt ggtagtgagt gtatcgcaga agatattcct tacttcgtcg
     1801 aaatcttgac ttatgatgcc agcgatatgg atgtgacttc tcgtgaatac gcagccctca
     1861 aaccacacaa ggtcaatggt gccatgcgtg aatttagcaa gtcacgttat aatgccgacg
     1921 tgctcaaggt tgaagtacct gtcaatatga actttgtaga aggttacaca gatgaagagc
     1981 cagtctatac agttgaggaa gctaaggcct tcttcaagga acaaacagat agcactcact
     2041 tgccattcat ttatttgagc gctggcgtat ctgctaaact tttccaagaa acccttgtct
     2101 ttgccaaaga agctggttca agctttaatg gtgttctttg tggtcgggcg acttggaagg
     2161 atgcagtagc catctttgca agcgagggtg aagaagcagc caaagcttgg ttggcggatc
     2221 aaggccgccg caacgtagaa gaactaaacg aggttctagc aactacagcg tgtccttgga
     2281 ctgaaaaagt tgaagtaaaa taattgtttt agtagggtct gggcttaggt tcagactttt
     2341 ttgttacatc aaatatagtc atttatactc ttcgaaaatc aaaatcagtc gttgttgacc
     2401 tgattttgca atccgctctt atatctctca ccttgaacag tccatttact gtgcaagcaa
     2461 tctaggggac tgattcaagt caatgctaga aattgagtta tagctgcggt tgttgacgca
     2521 aaccttgttc cccttatttc caacctctga ctatttgtga aatagttgga gcaagtacta
     2581 aaagtaaact aaaaggctac ataaacatga tgacaggtcg ttttatagct aataatatag
     2641 ggggctattt ttagaaaaat tcgaaaaaac ttgaaaaatc tgaaaattta cgcaatttcc
     2701 tattgacaaa taatttgaaa acagttatga tagttacaaa taaatgagaa agggaaacag
     2761 agatgttaca aatgtgtcaa aatcattcag ctaatcatac ttatttctac ggctatttta
     2821 gatagtgttt gtagacatgg taaacatgga tgcaaacacg ggaacgtaaa gtttgtatct
     2881 atgtggctgt agctattttg acatgtgcct catagatgat acatctaaat ggcacggata
     2941 attcattctg ttttcctata cagatgaaat ataaggaccg tttagagtgt agtctaagcg
     3001 gtcctttctt gtttgctatt atatttataa aaggagaata atcatgggat ttaaaaaaat
     3061 tatacttagt tcagtagcac ttttgtcagc agtaacgctt gctgcatgca gttccaattc
     3121 gtcagattca agttcagtca atgttggtat tattcaatat gctgaacatg aagcgttgag
     3181 ctcagctcgt gaaggctttg tagaggtgct cgaagaagca ggctacaagg aaggtgaaaa
     3241 tctgacgatt gatttgcaaa atgcccaagg tgatcaagca aacttacaga caatggttga
     3301 gaaattggca ggaaaaaatg atcttaactt tgccatcgca acaccggcag cccaagccat
     3361 gcttaatgca gatagtgaaa catcaagtgt attcacagct gtcacagatc cagttgaagc
     3421 aggtttggtt gaatcattgg aaaaaccagg tgggaccatg acgggatcga ctgatgcaac
     3481 tgatgtggaa ggtcaaatcg agatgttgct caaagttgta ccaactgcga agactgtcgg
     3541 tattttctac aattctagcg aggtaaactc agaagttcaa gctgagcagg ctaaaaaagc
     3601 actcgaagca aaaggtgtaa aagtagaagt gacaacagtt acgacgacaa atgatgttca
     3661 gcaagctatt acttcacttg caggaaaagt cgatgctatc tatcttccaa ctgataacac
     3721 agttgcttct acagcttcaa caatcggtga tattttaaaa gaagcaaaag ttccagctat
     3781 gggaagtgac gcggcagtta ttgacgcagc tctatttacc tacggtgtcg actaccatgc
     3841 tatcggtgtt caagctggtg aattggctgt gaagattttg aagggtgaaa agcctgctga
     3901 cttagcagtt gaaaaaccaa atacagcagc tattaccgtc aatgaagaaa tggctaaagc
     3961 tgttggtatt gatgcagcga ctatagaagc attagaagag taatatattt aggagaagaa
     4021 acgtttatgt attataagct agtaaaaaaa ttggcaacta tttcagtagc aagtatgggt
     4081 ttgctaacac ttgcagcttg ttcctcatca tcagaacaag cttcctcaga tgtggtcaaa
     4141 gttggagttc tccaatatat ggagcatgaa tcattgacag ctgcccgtga gggttttgtg
     4201 gcggaattag aagcaaatgg ttataaagag ggtgaaaaat tggttttgga ttatcaaaat
     4261 gcccaaggcg atcaagctaa ccttcaaacc atttcagaac aattaatcga tggaaatgat
     4321 attgtcttgg caatcgctac gccgtctgcc cagagtttag cgactgtgtc taatgaaacg
     4381 ccgattgtct ttactgcggt tacagatccc ttgtcagctg gcttggtaga atccattgaa
     4441 aaaccaggtg gacttttgac aggtacatct gaccaagctc caattgataa acaagttgaa
     4501 ttgctaggtc aagctgttcc agatgctaag accgttggta ttttgtacac tactagcgaa
     4561 cgtaattcag aagttcaggt agagcaagct aaggaattgc tagaaaaagc aggttataag
     4621 gtagtagtaa aaggaatcac atcttcaaat gaagtccaag atgcgacaac tagcttgatg
     4681 aaggatgtag atgctctctt tattccaacc gacaatactg tcgcttcaac catgaccatg
     4741 atcggtgaat tatctgtgga gcataaggtt ccagtaatcg gtggttcaac ggacatggtg
     4801 gatgaaggag gtcttttgac ttacggtacc aactatgaag ctttaggtcg tcaaactgca
     4861 aaaatggcta ttaagattat cgaaggagcc aatgtgtcag aaacagcagt agaataccca
     4921 gagaccgtca gtcttcacgt taatgaagaa atggctcaga aattaggtat cgatacttct
     4981 aagcttgctg tatctgaata agatcggtaa gatagaacct agtattgact acgaatttag
     5041 aaaaaggaga aaatcgtgga tattatttta tcaagtatct cgcaaggctt gctttggtca
     5101 gttatggcga ttggtgttta cttgacgttt cgtattttag atattgctga tatgacagct
     5161 gagggatctt atccgcttgg agctgctgtt tgtgcgacag ggattgtgaa cggagtgaat
     5221 cccttgctgg caacctttat ggctatggta gctggtatgg gtgcaggctt gatttctggt
     5281 ttacttcata caaaactaaa aattccagcc cttttgaccg gtattgtaac cttgacaggt
     5341 ctctactcta tcaacttgaa aatcttaggc aaagccaacg tagccctgct caaacaagaa
     5401 acactggtga cgcaattgca ggatttcggt ttgacaaaaa caaatgctgt tttggtgatt
     5461 ggtctggtct ttgtactagc agttgtaggc ttattgacgc tcttgctcaa tacacaaatt
     5521 ggtctagcta ttcgttcaac aggggataat attccaatga gtgaggccaa tggtatcaat
     5581 gtagacaata tgaaaatcta cggctatatg ttgtcgaatg gtctaattgc cctttgtggt
     5641 gctcttctta ctcagaataa tggctatgca gatctcaact caggtactgg taccattgtt
     5701 atcggtctag catctgtcat tattgcagaa gttatcttgc gtaacttacg cttgggttgg
     5761 agactcctat cggttgttct cggtgcagtt gtttaccgtt tgattatctt agcgattttg
     5821 gaaattccaa gtatggatgc agaccttgtt aaactcttct cagctatcct tctggcaacc
     5881 gttctctatg tgccagaatt gcagaagaaa ttgaatattc gtcgtccaaa attgaatgga
     5941 aatgcttagg aggaagttat gtctacaatt ttatcaatcc aaaatattca taaaaccttc
     6001 gaagctggaa cagttaatga gaaccatgtc cttcgtgggt tgaacttaga tgtaaaagaa
     6061 ggtgatttta tctctatcat cggtggtaat ggtgctggta aatcaacttt catgaactca
     6121 cttgcaggtg ccttgacggt tgattcgggc gatattttgc ttgaaggtaa atccattaag
     6181 catgtatcag ctgccaaacg ctctaaggac attagtcgag tatttcaaga tccaaagatg
     6241 ggaacagctt ctcgattgac cattgaagaa aacatggcta ttgcctatcg tcgtggattg
     6301 tcacgtggtc ttggctgggg tgtgaaggat agtgaacgtg ctattcttaa ggaagccttg
     6361 aaagagcttg gcctgggact agaaaaccgt atgaaggtgg acacgcaatt tctttcaggt
     6421 ggtcaacgtc aggctctgac cttgatgatg gcttcgctgg ttaaaccaaa agttctgctc
     6481 ctggacgaac acactgctgc ccttgatcca aaaaacagcg atatggttat ggaattgact
     6541 aagaagattg tcgagagcca ccagttgaca gctctgatga ttacgcataa tatggaaaac
     6601 gccattgaat atggtaatcg tttggtcatg ttgcaccgtg gtcagattgt tgtcgatgta
     6661 gaaggagaag agaagaagaa cttgactgtt caaaacttga tggacctttt ctacaaaaac
     6721 agcggtgaaa agctaactga cgatgagatg attttataaa aaataagact actgatttca
     6781 tgcaagtcag tagtcttttt tcttatatcg t
//
LOCUS       DASFSW010000102         6780 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.102, whole genome shotgun sequence.
ACCESSION   DASFSW010000102 DASFSW010000000
VERSION     DASFSW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.102"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..981)
                     /gene="neuB"
                     /locus_tag="U1099_001594"
     CDS             complement(<1..981)
                     /gene="neuB"
                     /locus_tag="U1099_001594"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEM4867906.1"
                     /translation="MVYIIAEIGCNHNGDVHLARKMVEVAVDCGVDAVKFQTFKADLL
                     ISKYAPKAEYQKITTGESDSQLEMTRRLELSFEEYLDLRDYCLEKGVDVFSTPFDEES
                     LDFLISTDMPVYKIPSGEITNLPYLEKIGRQAKKVILSTGMAVMDEIHQAVKILQENG
                     TTDISILHCTTEYPTPYPALNLNVLHTLKKEFPNLTIGYSDHSVGSEVPIAAAAMGAE
                     LIEKHFTLDNEMEGPDHKASATPDILAALVKGVRIVEQSLGKFEKEPEEVEVRNKIVA
                     RKSIVAKKAIAKGEVFTEENITVKRPGNGISPMEWYKVLGQVSEQDFEEDQ"
     gene            complement(1503..2909)
                     /locus_tag="U1099_001595"
     CDS             complement(1503..2909)
                     /locus_tag="U1099_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEM4867907.1"
                     /translation="MSKKSIVVSGLVYTIGTILVQGLAFITLPIYTRVISQEVYGQFS
                     LYNSWVGLVGLFIGLQLGGAFGPGWVHFREKFDDFVSTLMVSSIAFFLPIFGLSFLLS
                     QPLSLLFGLPDWVVPLIFLQSFMSVVQGFFTTYLVQRQQSMWTLLLSVLSAVINTALS
                     LFLIFSMENDFIARVMANSATTGVFACVSLLFFYKKIGLHFRKDYLRYGLSISIPLIF
                     HGLGHNVLNQFDRIMLGKMLTLSDVALYSFGYTLASILQIVFSSLNTVWCPWYFEKKR
                     GADKDLLSYVSYYLAIGLFVTFGFLTIYPELAMLLGGSEYRFSMGFIPMIIVGVFFVF
                     LYSFPANIQFYSGNTKFLPIGTFIAGVLNISVNFVLIPTIGIYGAALATTASYLLLLV
                     LHYFVAKKKYAYDEVAISTFVKVIALVVVYTGLMTVFVGSIWIRWSLGIAVLVVYAYI
                     FRKELTVALNTFREKRSK"
     gene            complement(2918..3889)
                     /locus_tag="U1099_001596"
     CDS             complement(2918..3889)
                     /locus_tag="U1099_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide sialyltransferase"
                     /protein_id="HEM4867908.1"
                     /translation="MRRIYICHTMYQILISLLKMDVERDSLMSVDIIGHFPDVREQLQ
                     QHVHLIEETERSFDLYSLIARSKTKERLSLLQSYDEVIIFQDHRQVGHFLNKHRIPYS
                     LLEDGYNFFKDKRVCDLESIQSSVWKRLFYQWYFKPTYLIGSSLYCQSIEVNDLSLVQ
                     FDEAYKPFVEVPRKQLFDQASPEKVQALLQIFGARAIVADEESSQKRLLLLTQPLSWD
                     YHVTEEELLEIYVAGLAPYREDYTIYIKPHPRDGVDYSFLGKAVVLLPQGIPFELFEM
                     AGSIRFDIGMTYSSSALDFLNCFEEKVYLKDTFPLLSKNDILREGIE"
     gene            complement(4248..4505)
                     /locus_tag="U1099_001597"
     CDS             complement(4248..4505)
                     /locus_tag="U1099_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867909.1"
                     /translation="MFKRTVCLIPARSGSKGLSNKNMLFLDGKPMIFHTIDVAIESGC
                     FEKEDIYVSTDSEMYKGGTSINSQNCEFGVTKALLNQHLKF"
     gene            complement(4673..5071)
                     /locus_tag="U1099_001598"
     CDS             complement(4673..5071)
                     /locus_tag="U1099_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867910.1"
                     /translation="MRSKVDTFIATLFGIIGAILIFVNHSIEPFSWFYGWKSTSFLCV
                     PFAYAMLFFMIKYGQKIPATLLSKLGVASYHIYLTQMLYFSVVAPFLAVQFKVSSLNL
                     WNGLFTFLICLFGGYIFYKVDLFMRVRGKR"
     gene            complement(5381..5692)
                     /locus_tag="U1099_001599"
     CDS             complement(5381..5692)
                     /locus_tag="U1099_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867911.1"
                     /translation="MNPTNSRIALFDTIKCIMVLCVIFTHLDWSVEQRQWFIFPYFVD
                     MAVPIFLLLSAYFRTNKWNTKQETLKLKFSSGIKESINMLCLYAIVMAVNVLLSYSRT
                     I"
     gene            complement(5732..6736)
                     /locus_tag="U1099_001600"
     CDS             complement(5732..6736)
                     /locus_tag="U1099_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4867912.1"
                     /translation="MINISIIVPIYNVEQYLSKCINSIVNQTYKHIEILLVNDGSTDN
                     SEEICLAYAKKDSRIRYFKKENGGLSDARNYGISRAKGDYLAFIDSDDFIHSEFIQRL
                     HEAIERENALVAVAGYDRVDASGHFLTAEPLPTNQAVLSGRNVCKKLLEADGHRFVVA
                     WNKLYKKELFEDFRFEKGKIHEDEYFTYRLLYELEKVAIVKECLYYYVDRENSITTSS
                     MTDHRFHCLLEFQNERMDFYESRGDKELLLECYRSFLAFAVLFLGKYNHWLSKQQKKL
                     LQTLFRIVYKQLKQNKRLALLMNAYYLVGCLHLNFSVFLKTGKDKIQERLRRSESSTR
                     "
BASE COUNT         2244 a         1453 c         1122 g         1961 t
ORIGIN      
        1 ttggtcttcc tcaaaatcct gctcactcac ctgccccaag actttgtacc attccattgg
       61 cgaaattcca tttcctggtc ttttgacagt gatgttttct tctgtaaaga cttcgccttt
      121 agcaattgct tttttggcaa caatagattt tctagctaca attttatttc gtacttcaac
      181 ttcttctggc tctttttcaa atttaccaag agattgttcc actatcctca ctccttttac
      241 caaggctgct aagatatcag gagtagcact cgctttatga tctggtcctt ccatttcatt
      301 gtccagagta aagtgctttt caatcaattc agctcccatt gctgcagcag cgatgggtac
      361 ttctgaacca acactatggt ctgaatagcc aattgttaag tttggaaatt cttttttcaa
      421 ggtatgcaag acattcaaat tcaaagcagg gtaaggggtt ggatactcgg ttgtacaatg
      481 caaaatcgaa atatcggtcg ttccattttc ctgcaaaatc ttcaccgctt gatgaatttc
      541 atccataaca gccataccag ttgaaagaat aactttctta gcttgacgac caattttttc
      601 caaataggga agattggtaa tctcaccaga tggaatctta taaacgggca tatctgtgct
      661 aatcaagaag tccaatgatt cctcatcaaa aggtgtcgaa aacacatcaa ctcccttttc
      721 aagacagtaa tcacgcaaat caagatactc ttcaaagctc aattccaaac gacgagtcat
      781 ttcgagctga gaatctgact ctcctgttgt aattttttgg tattctgcct ttggtgcgta
      841 ttttgaaatc aacaaatctg ccttaaatgt ctgaaattta acggcatcca caccacaatc
      901 aacggcaact tctaccattt tccgtgctag atgaacatca ccgttgtgat tacaaccaat
      961 ttctgcaata atataaacca ttttatttcc tttctaaaaa tgactgtaaa tcgctcccat
     1021 aaagtcttga gggagcagct tggttttcct taagtagtaa aagaacacta ataaaaatcg
     1081 ctacttcact tctgatagcc gattagaaaa aatagcccta gatttctttc gaatagtctc
     1141 agggcttgtt gcttctaaag cctgcttctt cacacgatct tcaatagatt ggtggaagtt
     1201 taatctgctt ttctacccaa tttttaggga gatttacctc atgacttctg gcataagcag
     1261 aggcgcatac gtcctttaag aaacattccc ttctttatac ttgattatag cacaattttc
     1321 atggtagaga atacttgaac tttgggtaga tggcagtgat ggtcaattct tatgagcacc
     1381 tctcatttag gacgttttta tatggggacc tctaaaaact aattttctaa aatttaaaat
     1441 gttgatttaa caaggctttc gtaactccaa attcgcaatt ttgggagttt atagaggtgc
     1501 ccttatttag accgtttttc cctgaatgta ttgagggcaa ctgttaattc ctttctaaaa
     1561 atgtaggcat aaacgactag aaccgctatt cctagtgacc aacgaatcca gattgaaccg
     1621 acaaatactg tcatcaagcc tgtatagacg acaacaagag caattacctt aacaaatgtt
     1681 gaaatcgcaa cttcatcgta agcatacttt ttcttagcaa caaaataatg caagactagc
     1741 aacaacagat aggaagcagt cgttgccaaa gcagcaccat aaattcctat cgtcggtatc
     1801 aaaacaaagt tgactgaaat atttagtaca cctgctataa aagtaccaat tggcaaaaac
     1861 tttgtatttc cactataaaa ctggatattg gctggaaaac tataaagaaa tacaaagaac
     1921 accccgacaa taatcatggg aataaatccc atactgaaac gatactcaga tccacctaac
     1981 aacatcgcta attcagggta aattgttaga aatccaaaag tcacaaacag gccaatcgcc
     2041 agatagtaac tgacataact gagcaaatct ttatctgcac ctctcttttt ctcaaaatac
     2101 cacggacacc ataccgtatt caagctcgaa aacacaattt gtaagataga cgcaagtgtg
     2161 tagccgaaac tgtatagggc tacatctgac agtgttagca tcttgccgag catgattctg
     2221 tcaaattgat tgagtacatt atgacctaat ccatgaaaaa taagaggaat cgatatactt
     2281 aaaccatacc gaagatagtc ctttcgaaaa tgaagcccaa tcttcttata gaaaaacaac
     2341 aaggacacac aagcaaaaac accagtcgtt gccgagtttg ccattacacg agcgatgaaa
     2401 tcattctcca tcgaaaagat gagaaataaa gataaagcag tgttaataac agcgctcagt
     2461 accgatagga gtaaagtcca catggactgc tgccgctgca ctaaataggt cgtaaaaaat
     2521 ccttgcacaa cactcataaa actttgcaaa aagataagcg gaacgaccca atcaggcaaa
     2581 ccaaatagga gcgatagggg ctgactgagg agaaaagata gcccaaaaat tggtaaaaag
     2641 aaagcgatag aagagaccat caaggtggat acgaaatcat caaatttctc gcggaagtgt
     2701 acccatcccg ggccaaaagc cccacctaac tgtagaccga taaagagacc aactagcccc
     2761 acccacgaat tatacaagct aaactgccca tatacttcct gagaaatgac acgagtatag
     2821 atggggaggg taatgaaggc taatccctga acgaggatgg ttccaatcgt atagacgaga
     2881 cctgagacaa ctattgattt tttagacatg aatcctccta ttctatcccc tcacgcaaaa
     2941 tatcattttt tgaaagaaga ggaaaagtgt cctttaaata cactttctct tcaaaacaat
     3001 ttaaaaaatc taaagcagac gaactatagg tcataccgat atcaaaacgg atactacctg
     3061 ccatttcgaa caactcaaac ggaatacctt gaggcagaag caccacagcc ttacccagaa
     3121 atgaataatc aaccccatct cgtgggtgcg gttttatgta gattgtatag tcttctcgat
     3181 aaggggcaag acctgctaca taaatctcca acaactcctc ttcggtcaca tgataatccc
     3241 aagacaaggg ctgggtcaat aatagcaatc gtttttgaga agactcctca tccgctacta
     3301 tcgcccttgc tccaaatatc tgcagcagcg cttgcacctt ctctggcgat gcttgatcaa
     3361 ataattgctt tctcggaact tctacaaagg gtttataagc ctcgtcaaat tgtacgagcg
     3421 acagatcatt gacctcaatg gattgacaat agagacttga accaatcaaa tatgttggtt
     3481 taaaatacca ttgataaaag agtcttttcc agacagatga ttgaattgac tccaaatcgc
     3541 acactctttt atccttgaaa aaattataac catcctccaa aagagaatag ggaatccgat
     3601 gtttatttaa aaaatgaccg acttgacggt gatcttgaaa aatgatcacc tcgtcatagc
     3661 tctgtaacaa ggaaaggcgt tcttttgttt ttgatctagc tatcaaagaa tatagatcaa
     3721 atgaacgctc cgtctcctcg attagatgaa catgctgctg cagttgctcc ctgacatctg
     3781 gaaaatgccc gatgatatca acggacatca aactatctct ctcaacgtcc atctttaaca
     3841 aggaaatcag gatctgatac atcgtatggc aaatataaat tcgtctcaat ttttctcctt
     3901 ctacatacag actttaagga taagtaaccc ctttttataa cccctgtaca gcttcgataa
     3961 tgtactgatt cactctttct tgcccttcat cattgaaatg caaaacattt tctgagtccc
     4021 cctgtaagta catattaatg gcttctttta tctgttcgcc agtccgtaac ggtgaggtga
     4081 cttgcagaag aacaaatata caatcttcat aaaatccaga ttcgtaaaaa agtgcgtact
     4141 gacttcattc gatggagtat gataagtaga tagttctttg ggtcttaaca atacattgat
     4201 gcctcttgtc tcgcaaatcc ccttggggac ctctaaaaac taattttcta aaatttaaga
     4261 tgttgattta acaaggcttt cgtaactcca aattcgcaat tttgggaatt tatagaggtg
     4321 ccccccttat acatttctga atccgtactg acatagatgt cttctttctc aaaacaacct
     4381 gattcaattg ccacatcaat cgtgtgaaaa atcatgggtt tcccgtccaa aaatagcatg
     4441 tttttattcg ataagccttt cgaaccagat cgtgctggga ttaaacaaac agttctctta
     4501 aaaattctcc ttgttcagtt aataaatata gctttgaaaa taaaggggga tactggggtt
     4561 tagaaacaga taccactcct ctctcgtcaa cctatttgaa tttctttgtc ggaatattaa
     4621 catgcgaatc tgctaataaa cgaaatggca tctgctaatc tgaaatgagt cattatcgtt
     4681 ttccacgtac tctcataaac agatccactt tgtagaaaat atagccacca aacaggcaaa
     4741 ttagaaaggt aaacaagccg ttccacaaat tcaacgaaga taccttaaat tgcactgcta
     4801 aaaatggtgc gactactgaa aaatacagca tctgggtcaa gtagatatga taagaagcaa
     4861 ctcccaattt tgacaacagt gttgctggaa tcttctgtcc atactttatc ataaaaaata
     4921 gcatagcata cgcaaatggg acgcatagaa aggaagtaga cttccaacca taaaaccagg
     4981 agaagggctc tatagaatga ttcacaaaaa tcagaattgc tccaataatc ccaaataggg
     5041 tcgcaatgaa agtatctacc ttggaacgca catcctgctt tgaaagaaaa aaccaagccc
     5101 tagaacaaaa agataacgaa gtgatattag tctatatata tatgccgtgt tcagccatgt
     5161 tagcaaatat ggcatccact gaaaagttta ctaaaaaaca agtaagcaag cccaaccatt
     5221 tatttttctc gaaaagaaca tacaaaattg gtaataaaaa aactacctga atcaacaacg
     5281 gaacatagta actcccaact ccctggaccc gattctccag aaagtagcca caggacaaat
     5341 gaacggagcg atgaagaacc tgaaaaaggc tttactccta tcagatggtt ctcgaatagc
     5401 tcaataaaac attaacagcc atcacgatag catagagaca aagcatgttt atactttctt
     5461 ttataccact gctgaacttg agctttagcg tctcttgttt tgtattccac ttattcgttc
     5521 gaaaataggc agaaagcaac agaaaaattg gaacagccat gtcaacgaaa tacggaaaga
     5581 taaaccattg acgctgctca acagaccaat ccagatgtgt aaaaataaca caaagtacca
     5641 tgatacattt aatcgtatca aagagtgcta ttctactatt tgttggattc attttaatcc
     5701 ccttttcttt caaccattta ttacaacatt cttaccgagt actactttca cttcttctca
     5761 atctttcttg aattttatct ttccccgttt tcagaaagac actaaaatta agatgaagac
     5821 accctaccaa ataataagca ttcattagta aagcaagtcg cttattttgc ttcaattgtt
     5881 tatatacaat tctaaatagc gtttggagaa gcttcttttg ctgtttgctc aaccaatgat
     5941 tatatttgcc taaaaacaaa acagcaaagg ctaaaaatga acgataacac tctagtaaga
     6001 gctctttatc tcctctactt tcatagaagt ccattcgttc attttgaaat tccagtaggc
     6061 aatggaagcg atggtcagtc atgctagaag ttgtgatact attttctcgg tcaacataat
     6121 agtacaagca ctccttaact attgcaactt tttctaactc atagagcaag cgataagtga
     6181 agtattcatc ttcatgaatc ttaccctttt caaatcgaaa atcttcaaat agttcttttt
     6241 tatagagttt attccaggcc accacaaagc gatgaccatc cgcctctagc agctttttac
     6301 aaacattcct gccgctcaga acagcctgat ttgtaggaag cggctctgct gttaagaaat
     6361 gccccgaagc atctacccta tcataaccag caactgccac aagggcattc tctctctcaa
     6421 ttgcttcgtg taaacgttgg atgaactccg aatgaataaa atcatctgag tctataaaag
     6481 ctaagtagtc acccttggcg cgacttatgc cataattacg ggcatctgat agcccgccgt
     6541 tctctttttt aaaataacga atgcgactat ctttcttcgc atatgctaaa caaatttctt
     6601 ccgaattatc cgtactaccg tcattcacca gaagaatctc tatatgtttg taggtctgat
     6661 ttacaatgct atttatacac ttggatagat attgttcaac attgtaaatt gggacgatga
     6721 tagaaatgtt aatcattatt tttttcttcc cttattaaag tgtttaaaaa aattttaaac
//
LOCUS       DASFSW010000103         6727 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.103, whole genome shotgun sequence.
ACCESSION   DASFSW010000103 DASFSW010000000
VERSION     DASFSW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.103"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..344
                     /locus_tag="U1099_001601"
     CDS             <1..344
                     /locus_tag="U1099_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEM4867913.1"
                     /translation="SFLGEAISTIFHLPIPGSIIGLILLFLALEFKLIRLRHIHTVGN
                     FLLANMTILFLPAAVGIMERFDAIKDYLLPIIIVILGAIFLNILVIGFVVQFVKQKFE
                     GDYIDAEGNHD"
     gene            337..1032
                     /locus_tag="U1099_001602"
     CDS             337..1032
                     /locus_tag="U1099_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEM4867914.1"
                     /translation="MTELFSNPIFGIMLSIAAYLIGMLIYRRFPHPITTPLLVATGLI
                     IVFLKMTGISYKEYYAGGSYLNMLIVPSTVALGIPLYRSFHLMKHHIRSILLGIFVAC
                     IVNTVFTALIAKWFGMDFFLAISLFPKSVTTAMAVGITDKMQGIATVTLVVVVATGIL
                     TSVLGPVFLKLLKIEDPVAVGLALGGTGHAIGTGTAIKYGHTQGAMAGLAIGITGIMY
                     VVISPIVAQIILQ"
     gene            complement(1066..1824)
                     /locus_tag="U1099_001603"
     CDS             complement(1066..1824)
                     /locus_tag="U1099_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEM4867915.1"
                     /translation="MKRKRLLEFEHSFSAYAVRSILASLYLGLGIVISLYTADKLNHV
                     AEGLGKFSYGLMFGWGLLMILYMNAELGTSNMMYMTVASHRKTIPTKTALRMLATCIL
                     FNFVGAVLVCYLVSLTLPYQHVDAHSYLFEATVAKLSKTPLTQFIEGIFANIVVNIGV
                     FTFLRIKDDAGRLISVIFIIFIFAFLGYEHVIANFSLFSLAFFANGGPVEGMTLLSVL
                     SNFLFSGLGNYVGGGLFIGLLYSWLNNQSKLYVD"
     gene            1912..2757
                     /locus_tag="U1099_001604"
     CDS             1912..2757
                     /locus_tag="U1099_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM4867916.1"
                     /translation="MDVTWTVKYITEFLATALLIIIGNGTVANVDLKGTKGNNSGWIL
                     IAIGYGLAVMMPALMFGNVSGNHINPAFTLGLAVSGLFPWAHVAQYIVAQLLGAMFGQ
                     LVVVAVYKPYFLKTENSNHILGSFSTISALDNGTKESRKAATTNGFLNEFVGSFVLFF
                     GALALTKHFFGSELVGKLIEQGYDKTVAETMTAPYTSGSIAVAHLGIGFLVMALVAAL
                     GGPTGPGLNPARDLGPRIVHALLPKSVLGENKADSKWWYAWVGVCAPIIASIAAVALF
                     KFLYL"
     gene            2886..4418
                     /locus_tag="U1099_001605"
     CDS             2886..4418
                     /locus_tag="U1099_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEM4867917.1"
                     /translation="MSDVIAFGYGSSRAEMQLKRLNRHGIIAGATGTGKTVTLKVLAE
                     QLSDAGIPVFLSDIKGDLNSLVAANTKEIDPSRLEKTHYLDYSPVGYPVELWDVLGEN
                     GTPIRMTISELGPVLLTRLLGLNDTQESILNIVFSVADERGLLLIDLMDLRAMLNFVA
                     ENAAELSQYYGNIPARSVGAILRSLVVLEQQGGKIFFGEPSLDIADLMRTAEDGRGVI
                     NVLQATQLFNQPTLYSTVLLSLLSELYEVLPEVGDLDKPKMVFFFDEAHVLFKDAPKV
                     LLEKIELIVRLIRSKGVGVFFVTQNPTDIPDSVAAQLGNRIQHGLRAFTPKELKTVAT
                     VAETFRQEGDEDLAKVIQELQVGEAVVSTLQADGTPSFADRVLIYPPKSMLGTVEPSA
                     LLSVINNSPLMEKYADAVNRESAHEQILAMTEAKEAELIKKAEQAEAEKQAEKEAKAA
                     AKMEEKAQKEAAKAAQKTSQPASRKTDSMMDRFTKNLMSQVGREVGRVVTRGIMGMLK
                     GK"
     gene            4530..5138
                     /locus_tag="U1099_001606"
     CDS             4530..5138
                     /locus_tag="U1099_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEM4867918.1"
                     /translation="MTDQLQALLKDYQPQPLGEKRSYAVFLPLVWSDDQWQVLYEIRS
                     ESISQPGEVSFPGGGIDEGETAEEAAIREVIEELDIQQKQIELLGEIDYLVFGRSTIR
                     CFVGRLHLDWQTLQPNDEVARFFTVPLETLLKTAPVYYQLDSQIVPDCDFPFERLRGG
                     VDYPFSHHKRSVPFYENLPENIWGMTAQFTHCFVEIVKSNLS"
     gene            5429..>6727
                     /locus_tag="U1099_001607"
     CDS             5429..>6727
                     /locus_tag="U1099_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEM4867919.1"
                     /translation="MSKQKVVSSLLLSTVVLGGLAFYSTTTVKAESLEPDVTSVSSSD
                     ATNKLVVSNEEESDFLAEEELEESDAPVETLNEEIKDVTEPSSPEALLQPRAMMSDSM
                     SSGMEEIQVKDEPSDNTAEKVVKQQSDLIQSSSADSFKNGKDKEKLESSVSIDLLSSE
                     NNEIKWKVSFDTSAWSFNYHHGGLYFILPEGLNLKSVKANTNDELFNRFPNKVDSTNN
                     GDGAPFRFFNKEENSDSFNNQWGWSAGQAPNEMINNWKTGNRFSKIYFIDKITDTRQV
                     TYTLTASVTDSNQKSFPLVAVMKSFWYTNSQGTQITSLAGREITLEKEKPLPPKETPA
                     PEAPSTPEKQPESPKEDPKKEDTPEVSPKPEAPTPDAPKQDVPETPDKQPEEMPKVPE
                     APKEDAPAPAPSTPSVPEEKPKEDSKPEVPSAPEAPQQDDV"
BASE COUNT         1997 a         1289 c         1500 g         1941 t
ORIGIN      
        1 tttcattttt aggggaagct atttcgacta tttttcatct tcctattccg ggaagtatca
       61 ttggtttaat cctcctattc ttagccctag agttcaaatt aattcgtctt cgccacattc
      121 atacggttgg aaattttttg ctggctaaca tgaccattct ttttttacct gcagcggttg
      181 ggattatgga gagatttgat gcaattaaag attatttact tcctattatc atcgtcattt
      241 taggcgccat ttttttaaat atcttagtaa ttggttttgt tgttcaattt gtgaagcaaa
      301 agtttgaagg tgactacatt gatgcggagg gaaatcatga ctgaactttt ttccaatcct
      361 atttttggta ttatgttatc aattgcagca tatttaattg gaatgctgat ttatcggaga
      421 tttcctcatc caatcacaac cccactgttg gtagctacag gattgattat tgtcttttta
      481 aaaatgacag gtatttccta taaggaatat tatgcaggag ggtcctatct aaatatgctt
      541 attgtgcctt caacagtagc tctgggtatt cctttgtatc gttctttcca tctaatgaaa
      601 catcacattc gaagtattct tttgggaatt tttgttgcct gtattgtcaa tactgtcttt
      661 actgccctaa ttgcaaaatg gtttgggatg gatttttttc tagcaatctc tctttttcca
      721 aaatcagtca ccactgctat ggctgttggt attactgata aaatgcaagg aatagcaact
      781 gttactcttg tagtcgttgt tgcaacagga attctgacaa gtgttcttgg tccagtcttt
      841 ttaaagctac taaaaattga agacccggta gctgttggtc ttgctttggg aggaacagga
      901 catgccattg gaacgggtac agcgataaaa tatggacata cgcaaggagc gatggctggc
      961 ttggctattg ggattacagg catcatgtat gtggtgatta gtccaatagt tgcgcagatt
     1021 atcttacaat aaaaaactac gattggaatt ccaaccgtag tttttttagt ctacataaag
     1081 tttggattga ttattcaacc aactataaag aagtccgata aagagaccgc cacccacgta
     1141 gtttcctaat ccagaaaaga ggaaattgct tagtacactt aatagagtca ttccctcaac
     1201 tggtccacca tttgcaaaaa aggccaggga gaagagtgaa aagttcgcaa tgacgtgctc
     1261 atatcccaag aaagcaaaga taaagatgat gaaaataacg gaaatgagac gcccagcgtc
     1321 atctttgatg cgtaagaagg taaatacacc gatattcacg acaatattag caaaaatacc
     1381 ttcaataaat tgggtcaaag gtgtctttga aagcttggcc accgttgctt caaataggta
     1441 gctgtgagca tcaacgtgct gataaggcag agtcagtgag accaagtaac aaacaaggac
     1501 cgcaccgaca aagttgaaaa ggatacaggt tgccaacatc ctgagggctg ttttcgtagg
     1561 aatcgttttt cgatggctgg ctactgtcat atacatcatg ttggatgttc caagttcggc
     1621 attcatatat aaaatcatga gtagccccca accaaacatc aacccatagc taaatttccc
     1681 gagtccttct gcgacatggt tgagtttgtc tgctgtataa agagaaatga caatccccaa
     1741 tcctaaataa agactggcta gtatcgatcg tacagcgtag gcagaaaaac tgtgttcaaa
     1801 ttcgagtaaa cgttttcttt ttatcgaatg gctttacttt gtttattata cttgataaaa
     1861 tggttgttgt ccaaaaaaaa ttcaactttt gaaaggaaat ataagaaaaa tatggatgtt
     1921 acatggacag tgaaatatat cactgaattt ttagcgactg cactccttat tatcattggt
     1981 aatggtacag ttgcaaacgt tgatttaaaa ggcacaaagg gaaacaactc tggttggatt
     2041 ctcattgcga ttggctatgg tttggcagtt atgatgccag cattgatgtt tggtaacgtt
     2101 tccggtaacc atatcaaccc agccttcaca cttggtttgg ctgtttcagg tctcttccct
     2161 tgggctcacg ttgctcaata cattgttgcc caattgttag gtgcaatgtt tggtcagtta
     2221 gtggttgtag cagtgtataa accttacttc ttgaaaactg aaaattctaa ccatattctt
     2281 ggctcattct caactatcag tgcgcttgat aacggaacga aagaaagccg taaagctgcg
     2341 acaactaatg gtttcttgaa tgagttcgtt ggttcatttg tattgttctt cggtgccctt
     2401 gctttgacca aacatttctt tggttcagag ttggttggta aattgattga acagggctat
     2461 gataaaacag tcgctgagac aatgactgct ccatatactt caggttcaat tgcagttgct
     2521 cacttaggca ttggttttct agttatggca cttgttgcgg ctcttggtgg tccaacaggt
     2581 cctggtctta acccagcgcg tgaccttggt ccacgtatcg tccatgccct tcttccaaaa
     2641 tctgttcttg gtgaaaacaa ggctgattca aaatggtggt atgcttgggt tggtgtatgt
     2701 gctccaatta tcgcatcaat tgccgctgtt gcacttttca aattcttgta tctataatat
     2761 agagaacccg tttcgtcggg tttttctttt tctcttggta tatcctatat aaagttgtct
     2821 gctctgctat tttcctaaaa gttatgctac aataaggata gaaaaatcgg aaaagaggct
     2881 agaatatgtc agatgttatt gcttttggct atggaagcag tcgagcggag atgcagctca
     2941 agcggctcaa tcgtcatgga atcatcgcag gtgcgacagg tacagggaag accgtgacct
     3001 taaaggtctt ggcagagcag ttgagtgatg cgggaattcc cgtctttcta tcggacatca
     3061 agggtgattt gaatagtttg gttgcggcca ataccaagga gattgatcct agtcgtctag
     3121 agaagactca ttatcttgac tatagcccag tcggttatcc agttgagttg tgggatgtct
     3181 tgggggaaaa tgggactcct atccgcatga ctatttcaga gctgggacct gtcctactga
     3241 cacgtttgtt gggcttgaat gatactcagg agtccatctt gaatatcgtt ttcagcgtgg
     3301 cagacgagcg tggccttctc ttgattgatt tgatggacct acgggctatg ctcaattttg
     3361 tggctgaaaa tgcggcagag ctgagccaat attatggaaa tattccagct cgttcagttg
     3421 gtgccatctt gcgtagcttg gttgttttgg agcaacaagg tggtaagatt ttcttcggtg
     3481 agccaagcct tgatattgct gatttgatgc gtacggcaga agatggccgt ggggttatca
     3541 acgttctcca agctactcag ctattcaatc aaccaactct ttattcgaca gtcctgctta
     3601 gcctcttatc agaactctat gaagtcttgc ctgaggtggg cgatttggac aagcctaaga
     3661 tggtcttctt ctttgacgag gctcatgttc tcttcaaaga tgctccaaaa gttcttcttg
     3721 aaaagattga attgattgtc cgtttgattc gttcaaaagg tgtaggtgtc ttctttgtaa
     3781 cgcagaatcc gacggatatt cctgatagcg tggcagccca gttgggcaac cgcatccaac
     3841 atggtcttcg cgcctttaca ccaaaagagc ttaagacagt cgcgacagtt gccgaaacct
     3901 tccgtcaaga aggtgatgag gatttggcca aggtcattca ggagttgcag gtaggtgaag
     3961 cggttgtgtc tactctgcaa gctgacggga caccaagctt tgcggatcgg gttttgattt
     4021 atccaccaaa gagtatgctg ggaacggtgg agccaagcgc cctcttgtca gttatcaata
     4081 attctccttt gatggaaaaa tatgctgacg cggtcaatcg tgagtcagcc catgagcaga
     4141 ttttagccat gacagaggct aaagaagccg aactcattaa aaaagcagag caggccgaag
     4201 cggaaaaaca ggcagaaaaa gaagctaagg cagccgcaaa aatggaagag aaagcccaga
     4261 aagaagcagc taaagctgca caaaaaacta gtcagccagc cagtcgcaaa acagattcca
     4321 tgatggatcg ctttaccaag aacctgatga gtcaggtcgg acgggaagtt gggcgcgtgg
     4381 taactcgtgg cattatgggc atgttaaaag ggaaataaac gactagactg agaaggaaac
     4441 ttctcagttt tttatctaga caaagttctt gccagatgcc acaaaattcg ttaaaataaa
     4501 cgtaaatcac tagttagtaa ggagttaccg tgactgatca attgcaggca ttattgaaag
     4561 actatcagcc ccagccatta ggtgaaaaac gttcttatgc agttttcttg cctctagtat
     4621 ggtcagatga tcagtggcaa gttctatatg aaattcggag cgagtccatt tctcagccag
     4681 gagaagtttc ctttccaggt ggcggaattg atgaaggcga gacagcagaa gaagctgcca
     4741 ttcgtgaggt tatcgaagaa cttgacattc aacagaaaca aattgagttg ctgggagaaa
     4801 ttgattatct cgttttcggt cgctcgacga ttcgctgttt tgtcggtcga cttcacttgg
     4861 actggcagac ccttcaaccc aatgatgaag tggctcgatt ctttacagtt ccactagaaa
     4921 ccttgctgaa aacggcccct gtctattatc aactggattc acagattgtt ccagattgtg
     4981 actttccctt tgagcgtttg agaggtgggg ttgactatcc tttcagtcat cacaaacgct
     5041 ccgttccctt ttatgaaaat ctaccagaaa atatctgggg aatgaccgct caatttaccc
     5101 attgttttgt ggaaatagtg aagagtaatc tatcgtgaaa tgataggtta tttttaggag
     5161 gaaaaaccag cctttgcgat atgtatcagc gaaaaaaatg gagataaagc tcaatgtgag
     5221 taatgtatag aaaaacacaa aaaaaacaga tgcatggcag ataaaagata gctatggctg
     5281 gtatacttgg tacggtccat atttttaagt accatcatct atccaggaat gcgacaatat
     5341 tcatgtaaac gcattcagtg gcttgttgtc atttccatgt agatgagtgg gaaaatgtat
     5401 aaatctatat tttaagaagg agccaattat gagcaagcag aaagttgtgt ccagtttatt
     5461 attaagcaca gtcgtattag ggggattagc cttttattca acgacaacgg ttaaagcaga
     5521 atcgctagaa ccagatgtta catcagttag ttcaagcgat gctactaata aactggtagt
     5581 atcaaatgaa gaggaaagcg actttttagc cgaagaagag cttgaagaga gcgatgctcc
     5641 agtagaaact ctaaatgaag aaatcaaaga tgtcacagag ccatcttcac ctgaagcact
     5701 tctccaacct cgtgcgatga tgagcgattc tatgagtagc ggtatggaag aaattcaggt
     5761 gaaagacgag ccttcagata atacggcaga aaaggtagtg aagcaacagt cggacctaat
     5821 tcaatcatcg agcgcggact cctttaagaa tggaaaggac aaagagaaac tcgagtcttc
     5881 agtgtctata gacttgttga gttcagaaaa caatgagata aaatggaaag taagttttga
     5941 cacaagtgca tggtctttta attatcatca tggaggtctt tattttattc ttccggaagg
     6001 tttgaattta aagagtgtta aagctaatac taatgatgaa ttatttaatc gattccctaa
     6061 taaggtagat agtactaata atggtgatgg agcaccattc cgtttcttta ataaagaaga
     6121 aaacagcgat agctttaata atcaatgggg gtggtcagca gggcaagctc ctaatgaaat
     6181 gattaataac tggaaaacag gaaatcgttt ttctaagatt tactttatag ataaaataac
     6241 agatactaga caggtaacct atactttaac tgctagtgtg actgattcaa atcaaaaatc
     6301 attcccactt gtagcagtga tgaagagctt ttggtataca aactcgcaag gaacgcagat
     6361 tacttcatta gctggtcgtg agattaccct tgagaaagaa aaacctctac cacctaaaga
     6421 gactccagcg ccagaagctc cgtcaactcc tgaaaagcaa ccggaatctc caaaggaaga
     6481 tcctaagaag gaagatactc cggaggtttc accaaaacca gaagcgccaa caccagacgc
     6541 accaaaacag gacgttccag aaactccgga taagcaacca gaagaaatgc ctaaagttcc
     6601 tgaagcaccg aaggaagacg ctccagctcc agcgccatcg actccatcag taccagaaga
     6661 aaaaccaaag gaagattcga aaccagaagt gccatcagca ccggaagcac cacaacaaga
     6721 cgatgtc
//
LOCUS       DASFSW010000104         6496 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.104, whole genome shotgun sequence.
ACCESSION   DASFSW010000104 DASFSW010000000
VERSION     DASFSW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.104"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            179..1360
                     /locus_tag="U1099_001608"
     CDS             179..1360
                     /locus_tag="U1099_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEM4867920.1"
                     /translation="MVFSVRTFNNINQIGLKELGNKFQIDGDHAANPDAFIIRSENLH
                     GFDFPENLKAIARAGAGTNNIPIEEATEKGIVVFNTPGANANAVKEAVIASILLSARD
                     YIGATAWANTLSGDDVPKQVEAGKKQFAGTEISGKTLGVIGLGAIGARIANDARRLGM
                     NVLGYDPYVSVETAWTISSHVKRVDDLKEIFTNADYITVHVPLTDKTRGLFNADSFGQ
                     MRKGTTLINFARGELVDNADLFEAIEAGVIKNYITDFGTEEVLNKDNIIVFPHVGGST
                     EEAELNCAIAAGQTIRRFMETGEIINSVNFPNVKQFLDAPYRITLINKNIPNMVATIT
                     TAVSELGINIDNIINKSKGDYAYTLLDLDEADKVKIEQLVAKFEATDAIVKVRVIQNK
                     N"
     gene            1363..1869
                     /locus_tag="U1099_001609"
     CDS             1363..1869
                     /locus_tag="U1099_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEM4867921.1"
                     /translation="MLYKQVYHSPLGPMSLIASDKGLRGAWFEGQKYFERGLNEKAIL
                     ASHPILDQTVRLLDQYFSGVQVDFSNLPLEAVGTNFQEHVWQLLKMIPIGQTTTYGQL
                     AQQLGLRSGQAVGGAVGRNPYSIIVPCHRVLNQKGQLTGYAGGLDKKIWLLRHENPEF
                     EVKDDFIL"
     gene            1853..2206
                     /locus_tag="U1099_001610"
     CDS             1853..2206
                     /locus_tag="U1099_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEM4867922.1"
                     /translation="MILFYEYPKCSTCRAAKAELKSLGLEFEAIDIKSTPPSAEELKA
                     WMEATGLELKKYFNTSGNSYRELGLKDKFDSLSLEQALNLLANDGMLIKRPLLIQDGK
                     ILQIGYRTKYENLGL"
     gene            complement(2223..3122)
                     /locus_tag="U1099_001611"
     CDS             complement(2223..3122)
                     /locus_tag="U1099_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM4867923.1"
                     /translation="MDHKIIERKSLTVSSVMNGLSGLAGIAVYIMTDLNALLLDGAFS
                     LIAFISSLVAVYISKNSHRKTETFPQGLYFLEPLYAIMKSLATLLLLVFAVLETSATA
                     FAYFVHGIGHQMTTGPVLPYTITMFFVCMGLYLYNRHMSTKVNHLSTIILAEAKGNLV
                     DGLISGAIGIAILLLYLVPINSWFGFLHYTGDFFLTLALAIISFKEPWNLLVASFKEL
                     ANGTIHFPEIHDSIYQLLEPYLKEDSNDVEIHIFKQGMKIRVKINLHNIHHEIIQKLL
                     EDKPQMLYLLKKHHEYISIEYAL"
     gene            complement(3422..4249)
                     /gene="xth"
                     /locus_tag="U1099_001612"
     CDS             complement(3422..4249)
                     /gene="xth"
                     /locus_tag="U1099_001612"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEM4867924.1"
                     /translation="MKLISWNIDSLNAALTAESPRALLSRAVIDTLVTEDADIIAIQE
                     TKLSDKGPTKKHLEILESYFPGYANTWRSSVEPARKGYAGTLFLYKNHLSPTITFPEI
                     GAPTTMDSEGRIITLEFDDFYVTQVYTPNAGDGLKRLADRQIWDVQYADYLAKLDSHK
                     PVLATGDYNVAHKEIDLANPASNRQSPGFTDEERQGFTNLLAKGFTDTFRHLHGDVLN
                     AYTWWAQRSRTSKINNTGWRIDYWLVSDRIADKVSKSDMIDSGARQDHTPIVMEISF"
     gene            4405..6066
                     /locus_tag="U1099_001613"
     CDS             4405..6066
                     /locus_tag="U1099_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867925.1"
                     /translation="MKRKLFVFAWLALVLEMLIYYWYQLPALNILSVDFWIFFLQSVG
                     LVWLILSMFSSKGAFQKVTKISGRQRQVDTYQIKTSQLPAYLKWLGRIWVLVVLGLLG
                     LGLINSRVFRAKDYAAVISVKDADFKADFPETDISKLALLDRASAEKIGDTYLGTIDK
                     VSQFGISDDYRQITIGQQPFRVSPLEYKNFWKWLSNHQDGIGYYVKVNQTTGKAELAK
                     LSKAMHYSDSEFLLNDTLRHLRLQYPTTIFGKPSFEVDDQGNPYYIATIYEPKFGLSS
                     NDPIGAIVLDAVTGESKKYSLEDIPEWVDRVYSANNVISRVDDHYTYQNGFWNTIFSQ
                     TGVKNTTDSYNYISIGSDIYLYTGITSATADSSNLGFILVNMRTREITNYKLASATET
                     AAQESAEGEVQEKGYKATAPSLVKLADTAYYLVSLKDDAGLVKSYALVDAEDYQQVTV
                     NNDIALLISQVTGTDASSLSGLTVTENGEVGEQTEVISGKVEALASQIIGGATIYYIE
                     SEGKIYKVKATEDSTDRLPFIKVGDHFSGQLDKKNYLKNFKVNPE"
     gene            6271..>6496
                     /locus_tag="U1099_001614"
     CDS             6271..>6496
                     /locus_tag="U1099_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM4867926.1"
                     /translation="MRRHLGLVDLIFLGIGSMVGTGIFTVTGLAAAQYAGPALIISIV
                     IAAISVGLTALFYAEFASRIPTNGGAYGYLY"
BASE COUNT         1925 a         1168 c         1469 g         1934 t
ORIGIN      
        1 agttcacctt atctccaacc tcaaactgtc tcccagacag tttgagctgt gcgggggtgg
       61 gagtgaaaca gtctgggaat agactgtttc agctcaacaa atagaaataa agacttgttg
      121 acgaactctt ctaggagtgg ttgagttctt tcccactccc ctttttaggt aaaaataaat
      181 ggtatttagc gtacgtactt ttaacaatat caatcaaatt ggtcttaagg aattaggcaa
      241 taaattccag attgacggag atcatgcagc aaatccagat gcctttatta ttcgatctga
      301 aaatttgcat ggatttgatt ttcctgaaaa tctaaaagct attgcccgtg ctggtgcagg
      361 gactaacaat attccaatcg aagaggcaac agagaaaggt attgttgtct ttaacactcc
      421 aggtgccaat gccaatgccg taaaagaggc agtcattgct tccattcttt tgtcagcacg
      481 tgactatatc ggagcgactg catgggcaaa tacattgtca ggagatgatg tacctaagca
      541 ggttgaagca ggtaagaagc agtttgctgg gacagaaatt tcaggtaaga ctcttggtgt
      601 tatcggactt ggtgctatcg gtgcgcgtat tgccaacgat gcccgccgtt tggggatgaa
      661 tgttctcggt tacgaccctt atgtatcagt agaaactgca tggaccattt caagccatgt
      721 caaacgtgtg gatgacttga aagaaatctt taccaatgcg gattacatta ctgttcatgt
      781 tcctttgaca gataagacac gaggactctt caatgcggat agctttggtc aaatgagaaa
      841 aggaacaacc ttgataaact ttgcccgagg tgaattggtt gataatgctg atttgtttga
      901 agcaattgag gcaggagtta tcaagaacta tatcacggat tttgggacag aagaagtgct
      961 caacaaggac aacattatcg tcttcccgca cgttggtggg tcaacagagg aagcagagct
     1021 caactgtgcc attgctgctg gtcagaccat tcgtcgtttt atggagacgg gtgaaattat
     1081 caactcagtt aacttcccaa atgtgaaaca attcctagat gcaccgtatc gtattacctt
     1141 gattaataag aatattccta atatggtggc aacgatcaca acggctgtat ctgagttggg
     1201 aatcaatatc gataatatca tcaataagtc taaaggtgac tatgcctata ctttacttga
     1261 tttggatgaa gcggataaag tgaaaattga gcagttggtt gctaaatttg aagcaacgga
     1321 tgcaattgtc aaagtgcgtg tcattcagaa taaaaactaa tcatgctgta taaacaagtc
     1381 tatcacagtc cacttggccc catgtcatta attgctagtg ataagggatt gagaggggcc
     1441 tggtttgagg gacaaaaata ttttgaacga ggcttgaatg agaaagctat tttagcctcc
     1501 catcccattt tagaccagac agttcgattg cttgaccagt atttttcagg ggttcaggtg
     1561 gacttttcaa accttccatt agaagctgtg ggaacaaatt tccaagagca tgtctggcaa
     1621 ttattgaaga tgattccgat tggtcagacg actacctatg gacaactggc tcagcaactg
     1681 ggccttcgtt ctggtcaggc agtaggagga gcagttgggc gtaatcctta ttcgattatt
     1741 gttccttgtc accgagttct caatcagaaa ggacagttga caggttatgc aggtggactg
     1801 gataagaaaa tctggctctt gcgacatgag aatccagaat ttgaggtgaa agatgatttt
     1861 attttatgag tatccaaaat gttcgacttg ccgagcagca aaggcggagc tgaagagttt
     1921 gggattggaa tttgaagcta ttgacattaa gtcaacaccg cctagtgctg aagagctaaa
     1981 agcttggatg gaggcgacag gactagaatt gaaaaagtat tttaatactt ctgggaatag
     2041 ctatcgtgaa ctaggtttga aagataagtt tgatagtctt agcttggagc aggcacttaa
     2101 tcttctagct aatgatggta tgctgattaa gcgtccccta ctaattcagg atggtaaaat
     2161 cttacagatt ggctatcgaa caaaatacga aaatctcggt ttataagccg agattttgtt
     2221 tattatagcg catattctat tgaaatatat tcatgatgtt ttttcaaaag atagagcatc
     2281 tgaggcttat cttctaagag tttttggata atttcgtgat ggatattgtg gagattgatt
     2341 ttaacgcgaa tcttcatgcc ttgtttgaaa atatggattt cgacatcatt gctatcttcc
     2401 tttaagtaag gctccaatag ctgatagata gaatcatgta tctcagggaa atggattgtt
     2461 ccgttggcaa gttccttgaa agatgccact aataagttcc aaggttcctt aaatgaaatg
     2521 attgctagtg ctagtgtcaa aaagaagtca ccagtataat gcaggaagcc gaaccaagag
     2581 ttgataggaa caagatagag cagaagaatt gcaattccaa ttgcacctga aatgagtccg
     2641 tctacaaggt tgccttttgc ttctgctaat atgatagtag acagatgatt gactttggta
     2701 ctcatatggc ggttgtatag gtaaagcccc atacagacaa aaaacatggt tatggtataa
     2761 gggagaacgg gacctgtagt catttgatga ccgattccat gaacaaagta agcaaaggct
     2821 gtcgcgctag tttcaagtac agcaaataca aggagtagta gagttgcgag agatttcatg
     2881 atagcgtaga gaggttctag aaaatacagt ccttgaggaa aagtctcggt tttccgatgg
     2941 ctattttttg atatgtaaac tgcaactaag gacgaaataa atgctattag agaaaaggct
     3001 ccgtctagga ggagggcgtt taggtcagtc atgatataaa cggcaatacc tgctagtcca
     3061 gataaaccgt tcataacaga ggaaacagtt agagatttgc gttcgataat tttgtggtcc
     3121 ataatccctc cttttaatgt atatctatat atttatttta ccacaaaatg acaccgtttt
     3181 caaattgagc ggattaaaac aaggtagaga ggtagagtga taagaaataa aggctcaaaa
     3241 cccttgcaat agacctacgg gttttaagcc tttaatatct gttatctgat aattgaaatc
     3301 aggttatact cttcgaaaat caaaatcaga cgttgttgac ttgatttgat gaacttcagt
     3361 tctatctgca tctgcgtcgc ctagtctgat tttgattttc attgagtatt aatatccact
     3421 gttagaatga aatctccatc acaattggtg tatggtcctg acgagcaccg gagtcaatca
     3481 tatctgactt acttactttg tcggcgatgc ggtcgctgac aagccagtaa tcaattctcc
     3541 agcctgtgtt gttgatttta ctggtgcggc tacgttgtgc ccaccaagta taggcgttga
     3601 gaacatcacc gtgcaagtgg cggaaggtat ctgtaaaacc ttttgctaga agatttgtaa
     3661 aaccttgacg ttcttcatct gtaaaccctg gagattgacg gttacttgcg ggattagcaa
     3721 ggtcaatttc cttgtgagca acgttgtagt cacctgttgc aagaacaggt ttgtggctat
     3781 caagttttgc taagtagtca gcatactgta catcccagat ttgacggtca gcaagccgtt
     3841 tcaagccatc accagcattt ggcgtataaa cttgtgtgac atagaagtca tcaaattcta
     3901 gcgtgatgat acgcccttca gaatccatgg ttgttggagc accaatctca gggaaggtta
     3961 tggttggact tagatggttt ttgtagagga agagtgtacc agcatagcct ttgcgagctg
     4021 gttctacaga tgaacgccag gtattggcat atccagggaa gtagctttcc aaaatttcta
     4081 gatgcttttt agtcggtccc ttatctgaca attttgtttc ctgaatggcg ataatatcag
     4141 cgtcttctgt cacaagtgta tcgatgactg cacgtgataa tagggcgcga ggagactctg
     4201 cagtcaatgc agcgttaaga gaatcaatat tccaagaaat gagtttcata attttccttt
     4261 tctagtctgt tactgtttta cattatacca aaaataaaag ttgcaggcga atatggaaac
     4321 ttgaaaaaaa ttttataatt cttgtattat aacattaaaa ttgtgataca atagaactaa
     4381 taaatctgtg tcaaggaggt tattatgaaa agaaaattat ttgtttttgc ctggttagcc
     4441 ctagttttgg aaatgctaat ctactactgg taccagttgc ctgctttaaa tattttgagt
     4501 gtggacttct ggatattttt ccttcaatct gtcggattgg tctggctcat actttccatg
     4561 ttcagctcca aaggtgcttt tcaaaaggtg accaagattt ccggtcgcca gagacaagta
     4621 gatacctatc agattaaaac ctcgcaactg ccagcttact taaaatggtt gggacgaatt
     4681 tgggtattgg ttgtcctggg tttgttgggg ctggggctta tcaactcacg tgtatttcgt
     4741 gctaaggatt acgctgctgt cataagcgtt aaggatgcgg attttaaagc agattttcct
     4801 gaaacagaca tatccaaatt ggccctccta gaccgcgcct cggccgaaaa aatcggtgat
     4861 acttatctag gtaccattga caaggtctcc cagtttggca tttcggatga ttatcgccag
     4921 attactatcg gtcagcaacc gttccgtgta tcgcctttgg aatataagaa tttttggaaa
     4981 tggctgagca accatcaaga tgggattggt tactacgtga aggttaatca gacaacaggg
     5041 aaggcagaac ttgccaagtt gagcaaagcc atgcactatt cagattccga atttttgctg
     5101 aatgatacct tacgtcatct tcgcttgcaa tacccgacaa ctatttttgg gaaaccatcc
     5161 ttcgaagtgg atgaccaagg aaatccatat tatattgcaa cgatttacga gccaaaattt
     5221 ggactttcat caaatgatcc gattggtgct attgtattgg atgcagtaac aggggaaagt
     5281 aagaagtata gtctggaaga tattccagag tgggtagacc gtgtttattc ggcaaacaat
     5341 gttatcagcc gtgtagatga ccactatact tatcaaaatg gtttctggaa taccattttc
     5401 agtcaaactg gtgtgaaaaa tacaacagac agctataatt atatttctat cggctcagat
     5461 atttatctct acacaggtat cacctcagca actgctgatt catccaacct tggatttatt
     5521 cttgtcaata tgagaactcg tgaaattacc aactacaaat tggcttcggc gacggaaaca
     5581 gcagcgcaag agtccgcaga aggtgaagtg caggaaaaag gctataaagc cactgctcca
     5641 agcctggtta agttggcaga tacagcctac tatttagtat ccttgaaaga tgatgccggc
     5701 ttggttaaat catacgctct tgttgatgcc gaagattatc aacaggttac tgttaacaac
     5761 gatattgcgc tcttaatttc tcaagtcaca ggaacggatg cttctagcct ttcaggattg
     5821 acggtgactg aaaatggaga agttggtgaa cagactgagg ttatcagtgg aaaagtagaa
     5881 gctttagcga gtcaaataat tggcggtgcg acaatctact atattgagtc tgagggcaag
     5941 atttacaaag tcaaagcaac ggaagatagt acagatcgat taccatttat caaagtagga
     6001 gatcacttta gcggtcagtt agataagaaa aattatctta aaaactttaa ggttaatcca
     6061 gaataatcat aagaaagagt gaggtagaaa agccttgctc ttttgcttgt cgtcacccat
     6121 gcgaaatatt tcaacttatg gtataatggg gcgttgtggt tctttgacag gaatccgtag
     6181 attattctgt cgagtatcca tattcttatt ctgaaggagt attttgtgag tttatttaga
     6241 aaaaaacaag cagtcggtcg ccatagtcaa atgcgtcggc atttaggctt agttgatttg
     6301 atatttttag gtatcggttc gatggttgga acaggtatct ttacggtaac tggtttggca
     6361 gcagcccagt atgcagggcc agctctgatt atctcaattg ttattgcagc aatttcagtc
     6421 ggactaacag ccttgtttta cgcagaattt gcctcgcgta taccgactaa cggtggtgcc
     6481 tatggttatc tctact
//
LOCUS       DASFSW010000105         6449 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.105, whole genome shotgun sequence.
ACCESSION   DASFSW010000105 DASFSW010000000
VERSION     DASFSW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.105"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1591
                     /locus_tag="U1099_001615"
     CDS             <1..1591
                     /locus_tag="U1099_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEM4867927.1"
                     /translation="EPKAERNYMIIDSNYESWAMKQKTADLLLFNKEKSLVGLDHIAY
                     VEPTYITENKKNHLLDIYKALKGKVEEAKATDTDDKDDKQEGYNRVLQFVNSDIDKLS
                     TLSKITEEEFKALENSMDLARVFLGQMNAKAGKEFSEGETYQSYLKNRQKNNTNSDDD
                     RNRNELQENQANSDEVSQNSKDASAPSVNSAQHSEELEGTPSTQETISAAPSQQTPVA
                     PKALQAKTELEDKTETSSGSFERESNEVSANTVLSPEITKNTGSLASVESRVEEAVDR
                     TTPSEKVSAESEEVEKTIVSEVSLDASLANENKNSDAQEDTPRLEKSAEVVTTSQEVA
                     EAPVSAPQVSSEIPTSPETNSTEAVTTSQEMAESRVSAPQVSFVSPTLEVPKNERLDE
                     KADATMPNGVGANTHEAGSVPTSDIYVQDAGSDTVPQPYSLSATLFEEAISTVEPVGV
                     ATSSQERSAVAGKVKVPASLERSNNSVEEEKVVDSNATIEKRATEKKEVLTSENVLNS
                     LVTIWVALSTSFFMRYFSRGK"
     gene            1911..4436
                     /gene="secA"
                     /locus_tag="U1099_001616"
     CDS             1911..4436
                     /gene="secA"
                     /locus_tag="U1099_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEM4867928.1"
                     /translation="MVTNVLRSLIENDKGELRKLEKMADKVFSYADEMEALTDEQLQA
                     KTAEFKERYNNGESLDDLLYEAYAVVREGARRVLGLYPYKVQVMGGIVLHNGDVPEMR
                     TGEGKTLTATMPVYLNALSGQGVHVVTVNEYLSTRDATEMGELYSWLGLSVGINLAAK
                     SPLEKREAYNCDITYSTNSEIGFDYLRDNMVVRAEDMVQRPLNYALVDEVDSILIDEA
                     RTPLIVSGAQGSETNQLYFLADNLVKSLTTEDYIIDIPSKTIGLSDSGIDKAEKFFKL
                     DNLYDIENVAITHFLDNALRANYIMTYDIDYLVNEDQEVMIIDPFTGRTMEGRRYSDG
                     LHQAIEAKEGVPVQNESKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVV
                     PIPTNRPIARVDHEDLLYPSLEYKFNAVIADVKRRYEKGQPVLVGTVAVETSDLISQK
                     LVAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGPGVRELGGLC
                     VIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERIKVFMERMNLT
                     EEESVIKSKMLTRQVESAQKRVEGNNYDSRKQVLQYDDVMREQREIIYRQRQDVITAD
                     RDLAPEIKAMMKRTIERQVAGHFLGSKDEAIDGIIKFAHANLVEDDTLSKATFEAMNQ
                     KEIVEELYERALRVYDSQVKKLRDEERVREFQKVLILRVVDNKWTDHIDALDQLRNAV
                     SLRGYAQNNPIVEYQSEAFTMFNDMIGAIEFEVTRLMMKAQIHDNIERERTSQEAHTT
                     AVKNIMPNQSHAIQENVSFEGVDRNDPCPCQSGKKFKNCHGRK"
     gene            4551..4910
                     /locus_tag="U1099_001617"
     CDS             4551..4910
                     /locus_tag="U1099_001617"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEM4867929.1"
                     /translation="MIVGHGIDLQEMEAIESARIKHQGFPKKILTEKEFERYQSLSGR
                     RQLEYLAGRWSVKEALTKALGTGIGKIGFHDIEILNTSKGVPYVTKSPFDGNIWLSIS
                     HSGNFVQASVILEEKDD"
     gene            4903..6006
                     /locus_tag="U1099_001618"
     CDS             4903..6006
                     /locus_tag="U1099_001618"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEM4867930.1"
                     /translation="MIESEHRPTQIRVNLDAIAENFEQVMTNLPHKTEAFAVVKANAY
                     GHGAVAVAKKLSSQAAGFCVSNLDEALELRKAGIEHPILILGVVPVRFLPVAHQLNIS
                     VTVASLDWLQDAKDLEADLSGLTVHLKLDTGMGRIGFRKIADLLQAIAILEELEYDIE
                     GVYTHFATADEVDQAHFEYQLSIFKEFLDVLPITPRWIHASNSATSIWHADTVFNLVR
                     LGNILYGLNPSGRVLELPFDVQPALSLVSEIVHVKQVETGTTIGYGATYHSADSEWIA
                     TVPIGYADGLVRSLQGLSVLVDGQACEIVGRISMDQITIRLPHAYPLGQKVTLIGQDG
                     DKTISVQEWADCIGTINYEVVCLLTDRLPRIFE"
BASE COUNT         1999 a         1184 c         1458 g         1808 t
ORIGIN      
        1 ggagcctaag gcggagcgta actacatgat tattgatagt aattatgaga gttgggctat
       61 gaaacaaaaa acagcagact tactgttgtt caacaaagaa aagtccttgg ttggtttgga
      121 tcatattgca tatgttgagc caacttatat aacagaaaat aagaaaaatc atttgctaga
      181 catctataaa gctttgaaag gtaaggtaga agaagcgaaa gcaacggata cagatgataa
      241 agatgataaa caagaggggt ataatcgtgt tttacaattt gtcaattctg atattgataa
      301 attgagtact ctctccaaga ttacggaaga agaatttaaa gcactcgaaa attcaatgga
      361 tttagctcga gtgttcttgg ggcaaatgaa tgcgaaggct ggaaaagaat tttcggaagg
      421 agaaacttat caaagttatc taaagaatcg tcagaaaaat aacacaaatt ctgatgatga
      481 tagaaaccga aatgaattgc aagaaaatca agctaattct gatgaagtca gccaaaattc
      541 caaggatgct tcagctccaa gtgttaattc tgcacagcac tctgaggagc ttgaaggaac
      601 tccttcaaca caggaaacaa tctcagctgc tccttctcaa caaacaccag tagctcctaa
      661 ggcactacaa gctaaaactg agttagagga taaaacagag acttcatctg gttcctttga
      721 gagagaatcg aatgaagtgt cggctaatac agttttgtct cctgaaataa ctaagaacac
      781 gggaagtcta gcttctgtag aatctcgagt ggaggaggct gtggatagga caactccatc
      841 agaaaaagtt tctgctgaat cagaagaagt agaaaaaaca attgtatctg aggtatcatt
      901 ggatgcttca ttagcaaatg agaacaaaaa ttctgatgct caagaagata ctccacgttt
      961 ggagaaatct gccgaggtgg taacaactag tcaagaagta gctgaagcac cggtttctgc
     1021 tccacaagta agttctgaaa tcccaacttc accggaaact aattctacag aggcagtaac
     1081 aactagtcaa gaaatggctg aatcacgggt ttctgctcca caagtgagtt ttgtatcgcc
     1141 tactttagag gttcctaaaa atgagcgttt agacgagaaa gcagatgcaa ctatgccaaa
     1201 tggagttgga gcaaatactc atgaggcagg ttctgttccg acatcagata tttatgttca
     1261 agatgctggt tcagacacgg tgcctcaacc ttactcttta tctgcgacat tgtttgaaga
     1321 agcaatttca acagttgagc ctgtaggtgt tgcaacttct tctcaagaga gaagtgcagt
     1381 agctggcaag gtgaaagtgc ctgcctctct tgaacgtagt aacaattctg ttgaggagga
     1441 aaaagtagtt gattcgaatg caacaattga gaaacgagcg acagagaaaa aagaggtcct
     1501 tacttcggaa aatgtactta atagcttagt aacaatttgg gtagctttat ctactagttt
     1561 cttcatgaga tatttttctc gaggaaaata atatattaac acaggttgaa gataatattt
     1621 atctttgtgt agaaaattat tcatgtgagc ttttattaac cgaataatat gacaactgag
     1681 cttttaatga atttctaaat ctccgatata tcgcagattc ctcatttaaa attttataaa
     1741 tttttcacct cctaggagtt attgtgacta ggaggtgttt tgtggtaaaa atagtaatat
     1801 taagctagca ttttcactta tttaggctgt atcctagtgc caaaccttga aaattgccta
     1861 gttttctgct acaatatatt attgactaaa actgaaatag gatgaatgat atggtaacaa
     1921 atgtacttcg ttctctcatt gaaaatgata agggagagct aagaaaactt gagaaaatgg
     1981 cagacaaggt cttctcgtat gccgatgaaa tggaagcgct gactgatgaa cagttgcaag
     2041 ctaagacagc agaatttaag gagcgataca ataacggtga atccctcgac gatcttttgt
     2101 acgaagctta tgcggtagtc cgtgaaggag ctcgtcgcgt attgggtctc tatccctaca
     2161 aggttcaggt tatgggagga atcgttcttc acaacggtga cgttccggaa atgcgtacag
     2221 gtgaaggtaa aactctgact gcaacgatgc ctgtttattt gaatgccttg tctggtcaag
     2281 gtgttcacgt agtgacggtt aacgagtatc tttctactcg tgatgcgact gaaatgggtg
     2341 aattgtattc gtggcttggt ctatcagttg gtatcaactt agctgctaaa tcacccttgg
     2401 aaaaacgtga ggcctataac tgcgatatta cttattcgac caactcagag attggttttg
     2461 actatctccg agacaatatg gttgttcgcg cagaagacat ggttcaacgt cctctcaatt
     2521 atgccttggt cgatgaagtt gactcaatct tgattgacga agctcgtacg ccgttgattg
     2581 tatctggtgc acaaggttcg gaaacaaacc agttgtattt cttggcagat aatcttgtta
     2641 aatctttgac aacagaagat tacatcattg acattccatc taaaacgatt ggtctgtctg
     2701 attcaggtat tgacaaagca gagaaattct tcaagttaga taacctctac gatattgaaa
     2761 atgtagccat tacccatttc ttggataatg cacttcgtgc caactacatc atgacctatg
     2821 atattgacta tctggtcaat gaagaccaag aggtcatgat tatcgatcca tttacaggtc
     2881 gtacgatgga aggtcgtcgt tattctgatg gacttcacca agccatcgaa gctaaggaag
     2941 gtgtaccggt tcaaaacgag tctaagacaa gtgcctctat tacctaccaa aaccttttcc
     3001 gtatgtataa aaaattatca gggatgacgg gtactggtaa aacagaagag gaagaattcc
     3061 gtgaaatcta caacatccgt gtcgtaccaa tcccaaccaa ccgcccaatc gcgcgtgttg
     3121 accacgaaga tctactctat ccaagtctag aatacaaatt taatgccgtt attgctgatg
     3181 tgaagagacg ctatgagaaa gggcaacctg tcttggtcgg tacagttgcg gttgaaactt
     3241 ctgatttgat ttctcaaaaa ttggtagctg caggtgtccc tcacgaagta ttaaatgcaa
     3301 aaaaccacta tcgtgaagcg cagattatta tgaatgctgg tcagcgtggt gcggtcacta
     3361 ttgcgaccaa catggcggga cgcggtaccg atattaagtt aggtccaggt gtacgtgaac
     3421 ttggtgggct gtgtgttatt ggtactgagc gccatgaaag tcgtcgtatt gataaccagc
     3481 ttcgtggacg ttcaggtcgt caaggtgacc caggtgaatc acaattctat ctatctctcg
     3541 aagatgactt gatgaaacgc tttggttcag aacgcatcaa agtatttatg gagcgtatga
     3601 atctaacaga agaagagtcc gtcatcaagt ctaaaatgtt gacacgtcaa gttgaatctg
     3661 ctcagaagcg tgtggaaggg aacaactacg actcacgtaa acaagtcctt caatatgacg
     3721 atgtgatgcg tgagcaacgt gaaatcatct atcgccaacg ccaggatgtt attactgctg
     3781 accgtgattt ggctcctgaa atcaaagcga tgatgaaacg gacgattgaa cgccaagttg
     3841 cgggtcactt ccttggttcg aaagatgaag ctattgatgg aattatcaaa tttgcgcatg
     3901 caaatctcgt tgaagatgat accttgagta aagctacttt tgaagcaatg aatcagaaag
     3961 agattgtcga agagctctat gaacgtgctt tgagagtcta tgattcgcaa gtgaaaaaat
     4021 tacgcgatga agaacgtgta cgcgaattcc agaaagttct tatccttcgt gttgtggata
     4081 acaaatggac agaccatatt gatgccttgg atcaattgcg caatgcagtc agtcttcgtg
     4141 gatacgctca aaacaatcca atcgttgaat atcaatcaga agcatttact atgttcaacg
     4201 atatgattgg ggctatcgag tttgaagtaa cccgcttgat gatgaaggcg caaattcatg
     4261 acaatattga gcgtgagcgt acaagtcaag aagcacatac gacagctgtt aagaatatta
     4321 tgccgaacca atcgcatgca attcaggaga atgtatcttt tgaaggtgtt gatcgtaacg
     4381 atccatgccc atgccagtct ggtaagaaat ttaaaaattg tcacggccgt aaataggaat
     4441 gacgcatgga ctggacggct gtccagtcct tttgcaattt ttagacaagt tactagagtc
     4501 agttcgttta ttttaataaa tgaaatgact atgcaaaagg agaccgatta atgatagtag
     4561 gacatggcat tgaccttcag gagatggagg cgattgaaag tgccagaatc aaacaccaag
     4621 ggtttcccaa aaagattctc acagagaagg aatttgaacg gtatcaaagt ctgtccggtc
     4681 gccgtcagct agaatattta gctggacgtt ggtcagtcaa agaagcgctg accaaggcct
     4741 tggggacagg tattggtaag ataggatttc atgatattga aattctgaat accagcaaag
     4801 gagttcccta tgtgacgaag agtccctttg atgggaatat ttggctcagc attagccatt
     4861 caggaaattt tgtacaagct agtgtgattt tggaggagaa agatgattga aagtgaacat
     4921 cgacccacgc aaattcgggt taacttagat gcgattgcgg aaaatttcga gcaagtaatg
     4981 accaacttgc cccataagac cgaagcattt gcagttgtta aggcgaatgc ttatggtcat
     5041 ggagctgttg cggtagcgaa aaagttatcc agtcaagcag ctggtttttg tgtttcgaat
     5101 ttggatgaag cattagagtt acgtaaagcg ggtatcgagc atcctatctt gattttagga
     5161 gtggttcctg tacgtttttt accagtagct catcaactca atatcagtgt gacagttgct
     5221 agtctggact ggttgcaaga tgcaaaagac ttagaggctg atttatctgg tctaactgtc
     5281 catcttaagc tagatacagg tatggggcgg attggtttta gaaaaatagc cgatttgcta
     5341 caagcgattg ctatccttga agaattagag tatgacatcg aaggagttta tactcatttt
     5401 gctactgcgg atgaggttga tcaggcgcac ttcgagtatc aactgtctat ttttaaagaa
     5461 tttttggatg ttctaccaat tactccgcgc tggattcatg ccagcaattc agcaacaagc
     5521 atttggcatg ctgatacagt atttaacctg gttcgtttgg gaaacattct ttatggactt
     5581 aatccaagtg gacgagtact agaactcccg tttgatgttc aaccagcctt atctctcgta
     5641 tctgaaattg ttcatgtaaa gcaggttgaa actggcacaa caattggcta cggagctact
     5701 tatcacagcg ccgattcaga atggattgcg accgttccga taggatatgc agatggtctc
     5761 gtacgaagct tacaaggctt atcagtccta gtcgatgggc aggcctgtga aattgtaggg
     5821 cgtatttcga tggaccagat caccattcgc ttacctcatg cttatccact tggtcaaaaa
     5881 gtcactctaa tcggtcaaga tggagataaa acgatctctg ttcaggagtg ggctgattgc
     5941 atcggaacca taaattacga ggtggtttgt cttttgacag atcgcctacc tcgaattttt
     6001 gagtaaacgt gacgataatc cgcctctttc actaggatag aaggctaaaa tattagagag
     6061 gtattgccta gagattgtct ataatacaaa tagcgtcatt tagttgactt ttattacttc
     6121 gacaatcgag gaaatatcgt ttccatattt ccaacttcaa acactccact ggattgtttg
     6181 aactcgcctt gcctgatttt gtaaaagtga cttgctttgc aagcaatctg ggggatacga
     6241 atagtccagc ggactattcg tacctgagcc tgaaaacaaa aaagcgaagt gcatcagctg
     6301 gtcacctaga aataagaaag cattgagtta tgcctgcggc ttttgatgtg aacatcgttc
     6361 actttgtccc cagtctccaa ctatctccca tatagttgga gcgagtgcta aaagtaaact
     6421 aaaagactat aatgagtctg gtcaaaaag
//
LOCUS       DASFSW010000106         6360 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.106, whole genome shotgun sequence.
ACCESSION   DASFSW010000106 DASFSW010000000
VERSION     DASFSW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.106"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..177
                     /locus_tag="U1099_001619"
     CDS             <1..177
                     /locus_tag="U1099_001619"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM4867931.1"
                     /translation="TAIEYSLKYETTFRTVLSNSNLVLSNNMAERSIKTLVIGRKNWI
                     FSQSFEGASLQLSF"
     gene            174..332
                     /locus_tag="U1099_001620"
     CDS             174..332
                     /locus_tag="U1099_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEM4867932.1"
                     /translation="MSLLETAKRHGLDAEKYMTYLLEHLPNEETLAKKEVLEAYLPWD
                     KNIKRACK"
     gene            546..770
                     /locus_tag="U1099_001621"
     CDS             546..770
                     /locus_tag="U1099_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867933.1"
                     /translation="MYQRLRDLREDNDYTQKDVANHLTLTHSAYAKIERGDRQLSVEV
                     LIKLSSLYAVSTDYLLGLSDYPKRIIHYKK"
     gene            1042..1182
                     /locus_tag="U1099_001622"
     CDS             1042..1182
                     /locus_tag="U1099_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867934.1"
                     /translation="MEKKQTGADLPQVVIEGYKVMVQVTNKSLSLTDILDDLLGRKLE
                     KI"
     gene            1281..2855
                     /locus_tag="U1099_001623"
     CDS             1281..2855
                     /locus_tag="U1099_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM4867935.1"
                     /translation="MTKTCIYLRLSRDDGDNVESNSIINQRSLLRDYARNHDFTILEE
                     FVDDGISGLNFNRPDFNRMMAMVNDKTIDTIIVKDLSRFGRDYIETGKYLQRIFPAMG
                     VRFISVNDHYDSLTADTNETHLVMPIKSLINDSYCRDISTKVRSTQKAKRQKGEYIGA
                     FAPYGYRKDYKQRNHLVVDETIRPTIEKIFALKLEGYSSNAIAGFLNQAGIETPLQRK
                     KACQEKIKGFYGHSYKWDTKMVNRILTNRVYTGSLEQGKQTKLNYKSQKSVAVNPSDW
                     VRVDDTHEAIISKSTFEIANNLLLRDVKHGKKLPDLFAGLLFCSDCQAQLVQRKLRYK
                     DKETVQYICSTYNNGRGCSRHAIKQERLLDLVSTFIHQHLDWKSYLIKAVPDYVNQEQ
                     FLEVDFTSLLENRKKYEQLLQSLYLDLDDGLINDTEYQEFQRSYRQKLTQIEDTILQK
                     KLLAQTLYNKLRDKKKWLDQLSMMQTDQVLNRLTLVTLLDRIVIHENQELTLVFHDMP
                     ELEVLQQFSHKETEVV"
     gene            2855..4471
                     /locus_tag="U1099_001624"
     CDS             2855..4471
                     /locus_tag="U1099_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM4867936.1"
                     /translation="MVRTKNRYRMSTVEEIPCQTYRTGIYIRLSKERTESWRNKSQSL
                     ETQESLARAFAKKKGLVVTKCYIDYEYSGTTFHRPAYQDMMDDIKKGVINCIIIRDLS
                     RLGRNYIEMGRLIDKVFPFMGVRFISINDNLDTLNGLEDKKSFEVEIKNLVNDLYSKD
                     ISKKVTASHIQKAKQGYFIAGFAPYGYKLERREGGNRLVIDAIVQPVMVDVFEKLIAG
                     QSINQVTKYLNQQKVATPMAYRQTGERYRTENDTRQWQSANLQRLVQNPAFYGSLIQR
                     RHNKTLTEKDFIQVDDCHEGYITKEEYQTLQKLIRERRSIRKQTQPRTTNRYKGLIYV
                     KGQSTQMRRYCKHFSNPKSEYDYYYFMDYLHGSAPSEKQTVYISEQALDKIVLSLVQT
                     EMSRLGTVKNLLSKLEMKRNECLRDCQMKIKKFQRTLASLQAEQSELYLNYRLEAQER
                     GTYLSQKATIISKIETLSTEISHYEALAVKIEEKHSQQVSWVKHLAKCQNQSVLTAEL
                     LNEVIERIDVEVDKSVTVTFTCLMGGDDHV"
     gene            4464..5900
                     /locus_tag="U1099_001625"
     CDS             4464..5900
                     /locus_tag="U1099_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM4867937.1"
                     /translation="MSKMALYLRLSVDEDGTDESNSITNQRYLLNQYLDQSSDFNHWE
                     RLEFVDDGFSGTSLNRPAFQRLMADVKAGIIRHIIVKDLSRFMRDYLELGNYLENIFP
                     FLGVRFIAINDQYDSLTSEQNGLDIDVPFRNLLNDFYAKDVSEKVKSAMNSMKRSGKN
                     MSWLPPFGYIKDPEDRFKIIIDEAVAPIVRRIFTLYLDGNSYQKIARILNKEHVITPA
                     ERKMQISQARYAKSLRLTTEQKRNVWSSTTVGQILKNEAYKGTYLFNTRTYVRGKHVF
                     RPKSEWERIENNHEAIISSELFEQVARLKAKKVIQKKHSHPPKIPSILTELIRCEHCD
                     HSLIGSYNHQGYQYFSCRYCKGQGEKMKSCRADKIEQAIKEKLGYEEDKSRPKASKTR
                     VLREIERLKRQKLSFFQDYKDSLMTREDYITKKMVIDKTVRNLEEQLKEVNTFQLLSN
                     DRLTKEIVSTHIEKVVVDCLGFFTVIYK"
     gene            6010..6354
                     /locus_tag="U1099_001626"
                     /pseudo
     CDS             6010..6354
                     /locus_tag="U1099_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
BASE COUNT         2101 a         1116 c         1369 g         1774 t
ORIGIN      
        1 actgcaatag agtatagcct caaatacgaa accaccttcc gaaccgttct ctcgaacagt
       61 aacttagtcc tgtccaataa tatggctgag agatcgataa aaacgctagt aattggccga
      121 aaaaattgga ttttttctca gagctttgag ggcgccagtc tacagctgtc attctgagtc
      181 ttttagaaac tgctaagcga catggactag atgcagagaa atatatgact tatcttctag
      241 aacacttacc taacgaggag acgctcgcaa aaaaggaggt gctagaagcc tatttgccat
      301 gggataaaaa tattaagaga gcatgtaaat agaaaaaggt tccagagtcg acaggacttt
      361 ggagcctttt tcaatatacc ttgttattgg gcggttactc tcttccatgt tgtttgtagc
      421 tgtttttctc gtcacttaca tgatgagcat aattaaaatt tacattgaat tttaaattgt
      481 ccagtttgga caatttaaaa gataacaacg agatatactt tatactgggt ataggagaaa
      541 gctagatgta ccaacggcta agggatttga gagaagataa tgattatact caaaaagatg
      601 tagctaatca tctcacttta actcactcag catatgctaa gattgaaaga ggagatcgtc
      661 aattgtctgt agaagtattg ataaaacttt ctagcttata tgccgtaagt acagattatt
      721 tgcttggttt gtcagattat cccaagcgaa taatacatta taaaaaatag tctatcctat
      781 ctgccctttg acaactgaat agactgaagt gggactttaa tcatttgtgg agcaagttag
      841 tcagacacgc tgtgatacga gcgagaatag tcagttataa aataggttgg ttacctatct
      901 gccatgatgc aagtgaggat aatgatactt ctaaccgttt aggctgccag cgagaaagga
      961 cagcgggtgc aacccccatg tggaacttaa ccagttctgc ccactgatgt cataaatttt
     1021 aaagtaaagg aaataagatt tatggaaaaa aaacaaacag gtgcggattt accgcaggtt
     1081 gtgattgaag gttataaggt gatggtgcaa gtgacgaata agtcacttag tttgacagat
     1141 attttagatg atttattagg tcgaaagctc gaaaaaattt gagtttttag cggatatatt
     1201 tttagaaatg atgtaaaata gagttgtagg acctcaatcc ttaatcggta agcacctaaa
     1261 tactgattaa ggagatacac atgacaaaaa cctgtattta tttacgctta tcacgagatg
     1321 atggggataa tgtggaaagc aattccatca tcaaccaacg ctcacttttg cgagactatg
     1381 ccagaaatca tgatttcacc attttagaag aatttgtaga tgatggcatt agcggcttaa
     1441 actttaaccg ccctgacttt aaccgaatga tggcaatggt taatgataag actattgata
     1501 cgattatcgt caaggacttg tcacgcttcg gtcgtgacta tattgagact gggaaatatc
     1561 ttcagcgaat ttttcctgct atgggagtca gatttatctc ggtcaatgac cactatgata
     1621 gcctgacggc tgataccaat gaaacacact tggtcatgcc cattaaaagt ctaattaacg
     1681 atagctactg tcgagacatt tcaaccaaag tccgaagtac ccaaaaagcc aaacgtcaaa
     1741 aaggcgagta tattggagct tttgcccctt atggctatcg gaaagattac aagcaacgga
     1801 atcatttagt ggttgatgag actatacgcc caactatcga gaaaatcttt gctctgaaac
     1861 tagagggata ttcctcaaac gctattgcag ggtttcttaa tcaagctggg atagaaacgc
     1921 ctctacaacg taaaaaagcc tgtcaggaaa agattaaagg cttttatggg catagctata
     1981 agtgggacac gaaaatggtc aatcgtattt tgacaaatcg tgtctatact ggtagtttgg
     2041 aacaggggaa gcaaaccaaa ctcaattaca agtcacagaa atctgtggct gtcaatccat
     2101 cagattgggt acgagttgac gatacgcatg aggcgattat ctcaaaaagt acgtttgaga
     2161 tagccaataa tctcttactc agggatgtca agcacgggaa gaagttgcct gacctctttg
     2221 caggattatt attttgcagc gactgtcagg ctcaacttgt acaacgaaaa ctccgctaca
     2281 aggacaaaga gactgttcaa tatatttgta gcacctacaa taatggacga ggatgtagtc
     2341 gccatgcaat taaacaagag agattactag acttagtatc gacctttatt catcaacatc
     2401 ttgactggaa gtcttatctg attaaagcag ttcctgatta tgttaatcaa gaacagtttt
     2461 tggaagttga ttttacttct ctacttgaga atcgcaagaa gtatgaacag ttgctacagt
     2521 ccttgtactt agatttagac gacggattga ttaatgatac agaataccaa gagtttcagc
     2581 gaagctatcg tcaaaaacta acacagatag aggacaccat tcttcaaaag aaattactgg
     2641 ctcagacctt gtataacaag cttcgagata agaagaaatg gctagaccag ttgagtatga
     2701 tgcaaactga ccaagtgctt aatcgtctca ctctagtcac cttacttgac cgtattgtca
     2761 ttcatgagaa ccaggagttg accctggtgt ttcatgatat gccagaacta gaagtcctcc
     2821 agcagttttc tcataaagag acggaggtgg tctaatggta cgtaccaaga accgttaccg
     2881 tatgtcaacg gtggaagaga taccatgcca aacttatcgc acaggaatct acattcgtct
     2941 gtccaaagag cgtacggaaa gctggcgaaa caaatcgcaa tccttggaga cacaagaaag
     3001 cctagctcga gcttttgcta agaagaaagg gttagttgta accaagtgct acattgatta
     3061 tgaatattct gggacaacct ttcaccgacc ggcctatcag gacatgatgg atgacatcaa
     3121 aaaaggtgtc ataaactgta ttatcattcg agacttatcc cgtttgggtc gtaactacat
     3181 tgagatggga cgactgattg ataaggtgtt tccctttatg ggggttcggt ttatttcaat
     3241 caatgacaat ttagacacac tcaacggatt agaagataag aaatcctttg aagtggaaat
     3301 caagaatttg gtcaacgact tatattctaa ggatatttca aagaaagtta ccgctagtca
     3361 tattcagaaa gcaaagcaag gttatttcat cgctggcttt gctccctatg gatataagtt
     3421 agaaagaaga gagggtggta atcgtttagt cattgatgcg atagttcaac ctgttatggt
     3481 agatgtcttt gaaaagttaa tagcaggcca atctatcaac caggtgacaa aataccttaa
     3541 tcagcagaaa gttgctacgc caatggccta tcgccaaaca ggagaaaggt atagaacaga
     3601 gaatgataca cgacaatggc aaagtgcgaa ccttcagcga cttgttcaaa atcctgcgtt
     3661 ttatggtagc ctcattcaac gtcggcataa taaaacctta actgagaaag atttcattca
     3721 agtagatgat tgtcatgagg gttatattac aaaagaagag tatcaaaccc ttcaaaaact
     3781 aattagggaa cgtcgttcca taagaaaaca gactcagcct cgtactacta atcgttataa
     3841 gggtcttatc tatgtcaagg ggcaatcaac tcaaatgcga aggtactgta agcatttctc
     3901 aaatcccaaa tcagagtatg attactatta ctttatggac tatcttcatg gttcagcacc
     3961 ttcagagaaa caaactgtct atatcagtga acaagcctta gataagatag tgttgagctt
     4021 ggttcagaca gagatgagtc ggcttggcac ggttaaaaac ttattgtcca aattagaaat
     4081 gaagaggaat gaatgtctta gagattgcca aatgaaaata aaaaaatttc aaaggacttt
     4141 ggctagtcta caagctgagc aaagcgagct ttatctgaat taccgccttg aggctcagga
     4201 acgagggact tatctgtctc aaaaggcgac gattatctca aaaattgaaa ccctctcaac
     4261 ggaaatatca cattatgagg ctttagcggt gaaaattgag gagaaacaca gtcagcaggt
     4321 cagttgggtt aaacatcttg ctaaatgcca aaatcaatct gttttaacgg cagaactgct
     4381 taatgaagtg atagaacgga tagatgttga agtggataag tctgtgacag taacttttac
     4441 ctgcctgatg ggaggtgatg accatgtcta aaatggcttt ataccttcgt ctttccgtcg
     4501 atgaggacgg gacagacgaa agcaactcca ttaccaacca acgctatctt ttgaatcagt
     4561 accttgatca gtcatcagac tttaatcatt gggaacgctt ggaatttgta gatgatggtt
     4621 tttcaggaac aagtctcaat cgacctgcct tccaacgctt gatggctgat gttaaggcgg
     4681 gaattatccg tcatatcatt gttaaagatt tgtctcgttt tatgcgtgat tatctggaat
     4741 tagggaatta tttggaaaat attttccctt ttctcggagt tcgcttcata gctataaatg
     4801 accaatatga tagtttgaca tctgaacaga atggactgga tattgatgtt ccgtttagaa
     4861 accttctcaa tgatttctat gccaaggatg tttctgaaaa ggttaagagt gcgatgaaca
     4921 gcatgaaacg ctctggaaaa aacatgagtt ggttgccacc atttggctac atcaaggatc
     4981 cagaagaccg attcaagatt atcattgatg aggcagttgc accaattgtt agacgtattt
     5041 ttaccttgta ccttgatggt aatagttatc aaaagattgc tcgcatcttg aataaagaac
     5101 acgtcattac cccagcagag cgaaaaatgc aaatcagtca ggcacgttac gcaaagtcac
     5161 tccgtttaac aactgagcaa aaacgaaatg tttggagtag cactaccgtt ggtcaaattt
     5221 taaagaatga agcctataag ggaacttatc tgtttaatac tcggacgtat gtaaggggga
     5281 agcatgtttt cagaccaaaa tcagagtggg agcggattga gaacaatcac gaagcgatta
     5341 tttcatcaga actatttgaa caagttgctc gattaaaagc taagaaggtt atccagaaaa
     5401 aacactctca tcctccgaaa attcctagca ttttaacaga actgattcgt tgtgagcatt
     5461 gtgaccattc tttgattggt tcatataacc atcaaggtta tcagtatttt tcctgtcgct
     5521 attgtaaagg acagggagaa aaaatgaagt catgccgagc agacaagatt gagcaagcaa
     5581 tcaaggagaa acttgggtat gaggaggata agtcccgccc aaaagcttct aagactagag
     5641 ttcttagaga gattgagcga ttaaagagac aaaaactatc ctttttccaa gactacaagg
     5701 atagcctgat gactagggaa gattacatta ctaagaaaat ggtaatagac aaaactgtta
     5761 ggaatttaga agaacagctg aaagaagtta atacatttca gttgttgagt aatgataggt
     5821 taacaaaaga aatagttagc actcacattg aaaaagtagt tgttgactgc ctcggatttt
     5881 ttacagttat ctataaatag ttgaggaaga gacttgaatc ctatcaagtc tctttcttga
     5941 tgggatagtt tgtggtataa tgagtgtgaa atctagtaaa taaatagtgt cagtagcttg
     6001 acataagagg gacatattga agaaggcgag aatttccatg attcagttgt tagagaagtg
     6061 caagaagaaa caggtttaac agtcactgat gctcgcttgg ttggtcttaa acattggccg
     6121 gataaggaag ggcatcgtta cattgtgttt ctctataagg caacagagtt tacaggaact
     6181 atccgatcaa ctgaagaagg tgaggtacgt tgggtcgaga agtcggacct acctcagatg
     6241 gatttggcct atgatttgct agagatactg aaagtcatgg atgagccaga tttgtccgag
     6301 ttcttttata ctagaaaaaa tgacgatgga gagtgggaca agaattttag gtagaagcta
//
LOCUS       DASFSW010000107         6310 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.107, whole genome shotgun sequence.
ACCESSION   DASFSW010000107 DASFSW010000000
VERSION     DASFSW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.107"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            787..2697
                     /gene="ltrA"
                     /locus_tag="U1099_001627"
     CDS             787..2697
                     /gene="ltrA"
                     /locus_tag="U1099_001627"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM4867938.1"
                     /translation="MAKRIVNKAERNAERYDAKEIGYQLYEDSLNGKRFDRLMPMIVS
                     DQNIILAYRNICKNNGSKTPGTDGKTIVEIQKLPIEMVIKTIRNKLNYYQPKKVRRVE
                     IPKDNGKTRPLGIPSIWDRLIQQCVLQILEPICEAKFHERNNGFRPYRSTQNAIAQCY
                     KMAQIQNLHFVVDVDITGFFDNIDHSKLIRQLWGLGVQDRKLIIIIKQMLKADILFND
                     IVITSETGTPQGGILSPLLANVVLNELDWWVANQWEMFKIKEGSTGYEFTKVDNEGNI
                     LTIDRTQKWNKLRAKTRLKEMYITRYADDFKIFCRDYATAVKVMKATNLWLAENLHLR
                     TSDEKSGITNLRKNYTTFLGIKFKVVPKGNKWIIRSHMADKSKVKVISKLGSVWKDIK
                     NPSKQSELDKNISLYNAMVMGMHNYYCMATLVSSDFAEIAFKVTGKSNGMNHNKRCFP
                     IEKQGEITSKYILDKYGKSKQCRWINGRMIVPVGYVSYEYPKYKRREVNKYVRKYSDA
                     ENCISFEVMKYMMENAHLYPTLEMADNALSRYIAQKGKCAVTHNALTIVDMVCEHIKP
                     CKGERNDTYRNLIILSKEVSDLVGEENSNKVSKLLKNLPLTKEMQDKINKLREYRELE
                     MIQFEDYIGTKK"
     gene            2720..3340
                     /locus_tag="U1099_001628"
     CDS             2720..3340
                     /locus_tag="U1099_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEM4867939.1"
                     /translation="MMGNYHARCGAGEKPEVVTPEAYLSLFGEIPDFAEQTSTVRSRN
                     MSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQTIDV
                     RSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPPKH
                     KNWKWLADKETDERWWHYRINPLTAEEELELSGHKIRDLSTETTLH"
     gene            3358..3600
                     /locus_tag="U1099_001629"
     CDS             3358..3600
                     /locus_tag="U1099_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867940.1"
                     /translation="MNQKLSGFVYGVDASSMFSQAMSLLQKGLIAVGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGTFVTQITI"
     gene            3619..4473
                     /locus_tag="U1099_001630"
     CDS             3619..4473
                     /locus_tag="U1099_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEM4867941.1"
                     /translation="MIMNFTSSFVYLASEKVSSDSLFEGFQVDLESTANLVKSLADFN
                     PTVWSYMTAITKGIMQPLGVAILAVVLVLEFSKMAKKIANSGGAMTFEAMAPMIVSYI
                     MVAVVITNTTVIVEAIIAIASYVIEQVAELVTNGGANYDTISGIKGSGIVGKMIIGFF
                     AILIWLVRMASIMVVNILITIRFIQLYLMIPFAPVTIPTFLSDDWRSVGIGYLKNIMV
                     YAVQGILIFLIVSLVPLFESAGKIAVSNGAGVMESLAIMFGGLVQAILLIIALVGSQR
                     TARSILGM"
     gene            4535..4888
                     /locus_tag="U1099_001631"
     CDS             4535..4888
                     /locus_tag="U1099_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEM4867942.1"
                     /translation="MNTRVFKDISKVQHRAWLGFTTRQVIFVFPAVALTILVLGLNLF
                     YWQFGDWFVYGFVFSFTIPLMLFGVYRPNDLPFETYLKYRFHYELTVPDRTFTGRKGD
                     QREKIKTLNETKDLF"
     gene            4845..>6310
                     /locus_tag="U1099_001632"
     CDS             4845..>6310
                     /locus_tag="U1099_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HEM4867943.1"
                     /translation="MKKLKHLMKPKTSSNDKKQKTKTQKQEIRPSTVNTLAYQGLFQN
                     GLMQVSPSYFSQTYLLGDVNYQTVGLDDKGAIVEKYSDLINSLDDKTNFQLTILNQKV
                     NLEKFRKSILYPLQEDGFDAYRDELNRMMNANLEAGENNFSAVKFLSFGKSDQTPKLA
                     FRSLSQIGEYFKSGFSEIDVSLGLLGGEERVNVLADMLRGENHLPFSYKDLTLSGQST
                     KHFIAPTYLSFKHKNHIELDDRLLQIVYVRDYGMELGDKFIRDLMQSDLEVMISLHAK
                     GSTKSETMTKLRTKKTLMESQKIGEQQKMARTGIYLEKVGHVLENNIDEAEALLQTMT
                     QTGDKLFDTVFLIGVLADTEDQLKQSLDIIKQVAGSNDMIIDNLTYMQEAAFNSLLPF
                     GKNYLEGISRSLLTSNIAVNAPWTSVDIQDKGGKFYGINQISSNIISIDRGKLNTPSG
                     LILGTSGAGKGMATKHEIISTKLKEADSDTEIIIVDPE"
BASE COUNT         2053 a         1121 c         1399 g         1737 t
ORIGIN      
        1 aagcggtagg aataaccgac aaaagcgtag cctaaagtca caaataattt aataatgtgg
       61 ttttagagaa ctgcataact gaaatttcaa atgaaggggt aacgcccaga agggaattcg
      121 ctaatactcc gaatagcgtt agttgaatac agatgactaa ggttataagc tcggagaagt
      181 cgtgcgaagc acaccctaaa gtagaaatac taggaaagat acctagaggt aggtgttgtg
      241 tgtgacagtg cgggcggggt tgccatatgg ttagaatgac caaataggcg gtctgacaaa
      301 attccgaatg tacggctcta aacgaatggc tggtagaaat gccagtgtac caatgggtac
      361 gcaagtgggg taaagtaaaa gttgttagta tgaaataccc tctgtgttta caggacacag
      421 gtatcttgag aaagctcttg aagataatac ttgcagggct aaaggaatga cctaagtggt
      481 aacaatccta agttatgtgg aacgtggaaa gcaaccaata cgggagctga ctgtatcagc
      541 gacaaagtac cgtagttgta tcgaaaatga aaaggctgaa agaacctttt ctacaataag
      601 tgcatttatg gttgtgagag cagtggcaca gtaccgttga agccgtgata ataagcggtg
      661 gagggatagc cacaagccga tactaaattt tctttcatgt ctatatagta atcaccaaaa
      721 ggagattttc atagctgtcg tggtgcgact aagaaaaaat cattctccga taaggagttg
      781 gttactatgg caaaacgaat tgtaaataaa gcagaacgaa atgcagaaag gtatgacgct
      841 aaagaaatag gctatcagct ttatgaagat agcctaaacg gtaagagatt tgataggctt
      901 atgccaatga ttgtctctga ccaaaacatc atacttgcat atcgaaatat ctgcaagaat
      961 aacgggagta aaactcctgg tacagatggt aaaacaattg tagaaataca gaaattacct
     1021 atcgaaatgg tcataaaaac catcagaaac aagttgaact attatcaacc aaagaaagtc
     1081 agacgagtag aaatcccgaa agataatgga aaaacaagac ctcttggtat tccgagcatt
     1141 tgggacagac taatacaaca gtgtgtctta cagatactag agccaatctg tgaagctaag
     1201 tttcacgaaa gaaataatgg tttcagacca tataggtcta cgcaaaacgc aatagcacag
     1261 tgctataaaa tggcacagat acaaaatctg cactttgttg tagatgtaga cattacaggg
     1321 ttctttgata atattgacca ctcaaaactt atccgtcagt tatggggatt gggagtacag
     1381 gacagaaagc tgattataat aatcaagcaa atgttaaaag cggacattct gtttaatgac
     1441 atagttatta catcagaaac aggcacacct caaggtggta ttctatctcc gttactagct
     1501 aatgtagtgt tgaatgaact ggactggtgg gttgctaatc agtgggaaat gttcaaaatc
     1561 aaagagggaa gtacaggcta cgagtttaca aaggttgata atgaaggaaa catactcaca
     1621 atagaccgaa cgcagaaatg gaacaagctc agagcaaaaa caagattgaa agaaatgtat
     1681 attactaggt atgcagatga cttcaagatt ttttgcagag attatgcgac agctgtcaag
     1741 gtaatgaagg ctacgaatct atggttggct gaaaatctgc atttgcggac aagtgatgaa
     1801 aaatcaggaa ttaccaacct tagaaagaac tacacgactt ttcttggaat aaaattcaaa
     1861 gtagtaccaa aagggaataa atggattatt cgttcccata tggcagataa gagtaaagtc
     1921 aaggttatca gtaaactggg ttctgtttgg aaagatatta aaaacccaag caaacaaagt
     1981 gaattagata aaaatataag tctctacaac gctatggtca tgggaatgca caattattac
     2041 tgtatggcaa cgcttgtgtc atccgacttt gcggaaatag cctttaaggt tacagggaaa
     2101 agcaacggaa tgaatcataa taaacgatgc tttcccatag agaaacaagg tgaaattaca
     2161 agcaaataca tcttggataa gtacggaaag tccaaacaat gtagatggat aaatggtcgt
     2221 atgattgttc ctgtcggata tgtatcttat gaatatccaa aatataaaag acgtgaagtc
     2281 aacaaatatg tgaggaaata ctcagacgct gaaaactgta tcagctttga agttatgaag
     2341 tatatgatgg aaaatgctca tctctatcct acattagaga tggcagataa tgctctttca
     2401 aggtatatag cacaaaaagg caaatgtgct gttacgcata atgctttgac catcgtggat
     2461 atggtctgtg aacacatcaa gccttgcaaa ggagagcgta acgataccta caggaattta
     2521 attattctct caaaagaagt atcggatttg gtaggagaag aaaatagcaa taaagtaagt
     2581 aagctactca aaaacctacc attgaccaaa gaaatgcaag ataaaatcaa taagcttcgt
     2641 gaatataggg agctagaaat gatacaattc gaggactata taggcactaa aaagtaagat
     2701 ttagtcggtg gaacgccgtg tgatgggaaa ctatcacgca cggtgtggag cgggggaaaa
     2761 gcccgaggtg gtaacaccag aggcttacct atcgctattc ggagaaatcc cagactttgc
     2821 tgaacaaacc tcaacagttc gctctaggaa catgagtcta gttccaatct tacaaaatat
     2881 cgcccaactc caaggacttt ataaggaaaa agaggcttgg aaaactattc tgggaaactg
     2941 tgatagcctc ctctatttgg gtggaaatga cgaggaaact ttcaaattta tgagtggtct
     3001 attaggcaaa caaaccattg atgtcagaag taccagtcgt tcttttgggc agactggctc
     3061 aagttctacc tctcaccaga aaattgcccg tgacttgatg acggctgatg aagtcgggaa
     3121 tatgaaacga gatgagtgcc ttgtacgcat tgcaggggtt cctgtttttc gaaccaagaa
     3181 atattttcca cccaaacata agaattggaa atggcttgcg gataaggaaa ccgatgaacg
     3241 ctggtggcac tatcgcatca atcccctaac cgctgaggaa gagctagaat tgtcaggcca
     3301 taaaataagg gatttaagca cagaaacgac actacattaa tagaaatgag gaattatatg
     3361 aatcaaaaac tatcaggctt tgtttacgga gtggacgcta gctccatgtt ctcccaagcc
     3421 atgtctctat tacaaaaagg tttgattgca gtaggagcct ttcttgttgt catgggcatt
     3481 atcaaccttt caaccaacat taaagatggt gggccaggtg tccgaaatgc cattctagaa
     3541 attgttggtg gggtcatggt gggagccgcg ggtacttttg tgactcagat taccatttag
     3601 gggaggacag cacatgacat gataatgaat tttacgtcat cttttgtata tctagcctct
     3661 gaaaaagtgt ctagcgatag cctttttgaa gggtttcaag tggatttaga atcaaccgcc
     3721 aacttggtta agtcactagc ggactttaat ccgacggttt ggtcttacat gacagccatt
     3781 accaagggga ttatgcaacc cttgggagta gctattcttg cggttgttct cgtactcgaa
     3841 ttttcaaaaa tggccaagaa aatcgcaaac tcaggtggtg caatgacctt tgaagctatg
     3901 gcacctatga ttgtcagcta catcatggtc gcggtcgtga ttaccaatac gacggttatt
     3961 gtggaagcca ttattgccat tgcctcttat gttattgaac aagtggcaga gcttgtcacg
     4021 aatggtggtg ctaattatga taccatctca ggcattaagg gatctggaat tgtaggtaaa
     4081 atgattattg gcttttttgc gattctcatt tggttggtac gaatggccag tatcatggtt
     4141 gtcaatatct tgattaccat tcgctttata caactctatc tgatgatacc ctttgcccct
     4201 gttaccattc cgacctttct tagtgatgac tggagaagtg ttgggattgg ttaccttaaa
     4261 aacatcatgg tctatgccgt tcaaggtatt ttgatttttc taattgtatc tcttgttccc
     4321 ttgtttgaat cggctggaaa gattgccgta tcaaatggag ctggagtgat ggaatcactc
     4381 gccattatgt ttggtggctt ggtacaggct atattgttaa tcattgcctt agttggcagt
     4441 caacgaaccg cccgaagtat tttggggatg taggaggaag ggagtcatta tctcgcttcc
     4501 tctttttata gcttacttag attggagaac atttatgaac actcgtgtct ttaaggatat
     4561 ctcaaaggtt caacacaggg catggctagg atttacaact agacaggtta tttttgtatt
     4621 tccagctgtc gccctaacca ttttggtact gggcttgaac ctattttatt ggcaatttgg
     4681 ggattggttt gtctatggtt ttgtttttag ctttaccatt ccactcatgt tatttggggt
     4741 ctatcgccct aatgatttac catttgaaac ctacctcaag taccgttttc attatgaact
     4801 aacagtacca gatcgtacat ttactggaag aaaaggagac caacgtgaaa aaattaaaac
     4861 acttaatgaa accaaagacc tcttctaatg acaaaaagca aaagacgaaa acgcagaagc
     4921 aggagatcag accttctacc gtaaatacgt tagcctatca agggcttttt cagaatggcc
     4981 ttatgcaggt cagtccaagc tatttctcac aaacctatct cttaggagat gtcaattatc
     5041 aaacagttgg cttagatgat aagggagcta ttgttgagaa atattccgat ttaatcaatt
     5101 cactggatga taagactaat ttccaactga ccatcctcaa tcaaaaggtc aatctggaaa
     5161 aattccgtaa gagtattctc tatcccttac aggaagatgg gtttgatgct tatcgtgatg
     5221 aattgaatcg catgatgaat gccaacttag aggcgggtga gaacaacttt tcagctgtca
     5281 agtttctttc atttggcaag agcgaccaaa caccaaaact agcttttcgt tctctatcac
     5341 agattgggga atatttcaaa agtggctttt cagagattga tgtatctctt ggtttacttg
     5401 gtggagagga gcgagtgaat gtccttgcgg atatgttacg aggtgaaaat catttaccgt
     5461 tttcttacaa ggatttgacc ctctcaggtc aatccaccaa acactttatt gccccaacct
     5521 acctttcctt taaacacaag aatcatattg aattggacga tcgattatta cagattgttt
     5581 atgttcgtga ctatggcatg gagttagggg ataaatttat tcgtgacctc atgcagtcag
     5641 atttagaagt gatgattagc ctacatgcta aaggctctac caagtctgaa accatgacca
     5701 agctgcgaac caagaagacc ttgatggaat cgcaaaagat tggggaacaa caaaagatgg
     5761 ctcggactgg aatctatttg gagaaggtcg ggcatgttct tgaaaacaat attgatgaag
     5821 ctgaggctct tcttcaaacc atgactcaaa caggagataa actgtttgac accgtatttc
     5881 taattggcgt gctggcggat actgaagacc aactcaaaca atctcttgat attatcaagc
     5941 aagtggctgg gtcaaatgat atgattatcg ataacttgac ctatatgcaa gaagctgcct
     6001 ttaatagtct cttgccattt gggaagaact atctcgaagg tatttctcgg tctctattga
     6061 cttcaaacat tgccgtgaat gccccttgga cttccgtaga tattcaggac aagggtggga
     6121 aattttatgg catcaatcaa atctcaagta atatcatcag tattgaccgt ggcaagttaa
     6181 atactccgtc aggtttgatt ttagggactt ctggagctgg caaagggatg gcgacaaaac
     6241 atgaaatcat ctcaactaag ctcaaggaag cagatagtga tactgaaatt attattgttg
     6301 acccagaaaa
//
LOCUS       DASFSW010000108         6270 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.108, whole genome shotgun sequence.
ACCESSION   DASFSW010000108 DASFSW010000000
VERSION     DASFSW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.108"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            72..1499
                     /locus_tag="U1099_001633"
     CDS             72..1499
                     /locus_tag="U1099_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEM4867944.1"
                     /translation="MKTVLAIDLGATSGRGILYSIENGKLISREVHRFNNSIQKVKGR
                     LCWDIESLVKSVKKCIRISQEQSELSSVGIDTWGVDFALLDVNGEMIAQPVSYRDRRT
                     ESILDEIARYSSLNDLYFKTGNQLMEINTLFQLIACKSQTPEEYFKADKLLMISDYLN
                     FVLTGEVAIERSIASTMQMINPLTQDWNHEILKTFEISELLLPKLVSEGNILGSISDE
                     ILNNEITVINVCQHDTASAVAAIPHEEENLLYVSCGTWSLIGTELKGPILTDSALKYN
                     FTNEAGYDGTTRFLKNCTGLWIIEELKKSFLKLGKDFSYDEITEMVNNVESCVPTFDT
                     DASTFASPGNMIEKIVCYFSDKGIKVSTDPAILFKIVYQSLAEKYKEVIIQLEEITGN
                     IYKRIDLIGGGSKSEYFSQLVADVTGKTVVTGLYEATSIGNALVQFKALGYVEDMKEA
                     KKLVKESITFNHFYPKKEEKNDTLS"
     gene            1483..3270
                     /locus_tag="U1099_001634"
     CDS             1483..3270
                     /locus_tag="U1099_001634"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEM4867945.1"
                     /translation="MTLYPKIGIRPTIDGRQGGVRESLEEKTMQMAMAAKDLIESNLR
                     YADGTPVQCLLASRTIGGQGDAGIVYDEFARDNVVATLSVTPSWCYGTETMDMDPHTI
                     KAIWGFNGTERPGAVYLAAAMSGYAQKGYPAFKIYGHDVQDLDDNSIPADVAEKILLF
                     AKGAIAAGQMKGKSYVNIGASSMGIAGSQVTTSFFEDYLGMLVEFVDMTEILRRMELE
                     IYDKEEYEKALAWIKEYCKEGVDINAGKEFPEVITKSKVIPADKDWEFIAKQTLIIRD
                     ILFGNPRLAEIGWEEEARGRNAISGGFQGQRQWTDWLPNGDFAEAILASTFDWNGSRP
                     VTAFATENDTLNATAMLFGTLLTNKAPIFSDVRTCWSPEAVKRVTGQELTGRAKDGIL
                     HLINSGASALDGSAAARDEAGQPTMKEFWNMTEEDIKACLEATDWCRANYEYFRGGGY
                     SSHFKTQGELPVTLIRVNLVKGVGPSLQIAEGYTCPLEDEVHRILDERTDKTWPTTWF
                     APNLGEPGFETVYDVMNHWGSNHGAFVHGHIGAEVITLASMLRIPVALHNIPRERIFR
                     PSIFDGVGTKDLEAADFKMCEVLGPLYSK"
     gene            3291..3929
                     /locus_tag="U1099_001635"
     CDS             3291..3929
                     /locus_tag="U1099_001635"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEM4867946.1"
                     /translation="MEKERKELIKYGKKLVTEGLTKGTGGNLSVFNREKGLMAITPSG
                     IDFFEIKEEDIVIMDLEGQVVEGEKLPSSEWYMHLIQYQQREDLDAVIHAHTTYGTVL
                     AVLREPLPASHYMIAVAGKDVRVAEYATYGTKELAENAAEAMKDRRAVFLANHGILAG
                     AQDLLNAFNIIEEVEYCSKIYCVAKSMGEPVILPDEEMELMAEKFKTYGQRK"
     gene            3946..5244
                     /locus_tag="U1099_001636"
     CDS             3946..5244
                     /locus_tag="U1099_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM4867947.1"
                     /translation="MKKGLLKCGALCLFASVILAACGSSSGTSSSSEKAASGDDTVLE
                     FYHGYHHSEEEWPAAKVMRDLYDEFAKKHATGDVEFKPTPVNGSLTDIMNNKVASGEF
                     PDMIDLAGNDVSLAAIEQSLVLDLKPYIDDNGLEKNVGLNYTQNDVNGKIYTVHDQLL
                     TLGLWYNADIFSKAGAKTPDQWTKWSDFTEAMALVRKQGVYAFGAGEPAIRLFNTVLG
                     SSEAGRKLLEGPLTSEGIDSKEFAEALKLVMTEVQANGSANAGGDANVYSTDFTENKS
                     AVFFNGVWAAGGLKDNAALKPGLYAGNVAISSAGGGITISSKLSEEEEKLALEFVKYM
                     TSDEVQTTIFEKVGANPASQAIDTAQIASGSDDATVKLLGEATSMANNADVTVPTVVS
                     VWGGDVRTAIINALTESAADGVNIDQKVKETQDILKALIG"
     gene            5299..6219
                     /locus_tag="U1099_001637"
     CDS             5299..6219
                     /locus_tag="U1099_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM4867948.1"
                     /translation="MAKKTMTLQNRKAQKQRFIFLFLLPTLVCFFLFYFYSVVTIFAT
                     SFTKWDYTNLTSPEFLGFGNLFANYKYIFSEYPFFLEALKNSITWAIIGVVVQVPLAL
                     SVAIALSKKLAGWKLSRNLYIIPSIISSAAMGLIFLQIYNPNYGVVNQVIRFFNPEFS
                     DAILLTPGLNIIAMTCAYIFFAGTSTIMILGQIFAIPQEIHEAAILDNITGWRREWYI
                     TIPMIKETLKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFIIRELAVSSSRTQYAR
                     ANTIGVIQILGGMLIILCINLIFKEGKRRK"
BASE COUNT         1981 a         1070 c         1421 g         1798 t
ORIGIN      
        1 tcaagtattg taatagaagt ttgtatatat taaaataaaa tcatgaaagc ggtaacagaa
       61 aggaggttca aatgaaaact gtattggcta ttgaccttgg agctacgtcg ggaagaggaa
      121 tcctatattc tattgaaaat ggaaaactga tttcgagaga agttcatcga ttcaacaatt
      181 caatacaaaa agtgaaggga agactgtgtt gggatataga aagcttggta aaaagtgtta
      241 aaaaatgtat cagaatttct caagaacagt cagaattatc ttctgttgga attgatacat
      301 ggggtgttga ttttgcatta ttggatgtaa atggagaaat gattgctcaa ccagtatcct
      361 atcgagatag gcgaactgaa tcaatattag atgagattgc aagatattct tctctaaatg
      421 atttgtattt taaaacgggt aatcagttga tggagattaa cacactattt caattgattg
      481 cttgtaaatc tcaaaccccc gaagaatatt tcaaagctga taaactgtta atgatttcag
      541 attacttaaa ttttgtattg acaggtgagg tcgcaattga aagaagcatt gcttccacaa
      601 tgcaaatgat taacccattg acacaagact ggaatcatga aattttaaaa acatttgaaa
      661 tttcagaatt acttttaccg aaactagtat ccgaaggaaa tatacttggt agtatatcgg
      721 acgaaatatt gaataatgaa ataacagtaa tcaatgtctg tcagcatgat acagcaagtg
      781 cggtagcagc gattccacat gaagaggaaa acttactcta tgtctcttgt ggaacttggt
      841 cacttatcgg gacagaactg aaaggcccaa tacttaccga tagtgcactg aagtataatt
      901 tcacaaatga agctggctat gatgggacta ctagattttt gaaaaattgt actggacttt
      961 ggattattga agaattaaaa aaatcgttct taaaattagg aaaagatttt tcatacgacg
     1021 aaattacaga gatggtgaac aatgtggaaa gttgtgttcc aacatttgac acagatgctt
     1081 cgacattcgc aagtccaggt aacatgattg aaaaaattgt ctgttatttt tcagataagg
     1141 ggataaaagt atcaacagac ccagcgattc tatttaagat agtttatcaa agtttggctg
     1201 aaaaatataa agaagtcatc atacaattgg aagagataac agggaacatc tataagagaa
     1261 ttgacttaat aggcggtggt tccaagtcag aatatttctc acaattagta gcagatgtta
     1321 caggtaagac ggtggttact ggtctgtatg aagcaacctc aatcggtaat gccctcgttc
     1381 aatttaaggc acttggctat gttgaagata tgaaagaagc caagaaactt gtaaaagagt
     1441 ctataacatt taaccatttt tatccaaaga aggaggaaaa aaatgacact ttatcctaaa
     1501 attggaatcc gtccgactat tgatggtcga cagggcggtg taagggaatc cttagaagaa
     1561 aaaaccatgc aaatggctat ggctgctaag gatttgattg agtccaattt gcgttacgca
     1621 gacggaaccc ctgttcaatg tttgctggcc agtcgaacca ttggtggtca aggggatgca
     1681 gggattgtct atgatgagtt tgctcgtgac aatgtagttg caaccttatc tgtcacacca
     1741 agttggtgct atggaacaga aaccatggat atggatccac atacgattaa agctatttgg
     1801 ggctttaatg ggacagagcg gcctggagca gtttatctag ccgcagccat gagtggctac
     1861 gctcaaaagg gttacccagc attcaagatt tatggtcatg atgttcagga tctggatgac
     1921 aatagcattc ctgctgatgt agctgaaaaa atcttgctat ttgccaaggg agccattgct
     1981 gctggtcaga tgaaaggcaa gtcctatgtc aacattggag cttcttcaat gggaattgcg
     2041 ggttctcagg ttaccacttc cttctttgaa gactatctgg gaatgttggt cgaatttgtg
     2101 gacatgactg agattcttcg ccgtatggag ttggaaatct acgataaaga agaatatgaa
     2161 aaagctcttg cttggattaa ggaatattgt aaagagggtg ttgacatcaa tgcaggaaag
     2221 gaattcccag aagtgattac caagtccaag gtgattccag ctgacaagga ttgggaattt
     2281 attgccaagc aaaccttgat tatccgcgat attctctttg gaaatcctcg cttggcagag
     2341 attggctggg aagaggaagc tcgtggcaga aatgcaattt caggtggttt ccaaggccaa
     2401 cggcaatgga ctgactggct accaaatggt gattttgctg aagcgattct ggcttccact
     2461 tttgactgga atggttcacg tcctgtgact gcctttgcaa cagaaaatga tactttgaat
     2521 gcgaccgcca tgttatttgg aaccctccta accaataagg ctccaatttt ctctgatgtt
     2581 cggacctgtt ggagcccaga ggcggtcaaa cgagtgactg gtcaagagct aacaggtagg
     2641 gctaaggatg gcatcctcca ccttattaac tcaggagctt cagcccttga tggaagtgca
     2701 gcagcacgag atgaagctgg tcagccaacc atgaaagagt tctggaatat gacagaggag
     2761 gatataaaag cctgtctgga agcgacagat tggtgtcgag caaactatga atatttccga
     2821 ggaggtggct attcttctca cttcaagact cagggggaac ttcctgtgac cttgattcgt
     2881 gtcaatctag tcaagggtgt tggtccaagt ttacaaattg cagaaggcta cacctgtcca
     2941 ttggaggatg aagtgcaccg gatcttggat gaacggacag ataaaacctg gccaacaacc
     3001 tggtttgctc cgaatcttgg agaacctggt tttgaaaccg tctatgatgt catgaaccat
     3061 tggggttcta accacggtgc ctttgttcat gggcacattg gtgcagaagt tatcactctg
     3121 gctagtatgt tgcgaattcc tgtagccctc cacaatattc caagggaacg aattttccgt
     3181 ccaagcattt ttgatggtgt cggtactaag gatttggaag cggcagattt caagatgtgt
     3241 gaggttttag gaccgctata tagtaaatag aaggagaaca ctttatgttc atggaaaaag
     3301 aaagaaagga acttataaag tacggcaaaa agttggttac tgaaggctta accaaaggta
     3361 ctggtgggaa tctgagcgta tttaaccgtg aaaaaggtct aatggcaatc actccatctg
     3421 gaattgattt ctttgaaatc aaggaagaag atatcgtcat catggacttg gaaggacaag
     3481 tagtcgaagg tgaaaagctt ccttctagtg agtggtatat gcacctgatt caataccagc
     3541 aacgagaaga ccttgatgca gtcattcatg cccataccac ttatggaact gttttggcag
     3601 tactaagaga gccgttacca gctagccatt atatgattgc ggtagctggt aaagatgtcc
     3661 gagtagcaga atatgccaca tatgggacta aggaattggc tgaaaatgct gcggaggcta
     3721 tgaaagacag acgtgcagtc tttcttgcca accacggtat cttggctggt gctcaagact
     3781 tactaaatgc cttcaatatc attgaggagg ttgaatactg ttcaaaaatt tattgtgttg
     3841 ctaaaagcat gggtgaacca gtcatcctcc cagatgaaga aatggaattg atggctgaaa
     3901 aatttaaaac ttacggacaa aggaaataaa acggaggaac ataaaatgaa aaaaggtttg
     3961 ttgaaatgcg gagcattgtg tttatttgct tcagtgattc tagctgcttg cggatcgtca
     4021 tcaggaacta gttcaagttc tgaaaaagca gcaagtggtg atgatacagt tttagagttc
     4081 tatcatggct atcaccatag cgaagaagaa tggccagctg ctaaagttat gcgtgatctt
     4141 tatgatgaat ttgcgaaaaa acatgcaacc ggtgatgttg aatttaaacc aactcctgtg
     4201 aacggaagct tgacagacat tatgaataac aaggttgcaa gtggcgagtt tccagatatg
     4261 attgacttgg ctggtaacga tgtttcgcta gcagctattg aacaaagttt ggttctggat
     4321 ttgaaaccat atatcgatga taatggattg gaaaaaaatg ttggtctaaa ctatacacaa
     4381 aatgatgtga acggaaaaat ctatacagtc cacgaccaat tattaacctt aggtctttgg
     4441 tacaatgcag atattttctc aaaagctggg gctaaaactc ctgaccaatg gaccaagtgg
     4501 agtgatttta ctgaagctat ggccttggtt cgtaaacaag gagtatatgc ctttggtgca
     4561 ggagagccgg caattcgtct ctttaacact gtacttggct catctgaagc tggccgtaaa
     4621 ctattagaag gtcctctaac ttctgaagga attgattcaa aagaatttgc agaagcactt
     4681 aaattggtta tgacagaagt gcaagcaaac ggttcagcta atgcaggcgg cgatgccaac
     4741 gtttattcaa ctgattttac tgaaaataaa tcagcagtct tcttcaacgg tgtatgggct
     4801 gcaggtggtt tgaaagataa tgcagctttg aaaccaggat tgtacgctgg taatgttgcg
     4861 attagttcag ctggtggcgg tattacaatt tcaagtaaac tatctgagga agaagaaaaa
     4921 cttgcattgg aatttgtaaa atacatgacc agtgacgagg ttcaaactac aattttcgaa
     4981 aaagtgggag cgaacccagc tagccaagcg attgatactg ctcaaattgc aagcggttct
     5041 gacgacgcta ctgtaaaact cttaggtgaa gcgacatcta tggcaaacaa tgcagatgta
     5101 actgttccta ctgttgtatc tgtttggggt ggtgacgttc gtactgcaat tatcaacgca
     5161 ttgaccgaaa gtgctgctga cggtgtcaat atcgatcaga aagtaaaaga aacacaagac
     5221 atcttgaaag cccttatcgg ctaaaataac agggctaagg atgtattcct tagccctttt
     5281 ttagaaagtg agtattttat ggcaaagaaa accatgacct tacaaaatag gaaggcacag
     5341 aaacaacgat ttatttttct atttttactc ccgacattag tttgtttctt tctcttctac
     5401 ttttattcgg tggtaactat ttttgcaaca tcctttacta agtgggatta tacgaattta
     5461 accagcccag aatttctagg ttttggcaat ctttttgcca actataagta catttttagt
     5521 gaatatcctt tctttttgga agcactgaaa aattcaatta catgggcaat tataggtgta
     5581 gtagttcaag tcccattagc tttatcagtt gctattgcct tatcaaaaaa attggctggt
     5641 tggaaactat ctcgaaacct ttatatcata cctagtatta tttctagtgc cgcaatgggt
     5701 ttgatttttc ttcaaatcta caatccaaat tatggagtag ttaaccaagt tatccgcttt
     5761 tttaaccctg aattttcgga tgcgatttta ttgacacctg gcttgaatat tattgcgatg
     5821 acttgtgctt atattttctt tgcagggaca tcgacaataa tgatattggg tcaaatcttc
     5881 gccattccac aagagatcca tgaagcagca atactagata acattacagg gtggagaaga
     5941 gaatggtata tcaccattcc aatgataaaa gaaacattaa agacagtttc gattatggcc
     6001 gcaacttcgg gtttcctatt gtataacgaa gtattcttcc taacaaatgg tgcagctgga
     6061 acaaaaagta tcagttttat cataagggag ttagcggtat ctagttctcg tacacaatat
     6121 gccagagcta acactatagg tgtgattcag attttaggcg gaatgctaat cattttgtgt
     6181 atcaatctga tattcaaaga aggtaaacgt agaaagtgag gtagagcata tggacaatcg
     6241 tttgaatggc ctttctaagt cagggaaatt
//
LOCUS       DASFSW010000109         6260 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.109, whole genome shotgun sequence.
ACCESSION   DASFSW010000109 DASFSW010000000
VERSION     DASFSW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.109"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(22..600)
                     /locus_tag="U1099_001638"
     CDS             complement(22..600)
                     /locus_tag="U1099_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM4867949.1"
                     /translation="MKKEIIYDCINLILSFLLCRWLFMTFLFYQFSNIFMTIDFWPTL
                     AAILLMTGFCFTLFRLVYKPHISRLTLWFFYACYGLLLVYLLFFKSMGVRGVNWNLLS
                     TFSQDLFLNPTILVFNLLLFLPLGLLFSFSWKKLSLFVGAILLVEACQFFFSLGFFDL
                     GDILLNTSGFALGNFLGQSAIAQSFKNRIQKK"
     gene            696..1823
                     /locus_tag="U1099_001639"
     CDS             696..1823
                     /locus_tag="U1099_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002961311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEM4867950.1"
                     /translation="MKKRPTSAYIHIPFCTQICYYCDFSKVFIKNQPVDEYLAALMEE
                     VKFYDLPALRTLYIGGGTPSALSADQLDYLLTNLEDLLDLSQLEEFTIEANPGDLTAD
                     KIAVLKKSKCNRVSLGVQTFDDRMLKKIGRSHNQAQIYETIATLKEAGFYNISIDLIY
                     ALPGQTMEQVVDNVAKALELDIPHMSLYSLILENHTVFMNRQRRGNLHLPNEDVESDM
                     FDYILQELEKNGFEHYEISNFTKPGFESRHNLMYWDNSEYYGLGAGASGYIDGMRYRN
                     RGPIQHYLKSIREKGHSRLHEEFLSQTEQMEEEMFLGLRKKTGVSIERFEEKFGISFE
                     DRYGQVVRDLKNEGLLQEEDRWLRMTKKGLFLGDTVAERFI"
     gene            2054..2794
                     /locus_tag="U1099_001640"
     CDS             2054..2794
                     /locus_tag="U1099_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEM4867951.1"
                     /translation="MGLTYQEEFTIPFDMVDVKQEIKLPDFISYCLGVSGRQSEELGR
                     SDLYVFQEFGLIWVVTDYELTIQRLPKYNETITIKTEAVAYNKFFCHRMFYIYDAAGN
                     LLLDILCYFVLIDFETRKVAPVPEDLIAPYQSEQVKKLPRAPKYQLLENPSVQEFPVR
                     YFDLDMNGHVNNGKYLEWMYEALGYDFLLCHVPKKIQLKYLKEVEATSLVSSRMVAEA
                     GGSQHEIVVDGHIHAQAVIEWRERHVAG"
     gene            2781..3398
                     /locus_tag="U1099_001641"
     CDS             2781..3398
                     /locus_tag="U1099_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM4867952.1"
                     /translation="MSQDKWLEWAVRLQALAQTGLAYGKDVYDMERFEEIRQIAAEML
                     VEPSGQPLEVVKDLFCNETGYQTPKLDTRAAIFQEDKILLVQENDGLWSLPGGWCDVD
                     QSVKDNVVKEVKEEAGLDVEAQRVVAILDKHKNNPAKSAHRVTKVFILCRLLGGEFQP
                     NSETVASGFFSLDDLPPLYLGKNTAEQLALCLEASRSEHWETRFD"
     gene            3408..4181
                     /locus_tag="U1099_001642"
     CDS             3408..4181
                     /locus_tag="U1099_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEM4867953.1"
                     /translation="MTYTGYLIDLDGTIYEGKKRIPAGERFIHRLQECQIPYLFVTNN
                     TTRRPEMVQAMLAENFNIETPLETIYTASLATVDYMNDLGKEKTVYVIGEDGLKSAIF
                     EAGYVEDTENPAYVVVGLDTQLTYEKLTIATLAIQKGATFIGTNPDLNIPTERGHLPG
                     AGSLIALLKAATRVEPTFIGKPEVIIMDKALEILGTERSQTVMVGDNYLTDIRAGIDN
                     GFPTLLVLTGFTKPEEVADLPLAPTHVLNSLDEWSFDEN"
     gene            4171..4800
                     /locus_tag="U1099_001643"
     CDS             4171..4800
                     /locus_tag="U1099_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEM4867954.1"
                     /translation="MRTKLQIIGTILFVLSAAVLGTIYLAWLVYPLEISFLGLEKVVY
                     MKVADISYNFNILMNYLTNPFASVLDMPNFSSSADGLKHFADVKHLFHLTQGIFILTL
                     PAFVLFVKNILLKGYGDLVKKVIFWTMLTPMIIGLLGVLVGFDQFFALFHTVLFPGDS
                     TWLFDPAKDPVIYILPQEFFLHCFVLFFVLYEFFFWTILAWTGKKNRIG"
     gene            4898..5074
                     /locus_tag="U1099_001644"
     CDS             4898..5074
                     /locus_tag="U1099_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEM4867955.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            5178..6191
                     /gene="galE"
                     /locus_tag="U1099_001645"
     CDS             5178..6191
                     /gene="galE"
                     /locus_tag="U1099_001645"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEM4867956.1"
                     /translation="MSILVTGGAGYIGSHTVVELLKLGKEVVIVDNLSNSSILVLDRI
                     ETITGKRPTFYELDVADKEALRQVFENENIEAAIHFAGYKAVGESVAKPIMYYENNIM
                     STLALVEVMTEFSVKKIVFSSSATVYGLNNPSPLVETMPTSATNPYGYTKVMLEQILR
                     DVEVADKEWSIALLRYFNPIGAHESGLIGEDPAGIPNNLMPFISQVAVGKREELSVFG
                     NDYDTIDGTGVRDYIHVIDLALGHIKALEKISTTTGVHTYNLGSGQGTSVLELVKAFE
                     KVNGVPVHYKLVDRRPGDVATCYANADKAWKELNWKTEKTIEDMCRDTWNWQSKNPNG
                     YEG"
BASE COUNT         1856 a         1197 c         1467 g         1740 t
ORIGIN      
        1 actaaaaatg accttgcggg gtcatttttt ctgtattcta tttttaaaag attgagcgat
       61 tgcggattgt cccaagaagt ttccaagggc aaagccagaa gtgttaagca agatgtcacc
      121 aaggtcaaag aagcccagtg aaaagaagaa ttgacaggct tcaactagca atatggcccc
      181 tacaaagaga gaaagttttt tccaagaaaa tgaaaaaagc aacccaagtg gtaaaaagag
      241 taagaggtta aaaaccaata tagtaggatt gagaaatagg tcttgagaaa aagtgctaag
      301 gagattccag ttgacacctc tgacgcccat gcttttgaaa aagagaagat agactagcag
      361 aagcccgtag caagcgtaga aaaaccaaag tgtcagtcgc gaaatgtggg gtttatagac
      421 aagtcgaaat agggtaaagc aaaatccggt cattaacagg attgcggcaa gtgtcggcca
      481 aaaatcaatc gtcatgaaaa tgtttgaaaa ttggtaaaaa agaaaggtca tgaagagcca
      541 gcggcaaagc agaaatgaca ggatcaggtt aatacagtcg tagataatct cttttttcat
      601 agaggatctc ctttttattt cattatatca ttatttgagg gggaggtcaa ggctgtagaa
      661 aatattttta tagacagtgt gctataatag ttggtatgaa aaaacgaccg acatctgcct
      721 atatccacat tccattttgt acccagattt gttactactg tgatttttcc aaggtcttta
      781 ttaagaatca gcctgttgac gagtatttgg cagctcttat ggaagaggtc aagttttacg
      841 acctgccagc tctccgcaca ctttatattg gaggcggaac accgtcagct ctctcggcag
      901 accagctaga ttatctcctg accaatctgg aggacctgct ggacttgtca cagctggaag
      961 aattcactat agaggccaat ccaggtgact tgacggcaga caagattgca gttctgaaaa
     1021 aatccaagtg caatcgggta tcgctgggcg ttcagacctt tgacgaccgc atgttgaaaa
     1081 aaatcggtcg cagccacaat caggcccaga tttatgaaac cattgctacc ctcaaggaag
     1141 caggatttta caatatttcc atcgacctga tttatgccct gcctggtcag accatggagc
     1201 aggtggtcga taatgtcgcc aaggccttgg agctggacat tccccacatg agcctctata
     1261 gcttgatttt ggagaaccat acggtcttca tgaaccgcca acgccgtggc aatcttcatc
     1321 tacccaacga agatgtggag tccgatatgt tcgactacat tcttcaggag ttggagaaaa
     1381 acggctttga gcactacgaa atctccaact tcaccaagcc cggctttgaa agccgccaca
     1441 atctcatgta ctgggacaac tcggagtatt atggtctagg agctggagca tctggctaca
     1501 tcgacggcat gcgctatcgc aaccgcggcc ccattcagca ctacctcaag tccatccgcg
     1561 aaaaaggcca ttctcgctta cacgaagaat tcctcagcca gaccgagcag atggaagaag
     1621 agatgttttt agggctacgg aaaaaaactg gcgtgtccat tgagcgattc gaggaaaagt
     1681 ttggcatttc ctttgaagac cgctacggtc aggtggtcag agacttgaaa aatgaaggtc
     1741 ttttgcaaga agaagaccgt tggcttcgga tgactaaaaa ggggttgttt ctgggcgata
     1801 cagttgcaga gcgtttcata tagtcattca gtttgttttt attacttcga cgagcgaaga
     1861 aacttcgttt ccttatttcc aacttcaaac actccactgg agtatttgaa ctcactttgc
     1921 cgtactctac gaatgttact tactatcgta agtacgattt ccaaccttca atagtccact
     1981 ggactattga agctacctgc agctgttgac gtgaactgaa agactatact ttaagactag
     2041 aaagatcaac attatgggac taacatacca agaagagttc accattcctt ttgatatggt
     2101 ggatgtcaaa caagaaatca aactacctga ctttatttcc tactgtcttg gtgtatcagg
     2161 tcggcagtcg gaagaactgg gtcgcagtga cctctatgtt tttcaggaat ttggcttgat
     2221 ttgggtggtg acagattatg aactgaccat tcaacgttta cctaagtaca atgaaaccat
     2281 taccattaag acagaagcgg ttgcctacaa caagtttttc tgtcatcgga tgttctatat
     2341 ctacgatgca gcaggcaatc ttttgttgga cattctctgc tattttgtcc tgattgattt
     2401 tgaaactcgc aaggtggcgc ctgtgccaga agatttgatt gctccttatc agtctgagca
     2461 ggtcaagaaa ttgcctcggg ctcccaagta tcagctcttg gaaaatccat ctgtccaaga
     2521 atttcctgtt cgctattttg acttggatat gaatgggcat gtcaataatg gcaagtattt
     2581 ggaatggatg tatgaagcac tgggctatga ttttctgctc tgccatgtcc ctaaaaaaat
     2641 acaactcaag tatcttaaag aggtagaggc aactagcttg gtgagttcgc gtatggtggc
     2701 agaagctggt ggtagtcagc atgagattgt ggtagatggg catatccatg cgcaagctgt
     2761 catagaatgg agggaacgtc atgtcgcagg ataagtggtt ggaatgggcc gtacgtttac
     2821 aagccttggc tcagacaggc ttggcctatg gaaaagatgt ttatgacatg gagcgatttg
     2881 aggaaatcag acagattgcg gcggaaatgt tggtagagcc atctggacag cccttggaag
     2941 tagtgaaaga cttgttttgc aatgaaacag gctatcagac acccaagctg gatacccgcg
     3001 cagctatttt ccaagaggat aaaatcctac tggttcagga aaacgatggg ctctggtcct
     3061 taccaggtgg ttggtgcgat gtggaccagt ctgtcaagga caatgtagtc aaggaagtaa
     3121 aggaagaagc ggggctggat gtggaggctc aacgtgtggt ggcgattttg gacaaacaca
     3181 agaacaatcc tgccaagtca gcccaccgcg tgaccaaggt cttcattcta tgtcgtctcc
     3241 ttggcgggga gtttcagccc aattcggaaa cagttgccag tggctttttc agtctagatg
     3301 acttaccacc gctctacctt gggaaaaata cagctgagca gttagcactt tgcttggaag
     3361 ctagtcggtc tgagcattgg gaaacacgat ttgattaagg aggaaaaatg acatatacag
     3421 gttatttgat tgatttggat ggtacgattt atgaagggaa aaagcggatt cctgctggtg
     3481 aacgttttat tcatcgcttg caagaatgtc agattcccta tttatttgtg accaacaata
     3541 ccacccgccg tcctgagatg gtgcaggcta tgttggctga aaactttaac attgagacac
     3601 cgcttgaaac aatctataca gccagtctgg cgacagtaga ttatatgaat gacttgggca
     3661 aggaaaagac agtctatgtc attggagaag acgggctcaa gtctgccatt tttgaggcag
     3721 gttatgtgga agacacggaa aatccagcct atgttgttgt cggtttggat actcagttga
     3781 cctatgaaaa actgaccatt gccaccttag ctattcaaaa gggagcgacc tttatcggta
     3841 ccaatcctga tttgaacatt ccgactgaaa gaggtcattt gccaggagca ggctcactta
     3901 ttgcgctttt gaaagctgct acccgagtag agccgacctt tatcggtaag cctgaagtca
     3961 ttatcatgga caaggctctg gagattttag gcacagaacg cagccagaca gttatggtag
     4021 gagataacta cctgacagat attcgtgcag ggattgacaa tggatttcca actcttttag
     4081 tcctgacagg atttaccaag ccagaagaag tagcagactt acccctggct ccaacccatg
     4141 tcctcaacag cctagatgaa tggagttttg atgagaacta agttacagat tatcggaacg
     4201 attttatttg tcttatcggc agcagttcta ggaaccatct acctagcttg gctagtttat
     4261 ccgcttgaga tttctttttt ggggttggaa aaagtggtct acatgaaggt ggcagatatt
     4321 tcctacaatt tcaatatcct gatgaactat ctaacaaatc cttttgcaag tgttttagac
     4381 atgccaaatt tttcatcctc agctgatggt ctgaaacatt ttgcggatgt gaaacattta
     4441 ttccatctaa ctcagggaat ttttatcctc accctccctg cctttgtgct ttttgtaaaa
     4501 aacattctgc taaaaggtta tggagacttg gttaaaaagg tcattttctg gaccatgctt
     4561 acgccaatga taatcggttt attaggagtt cttgttggct ttgatcagtt ttttgcattg
     4621 tttcatacgg tgctatttcc aggagattct acatggctat ttgaccctgc aaaggatcct
     4681 gtcatatata ttttgcccca agagtttttc ttgcattgct ttgtattatt ttttgtcctt
     4741 tacgaattct tcttttggac aatactggcg tggacaggaa aaaagaatag gataggataa
     4801 gaacgtaaaa ttcatatttt agaatgaaaa aaatattgac aaatgagttg aatcatagta
     4861 aaatgataaa gactttgaaa ttgaggggag tgaaaaaatg tcaaaaacag tagtacgtaa
     4921 gaacgaatca cttgatgatg ctcttcgtcg tttcaaacgt gcggttacta aagctggtac
     4981 tcttcaagaa acacgcaaac gcgaattcta cgaaaaacca tctgtaaaac gtaaacgtaa
     5041 atcagaagca gctcgcaagc gtaaaaaatt ctaattgaat ttgaaaacgg ccgaaaggct
     5101 gttttttgtt tactttttac aacaggtaac atataatagg gatatagaat tttgtctagg
     5161 tgaaaaggag taaaaagatg agtatcttag ttactggtgg tgcaggttat attggtagtc
     5221 atacggttgt cgaattgttg aagttgggga aagaagtggt tatcgtggat aatctttcaa
     5281 actcaagtat tttggtttta gatcgcattg aaaccatcac tggcaagcgc ccaactttct
     5341 atgagttgga cgtggcagat aaggaagcct tgcgccaagt ttttgaaaat gaaaacattg
     5401 aggcagccat tcactttgct ggctacaagg cagtaggaga atcagtggct aagccaatta
     5461 tgtactatga aaacaatatc atgtcaaccc tagctttggt ggaagtcatg acagaattta
     5521 gtgttaagaa aattgtcttc tcctcaagtg cgactgttta tggcctcaat aatccatcgc
     5581 ctttggttga aactatgcca actagcgcaa cgaatccgta cggctatacc aaagttatgt
     5641 tggaacaaat cttgcgtgat gtggaagtag cagataagga gtggagtatt gcattgctcc
     5701 gttatttcaa tccaatcggt gcccatgaat ctggcttgat tggggaagat ccagctggta
     5761 ttcctaataa tttgatgccg tttatttcac aggtagcagt tggtaaacgt gaggagctca
     5821 gcgtttttgg taacgattat gatacaattg atggtactgg agtgcgtgat tatatccatg
     5881 tgattgattt ggcactcggt catatcaagg cgcttgaaaa aatttcaact actacaggtg
     5941 tacatactta caatcttggt tcaggtcaag gaacaagtgt attagagttg gtcaaagcct
     6001 ttgaaaaggt aaatggcgtt ccagtacatt ataagcttgt tgaccgtcgt ccaggtgatg
     6061 tggcaacctg ttacgctaac gcagacaagg cttggaaaga gttgaactgg aaaacagaaa
     6121 aaacaattga agatatgtgc cgcgacacat ggaactggca atcgaaaaat ccaaatggat
     6181 acgaaggata agaaaagatg tgagtggagg ctcacatctt atttttgtaa ttatggaaga
     6241 gaggctgggc agaaagtcca
//
LOCUS       DASFSW010000110         6251 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.110, whole genome shotgun sequence.
ACCESSION   DASFSW010000110 DASFSW010000000
VERSION     DASFSW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.110"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            481..1446
                     /locus_tag="U1099_001646"
     CDS             481..1446
                     /locus_tag="U1099_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM4867957.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLIAPPKYTYKKEINRMVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKAYFQ
                     TALDFISERVGKQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKAILGNQKSLSEWQ
                     TAYHERMSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYEEIVSALSDINAFNA
                     GKKRDKALDLLSAWLPDVEKFSKEIGKQQAYIDSLKERIGQESDYAGRMRDEKYEQEL
                     KVQKANQKIFELQRTNEQMGRLLSKIPPEVLEELQKNHRSRAKER"
     gene            1455..2309
                     /locus_tag="U1099_001647"
     CDS             1455..2309
                     /locus_tag="U1099_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEM4867958.1"
                     /translation="MKKQDFKVLKTKDLYPFPDNPFHVAEDETLSELAESIKEFGIVT
                     PIITRPKEDGDGYEVIAGQRRVRASELAGINTVPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYKMKSEAMKRQGFRTDLTSSQVVTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKDEQENLFATMESEEATPSLSQAQRMKQLS
                     QSGRLDMDTIFAIMTEEKGNQKETLKINTSKLKKYFPKNTTPKQMEETIIKLLERELQ
                     RKRNRDSR"
     gene            2355..2585
                     /locus_tag="U1099_001648"
     CDS             2355..2585
                     /locus_tag="U1099_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867959.1"
                     /translation="MREIQYEIVKEIAVLSTGDSGYTKEINLISWNGKEPKYDIRSFS
                     PNREKCGKGITLNADEAAALLKALQKELNSED"
     gene            2585..2848
                     /locus_tag="U1099_001649"
     CDS             2585..2848
                     /locus_tag="U1099_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HEM4867960.1"
                     /translation="MVSDWQGGDISKLFPALSGKEDLSMGEDKKADKKRKRIVPKAPV
                     QMIISREYVGTQTVTEAFVPIISEDIRKKIAEGDTFDNEGLSA"
     gene            2903..4759
                     /locus_tag="U1099_001650"
     CDS             2903..4759
                     /locus_tag="U1099_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM4867961.1"
                     /translation="MAGIKEEKKIYLVGIYCRLSKDDGTDNESASIATQKSILTDYVK
                     KQGWHIAKTYVDDGYSGTNFQRPSFQNMIKDIESGLINCVITKDLSRLGRNYLDCGLY
                     LEVFFPEHNVRYIAVNDGVDTLNKSAMDITPFRNILNEMYAADISVKIKSAYRARFQQ
                     GKFMGTTATYGYIKDPADHNHLLIDDKVAHVVKEIFDLALKGNGVAKICRHLNKQHIL
                     RPAAYAAERGETGFERHFEGNEDKRYIWSGNSVRSILRSPIYAGNLVGYKRIAANMKS
                     KKRPSKLPEEWEVIPNTHEGIVTQEEFDIVQQLITSRRLPQNKGGFVNIFAGVIKCVD
                     CGCALRAMNVHRRKRPEIIDCVQYSCNNYARNGRSECSAHNIEARDLFNAVLADINCF
                     ADMAVNDEKAVRAIEKRLTETDQSRAKALEKERKKLNKRLAELDRLFSSLYEDKVMER
                     ITERNFEMMSGKYQKEQLEIEARLKEVTETLNESYEKSRGIRDFLALIRNYQGLKELD
                     ATVINALIDKILVSEREKMADGTVKQEIKIYYKFIGFVDELHIIPTKRWAAMPAKNCT
                     VCGVEYVPGSGASRYCPACAKKIRREKSNESKRRSREQKRIACMNCPQKMTD"
     gene            4752..5711
                     /locus_tag="U1099_001651"
                     /pseudo
     CDS             4752..5711
                     /locus_tag="U1099_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5782..6081
                     /locus_tag="U1099_001652"
     CDS             5782..6081
                     /locus_tag="U1099_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867962.1"
                     /translation="MAQTLEEMRYQLEEWLAQGFTSPEDRANYQALKEQYEDEMLDYS
                     FSKREITGQLELIITSRENDFPSLDEVTKAEYLDLIAQLDELDQEQANYYRKQLA"
BASE COUNT         2009 a         1292 c         1567 g         1383 t
ORIGIN      
        1 cgatattagg atacccccct gttactgtca ttgacgctta aagccatgta aagctggcgg
       61 ctctgccctg cgggtgacag cagtgacggt ggtgacagtg attttaggat accctgccgc
      121 tgtcatccct acgggagaac accccgtaaa cgcaaaatgc aggcgtgaga tttactcgcc
      181 cttttcggtc gtgtaaaacc acccctgcgg tcagaaaaat cattccgatt tttccgactg
      241 cgtttacagg gtgtaacaca ctacactttg ccctgcaaag tcgtgtgcca gacgttccct
      301 ctggactccc ttaaggcagg gctgtgccct gctatcctac gccttacggc ttcggataaa
      361 agaatggcgg catgacggtg acggctcttg cgaggggggt atcccaaaac tgctgtcacc
      421 accgtcaccg ctgtcacccc aaaggacggt cacccgccga aagaaaggag gaatccgcct
      481 atgccttatg caatcctgcg tttccagaaa cgaaaagcgg gcggcgttgc ggcttgtgaa
      541 cgccacaacg agcggaagaa agaagcctac aaaagcaacc cagatataga tatggaacgc
      601 tctaaaaaca attaccatct catagcacca ccaaagtaca cctacaagaa agagattaac
      661 cgcatggtag ccgaagcggg gtgcaggaca aggaaagaca gcgtgatgat ggtggaaacg
      721 ctcatcacag cttcaccaga atttatgaac cagttaccgc ccgaagaaca aaaagcgtat
      781 ttccagacgg ctcttgactt catttcggag cgtgttggaa agcagaatat cctctccgct
      841 gtcgtccata tggacgagag aacgccccat atgcacctct gctttgtgcc gattacgcca
      901 gacaataagc tgtcagcgaa agctatctta ggcaaccaga aatcattatc cgagtggcag
      961 accgcctacc atgagcggat gtcctcacgg tggaatcagc ttgaacgggg gcagtcctca
     1021 atggaaacca agcggaaaca cgtccccaca tggctctata aattaggcgg caggcttgat
     1081 aaacagtatg aagaaatcgt gtctgcccta tccgacatca acgcctttaa cgcagggaag
     1141 aaaagggata aagcgttaga tttactctct gcatggctgc cagacgtgga gaaattctct
     1201 aaggaaatcg ggaaacagca ggcgtatatc gacagtttga aagagagaat tgggcaggaa
     1261 tcagactatg cggggcgtat gcgtgatgaa aagtacgagc aggaactaaa ggtgcagaaa
     1321 gcgaatcaga agatatttga attgcagaga accaacgagc agatggggcg gctgctgtca
     1381 aaaataccgc ccgaagtgtt ggaagaattg cagaaaaatc atagaagcag agcgaaagaa
     1441 aggtagatat gtgaatgaag aaacaggatt ttaaggtgtt aaagaccaaa gacttgtacc
     1501 cgttccccga caatccgttt catgtggcag aagatgaaac actgtcagag ttagcggaaa
     1561 gcatcaagga atttggcatt gtcacgccga taatcacacg cccgaaagag gacggggacg
     1621 gttatgaagt gattgcagga cagcggcgtg tccgtgcttc tgaacttgca gggataaata
     1681 ccgtgcctgc gtttgtcctg cccttagacc gtgaccgagc catcatcacc cttgtagaca
     1741 gcaatttgca gcgtgagaat atcctgccat cggagcgggc gtttgcttac aagatgaaat
     1801 ccgaagccat gaagcggcag ggtttccgca cagacttaac ctcgtcacaa gttgtgacga
     1861 agttgcggac ggacgacaag gtggcacagg gcttcggcgt gggcaggatg accgtgcagc
     1921 gttttatccg cctgacggaa ctgataccgc cgattttgca gatggtggac gaggggaaaa
     1981 tcgccctcac gcctgcggtg gaactgtcct tcttgaagaa agacgagcag gaaaacctct
     2041 ttgccacgat ggagagcgaa gaagcaacgc cctcactctc acaggcacag cggatgaaac
     2101 agttaagcca gagcgggcgg cttgacatgg atacgatatt tgcgattatg acggaggaaa
     2161 agggcaacca gaaagaaacc ttgaaaatca acacaagcaa gctgaaaaaa tactttccga
     2221 agaacacaac gccgaagcag atggaggaaa ccatcatcaa acttttggag cgtgagttgc
     2281 agaggaaacg caaccgtgac agccgctaat cttctttttt cgggaagtaa atacagagag
     2341 ttgaggtatg gagaatgaga gaaatccagt atgaaatcgt aaaggaaatc gcagtattgt
     2401 ctacgggcga cagtggctac acaaaggaaa tcaatctcat ttcatggaat gggaaagagc
     2461 cgaagtatga catccgcagc ttttccccga accgtgaaaa gtgcggcaag ggaatcacgc
     2521 tgaacgctga tgaagcggcg gcactcctta aagcattaca gaaagaatta aacagcgagg
     2581 attaatggta tctgattggc agggcgggga catttccaaa ctcttccctg ccctgtctgg
     2641 aaaggaagat ttaagtatgg gcgaggataa gaaagcagat aaaaagagaa agcgtatcgt
     2701 gccaaaagca ccagtgcaga tgataatcag ccgtgaatat gtcggcacac agaccgttac
     2761 agaagcgttt gtcccgatta tctccgagga tattcggaag aagattgccg agggcgacac
     2821 cttcgacaat gaggggctgt ccgcttagaa tgtacgcaat gggacatgaa aacagatagg
     2881 acagatacgg aggttttaca gtatggcagg aatcaaagaa gaaaagaaaa tctatttagt
     2941 cggcatttat tgccgcttat ctaaagacga tggtacggat aacgagagtg cgagcattgc
     3001 gacacagaaa tccatcctca cggattatgt gaaaaagcag ggatggcaca tagcaaaaac
     3061 gtatgtggac gatggttatt ctggtacaaa tttccaaaga ccaagtttcc agaatatgat
     3121 aaaagacatt gaaagcggtc tgataaactg cgtgattacg aaagatttat cccgtctggg
     3181 gagaaactat cttgattgtg ggttatatct ggaagttttc ttcccagagc ataacgtgag
     3241 gtatatagcg gtcaatgacg gcgtagatac cttgaataaa tctgctatgg acatcacgcc
     3301 tttccgcaac attttaaacg aaatgtatgc cgctgacata tctgttaaga taaaatcggc
     3361 atatcgggcg aggtttcaac aggggaaatt catgggaact accgccactt atggctatat
     3421 caaagaccct gccgaccaca accatctgct gatagatgat aaagtggcac atgttgtaaa
     3481 agagatattc gaccttgcat taaaagggaa tggagttgcc aaaatttgca gacatcttaa
     3541 taaacagcat atcctacgcc ctgccgctta tgcggcggag cgtggcgaaa caggctttga
     3601 acgtcatttt gaggggaacg aggacaaacg ctatatttgg agtgggaaca gcgtgaggag
     3661 cattttaaga agcccgatat atgcgggaaa tcttgtaggc tacaaacgga ttgccgctaa
     3721 tatgaaaagc aagaaacgcc cctctaagct gcccgaagaa tgggaagtga tacccaatac
     3781 ccatgaggga atagtcacgc aggaggaatt tgatattgtc caacagctta ttacaagtcg
     3841 taggcttcca cagaacaagg gaggatttgt aaatattttt gcaggcgtta tcaagtgtgt
     3901 ggactgcgga tgtgctctgc gggcaatgaa cgtacacagg aggaaacgcc cagagattat
     3961 cgactgtgta cagtattcat gtaataatta tgcaagaaac ggaagaagcg agtgtagtgc
     4021 ccacaatata gaagcaaggg atttattcaa tgccgttctt gccgacatca actgttttgc
     4081 ggatatggca gtgaatgatg aaaaggcggt cagggccata gaaaagcggc tcacggaaac
     4141 agaccagagc agggcgaaag cattagagaa agaacgtaag aagctgaaca aacgccttgc
     4201 ggaactggac aggctgtttt cctctctcta cgaggataag gtcatggagc gtattaccga
     4261 gcggaatttt gagatgatgt cggggaaata ccagaaagag cagcttgaaa ttgaagcaag
     4321 gctgaaagag gtgacggaaa ctcttaatga aagctacgag aaatcacggg gaatccgtga
     4381 cttcctcgcc cttatccgaa attatcaagg cttaaaagaa ctggatgcaa cagttataaa
     4441 cgcactcata gacaagatac ttgtttcgga gcgtgagaag atggcagacg gaacagtgaa
     4501 gcaggaaatc aagatttact ataaattcat cggctttgtc gatgaattac atatcatacc
     4561 tacaaaacgg tgggcagcaa tgcccgctaa aaattgtacg gtgtgcggcg ttgaatatgt
     4621 cccgggctct ggtgcatcaa ggtattgtcc tgcttgtgcc aagaagatac ggagggagaa
     4681 atcaaacgag agcaaacgca ggagcagaga acagaaaagg atagcatgta tgaactgtcc
     4741 gcaaaaaatg accgactgac attgtccagc gaaatggtag attgattcgt cagggaaaca
     4801 tgcaccagga ggtagatatt tatcactata ttaccaaagg gagcttcgac aattatcttt
     4861 ggcagacaca ggagaataag ctcaagtata tcacccagat tatgacctca aaagatcctg
     4921 tgagatcagc tgaagacatt gatgaacaaa ccatgacggc ttctgacttt aaggccttgg
     4981 caactggaaa cccttatctc aaactcaaaa tggagttgga aaatgaactg acagttttag
     5041 agaatcaaaa acgagccttt aatcgctcca aagacgagta tcgccatacc atttcctata
     5101 gcgagaagca cctccctatt atggaaaaac ggttgagtca atatgataaa gatattgccc
     5161 aatctttggc aacaaagtcg caagattttg tcatgcgatt tgacaatcaa gcaatgaata
     5221 atcgtgcaga agcaggagac tacctgcgta aactcatcac ctataaccgc tccgaaacta
     5281 aggaagttcg gactctggcc aactttagag gatttgattt gaaaatgaca acacgtggtc
     5341 ctagtgagcc cttgccagag actgtctctt taatgattgt aggagataat cagtatactg
     5401 tcgcccttga tttgaaatca gacgtgggaa ccattcaacg gattagtaat gccattgacc
     5461 atattattga tgaccaagaa aagacgcaag agctggtaaa ggatttaaaa gataaactac
     5521 gagttgccaa agtagaagtt gagaaagtct tccccaagga agaagattat cagcttgtaa
     5581 agtctaagta tgatgttttg gctcccttgg ttgaaaaaga agcagaaatt gaggaaatag
     5641 atgcagcttt ggccaagttt agtgaagata caacacccca aatgaagcaa caaatagcac
     5701 tcgagatata agaaaaaagg ctgacaaata tttttgaacg cggtaaaatg aaagcaagaa
     5761 atcacaaaag gagaatagat catggcccaa acactagaag aaatgcgtta tcaactagaa
     5821 gaatggttgg cacaaggctt tacaagccca gaggatagag ccaactacca agctttaaag
     5881 gaacagtatg aagatgaaat gcttgattat agcttctcaa agcgtgaaat tactggacag
     5941 ctggaactca tcatcacaag ccgtgagaat gattttccaa gcttagatga ggtgacgaag
     6001 gcagaatacc ttgatttgat tgctcaacta gatgaacttg accaggaaca agccaactat
     6061 taccgtaagc aattagccta gaaagaggtg taagatgttg gaacaaattc tacaaagctt
     6121 tctgattatc atagcaatag gactgatgtt gcttgtcctt tatcagattg tgaaagtttc
     6181 aggtgcttta tttcttatcg gactcatcag tggaataatc tttattgaaa tctatggaat
     6241 ttacctcttt t
//
LOCUS       DASFSW010000111         6203 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.111, whole genome shotgun sequence.
ACCESSION   DASFSW010000111 DASFSW010000000
VERSION     DASFSW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.111"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(565..1764)
                     /locus_tag="U1099_001653"
     CDS             complement(565..1764)
                     /locus_tag="U1099_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEM4867963.1"
                     /translation="MAKLTVKDVELKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYILEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQEVAFIAGATRGAELE
                     AAINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAH
                     ASNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLE
                     KADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANA
                     FAGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQA
                     GTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPG
                     LAALTEK"
     gene            complement(2024..3034)
                     /gene="gap"
                     /locus_tag="U1099_001654"
     CDS             complement(2024..3034)
                     /gene="gap"
                     /locus_tag="U1099_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEM4867964.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKDGGFEVNGKFVKVSAEREPGNIDWATDGVDIVLEATGFFAS
                     KEKAEQHIHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGVQKGLMTTIHGYTGDQMVLDGPHRGGDLRRARAAAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLDKKVTAEEVNAAMKAAATESFGYTED
                     QIVSSDIVGISFGSLFDATQTKVIEVDGEQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(3401..5392)
                     /locus_tag="U1099_001655"
     CDS             complement(3401..5392)
                     /locus_tag="U1099_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEM4867965.1"
                     /translation="MRKKLKFSLVAVACATFLTACGTSNSSAPKAGEATPQTDFHSYI
                     NEQWLKDTKLGAGESQIDNLSQLSEKTQEAIQEMIDQLDMDYSKLQAGSDEKKLIDFY
                     RLAADFDTRNKLGLEPIKPYLEEIKKASTMEELNKILVALYGKNIRNLLHINVTQDIK
                     DSNKNTIYVDPHKLSFPKENYDGTDEFSLKNQKAFKAYLQEVFSLAGESSKEASSKAD
                     LLFNLEKEMAAVQRPKEEANNFEAMYNEKTWDEVKALVPNLPIAELVSELGLQNAPTT
                     VVSEPDALKKLNELYQEKNLDALKALLQYRVIAHYNDYLSSNLIEAKAKYDGVATGAV
                     DTPSQEEVAQAAIDANFSDMIGKVYVKHHFSEESKANILSMVQEIKATYAERIKAVQW
                     MSEETKMRALKKLDTLVVKIGYPDQWKDYSQLAIQSKEEGGSLLSNFDMVKTLELREE
                     MGQFGKPFDKSQFGMPSHTVNAYYSPTNNEIVFPAGILQAPLYDPKASPEENLGGIGA
                     VIGHEISHAFDKAGSQFDEKGNLVNWWKPEDLEKFNKKVQQAADIYSKLEVAPGYYVN
                     GEISTGEIMADLGGLTVAIDIAQKKGYDTKKVFESYGKAWREVTTKEFAIANISDEHP
                     PAKYRVNNIVNQIDQFYTDFNVKEGDPMYVKPEDRLRVW"
BASE COUNT         1755 a         1368 c         1129 g         1951 t
ORIGIN      
        1 aaagaactcc caactttaca aaaaaataag tatcctatag ttgattataa tacgagacaa
       61 gaagccacaa gcataagtcg agcttaaaaa ggtgagttta cgaagtaata ctcaatgaaa
      121 atcaaaaaaa tgattacaca acaaaatttt tcctccgagc cgacacttca gagcgtaaat
      181 tgcatttaaa aagagaccgg agctcaagcc ccagcctcat ttcgtcgcaa ttttttactt
      241 ttcagtaaga gctgcaagtc ctggaagcac ttttcacctt gctagacttt ctaactcgct
      301 tagcttcgta gaaactctac gcgatgcaca aaatgcaacg gaaatccgtt gcattttagc
      361 ctttacctag gctgattttc taaactcact acgttcacag aaaatctagc ctttgtacta
      421 gaaaagagtt ggtctcccaa ctcttttcat cgttcatctt tcatacgcag tagctgaatg
      481 gcagttcact ttgctacgct tgtgaccaac ctattcagcc ttgcgggggt gcgtctacgg
      541 aatcatagat tcctgactta ccgcctattt ttcagtcaaa gctgcaagtc ctggaagcac
      601 tttaccttcg aggagttcca ttgatgcacc accaccagtt gagatccatg agaacttgtc
      661 tgcacggcca aggttgatcg cagctgccgc tgagtcacca ccaccgatga ttgatttcac
      721 gcctggttgt ttcacgatag cgtccattac accgattgta ccagcttgga agtctgggtt
      781 ttcaaataca cccataggtc cgttccaaac aactgttttt gcaccagtca acgcttcgtc
      841 aaacttagcg attgacttag gaccgatgtc aagaccaagg aagccttctg aaactgcttc
      901 accttcagtg tcgcgaactt cagtgtatcc agcaaatgca tttgcttctt ttgagtcaac
      961 tggcaagatc aatttaccat tcgctttctc aagaagcgcc tttgccacat ccaatttatc
     1021 ttcttctaca agtgagttac caatttcgat accttgagct ttgtagaatg tgtaagtcat
     1081 accaccaccg ataagaactt tgtctgcttt ttcaagaagg ttctcgataa caccaatctt
     1141 atcagatact tttgaaccac caaggattgc cacaaatgga cgctctggag tttcaacagc
     1201 ttcttggatg tatgcaattt cgttttccaa aaggaaacca gctactgctt tttctacgtt
     1261 tgctgagata ccaacgtttg atgcgtgtgc acggtgtgca gtaccaaatg catcgttaac
     1321 gaagatacca tcaccaagtg aagcccagta tttaccaagt tcttcgtcgt ttttagattc
     1381 tttcttacca tcaacgtctt cgaaacgagt gttttcaaca aggagaactt gtccatcttc
     1441 caaagcattg atagccgctt ccaattcagc accacgagta gcaccagcga tgaaagcaac
     1501 ttcttgaccc aatttagctg ccaagtcagc tgctacagga gccaatgatt taccttcttt
     1561 gtcagcttct tctttcacac gaccaaggtg agagaaaaga attgcacgtc caccttgctc
     1621 aagaatatac ttgatagttg gaagagctgc tgtaatacgg ttatcgttag tgataacgcc
     1681 atctttcaaa ggcacgttga agtccacacg aacaagaact tttttgcctt tcaattctac
     1741 atctttaaca gtcaattttg ccattagata agaactcctt aaattatatt tactttacca
     1801 ttgtatcata ttttctgtat tttttcttgc ataaagatat ttttttcaca aatgttatga
     1861 aatctaaaac tatcacctcg taatcagatt tttcaaattc taaaatcgcc aaatcagaaa
     1921 caactcatct cattcatatt tttagcataa gaaaacagcc atattccagc aatccgaaaa
     1981 tacaaaaaaa agaggccgaa gcctcttcga ttttcactaa ggattattta gcgatttttg
     2041 cgaagtactc aagagtacga acaagttgtg cagtgtaaga catttcgttg tcgtaccatg
     2101 aaacaacttt aaccaattgc tcgccatcaa cttcgataac tttagtttga gttgcatcaa
     2161 acaatgaacc gaatgagata cctacgatat ctgaagatac gatttggtct tcagtgtaac
     2221 caaatgattc agtagcagca gctttcatag cagcatttac ttcttcagca gttactttct
     2281 tgtcaagagt tgcaaccaat tcagttacag aacctgttgg aactggaaca cgttgtgcag
     2341 caccgtcaag tttaccgttc aattctggga ttaccaagcc gatagcttta gcagcaccag
     2401 ttgagttagg aacgatgttt gcagcagcag cacgtgcacg acgaaggtca cctccacggt
     2461 gtggtccgtc aagaaccatt tggtcaccag tgtaaccgtg gattgtagtc atcaaacctt
     2521 tttgaacgcc aaatgcatcg tgaagagctt tagccattgg tgccaaacag tttgtagtac
     2581 atgaagcacc tgagataact gtttcagtac catcaaggat gtcgtggtta gtgttgaaaa
     2641 caacagtctt aacatcgtta ccaccaggag cagtgataac aactttttta gcaccgttag
     2701 cgtggatgtg ttgctcagct ttttctttag aagcaaagaa acctgtagct tccaaaacga
     2761 tatctacgcc atcagtagcc cagtcgatgt ttcctggctc acgctcagca gaaactttaa
     2821 cgaatttacc gttaacttcg aaaccaccgt ctttaacttc aacagtaccg tcgaaacgac
     2881 cttgagttgt gtcatatttc aacaagtgtg caagcattac tggatctgta aggtcgttga
     2941 tacgagtaac ttcaacacct tctacgtttt ggatacgacg gaaagcaaga cgaccgatac
     3001 gtccgaaacc gttaatacca actttaacta ccatgaatga tttcctcctt atgaaaatcg
     3061 agttctttat tttaaaaggc aagcctttta atccttttgt gaaaatatta acttgtaagc
     3121 ctacaagcat cttttcaacg tttttattat ataactattt accgaaaatt gcaactgctt
     3181 accggttcat cttatgtaaa aaatagaagg ccaaaaaata aaagttaaaa atcatagcca
     3241 tgcgttttct cgggtggtta tgactggtta aatacttaac ttaatagcat tgccatacct
     3301 cgacacattt tttaccgatt cttggtttac cgctacttct attttttacc taaaaaggtg
     3361 tctaccgccc attttggacc gcagacacct ctataatctt ttaccacact cgtaggcggt
     3421 cttctggttt cacatacatt ggatcccctt ctttgacatt gaagtctgta tagaattgat
     3481 caatttggtt gacaatgttg ttaacccgat atttagctgg tggatgttcg tcgctgatgt
     3541 tggcaatagc aaattctttg gtggtgactt cacgccatgc tttaccataa gactcgaaaa
     3601 ctttcttcgt atcgtagcct tttttctgag caatatcaat ggcaaccgtt aaaccaccga
     3661 gatccgccat gatttcaccg gttgaaattt cgccattgac atagtagccc ggagccactt
     3721 ccaatttaga atagatatct gctgcctgtt gaaccttttt attaaatttt tcaaggtctt
     3781 ctggtttcca ccagttgaca aggttcccct tttcatcaaa ttgagaacca gccttatcaa
     3841 aggcatggga aatttcatga ccgataacag ccccgattcc acctagattt tcttcgggac
     3901 tagcttttgg atcgtagaga ggagcctgta aaatccccgc tggaaagaca atttcgttgt
     3961 tagttgggct gtaataggca ttcaccgtgt gagaaggcat gccgaactgc gacttatcaa
     4021 aaggcttgcc aaattgcccc atctcttcac gcaattcaag tgtttttacc atatcgaagt
     4081 tagaaagaag acttccccct tcttccttgg attggatagc aagttgtgag taatccttcc
     4141 actggtctgg atagccgatt ttaacaacca atgtatctag ttttttcaag gcccgcatct
     4201 tggtttcttc agacatccac tgcactgctt tgattcgctc tgcataagtc gctttaatct
     4261 cttgaaccat agataggata ttagccttgg attcttctga aaaatggtgt ttaacgtaaa
     4321 ctttgccaat catatcggag aaatttgcat caatggccgc ttgagcaact tcttcctgac
     4381 tcggagtatc taccgcacca gttgctacgc cgtcgtattt cgcttttgcc tcgattaaat
     4441 tgcttgacag ataatcattg taatgggcaa tcacacgata ttggagcagg gctttaagag
     4501 cgtctaaatt cttttcttga tagagttcat ttaatttttt tagagcatct ggttctgata
     4561 cgactgtcgt tggagcattc tgaagtccta actcagaaac caactcagca atcggtaggt
     4621 tgggaaccag tgctttgacc tcatcccaag tcttctcgtt atacatggct tcaaagttat
     4681 tggcttcttc ttttggccgt tgcactgcag ccatctcttt ttccaaatta aagaggaggt
     4741 ctgccttaga agaggcttct tttgaggatt ctcctgccaa gctaaacact tcctgcagat
     4801 aggctttaaa ggctttttgg tttttcagtg aaaattcatc tgtgccatca taattttctt
     4861 ttggaaaact taacttgtga gggtcaacgt agatggtgtt tttgttactg tcttttatat
     4921 cttgagtaac gtttatatga agcaaattgc ggatgttttt gccgtatagg gctaccagaa
     4981 tcttgttgag ttcttccatg gtagaggctt ttttaatctc ctctaaataa ggcttgatag
     5041 gctctaaccc caatttgttg cgagtatcaa aatcggctgc caatcggtaa aaatcaataa
     5101 gttttttctc atcactacca gcttgcagct tggaatagtc catatccagc tggtcaatca
     5161 tctcctggat tgcttcttgc gttttctcag agagttgact cagattatct atttgcgatt
     5221 caccggcacc taattttgta tctttcaacc attgctcatt gatataagaa tgaaaatccg
     5281 tctgtggtgt tgcttcacct gctttgggag ccgaggaatt acttgtacca caggctgtca
     5341 aaaatgtcgc acaggcgact gctactaatg aaaattttaa tttttttctc atgatttctg
     5401 tcctttcttt ttattgttaa tgtgcttagt accactattt catcaccata caagcatcct
     5461 cctttagtaa ttagcggtcc taaactactc tttcatcgat ctaccttata aggattctaa
     5521 catttatatc tgcttttctt ctgtagccta tctattaatt ctcttatttt ttgtaaccat
     5581 tcccaaacaa agtaattcta aatgatttaa aacatttatt ttagcaaatt gagtgagagt
     5641 aggcaagaac tcgaacggtc aaaagagttc agcaacaatt cctaatttca agttattaag
     5701 ctaaaacaat ctattcacag accgttttaa tccgtttatt tcacagtagc cagacctaga
     5761 agtcactgtt gagttcatcg gcaacaggaa tcttataccc aatgaaatcc caaaatagca
     5821 aaccctgatc ttttgttagc catccacacc taaattaggg tattttttta taaattgtag
     5881 ctttctcatt gtatatttcc aaaaaggatc tgacggatac ctatttttta gtcgtttagt
     5941 ttgtttttag cactcacttc aaaggttcag ggaacctttg aaggctagag atagggcgaa
     6001 gcaagttcgc ctcaatagct gcaggttgct agaacagcct ttaggctgtt ctaagttaga
     6061 aataagactt gcctcgcgaa tcacctctgt aaagtacggc aaagcgagtt caaatactcc
     6121 agtgaagtgt tcgaagttga aaatagggaa acgaagtttc atcgctcgtc gaagtaatca
     6181 aagataaact actgatactc aat
//
LOCUS       DASFSW010000112         6184 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.112, whole genome shotgun sequence.
ACCESSION   DASFSW010000112 DASFSW010000000
VERSION     DASFSW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.112"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            363..1553
                     /locus_tag="U1099_001656"
     CDS             363..1553
                     /locus_tag="U1099_001656"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEM4867966.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTKGEYGFSAESVGVHPSLVEQSP
                     DIARGVNEALETRQDASQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLSHKLVRRL
                     AELRKSGEIAYLRPDAKSQVTVEYDEDNQPVRVDTVVISTQHDPEVSQEQIRQDVIER
                     VIKEIIPAHYLDDQTNYFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAHPVSVRIDTFGTSTVA
                     ESKLEAAVRQIFDLRPAGIIQMLDLNRPIYRQTAAYGHMGRTDIDLPWEKLDKVEALK
                     TAVL"
     gene            1584..2198
                     /locus_tag="U1099_001657"
     CDS             1584..2198
                     /locus_tag="U1099_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM4867967.1"
                     /translation="MKALIVFAHPRKESFTHALVDRVAEALLENGAEVVIRDLYEIGF
                     DPVLRGEDTIHIEDGKFIRQATVFPADVQVEMDLIAESDLLVYIFPSWWNGMPAIMKG
                     YVDRVFQHGFAYSFESDEPRKLFSTKKAIFFTPTGQPQNADGSLTPIDQAMKSLTSEW
                     LFNSNGAQVIDHIFYGRVPYLTREELELYLVDAREQIQRIFHKL"
     gene            2346..3605
                     /locus_tag="U1099_001658"
     CDS             2346..3605
                     /locus_tag="U1099_001658"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM4867968.1"
                     /translation="MRKIVINGGKTLKGSVTVSGAKNSVVALIPAIILADGIVTLDGV
                     PAISDVDSLVDIMETMGATVKRDGETLEIDPRGVKDMPMPFGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFAAMGAETTYEGDYMRLATNGQRIQGAHIFM
                     DVVSVGATINTILAAVKAEGRTVIENAAREPEIIDVATLLNNMGARIRGAGTDIITIE
                     GVDSLKGTRHQVIPDRIEAGTYIALAAAVGEGIRIDNVLYEHLESYIAKLEEMGVRMT
                     ISEDSIFVEKQEKLKAVSVKTSPYPGFATDLQQPITPLLLKAEGTGTIVDTIYEKRVG
                     HVQELANLGAKISTRNNHIAFEGPNQLTGTSVKATDLRAGAAMVIAGLMAQGRTEITN
                     IEFILRGYSNIIDKLTELGADIQLIED"
     gene            3700..4272
                     /locus_tag="U1099_001659"
     CDS             3700..4272
                     /locus_tag="U1099_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4867969.1"
                     /translation="MTLWTRLAKFAYFETDRLYLRPFSFEDGESFYEIVANPENLPFI
                     FPALEDRNIAFSTMVEKFMRSPLGNWALVDKYSERMIGALCFEKVDERQLSAELSYFL
                     KKDYWRRGLMTEAVRTLVYLAFYEFGLRELVIVTHEENVASQMVAKKAGLVQVAQYRG
                     SDRYSHKTRNYLKFSLKRAEFSLEMYEENE"
     gene            4269..5129
                     /locus_tag="U1099_001660"
     CDS             4269..5129
                     /locus_tag="U1099_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEM4867970.1"
                     /translation="MITIKSQREIDAMNRAGDVLAGIHIGLREIIKPGVDMWEVEEYV
                     RKTCKEKNVLPLQIGVDGELMDYPYATCCGLNDEVAHAFPRHYKLKEGDLLKVDMVLS
                     EPLDKAVVDVSKLNFNDVKAMKKITQTYRGGCADSCWAYAIGQVSEEVQNLMDVTKEC
                     LYRGIEQAVVGNRIGDIGAAIQEYAEGLGYGVVRDLVGHGVGPTFHEEPMVPHYGTKG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5177..6106
                     /locus_tag="U1099_001661"
     CDS             5177..6106
                     /locus_tag="U1099_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEM4867971.1"
                     /translation="MKKTKIVEGLKTFCSTFFSFYRSADSDVTSVAVAYYLLISIFPI
                     LLTLANLLPYYPFDVDMILSVVAEFVPDKLYPSVSSFITSVLTKPSSSWLGISILTTL
                     WTLSRSMTILQKAFNKAYGINEHRDFIIGHLIGIFLGIGLQVIILLSITLLTFGQAVF
                     SYINKLVPIEDAWLKGLLSQTQLVGLMALFAALVMLYFFLPNVRIKKVRYVFPGTLFV
                     LLTMTSIGKLFSIYVDNYANKLLDFRIVTAVVFLVFMLWFIFMAQVLIIGAMINATVQ
                     SMQVEEFHARDGDIVSILNRLKARFTAIDKEWE"
BASE COUNT         1710 a         1078 c         1500 g         1896 t
ORIGIN      
        1 tctttatttt taagaaaatg ctaggatagt cggtcaatct atgctatact agaaacgtta
       61 ttaagtcccg aaaaggtagt ttatagacta gttaatattt gcagaaatac ttgaaacaca
      121 attaaagaag ctggtaacat tgaatagtaa gcgtaaaaac tttactacac ttcagtcacc
      181 attttcttca aataaggctg atttcataca gtctggtatt gtctttctcg ttgagtatct
      241 ggaaatttcc ctttgcttaa gacaggtgtc tttcgcaaag aactagtctt cgactagttc
      301 tttgtaacct tagtaacccg ctaggtttct agcatatatt ctattttaag ggggacattt
      361 ttatgtcaga acgtaagttg tttacgtctg aatctgtttc ggagggtcat ccggataaga
      421 ttgcagacca gatttcagat gccattttgg acgctatttt agcagaggat ccagatgcgc
      481 acgtagcggc agagacggct gtttatacag gtagtgttca tgtgtttgga gagatttcaa
      541 ctactgccta tgtggatatt aaccgtgtgg tacgggatac cattgcggag attggctata
      601 caaaaggtga gtatggtttt tcagctgagt cggttggggt tcatccgtca cttgtagagc
      661 aatcgccaga tattgcccga ggcgtcaatg aagctttaga aactcgtcaa gatgctagtc
      721 aagatccatt ggatttgatt ggtgcaggtg accaggggct catgtttggt tttgcagtag
      781 atgaaacgcc tgaacttatg ccgttgccaa tttcacttag tcacaaattg gtgcgtcgtt
      841 tggccgagtt gagaaaatct ggtgaaattg cttatctccg tccagatgct aagtcacagg
      901 taacagttga atacgatgaa gacaatcaac ctgttcgtgt ggatacagtc gttatttcga
      961 cccagcatga tccagaagtt agccaggagc aaattcgtca ggatgtcatt gaacgtgtga
     1021 tcaaggaaat cattccagct cattatctgg atgaccaaac caactacttt atcaacccaa
     1081 ctggtcgctt tgtcattggc ggacctcaag gggactctgg tttgacaggc cgtaagatta
     1141 tcgttgatac ctatggtggt tattctcgtc atggcggcgg tgccttctct ggtaaggatg
     1201 cgaccaaagt ggaccgttct gcatcttatg cggcacgcta catcgcaaaa aatatcgtcg
     1261 cagctggttt ggctaaaaag gcggaagttc aattggctta cgctatcggt gttgctcacc
     1321 cagtttcagt tcgcatcgat acctttggta cgagcacagt agcggaaagc aaactagaag
     1381 cagctgtccg tcagattttt gacctgcggc cagcaggtat tatccagatg ctggacctca
     1441 atcgcccgat ttacagacaa actgcggcct atggccacat gggccggacc gacattgacc
     1501 taccttggga aaaattagac aaggtagagg cgttgaaaac tgcggttttg taatgaaata
     1561 gttgtccatt tagtgagaag tttatgaaag cgttaattgt ctttgcacac cctagaaaag
     1621 aaagctttac acatgctttg gttgatagag tggcggaagc tctgctggaa aatggagcag
     1681 aagttgtcat ccgagattta tacgaaatag gattcgatcc cgtgttacgt ggagaagata
     1741 ccattcatat tgaagatggc aaatttatac gtcaggcaac ggtttttcca gcagatgttc
     1801 aagttgaaat ggatttgatt gccgaaagcg acttactagt ctacatattt ccgagttggt
     1861 ggaatggaat gcctgctatt atgaaagggt acgtcgaccg tgtatttcaa catggctttg
     1921 cttacagttt tgaatctgat gaaccacgta aattattttc cactaagaaa gctattttct
     1981 ttacgccaac agggcagcca caaaatgcag atggaagtct gacaccgatt gatcaagcga
     2041 tgaaaagctt gacctcagaa tggctcttca atagcaacgg tgctcaagtc attgaccata
     2101 tcttctatgg gcgagtacct tatttaacga gagaagagct ggagttgtat ctcgtggatg
     2161 caagggagca aattcagaga atttttcata aattatgaag agagattcac taatagtatg
     2221 taaatatgaa ggactgagct cattggctcg gtccttttgc atgctatata actagctttt
     2281 tccaagactt tatgatagaa tgaaacgtat gcctttttag gcaaattaag aaagtgaatg
     2341 ttagtatgag aaaaattgta attaatggtg gaaaaacact gaaagggtcg gttaccgtta
     2401 gtggtgcaaa aaactcagtt gttgctctta ttccggccat tattcttgca gacgggattg
     2461 ttactctgga cggagtacct gcaatctcgg atgtagatag cttagttgat attatggaaa
     2521 caatgggcgc gacggtcaag cgtgatggtg aaaccttgga gattgaccct cgtggtgtaa
     2581 aagacatgcc tatgccattt ggcaaaatta atagcttgcg tgcatcctac tatttttacg
     2641 gttccctcct tggtcgcttt ggtcaagctg ttgttggctt gccaggtggt tgtgatttgg
     2701 gaccgcgtcc gattgatttg cacttgaaag cattcgcggc aatgggagct gagacaactt
     2761 atgaaggtga ctatatgcgc ttggcaacta atggtcagcg tattcaaggg gcccatatct
     2821 tcatggatgt tgtcagcgtc ggtgcgacaa ttaatactat cttagcagct gtcaaagcag
     2881 aaggccgtac agttattgaa aatgcggcgc gtgagcctga aattattgat gtggcaacgt
     2941 tgcttaataa tatgggggca cgtatccgtg gtgcgggtac agatattatc accattgaag
     3001 gcgtagacag tctaaaagga acacgtcatc aagtgattcc agaccgtatt gaagcaggga
     3061 cttatattgc ccttgctgct gctgttggtg aaggtattcg tatcgataat gtcctttatg
     3121 aacacttaga gagttacatc gctaagttgg aagaaatggg tgttcgcatg accatttctg
     3181 aagatagtat ttttgttgaa aagcaagaga aattgaaggc ggtaagtgtg aaaacttcac
     3241 cttatccagg ttttgcgaca gacttgcagc aacccattac acctttattg cttaaggcag
     3301 aagggactgg tacgattgtt gatacgattt atgaaaaacg tgttggtcat gtgcaggagc
     3361 ttgctaatct tggagcaaaa atctctactc gtaacaacca tattgccttt gaaggtccaa
     3421 atcaactgac tggtacatct gttaaagcta ccgatttgcg tgcgggagcc gcaatggtta
     3481 ttgctggttt aatggctcaa gggcgtacag aaattacaaa tattgagttt attctacgtg
     3541 gttactcaaa tattattgat aaactgacag aattgggtgc agatattcag ttgattgagg
     3601 attagagagt ctaattgaga actactttct caaaacgtaa acgatttaaa aacaaaaaaa
     3661 tctggggctg ggagtaattc tcagcccctt gggtacacta tgacactttg gacaagacta
     3721 gcgaagtttg cttactttga aacggaccgt ttgtatttac gaccattttc ttttgaagat
     3781 ggagaaagtt tctatgaaat tgtagcaaat cccgaaaatc ttccttttat ctttccagct
     3841 ttggaggaca gaaatattgc tttttcgaca atggttgaaa aatttatgcg ttcaccattg
     3901 ggaaactggg ccttagttga taagtattcg gagaggatga ttggtgcgct ttgctttgaa
     3961 aaggtagatg aacgtcaact ttctgcggag ctcagctatt tcctaaaaaa agactactgg
     4021 agaagaggct taatgacgga agcagtgagg actttagtct atctagcttt ttatgagttt
     4081 ggtctgaggg agctggttat tgtcactcat gaggaaaatg tggccagtca gatggtggcg
     4141 aaaaaagctg gtttggtaca ggttgcgcag tatcgtggaa gtgaccgcta tagccataaa
     4201 acaagaaatt atctaaaatt tagcttgaag agggctgaat ttagtttaga aatgtatgag
     4261 gagaatgaat gattaccatt aaatcacaac gagaaattga tgcaatgaac cgtgctggag
     4321 atgttctggc tggtattcat attggtttgc gagagattat caagccaggt gtggacatgt
     4381 gggaagtaga ggaatatgtc cgtaaaacgt gtaaggaaaa gaatgttttg ccactccaaa
     4441 ttggtgttga tggagaattg atggattatc cttatgcgac ttgttgtgga ttgaatgacg
     4501 aggtcgctca tgcctttccg cgtcattaca aacttaaaga aggtgattta ctgaaggtcg
     4561 atatggtttt gagcgagcct ttggataaag cggtagttga tgtttctaag ctcaacttta
     4621 atgatgtcaa ggctatgaag aaaatcaccc agacctatcg tggtggttgt gcggattcct
     4681 gctgggctta tgcgattggt caggtctctg aggaagttca aaacttgatg gacgtgacta
     4741 aggaatgttt gtaccgcggt atcgagcaag cagttgttgg caatcgtatc ggcgatatcg
     4801 gtgcagccat tcaagaatat gcagaaggtt tgggctacgg tgttgttcgt gacttggtgg
     4861 gtcacggcgt tggaccaacc ttccacgaag agccgatggt tcctcattat ggaactaaag
     4921 gtcgtggtct tcgcttgcgt gagggcatgg ttttgactat tgagccaatg attaatacag
     4981 ggacttggga aattgatacg gatatgaaga ctggttgggc tcataagaca cttgatggtg
     5041 gcttgtcttg tcaatacgaa caccaatttg tcatcaccaa agatggaccg gttattttga
     5101 ccagtcaagg tgaagaaggg acctattaat tctcggtgtg gtcgtgaata atagatgaat
     5161 gaggaaggag gagagaatga agaaaaccaa aatagtcgaa ggtttgaaaa cattctgctc
     5221 aaccttcttt tctttttatc gttctgctga tagcgatgta acaagcgttg cagtagctta
     5281 ctatttatta atttctattt tccctattct tctaactctt gcgaatttac tgccatatta
     5341 tccatttgat gtggatatga ttttatcggt tgtagctgaa tttgtgccag acaagctcta
     5401 tccttcggtt tcatctttta tcacttctgt attaacaaag ccgtcgtcat cgtggttagg
     5461 gatttcaatt ctgacaactc tttggaccct gtcacgtagt atgactattt tgcagaaggc
     5521 ctttaataag gcttatggta tcaatgaaca ccgtgatttt attattgggc acttgattgg
     5581 catattttta ggaattggtc tgcaggtaat tatcctctta agtattacgc ttttgacttt
     5641 cgggcaggca gttttttctt atattaataa gctggttcct attgaagacg cctggctgaa
     5701 gggacttctt agtcaaacac agttagtagg tttaatggct ctgtttgccg ccttggttat
     5761 gctttatttc tttttgccaa atgttcggat aaaaaaagtt cgatatgttt ttccaggaac
     5821 gttgtttgtt ctgttgacga tgacttctat tggaaagtta ttttcgattt atgttgataa
     5881 ttatgctaat aagttgctgg attttagaat agtaacagct gtcgtctttc ttgtttttat
     5941 gctttggttc atttttatgg ctcaggtact catcattggt gcaatgatta atgctacggt
     6001 tcaaagtatg caggtagaag agtttcatgc cagagatggg gatattgttt caatcttgaa
     6061 taggctaaag gcaagattta cagcgataga taaagaatgg gagtgagaaa gaactctatt
     6121 ttcaaaaatg agttcgtctt ctcacccccg cacagttgat taggccagat ttggagtgtg
     6181 aaac
//
LOCUS       DASFSW010000113         6177 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.113, whole genome shotgun sequence.
ACCESSION   DASFSW010000113 DASFSW010000000
VERSION     DASFSW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.113"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..909
                     /locus_tag="U1099_001662"
     CDS             <1..909
                     /locus_tag="U1099_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEM4867972.1"
                     /translation="GALVEAFGGEVIHVSAKSKDFLNPLDINFNYGDGDAPLKDKANF
                     IMSMLELVVGGSGLTAAEKSVIDRCLPKIYEAYFENPIPENMPILQDLYDLLLKQEES
                     VGRKLATEMEIYVTGSLNVFNHRSNVDLNKSLLCFDIKELGTQLKKIGMLVIQDQVWN
                     KVSQNRQSQKSTRYYIDEFHLLLKEEQTAQYSVEIWKRFRKWGGIPTGLTQNVKDLLA
                     SKEIENVFDNTDFIMMLNQASGDREILARKLKISPYQLNYVTNSNAGEGLLFFGNTIV
                     PFLDQFPKNTLLYQKMTTKPEEVRKG"
     gene            2900..4138
                     /locus_tag="U1099_001663"
     CDS             2900..4138
                     /locus_tag="U1099_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEM4867973.1"
                     /translation="MKEDKIFQRQSFYRKSMYRQRMKQQIRNKHFPRIRDRIKEELKK
                     LLNQMVQFITTKWKSILVLALALFSLLTVVYSNSQFMFGALSSVSYQLTTTSYLSSEE
                     MLVALNHIFSTYELNLSNQLARLKESKPGYDEYIVKGREQIGHDPHVLLAYLTARFGE
                     IKDQTSVEPAMKQLFVQIYQVSYREEREIRYRKVKEEVLDTSGKKTIVEKEVPYTYRK
                     LIATVSKNDMDVVIQSIFAGSPSNLEHYRILRETKGNLETAFPSGTTISLPGNVSSVY
                     DVNLTGGNFPPPNPNHVAALNGGYPGQCTWYVYNRFSQLGKPIQHSPMGNGGEWAFYA
                     AKYGYPVSREARAGTAVCMPPTVPYADPTYGHIAFVERVNPDGSIVISEMNVKGEFVI
                     STGYLPREMAAQCYYINFGL"
     gene            4149..4364
                     /locus_tag="U1099_001664"
     CDS             4149..4364
                     /locus_tag="U1099_001664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867974.1"
                     /translation="MRKDIQMLQKKKEKLLEKKQKIDQDLAQVLQEEQDLIYEEVIVV
                     FEKSNLSLEEFTKKVLFEKELSHETII"
     gene            4348..4944
                     /locus_tag="U1099_001665"
     CDS             4348..4944
                     /locus_tag="U1099_001665"
                     /inference="COORDINATES: protein motif:HMM:NF025641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HEM4867975.1"
                     /translation="MKRLFSFVRVASLVMLFSGCQTIYASSPSSEPNSQPVAISVHPQ
                     ASEQSNAKGQVTENVDANNQDYEITDSVATKRQFVTFTSKSGKVFHLIIDHDKGGQNV
                     QLLTEVSEQDLLNLVESTDTVAVKPQKVEEVVDDKPVKNEEPKQKSSAGSYIIIGLFL
                     LGVLCVGYYIKVIKPKKEHHFEEFEEDDDYISDGEEEV"
     gene            4987..5625
                     /locus_tag="U1099_001666"
     CDS             4987..5625
                     /locus_tag="U1099_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HEM4867976.1"
                     /translation="MDLSSKQIKNLSHNKWYHATLKRHLSSLLEGIKVDYNLGHELDF
                     GAGFYITSDFQQAQKYINRLVEFLNTNKAGAEFLGNMDEEGIILEFELTDFPSIFTTS
                     NYKCHYFKSHNISDDVIDFAEFVFQNRLRSKERIHDFDFIYGVQTDDNPTGLLQKYKA
                     GLVSKADVLNEFRKPFSFKQLSIHNQEFCDIMKVTKIYLSKDGKELDQWQKQ"
     gene            5610..5840
                     /locus_tag="U1099_001667"
     CDS             5610..5840
                     /locus_tag="U1099_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867977.1"
                     /translation="MAKTMISPVELYSNELAQALLETSKYRLEASVAHQIARQYASQV
                     DFEDPILMHVGVNSIASTLIDKIKPEYFQTTS"
     gene            5917..>6177
                     /locus_tag="U1099_001668"
     CDS             5917..>6177
                     /locus_tag="U1099_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM4867978.1"
                     /translation="MYFFDLFAGIGGFRLGLEQAGHICVGFCEIDRFARNSYQAIFDT
                     EGEVEYHDIRKISNHAFRKLRGKVDLICGGFPCQAFSLAGRRL"
BASE COUNT         2227 a          981 c         1194 g         1775 t
ORIGIN      
        1 ggtgctcttg tagaagcatt tggtggagag gttatccatg tttccgctaa atctaaggat
       61 tttttgaatc cattagatat aaacttcaac tatggagatg gcgatgcacc tctgaaagat
      121 aaggcgaatt ttatcatgtc aatgctcgaa cttgtagtag gcggttctgg gttaacagcc
      181 gctgaaaaat ctgtcattga ccgatgtcta ccgaaaatct acgaagcata cttcgaaaac
      241 ccaattccag agaatatgcc aatcttacag gatttatatg atttgttgtt aaaacaggag
      301 gaaagcgttg gtcggaaatt agcgactgaa atggagatat atgtaacagg ttctctaaat
      361 gtatttaatc atcgttcaaa tgttgacttg aataaatcac ttctatgctt cgatataaaa
      421 gagttgggga cacaattaaa aaagattggt atgttagtta ttcaagacca ggtatggaac
      481 aaggtttcac aaaataggca aagtcaaaaa tctacacggt attatattga tgaatttcac
      541 ttactgctaa aggaggaaca aacagctcaa tattcggttg aaatttggaa gaggtttcgt
      601 aagtggggag ggattccgac aggtttaact caaaacgtaa aagacctttt agcgagtaag
      661 gaaatagaaa atgtctttga taatacggac tttatcatga tgttgaacca ggcttcaggg
      721 gacagagaaa ttctagctag aaaactaaaa atatctccct atcagctcaa ttatgtaacc
      781 aactctaatg ctggagaggg tctactattc tttggaaata ccattgttcc cttcttagac
      841 caattcccta agaatacact cttgtatcaa aagatgacaa ccaaaccaga agaggtcagg
      901 aagggctagg tgaattatgg gaaagtcaaa acgaatggtc cgagagacca ataagaagca
      961 ttatacaaaa tcaaaacctg acattagtca acggaaggaa gagcaagatt tttctggaag
     1021 aacagttgat gagagtgtta aatttcaaca ccagattatt cataaagaaa gtaaattaga
     1081 gagcaggaaa aatcaagttg aatcttctgg aaagaggcgg aaaaatatta atattgtgga
     1141 tgacaagtct acacaaactc ctttacctat tcatgataaa aatgaatcaa ctattcaaat
     1201 tacagatcta caagatgaaa tagttaataa aaattattta catcagccct ctccaccaga
     1261 agcaccccta gttgaatcaa agccaataag tcataagaaa caagtccaat tacaacaacg
     1321 gaaagccttt gaaaatcagc aaaatgttga atcaaggaac gatattgagt tcaccgagca
     1381 gaatggccat aattctgaaa tacacgttag ttctgatggg gatcttgcac aaactggtaa
     1441 tgaacaagaa gttcaacaaa tacaatttga aattgtgcac cataatccag taaattccag
     1501 cgaactaaat cactcaaagg aattgaattt cggaccaact tcaagtcaaa ctcaggcaca
     1561 aaataattct caaccgactg aatctattga tagaaaaatt catgatactc cgtttcgtaa
     1621 gtttgataca aatcaaagta gaatcattga tactgaaaca aagtttaccc atcggagaaa
     1681 tgaggggaga gcaaaattaa ttcacaatca gaatcaagga acgccgtccc agtttaagaa
     1741 taaaaagggg aagaaacatg ttcatggcga ttcagttcat gaaagtcaaa acattctttc
     1801 ggaattacct atttctcaat ttgattcttc tacagattca gttccaataa aaacttatcc
     1861 aagagtgagt gatgagtcta attctattat tgatcaagca gataatgaga gtgtctttga
     1921 tgtttcactt ctacaaagtc aattgagaaa agataataag aaatttcaac cgagtaattc
     1981 taaactttct aaaaaatcag gtgtcaaaat aatagaggca aaaaatagcc aaagtcaaca
     2041 aattgaaaaa actgtaactc ttacagatgg gggtaaacaa agttcaaacc aatttcagtc
     2101 aaatcttaat gaaaattcga taatgattga acagataagt tatcccaaca tcgactggtc
     2161 taaaaaactt catctacctc aaattaaact attctctaga gatattttgc actctaatgc
     2221 tgtaagcggt aagattcaga atgatgagag taaaccattg accagaaaag gtaagagatt
     2281 atcaaataga ctgagcaata aagcaacaaa atcgaatatt gctcgtagtt tatatcctga
     2341 tacaactagt gacaaaaata gaaaaactgt atctaaatca tccaaaacga aattgagtat
     2401 cactccaatt caagttgatg gaagaccaaa atattatttt gaaactacaa caaaaaaaca
     2461 attttataaa gggcgacctt cttcaaccaa caacattaag gcgaataaat ttagacgtca
     2521 aagattatta aaagatcagt tacaaattca tgaaaagttg aattccttag taattgagcc
     2581 aatagagttt gtaaaggata agaagaatcg tcgtttgctt aaaaaaattg aacaagcaaa
     2641 acttggcaga ataaaatcaa gcccacttaa aaaagtagtg tcaggattgg ccagcagtga
     2701 gtttctagca gcaagttatc ttcaagcagg agctgatgat aatgtggcag ttgatgcaac
     2761 ttcgaaagta ttgaacatca atgctgctat actaatgaga cttgcaaaaa aatcgaatcg
     2821 taatcagacg ataaagaaat tgactaagaa ggctaaggtt agacaatcgc aattggaatt
     2881 tcggcataag tatcgattga tgaaggaaga taagattttt caacggcaat cattctatcg
     2941 aaagtcaatg tatcgacagc ggatgaagca acaaattcgt aacaagcact tccctagaat
     3001 tagagacagg ataaaggaag aactaaaaaa actgttaaat caaatggtac agttcattac
     3061 cacaaagtgg aagagtattt tggtattggc tttggcttta ttctctctat tgacagttgt
     3121 atattcaaat agtcaattta tgtttggagc tttatcatca gtttcttatc agcttaccac
     3181 tacatcctat ctttcttcag aggaaatgct tgttgcattg aatcacatat tctctaccta
     3241 tgaattgaat ttatccaatc aattggcaag attgaaggaa tcgaaacctg gatacgatga
     3301 atacatcgtt aaagggaggg aacaaattgg tcatgatcca catgtcttat tagcctattt
     3361 gacagctaga tttggggaaa taaaagacca aacaagtgtt gaaccagcga tgaaacaact
     3421 atttgttcag atatatcagg tttcctatcg tgaagagagg gaaatccgtt atcgaaaggt
     3481 caaggaagaa gttcttgaca ctagcggtaa gaaaacgatt gttgagaaag aagttcccta
     3541 tacctatcga aaattgatag caacagtttc aaaaaatgac atggatgtag ttattcagtc
     3601 aatatttgcc ggcagtccta gcaatttaga acattatagg attcttaggg aaacaaaagg
     3661 gaatttagaa actgcctttc cttcgggaac gacgatcagt cttcctggaa atgtatcatc
     3721 ggtatatgat gtcaatttaa caggcggaaa ttttccacca ccaaacccta atcatgttgc
     3781 tgctctaaat ggcggttacc ctgggcaatg tacttggtat gtttataatc gattctcaca
     3841 acttggaaag ccaatccaac attcaccaat gggaaatggt ggcgagtggg cgttctatgc
     3901 tgctaaatac ggttatccag tcagtagaga ggcacgagct ggtacagcag tctgtatgcc
     3961 tccaactgta ccatatgcag atccgactta cggtcacatt gcgtttgtag agcgcgttaa
     4021 ccctgatggt agtattgtta tctcagaaat gaatgtaaaa ggggaatttg tgatttcaac
     4081 aggctatcta ccacgtgaga tggcggcaca atgttattac attaattttg gattataagg
     4141 agaaatatat gcgtaaagat attcaaatgt tacaaaaaaa gaaagaaaaa ttacttgaaa
     4201 aaaagcaaaa aattgaccaa gatttggctc aagttttaca agaagaacaa gatttgattt
     4261 atgaagaagt catagttgtt tttgagaaat ctaatctatc cctcgaagaa tttactaaaa
     4321 aagttttatt tgaaaaggag ttgtcgcatg aaacgattat ttagttttgt gagagtagct
     4381 agtttggtta tgttatttag cgggtgccaa actatttatg caagttcacc tagttcagag
     4441 ccaaatagtc agccagtagc aatttctgta catccacaag cctcagagca aagcaacgcc
     4501 aagggccaag tcacagaaaa tgtagatgcc aataatcaag attatgaaat tactgattca
     4561 gttgctacta agcggcagtt tgtcactttt acaagtaagt caggaaaggt gtttcacctg
     4621 attatagatc atgacaaagg tggacaaaat gtacaattat tgacagaagt atctgaacag
     4681 gatttactta accttgttga atcaactgat actgttgcag tcaaaccaca aaaggttgaa
     4741 gaagtcgttg atgataaacc tgtaaaaaat gaagaaccaa aacaaaaatc ttctgctgga
     4801 agttacatta ttatcggtct atttctcctt ggtgttttgt gtgtaggata ttatataaaa
     4861 gtcattaaac caaagaaaga acaccacttt gaagaatttg aagaagatga tgactatata
     4921 agtgatggtg aagaagaggt ttgacattgt tttccttttt tgtataatga aattagaggt
     4981 aatgaaatgg atttatcaag taagcaaatt aagaatttat cacataataa atggtaccat
     5041 gctacattaa aacgtcatct ctcttcctta ttagaaggga taaaagtaga ctacaattta
     5101 ggacatgaac tggattttgg tgctggtttt tatattacat cagattttca acaggcacag
     5161 aagtatataa accgtttggt agaattttta aataccaata aagctggtgc tgaatttttg
     5221 gggaatatgg atgaggaagg gattattcta gagtttgagt tgacagattt tccttcaatt
     5281 tttacaacat caaattataa gtgtcattat ttcaaaagcc acaatatttc tgatgatgtg
     5341 atagatttcg ctgagttcgt ttttcagaat cggcttcgct caaaggaacg cattcatgat
     5401 ttcgatttta tttatggagt tcagacggat gacaatccaa ctggtttact ccaaaaatat
     5461 aaggctggat tggtttcaaa agctgatgta ttgaacgaat ttcgtaagcc gttctctttt
     5521 aaacagttat ccattcacaa tcaagagttt tgtgatataa tgaaggtaac aaagatttat
     5581 ttatcaaaag atggaaagga gttagaccaa tggcaaaaac aatgattagt cctgttgaat
     5641 tgtatagcaa tgagttagca caggctttgt tagaaacatc aaaatataga cttgaggcta
     5701 gtgttgcgca tcagatagca agacaatatg caagtcaggt agattttgaa gatccaattt
     5761 tgatgcacgt tggagttaac tccattgcaa gcaccttgat tgacaaaatt aaacctgaat
     5821 attttcaaac aacttcataa agtttttaaa cttgaatgat gattttagcg attagagaaa
     5881 tctagtcgct tttttctttg tcaaaaagga ggataaatgt atttttttga tttatttgct
     5941 gggataggag gttttcgatt aggtcttgaa caagctggac atatttgtgt tggcttttgt
     6001 gagatagaca gattcgctcg taactcctat caggctattt ttgatacaga aggagaagtc
     6061 gaatatcatg acatacgaaa aatatccaat catgctttcc gaaagctccg aggaaaagtt
     6121 gatctcatct gtggtggatt cccttgccag gcattttcgc ttgcaggaag aagattg
//
LOCUS       DASFSW010000114         6172 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.114, whole genome shotgun sequence.
ACCESSION   DASFSW010000114 DASFSW010000000
VERSION     DASFSW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.114"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            174..1722
                     /locus_tag="U1099_001669"
     rRNA            174..1722
                     /locus_tag="U1099_001669"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1776..1848
                     /locus_tag="U1099_001670"
     tRNA            1776..1848
                     /locus_tag="U1099_001670"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1809..1811,aa:Ala,seq:tgc)
     gene            2151..5054
                     /locus_tag="U1099_001671"
     rRNA            2151..5054
                     /locus_tag="U1099_001671"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5243..5358
                     /gene="rrf"
                     /locus_tag="U1099_001672"
     rRNA            5243..5358
                     /gene="rrf"
                     /locus_tag="U1099_001672"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5364..5436
                     /locus_tag="U1099_001673"
     tRNA            5364..5436
                     /locus_tag="U1099_001673"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5397..5399,aa:Val,seq:tac)
     gene            5439..5511
                     /locus_tag="U1099_001674"
     tRNA            5439..5511
                     /locus_tag="U1099_001674"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5472..5474,aa:Asp,seq:gtc)
     gene            5557..5629
                     /locus_tag="U1099_001675"
     tRNA            5557..5629
                     /locus_tag="U1099_001675"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5590..5592,aa:Lys,seq:ttt)
     gene            5633..5714
                     /locus_tag="U1099_001676"
     tRNA            5633..5714
                     /locus_tag="U1099_001676"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5667..5669,aa:Leu,seq:tag)
     gene            5729..5801
                     /locus_tag="U1099_001677"
     tRNA            5729..5801
                     /locus_tag="U1099_001677"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5762..5764,aa:Thr,seq:tgt)
     gene            5814..5885
                     /locus_tag="U1099_001678"
     tRNA            5814..5885
                     /locus_tag="U1099_001678"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5846..5848,aa:Gly,seq:gcc)
     gene            5893..5976
                     /locus_tag="U1099_001679"
     tRNA            5893..5976
                     /locus_tag="U1099_001679"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5927..5929,aa:Leu,seq:taa)
     gene            5995..6068
                     /locus_tag="U1099_001680"
     tRNA            5995..6068
                     /locus_tag="U1099_001680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6029..6031,aa:Arg,seq:acg)
BASE COUNT         1707 a         1276 c         1770 g         1419 t
ORIGIN      
        1 caagaaatga taaactaaga tagttgtcgc gagaacgtga taacgacaag acctttgaaa
       61 attaaagaag acgaaccaaa cgtgcagggt gatttatcta aggataaatc gtcaatgaca
      121 aaacaaaaca ataaaacgga aagctagcaa tagcttgagt ttgaatcaaa actttttatg
      181 agagtttgat cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtagaac
      241 gctgaagtct ggtgcttgca ctagacggat gagttgcgaa cgggtgagta acgcgtaggt
      301 aacctgcctc atagcggggg ataactattg gaaacgatag ctaataccgc ataacagtat
      361 ttaccgcatg gtagatgctt gaaaggagca actgcttcac tatgagatgg acctgcgttg
      421 tattagctag ttggtgaggt aacggctcac caaggcttcg atacatagcc gacctgagag
      481 ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg
      541 gaatcttcgg caatgggggc aaccctgacc gagcaacgcc gcgtgagtga agaaggtttt
      601 cggatcgtaa agctctgttg taagagaaga actgtgagaa gagtggaaag tttctcactt
      661 gacggtatct taccagaaag ggacggctaa ctacgtgcca gcagccgcgg taatacgtag
      721 gtcccgagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tgataagtct
      781 gaagtaaaag gctgtggctt aaccatagta cgctttggaa actgtcaaac ttgagtgcag
      841 aaggggagag tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacaccg
      901 gtggcgaaag cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcga
      961 acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaggt gttgggtcct
     1021 ttccgggact cagtgccgca gctaacgcat taagcactcc gcctggggag tacgaccgca
     1081 aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat
     1141 tcgaagcaac gcgaagaacc ttaccaggtc ttgacatccc gatgaccgcc ctagagatgg
     1201 ggtttctctt cggagcatcg gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga
     1261 gatgttgggt taagtcccgc aacgagcgca acccctattg ttagttgcca tcattcagtt
     1321 gggcactcta gcgagactgc cggtaataaa ccggaggaag gtggggatga cgtcaaatca
     1381 tcatgcccct tatgacctgg gctacacacg tgctacaatg gctggtacaa cgagtcgcaa
     1441 gtcggtgacg gcaagctaat ctcttaaagc cagtctcagt tcggattgta ggctgcaact
     1501 cgcctacatg aagtcggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt
     1561 cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg
     1621 aggtaacctt ttaggagcca gccgcctaag gtgggataga tgattggggt gaagtcgtaa
     1681 caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggaa atggaaacct
     1741 gtacgtcagt cttctttaat tttgagaggt cttgtggggc cttagctcag ctgggagagc
     1801 gcctgctttg cacgcaggag gtcagcggtt cgatcccgct aggctccatt aggatagaaa
     1861 tcctactaaa ctgaataaca gtgaagttga attcacgact aacttcttag gaaaatagat
     1921 aatcttcctt gtgtgcaaga cacacaatgt cagattccta attttctaca gaagtttcgc
     1981 agaagcgacc gtcgttcata tcctaaactt gtccattgaa aattgaatat ctatcaaaca
     2041 ttcctaaacg tatgtaaaaa tacgtataga aatagtaaca agaaaataaa ccgaaaacgc
     2101 tgtgaatatt taatgagttt tctaattttt gaaaaaaatt aggttaataa ggttaagtta
     2161 ataagggcgc acggtggatg ccttggcact agaagccgat gaaggacgtg actaacgacg
     2221 aaatgccttg gggagctgta agtaagcaat gatccaggga tgtccgaatg ggggaacccg
     2281 gcaggtaatg cctgtcactc actactgtta aggtagtgaa gaggaagacg cagtgaactg
     2341 aaacatctaa gtagctgcag gaagagaaag caaaagcgat tgccttagta gcggcgagcg
     2401 aaacggcagg agggcaaacc gaggagttta ctcctcgggg ttgtaggact gcaatgtgga
     2461 cttaaagagt atagaagaac tacctgggaa ggtaggccaa agagagtaat agcctcgtat
     2521 ttgaaatatt ctttatacct agcagtatcc tgagtacggc gagacacgcg aaatctcgtc
     2581 ggaatccggg aggaccatct cccaacccta aatactctct agtgaccgat agtgaaccag
     2641 taccgtgagg gaaaggtgaa aagtaccccg gaaggggagt gaaatagaac ctgaaaccgt
     2701 gtgcctacaa caagttcgag cccgttaatg ggtgagagcg tgccttttgt agaatgaacc
     2761 ggcgagttac gatatgatgc gaggttaagt tgaagagacg gagccgtagg gaaaccgagt
     2821 cttaataggg cggattagta ttatgtcgta gacccgaaac catgtgacct acccatgagc
     2881 aggttgaagg tgcggtaaga cgcactggag gaccgaacca gggcacgttg aaaagtgctt
     2941 ggatgacttg tgggtagcgg agaaattcca aacgaacttg gagatagctg gttctctccg
     3001 aaatagcttt agggctagcg tcgacatttg agaatcttgg aggtagagca ctgtttggat
     3061 gagggggcca tctcggttta ctgatttcag ataaactccg aatgccaatg atttatggtc
     3121 ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg gaaacagccc agaccaccag
     3181 ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg ggttgcacag acaactagga
     3241 tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa tagctcacta gtcgagtgac
     3301 cctgcgccga aaatgtaccg gggctaaaac aatttaccga agctgtggat aacactttag
     3361 tgttatggta ggagagcgtt ctatgtgtga agaaggtata ccgtgaggag tgctggaacg
     3421 catagaagtg agaatgccgg tatgagtagc gaaagatggg tgagaatccc atccaccgta
     3481 agactaaggt ttccagggga aggctcgtcc gccctgggtt agtcgggacc taaggagaga
     3541 ccgaagggtg tatccgatgg acaacaggtt gatattcctg tactagagta tgaagtgatg
     3601 gagggacgca gtaggctaac taaagcgggc gattggaagt gcccgtctaa gcagtgaggt
     3661 gtgatatgag tcaaatgctt gtatctgtaa cattgagctg tgatggggag tgaagttaag
     3721 tagcgaagtt agtgacgtca cactgccgag aaaagcttct agcgatgtat catactctac
     3781 ccgtaccgca aaccgacaca ggtagtcgag gcgagtagcc tcaggtgagc gagagaactc
     3841 tcgttaagga actcggcaaa atgaccccgt aacttcggga gaaggggtgc tggcttaaag
     3901 tcagccgcag tgaataggcc caagcaactg tttatcaaaa acacagctct ctgctaaatc
     3961 gtaagatgat gtataggggg tgacgcctgc ccggtgctgg aaggttaaga ggagggttta
     4021 gcgcaagcga agatctgaat tgaagcccca gtaaacggcg gccgtaacta taacggtcct
     4081 aaggtagcga aattccttgt cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg
     4141 gcactgtctc aacgagagac tcggtgaaat tttagtacct gtgaagatgc aggttacccg
     4201 cgacaggacg gaaagacccc atggagcttt actgcagttt gatattgagt atctgtacca
     4261 catgtacagg ataggtagga gcctacgaag tcgggacgcc agtttcgacg gaggcgctgt
     4321 tgggatacta cccttgtgtt atggctactc taacccggat aggttatccc tatcggagac
     4381 agtgtctgac gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
     4441 aaggttccct cagaatggtt ggaaatcatt cgcagagtgt aaaggtataa gggagcttga
     4501 ctgcgagagc tacaactcga gcagggacga aagtcgggct tagtgatccg gtggttccgt
     4561 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
     4621 caagagttca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
     4681 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
     4741 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc
     4801 tgctcctagt acgagaggac cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa
     4861 aggcatcgct gggtagctat gtagggacgg gataaacgct gaaagcatct aagtgtgaaa
     4921 cccacctcaa gatgagattt cccataactt tatgttagta agagccctga gagatgatca
     4981 ggtagatagg ttggaagtgg aagtgtggcg acacatgtag cggaccaata ctaatcgctc
     5041 gaggacttat ccaaactaag taacgaaggc gtcaaatccg cctgaaaaat aggaacctaa
     5101 cgcagtatgc ttgcatacaa ggcaggttat cttttttcca aggatttagc ccgagtacaa
     5161 ttactcacga gtcaatattg acaagcgatc ggtttcttgt tagaatatag atattcaatt
     5221 ttgagttgac aagactcatt agttaagtga cgatagccta ggagatacac ctgtacccat
     5281 gccgaacaca gcagttaagc cctagaacgc ctgaagtagt tgggggttgc cccctgttag
     5341 atacggtagt cgcttagcaa attgggagtt tagctcagct gggagagcat ctgccttaca
     5401 agcagagggt cagcggttcg atcccgttaa ctcccatagg tcccgtagtg tagcggttat
     5461 cacgtcgccc tgtcacggcg aagatcgcgg gttcgattcc cgtcgggacc gttgaaacga
     5521 atcaatcata tgtttcaagt aatataatta agtaaagact cgttagctca gttggtagag
     5581 caattgactt ttaatcaatg ggtcgctggt tcgagcccag cacgagtcat atgcgggttt
     5641 ggcggaattg gcagacgcac cagatttagg atctggcgct ttcgggcgtg ggggttcaag
     5701 tcccttaacc cgcatagaat agaaataggc cggcttagct cagttggtag agcatctgat
     5761 ttgtaatcag agggtcgcgt gttcaagtca tgtagccggc attaggaaag attgcgaacg
     5821 tagttcagtg gtagaacacc accttgccaa ggtgggggtc gcgggttcga atcccgtcgt
     5881 tcgctttgag aggccggggt ggcggaactg gcagacgcac aggacttaaa atcctgcgat
     5941 ggcaacatcg taccggttcg attccggtcc tcggcatgaa gattataata gttagcaccc
     6001 ttagctcaac tggatagagt acctgactac gaatcaggcg gttagaggtt cgaatcctct
     6061 agggtgcatc actcgcttaa tggagacgtt aggggagctt tatttttaat agtatcggga
     6121 agtagctcag cttggtagag tacttggttt gggaccaagg tgtcgcaggt tc
//
LOCUS       DASFSW010000115         6138 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.115, whole genome shotgun sequence.
ACCESSION   DASFSW010000115 DASFSW010000000
VERSION     DASFSW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.115"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(86..2167)
                     /gene="fusA"
                     /locus_tag="U1099_001681"
     CDS             complement(86..2167)
                     /gene="fusA"
                     /locus_tag="U1099_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM4867979.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHERLQANAH
                     PIQLPIGSEDEFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLEQAEEYREKLVEAV
                     AETDEELMMKYLEGEEITNEELKAAIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGVNPDTDAEEERHASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESIHVPEPVIQLMVEPKSKADQDKMGIALSKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPIVDVKAKLY
                     DGSYHDVDSSETAFKVAASLALKEAAKSAQPTILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     DEIIKKNGGNA"
     gene            complement(2609..3079)
                     /gene="rpsG"
                     /locus_tag="U1099_001682"
     CDS             complement(2609..3079)
                     /gene="rpsG"
                     /locus_tag="U1099_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEM4867980.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKLVTRLINRVMLDGKRGTAASIVY
                     GAFDQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARNRGEHTMIDRLAKEIMDAANNTGAAVKKREDTHKMAEANRAFAHFRW"
     gene            complement(3096..3509)
                     /gene="rpsL"
                     /locus_tag="U1099_001683"
     CDS             complement(3096..3509)
                     /gene="rpsL"
                     /locus_tag="U1099_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEM4867981.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSRKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKG"
     gene            complement(3741..5363)
                     /gene="groL"
                     /locus_tag="U1099_001684"
     CDS             complement(3741..5363)
                     /gene="groL"
                     /locus_tag="U1099_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940029.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEM4867982.1"
                     /translation="MAKEIKFAADARESMVRGVDILADTVKVTLGPKGRNVVLEKAYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIEAAVATAVESLKAQASPVSNKAEIAQVAAVSSRSEKVGEYI
                     SEAMERVGTDGVITIEESRGMETELDVVEGMQFDRGYLSQYMVTDNEKMVAELENPFI
                     LITDKKISHIQDILPLLESILQTSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLDLKDATIEALGQAAKVVVDKDGTTIVEGV
                     GNAEAIANRVAVIKSQIEGTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALVNVIDSVAKLELKGDDETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIDKLKNSELGTGFNAATGEWVNMMDAGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAMPQGMDGMGMGY"
     gene            complement(5375..5656)
                     /gene="groES"
                     /locus_tag="U1099_001685"
     CDS             complement(5375..5656)
                     /gene="groES"
                     /locus_tag="U1099_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEM4867983.1"
                     /translation="MLKPLGDRIVVKIEEKEQTVGGFVLAGASQEKTKEASVLAIGQG
                     IRTLNGDLVAPAVAVGDTVLIDAHAGLEVKDGEQTVHIIREADILAIVE"
     gene            5854..6099
                     /locus_tag="U1099_001686"
     CDS             5854..6099
                     /locus_tag="U1099_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867984.1"
                     /translation="MKFFRELKTDYLESRFSVHESFAEWFLKRKLGFLGKIMFAYLLW
                     LVWLLLFSHPHYIIFFFYGVLLLSLIIMLIEWWKYRK"
BASE COUNT         1631 a         1421 c         1220 g         1866 t
ORIGIN      
        1 cgaagagtat gagtatcacg ctcagatgtg tgacatatcc gtcgtaccaa aaaacaccta
       61 cccgaaggta ggtgtagata ggcaattatg cattgccacc gtttttctta atgatttcat
      121 cttgtacaga ttttggtaca tcttcgtagt ggtcaaatac catcatgaag gtaccgcgac
      181 cttgagttgc tgaacgaaga acagttgcgt aaccgaacat ttcagcaagt ggcacataag
      241 cacgaacgat ttgtgtgtta ccacgagctt ccataccgtc aacgcgacca cgacgtgcag
      301 taacgtgacc cataacgtca cctaggttat cttctggtgc agtgatggtt acaagcatca
      361 ttggctcaag gatagttggt tgagctgatt tagcagcttc tttaagggca agagatgctg
      421 ccactttgaa ggctgtttca gatgagtcga catcgtggta tgaaccatcg taaagcttcg
      481 ctttaacgtc aacgattggg taaccagcaa gaacaccgtt cgccatagat tctacgagac
      541 ctttttcaac cgctgggatg aattcacgtg gaaccacacc accgacgata gcgttctcga
      601 actcgaaacc tttaccttct tcgtttggtg tgaattcgat ccaaacgtca ccgaattgac
      661 ctttaccacc agactggcgt ttgaagaaac cacgagcttg tgtagaagca cggaatgttt
      721 cacggtatga tacttgagga gcacctacgt ttgcttcaac cttgaattca cgacgcatac
      781 ggtcaacaag gacatccaag tgcaactcac ccataccaga gataactgtt tcaccagttt
      841 caacgtttgt ttcaacgcgg aatgttggat cttcttcagc caatttagaa agggcaatac
      901 ccatcttgtc ttggtcagct ttagacttag gctcaaccat caattggata actggttctg
      961 gaacgtggat tgactcaagg atgatttttg ctttttcatc tgtcaatgag tcaccagttg
     1021 tagtatcttt caaaccaacc gcagcggcga tgtcaccagc gtaaacagtt tcaatttctt
     1081 ggcggctgtt tgcgtgcatt tgaaggatac gtccgatacg ctcacgttta cctttagaag
     1141 tgttcaatac gtatgaaccg ctgttaagga cacctgagta aacacggaag aatgtcaaac
     1201 gacctacgaa tgggtcagtc ataatcttga aggcaagagc tgcaaatggc tcttcatctg
     1261 atgcatgacg ctcttcttca gcatctgtat ctgggttaac acctttaata gcagggatgt
     1321 caagtgggct tggaaggtag tcgataaccg catcaagcat caactgaaca cctttgtttt
     1381 tgaaggctga accacacaat actgggaaga attcaacgtt gatagttgct ttacggatag
     1441 cagctttcaa ttcttcgttt gtgatttctt caccttcaag gtatttcatc atcaattctt
     1501 catcagtttc agcaactgct tcaaccaatt tttcacggta ttcttcagct tgttcaaggt
     1561 attcagctgg aatatcttct tcaagaatat ctgtaccaag gtcgttagta tagatttcag
     1621 ctttcatctt gatcaagtcg atgataccac ggaactcatc ttctgagccg attggcaatt
     1681 ggattggatg agcatttgct tgaagacgct catgaagtgt gcttactgaa taaaggaagt
     1741 cagcaccgat tttatccatt ttgttagcga atacgatacg tggaacaccg tattcagttg
     1801 cttgacgcca tactgtttca gtttgtggct caacacctga ttgtgagtca agaacggtta
     1861 ccgcaccatc aagaacgcga agtgaacgtt gtacttcgat tgtaaagtcc acgtgtcctg
     1921 gtgtgtcgat gatgtttaca cggtggttgt tccattgagc tgttgtcgca gcagatgtga
     1981 tagtgatacc acgctcttgc tcttgctcca tccagtccat ttgcgatgca ccttcgtgag
     2041 tttcaccgat tttgtggatt ttaccagtgt agtaaagaat acgctcagtt gtcgttgttt
     2101 tacccgcgtc aacgtgcgcc atgataccga tattacgagt tttttctaat gaaaattcgc
     2161 gtgccatgag gtttgttctc ctatattttt agatttctat tctgattata ccatatttta
     2221 agaaaagcga gtgggcaaat cccacccgct taagttctta ctaatggtgt gaagtggacg
     2281 aggcaagcct caatccgatt tgtgatggtt gaacgataaa ttgctaccat aatctcaatt
     2341 gatttatgta gaactgactt aacgtcctca catcctaatt gaactcgggc tacaactcac
     2401 tgacaaaaag atgaacctca tcgattgcac agcaatctgc ttcgcttccc tattttgtct
     2461 aggagttgct tgacgccctc gtatcttaat cttagttgaa ttttagctac aaatgtctgc
     2521 caaaaagata aaatctccta gaaactgaag tttctgcgtc aattttccta ttttggctag
     2581 catttgttta acgctaaaat atcctatctt accagcggaa gtgtgcgaat gcgcggtttg
     2641 cttctgccat tttgtgagta tcttcacgtt tcttaacagc tgcacctgtg ttgtttgctg
     2701 catccatgat ttctttcgca aggcgatcga tcatagtgtg ctcaccacgg ttacgagcga
     2761 ttgttaccaa ccaacgaaga ccaagtgttg tacgacgctc tggacgaact tcaactggaa
     2821 cttggtagtt agaaccaccg acacgacgtg cacgtacttc aagtacaggc atgatgtttt
     2881 ccattgctgt ttcaaatact tcaagtgcat cgttaccagt tgcttctttg atttgatcaa
     2941 aggcaccgta aacgattgat gcagcagtac cacgtttacc gtccaacata acacggttaa
     3001 tcaaacgagt taccaatttt gagttataca atggatctgg caatacttcg cgcttaggcg
     3061 cttgattttt acgactcatt tatatttccc ctttcttagc cttttggacg tttagtaccg
     3121 tatttagaac ggccttgctt acgatcgtta acacctgctg tatcaagtgc accacgaacg
     3181 atatggtaac gtacccctgg aaggtctttt acacgtccac cacgaagaag aacaacactg
     3241 tgctcttgca agttgtgacc gatacctggg atgtaagcag taacctcgat aaggttgctc
     3301 aaacgtacac gagcaaattt acgaagggct gagttaggtt ttttaggtgt catagttccg
     3361 acacgagttg caacaccgcg tttttgtggt gatgaaacgt ttgtttgaac ttttttacgg
     3421 ctgttgtaac caacgttcaa agctggtgat ttagattttt ctactttaga cttacgtggt
     3481 ttacgtacca actggttaat tgtaggcatc tacattctcc tgtatatatt ttttattttt
     3541 ggtgataggg cattggtgac aacccctatc tgtgtgtact tttgcaacat ttgtcagcac
     3601 gtccctgtac actcttgaga gaccaaaagt aaaaagtacc gtctaatata gtaccacatc
     3661 ccaaagccat tgtcaaatag atatgtttga aattttcaag aaaaaaacac agccaatcag
     3721 ctgtgtcatt ttgctattcc ttagtaaccc atacccattc cgtccatacc ttgtggcata
     3781 gctggagcgg ctggttctgg cttgttggca acgactgctt ctgtcgtcaa aatcaagctg
     3841 gctacagaag ctgcgttttg aagggccgaa cgagtcacct taacagggtc aataatacct
     3901 gcatccatca tattcaccca ttcacctgtc gcagcattaa agcctgttcc aagctcagaa
     3961 ttcttcaatt tatcaatgat aactgatccc tcataaccgg cgttataggc aatctgacgt
     4021 actggttctt ccaaggcacg aaggacaata ttgcgtcctg tttcatcgtc gcctttcaac
     4081 tccaatttcg ctacgctatc gataacattg acaagggctg taccaccacc ggcaacgatg
     4141 ccttcttcaa ctgcggcacg tgttgcgttg agggcatctt cgatacggag tttcatttct
     4201 ttcaactcag tctcagtagc ggcaccgact ttgataacag caacaccgcc agacaattta
     4261 gccaaacgct cttgtaattt ttcacggtca aattctgaag ttgttccctc aatttgtgac
     4321 ttgataacag ccacacggtt ggcaatggct tcagcattgc caacaccttc aacaatagtt
     4381 gtaccatcct tgtcaaccac aaccttggca gcttgaccaa gtgcttcaat agtcgcatct
     4441 ttcaagtcca aaccaagatc ttctgtaatc actgtcccac ctgtcaagat cgcgatgtct
     4501 tccaacatag ctttacgacg gtcgccaaag cctggcgcct taacagcaac aacgttgaac
     4561 gtaccacgaa tcttattgag aacaagggta ggaagtgctt caccatccac atcgtcagca
     4621 ataatcaaaa gcggacgact agtctgaagg atgctttcca aaagtggcaa aatatcttgg
     4681 atgtgagaaa ttttcttatc agtaatcaag ataaatggat tttcaagctc cgcaaccatc
     4741 ttctcattat ctgttaccat gtattgtgac agataaccac ggtcgaattg catgccttct
     4801 accacatcca actcagtttc cataccgcga gattcttcga tagtgattac gccgtctgtt
     4861 cccacgcgct ccatagcttc tgaaatgtac tcaccaactt tttcagaacg tgaagatacc
     4921 gctgcaactt gagcaatttc agctttattg gatacaggac ttgcttgtgc tttcaaggat
     4981 tcaaccgcag tcgcaactgc tgcttcaatc ccgcgacgga taccaattgg gttggcacct
     5041 gcagttacgt ttttcaatcc ttcacgaaca atagcctgag tcaacacagt ggccgttgtt
     5101 gtaccgtcac cagcgatgtc attggttttt gatgccactt cagataccaa cttggcaccc
     5161 atattttcaa agtggtcttc caattcgatt tctttggcaa tggttacacc atcatttgta
     5221 atgagtggtg agccataagc tttttccaag acaacattgc gaccttttgg acccaaggtt
     5281 actttgactg tatcagccaa aatatcaaca ccacggacca tgctttcacg tgcatctgct
     5341 gcaaatttga tttcttttgc cattttccta cctcttattc tacaattgcc aaaatgtctg
     5401 cttcacggat gatgtgaacg gtctgttctc cgtctttgac ttccaatcct gcgtgagcat
     5461 caatcaaaac agtatctcca actgctaccg ctggggcaac caagtcacca ttcaaggtac
     5521 gaatcccttg accgatcgct aggacacttg cttcttttgt cttttcttga cttgcacctg
     5581 caaggacaaa gccaccaact gtttgttctt tttcttcaat ttttacgacg atacgatcgc
     5641 ccaatggttt caacatctta ttacctcctg gtctttttag cactctacac tcgcgagtgc
     5701 taactcacat ttcctattgt atcacttggt caaaaatagt caagaaaatt cttttttaat
     5761 ttacctaaaa tccgtctagg gactgaggaa acgcaacctg ttaattatgg caaaactatg
     5821 ctatactatt attaaaaagt taagggggga tttatgaaat tttttagaga gctaaaaaca
     5881 gattacctag aaagccgttt ttccgttcac gaaagttttg cagaatggtt tttaaaacgt
     5941 aaattaggct ttttggggaa aataatgttc gcctatctac tctggctagt ctggttgctt
     6001 ctcttttccc accctcatta catcatcttt ttcttctacg gagttctcct actttctcta
     6061 attatcatgc tgatagaatg gtggaaatat agaaaataag tagcaaaaaa cgccaacttt
     6121 cgtcagcgtt tttaagga
//
LOCUS       DASFSW010000116         6066 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.116, whole genome shotgun sequence.
ACCESSION   DASFSW010000116 DASFSW010000000
VERSION     DASFSW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.116"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..900
                     /gene="dcm"
                     /locus_tag="U1099_001687"
     CDS             <1..900
                     /gene="dcm"
                     /locus_tag="U1099_001687"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEM4867985.1"
                     /translation="LFFEIARAAQQIQPQFLLLENVKGLLNHDQGRTFRIILSTLNEL
                     GYDAEWQVLDSKYFGVPQHRERVYIIGHSRRRSRQFLFPIGRKSKVSPLSGPKCMGNI
                     NPSGYGLNGKVYSSSAISPTLTTNKGEGVKIAYPILTPERIEKRQKGRRVKQESGPMF
                     TLTSQDRHGVVLEKRLPPSRGVYVRSNTNKGFEAAFEGDSVNFAYPSSKVRRGRVGKQ
                     VAQTIVTGNSLGVVTNQLQIRKLTPRECFRLQGFPDWAFNRASRVTSNSQLYKQAGNA
                     VTVPVAFEIGKRLAAIEKEGESE"
     gene            897..1856
                     /locus_tag="U1099_001688"
     CDS             897..1856
                     /locus_tag="U1099_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM4867986.1"
                     /translation="MKTLTIVELFGGIGALRKALLNQEIPHQLIDYVEIDRNCVKSYN
                     VLYNENFIPQSVKGYSLPNMQIDLLMHGSPCQDFSRSGLKRGGKRGSYTRSSLLFETI
                     LILKNAVQKPKWVVWENVVGVLDKKMKPTFEAYLTELEYLGYTSCYWILDSFDFGIPQ
                     KRKRIFVVSYLGRENQFDFSKLERTQAPAIEEFLECKVAEKYTVTQPSILKYIEKACQ
                     SIFTARVHIIQEYCYTISTKQTRVPNAGFIRLGTGKYRYLTERECFRLMGFTDKDFNS
                     LRTIFPEKISKKSSILYKQAGNSIVVPVLEAIVKQIYQKELNS"
     gene            1934..2305
                     /locus_tag="U1099_001689"
     CDS             1934..2305
                     /locus_tag="U1099_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867987.1"
                     /translation="MSKLFSLPDYNSEFQLRFEDDYHKEMNVPLDYESYLHNIFDFIA
                     EQDIKNGVDVHLTDEGNLCFMAYGQSYTIRSTGVSDVVRTSVTVISKDEAGNQVDFSQ
                     HFTIPVQEKEQMNKIKSEQVL"
     gene            3096..3530
                     /locus_tag="U1099_001690"
     CDS             3096..3530
                     /locus_tag="U1099_001690"
                     /inference="COORDINATES: protein motif:HMM:NF025561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HEM4867988.1"
                     /translation="MNQALVIEKMNTTMTANEFSHCQTTEEIVEMIYQQTLHTLQTNP
                     QIQREIGTAPYAKERLEYLSKEESNVEMLLELYQTAQLIPHLMEVEKEAQTMGVILEQ
                     QLIQEWNLTEELKSKDMLKWTAQMNNLRSSIKEQVRYNLIYI"
     gene            3416..>6066
                     /locus_tag="U1099_001691"
     CDS             3416..>6066
                     /locus_tag="U1099_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEM4867989.1"
                     /translation="MEFDRGIEVQGHAEVDSSDEQPTLLDQGTGKVQSDLHLVKITKE
                     ELHDVLRKGTGTQGGRLRVAHFFASQEDIKFQAQFLQKEYGWGGASGPSFPISYQYDS
                     KGLHLTRKSDGHRESFSWRQVAGEIDALLEEETYLSDDENKLYRFNDHIFSDFESSPL
                     FEFDKQENGQIIVYMEDEMVARIDTVGQINYVLELEERFRYEIDEYARNLENSLLLEK
                     MKEHEQLDLFDFDALGEETSDETAEVEHENSEKYRIAESTTVKTEERPLNNFVLEDLP
                     GSLPPSQRLENNLAAIQIIKNIETEGRRANRQEQETLSLYVGWGGLADVFNEEKTGQW
                     ADARSKLQELLSPEEYQAAKESTLTAFYTPPLVIDSIYKVLQNLGFEKGNILEPSCGV
                     GNFIGRLPKDMNRSKVYGVELDKLSGRIAQELYQKSNVQIKGFEETRFSNNFFDVAVG
                     NVPFGDFKLTDRDYDRENFLIHDYFFAKSIDKIRSGGIIAFVTSSGTMDKQDSRVRQY
                     LANRCDLLGAIRLPNDTFKGLAGTEVTSDILVLQKRDVVREQDVDWIHLARDENGLTY
                     NKYFVEHPEMVLGQMAEVSGRFGPSLACLSDGSDLQEQLSAAIQSIQGEIPRAEQVHN
                     LDDGQDILPAMDHVKNFSYTLVDGVPYYREDSVMIRQKLSLKELNRLRAYIYVVDSLK
                     KVIQLQKENASDVLIQESQEQLNKAYDSFKAKYDFINSTLNRRNLREDSHYPLVSSIE
                     KLDKGKFVGKGDIFFKRTIAKAKVVEHCDTSHEALILSISEKGRVDFDYMTQLTDKPR
                     EELIKELKGEIYLDIPFAYEESMLHPFSEQGMEEMGLSYVPADQFLSGNIREKISIMD
                     FYIDRYQSIDNPDKEVLENINELKFQR"
BASE COUNT         2027 a          976 c         1325 g         1738 t
ORIGIN      
        1 ttgttcttcg agattgctcg agcggcccaa caaatccaac cacagttttt attgcttgaa
       61 aacgtcaaag gcttactcaa tcatgaccaa gggaggacgt tcagaattat cctttccacg
      121 cttaatgaat tggggtatga tgctgaatgg caggtacttg acagcaaata ttttggtgtc
      181 cctcaacaca gggaacgagt ctatattatt ggacattcta gaagacgaag tagacagttc
      241 ctttttccta tcggacgaaa aagtaaagtc tctcctctat caggtccaaa atgcatggga
      301 aacataaatc catcgggata tggattgaat ggaaaggtgt attcttctag tgctatttct
      361 cccactctta caacgaataa aggcgagggc gttaagattg cttatccaat tctaactcct
      421 gaaagaattg agaaaagaca gaaaggtagg agggtaaaac aagaatcagg acccatgttt
      481 acacttactt ctcaggacag gcacggtgtt gttttagaaa aacgtttacc tccgagtagg
      541 ggagtttatg tacgctcaaa taccaataag ggatttgaag ctgcatttga aggagattct
      601 gtcaatttcg cttatccgtc atctaaagtt agaagaggtc gagttggtaa gcaggttgct
      661 caaacaatag ttactggaaa ctctcttggg gttgtaacga atcaattaca gattcgtaag
      721 ctaactccaa gggagtgttt tcgtttacag ggatttcctg attgggcatt taatcgagca
      781 agtagagtaa cttcaaattc acaattgtat aagcaagcag gaaatgcagt gactgtacct
      841 gttgcatttg aaattggcaa acgtttagca gcaattgaga aggaggggga gagtgaatga
      901 agactctgac aattgttgaa ttattcggag gaataggagc cctccgaaaa gcacttctta
      961 atcaggaaat tcctcatcaa ttgattgact atgtagaaat agataggaat tgtgtaaaga
     1021 gctacaacgt actctataat gagaacttta ttccacagtc tgttaaaggg tattctctac
     1081 ccaatatgca gattgacttg ttaatgcatg gcagtccttg tcaagatttt tcacgctcag
     1141 gacttaaaag aggtgggaag agaggctcct atactagaag ttctctcttg tttgaaacaa
     1201 tcttgatatt aaaaaatgcg gttcaaaaac ctaaatgggt tgtttgggag aatgttgttg
     1261 gcgtgttaga taagaaaatg aaaccaacat ttgaagccta tctaacggaa ttggaatatt
     1321 taggctatac atcgtgttac tggatactgg atagctttga ctttgggata cctcaaaaaa
     1381 ggaaacggat ttttgtggtc agttatctag gtagggaaaa tcaatttgat ttttctaaac
     1441 tagagcgaac acaagcacca gctattgaag aatttttaga atgcaaggta gctgaaaaat
     1501 atactgtcac tcaaccgtcc attcttaagt acatagaaaa ggcatgtcag tcaattttca
     1561 cagcacgagt tcacattatt caagaatatt gttatacgat ttcgactaag caaacacgag
     1621 tacctaatgc tggatttatt aggctcggta ctggaaaata tcgttactta acagaaagag
     1681 aatgtttccg attgatggga tttactgaca aggattttaa ttcattacga acaatttttc
     1741 cagagaaaat tagtaagaaa tccagtatct tgtacaagca agcaggcaat tctattgtag
     1801 taccggtact tgaagcaatt gtaaaacaaa tttatcagaa ggagttaaac tcatgaaact
     1861 attacatcaa aaatcaattt acgaaagtaa ttatgatgaa gccttacaac atgacataga
     1921 aattgataat attatgtcaa aactgttttc acttccagat tataattctg agtttcaact
     1981 gaggtttgag gatgattatc ataaggaaat gaacgttcca cttgattatg aatcatatct
     2041 ccataacatc ttcgatttta tagcagaaca agatattaag aatggggtag acgttcattt
     2101 gactgatgaa ggaaatctat gttttatggc ttacggtcaa tcttacacga ttcgctctac
     2161 tggagtgtca gatgtggtac gaacttctgt cacagtcatc tctaaagatg aagcgggaaa
     2221 ccaggtggac tttagtcagc actttactat tccagtacag gaaaaagagc agatgaacaa
     2281 aatcaagtca gaacaggttt tatagcgaaa gaaaggagaa gtcatgcgag ttgaagaata
     2341 tgccaactat gtcggagact tatccactca ttatctttca gatgtagatg cttatcaaag
     2401 gatgctaggg cggctaggtt atgtcgttgg ttttgacttt caaaaccaat tgagtattat
     2461 tcatcagaaa ccgacagcta gcttatgctc agattttcaa tcttggaagt ccgttgacta
     2521 tattgttaga cgtggtcaaa aaggggtacc gatattatta tctaatcaat caacgactag
     2581 cgtagggtat gtctttgata ttagccagac agtcctagtg aataaggata gacgaccaat
     2641 tgaacaatgg agatatgaat caggaaaaga tttatccatc cttacagata ttattgatga
     2701 gagattgtca cttagaccgg aaaatgaaga ggcagcgatt cgttatttgg caggattagc
     2761 taccaaaggc attggtccaa aagttctagc aggaatgaat ttagatgtgg aaagtgaaaa
     2821 ctatttactt cgttttattg aacaaagttt tcaagcagta tttgctggta gacttggggt
     2881 tgagatggtc ctaaacaatg atttacttga gaatgggtta ggtcaattta gtcctagaca
     2941 gtttctgctt tttggtgatt acttatcaag aatgtcccaa aaaatttttc gtattattca
     3001 ccaaaaaaag attgagaaaa ttgcattaaa agagcagaca aaagagatta ttgagcggta
     3061 taatgatggt gtagaagata gaggaggtca accaaatgaa tcaagcatta gttatcgaga
     3121 agatgaatac gaccatgaca gccaacgagt tcagtcattg tcagacaaca gaggagatag
     3181 tagagatgat ttatcaacaa actcttcata cacttcaaac caatccacag attcagagag
     3241 agattgggac agctccctac gccaaggaac gattggaata tctgagcaag gaggagagca
     3301 atgtggaaat gttgctagaa ttgtatcaga cagcccagtt gattcctcac ttgatggaag
     3361 tagagaaaga ggctcagacg atgggggtaa tactcgaaca gcaattgata caagagtgga
     3421 atttgaccga ggaattgaag tccaaggaca tgctgaagtg gacagctcag atgaacaacc
     3481 tacgctcctc gatcaaggaa caggtaaggt acaatctgat ttacatttag taaaaataac
     3541 taaagaagaa ttgcatgatg ttcttcgtaa aggaacagga acacaaggtg ggcgactaag
     3601 agttgctcac ttttttgcta gtcaagaaga tataaaattt caagcccagt ttttacaaaa
     3661 ggaatacggc tggggtggag caagtggtcc aagttttcca atttcctatc aatatgattc
     3721 aaagggatta catttaacac gtaaatccga tggacatagg gaatcattca gttggcgaca
     3781 agttgcgggt gaaatagatg ctctattgga agaagaaacc tatctttctg atgatgaaaa
     3841 taaactttat cgttttaatg accatatttt ttctgacttt gagtcctcac cattatttga
     3901 atttgataaa caagaaaatg gacaaatcat cgtctacatg gaagatgaaa tggttgctcg
     3961 cattgatacg gttggtcaga ttaactatgt actggaatta gaagaacgat tcagatacga
     4021 aattgatgag tatgcacgaa acttagaaaa cagtctattg cttgaaaaaa tgaaggagca
     4081 tgagcaatta gacctctttg attttgatgc cttaggagag gaaacatctg atgaaactgc
     4141 agaagtagaa cacgagaatt ctgaaaaata cagaattgca gagtccacca cagttaagac
     4201 tgaagaaaga ccattgaata attttgtact tgaagatcta ccaggttcat tacctccgtc
     4261 acaacgctta gaaaataatc ttgccgcaat tcaaatcata aagaatattg agacagaagg
     4321 caggagagcc aaccgacaag aacaagaaac tctttcatta tatgttggtt ggggaggtct
     4381 tgcagatgtc ttcaatgagg agaagacagg tcaatgggct gatgctcgaa gtaaattaca
     4441 agaattatta tcaccagaag aatatcaagc agccaaggaa tcaactttga ctgcttttta
     4501 tactccacca ctcgtgattg attctattta taaagtcttg cagaatcttg gttttgagaa
     4561 aggcaatatt ctggaaccct cttgtggtgt agggaatttt atcggtcgtc taccaaaaga
     4621 tatgaatcgt tctaaggtgt atggagttga gctggataag ttgagtggta ggatagccca
     4681 ggaactttat caaaaatcaa atgtgcagat aaagggattt gaagaaacaa gattttcaaa
     4741 taatttcttt gatgtagcgg ttgggaatgt gccgtttgga gatttcaaac taactgatcg
     4801 agattatgac cgagaaaact ttcttatcca tgactacttt tttgccaagt caatcgacaa
     4861 aattcgtagt ggaggtatta ttgcatttgt aacttcttct ggaacaatgg ataaacaaga
     4921 cagtcgtgtt cgacaatatt tggccaatcg atgtgacttg ttgggtgcta ttcgattgcc
     4981 aaatgatacc tttaaggggt tagcaggtac tgaggttaca agtgatattc tagtcttaca
     5041 aaaaagggat gttgtgagag agcaggatgt ggactggata catttagcta gagatgagaa
     5101 tggccttact tacaataagt attttgtaga gcatccagag atggtcttgg ggcaaatggc
     5161 agaagtctca ggtcgatttg gtcctagttt agcctgtctt tcagatggga gtgacctaca
     5221 ggagcaactt agcgcagcta ttcaatccat tcagggagaa atcccacgtg ccgaacaagt
     5281 tcacaattta gatgatggac aggacattct tccagcaatg gatcatgtta agaatttctc
     5341 ttatactttg gttgatggag ttccatatta cagagaagac tctgtcatga ttcgtcaaaa
     5401 gttatcactt aaagaactaa atagattaag agcttacatc tacgtagtgg atagtttgaa
     5461 aaaagttatt caactccaaa aagaaaatgc cagcgatgtt ttgattcaag aaagtcaaga
     5521 gcaactgaac aaggcatatg attcttttaa agcgaaatat gattttatca actctacact
     5581 taatcgcagg aacttacggg aggatagtca ttatccctta gtatcttcta ttgagaagct
     5641 agacaaaggt aagtttgtag ggaagggcga tattttcttt aagcggacaa ttgcaaaagc
     5701 aaaggttgtc gagcattgtg atactagtca tgaggccttg attctttcaa tttctgaaaa
     5761 gggaagagtt gacttcgact atatgacaca gttgactgac aagcccaggg aggaattgat
     5821 aaaagaatta aaaggggaaa tctatttgga tattcctttt gcctatgagg agagtatgct
     5881 tcatccattt tctgagcaag ggatggaaga aatgggatta tcttatgttc ctgctgacca
     5941 attcttaagc ggtaacatcc gtgagaagat ttctataatg gatttttaca ttgataggta
     6001 tcaatcaatc gacaatccag ataaggaagt tcttgagaat attaacgaac taaaatttca
     6061 acgtga
//
LOCUS       DASFSW010000117         6063 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.117, whole genome shotgun sequence.
ACCESSION   DASFSW010000117 DASFSW010000000
VERSION     DASFSW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.117"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            153..1943
                     /gene="dnaG"
                     /locus_tag="U1099_001692"
     CDS             153..1943
                     /gene="dnaG"
                     /locus_tag="U1099_001692"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM4867990.1"
                     /translation="MLSKDKITEIKQALNIVDVIGETVALTKAGRNYVGLCPFHGEKT
                     PSFNVIEDKQFYHCFGCGKSGDVFKFVEEVRGVSFADAAAILAEKAGFQTDVTPSYHQ
                     EEKRVSPHQVLYDIHRDAAKFYHALLMTTKMGEEARAYLHQRGLTDDVIKTFQLGLAP
                     AEQNILYQKLSGQYDEESLLNSGLFNPSENNIIYDAFQARIIFPLADEYGRIVAFSGR
                     IWTEENRNNKQLAKYKNSRSTAIFNKSYELYHLDKAKAVIKKQRETYLMEGFLDVIAA
                     HRVGIDNAVASMGTALTREHVAHLAKFCKKIVLTYDGDKAGQAATMKALDELQDFQVE
                     IVSLPDNMDPDEFLQKNSEEALQQVLTKSRISDVEFLIQYLKPENPDNLQMQIEFVDK
                     IAPIIAKVSSITAQNSYIYKVADLLSDFDYNQVEQAVNAVRLNQRQERTQQVFQSQTG
                     QSYLPPVQTIARITSLIRTENHLLYRMVEHPYILNEFRLREDFYFATPELQAIYDMLK
                     KSGEITSFELSQLNDSAQQAWYRMQEERLPDEVSPHEIEELEIRRDKELLKKENQHLA
                     RKIREHSHVGNADIALDELQKLLDRRRQME"
     gene            1946..3064
                     /gene="rpoD"
                     /locus_tag="U1099_001693"
     CDS             1946..3064
                     /gene="rpoD"
                     /locus_tag="U1099_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEM4867991.1"
                     /translation="MTNKKDKKTEVTTFDVQVAEFIRNHKKQGSATDDEINDQLVIPF
                     TLDADGIDDLLQRIQDAGISIVDKEGNPSARAMQVEEEPELSDEELLGSTSAKVNDPV
                     RMYLKEIGVVPLLTNEEEQELALAVEAGDPEAKQRLAEANLRLVVSIAKRYVGRGMQF
                     LDLIQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVET
                     INKLVREQRNLLQELGQDPTPEQIAERMDMTPEKVREILKIAQEPVSLETPIGEEDDS
                     HLGDFIEDEIIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLED
                     VGKVFNVTRERIRQIEAKALRKLRHPSRSKPLRDFIED"
     gene            3449..3772
                     /locus_tag="U1099_001694"
     CDS             3449..3772
                     /locus_tag="U1099_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEM4867992.1"
                     /translation="MTYTEDQIKDIQERIFHALEDVIDPELGIDIINLGLIYEIRFIE
                     GKAEIDMTLTTMGCPLADLITDQIHDVLKDVPEVTEVDVRLVWSPAWTVQKMSRYARI
                     ALGIK"
     gene            3953..4110
                     /locus_tag="U1099_001695"
                     /pseudo
     CDS             3953..4110
                     /locus_tag="U1099_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373723.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4197..5327)
                     /locus_tag="U1099_001696"
                     /pseudo
     CDS             complement(4197..5327)
                     /locus_tag="U1099_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            5423..5746
                     /gene="tnpA"
                     /locus_tag="U1099_001697"
                     /pseudo
     CDS             5423..5746
                     /gene="tnpA"
                     /locus_tag="U1099_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1880 a         1169 c         1275 g         1739 t
ORIGIN      
        1 ttactttatc tattataccc ataaaaaata ttttacaagc aatttgtttt agaaatttta
       61 cattttccca tatttagtat acaatagagt atgttaaact aaggggagag tgttcacttt
      121 ttaaattagc tagtatgata tgaggaggga atgtgctttc aaaggataaa attactgaaa
      181 taaaacaagc tctcaatatc gttgatgtta ttggtgaaac ggttgctttg actaaggccg
      241 gacgtaatta tgtcggtctt tgcccttttc atggggagaa aaccccatct ttcaatgtga
      301 ttgaagataa gcagttctac cattgttttg gatgtggtaa gtccggagat gtattcaagt
      361 ttgtcgaaga agttcgtggt gtttcttttg cagatgcggc agctatctta gctgagaagg
      421 cagggtttca aacagatgta actccttcct atcatcaaga ggagaaaagg gttagtccgc
      481 atcaggtact gtatgatatt catcgggatg ctgcaaagtt ttatcatgcc cttttgatga
      541 caaccaaaat gggtgaggaa gctagagctt atctacacca acgtggcttg acagatgatg
      601 tcataaaaac attccagcta ggcttagctc ctgccgaaca gaatattctt taccaaaaac
      661 tatcaggtca atatgacgaa gaaagtctat tgaattcggg tttgttcaat ccaagtgaga
      721 ataatattat ttatgacgcc tttcaagcga ggattatttt ccccttggcg gatgaatatg
      781 gtcgaattgt agctttttca ggtcggattt ggaccgaaga aaatcgaaac aataaacaat
      841 tagctaagta taaaaactct cgtagtactg cgattttcaa taaaagctat gaattgtatc
      901 atttggacaa ggctaaagcg gttattaaga agcaacgtga aacctatctc atggaaggtt
      961 ttctagatgt tattgctgct catcgtgtag ggattgataa tgcagtggct tccatgggaa
     1021 cagctttgac aagagagcat gttgcacacc tagctaagtt ttgtaagaaa attgttctga
     1081 cttatgatgg ggataaggct ggacaggctg ctaccatgaa agcgttggac gaactacaag
     1141 attttcaggt tgaaatcgtg agcttgccag ataatatgga cccagatgaa tttttgcaga
     1201 aaaattcgga agaagccttg caacaggttt taaccaagtc acgaatcagc gatgtagaat
     1261 ttttgattca atacttgaaa cctgaaaatc ctgacaatct acaaatgcag attgagtttg
     1321 tagataagat tgcaccaatt attgccaaag tatcatccat tacagctcaa aattcttata
     1381 tttataaggt ggcggattta ctttctgact ttgattataa tcaggtggaa caggcagtaa
     1441 atgctgttcg tctgaaccag cgccaggaga gaacacaaca ggtatttcaa tcacagactg
     1501 gtcagtctta tttgccacca gtccaaacga ttgctcgtat aacgagtctt attcgaacag
     1561 aaaaccattt actttatcgg atggttgaac atccttatat tctcaatgag ttccgtttac
     1621 gagaagattt ttattttgcg acgccagaat tgcaagctat ttatgatatg ttaaaaaaat
     1681 ctggggaaat taccagtttt gaactatcac agttgaacga ttccgctcaa caggcttggt
     1741 atcgtatgca ggaagaacgt ttacctgatg aagtgtctcc acacgaaatc gaagaattag
     1801 aaattcggcg agacaaggaa ttgttaaaga aggagaacca gcacctagca cggaaaatcc
     1861 gtgaacattc ccatgtggga aatgcagata ttgccctcga tgagttgcaa aaactcctcg
     1921 atagaagaag acaaatggag taaatatgac aaataaaaaa gataaaaaaa cagaagtaac
     1981 tacttttgat gtacaagttg ccgaatttat ccgtaaccat aaaaaacaag gttcagctac
     2041 agacgatgaa atcaatgacc aactggttat tccatttacg cttgatgctg acggaatcga
     2101 tgatttgctc caacgtattc aggacgcagg gatttcgatt gtcgataaag aagggaaccc
     2161 ttctgctcgt gctatgcagg tagaggaaga accagaatta tctgatgaag aattgctcgg
     2221 aagcacttct gcaaaagtga acgatccagt ccgcatgtat ttgaaagaaa ttggtgttgt
     2281 acctcttttg accaacgagg aagagcaaga attggcattg gcggtcgaag caggtgaccc
     2341 agaagcgaaa caacgtttgg ctgaagccaa cttgcgtttg gttgtatcca ttgccaaacg
     2401 ctatgtcggt cgtggtatgc agttccttga cttgattcaa gaaggaaaca tgggcttgat
     2461 gaaggccgtt gataagtttg actactctaa aggtttcaaa ttttcaactt atgcaacctg
     2521 gtggattcgt caggctatca ctcgtgccat tgcggaccaa gcgcgtacaa tccgtatccc
     2581 tgttcacatg gtagaaacca ttaacaaatt ggtccgtgaa caacgcaatc tcttgcagga
     2641 attgggccaa gatccaactc ccgagcaaat cgctgagcgt atggatatga cgcctgagaa
     2701 agttcgtgaa atcctcaaga ttgcacaaga gcctgtctcg ctcgagaccc caattggtga
     2761 agaagatgat agtcatcttg gagattttat cgaagacgag atcattgaaa atccagttga
     2821 ttacacaact cgtgtcgttc ttcgtgaaca gttagatgag gtattggata ctcttactga
     2881 ccgtgaggaa aacgtattgc gtcttcgttt tggtttagat gatggtaaaa tgcgcacgct
     2941 tgaagatgtt ggcaaagtct ttaacgtaac ccgtgaacgc atccgccaaa tcgaagccaa
     3001 agccctccgt aaactccgcc acccatcccg cagcaaacca ctcagagatt ttattgagga
     3061 ttagaaaggt actgcgtacc tttctaacgc ctgcccagaa ataagaaagc aggctaaccc
     3121 agtggtgttt taacccgagc ctagaaatag gaaagcgagg ctaatctccg ggggaggtga
     3181 tcgacgtttc tcatatcatt cgaccataat gatgtgagaa actagtaagt cgtctaggca
     3241 attctattgg gaattgccgt tagttgagta gcttttcaaa gcactcaact ttggcgagtt
     3301 gaaccgctcg ctgaaaacag gaaacgagcg agattttaac cgctcccctt gaaattagaa
     3361 agggagcgta acgtccagtg gactttttga cacaaacttc gtttgtttct tttctagtat
     3421 tgaacttgtg aaaaagaaag agagaaaaat gacgtacaca gaagatcaaa ttaaagacat
     3481 tcaggagcgt atttttcatg ctcttgaaga tgtgattgac cctgagctag gtattgatat
     3541 tatcaattta gggctaattt atgaaatccg atttattgag ggcaaggctg aaattgatat
     3601 gaccttgacg accatgggtt gtccattagc tgatctaatt accgatcaaa ttcatgatgt
     3661 attgaaggat gtccctgaag taacagaagt agatgtgaga ctggtttggt caccagcttg
     3721 gaccgttcaa aaaatgagtc gttatgctcg aattgcatta ggaattaaat aaaaaaacaa
     3781 aatggtttcg aacgtttgaa gccgtttttt tattatggct tcgagccagt ttttctcgta
     3841 gagaaaaaca agaaagccac cagctatgct ggtggctgcc aaggctttga gtttccattt
     3901 ctttttcctg ttataatcta attgttcagg ttagatcaag gaggaaaaag aaatggctaa
     3961 aaccagttac agtttatcac atactaaatg gatgtgctct atcacatagt tttcacacct
     4021 aagtaccgta gaaaatccat ttactacaaa ataaggcagg acttaattga tattttccgt
     4081 catctatgtc gatgcaaagg cgtggacatc acgtcatacg catgaaaaat ttcttctctc
     4141 cttttgtccc cggaaataac tgctttaaac ttagtatcta cctttttaca agcaccttat
     4201 ccccgatttc caaataattg tggcaaataa tcctccaaat ggacagaaat atagcgttgt
     4261 ttgcggcggt aactcaattt ctccttaggg aaggtcatca tttgtaagag atgaagacaa
     4321 gccttacgaa tagcatttaa attgaaagca gctcgtttat ctaaggtttg gttcttatct
     4381 tctcggtaaa ccacatctaa caaccaatgt aggctctcaa ccgaccaatg accacgaaca
     4441 acctgagaaa aagtttgcac atcgcccttg aaactaagga taaagtaacg gacctcttca
     4501 gtgatgacac catctttatc aatgatattc ttagtcatac caatcccacg aagttttttc
     4561 catttcggat gtcgttggct cagccatttc acatcgtgag aaacccagta gtctctcacc
     4621 tcaatctgac tgcgtgcctt ttcaatggtc tgataatgac agcctttctc tgtcaacgta
     4681 gataacaatt tggcgtcact aaaatagagg tcaatatcct catggagatt gccttgattt
     4741 cctttgactg ccaagcaata atcaccctta ccgctgataa tcacatccgc aatgtctgtc
     4801 taggtgccca tggcatcaat cgtgacaaca ctcttacgaa ggtctagctg acacaagagg
     4861 cgaggaatgg ctgtgatttc attactttta tcctcaacag ctacctgccc aaggctaatc
     4921 cgattaccac catcataagc agtgacaata tgagtaggag attggtgttt ccctcggttc
     4981 ccccgaatcg tttttccgtc aacggcaatc aattttgaaa aatcagttgt ttccgaacta
     5041 gcttcgaagg acagtttcag ttcctttaag aaatctggat tgaccatact cacaacacgt
     5101 tccaaggtgt catgagaagg acaaccagca ctcaagtcaa cgtattctct caagacagat
     5161 tcgttcattt ctatgaaatc ttccatctct ttccacgttt caacccctgc taattgacaa
     5221 gcgaaaacca gaaacagaat ggtagataga ggatatcgga ttttccatgc ttgacggcta
     5281 tcatactctt ctttgtgctc ttctatggat aaaagaaaat caatcaaagt agttcacctc
     5341 tattctagtg tactactttg attgatattt ggttatgatt tccgctttct gaaaacgatt
     5401 ttttcttcat gcgtttggcg tggtggaaat cattgaggga cacatgatgc cagaccatgt
     5461 tcatatgctt gtcttgatat ccccaaaact ttcgatttcc gattttatgg gatatttgaa
     5521 aagtaaaagt gctctcatga tatttgataa acatgctaat ttaaaatata agtatggaaa
     5581 tcgaaagttt tgggctagag gctactatgt gagtatcgtt ggactgaacg aaaagacggt
     5641 agcgaaatgt attcgcgagc aggagaaaaa tgacatcgca cttgataagt taagtgttaa
     5701 agaatatgaa gattcatttt cagatagtag ttttagaaca agataaaagc ccgttgttac
     5761 gggctttagt caagtaatta tagcactaac ctgaacgaag ttcagcgaac gtctttagac
     5821 gtcgctggag agaaacggct tatagccggt gtacaagcca cccgttttca cgggtggtta
     5881 tgattttatc ctaacaattg ctgcaaccat tggctgccat agatccaaaa gaagctatag
     5941 gccacaactg agaaagggcg acaaagtaaa gtaatcataa taaatttccg atagctcatg
     6001 cttgttaatc cagtaatcat caccactatg tcagctggag aaatgggaga aagcatgcaa
     6061 aga
//
LOCUS       DASFSW010000118         6035 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.118, whole genome shotgun sequence.
ACCESSION   DASFSW010000118 DASFSW010000000
VERSION     DASFSW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.118"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(31..1029)
                     /locus_tag="U1099_001698"
     CDS             complement(31..1029)
                     /locus_tag="U1099_001698"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEM4867993.1"
                     /translation="MQFALMSGLVAFLATVLLIPRFITFYQAKRIEGQQMHEDVKQHQ
                     FKAGTPTMGGTVFLVVAILVSLLFATAFQLLTGGVLAILFILALYGVVGFLDDFLKIF
                     RKINEGLNPKQKLALQILGGIVFYFVHVRGAGGGELNVFGHMVHLGVLYFPFVLFWLV
                     GFSNAVNLTDGIDGLASISVVISLLAYSVIAFNEQKFDILLVCVTMIGGLLGFFVYNR
                     KPAKIFMGDVGSLALGGMLATISIALRQEWTLLLIGLVYVIETSSVMLQVSYFKYTKK
                     RFGEGRRIFRMTPFHHHLELGGLTGKAEKWSEWKVDFFLWSVGLIMSLITLAILYL"
     gene            complement(1031..3280)
                     /locus_tag="U1099_001699"
     CDS             complement(1031..3280)
                     /locus_tag="U1099_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM4867994.1"
                     /translation="MPRRNNKLLRYVLKKRYVPSKNRRRVGQSLLLLTVFVFFLFLIN
                     FAYIIGTDSKFGVDLSERADAVHQREVTVQAKRGTIYDRTGIPIAEDSTTYTVYAIID
                     TEYVSELKKVLYVQSSQFSKVAEIFKKHLGMETAYVMEQLNQPELKQVYFGTLGKNIS
                     YSTMSSIQEEMKAAGIEGIAFNASPGRMYPNGNFASIFVGLANPVENKDGSHSLQGVS
                     GLEYYLNDILAGQDGKVIYEKDSQGRVLPGTEKVETETVNGQDVYTTISADLQRSLET
                     NMNTFFDNAKGRYANATLVSAKTGEILATTQRPSYDSDTKEGLGQEGLLERSLLYQEA
                     FEPGSTMKVLTVASAIDNGTFDPNAYYTNNEYVIADAKINDWTLNAGYSAVTLNYAQG
                     FSLSSNVGMTLLQQQMGDEKWLNYLTKFRFGYPTRFGMGDEAPGMVPEDNIVSIAMSS
                     FGQGISANQAQMLRSFTSIANNGIMLEPKFISALYDPNTDTARVSSKEEVGNPVSEQA
                     ADDTLRYMINVGTDPVYGTLYSHDLQGPAIQVDGYDIAVKSGTAEIASEDGQGYIKGA
                     YINSVVAMIPAEDPEFIMYVTIRQPEVLNVLSWRDIVSPTLKEAMLLKDNLNLDSPAP
                     ALARVANETEYQLPDLIGKAPGESAEELRRQLVQPVILGNGAEIKKVSVEKGSKVIAN
                     QQVLLLTNKFEEMPDLYAITKENMDIFAEWTGIEVTYKGTGSKVVDQSVEVGTALNKT
                     KKITITLGD"
     gene            complement(3280..3603)
                     /gene="ftsL"
                     /locus_tag="U1099_001700"
     CDS             complement(3280..3603)
                     /gene="ftsL"
                     /locus_tag="U1099_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEM4867995.1"
                     /translation="MLQEKRREATMRVIGDKIRTFTRVEKAFYSSIVLSGLALAIGII
                     FMQTRLLQLQSGMAKVNQDISQKQIEINDAKQAVNELTRSARLMEIAEKAGLTFNNDN
                     IGVAE"
     gene            complement(3614..4564)
                     /gene="rsmH"
                     /locus_tag="U1099_001701"
     CDS             complement(3614..4564)
                     /gene="rsmH"
                     /locus_tag="U1099_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEM4867996.1"
                     /translation="MTKEFNHTTVLLHETVDMLDIKPNGIYVDATLGGAGHSEYLLSQ
                     LTDGGHLYAFDQDQTAIDHAHIRLASYIEKGQVTFIRDNFRNLKTRLAELGVTEIDGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREGHLTAYDVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQARAVKPIETTTELAELIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAIDLLALDGRISVITFHSLEDRLTKQLFKEASTIDVPKGLPFIPD
                     DLKAPLELVNRKPILPSQEELEANNRAHSAKLRVAKKVHK"
     gene            4835..5023
                     /locus_tag="U1099_001702"
     CDS             4835..5023
                     /locus_tag="U1099_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4867997.1"
                     /translation="MNRVKDYRLLLGISQLDLAKAIGVSRQTVNMIENNKYNPSLDLC
                     INLAKALQTDLNSLFWNE"
     gene            5039..5239
                     /locus_tag="U1099_001703"
     CDS             5039..5239
                     /locus_tag="U1099_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4867998.1"
                     /translation="MKKNIRQALISTLIFIPLFVLFQLWGNSGAEILHTISQAKFWLQ
                     LLIAGTIYFLGFVYILPLFDQK"
     gene            5250..>6035
                     /locus_tag="U1099_001704"
     CDS             5250..>6035
                     /locus_tag="U1099_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEM4867999.1"
                     /translation="MKKETFQDKLIKRFYGIAGPLDEFRQKEAFRLGNTCFILLFWGT
                     MAITLLALALSKRYPEVVAYGYPTALLLSTLSASMYMTSKMRHSQVDSLDVEELTTKE
                     QKKFKGASIKFALYFTCGMYIWNTGFDAWMEGLNPLDHLFDLRKFLAACLVGVFMGIY
                     IEITLRKRMKKAEKLTVSSAIAKEEPKWIKNMIKRFYGIRGPLDEYRRAEADAIGGQA
                     FIYYFYFLALGNAIAYFLAYRYPLEVAAYYPMIIAFFSIILIGI"
BASE COUNT         1747 a         1404 c         1144 g         1740 t
ORIGIN      
        1 aaaaacagca tttctgctgt ttctgacatt ttatagataa agaatagcta gggtaatcaa
       61 gctcatgatg agaccgactg accagaggaa aaagtcaact ttccactcgc tccatttttc
      121 agcttttccg gtcaaacctc caagctccaa atggtgatga aaaggagtca tacggaagat
      181 acgacgacct tctccaaagc gttttttggt gtatttaaaa tatgaaacct gcaacataac
      241 agaggacgtt tcaatgacat agaccaggcc gataaggagg agagtccatt cctgacggag
      301 ggcaattgaa atagttgcca acataccgcc gagagccaag cttcccacat cccccatgaa
      361 aatcttcgct ggtttacggt tataaacaaa gaaaccgagc aagccaccaa tcatggtcac
      421 acaaactaga agaatatcaa atttttgctc attgaaggcg atgacagagt aagctagaag
      481 gctgataaca actgagatag aagccaatcc atcaatgcca tctgtcaaat taactgcatt
      541 tgaaaaaccg accagccaga acagaacaaa tgggaaatag agaactccca aatgtaccat
      601 gtgaccaaat acattcaatt ctccgccacc tgctcctctt acgtggacaa agtagaagac
      661 aatacctcct aaaatttgta gagcaagctt ttgtttagga ttaagacctt catttatttt
      721 acggaaaatt tttaaaaagt catccaaaaa tcctactaca ccataaaggg caaggatgaa
      781 gaggatggca aggacaccac ccgtcaacag ttgaaaggcc gttgcaaaca acaagctgac
      841 aagaatcgca acaacgagaa aaactgttcc ccccattgtt ggtgtacctg ccttaaactg
      901 atgttgtttc acatcttcgt gcatctgttg cccctcaatg cgtttggctt gataaaatgt
      961 aataaatcgt gggattaaga gaacggttgc tagaaaagct accaatcccg acataagtgc
     1021 gaattgcatg ttagtctcct aaggttattg ttattttctt ggttttattc aaagcggtac
     1081 cgacctctac actttggtcg actacttttg aacccgttcc tttataggtc acttcgatac
     1141 cagtccattc agcaaatata tccatattct ctttggtgat tgcgtacaag tctggcattt
     1201 cttcgaattt atttgtcagg agtagaacct gttggttggc gattacctta cttccttttt
     1261 cgactgaaac ttttttaatt tcagcaccgt ttccaaggat aactggttga actaattgac
     1321 ggcgaagttc ctcagccgac tcgcctggtg ccttaccaat gaggtctggc aattgatact
     1381 ctgtctcatt agcgacccgt gccaatgctg gtgcaggtga atccagattc aaattgtctt
     1441 tcaacagcat cgcttctttc aatgtaggac tgacaatgtc acgccaagac aggacattta
     1501 atacttccgg ttggcgaatg gtcacataca tgataaattc tgggtcctca gcagggatca
     1561 tggctactac agagttgata taggcgcctt taatgtatcc ttgaccatct tcgctggcaa
     1621 tttccgccgt acctgatttg actgcaatat catagccatc aacctgaatt gcaggcccct
     1681 gtaagtcatg tgagtagagt gttccataaa cagggtcagt accgacatta atcatatagc
     1741 gaagtgtatc atcggcagct tgttcagaaa ctggatttcc cacttcttct ttcgaagaaa
     1801 ctctagctgt atctgtatta ggatcataaa gagctgagat aaatttaggc tctagcataa
     1861 taccattgtt cgcaatggat gtaaaactac gaagcatctg tgcttggttt gcagaaatcc
     1921 cctgaccaaa cgatgacatg gcgatagaaa caatgttatc ctctggtacc attcctggcg
     1981 cctcgtcccc cataccaaaa cgcgttgggt agccaaaacg gaacttagtg aggtaattta
     2041 gccatttttc atctcccatc tgttgctgca ataaggtcat cccaacgtta ctggataggg
     2101 agaagccttg cgcataattg agagtcacag ctgaataacc tgcattgagg gtccagtcat
     2161 tgattttcgc atctgcaata acgtactcat tattggtata atatgcattt gggtcaaagg
     2221 ttccgttatc gatagctgac gctactgtta gcaccttcat tgttgaacca ggctcaaagg
     2281 cctcttgata taaaagtgac cgctctagca ggccttcttg tcccagacct tctttggtat
     2341 ctgaatcata gcttggcctt tgggtcgtcg ccagtatttc acccgtcttg gctgaaacca
     2401 aagttgcatt cgcatatcgc cctttggcgt tatcaaagaa ggtattcata ttggtttcaa
     2461 gagaacgttg cagatcggct gaaattgtcg tatagacatc ctgcccgttt actgtctccg
     2521 tttcaacctt ttctgtccca ggtaggacac ggccttgact atctttctcg tagataacct
     2581 ttccatcttg accagctaga atatcattca gataatattc caacccactc acaccctgta
     2641 aactatgact accatccttg ttttcaaccg gattagctaa cccgacaaaa attgaagcaa
     2701 aatttccatt cggatacatc cgtcctggac tagcattaaa ggcaatccct tcaatacctg
     2761 ccgccttcat ctcttcttgg attgaggaca tcgtgctata ggagatattc ttacctaagg
     2821 tcccaaagta tacctgcttc agttccggtt gattcagctg ctccataaca taagctgtct
     2881 ccattccgag atgcttttta aagatttctg ctactttgct gaactgagaa gactgcacat
     2941 acaaaacttt tttcagttct gacacatatt ctgtgtcaat gatggcatag actgtatagg
     3001 tcgttgaatc ttctgcaata ggaattcctg tacggtcgta tatcgtcccc cgtttagctt
     3061 gaacggtaac ttcacgctgg tgtaccgcat cagctctctc tgacaagtcc accccaaatt
     3121 tactatcagt gccgataata taggcaaagt tgattaaaaa gagaaaaaag acgaaaaccg
     3181 tcaaaagaag taggctctgt cctactcttc gacgattttt tgacggaaca tatcgttttt
     3241 tcaagacata ccgcaagagt ttattatttc ttctgggcat tattcggcta ctccaatatt
     3301 gtcattatta aaagttaatc cagctttttc agcaatttcc atcaagcgag ctgaacgtgt
     3361 caattcattg acagcttgct ttgcatcgtt aatttcaatc tgtttttggc taatatcttg
     3421 attgactttg gccatgccag attgaagttg caaaagccgc gtttgcataa agataattcc
     3481 tatcgcaagg gctaatccag ataatacgat acttgaatag aaggcttttt ctacacgtgt
     3541 gaaggttcta attttatcac cgataacccg catagtggct tctctacgtt tttcttgcaa
     3601 catagactct ctcctattta tgtactttct tagctacacg cagtttagct gaatgcgcgc
     3661 gattatttgc ctctaattct tcttgactag gtaagatagg cttacgattg accaattcaa
     3721 gtggcgcctt caagtcatct ggaataaatg gcaaaccttt tggaacatca atcgtacttg
     3781 cttctttgaa gagttgtttg gtcaaacgat cttctaaaga atggaaagta atcacagaaa
     3841 tccgtccatc caaagccaat aagtcaatgg cctgctggat agattcgtct gctgccccca
     3901 actcatcgtt gacttcaatc cgaatcgctt ggaaaatctg cttggcagga tggcccttct
     3961 tcttaagttc cttggctggc ttggcagatt tgatcagctc tgccaactca gtcgttgttt
     4021 caatcggctt gaccgcacgc gcctgttcaa tcttacgagc aatctgcttg gaaaatttat
     4081 cctcaccgta tttaaagaaa atccgtacca agtcgtgata atcatagtta ttgaccacat
     4141 cataagccgt caaatgtccc tcacgattca tccgcatatc aagcggagca tcttgtttgt
     4201 aagaaaagcc acgctcccgc tcatccaact gtggactaga tactcctaaa tcataacaaa
     4261 tcccatcaat ctctgttaca cctagctccg ccaagcgagt cttgagatta cggaaattat
     4321 ccctgataaa ggtcacttgt cccttttcaa tgtaggatgc caagcgaata tgggcatgat
     4381 caatagcagt ctgatcctga tcaaaggcat aaagatgccc accatccgtc aactgactga
     4441 gcaggtactc actatgaccc gctccaccca gcgttgcatc tacataaatt ccattgggtt
     4501 tgatgtctaa catatcgact gtctcatgca agagaacagt tgtgtgatta aattccttgg
     4561 tcatatcttt tctattgtac cacaaattaa aataaaaagt tgttgcaaac tttttacaaa
     4621 aaaactgtca aatatagttg acaacttata tgaacaggta tatactaaca gtaaagaaac
     4681 atgtcacata tagttgacat caacagggag gtctcccaca tttttttaca gttatatgtc
     4741 agatataatt gacaatattt taaaaggata gtcaacctat cctctcattt ttagccaagc
     4801 atgtcagata taattgacat agaaaggagg gcacatgaac cgtgtcaaag attatcgcct
     4861 actgctaggc atttcccagc tggatctagc caaagccatc ggcgtatcca ggcagaccgt
     4921 caacatgatt gaaaacaaca aatacaaccc ctcgctagac ctctgtatca acctagccaa
     4981 agctttacag actgacctca acagcctctt ttggaatgaa tagaaaggaa gaaaaaacat
     5041 gaaaaagaat atacgtcaag ctcttatctc tacccttatt tttataccgc tctttgtcct
     5101 ctttcagctt tggggcaata gcggagcaga aatcttgcac accatcagcc aagcaaaatt
     5161 ttggctacaa ttgctgattg ctgggaccat ctacttctta ggctttgttt acatcttacc
     5221 attgttcgat caaaaataag gagctgctta tgaaaaaaga aacctttcaa gataaattaa
     5281 tcaaacgatt ttacggtatc gcagggccct tggatgaatt tcgccaaaaa gaagccttcc
     5341 gactaggcaa tacctgcttt atcctgctct tttggggcac catggctata accctgctgg
     5401 cacttgccct atccaaacgc tacccagaag tcgttgccta tggctaccca acggctctac
     5461 tcctatccac tctttccgcc agtatgtata tgacatccaa gatgcgccac agtcaagtgg
     5521 acagtttaga tgtagaagaa ctgactacca aggaacaaaa gaaattcaag ggagcaagta
     5581 tcaaattcgc cctctatttc acctgtggta tgtatatctg gaacacggga tttgatgcct
     5641 ggatggaagg actcaatcca ctagaccatt tatttgacct ccgaaaattc cttgcagcct
     5701 gtctagtcgg tgttttcatg ggaatttata tcgaaatcac tcttcgtaaa cgcatgaaaa
     5761 aggcagaaaa actaactgtc agctctgcta ttgccaaaga agaacccaag tggatcaaaa
     5821 atatgattaa acgtttttat ggtatccgcg gtcctttaga cgaataccga cgggccgagg
     5881 ctgatgctat cggcggacaa gcctttatct actacttcta cttcctagct cttggaaatg
     5941 ccatcgccta cttcctagcc taccgctacc ctctggaggt ggctgcctac tatccaatga
     6001 tcattgcctt cttcagtatt atcctaattg gtatc
//
LOCUS       DASFSW010000119         6026 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.119, whole genome shotgun sequence.
ACCESSION   DASFSW010000119 DASFSW010000000
VERSION     DASFSW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.119"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            26..1159
                     /gene="neuC"
                     /locus_tag="U1099_001705"
     CDS             26..1159
                     /gene="neuC"
                     /locus_tag="U1099_001705"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEM4868000.1"
                     /translation="MKKICFVTGSRAEYGIMRRLLSYLQDDPEMELDLVVTAMHLEEK
                     YGMTVKDIEADKRRIVKRIPLHLTDTSKQTIVKSLATLTEQLTVLFEEVQYDLVLILG
                     DRYEMLPVANAALLYNIPICHIHGGEKTMGNFDESIRHAITKMSHLHLTSTDEFRNRV
                     IQLGENPNHVLNIGAMGVENVLKQDFLTREELAMELGIDFAEDYYVVLFHPVTLEDNT
                     AEEQTQALLDALKEDGSQCLIIGSNSDTHADKIMELMHEFVKQDSDSYIFTSLPTRYY
                     HSLVKHSQGLIGNSSSGLIEVPSLQVPTLNIGNRQFGRLSGPSVVHVGTSKEAIVGGL
                     GQLRDVIDFTNPFEQPDSALQGYRAIKEFLSVQASTMKEFYDR"
     gene            1149..1799
                     /locus_tag="U1099_001706"
     CDS             1149..1799
                     /locus_tag="U1099_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEM4868001.1"
                     /translation="MIDRGESLMKKVAFLGAGTFSDGVLPWLDRSRYELIGYFEDKPI
                     SDYRGYPVFGPLQDVLTYLDDGKVDAVFVTIGDNVKRKEIFDLLAKDHYDALFNIISE
                     QANIFSPDSIKGRGVFIGFSSFVGADSYVYDNCIINTGAIVEHHTTVEAHCNITPGVT
                     INGLCRIGEGTYIGSGSTVIQCIEIAPYTTLGAGTVVLKSLTESGTYVGVPARKIK"
     gene            1808..3040
                     /locus_tag="U1099_001707"
     CDS             1808..3040
                     /locus_tag="U1099_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HEM4868002.1"
                     /translation="MEPICLIPARSGSKGLPNKNMLFLDGVPMIFHTIRAAIESGCFK
                     KENIYVSTDSEVYKEICETTGVQVLMRPADLATDFTTSFQLNEHFLQDFSDDQVFVLL
                     QVTSPLRSGKHVKEAMELYGKGQADHVVSFTKVDKSPTLFSTLDENGFAKDIAGLGGS
                     YRRQDEKTLYYPNGAIYISSKQAYLADKTYFSEKTAAYVMTKEDSIDVDDHFDFTGVI
                     GRIYFDYQRREQQNKPFYKRELKRLCEQRVHDSLVIGDSRLLALSLDGFDNISIGGMT
                     ASTALENQGLFLATPIKKVLLSLGVNDLITDHPLHMIEDTIRQLMESLVSKAEQVFVT
                     TIAYTLFRDSVSNEEIVQLNDVIVQSASELGISVIDLNEVVEKEAMLDYQYTNDGLHF
                     NQIGQERVNQLILTSLTR"
     gene            3237..4110
                     /locus_tag="U1099_001708"
                     /pseudo
     CDS             3237..4110
                     /locus_tag="U1099_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4346..4531
                     /locus_tag="U1099_001709"
     CDS             4346..4531
                     /locus_tag="U1099_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868003.1"
                     /translation="MSQPVVQLTIPQSRRFEKKSRNDILMKIRLGKTEVTFFQSINPE
                     VMETILDKVLHYDHPTQ"
     gene            4512..4799
                     /gene="tnpB"
                     /locus_tag="U1099_001710"
     CDS             4512..4799
                     /gene="tnpB"
                     /locus_tag="U1099_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEM4868004.1"
                     /translation="MTIRLSDLGQVFLVCGKTDMRQGIDSLAYLVKSQFNLDPFSGQV
                     YLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWPNNEEEVKSLTSEQVD"
     gene            4916..5122
                     /locus_tag="U1099_001711"
     CDS             4916..5122
                     /locus_tag="U1099_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM4868005.1"
                     /translation="MDELLAIIKQQTSTIEQLTNELTLLREQVDYLRQKLYGKSSEKV
                     VYQPGQLSLFGEESLLEEEADLPS"
     gene            5076..6026
                     /locus_tag="U1099_001712"
     CDS             5076..6026
                     /locus_tag="U1099_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEM4868006.1"
                     /translation="MGRKASLKKKLTYPVETEIITYKRKKTKGVRQAIFSQFTPEIVH
                     HELQGEDCTCPDCHGQLKEIGSAVQRQELVFIPAQLKRIDHVQHAYKYQACSQKNLSD
                     KIIKAPVPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWHKLGLPISRKEIANW
                     HIKSSQYYFEPIYNLLHEKLLEQPILHADETAYKVLENDGQLTFYWTFLSGKHEENGI
                     TLYHHDKRRSGLVVEEVLGDYAGYVHCDMWSAYRQLDKAQLVGCWAPVKRKFFEATPK
                     KTDKTSLGAKGLAYCDRLFALESDWADLSSEERLHKRWLP"
BASE COUNT         1780 a         1126 c         1374 g         1746 t
ORIGIN      
        1 aaaatcaaat gtaagcggag taaggatgaa aaaaatttgt tttgtgacag gctctcgtgc
       61 cgaatatggg attatgcgtc gcttattgag ctatctacag gatgatccag aaatggagct
      121 ggatcttgta gtgacagcca tgcatctaga agaaaaatat gggatgacgg tcaaagacat
      181 cgaagcggac aagcgtagga ttgtcaagcg gattccattg catttgacgg atacgtctaa
      241 gcagacaatc gtcaaatctt tagcgacctt gacagagcaa ctcacggttc tttttgaaga
      301 agtccagtat gacttggtgt tgattctggg ggatcgctat gagatgctac cagttgccaa
      361 tgctgcgttg ctttataata ttcctatttg ccatattcat ggtggtgaaa aaaccatggg
      421 aaattttgat gagtcgattc gccatgccat taccaagatg agtcaccttc atctgacatc
      481 aacggatgaa tttagaaatc gtgtcattca actaggagaa aatcccaacc atgtactgaa
      541 catcggagct atgggtgttg aaaatgtttt aaaacaagac tttttgacaa gagaagagtt
      601 ggcgatggaa cttggaattg attttgccga ggattactat gttgtactct ttcaccctgt
      661 taccttggag gataacacag ccgaagaaca aacgcaggcc ttattagatg ctctaaaaga
      721 agatggtagc cagtgtttga taattggatc caattcggat acacatgccg ataagataat
      781 ggaattgatg catgaatttg taaaacaaga ctctgattct tacatcttta cttcgcttcc
      841 aactcgttat taccattcct tggtcaagca ttcacaaggt ttaataggga attcttcgtc
      901 aggtttgatt gaagtgccct cattacaggt tccaacctta aatattggaa atcgccaatt
      961 tggacgtttg tcaggaccga gtgtggtaca tgttggaact tctaaggaag cgattgttgg
     1021 tggtttgggg caattacgtg atgtgataga ttttaccaat ccatttgaac aacctgattc
     1081 tgctttacaa ggttatcgag ctatcaagga atttttatct gtacaggcct caaccatgaa
     1141 agagttttat gatagatagg ggagaaagtt tgatgaaaaa agtagccttt ctaggagcgg
     1201 gtaccttttc agatggtgtc cttccttggt tggatagaag tcgatatgaa ctcattggat
     1261 attttgaaga taaaccgatc agtgactatc gtggctatcc tgtatttggt cccttgcaag
     1321 atgtcctaac ctatttggat gatggaaaag tagatgctgt cttcgtcact ataggtgaca
     1381 atgtcaagcg caaggaaatc tttgacttgc ttgccaaaga tcattatgat gctttgttca
     1441 acatcattag cgagcaagcc aatatttttt ccccagatag tatcaaggga cgaggggttt
     1501 tcataggttt ttcaagtttt gtaggagccg attcctatgt ctatgacaat tgtatcatca
     1561 atacgggtgc cattgtggaa catcatacca cggtggaggc ccattgtaac attactccag
     1621 gagtgaccat aaatggcttg tgccgtatcg gagaaggcac ttatattgga agtggttcaa
     1681 cagtgattca atgtatcgag attgcacctt atacaacatt gggggcaggg acagttgttt
     1741 tgaaatcgtt gacggagtca gggacctatg ttggtgtacc tgctagaaag attaaatagg
     1801 tgaattgatg gaaccaattt gtctgattcc tgctcggtca ggatcaaaag gtttaccaaa
     1861 taaaaacatg ttatttttag atggtgtacc gatgattttc cataccattc gagctgcgat
     1921 tgagtctgga tgttttaaga aagaaaatat atatgtcagt actgattcag aggtttacaa
     1981 ggaaatttgt gaaacaactg gggttcaagt cctcatgcgt ccagctgact tggcgacaga
     2041 ttttacaacc tcttttcaac tgaacgaaca ttttttacaa gatttttctg atgaccaagt
     2101 atttgttctc ctgcaagtta cgtccccatt aagatcggga aaacatgtca aggaggcgat
     2161 ggagttatat gggaaaggtc aagctgacca cgttgttagc tttaccaaag tcgataagtc
     2221 tccaacattg ttttcaactt tagacgaaaa cggattcgct aaggatattg caggattagg
     2281 tggcagttat cgtcgtcaag atgagaaaac actctactat cctaatggag cgatttatat
     2341 ttcttctaag caggcttatt tagcggataa aacttatttt tctgaaaaaa cagcggccta
     2401 tgtgatgacg aaggaagatt cgattgatgt agatgatcac tttgatttta ctggtgttat
     2461 tggtcgaatt tactttgatt accagcgtcg tgagcaacaa aacaaaccat tttataaaag
     2521 agagttaaag cgtttatgtg agcaacgagt ccatgatagt cttgtgattg gcgatagtcg
     2581 tctgttagcc ttgtcactgg atggtttcga taatatcagc atcggtggga tgacagcttc
     2641 gacagcactt gaaaaccaag gtctcttttt ggctactccg ataaagaaag ttttgctttc
     2701 tcttggtgtg aatgatttga ttactgacca tcccttgcat atgattgagg atactattcg
     2761 ccagctgatg gaaagtcttg tttccaaagc agagcaggtt tttgtgacga cgattgccta
     2821 cacgctgttt cgtgatagcg tttcaaatga agaaattgtg cagctgaatg acgttattgt
     2881 tcagtcagca agtgaactgg gtatttcagt gattgatcta aatgaagttg ttgaaaaaga
     2941 ggcgatgctt gactatcagt ataccaatga tggattgcat ttcaatcaga ttggacaaga
     3001 gcgtgtgaat cagctgattt tgacaagttt gacaagataa tttggtgata gaagctattt
     3061 cagtggctag actatgttgg tatgtgtttt agagccaagg aataacatct gtagaggatg
     3121 ctagccttga gaattgacaa ccatttagtt gtttaattat ataaggggac ctctaaaaac
     3181 tccctaaatt tcccaaaaat gagataatag aataaaaagt aatgaggaga gctgtcatgc
     3241 atttattcac agacgatgaa aaaatcttgt caaaactatc agagaaaggc aatcccttag
     3301 aacgtttgga tgccgttatg gattggaata tctttcttcc attattgtca gagttattca
     3361 gtcgtaaaga taaagtcatc agtcgtggcg gtcgtcctca cctagactat ctcatgatgt
     3421 tcaaagcgct cttgcttcaa cgtcttcata acctatctga cgatgccatg gaatatcaac
     3481 tgctggatcg tatatctttt cgtcgttttg ttggttgtca tgaagacact gttcccgatg
     3541 cgaaaactat ctggctctat cgtgagaaat taaccaagtc aggtcgtgaa aaggagttgt
     3601 tcgatttgtt ctatgcccat ctcacagatg aaggggtgat tgcccattca ggtcagattg
     3661 tggatgctac ctttgtcgaa tgccctaaac aacgcaattc acgtgaggac aatcagaaaa
     3721 tcaaaactta tcgaaaatta tgaggtcaca acagctagtg tacacgactc caatgtccta
     3781 gctcctcttt gtgatgccaa tgaagcggtt tttgatgaca gtgcttatgt tggaaaatca
     3841 gtaccagaag gttgtcgcca ccacacgatt cgtcgtgctt ttagaaataa accgttgact
     3901 gagactgata aggtcattaa tcgacatatt accaaagtcc gttgtcgcgt tgagcatggt
     3961 tttggcttca ttgaaactaa catgaaaggt aacatctgtc gagcaattgg gaaggcacga
     4021 gctaaaacca atgtgacctt aaccaacctg ctctacaata tctgtcgttt tgagcaaatc
     4081 aaacgactgg gattaccatc gtgggcttag tgcgcccaaa aataggaaaa taagcaaaaa
     4141 gaggctgggc aaaaactagt ttctcacaat aaaaaaacgg ctctttgtca actgtagtgg
     4201 gtagacgaaa agctaacacc tagagaggac gaaattcgtt ctctcatttt tgatgtttaa
     4261 agcgtaaccg cctaataaca aggtatctat ccaatcacac attcctccat tatactgtta
     4321 taaaaaacaa actggaggat tcattatgtc acagcccgtc gtccaattga ctattcccca
     4381 atctcgccgt tttgaaaaga aaagcagaaa cgatattctg atgaaaattc gtctcggaaa
     4441 aacggaagtc acattcttcc aatctatcaa ccctgaagta atggaaacaa tcttggataa
     4501 ggtgctgcat tatgaccatc cgactcagtg atttaggtca agtcttcttg gtctgtggga
     4561 aaacggatat gcgtcagggg attgactctc tcgcctatct tgtcaaaagt caatttaatc
     4621 tggatccctt ttcaggtcag gtctatctct tctgcggagg tcgaaaagac cgattcaaag
     4681 ctctttactg ggatggacag ggattttggt tattgtataa acgttttgaa aatgggaaat
     4741 tgacctggcc aaacaatgaa gaggaggtta agtccctcac ttccgaacaa gtagattagc
     4801 tcatgaaagg attttctatc attccaaaaa taaatgtttc aaaaagtcgt gatttctatt
     4861 gaaatcatgg cttttctttg tgtataatga gataaaaaga aggaggagcg agcgtatgga
     4921 tgagttgttg gcaattatta aacaacagac ttcaacgatt gaacaactta ccaatgagct
     4981 aaccctcctt cgagaacagg ttgactatct gagacagaaa ctctacggaa aatcatcaga
     5041 gaaagttgtg tatcaacccg gtcagctgag cttgtttggg gaggaaagcc tccttgaaga
     5101 agaagctgac ttacccagtt gaaacagaga tcatcactta taaacgcaag aaaactaagg
     5161 gagttcgtca ggctattttt agtcagttca ctccagagat agtgcatcac gaattgcagg
     5221 gcgaagactg cacttgtcca gactgtcatg gtcagttgaa agagattggc tcagctgttc
     5281 aacggcaaga gttggtcttc attcctgcac aattaaagcg gattgaccat gttcaacacg
     5341 catacaagta tcaggcatgt agccagaaga atctgagcga taagattatc aaggctcccg
     5401 ttcctaaggc acctttggca cacagcttgg gttcagcctc tatcatcgct cacaccattc
     5461 atcagaagtt caatttgaag gtgccaaatt accgtcagga agaggattgg cataagcttg
     5521 gcctgcccat cagtcggaag gaaatagcca actggcatat caagtctagt cagtattatt
     5581 tcgagccgat ttacaacctg ttgcacgaga aattgttgga gcagcctatt ctccatgcgg
     5641 atgagaccgc ctacaaggtc ttagaaaatg atggtcagtt gaccttctac tggaccttct
     5701 tgtctgggaa acatgaggaa aatggaatta ccctctatca tcatgacaaa cgacggagtg
     5761 gcttggttgt ggaggaggtt cttggggact atgcgggcta cgtacattgt gacatgtgga
     5821 gtgcttatcg tcaattagac aaggctcagc tggttggttg ttgggctccc gtcaaacgaa
     5881 agttctttga ggcgactcct aagaagacag acaagacttc tttaggagcc aagggcttag
     5941 cctattgcga ccgcctgttt gccttggaga gcgactgggc tgacttgtct agcgaggagc
     6001 gactgcataa acgctggctc ccttga
//
LOCUS       DASFSW010000120         5938 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.120, whole genome shotgun sequence.
ACCESSION   DASFSW010000120 DASFSW010000000
VERSION     DASFSW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.120"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            127..483
                     /locus_tag="U1099_001713"
     CDS             127..483
                     /locus_tag="U1099_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4868007.1"
                     /translation="MFSTFERIKELAKSRGVTLGGLEERLGLSRNSIYTMKNKKPSAE
                     RLQLIADYFNVSTDYLLGRTDNPRVAKTDDEIDKIDFKELAAESMSYDGKPFDEDDIE
                     FFSYIMEQHFKNKYKE"
     gene            488..877
                     /locus_tag="U1099_001714"
     CDS             488..877
                     /locus_tag="U1099_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM4868008.1"
                     /translation="MTALDLCVQQGIDILFFDGRERDKKAFFNKRANLVGIDTYVDGI
                     ERDKLLYHELGHKNHTPYQYQLHRELCELQANRNMIHHLLKDELSMLDDYNDFNYVRF
                     MERHGLKTMTDESMVIEEFRTLTGKHL"
     gene            893..2305
                     /locus_tag="U1099_001715"
     CDS             893..2305
                     /locus_tag="U1099_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEM4868009.1"
                     /translation="MKNNTNTLPFYLRGWFFLILLILSIPTYLSSLILLIGLFLIRNK
                     KYPNLSPDQQARWNEILLANEQADNILKTAKEEADNLINDAKKEAKDSIDMANTIVAG
                     VESKKNNLKEEIDKLEIAKKEAELYLSEKADALLFKETTVDFTDNITANEIKNELSLI
                     QLKEKELIKADVAINNLGIQTTKANLNKQSRQLLRAFNAESDYYVSNITAKNVDSYRN
                     KLAKSFENLNALFAVDGVKISHELLTLKLKQLDVMYKYQKQLEVERELLKAQKEEIRE
                     QQKVEKEIQQAKAKLEKEERQFQNEMSKLLKYLNSANNEVEQNIYADKIKELEDKIKE
                     LEKDKEDVLKRESNTRAGFVYIISNIGSFGQNVYKIGMTRRLEPMDRINELSSASVPF
                     PFDVHALIFSEDAPALENTLHNYFRDKEVNKVNPRKEFFKVDLQEIKELVHKEYNNTV
                     HFTDLAVAEQYYESIKLSSE"
     gene            2439..3533
                     /locus_tag="U1099_001716"
     CDS             2439..3533
                     /locus_tag="U1099_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4868010.1"
                     /translation="MAYFRKRDNGWEYRISYKAPDGSYKQKSKSGYRTKAEAVQAASQ
                     AEIELSSGIVEDKNITLAEYFEKWMLVHKKPHVGPETFGKYEYTLKLITIYFHETKLS
                     KINATSYQNIINELAKCYVKDSVKRFNSHIRAAIKVAIHQGILKKDFTEIVKIFSDVE
                     SKKEEDKYLELDEYEQVITDYRKTIKYQSHFFLYTIGKTGLRFSEAAGITEPIVDREN
                     MCLRIRRTYKVYGKKKGWGPTKNPQSERDVPFDSEWLKAYDEYMKVGYIDNPDKRLFT
                     KLTGTGENKILKKKTRQTFNVHGLRHTYVSWLIYHDVDVVTIAKLVGHKDATETLKTY
                     SHLFKAKQEESFDKVRNLMEKFGADLGRES"
     gene            complement(3571..3642)
                     /locus_tag="U1099_001717"
     tRNA            complement(3571..3642)
                     /locus_tag="U1099_001717"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3607..3609),aa:Arg,seq:cct)
     gene            complement(3840..5864)
                     /locus_tag="U1099_001718"
     CDS             complement(3840..5864)
                     /locus_tag="U1099_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEM4868011.1"
                     /translation="MKKNIRLKSSILALVAGFSVIATQAVLADELAVQIMGVNDFHGA
                     LDMTGTARLEGETVRNAGTAALLDAYMDDSQAEFEETAAETETPAESIRVQAGDMVGA
                     SPSNSGLLQDEPTVKVFNKMDVEYGTLGNHEFDEGLDEYNRIMTGEAPKKGQFNEIVD
                     NYTREAAKQEIVIANVIDKETGEIPYGWKPYAIKTIPVNDKEAKIGFIGVVTTEIPNL
                     VLKKNYEQYTFLNEAETIAKYARELAEKGVNAIVVLAHVPATSKDGVAAGEAADMIAK
                     LNEIYPEHSVDLVFAGHNHVYTNGTTGKTLIVQATSQGKAYADVRAVYDTDIADFKAV
                     PTAKIIAVAPGQKTPSPEIQAIVDEANTIVKKVTEQKIATASQATDISREVNEFKESA
                     VGNLVTSAQLAIARKSGYDVDFAMTNDGGIRADLKVQEDGTVTWGAAQAVQPFGNILQ
                     VVQMTGEQIYTALNQQYDEGEKYFLQMSGIKYIYTKADNPTEENPYKVVKAFKEDGTE
                     IVPTETYTLVINDFLFGGGDGFSIFKEAKLIGAINPDTEVFVEYLTDLEKAGQTISAT
                     IPGRKAFVEKYVEEPKAEEKEDNAGTTTDVKTPEKANDGGDSVTNQKATEQPAPSGGM
                     APISNKKTEKASGNQTLPNTGQEALGSLLISLGGLVSLGMAVSVRRKEGE"
BASE COUNT         1984 a         1145 c         1053 g         1756 t
ORIGIN      
        1 ttttttgttt cgctttatta ttcgctttac aaattatatt ctatactaaa gtttgtatac
       61 tgtcaactat ttttacaaac ttttttctag aattttttcg taatgcttag aaaggttgtt
      121 aaatcaatgt tctcaacgtt cgaaagaata aaagaattag ctaaatctag aggtgttacg
      181 ctaggtggtc tagaagaaag attggggtta agtcgaaatt ctatttatac catgaaaaac
      241 aaaaagcctt cagctgagag gctacagtta atagccgact acttcaacgt atctactgat
      301 tacttacttg gtcggactga taatccaaga gttgctaaaa ctgatgacga aattgataaa
      361 atagatttca aagagctagc agccgagtca atgtcttatg acggcaagcc gtttgacgaa
      421 gacgatatag agtttttctc atatatcatg gaacagcact ttaagaacaa atataaggaa
      481 tagtaaaatg accgcattag acctttgcgt acagcaaggt attgatattt tattctttga
      541 tggtagagaa agagataaaa aagccttctt caacaaacgt gctaatcttg ttggaataga
      601 cacgtatgta gatggtatcg aacgagataa gctactctat cacgaacttg gtcataaaaa
      661 ccatacaccc tatcaatacc aattacatag agagctgtgc gaacttcaag caaacaggaa
      721 tatgattcat catttgctga aagatgaatt gtcgatgtta gacgattata acgactttaa
      781 ctatgttcgt tttatggaac gacatggctt aaaaaccatg accgatgaaa gcatggttat
      841 tgaagaattt cgcaccctaa ctggaaaaca tttataataa ggagaacatc caatgaagaa
      901 caacaccaac actttgccct tttatttaag aggttggttt ttcttgatac tacttatatt
      961 atctatccca acttacttat cgtcgttaat cctcttaata ggattgttct tgattaggaa
     1021 taaaaaatat cccaatctct ctcccgacca acaagccaga tggaacgaaa ttcttttagc
     1081 gaatgaacaa gctgataaca tactaaaaac tgcaaaggaa gaagctgata atctaataaa
     1141 cgatgctaaa aaagaagcta aagactccat tgatatggct aatacaattg tcgcaggagt
     1201 ggaatcgaaa aagaataatc tcaaagaaga gattgataaa ctagaaatag ctaaaaaaga
     1261 ggctgagctt tatttatcag aaaaagccga tgcattactt tttaaagaaa caacggtaga
     1321 tttcacagac aatataacag ctaatgaaat caaaaatgaa ttatctttaa ttcaattaaa
     1381 agaaaaagaa ctaataaaag ctgatgttgc aataaataat cttggaatac aaacaacgaa
     1441 agctaatctt aacaaacaat ctagacaact tctccgtgca ttcaatgctg aatcggacta
     1501 ctatgtatct aacattacag caaaaaatgt agatagctat cgtaataaat tagcaaaatc
     1561 atttgaaaat ctaaatgcac tatttgcagt tgacggagta aaaatcagtc atgaacttct
     1621 cacgctaaag ttaaagcaac tagatgtcat gtataaatat caaaaacaac ttgaggtcga
     1681 gcgtgaatta ttgaaagctc aaaaagaaga aatacgcgag caacagaaag tcgaaaaaga
     1741 aatccaacaa gcgaaagcta agttggaaaa agaagaaaga cagttccaaa acgagatgtc
     1801 taaactattg aagtatctta acagcgctaa caacgaggtc gaacaaaata tatacgctga
     1861 taaaattaaa gagcttgagg acaaaattaa ggagcttgaa aaagataaag aagacgttct
     1921 caagcgtgaa agtaacacaa gagctggatt tgtttatatc atttccaata tagggtcatt
     1981 cggtcaaaat gtctacaaaa taggtatgac aagaagatta gaaccgatgg accgtatcaa
     2041 tgaattaagt agtgcttctg ttccatttcc atttgatgtt cacgctctaa tctttagcga
     2101 ggatgctcct gctctagaga atacgcttca caattatttc agagataaag aagtaaataa
     2161 agtcaatcca cgtaaagagt tctttaaagt tgacttgcaa gaaatcaaag agcttgttca
     2221 taaagaatat aacaataccg tacattttac tgatttagca gttgcggaac agtattatga
     2281 aagcataaaa ttaagttctg aataacaaat aaaaaaactc cccacgctct caaagtttgg
     2341 cgactcagag cgtgaggaga aaaaataatt ggatagtaag tggtattcga ataccttttt
     2401 tactataccc attttatcaa aaaagaaagg gtaaatcaat ggcatacttt agaaaaaggg
     2461 ataacggatg ggaataccgt atctcatata aagcccctga cggctcatat aagcaaaaat
     2521 caaagtcagg atacagaacc aaggcagagg ctgttcaggc ggcatcacag gctgaaatag
     2581 agctgtctag cggcattgtg gaagataaga acattaccct tgctgagtac tttgaaaaat
     2641 ggatgcttgt ccacaagaag cctcatgtcg gaccagaaac gtttggtaag tatgaataca
     2701 cccttaaact gattactata tactttcacg aaacaaaact ctcaaaaata aatgccactt
     2761 cctatcaaaa cattataaac gaattggcaa aatgttatgt aaaagatagt gtcaaaaggt
     2821 tcaattcgca tataagggca gcaattaaag ttgctatcca ccagggaatt ttaaaaaaag
     2881 attttaccga aattgtcaag attttctccg atgtcgaatc caagaaagag gaggataagt
     2941 acctggaact agatgaatat gaacaagtaa tcacagatta tcgaaagaca attaagtacc
     3001 agtcccactt cttcctgtac actatcggaa aaaccggact tcgtttctcg gaagcagcag
     3061 gcattacaga gcctatcgtt gaccgcgaaa atatgtgttt acgaatccgc aggacttaca
     3121 aggtttacgg aaagaagaaa ggttggggac ctactaagaa tccgcaatca gaacgagatg
     3181 tgccatttga tagtgagtgg ctgaaagcat acgacgagta catgaaagtt ggatatatag
     3241 acaatccaga taaaagattg tttaccaaat tgacagggac tggcgaaaat aaaattttaa
     3301 agaaaaagac acgtcaaaca tttaatgtac acggcttacg tcatacctac gttagctggc
     3361 taatctatca tgacgtggac gttgtgacca ttgccaagtt agtaggacac aaggatgcga
     3421 ccgaaacatt gaaaacatat tcgcacttat tcaaggcaaa acaagaagaa tcattcgaca
     3481 aagtcagaaa tttaatggaa aaatttgggg cggatttggg gcgagaaagt taaaaaccct
     3541 tgtttatcaa gggtttttgt tgtattttca tctcccctgc aggaatcgaa cctgcaacta
     3601 attcttagga ggaatttgtt atatccattt aactaaggga agtctgcttc tctattgtac
     3661 cccagaagag agcagattgc aagagcaagg ttatgtaagt tttttcaaat ttttacaaaa
     3721 aagcagaact tactctaaga tgaaatactt attattttta tgcatttctc tcatgatagt
     3781 ctctaaaaga agtaattcta gaatgtactt cgaaagtttg tcgtttttat gcttgattcc
     3841 tactcccctt ccttacgtct cactgagaca gccattccga gtgaaactaa gccacccaag
     3901 ctaataagaa gtgagcctag ggcttcttga ccggtatttg gaagtgtttg atttcctgat
     3961 gctttttcag ttttcttatt tgaaatagga gccatacctc cagatggtgc cggttgctcg
     4021 gttgctttct gatttgttac actatcgcca ccgtcatttg ctttctcagg tgttttcaca
     4081 tcagtagttg tcccagcatt atcttctttt tcttctgctt ttggttcttc tacgtacttc
     4141 tctacaaatg cttttctacc tggaattgtt gcactaatgg tttgacctgc tttttctaaa
     4201 tcagtcaagt actccacaaa tacttctgta tctggattga tagcaccaat cagtttagct
     4261 tctttgaaaa tcgagaagcc atccccacca ccaaataaga agtcgttgat gacaagtgta
     4321 taggtttctg tcggaacaat ctccgtccca tcttctttga aggctttaac aaccttataa
     4381 ggattttctt ccgttggatt gtcagccttc gtgtagatat atttaattcc agacatttga
     4441 aggaaatatt tttcaccttc atcatattgt tgatttaagg ctgtataaat ctgctcaccg
     4501 gtcatttgaa cgacttgtag gatattccca aatggttgaa cagcctgtgc tgctccccaa
     4561 gtaactgttc catcttcttg gaccttcaaa tctgcccgaa tcccgccatc gtttgtcatt
     4621 gcaaagtcaa catcataacc cgatttccta gcgatagcta attgagccga tgttactaga
     4681 ttacctacag cactttcttt aaattcattc acttcgcgtg aaatatctgt cgcttgacta
     4741 gctgtagcaa ttttttgctc tgttactttt ttaacaatgg tatttgcctc atctacaatt
     4801 gcctgaattt ccggacttgg tgttttctgc cctggtgcta ctgcaataat tttcgcagtc
     4861 ggaacagctt taaagtcggc aatatctgta tcataaacag ccctaacatc tgcgtaagcc
     4921 ttaccttgtg aggtagcttg aacaatcaag gttttgcccg ttgtaccgtt tgtatagaca
     4981 tggttatgac cagcaaatac aaggtcaact gagtgttcag gatagatttc atttagctta
     5041 gcaatcatat ctgctgcttc accagcagcc acaccatcct tgcttgtagc tggaacgtga
     5101 gccagtacaa ctatcgcatt tacacctttt tcagctaact cacgcgcata tttagcaatc
     5161 gtctctgcct cattcaaaaa agtgtactgc tcatagtttt tcttcaaaac aagattagga
     5221 atttctgtcg taactacacc aataaagcca atcttagctt ccttatcatt cacgggaata
     5281 gtcttaatag cgtagggctt ccaaccatac ggaatttcac ccgtttcttt gtcaataacg
     5341 ttagcaataa caatctcctg tttagcagct tcacgagtat aattatctac aatctcatta
     5401 aactgacctt tttttggagc ttcaccagtc atgatacggt tatactcatc aagtccctca
     5461 tcaaactcat ggttccccaa agtcccgtat tcaacatcca ttttattaaa gacttttaca
     5521 gttggttcat cttgcaaaag tccagaattc gatggacttg caccaaccat atccccagct
     5581 tgaacacgga tagactctgc aggtgtctct gtttctgctg ctgtttcttc aaattctgct
     5641 tgtgaatcat ccatgtaagc atcaagtaaa gcggcagttc ctgcattacg aactgtttcc
     5701 ccttccaatc gcgctgtccc cgtcatatca agcgcaccat ggaaatcatt aactcccata
     5761 atttggacag ctaattcatc tgccaaaaca gcttgtgttg caataacact aaaaccagct
     5821 acaagagcta gtatactgct tttcaaccga atattctttt tcataagcaa aacaatgctc
     5881 cttctatttt tttcatattg acatatctat aataatcctt ttcttacggc ttttcaat
//
LOCUS       DASFSW010000121         5909 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.121, whole genome shotgun sequence.
ACCESSION   DASFSW010000121 DASFSW010000000
VERSION     DASFSW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.121"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            42..1682
                     /locus_tag="U1099_001719"
     CDS             42..1682
                     /locus_tag="U1099_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM4868012.1"
                     /translation="MSEKVEMQQSKEQATMARGLAWLTAGNILSRLLGVAYVIPWYIW
                     LGEYRAEANALFSMGYQIYANFLLISTAGLPTAIAKQVAKYNVLGKEEVSLYLVREFF
                     KLMLVFGAVFAGVMYLSAPWLADASGSKEKLLPVMYSLVPPLFIFPAMSILRGFFQGR
                     HDMKPYAISQLAEQLVRVIWILAATFMIMKLGSGDYLEAVVQSTFAAFVGMIASVGIL
                     VYTLWKQGYLGKLLHAKKQKISLDTGQLIKETVRDAIPIIILGLTIQLLQFIDQVTFI
                     RVMERITDYSNSELLELYSYMAANPSKITMMIIGISLSLGSVAIPLITEKFVKKDLKA
                     ASHLVADNLQLLFIFTIPAIVGTVLLAGPLYTIFYGPSEPIAITLFIWNLFLILPLGL
                     YSVISVVIQAIFENRRAIYYFLIGMLVKIVLQVPMIYVFKVYGSFISTIFGLGIMLYL
                     FYRRIDKVLHIDERLVIKDIATISWISIVMGLIVWGIEFVLNLILPANGYVSSFIHLA
                     VAGGAGMLVFVILTLKTRQLDRLIGSRAQSLRRKLRLG"
     gene            1849..2583
                     /locus_tag="U1099_001720"
     CDS             1849..2583
                     /locus_tag="U1099_001720"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEM4868013.1"
                     /translation="MMKPKYERILIKLSGEALAGERGVGIDLKTVQEMAKEIQEVAES
                     GIQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLKQLGVDTRVQ
                     TAIAMQSVAEPYIRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDANAVKFNELTHREVISRGLKIMDATASTLSMDNDIDLVVFNM
                     NEPGNIKRVVFGEPIGTTVSNSSEEK"
     gene            2717..3274
                     /gene="frr"
                     /locus_tag="U1099_001721"
     CDS             2717..3274
                     /gene="frr"
                     /locus_tag="U1099_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEM4868014.1"
                     /translation="MSKEIIAKAQERMNQSHQSLAREFSHIRAGRANASLLDRISVEY
                     YGSPTPLNQLAGITVPEARVLLITPFDKSILKDIERALNASDLGLTPQSDGTVIRLVI
                     PALTEETRKNLAKDVKKVGENSKVAIRNIRRDAMDEAKKAEKAKEITEDELKTLEKDI
                     QKVTDDAIKTIDKMTADKEKELLEV"
     gene            3406..4260
                     /gene="cvfB"
                     /locus_tag="U1099_001722"
     CDS             3406..4260
                     /gene="cvfB"
                     /locus_tag="U1099_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEM4868015.1"
                     /translation="MNDLLAQYIVGLVTDENDQFYFVQKEGRTFALSKDEGEHSLGQS
                     VKGFAYTDMKQKLRLTTKEVGASRTSFGWGTVTEVRKDLGVFVDTGLPDKQVVVSLDI
                     LPEIKELWPKKGDQLYVKLDVDKKDRIWALPAFQEDFQKMAGPAYDNMQNQNLRAIVY
                     RLKMNGTFVYLPDNNMLGFIHPSERFAEPRLGQVLEARVIGYRAVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSAPEDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DSFGTELV"
     gene            4270..5190
                     /locus_tag="U1099_001723"
     CDS             4270..5190
                     /locus_tag="U1099_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEM4868016.1"
                     /translation="MLVYIAGSGAMGCRFGYQLSKTNHEVILLDNWEEHIEAIRENGL
                     KVTGDVEETVHLPIMKPAEATREADFIILLTKADQLPKMLRDIKPIIGPTTKVLSLLN
                     GLGHATTMRRYVPDESIMVGVTMWLAGLKGPGHAHLEGPGSISVQHILGDEQSVADII
                     EMLNEAGLNVTYDNHVVSAIWQKACVNGVMNPTCTILDCTIGELFGTEAGLNLVQGIF
                     KEFIAVAKSEADGIDEEAIWKYVIDASKKASNHYPSMHQDLVQHGRKTEIDYLNGAVV
                     FKGKRAGIPTPYCQMITDMIHAKESMLGLR"
     gene            5200..5655
                     /locus_tag="U1099_001724"
     CDS             5200..5655
                     /locus_tag="U1099_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEM4868017.1"
                     /translation="MLIEAETIEDYLAALPENRKEAVERLHQVIVEQLPAGFEVGILG
                     AMINYYVPLAAYPDGYHCTPGEPLPFLALASQKAHIALYHMGIYMDQELNDWFVAAYQ
                     AQVPTKLDMGKSCVRMKNPKNIPYELIGDLVSKMSMERYIELYEKNHRK"
     gene            5661..5876
                     /locus_tag="U1099_001725"
     CDS             5661..5876
                     /locus_tag="U1099_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEM4868018.1"
                     /translation="MRRSFYSWLMTQRNPKSNEPVAILADYAFDESDFPKQSDNFDEV
                     SRFLEESASFAFSMSDFDAIWEDYLGH"
BASE COUNT         1680 a         1014 c         1425 g         1790 t
ORIGIN      
        1 agtttttcct gtcttatatt tacatagaaa gtaatagaaa aatgtccgaa aaagttgaaa
       61 tgcagcagtc caaagaacag gcgacaatgg ccagaggctt ggcttggttg actgctggaa
      121 atattcttag tcgcctgctt ggtgttgcct atgttattcc ctggtatatt tggttagggg
      181 aatatcgagc tgaagccaat gccctcttta gtatggggta tcaaatctat gccaactttt
      241 tattgatttc aacagctggt cttccgacag ctattgctaa acaagtagct aaatacaatg
      301 tcttgggtaa agaagaggtg tcgctttact tggtcagaga attcttcaag ctcatgttgg
      361 tctttggagc tgtctttgca ggggtcatgt atctaagtgc tccgtggttg gcagacgcat
      421 cggggtcgaa ggaaaagtta cttccggtca tgtatagtct agtcccgcct ctctttatct
      481 tcccagccat gagtatcttg cgtggttttt tccaaggtcg gcacgatatg aagccctatg
      541 ctatcagcca gcttgcagag cagctggtac gggttatctg gatactggca gcaacattta
      601 tgattatgaa gttgggtagc ggtgactatc ttgaagcggt tgtccaatct acctttgcag
      661 cttttgtcgg gatgattgct agtgttggga ttttagttta tactttatgg aaacaaggtt
      721 accttggtaa gttacttcat gctaagaaac aaaagatttc tttggataca ggtcaactta
      781 ttaaggaaac tgttcgagat gcgattccaa ttatcatttt aggtctaacg attcaattat
      841 tgcagtttat tgaccaagtc acctttattc gtgttatgga aagaattacg gattacagta
      901 attcggaact tttggaactt tattcatata tggctgccaa tccaagtaag attacgatga
      961 tgattatcgg gatttcactg agtttgggaa gtgttgctat tccattgatt acagagaaat
     1021 ttgtcaagaa agatttgaag gcggcgtctc atttagtggc ggataatcta caattactct
     1081 ttatattcac tataccggca attgttggaa cagtcctgtt agctgggcca ctctatacga
     1141 ttttttacgg accgtcagag cctatcgcca ttactctatt catttggaac ttatttttaa
     1201 ttttacctct gggactatat tctgtcatta gtgtggtcat tcaagcgata tttgaaaata
     1261 gaagagccat ttattatttc ttgattggga tgctagtgaa aatcgtttta caagtaccaa
     1321 tgatctatgt ctttaaggta tatggtagct ttatctcaac gatatttggg ttgggaatta
     1381 tgctgtacct cttctataga cgaattgaca aggttctgca tattgatgaa agactagtga
     1441 taaaagatat tgcgaccatt agttggattt ctatcgtaat gggactcatt gtatggggga
     1501 ttgaatttgt cttgaatctt atccttccag caaatggcta tgtatctagc tttatccact
     1561 tggctgttgc aggtggagca ggtatgcttg tctttgtcat cttgacattg aagactcgtc
     1621 agctagatcg tttaattgga agtcgggctc agagtttacg gaggaaattg cgcctaggct
     1681 agtttgtttt aaaaatctct attttctact attgaaaagc tgggaaaaag aggtgtttta
     1741 gaatgtgaga aggaaagttc gttgaggatt ttcctttttt cttggagatt tcagtataaa
     1801 aaaaacggtt tttgtggtag aatagtaggg ataaaaaaga ggagagagat gatgaaacct
     1861 aaatacgaac gtattctaat caaactatct ggtgaagcct tggctggtga acgtggtgtc
     1921 ggtattgacc ttaaaactgt tcaggaaatg gcaaaggaaa ttcaggaagt cgcagaatca
     1981 ggtattcaga ttgcccttgt tattggtggt ggtaaccttt ggcgtggaga acctgctgct
     2041 gaagcgggta tggatcgagt tcaagcagac tatacaggca tgttggggac tgttatgaat
     2101 gctcttgtaa tggctgattc ccttaaacag cttggtgtgg atacacgtgt gcagacagcg
     2161 attgcgatgc agtcagttgc agagccttat attcgtggtc gtgctcttcg tcatcttgaa
     2221 aaaggtcgca ttgttatttt tggagctggt attggttctc cgtacttctc aacggatacg
     2281 acagcggctc ttcgtgctgc agaaattgaa gcggatgcga ttttgatggc taaaaatggt
     2341 gtggatggcg tctacaatgc tgatccgaag aaagatgcta atgcagttaa attcaatgaa
     2401 ttgacccacc gtgaagtgat tagtcgtggt ttgaaaatca tggatgcgac agcatcaact
     2461 ctctcaatgg acaatgatat tgatttggtc gtgttcaata tgaatgaacc aggaaatatt
     2521 aaacgagttg tctttggtga gccaatcggt actactgttt caaattcttc agaagaaaaa
     2581 taaatcatat cgtcatttag tttttctttt attacttcgt taattcgcct tgcctaactt
     2641 cggttatacc tgtggctcgt tgcttagtac taaaagtaaa ctaaaagact ataaaaaata
     2701 caaagaagga agtcttatgt ctaaagaaat tattgctaaa gcgcaagagc gcatgaacca
     2761 atctcatcag agtttggcac gtgaattcag ccacattcgt gctggccgtg ccaatgctag
     2821 tttgttggac cgtatttcag ttgagtacta cggttctcca acgccattga accagttggc
     2881 tggtattaca gtgccagaag cgcgtgttct tttgattact ccgtttgata agtcaatctt
     2941 aaaggatatt gagcgtgctt tgaacgcttc agatcttggt ttgacaccgc aatctgacgg
     3001 tactgttatc cgtttggtta ttcctgccct tacagaggaa actcgtaaga acttggcgaa
     3061 ggatgtgaaa aaagtcggtg aaaattcaaa agttgccatc cgtaatatcc gtcgtgatgc
     3121 catggatgaa gctaaaaaag ctgaaaaagc aaaagaaatc acagaagacg agttgaagac
     3181 gcttgaaaaa gatattcaaa aagtaacaga tgatgctatc aagacaattg ataaaatgac
     3241 tgccgacaaa gaaaaagaat tgttggaagt ttaatcaaca cgtttttaga ctacaaaaat
     3301 agatcatcac tgggctgtct aacttccagt ggtgaaccaa taagaaatct aatgggggga
     3361 gttagacaag acttccccca ctttttaatc agaaagaaga aaatcatgaa tgatttatta
     3421 gcacagtata tcgttggttt agtgactgac gaaaacgatc agttttactt tgtccaaaaa
     3481 gagggtagaa cctttgctct ttctaaggat gaaggagaac atagtctagg tcaatctgtt
     3541 aagggatttg cctataccga tatgaaacaa aaactccgtt tgacaactaa ggaagttggg
     3601 gcgagtcgta ccagctttgg ttggggaact gtcacagagg tacgtaagga tttgggtgtc
     3661 tttgtggata caggtctgcc agacaagcaa gtggttgttt ccttggacat tttgcctgaa
     3721 atcaaggaac tctggccgaa aaaaggggat cagttgtacg tcaaattaga cgtcgataag
     3781 aaggatcgca tttgggctct gccagctttt caagaagatt ttcagaaaat ggctggacct
     3841 gcttatgaca atatgcaaaa ccaaaatctg cgagcgattg tctatcgttt gaaaatgaat
     3901 ggtacttttg tttatttgcc agacaacaac atgcttggtt ttatccaccc aagtgaacga
     3961 tttgcggaac cacgtctggg tcaggttttg gaggcgcgtg tcatcggcta ccgtgcagtt
     4021 gatcggacct tgaacttgtc tctcaagcca cgttcatttg agatgctgga aaatgatgcc
     4081 cagatgatat tgacttatct ggaaagcaat ggaggtttca tgaccttgaa cgataagtct
     4141 gctccggaag acatcaaggc cacctttggt atttccaaag ggcaattcaa gaaagctctt
     4201 ggtggtttga tgaaggctgg aaaaattaaa caggattctt ttggaacaga gttggtctag
     4261 ggggcgtcta tgctagtata tattgctggt agtggggcaa tgggttgtcg ttttggttat
     4321 caattgtcta agacaaatca tgaagtgatt ttgctggata attgggaaga acatatcgag
     4381 gcaattcgag aaaacggctt gaaagtgact ggagatgtag aggaaacagt acacttaccc
     4441 atcatgaaac cggccgaagc aacgagagaa gcagatttta tcattctatt gacaaaggct
     4501 gatcagttgc caaagatgtt gcgggatatt aaaccgatta tcggtccaac taccaaggtt
     4561 ttgagtctct tgaatggttt ggggcatgcg acgaccatgc gtcggtacgt gccagatgaa
     4621 agtattatgg ttggggtgac catgtggttg gcgggactaa aggggcctgg acatgctcac
     4681 ttagaaggtc cgggttctat ctccgttcag catattcttg gtgatgagca atcagttgcg
     4741 gatattattg aaatgctgaa tgaagctggg ctaaatgtga cctatgacaa tcatgttgtc
     4801 agtgccattt ggcagaaagc ctgtgtcaat ggtgtgatga atccaacttg taccatttta
     4861 gactgtacaa ttggtgaatt atttggtacg gaggctggac tgaatctagt acaggggatt
     4921 ttcaaagaat ttattgctgt tgctaagtct gaggcagatg gaattgacga agaagctatt
     4981 tggaaatatg ttattgatgc ttcgaagaag gcttccaatc actatccatc tatgcaccag
     5041 gatttggtcc aacatggtcg caagacagaa attgattatc tgaatggagc agttgttttt
     5101 aagggcaaac gtgcaggtat ccctacgcca tactgtcaga tgataacgga catgattcat
     5161 gccaaagaga gtatgctggg gttgagatag gagaaagtca tgctgattga agcggaaacg
     5221 attgaagact atctggctgc cttgcctgaa aatcggaagg aagcagttga acgactccat
     5281 caagtgattg tggaacagtt accagctggt tttgaagtag gaatcttggg agctatgatt
     5341 aactattatg tgccgcttgc tgcctaccct gatggttatc attgcacacc gggagagccc
     5401 ctgcctttcc ttgccttggc atcacaaaag gcacacatcg ccctctacca catgggaatc
     5461 tatatggacc aagaattgaa tgattggttc gttgctgctt atcaagcaca ggtgcctacc
     5521 aaactggata tgggcaagtc ctgtgttcgt atgaaaaatc caaagaatat tccttatgaa
     5581 ttgattgggg atttagttag taagatgtct atggagcgct atatagagct ctacgaaaaa
     5641 aatcatagaa agtagatgta gtgagaagaa gtttttattc ttggttgatg acccagcgca
     5701 acccgaaaag taatgaacca gtagctatct tggcagatta tgcctttgat gaatctgatt
     5761 ttcctaagca atcagataat ttcgatgaag tcagtcgttt tttggaggaa tcggccagct
     5821 ttgccttttc catgtctgat tttgatgcga tttgggaaga ttatttggga cattgatgga
     5881 taaaatagta caaaatagcc ttgtttggg
//
LOCUS       DASFSW010000122         5811 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.122, whole genome shotgun sequence.
ACCESSION   DASFSW010000122 DASFSW010000000
VERSION     DASFSW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.122"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(37..312)
                     /locus_tag="U1099_001726"
     CDS             complement(37..312)
                     /locus_tag="U1099_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868019.1"
                     /translation="MITDQDYRWIADDVYKVDPLKTDDTLKQGGRAGQGQYVIIEPPV
                     DAGNGMQAMAVAPIKGYDSDNKPIPDTSQVVIASFPYPDSSVNTEYL"
     gene            complement(312..722)
                     /locus_tag="U1099_001727"
     CDS             complement(312..722)
                     /locus_tag="U1099_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868020.1"
                     /translation="MSPKRTRLILVMSAICIAIGGMIMFSFHRMSEEEKLQAQIRKEQ
                     ERMVLYAVNHYEGIEKIEFTSFRENRMTGAWYAGAILNDDYKVTITAMGFDGDLSMND
                     GPSDKTGLYLRIKDKPTTISNPNHIEVIYFKGDN"
     gene            complement(800..1033)
                     /locus_tag="U1099_001728"
     CDS             complement(800..1033)
                     /locus_tag="U1099_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868021.1"
                     /translation="MITVQDYRWIAEDVYKVDPLKTDDTFKDGDRVAQDKFVILSQPQ
                     DMINGMQAMAVAPIKGYDAENKPIPDTSQVVIA"
     gene            complement(1030..1443)
                     /locus_tag="U1099_001729"
     CDS             complement(1030..1443)
                     /locus_tag="U1099_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868022.1"
                     /translation="MFVCKKRLVLFTGLLFLSIGGMMMFNFHRMSEEEKLQAQIRKEQ
                     ERMVLYAVNHYEGIEKIEFTSFEENKMTGSWSAGAILNDDYKVTITAMGFDGDLSMTN
                     GPSDKTGLYLRIKDKPTTISIPNQIEVIYFEGDNQ"
     gene            complement(1566..2507)
                     /gene="msrB"
                     /locus_tag="U1099_001730"
     CDS             complement(1566..2507)
                     /gene="msrB"
                     /locus_tag="U1099_001730"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEM4868023.1"
                     /translation="MKEIYLAGGCFWGMEGYFSQIDGILDTSVGYANGRVETTNYQLL
                     KQTDHAETLYLAYDETRINLREILLYYFRVIDPFSVNQQGPDKGRQYRTGIYYTDKAD
                     LPTIEQVMMEQSQLFGGRPLAVEVEPLAHYIPAEDYHQDYLKKNPQGYCHIDLGQAKI
                     PLIDVADYQKPDQQVLKDSLTDLQYQVTQEAATERPFENEFWNSDQAGIYVDITTGEP
                     LFLSTDKFDLGCGWPSFTKPISKEVATYFQDKSHGMNRIEVRSRAGHAHLGHVFDDGP
                     RDKGGLRYCINSAALRFIPREEMEAAGYGLFLDLLSE"
     gene            complement(2547..4076)
                     /locus_tag="U1099_001731"
     CDS             complement(2547..4076)
                     /locus_tag="U1099_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEM4868024.1"
                     /translation="MSTISYKPLNLYRQFKTAAQEFPNVAIYFDKPYASFPELGLENT
                     YQTVFEAIQKRAAQFSAAGIGYGDKVILYKSPAFDTYLLAVAVTALGAVPVMISYHLP
                     SSNLDVFAERLEKSFIVYDQETEERVAGMTRTDLVTKVFLPQVLVQEAVAFEENLLPE
                     DVIQYMTHTSGTTGVPKLICHTAQTMGWRVAWQQTIFDKMTERGLLAFHISPVHSRYN
                     IGVSSAIGLGFPLYPLSSARKDDVERALALHRPSALETHPNNFVQWARLAKEKPEVFS
                     SIRYYHSTFDAINIGTLRAFLEASKEQHPVFMQVYGQSECGPMILRYHRLENLGTVSG
                     RDMGIGLEGYTEARITDAQGNPLPAGENGHIQFLSKGRAVTYYKEDARFQDNVYGAWW
                     DSGDYGCMTPEGTLLLKDRQVDLIEHIDSNLALEDLLLDKLDFLSEVVIIRDVNGAPQ
                     PIIALVEDAEMNWEAWWAMVADLPLLKEPILMAYDDIPRTATMKVQRLKMEAEMKEKN
                     L"
     gene            complement(4209..4523)
                     /gene="trxA"
                     /locus_tag="U1099_001732"
     CDS             complement(4209..4523)
                     /gene="trxA"
                     /locus_tag="U1099_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEM4868025.1"
                     /translation="MVQVITDANFEVETQEGVVLVDFWAPWCGPCRMQAPILEQLADE
                     VDEDELRIYKMDVDENPNTARQFGIMSIPTLLFKKDGQVVKQVAGVHTKNQIKAILAE
                     IG"
     gene            complement(4763..5083)
                     /locus_tag="U1099_001733"
     CDS             complement(4763..5083)
                     /locus_tag="U1099_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868026.1"
                     /translation="MHQLYQMLQDLYTKADQIDYATINAIEKEIERWLIRSIYAPLKL
                     REKNAILKQHKKKIQTIGNDGENSVKLIANSFDTSIKGEFSKKVQQVVKEKAKEYTKL
                     EEVE"
     gene            complement(5096..5482)
                     /locus_tag="U1099_001734"
     CDS             complement(5096..5482)
                     /locus_tag="U1099_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868027.1"
                     /translation="MDQYDKLKAELMKKEWEWEDIENQQRKAQKELQEHYENVEETTR
                     ILTRMLEEKYQEVLLELRQVGDETGDLHHLLNNGMSEWHTAIDQERYSSIHRLDQKQE
                     DLDTYYKNQYRKMQDQIDEIYTKYRE"
     gene            complement(5461..>5811)
                     /locus_tag="U1099_001735"
     CDS             complement(5461..>5811)
                     /locus_tag="U1099_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEM4868028.1"
                     /translation="AQGLVKVAETTHQTLEKETTSTLTEVNEVYSSLGNVPFGFILSP
                     DEVRQAYSSAGVDYHSLVGDSTNQVEKFITRSNQLKQDLVDLESQIQAGIEQKVTEDQ
                     TLAQRIQEWTSTIN"
BASE COUNT         1532 a         1307 c         1138 g         1834 t
ORIGIN      
        1 tttttggaca tataaaacct ccttaagttg attatactat aaatactcgg tatttacact
       61 agaatcagga taaggaaaac tggcaataac gacttgggaa gtatctggga tcggtttgtt
      121 gtcagagtca tagcccttaa tcggagcaac cgccatggcc tgcataccgt tgcccgcatc
      181 aacgggtggc tcgatgatga catactgccc ttgtcctgct ctaccgcctt gttttaaagt
      241 gtcatctgtt ttcaacggat ctactttata tacatcatct gcaatccagc gataatcctg
      301 atctgttatc attagttatc ccccttaaaa tagattacct caatgtgatt tggatttgaa
      361 atggttgtcg gcttgtcttt aattcgtaaa tacaaacctg ttttgtctga aggaccatca
      421 ttcatcgaca aatctccatc aaatcccatt gctgttatcg taactttata atcatcattt
      481 aatatagcac ctgcatacca agcacctgtc atcctatttt ctctaaaact ggtaaactcg
      541 atcttctcaa tcccttcata gtgattgacg gcatacaaga ccatacgctc ctgttcctta
      601 cggatttgag cctgcaactt ctcttcctca ctcatgcgat gaaaactaaa cattatcatt
      661 cctcctattg cgatacaaat ggcactcata accagtatga ggcgggtacg ttttggggac
      721 atataaaacc tccttatgtt gattatacta taaatactcg gtatttacac tagaatcaag
      781 ataagaaaac tatcttatac taggcaataa cgacttggga agtgtctgga atcggtttgt
      841 tttcagcatc gtagccctta atcggagcaa ccgccatagc ctgcatacca ttgatcatat
      901 cttggggctg ggagaggatg acgaatttat cttgtgcgac tcggtcccca tccttaaaag
      961 tgtcatctgt tttcaacggg tctactttat atacatcctc agcaatccag cgataatcct
     1021 gaactgttat cattggttat ccccctcaaa atagattacc tcaatttgat ttggaattga
     1081 aatggttgtc ggcttgtcct taattcgtaa atacaaacct gttttgtctg aaggaccatt
     1141 tgtcatcgac aaatctccgt caaatcccat tgctgttatc gtaactttat aatcgtcatt
     1201 taatatagca cctgcagacc aagatcctgt catcttattt tcttcaaagc tggtaaactc
     1261 gatcttctca atcccttcat agtgattgac ggcatacaag accatccgct cttgttcctt
     1321 tcggatttga gcctgcaact tttcttcctc actcatgcgg tgaaaattaa acatcatcat
     1381 tcctcctatt gacaggaaca acaaaccggt gaataaaact agtcgttttt tgcatacaaa
     1441 cataaaaatc ctcctaatat aattatatta ttcttgaaca taacttttca aataaaaagg
     1501 ataataaaga gcttaagttc aataccttaa gctctttaat ctatttactt tgaagtgttt
     1561 ccctgtcatt ctgacagcaa atccaaaaac aagccatatc ctgctgcctc catttcctct
     1621 ctcggtatga agcgaagggc tgcggagttg atacagtaac ggagaccgcc tttgtcacgt
     1681 ggtccgtcat caaagacatg acctaaatga gcatgaccag cccgactgcg gacttcaata
     1741 cggttcatgc cgtgggactt atcttggaaa taagtcgcca cttccttgct gattggtttg
     1801 gtaaaggagg gccagccgca gcctaaatcg aatttgtctg tcgagagaaa gaggggctca
     1861 ccagtcgtaa tatcgacgta gatgccagct tggtcgctat tccaaaactc attttcgaag
     1921 ggtctttccg tcgcagcttc ttgagtgact tgatactgga ggtcggtcaa gctgtctttc
     1981 aagacttgct ggtctggctt ttggtagtct gcaacatcaa tcagagggat ttttgcctgc
     2041 ccaagatcga tatggcagta cccttgggga tttttcttga gataatcctg atggtaatct
     2101 tcagcgggga tgtaatgtgc gagtggctcc acttcaacgg ctagggggcg tccgccaaag
     2161 agctgggact gctccatcat gacctgctcg atggtcggca aatcagcctt atctgtgtaa
     2221 tagataccag tccgatactg gcgacctttg tcaggtccct gctgattgac agagaaggga
     2281 tcaatgacgc ggaaataata gaggagaatt tcccgcaaat tgatacgagt ctcatcatag
     2341 gctagataga gtgtctctgc gtggtctgtt tgtttgagga gttgataatt ggtcgtctcc
     2401 acccgtccat tggcatagcc aacgctggtg tcaagaatac catcgatctg ggaaaagtag
     2461 ccttccatcc cccagaaaca accgcctgct aggtagattt ctttcatgtg aactccttct
     2521 tatctgcaag cgtggtttcg tcacccttac agattttttt ctttcatttc tgcttccatt
     2581 ttgaggcgtt gaaccttcat ggtcgctgtt cgtggaatat cgtcataagc catcaagata
     2641 ggctctttca atagtggtag gtctgcaacc atggcccacc aagcttccca attcatctca
     2701 gcatcttcta ccagagcgat aatcggctgc ggtgcaccat tgacatcgcg gataatcacg
     2761 acttctgata aaaagtccaa tttatccagc aataagtctt ccaaggcaag gttgctgtcg
     2821 atgtgctcaa tgaggtcaac ctgacggtct tttagaagaa gcgtcccctc tggagtcatg
     2881 cagccgtagt cgccactatc ccaccaagca ccgtacacat tatcttggaa gcgggcatct
     2941 tctttgtagt aagtcacagc acggcctttt gacaggaact ggatatgtcc gttttctcca
     3001 gctgggagag gattcccttg ggcatctgtg atgcgtgctt ctgtgtaacc ttccagacca
     3061 atccccatat ctcgtccgct aactgttcct aggttttcca agcgatggta acgtaaaatc
     3121 attggaccac attcactttg accataaacc tgcataaaga ctggatgctg ctctttagaa
     3181 gcctccaaga aggcgcgaag cgtaccaata ttgatagcat caaaggtcga atggtaataa
     3241 cgaatgctac taaagacctc tggtttttcc ttggccaatc ttgcccattg gacaaagtta
     3301 ttcggatggg tttccagagc acttggacgg tgaagagcaa gcgcccgttc aacatcatct
     3361 tttcttgcag aggaaagcgg gtagaggggg aatcctagcc caatcgccga cgacacgccg
     3421 atattatagc gtgaatgtac aggggagata tggaaggcca acaagcctct ctccgtcatc
     3481 ttgtcaaaaa tagtttgttg ccaagctact cgccagccca tggtctgtgc tgtgtggcag
     3541 attagttttg gaacacctgt cgtccctgaa gtatgagtca tgtattgaat aacatcttct
     3601 ggcaggagat tttcttcaaa agcaactgct tcttgcacta ggacttgggg taaaaatacc
     3661 tttgttacca ggtcagttcg agtcattcct gctacacgtt cttccgtttc ttggtcgtag
     3721 acaatgaaag acttttccaa tcgctccgca aacacatcta aattcgaaga tggcaaatgg
     3781 taggaaatca taacaggaac agcacccaat gccgttaccg ctactgctaa caaataggtg
     3841 tcgaaagctg gactcttata tagaattacc ttgtccccat agccaatacc tgctgctgaa
     3901 aattgggcag ctcttttttg aatagcttca aaaactgttt gataggtatt ttccaatcct
     3961 aattcaggga aagatgcgta gggtttgtca aaataaatgg caacatttgg aaactcttga
     4021 gctgctgttt taaactgacg atagagattt aatggtttgt aactaatagt tgacatatct
     4081 aacctttcta aaatgacaat gataaggtta ttataacatc ttgtgccaac cttcacaatc
     4141 tgatatcttt ttatgactca cagttgtgaa aaaacaagta gtcaatcacc acttgctttc
     4201 attttcaatt aaccaatctc tgccaagata gctttgattt gatttttcgt gtggacacca
     4261 gcaacttgtt tcacaacctg tccatctttt ttgaacaaaa gagttgggat agacatgata
     4321 ccaaactgac gagctgtgtt tggattctca tcaacatcca tcttgtaaat acgcaattca
     4381 tcttcgtcca cttcgtctgc taattgttcc aagatcggtg cttgcatacg gcatggacca
     4441 caccatggtg cccaaaaatc tacaaggact acgccttctt gtgtttcaac ttcaaagttt
     4501 gcatctgtaa taacttgaac cataattttc tccgtttctg tttttctact atcattctac
     4561 accaaaaaaa aacagaaagc gaatttttgc tacgggatga agcatgagat aaacgtatct
     4621 ataagaattt cagttgtaaa aaaatgggta tagctaaact agaccgcagg aggacgaggg
     4681 ggaactggga gactgttctt cctataaaat ccagttttag caatgggaaa agatttacga
     4741 aaggatgcta cgggatgaga tattactcta cctcttccaa ttttgtatat tcttttgcct
     4801 tttctttcac aacttgttgt accttttttg aaaactcgcc ttttatactt gtatcaaaac
     4861 tgtttgcaat caattttacg gaattttctc catcatttcc aattgtttga attttcttct
     4921 tgtgttgttt taaaattgca tttttttctc gcagtttgag tggagcatag atagagcgaa
     4981 tcagccaacg ttcaatttct ttctctatag cattaatcgt cgcatagtct atctggtcag
     5041 ctttggtata taaatcttgc aacatttgat aaagttgatg cattggctct cccttctatt
     5101 ctcggtattt tgtataaatt tcgtcaatct gatcttgcat ttttcggtac tggtttttat
     5161 agtaggtatc caagtcctct tgtttctgat ccaatcggtg aatggagctg tatcgctctt
     5221 gatcgattgc ggtatgccat tcggacatgc cattgttgag taaatggtgc aagtctccag
     5281 tctcgtctcc gacttgtcgc aattctaaca gaacttcttg atacttttct tccaacattc
     5341 gagtcaatat tcgagtggtt tcttcaacat tttcatagtg ttcttgtagt tctttttgag
     5401 cttttcgttg ttgattctca atatcctccc attcccactc tttcttcatt aactctgcct
     5461 ttaatttatc gtactggtcc attcttgtat cctctgtgct aatgtctggt cttccgttac
     5521 tttctgctcg atccctgctt ggatttgact ttctaagtcc actaaatcct gcttgagttg
     5581 attggagcga gtgataaact tctcgacttg attggtgcta tctcctacca gactatgata
     5641 gtcaacacct gcactgctgt aggcctgtct cacttcatca ggactcaaaa tgaagccaaa
     5701 agggacattt cctaagctac tgtagacttc atttacttct gtaagggtgg aagtggtctc
     5761 tttttcgagt gtttggtgag tggtttccgc aaccttaacc agtccttgag c
//
LOCUS       DASFSW010000123         5696 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.123, whole genome shotgun sequence.
ACCESSION   DASFSW010000123 DASFSW010000000
VERSION     DASFSW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.123"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1006)
                     /locus_tag="U1099_001736"
     CDS             complement(<1..1006)
                     /locus_tag="U1099_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM4868029.1"
                     /translation="MQASSRSLRLMGTIIETRIWYPQAETILDQVEELLYLYKDRFSA
                     NDLTSELMEVNLNAGVQAVPVADDLYELIKLGKEHSLAQGSFLNITIGPLVQSWRIGF
                     SDAKLPTQEMISEKLQLINPRDIELDDEEQTVYLKKEGMAIDLGALAKGYVADKIVDF
                     LKRIGVEAGLINLGGNVLTFGQAPHNADGYWRIGIQDPQKLRGENALVLKIGEESVVT
                     SGIYERTLTVNGKTYHHILSPETGYPMESQLASLTIVSKQSVDGEIWTTRLFGQEITR
                     IYKMVEETDGIEAVLIGLDGRILVTSGLSEKVLAGEERISDSLGSPSRGGFGARVTSD
                     L"
     gene            complement(993..1937)
                     /locus_tag="U1099_001737"
     CDS             complement(993..1937)
                     /locus_tag="U1099_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEM4868030.1"
                     /translation="MTIYSKIIKKIHEYDTIIIHRHKRPDPDAIGSQVGLQKLLQKNF
                     PEKTIKVTGFEEPNLAWMAGMDVVADQDYQGALVIVTDTANTARVDDDRYTQGDFLIK
                     IDHHPNEEPYGDLLWVNTEASSCSEMITELAIQSQLELDGDIARLLYAGIVGDTGRFL
                     YPATSSRTFEIVARLRQEDFDFARMSRQMDTIDFKIAKLTGYVYDNLEVDEHGAARVI
                     LTQDILEKYGVTDADTSFIVGAPGRIGIVQSWGIFVEQTDGNYRVRLRSKYLPINEIA
                     KRHDGGGHPLASGANSYSLSENEQIYQEIQEVVRNASK"
     gene            2032..2493
                     /locus_tag="U1099_001738"
     CDS             2032..2493
                     /locus_tag="U1099_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEM4868031.1"
                     /translation="MALAKIVYASMTGNTEEIADIVASKLEELGLEVQVHECTTIETE
                     EILDADLIVVASYTYSYGGDGELPDEIVDFYADLADLDLTGKVYGVCGSGDTFYDDFC
                     SAVDDFDVMLGSRGATKGAENVKVDLAAEDEDIVNLEQFATDLVAKLDTMN"
     gene            2633..2902
                     /gene="rpsN"
                     /locus_tag="U1099_001739"
     CDS             2633..2902
                     /gene="rpsN"
                     /locus_tag="U1099_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEM4868032.1"
                     /translation="MAKKSKMARYQRQLELIERYADLRKSLKEKGDYQALRKLPRDSN
                     PNRLKYRDRTDGRPHAYMRKFGVSRITFRELAHLGQLPGVKKASW"
     gene            2984..3262
                     /locus_tag="U1099_001740"
     CDS             2984..3262
                     /locus_tag="U1099_001740"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEM4868033.1"
                     /translation="MNLDCIREQINTIDSQLVELLENRMELVDQVTAYKRATGKPVLD
                     TSRENAVLERVGKLVQKDDYRSAIQATFSDIMAQSRTYQSSKLANHEQ"
     gene            3252..4466
                     /locus_tag="U1099_001741"
     CDS             3252..4466
                     /locus_tag="U1099_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEM4868034.1"
                     /translation="MSNKMKQAIQLLFFSNLIGVGAGIITTLFGKILLGIGDLRSEYF
                     TFLIPFLPLAGGVIVFIYQKWGREVQAGMGLVFKAGQGEKAQISPVLIPLIISTTWLS
                     HLVGASVGREGVAVQLGASLSHWLKKHGFTHLPKDMITKIGMAAGFAGLFQTPLAASF
                     FAIEVLIVGQYSWTSLPYCLVAAFTASTTSHLLGLEKFSHTVATTSFYFTDSFKWLFI
                     ALCFGFIGNLFAWFLAQAKSISTRWLPNPYIKIAIMGVGLTVLLFFFHQGHYTGLGTN
                     LIDASLAGEQVFAFDWLLKLLLTCLCLAAGFQGGEVTPLFAIGASSGVVLAGLLGLPT
                     ELVAALGYCAVFGTATNTLLAPLFISYEVFGANILPYAIPVLAIAYLINRKQTIYGQQ
                     LRKFNNAKKPII"
     gene            complement(4900..5415)
                     /locus_tag="U1099_001742"
     CDS             complement(4900..5415)
                     /locus_tag="U1099_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868035.1"
                     /translation="MKSKRLKLGQIDLQMCKDFDIIQAMDYDFQSKKILNKGRGFAIV
                     LVQIQELTFLIPLRSYMPKGYQLKYKLRPSNRVGYVEGLDLGKSLILEDKKYLLNTNF
                     RLREVTDYYKLMDNDKMIINKLIKVIVDFNKAVSDKDIHKLTDPKRFKFSTFVNYKER
                     LKTISEKDYLK"
BASE COUNT         1594 a         1240 c         1124 g         1738 t
ORIGIN      
        1 caaggtcaga agttactctg gcgccaaatc cccctcttga aggactgccc aagctatcag
       61 aaattctttc ttcaccagca agaactttct cagaaagccc tgaggtcact agaatcctgc
      121 catccagccc aatcagaacc gcttcgatac catcagtttc ttctaccatc ttataaatcc
      181 gagttatttc ttgaccaaac agtcgagtcg tccaaatctc accgtcaaca gattgtttgg
      241 acacaatggt caaactagcc agctgcgact ccattggata gccggtctca ggactcaaaa
      301 tatgatgata ggtctttcca ttcacagtaa gggttctttc gtaaatacca gaggtgacta
      361 ctgattcttc tcctattttt aggacgaggg cattttcacc acgtagtttc tgaggatcct
      421 gtataccaat tcgccaatag ccatctgcat tgtgaggagc ttgaccaaag gtcaggacgt
      481 tgccacccag attaatcagt ccagcctcca cacctattct tttcaagaag tccacaatct
      541 tatctgctac atatccttta gcaagagcac ccagatcaat cgccatacct tcttttttca
      601 aatagactgt ctgctcctca tcatctaatt cgatgtccct tggattgatg agctggagtt
      661 tttcactgat catttcttgg gttggaagtt tggcgtcgct aaatccaatc cgccagcttt
      721 gtacaagggg accaatggta atattcaaaa aagatccctg agccagactg tgttctttgc
      781 ccaatttaat caattcatac aggtcatcgg caacaggaac cgcctgaaca cccgcattga
      841 ggttgacctc cattaattca gaggtcaagt cattggcaga aaaccgatcc ttgtagagat
      901 agagaagctc ttctacctga tccaaaatcg tttctgcctg aggataccat attctggttt
      961 caatgatggt tcccataaga cgaagggagc gactacttgc ttgcattcct cactacctct
     1021 tggatttctt ggtatatttg ctcattttcg gagagtgaat aagagttagc accacttgct
     1081 agtgggtggc caccgccatc atggcgtttg gcaatctcgt tgattggaag atacttactg
     1141 cgcaaacgga ctctgtaatt tccgtcagtt tgttccacaa aaatccccca agattgaacg
     1201 attccaatcc gtcctggcgc acctacgata aaggaagtgt ccgcatcggt caccccgtat
     1261 ttttcgagaa tgtcctgtgt caaaataaca cgcgccgcac catgttcatc cacttccaag
     1321 ttgtcgtaga cgtaacctgt caacttggca atcttgaaat cgatggtatc catctggcga
     1381 gacatccttg caaagtcaaa atcttcttgg cggagacgag caacaatctc aaaggtgcgc
     1441 gagctggttg ccggatagag aaagcgacct gtatcgccca caattcctgc atagagtaga
     1501 cgagcaatat ctccatcgag ttccagttgg ctttggatgg ctagttccgt aatcatttca
     1561 ctgcatgagc tggcttccgt attgacccag agcaggtcac cataaggctc ttcattgggg
     1621 tgatgatcga ttttaatcaa gaaatctcct tgggtatagc ggtcgtcatc cacacgcgcc
     1681 gtattggcag tgtctgtcac gataaccaag gctccttgat aatcttggtc tgcaactacg
     1741 tccattcctg ccatccaagc aagatttggt tcctcaaaac cggttacctt gatggttttt
     1801 tcaggaaaat tcttctgcaa gagtttctgc aagccaactt gactaccaat ggcatccgga
     1861 tctggtcgtt tatgtcggtg aataataatc gtgtcgtatt cgtgaatttt tttaataatt
     1921 ttactgtaaa ttgtcatagc aactcctttt catcatttta gcataaactt ccccttttga
     1981 aaatgatggg agtgtggtat aatgggatta ctatgtaatt tggaggaaat tatggcacta
     2041 gcaaaaatag tttacgctag tatgactggt aacacagaag aaattgctga catcgtcgcc
     2101 agtaagctag aagagttagg tttggaagta caggttcacg aatgtacgac tattgaaacc
     2161 gaagaaatct tggatgcgga cctcatcgtg gtagccagct acacctattc ttacggtgga
     2221 gacggtgagc ttccagatga aattgttgat ttctatgctg acctagctga cttagatttg
     2281 accggtaagg tctacggtgt ttgtggttct ggcgatacct tctatgatga cttctgtagt
     2341 gcagtagatg actttgatgt catgttgggt tcacgtggtg caactaaggg tgccgaaaat
     2401 gttaaggtag atcttgccgc tgaagatgag gatattgtta accttgagca attcgcgaca
     2461 gaccttgttg ctaaactaga tacaatgaat taactgacaa acagactggc ttatcaatag
     2521 gtctagtctg ttttctttta gccttttatt aagattgtac acctattgct tgcaatctta
     2581 atagttttga tatataatta actggttaag ttttttgaaa aaggagaatt ttatggctaa
     2641 aaaatcaaaa atggcacgct atcagagaca gttggagtta atcgagcgct atgcggattt
     2701 acgtaaaagt ctcaaagaaa aaggtgacta tcaagcactt cgaaaattgc ctcgcgactc
     2761 aaatccaaat cgactgaaat accgcgacag gaccgatggc cgtccgcatg cttatatgcg
     2821 aaaattcggt gtgagtcgga ttaccttccg tgaattagcc catcttggtc aacttcctgg
     2881 ggttaaaaaa gcaagttggt aacttcctca aacaaggcta gtattctgcc ttgttttttg
     2941 ctatactaaa aaacaactga aacaatatac ggaggtttat aagatgaatt tggattgcat
     3001 ccgcgaacag attaacacga ttgatagcca attggttgaa ctacttgaaa atcgaatgga
     3061 gttggtcgac caagttacag cctataaacg agcaacaggt aagcctgttt tggataccag
     3121 ccgagaaaat gctgtccttg aaagagttgg aaaattagtt caaaaggatg attaccgttc
     3181 tgccattcaa gcaaccttta gcgatattat ggcccaatca agaacctatc aatcttccaa
     3241 gttagcgaat catgagcaat aagatgaaac aagccatcca actccttttc ttttccaact
     3301 taattggagt cggtgcagga ataatcacca cactttttgg gaaaattcta ctagggattg
     3361 gagacctacg gtctgaatat tttacttttc taatcccctt tctgccactt gcaggggggg
     3421 tgattgtttt catttatcaa aaatggggaa gggaagtcca agcaggtatg ggcttagtct
     3481 tcaaagctgg acaaggggag aaagcgcaaa tttctccagt tctcattcca ctcatcatct
     3541 ctacaacttg gctcagtcat cttgtcggtg cttccgttgg tcgcgaaggt gtagctgtcc
     3601 aacttggagc cagtctctca cattggttaa aaaaacatgg tttcacacac ttgcccaaag
     3661 acatgataac aaagattggt atggccgcag gttttgcagg gctatttcaa acaccgttgg
     3721 ctgctagttt ctttgccatt gaggtattaa tcgttggcca atattcttgg actagtctcc
     3781 cctattgtct agttgccgct ttcacggctt ctactacttc ccatttattg ggattagaga
     3841 aattttcgca taccgtcgct actacctcat tctattttac agatagtttt aaatggctat
     3901 ttattgccct ttgcttcggt ttcatcggca atttatttgc ttggtttctg gcgcaagcaa
     3961 aaagcatctc aactcgatgg cttccaaatc cttacattaa aatagctatc atgggagttg
     4021 gactgactgt tctactattt ttcttccatc aaggtcatta tactggtcta ggaaccaatt
     4081 tgatcgatgc cagtttagct ggggaacagg tgtttgcttt cgattggcta ctaaaactcc
     4141 tactaacctg tctttgtttg gctgcaggtt ttcaaggggg cgaagttact ccgctctttg
     4201 cgattggagc aagttctggg gttgttttag caggattact aggtctacca actgaacttg
     4261 ttgcagccct tgggtattgt gccgtatttg gaacagccac caatacctta ctggctcctc
     4321 tcttcattag ttatgaagta ttcggcgcta atatcctgcc ctatgctatt cctgtacttg
     4381 ccatagctta tcttataaac agaaaacaga ctatttatgg acagcagttg aggaaattca
     4441 ataatgcaaa aaagcctatc atctaggctt tttcgtatac tcttcatctc ccctgcagga
     4501 attttatagc aaaacacgtt gttgtatgtt ttaaaaaata cgctgatttt agctattttt
     4561 gttatatttt tgacctatct atactcattt tttaaaactt ttggggcgaa tttggggcag
     4621 acgcactaaa aaaacactcc ataaatcaac ttctcaagac ctctggacaa ggtctttttt
     4681 tgtccgcaaa aacggcaatt tttaaaaacg tccgagataa aagtaagtat ttttctatca
     4741 aattttgaaa aaacgctatt tttttgaaat tcgggtaaca aaaaaagccc ccagcaaacg
     4801 ctgaggggtt ctgaaataca atgatagcgg ttgggcggcg tatccaacat ctcaggtact
     4861 ctctcgagtg cgtcaggagc ctttctgacc tcgctatcac tattttaaat aatctttctc
     4921 ggaaatagtt ttcagacgct ctttataatt gacaaaggtt gaaaacttaa aacgtttagg
     4981 gtctgttagt ttgtgaatat ccttatccga tacagcttta ttaaaatcaa caataacttt
     5041 aataagttta ttgataatca ttttatcgtt atccatcaac ttatagtagt ctgtcacctc
     5101 tcgcaatcga aagttagtat tcaaaagata tttcttatct tccaaaataa gagatttccc
     5161 taaatccaat ccttcgacat accctaccct gtttgacggg cgcaatttat actttaactg
     5221 ataccctttt ggcatataac tacgcagggg aataaggaaa gtcaattctt ggatttgcac
     5281 caaaacaatc gcaaaaccac gacctttatt caagattttc ttactttgaa aatcataatc
     5341 catagcttgg ataatatcaa aatctttaca catttgtaaa tcaatttgac caagtttcaa
     5401 tctcttactc ttcatctaca cctaccacaa aaaaatccta ccaacgggat attggcagga
     5461 cgcatttcca atgggatact tctgttaacg agcgcgtccc tccgccccct cattgccaca
     5521 aggcatgggt ttaacgacaa gcgatgtcgg aacatagaaa ggaggtgttt aaaccgtttc
     5581 tgttcacagt tattattcca cacatcccta tattttgtca agaaaaatct cgcaaccatt
     5641 attgacaaaa tggctatttt gaacgaatag actactgtat tctactgttt cctact
//
LOCUS       DASFSW010000124         5687 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.124, whole genome shotgun sequence.
ACCESSION   DASFSW010000124 DASFSW010000000
VERSION     DASFSW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.124"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(32..1654)
                     /locus_tag="U1099_001743"
     CDS             complement(32..1654)
                     /locus_tag="U1099_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM4868036.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEDERVIDVVIMGNEQLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEDLHYQNMSELTNGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSINWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKIFVGNYDFWKQSSELAAKLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIIPSSRKYPFINFKSEREIGNDLLTVENLKVVIDGETI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSQSYLPKDNTR
                     DFDTNESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKAFKESIIFASHDHEFIQTLANHIIVI
                     SKNGVIDRIDETYDEFLENAEVQAKVQELWKA"
     gene            complement(1719..2591)
                     /locus_tag="U1099_001744"
     CDS             complement(1719..2591)
                     /locus_tag="U1099_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM4868037.1"
                     /translation="MKERIKDFISVTLGSIVMAIGFNSFFLENNIVSGGVGGLAIALN
                     ALLGWSPSDFVLYCNIPLLIICWFFLGKSVFIKTVYGAIIYPLCIKLTAGLPNLTENP
                     LLAAIFGGIILGFGLGLVFLGNSSTGGTGILIQFIHKYTPLSLGLTMAIIDGIIVGLG
                     FVAFDTDTVMYSIIALMTITYIVNRMMSGTQSSRNVMIISQKSEEIKDYITKVADRGV
                     TELPIIGGFTGVDKRMLMTTISIPEMQKLETAVLEIDETAFMVVMPASQVRGRGFSLQ
                     KDHKHYDEDILIPM"
     misc_binding    2693..2899
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2946..3971
                     /gene="trpS"
                     /locus_tag="U1099_001745"
     CDS             2946..3971
                     /gene="trpS"
                     /locus_tag="U1099_001745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEM4868038.1"
                     /translation="MSKPIILTGDRPTGKLHIGHYVGSLKNRVLLQDAGQHELFVFLA
                     DQQALTDHAKEPQKIVESVGNVALDYLAAGLDPAKTTIFIQSQIPELAELTMYYMNLV
                     SVARLERNPTVKTEIAQKGFGEGIPAGFLVYPVSQAADITAFKANFVPVGNDQKPMIE
                     QTREIVRSFNHAYQTDILVEPEGIYPENEAAGRLPGLDGNAKMSKSLGNGIYLADDMD
                     TLKKKVMSMYTDPNHIRVEDPGKIEGNMVFHYLDVFGREEDKAEIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELGPIRERRIEFAQDMGQVYAMLEAGSQKARQVAATTLDQVKSA
                     MGINYFK"
     gene            4203..5684
                     /gene="guaB"
                     /locus_tag="U1099_001746"
     CDS             4203..5684
                     /gene="guaB"
                     /locus_tag="U1099_001746"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEM4868039.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPHDIDLKTQLAPNLTL
                     NLPIISAAMDTVTDSKMAIAMARAGGLGVIHKNMSIAEQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEAEKLMATYRISGVPIVETLENRKLVGIITNRDMRFISDYSQPISTNMT
                     SDALVTAPVGTDLATAEAILHKHRIEKLPLVDENGRLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIKEIREHFPTR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAGVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGHAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKQGSKDRYFQASVNEANKLVPEGIEGRVAYKGSVADMIFQMVGGLRSGMGY
                     VGAGNLTELHENAQFIEMSGAGLKESHPHDVQITNEAPNYSVQ"
BASE COUNT         1705 a         1082 c         1246 g         1654 t
ORIGIN      
        1 gaggctgggc aaaagtccca gcctcttttt attatgcttt ccaaagttct tgtacttttg
       61 cttgtacttc agcattttcc aagaattcat cataagtttc gtcgattcgg tcgataacac
      121 cgtttttaga aatgacgatg atatggtttg ctaaagtttg aataaattcg tggtcgtggc
      181 tggcaaagat gatggactct ttaaaagctt tcaaaccatc attcagactg gaaattgatt
      241 ccaagtccaa gtggttggtt ggatcatcta agaccaagac atttgacttg aggagcatga
      301 gttttgacaa catcacgcgc actttttcgc cccctgacaa gacgtttaca ggtttgttaa
      361 cctcatcacc cgagaagagc atacgtccca agaaaccgcg caagaaggta ttgtcatctt
      421 cttccttgct ggcaaattga cggagccaat caaggataga ttcgtttgta tcaaagtcac
      481 gagtattgtc ttttggtaag taggattgac tagtagtgac accccacttg atagtacctt
      541 catattcaat atcgcccata agagcacgaa tgagagcagt tgtttggatg tcgttttgac
      601 caataagagc agtcttgtca cctggacgca ggataaagct gatattgtca agaatcgttt
      661 caccatcgat gacaactttc aagttttcaa ctgttaggag gtcattacca atttcacgtt
      721 ctgatttgaa gttgataaat ggatacttac ggctagaagg aataatttcc tccaactcga
      781 ttttgtccag catctttttc cgtgaagtag cttgcttaga cttagaggca ttagcagaga
      841 aacgggcaac gaattcttgt agttctttga ttttttcttc tgctttggca ttacggtcag
      901 cttgtagttt agctgctagt tcactggatt gtttccagaa gtcatagtta ccaacgaaaa
      961 tcttgatttt accgaagtca aggtcggcca tatgggtaca tactttgttt aagaagtggc
     1021 ggtcgtgtga cacgacgata accgtatttt caaagtcaat caagaaatct tcgagccagt
     1081 tgatagattg gatgtccaga ccgttggttg gctcgtccaa aagaagaaca tcaggcttac
     1141 caaagagggc cttggccaag aggaccttga ccttctcacc gttggtcaat tcgctcatgt
     1201 tttggtagtg gaggtcttct gaaatgttga ggttttggag caattgagaa gcttcacttt
     1261 cagcttccca gccaccaagt tcagcaaact caccttctaa ttcagcagca cgaacaccat
     1321 cttcatcaga aaaatcttcc ttcatgtaaa tagcatcttt ttctttcatg atgctgtaaa
     1381 gttgctcatt tcccatgata acaacgtcaa tcacgcgctc atcttcatag tcgaaatggt
     1441 tctgacgtag tacagaaagg cgttcgtctg gtccaagtga aatatgacct gttgatggct
     1501 cgatgtcacc agcaagaatt ttcaaaaatg tagatttgcc agcaccattg gcaccaatca
     1561 aaccgtaggt atttcctgct gtaaatttga tattgacatc gtcaaataat ttgcggtcgc
     1621 taaagcgaag cgacacgtct gatactgtaa gcatagtttc tcctctattt ctgtctatta
     1681 gttttattgt actagaaaga acttgaattt tcaatgaatt acattggaat caaaatatct
     1741 tcatcataat gtttatggtc tttttgaaga ctgaagccac gtccacgcac ctgactcgca
     1801 ggcataacaa ccataaaggc agtttcatca atttctaata ccgctgtctc cagtttctgc
     1861 atttctggaa tagagatggt agtcatcaac atgcgcttgt caacccctgt aaagccacca
     1921 ataattggaa gttctgtcac accgcgatca gcaactttag taatatagtc tttaatttct
     1981 tctgattttt gagaaataat catgacattt cgagaagatt gggtaccaga catcatgcgg
     2041 ttgacaatat aagtaatggt cattagggca ataatagagt acataacagt atcagtatcg
     2101 aaggcaacaa atccaagacc aacgataata ccatcaataa tagccattgt cagacctaat
     2161 gataaagggg tgtacttgtg aataaattga ataagaatac ctgttccacc agtagaagaa
     2221 tttccaagga agacaagccc aagtccaaaa cctaggataa ttccaccgaa aatagcagct
     2281 aggagaggat tttctgtcaa gttgggtaaa cctgctgtca acttgatgca aagagggtag
     2341 ataatagctc catagacagt tttgataaat acagattttc ctaagaaaaa ccaacagatg
     2401 attaataaag ggatgttaca gtagaggaca aaatcagacg gactccaccc cagaagagca
     2461 ttgagtgcga ttgctaaacc tccgacacca ccagatacga tattattctc tagaaaaaaa
     2521 ctgttaaatc caatggccat gacaattgat ccgagtgtga cagagataaa gtcctttata
     2581 cgttctttca tatattctcc ttggttagtg ttataaaagc aatttctatt atccctaatt
     2641 tagacagtat cgtcaaggct ttgtcagctt gactttctaa ttttttgcgg ttataatagg
     2701 aatcaatcag aagagtagta agtggataat ttttagagag tttgtggttg gtgtgaacaa
     2761 acaagagtcg cttatgaatg gactgagtga atagaatctg aataaataag gattcggcta
     2821 gcaggcctta tgtgcaaaaa gatgtaagct tatttaatgc ttataagtga ggtggcaccg
     2881 tgtccttgac gccctcgcac agttttctgt gcgtgggctt ttattatgta aaaggagaac
     2941 attttatgag taaaccgatt atcctaacag gagaccgtcc aacaggcaaa ttacatattg
     3001 gacattatgt tggttcgttg aaaaatcgtg ttttattaca ggatgcaggt caacatgaac
     3061 tttttgtctt tttagcagac caacaagcgt tgacagatca tgcaaaggaa ccgcaaaaga
     3121 ttgttgaatc cgttggaaac gtagcactag attatctagc ggcaggactt gatccagcta
     3181 agacaacgat tttcatccag agtcaaattc ctgaattggc agagttgacc atgtactaca
     3241 tgaacttggt atctgttgca cgtctagaac gcaatccgac tgtaaaaacc gagattgctc
     3301 agaagggatt tggcgaagga attccagctg gtttcttggt ctatcctgtg tcacaagcgg
     3361 cggatattac agccttcaag gcaaactttg ttccagtagg aaatgaccag aaacctatga
     3421 ttgagcagac tcgtgaaatc gttcgtagct ttaaccatgc ttaccagact gatattttgg
     3481 tagagccgga gggcatttat cctgaaaatg aggcagctgg tcgcttgcca ggtttggacg
     3541 gcaatgccaa gatgtctaag tcacttggaa atggtattta tctggcagat gacatggata
     3601 cactcaagaa gaaagtcatg tccatgtaca ctgatcccaa ccacatccgt gttgaagacc
     3661 caggtaagat tgaaggaaac atggttttcc actatcttga cgtttttggt cgcgaagaag
     3721 ataaggctga gattgaggct atgaaggagc actatcaacg tggtggtttg ggcgatgtga
     3781 agaccaaacg ttatttgctg gacattttag agagagaatt aggaccaatc cgtgagcgtc
     3841 gtatcgaatt tgcacaggat atgggacagg tctatgcaat gttggaggca ggtagccaaa
     3901 aagctcgcca agttgctgcg acaactcttg accaagtgaa atctgctatg ggaattaact
     3961 attttaaata aggttaacaa tgaaagagca atgttgagaa aatgttgctt ttttcattta
     4021 ttttacgacg tagcaaaaaa caaacgttcg aattttatat ataaaactga atagaatttt
     4081 ctgaattttt agtaatgatg atattaaagc gaataatgat tgacaaatga tttcaaaatg
     4141 ttatcataat taaaatacaa atagcaatca tgctacatat taaataagaa aagaggaaga
     4201 aaatgtcaaa ctgggacact aaatttttga aaaaaggctt tactttcgac gatgtattac
     4261 ttattccggc tgaaagtcat gtattaccac atgatattga tttaaaaaca caattagcac
     4321 caaatttgac ccttaatctt ccgattattt ctgctgctat ggatacagta acagatagca
     4381 aaatggctat cgctatggcg cgtgcgggtg gtttgggcgt catccataag aacatgtcta
     4441 tcgcggagca ggctgatgag gtgcgcaagg taaaacgttc tgaaaatgga gtcattatcg
     4501 atccattctt cttaacacca gaacacacca ttgctgaagc tgagaaattg atggctactt
     4561 atcgtatttc cggtgtgcca atcgttgaaa cattagaaaa tcgtaagttg gtcggtatta
     4621 tcaccaatcg cgatatgcgt ttcatttcag attactcaca gccaatttcg accaacatga
     4681 ccagcgatgc tttggtaaca gcgccagttg gaacagatct tgcgactgcg gaggctattc
     4741 ttcacaagca tcgtattgaa aaattgccat tggttgatga aaatggtcgt ttgtcaggct
     4801 tgattactat caaagatatt gagaaagtga ttgagttccc aaatgctgcg aaggatgaat
     4861 ttggtcgtct cttggttgcg ggtgctgttg gtgttacatc tgatactttt gaacgtgcag
     4921 aagctctctt tgaagcaggt gcagatgcga ttgtcatcga tacagctcat ggtcattcag
     4981 ctggtgtttt gcgtaagatc aaagaaatcc gcgaacactt cccaactcgt accttgattg
     5041 ccggcaacat tgcgactgca gagggtgcac gtgcccttta tgaagcaggg gttgatgtcg
     5101 ttaaagttgg tattggtcca ggttccatct gtacgactcg cgtaatcgca ggtgtaggag
     5161 ttcctcaggt gacggctatt tacgatgcgg caggtgttgc gcgtgaatat ggtaaaacga
     5221 ttatcgctga tggtggtatc aaatattctg gagatattgt caaggcactt gcagcgggtg
     5281 gtcatgccgt tatgcttggt tctatgtttg caggaacgga tgaagcacca ggtgagacag
     5341 aaatcttcca aggtcgtaag ttcaagacct atcgcggtat gggctctatc gctgccatga
     5401 aacaaggttc aaaagaccgt tacttccaag catctgtcaa tgaagccaac aaacttgttc
     5461 cagaaggaat tgaaggacgt gtagcctata aaggttcagt tgcagatatg attttccaaa
     5521 tggttggtgg acttcgctca ggtatgggct atgtaggtgc tggtaacttg actgaattgc
     5581 atgaaaatgc acaattcatc gaaatgtcag gtgctggttt gaaagaaagc catccacacg
     5641 atgttcaaat cacaaacgaa gctccaaact attcagttca gtaaaac
//
LOCUS       DASFSW010000125         5664 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.125, whole genome shotgun sequence.
ACCESSION   DASFSW010000125 DASFSW010000000
VERSION     DASFSW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.125"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            1..804
                     /locus_tag="U1099_001747"
                     /pseudo
     CDS             1..804
                     /locus_tag="U1099_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            885..1466
                     /locus_tag="U1099_001748"
     CDS             885..1466
                     /locus_tag="U1099_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEM4868040.1"
                     /translation="MELNVFAGQEKSELSMIEVARAILEERGRDNEMYFNDLVNEIQN
                     YLEKSNSEIRAALPTFYSDLNVDGSFIPLGENKWGLRSWYAIDEIDEEVITLEEDDED
                     APKRKKKRVNAFMDGDDDAIDYGHDDPEDEDNYPGSVSSEYDDENPDDEKDEVESYDS
                     EINEIIPDDELDEEDVDLGEDDDEYSDEEVVDE"
     gene            1659..3263
                     /locus_tag="U1099_001749"
     CDS             1659..3263
                     /locus_tag="U1099_001749"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEM4868041.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKDKIKRAARTTDADVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNMQAQNMDQIVCDHLKLDVPPADMTE
                     WTAMVNKVLNLQKKVKIALVGKYVELQDAYISVVEALKHSGYANDAAIELDWVNANDL
                     TAENVAERLGQAQGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCVEFA
                     RHVLGLEGANSFELDPETKYPIIDIMRDQIGVEDLGGTLRLGLYPSKLKRGSKAAAAY
                     DNQEVVQRRHRHRYEFNNDFREQFEKAGFVFSGVSPDNRLVEIVEIPENKFFVACQYH
                     PELQSRPNRPEGLYTAFVSAAIENEK"
     gene            3555..3640
                     /locus_tag="U1099_001750"
     tRNA            3555..3640
                     /locus_tag="U1099_001750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3589..3591,aa:Leu,seq:aag)
     gene            3875..4756
                     /locus_tag="U1099_001751"
     CDS             3875..4756
                     /locus_tag="U1099_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEM4868042.1"
                     /translation="MPLVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVARNLIANLIESMNITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKAKEVVELAHAKGISVEAEVGTIGGEEDGIVGAGELAPIEDAV
                     AMVATGIDFLAAGIGNIHGPYPANWEGLNLDHLRKLTEAVPGFPIVLHGGSGIPDAQI
                     QEAIKLGVAKVNVNTECQIAFANATRKFAAAYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSANKA"
     gene            5151..5339
                     /locus_tag="U1099_001752"
     CDS             5151..5339
                     /locus_tag="U1099_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEM4868043.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
BASE COUNT         1874 a         1032 c         1205 g         1553 t
ORIGIN      
        1 gccaattatc acaaatttgt catcacttct accaaaacta ttgtccagat tcctttaaac
       61 atcggcgtaa tgttggctta gccaaagttt cagatgactc actccttgtc ttgctcttgt
      121 tacaggccga actaggcata acgtcacagc gtcatttcta tagcatctgc cacctctttc
      181 cttgtggtca gttactagaa agaagccgtt tcaatcgtcg gtcaaaacaa ttgatttggc
      241 tcgtccaatt aatccgaaaa gctatgagtg aaatgcttcc atctgataaa ctagccatta
      301 tagatagttt tcctttgcca acctgttcgc aatcacagag ctaccatttt taaaggatta
      361 gccgatattg gctacaacgc ctctaaacat ctttggttct acggcttcaa ggttcatatg
      421 ctggtgacct tatctggttt tattctgaac tatgttgtca cacctgcctc tgttcacgat
      481 atcaaggtgg tttatgaact attagaaggg tgtaagcaat cggttatcct aggtgattta
      541 ggctacctga gcagtgaact caagaaggac ttagaacaac aaggttacca tctatggacg
      601 ccatttcgac aaaacatggc agaggctgaa gaacataaca attggaaagt aatgaccatg
      661 agacgaacta ttgaaacacg tttttctgaa ctgtgtcgtc tctttgacat cgaacataca
      721 ttagctagaa gcctatccgg actacagttg caaattgaac aaattattct agctcataac
      781 ttacgctatt ttgaaatgaa ctagcaccac gggtaaagat tatctttgac tatttgcctt
      841 ttttagcgta aaatagaaag gaaagacaaa aaaggagaac agccttggaa cttaacgtat
      901 ttgcaggaca agaaaaaagc gaactttcca tgattgaagt agcccgtgct attttagaag
      961 aacgtggacg tgacaacgaa atgtatttca acgacttggt caatgaaatt caaaactacc
     1021 ttgaaaaatc aaacagcgag attcgcgcag cccttccaac cttctattct gatttgaatg
     1081 tggatggaag cttcattcca cttggcgaaa acaaatgggg cttacgttct tggtacgcaa
     1141 tcgatgagat cgacgaagaa gtgatcactc ttgaagaaga tgacgaagat gcaccaaaac
     1201 gcaagaagaa acgtgtcaat gccttcatgg acggtgatga tgatgcgatt gactatggac
     1261 atgatgatcc ggaagatgag gataactatc cgggtagtgt atcatcagaa tacgatgatg
     1321 aaaatccaga tgacgaaaaa gatgaagttg agtcctatga ttcagaaatc aacgaaatta
     1381 tcccagatga cgaattggat gaagaggatg ttgatttagg tgaagatgac gacgagtatt
     1441 cagatgaaga ggtcgtcgac gaataagaat tatatcccca tagattctta aaaataattt
     1501 gacaagacta ctgaattacg ataggatatt atcgggcacc tcttttgagg tcggagctcc
     1561 ctagcgatag ggagctattt ttgttttttc ttcaagttaa caggaagtat ccccacaaat
     1621 tgcttcctat tagcctgaat cgtagaaaag gagtttgcat gacaaagtat atttttgtaa
     1681 caggtggcgt tgtttcctct atcggcaaag ggattgtagc agccagcctg ggtcgtttat
     1741 tgaagaaccg aggtctcaag gtaacaatcc aaaaatttga tccatacatc aatattgacc
     1801 cgggaacaat gagtccgtat caacacggtg aagtttttgt gacggacgac ggcgcagaaa
     1861 ccgacttgga ccttgggcat tatgagcgtt tcatcgacat taacctgaac aaatattcaa
     1921 atgttacaac aggtaaaatt tatagcgaag tcctccgtaa agagcgtaaa ggggaatact
     1981 taggggcaac agttcaagtt attccacaca ttacagatgc tttgaaagac aaaatcaagc
     2041 gtgcagctcg tactacagat gcagatgtca tcattacgga agtgggtggt acagtaggtg
     2101 atattgaaag cttgccattc cttgaagcgc ttcgtcagat gaaggcagat gtcggttcag
     2161 acaatgttat gtatatccat acaacactct tgccttatct caaagcagca ggcgaaatga
     2221 aaactaagcc aacacaacat tctgtaaaag agttgcgtgg tctaggtatt cagcccaaca
     2281 tgttggtaat tcggacagag cagcctgctg gacaaggaat taaaaataaa ctggcacaat
     2341 tctgtgatgt tgcaccagaa gcagttattg agtccttgga tgtcgaacat ctttaccaaa
     2401 tcccattaaa tatgcaagcg cagaacatgg atcaaattgt ttgcgatcac ttgaagctcg
     2461 acgtacctcc agcagatatg acagagtgga cagcgatggt aaacaaggtt cttaatctcc
     2521 agaaaaaagt aaaaatcgct ttagttggta agtatgtaga attacaagat gcctatattt
     2581 ctgtagttga agctctaaaa cactcaggat atgcaaatga tgctgctatc gaattggact
     2641 gggtcaatgc aaatgatttg acagcagaaa acgtagctga acgtctagga caagcacaag
     2701 gaattatcgt tcctggtggc tttggacaac gtggtacaga agggaaaata caagccattc
     2761 gttatgctcg tgagaatgac gtgccaatgc taggggtctg cttgggaatg cagctgacct
     2821 gtgtagaatt tgctcgtcac gttctcggtt tagaaggtgc taacagcttt gaattagacc
     2881 cagaaaccaa atatccaatt attgacatca tgcgtgatca aattggagtg gaagatttag
     2941 gaggaactct ccgtttaggt ctttatccaa gcaagctcaa acgtggctcc aaggctgcag
     3001 ctgcttatga taatcaagaa gttgtacaac gtcgccatcg tcatcgttat gagtttaata
     3061 atgacttccg tgaacaattt gaaaaagcag gttttgtttt ctcaggtgtg tcaccagaca
     3121 accgtttggt agaaattgtt gaaattccag aaaataaatt cttcgttgcc tgccaatacc
     3181 atccagaact acaatctcgt cctaatcgcc ctgaaggttt gtatacagcc tttgtttcag
     3241 cagctataga gaatgaaaaa taagtataac aatttataga aggagattgg tattattcaa
     3301 tcttctttta tattttatat aaagaaacga aaaaggtgtc agtattttat agaagaaaat
     3361 acgaaatacc acccccatat ggcatcattg tctagatggt tttaagcatt atattttaag
     3421 cattatatag tagaatattg caaaagaatc agctaaatca atacgttttg gcaaaaaagg
     3481 taaaaataaa aaataaaatt caaaaaaagt gttgacaagg ctagttccta atgatatact
     3541 agttcttgtg ttaagcgggg atggcggaat tggcagacgc gcaggactaa ggatcctgtg
     3601 accgctttag gtcgtgtggg ttcaagtccc actctccgca tagtggacta gctaaggcta
     3661 gtccttttgc tttgtctaga taagtgcaga gcacgatata tgctagttca taaattacaa
     3721 gaatgatacg aaacaggcat tattatggct ttcatgaaaa gatatgtgaa agttttctca
     3781 aaactagacg gtttcaacta gcattttcaa aataaaaatg gtagaataga agagtatgag
     3841 gtgcaacctc aaaaaatatt aggaggctat cgaaatgcca ttagtttcag cagaaaaatt
     3901 tgtccaagca gcgcgtgaca acggttatgc agttggtgga tttaacacaa acaaccttga
     3961 gtggactcaa gctatcttgc gtgcagcaga agcaaaacaa gctccagttc ttatccaaac
     4021 ttctatgggt gcagctaagt acatgggtgg ttacaaagta gctcgtaact tgattgcaaa
     4081 cttgattgaa tcaatgaaca ttacagttcc agttgctatt caccttgacc acggtcacta
     4141 cgaagatgca cttgagtgta tcgaagttgg ttacacttct atcatgtttg acggttcaca
     4201 ccttccagta gaagaaaacc ttgcaaaagc aaaagaagtt gtagaattgg cgcacgctaa
     4261 aggtatctca gttgaagctg aagttggtac tatcggtggt gaagaagatg gtatcgtagg
     4321 tgctggtgaa ttggctccaa ttgaagacgc agtagcaatg gttgcaactg gtattgactt
     4381 cttggcagca ggtatcggta acatccacgg tccataccca gcaaactggg aaggtcttaa
     4441 ccttgaccac ttgcgtaaat tgactgaagc tgtaccaggt ttcccaatcg tattgcacgg
     4501 tggttcaggt attccagatg cacaaatcca agaagcaatc aaattgggtg ttgctaaagt
     4561 taacgtaaac accgagtgcc aaatcgcatt tgcaaacgca actcgtaagt ttgcagcagc
     4621 ttatgaagca aacgaagcag aatacgataa gaaaaaactc tttgacccac gtaaattctt
     4681 ggcagacggc gtgaaagcta tccaagcatc tgttgaagag cgtatcgacg tattcggttc
     4741 agcgaacaaa gcttaatgta actgcgaaga aatctcagat ttcaagcaaa caaagcataa
     4801 gtttcttaga ttaaaccaaa aaggattttc tttcgagaat cctttttatt ataaatagtt
     4861 gattaagttt gatctagttc tttgccatga agaaatctac gatagaagtg agcagagaac
     4921 agttaaaata taaaacgctc caaagttttc atgactgtaa taacttagag aacgaagaat
     4981 tatggatggg gcgcaaactg tagtattaaa atatttacct atgaatatag atgctttaaa
     5041 aaaataaaaa tgaaaataat tcttgccaag tccaatcttt tctgttagaa tagtatggta
     5101 tgtggtgcta gcacatccga tatatttgat caaatgagga ggaagataca atggctaaag
     5161 tatgttattt cactggtcgt aagactgtat caggtaacaa ccgttcacac gctatgaacc
     5221 aaactaaacg cgcagttaaa ccaaaccttc aaaaagtaac tgttttgatt gatggtaaac
     5281 ctaaaaaagt ttgggcttca gcacgtgcac taaaatcagg caaagttgaa cgcgtataat
     5341 aacaaagtcg ttagaaaaat ctagcgactt ttgtttttgg aaaaaatagc ctagatataa
     5401 agtcggtgag gagcaaattc ggccactaca taataaacat gaaagaatat cagctatata
     5461 gctcttcaca ttgctattga aaagttaaca aagaaggatc gttgtcaaaa ataaactgtg
     5521 tattgatgtg gaagattttt ccaagttcga acatttaaac ttggattgac attaggagtt
     5581 gtacgagaga gcatccgtac gggttctaca agaaaggaat cacgaactgg aagaatagag
     5641 agtgtttaaa aaaatccaaa aaat
//
LOCUS       DASFSW010000126         5627 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.126, whole genome shotgun sequence.
ACCESSION   DASFSW010000126 DASFSW010000000
VERSION     DASFSW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.126"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            152..1300
                     /locus_tag="U1099_001753"
     CDS             152..1300
                     /locus_tag="U1099_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEM4868044.1"
                     /translation="MKHVFIIGSRGLPAKYGGFETFVQQLVSHQQSEKICYHVACLSD
                     TSHHQHSTYLGADCFTINPPKLGPARVIAYDMMAITYALKIIKEQQMERPIFYILGNT
                     IGGFIVPFAKQIHSVGGTLFVNPDGLEWKRAKWAKPVQRYLKFSEKMMVKYADLIIAD
                     NEGIEDYIQSEYSATNTRFIAYGTDLSPTKLTKESEQVRQYFTNWNIKEKEFYLIIGR
                     FVPENNYVTAIKEFMASNTRRDLVIVANHEGSAYFQKLKDETGFISDKRIKFVGTVYD
                     QELLKYLRQECRAYIHGHEVGGTNPSLLEALAQTNENLVLGVDFNRKVALEGARYWSK
                     ESGSLSQLINQIDENAEAVELGEIAKKHMKDEYTWEKIVQEYEELFLQ"
     gene            1297..2226
                     /locus_tag="U1099_001754"
     CDS             1297..2226
                     /locus_tag="U1099_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4868045.1"
                     /translation="MKVNILMSTYNGQQFLAEQIRSIQEQTYTDWTLFIRDDGSSDRT
                     REIIKDFVEQDQRIHFIDVETDENLGVIKSFHRLIHYDTADFYFFSDQDDVWLPNKLE
                     VSLKKAQAYPTNLPLMVYMDLKVVNQNLEVMAESMVKSQSHHANTELVQELTENTVTG
                     GVAMINHHLAEMWQVTEDILMHDWYLALLASALGKLVFIDKPGELYRQHADNVLGART
                     LSKRFKKWIRPHILFAVYWDLIKNSQKQARHLLQMPLSQSNRELIEAFVTIMDKPMLE
                     RFRILRKYGLRKNKTFHTLVFTTLIVTKFAYKE"
     gene            2229..3035
                     /locus_tag="U1099_001755"
     CDS             2229..3035
                     /locus_tag="U1099_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4868046.1"
                     /translation="MNLLNKENQILLREMIKTDFKLRYQGSLIGHLWSILKPMLLFTI
                     MYLVFVRFLRFDDGTPHYAVGLLLGMVTWNFFTEATNMGMMSIVSRGDLLRKLNFSKE
                     IIVFSSVAGAAINYAINLMVVFVFAAINGVPFTWSILIIIPLFFELALLATGIAFILS
                     SLFVRFRDIGPIWEVFLQAGLYATPIIYSLTFILQRGQVTFAKLMMLNPVAQIIQDLR
                     HFIVYSGNTTVSELIENPVIVAIPYILPVVVYLLGITVFRKNAKKFAEIL"
     gene            3035..4249
                     /locus_tag="U1099_001756"
     CDS             3035..4249
                     /locus_tag="U1099_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868047.1"
                     /translation="MVKNNNIAVKVEHVSKSFKLPTESSQSLRTTLVNLFKGIKGYVE
                     YNVLQDISFEVEKGDFFGIVGRNGSGKSTLLKILSQIYVPERGTVAVDGKLVSFIELG
                     VGFNPELTGKENVYLNGAMLGFTAEEIDAMYDDIVEFAELSEFMNQKLKNYSSGMQVR
                     LAFSVAIKAQGDILILDEVLAVGDEAFQRKCNDYFLERKKSGKTTILVTHDMGAVKKY
                     CNKALLIEKGYVKALGEPDDVANQYSFDNVLASISEVTENEEPLHQSDIVKDLQINLV
                     SKNQIEPDESIEIEFRYTVLEDIETHVAFTFLDLERHFDVYNDNSMDLKTFGKGEKFF
                     RVKCKLPSINQAKLKMAVSVRNSNKQPLLFAKTSDTPAIFISRKFSTDNIAEEDAATG
                     FIQRNSQWELLD"
     gene            4270..>5627
                     /locus_tag="U1099_001757"
     CDS             4270..>5627
                     /locus_tag="U1099_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis protein F"
                     /protein_id="HEM4868048.1"
                     /translation="MSRLLVYVHYNKYNVVSEYIYFQLKSIRSIYSDIVFVSNSHVSK
                     DIVQYLQSERLIDFFIQRDNIGYDFAAWKEGLNQVTFYQYDSVTLMNDTCFGPLWDLE
                     DYYSQFDSDVDVDFWGMTNHLETKIDSVVVPEHLQSYFMVFKKQILQSQAFVGFWSSV
                     SELTDIQDVIKLYESQLTKILLSEGYSYKCVLDTSIYCKTLENSNITLEYPEVILKNN
                     VPFIKIKSFTEYPDRIYSLLHLIRMKTKYPVELIERHLRQIIIPGTSFIPPIRVSQTT
                     ETVRSSTSVLLHVHIESVSIFEEYIEELCKISDRCQLLITLPETDFSNKCSIVERYLS
                     TYKLRAQIAKLTDELHFFEIVNNYMGDAKYLAHVTVKQTKEIKYSVEDIIDRHQLRKM
                     FFTSFDAVISNFESQSNLAVVIPDLTTNQRYDRQSLREGNPELIRQLNILYESLVRTK
                     KV"
BASE COUNT         1864 a          836 c         1129 g         1798 t
ORIGIN      
        1 tttcttatta actcaaaatt tgtttttatc tatactgtgg agtcagaggc gtagaaaaag
       61 aaaagccttt ataaccaaac taggatagaa ttaggaaaaa aaactaaaaa atggtataat
      121 gaaaagaact gcaatttgat ggagaggatt catgaaacac gtttttatta tcggtagtcg
      181 tggactgcct gcaaaatatg gtggctttga gacctttgtc caacaattgg tcagtcacca
      241 gcaatctgag aagatttgtt atcatgtggc ttgtttatcg gatacaagtc atcaccaaca
      301 ttcgacctac ctaggtgcag attgcttcac cattaatcca ccaaaacttg gaccggctcg
      361 tgtgattgct tatgatatga tggcgatcac ttatgcactt aaaatcatta aggaacagca
      421 gatggagcga ccaattttct atatcttggg aaataccata ggtggcttca tagtaccttt
      481 tgcgaaacag attcactctg ttggaggtac cttgtttgtc aatccagatg gtttggaatg
      541 gaaacgtgcc aagtgggcta agccagtcca aaggtatcta aaattttctg aaaaaatgat
      601 ggtcaaatat gctgatttga ttattgctga taatgaggga attgaggatt atattcaatc
      661 tgaatactcg gctaccaata cacgctttat tgcctatggt acagatttaa gtccgactaa
      721 gttaacaaaa gaatctgaac aagttcgcca atattttaca aattggaaca ttaaagagaa
      781 agaattttat ctgattatcg gtcgttttgt acccgagaat aactatgtga cagcaattaa
      841 ggagtttatg gcaagcaaca cgagacgtga tttagtcatc gttgcaaacc atgaaggctc
      901 tgcctatttt caaaaattga aagatgagac aggctttata tccgataagc ggattaagtt
      961 cgtaggaaca gtatatgacc aagaactact taagtatctg cgccaagaat gtcgagctta
     1021 tattcatgga catgaagttg gaggaaccaa tcctagtctc ctagaagcgc tggcgcaaac
     1081 caatgaaaat ttggttttag gtgttgattt taaccgcaaa gttgccctag aaggtgctcg
     1141 gtattggagt aaggagtcag gtagcctgag tcaattaatt aaccaaattg atgagaatgc
     1201 tgaagcggtg gaattaggag aaattgcgaa aaaacacatg aaggatgaat acacctggga
     1261 aaaaatcgtc caagagtacg aggaattatt tttacaatga aagtaaacat actcatgtcg
     1321 acctacaatg gtcaacagtt cttagctgag caaattcgct ctattcaaga acagacttac
     1381 acagactgga cactgttcat tcgtgatgat ggttccagtg atcgaactag agagattatt
     1441 aaagattttg ttgaacaaga tcagcgaatt cacttcatag atgttgagac agacgaaaat
     1501 ctcggtgtca tcaaaagttt tcataggcta attcattatg atactgctga tttctatttt
     1561 ttcagtgacc aagatgatgt ttggctacca aataaactgg aagttagcct gaaaaaagca
     1621 caagcctatc caacaaattt gccattgatg gtttatatgg acctcaaagt tgtcaatcaa
     1681 aatctagaag ttatggcaga aagcatggta aaatctcaat ctcatcatgc caatacggaa
     1741 ttggtgcaag agttgactga gaatactgta actggcggtg tcgccatgat taatcatcat
     1801 cttgctgaaa tgtggcaagt aactgaggat attttgatgc atgactggta tttagctttg
     1861 ttggcctcgg ctttgggtaa actggttttt attgacaagc ctggcgaact ctatcgccag
     1921 catgcggata atgttttggg agctagaacc ctttctaaac gatttaaaaa atggattcgt
     1981 cctcacattt tatttgcagt ttattgggac ttgattaaaa atagtcaaaa acaagcccgg
     2041 catttattac aaatgcccct ttctcaatca aatagagaat taatagaagc ctttgtaacc
     2101 attatggata aaccaatgct tgaacgattc aggattttga gaaaatatgg tttaagaaaa
     2161 aacaaaacct ttcatacatt agtattcaca acactgattg tgacaaaatt tgcatataag
     2221 gaataacaat gaatttatta aataaagaaa atcaaatact cttaagagag atgattaaaa
     2281 cagattttaa attacgttac caaggttctc tcattgggca tctttggtcg attttgaaac
     2341 cgatgttact ttttacaatt atgtatttag tatttgttcg ttttttgcgc tttgatgatg
     2401 gaactcctca ttatgcagta gggttattat taggtatggt aacctggaac ttttttacag
     2461 aagcaactaa tatggggatg atgtctattg tttcacgcgg agatttattg cggaagttga
     2521 atttttctaa agaaattatt gtattttctt cagttgcagg agctgcaatt aattacgcca
     2581 ttaacttaat ggttgtgttt gtatttgctg ctattaacgg ggttcccttt acttggtcca
     2641 ttctaattat aatcccattg ttttttgaat tggcattact agcaacaggt atagctttca
     2701 ttttatcctc tttatttgta agatttagag atattggtcc aatttgggaa gtgttcttac
     2761 aggctggatt gtatgcaaca ccaattattt attcactaac attcatttta cagcgtgggc
     2821 aagtaacatt tgctaaattg atgatgttga accctgttgc acaaattatt caagacttga
     2881 gacattttat tgtttattct gggaatacta cagttagtga attgattgaa aatcctgtta
     2941 ttgtggcaat tccgtatatt cttccagtcg ttgtttacct attgggtata actgtgttta
     3001 gaaaaaatgc caaaaagttt gcggagattt tgtaatggta aaaaataata atattgctgt
     3061 gaaagtagaa catgttagca agagttttaa gcttccaacg gagagtagtc agagtctaag
     3121 aactacttta gtcaatttat ttaaagggat taaaggctac gtagagtata atgttttgca
     3181 ggatatcagt ttcgaagttg aaaaaggaga tttttttgga attgttgggc gcaatggttc
     3241 tggtaagtct acactgttaa aaattttatc tcaaatatat gttcctgaaa gagggactgt
     3301 agctgtggat ggtaaattag tttcttttat tgaacttggt gttgggttta atccggaact
     3361 aactggtaag gaaaatgtat acctaaacgg tgcgatgtta ggatttactg cagaagaaat
     3421 tgatgcaatg tatgatgata ttgttgaatt tgcggaattg agtgaattca tgaatcaaaa
     3481 gttgaaaaac tattctagtg gtatgcaggt acgtttagcc ttttcagttg ccattaaagc
     3541 tcaaggagat attctgatac ttgatgaggt tctagcagtc ggtgatgagg cctttcagcg
     3601 taaatgtaat gattacttcc tagagcgtaa aaaaagtgga aaaacaacca ttcttgtcac
     3661 ccatgatatg ggagctgtga aaaagtattg taataaagct cttcttatag agaaaggata
     3721 cgtcaaggca ttaggagaac ctgatgatgt agctaatcaa tacagttttg ataatgtgct
     3781 tgcctcaata agcgaagtta cagaaaacga agaacctctg catcagagtg atattgtaaa
     3841 agatttgcag attaacctgg tctcaaaaaa tcaaattgaa ccagatgaat caattgaaat
     3901 cgagtttaga tatacggttt tagaagatat tgaaacacat gttgcattta catttttaga
     3961 tttagaacgt cattttgatg tatataacga caattctatg gatttaaaaa catttggtaa
     4021 aggcgagaag tttttccgag tgaaatgtaa gctaccttca attaatcagg caaaattaaa
     4081 aatggcggtg tcagtacgaa atagtaataa gcagccgtta ttatttgcca agacatctga
     4141 cacaccagct atttttatta gtaggaagtt tagtactgat aatattgcag aggaagacgc
     4201 agcaaccggg tttattcaga gaaatagcca atgggagctg ctagattaat aaaatataac
     4261 ggaggcagaa tgtcgcgttt gttggtatat gttcactata ataaatataa tgttgttagt
     4321 gaatatattt attttcaatt aaagtcgata cgaagtattt attcagacat agtttttgtt
     4381 tctaacagcc atgtttcaaa ggacatagta caatatttac agtcggaacg attgattgat
     4441 ttttttatcc aaagagataa tattggttat gattttgcgg catggaaaga agggctgaat
     4501 caagtaacat tttatcaata tgattcggta actctgatga atgatacatg tttcggcccc
     4561 ctctgggacc tagaggatta ttatagtcaa tttgacagtg atgttgatgt agatttttgg
     4621 gggatgacca atcacttaga gacaaaaata gatagtgtag ttgtcccgga acatttacag
     4681 tcatatttta tggtatttaa aaaacaaatc ttgcagagtc aagcatttgt tggtttttgg
     4741 tcatcggtaa gtgagttaac agatattcaa gatgttataa aattatatga gagccaattg
     4801 acaaaaatac ttttatcaga gggttatagc tataagtgtg tattagatac gtccatttat
     4861 tgtaaaaccc ttgaaaatag caacattaca ttagaatatc cagaagttat acttaaaaat
     4921 aatgttccat tcataaaaat aaagagtttt accgaatatc cagacagaat atactcttta
     4981 ttacatctta taaggatgaa aacgaaatat ccagttgaat taatcgagag gcatttgagg
     5041 cagataatta ttccgggtac gagttttatt cctccaatac gggtttcgca aacgacggag
     5101 accgttcgtt cttcgacgtc ggtacttctt catgttcaca tagaatcagt gtctattttt
     5161 gaagagtata ttgaagagtt atgcaaaata tctgataggt gtcagttact aataacattg
     5221 ccagaaactg atttttcaaa taagtgtagt atagttgaga gatatttgtc tacttacaaa
     5281 ttgcgagccc aaattgcaaa actcacagat gaattgcatt tttttgaaat agtaaacaac
     5341 tatatgggag atgccaaata tcttgctcat gtaactgtca aacaaacaaa ggaaataaaa
     5401 tattctgtag aggatattat tgacaggcat cagttgagga aaatgttttt tacatcattt
     5461 gatgcagtga tttctaattt tgaatcacaa tctaatttgg ctgtagttat tccagactta
     5521 acaactaatc aaagatatga tagacaaagt cttcgagagg gcaatccaga acttattcgt
     5581 caattgaaca tactttatga atcacttgtg agaactaaga aagttga
//
LOCUS       DASFSW010000127         5618 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.127, whole genome shotgun sequence.
ACCESSION   DASFSW010000127 DASFSW010000000
VERSION     DASFSW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.127"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1163
                     /locus_tag="U1099_001758"
     CDS             <1..1163
                     /locus_tag="U1099_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4868049.1"
                     /translation="YIQTEFKANPITGKRQRYKSYLNRWGKPFDTEKEAYNELCRVRA
                     EHHDKFEYADYDISYKAFMNSVFLPYYKQTVQGLTYRTAMVHFELFIEEFGDTKLRNI
                     TPRMCEQFRVKIIANYSPNYAKQLWCRFKQSLGYAERLEYIKSFPCKSLDNPKGKRPE
                     TKFWTYEEFLKVINQFDLTDYEDHLRFVTVWLYYMTGVRVSEGFSLIWSDFNAKKKTL
                     HVQSTLEAIGKGQYLRKLQTKTEAGKRYISLDDETVRILSEWRKIQVANSKDNYILSR
                     FGEPMVKSTLSRMLKRYALKAGVPVITGKGLRHSHDSFMINVLHLDVVALSHRSGRID
                     KATTLNTYSHYYQVDNTIGGQIQEVLEQSGVAKTKSDKTVGYPTIHPTTIEG"
     gene            1350..2099
                     /locus_tag="U1099_001759"
     CDS             1350..2099
                     /locus_tag="U1099_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4868050.1"
                     /translation="MKSYKNYIFDFYGTLVDIRTDENKLEVWNQLTQIYNAFGCSYRP
                     RQLKNAYHRFVEEAERFLAETVSYQYVEIDLETLFIRLLTDAPNENQSTNKPTDLETF
                     GQVVATIFRVLSREKLEAYENTLTSLQTLKDREARLFILSNAQRIFTQAEIEQTGCAD
                     LMEKIYISSDFKMKKPEPAFLQLVLEENNLDVKETVMVGNDLTSDIAIAHELGMDSIL
                     LNTFPYSQEEIDSYRRLGWNFEVVGDIAELL"
     gene            2295..3629
                     /gene="trmFO"
                     /locus_tag="U1099_001760"
     CDS             2295..3629
                     /gene="trmFO"
                     /locus_tag="U1099_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEM4868051.1"
                     /translation="MSQTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKPTPQHK
                     TDKFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRAGEAHRVPAGGAMAMDRENF
                     SQAVTDEIHNHPLIEVIRGEITDIPDDAITVIATGPLTSDALAEKIHALNGGDGFYFY
                     DAAAPIVDSSTINMDLVYLKSRYDKGEAAYLNAPMNKEQFTAFYEALISAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEYKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     FATKKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFRGEEPVIFPQTTAIGALPF
                     YITHTESKHFQPMNVNFGIIKELDGPRIRDKKERYEAIAERSLKDLEEFLTV"
     gene            3706..4542
                     /locus_tag="U1099_001761"
     CDS             3706..4542
                     /locus_tag="U1099_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM4868052.1"
                     /translation="MIKEFNATVRYLESVLESEIDEREFLRISGYSVPMFSRLFSILT
                     DMSLSEYVRLRKLTRAAMDIREGQAKIIDIAFKYGYESSDSFAFAFKQFHQISPSQVR
                     KGLPFKVLLPLQLSLTIQGGKEMDVRIEKKAGFTVAGLLRTAISNEVCPQVWEDLFKN
                     HTYEELAALGSGQSFGVCTLAQADETINYMAAYDVTDSEKATKLGLELLDVHEADYAI
                     FTLIGPVPQSIHAGWRYALETFFPEHGYRHSGAPDFEYYFEGDMSSPDYQMELWIPIV
                     KA"
     gene            4607..5611
                     /locus_tag="U1099_001762"
     CDS             4607..5611
                     /locus_tag="U1099_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEM4868053.1"
                     /translation="MHHNTWKGIILQKQGLLKPQSVQQICQNLNGLQAQFQPYVHIGF
                     RNRMTNEAFQNQDWQASLTRQWSLRRTVHAYLKSEIPLYIHEGRMASVDYLETVSWDG
                     ALPQVKKKFYQVILEALETGPMTREELKILCRAENIGVEEEKQVFNAWGGLLRYMVER
                     GEIYQEYGVKRFHRLRDFQPLPKKEAELEIARRYFTGFGPVSLADARYYFKENKSTVL
                     KWMEQLDLKTVEVAGEERFYLGELEEAELPDCLFVAGFDQLLLGYEKKANPFFNPKYI
                     RNIYTLTGIVKPVVFYKGRFVATWKRDKGTILLDIFEDITQQEEKELEHYRQMFEKIL
                     "
BASE COUNT         1758 a         1023 c         1241 g         1596 t
ORIGIN      
        1 tatacataca aacagaattt aaagcaaatc ctataactgg caaaagacaa agatataaaa
       61 gttatttaaa caggtggggt aaaccgtttg atactgagaa agaagcgtat aatgaattgt
      121 gcagagttcg tgcagagcat catgataaat ttgaatatgc agattatgat atttcttata
      181 aggcattcat gaacagtgta ttcttaccat actataaaca aacagttcaa gggctgactt
      241 atcgaactgc aatggttcat tttgaattat ttattgagga atttggtgat accaaattga
      301 gaaacataac accaagaatg tgtgagcagt ttcgagttaa gattattgcc aattattcac
      361 ctaactacgc aaaacagctg tggtgtaggt ttaaacaatc attaggttat gctgagcggt
      421 tagagtatat aaaatcgttt ccatgtaaat cattggataa cccaaaaggg aaaagaccag
      481 aaacaaaatt ctggacatac gaagaatttt taaaagtcat aaaccagttt gacctaactg
      541 attatgaaga ccacttaaga ttcgtaacag tttggttata ctatatgacc ggagttcgag
      601 taagtgaagg attctcattg atttggtccg atttcaatgc taagaaaaaa acacttcatg
      661 tccaatcaac actagaagcg attggaaaag gacaatatct aagaaaatta cagactaaaa
      721 cagaggcagg gaagcgttat atttcattgg atgatgaaac tgttcggata ttgtctgaat
      781 ggagaaagat tcaagttgcg aattcaaagg ataattatat tctttcacgt tttggtgaac
      841 caatggtaaa aagtacctta tccagaatgt taaaaagata tgcccttaaa gcaggtgttc
      901 cagtgattac aggaaaagga ttaagacact cacatgattc gttcatgata aatgtcttac
      961 atttagatgt tgtagcatta tcacatcgat caggaagaat tgataaagca actacattaa
     1021 acacctattc acattattat caagtagaca acacaattgg tggacaaatt caggaagtgt
     1081 tagaacaaag tggcgtggca aaaacaaaat cagataaaac tgtaggatac cccaccatac
     1141 accccaccac gatagaaggt tagttttgaa aagtattgat aatacaggga aaaattgaat
     1201 gaatgcaact gttactctta aataatagag tttccaatca aaataaaaac ctatcactta
     1261 atgtggtagg ttttttcgtt ctattgttgc aaacgttttc gttgtggggt ataatataaa
     1321 aaatgatgtc taaagattag gagaaagaca tgaaaagtta caaaaactat atcttcgatt
     1381 tttatggtac attggttgat attcgtacag atgagaataa actagaggtc tggaatcagc
     1441 taacacagat ttacaatgct tttggttgtt cctatcgacc gcgccagttg aaaaatgcct
     1501 atcatcgttt tgtagaagag gcagagagat ttctcgcgga aacagtgagt tatcagtatg
     1561 tcgaaattga tttagaaaca ctcttcattc gcctattaac ggatgcacca aatgaaaacc
     1621 aaagtaccaa taagccgact gatttagaga cttttgggca agtagtagcc acgattttcc
     1681 gcgtgctttc acgtgaaaag ttagaggctt atgaaaatac cctaactagt ctacagacat
     1741 taaaggatag agaagcgcga ttatttattc tctccaatgc tcaacgaatt tttactcagg
     1801 cagaaattga acagacaggt tgtgctgatt tgatggaaaa aatttatatc tcctctgact
     1861 ttaagatgaa gaagcctgaa ccagcatttc ttcaactggt tttagaggaa aataatcttg
     1921 atgttaagga aacggttatg gttggtaatg atttgacatc cgatatagct attgctcatg
     1981 aattgggaat ggattccatt ttgctgaata ctttcccgta tagtcaggaa gaaatcgact
     2041 cttataggag acttggttgg aattttgagg ttgttggaga tatagcggag ctattgtaaa
     2101 taagcgatag aacagtaaag aatatacata aaatatatta tgtaaaaagg tgaaactact
     2161 tttatttcaa tgaaggtagt tttgtctttt ctagccattt tctggtatac tagataggta
     2221 taaaatttag aaaggttctg ttttggggta ttcccaaaca gtagtcagat attctgacta
     2281 gaggtattag tttcatgtct caaacccaca ttaacgttat cggagctggc ttggctggct
     2341 cagaagctgc ttatcagatt gccaaacgtg gcattcctgt caaactttac gagatgcgtg
     2401 gtgtcaagcc gactcctcag cacaaaacgg acaagtttgc ggaattggtt tgttccaatt
     2461 cattccgtgg tgatagtttg accaacgctg tcggtcttct caaggaagaa atgcgtcgct
     2521 tggattccat tatcatgcga gctggtgagg ctcaccgtgt gcctgctggc ggtgctatgg
     2581 ccatggaccg tgagaatttc tcccaagcgg tcacagatga gattcacaat cacccactga
     2641 ttgaggtcat tcgcggtgag attacggaca ttcctgacga tgctattaca gttatcgcga
     2701 cggggccttt gacatctgac gccttggcgg agaagattca cgcactcaat ggtggcgacg
     2761 gtttttactt ctacgatgcg gcagcaccga ttgtcgatag ttcgaccatt aacatggact
     2821 tggtttatct caagtcccgt tatgataagg gagaggctgc ctatctcaat gctccgatga
     2881 acaaggagca gtttaccgct ttctatgagg ccttgatttc agccgaggaa gcaccgctca
     2941 attcctttga aaaagaaaaa tattttgaag gctgtatgcc cattgaagtc atggccaaac
     3001 gtgggatcaa aaccatgttg tatggtccga tgaagccagt cggtctggaa tacccagaag
     3061 actacaaggg acctcgtgac ggtgaataca agacacccta tgctgttgtc caactccgtc
     3121 aggacaacgc agcaggtagc ctttacaata ttgttggctt ccagactcac cttaagtggg
     3181 gcgagcagaa gcgggtcttc cagatgattc caggccttga aaatgctgaa tttgtccgtt
     3241 atggtgtcat gcaccgcaat tcctacatgg attcgccaaa cctattggaa cagacctttg
     3301 cgaccaagaa aaatccaaat cttttctttg caggtcaaat gacaggagta gaaggctatg
     3361 ttgagtctgc cgcttctggc ttagtggccg gtatcaatgc tgttcgtcgc ttccgaggcg
     3421 aagagccggt catcttccca cagaccacag ccatcggtgc tctgccattc tacatcaccc
     3481 acacagaaag caagcacttc cagcctatga atgtcaactt tggcatcatc aaggagctgg
     3541 atggaccacg tatccgcgat aagaaggagc gttatgaagc aatagcagag cgttccctca
     3601 aggacttgga agagtttttg acggtttaag ccgatagttt tccgacgaaa agtatcggga
     3661 aaacttggat ggtagacggt aaactgatag ggaaagggga agttgatgat aaaagagttc
     3721 aatgcaaccg tacgttacct agagtccgtt ttagaaagcg aaatcgatga acgggaattt
     3781 ttgcgcatat caggatattc cgttcccatg tttagtcgtc tcttttcgat tctgacagat
     3841 atgtccttgt cagaatacgt tcggttacga aagttgacgc gagctgctat ggacattcgt
     3901 gaaggacagg caaagattat agatatagcc tttaaatatg ggtatgaatc ttcggattct
     3961 tttgcctttg ccttcaaaca atttcatcag atttctccta gtcaagtgcg taaggggttg
     4021 ccttttaagg tcctcttacc cttgcaacta tcattaacca tccaaggagg taaagaaatg
     4081 gatgttcgta ttgaaaagaa ggctggtttt acagttgctg gacttcttcg cacagcaatc
     4141 agtaacgaag tgtgtccgca ggtatgggaa gacttgttca agaaccatac ttatgaagag
     4201 ttagctgctc taggaagtgg gcagtccttt ggagtttgta cattagctca agcggacgag
     4261 acgattaatt atatggctgc ttatgatgtc acagatagtg aaaaagcgac taagttaggc
     4321 ttagaacttc ttgatgttca cgaagctgac tatgctattt ttaccctaat aggccctgtt
     4381 cctcaatcta ttcatgcagg atggcgttat gcgctagaaa ctttttttcc tgaacacggc
     4441 tatcgccatt caggtgcacc agattttgag tactattttg aaggagacat gagttcccca
     4501 gactaccaaa tggaactctg gattcccatt gtgaaagcat aatgtacagg acaccttcgg
     4561 gtgtcttttg tgttatactg ggattagaaa gcggaggaga aatacgatgc accacaatac
     4621 ttggaaggga attattctgc agaaacaagg tctgttgaaa cctcagtctg ttcagcaaat
     4681 ctgccaaaat ctcaatggtt tgcaggcaca atttcagccc tatgtacata tcggttttcg
     4741 taatcgaatg accaatgaag catttcaaaa tcaggactgg caagcctcac tgacacgcca
     4801 atggtcactt cgacggacag ttcatgccta cctcaaatcg gaaattcctc tctatattca
     4861 tgaagggaga atggcaagcg ttgattatct tgaaacagta agttgggatg gtgctctacc
     4921 acaggttaag aaaaaatttt atcaagttat tttggaagcc ttagaaactg gccctatgac
     4981 tcgtgaagag ttgaaaattc tttgccgagc tgagaacatt ggagtagaag aagagaaaca
     5041 agttttcaat gcatggggtg gtctccttcg ttatatggtt gaacgaggag aaatttacca
     5101 agagtatggg gtaaaaaggt ttcatcggtt acgtgacttt caaccactac caaagaaaga
     5161 agcagagctg gaaatcgctc gacggtattt tacaggtttt gggccagtca gtttagcgga
     5221 tgctcgttat tatttcaagg aaaataaatc tacggtccta aaatggatgg agcaattaga
     5281 tttaaaaacc gttgaggtag caggagaaga acgcttttat ctgggagaat tagaagaagc
     5341 agaactccct gactgcctct tcgttgcagg atttgaccaa cttttgctag gctatgaaaa
     5401 gaaagctaat ccctttttta atcctaaata catccgtaat atctatactt tgacgggtat
     5461 tgtcaaacct gttgtatttt ataaagggcg atttgtggca acctggaaac gagataaagg
     5521 tactattttg ctagatattt ttgaagatat cacacaacaa gaagaaaaag aattggagca
     5581 ttaccgccaa atgtttgaga aaattctgtg acattaac
//
LOCUS       DASFSW010000128         5496 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.128, whole genome shotgun sequence.
ACCESSION   DASFSW010000128 DASFSW010000000
VERSION     DASFSW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.128"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            9..1571
                     /locus_tag="U1099_001763"
     CDS             9..1571
                     /locus_tag="U1099_001763"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEM4868054.1"
                     /translation="MRVIEFLDTTLRDGEQTPGVNFSIKEKVTIAKQLEKWGISAIEA
                     GFPAASPDSFEAVRQIAAAMTKTAVTGLARSVKSDIDACYEALKDAKYPQIHVFIATS
                     PIHREFKLQKTKEEILAQITEHVSYARERFEVVEFSPEDATRTELDYLLEVVQTAVDA
                     GATYINIPDTVGFTTPQHYGEIFRYLTTNVKSDREIIFSPHCHNDLGMAVANTLSAIK
                     NGAGRVEGTINGIGERAGNAALEEVAVALEIRKDFYDVTSPIVLKETLNTSELVSRFS
                     GIAIPRNKAVVGGNAFSHESGIHQDGVLKNPLTYEIITPELVGVKKNSLPLGKLSGRH
                     AFVEKLKELDLYFEEGDVASLFACFKKLADKKEKITDADIRALVAGTEISNRDGFQFK
                     DLRLDSQSDGSIQAQVTFINQDEEEVVVVESGKGSVEAVFNAIDQFFHQEISLERYHI
                     DAITDGIDAQARVLVAVENKATETIFNASGIDFDVVKASAIAYIHANVMVQKENSGQI
                     DKKLSEKDLPHI"
     gene            1580..2632
                     /gene="leuB"
                     /locus_tag="U1099_001764"
     CDS             1580..2632
                     /gene="leuB"
                     /locus_tag="U1099_001764"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEM4868055.1"
                     /translation="MTKKIVALAGDGIGPEIMEAGLAVLEAVAGQVGFDYEIEERAFG
                     GAGIDAAGHPLPNATLQACRQADAILLAAIGSPQYDDAAVRPEQGLLQLRKELGLFAN
                     IRPVKIFDSLKDYSPLKADRLDGVDLVMVRELIGGIYFGEHILETDQASDSNTYQAEE
                     IERVVRSAFDLAQKRQKKVTSIDKQNVLATSKLWRKVADEVAKDYPDVTLEHQLVDSA
                     AMLLITNPSRFDVLVTENLFGDILSDEASVLTGTLGVLPSASHAVAGPSLYEPIHGSA
                     PDIAGQGIANPVSMILSVAMMLEESFDLVQAGQTIRQAVEAVFAEGIFTKDLGGTAST
                     KEMTEAIIAQIEREEI"
     gene            2629..4020
                     /gene="leuC"
                     /locus_tag="U1099_001765"
     CDS             2629..4020
                     /gene="leuC"
                     /locus_tag="U1099_001765"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEM4868056.1"
                     /translation="MSGKSIFDKLWDRHVITGQEGQPQLLYVDQHYIHEVTSPQAFDG
                     LREAGRQVRRPELTFATFDHNVPTVDIHNIRDVISKAQMDALAKNIEDFGIPHAAHGS
                     EHQGIVHMVGPETGRTQPGKFIVCGDSHTATHGAFGAIAFGIGTSEVEHVLATQTIWQ
                     VKPKRLLVEFVGQAQRGVYAKDFILALIARYGVDCGLGHVVEFAGPVIDRLSMEERMT
                     ICNMSIEFGSKMGIMAPDQTTFDYLRGRECAPADFDAAVADWKELYSDADTVYDKHLV
                     LDVSDLAPMVTWGTTPAMGVSFDEIFPAIRDHNDERAYAYMDLAPGQKAADIPVGYVF
                     LGSCTNARLSDLQLAARIVKGRKIAEQVTAIVVPGSRPVKRAAEKLGLDKIFMEAGFE
                     WRDPGCSMCLGMNPDKVPAGVHCASTSNRNFEDRQGFGAKTHLCSPAMAAAAALYGRF
                     VDTRQLDILKEGV"
     gene            4020..4613
                     /gene="leuD"
                     /locus_tag="U1099_001766"
     CDS             4020..4613
                     /gene="leuD"
                     /locus_tag="U1099_001766"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEM4868057.1"
                     /translation="MHQFTTWTGTTVPLMNDNIDTDQLLPKQFLKLIDKKGFGKYLLY
                     AWRYLDDNYTDNPDFILNQPDYQGASILISGDNFGAGSSREHAAWALADYGFKVIIAG
                     SFGDIHYNNDLNNGILPIIQPKEVRDQLAQLAPDQEITVDLADQLIRTPFGEFPFDIE
                     QDWKHKLLNGLDDIGITLQYQDLITEYEGNRPSYWQE"
     gene            4676..5461
                     /locus_tag="U1099_001767"
     CDS             4676..5461
                     /locus_tag="U1099_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEM4868058.1"
                     /translation="MTKQIHWDGALSQEGFDIIKGEGGVIVCPTKVGYIIMTADKAGL
                     ERKFDAKERKRNKPGVVLCGSMEELRELAQLTPEIDAFYQKHWDEDILLGCILPWKPE
                     AYAKLQAYGDGREELMTDVRGTSCFVIKFGVAGEQIAKEMWEKEGRMVYASSANPSGK
                     GNRGKVEGIGERIASKVDLIIEADDYVASIQPDKTIETRYEQGVMVSMVDKDGKLIPE
                     QGMDSRSISPCPVVIRKGLDIDKIMMHLSDHFNSWDYRQGEYY"
BASE COUNT         1425 a         1170 c         1483 g         1418 t
ORIGIN      
        1 gtattcttat gcgtgtgatt gaatttttag atacgacttt acgagatggt gaacagacgc
       61 cgggtgtgaa tttttcgatt aaagaaaaag tgaccatcgc caagcagttg gaaaaatggg
      121 ggatttctgc cattgaagcg ggtttcccag cggccagtcc tgattcgttt gaagcggttc
      181 gtcagattgc ggcagcgatg accaagacgg ctgtgacagg tttggcacgg tcagtaaaat
      241 cagatattga tgcttgttat gaggctctga aagatgccaa gtatccgcag atccatgttt
      301 tcattgcgac cagccccatc caccgtgaat ttaaactgca aaagaccaaa gaagaaattt
      361 tggcgcaaat cactgaacat gtcagctatg cacgtgagcg ttttgaggtg gtcgagttct
      421 cgccagagga tgcgacgcgg actgagttgg attatttgct tgaagtggtg cagacagcgg
      481 tagatgctgg tgcgacctac atcaacattc cagatacagt cggctttacg actcctcagc
      541 actacggaga aattttccgt tacttgacaa caaacgtcaa atcggaccgt gagattattt
      601 tcagtccgca ttgccacaat gacctaggta tggcagttgc caatacccta tcggccatta
      661 aaaacggtgc cggtcgtgtc gagggcacca tcaacggtat cggtgagcga gcagggaatg
      721 cggcccttga agaagtagct gttgcccttg aaattcggaa agatttctat gatgtgacta
      781 gtccgattgt tctaaaggaa accctcaaca cttcagaatt ggtctctcgc ttctctggaa
      841 ttgctattcc acgcaataag gcggtggtag gtggcaatgc cttctcacat gaatctggta
      901 tccatcagga tggtgtcttg aaaaatccat tgacttatga aatcattaca cctgagttgg
      961 taggggtcaa gaaaaattct ctgccactgg gtaaactatc tggtcgccat gcttttgtag
     1021 aaaagctgaa agaactagac ctgtattttg aagaaggaga cgttgctagc ttgtttgctt
     1081 gtttcaaaaa actggcagac aagaaggaaa agattacgga tgcggatatt cgtgccttgg
     1141 tagcagggac agaaatcagc aatcgtgatg gtttccaatt taaagatttg cgtttggata
     1201 gtcagtctga cggaagtatt caggctcaag ttaccttcat caatcaggat gaggaagaag
     1261 tggttgttgt ggagtcaggc aagggttcgg ttgaggctgt ctttaatgcc attgatcaat
     1321 tcttccacca agagattagc ttggaacgct accatattga cgccatcacc gatgggattg
     1381 atgcccaggc gcgtgtgctt gtcgctgtag aaaacaaggc aacggaaaca atctttaatg
     1441 cctctggtat tgactttgac gtggtgaagg cttctgccat tgcctatatc catgctaatg
     1501 tcatggtgca aaaagaaaat agtggacaga tagataagaa actatcagag aaggacttgc
     1561 cacacatcta ggagggaata tgacgaaaaa aattgtagcc ttggctggtg atggaatcgg
     1621 tcctgaaatc atggaggctg gcctagcagt cctcgaggct gttgctgggc aagtaggatt
     1681 tgattatgag attgaggaga gagcttttgg tggcgctggg attgatgcgg ctggccatcc
     1741 tctaccaaat gctaccttac aagcctgccg tcaggcggat gcgattttgt tagcagctat
     1801 cggtagccct cagtatgatg atgcagctgt tcgaccagaa caaggtctgc ttcaacttcg
     1861 gaaggaattg ggtctctttg ccaatatccg tccggtgaaa atttttgata gcctgaaaga
     1921 ctattctcct ctgaaggccg accggctgga cggtgtggat ttggtcatgg tacgggagtt
     1981 gataggtggg atttattttg gagagcacat tctggagaca gaccaagcca gcgatagcaa
     2041 tacctatcag gcagaagaga ttgaacgagt tgtccgctct gcctttgacc tagcccaaaa
     2101 aagacaaaaa aaagtcacca gcattgataa gcagaatgta ctggcgacgt caaaattatg
     2161 gcggaaggtg gcggatgaag tggcgaagga ctacccagat gtgaccttgg agcaccagtt
     2221 ggtggatagc gcagccatgc tcttgattac caatcctagt cgatttgacg tcttggtgac
     2281 ggaaaatctt ttcggagata ttttgtcgga tgaggccagc gtattaacag gaacactagg
     2341 ggttctgcct tctgctagtc atgcggtagc aggtcccagt ctctacgaac ctatccatgg
     2401 ttcggcaccg gatattgccg gtcagggcat tgccaatcct gtcagtatga ttctatctgt
     2461 tgccatgatg ctggaagaaa gttttgattt ggtccaagca ggtcagacca ttcgtcaagc
     2521 tgttgaagcg gtctttgcag agggcatttt caccaaggat ctcggtggga ccgcttctac
     2581 caaggagatg acggaggcca ttattgccca gattgagagg gaagagatat gagtggcaaa
     2641 tctatttttg ataagctctg ggaccgccat gtgattacag ggcaggaagg tcagccccag
     2701 ctcctctatg tggaccagca ctatattcat gaggtgacca gtccccaggc ttttgatggt
     2761 ctgcgagagg caggacgtca ggtccgtcgg ccagaattga cctttgcgac ttttgaccac
     2821 aacgtaccaa ccgttgatat tcacaatatc cgtgatgtca tttccaaggc tcagatggat
     2881 gccttggcta agaatataga agatttcggc attccccacg cagcccacgg ttcggaacat
     2941 caggggattg tccacatggt tggaccagaa acaggcagga cccagccagg taagttcatt
     3001 gtctgcggag atagccatac agcgactcac ggagcatttg gagctattgc ctttgggatt
     3061 ggtacttctg aagtggagca cgttctcgct acccagacca tttggcaggt caaacctaag
     3121 cgactcttag tagagtttgt cggtcaggcg caaagaggtg tctatgccaa ggactttatc
     3181 ctggctctga ttgcccgcta cggcgtggat tgtggtttgg gtcatgtggt cgagttcgca
     3241 ggacccgtca tcgaccgact cagcatggaa gagcggatga ctatctgcaa tatgtccatt
     3301 gagttcggct ctaagatggg cattatggct ccagaccaga ccacctttga ctacctgcga
     3361 ggtcgggagt gtgcaccagc ggacttcgat gcggcggtgg cagactggaa ggagctctac
     3421 tcggatgcgg acaccgtcta tgacaagcac ctggtccttg atgtgtcaga cttggcaccc
     3481 atggtcactt ggggaacgac gcctgctatg ggggttagct ttgacgaaat cttcccagcc
     3541 atccgtgacc acaacgacga gcgggcctat gcttacatgg acttagctcc tgggcaaaag
     3601 gctgcggaca ttcccgttgg ctatgtcttc ctaggctcct gtaccaatgc tcgcctcagc
     3661 gatttgcagc tggcagctag gattgttaag gggcggaaaa tagcagagca ggttacggct
     3721 attgtcgtac caggcagtcg ccctgtcaag cgagctgcag aaaagcttgg tctagacaag
     3781 atttttatgg aggctggttt tgagtggcgg gatcctggtt gctccatgtg tctgggcatg
     3841 aatccagaca aggtccctgc gggtgtccac tgcgcctcta caagtaatcg aaactttgaa
     3901 gaccgacagg gctttggggc caagacccac ttgtgtagtc ctgcgatggc tgccgcagct
     3961 gcgctttatg gacgttttgt cgacaccaga cagctagaca ttctcaagga gggagtctaa
     4021 tgcaccaatt taccacatgg acagggacga ctgttccgct catgaatgat aatattgata
     4081 ccgaccagct cttgcccaag cagtttctca agctgattga caaaaagggc tttggcaagt
     4141 atttgctata tgcttggcgg tacttggacg ataactacac ggacaatcct gatttcattc
     4201 tcaaccagcc tgactatcaa ggcgctagta tcctcatatc tggggataac ttcggagcag
     4261 gttcttctag ggaacacgct gcttgggctc tggcagatta tggcttcaag gttatcattg
     4321 caggctcctt tggagatatt cattacaaca atgacttgaa caatggcatt ctgcccatta
     4381 tccagcccaa agaagtcagg gaccaactag ctcagctggc tcctgaccaa gaaatcacgg
     4441 ttgacttggc agatcagttg atacggacgc cttttgggga attcccattt gacatcgaac
     4501 aagactggaa acacaagttg ctcaatggct tagatgatat tgggattacc cttcagtatc
     4561 aggatttgat tactgagtat gaggggaatc gcccaagcta ctggcaagaa taaaataaag
     4621 attagaaaac aagcagatcg ctctgctatg gaacaaaaga aaagaggaaa ctattatgac
     4681 gaaacaaatt cactgggatg gcgcactttc acaagaagga tttgacatta tcaaggggga
     4741 gggaggcgtg attgtctgcc cgactaaggt tggttatatt atcatgactg ccgacaaggc
     4801 tggtttggag cgtaagtttg acgctaaaga gcgtaagcgt aataagccgg gcgtagtcct
     4861 ttgtggtagc atggaggagc ttcgtgagtt ggctcagttg actcctgaga ttgatgcctt
     4921 ctaccaaaaa cattgggatg aggacatttt gttggggtgt atcctacctt ggaaaccaga
     4981 ggcttatgct aagttgcaag cctatggcga tggtcgtgaa gagctgatga ccgacgttcg
     5041 tgggacttct tgctttgtta tcaagtttgg tgtagctggc gagcagattg ccaaggaaat
     5101 gtgggaaaaa gaaggtcgta tggtctatgc gtcatctgct aacccgtcag gtaagggaaa
     5161 tcgtggtaag gtagaaggta tcggtgagcg tatcgcttct aaggtggatt tgattatcga
     5221 ggcggatgac tatgtggcat ctatccagcc agacaagacc attgaaactc gctatgagca
     5281 aggggttatg gtgtctatgg tagacaaaga tggtaaactc attccagaac aaggcatgga
     5341 tagccgttct atcagcccat gcccagttgt tatccgtaag ggtctggaca ttgataaaat
     5401 catgatgcac ttgtctgacc atttcaactc ttgggactac agacaagggg aatattacta
     5461 agatatcgtc atttagttta tcttttatta cgtcgt
//
LOCUS       DASFSW010000129         5474 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.129, whole genome shotgun sequence.
ACCESSION   DASFSW010000129 DASFSW010000000
VERSION     DASFSW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.129"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(109..1386)
                     /locus_tag="U1099_001768"
     CDS             complement(109..1386)
                     /locus_tag="U1099_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEM4868059.1"
                     /translation="MNKQTYKEILTIAFPAMGENLLQMLMGVVDSYLVASIGIVALSG
                     VSLANNILAVYQAVFIALAAAVSSRLAQALGEGGSEGIGGTASESVKFTVFIGLLLGI
                     FSVLAGPIVLASLGAESEVVQAGGLYLILVGGGAPFWGLMASLSAILRTLGEPRFPMY
                     ISLLSNVLNALFSAFAVFVLHAGVAGVAIGTVLSRLIGCSLLWSRLPISLKPWTWSFD
                     KELLRLALPATGERLMMRVGDVVVVALITSLGTATVAGNAIGETLTQFNYMPALGIAT
                     ATIILTAKHRQDKAMVRHIFRTSFGLSLLFMFLVAGVTYFAGPFLISLYTKDIQAVQA
                     SQTVLFYAMLGVPFTAGTLIMTALWQGLGNARLPFYATTIGMWLVRIGIAYLLITFFK
                     IGLSAIWIATILDNAFRAGVLFFQYRYSKQESI"
     gene            complement(1392..2105)
                     /locus_tag="U1099_001769"
     CDS             complement(1392..2105)
                     /locus_tag="U1099_001769"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM4868060.1"
                     /translation="MKKLQVKDLMVTGAFAALYFVCVGLGTLLSLVFDRSGNMMYAPA
                     GAALLAGPVYMLLIAKVGKPGSISLVGANGSGKTSLARYLVGLAEDKKSRISWQGQTL
                     SSRQRLEKTAFVMQDVRLQLFAESVERELTLGRKNRAVDEHLVARFGLSDLLERHPVS
                     LSGGEQQRVMIVASLLADKEIFIFDEPTSGLDLRRMQQVASALVDLKMKNKLVLLISH
                     DEELLNLVCDKIVDIKQLK"
     gene            complement(2106..3767)
                     /locus_tag="U1099_001770"
     CDS             complement(2106..3767)
                     /locus_tag="U1099_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868061.1"
                     /translation="MDKKEMLTRVLIPRLLQSMKHLLPRIAVAVCFAVLGQVVTIAIP
                     VTLVYLAFAALAGNPAPLWTLGILILLALLRGAFRYGEHYFGHYVAFHTLAAYRRLIF
                     AKLRALAPGKLDRQDSGSLLKMIGEDIEALEVFFAHTIAPICTGILVAFGLTVYFGLS
                     SWGLALLALVTYALLAIAIPNRFAQQLEPLLKEQNASRKGYVSYFIESLKSMKDLMQF
                     QQTDARFAKLTQKSQEVNGQERKVAQTNFMQYAVSFLVVGLSIMCFAWLTFDLVGSQS
                     LDLATGVALLVSFTSSFAPFLELSRLPLGFKRAMNAGRNIYALLDEKEAERTGELVEV
                     RVTDIAIEQLDFDYDDRETGLYRNLSVQFEQGGIIGLVGESGAGKSTLMKLIMRWYDW
                     QQGQIRLSGLDSRQVDKAHLQGAFAYVPQVPQIFRQTIRENLLLGRTDVSDETIMDLA
                     EKCHMKERILAAPQGLDTLVEASDFSAGEGQRLELMRALLKNADCYIFDEPTSNLDSL
                     NEARFIQLVKEHCQGMVFLISHRSSTMACADTIFYLEDGQLVKEK"
     gene            complement(3760..5439)
                     /locus_tag="U1099_001771"
                     /pseudo
     CDS             complement(3760..5439)
                     /locus_tag="U1099_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1658 a         1435 c         1104 g         1277 t
ORIGIN      
        1 gtcgactgtt ctctggagat tatatgaaaa agaaattcgc tatttctagc ttgaatatag
       61 tatatagcta caaacttaaa agaaactgaa aattttctta gccttatctt aaattgattc
      121 ttgttttgaa tacctatact gaaaaaatag cacccctgct cggaaggcat tatctaaaat
      181 cgttgcaatc caaatggctg acaaaccgat tttaaaaaat gttataagta aataagctat
      241 gccaatccgc actagccaca taccgatagt tgtggcataa aagggtaatc tagcatttcc
      301 caaaccttgc caaagagctg tcataataag agtgcctgct gtaaagggaa ctcccaacat
      361 ggcataaaat aatactgttt gactagcttg taccgcttga atatccttag tataaagact
      421 gattaggaaa ggccccgcaa aataagtcac accagccacc aaaaacataa acagtaaaga
      481 aaggccaaag cttgttcgaa aaatatgtcg aaccatagcc ttatcctgtc tgtgttttgc
      541 tgtaaggatg attgtagcgg tagcaatccc taaagccggc atatagttaa actgcgtcag
      601 ggtttctcca atcgcatttc cagctaccgt cgctgttcct aagctggtaa tcaacgccac
      661 gactaccaca tctcccaccc gcatcatcaa ccgttcacca gttgctggca gagcaagtct
      721 aagcagttct ttatcaaagg accaggtcca tggttttaaa gaaattggta agcgagacca
      781 aagcaggcta caaccaatca accgcgataa gaccgtgcca atcgcaactc cagctacacc
      841 tgcatgaagc acaaagactg caaatgctga aaatagtgca tttagaacgt ttgagagcag
      901 gctgatatac ataggaaaac gtggttcccc caaggttcgt aagattgcac tcaaacttgc
      961 catgagtccc caaaaaggtg ctcccccacc aaccaatatc aaataaagac ctccagcctg
     1021 taccacttct gactctgccc ccaagctagc aagtactatt ggacctgcca gaactgaaaa
     1081 aattcctaaa aggagaccga taaaaactgt aaacttgaca ctttcactag cagttcctcc
     1141 aatgccttct gaaccccctt cacccaaagc ctgagccaaa cgagaagaca ccgctgctgc
     1201 tagggcaata aaaaccgcct gatagacggc taaaatatta ttagcaaggg aaactccaga
     1261 caaggctacg atgccaatac tggctactag ataagagtct actaccccca ttaacatctg
     1321 gagaaggttt tcccccatgg ctggaaaggc aatggttaaa atttctttat aggtttgttt
     1381 gttcataatc tctatttcaa ctgtttaata tctactattt tatcacaaac cagattcaat
     1441 agctcttcat cgtgagaaat taacaaaacc aacttattct tcatttttaa gtctactaaa
     1501 gcggaagcaa cttgctgcat tcgtctcaaa tccaaaccgc tcgtcggttc atcaaaaatg
     1561 aaaatttcct tatctgccag taagctggcc actatcatca cacgctgttg ttcaccgccc
     1621 gataaactga ccggatgtct ctccaacaaa tccgatagac caaaacgagc taccaaatgt
     1681 tcatcgacag ctctattttt ccttccaaga gtaagttctc gctcaacact ttccgcaaat
     1741 agttggagtc gaacatcctg cataacaaag gctgtttttt ccaatctctg gcgactagat
     1801 aaggtttgac cttgccaaga aatcctactc ttcttatctt ccgctaaacc gaccagataa
     1861 cgagcaaggc ttgtttttcc agaaccattc gctccaacca gactaattga acccggcttc
     1921 ccaaccttgg caatcaagag catatagact ggaccagcta agagagctgc tccagctggt
     1981 gcatacatca tattacccga acgatcaaaa accaagctca acaaggttcc tagaccaaca
     2041 caaacaaagt agagggcagc aaaagcccct gttaccatca gatcttttac ttgtaatttc
     2101 ttcatttatt tttcctttac taattgcccg tcttccaagt agaaaatggt atccgcacaa
     2161 gccatggtcg agcttctatg agaaatcaag aagaccatgc cctgacagtg ttccttaacc
     2221 agttggataa agcgggcttc attgagcgag tctaggttac tagttggctc atcaaagata
     2281 tagcaatccg catttttgag cagggcccgc atgagttcca aacgttgccc ttctcctgct
     2341 gaaaaatcac tggcctccac aagcgtatcc aagccctgcg gtgctgccaa aatccgttct
     2401 ttcatgtggc atttttctgc caaatccatg atggtttcat ccgacacatc cgtccgtccc
     2461 aagaggagat tttcacgaat ggtctggcgg aagatttgtg gcacctgtgg tacataggca
     2521 aaagccccct gcaaatgagc cttatctacc tgccgactat ccaagccaga caggcggatt
     2581 tggccttgct gccaatcata ccaccgcata attagtttca tcaaggtgga ttttcctgca
     2641 ccggactcac cgaccaagcc aataatacca ccctgttcaa actggacaga caggttccga
     2701 tagagccctg tttctcggtc atcgtagtcg aagtccagtt gttcgatagc aatgtccgtc
     2761 actctgacct caacaagctc acctgttcgc tctgcttctt tttcatccaa aagagcatag
     2821 atattgcgac cagcattcat agcccgcttg aaaccaagag gcagacggct gagctccaag
     2881 aagggagcaa aggagctagt gaaagaaacc agcagggcta ctcctgttgc taagtccaaa
     2941 ctctgactac ccaccaagtc gaaggtcagc caggcaaagc acataataga tagtcctacc
     3001 actaaaaagg aaacagcata ctgcatgaag ttggtctggg caacctttct ctcctgacca
     3061 ttgacctctt ggcttttctg tgtcagcttg gcaaagcgag catctgtctg ctggaactgc
     3121 atcaggtcct tcatgctctt gagactttca ataaagtagg acacatagcc cttacggctg
     3181 gcattttgct ccttgagcaa gggctctaac tgctgagcaa aacgatttgg aatggcaatc
     3241 gccaacagag cataggtcac cagagccagc aaagccaaac cccagctgga tagaccaaaa
     3301 tagactgtca aaccaaaagc caccaaaatc cctgtacaaa ttggggcaat agtatgggca
     3361 aagaaaacct ccaaggcctc aatgtcctcc ccaatcatct taagcaggct accgctgtcc
     3421 tgccgatcca atttcccagg agccagggcc cgcagttttg caaaaatcaa acgacggtaa
     3481 gccgccagcg tatgaaaggc cacataatga ccaaagtaat gctcaccata gcggaaggca
     3541 ccacgtaaaa gagctaggag aatgaggatt cccaaggtcc atagcggagc tggattgcca
     3601 gcaagggctg caaaagccag atagaccagc gttactggaa tagcaatggt caccacctgt
     3661 cccaagaccg caaagcaaac cgccaccgca atccgtggca agaggtgctt catggactgc
     3721 aagagtctcg gaatcagcac ccgtgtcagc atttctttct tatccataaa cggcttcctc
     3781 caattctctt tgttgatcga ccagctgacc ataagccaag ctagtctgat aaagcgtggc
     3841 atgattgcct gtcacagctc cttcagcaga caggaagagg atgtcatctg cctgctccac
     3901 ttgtttcatc ttatgtgtaa taataatagc aatcgcatcc ttgaccacca agtcaatgag
     3961 gtcgtaaatc aagccttcat tgtcttgatc gacgctggac gtcacctcgt caaagatata
     4021 aagggagcgt tttgccaaaa ccgcacgggc acagataacc tactggcgtt gaccaggcga
     4081 caagaaagca ccgtcgtcac caacaaccgt atccaaaccg tctggcaaat tcttgacaaa
     4141 ctgcaagacc ccatgctgat ctgcccaagc caagatgtcc tccttagacc agtcacaagc
     4201 catgcgtaaa ttttcataga ttgactggtt gagcaaggtt gactggcccg acacatagag
     4261 gacctgctca ttcagagata actgactgac acctgaaatc tcctactcac ccaagtaaat
     4321 cgctccctta tccgcccgca agcgtcccaa gagcagctgg gccaaggtcg ttttcccctg
     4381 ccctgactca cctgccaaag catagacttt accagccgtc atggtcatag aaatatcttt
     4441 taagactggc ttttctccgt agacaaaggc cagattttcc agtttcaaac tatcaaaaac
     4501 aggcacatgg aaagaatcga tttcctgctc cgctgtcata ctgtccaaga aaccaaaaat
     4561 gcgatccgcc atcttggtat tcatcatcac caagtgcata ccgtagccct gctcccgtat
     4621 gggtgcaaaa aactccgtcg caatgaggac aaagaagagc atactaaaga gggacaaatt
     4681 gcctgctgct aggctattga ccgccacaaa accagacaag ccaatcccca gatacatgac
     4741 cgaatccatg tagcctaccg cttgcaactg aaagctgagt agctccatgg tcgcatcgcg
     4801 gaagtcctcg gcctgctcat tgaaggtctt ttcgtaggtg gcatccgcct gataggaata
     4861 gagggtgttg agccccttga ggtcgtccag gaaaagattg cccacatcca tataggaact
     4921 ccaatagcga ttcataatcc gcttggaccg cttctgcata gcgataatgg aaatgggaat
     4981 cagaggcaaa gccaagatga aaataaccgc acccagcggg aaaaccagaa aaaccagcaa
     5041 aaaaacagtt gcacagttga actgggtccg cagagaagat gccatgtagt aggaatagta
     5101 ggtatccagg ctatcaatcc cctgggaagc gatgttaaag acatccgctg ctgtcgcttt
     5161 tgacgcaaac tgaccgtcct tggccaagag ggcctcaaag aaggactgct tgagcgaatc
     5221 ccgtgcaaac tgggagccca agccctgcaa acgcttggca atcaaggcca caccatatcc
     5281 aaaggcattg agcccgagca agatgaggac aaactgtact aaatcaacct gcccgccctc
     5341 atagtatgtc acaaagccct tagcaatcag gtaaaaggaa ataatccgca tcaaaaactg
     5401 cacccaggat agaaaggctg ccaaataaac cagcttcatc ttgggtttga tgcgttcttt
     5461 gagtcgcttc aaga
//
LOCUS       DASFSW010000130         5429 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.130, whole genome shotgun sequence.
ACCESSION   DASFSW010000130 DASFSW010000000
VERSION     DASFSW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.130"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1171
                     /gene="obgE"
                     /locus_tag="U1099_001772"
     CDS             <1..1171
                     /gene="obgE"
                     /locus_tag="U1099_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEM4868062.1"
                     /translation="VVDEGLRTLMDFRYNRRFKADDGEKGMTKGMHGRGAEDLIVRVP
                     QGTTVRDADTGKIITDLVENGQEFVIAHGGRGGRGNIRFATPKNPAPEISENGEPGEE
                     RNLELELKVLADVGLVGFPSVGKSTLLSVITAAKPKIGAYHFTTIVPNLGMVRTKSGE
                     SFAVADLPGLIEGASQGVGLGTQFLRHIERTRVILHVLDMSASEGRDPYEDYVAINNE
                     LETYNLRLMERPQIIVANKMDMPEAAENLEEFKKKLAANYDEFEELPQIFPISGIAHQ
                     GLKNLLEATAELLEKTPEFLLYEESDFQEEEAYYGFNPDEPEFDISRADDASWVLSGD
                     KLEKLFTMTNFDRDESVMKFARQLRGMGVDEALRARGAKDGDIVRIGKFEFEFVD"
     gene            1545..1709
                     /locus_tag="U1099_001773"
     CDS             1545..1709
                     /locus_tag="U1099_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868063.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNKLNPNRKLRLER
                     ERLAREQEKE"
     gene            2074..>5429
                     /locus_tag="U1099_001774"
     CDS             2074..>5429
                     /locus_tag="U1099_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEM4868064.1"
                     /translation="MRKANKKSFDWYGTKQQFSIRKYHFGAASVLLGVSLVLGAGTQV
                     VKAEEAVTSSEPTIASSVAPASTEAVAEEVDKTNAEATLVPAENTSAVATTSTEVEKA
                     KAVLEQVTSESPLLAGLGQKELAKTEDATLAKAIEDAQTKLAAAKALLADSEATVEQV
                     EAQVAAVKAANEALGNELQKYTVDGLLTAALDTVAPDTTASTLKVGDGEGTLLDRTTT
                     ATPSMSEPNGAAIAPHTLRDKVEATKESGWYTFESYDLNSYNRNQNGSIVGGTAVGAY
                     IRYSLDSDPATSTVLAELVSTKDGEVLESHKLEAGNSVTFSYPKTINAKNSNITLTYD
                     TSTATADIPGSLKFYDDRDAVYSTVVVPAYQVNTTRYVTEDGTVLATYSLQTIAGQTV
                     TSSKVRTFTGYDYVKTTQNAIQGAYPKGTLMLAGVGADKNGNKYYKAIREVVEDNQSV
                     MTLYLLDPTYTGTVDWTGTDTTGFIPLLKTSPTVYTIDRKVYDYNINATILSPYTVDN
                     GFMVFKESATNAQGSKYRVVAQWSGTDETDPTKPEYTGGKYGRIYIGTQVWTTAKELG
                     ITENQWRGTADKFDNNGFVPFGTQQYLRNASVATAVETTHVYKVNPKFGDVIVEYYDT
                     DGKQIVNSVVDTPKSALGTEYNTDVDRRPASLVAADGTVYFYKEVKSDSAKTTGTVVA
                     GTTTVKYVYEKAGSVNVNFVDINGKVIKAPVSDEKDAKPGYNYDTDLDQKLASITFEG
                     KEYKLVPAGDYPVGKVGKGNNLIEVGNNTAKGIDPTTGKIEAGVNKEVTYVYKAVTGS
                     VVVNYKDTEGNVIKDPETDVSDAPVGDAYTTTDKKPNEIITKDGSRYVLVPSKTDGEE
                     NGKVIEGTITVTYVYQKVANWIPEIPNVPETNRPKVPYPFDPTEPDEPIDPTTPGTNG
                     EVPNIPYVPGYTPVDPKDNTPLKPIDPNDPGKGYVPPTPENPGVDTPIPYVPVKKVVT
                     NHVDEEGNPIAPQEEGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPEVKNGTVVVN
                     YVTEDGTVIKEPVTDTPTSPEGTPYDTTDNKPKTITFKGEEYELVRVDGTENGKVVEG
                     ETVVTYVYRKVETPAKKVVTNHVDEEGNPVA"
BASE COUNT         1741 a         1001 c         1215 g         1472 t
ORIGIN      
        1 cgttgtagat gaaggcttgc ggaccttgat ggacttccgt tacaatcgtc gctttaaggc
       61 tgatgatggt gaaaaaggga tgacaaaagg tatgcacggt cgaggagcgg aagatttaat
      121 tgttcgtgta ccgcagggaa caactgtgcg tgacgctgat acaggtaaaa tcattacaga
      181 cctggttgag aatgggcagg aatttgtcat tgcccacggt ggtcgtggtg gtcgtggaaa
      241 tattcgtttt gcgacaccta agaatccagc gcctgagatt tctgaaaatg gagaaccagg
      301 tgaggagcgc aatcttgaat tagaattgaa agtgttggct gatgtaggtc ttgtaggttt
      361 cccatcagtt ggtaagtcaa cgcttctcag tgttattaca gcagctaaac ctaaaattgg
      421 tgcctaccat tttacaacca ttgtgccaaa tttgggaatg gttcggacca aatctggcga
      481 gtcatttgct gtggcagact taccaggttt gattgagggt gctagtcaag gtgttggatt
      541 gggaacgcaa ttccttcgtc acattgagcg gacacgtgtt atcttgcatg ttttggacat
      601 gtcagcaagt gagggtcgtg atccttatga agattacgta gccatcaaca atgaattgga
      661 aacctacaat cttcgtctca tggagcgtcc tcagattatc gttgccaaca aaatggatat
      721 gccagaagcg gcagaaaatc tggaagagtt caagaaaaaa ttggcagcca actatgatga
      781 gtttgaagaa ttgccacaga ttttcccaat ttctggtatt gctcaccaag gtttgaaaaa
      841 tcttttggaa gccacagctg agttgctaga gaaaacgcct gaattcttgc tctacgagga
      901 atcagatttc caagaagaag aggcttacta tggctttaac ccagatgagc cagaatttga
      961 catcagccgt gcagatgacg caagctgggt tctatctggt gacaaactag aaaaactctt
     1021 caccatgacc aacttcgatc gcgacgaatc tgtcatgaaa tttgctcgtc agttacgtgg
     1081 aatgggtgtc gatgaagccc tccgtgcgcg tggtgccaaa gacggcgaca tcgtccgtat
     1141 tggcaagttc gaatttgagt ttgtggatta acgtagcagt ttgactgcaa gttaacttgc
     1201 tagtcaaact atgccaaatc ctatgggacc ttggctagct accttactaa cttcacccaa
     1261 atggtagtat cgaccatttg ggctccgtgt cgtaacgttg gaaacacact taaatcctaa
     1321 aggacttgat gtgatacggt agccgctatg gcggactacc taccaacctt actaattccg
     1381 cacaggcagt ggtatcgact gcctgtactc catatcgtaa cctgtccggt aagttagtct
     1441 agtatgaact tagaaattcg agagcagact atagctttac aaatttcgtt cagttggtga
     1501 cagtcgtttg tgagcaatcg aaaaaaaaat agttggaggt ctgtatgggt gataagccaa
     1561 tatccttcaa ggataaagat ggaaattttg tttcagcagc cgatgtttgg aatgctgaaa
     1621 aattagaaga actctttaac aagcttaatc ccaatcgcaa actccgtttg gaacgtgagc
     1681 gtttggcaag agagcaagaa aaggaataac gtcaaaatct gcttgttgta tcagaaaaca
     1741 ggcagatttt tttaaaattt attgactatt tattattggc tatatgtata agaatacaag
     1801 atatggagct aatatttttg cggtgtcggt aattttatag aaattctcaa aagtgaaatt
     1861 tcttaaaaaa tatgtaaaaa actatgaata tactggtttc ttctcgaatt gaagcgaatt
     1921 cataatgttt ttttgaggaa ttttataata ttacttggca tttaaagtta tttgtagtat
     1981 aatacctcgg atgattgcgg gagttttcaa ggctttgata caaagagtag aaaatttgtg
     2041 taattaaatt aatatttata tgggggattt tttatgcgta aagcaaataa aaaatcattt
     2101 gactggtacg gcacgaaaca acaattttcg attcgtaagt atcattttgg tgcagcaagt
     2161 gttttgcttg gtgtgtcgtt agttttaggt gccggtacac aggttgttaa agctgaggaa
     2221 gctgttactt catcagaacc aactattgcc agtagtgtag cgcctgcttc aacagaagct
     2281 gttgcagaag aagtagataa aacaaatgct gaagctacgc tagtaccagc tgaaaatacg
     2341 agtgcagtag ctacgacttc aacagaagtt gaaaaagcga aagctgttct tgaacaggta
     2401 acatcagaat caccactttt ggctggtctt ggtcaaaaag agttggctaa aactgaagat
     2461 gcaactcttg caaaagctat agaggatgct caaacaaaac ttgcagcagc taaggcactt
     2521 ttggctgact cagaagcaac tgttgagcaa gttgaagcgc aagtcgcagc ggttaaagca
     2581 gccaacgagg cgctagggaa tgaattgcaa aaatacactg tagatggtct cttaacagca
     2641 gcacttgata cagtagcacc tgatacaact gcatcaacat tgaaagttgg tgatggcgaa
     2701 ggtacccttc tagatagaac gacaacagca acaccttcaa tgtctgaacc aaatggtgca
     2761 gcaattgctc ctcatacact tcgagataaa gttgaggcta caaaagaatc tggttggtat
     2821 acttttgaat catatgattt aaattcctac aatcggaacc agaatggttc aattgttggt
     2881 ggaactgctg tcggcgctta catacgttat tccctggact cggatccagc tacatcaact
     2941 gtactagcgg agctagtgag tactaaagat ggagaagtct tagaaagcca taaacttgag
     3001 gctgggaata gtgtgacttt tagttatccg aagacgatta atgcgaagaa tagcaacatt
     3061 actttgactt atgatacttc aacagctacc gctgatattc caggttcgtt gaaattttat
     3121 gatgatagag atgcagtata ttctacagtt gtagtgcctg catatcaagt taatacgact
     3181 cgctatgtta ctgaagatgg cacagttttg gcgacttaca gtcttcaaac tattgcaggt
     3241 cagacggtta cttcatctaa agtacgtact tttactggtt atgactatgt gaaaacaacg
     3301 caaaatgcta ttcaaggtgc ttatcctaaa ggcacactta tgctagctgg tgtcggtgct
     3361 gacaaaaatg gtaataaata ttataaagct attcgtgagg ttgttgaaga taatcaatcc
     3421 gttatgacat tgtacttatt ggatccaact tatacgggta ccgtagattg gactgggact
     3481 gatacgacgg gattcatacc gctgctaaag acatctccaa cagtttatac tattgatcga
     3541 aaagtatatg attacaatat caacgcaact attttatctc cttacacagt tgataatggt
     3601 ttcatggtct tcaaggaaag tgctaccaat gctcaaggta gtaaatatcg tgtcgttgca
     3661 cagtggtcag gcacagatga aactgaccca actaaaccag agtataccgg aggaaagtat
     3721 ggacgtatct atattggtac tcaggtttgg acaactgcaa aagaattggg aattaccgaa
     3781 aaccaatgga gaggtacagc ggataagttt gataataatg gttttgttcc atttggtacc
     3841 caacaatatc tccgaaacgc ttcagtggct acagctgtag agacaaccca tgtctacaaa
     3901 gtaaatccta aattcggtga tgtcattgtt gagtactacg atactgacgg aaaacaaatt
     3961 gtaaattcag ttgtagatac tcctaagtca gctcttggca cagagtataa tacagatgtg
     4021 gaccgtagac cagccagctt ggttgctgct gatgggacag tctacttcta caaagaagtt
     4081 aagtctgatt cagctaagac aaccggtaca gtagttgcag gtacgacaac tgttaagtat
     4141 gtttacgaaa aagctggtag cgttaatgtt aacttcgttg acatcaatgg taaagtaatc
     4201 aaagctcctg tttcagatga aaaagatgcg aaacctggtt acaattatga taccgacttg
     4261 gatcagaaat tagcttccat cacttttgaa ggcaaggaat acaaacttgt tcctgctggt
     4321 gattatccgg ttggtaaagt tggcaaggga aataacttga ttgaagttgg taataatact
     4381 gcgaaaggta ttgacccaac aacaggcaag attgaggctg gtgttaacaa agaagttacc
     4441 tatgtctata aagcagtgac aggttctgta gttgtaaatt acaaagatac agaaggtaat
     4501 gtgattaaag atccagaaac ggatgtgtct gatgcaccgg ttggagatgc ttatactaca
     4561 actgacaaga aaccaaacga aatcatcaca aaagatggat cacgctatgt ccttgttcca
     4621 tctaagacag atggtgagga aaatggtaaa gttatcgaag gaacaatcac agtaacttat
     4681 gtttatcaga aagttgcaaa ctggattcca gagattccaa atgtaccaga aacaaaccgt
     4741 ccaaaagtac cttacccatt tgacccaaca gagccagacg agccaatcga tccaacgaca
     4801 ccaggaacaa atggcgaggt tccaaatatt ccttacgttc caggatatac accggttgat
     4861 cctaaggata acacgccgtt gaaaccaatt gatccaaatg atccaggtaa gggttatgta
     4921 ccaccaacac cagaaaatcc aggtgttgat acaccaattc cttatgttcc agttaaaaaa
     4981 gtcgtaacta accacgttga tgaagaaggt aatcctattg caccgcaaga agagggaaca
     5041 aaaccaaaca aatcaatccc aggttacgag ttcacaggta aaactgttac tgacgaagat
     5101 ggcaacacaa ctcacatcta caagaaaaca ccagaagtta agaatggtac agttgttgtt
     5161 aactatgtaa cagaagatgg cacagttatc aaggaacctg taacagatac accaacttct
     5221 ccagaaggca caccatacga cactacagac aacaaaccta agacaatcac tttcaaaggt
     5281 gaagagtatg aattggttcg tgttgacggt acagaaaatg gtaaagttgt agaaggtgaa
     5341 acagttgtga cttacgttta ccgtaaagtc gaaacacctg ctaagaaagt tgtaactaac
     5401 cacgttgatg aagagggtaa ccctgttgc
//
LOCUS       DASFSW010000131         5315 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.131, whole genome shotgun sequence.
ACCESSION   DASFSW010000131 DASFSW010000000
VERSION     DASFSW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.131"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(19..252)
                     /locus_tag="U1099_001775"
     CDS             complement(19..252)
                     /locus_tag="U1099_001775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868065.1"
                     /translation="MYLRFVLIDISDDGFYHYEIYPENKEEHKQTLVFNPETKDIRVN
                     TFDDASMKYLGKFLQNFKNQDGTYRKELSFGWG"
     gene            complement(257..1477)
                     /locus_tag="U1099_001776"
     CDS             complement(257..1477)
                     /locus_tag="U1099_001776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868066.1"
                     /translation="MNDDILPSLLKEVQDKFEASYGKSDIISSAFAKLKNKKATYSTA
                     NDFAIELGDILAEALGSSVAGDRLPDGKMYYNIGQRLLNDVLGKNFELVSDFTGQVQA
                     NLNKEANIGLKVQVPKFNQDRVDGLVNRLSSEVDFEKVSWLLKEPIVNFTQSIVDDSI
                     KTNADFHAKAGLTPKVVRKESGNCCKWCRAVVGVYNYPDVPKDVWRRHNRCRCTVDYH
                     PGNGKKQNAHSKRWSDPLKNAKIEERKGIGLLVQAGAKNYVRDDSNDELLPKEFIKAE
                     KHAYLTYDRIKNSNQDLEKRKIYSNIWKFKEMNGFSKDDVDKAFDHVFNNVHELNYGK
                     GLFAPDIDMAQSWDRLISGKNIQPHDLILLKHERLEHDYMYVSSKLDYDTAHKKVDEL
                     FNYSEAVDEFKKKG"
     gene            complement(1470..2735)
                     /locus_tag="U1099_001777"
     CDS             complement(1470..2735)
                     /locus_tag="U1099_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM4868067.1"
                     /translation="MSYKGMSYLRKKLALYQLGVKKRYRFYAMADRDNTKSFIIPDNV
                     KSMYNSVMGWTAHSVDALADRIIFREFANDDFEATEIFDANNPDIFFDTAIQSALIAS
                     CCFVYLVPNPDGLPKMQVIEASKATGVIDTTTFLLTEGYAILEVDENDNPVLEAYFTK
                     DVTWYYPKGANPYSISNPTGQPLLVPIIHRPDAVRPFGRSRITRAGMYNQLAAKRTLE
                     RAEVTAEFYSFPQKYVLGTSQDADPMEKWKTTVSSLLEITKDDDGDSPNVGQFNTASM
                     APFIDQLRMYASLFAGGSGLTMDDLGFPSDNPSSVEAIKAAHENLRAAGRKAQRSIAS
                     GLLNVAYVAVCLRDRYQYTREQFLNTKIKWEPLFEADANMLTMIGDGAIKLNQALPGY
                     INAETIRDLTGIAGDMSATPKLAEVVANE"
     gene            complement(2732..3091)
                     /locus_tag="U1099_001778"
     CDS             complement(2732..3091)
                     /locus_tag="U1099_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868068.1"
                     /translation="MKKSCAFCGRSFTAESKRVKYCSDKCRKDGARDKQRKLMKQKRA
                     NLKKQKSKKDNPNNQPAKKRKKKKNLLKYYQDFKTKILANEAEFGFTSRTIVEGVEVH
                     EPDFEEQVINKIKEQPK"
     gene            complement(3183..3530)
                     /locus_tag="U1099_001779"
     CDS             complement(3183..3530)
                     /locus_tag="U1099_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM4868069.1"
                     /translation="MRADKSGTHRVAFEKNKKRILKTATHCGICGQLVDKKLKYPNPM
                     CAVIDHVVPLAKGGHPSLIENLQLAHMSCNRIKSDKLFADNSKAEQKTLGNRNLPQSR
                     DWSQFISVKSEGG"
     gene            complement(3627..4061)
                     /locus_tag="U1099_001780"
     CDS             complement(3627..4061)
                     /locus_tag="U1099_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEM4868070.1"
                     /translation="MNKAKAILKDLRNLDLYIASLIRRREKIEASLLSSPKWTADKVS
                     GGAKKKQDDVYVELMATADDIEAKTVEAIKKQRELQSMIDGLSNSDSQTVLSMVYIDK
                     MSPWQVMDALNCSESTYYRILRVATRELNEMTVNDRDLQKIQ"
     gene            complement(4134..4448)
                     /locus_tag="U1099_001781"
     CDS             complement(4134..4448)
                     /locus_tag="U1099_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868071.1"
                     /translation="MTLFDEVQQLSSESYDKWFERYFKKYDLEKVIKKSAMQGYSGHL
                     ISVLKVKDDYTRRRLDDERTLEKIKELLGDGFKVEYHSTYSKNLFTGEDFITNKQIHI
                     SW"
     gene            complement(4637..4852)
                     /locus_tag="U1099_001782"
     CDS             complement(4637..4852)
                     /locus_tag="U1099_001782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868072.1"
                     /translation="MTKTQEPCLAKIGKYWERADFLGVFQHSGTNLIFGHQFAGPVAV
                     VRFRGRLVKVEIENIDFCEVENEQAAT"
     gene            complement(4854..5162)
                     /locus_tag="U1099_001783"
     CDS             complement(4854..5162)
                     /locus_tag="U1099_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEM4868073.1"
                     /translation="MKEIDPLVLVTLCIVIALFAAVTEVKVLREQVKRLEEREMVIIH
                     KVDNAGVTMVGKVTRKDIIDGRYYVEIGAYGKFLVTKGQFETINIGDDIPDYLQGRGS
                     "
BASE COUNT         1444 a         1218 c          865 g         1788 t
ORIGIN      
        1 actcgatatc tcgaatgctt atccccaccc aaacgacaac tctttacgat aggtcccgtc
       61 ttggttttta aaattttgta aaaatttccc taaatacttc atgctcgcat cgtcgaatgt
      121 atttacacga atatcttttg tttcaggatt aaaaacaagc gtttgcttat gctcctcctt
      181 attttcaggg taaatctcat agtgataaaa accatcgtca ctaatatcta tcaaaacaaa
      241 tcttaaatac atacctttac cctttctttt taaattcgtc gacagcttcg gagtaattaa
      301 ataattcatc aacttttttg tgagctgtat cataatccaa cttgctactc acatacatat
      361 aatcatgttc taagcgttca tgcttcagca aaattaaatc atgtggttga atgttttttc
      421 cagaaatcaa tctatcccag gattgagcca tgtcaatgtc tggtgcaaac aaccccttcc
      481 cataattcaa ctcatgaaca ttattaaaca catgatcgaa cgctttatca acatcatctt
      541 ttgaaaagcc attcatttct ttgaacttcc aaatgttcga atagatttta cgtttttcca
      601 aatcttgatt gctgtttttt atcctgtcgt aggttaaata agcatgtttt tcggctttaa
      661 taaattcctt tggcaaaagc tcgtcgtttg aatcatcacg aacataattc tttgcgccag
      721 cttgaacaag tagtccgatg cctttccttt cctctatttt agcatttttt aaaggatcag
      781 accaacgttt tgagtgtgca ttctgttttt tgccgtttcc tgggtgataa tcaacagtac
      841 acctgcaccg attatgcctc ctccaaacat cctttggaac atctggatag ttataaaccc
      901 caacaacagc cctacaccat ttacaacaat taccactttc ttttctgaca actttaggtg
      961 tcaaccctgc cttggcatga aaatccgcat tcgttttgat actatcatcc acaattgact
     1021 gggtaaaatt gacaatcggt tctttcagca accacgacac tttttcaaaa tcaacttcag
     1081 atgacaagcg attgaccaag ccatcaactc tatcttgatt gaatttcgga acttggactt
     1141 ttagaccgat attagcttcc ttgttcaagt tcgcttggac ttgtcctgta aagtcgctca
     1201 caagctcaaa attttttcct agcacgtcat tcagtaaacg ttggccgata ttgtaataca
     1261 tttttccatc tggcaaccta tcgcccgcaa cagatgagcc aagagcttct gctaagatgt
     1321 ctccaagctc aatggcaaaa tcatttgctg tggaataggt tgcctttttg tttttgagct
     1381 tggcaaaagc agagctgata atgtcacttt ttccatagga agcttcaaac ttatcctgaa
     1441 cttctttcag taagctaggt aagatatcat cattcatttg ctaccacctc cgctaacttc
     1501 ggagtggccg acatatcacc agcaatacca gtcaaatccc gaatggtttc ggcgttgata
     1561 tagccaggta aggcttgatt aagtttgata gctccatcac caatcatggt cagcatgttg
     1621 gcgtcagctt caaagagcgg ttcccacttg atttttgtat tgagaaactg ctctctggtg
     1681 tattggtatc tatcccgtaa acaaacggcc acataggcta catttaacag accagatgcg
     1741 attgagcgtt gggcttttcg ccctgcagcc ctcaaattct cgtgcgccgc cttgatagct
     1801 tcgacagatg atggattatc cgatggaaaa ccaaggtcat ccatggtcag accgcttcca
     1861 ccggcaaata atgatgcata cattcgcaac tgatcaataa atggtgccat gctagcagtg
     1921 ttaaactgac cgacattagg actatctccg tcgtcgtcct ttgtaatttc aaggagactc
     1981 gacactgtcg ttttccactt ctccatcggg tcagcatctt gactagttcc cagaacatac
     2041 ttttgcggga acgagtaaaa ttctgctgtc acttctgcac gctctagggt tcgcttagct
     2101 gccaactgat tgtacatgcc tgccctagta attcgactgc gaccaaaggg acggaccgca
     2161 tcaggtctgt gaataattgg cactagtaaa ggttgaccgg tcggattgga gatactgtat
     2221 ggatttgcac ctttagggta ataccatgtc acatctttgg taaaatatgc ctctaatacc
     2281 gggttgtcgt tttcgtccac ttctaggatt gcatatccct cagtcagcaa aaatgttgtg
     2341 gtgtcaatga caccggttgc cttgctggct tcaatgacct gcattttagg caagccatca
     2401 ggatttggga ccaagtacac aaaacaacaa gatgcaatca aggctgattg aatagccgtg
     2461 tcaaaaaaga tatctgggtt gttagcatca aagatttcgg ttgcttcaaa atcatcattt
     2521 gcaaactcac ggaaaatgat tctgtcagcc aaggcatcca cactgtgagc cgtccatccc
     2581 ataaccgagt tatacatgct tttgacattg tcagggatga taaaactttt ggtattatct
     2641 ctgtctgcca tagcatagaa cctataacgt ttcttaactc ctaattgata tagagctaac
     2701 ttcttccgga gataactcat tcctttgtaa ctcattttgg ctgctcctta attttattga
     2761 tcacttgctc ttcaaagtct ggttcgtgga cttccacacc ttcgacaatg gtccgactag
     2821 taaaaccaaa ctcagcttca ttggccaaaa tcttggtttt aaaatcttga tagtatttca
     2881 gcaaattttt ctttttttta cgttttttgg ctggttggtt gttaggatta tcttttttag
     2941 atttttgctt tttgaggtta gcgcgttttt gtttcatcaa tttacgctgt ttgtctctag
     3001 ccccgtcctt acggcattta tcactgcaat atttaacccg ttttgattct gccgtgaagc
     3061 ttcgtccaca aaaagcacat gattttttta tcatttttga taactcctat accacttaat
     3121 cctaacgcga aaaaaaatgt acagtgacgg cgtgaagggc cgccgagcga gttggtgggg
     3181 ttctaccccc cttcagattt tacagagata aactgtgacc aatcgcgact ctgaggaaga
     3241 tttctatttc ccaatgtttt ttgttctgct ttagaattat ctgcgaataa cttatcagat
     3301 ttaattctat tgcaagacat gtgagccaac tgcagatttt caattaacga tggatgaccg
     3361 ccttttgcta atggaaccac atgatcgatt actgcacaca ttggatttgg atattttaat
     3421 ttcttatcaa ctaactgacc acatattcca caatgtgttg cagtcttaag tattctcttc
     3481 ttattctttt caaaagctac tctatgcgtt ccgctcttgt ctgctctcat cgttacctca
     3541 aacaaaagag gcaccgtata attacagcac ctctcaattt cttgatgata taataatagc
     3601 acgttgaaac tgtcatgcac tgtcaattac tgtatttttt gcaaatctct gtcatttact
     3661 gtcatttcgt taagttctct tgttgctaca cgtaggatac ggtaataggt ggactcactg
     3721 caattaaggg catccattac ttgccagggg ctcattttat ctatatagac cattgagagt
     3781 accgtttgag agtctgaatt actaagacca tcaatcatac tctgtagttc gcgctgtttt
     3841 ttaattgctt cgacagtctt tgcttcaata tcatctgctg ttgccatcaa ttcaacgtag
     3901 acatcatcct gcttcttttt cgctccgccc gaaactttat ccgctgtcca ctttggacta
     3961 gatagtaagg aggcttcgat tttttcacga cgtctaatca agctagcgat gtacaaatct
     4021 aaatttctca aatcctttaa tatagcctta gctttgttca ctctctttct cctttgtggt
     4081 ataatattga tagcgaaacc acagcctggg cagagagtgc cttggctttt ttgttaccat
     4141 gaaatatgaa tttgcttgtt agtgataaaa tcttcaccag taaacaaatt tttactatag
     4201 gtcgaatgat actctacttt gaaaccatct cctaacaatt ccttgatttt ctcaagggtc
     4261 ctttcatcat ccaatcttcg tctcgtataa tcatctttaa cttttaagac actaatcaga
     4321 tggccactat acccttgcat agctgatttc ttaatgactt tttccaaatc atatttttta
     4381 aagtaacgtt caaaccactt gtcatagctt tctgaactta attgctgtac ttcatcaaat
     4441 agtgtcattt caatcccatc ctttcattat tcagttttat aatcttgaaa taccatagta
     4501 ttcatagcca caatattcag agcaaaaacc gtatgtatta aaatatttgt caaatactcc
     4561 gattttttct ttacaaacag ggcaatgcgt tctacggtat ctttcttctg tactcaatcc
     4621 attcagtatt ttctttttac gttgccgctt gttcattttc cacctcacaa aaatcaatat
     4681 tctcaatctc tacttttacc agtctacctc taaatctaac tacagcaact ggtccagcaa
     4741 attgatgacc aaagataaga tttgtcccag aatgttgaaa cactcccaaa aaatcggctc
     4801 tttcccagta tttacctatt tttgccaagc aaggctcttg agtttttgtc atctcaacta
     4861 ccccttcctt gtaggtaatc cggaatatca tcaccaatat taatcgtttc aaactgaccc
     4921 tttgtgacta ggaatttgcc gtaggcaccg atttctacat agtacctgcc gtcaataata
     4981 tccttccttg tgacttttcc gaccatagtc acgcccgcat tatcgacttt atggataata
     5041 accatttcac gttcttccag tcttttcact tgttcacgca atacttttac ttctgtcact
     5101 gctgcaaata aagcgatgac aatacacaga gtcaccaaaa ccaatggatc aatttctttc
     5161 atttaacttc ctccatcttc acactgtaca accgctgacc tcgataccta gcttccagcc
     5221 ctgctttaca tttcaaagca tcagcttcat tttcaaagta atgcgtttca tcaactaaca
     5281 tgtgatcaaa taatactgtt actgtatatg tcatt
//
LOCUS       DASFSW010000132         5205 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.133, whole genome shotgun sequence.
ACCESSION   DASFSW010000132 DASFSW010000000
VERSION     DASFSW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.133"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            75..824
                     /locus_tag="U1099_001784"
     CDS             75..824
                     /locus_tag="U1099_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEM4868074.1"
                     /translation="MQKIDLLDGERIDQLFSTDVQIIQNREVFSYSIDSVLLSRFPKM
                     PAQKGLIVDLCSGNGAVGLFASTRTKAQIMQVELQERLADMNRRSIALNGLEEQVSVI
                     NDDLANLPQYDLRSKVDLMLCNPPYFKVDKDSNLNESEHYLLARHEIATNLDSICQVA
                     QQVLKSNGRLAMVHRPDRFLDILDKLRTYKLAPKRIQFVYPKASKEANMLLIEAIKDG
                     SVDGLHILPPLVVHEENGDYTPAIREIYYGK"
     gene            814..1086
                     /locus_tag="U1099_001785"
     CDS             814..1086
                     /locus_tag="U1099_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM4868075.1"
                     /translation="MENKAYFYVLECADGSLYTGYTTDVEKRLATHNRGKGSKYTRGR
                     LPVSLLYQEAFASKQEAMSAEALFKKRSRQSKLDYIAEMTKKPRTK"
     gene            complement(1102..1713)
                     /locus_tag="U1099_001786"
     CDS             complement(1102..1713)
                     /locus_tag="U1099_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM4868076.1"
                     /translation="MVTVLIVKAHPLDAQKSYALRALEEFQTRYASLHPEDQIEVVDV
                     FEDQIPALDKSLLEAMGAAKKGESISPDQAKQLDRYNALTQQFLMADKIVVVNPLWNL
                     NVPSQLVSWVNTINVAGLTFKYGPEGSIGLVKDKKLLHIQSNGGVYAGQDPAAQYIKS
                     IFEFLGFEDIHQVFIEGQSADPSQAQAIFEEAMGKIDAILEVF"
     gene            complement(1836..2243)
                     /locus_tag="U1099_001787"
     CDS             complement(1836..2243)
                     /locus_tag="U1099_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4868077.1"
                     /translation="MITYKQNPQLDFQAVLEIYDSVGWTNYTDRPTMLQKALEHSLLV
                     LAAFDGDRLVGLLRAVGDGHSIVFIQDILVLPTYQRQGIARQLLEQAIAYFPGIYQLH
                     LLTDNTEKTRSFYEAIGFTAVDSLDSVAYTYLK"
     gene            complement(2355..2957)
                     /locus_tag="U1099_001788"
     CDS             complement(2355..2957)
                     /locus_tag="U1099_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HEM4868078.1"
                     /translation="MKKIDIKTLFATSSIFLLIALLTIYYYGALPDTMVTHFGVNGEP
                     NGSMPKYMVTILTPLLFFCVHLFICILYDVKGIGKTPVIRVFKWLFPLLALIIQVSLL
                     WYNLGGELDYRRLVIGLLAVYYMVIGNYLPKEELDSKTNEIERKGRKQSGYLLMIGGL
                     LQLVSLLGSPTLSILVMILVGISVVSLSIYYAYLHFKTAS"
     gene            complement(2944..3222)
                     /locus_tag="U1099_001789"
     CDS             complement(2944..3222)
                     /locus_tag="U1099_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM4868079.1"
                     /translation="MGLSETLKAISAPIRRQILDSLKSGPKSAGEIVEQFQLTGATVS
                     HHLSTLKKAGLILEEKQKNFIYYRLNYTVFEEVLVWIASFGGQQDEEN"
     gene            complement(3367..4947)
                     /locus_tag="U1099_001790"
     CDS             complement(3367..4947)
                     /locus_tag="U1099_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM4868080.1"
                     /translation="MKFTDLQLSEDILLAVEKAGFETPSPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIDTNNQAIQALIIAPTRELAVQSQEELFKFGREKGVKVRS
                     VYGGSSIEKQIKALRSGAHIVVGTPGRLLDLIKRKALKLDGVETLILDEADEMLNMGF
                     LDDIEAIIERVPESRQTLLFSATMPEPIKRIGVKFMKEPEHVKIAAKELTNVNVDQYY
                     LRVKEHEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     QSGQSITFVSPNEMGYLAIIEDLTKKRMKGLKPATAQEAFEAKKKVALKKIEREMADE
                     TIRSNFDKFKKDAIQLAAEFTPEELALYVLTLTVQDPESLPEVEIAREKPLPFKFAPG
                     QAKGKGGRGGRDRDRGGRRDGDRNRDRGGRRGDRNDRNRRDDKFKRDNRRQDNKKPHQ
                     RTSSEKKTGFVIRNKGER"
BASE COUNT         1447 a         1179 c         1051 g         1528 t
ORIGIN      
        1 ccagggcttt ttgcccagcc tcttttcttt caaaatatac agtgattttc attcaaagat
       61 ggtataatat acctatgcaa aaaattgatt tactagacgg agagcggatc gaccagctct
      121 tctcgacaga tgttcagatt attcaaaatc gagaggtttt tagctactcg attgacagcg
      181 ttcttctttc gcggtttcca aaaatgcctg ctcagaaggg gctgattgtt gacctttgta
      241 gcggaaatgg ggccgtgggt ctctttgcct ccactcgcac caaggctcag attatgcagg
      301 tcgagttgca ggaacgcttg gcggatatga accgccgctc cattgccctc aatggtttgg
      361 aggaacaggt ctcggttatc aatgatgact tggcaaatct gccccagtat gatctgcggt
      421 ccaaggttga tttgatgctc tgcaatcctc cctacttcaa ggtagataaa gactcaaacc
      481 tcaatgaaag cgagcattat ctcctagcta gacacgagat tgcgaccaac ctagacagca
      541 tttgtcaggt ggcccaacag gtgctcaagt ccaatggtcg cttggccatg gttcacaggc
      601 ccgaccggtt tttagatatt ttggacaagt tgagaaccta caaattggct cccaaacgca
      661 tccagtttgt ctatcccaag gccagcaagg aggccaatat gttgctgatt gaggctatca
      721 aggacggctc ggtggacggt ctgcatatcc tgccacctct ggttgtccat gaggaaaatg
      781 gcgactatac tccggctatt cgtgagattt attatggaaa ataaggccta cttttatgtt
      841 ttggaatgtg ccgacgggag tctttatact ggctatacga ctgatgtgga gaagcggctt
      901 gctacccata atcgtgggaa agggtccaag tacacccgcg gacgcttgcc tgtcagcttg
      961 ctctatcaag aagcctttgc tagtaagcag gaagccatgt ctgccgaggc tttgtttaag
     1021 aaacgcagtc ggcagagtaa attagattat attgcagaaa tgacaaaaaa acctcgtaca
     1081 aaatagtacg aggcttttta tttaaaatac ttccaaaatt gcatcaattt tccccatagc
     1141 ttcttcaaaa atagcctgag cctggcttgg atcggcagat tgcccttcga taaagacttg
     1201 atgaatatct tcaaagccta aaaattcaaa gatagacttg atgtactggg ctgctggatc
     1261 ctgacctgca taaacaccac cattggactg gatgtgcagg agctttttgt ccttgaccaa
     1321 accgatagag ccttctggac catacttaaa ggtcaggcct gctacattga tggtattgac
     1381 ccaagatacc aattgacttg gcacattgag attccagaga ggattgacga caactatctt
     1441 atccgccata agaaattgct gggtcaaggc attgtaacgg tccaattgct ttgcttgatc
     1501 aggactgata ctctcgcctt ttttagcagc tcccatagcc tctaacaaag atttgtccaa
     1561 ggctggaatc tggtcttcaa aaacatccac tacttctatc tgatcttctg ggtgcaaact
     1621 ggcgtaacga gtttgaaact cttccaaagc ccgcaaggcg taggactttt gtgcgtcgag
     1681 ggggtgggcc tttacaatta atacagttac cattgttcat ctcctttgtt actaattatt
     1741 gataattata ttctactctt tttgatttga aattcaaatc atttgctcga agcaaaaggc
     1801 tggtctccca gcctctgctt ttttagattg ttttattatt ttaaataggt gtaagcgaca
     1861 ctgtccaggc tatcaacggc tgtaaagcca attgcctcat agaaagaccg tgttttctca
     1921 gtattgtccg tcaagagatg gagttgataa atacctggaa agtaggcaat ggcctgctcc
     1981 aaaagctgac gcgcaattcc ttgtcgctga taggttggta ggaccaagat gtcctgaata
     2041 aaaacgattg aatgcccgtc gccaactgct cgcaagagac ccactagacg gtcaccgtca
     2101 aaggcagcaa gaacgagcag actatgttcc aaggccttct gtaacatggt aggccggtca
     2161 gtataatttg tccaaccaac tgaatcatag atttcaagga ctgcttgaaa gtcgagttga
     2221 ggattttgtt tgtaagtaat catgtctttc tcctttgtat tgttttttag aatacaaagg
     2281 ctttgtccat tgttaaaacg cccatggtct tacctcctgt tttactacat ttagtataac
     2341 aaaaactgct ccaattatga agcggtttta aaatgcagat aagcataata gatactaaga
     2401 cttacaacag aaataccaac caatatcatt acaaggatag atagggttgg tgaacccaac
     2461 aaacttacta actgcaacaa cccaccaatc atcaagagat agcctgactg ctttcttccc
     2521 tttcgttcta tttcattggt cttgctatcc aattcctcct tgggtaaata atttcctatc
     2581 accatataat agacagccag cagtccaatg acaagcctac ggtaatccaa ttctcctcct
     2641 aaattatacc agagtaaaga aacttgaatg atgagagcca gtagcggaaa aagccatttg
     2701 aacactcgaa tgaccggtgt tttccctatc cctttcacat cataaagtat acaaatgaat
     2761 aggtgaacac aaaagaatag tagcggtgtc aaaattgtca ccatatactt gggcatagag
     2821 ccatttggct caccatttac gccaaaatgc gttaccatgg tatctggcaa tgctccatag
     2881 taataaatcg tcaataaagc aatcagtagg aatatactag aagttgcaaa taaggttttt
     2941 atgtcaattt tcttcatctt gttgccctcc aaaacttgca atccaaacca agacttcttc
     3001 aaagaccgta tagtttagac gataataaat aaaatttttc tgcttttcct ccaaaattaa
     3061 tccagctttt ttcaaagtgg ataaatgatg agaaactgtt gctcctgtta attgaaattg
     3121 ttctacaatt tccccagcag acttcggccc cgacttaaga ctatctagaa tttgcctacg
     3181 aataggagca gaaattgcct tcaaggtttc agacaatccc ataataagcc tccttgtcca
     3241 tctatttaga aatatttcta aatagaatta tactctgttt ttattgtttg tcaagaacta
     3301 tttcgattat tttctaaata gatttcaaaa aaaacagacc actctttatg gtctgtcaaa
     3361 tccggcttat ctctcccctt tattgcggat aacaaagcct gttttctttt cacttgatgt
     3421 gcgttggtgt ggttttttgt tgtcctgacg gcggttgtcg cgtttaaact tgtcatcgcg
     3481 acggttgcgg tcattacgat caccacggcg tcctccgcgg tctcggttgc ggtctccatc
     3541 acgacgacca ccgcgatcac ggtcgcgacc accacgtcca cccttgccct tggcttgacc
     3601 aggtgcaaac ttgaatggca gtggtttttc tcgcgcaatt tctacctctg gcagactttc
     3661 tggatcctga acagtcaagg tcaatacata aagcgctaac tcttcagggg taaattcagc
     3721 cgctaactga atagcatctt tcttgaactt atcaaaattg ctacggatgg tttcatccgc
     3781 catttcacgc tcaatcttct tcaaagcaac tttcttcttg gcttcaaatg cttcctgagc
     3841 agttgctggc ttgagacctt tcatgcgttt cttggtcaaa tcttcgatga tggccaagta
     3901 gcccatctcg tttggcgaaa caaaggtaat agactgacct gattgacccg cacgacctgt
     3961 acgaccgata cggtgcacat agctttcagg gtcctgtgga atatcgtagt tatagacatg
     4021 cgttacacct gagatgtcca aaccacgggc cgcaacgtct gtcgcaacta agacatcaat
     4081 ttgatcattt ttaaaatcac gaatgacacg aagacgcttg ttctggtcca aatctccgtg
     4141 aataccttct gcacggaaac cacgcaattt caaaccacga gtcaattcat cgacacggcg
     4201 tttggtacga ccaaagacaa tagacaattc tggctggtca acatccataa gacgagtcat
     4261 ggtgtcgaat ttttcatgtt ctttgacacg aagataatat tggtcaacat tgacattagt
     4321 caattccttt gctgcaatct taacgtgctc aggttctttc atgaacttga caccaatacg
     4381 cttgattggc tctggcatgg ttgcagaaaa cagaagggtt tgacggctct ctggtacacg
     4441 ttcgatgatc gcttcaatat catccaagaa gcccatgttg agcatttcat ctgcctcgtc
     4501 aagaatgaga gtttcaacac cgtcaagttt gagggccttg cgtttgatca agtccaagag
     4561 gcgacctggt gtaccaacaa cgatgtgagc acctgaacga agggccttga tttgcttttc
     4621 aatgcttgag ccaccgtaaa cagagcggac tttcacaccc ttttcacgac caaatttgaa
     4681 aagttcttct tgactctgca ccgccaactc acgtgttggg gcgataatca aggcctgaat
     4741 tgcttgattg tttgtgtcaa ttttgttgag ggttggcaaa ccaaaggctg cagttttccc
     4801 tgtccccgtc tgggcttgac caatcacatc tttaccttca agagcaagcg gaatggtctg
     4861 ctcctggatt ggtgacggcg tttcaaaacc agccttctca acggcaagca aaatatcttc
     4921 tgataattgt aaatctgtaa atttcaatgt attctctttt ctaaaaggca gtgcgaagct
     4981 accttatcta acttttttgt aacaagatat cagggtcgaa ccactgatat tggatttgca
     5041 acttaccaag tataacacaa tctaacaata atagttaata tttaccttta aaaagtcaat
     5101 aatacgtttt catgaggagt ccatcatttt tatagacatt tgaattaact ttgatacatc
     5161 gatactttcg gtttgcccta ctctagtacc agctccttgt ctgaa
//
LOCUS       DASFSW010000133         5178 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.134, whole genome shotgun sequence.
ACCESSION   DASFSW010000133 DASFSW010000000
VERSION     DASFSW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.134"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            84..449
                     /locus_tag="U1099_001791"
     CDS             84..449
                     /locus_tag="U1099_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM4868081.1"
                     /translation="MTVKINTKDGQIELTDDVIATVVGGATTEIFGVVGMASKSAIKD
                     NFQALLRKENYSKGVVIKTLEDGRIAVDVYTVMSYGVKISEVSRNIQERVKFNLENQL
                     GISVDAVNVFIQNIKVVGE"
     gene            452..2116
                     /locus_tag="U1099_001792"
     CDS             452..2116
                     /locus_tag="U1099_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEM4868082.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTITNGAKEVADKSASTVGEVAQILSKGLLMGARGNSGVITSQLFRGFGQSVKGKVE
                     LDGKDLAQAFQHGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     DGANRALKKTPEMLPVLKEVGVVDSGGQGLVYIYEGFLSALTGEYIASEDFEATPAVM
                     SEMINAEHHKSVAGHVATEDITFGYCTEIMVALRQGPTYVKDFDYEEFRNYLNDLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQAGLQYGSLVKVKVDNMREQHEAQVEKVESFQKPA
                     EQKEYAIIAVAAGEGLTEIFKSQGVDYVISGGQTMNPSTEDFVNAIELVNARNVILLP
                     NNKNILMAAQTAAEVAEVAVKVVETKTLPQGFTSLLAFDPSRDLESNFEAMTASLAEV
                     KSGSVTTAVRDTTIDGLEIHENDNLGMVDGKIVVSNPDMMETLVATFDKMLDEDSEIV
                     TIYIGEDGDEELAQTLAEQLEEQFEDVEVEIHQGNQPVYPYLFSVE"
     gene            2181..2657
                     /gene="tsaE"
                     /locus_tag="U1099_001793"
     CDS             2181..2657
                     /gene="tsaE"
                     /locus_tag="U1099_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEM4868083.1"
                     /translation="MTNYWGYGIIKDMYSQNENELIAIGERIGKACKPNQVLVLSGDL
                     GAGKTTLTKGLAKGLKIEQMIKSPTYTIVREYEGTMPLYHLDVYRIGDDPDSIDLDDF
                     LYGGGLTVIEWGELLDVSLFDDYLLIRIEKEGDGRRLTVESHGAQSSDLAKEFQNV"
     gene            2650..3177
                     /locus_tag="U1099_001794"
     CDS             2650..3177
                     /locus_tag="U1099_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4868084.1"
                     /translation="MSEKEVYFSEAEPADAAAFIDFMNQVARETDYLVMDETGFRFSV
                     DEMETIFEAGIENPRELCLLAKVGSEVVGAISVKSSKQFRISHIGNIFIAVKKAYWGH
                     GLGTILLEEVLHWAEEMDVLKRLELTVQVRNQAAVHLYQKLGFNIEGTQIRGARTDEG
                     EWLDLYYMGKLIGEV"
     gene            3174..4367
                     /locus_tag="U1099_001795"
     CDS             3174..4367
                     /locus_tag="U1099_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM4868085.1"
                     /translation="MTIGKKIFLMSLAIIGLTVGAGLIYGASLLNFSTDAISKTFKQL
                     DGEEKITPIDATEPLTILLMGVDMDQATRGGDWEGGRSDSMILVTVNPKTKETNMMSL
                     TRDIMVEIAEANGESSGTVEKLNHSYSYGQAPMAIATIEKMMDINIDRYIEINMDGLV
                     ELVDAVGGIEVNNTLGFPISISEHEPAYTSIVQPGKQLVNGDQALVYARMRYDDPEGD
                     IGRQRRQREVITAIIKKLLQLDGFTQYKKILTAISNNMRTNIEISPATIPNLLGYKDS
                     ISKLNSYQLRGVDQMVDAIYYQLPTSTHLLEMQNVLKKSIGLEENTDLVTNVKVYEGQ
                     MGLPSPINVYDVYTNLLLLEASPWAESLDPEIGSPSATTTVTTEEQVTDFSTEPVLGD
                     ENGSQ"
     gene            complement(4476..4778)
                     /locus_tag="U1099_001796"
     CDS             complement(4476..4778)
                     /locus_tag="U1099_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEM4868086.1"
                     /translation="MKLRNIILAVSAATASFLAVTHRDKIAKEVQETKQLLSDIQLNK
                     DNIQEQLAIIQSFQEPLQEMASDLQYKTRVYQQSIAGNLEEIQQILKKYQNENNTP"
     gene            complement(4780..>5178)
                     /locus_tag="U1099_001797"
     CDS             complement(4780..>5178)
                     /locus_tag="U1099_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEM4868087.1"
                     /translation="FCKIISGEIPASKVYEDDQVLAFLDITQATKGHTLVVPKKHYRN
                     VLDMDKEAAATLFSVVPTIARQLKEKLGASGLNIVNNNEEAAGQTVFHTHIHLLPRFN
                     NQDGLSIQFKANDPDFPALAQLAQELYLGE"
BASE COUNT         1504 a          876 c         1278 g         1520 t
ORIGIN      
        1 aatccgatgt aacaagcttg ttttttacag aaagcagaaa atgtggtaaa atggagtgat
       61 aaaactattt gaggtaacga attatgactg ttaaaatcaa tactaaagac ggccaaatcg
      121 aattgactga tgacgtgatt gccacagttg ttggcggtgc tacgactgag attttcggtg
      181 ttgttgggat ggctagtaag tctgcgatca aggataattt tcaagcactt ttgagaaaag
      241 aaaattattc taaaggtgtt gtgattaaga ccttggaaga tggtcgcatc gcagtcgatg
      301 tttatactgt gatgagctac ggtgtaaaaa tcagtgaagt atctcgcaat atccaagaac
      361 gcgttaagtt caacttggaa aatcaactag gtatctctgt agatgccgtc aatgtcttta
      421 tccaaaatat caaagtcgta ggagaataat tgtgtctaaa attacaacta gtttatttca
      481 agaaatggtg caggcagcat caactcgttt gaataaacaa gccgaatatg tgaactcact
      541 caatgttttc ccagttccag atggggatac tgggaccaac atggggatga ctattaccaa
      601 tggtgctaaa gaagtagcag ataagtctgc ttctactgtt ggagaagttg cgcaaattct
      661 ttctaagggc ttgttgatgg gagctcgtgg aaactcaggt gttattacgt cgcaattgtt
      721 ccgcggtttt gggcaatctg ttaaaggcaa ggttgaattg gacggtaagg atttggcgca
      781 agcctttcaa catggtgtag aagtggctta taaagccgtt atgaagccag ttgaaggtac
      841 aattcttact gtttcgcgtg gggcagccac tgccgctttg aaaaaggcag aagagactga
      901 tgatgcggtt gaggtcatgc gtgccactct tgacggtgct aatcgtgctc ttaagaaaac
      961 gccagagatg cttccagtat tgaaggaagt tggcgttgtc gattcaggtg gacaaggtct
     1021 tgtttacatt tatgaaggct ttttgtcagc tttgacgggt gagtatattg cttcggaaga
     1081 ttttgaggcg acacctgctg ttatgtctga aatgatcaat gctgagcacc acaagtcagt
     1141 tgcaggtcat gtagcgactg aagatattac ctttggttac tgtacagaaa tcatggtagc
     1201 tcttcgccag gggcctactt atgtcaagga ctttgattat gaagaattcc gtaactacct
     1261 taacgacctt ggggattccc tccttgtggt caacgatgat gagatcgtta aagtccatgt
     1321 ccatacagaa gatccaggtt tggtcatgca agcgggtctt caatatggta gcttggtcaa
     1381 ggtaaaagtg gacaatatgc gtgagcaaca tgaagctcag gttgagaaag tagaaagttt
     1441 ccaaaaaccg gctgaacaaa aagagtatgc tattattgcg gtagctgctg gggaaggttt
     1501 gacagaaatc ttcaaatcac aaggcgttga ctatgtgatt tcaggtggtc agaccatgaa
     1561 cccatcaaca gaagattttg tcaatgctat tgaattggtc aatgcccgca atgtcatctt
     1621 gttgccaaac aacaaaaata tcttgatggc tgctcagacg gctgctgagg ttgcagaagt
     1681 tgcggttaag gttgtggaaa ctaagaccct accacaaggt tttactagct tgcttgcttt
     1741 tgatccaagt cgtgatttgg aaagcaactt tgaagcgatg acggcatctc tcgcagaggt
     1801 gaaaagtggt agcgtgacaa ctgcggtccg cgatacaacc attgacggtc ttgaaatcca
     1861 tgagaatgac aaccttggta tggttgatgg caagattgtg gtttctaatc cagatatgat
     1921 ggagacactt gtggctacct ttgataaaat gttggatgaa gacagcgaaa tcgtgactat
     1981 ctacatcggt gaagatggtg atgaggaact cgctcaaact cttgctgaac agctagaaga
     2041 acaatttgaa gatgttgagg tcgaaatcca tcaaggaaat caaccagtct atccatatct
     2101 tttcagcgta gaataaacaa aatcgcaggc tctggcttgc gattttttct gtcgacttgt
     2161 ctacaatttc cagtagacct atgacaaact actggggcta tggtataatt aaagacatgt
     2221 atagtcagaa tgaaaatgaa ttgattgcca ttggtgagag aattggaaaa gcctgtaagc
     2281 caaatcaagt tctagtatta tcaggggatt tgggtgctgg gaaaacaact ctgaccaagg
     2341 gtttggccaa gggtttaaaa attgaacaga tgattaagag tcctacttat acgattgtgc
     2401 gagagtatga ggggaccatg ccactctatc acttagatgt ttatcgaatt ggagatgacc
     2461 ctgactcgat tgatttggat gattttctct atggaggagg tctaacggtt atcgagtggg
     2521 gagaattact ggatgtcagt ctatttgatg actatttgct cattcgtata gagaaagagg
     2581 gagatggtcg acgattgaca gtcgaatctc atggagctca aagtagcgat ttagcgaagg
     2641 agttccaaaa tgtctgaaaa agaagtgtat tttagtgaag ctgaaccagc tgatgcagca
     2701 gcttttattg attttatgaa tcaagttgct agagaaacgg attatctggt catggatgag
     2761 actggttttc gatttagtgt tgatgaaatg gagaccatct tcgaagcagg aatcgaaaat
     2821 cctcgtgaat tgtgcctgtt ggctaaggtt ggttcggaag tcgtcggcgc aatttctgtt
     2881 aagtcctcta agcagtttcg cattagccat atcggaaata tttttattgc agtaaaaaaa
     2941 gcctattggg gtcacggtct agggacgatt ttactggagg aagtattgca ttgggcagag
     3001 gaaatggatg tattgaagcg tttggaatta acggttcagg ttcgcaatca ggcggcagta
     3061 catttatatc aaaaactagg atttaacatt gaaggaaccc agatacgtgg ggctagaaca
     3121 gatgaaggag aatggcttga tctttattat atgggcaagt taattggtga agtatgacaa
     3181 ttggaaaaaa aatattctta atgtctttgg ctattattgg tttaacagta ggtgctggct
     3241 taatatatgg agccagcttg ttaaattttt ctacggatgc tatttcgaaa acatttaagc
     3301 agttagatgg agaagaaaaa atcactccga ttgatgcgac agagccccta actatcctac
     3361 tgatgggggt ggatatggac caagcaaccc gtggaggaga ttgggagggt ggtcgtagtg
     3421 attcgatgat tttggtaact gttaatccca agactaagga aaccaatatg atgagcttga
     3481 ctcgtgatat tatggttgag attgcagaag cgaatgggga gtcttcagga acggttgaaa
     3541 aactaaatca ttcttatagc tacggacagg caccgatggc cattgccacg atcgaaaaga
     3601 tgatggatat taacattgac cgctatatcg aaataaatat ggatggtctg gttgagcttg
     3661 ttgatgcggt tggaggtatt gaggtaaaca atactcttgg cttccctata tccatttcgg
     3721 aacatgagcc tgcctacacc tctattgtcc agccggggaa acagttggta aatggggatc
     3781 aggcgctggt ttatgctcgg atgcgctatg atgatcctga gggggatatt ggtcgtcaga
     3841 gacgtcaacg tgaagttata acggctatta taaaaaaact ccttcagtta gacggtttta
     3901 cccagtataa gaagattctg actgccattt ctaataatat gcgaaccaac attgagatta
     3961 gtcctgcgac aattccaaat ctcttggggt acaaggactc tatctccaag ttgaattcct
     4021 atcagttgcg cggtgtggat caaatggttg atgcaatcta ttatcaatta ccaaccagta
     4081 cacatctatt ggaaatgcaa aatgttctga aaaaatctat cggcttagaa gaaaataccg
     4141 acttagtcac aaacgtgaag gtctacgaag gacagatggg attgccaagt ccaataaatg
     4201 tttatgatgt atatacaaat ctcttgctgt tagaagcaag tccatgggca gaaagcttgg
     4261 atccagaaat agggagtcca tcggctacta cgactgtgac aacagaagag caggtaaccg
     4321 atttttcaac agagccagta ctcggtgatg aaaacgggtc acaataatgt agtcattcag
     4381 tttttctttt attactttga cgagcgagta taaaagccta taaaaataga ggttgtttgc
     4441 aacctctatt tttatatatt tggaaattcc ctatgtcagg gagtgttatt ctcattctgg
     4501 tattttttga gaatctgttg gatttcttcc aagtttcctg caatactttg ctgatagact
     4561 cgagtcttat attgtaagtc agaagccatt tcctgcaagg gttcttgaaa actttggata
     4621 atggcaagtt gttcctgaat attgtccttg ttcagttgaa tatctgatag aagttgtttg
     4681 gtttcctgta cttctttagc gattttatcc cgatgcgtga ctgcaaggaa gctggcggta
     4741 gcggcgctta cggctaaaat gatattacga agtttcatat tattctccta gatagagctc
     4801 ttgtgccagt tgagccaggg ctgggaagtc agggtcgttg gctttaaatt gaatgctcaa
     4861 tccgtcttga ttgttaaagc gtggtaagag atggatgtgt gtatgaaaga cggtttgccc
     4921 agcggcctcc tcgttattat taacgatgtt tagaccacta gctccaagtt tttcttttaa
     4981 ctgacgagcg atggttggca caacagaaaa gagtgtggct gcagcttctt tatccatgtc
     5041 aagcacgttt cggtagtgtt ttttagggac aaccaatgtg tgtccttttg tggcctgagt
     5101 gatgtctaaa aaggctaata cttggtcatc ttcatagact tttgaagctg gtatttctcc
     5161 agagataatt ttgcaaaa
//
LOCUS       DASFSW010000134         4965 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.135, whole genome shotgun sequence.
ACCESSION   DASFSW010000134 DASFSW010000000
VERSION     DASFSW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.135"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(162..1646)
                     /locus_tag="U1099_001798"
     CDS             complement(162..1646)
                     /locus_tag="U1099_001798"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEM4868088.1"
                     /translation="MTLVYQSTRDAKNTVSASQAILQGLATDGGLFTPISIPAVDLDF
                     SVLKDASYQEVAKLILSAFLDDFTADELDYCINNAYDSKFDTPAIAPVVKLKGQYNLE
                     LFRGSTIAFKDMALSILPYLMTTAAKKHGLENEIVILTATSGDTGKAAMAGFADVPGT
                     QIIVFYPRDGVSKVQELQMTTQTGANTHVVAIDGNFDDAQTNVKRMFNDEALRAKLAA
                     KKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIAAGDKVNFTVPTGNFGNILAAY
                     YAKQIGLPVGKLICASNDNNVLTDFFSTGVYDKNRTFRVTTSPSMDILVSSNLERLIF
                     HLFDNDAAKTAELMEALNTAGQYDIQGADADILSLFAAAFATEEETAAEIKRVYDESD
                     YIEDPHTAVASAVYKQYVEQTGDQTPTVIASTASPYKFPVVAVEAVTGQSGLSDFEAL
                     AKLHEISGVALPPAVDGLETAPVRHNTVVAAADMQAEVERYLGV"
     gene            complement(2061..4712)
                     /gene="adhE"
                     /locus_tag="U1099_001799"
                     /gene_synonym="adhC"
     CDS             complement(2061..4712)
                     /gene="adhE"
                     /locus_tag="U1099_001799"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEM4868089.1"
                     /translation="MADKIVVSPEEKLVEARKHVDELVQKGLVALDEFRKLGQEEVDY
                     IVAKASVAALDKHGELAMHAFEETKRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DEVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLIALKTRNPIVFAFHPSAQESSAHA
                     ARVVYEAAVAAGAPENCIQWITKPSMEATSELMKHDGIATILATGGNAMVRAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEVYDEFVTE
                     FKSYKTYFVNKKEKALLEEYCFGVKANSKNCAEGKLNADIVGRPAAWIAEQAGFSVPE
                     GTNILAAEVAEIGEKEPLTREKLSPVIAVLKVEGRAEGLEAARQMVEFHGLGHSAAIH
                     TEDAELAKEFGTIVRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSISDNVS
                     AMNLLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDRAMVELGF
                     LDRIIEQLDLRRNKVVYQIFSDVEPDPDITTVYKGTELMRTFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPTVDFHDLVQKFMDIRKRAFKFPELGKKSKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPAHVTADTGMDVLTHATEAYVSTVA
                     NDFTDGLALQAIKLVFENLESSVKNADFVSREKMHNASTMAGMAFANAFLGISHSMAH
                     KIGGRFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYKADEKYQDIARLLGLPC
                     STPEEAVAAYAQAVYDLGERIGIQMNLKAQGIDEKELKEHSRELALLAYEDQCTPANP
                     RLAMVDHMQEIIEDAYYGYKERPGRIK"
BASE COUNT         1360 a         1094 c         1043 g         1468 t
ORIGIN      
        1 ttctctggag attttctcct gttcttgtgc catacgccac ctcatctaca agtattccta
       61 tatagtatat cattctttga attatctgta aattttaatt taaacagcaa aaagaaaaca
      121 aactgttcaa aatgagcagt ttgtcataaa ataaatcgaa attaaacacc caaataacgc
      181 tctacttcag cctgcatatc ggctgccgca acaacagtat tgtggcggac aggtgccgtt
      241 tccaagccgt ccacagctgg tggcaaagct acacctgaaa tctcatgcaa ttttgcaagg
      301 gcttcaaagt cagacaaacc tgactggcct gtcacagcct caaccgcaac tactgggaac
      361 ttgtaaggac ttgctgtaga agcaatcaca gtcggagttt ggtcccctgt ttgctccaca
      421 tactgtttat agacagctga ggcaacagcc gtatgtgggt cttcaatgta gtctgactcg
      481 tcatagactc gcttgatttc cgcagccgtt tcttcttccg tcgcaaaggc ggcagcaaag
      541 agagagagga tatccgcgtc agccccttga atatcatact gaccagctgt gttcaaggct
      601 tccatcagct ccgctgtttt tgcagcgtca ttgtcaaaga gatggaaaat caagcgctcc
      661 aagttagaag acaccaagat gtccatggac ggactggtgg tcacgcggaa ggtacggttc
      721 ttgtcgtaaa cgccagtcga gaagaagtcg gttaggacat tgttgtcgtt ggaagcacag
      781 atgagcttgc caactggcag accgatttgt ttggcgtaat aagccgccaa gatgtttcca
      841 aagtttcctg ttggaacggt gaagttgacc ttgtcgcccg ccgcaatctc gccagtctta
      901 accagctgag cgtaggcata gacatagtaa acaatctgtg gcaccaagcg gccgatgttc
      961 atagaattgg cagatgaaaa ctgaagtttc ttcgctgcca acttggcacg gagagcctca
     1021 tcgttgaaca tacgtttgac atttgtctgg gcgtcgtcaa agttaccgtc aatggccacc
     1081 acgtgcgtat tagcccctgt ctgcgtagtc atctgcaatt cttggacctt agataccccg
     1141 tcacgtgggt agaagacaat gatttgcgtg ccaggaacgt ctgcaaaacc agccatagct
     1201 gccttacctg tatcgcctga agtcgcggtc aaaatgacaa tctcattttc caaaccatgc
     1261 tttttcgccg cagtcgtcat caagtaaggg aggatggaca gagccatatc cttaaaggca
     1321 atggttgaac ctcggaagag ttccaaattg tactgacctt ttagcttaac cactggggca
     1381 atagctggtg tgtcaaactt gctatcgtag gcattgttga tacagtagtc caactcatct
     1441 gccgtaaaat cgtccaagaa agccgacaaa atcaacttgg caacttcttg ataagaagcg
     1501 tctttgagaa cagaaaagtc caagtcaact gctggaatag aaatcggtgt aaacaaacca
     1561 ccgtcggtcg ccaagccctg caatatcgcc tgactagccg atacagtatt tttagcatcg
     1621 cgtgttgatt gatatactaa tgtcatggta tccctcataa aaaatctatt ttttctattc
     1681 taccacaaat tgacagaaaa ttgtgaaaga tagatagaaa agtctctttt tctcggctaa
     1741 accctaactt tccaaccact ttcgcttttt acaggatctc caccattatt tctaaacaaa
     1801 caatccccta tttttcaata gaggattgtc ttacgatatt tggaggcaat ttacctataa
     1861 atcatactcc gttcaactga gtaggatacg atttgctact cctacctaga cttttttcta
     1921 gtcctaagtc tttctaggca gattgacgaa ggtgaagcta aatcactaag gaatgatttt
     1981 ctaaacgaga caaggcaaag cgacgtagac ataactaaag ttaggcaagg agctttaacg
     2041 atgtatcgtg acgaaaatcc ttacttgatg cgacctggac gttctttgta accgtagtag
     2101 gcatcttcga tgatttcttg catgtggtca accattgcta gacgtgggtt agctggggta
     2161 cattgatctt cgtaggcaag aagagccaat tcgcgagagt gttctttgag ttctttctcg
     2221 tcgatacctt gtgctttcaa gttcatttgg ataccgatgc gttcaccgag gtcataaact
     2281 gcttgtgcgt atgctgcaac agcttcttct ggtgttgaac atggcaaacc tagaagacga
     2341 gcgatgtctt ggtatttttc atctgcttta tagtagttgt acttaggcca tgtagctgtc
     2401 ttagctggac gagtaccgtt gtagcggata acgtacggaa gcaagatagc gtttgtacga
     2461 ccgtgaacgg tatggaaacg tccaccaatc ttatgagcca ttgagtgaga aatacctagg
     2521 aatgcgttgg cgaaggccat acctgccata gttgatgcgt tgtgcatttt ctcacgtgat
     2581 acaaagtcag cattcttcac tgagctttca aggttttcaa atactagttt gatagcttga
     2641 agtgctaaac catctgtaaa gtcgttggct actgttgaaa cgtatgcttc tgtagcgtga
     2701 gtcaatacgt ccataccagt atccgctgta acgtgtgcag gtactgtcaa tacgagtgct
     2761 gggtcaacga ttgccacagt tggtgtcaat gagtagtcag cgattgggta tttacggttg
     2821 tttgctttat cagagattac ggcaaacggt gttacttctg aacctgtacc tgatgtagtt
     2881 ggaatcgcga taaacttaga tttcttacca agttctggga acttgaaggc acgtttacgg
     2941 atatccatga atttttgaac taggtcatgg aaatcaactg ttggttgttc gtagaagagc
     3001 cacataactt tggcagcatc cattggtgaa ccaccaccga gtgcgatgat tgtatctggt
     3061 ttgaaggtac gcatcaattc tgtaccttta taaaccgttg tgatgtctgg atctggctca
     3121 acgtctgaga agatttgata aacaacttta ttacgacgaa ggtccaattg ttcgatgata
     3181 cgatctagga atccaagttc taccatggca cggtctgtaa cgatcatgac acgttcaacg
     3241 tcacggcatt tttggagata ttggatagag tcacgttcga agtatgtttt agaaggaact
     3301 ttaaaccatt gcatattatt tctacgtctt cctactttct tgatgttgag caagttcata
     3361 gcacttacgt tatcactgat tgagttgcgt ccgtaagaac cacaaccaag tgtcaatgat
     3421 ggcaagaatg cgttgtaaac atcaccgata ccaccgaaag tagatggaga gttccaaata
     3481 acacggatag cacgtacgat tgtaccaaac tctttcgcca attctgcgtc ttccgtatga
     3541 atagctgcag agtgaccgag accgtggaat tcaaccattt ggcgagctgc ttcaagacct
     3601 tctgcacgac cttcaacttt caagacggca ataacaggtg acaatttctc acgagtcaat
     3661 ggttcttttt caccgatttc agcaacctca gctgccaaga tgtttgtacc ttctggtact
     3721 gagaagccgg cctgctcagc gatccatgca gctgggcgac caacgatgtc tgcgttcaat
     3781 ttaccttcag cacagttttt gctgttagct ttcacaccga agcagtattc ttcaagaaga
     3841 gctttttctt tcttattaac gaagtaagtt ttgtaagatt tgaactctgt tacaaactca
     3901 tcgtaaactt ctttatcaat gataactgct tgttctgacg cacagaccat accgttgtca
     3961 aatgacttag acataacgat gtcatgtgct gcctgacgaa tatttgctga tttttcaaca
     4021 taggctggaa cgttacctgc acctacacca agggcaggtt taccacaaga gtaagctgcg
     4081 cgcaccattg cgttaccacc agttgcaagg attgttgcaa taccatcatg tttcatcaac
     4141 tcagatgttg cttccataga tggttttgta atccactgga tacagttttc aggagcacca
     4201 gctgccacag ctgcttcata gacaacacga gctgcatgag cagaagattc ttgtgcagat
     4261 gggtggaagg caaagacaat cggattacgt gtcttcaagg caatcaatga tttaaagatt
     4321 gctgtagaag ttgggtttgt tgttggtgtg ataccacaaa caacaccgac cggctcagca
     4381 atcaaagtca aacctgtcac ttcatcttct tcaataacac caacagtttt cgtgtgacgc
     4441 atgttattga caacgtgctc acaagcaaac aagttcttag tagccttgtc ttcgaataca
     4501 ccacgcttag tttcttcaaa ggcgtgcatt gccaattcgc catgtttatc aagggctgca
     4561 accgaagctt tcgctacaat atagtctact tcttcttgac caagtttacg gaactcatca
     4621 agagcaacca aacctttttg aacaagctca tcgacgtgct tgcgcgcttc taccaacttt
     4681 tcttctggtg atactactat tttatcagcc attgtgttcc tccaaatata ctcgctaact
     4741 atttgttaac tttttcacaa tgttattcta ctatttttta ttttatttgt aaagggaaat
     4801 tgttaatttt ttcacatttt ttctgtttcg ttatagaaag cgcttacttt ttcctatttt
     4861 cagaaaacat tcagatttgt ttctgctttg tttggtcaaa aaatacataa agtaagacaa
     4921 atctttatca tttattcttt ctctaacatt tacgatacta gttta
//
LOCUS       DASFSW010000135         4884 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.136, whole genome shotgun sequence.
ACCESSION   DASFSW010000135 DASFSW010000000
VERSION     DASFSW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.136"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            709..2622
                     /gene="ltrA"
                     /locus_tag="U1099_001800"
     CDS             709..2622
                     /gene="ltrA"
                     /locus_tag="U1099_001800"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM4868090.1"
                     /translation="MTKPKNDDKLLKRQKLRYAEYYGMTEIFDDLYSKSQNGFNFKNL
                     MNIITSPENILLAYRTIKRNGGSQTEGVDGVTIKDLENLTQEDFISKVQRRFQHYRPR
                     KVRRVEIPKPNGKKRPLGIPSMWDRIAQQCILQVLEPICEAKFYKHSYGFRPLRSADN
                     AIMDCMYRMNKSHMTYVVDVDIKGFFDEVNHTKLMRQIWTLGIRDKQLLVIIRKMLKA
                     PIVLPNGKVSHPTKGTPQGGILSPLLANINLNEFDWWIGRQWEERDCKELVPQYNSKG
                     TRHKSHVYRKLRASTTLKEMYIVRYADDFKIFCRNRNEAERTFKAVKLWLKERLNLPV
                     SEEKSQITNLRKKSSEFLGITLKLVSKNNRFVCFSHVAPKAKKRIKHQLKQQMKLIQI
                     KGVKETIIREIQKYNSMVIGIHNYYSMATHVSKDLEEIHYQLYLSFKNRLQAKGLTKI
                     GEYKGKDKGVLPYIQSQNIRYLMGYPILPISYVKTRTLKGRNKNLNRYTPEGRKLIHK
                     QQESVETWKLKWLREHPIINKRATVEYNDNRISLFVAQKGKCAVTGQELDMDDIHCHH
                     KRPWSETKDDSYRNLIIVGRDSHRLIHATNEKAIKNLFQLLNLSNTGLTKLNQLRELV
                     GNPPLLKENLNLE"
     gene            3014..3607
                     /locus_tag="U1099_001801"
     CDS             3014..3607
                     /locus_tag="U1099_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEM4868091.1"
                     /translation="MDLLEKPVYNYIKKNHGVITFRDIEELNFSYQQLNQLVSKGKVE
                     SIERGIYHLPDTYIDDFFSLQYRFPKGIYSLDTALWLHGLSLTVPFEPVMTFPFGTNT
                     RLMKEAGVKPIVARNHHEIGIIKLERQAGQFISVYDMERTLVECLRTAYHVDVQVVAP
                     AFQAYFKKGAVNYAKLYHYAQLFKVTEKLQSYVEVLS"
     gene            3604..4446
                     /locus_tag="U1099_001802"
     CDS             3604..4446
                     /locus_tag="U1099_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEM4868092.1"
                     /translation="MKFSNANSFKAKIKNIAREKGIPAQQVQQNYLIEKILKLISESR
                     YKDSFIVKGGFLIGQMIGLDKRTTMDLDVTLKGQPLSEENIQTIFKEIVSQPSEGFQF
                     EVDMLEPIRQDDEYGGFTLKLKATFDTLREVVFIDITTGDRITPREITYQLQSVFSEN
                     KLEVWTYNLETVLAEKLETIIRRGAASTRPRDRYDLFTLYHLRKDEIDIPILKTALNN
                     TAKKRESLDVLTNWETQLEEIHSSDYQKQLWSRYQKSFRYASEISFEESVEVVAIILK
                     HLGI"
BASE COUNT         1695 a          750 c         1005 g         1434 t
ORIGIN      
        1 ctgaaaaggt tagacacgtt cttgaaaaat caacctgata atcaggttaa atttgaagag
       61 aatggagaac tcttatcttg aaaattggaa tgaaggggta acgccctgaa acgatttctc
      121 taatactccg acatgcgttt atctgatgca aaggtaaagg tatgaagctc ggtgaagtcg
      181 gctgaaagat accgtcattc ttaaggactt aagaatcgaa agtgttccag aggtgggata
      241 tgtatctgat aggtcgggag tcattaaaaa ctaaataggg tgagaatgtg catgagcact
      301 gaagaaccag cgaatgtacg ctccgaaggc gagtacgtag aaatgcgtat agcaacgtaa
      361 gttgtttcga cttgaggaag agtaagaatc gacgatatga aattccttgt tatgttacag
      421 gcatatcaaa gttgaagggg tatagctagg cacctaagtt tagtattgat aaagataagg
      481 gaacgtcgaa agctagagat gtggaggttg cacaaccagc gaagcaatgg aaaggaaatc
      541 ataactttcc ttaatctcta gtgagagtgg tggcacgacc tttgaaagtg ttgtaatgat
      601 actggaggaa cagccactag tcaatagtgt attgttatga actttatcat ttcatggatt
      661 cttgtaagac ataaaaggtc acgaatccaa agagaggagt gatagaccat gacgaaaccc
      721 aaaaatgatg acaaactatt aaaacgtcaa aaactaaggt atgcagaata ctatggcatg
      781 accgaaatat ttgacgattt atattccaaa agtcaaaatg gattcaactt taagaattta
      841 atgaacatta tcacatctcc cgaaaatata ctactcgctt atcgtaccat aaagcggaat
      901 ggtggtagtc aaaccgaggg tgtagatggt gttaccatta aagacttaga aaatttgacc
      961 caagaagact ttatctctaa agttcaaaga agatttcaac attatagacc cagaaaggtc
     1021 agaagagtag agattcctaa accaaatgga aagaaaagac cactaggcat tccctcaatg
     1081 tgggatagga ttgctcaaca gtgtatttta caagtgttag agccaatatg tgaagctaaa
     1141 ttttacaaac atagttatgg atttagacct ctacggtcag ctgataacgc tattatggac
     1201 tgtatgtatc gtatgaataa aagtcatatg acttatgtcg ttgacgttga tattaaaggc
     1261 ttttttgatg aagtgaatca tactaagtta atgcgtcaaa tctggacctt aggcatcagg
     1321 gataaacaac ttctagtgat tattcgaaaa atgttaaaag caccaattgt tcttccgaat
     1381 ggaaaagtga gccatccaac taagggcaca ccacaaggag gtatcctatc tccgctactt
     1441 gccaatatca atcttaatga atttgattgg tggataggac gacaatggga agaaagagat
     1501 tgtaaggaat tagttcctca atataattcc aagggaacaa gacacaagtc acatgtttat
     1561 agaaagctgc gagcttcaac gacactaaaa gagatgtata ttgttcgata tgcggatgat
     1621 tttaaaatct tttgtcgaaa taggaatgag gcagagcgaa cgtttaaggc tgtaaaattg
     1681 tggttaaaag agcgacttaa ccttcctgtt tcggaggaaa aatctcaaat taccaatctg
     1741 agaaagaaaa gcagcgaatt ccttgggatt actttaaagc tggtttctaa gaataatcgt
     1801 tttgtctgtt tttctcatgt tgcaccgaaa gccaagaaac gtattaaaca tcaactgaaa
     1861 caacagatga aattgataca aattaaaggt gttaaagaaa caattattcg tgaaatacag
     1921 aagtataata gtatggttat cggtattcat aactactata gtatggcaac acatgtgagt
     1981 aaggatttag aagaaattca ttatcagctt tacctctctt ttaaaaatcg attgcaggcg
     2041 aaaggtctga caaaaatagg agaatacaag ggaaaagata aaggggttct cccttacatt
     2101 caatctcaaa atattcggta cttaatgggt tatccaattc ttccaattag ctatgtcaag
     2161 acgaggacat taaaaggaag aaataagaat ctgaataggt atactcctga ggggagaaaa
     2221 ttgattcata aacaacagga atctgttgaa acatggaaat taaaatggct gagagaacac
     2281 ccaattataa ataagcgtgc aacagttgaa tataacgata atcgaatttc tttatttgta
     2341 gctcaaaaag ggaaatgtgc tgtaaccggt caggaattgg atatggatga tattcattgt
     2401 caccataaga gaccttggtc agaaacaaag gatgactctt ataggaacct tatcattgtt
     2461 ggacgagata gtcatcgcct tattcacgca acaaatgaga aggctatcaa aaatctcttt
     2521 cagcttttga atttgagtaa tacaggactg accaaattaa atcagttgcg tgaacttgta
     2581 ggaaatccac cattattgaa agaaaatttg aacttagaat aatcatgtta atcatattaa
     2641 tatagaacga taaagcaaaa tcgtgataat acactattga tggaacgccg tgtgcggtga
     2701 aagtcgcacg cacggtgtga agcgggggaa aagatggaga tagtatcaaa gttttaccta
     2761 tcgctataga aagtttatcc agatggatct ttccttatct cagaaaccaa ctataatggc
     2821 aatcctaatt atactttccg taagttatca ggagtcgatg gtacgattag ttttgcctat
     2881 acgacgaaat agaaaatgat atgctaacaa atcgttatgt tgttagcata ttgttttatt
     2941 tgtgattgat tttttctaaa taatatgcta acattgtagt gaaatgttag catattgttt
     3001 aggaggtggt aatgtggatt tgttagagaa acctgtctat aattacataa aaaaaaatca
     3061 tggagttata acttttcgtg atatagaaga gcttaacttt tcttaccaac aactgaatca
     3121 attggtctca aaaggtaaag tggaatcaat tgaaagagga atttatcatc tgcctgatac
     3181 ctatattgat gatttcttta gtttacagta tcgttttcca aaaggaattt attccttaga
     3241 cacagcactt tggttacatg gcctttccct tactgttcca tttgaacctg taatgacttt
     3301 tccttttggg acgaacacga ggctaatgaa agaagctggt gttaagccga ttgttgctag
     3361 aaatcatcat gaaatcggta ttataaaact agagcgacag gctggacagt ttatctcagt
     3421 atatgatatg gaacgaactt tggtagagtg tttgagaaca gcttatcatg ttgatgttca
     3481 agtcgttgct ccagcgtttc aagcttactt taaaaaagga gctgtgaatt atgccaagtt
     3541 gtaccactat gcacaactat ttaaagtcac tgaaaagcta caatcttatg tggaggtctt
     3601 gtcgtgaaat tttcaaatgc caatagtttc aaagcgaaaa ttaagaatat cgcaagagaa
     3661 aaagggatac ctgctcaaca agttcagcag aactatttga ttgaaaaaat cttaaaactc
     3721 atctctgaaa gtcggtataa agattctttc attgtgaaag gtggattcct tatcggacag
     3781 atgattggct tagataaacg aacaactatg gatttagatg ttactttgaa aggtcaacct
     3841 cttagcgagg aaaatattca aacaatcttc aaagaaattg taagtcaacc ttccgaaggt
     3901 tttcaatttg aagttgatat gttagagcca attcgacaag atgatgaata tgggggcttt
     3961 accctcaaac tcaaggcgac attcgatacc ctacgtgaag ttgtctttat tgatataaca
     4021 acaggtgatc ggataacgcc acgagaaatt acttatcaat tgcagtctgt tttttcagag
     4081 aataaactag aagtttggac ttataattta gaaactgttc ttgctgaaaa attagaaacg
     4141 attattcgtc gaggagctgc ttcaactaga cctcgtgacc gctatgacct cttcacatta
     4201 tatcaccttc gaaaagatga gattgatatc ccaatattga aaactgctct aaacaatact
     4261 gctaagaaaa gagaaagctt agatgttttg acaaattggg aaactcaact agaggaaatc
     4321 catagctctg attaccagaa acagctttgg tctcgctatc aaaaatcgtt cagatatgcc
     4381 agtgaaatta gttttgagga atcagtggaa gtggtggcaa ttattttaaa acacttaggg
     4441 atttagtaat gcatgtttgg ccaggtgaag ccaaattcgt agaagaagat attctttatg
     4501 gccggacaaa tgaaacaaat aatttgatgt tatagagtgg ctaaaaaata gtataatcca
     4561 aaagtttagt attactgatt ttgtaggcct tgggttcatt gctttataat agttttgtaa
     4621 tgatatatat cgatataaaa ccgtcgattc gacaaataag taaacttatt gatgaaaaac
     4681 aaagttagtg gtagtataac tatatcgaat tggggcgtgc tagtcacgtc tgggagggtc
     4741 tgatacgtca gtcctcccct tttttatagg gattttcaga agttatcggt agtgctactg
     4801 atagcttttt ttctttgtct ttttttgatg tgataccaaa atcaagtcgt cttttttatg
     4861 atgaggagag agtgacagat tgtt
//
LOCUS       DASFSW010000136         4825 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.137, whole genome shotgun sequence.
ACCESSION   DASFSW010000136 DASFSW010000000
VERSION     DASFSW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.137"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            225..1382
                     /locus_tag="U1099_001803"
     CDS             225..1382
                     /locus_tag="U1099_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEM4868093.1"
                     /translation="MLKTKKAKIALFGGLGVVAVALVGGALIFSNMLTSPSSSEDVAM
                     SMTYTVAKEGSIASSTLLTGTITAADEQYVYYDPSKGDLSEVLVAPGAKVEVGTPLIR
                     YDATELQSALDTAVRGRDKIGRQIADLRTNGQTVQTTGDAETDAAATAAAQRSVDMQL
                     ADLNDSYADAQAAVDKAYTALTEATVTSTVAGTVVEVNKAVSKNSTSSQTVVHIVNQG
                     SLQVTGSLTEYDLANIAVDQEVKLTSKVYPDKTWTGKITYISNYPSSNQAGAATTGTS
                     GSGAKYPFKAALTSELGELKQGFSVNIEVVNTSKNILIPVSAVVPEEDKNYVWTLVDG
                     KAKKVEVTLGNADALNQEVTAGIAAGDKVITIPTPNLEDGKEVEANEEPAY"
     gene            1363..2058
                     /locus_tag="U1099_001804"
     CDS             1363..2058
                     /locus_tag="U1099_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868094.1"
                     /translation="MKNQLIRLTSINKSYKNGDQELRVLKNIDLEVEEGEFLAIMGPS
                     GSGKSTLMNIIGLLDRFSSGNYWLEGEEVSQLSEKKLAQVRNDQIGFVFQQFFLLSKL
                     NALQNVELPLIYAGVPSSKRKELAKQYLEKVELAERMTHLPSELSGGQKQRVAIARAL
                     VNTPAIILADEPTGALDTKTGEQIMQLLTELNNEGKTIIMVTHEPEIAAYAKRKIVLR
                     DGVITEDSRREGV"
     gene            2059..3306
                     /locus_tag="U1099_001805"
     CDS             2059..3306
                     /locus_tag="U1099_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4868095.1"
                     /translation="MENWKFALKSIMAHKMRSLLTMLGIIIGVAAVVIIVAMGTGVAK
                     SIEKSLAGDQNNVQVYFTAFSSSGTAFGPFGTASTEEPILEEEPDLTDSTLKGLLEID
                     GVSNYYSSISNIAEVRSGNKKAENVTITGVSQNFFAVKEYEILAGRQFTANDYSRFSR
                     MIMLDTALAEKLFGSIENSLNQTISVNSNAYLVIGVYKDPKAGTAIYGMNSGGNAVMT
                     NTQLAAETGMKENSQIFVHVEDVTRATEVGKAAADYLTKATGLKEARYEIYDMSAMLE
                     EFKSQMSGVTLFIGAVAGISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRGNILI
                     QFLIEAMVLTTLGGAIGLAIAQTIVFLLNVSKALGERIAAEISIPVVLGSLAFSAVVG
                     IVFGVLPANKASKLDPIEALRYE"
     gene            3432..4496
                     /locus_tag="U1099_001806"
     CDS             3432..4496
                     /locus_tag="U1099_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEM4868096.1"
                     /translation="MVELGISTFGETTPLEKTGETYSHDERIHQLVKEIELADAVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVNTKHIKLTSAVSILSSMDPVRLYQQYTTIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLHDYEELFDEKLDMLLEIDKETNLKWDGHFTQSV
                     DNKPVYPRPVQEDFPIWVATGGNVESTIKIAKKGLPIVYAVIGAGAHRFKPLVDAYRK
                     VARNSGHDPKKTKVAAHSWGWIADDHDKAVEEYYHPTKVITDNIAKDRPHWSEMTKAQ
                     YLYSLTDEGATIVGDPKRVAEKIIKTIETLDLDRFFLHLPIGSMPHEDVLRAIELYGK
                     EVAPIVRDYFAKKEENQSQD"
BASE COUNT         1460 a          888 c         1111 g         1366 t
ORIGIN      
        1 ttacgctatt ttgaaatgaa ctagcaccac gggttaacta tattcattgt aacgcttttt
       61 tgtgtgtcaa tcaaaaatcc gctacgttac cttacaaaaa tgtaagtggg aaaattgccg
      121 aactttttat aaaatagtta ggaaaaaact tgcatttgtt cttgtatttg tgtattatat
      181 agacaacaca aataggtatg ttttgtaaag aaaaggagtt tgttatgtta aagacgaaga
      241 aagcaaaaat tgctttgttt ggtggtcttg gggtagtcgc agttgcctta gtaggtgggg
      301 ctttgatttt tagcaatatg ctaacatcac catcatcttc agaagatgta gccatgagta
      361 tgacctatac tgttgctaaa gaaggatcaa ttgcttcctc aacgctactt acaggaacga
      421 ttacagcagc agacgagcag tatgtttact atgatccttc taagggagat ttgagtgagg
      481 ttttggttgc tccaggtgct aaggtcgaag taggtacccc tttgattcgc tatgatgcta
      541 cagaattaca gtctgctttg gatactgctg ttcgtggtcg tgataagatt ggtcgccaaa
      601 ttgcagactt aagaaccaac ggtcagactg ttcaaacaac tggtgatgca gaaacagatg
      661 ctgcagcaac tgctgctgca caacgttcag tagatatgca gttggcagac ttgaatgatt
      721 catacgcaga tgctcaagct gcggttgata aagcatatac agccttaaca gaagcgacag
      781 taactagtac cgttgctggt acagttgttg aggtgaataa ggctgtttct aagaattcta
      841 catcgagcca aacagttgta cacattgtaa atcaaggtag tttgcaagta acaggtagtt
      901 tgacagaata cgatttggct aacattgcag tggatcaaga ggtcaagctg actagcaagg
      961 tatatcccga taagacttgg acagggaaaa ttacctatat ttcaaactat ccatctagta
     1021 atcaagctgg tgctgctaca acaggaacca gtggttcagg tgccaaatat ccatttaagg
     1081 cagctttaac tagtgaatta ggtgagttga aacaaggatt ttctgtaaat attgaggttg
     1141 tcaatacaag taaaaacatt ttaattccgg tgtccgcagt tgtgccagaa gaagataaaa
     1201 actatgtgtg gaccttggtt gatgggaaag ctaagaaagt agaagttact ctggggaacg
     1261 cagatgcttt gaaccaggaa gtgaccgctg gtatagcagc aggagataag gttattacca
     1321 ttccaactcc gaatctcgaa gatggcaaag aggttgaagc caatgaagaa ccagcttatt
     1381 agactaacaa gtatcaataa atcctacaaa aatggtgatc aagaactccg agttttaaag
     1441 aatattgatt tagaggtgga agagggagaa tttcttgcca ttatggggcc ctctggttca
     1501 gggaaatcaa ccttgatgaa cattattggg cttttggatc gtttcagttc aggaaactat
     1561 tggctggaag gtgaagaagt cagtcaactt tctgagaaaa aattagctca ggttcggaat
     1621 gaccaaatcg gtttcgtctt tcaacaattt ttcctcttat cgaaacttaa cgcccttcaa
     1681 aatgtagaat tacccttgat ttatgctgga gttccatcaa gcaaacgtaa ggaactagca
     1741 aaacagtatc ttgaaaaagt tgagttggca gagcggatga cgcatttacc atcagaattg
     1801 tctggtggtc aaaaacaacg ggtagctatt gctcgtgcac tagtcaatac cccagctatt
     1861 attttagcgg atgaacctac aggggctcta gataccaaga caggtgagca gattatgcaa
     1921 cttctgacgg agttgaataa tgaaggaaag accattatca tggttacgca tgagcctgag
     1981 attgctgcct acgcaaaacg aaaaatcgtt ctacgtgatg gagtgatcac tgaagatagt
     2041 cgaagggagg gagtttagat ggaaaattgg aaattcgccc tcaaatctat tatggctcat
     2101 aaaatgagat cgctccttac tatgttaggt atcatcattg gtgtggctgc agttgtcatt
     2161 attgtcgcca tgggaacagg ggtggccaaa agcatagaaa agtctctagc tggtgatcaa
     2221 aataatgttc aggtttactt tactgctttc tccagttcag gtacagcttt cggtccgttt
     2281 ggcacagcgt cgacagaaga accaatttta gaggaagagc cggatttgac ggattctact
     2341 ttaaaaggac tgctagagat tgatggcgta tcaaactatt actccagcat cagcaacata
     2401 gctgaagtca gatcgggcaa taaaaaagca gagaatgtca ctattactgg tgttagccag
     2461 aatttctttg ctgtgaaaga gtatgagatt ttagctggtc gtcagtttac agctaacgac
     2521 tatagtcgtt tttcacgtat gattatgtta gatacggcat tagcagagaa gttatttggt
     2581 tctatagaga actctctcaa tcagacgatt agtgttaata gcaatgccta tctagtaata
     2641 ggggtctaca aagatccgaa agcaggcaca gctatttatg gaatgaattc aggtggaaat
     2701 gccgtcatga ctaatacgca gctagctgct gaaacaggca tgaaagaaaa ttctcaaatc
     2761 tttgtccatg ttgaagatgt tactcgggca acagaagttg ggaaggctgc tgcagattac
     2821 ctgactaaag cgactggatt gaaagaagcg agatacgaga tttatgatat gtcagctatg
     2881 ttggaggagt ttaaatctca aatgtctggc gttacattgt ttatcggtgc tgttgcaggt
     2941 atctccttgc tagttggtgg tatcggtgtc atgaatatca tgctggtatc agtaactgag
     3001 cggactcgtg aaatcggtct tcgaaaagcg cttggagcaa ctagaggaaa tatcttgatt
     3061 caattcttga tagaagccat ggtcttgacc actcttggtg gagcaattgg tctcgcaatc
     3121 gctcagacaa tagttttctt gctcaatgtg agcaaggcgt tgggcgaaag aatagctgct
     3181 gaaatttcaa ttcctgttgt gttaggtagt ctggctttct cagcagtagt tggtattgta
     3241 tttggcgtac tacctgccaa caaagcatct aagcttgatc caattgaagc tttgcgctat
     3301 gaataatctg ttgaaacgat taggttttcc tagtcgtttt attttttgag caaatcaatt
     3361 gattttatca ctaattagag ataaaataaa cgcatcaatt aagaaatagt gatttcaaag
     3421 gagaacacat catggtagaa ttaggtattt caacatttgg cgaaacaact ccactagaaa
     3481 aaacagggga aacttacagc cacgacgagc ggattcacca gctagtcaaa gaaatcgaac
     3541 tagctgatgc ggtggggctt gatgtttatg ggattgggga gcatcaccgc gaagattttg
     3601 cggtttctgc acctgagatt gtgctggcag ctggtgccgt caataccaag catatcaaac
     3661 taacatcagc agtttcaatc ctttcctcca tggaccctgt aaggctctat cagcagtaca
     3721 ccaccattga tgctttgtca aatggcagag cggaaatcat ggcgggtcgt ggttccttta
     3781 cggagtcatt ccccttgttt ggctatgatc tccacgacta tgaagaactc ttcgatgaaa
     3841 agctagatat gcttctggaa atcgacaagg aaaccaatct caagtgggat gggcatttta
     3901 cccagtcagt cgataataag ccagtctatc cacgtcctgt tcaggaagat tttccgatct
     3961 gggttgcaac tgggggcaat gtagagtcca ccatcaagat tgctaagaaa ggcctgccaa
     4021 tcgtgtatgc agtcattggt gcaggtgccc atcgctttaa acctttagtc gatgcttatc
     4081 gcaaggttgc tcgcaactca ggtcatgatc caaagaaaac gaaagttgca gcccattctt
     4141 ggggttggat tgcagacgac catgacaagg ctgtggaaga atactatcat ccaactaaag
     4201 taattacgga caatattgcc aaagaccgcc cacattggag cgagatgacc aaggcgcaat
     4261 atctctactc tctgacagac gaaggagcaa ccatcgtcgg agatccaaaa cgagtcgctg
     4321 aaaaaattat caagactatt gaaacacttg acttggaccg tttcttcctc catctcccaa
     4381 tcggctcaat gccacatgaa gatgtcctcc gtgctatcga actctacgga aaagaagttg
     4441 cgccaattgt ccgagattat tttgcaaaaa aagaagagaa tcagtcccaa gactgatgag
     4501 ataaaaagga ggggacgtta taatagcctc acttctctaa agtggagtca acatctcagc
     4561 gcagtggttg attggaagat ttgttcgtgt tttacactcc acatcttcct aatcaactgt
     4621 gcgggggtgg gaagacgaac tctctatgat tatttgagtt ctgtcccact cccaatccta
     4681 aatatttttc ataatctcct gaaaatcctg accatatggt tgggattttt tgaatgatta
     4741 gatagtgacc ggtatgtaaa ttatttcata gttgaaagtt aacttagatg atttgtttat
     4801 tcttttaaag tgaatattta tataa
//
LOCUS       DASFSW010000137         4816 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.138, whole genome shotgun sequence.
ACCESSION   DASFSW010000137 DASFSW010000000
VERSION     DASFSW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.138"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1144)
                     /gene="icd"
                     /locus_tag="U1099_001807"
     CDS             complement(<1..1144)
                     /gene="icd"
                     /locus_tag="U1099_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEM4868097.1"
                     /translation="MAEKILMREGRLIVPDCPVIPYIEGDGIGRDIWSVSQKVIDAAV
                     KKAYAGQRSIQWREVLAGEKAYELTGTSLPNETLEIIREHLVAIKGPLGTPIGKGHRS
                     LNVALRQELDLYACVRPIRYFKGVPSPLKEPEKTDITIFRENTEDIYAGIEWEAGSDE
                     VAKVISFLQEEMGVVNIRFPESSSIGIKPISEEGSKRLIRSAIEYALEHQLKIVTLVH
                     KGNIQKFTEGGFRKWGYELAKEEFATELAEGTLVVNDIIADNFLQQILLAPENFQVVA
                     LTNLNGDYASDALAAQVGGIGISPGANINYQTGHAIFEATHGTAPDIAGKDLANPCSL
                     LLSACMMLEYIGWKEAATLIIQALEDAFINKNVTLDFAKILDVAHQS"
     gene            complement(1147..2265)
                     /locus_tag="U1099_001808"
     CDS             complement(1147..2265)
                     /locus_tag="U1099_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEM4868098.1"
                     /translation="MTETNGLKDAIACDTRISAIENDRLSYAGYDIAELMENNASFEE
                     VIYLLWNLHLPTQIELNYFEKSLRKEYAISDAVEQCILIQSRRHLHPMSVLRSTVSLL
                     GVYNVHAEENSLEALYEQSIQIMGKVPTIIATFARLRRGLVPIEPRSDLGFAANFLYM
                     LNGEEPSDLQIKAFNKALVLHADHELNASTFAARVTASTLTDLYSCVTTAIGTLKGSL
                     HGGANERVFDMLLAIRESGDAERYLQKKLDSKEKIMGFGHRVYKTVDPRQEYLKEMAR
                     DLTFGTDDEAFYRLSEEVESFIKNKKGLIPNVDFYSATVYHVLGIDSDIFTLIFAMSR
                     MAGWIAHVQEQRKNNKLIRPRSSYLGGRNLVYIPIGKR"
     gene            complement(2262..>4816)
                     /gene="acnA"
                     /locus_tag="U1099_001809"
     CDS             complement(2262..>4816)
                     /gene="acnA"
                     /locus_tag="U1099_001809"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEM4868099.1"
                     /translation="RILLESLLRKEDGVDVTKNHIMELLHYQAASPKGEIPFKPSRVI
                     LQDFTGVPVVVDLASMRDAVVKAGGNPELINPEIPVDLVIDHSVQVDFFGTEDALEKN
                     IALEFERNNERYEFLKWAENSFENYRAVPPATGIIHQVNIEFLSDVIINKDGLLYPDS
                     MFGTDSHTTMINGIGVLGWGVGGIEAEAAMLGEASYFPVPEVIGVRLAGQLPKVATAT
                     DLALKVTQLLRQENVVGKFVEFFGPGLSSLALADRATVSNMAPEYGATCGYFPIDGET
                     LHYMRLTNRSEEHVELTEAYTKANYLFYDAERFPSYSKVLELDLSTVVPSISGPKRPQ
                     DLIELTDAKAEFQASLIREVGVRGFGLEEAELDKTATVKYVEGDEQIQTGHVAIAAIT
                     SCTNTSNPYVLLAAGLLAKNAVEKGLAVSKTVKTSLAPGSKVVTGYLKKSGLQTYLDA
                     LGFNLVGYGCTTCIGNSGDLRPEVAEAIKEEDLLVSAVLSGNRNFEGRINPLVKANFL
                     ASPPLVVAYAIVGNMNVDLTRDPLGYDEKQQAVYLADIMPSREEVDDYIERYVTRDLY
                     KEEYQQVFTDSQAWNAIETKTDKNYNWNSSSTYIQNPPYFDNMQVDLSIKPLENLSVL
                     AKFGDSVTTDHISPAGNIARLSPAARYLEENGIVYKDFNSYGSRRGNHEVMMRGTFAN
                     IRIKNELAAGKIGGWTRVGDEILPIYDAAMKYKAAGIGSIVIAGKDYGMGSSRDWAAK
                     GSSLLGVKAVLAESFERIHRSNLVMMGVLPLQFLEGQSAESLGLTGHESYTIDLPEDV
                     GVGQIVTVHAQIDDVTKEFQALVRFDAEADIRYYRHGGILPMVVRKKLGGKV"
BASE COUNT         1447 a         1206 c          792 g         1371 t
ORIGIN      
        1 tagattgatg agcaacgtcc aaaattttag caaaatcaag cgtaacattt ttatttataa
       61 aagcgtcctc caaggcctga ataattaaag tagccgcttc tttccagcct atatattcta
      121 acatcataca agccgaaagc aagagggaac atggattagc caaatccttt cctgcaatat
      181 caggagcagt tccatgtgtc gcttcaaaga tagcatgacc tgtctgataa ttaatattag
      241 cacccggtga gatgcctatt cctccgacct gagccgcaag agcatctgat gcataatctc
      301 catttagatt tgtaagagca accacttgga agttttccgg cgccaataaa atctgctgca
      361 gaaagttatc cgcaataatg tcatttacta ccaaagtccc ctctgcaagt tctgtagcaa
      421 attcttcttt cgccaattca taaccccact tgcggaaacc gccttctgta aacttttgaa
      481 tatttccctt atgaactaag gtcactatct tcaattgatg ttctagggca tattctattg
      541 cagagcgaat gaggcgttta ctcccttctt ccgaaatcgg tttgatacca atacttgaag
      601 attctggaaa acggatattt accactccca tttcttcttg cagaaaggaa atgactttgg
      661 caacctcatc tgaaccagcc tcccattcaa tacctgcata aatatcttct gtattttcac
      721 gaaaaatagt aatatccgtc ttttcaggct ctttcagcgg acttggcact cccttaaaat
      781 agcgaatcgg gcgcacacag gcatacaaat ctaattcttg acgaagtgca acattaagag
      841 aacgatgacc tttcccaatc ggcgttccca agggaccttt gattgcaact aaatgctcac
      901 ggattatttc cagtgtctca ttcggcaaag aagttcccgt taattcataa gccttttctc
      961 ctgccaaaac ctccctccac tgaatagaac gttgacctgc ataggctttt ttcactgcag
     1021 catcaataac tttttgactg actgaccaaa tatccctgcc aataccatct ccttcaatat
     1081 agggaataac gggacaatct gggacaatta accgtccctc tctcattagg attttttctg
     1141 ccatatttac ctctttccaa tcgggatgta aaccaaattc ctaccaccta agtaggaaga
     1201 gcgcggacga atcaatttat tatttttccg ttgttcctgg acatgtgcaa tccaccccgc
     1261 catacggctc attgcaaaaa tcaatgtaaa aatatcacta tcaataccta gcacatggta
     1321 aactgtcgcc gaataaaaat ctacgtttgg aattaaacct tttttgtttt taataaagct
     1381 ttctacttcc tctgacaatc tataaaacgc ttcatcgtct gttccgaatg tcaaatcacg
     1441 cgccatctcc ttcaaatact cttggcgagg atcaaccgtt ttatagactc gatggccaaa
     1501 tcccataatt ttttccttcg aatccagctt cttctgcaaa tatctctcgg catcaccact
     1561 ctcgcgaata gccagcaaca tgtcaaacac tcgttcattt gcaccaccat gcaatgagcc
     1621 tttcagagtc ccaattgccg tggtaacaca agaatataaa tccgttaacg ttgatgccgt
     1681 gacacgcgca gcaaaagtag atgcattcaa ttcatgatca gcatgaagca ccaaagcttt
     1741 attaaacgct ttgatttgta aatcactcgg ctcctcacca ttcagcatat aaagaaaatt
     1801 cgccgcaaaa cccagatcag accgcggttc aattggaacc aatccgcgtc tcaaacgagc
     1861 aaatgttgca ataatcgtcg gaaccttgcc cataatctga atagattgtt catacaaagc
     1921 ttctaacgaa ttctcttcag catgtacatt atatactcca aggagagata ctgtcgaacg
     1981 caacacgctc ataggatgca aatgtcgccg tgattgaatc agaatacatt gctctaccgc
     2041 atctgaaatc gcatattctt ttcgcagact tttctcaaaa taatttaatt caatctgagt
     2101 tggcaaatgc aggttccaca gaagatagat gacttcctca aaagaagcgt tattctccat
     2161 caattcagca atgtcatacc ctgcatagga caagcggtca ttttcgattg cactgattcg
     2221 cgtatcacag gcaatcgcat cttttaaccc attcgtttct gtcatacctt tcctcctagt
     2281 ttctttctta ctaccatagg taaaatacct ccgtggcgat aataacgaat atccgcctct
     2341 gcatcaaacc gcaccagagc ttgaaactcc tttgtaacat cgtctatttg cgcatggact
     2401 gttacaattt gaccaacacc gacatcttct ggcaagtcga ttgtatagct ttcatgtcca
     2461 gtcaagccaa gactttctgc cgactgacct tctaaaaatt gtaaaggaag aacacccatc
     2521 atcactaaat ttgaacggtg aatccgctca aatgactcag caagaacagc cttcacacca
     2581 agtaaacttg aacctttggc tgcccaatca cggcttgacc ccatcccata gtctttacca
     2641 gcaataacaa tactaccgat accagcggcc ttatatttca tggctgcatc atagatagga
     2701 agaatctcat cgccaactct tgtccaacca ccaatttttc cagctgccag ctcattcttt
     2761 atacgaatat ttgcaaaagt cccacgcatc atgacttcat gatttccacg acgacttcca
     2821 taagaattaa aatccttata aacaatgcca ttctcttcga gataacgagc tgcaggactc
     2881 aatcgtgcaa tattccctgc cggggaaata tgatctgtcg ttacagaatc gccaaattta
     2941 gccaaaacag ataaattttc cagtggtttt atggataaat ctacctgcat gttgtcaaaa
     3001 tagggcggat tttgaatata ggtagaagat gaattccaat tgtaattctt atctgttttt
     3061 gtttcaatcg cgttccaagc ctgactatct gtaaatacct gttggtattc ttccttataa
     3121 aggtcacgag tcacataacg ctcaatgtag tcatctacct cctcacgact tggcataatg
     3181 tccgcaaggt agacagcttg ttgcttctca tcatacccca gcggatccct tgttaaatct
     3241 acattcatat ttcccacaat agcataggca acaacaagcg gtgggctcgc taagaaatta
     3301 gctttaacca atggattaat ccgtccttca aagttacgat ttccagataa taccgcagaa
     3361 accaataaat cttcttcctt aatcgcctct gctacttcag gacgcagatc acctgagtta
     3421 ccgatacaag ttgtacaacc ataaccaacc aaattgaacc ctaaggcatc tagataggtc
     3481 tgcaaacctg attttttcaa atagccagtc actacttttg agcctggtgc caatgacgtc
     3541 ttgaccgtct tagaaactgc caaacctttt tctaccgcat tttttgccag taatcctgcc
     3601 gccaaaagga catacggatt ggaggtattt gtacacgagg taatcgccgc aatagcaaca
     3661 tgacctgttt gaatttgctc atctccttca acatacttaa ccgtggctgt tttatctaac
     3721 tctgcttcct ccaaaccaaa tccacgcaca cctacttcac gtattaaact agcctgaaac
     3781 tctgcttttg catctgttaa ttctatcaaa tcctgtggtc ttttaggacc agaaatagat
     3841 gggactactg tcgataaatc caattctaaa accttgctat aagacggaaa acgttcagca
     3901 tcatagaata aataattagc cttggtatag gcttctgtca actccacgtg ctcctccgaa
     3961 cggttggtca atctcatata atgtaaagtt tctccatcga tcgggaaata gccacaagtc
     4021 gcaccgtatt ctggagccat atttgaaaca gttgctcgat ccgctaatgc aagagaagat
     4081 aaacctggtc caaaaaattc gacaaacttc ccaactacat tttcctgacg tagaagttga
     4141 gtgaccttaa gcgccaaatc cgttgcagtg gcaaccttag gcaattgccc agctaggcga
     4201 acaccaatca cctcaggaac gggaaaataa gatgcttcac ccaacattgc agcttccgcc
     4261 tcaataccac ctacacccca accaagtaca ccaataccgt taatcatggt cgtatgactg
     4321 tctgtaccaa acatagaatc tggataaagc aaaccatcct tgttgataat aacatcgctt
     4381 agaaattcaa tgtttacctg atggataatt cctgtcgctg gaggcacagc gcgataattt
     4441 tcaaaggaat tctctgccca tttcagaaat tcatagcgtt cattatttct ttcgaactct
     4501 aaggcaatat ttttctccag agcatcttca gtcccaaaaa aatcaacttg aacagagtgg
     4561 tcgatgacta aatccactgg aatttccggg ttaatcaatt cagggtttcc acctgctttc
     4621 accaccgcat cacgcatgga ggctaaatct accacaacag gaacacctgt aaaatcttgc
     4681 aaaataacac gactaggttt aaaggggatt tcccctttag gagatgcagc ctgataatga
     4741 agcaactcca ttatgtgatt ttttgtaaca tccacaccat cttcttttct taaaagactc
     4801 tctaataaaa tacgaa
//
LOCUS       DASFSW010000138         4720 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.139, whole genome shotgun sequence.
ACCESSION   DASFSW010000138 DASFSW010000000
VERSION     DASFSW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.139"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..356
                     /locus_tag="U1099_001810"
     CDS             <1..356
                     /locus_tag="U1099_001810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM4868100.1"
                     /translation="ELEKLKKQVQYDLLKESGCVEKLCEWDEVLPIIADVFYQNEGTI
                     SINQMRLPIDFVKERFRQLNMHHIDYICECLSKVDTNIRNIRAYILTTAFNAPQTIGA
                     YYSNQVKQDFGGSIL"
     gene            353..2947
                     /locus_tag="U1099_001811"
     CDS             353..2947
                     /locus_tag="U1099_001811"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEM4868101.1"
                     /translation="MISKITKFCQRKLTIGLVSLSIGFISVNSIILSPNNLFTVYANE
                     VVSHRNDITGSSKEWGTLAGNQFIIDYTNTSFEVSKAAHSSFSENYHAIRIPETYPYK
                     LFVNGQPKTLSDLKRDKIGHLTAVIDGREVPLLDYGMVEYQMNRYLGKDIYNIYGNLS
                     YTAYAFLTTRTSHHGYFELTEADNVRDVSGQLVTGFFQTPEYRDMASAYHKSNSGYLK
                     VQVGDQELSTNIVNGRLNGLKTAKQHFYLVSNSEFSMDPQTGKPMKQLRELTEDEVHQ
                     LKVTETKVNSEIEFDVNGKTYQTTGRLNFWKNSTINPLKTRLAVMSIGDGNNIHGFDL
                     YASNNNYIEVVEKIAEEPSIPESISKMIEFVTEDGEKVSDGPSITGNKGEEVTLEITP
                     PDGYTFVSIPPERIVLEDSNPIQIIVNENTGQETLERVEEIDYRVIRQENPDMYVGDE
                     AIVRKGIRGTKTIQDIFETKKGIRTDKLLSSSVISETAPSDEIIHYGTKAIEGEEIIT
                     RTEITPFNIIEEVDPNLYEGEIVTKVEGQNGEFTYYDYYRTIKGTKELAAFKSEAKEI
                     KPKIDKVVRIGTKQIEGEIIEKSSEKIPFGMIIEEDNSLFEDERVVIREGVPGIVDVV
                     ATYKTSKGEKIGEPINVQSFIQREAVDELVKVGTKPLNTTSKVENLNEIPFTVVKEYD
                     DSLEEGVEKIIKVGKNGQKKIIVTTNYVRGIQDGESIVEEITIEEPQNQVILVGTKTL
                     QKESPSPNNDNTDSQTKIDIPSSDDNLPKVPKIPNNTQSDIEKPIEKETKDSSEINQK
                     GSSQTDVNKSKTTTKLDSNTTNNTNGSKLPETGETLVSPLLIIISSIFCLFTSLGMII
                     WKGRKSND"
     gene            2940..3434
                     /locus_tag="U1099_001812"
     CDS             2940..3434
                     /locus_tag="U1099_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEM4868102.1"
                     /translation="MINEEVGNRLVTLNLRLAKSSAKMIIAALKLVIKEAESEKLSVK
                     QYISQKSLQGEVPLKDLVGKGTLENLPMKQPELLELKKELNKHGVRFSVLRNKENKQF
                     EVFFQAKDTAVLEHSFKQAVERAERKTKIKQSTLKKIQQYQKSLGQHIIKPKIKQKHQ
                     EQSL"
     gene            3446..>4720
                     /locus_tag="U1099_001813"
     CDS             3446..>4720
                     /locus_tag="U1099_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEM4868103.1"
                     /translation="MEVLKQIKQDILNVFPFGDRRLLAKSLLLYLLFSYLGTCLGHHV
                     NNFSDGDFLDRLFQAILEIETMRWIFFPSLDEIITGLTLSVVVGIVLYFRKKNAKKFR
                     NGVEYGSARWGDRKDIQPYEDPVFENNILLTDSEKLTMNSRPSNPKFARNKNILVVGG
                     SGSGKTRFFVKPNLMQMHSSYCVTDPKGTIVLECGKMLEKNGYQIKILNTINFKKSMK
                     YNPFAYLKSEKDILKLVQTIIANTKGEGEKAGEDFWVKAEKLYYTALIGYIYYESPKE
                     EQNFNTLLAMIDASEVREDDENFKNAIDYLFLALERRKPNHFAVKQYKKYKLAAGKTA
                     KSILISCGARLAPFDIQELRELMSDDELELDTLGDRKTALFVIISDTDDTFNFVVSIL
                     YSQLFNLLCDKADDVYGGRLPVHVRCLLDEFAN"
BASE COUNT         1744 a          701 c          923 g         1352 t
ORIGIN      
        1 atgagctaga aaaattaaaa aaacaagttc agtatgactt attgaaagaa tcaggttgtg
       61 ttgagaaatt atgtgaatgg gatgaggtgc tacctatcat tgctgatgtg ttttaccaaa
      121 atgaaggcac aatctctatc aatcaaatga gattgccgat agattttgta aaagaacgtt
      181 ttagacaatt aaatatgcac catattgact atatctgtga gtgtttgtca aaggtagata
      241 caaacattcg taatatcaga gcctatattc taacaacagc atttaatgca cctcaaacaa
      301 taggtgctta ttattcaaac caagtaaaac aagattttgg aggaagtata ttatgatttc
      361 aaaaataact aaattttgcc aacgtaaatt aactattgga ttggtttcac tttccattgg
      421 tttcattagc gtaaattcta ttattcttag tcctaataat ttattcacag tttatgctaa
      481 tgaagtggta tctcatcgaa atgatataac tgggagtagt aaagaatggg gtactttagc
      541 tggaaatcaa ttcattattg attatacaaa tacgagtttt gaagtttcta aagcagcaca
      601 ttctagtttt tcggagaatt atcatgctat tcgaattcca gaaacttatc cttacaaatt
      661 atttgtcaat ggacagccaa aaacattatc tgatttaaag cgtgataaaa taggtcatct
      721 aactgctgtt atagatggca gagaagtacc tttgcttgac tacggtatgg tagagtatca
      781 gatgaatcgc tacctaggta aggatattta taatatttac ggtaatctat catatactgc
      841 atacgcattt cttacaactc gtacttccca ccatggatac tttgaattaa ctgaagcaga
      901 taacgtaagg gatgttagtg gtcagttagt aactggattc tttcaaacac ctgagtaccg
      961 tgatatggca tctgcctatc ataaatcaaa ttcaggatac ttaaaagtac aagtgggtga
     1021 tcaagaactt tctacgaata ttgttaacgg tcgattgaat ggactgaaaa ctgccaaaca
     1081 acatttttat ctagtatcaa atagtgaatt ttcaatggat ccacaaactg gaaaaccaat
     1141 gaaacaatta agggaactaa ctgaagatga agtacatcag ttgaaagtaa ctgaaacaaa
     1201 agtaaactca gaaatcgaat ttgacgtaaa tggtaaaaca tatcagacaa ctggacgatt
     1261 aaatttttgg aaaaactcga ctattaatcc attaaaaaca cgacttgcag ttatgtcaat
     1321 tggtgatgga aataatatac atggttttga tttgtatgcc tcaaacaaca attacatcga
     1381 agttgtagaa aagatagcgg aagaaccctc aattcctgag tctatttcta aaatgattga
     1441 atttgttact gaagacggtg aaaaggtaag tgatgggcca tctattactg gtaacaaagg
     1501 agaagaggtc acattagaga ttaccccacc tgatggatat acatttgttt caataccccc
     1561 tgaaaggatt gtattagagg acagcaatcc aattcaaatc attgttaacg agaatacagg
     1621 tcaggaaaca ttagaaagag tagaagagat agattaccgt gttattcgac aagaaaatcc
     1681 tgatatgtat gttggagatg aagctattgt tagaaaagga ataagaggca caaagactat
     1741 tcaagatatt tttgaaacaa agaaaggtat cagaacggat aagcttttaa gctcaagtgt
     1801 tatttctgag actgccccaa gtgatgaaat aattcactac ggcacaaaag cgattgaagg
     1861 tgaggaaata atcacaagaa ctgaaattac accatttaat atcatcgagg aggtagatcc
     1921 aaatctatac gaaggtgaaa ttgtaacaaa agtcgagggt caaaacggtg agtttactta
     1981 ttacgattat taccggacaa ttaaaggtac aaaagaactc gccgctttca aatcagaagc
     2041 taaggaaatt aagccaaaaa ttgataaggt cgtaagaatt gggaccaagc aaattgaagg
     2101 tgagataata gaaaaatcaa gcgagaagat tccatttgga atgattattg aagaagataa
     2161 ttctttattt gaggacgaac gtgtcgttat tagagaagga gtccctggaa tcgtagatgt
     2221 ggtagcaaca tataaaacta gtaagggtga aaaaatcgga gagccaatta atgttcagtc
     2281 atttattcaa cgtgaagcag ttgatgaatt ggtcaaggtt ggtacaaagc ctttaaatac
     2341 cacttctaaa gttgagaatt tgaatgaaat tccttttact gttgtgaaag agtatgatga
     2401 tagccttgaa gaaggtgttg agaagataat caaagttggg aaaaatggac agaagaaaat
     2461 tattgtgaca acaaattatg tacgtgggat acaagatggc gaatctatcg tagaagaaat
     2521 tacgattgaa gagccacaga atcaagttat cctagtaggc acaaaaacat tacagaaaga
     2581 gtccccttcc cctaataatg ataatacaga cagtcagact aagatagata ttccatcatc
     2641 agatgataat cttcctaaag taccaaaaat accaaataat acgcagagtg atatagaaaa
     2701 accaatcgag aaagaaacta aagatagttc agaaattaat cagaaaggtt ctagtcaaac
     2761 agatgtaaat aaaagtaaaa ctacaacaaa attggatagt aacaccacaa ataacacaaa
     2821 tggttcaaaa ttgccagaaa ctggagaaac acttgtaagt cctctgttga ttatcatcag
     2881 tagtattttt tgtttattca cttcattggg gatgattatt tggaagggac ggaaatcaaa
     2941 tgattaacga ggaggttggc aatcgtcttg taacactaaa tctaaggtta gcaaaaagtt
     3001 ccgccaaaat gataattgca gctttaaagt tagttattaa agaagctgaa tcagaaaaat
     3061 tatcagtcaa acaatacata tctcaaaaaa gtttgcaagg ggaagttccc ttaaaagatt
     3121 tagttggaaa gggaacgtta gaaaatttgc ctatgaaaca gcctgaactt cttgaattga
     3181 aaaaggaact aaataagcat ggtgttcgat tctcggtatt gaggaataag gagaataaac
     3241 agtttgaggt gttttttcag gctaaagata cggctgtctt agaacattct tttaagcagg
     3301 cagttgaacg tgcagagaga aaaacaaaaa ttaagcagtc aactcttaag aaaatacaac
     3361 aatatcaaaa atcacttggc caacatatca taaaaccaaa aatcaaacaa aaacatcagg
     3421 aacaatcctt ataggaggtg gtaggattga agtattaaaa caaatcaaac aagatattct
     3481 taatgttttc ccattcggag ataggagatt actcgcaaag agtttattac tctatctcct
     3541 tttttcgtat ctcggtacct gtctaggaca tcatgtcaat aacttttctg atggggattt
     3601 ccttgatagg ttatttcaag caatcctaga aattgaaacg atgcggtgga tattctttcc
     3661 tagccttgat gaaataatta ctggcttaac cttgtcagtt gttgttggaa tagtacttta
     3721 ttttagaaaa aagaatgcaa aaaagtttag gaatggagtg gaatatgggt ctgccagatg
     3781 gggtgatagg aaagatattc agccatatga agatccagta ttcgagaata atattctttt
     3841 gacagatagt gagaaattaa ccatgaattc tcgaccgtcg aatccaaagt ttgctcggaa
     3901 taaaaatatc ttggttgtcg gagggtctgg tagtggaaag acaagatttt ttgtgaagcc
     3961 gaatctaatg caaatgcatt ctagttactg tgtcacagat ccaaaaggaa caatcgttct
     4021 tgaatgtggc aaaatgttag aaaaaaatgg ctatcagata aaaatactga atactattaa
     4081 cttcaaaaag tcaatgaaat ataatccttt cgcttatcta aaaagtgaga aggatattct
     4141 taaactagtt caaactatta tagccaacac caagggagag ggagaaaaag cgggagaaga
     4201 tttttgggtc aaagccgaga aactctacta tacagccttg attggttata tctactatga
     4261 aagtccaaaa gaagaacaaa actttaatac cttattggcc atgattgatg ctagtgaagt
     4321 tcgggaagat gatgaaaatt tcaagaatgc cattgattat ttatttctgg ccttggaaag
     4381 aagaaaacca aatcactttg cggtcaagca atacaaaaag tataaactag cagcaggtaa
     4441 aactgccaaa tctattctca tatcatgtgg tgctagattg gcaccttttg atattcaaga
     4501 gttgagagaa cttatgagcg atgatgagtt ggaattggat actctgggag atagaaaaac
     4561 tgccctattt gtcatcattt ctgatacaga tgacaccttt aactttgttg tgtccatctt
     4621 gtattctcag ttatttaatt tactttgcga taaagcagac gatgtttatg gtggtcgtct
     4681 accagtccat gttcgctgtc tgctggacga atttgcaaac
//
LOCUS       DASFSW010000139         4596 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.140, whole genome shotgun sequence.
ACCESSION   DASFSW010000139 DASFSW010000000
VERSION     DASFSW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.140"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..263)
                     /locus_tag="U1099_001814"
     CDS             complement(<1..263)
                     /locus_tag="U1099_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730450.1"
                     /note="acts to negatively regulates ftsZ ring formation by
                     modulating the frequency and position of the ftsZ ring
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEM4868104.1"
                     /translation="MPTGTIILIVSIVIILIIAYVACLIVRKRNDNLLVALEERKEEL
                     FNLPVNEEVEAVKALHLIGQSQVSFREWNQKWVDLSLNSFADI"
     gene            complement(352..2304)
                     /gene="gyrB"
                     /locus_tag="U1099_001815"
     CDS             complement(352..2304)
                     /gene="gyrB"
                     /locus_tag="U1099_001815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEM4868105.1"
                     /translation="MTEEIKDLQEKAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFADKIEVYIEPDNSITVIDNGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVYKNGQVYFQEYKRGEVV
                     ADLEVVGETDRSGTTVHFTPDPEIFTETTEFDFAKLNKRIQELAFLNRGLNLSITDKR
                     EGVEQTKEYHYEGGIASYVEYLNENKDVIFETPIYTEGEMDDITVEVAMQYTTSYHEN
                     VVSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPGPQFEGQTKTKLGNSEVVKITNRLFSDAFAEFLLENPQIARRIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDATKTELFIVEGDSAGGSAKSGRDRE
                     FQAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSEIKEYIQPGVNQEQE
                     LQDALERHSTGRSKPTIQRYKGLGEMDDHQLWETTMNPENRLMARVSVDDAAEADKIF
                     DMLMGDKVEPRREFIEENAVYSTLDV"
     gene            complement(2486..3025)
                     /locus_tag="U1099_001816"
     CDS             complement(2486..3025)
                     /locus_tag="U1099_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEM4868106.1"
                     /translation="MTRVAVVLADGFEEIEALAPVDVFRRAHFDCQIVGLDSHEVEGS
                     HGIRVQTDQVFDGDLSSFDLIVLPGGMPGSVHLRDSESLITELQRAVASGKSVAAICA
                     APIVLDKAGLLDSRHYTCFPGKEKDIPSGILLEEDVVVDGPIITSRGAGTSLDFAYKL
                     VDLFGGDGESLAQTMVYHS"
     gene            complement(3039..4268)
                     /locus_tag="U1099_001817"
     CDS             complement(3039..4268)
                     /locus_tag="U1099_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM4868107.1"
                     /translation="MRKLMKNRGTIDSRVDYSLILPVFFLLVVGIISLYIAVSQDYPD
                     NVVQMVGQQIAWIGIGTVAAFLVMFFSTKFLWQITPYLYVLGLGLMVLPLFFYSPDLV
                     ASTGAKNWVTIGGMTLFQPSEFMKIAYIIMLSRVIVTFHKYYPNRKIREDFMLIGYMT
                     LFTIPVLILLALQKDLGTSLVFVAIFSGMLLLSGVSWKILLPTALTGIVLVGGFMLIF
                     ISPGGTTFLHNLGMDTYKINRIAAWLDPFKNAQSTTYQQAQSLIAIGSGGLKGLGFNK
                     TNLLIPVRESDMIFTVIGEDFGFIGGTVLIGLYLLLIYRMLRVTLKSNNRYYTYISTG
                     YIMMLLFHVFENVGAATGLLPLTGIPLPFISQGGSSMVSNLIGVGLVLSMGYQSRLAD
                     EKETNRSRRYRKITIKR"
BASE COUNT         1416 a         1057 c          782 g         1341 t
ORIGIN      
        1 tcaatgtcgg caaatgaatt gagcgacaaa tcaacccatt tttgattcca ttcacggaat
       61 gacacctgac tttgaccaat caaatgaagt gctttgacag cttcgacttc ttcattaact
      121 ggaaggttaa atagctcttc cttacgttct tccaatgcaa ccaaaaggtt gtcatttcgt
      181 ttacgaacaa tcaggcaagc tacataggca atgattaaaa taataacaat cgaaacgatt
      241 aagatgattg ttcctgtagg catgtaaatc tccttagctc atagtaatta ctagaaaata
      301 tcgcttaatt ataccataaa tctctagtat atgcacttgt ttttttagtt tttagacatc
      361 caatgttgaa taaacagcat tttcctcgat aaactctcga cgaggttcaa ccttatcccc
      421 cattaacatg tcaaaaatct tatcggcttc tgccgcatca tcaactgaaa cgcgtgccat
      481 gagacgattt tcaggattca tcgtagtttc ccacaactga tgatcatcca tttctcccaa
      541 acctttgtaa cgttggatcg ttggcttgga ccgtcccgta ctatgtcttt ctaaggcatc
      601 ttgcaattct tgctcttgat taaccccagg ttgaatatat tcttttatct cacttccaac
      661 cttgataccg taaataggtg gttgagcgat atatacgtat cccgcttcta caacaggacg
      721 catgtagcgg taaaataatg tcagaaggag tgtccgaata tgggcaccat cgacatcggc
      781 atctgtcata atgactaatt tttgataacg tgccttgcta acgtcgaaat ccgcaccaaa
      841 gccagttccc atagctgtaa atagactacg gatttcttcg ttggccaaaa tcttgtccat
      901 gctggctttt tctacgttca aaatcttacc acgaatcggt aaaatagcct gaaattcacg
      961 gtcgcgaccg gatttagctg aaccaccggc tgagtccccc tcaacgatga aaagctctgt
     1021 tttcgttgca tcatttgagg aacagtctgc taacttacct gggaggtttg agatttcaag
     1081 acccgatttc ttacgagtta cctcacgcgc acgcttagca gcaatacgcg ccttgcttgc
     1141 caaaatccct ttttcaacaa tccgtctagc aatctgtgga ttttccaaaa gaaattctgc
     1201 aaaggcatca gagaaaaggc ggttggtaat tttgaccact tcactattcc caagttttgt
     1261 cttagtttgt ccctcaaatt gaggacctgg gtgcttaaca gaaatgaccg ctgtcaagcc
     1321 ttcacgcaca tcttcaccag tcaaattatc ctcattttct ttgagaattt tatttttctt
     1381 ggcgtagtcg ttgataacac gtgtcagagc tgtacggaat ccttgctcat gggtaccacc
     1441 ttcatgtgtg tgaatattgt tggcaaaact aactacgttt tcatgataac tcgtcgtata
     1501 ttgcatggca acctcaacgg taatatcatc catttcacct tctgtataga taggggtctc
     1561 aaaaatgaca tccttgttct cattaagata ctcaacgtag gaagcaatac caccttcata
     1621 gtgatattcc tttgtttgct ctactccctc acgtttgtcc gtaatagaga gatttaaacc
     1681 acgatttagg aaggccaatt cttggatccg cttatttaat tttgcaaagt cgaactcagt
     1741 cgtctcagta aaaatttctg gatcaggtgt aaaatgtacc gttgtaccag aacggtctgt
     1801 ttctccaaca acctccaaat ctgcaacgac ttcacctcgt ttatactctt ggaaatatac
     1861 ctgaccattt ttgtagacat ggacatctag ctgagtagat agggcgttta cgactgatga
     1921 acctacgccg tgcaatccac ctgagacctt ataaccgcct ccgccaaatt taccacctgc
     1981 atggagcact gtaaagacag tttctaccgc tggacgacca gttttttcct gaatatctac
     2041 cggaatacca cgtccattat caataaccgt gatggagtta tctggctcaa tataaacttc
     2101 aattttatcc gcaaaaccag ccagcgcctc atcgattgag ttgtcgacaa tttcccaaac
     2161 caagtggtgg agaccttctt tagatgttga gccgatatac atccctggac gcattcgaac
     2221 agcttcaaga ccttccaaga cttgaatctg actggcatca tattcttgag ccttctcttg
     2281 taaatctttg atttcttctg tcatttcttt tccttatttt ctatttgttg ctccttaaaa
     2341 atagaaaagc caatccatct ggataaaagc gaaacatatt tttagagcaa aaatcattct
     2401 aacgaatacc ctttattata ccatattttc taatattttg atagaaaaaa gaagaggaaa
     2461 cattagctcc atcttcttca ttactttaac tatgataaac cattgtttga gctaggcttt
     2521 ccccatctcc accaaataaa tcaaccaatt tataggcaaa atccaaactt gttccagcac
     2581 ctcggctagt gataattgga ccatcaacga ccacatcctc ttccagaaga atacctgacg
     2641 gaatgtcttt ttctttccct gggaaacatg tatagtgacg actatctaac aaaccggcct
     2701 tatccagaac aattggagcc gcacagattg ctgctactga ttttcctgat gcaaccgctc
     2761 tttgcaattc cgtaattagt gactctgaat ctctaaggtg aacagaacct ggcattcctc
     2821 ctggtaaaac aatcagatca aaggaagaca ggtctccatc aaagacttgg tcagtttgaa
     2881 cacgaatacc gtgcgatccc tccacttcat gactatctaa accaacaatc tgacagtcaa
     2941 aatgagctcg acggaataca tctacagggg ccaatgcttc gatttcttca aaaccatcgg
     3001 ctaaaacaac tgcaactctt gtcatataaa ccaactttct aacgttttat cgtaattttt
     3061 ctatagcgac gtgatcgatt tgtttccttc tcatcagcta gtcgtgactg ataccccatt
     3121 gacagcacta gaccaacacc aatcaaattt gaaaccatgg acgagccacc ttgggagata
     3181 aacggcaaag gaatacctgt taaaggcaac aaacccgttg cagcgccaac attttcaaac
     3241 acatggaaca acagcatcat gatatagcca gttgatatgt aggtgtagta tcgattgttt
     3301 gacttcaacg ttacccgcag cattcgataa atgagcaaca agtatagtcc aatcaatacc
     3361 gtcccaccaa taaaaccaaa gtcctctcca atcaccgtaa aaatcatatc actttctcga
     3421 acaggtataa gaagatttgt tttattgaaa cctagtcctt ttaggccacc acttccgata
     3481 gctatcaaac tttgagcttg ctggtaggtc gttgattggg catttttaaa gggatcaagc
     3541 caggctgcaa tacgattgat tttataagta tccatcccca aattgtggag aaatgttgtt
     3601 ccaccaggag aaataaaaat cagcataaat ccaccaacca aaacgatccc cgttaaggca
     3661 gtcggtaata gaattttcca agaaactcca gataatagaa gcattccgct aaagattgct
     3721 acaaatacta aggaggttcc taaatctttc tgtaaggcca acaaaatcag gacggggata
     3781 gtaaacagtg tcatataacc aatcaacata aaatcctcac gaatttttcg atttggataa
     3841 tatttatgaa aggtgacaat cacccttgac aacataatga tgtaggctat tttcataaat
     3901 tcggatggtt gaaaaagagt cattccacca atcgttaccc agttttttgc tcccgttgac
     3961 gctactaagt ctggactgta gaaaaacaaa ggcaatacca taagtcctag cccaagtacg
     4021 tacaagtaag gtgtgatttg ccacagaaac ttagtggaaa agaacatgac caaaaaagct
     4081 gccactgttc caattcctat ccaagcaatt tgttgaccaa ccatctgtac aacattatcc
     4141 ggataatctt gactaaccgc aatgtagaga gaaataatcc ccacaactaa taaaaaaaag
     4201 accggtaaaa taagtgagta gtctactcga ctatcgattg ttcccctatt tttcatcaat
     4261 ttcctcatac tttcacttca aattcttact cgttccatta tagcagaaat catctctcat
     4321 tgaaactaaa aatctagtta catcagcggg gatacgattt atactcaacg aaaatcaaaa
     4381 gtagcctagg aaacgaagtc gaagatagaa ctggagttca tcaaggcaag ttgacaacgg
     4441 agaattttga ttttcgaaga gtattactag tgcacaaatc ataaaatacg tatatcttca
     4501 taccttcgaa caggctgaaa tataatgctt taccttcaca caccccataa gtttgctcaa
     4561 ttcatgagta tgtaaaccaa tggcaatcat tttggc
//
LOCUS       DASFSW010000140         4544 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.141, whole genome shotgun sequence.
ACCESSION   DASFSW010000140 DASFSW010000000
VERSION     DASFSW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.141"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..705
                     /locus_tag="U1099_001818"
     CDS             <1..705
                     /locus_tag="U1099_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEM4868108.1"
                     /translation="LFNKQPNLLKAIPSLIASRDKTLDILSIDADDNMGFDNLDFAKI
                     DEDRIVDYTNFVEATGLLDFLQKKANRSLVDYVYGVEAGLDSNARKNRSGTTMEGILE
                     RTVARVCKEYGLEFKAQATAPFIKDNWGIEVPVDKSERRFDVAVFSKDKHKLWLIETN
                     YYGGGGSKLKAVAGEFTELSQFITKSTDDVNFVWVTDGQGWKTAHLPLAEAFGHITHV
                     FNLDMLKQGYLHDLFR"
     gene            826..2088
                     /gene="purD"
                     /locus_tag="U1099_001819"
     CDS             826..2088
                     /gene="purD"
                     /locus_tag="U1099_001819"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEM4868109.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESEQVEQVFVAPGNDGMTLDGIEL
                     INIGISEHSALINFAKENDIAWTFVGPDDALAAGIVDDFEQAGLKAFGPSRLAAELEW
                     SKDFAKQIMVKYGIPTAAFGTFSNFEEAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAREMLLDNKFGDSGARVVIEEFLAGEEFSLFALVNGDQFYILPTAQDHKRAF
                     DGDQGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPILEGMITEGRSYLGVLYAGLILT
                     DQGPKVIEFNARFGDPETQIILPRLTSDFAQNIDDILHKRPTQLTWLNSGVTLGVVVA
                     SNGYPLDYEKGVTLPAKTEGDITTYYAGARFAENSRALLSNGGRVYMLVTTADTVQDA
                     QEKIYSELKNQDTTGLFYRTDIGSKAVK"
     gene            2114..2602
                     /gene="purE"
                     /locus_tag="U1099_001820"
     CDS             2114..2602
                     /gene="purE"
                     /locus_tag="U1099_001820"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEM4868110.1"
                     /translation="MNIPISIIMGSSSDWKTMKKAADVLDKFGVAYEKKVVSAHRTPD
                     LVFRHAEEARGRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVQSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDSGIADQLASFAIEQGKIAEAMSDE
                     LN"
     gene            2589..3674
                     /gene="purK"
                     /locus_tag="U1099_001821"
     CDS             2589..3674
                     /gene="purK"
                     /locus_tag="U1099_001821"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEM4868111.1"
                     /translation="MNSTKTIGIIGGGQLGQMMAISAIYMGHKVITLDPAADCPASKV
                     SEVIVAPYHDVAALKQLAERCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNC
                     IFEKDFLAKKAGVQVAPYKVVTSSLDLEGLDLSKNYVLKTATGGYDGHGQKVIREAAD
                     LEAASQLANSTECVLEEFVNFDLEISVLVSGNGSDYTVFPVQENIHRNNILYKTIVPA
                     RISDELAEKAKTMALQIADKLHLAGTLCVEMFVAGQEILVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHIRGILGEPLPPIRLLSPAVMINVLGQDMEAVQTFLQENPTAHAHFYGKLE
                     AKHNRKMGHVTVLTEDVEVGFRQDEIR"
     gene            3661..>4544
                     /locus_tag="U1099_001822"
     CDS             3661..>4544
                     /locus_tag="U1099_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HEM4868112.1"
                     /translation="MKLDRLKEVDVREIWKHEQYDFSEWLSRTENIELLSDEIGLTLT
                     EIDKEVFVGSYRCDLVAIDETTGIKVIIENQLELTDHDHLGKIITYASGLDAEVVIWI
                     VKKAREEHRSAIEWLNNNTTKNISFFLIEIHAYQIGSSMPAPKFVLVEKPNDFTKLAK
                     GNNHGGDLSKAQAERLYFWTQFNNVVATKNKPFNVRKATTDHWYDVAIGTSEAHISIT
                     LVNKSKSVGIEIYINNNKELFDKLSSHADEIHEELGFEMEWKRLDNRKVSRIIYYIKG
                     LDFENHDNYNDLIDEVIN"
BASE COUNT         1295 a          924 c         1123 g         1202 t
ORIGIN      
        1 ctttttaata aacaaccaaa tttattaaaa gctataccaa gtttgattgc aagtcgtgat
       61 aaaacactgg atattttatc aattgatgcc gatgataata tgggttttga taacttagat
      121 tttgcaaaaa ttgatgagga tagaattgta gattatacta actttgtgga agcaacagga
      181 ttattagatt ttttgcaaaa gaaagccaat cgttctttag tggactacgt ttatggagta
      241 gaggcgggac ttgacagtaa tgcacgtaaa aacagaagtg gtactaccat ggaggggatt
      301 ttagagcgaa cagtagcacg tgtctgtaaa gaatatggat tggagtttaa ggcacaagca
      361 acagctccat ttataaagga taattggggg attgaggtcc ctgttgataa gtcggaacgc
      421 cgatttgatg ttgctgtatt ttcgaaagat aaacataaat tatggttaat tgaaacgaat
      481 tattacggtg gaggcggtag caagttaaag gctgtagctg gagaatttac agaattaagt
      541 caattcatca caaaatctac agatgatgtt aattttgtgt gggtaactga tggtcaggga
      601 tggaagactg cccatcttcc tttagcagaa gcatttggtc atataaccca tgtcttcaat
      661 cttgacatgc taaaacaagg ttacttgcat gatttgttta gataatcaag tttgctcacc
      721 tcgtgtgagc tttttccatt cccgaacatt ctcctgtttt ttctattaaa atccgtctgt
      781 ttttgctata ctgtttatag aaaatccgtt tttgaggtgt aagaaatgaa acttttggtt
      841 gtcgggtctg gtggtcgtga acacgctatt gcaaagaagt tgttagagtc tgagcaggta
      901 gaacaggtct ttgttgctcc tggaaatgat ggaatgactt tagacggtat tgaattgatc
      961 aatatcggta tttccgaaca ttctgccctc atcaactttg caaaggaaaa tgatattgca
     1021 tggacttttg ttggtccaga tgatgctctg gcagcaggta tcgttgatga ttttgaacag
     1081 gctgggttaa aggcatttgg tccgagtcgt cttgccgcgg agctagagtg gtcaaaagac
     1141 tttgctaagc aaatcatggt caaatacggc attccaacag cagcctttgg cacattttcc
     1201 aacttcgaag aagccaaagc ctacatcgaa gagcagggtg caccaatcgt ggtcaaggcg
     1261 gacggattgg cactgggcaa gggtgtggtc gtggcggaaa ccgtcgagca ggcggtcgaa
     1321 gcggcacggg agatgctctt ggacaacaag ttcggtgact caggtgcccg cgtggtcatc
     1381 gaggagttct tggcgggcga ggagttttcc ctctttgccc tggtcaacgg cgaccagttt
     1441 tatatcctgc cgacagccca ggaccacaag cgtgcctttg acggagacca ggggcccaac
     1501 actggcggta tgggggctta cgctcctgtt ccccacctgc ctcaaagcgt agtggacaca
     1561 gcggttgaca ctattgtcaa gcctattctt gagggcatga ttacggaagg gcggtcttat
     1621 ctgggcgtgc tctatgctgg cttgattttg accgaccaag gtcccaaggt catcgagttt
     1681 aacgcccgtt ttggcgaccc agaaacccag attattctgc ctcgtctgac ctctgacttt
     1741 gctcagaaca tcgacgacat cctccacaaa cgtccgacac agctgacctg gctgaatagc
     1801 ggcgtgacgc tgggtgttgt cgtggcctca aatggctatc ctttggatta cgaaaaaggc
     1861 gtgaccttgc cagcgaagac cgagggtgac atcacgacct attatgcagg ggctcgtttt
     1921 gcggaaaata gcagagcact gctttcaaac ggcggtcgag tttacatgct agtcaccaca
     1981 gcagacaccg tccaagacgc ccaggaaaaa atttactcgg agctgaaaaa tcaggatact
     2041 acaggcctct tttatcgaac agatattgga agcaaggccg ttaaataagg aacaaagaaa
     2101 aggaaagaca agtatgaata ttccaatttc catcatcatg ggctctagtt ccgactggaa
     2161 aaccatgaaa aaagcagccg atgtgctgga caaatttggc gtagcctatg aaaagaaagt
     2221 ggtctctgcc caccgcaccc cagacctcgt gttccgtcat gccgaagagg cgcgtggtcg
     2281 tggtattaag gttattatcg cgggagcggg tggtgcggct catttgccag gtatggtagc
     2341 agccaagacc accctgcctg ttatcggtgt tcctgtccaa tcccgtgccc tcagcggtgt
     2401 ggattcgctc tattctatcg tgcaaatgcc aggcggcgta cctgtggcaa cgatggcaat
     2461 tggagaagcg ggagcaacca atgcggcttt gactgccctg cgtatcctgt ccatcgaaga
     2521 tagcggtata gcagatcaac tggccagctt tgctattgaa caaggaaaaa tcgcggaggc
     2581 tatgagcgat gaactcaact aaaacaatcg ggattattgg tggcggtcaa ctgggtcaga
     2641 tgatggccat ttctgctatt tacatggggc acaaggtcat cacgctggat ccagcagcgg
     2701 actgcccagc ttccaaggtt agcgaggtta tcgtcgctcc ctatcacgat gtggcggccc
     2761 tcaaacagct ggcggagcgg tgcgatgtcc tgacctacga gtttgaaaat gtcgatgccg
     2821 acggactgga cgcggtcatc aaggacggcc agctccctca ggggacagac ctcctccgta
     2881 tttcccaaaa ctgcattttt gagaaggatt ttttggcaaa aaaagctggc gtgcaagtcg
     2941 ccccctacaa agtcgtcaca tctagcctag acctagaagg tctggacctc agcaaaaact
     3001 acgttttgaa gacagctact gggggctatg atggacatgg tcagaaggtc attcgagaag
     3061 ccgcggactt ggaggcggct agtcagttag ccaactctac cgagtgtgtt ttggaagagt
     3121 ttgtgaattt cgacctggaa atctccgtcc ttgtgtctgg caatggctcc gactacaccg
     3181 tctttcctgt gcaggaaaat atccaccgca ataatattct ttacaagacc attgtgcctg
     3241 ctcgtatttc tgatgaactt gctgaaaaag ccaaaactat ggccctgcaa atcgcagaca
     3301 aactccattt ggcaggcacc ctctgtgttg agatgtttgt ggcagggcaa gaaattcttg
     3361 tgaatgaaat cgctcctcgc ccgcacaatt caggacatta ttcgattgaa gcctgtgatt
     3421 tttcacagtt tgacacccat atccgaggca ttttagggga gcccctgccc cctatccgcc
     3481 tgctttctcc agctgttatg atcaatgtcc tcggacaaga catggaagca gtccaaacct
     3541 ttcttcaaga aaaccctacc gctcatgccc acttttatgg taaactagaa gcaaagcaca
     3601 atcgcaaaat gggacacgtg acggtgttga cggaagacgt tgaggtggga tttagacaag
     3661 atgaaattag ataggctaaa ggaagtagat gttagagaaa tctggaaaca tgagcagtat
     3721 gatttctcgg agtggctgtc taggacagag aatatcgaat tactgagcga tgagatagga
     3781 ctgactctta ccgagattga caaggaagtt ttcgttggct catatagatg cgatttagtg
     3841 gctattgatg aaacaacggg aataaaagtg attatagaaa accaattaga attaacggat
     3901 catgatcatc ttggtaaaat cataacctac gcatcgggct tagatgctga ggttgtcatt
     3961 tggattgtaa aaaaagctag agaagagcat aggagtgcta tcgagtggct taacaataat
     4021 acaacgaaaa atatatcttt tttcctaatt gaaatccatg cttatcaaat aggtagttca
     4081 atgcctgctc ctaaatttgt tcttgttgag aaaccgaatg attttacaaa gctagctaaa
     4141 ggaaataatc acggtggtga tttgagtaaa gcacaggcgg aacgtttgta tttttggact
     4201 caatttaata atgtagtggc aactaaaaat aaaccgttca acgttagaaa agctactaca
     4261 gaccactggt atgatgttgc tattggaaca agtgaggctc atatttctat taccctagtc
     4321 aataaatcaa aaagtgtagg gattgaaatc tatataaaca ataataagga attatttgat
     4381 aagctatcat cacatgctga tgaaatccac gaagaacttg gatttgaaat ggaatggaag
     4441 aggttagata atagaaaggt ttccaggatt atatattata taaagggtct ggattttgag
     4501 aaccatgaca attacaacga tttaattgac gaagtaatta acaa
//
LOCUS       DASFSW010000141         4474 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.142, whole genome shotgun sequence.
ACCESSION   DASFSW010000141 DASFSW010000000
VERSION     DASFSW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.142"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            19..702
                     /locus_tag="U1099_001823"
     CDS             19..702
                     /locus_tag="U1099_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM4868113.1"
                     /translation="MDISKEEKQKILENVSAYNSTLEKGTIPNLNIDSVALKKYDSIL
                     NITDTGIMGYVEIPKLQTMLPIYHGTDDAVLQVAVGHLAGTSLPIGGQGSHAAISGHR
                     GLPSAKLFSDLDQLVIGDTFMIQVLDNTLTYEIDKIITVTPDDVSPLMIDPNEDFVTL
                     VTCTPYGVNSHRLLVRGHRIENQLLARQVVSEGSIVKPILVSPFIALCLLGLLWSLFF
                     VKKEVNLFM"
     gene            833..1009
                     /locus_tag="U1099_001824"
                     /pseudo
     CDS             833..1009
                     /locus_tag="U1099_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1109..1519
                     /locus_tag="U1099_001825"
     CDS             1109..1519
                     /locus_tag="U1099_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM4868114.1"
                     /translation="MNIKEVSDVTGLSADTIRYYERIGLIPKIARKSSGVRNFSESDV
                     AILEFIRCFRSAGMSIERLIEYMGLVQAGDSTVEARIDLLKEEREELRSRLSEIQQAL
                     DRLDYKIENYQTILRGAENQLFTDGGSSFKKGRG"
     gene            complement(1541..1855)
                     /locus_tag="U1099_001826"
     CDS             complement(1541..1855)
                     /locus_tag="U1099_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEM4868115.1"
                     /translation="MALRHYQSHEYQYEEYIPKEKKANFQTYQAKNTNKERLKELLFF
                     VNIAFFSLITVIASYVYLSVGIPVFVAIILASATGFLGLRLVQIGLKKKFKPKQVIRK
                     NE"
     gene            2016..2945
                     /locus_tag="U1099_001827"
     CDS             2016..2945
                     /locus_tag="U1099_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM4868116.1"
                     /translation="MEKIIITATAESIEQVKELLVAGVDRIYVGEADHALRIPTNFTY
                     DELREIAELVHSAGKELTVAANALMHQDMMDNIKPFMELMREIKVDYLVVGDTGIFYI
                     NKRDGYNFKLIYDTSVFVTSSRQINFWKDHGAVEAVLAREIPSEELFDIAKNLEMPAE
                     ILVYGASVIHHSKRTLLQNYYNFTKADETDLSRGRGLFLAEPSDPDSHYSIFEDKHGT
                     HIFINNDIDMMTKLSELDEHGYHNWKLDGIYCPGQNFVEIVKLFVQARDLIEKGEFTY
                     DQAFLLDEQVRKLHPAERGLDTGFYEFDRNKVK"
     gene            3123..4409
                     /locus_tag="U1099_001828"
     CDS             3123..4409
                     /locus_tag="U1099_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM4868117.1"
                     /translation="MSKTLKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFTMDEMREGIEYAHARGAKVYVAANMVTHEGNEEGAGAWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKELGLTRVVLAREVTMDEVAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSKRDANRGGCSQSCRWKYNLYDLPFGEERRSI
                     KGEIPEEFSMSAVDMCMIDHIPDMIENGVDSLKIEGRMKSVHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLIDEMWKVAQRELATGFYYSPPSENEQLFGARRKIPEYKFIAEVV
                     AFDKETMTATIRQRNVINEGDQVEFYGPGFRHFETTIQDLHDSNGEKIDRANKPMELL
                     TIKLDREIYPGDMIRACKSGLINLYQEDGQSLTIRA"
BASE COUNT         1357 a          844 c          982 g         1291 t
ORIGIN      
        1 acttattctg agaaattatt ggatatttca aaagaagaaa aacaaaaaat tttagaaaat
       61 gtcagtgcat ataatagcac gctcgaaaag gggacaatac ctaatttaaa tatagacagt
      121 gtagctttaa aaaagtatga ctcaatttta aatatcacag atactggtat tatgggctat
      181 gtagaaattc ctaaattgca gacaatgtta cctatttatc acggaacgga tgatgccgta
      241 ttgcaggtag cagtaggtca ccttgctggg acttcccttc ccattggcgg tcagggaagt
      301 cacgctgcta tttcaggaca tcggggattg ccttctgcaa aactgttttc tgatttagat
      361 cagctggtta taggggacac atttatgata caagttttag ataacactct gacatacgaa
      421 attgataaaa ttattacggt tactccagat gatgtatctc ctttgatgat agatccgaat
      481 gaggatttcg taacccttgt gacttgcaca ccttatggtg ttaatagcca ccgattatta
      541 gttagaggtc atcgaattga aaatcaacta cttgctaggc aagtagtctc agaaggatca
      601 attgtaaaac ctatactggt ttctcctttt attgctttat gtttattagg actactttgg
      661 agtttatttt ttgtaaaaaa agaagtaaat ttgtttatgt gacttagata gtatcgcaaa
      721 gaatgtcaaa ctattcgact ttgttcttta gcttttaagc ttaggaaagg gagatttttc
      781 caactcccaa tcatccaaaa ggtgaaatag aagataaacc aaatgacgat atgtcaatgt
      841 cctctatacg ctagaaaaag gtggcaagcg agctgtcgga ttcaaattgt cagacggcat
      901 tgacattcct aaggaatttg aaggtaaatt taagtttgcc cgccaacgct ccaaactagc
      961 tggcgaaatt cgtggcacct tctttgttgt caagggcgag tatttatagg atggcaaatc
     1021 tgaggagact cagatttttt aaataatgac ttgacctgga gtggacttca aggtgtataa
     1081 taagcgaaaa caaagaaagg agcctggaat gaatatcaaa gaagttagtg atgtaacagg
     1141 tctatcagca gacaccattc gctactatga gcggatagga cttattccca aaattgcaag
     1201 aaaatcttct ggagtaagga acttttcgga aagtgatgta gccattttgg aatttatccg
     1261 ctgttttagg agtgctggta tgtctattga gcgcttgatt gagtatatgg gcttggtgca
     1321 ggcaggagat agtacagtgg aagctcggat tgatttacta aaagaggaac gggaagaact
     1381 gcggtcacgc ttgtcggaaa ttcagcaagc tctagatcgt ttagattata aaatagaaaa
     1441 ttaccagact atattaagag gtgcagagaa tcaattgttc acagacggcg gtagttcatt
     1501 caaaaaaggc agaggataac ttctctgcct ttatacttca ttattcattt tttcttataa
     1561 cttgtttggg tttaaatttc ttctttagcc caatttgaac caaacgtagt ccaagaaaac
     1621 ccgtcgcact tgccaatatg atggcaacaa aaacaggaat gcctacggat agatagacat
     1681 aactagcgat gactgttatc aaactaaaaa atgcgatatt aacaaaaaac aacaattcct
     1741 ttagacgttc tttattggta ttttttgcct gataggtttg aaaattagcc tttttctctt
     1801 tcgggatata ctcttcgtac tggtactcat gggactgata gtgtcttaat gccatatcgt
     1861 ctccttttct gctccttaaa gaataccata acattgctga atttttatgt catttatatt
     1921 acaataatgt tacaagagaa agattagaaa agtctgaaca ttaaaataca ggtagtcact
     1981 tatcagatat ttctcttttt gatataatta tctttatgga aaaaattatc attacagcaa
     2041 cagctgaaag tattgagcag gtcaaagaac tgcttgtagc aggcgttgac cgcatttatg
     2101 taggtgaagc tgaccatgct cttcgcatcc caacaaattt tacctatgat gaactcaggg
     2161 aaatagcaga gctagttcat agcgctggga aggaattgac ggtcgctgcc aatgccctca
     2221 tgcatcaaga tatgatggac aatatcaaac catttatgga gctaatgagg gaaatcaagg
     2281 ttgattatct ggttgtcgga gatactggaa ttttctatat caataaacgt gatggctaca
     2341 atttcaagct aatctatgat acatctgtct ttgtgacttc cagtcgtcag attaactttt
     2401 ggaaagatca tggagcagta gaggctgttt tggcaagaga gattccatca gaagaactct
     2461 ttgatattgc taagaacttg gaaatgccag cggaaatctt ggtctacggc gcctctgtta
     2521 ttcaccattc taaacgaacc ttgttacaga attattacaa ttttacaaag gctgatgaga
     2581 cagatttatc acgtggacgc ggacttttct tagctgagcc gagcgaccca gatagccact
     2641 attctatctt tgaggacaag cacggtaccc atatctttat caacaatgac attgatatga
     2701 tgaccaagct gtcagaatta gatgaacatg gttatcataa ttggaaactt gatggtatct
     2761 attgccctgg acagaacttt gttgaaattg ttaagctttt tgttcaagca agagatttga
     2821 ttgaaaaagg cgaattcact tatgatcagg ccttcctttt ggatgagcag gtacgcaagc
     2881 tccatccagc tgaacgtggc ttagatacag gcttctacga atttgaccgc aataaggtta
     2941 aataacaata tttatagcac aatttattta ctttaacctg acagagttag ctctgtcact
     3001 tattatgtaa ggtcagagca cgaaaacgcc tttgagcgat tttctaaact ctgcactagg
     3061 tttccacatg gaaaagtatc gtttcatgtg gaaaccgtcg caaatagagg agattttaca
     3121 tgatgtcaaa aacattaaag cgtcccgagg tcttgtcacc tgctgggact ttggagaaat
     3181 tgaaggttgc cattgattat ggtgctgatg cggtctttgt tggcggccag gcctacggtc
     3241 tgcgtagccg agctggtaac tttaccatgg acgagatgcg ggaagggatt gaatatgccc
     3301 atgcccgtgg tgccaaggtc tatgtggctg ccaacatggt cacccacgaa ggtaatgaag
     3361 aaggagcagg agcctggttc cgtgaattgc gtgacatggg cttggatgcc gttatcgtat
     3421 ctgacccggc tcttattgtt atctgtgcta ctgaagcgcc aggccttgaa attcacctgt
     3481 caacccaggc ttcttccacc aactatgaga ccttcgagtt ctggaaggaa ttgggcttga
     3541 ctcgtgtcgt cctggcccgc gaagtgacta tggacgaggt ggctgaaatc cgtaaacgga
     3601 cagacgttga gattgaggcc tttgttcacg gagctatgtg tatttcctat tcaggtcgct
     3661 gcgttctctc taaccacatg agcaaacgcg atgctaaccg cggtggctgc tcccagtctt
     3721 gccgttggaa gtacaatctc tacgacctcc catttggcga agaacgccgt tcgattaagg
     3781 gggaaatccc agaggagttc tccatgtctg cagtggacat gtgtatgatc gaccatattc
     3841 cagatatgat tgaaaatggg gtcgatagcc tgaaaatcga aggccgtatg aaatctgtcc
     3901 actacgtatc aacagtgacc aactgctaca aggctgcggt agatgcctat cttgaaagcc
     3961 cagaaaagtt tgaagcaatc aagcaagacc tgattgatga gatgtggaag gttgcccaac
     4021 gtgaattggc gacaggtttc tactatagcc cgccgagtga aaatgagcag ctctttggag
     4081 cccgccgtaa gattccagaa tacaaattca tcgccgaagt agtggctttt gacaaggaaa
     4141 ccatgactgc aaccattcgt cagcgtaatg ttatcaacga aggtgaccaa gtagagtttt
     4201 acggaccagg tttccgtcat tttgaaacga ctattcagga cttgcatgat tcaaatggag
     4261 aaaagattga ccgcgccaac aagccgatgg aactcttaac tattaagctg gaccgtgaaa
     4321 tctatcctgg tgacatgatt cgtgcctgca aatcaggcct catcaacctc taccaagaag
     4381 atgggcaatc tctaactatt cgtgcataaa cataaaaagg ctgaaatttc agcctcagaa
     4441 cgtagataaa ctcttaaaaa tataaaaaat tgag
//
LOCUS       DASFSW010000142         4467 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.143, whole genome shotgun sequence.
ACCESSION   DASFSW010000142 DASFSW010000000
VERSION     DASFSW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.143"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..262
                     /locus_tag="U1099_001829"
     CDS             <1..262
                     /locus_tag="U1099_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEM4868118.1"
                     /translation="MESEEATPSLSQAQRMKSMSQSGQLDMDMIFSIMTEEKGNQKET
                     LKINTSKLKKYFPKDTTPKQMEETIIRLLERELQRKRSRDSR"
     gene            339..569
                     /locus_tag="U1099_001830"
     CDS             339..569
                     /locus_tag="U1099_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868119.1"
                     /translation="MKEIQYEIVKEIAVLSASDSGYTKEINLISWNGREPKYDIRSFS
                     PNREKCGKGITLNADEAAALLEALQKEVNSGD"
     gene            640..831
                     /locus_tag="U1099_001831"
     CDS             640..831
                     /locus_tag="U1099_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HEM4868120.1"
                     /translation="MGEDRKTTGRRKRIVPKGQVQMIISREYIGTQTVAEAFVPIISE
                     DIRKKIAEGDTFDNEGLSA"
     gene            885..2768
                     /locus_tag="U1099_001832"
     CDS             885..2768
                     /locus_tag="U1099_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM4868121.1"
                     /translation="MAGIRTENKIYEVGIYCRLSKDDGTDNESASIATQKSILTDYVK
                     RQGWHLAKTYVDDGYSGTNFQRPSFQNMIKDIENGLINCVITKDLSRLGRNYLDCGLY
                     LEVFFPEHNVRYIAVNDGVDTLNKSAMDITPFRNILNEMYSADVSVKIKSAYRARFQQ
                     GKFMGTTAPYGYVKDPADHNHLLIDDKVAHVVREIFDLALAGNGIAKIRKHINKQHIL
                     RPAAYAVEQGATGYERYFEDNEENRYIWSENSVRGILRSPIYAGNLAGYKRIAANMKS
                     KKRPSKLPEEWEVIPDTHEGIVTQEEFDTVQQLMTSRRREQNAGGFENIFSGVIKCAD
                     CGYAMRAASANRRKRPDIIDCVQYTCNNYGRYGNVMCTAHSIEARDLFNAVLADINRF
                     ADMAVNDEKAVRAIERRLTETDQSRAKSLEKEKKKLNKRLAELDRLFSSLYEDKVMER
                     ITERNFEMMSGKYQKEQLEIEARLKEVTETLNDSYEKSQGVRDFLSLIRNYQGLKELD
                     ATIINALIDKILVSEREKLADGTVRQEIKIYYKFIGFVGELHITPTKRWTALKPKNCT
                     VCGVEYVPSSGISKYCPACAKRIQREKSNESKRRSRERNRRACIELSAKNDRLILSNA
                     MVD"
     gene            2783..3694
                     /locus_tag="U1099_001833"
     CDS             2783..3694
                     /locus_tag="U1099_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HEM4868122.1"
                     /translation="MHQEVDIYHYITKGSFDNYLWQTQENKLKYITQIMTSKDPVRSA
                     EDIDEQTMTASDFKALATGNPYLKLKMELENELTVLDNQKRAFHRTKDEYRHTISYCE
                     QNLPVLEKRLSQYDRDIAQSLATKSQDFIMRFDNQMMDNRAEAGDYLRKLITYNRSET
                     KEVRTLATFRGFELKMATRSPSEPLPDMVSLTISGSNQYSVSLDLKSDVGTIQRITNA
                     IDHILEDQEKTEEMANNLKDKLAVARVEVEKVFPKEEDYQLVKAKYDILAPLVEQEAE
                     VKEIDAALAKFNETIQPQQDQQLSLDF"
     gene            3764..4063
                     /locus_tag="U1099_001834"
     CDS             3764..4063
                     /locus_tag="U1099_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868123.1"
                     /translation="MTQTVEELRYQLEEWLAQGFTSPEDRANYQALKEQYEDETLDYS
                     FSKREIIGQLEVIITTRENDFPDLDEVTKGEYLDLVEQLDNLDKGQADYYRKQLA"
     gene            4078..4368
                     /locus_tag="U1099_001835"
     CDS             4078..4368
                     /locus_tag="U1099_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868124.1"
                     /translation="MIEQIIQSLFIIAATGLILLVLFQIAKMLGSLFVIGLIGFLAFT
                     EVYGIYLFFTERYLYVEDLATNGILSFTTFYIIFNLLLVFGVVRKVVRSRMA"
BASE COUNT         1464 a          876 c         1102 g         1025 t
ORIGIN      
        1 gatggagagc gaagaagcaa cgccctcact ctcacaggca cagcggatga aaagcatgag
       61 ccagagcggg cagcttgaca tggatatgat attttccatt atgacggagg aaaagggaaa
      121 ccagaaagaa accttgaaaa tcaacacaag caaactgaaa aaatactttc cgaaggacac
      181 aacgccgaag cagatggagg aaaccatcat ccgacttctg gaacgtgagt tgcagagaaa
      241 acggagcagg gacagccgct aaccttcttt tttcgggaag taaatgcagg aatttggaac
      301 agaagaaaga cagaatttta aggagaatga ggtaaggaat gaaagaaatc cagtatgaga
      361 ttgtaaagga aatcgccgta ttgtctgcga gtgacagcgg ctacacaaag gaaatcaatc
      421 ttatttcatg gaacgggaga gagccgaaat atgacatccg cagcttttcc ccgaaccgtg
      481 agaagtgcgg aaagggtatc acgttgaacg ctgatgaagc agcggcactc cttgaagcat
      541 tacagaaaga agtaaacagc ggggattgat ggtatctgat tgacagggcg gggcgtttcc
      601 agacgttctc ctgccctgtc tggaaaggaa gatttaagta tgggcgagga taggaaaaca
      661 actggaagaa gaaagcgtat tgtgccaaaa gggcaagtac aaatgattat cagtcgagaa
      721 tatatcggca cacagaccgt tgcagaagcg tttgtcccga ttatctccga ggatattcgg
      781 aagaagattg ccgagggcga caccttcgac aatgaggggc tgtccgctta gaatgtacgc
      841 aatggaacat gaaaacgtta ggacagatat ggaggtttta cagtatggca ggaataagaa
      901 cggagaacaa gatttatgaa gtcggcattt actgccgctt atcaaaggac gatggcacgg
      961 ataacgagag tgcgagcatt gcgacacaaa aatccatcct cacggattat gtgaaaaggc
     1021 agggatggca tttagcaaaa acgtatgttg acgatggcta ctctggaaca aatttccaaa
     1081 gaccaagttt ccagaatatg attaaggaca ttgaaaacgg gctgataaac tgcgtgatta
     1141 caaaagattt atcccgtctg gggaggaact atcttgattg tgggttatat ctggaagtct
     1201 ttttcccaga gcataacgtg aggtatatag cggtcaatga cggcgtggac accttaaaca
     1261 aatccgctat ggacatcacg cctttccgca acatcctaaa cgaaatgtat tctgccgatg
     1321 tgtcagtcaa gataaaatcg gcgtaccgtg cgaggtttca gcaggggaaa ttcatgggaa
     1381 caacagcccc ttatggctat gtcaaagacc ctgccgacca caaccatctg ctgatagatg
     1441 acaaagttgc ccatgtggta agggagatat tcgaccttgc gttagcgggc aacggcatcg
     1501 ccaaaatccg caagcacatc aacaaacagc atatcctacg ccctgccgct tatgcggtgg
     1561 agcaaggggc aacaggctat gagcggtact ttgaggataa cgaggaaaac cgttatattt
     1621 ggagcgagaa cagcgtgagg ggcattttaa gaagcccgat atatgcgggc aaccttgcag
     1681 gctacaagcg gattgcagcc aacatgaaaa gcaagaaacg cccctctaag ctgcccgaag
     1741 aatgggaagt gataccagac acccatgagg ggatagtcac gcaggaggaa tttgacaccg
     1801 tacagcagct tatgacgagc cgcaggcggg agcagaacgc agggggattt gagaatatct
     1861 tttcgggcgt tatcaagtgt gcggactgcg gctatgcgat gcgggcggcg agtgccaaca
     1921 ggaggaaacg ccccgacatc atcgactgcg tacaatacac ctgcaataat tatggcagat
     1981 atggcaatgt tatgtgtacc gcacacagca ttgaagcgag ggacttattc aatgccgtcc
     2041 ttgccgacat caaccgcttt gcggatatgg cggtcaatga tgaaaaggcg gtcagagcca
     2101 tagaaaggcg gctcacggaa acagaccaga gcagggcgaa atcattagag aaagaaaaga
     2161 agaagctgaa caaacgcctt gcggagcttg acaggctgtt ttcctctctc tatgaggaca
     2221 aggtaatgga gcgtatcacg gagcggaatt ttgagatgat gtcggggaaa taccagaaag
     2281 agcagcttga aattgaagca cggctgaaag aggtaacgga aacgctcaat gacagctatg
     2341 agaaatcgca gggcgtgagg gactttctat ccctaatccg caactatcag gggctgaaag
     2401 agttggacgc aaccatcata aacgcactca tagacaagat acttgtttcg gaacgtgaga
     2461 aacttgcgga cgggacagta aggcaggaaa tcaagattta ctataaattc atcggctttg
     2521 tcggtgaatt acatatcaca cccacaaagc ggtggacggc gttaaagcct aaaaattgta
     2581 cggtgtgcgg tgttgaatac gtccccagct ctggaatatc aaagtattgt cccgcttgtg
     2641 ctaagaggat acagagggag aaatcaaacg agagcaaacg caggagtagg gagaggaacc
     2701 gaagggcatg tattgaactg tccgcaaaaa atgaccgact gatattgtcc aacgcaatgg
     2761 tagattaatt cgtcaaggca atatgcacca agaggtagat atttaccatt acattacgaa
     2821 agggagcttc gacaactacc tctggcaaac tcaggagaat aaactcaaat atatcacgca
     2881 aatcatgacc tctaaagatc ctgtacggtc ggcagaagac attgatgaac aaaccatgac
     2941 tgcttcagac tttaaggctt tagcgacagg taatccttat ctcaaactca agatggaact
     3001 agagaatgag ttgacggtat tggacaatca aaagcgtgcc tttcatcgca ctaaagatga
     3061 gtatcgccat accatttctt actgtgagca gaacctccct gtcttagaga aacgcctcag
     3121 tcaatatgac cgtgatattg cccaatcttt ggcaactaag tcacaagatt ttatcatgcg
     3181 gtttgacaat caaatgatgg ataaccgtgc agaagctgga gactatctac gaaaactcat
     3241 tacctacaac cgttcggaaa ccaaagaagt aagaacgctt gcgaccttta gagggtttga
     3301 gcttaaaatg gcaaccagaa gtcctagtga gcccttgcca gatatggtgt ctttaaccat
     3361 ttcaggtagc aaccagtatt ctgtctcttt agacttgaaa tcagatgtgg gaaccatcca
     3421 acgcattact aatgccattg accatatcct agaggaccaa gaaaaaacag aagaaatggc
     3481 taacaatttg aaagataagc tagcagtcgc aagggttgaa gtagagaagg tctttccaaa
     3541 agaagaagat tatcagctgg ttaaagccaa atatgacatc cttgcccctt tggttgaaca
     3601 agaagctgag gttaaggaaa ttgatgcagc cctagctaag ttcaatgaaa cgatccaacc
     3661 acaacaggac caacaacttt cgcttgattt ttaagaaaaa ggctgacaaa aaataatgaa
     3721 cgcggtaaaa tgaagtcaag aaatcatcaa aggagaattg attatgaccc agacagtaga
     3781 agaattaaga taccaactag aagaatggct agcacaaggt tttacaagcc ctgaagatag
     3841 agctaactat caagccttaa aggaacagta tgaggatgaa acccttgatt acagcttttc
     3901 aaagcgagaa atcattggtc aattagaagt tatcatcacg actcgagaga atgactttcc
     3961 agacttagat gaagtgacta aaggagagta tctagacctg gttgaacaac ttgataatct
     4021 agacaaggga caggcagatt attatcgtaa gcaattagcg tagaaagagg tacagatatg
     4081 attgaacaaa ttattcaaag tcttttcatt atcgcagcaa cagggctgat tttacttgtc
     4141 ttgtttcaga ttgcgaaaat gcttggaagc ttatttgtca ttggtttaat cggattttta
     4201 gctttcacag aagtctatgg aatttacctc ttttttacag aacgatacct ctatgtggaa
     4261 gatttagcca ccaatggtat tttgagcttt accactttct atattatttt taatcttttg
     4321 cttgtctttg gagttgtaag aaaagttgtt agaagtcgga tggcatgatg taatgggatt
     4381 acctaagtta ggtagtcctt ttctttattg tgataacttt tccaagtcca tcttgttatc
     4441 atggtgctat cttatggaaa aaggaga
//
LOCUS       DASFSW010000143         4450 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.144, whole genome shotgun sequence.
ACCESSION   DASFSW010000143 DASFSW010000000
VERSION     DASFSW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.144"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            1..873
                     /locus_tag="U1099_001836"
                     /pseudo
     CDS             1..873
                     /locus_tag="U1099_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            1170..1589
                     /locus_tag="U1099_001837"
     CDS             1170..1589
                     /locus_tag="U1099_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868125.1"
                     /translation="MTYNMTDTTLKIGQLDLDMTQFEVPKKIVILSAKVNNKYNEVNG
                     EQVKTDEVVKVVCNVLDADKVKILKELGISTEDLKAINLEIVGNVDKIATLAQNKSLL
                     NVPIELIKPKVRLAWNMTRSNWAGVKLVCEDIKILGA"
     gene            1594..2610
                     /locus_tag="U1099_001838"
     CDS             1594..2610
                     /locus_tag="U1099_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM4868126.1"
                     /translation="MNTVRIDYFAVTVKNIPPESILTDILLIPLEDFTLNNWGINKYQ
                     RHYACSEIKVYFNEDRPSMGVFIELKGQGCRQYEEFLDGNENNWIALIGRLYRYSVNF
                     TRLDIAHDIFDGSLDVQRVYDYCKKGLCISKAKHFEYHEKSVLENGERVGETVAIGSR
                     GNQQWCIYNKRMEQLSKQELVEYPSWIRAELRCWQDKANIIAEQLFLKRPLSSIYFEA
                     INGHYRFVKPNATDTNRWRRKKVKWWLDYLKTENQTVLSVERTKPTLRQSEAWTEKQV
                     AKTLAKVYTAKYQAYDVQKAEDYIQGLLKEGLSRLTDNDEKDIEQYIREQNSSPLWGQ
                     KKTT"
     gene            2671..2877
                     /locus_tag="U1099_001839"
     CDS             2671..2877
                     /locus_tag="U1099_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEM4868127.1"
                     /translation="MIATVNKNDLVALGFSEGTSKRIIRQGKELLIARGFRVYQNKRV
                     GTIPASIATELLGFDVSLGAHHDS"
     gene            2867..4000
                     /locus_tag="U1099_001840"
     CDS             2867..4000
                     /locus_tag="U1099_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM4868128.1"
                     /translation="MTVKKAKNGTWTVDISDGFHPVTKKRIRIIRKGFKTKKEALELE
                     QYIRVVELKEKRFDFLVTTDMLFDILEEEDRQNNRKISYISTQRNNYERHIKPYFINT
                     NLNKLSYEHIFEFREYLKIKPKKQNDSSTLSHNTVNKIMILLKKIFDTGVRKSLIDKN
                     PVENIRKLPISKPTINFWNVEEFNEFRKLITSDEISYDLFFTIAFFTGMRLGEIVALT
                     WYDINLITNSIHITKTAYFVNGTNHINSTKTRAGTRRITINHKLARMLADWKEKQKDL
                     LKEFTTDTENLQVIQSTPLPMTKNMIDKKFKQILARNDYLKKIRIHDLRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKTLANKLDDIF"
     gene            complement(4087..4170)
                     /locus_tag="U1099_001841"
     tRNA            complement(4087..4170)
                     /locus_tag="U1099_001841"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4134..4136),aa:Leu,seq:caa)
     gene            complement(4182..4253)
                     /locus_tag="U1099_001842"
     tRNA            complement(4182..4253)
                     /locus_tag="U1099_001842"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4219..4221),aa:Gln,seq:ttg)
     gene            complement(4265..4337)
                     /locus_tag="U1099_001843"
     tRNA            complement(4265..4337)
                     /locus_tag="U1099_001843"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4302..4304),aa:His,seq:gtg)
     gene            complement(4345..4415)
                     /locus_tag="U1099_001844"
     tRNA            complement(4345..4415)
                     /locus_tag="U1099_001844"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4381..4383),aa:Trp,seq:cca)
BASE COUNT         1559 a          746 c          914 g         1231 t
ORIGIN      
        1 ctcggtcaca tagaaaaaat tgaacagtat gttttcaata gccatacatt gactaaagaa
       61 ttttttgaag ataatcctag tccaataatc aagctatcaa atactcagca atttagtcta
      121 aaatcaaata ctaatctcgg gatttacggt cgaactggaa cagggaagac aatagctcta
      181 caatggtact tatttaatgc actagctaaa ggttgtggca ctactgacaa tacttatttg
      241 ggaattgttg atggtaaagg agctgattta tttgcccttg gtaaacttct tcaagaagaa
      301 ttaggggagc aaatagcagt aggctcttcg cctcaaatgt tagcaaaatt atcaagagaa
      361 tttgttcaga ttatggggga acgttttgaa attattgagc gaaacagcac gctaaatgca
      421 gatgcttatg atttaggaat gacccccaat tttttatttg ttgatgaact tgcttccatt
      481 cgtgatagct gtggttctag taaacagggc aaagagctgt ggaatgaaat tctccaaaac
      541 ttaggcttaa ttgctcgtaa ggggaggcag gcggggtgcc acttagttct gtcaactcaa
      601 gaccctaatt cctaaaatat aaatgtagag ctaagaaatc aaatttcggc aattttatac
      661 cttggaaatc cgggtaatga ccgattaaaa atggctttta gtatgtgtga acttgaaagc
      721 gtacctacta tatcagatag aaaaggagaa gcgctttttt atgctgacgg tataggttca
      781 gttgaaccag taataacaat cgttccgttc gttgatgtta aaactaaaca acaattttta
      841 caggtagtta gaaatatact gccaaactac taatggcggt ctttttccct agagaaatag
      901 ggacgattgc ctagagggca aaatcgagcg gagcgttcct cttttcgcta gggagaaaga
      961 gccaaccgac tgctttgcag gaggtcagaa aaataccccc attactaata gggggtgcac
     1021 acatacatga aatgtactct aaacccttga tacgtaaggg gttacggctt gttggttata
     1081 ccgaagtcat tgaaaccctt gtgggagaag ggcttgagtc gcttttcgtg taggatacga
     1141 ctttttgaat agaaaatgga gtaattatta tgacttacaa tatgacagat actaccctta
     1201 agattgggca acttgatttg gatatgacac agtttgaagt tccgaaaaaa attgttatcc
     1261 tatctgctaa agtgaataac aagtataatg aggttaatgg tgagcaagta aagactgatg
     1321 aggttgtgaa agttgtttgt aatgtacttg atgctgataa ggttaaaata ctaaaagaat
     1381 tgggaatttc aactgaagac ttaaaagcta tcaacttaga aattgtaggt aatgtagata
     1441 agattgctac acttgctcaa aataaatcgc ttttgaatgt tccgattgag ttgattaagc
     1501 ccaaagttcg tttagcttgg aacatgacaa gaagtaactg ggcaggtgtt aaactggtat
     1561 gtgaggacat taaaattcta ggagcataac cctatgaata cagtcagaat tgattatttt
     1621 gcagtaaccg ttaaaaatat tcctccagaa agtattttaa ctgatattct tttaattcct
     1681 ttagaagatt tcacattaaa taattgggga ataaacaagt accagcgaca ttatgcttgt
     1741 tctgagatta aagtgtactt taatgaggat agaccttcaa tgggggtttt tatcgaatta
     1801 aaagggcaag gttgtcgcca atatgaagaa tttttggacg gtaacgaaaa taattggata
     1861 gctttgatag gtcggttgta tcggtattcg gttaatttta ctcgcttaga tattgctcac
     1921 gacatatttg acggtagttt agatgttcag cgagtatatg actattgcaa gaaaggttta
     1981 tgtatctcga aagcaaagca ttttgagtat catgagaaat ctgtcttaga aaatggagaa
     2041 cgagttggag agacggttgc tataggttcc agaggcaatc aacaatggtg tatctacaat
     2101 aaacgtatgg aacaacttag caagcaagag cttgtagaat atccctcatg gattcgtgcg
     2161 gaacttagat gttggcagga taaggcgaat attattgcag agcaattgtt tctaaagcgt
     2221 cctctgtcat ctatctattt tgaagctatc aatggtcact atcgttttgt gaaaccaaat
     2281 gcgacagata ctaatcgttg gagaagaaaa aaggtcaagt ggtggctaga ctatctaaag
     2341 accgaaaatc agactgtttt gagcgttgag agaactaagc ctacattacg gcagtcagag
     2401 gcttggacag agaaacaagt tgcgaagaca ttggcaaaag tctatacagc aaaatatcaa
     2461 gcctatgatg ttcaaaaggc agaggactac attcaaggtc tactaaaaga gggcttgtca
     2521 aggctaactg ataatgatga aaaggatata gagcaatata ttcgagagca gaatagttct
     2581 cccttatggg gacaaaaaaa gacgacttga aaacaagtcg cctctaaaaa ttaactaact
     2641 agattataac aaaagaaagg aaagtgtcca atgatagcaa ccgtaaataa gaatgaccta
     2701 gttgcccttg gtttctctga aggaacttct aaacgtatca tcagacaggg gaaagagctt
     2761 ctaatagcaa gaggatttag ggtttatcaa aataagcgtg tcggaactat ccctgcttct
     2821 atcgctactg aattacttgg ttttgacgtt tctttaggtg ctcatcatga cagttaagaa
     2881 agcaaaaaat ggaacttgga ctgttgatat ttctgacggc ttccacccag ttaccaaaaa
     2941 gcggatacga atcattcgta aaggtttcaa gacaaagaaa gaagctttag agttagaaca
     3001 atatatccgt gttgttgagt taaaagaaaa acgttttgat tttttggtaa caaccgatat
     3061 gctcttcgat atacttgagg aagaagaccg acaaaataac agaaaaatca gttatatcag
     3121 cacacaaaga aacaactatg agcgacatat caaaccttac ttcataaata caaacttaaa
     3181 taaattatct tatgagcaca tttttgaatt tcgagagtat ctcaaaataa agcccaaaaa
     3241 gcaaaacgat agctctacgt taagtcataa tacagtcaat aaaataatga tactcctcaa
     3301 aaagattttt gatactggtg taagaaagtc attgatagat aaaaatccag ttgaaaatat
     3361 aagaaaattg cctattagta agccaactat aaatttttgg aatgtagaag aatttaacga
     3421 atttagaaaa cttattacaa gtgatgaaat aagttacgac ctattcttta cgattgcctt
     3481 ttttactggc atgcgattgg gcgagatagt tgcgttaact tggtacgata taaacttaat
     3541 cactaatagc attcacatca caaaaacagc atattttgtt aacggtacaa atcatatcaa
     3601 tagtacaaaa actagagcag gtaccagacg tattacaatc aatcataaat tagcaagaat
     3661 gcttgcagac tggaaagaaa aacaaaaaga cttacttaaa gaatttacaa cagatacaga
     3721 aaaccttcaa gtgattcaaa gcacaccact accaatgaca aaaaatatga ttgataaaaa
     3781 atttaagcag atactggcta ggaacgacta tctaaaaaag atacgaatac acgatttaag
     3841 gcactctcac gcgtctttac ttatcaatca aggggaagat tatctagtcg taaaagaacg
     3901 tcttggacac gcctctatca cgactacaat agatacatat tcccacttat accctagtaa
     3961 acaaaagaca ttggcgaaca aattggacga tattttttag aaaatggaat tttttggcaa
     4021 cttgaaaaag agcaatagaa aagataaaac aaaagacaag gcttagaaac cttgtcttta
     4081 taactatacc ggcggccggg gtcgaaccgg cacgtccttg cggacactgg attttgagtc
     4141 cagcgcgtct gccaattccg ccacgccggc atatagttga actggggtag ctggattcga
     4201 accaacgcat gagggagtca aagtcccttg ccttaccgct tggcgatacc ccaatattaa
     4261 aataggcgag tgaggggaat cgaacccccg aatgtcagag ccacaatctg atgtgttaac
     4321 cacttcacca cacccgccat ataacacggg cagtaggaat tgaacccaca ctgaaggttt
     4381 tggagacctt agttctacct ttaaactatg cccgtaaagg tatggagaga gagggattcg
     4441 aacccccgaa
//
LOCUS       DASFSW010000144         4342 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.145, whole genome shotgun sequence.
ACCESSION   DASFSW010000144 DASFSW010000000
VERSION     DASFSW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.145"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(89..1303)
                     /locus_tag="U1099_001845"
     CDS             complement(89..1303)
                     /locus_tag="U1099_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM4868129.1"
                     /translation="MKQFNKSTKLDDVAYDIRGPVLEEAMRMRANGEQILRLNTGNPA
                     EFGFTAPDEVIRDLIHNARKSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIEDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGKAVHYVCDEAAEWYPDLAD
                     MESKVTSRTKAIVLINPNNPTGALYPKEILKGIIDIARRHELIIFSDEIYDRMVFDGA
                     VHIPIATLAPDLFVVTMNGLSKSHRICGFRVGWMVLSGPKKHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGYQSVDELLMPGGRLYEQREFITKAINDIPGLSAVKPKAGLYV
                     FPKIDREMYRVEDDEQFVLDFLKQEKVLLVHGRGFNWKDPDHFRIVYLPRVDELAEIQ
                     EKMSRFLRQYRR"
     gene            1458..1910
                     /locus_tag="U1099_001846"
     CDS             1458..1910
                     /locus_tag="U1099_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM4868130.1"
                     /translation="MTQSYKTILVAVDGSKGAELALHKAIHVALRNQARLIIAHVIDT
                     RALHNVVAFDASVYESLEREAELLLEEYKQEALNAGLTDVQICIEFGNPKTLLAIDIP
                     KETGADLMLLGATGLNAFERLLIGSSSEYIMRHATIDLLIVRDGEKSL"
     gene            complement(1926..2444)
                     /locus_tag="U1099_001847"
     CDS             complement(1926..2444)
                     /locus_tag="U1099_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4868131.1"
                     /translation="MFQSKDDNFNRVKDFHFLMDGETQELPSVYDGQTALHRAGFKVE
                     ELVEFLHAASESEVEFHDFIQQLHRDLDTAAAKVSGKRGFGVSMQDQVDALLDILYFT
                     YGSFVLMGVDPEPIFQIVHTANMRKTFPDGKAHFDPITHKILKPDDWEERFAPEEKIQ
                     EELKRQMKRLDS"
     gene            complement(2446..3291)
                     /locus_tag="U1099_001848"
     CDS             complement(2446..3291)
                     /locus_tag="U1099_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM4868132.1"
                     /translation="MTIKAVFFDIDGTLLTDNRMVSSSTILAINALKEKGILVGLATG
                     RDPRFVLQYMASLGLDLAIAYNGQYIFSREEVIYSQSLEPKQIEQIMEYAQTHHKDLS
                     FGTAKGIFGSKIMSAGTGNFAYRVTRMIPESWAGIINFIFNRLVRWISPQQETNLKGF
                     LFQPIYQLMLLTTERETQSLEMLFPNLSFTRSSPYATDIISKGNSKLSGIVKVADRYG
                     FELDEVMVFGDSNNDFEMLNEIQHSVAMGNGTKKVKQAASFVTDTNNRDGIYKALIHF
                     GVIEG"
     gene            3361..4320
                     /locus_tag="U1099_001849"
     CDS             3361..4320
                     /locus_tag="U1099_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEM4868133.1"
                     /translation="MKKILVLHTGGTISMQANHQGEVASSKINPMTQIDSPLEEIQVT
                     SLDFLNVPSPHIRLEHMMTLYQKIKEEQMNFDGFVITHGTDTLEETAYFLDTMAIPEK
                     PIVLTGAMRSSNELGSDGIYNYRTALRVAADDKSADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGLVTKREILYFKTAEPRVRFDLSTVTGTVPIIKAYADMDSILLD
                     SLTASSISGLVIEALGAGNLPPTVLPAIKKLLDQKIPIILVSRCFNGIAEPVYAYQGG
                     GIQLEQDGILFVKELNAQKARLKLLIALNAGLEEQSLADYIQG"
BASE COUNT         1389 a         1033 c          699 g         1221 t
ORIGIN      
        1 actgtttcag ctcaacaact agaaataaag acttgttgac gaactcttct tatgaatggt
       61 tgagttcttt cccactccca aaattccatt atctacgata ttgccgcaag aatcgtgaca
      121 ttttttcttg aatttctgcc aactcatcaa cacgtggtaa atacacaata cggaaatgat
      181 ctggatcctt ccagttaaaa cctcgaccat ggaccaaaag tactttttct tgcttgagga
      241 aatccaatac aaattgttca tcatcctcaa ctctgtacat ctcacgatca atttttggga
      301 atacatacag accagcctta ggttttacag cagaaagtcc cggaatatca ttaatcgcct
      361 tggtaatgaa ctccctttgt tcatataagc gtccgccagg catcaagagc tcatcaaccg
      421 attggtaacc tcccaaagaa gtttgaacaa cctgttgagc caaaacgttt gaacacaaac
      481 gcatattgga caacatattc aagccttcaa tgtaaccttt tacatgcttt ttcggaccag
      541 ataagaccat ccaacctact cggaaaccac aaatacgatg tgatttagac agtccattca
      601 tcgtcacaac aaataaatct ggagccagtg tcgcaatagg gatatgaaca gctccatcaa
      661 agaccatacg atcataaatc tcatcagaga aaatgatcaa ttcatgtcta cgagcaatat
      721 caatgattcc ttttaaaatt tctttcggat agagagcacc agttggatta tttggattga
      781 taaggacaat tgccttggtt cgagaagtca ccttggactc catatctgcc aaatctggat
      841 accattcagc tgcttcatca caaacataat gaacagcttt accaccagcc aaactaactg
      901 cggcagtcca gagaggatag tctggcattg gcaccaatac ctcatcacca ttatctagca
      961 aaccttgcat agacataaca atcagctcac tcacgccgtt tccaagataa atatcttcaa
     1021 tgtcaacatt tggaaatttc tttagctgac aatactgcat gatagcctta cgtgcagaaa
     1081 aaattccctt actgttcgaa tatccctctg atttacgggc gttatgaatc aagtcacgaa
     1141 taacttcatc tggagcagta aaaccaaatt ctgcaggatt ccccgtattc aaacgtaaaa
     1201 tctgctctcc attggcacgc atgcgcatgg cctcttccaa aacaggccca cgaatatcat
     1261 atgctacatc atccaatttt gttgacttgt taaattgctt catgtcgtca cctcgacttt
     1321 ctatcttttc tattgtactg taaaagctaa aaaagtacaa gaatccttta cattttctct
     1381 caaaaggctt ataataaaaa tgtaaggaac tactctacct atttcgggac aaagctcttc
     1441 tgaaaggagt gattattatg acacaatctt acaaaacaat actcgtcgct gtagacggct
     1501 ctaaaggagc agaattagct cttcataaag ccattcatgt cgcactacgc aaccaagcac
     1561 gtctgattat cgcccatgtg attgacaccc gtgctctcca caatgttgtt gcatttgacg
     1621 cctctgtcta tgaatcttta gaaagagagg cggagctcct cctcgaagaa tacaaacaag
     1681 aagctctcaa cgccggtctg accgatgtac agatttgcat tgaattcggt aaccctaaaa
     1741 ccctactggc tatcgatatt ccaaaagaaa ccggtgctga tcttatgtta cttggagcaa
     1801 ctggtctaaa cgcctttgaa cgtctcctga tcggatcctc atccgaatat atcatgcgcc
     1861 atgcaactat tgatttactc attgttcgcg atggagaaaa aagtttataa accaaaagac
     1921 ttgatttacg aatcaagtct tttcatctgt cttttcagct cttcctgaat cttttcctcc
     1981 ggcgcaaacc gttcttccca atcatcaggt tttaaaattt tatgggtaat tggatcaaaa
     2041 tgggccttcc catctggaaa agttttcctc atatttgcag tatggactat ctgaaaaatt
     2101 ggttctggat caacccccat caaaacaaac gaaccatatg taaaataaag aatatccagc
     2161 aaagcatcta cctgatcctg catcgaaacc ccgaaacctc tcttgccaga taccttagca
     2221 gcagcagtat ctaaatcccg atgaagttgt tgaataaaat catgaaactc aacttcagat
     2281 tctgaagctg catgtagaaa ttctaccaac tcctccactt taaaaccagc gcgatgcaaa
     2341 gccgtctgtc catcatacac actgggaagt tcttgtgttt caccatccat taaaaaatga
     2401 aaatccttta ccctattaaa attatcgtcc ttgctctgaa acatcttatc cctcaatcac
     2461 accaaaatga attaatgcct tataaatccc atcacgatta tttgtatccg taacaaagga
     2521 agcagcttgt tttacctttt tcgtcccatt tcccatcgct accgagtgct gaatttcatt
     2581 caacatttca aaatcattat tagaatcccc gaataccata acttcatcta actcaaaacc
     2641 atatcgatct gcaaccttta caatacccga taattttgaa tttcccttac tgataatatc
     2701 agtcgcatag ggactcgaac gcgtaaaaga caagttcgga aagagcatct ccaatgattg
     2761 tgtctccctt tccgtcgtca gcaacatcag ctgatagatt ggttgaaaga gaaaaccttt
     2821 caaattcgtt tcctgttgag gactaatcca acgtaccaag cgattgaaaa taaaatttat
     2881 gattccagcc caggactccg gaatcatacg cgtcacacga taggcaaaat ttcctgttcc
     2941 agcactcata atcttactac caaatatgcc tttagcagtt ccaaaagata aatccttatg
     3001 gtgagtttgc gcatactcca taatttgttc aatttgcttt ggctctaaac tctgactata
     3061 aataacttct tctcttgaaa aaatatattg accattataa gcaatggcca aatctagccc
     3121 taaactagcc atatactgca aaacaaaacg agggtcacgt cctgtagcta gaccaactaa
     3181 aatccccttc tctttcaaag cattaatggc gagaatagtt gaactactaa ccatacgatt
     3241 gtcggtcaat aaggtaccat caatatcgaa aaatacagcc tttatcgtca accaactcac
     3301 ctactttcta agaattatgt tacaattagt atatcacact ttgaaagaaa ctgaaaattt
     3361 atgaaaaaaa ttctagtact gcatacaggt ggaaccattt ctatgcaagc caatcatcag
     3421 ggtgaagttg catcgagtaa aatcaatcca atgacccaaa ttgatagccc actagaagaa
     3481 attcaagtga catccttgga ttttctcaat gttccaagtc cacacattcg cttagaacac
     3541 atgatgactc tataccaaaa aattaaagaa gaacaaatga acttcgatgg ttttgtcatt
     3601 acacatggca cagatactct agaagaaact gcctatttcc ttgatacgat ggctattcca
     3661 gaaaaaccaa ttgtattgac cggagccatg cgttcctcaa atgaactagg tagcgacgga
     3721 atttataact accgaacagc cctacgtgtg gctgctgatg ataaatccgc agataaaggt
     3781 gttctagtgg tcatgaacga tgaaattcat gctgctaaat atgttacaaa aacgcatacc
     3841 accaatgtct caacctttca aacaccaacc catggaccac tgggattagt caccaaacga
     3901 gagattctct attttaaaac tgctgaacca cgtgttcgat ttgacttatc aacagtcaca
     3961 ggaacggtcc caatcataaa agcttatgca gatatggact ccattctcct tgattctctc
     4021 actgcaagct caatttccgg tttagtcatt gaagcactcg gcgccggaaa tcttccgcct
     4081 accgtccttc cagccattaa gaaactcctt gatcaaaaga tacccattat tctggtatct
     4141 cgctgtttta acggaattgc tgagcctgtc tatgcctacc aaggaggtgg catccaactg
     4201 gagcaagacg gcattctatt tgttaaagaa ttgaatgctc aaaaagcccg actcaaactc
     4261 cttatcgctc tcaatgcagg cttggaagag caaagcttgg cagattacat ccaaggttaa
     4321 tcgctgacga actatacaat at
//
LOCUS       DASFSW010000145         4332 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.146, whole genome shotgun sequence.
ACCESSION   DASFSW010000145 DASFSW010000000
VERSION     DASFSW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.146"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(173..637)
                     /locus_tag="U1099_001850"
     CDS             complement(173..637)
                     /locus_tag="U1099_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868134.1"
                     /translation="MLGVHLNKVSAQHLLGIMICEKECSIIKIMKYNVKISQKIKEIV
                     GLETSATDILIHEENLEKHMLKSRHRKMIKYIPDVATILDSPDFVGQNKNVKTESFEV
                     IKVLADNVLVAVKLDKKNDYFFVASVYDITDSKLNHMKRNGRIKAFDKIEDK"
     gene            complement(615..1352)
                     /locus_tag="U1099_001851"
     CDS             complement(615..1352)
                     /locus_tag="U1099_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PlySs2 family phage lysin"
                     /protein_id="HEM4868135.1"
                     /translation="MTTVNEALNNVRAQVGSGISVGNGECYALASWYERMISPDATVG
                     LGAGVGWVSGAIGDTISAKNIGSSYNWQANGWTVSTSGPFKAGQIVTLGATPGNPYGH
                     VVIVEAVDGDRLTILEQNYGGKRYPVRNYYSATSYRQQVVHHITPPGTITQSAPNLAG
                     SRSYRETGTMTVTVDALNVRRAPNTSGEIVAVYKRGESFDYDTVIIDVNGYVWVSYIG
                     GSGKRNYVATGATKDGKRFGNAWGTFK"
     gene            complement(1449..1835)
                     /locus_tag="U1099_001852"
     CDS             complement(1449..1835)
                     /locus_tag="U1099_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEM4868136.1"
                     /translation="MFEFLRELIATEDGLVLFLLGLIVTMEIVDFLSGTFAAMINPDI
                     EYKSKIGINGLIRKMMGIILLTVLIPMSVLLPEQTGVAFLYTIYVGYLILTFKSLIEN
                     YGKAKGDTSIFENVTAAFEKLIGKGK"
     gene            complement(1881..2225)
                     /locus_tag="U1099_001853"
     CDS             complement(1881..2225)
                     /locus_tag="U1099_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868137.1"
                     /translation="MTQTTNNTLLNLEETTQPFDLATALRYMKEHGEFIRCKSATQDF
                     YMYRDVQKRPAIVNGRRKFVDVETIWAFNQWGSTTTTINIADMLNEEYWIMKFDEHGN
                     PDWSDPTVGAEV"
     gene            complement(2228..2443)
                     /locus_tag="U1099_001854"
     CDS             complement(2228..2443)
                     /locus_tag="U1099_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868138.1"
                     /translation="MNQELINQALRLTNNDLMTKLSEEMTTKNLLAVQLTEAQQTIAN
                     LRAEITELTKQLDEATKPEEIIEQKGE"
     gene            complement(2443..>4332)
                     /locus_tag="U1099_001855"
     CDS             complement(2443..>4332)
                     /locus_tag="U1099_001855"
                     /inference="COORDINATES: protein motif:HMM:NF019517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868139.1"
                     /translation="QTDYNTLTGRVDSAETAITQNATEISKRLTKTQVDKAITDKGYQ
                     TKSDVDSNITGRGYITNSALQPYALATTVQNLVRETADSFSRTISETKALIPTSVGGR
                     NLIRGSSEMIIGSGRWQDGTFRKSGTGNIKTINISNPPVPNVTKGIEVEIASATECGI
                     AQDTLFLPKGTYTFTVWVKGPTGATGRIDTFNGSGSKSKVFKLTGGWDKVTVTNTSTV
                     DETKNVGYVYLQNSPGYPAKMHLTAPKLEAGTIPTDYTLAIEDLATVTALHDVKDTVS
                     SHTRTIGAVGETGSILDNVSKVTQTAAGLVQEVSGDNGLKTQVSQLAGSYAIKNLTSS
                     GKVLNQLNLNKDGSVKIDGSLVQITGRTYIQDGVITSAKIAGLDAGKVTTGYLASARI
                     KANSIDGSKIAFDEAFFNRLVANQAYLKKLFAKDAFLTAVQAVSLSASQIIGGLMRAT
                     NGAMEIDFNNSAIDMFKDGAIRFHSGSNAIYRQSTDGIHTAFVHFDTTSKGGIYASLG
                     STSSRDGINSKSSGRFAGIRVARTSERSDSHVASQDAVELYGDTIFFGHGFEGGGFSM
                     HTTLTGGRTISLNKMHDALINLCRCWIHLRNEGFNLNSSAFRKSLDTEIHNAQQLYNT
                     IERIT"
BASE COUNT         1106 a         1009 c          882 g         1335 t
ORIGIN      
        1 cggtctgttg ctaccgacat ttgtgtcgac aacaaaaaaa gcccccagcg tttgctgagg
       61 ggtttttgaa atacaatgat agcggttggg cggcgtatcc aacatctcag gtactctctc
      121 gagtgcgtca ggagcctttc tgacctcgtt atcattcatt gtatttagtt tactacttat
      181 cttctatttt gtcaaatgct ttgatgcgac cgtttctctt catgtgattt aatttggaat
      241 ctgtgatgtc ataaaccgaa gccacaaaga aatagtcgtt tttcttatct agtttgacag
      301 cgaccagaac attatctgca aggactttga taacttcaaa actctctgtc ttcacattct
      361 tattttgacc aacaaaatcc ggcgaatcca aaatagtagc aacatctggg atatatttga
      421 tcatctttcg atgtctgctt tttaacatat gtttttcaag attttcttcg tggatcagga
      481 tatcggttgc agaagtttct aaccctacta tttccttgat tttttgactg atttttacat
      541 tgtatttcat gatttttatt atactacatt ccttttcaca aatcataatc cccagcaaat
      601 gctgggctga aactttattt aaatgtaccc caagcattgc cgaaacgctt accgtctttg
      661 gtagcgcccg tcgcaacgta gttacgtttg ccgctgccgc ctatgtaaga cacccagaca
      721 tagccattga catcgatgat gacagtatca tagtcaaatg attcaccacg cttgtatact
      781 gctacaatct cgcctgaagt atttggcgcc ctgcgaacat tgagagcatc gaccgtgaca
      841 gtcatagtgc ccgtctcgcg ataggaacga gagcctgcaa ggttgggtgc tgactgtgta
      901 atcgttccag gcggtgtgat gtggtgcacg acttgttgac gatagcttgt agcgctgtaa
      961 taattacgga cgggataacg tttcccgccg tagttttgct ccaaaatagt caatctatcg
     1021 ccgtccactg cttcgacgat taccacatgt ccgtaagggt ttcctggtgt tgccccaagc
     1081 gtcacaatct gacctgcttt aaatggacca gatgtggaaa ctgtccagcc gttagcttgc
     1141 cagttgtatg atgagccgat gtttttggca gagattgtat cgccgattgc accgctgacc
     1201 cagcccacac cagcgccaag tccgacagtt gcatccggac taatcatgcg ctcgtaccaa
     1261 ctagccaaag cgtagcattc gccgttacct actgatatgc cagacccgac ttgggcacgg
     1321 acgttattta aggcttcatt tactgttgtc atatttatct tccttttcaa aatagaaagg
     1381 agctagcacc cacaaaatag gcgtcatagc tcccaaaaca ataataatca agattgcttt
     1441 gattggtttc atttgccttt accaatcaac ttttcaaaag ctgctgtcac gttttcaaat
     1501 attgaggtgt cgcccttggc tttgccgtag ttctcgatca agctcttaaa ggtcaagatg
     1561 agatagccaa cgtagattgt gtagagaaat gccacacccg tctgttcagg caacaagacc
     1621 gacattggta tcaacacggt aagcaaaata attcccatca ttttacgaat caggccatta
     1681 ataccgattt tagacttgta ttcgatgtct gggttaatca tagcagcgaa tgtacctgat
     1741 aaaaaatcga caatttccat ggtaacaata agcccaagca aaaagaggac tagaccgtcc
     1801 tctgttgcga ttagctcgcg taaaaattca aacattcctg caggatgtgg tggcgtttta
     1861 gataacatca taaccatgta ttacacctcc gccccaactg tcgggtctga ccagtctggg
     1921 ttgccatgtt cgtcaaattt cataatccaa tactcttcgt tgagcatatc agcaatgttg
     1981 atagtcgtcg tagtactacc ccactggtta aatgcccaaa tcgtttccac gtcaacaaac
     2041 ttgcgacgac cattcacaat tgcaggacgt ttctgcacgt cgcgatacat ataaaagtct
     2101 tgtgtcgctg acttgcagcg gataaattca ccatgttctt tcatgtagcg gagtgcagtc
     2161 gcaaggtcaa atggttgtgt tgtttcttcc aaattaagca atgtgttgtt agtagtttga
     2221 gtcatgatta ttctcctttt tgttcaatga tttcttctgg tttagtagct tcgtccagtt
     2281 gctttgttaa ctcagtaatt tctgcccgca aattggcaat cgtctgctgc gcttcggtta
     2341 gttgcacagc gagcaagttc ttggtcgtca tttcctctga caatttagtc atgaggtcat
     2401 tgttagtcaa gcgtagcgct tggttgatta gttcttggtt cattatgtaa tcctttcgat
     2461 tgtattataa agttgttgtg cgttatgaat ttctgtatct aacgacttcc tgaatgctga
     2521 actattcaaa ttaaaacctt cgtttcgcaa gtgtatccag cagcgacaaa ggttaatgag
     2581 tgcgtcatgc atcttgttta gactaatcgt tcggccgccc gtcagagttg tatgcatcga
     2641 gaaacctccg ccttcgaaac catgaccgaa gaaaattgta tctccgtaca gttcaacagc
     2701 atcctgcgac gcaacatgag aatctgaacg ctcagaggtc cgagccactc ggataccggc
     2761 aaaacgacct gaagacttac tgttaatacc atcccttgat gatgtcgaac caagagaagc
     2821 atatatcccc cccttggatg ttgtgtcgaa atgcacaaac gctgtatgga tgccatctgt
     2881 actttgacga tagatagcat tagagcctga gtgaaatctt atcgcgccat ctttaaacat
     2941 atcaatagcc gaattattaa agtcaatctc catagctcca ttggttgccc tcatcaatcc
     3001 acctatgatt tggctcgctg acaaacttac agcttgcact gctgtcaaaa atgcatcctt
     3061 ggcaaataac ttcttcaggt aggcttggtt cgcgactaac ctgttgaaga aagcttcatc
     3121 gaatgcaatt tttgacccat cgatactatt cgctttaatc cgtgcagatg ctaaataacc
     3181 cgtcgtaacc ttaccagcat ctaagcctgc aatcttagcg gatgtaatca caccgtcttg
     3241 tatgtaagtc ctaccagtaa tctgtacgag actgccgtca atcttgaccg aaccgtcttt
     3301 atttaggttg agctggttaa gtaccttgcc tgagctggtc aaatttttaa tcgcataaga
     3361 cccagccaat tgactgactt gtgtcttgag tccattatca ccagatactt cctgcaccaa
     3421 acccgctgca gtctgagtaa ctttgctgac gttgtccaaa atactgcctg tctcaccgac
     3481 agcgccgata gtacgggtgt ggcttgatac cgtatctttg acatcgtgta gggcagtgac
     3541 agtggccaag tcttcaatgg ctagtgtata atccgtcgga atggttcctg cctcaagttt
     3601 tggagctgtt aaatgcattt tcgcagggta accgggagaa ttttgtagat atacataacc
     3661 gacattttta gtttcgtcta ctgtcgatgt attagtgact gttactttat cccagccgcc
     3721 tgtcaattta aacacttttg attttgaccc gctaccatta aacgtatcaa ttcgtccagt
     3781 ggctccagtt gggcccttga cccaaacagt gaatgtgtag gtgccctttg gtaaaaatag
     3841 tgtgtcttgg gcaatcccac attcggtagc tgacgcaatt tcaacttcta ttcctttagt
     3901 cacatttggg acaggcggat tactgatgtt gattgtctta atattacctg taccagattt
     3961 tcgaaatgtg ccatcttgcc aacgaccact gcctataatc atttcagacg agcctctaat
     4021 cagatttcgt ccacctaccg atgtcggaat cagcgcttta gtctcactta tcgtccggct
     4081 aaaactatcc gcagtctctc tgacgaggtt ttggactgtt gtagccaagg cataaggttg
     4141 caatgcacta ttagtaatat agccacgacc tgtgatgttg ctgtcaacat cagactttgt
     4201 ctgatagcct ttatctgtga tagctttatc aacctgcgtc tttgtcaatc gcttgctaat
     4261 ctctgtcgca ttttgagtaa tcgctgtttc agcgctatcg actcggccag tcaacgtatt
     4321 gtagtccgtt tg
//
LOCUS       DASFSW010000146         4237 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.147, whole genome shotgun sequence.
ACCESSION   DASFSW010000146 DASFSW010000000
VERSION     DASFSW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.147"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..423
                     /locus_tag="U1099_001856"
     CDS             <1..423
                     /locus_tag="U1099_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868140.1"
                     /translation="GDQLQDIWIDSLNKDELEEEFGSYKVYYDGNLIIENGASYTSEG
                     ATKNQIISMKETIVTELDTNKEFKKYTFKKYYIPDEGTDPTEIKIIEDGFAIETVFEQ
                     KDLIPNIAFGPTLYTVRAEFIKENNKLVLRKGSMEVYK"
     gene            420..851
                     /locus_tag="U1099_001857"
     CDS             420..851
                     /locus_tag="U1099_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868141.1"
                     /translation="MKSLTQTFTKKQKLVVVIVVILSVAIGFWKWRDTFFNTYYPDDT
                     SIQGQLFPNVANTKIETVENVLPFSDVKYDKIIIRIGSLSSEYDDSNQSSKANNIINR
                     KTLDENFIQWLIEAFDEEEAKKISDHIEVYYRGEIISEISY"
     gene            1027..3678
                     /locus_tag="U1099_001858"
     CDS             1027..3678
                     /locus_tag="U1099_001858"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEM4868142.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     VTRYDLGREKFLDKVWEWKDEYAATIREQWGKLGLSLDYQRDRFTLDEGLSKAVRKVF
                     VELYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAGEFAGMDRFEARKATVAKLQELGALV
                     EIENRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTADKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNESGEMYVGEEAPAGDGWVQDEDVLDTWFSS
                     ALWPFSTMGWPDENAADFQRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGSQPFKNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDAARENVAKVAAGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVMTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFAQYAEGSIVVAAYPVVNPAFENAEAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILIKTADSELETFFKANENYIRRFTNPEQLEISSSIAAPELAMSA
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFIANAKPEVVEK
                     EKEKQADYQAKYDATVGRIAEMKKLVK"
     gene            3765..>4237
                     /locus_tag="U1099_001859"
     CDS             3765..>4237
                     /locus_tag="U1099_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868143.1"
                     /translation="MDNNLVSLNYLIDQLNNLEDFSMSLNGEVPSEISELKSSVFNFS
                     QESALFMENFSQQGWCLYESMDFDIVQRVNSEYEKNGIDKAERILIDYFKNEVRKKVH
                     WIKKSSKVLLDRSHLIDVFFEEHFKGNYICSVPLGLIIVDGAVNDFTKNKDNKGL"
BASE COUNT         1315 a          834 c          972 g         1116 t
ORIGIN      
        1 ggggaccaat tacaagatat ttggatagac tctttaaata aggatgaact tgaagaggaa
       61 ttcggttcct acaaagttta ctatgatggt aatttgatta ttgaaaatgg tgcatcctat
      121 acaagcgagg gtgctactaa aaatcaaata atatctatga aagaaacgat tgtcactgaa
      181 ttagatacaa acaaggaatt caaaaaatac acatttaaaa aatattatat cccagacgag
      241 ggtacagatc caacagaaat taagattata gaagatggat ttgctattga aacggtgttt
      301 gaacaaaaag atttgatccc taatattgct tttggtccta ctctgtacac agtgagagcg
      361 gagtttataa aggaaaacaa taaactggta ttgaggaagg ggagcatgga ggtatacaaa
      421 tgaaaagttt gacacaaacc ttcaccaaga aacaaaaatt agtagtggtg attgtagtga
      481 tactatccgt agcaataggt ttttggaaat ggcgtgatac ttttttcaat acgtattatc
      541 ctgacgatac ttcaatccaa gggcagttgt ttcccaatgt tgcaaacacg aagattgaaa
      601 ccgttgagaa tgtcttgcca ttttcagatg tgaaatatga taagataatt atcagaattg
      661 gtagcctgtc ttcagaatac gatgattcta atcaatcatc gaaagcaaat aatatcataa
      721 acagaaaaac attagacgaa aatttcatac aatggctcat agaagcattt gatgaagagg
      781 aagctaaaaa aatatctgat catatcgaag tgtactatcg tggcgaaata ataagcgaaa
      841 ttagttatta acgttacaca aacctctctc gcccgtagat gcagggttca agaatgaaaa
      901 acgaaatagc cagcccaaag gactgactat ttctaggaga ttatatgaaa aagaaaagtt
      961 ttgctatttc tagcttgagg atagtataaa ccaattttct taaagaatac taaaggagaa
     1021 aaattaatgt caaaagaatt atcaccaaaa tataatccag ctgaggttga ggctggtcgt
     1081 taccaaactt ggttggacca agatgtcttt aagccgtcag gcgatgcgga ggctaagccg
     1141 tattctatcg tcattccgcc accaaacgta actggtaagt tgcacttggg acatgcttgg
     1201 gataccaccc ttcaagatat tattatccgc cagaaacgta tgcagggctt tgacaccctc
     1261 tggctgccag gtatggacca cgcggggatt gccactcagg ctaaggttga ggagcgcttg
     1321 cgtgagcaag gtgtgactcg ttatgacttg ggccgtgaga aattcctcga caaagtctgg
     1381 gaatggaagg atgaatacgc agcgaccatt cgtgagcaat ggggcaagtt gggtctgtct
     1441 ttggactacc aacgtgaccg ctttacgctg gacgaaggct tgtcaaaagc tgttcgcaag
     1501 gtctttgttg aactctacaa aaaaggctgg atctaccgtg gcgaatttat catcaactgg
     1561 gaccctgcag ctcgcacagc cctttcagat atagaagtta ttcacaagga cgtagaaggt
     1621 gccttttacc acatgaatta catgttggaa gacggttctc gtgcccttca agttgcgaca
     1681 actcgtcctg aaaccatgtt cggtgacgtt gcggtggcgg ttaacccaga agacccacgt
     1741 tacaaggact tgattggcaa aaatgttatc ctaccaatcg tcaacaagcc aatcccgatc
     1801 gttgcggatg agcacgcgga tcctgaattt ggtacagggg ttgtgaaaat cacacctgcc
     1861 cacgatccaa atgacttcct cgtaggtcaa cgccacaatc tgccacaagt caatgttatg
     1921 aacgacgatg gtactatgaa cgagttggca ggcgaatttg caggtatgga ccgctttgaa
     1981 gctcgtaagg caacggttgc taagttgcaa gaactgggag cccttgtgga aatcgaaaac
     2041 cgtgtgcatt ctgttggaca ctctgagcgt acaggtgtgg tagtcgagcc acgtttgtca
     2101 actcaatggt ttgttaaaat ggaccaattg gctaagaatg ccattgccaa ccaagacaca
     2161 gctgataagg tagaatttta cccaccacgt ttcaacgata ctttccttca atggatggaa
     2221 aatgtacacg actgggtgat ttctcgtcag ctttggtggg gccatcagat tccagcatgg
     2281 tataacgaat ctggcgaaat gtacgtcgga gaagaggccc cagcaggtga cggttgggta
     2341 caggatgagg atgtcctaga cacctggttc agctctgccc tttggccatt ctcaactatg
     2401 ggctggcctg atgaaaatgc ggcggacttc cagcgttact tcccaacttc aaccttggta
     2461 acgggctacg acatcatctt cttctgggtg tctcgtatga ttttccaatc gcttgagttc
     2521 acaggcagtc agccattcaa gaacgtgctg atccatggtt tgattcgtga cgaagaaggt
     2581 cgcaagatgt ctaaatcact cggaaacggg attgacccga tggatgtcat cgagaaatac
     2641 ggtgcggacg ctttgcgttg gttcttgtca aatggctctg cccctggtca agatgttcgt
     2701 ttctcttatg agaagatgga cgcttcttgg aatttcatca acaagatttg gaacatttcc
     2761 cgctacatcc tcatgaacaa tgaaggcttg actttggatg cggcgcgtga gaatgttgcc
     2821 aaagtcgcag ctggtgaggc tggcaacgtg acggaccgct ggattctcca caaccttaac
     2881 gaaaccatcg ctaaggtcac tgaaaacttc gacaagtttg agtttggtgt ggctggccac
     2941 atcctctaca acttcatctg ggaagagttc gccaactggt atgtggagct gaccaaggag
     3001 gtgctttaca gcgacaacga ggctgagaaa gtcatgaccc gctctgtcct tctctacacg
     3061 ctggaccaaa tccttcgcct cctccaccct atcatgccgt ttgtgacgga agagatcttc
     3121 gctcagtacg cagagggctc tatcgtggtg gcagcttacc ctgttgtcaa cccagccttt
     3181 gaaaacgctg aagctcacaa gggagtggaa agccttaagg atctgattcg ttcggtgcga
     3241 aatagcagag cagaggtcaa cgttgctcct tctaagccga ttaccatctt gattaagacg
     3301 gcagatagcg agcttgaaac cttcttcaag gcaaatgaaa actacatccg acgctttaca
     3361 aacccagagc agttggaaat cagctcaagc attgccgcac cagaactagc catgtcagcc
     3421 gttatcaccg gtgctgaaat cttcttgcca ctcgccgacc tcctcaacgt cgaagaagag
     3481 ttggctcgtc tagacaaaga acttgccaag tggcaaaaag aactagacat ggtcggcaaa
     3541 aaactcagca acgaacgctt catcgccaac gccaaaccag aagtcgttga aaaagaaaaa
     3601 gaaaaacaag ccgactacca agctaagtat gatgcgacag taggacgtat cgcagaaatg
     3661 aaaaaattgg ttaagtaggt agaatacgat aaaaagccca acctaaaatg gctgggtttt
     3721 gtggtataat ttctttaata tattatgtat aagaggccaa tcaagtggat aataatttgg
     3781 tatcattaaa ttatttaata gatcaactta ataatttgga agatttttct atgtcattga
     3841 atggagaagt gccgtcagaa atttctgagt taaaaagttc ggtttttaat ttttctcaag
     3901 aatctgccct atttatggaa aatttttctc aacaaggttg gtgtctctat gaaagtatgg
     3961 actttgatat agtgcagaga gtaaactcgg aatatgaaaa aaatggtatt gataaagcag
     4021 aaagaatact gatagattac ttcaaaaatg aagtaagaaa aaaagtgcat tggattaaaa
     4081 aaagttccaa agttctttta gataggtctc atttgataga tgtatttttt gaggaacatt
     4141 ttaaaggcaa ttatatttgt agcgtgccac ttggtttaat cattgttgac ggtgcagtta
     4201 atgattttac aaaaaataaa gataacaaag gtttgtt
//
LOCUS       DASFSW010000147         4214 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.148, whole genome shotgun sequence.
ACCESSION   DASFSW010000147 DASFSW010000000
VERSION     DASFSW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.148"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            25..1122
                     /locus_tag="U1099_001860"
     CDS             25..1122
                     /locus_tag="U1099_001860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868144.1"
                     /translation="MTNNQYKIKIHHKAENKKIYISIGINVGFAIRDFVYGNDVYSFK
                     PGALLFLAKYKEEFAKEFYNKKINISVYASQTIFSFISSSKKLIGDIDIFEKIKSKVP
                     SREEFPELFKQFKLDYTNNFSDEVKEEIIYSEFLHYHKRFSMDNFHKSIESLTFEDVE
                     EVQKYLFQKIRYKVYIYGNVEERIEDIQAWSVNGCSEFINPIILPLTDEEVVWKGIEL
                     NGISDRRSYLMHTNLFQIPIADAYLTYQILSQWYSDKKIQITIDSIEMGAILPNTDNI
                     EDFKLDLASIQLFKDNILRQYEYLSGRKQLFYSEYFMKLWLAGLDYLDFYLYLQKISS
                     EEIEDKMSTVLKVSQFCKVELSKQNMEIIDE"
     gene            1115..1399
                     /locus_tag="U1099_001861"
     CDS             1115..1399
                     /locus_tag="U1099_001861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868145.1"
                     /translation="MSEQILINPEGFYPEIDNLSAAQALITDEMFSIDKGELNLDTVS
                     EIDSLVKELNSLYVLYREHLGRDVKNLNVVIAEWMKVDASLAGKNLLGGD"
     gene            1401..2780
                     /locus_tag="U1099_001862"
     CDS             1401..2780
                     /locus_tag="U1099_001862"
                     /inference="COORDINATES: protein motif:HMM:NF016617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868146.1"
                     /translation="MTKKLDNTAISTLKTEFEASYSTAETSLYEIFKALSVLTQSVNF
                     RGAGAESHKLFLQEASLNYAYVLLEVTQTFKSYISEVEKGFLEYESEVSGKVNTDTVS
                     EYKTSLSGIAKQIELLKSDLTSPESIARSHGVSIVSTGAGNLATQFSTIDSNLEKVNQ
                     DLADTDSSLKGKATTVSEAISNLKSSLDSISSTYVTSTGKYNTSTIGQLTKQDWYLKG
                     NKDIFSDKQKSDPFVYNAGSASVLEGQYAVGLAKDTYLTTTGAVASGQYKAGIDGGEI
                     YLKSNGYVLGGSLEVQATSAIEVTAAASMASGNVDLRTGKYGWYASGGASGPSAQGKV
                     GIIDGLFSVSGQARFLFAEGTIGSYDNGEEFYYGFDASASLTEASANFDLFNVQIDGQ
                     KPVFAISAEPVLTGGGGATAAISGQSVYEDFLGLDWLDVDAVTFKVGGKAIIGGNISV
                     TIPWPDLNF"
     gene            2794..3333
                     /locus_tag="U1099_001863"
     CDS             2794..3333
                     /locus_tag="U1099_001863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868147.1"
                     /translation="MKNIILIVTSMFLLTACGNSSTNTETQSSVESSTSSSVEDKALT
                     LVHGKNTTQAGEVVQYVEDLLEKNPSLGAENAISLNYADATNSDGTTTYALFLVTNRT
                     ADRIEQNFDFTVNWSYDGTVIYENQKVLYEPDEYGVLEPNTTAIIFLPLPAESVDVVK
                     NMTDNSKISLSISDLAYVE"
     gene            3336..3842
                     /locus_tag="U1099_001864"
     CDS             3336..3842
                     /locus_tag="U1099_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868148.1"
                     /translation="MNKTWLYLLIFLPITVPFCFCLYRWRREYVIRKYSTSTIIGKVV
                     AHREWISLRPPIIEYEVKGIIYRKALEYTSVVYGPKDISYPRDIEELRNYMLERKKLY
                     NIGLMSYNMYEIFPIGYEMTVMYNPKRPQLAFVERYAGGELFFRFVSLFYFLVIILIC
                     LFLFFVLI"
     gene            3856..>4214
                     /locus_tag="U1099_001865"
     CDS             3856..>4214
                     /locus_tag="U1099_001865"
                     /inference="COORDINATES: protein motif:HMM:NF028198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HEM4868149.1"
                     /translation="MSKDAEIARCNQRILTTNSQISSTESTIATYKTKLEALKKAYEA
                     MKTTKSDFKDKKKSLSQLQRSADTSWSGNLFNTKYDKPLEDLISDVNDVIGKIDDNLD
                     RINNKINDYENKIMQKES"
BASE COUNT         1451 a          605 c          864 g         1294 t
ORIGIN      
        1 gaaaactgtg agcgaggggt ctaaatgaca aataatcaat ataaaataaa aattcatcat
       61 aaagctgaga acaaaaaaat atatatttca attggtatca atgttggttt tgctatacgt
      121 gattttgtgt acggcaatga tgtatattct tttaaaccgg gagcattgct tttcttagct
      181 aaatataaag aagaatttgc taaagaattc tataataaga aaattaatat ctctgtgtac
      241 gcttctcaaa cgattttttc attcattagt agttcaaaga agttgattgg tgacatagat
      301 atttttgaaa aaataaagtc aaaagttccg tcacgagaag aatttccgga gttatttaaa
      361 caatttaaat tagattacac aaataacttt tctgatgaag tgaaagaaga aataatttac
      421 tcagaatttc tacattatca taaacggttc tcaatggata attttcataa atccattgag
      481 agtcttacat tcgaagatgt agaggaagtc caaaaatacc tttttcaaaa aattcgatat
      541 aaagtatata tttatggaaa tgttgaagaa aggattgaag atatacaggc ttggtcggta
      601 aacggttgtt cagagtttat taatccaatc attctaccgc ttactgatga agaggttgtc
      661 tggaaaggta ttgaactcaa tggaatatca gataggcgaa gttatctgat gcatacaaat
      721 ttattccaaa ttccaatcgc agatgcttac ttgacctatc aaattctaag tcaatggtat
      781 agtgataaaa agatacaaat taccattgat tctatagaaa tgggagctat actgccaaat
      841 actgataata ttgaagattt taaactagac ctagccagta tccaactatt taaggacaat
      901 attttaaggc aatatgagta tttaagcgga agaaagcaat tattttattc tgaatacttt
      961 atgaaactat ggctggctgg tttagattac cttgacttct atctgtactt acaaaaaata
     1021 agttcggagg agattgaaga taaaatgtct actgtactaa aggtaagtca attttgcaaa
     1081 gttgaactat caaaacagaa tatggagata atagatgagt gagcaaatat taataaatcc
     1141 agagggattc tatccagaaa tagataattt gtcagcggct caagctctta taacggatga
     1201 aatgttttca atagataagg gtgagcttaa tctggataca gtttcagaga ttgacagttt
     1261 agtaaaggaa ttgaattcac tttatgtact ttatagagag catctaggtc gggatgtaaa
     1321 aaatctaaat gtagtaattg cagagtggat gaaggtagat gcctcgcttg caggaaaaaa
     1381 tttgcttggg ggggattaag atgacaaaga agttagataa tactgctata tcaaccctta
     1441 agacagaatt tgaagcttca tactccactg cggaaaccag tctttatgag attttcaaag
     1501 cactgtccgt tttaacgcag tcagtgaact ttcgtggagc aggtgcagaa tctcataaat
     1561 tgtttttaca agaagccagt ttaaactacg cctatgtttt attagaggtg acgcagacat
     1621 ttaaatccta tatctcagaa gttgagaagg gattcttgga gtatgagtcg gaagtttcag
     1681 gaaaagtgaa tactgataca gtttctgagt ataagacttc tttgtcaggt atagccaagc
     1741 agatagaatt actaaaaagt gatttgactt ctccagaatc aatcgcgaga agtcatggag
     1801 tgagcattgt ttcaactggt gctggaaatt tagcaactca attttctacg attgattcta
     1861 atttagagaa agtgaaccaa gatttagcag atacggatag ttccttgaaa gggaaagcaa
     1921 ctacagtttc cgaagcaatt tctaatttga aatcaagttt agatagcatt tcttctacct
     1981 acgtcacttc aacaggtaaa tacaacacct caactattgg tcagttaacg aaacaagatt
     2041 ggtatctaaa aggaaacaag gatatatttt cggacaaaca gaagtcggac ccctttgttt
     2101 acaatgcagg gtcggcctca gtgttggaag ggcagtatgc ggttggtttg gcgaaggata
     2161 cataccttac tacaacagga gctgtagcat ctggacaata caaagcaggg attgatggtg
     2221 gagaaatata tttaaaatct aatggatatg ttttgggagg tagtctagaa gttcaagcaa
     2281 cttctgctat tgaggtaaca gcagctgcta gtatggcatc aggtaatgta gacttgcgca
     2341 ccggaaaata tggatggtac gctagcggag gtgcaagtgg accatctgca caaggaaaag
     2401 ttggaattat agatgggcta ttttctgtct caggtcaagc taggtttctt ttcgcagagg
     2461 ggacaattgg ttcgtatgat aacggcgaag aattctatta cggatttgat gctagtgcca
     2521 gccttactga agcaagtgca aatttcgatt tatttaatgt acaaatagat ggacagaagc
     2581 cagtttttgc aatttcagct gaacctgttt taactggtgg aggcggagcg acggctgcaa
     2641 tttcaggtca atctgtatat gaagattttt tagggctaga ttggttagat gttgatgcag
     2701 tgacattcaa agtaggtgga aaggcaataa ttggtgggaa tatctcggtt acaattcctt
     2761 ggccagacct caatttttag aaagtaagaa atcatgaaaa atattatctt aattgtcaca
     2821 agcatgttct tattaacagc ttgtggaaat tcgagtacaa atacagaaac acaatcaagt
     2881 gttgaaagta gtacaagtag ttcagtggag gataaggcgt tgacattagt acatggaaaa
     2941 aatacaactc aagctggtga agttgttcaa tatgttgagg atttgcttga aaagaatcct
     3001 agcttaggag cagaaaatgc cattagttta aattatgcgg atgctacgaa ttcagatgga
     3061 accactacct acgccctgtt cttagtaacg aacaggacgg cagataggat tgaacaaaat
     3121 tttgatttta cggttaactg gtcctatgat ggaacggtca tctatgaaaa tcaaaaagtg
     3181 ctgtatgagc ctgatgaata tggagtgttg gagcccaata cgacagccat tatcttccta
     3241 cctctccctg ctgagagtgt tgatgttgta aaaaatatga cagataatag caaaatttca
     3301 ctttcgattt cggatttggc ttatgtggag taaaaatgaa caagacttgg ttatatcttt
     3361 taatattttt gccgataact gtaccttttt gtttttgtct ttatagatgg cgtagggaat
     3421 atgttattcg aaaatacagt acttctacaa ttatcggtaa agttgttgcg catagagaat
     3481 ggatttcact tcgtcctcca attatagagt atgaagtaaa gggtattata tatagaaaag
     3541 ccctagaata cacatctgtt gtttatggtc caaaagatat ttcatatcct cgtgatatag
     3601 aagaactccg aaattatatg ttggaaagga aaaagttata caacattggt ttaatgtcgt
     3661 acaatatgta tgaaatattt cctattggat atgaaatgac agtgatgtac aatccgaagc
     3721 gaccccagtt agcttttgta gaacgttatg ctgggggaga gttatttttt agatttgtca
     3781 gcttattcta ttttcttgta ataatcctta tctgtttgtt cttgtttttt gttttaatat
     3841 aaggaggtat ttctaatgtc aaaagatgct gaaattgctc gttgtaatca acggatatta
     3901 actactaata gtcagatttc aagtacagaa tcaacaatag ccacttacaa aacaaaacta
     3961 gaagcactta aaaaagctta tgaagctatg aagacgacca aatctgattt taaggacaaa
     4021 aagaaatcat tgagtcaatt acagaggagc gcggacacga gttggtctgg aaatttattc
     4081 aatacaaaat atgataaacc actcgaagat ttgatctcag atgttaacga tgttattggt
     4141 aaaattgatg ataatttaga tcgtatcaac aataagataa atgactatga gaataaaatc
     4201 atgcaaaaag aatc
//
LOCUS       DASFSW010000148         4196 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.149, whole genome shotgun sequence.
ACCESSION   DASFSW010000148 DASFSW010000000
VERSION     DASFSW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.149"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1161)
                     /locus_tag="U1099_001866"
     CDS             complement(<1..1161)
                     /locus_tag="U1099_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HEM4868150.1"
                     /translation="MEKQTFITLEDIDTSIAAENFNRLLDENKTYFLNGSWGSGKTTF
                     LESTESQSKKKFVTIDLWRNTDKRSLVEVAYASLHPVLYWGALLFSFLFVIISILMTN
                     TVNLGLSKFLHPIWMLPVGFISLVVAAVQLVAIKTEIVYSWLLANLCLKNKVLVIDDF
                     DRLTLSQQEEAYRLFSLLRGRLPIVFVGDIENIYSVKDNFLSKIIDRRVELPFVLQSE
                     QIWDDYFIKLEQRFATEISDSFKKRVKVEKRSLRDREHFNDYVNQEFFSRGKLGHVQV
                     EEQLLVIYSYLFYIEYYRKLLNNETIIENKSNSSDFSEIFTKGEEIPNLLSRLQRDNT
                     NKYPVSFKMNREGYFLYESPSNRKKAELDRILNGSEEQLSRELAYSNSGTDFY"
     gene            complement(1304..2041)
                     /locus_tag="U1099_001867"
     CDS             complement(1304..2041)
                     /locus_tag="U1099_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868151.1"
                     /translation="MNETRFLELFNDLDKVLRKVCGVADGEYADVGSMLTKAKELTRH
                     NPVEANWDKLYVARQLRNLMVHEKRTGLKEVAQPSQELITVLEKVIAQYQEPITIADY
                     LQKTQKIKPVIFEGKDKLIKALEIVEQEHFSKFPIFSDKVYQGLVSDKGLTNWLAKAS
                     KETGSIREKLVEASLEDVLACEEDSIQVIVLPITAHLYQVINHFEGRKRTTILVSRQA
                     SGKLHSPEDLVGIVTAQDLAEIYGMVD"
     gene            complement(2043..3077)
                     /locus_tag="U1099_001868"
     CDS             complement(2043..3077)
                     /locus_tag="U1099_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEM4868152.1"
                     /translation="MKTLDYIVTLTNTPSPTGFTTDIMNYIKAEVESFGYAASKTAKG
                     GVLVTVPGENDQEHRMVTAHLDTLGAMVRAVKPDGRLKMDLVGGFGYPSIEGENCLIH
                     CAKNGKTFTGTILMHQTSVHVYRDASTAERNQTNMEIRLDEKVTNADETRTLGIEVGD
                     FISFDPRTVVTETGFIKSRHLDDKVSAAILLHLLKVYKEEGVTLPYTTHFYFSNNEEI
                     GYGANSSIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDGSGPYHYELRQHLVALAE
                     RDGIPYKLDIYPYYGSDASAAMRAGADVKHALLGAGIESSHSYERTHLDSVVATERMV
                     DAYLKSAMVD"
     gene            3142..4098
                     /locus_tag="U1099_001869"
     CDS             3142..4098
                     /locus_tag="U1099_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEM4868153.1"
                     /translation="MKTYQKIYLLLKEREDYVSGEDLAQELGISRTSIWKAIRQLETH
                     GLTIEAARNRGYRLAEGDLLLPELISQELQLPVHLNMDSDSTQLDAKQGIESGHTSPA
                     LYLAPYQNKAKGRFGRPFYASKSGGIYMSLRLSPNVPFLEFKPYTILAAAAVVKAIQS
                     LCDLDVQIKWVNDIYLGHKKVAGILTEAISSMENQRVTDVIIGVGINVRIDDFPKELQ
                     QSAGNLFEEQPPFTRNQLITAIWKAFLETDEKELIALYKEKSLVVGQQVSFVENQVEF
                     KGTAIAVTDTGNLVIQLDNGKAKIISSGEISLTSWSASPPNE"
     gene            complement(4064..>4196)
                     /locus_tag="U1099_001870"
     CDS             complement(4064..>4196)
                     /locus_tag="U1099_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3272 family protein"
                     /protein_id="HEM4868154.1"
                     /translation="MNASILTGDFLIAGIYAFIAYRNLHFAYKVTKFIRLVEKQTKK"
BASE COUNT         1255 a          957 c          733 g         1251 t
ORIGIN      
        1 ataaaaatct gtaccactat ttgagtaagc taattcccta gacaattgtt cttctgatcc
       61 atttaatatc ctatctagtt ctgccttttt cctatttgaa ggcgattcat aaagaaaata
      121 tccctctcta ttcatcttaa aacttactgg atatttgttt gtgttatcac gttgtaggcg
      181 agacagaagg ttgggaattt cctctccttt agtaaatatc tcactaaaat ctgacgaatt
      241 gcttttattt tcaatgatcg tttcattatt gagtaacttc ctatagtatt caatatagaa
      301 aagataggaa taaatgacta ataattgttc ctctacctga acatgcccaa gttttcctct
      361 ggaaaaaaat tcttggttaa catagtcatt gaaatgctct ctatccctta gacttctctt
      421 ttctaccttt actctttttt tgaaactatc agaaatttca gtggcaaatc tttgttctaa
      481 ctttataaaa taatcatccc aaatctgttc agattgaagg acaaacggca actctaccct
      541 cctatcaata atttttgata gaaaattatc ttttacacta taaatattct cgatatcacc
      601 aacaaagact ataggcaacc tccctcttaa caaggaaaat aatctatatg cttcctcctg
      661 ttgtgagagc gttaaacggt caaagtcgtc aatcactaga actttatttt ttaagcacaa
      721 attagccaat aaccaagaat aaactatttc tgtctttatc gctactaatt gcaccgccgc
      781 aactactagg gaaataaacc caacagggag catccatatg gggtgtaaaa atttacttaa
      841 tcctagattt actgtattgg tcattaaaat agagatgatt acaaaaagaa aactgaatag
      901 taatgctccc cagtataaaa caggatgtaa actggcataa gcaacttcaa caagacttct
      961 tttgtcagta tttctccata aatcaatagt aacgaacttc ttttttgatt gcgactctgt
     1021 tgattctaaa aacgttgttt ttcctgatcc ccacgaacca ttcaaaaagt atgttttatt
     1081 ttcatccaaa agtctattaa aattttctgc tgcaatagag gtatcaatat cttctaaagt
     1141 gataaaagtt tgtttttcca tattttttcc taaaaaatat ttatattgtg acaatgtaga
     1201 attgggactc ctaaatatct tttatttacg agtttcacaa catattcaag tctttttcaa
     1261 cacagtaata caccttgata atggattaac aagaactatt gcttcaatcc accatcccat
     1321 aaatctctgc caagtcctgg gctgtgacaa taccaacaag atcctctggc gaatgaagct
     1381 tcccactcgc ctgacgactg actaggatag ttgtgcgctt acgcccctca aaatgattaa
     1441 ttacctgata aagatgggct gttattggta gaacaattac ctgaatacta tcttcctcac
     1501 aagctagtac gtcttcaaga ctagcctcca ccaatttttc acgtatagag cccgtttcct
     1561 tgcttgcctt ggccaaccaa tttgtcaatc ccttgtcaga aaccaggcct tgataaacct
     1621 tgtcagaaaa aataggaaat ttagaaaaat gctcttgctc cacaatttct aaagccttta
     1681 tcaatttgtc cttgccctca aaaataaccg gcttaatttt ctgggtcttt tgcaaatagt
     1741 cggcaatagt aatcggttct tggtactggg ctatcacttt ttccaaaaca gtgatcaatt
     1801 cctgcgaagg ttgggctact tccttcaaac ctgttcgttt ctcatgaacc atgagattac
     1861 gcaactgacg agccacatag agtttatccc agttcgcctc aacgggatta tgtcgcgtca
     1921 attcctttgc cttggtcaac atggaaccaa cgtccgcata ttccccatcc gcaacaccgc
     1981 acaccttacg caagacctta tccaagtcat tgaataactc taaaaaacgg gtttcattca
     2041 tcttaatcta ccattgctga tttcaaatag gcatctacca tccgctcagt cgcaactaca
     2101 gaatccaaat gggtccgctc gtaggaatgg ctagactcaa tgccagcacc aagcagtgca
     2161 tgcttgacat ctgcacctgc tcgcatagct gccgaagcat cggaaccata gtaaggataa
     2221 atatccagct tataaggaat tccatctctt tcagccaagg ctaccaagtg ctgacgaagt
     2281 tcataatggt agggacctga gccgtccttg acacagatag aaactgtata ttcgtcagtt
     2341 tgctggtcat ctcccattgc ccccatatcg actgccaaat attcaaccac ctgagcagga
     2401 atgctggagt tggcaccgta gccaatctct tcgttgtttg aaaaatagaa atgtgtcgta
     2461 taaggcaggg tcactccttc ttccttatag accttcaaaa gatgaagcaa gatagctgcg
     2521 gataccttat catccaagtg acgactcttg ataaagccag tttctgtcac gacggtacga
     2581 ggatcaaagg agataaaatc tccgacttca atacccaagg tccgcgtctc gtccgcattg
     2641 gtcacttttt cgtccaaacg aatttccatg ttggtctgat tgcgctcagc ggtactggca
     2701 tcccgataga catggacaga ggtctggtgc atgagaatag tacctgtgaa agtcttgcca
     2761 tttttagcgc aatgaattaa gcagttttct ccctcaatag aagggtatcc aaatcctccc
     2821 accaaatcca ttttaagtcg gccatctggt ttcactgcac gaaccatggc ccccagtgtg
     2881 tctaggtgag cagtcaccat gcggtgttcc tggtcatttt cccccggaac tgtcaccaaa
     2941 acaccacctt tagcggtctt actagcagca tagccgaaac tttcaacctc tgccttgatg
     3001 taattcataa tatccgtcgt aaaaccagtt ggcgacggag tgttggtcaa tgtaacaatg
     3061 taatcaagtg tcttcatgaa aacctcctta tttgaaacta ttatatcacg ttttaggtga
     3121 attttttgct ataatgaagg tatgaaaacc tatcagaaaa tatatctcct tttaaaagag
     3181 agagaggatt atgtcagcgg agaagatttg gcacaggaat tgggcatttc acggacatcc
     3241 atttggaagg ccattcgcca gttagaaacg catggtttaa ctattgaggc tgcccgtaat
     3301 cgtggctaca gattagccga aggcgattta ctcctacccg aattaatctc tcaggagctt
     3361 caactacctg tccatctgaa tatggatagc gattccaccc aactagatgc caaacaaggt
     3421 attgaatcag gtcatactag tcctgcccta tatttagctc cctatcagaa taaagccaag
     3481 gggcgatttg ggagaccttt ttatgcatcc aagtcaggtg gcatttatat gtcgcttcgt
     3541 ctctctccca atgttccctt tctagaattt aagccctaca ccattttagc tgcagctgct
     3601 gttgtgaagg ccattcaatc tctttgcgac ttggacgtcc aaatcaagtg ggtcaatgac
     3661 atctatctcg gacacaaaaa ggtcgctggc atcctcactg aggctatttc ttctatggaa
     3721 aaccaacggg taaccgatgt cattatcggt gtcggcatca atgtccgcat agacgatttc
     3781 cctaaggaat tacaacaatc tgctggaaac cttttcgagg aacaaccacc atttacccgc
     3841 aatcaactca ttactgccat ctggaaagcc ttcttagaaa ccgatgagaa ggaactaatt
     3901 gccctttaca aagaaaaatc acttgtcgtt ggccaacaag tgagctttgt agaaaatcaa
     3961 gttgaattta aggggactgc catcgctgtt actgatacag gaaacctggt tatccagtta
     4021 gataacggca aagcaaaaat tatctccagc ggagaaatca gccttacttc ttggtctgct
     4081 tctccaccaa acgaataaat ttagtcacct tgtaagcaaa atgaagattc cgataggcga
     4141 taaaggcgta gatacctgca atcaagaaat ctcctgtcaa aatcgatgca ttcata
//
LOCUS       DASFSW010000149         4130 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.150, whole genome shotgun sequence.
ACCESSION   DASFSW010000149 DASFSW010000000
VERSION     DASFSW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.150"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1440
                     /gene="ezrA"
                     /locus_tag="U1099_001871"
     CDS             <1..1440
                     /gene="ezrA"
                     /locus_tag="U1099_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEM4868155.1"
                     /translation="GYNNAFRFVSAKNAIDSIESQIDLIEEDIASIRHGLMELKEQEE
                     KNSGRVKHALNLFDSLQEAVRENPDSYGETLPELEKQLKNIEVEFSEFVMLNSSGDPI
                     EASEILDKTEEHMIALNQIMDRIPSLIERVTKDFPEQLEDLESGYRKLVEQNYLFTEA
                     NIESQFQNIRVSIRENTALIVSFDLDAAEAENEGIQAKIDHLYKVFNREIEANKEAVK
                     ISKNLPKFLEHVVQNTQLLDEESQRLNATYLLADSKLSRINQLKARLESIEIVVTESV
                     EDIENPQVAYSILEERLDHSLASLKEIEEEQLVLADYLKSQELSENTARKKATLYINK
                     LHTLKRYMEKRNLPGIPAEFLTNFFRTSDHVEALIAELDYKRINIEVVNRLLENATYD
                     MNQLEELAYLIVQNATLTEQLLQYSNRYRSFDESVQKAFNRSLSIFEKDFDYQAAFEE
                     ISFALETVEPGVTERFVRSYEKTREAIRY"
     gene            1492..2127
                     /gene="serB"
                     /locus_tag="U1099_001872"
     CDS             1492..2127
                     /gene="serB"
                     /locus_tag="U1099_001872"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEM4868156.1"
                     /translation="MTTGLLVMDVDSTLILEEGIDLLGEEAGLGAQVAAITERAMRGE
                     LDFEEALRERVALLKGLPVSVFDRIIKKIHFTPGAAELVSELKMRGYKVAVVSGGFHE
                     TVDRLAAQLELDYVRANRLEVVDGVLTGQVLGEIVTKDTKKACLEEWAAENGLSLSQT
                     IAMGDGANDLPMIQRAGIGVAFCAKPIVQEQAPYQINEKNLYKLIEILDSK"
     gene            complement(2158..2808)
                     /locus_tag="U1099_001873"
     CDS             complement(2158..2808)
                     /locus_tag="U1099_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4868157.1"
                     /translation="MYQTILFDLDGTLTDSGQGILNSVAYALEKMGIEEPDTANLNRF
                     IGPPLYESFSRFYQLNPEDTQSAVDTFRVYFKEKGMFENQLYPGIIPLLEELRTAGKT
                     LVIATSKPEIFAKQILEHFGIAHYFDIIAGASLDSSRISKADVIGYAINQLEAFPKHA
                     VMIGDREHDIEGARMHQLPAIGVLYGYGNKQEFEKAGATMIVETVQDLKRVLLTTE"
     gene            2916..4043
                     /locus_tag="U1099_001874"
     CDS             2916..4043
                     /locus_tag="U1099_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM4868158.1"
                     /translation="MHILIAPDSFKESLSATKVAEAIKKGFSKVYPQAGYDLIPLGDG
                     GEGTVESLMQALSLDGTQTWVTGPFGDKIKVSYAVKDDLAVFEMAEIVGLASIPHERR
                     SPLFIKTQGVGELIVELVQNGVKRIFIGVGGSASHDGGIGMAAGLGVKFYNREGKEVE
                     AIGAHIGEIVSFSTEDMLVDLSQVRIDLVTDVENPLCGPAGATFVFAGQKGLAPAEFE
                     LVDKKMESFYKLVNPMLLDLAGAGAGGGMAAGLVQFAGARIQKGIDFVLDQLDFAHRV
                     TKADLVVVGEGRMDAQSLSGKTPIGVARRTPTGIPIIAICGSLKDDLPEFPFENICAA
                     FPIIASVESLEDTLQKAEKNLVRTAEQVARILQLGGQQRWN"
BASE COUNT         1254 a          705 c          980 g         1191 t
ORIGIN      
        1 ggctataaca atgcctttcg ctttgtgagt gcaaagaacg ctatcgacag tatcgaaagc
       61 caaattgatt taattgaaga agacatcgcc tctatccgtc atggtttgat ggagttgaag
      121 gagcaagaag agaaaaactc tggtcgcgta aaacatgcgt taaacttgtt cgatagttta
      181 caggaagctg ttcgtgaaaa tccagatagc tacggtgaga cactaccaga acttgagaaa
      241 caattgaaaa atattgaagt tgagttttct gaatttgtga tgctcaattc ctctggtgac
      301 ccgattgaag cttctgaaat ccttgataag actgaagagc atatgattgc cctcaatcaa
      361 atcatggatc gcattccaag tttgattgaa cgagttacaa aagatttccc agagcaattg
      421 gaagatttgg agtcgggtta tcgtaaactg gttgagcaga attatttgtt cacagaagct
      481 aacatcgagt cacagttcca aaatattcgc gtttcaattc gtgaaaatac tgctttgatt
      541 gtttcctttg atttggatgc cgcagaagct gaaaatgagg gaattcaagc aaaaattgat
      601 catttgtata aagtcttcaa tcgtgagatc gaagcgaata aggaagctgt taagattagt
      661 aaaaacttgc caaaattctt ggagcatgtt gtccaaaata cacagctctt ggatgaggaa
      721 agccaacgcc taaatgcaac ttacttgctg gcagatagca aactttcacg gatcaatcag
      781 cttaaagctc gtttggaatc gattgaaatt gttgttacag aaagtgtgga agacattgaa
      841 aatccacaag ttgcatattc tattttggaa gagcgtttgg accattcatt ggcaagcttg
      901 aaagaaatcg aagaggagca gcttgtttta gctgactatc ttaaatcaca agaattgtca
      961 gagaacacag cgcgtaagaa agctacactt tatatcaaca aattgcatac ccttaagcgt
     1021 tatatggaaa aacgcaacct tccaggaatt ccagctgaat ttttaacaaa cttctttaga
     1081 acgagcgatc atgtggaagc cttgattgcc gagttagact ataaacgaat caatatcgaa
     1141 gttgttaatc gtttgttgga aaatgcaacg tatgatatga atcaattgga agagttggcg
     1201 tatttgattg tgcaaaatgc gactttaaca gagcagttgc tacaatactc taaccgttat
     1261 cgttcattcg atgaaagtgt gcaaaaagca tttaaccgtt cattatcaat ttttgaaaag
     1321 gattttgatt accaagcagc atttgaagaa atttcatttg ctctggaaac agttgaacct
     1381 ggtgtaacag agcgttttgt acgttcatac gaaaaaactc gcgaagccat tcgctactag
     1441 ataaaaagcc atttggcttt ttatttttat actttgaaag gaatattgga gatgacaact
     1501 ggattattag ttatggatgt tgattcaaca ttaattttgg aagaaggaat agatttactt
     1561 ggagaagagg ccggtttagg agcgcaagtt gcagcaatta ccgaacgtgc tatgcgtggg
     1621 gaattggatt ttgaagaagc cttacgtgaa cgtgtggcgt tgttaaaggg actgcctgtt
     1681 tctgtgtttg atcgaattat caaaaaaatc cactttacgc ctggagcagc agaattggtc
     1741 tcagaattaa aaatgcgagg ctataaggta gcagtagttt caggcgggtt tcatgaaaca
     1801 gtcgatcgac tagctgcaca gttagagtta gactatgttc gggctaatcg attagaagta
     1861 gtggatggag tattgacggg acaagtattg ggtgaaattg taacgaaaga tactaaaaaa
     1921 gcatgtttag aagaatgggc ggcagaaaat gggctgagtc tatctcaaac gattgccatg
     1981 ggggatggag caaatgatct accaatgatt caacgagcag gaattggagt agctttctgt
     2041 gctaaaccga ttgtccaaga acaggcgccc taccaaataa atgaaaaaaa tctctataaa
     2101 ctaatagaga ttttagatag taaataacga gaagtcagct gagtgctgac ttttcttcta
     2161 ttctgttgtc agtaaaaccc ttttcaaatc ttggacggtt tcgactatca tggtagcccc
     2221 agccttttca aattcctgct tgttcccata gccgtaaaga acaccgatgg cgggaagttg
     2281 gtgcatgcgt gctccttcaa tgtcatgttc cctatctcca atcatcacag catgttttgg
     2341 aaatgcttct aattgattta tagcatagcc gatgacgtcc gccttactga tgcgactact
     2401 atccagacta gcaccggcaa tgatatcaaa gtagtgagcg ataccaaaat gttctagaat
     2461 ctgtttggca aatatttctg gctttgaggt agcaattact agtgtttttc cagcagttct
     2521 caattcttca agcagaggga tgattccagg atagagttga ttttcaaaca ttcctttttc
     2581 tttaaaatag acacggaaag tatcaactgc actttgtgta tcttcagggt tgagttggta
     2641 gaatcgtgaa aaagattcat agagtggcgg gccgatgaat cggtttaaat tggctgtatc
     2701 tggttcttcg attcccattt tttctagtgc gtaggcgacg gagtttaaga ttccttgtcc
     2761 agaatcagta agtgttccat ctaagtcaaa taatatagtt tgatacatac ttatctcctt
     2821 tctttcagta taacacagac gatgaaaaac agaaaagatt tattgaaagc tgaataggag
     2881 cgtgatatac taataatata aaggaggcta gtaccatgca tattctcatt gctccggatt
     2941 catttaaaga gtctctttca gctacaaagg ttgctgaagc gattaaaaaa ggattttcta
     3001 aggtttaccc acaagcaggg tatgacttaa ttcccctagg agatggtggc gaagggacag
     3061 ttgagagtct aatgcaggcc ttatccttgg atgggaccca gacttgggtg acaggtccat
     3121 ttggagataa aattaaggtt tcctatgcgg ttaaagatga tcttgcggtt tttgaaatgg
     3181 ctgagattgt agggctggca agcatcccgc atgaaagaag gagtcctctc tttattaaga
     3241 cacaaggggt tggggagctg attgtggagc tcgttcaaaa tggtgtcaaa cgcatattta
     3301 taggagtagg tggctctgcc agtcatgatg gtggtatcgg aatggcggca ggtttaggag
     3361 tgaaatttta taatcgagag ggaaaagaag tagaggcaat tggagctcat atcggggaga
     3421 ttgtcagctt ttccacagag gatatgttgg tagatctatc tcaggtcagg attgatttgg
     3481 taacggatgt ggagaatccg ctttgcggac cagcgggtgc cacatttgtt tttgcaggtc
     3541 agaaaggtct tgcccctgct gaatttgaac tagtagacaa gaaaatggag tccttttata
     3601 aattagttaa tccaatgcta ctcgatttgg caggagcggg tgctggagga ggtatggcag
     3661 caggtttagt ccaatttgct ggtgctcgca ttcaaaaagg catcgacttt gtcttggatc
     3721 agttggactt tgctcatcgc gtcaccaaag cggatctggt tgtggtcgga gaagggcgga
     3781 tggatgccca aagcttatca gggaagactc cgataggagt agctagacgg acgccgacgg
     3841 ggattcccat cattgcaatt tgtggtagcc tcaaagacga tcttcccgag tttccttttg
     3901 aaaatatctg tgccgctttt ccgataattg caagcgttga aagtttggag gacacgcttc
     3961 aaaaagcaga aaaaaacctg gtccgcacgg cagagcaggt ggcaagaatt cttcagttag
     4021 gaggtcaaca aagatggaat tagtgaaaga tgtggctatt atcttattta tttttatttg
     4081 tcttgcaaag ctagtatccg ttatgagact ttccgaaaaa gaaagaggaa
//
LOCUS       DASFSW010000150         4116 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.151, whole genome shotgun sequence.
ACCESSION   DASFSW010000150 DASFSW010000000
VERSION     DASFSW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.151"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            273..599
                     /locus_tag="U1099_001875"
     CDS             273..599
                     /locus_tag="U1099_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM4868159.1"
                     /translation="MHFPVPAVLTEFLIMAILESDDSYGYEICQTIKLIANIKESALY
                     PILKRLEQNDFLTTYSQEYQGRMRKYYSLTQLGHEELVRLKDDWDTYTNTINGIIEGS
                     VRHDKN"
     gene            586..1341
                     /locus_tag="U1099_001876"
     CDS             586..1341
                     /locus_tag="U1099_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEM4868160.1"
                     /translation="MTRTEYMEQLEKYLKKLPHKEYFEAISFFNEYFDEAGPERETEI
                     IEELGSPKEAASELINNMLNKQIQEEKEQQEPIQLNWKLWVGLGALSMTGLFSFFLLF
                     IMGEFIGVIPLFATLILGAFFLGRYFRNFSQTKRTLWLAILAVISLPIAIPLLLILLA
                     SLLGLVALILALIVGAFVLGVGLLTSGGYLIWEAFSLMSEGFNIFLMGFGSGLSLIGG
                     AILIYILTGFFAYWSWRLVKACFKWILKRGKRA"
     gene            1338..2396
                     /locus_tag="U1099_001877"
     CDS             1338..2396
                     /locus_tag="U1099_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEM4868161.1"
                     /translation="MKKKLTIAIIIGFVSLIAGLILAGIGFFTGGITRLEEVAAPTEV
                     HKTFTDLNTIKIDFIPHSVYIKESSDNNYHVTYANSDNNIQKPLKLSEKDGVLTLSAQ
                     ELEFAIEGIMQYLGESLAQRRINVFSVTIEVPKGKTLDKLEGHNLHFYDFGGFTIENI
                     HIKEANLSGGVTLDKVTIDSGQIEAGYFQATQSTLKDMHINLQGSSVRLYETSLENVT
                     IEGYSQLDSRQTTLLGDNRFTPSDTYSSTTFLDVTDKSLADSNLLITNQIDKKKLVEA
                     QGYYYEDGNDLGEMVNQDPYLKERLGEVGIFTSDKYTKFPVKTENDLQTLTLENKGSK
                     NKLTIEATNATINLGTPK"
     gene            complement(2448..>4116)
                     /locus_tag="U1099_001878"
     CDS             complement(2448..>4116)
                     /locus_tag="U1099_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEM4868162.1"
                     /translation="ANSSQIIAGVGQLQSGASQVEQLVTGANQLQAGLEQLASSTSLS
                     AEQSSQIQALLTGLPQLQAAINQLNDSLSSIGGLAVDTSTLSSLLTEMGAQAQGLLTA
                     AQADKTAGIEALQATATYQHLPADQQAELVGALQNSPSATAAVAQTILIQLSQLSQTL
                     SSLQSLSGMETQMSQLQSAVGQINTAANQALPGATTAIENLSSGLSQVNTALNQQVLP
                     GTQVLTGGVSQLQTQLSSGASQLMSGVTAYTAGVAQLAAGGAQLVANNSSIQSGGGQL
                     TSGLATLASNSSQLVSGSGQLALGSQQLITGADQLASGGQTLTSGITSLRTGSETLTN
                     SLSSASQQLSVVSVEDKNAQAVSQPVTLEHSDQDDVKTNGVGMAPYMVSVALMVAALS
                     ANVIFVKHIDNRSYKNRWDWAKGKLLLNGIIASLAAVILYGVLRLIGIEPAHPMATLG
                     LILLASWTFMALVTALVGWNNRFGSFASLIILLLQLGSSAGTYPIELSPRFFQVIQLY
                     LPMTYSVSGLRQTVSMVGNSSHQVWMLSLFLVGFMGLGLLIYNQKDE"
BASE COUNT         1169 a          926 c          890 g         1131 t
ORIGIN      
        1 gtcattctct tttagtccag aaattccctt aatgttagta ctgttattaa gtttactact
       61 ctgaaaatat ttttcaaggc tataaaacgt ttacacctta aaaatttgtc ctaagctatt
      121 tggtcttaag ctattttatt tattttgcac ataatcactt gacaatttat ttttgtaggt
      181 gtatatttaa tatataacaa aattttatat atacaactga acgatatata tcaaaaccga
      241 acctaactaa caacaaagga aaggagcgtc ctatgcattt tccagtcccc gcagtgctga
      301 cagaatttct catcatggct attttggaat ccgatgattc ttatggctat gaaatctgtc
      361 agaccattaa attaattgcc aacatcaagg agtccgccct ctatcccatc ctaaaacgat
      421 tggagcaaaa tgactttttg actacttatt cccaagaata tcagggtcgt atgcgcaagt
      481 attatagcct aactcagtta ggtcacgaag aattggtccg actcaaagat gactgggata
      541 cctatacaaa cacgataaac ggcatcatag aagggagcgt ccgccatgac aagaactgaa
      601 tacatggagc agttagaaaa atatctcaaa aaactgcccc acaaagaata ctttgaagcc
      661 atcagcttct ttaatgagta ttttgacgag gctggaccag agcgtgaaac agaaattata
      721 gaagaacttg gttcaccaaa agaagctgcc agcgaactca ttaacaacat gctcaacaag
      781 caaatccagg aggagaagga acaacaagaa cctattcaac tcaactggaa actctgggtt
      841 ggtctgggag ccttaagtat gacaggtctc ttctctttct tcctactctt tatcatggga
      901 gagtttatcg gagtcattcc tctgtttgcg actcttatcc tcggcgcctt tttcctcggt
      961 cgctacttcc gtaattttag ccaaacaaaa cgaacgcttt ggttggctat cctagctgtc
     1021 atttccctgc ctatcgccat tccacttttg ctcattctcc tagctagtct attgggattg
     1081 gtggcgctca tcttggccct cattgtcggt gcctttgttt tgggtgtcgg acttctcacc
     1141 agcggtggct atctaatttg ggaagccttt agccttatgt cagaaggttt caatatcttc
     1201 ctcatggggt ttgggtcagg tttgtctttg attggcggag ctattctcat ctacatcttg
     1261 actggatttt ttgcctactg gtcttggcga ctggtcaagg cttgcttcaa atggatcttg
     1321 aaacgaggta aacgagcatg aaaaagaaac ttaccatcgc cattattatc ggctttgtca
     1381 gcctgattgc aggcttaatt ctagctggca ttggattttt caccggaggt ataacccgct
     1441 tagaagaggt tgctgctccg acggaagtcc ataagacctt cacagatttg aataccatta
     1501 aaattgattt tatcccccac agcgtctaca tcaaagagtc tagtgataac aactatcatg
     1561 tgacctatgc caactccgac aacaatatcc aaaaacctct aaaactcagt gagaaagacg
     1621 gcgtcttaac tctctctgca caagaactag aatttgccat tgagggaatc atgcagtatt
     1681 tgggagaaag cttggcccag cgtcgtatca atgtcttctc cgtcaccatt gaagtgccta
     1741 aagggaagac actggataaa ttagagggac acaatctcca cttctatgat tttggcggtt
     1801 tcacgattga gaatatccat atcaaagagg caaatctgtc tggtggtgtc actcttgaca
     1861 aagtgactat tgacagcggt cagattgaag caggctactt ccaagcgaca caatccactc
     1921 taaaagatat gcacatcaac ctacagggaa gcagcgtccg cctgtatgaa accagtttgg
     1981 aaaatgtgac catcgagggc tatagccagc tggatagcag acaaacgact cttcttggcg
     2041 ataatcgttt cactccatcc gacacctact cctctacgac atttttggat gtgacggaca
     2101 agagtctggc agatagcaac ttgctcatca ccaaccagat cgacaagaaa aaattagtag
     2161 aagcccaagg ttactactat gaagatggga atgatttggg agagatggtt aaccaagacc
     2221 cttacttaaa agagcgacta ggggaagtcg gtattttcac tagcgataag tacaccaaat
     2281 tccctgtcaa gactgaaaac gatctacaga ccctaaccct agaaaacaaa ggtagcaaga
     2341 acaaactgac cattgaagcc accaacgcga caatcaattt agggaccccc aaataactac
     2401 atcaaccaaa aaccaactgt tcacacattg agcagttggt ttgcttatta ttcatctttt
     2461 tgattataaa tcaagagacc tagtcccata aagcctacta agaagagact cagcatccaa
     2521 acttgatgac tactatttcc aaccatagaa acggtttgac gaagacctga aacagaatag
     2581 gtcattggaa gatagagctg aatgacttgg aagaagcgtg ggctgagttc aattggatag
     2641 gtacctgcgc ttgagccaag ttgtagtaac aagataatta aacttgcgaa ggatccgaaa
     2701 cgattgttcc agcccaccaa ggctgtaacg agggccatga aggtccaaga ggctaagagg
     2761 attaatccca aggttgccat tggatgagcc ggttcgattc caatcaagcg aagaacaccg
     2821 tacagaatca ctgctgctag actggcaatg atcccattga gcaagagttt tcctttggcc
     2881 cagtcccaac gattcttgta ggagcgatta tcaatgtgtt tgacaaaaat aacattcgct
     2941 gataaggctg caaccattag ggcaacagac accatgtaag gagccatgcc gactccgttg
     3001 gtcttcacat catcttggtc actgtgttcc aaggtgactg gttgagaaac tgcttgtgca
     3061 tttttgtctt ctacagagac gactgatagt tgttgactgg ctgagctgag tgaattcgtt
     3121 agggtttcac tacctgtgcg gaggctagta ataccgcttg tcaaggtttg accgccacta
     3181 gctaattgat ccgcaccagt aattaattgc tgactaccta aagccagttg tcctgaaccg
     3241 ctgacaagtt gactggagtt ggatgccaag gttgccaatc ctgatgttag ctggccaccc
     3301 ccagattgga tactactatt attcgcaacc aactgggctc cacctgctgc taattgggca
     3361 acaccagcgg tataggctgt tactcctgac atgagttgac tcgctccgct tgacagttgc
     3421 gtttgaagtt gacttactcc acctgttagc acctgagttc ctggtagaac ttgctggttc
     3481 aaggcggtgt tgacctgact caagccactt gataggtttt caatggctgt tgtggcacct
     3541 ggcaatgctt gattggcagc cgtgttgatc tgaccgacag ctgattgtag ctggctcatt
     3601 tgtgtttcca tgccagaaag actttgtaag ctagaaagag tttgactgag ctgagacaat
     3661 tgaatcaaaa tcgtttgggc tactgccgct gttgccgaag gtgaattttg gagggcgcca
     3721 accaattcag cttgctgatc agctggcaag tgttgatagg tagccgttgc ttgcaaggct
     3781 tcaatgccgg ctgttttatc agcttgggct gctgtcaaca agccttgagc ctgggccccc
     3841 atctccgtca agaggctaga tagagtagag gtatcaactg caagtcctcc gatacttgat
     3901 aggctatcat ttaattgatt gatggccgcc tgcaactgag gaagtccagt caataaggcc
     3961 tgtatctgac tagactgttc agcagacaaa ctagttgaac tagcgagttg ctccaagcct
     4021 gcctgcaatt gattggcccc tgttaccagt tgttcaacct gacttgctcc tgattgcaat
     4081 tgtcccaccc cagcaatgat ctgagaggaa ttggca
//
LOCUS       DASFSW010000151         4070 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.152, whole genome shotgun sequence.
ACCESSION   DASFSW010000151 DASFSW010000000
VERSION     DASFSW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.152"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            378..887
                     /locus_tag="U1099_001879"
     CDS             378..887
                     /locus_tag="U1099_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868163.1"
                     /translation="MLKYIRALPLVIVAGAVVYFTIFICIDNGDWTYVVTRALGLKEE
                     HANNVSLIVIGTQSLFLTSDWFYKRIRAFLNRRLEKAKGLNRQIYLGYKAGIEFLMAL
                     PKRTILFTFYLLLVVAESVGLIEKAKDYAAVIIFIIAVDRVTKIWPEEKRRMIEFSQK
                     IKKRIKSVG"
     gene            1310..2587
                     /locus_tag="U1099_001880"
     CDS             1310..2587
                     /locus_tag="U1099_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEM4868164.1"
                     /translation="MSTKANLLFDVHEKPAPLQGILLSFQHVFAMFGATILVPLILGM
                     PVSVALFASGVGTLIYQVATQFKVPVYLGSSFAYISAMALAIKEMGGDVSAAQTGILF
                     VGLIYVLIAALVKVIGTKWIDSLLPPIVIGPMIIVIGLGLANSAVTSAGFVADGDWKN
                     VVVAIATFLIAAFVNTKGKGFAKIVPFLIAIIGGYVIAIFLGLVNFTPVLEAAWFELP
                     GFYLPFETGAFKSYNFYFGPEMIAILPIAVVTVAEHIGDHTVLSQICGRQFLKDPGLS
                     RTLIGDGVATAVSAFIGGPANTTYGENTGVIGMTRIASVSVIRNAALIAIAFSFLGKF
                     TALISTIPSAVLGGMSILLYGVIASNGLKVLIESRVDFGQVRNLIIASSMLVLGLGGA
                     VLNIGAITLSGTALSAIVGIILNLILPKAEKAE"
     gene            2821..3231
                     /locus_tag="U1099_001881"
     CDS             2821..3231
                     /locus_tag="U1099_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868165.1"
                     /translation="MKTRLSIKKPIIVAIALLGLVSPTVFSRGVLDYSMVLPRIGSTT
                     TGSIVKVTHSRGVNRNDSTGGGKTLNTSIRNYHSGSDITPTYSLPSGARVLMSYHGSG
                     SHYVNETTTLGLATQFGNTVRVQTTGSWSPDEEQ"
     gene            3316..>4070
                     /locus_tag="U1099_001882"
     CDS             3316..>4070
                     /locus_tag="U1099_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868166.1"
                     /translation="MKFLLMNVRRTVFSKKMLLSFLLAFSCILIGNMVAFNGIYKLEG
                     LSLFFAGSTIRGFSPISLVAAVISAIPIADRVIEDSKNHFLRLQLQRTSRIKYIWTLL
                     VTAGISGFLSLFLPYFLLLVANLCLTPYKEIYIGDYQGVFKSIFDSNQLVYSILITIW
                     YGIFGSVFAVFGLASSLAFRQKIVGVFFPCLYMILGGLFFALLDLSFLEPVGIISWGY
                     QFQLNFLLVFLHLLCIFTICLGMILYHFQFRVE"
BASE COUNT         1092 a          728 c          891 g         1359 t
ORIGIN      
        1 attggaagtc ttcttctttt tggggtaagt aaaaaatgct aagggtgtac agttgtcgta
       61 cctgaaattc tagcccctgc ttgaccgtac ttggtataac atattacaat atatacaaga
      121 acctgtgttc acagttcatc gctttcattg gagtgtttga aggtggagat aaggaaagga
      181 gattcctgac aaattaggtg gtaggcgaaa actgccacag gagcttttta ctcgtcgaag
      241 caataaagtg aaactaaatg gtgatagagt caagtattcc ttcaaaaaat tgccttgatg
      301 agcaaaaacg atttcttgca taagaacact ttgtttatga atataggttc taagatttga
      361 ccaagagagg ttattatatg ttgaaatata tccgtgccct tccccttgtt atagtagcgg
      421 gagcggttgt ttactttact atttttatct gtattgataa tggtgattgg acctatgttg
      481 tgactagagc gctagggctt aaggaagaac atgccaataa tgtttctctg attgtaattg
      541 gtacccaatc actttttcta acttcggatt ggttttacaa gcggattcgt gcctttctca
      601 atcgtcgttt ggaaaaggcc aaagggctta atcggcagat ttatctgggc tataaggcag
      661 ggattgagtt tttgatggcg ttacccaaac gaactatttt attcacattc tatctacttt
      721 tagtagttgc tgaaagtgtt ggtctgattg aaaaagccaa ggactatgct gcggtaatca
      781 tcttcataat tgctgttgac cgcgtgacca aaatctggcc ggaggaaaag cggagaatga
      841 ttgagtttag ccagaagatt aagaaacgga ttaagagtgt gggataagtc aagattaaat
      901 attttaagaa gacagctatg tcttcttttt ttgttaaaaa aaccaaacaa aatgcaatat
      961 atatattaca aaatagaaaa tatagtgtgt aataaaatta taaaaatgca caggaagatg
     1021 gctatggcat gaaaaatctt cgtttaaagt tagcgcgtgt ggaaaaagag ttggaagaag
     1081 aaatggagta ataataagca gttcagcttc ctgctctgcc attttcctaa aaaataaaaa
     1141 tttacaaaat tccttgccaa aagtaagggc ttatgttaca ataccaaaag cgtaggtttt
     1201 taaactcatc ccgtgaggtg agaaagacaa caggataatt cattaagggc ctatgactat
     1261 gtatatttag aaagtctggg tctttttgtg tacccagaat gggagattta tgagtacaaa
     1321 agccaatctt ttgtttgatg tccatgagaa accagcaccg cttcaaggga ttttgttgag
     1381 tttccagcac gtgttcgcta tgtttggtgc gacgattttg gtgccactga ttctcggtat
     1441 gcctgtatcg gttgccctct tcgcatcagg tgttgggacc ttgatttatc aagtggcaac
     1501 ccagtttaaa gttccagttt acctgggttc atcctttgcc tacatttctg ctatggcgct
     1561 tgctatcaag gaaatgggtg gtgatgtgtc ggcagctcag actggtattc tcttcgtcgg
     1621 cttgatttac gtgttgatcg ctgcacttgt caaagttatc ggtactaagt ggattgacag
     1681 cctcttgcca ccgattgtca tcggtccaat gattatcgtc atcggtctgg gtcttgccaa
     1741 ctctgccgtg acttcagcag gcttcgtcgc agacggcgac tggaaaaacg tcgtcgtggc
     1801 gattgcgacc ttcttgattg cggcttttgt caataccaaa gggaaaggct tcgccaagat
     1861 tgttcctttc ttgattgcca ttatcggtgg ttatgtgatt gccatcttcc ttggcctggt
     1921 taactttaca cccgttctgg aagcggcttg gtttgaatta ccaggtttct acctaccatt
     1981 tgaaacaggt gccttcaagt cgtacaattt ctacttcggt ccagaaatga ttgctatctt
     2041 accaattgct gttgtaacag tagctgagca tatcggtgac cacaccgttc tcagccaaat
     2101 ctgcggccgc cagttcttga aagatccagg cctcagccgt accttgattg gagatggtgt
     2161 agcgactgct gtatctgcct ttatcggtgg accagccaat acgacctacg gtgaaaatac
     2221 aggggttatc ggtatgactc gcatcgcatc agtatccgtt atccgcaatg cagccttgat
     2281 tgccattgcc ttctcattct tgggtaaatt tacagccctt atctcaacta ttccaagtgc
     2341 cgttcttggt ggtatgtcta tcttacttta cggtgttatc gcctcaaacg gtttgaaagt
     2401 tttgattgaa agccgtgttg actttggtca agttcgcaac ctgattattg caagctcaat
     2461 gctggtattg ggccttggtg gagcagttct caacatcggt gccatcaccc tttcaggtac
     2521 agccctctca gccattgtag gtattatcct caatcttatc ttgccaaaag cagaaaaggc
     2581 tgagtaaaat tgaaaataac agcaagtcag ccccggctgg ctttttttga tggtgtagag
     2641 gagattgggg tgaaataatt agttttcgag gagtgtgaaa ggtttagttg tttaattctc
     2701 ctgtcatatt tcctaagttt atttaattat aaccttgata ttttttacca tacttgatat
     2761 aatttacata tcaatataaa caacttcctt ataaggtttt catgaaagga gaagattcac
     2821 atgaaaacga gattatctat aaaaaagcca attatcgtag caatagcatt attgggatta
     2881 gtttctccga cggttttttc aagaggagta ctagattatt ccatggtctt accacgtata
     2941 ggatctacaa caaccggtag catagtcaag gtgactcatt cgcgaggagt gaatcgcaat
     3001 gattctacag gtggtggaaa gactttaaat acctcaatac gaaattacca cagcggtagt
     3061 gatataactc cgacctattc tcttccatct ggtgctaggg ttctcatgtc ttatcatgga
     3121 agtggtagtc attatgtcaa tgaaacgact actttgggat tagctactca attcggaaat
     3181 acagttagag tacaaacgac tggcagttgg agtccagatg aagaacagtg attagtaatg
     3241 agctaggaag ttgtttattg agactattga gctagtggaa tttattcacc tagctctttt
     3301 tactaggagg taaaaatgaa atttttacta atgaatgtta gaagaactgt tttctctaaa
     3361 aagatgcttt tgtcttttct cttggcattt tcctgcattt tgatagggaa tatggttgct
     3421 tttaatggca tatacaaact tgaaggattg tccctgtttt ttgcaggatc gactatcagg
     3481 ggtttttcgc cgataagcct tgtggcagca gttatcagtg cgattccgat agctgatcgg
     3541 gttattgagg atagtaagaa tcactttctg cgcctgcaat tacaaagaac atcccgtata
     3601 aaatatattt ggactcttct tgtgacggca ggaatttcag gatttttgtc ccttttttta
     3661 ccctatttcc tgcttttggt tgcaaatcta tgtctgacgc catataagga aatatatatt
     3721 ggtgactatc aaggtgtttt taagtctatt tttgatagta atcagttagt ttattctatc
     3781 ttaataacga tatggtatgg gatttttggt agcgtttttg cagtatttgg cctagctagt
     3841 tctcttgctt ttcgtcaaaa aattgttggg gtatttttcc cttgcctcta tatgattttg
     3901 ggcgggttgt tttttgcctt gctagatttg tcttttttag agcctgttgg gattatttct
     3961 tggggttacc agtttcaatt aaattttcta cttgtatttt tgcatctact gtgtattttt
     4021 accatctgtc ttggaatgat tttatatcat tttcagttcc gagtggagga
//
LOCUS       DASFSW010000152         3997 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.153, whole genome shotgun sequence.
ACCESSION   DASFSW010000152 DASFSW010000000
VERSION     DASFSW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.153"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            86..1111
                     /gene="adhP"
                     /locus_tag="U1099_001883"
     CDS             86..1111
                     /gene="adhP"
                     /locus_tag="U1099_001883"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEM4868167.1"
                     /translation="MKAVVVNPESTGVVVVDKELRSLEAGEALVQIEYCGVCHTDLHV
                     ANGDFGKVPGRVLGHEGIGIVTEIAPGVTSLKVGDRVSVAWFFQGCGMCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPEGLDPAQASSITCAGVTCYKAIKEAHL
                     EPGQWIAIYGAGGLGNLAVQYAKKVFNAHVIAVDINNNKLELAKEVGADVTINGLEVE
                     DVPGYIKEITGGGVHSTVVTAVSKVAFNQAIDSVRAGGYVVAVGLPSEYMDLSIVKTV
                     LDGIKVVGSLVGTRKDLEEAFHFGAMGLVVPVVQKRPVKDAEAVFNEMVAGTIQGRMV
                     LDFCHSH"
     gene            complement(1192..2475)
                     /locus_tag="U1099_001884"
     CDS             complement(1192..2475)
                     /locus_tag="U1099_001884"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEM4868168.1"
                     /translation="MKLQKPKGTQDLLPQDSAKWQYVENFTRSIFKQYNYAEIRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGERHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PAKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSNNPATDVETIAMAYHFFEELGIKD
                     IRLHLNSLGNPESRAAYRQALIDYLTPLKDQLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDYLDEESTAHFEAVKTMLDSLGIAYVIDTNMVRGLDYYNHTIFEFMTEVG
                     GNDLTICAGGRYDGLVTYFGGPETPAFGFGMGIERLILVLEKQGIELPLDTQLDVYIA
                     VLGQEANGGALDLVQAIRKQGFRAERDYLDRKLKAQFKSADVFGAKAIITLGGSEIES
                     GQVVVKNNQTRSQVETSLEALKTDFASILEELEKQ"
     gene            2751..2936
                     /gene="rpmF"
                     /locus_tag="U1099_001885"
     CDS             2751..2936
                     /gene="rpmF"
                     /locus_tag="U1099_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEM4868169.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVAAPTVKFDETTGDYSRSHRVSL
                     KGYYKGRRKIAKAASAE"
     gene            2952..3101
                     /gene="rpmG"
                     /locus_tag="U1099_001886"
     CDS             2952..3101
                     /gene="rpmG"
                     /locus_tag="U1099_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM4868170.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVI
                     FTEVK"
     gene            complement(3572..3997)
                     /locus_tag="U1099_001887"
                     /pseudo
     CDS             complement(3572..3997)
                     /locus_tag="U1099_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921855.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT         1108 a          863 c          841 g         1185 t
ORIGIN      
        1 atattgtgat attcttaaca atataaaaca actatattaa cacatccaaa aatgtatata
       61 aaatattaaa acaggaggtc tgcttatgaa agcagtcgtt gtaaatccag aatctactgg
      121 tgttgtcgtt gtcgataagg aacttcgttc actcgaagct ggtgaggctt tggtccaaat
      181 tgagtactgt ggtgtttgtc acactgacct ccacgttgcc aatggtgatt ttggtaaggt
      241 ccctggtcgc gttcttggtc acgaaggaat cggtattgtt acagaaatcg cacctggtgt
      301 taccagtcta aaagttggtg accgtgtcag cgttgcttgg ttcttccaag gctgtggtat
      361 gtgtgaatat tgtacaaccg gtcgcgaaac tctctgccgt accgtaaaaa atgccggcta
      421 ctctgttgac ggtggtatgg ctgagcaatg tattgtaaca gcggactatg cagtaaaagt
      481 tccagaaggt cttgatccag ctcaagcaag ttctatcact tgtgctggcg ttacttgcta
      541 caaagcgatt aaagaagctc acttggaacc aggtcaatgg attgctatct atggtgctgg
      601 tggacttgga aaccttgctg tacaatacgc taaaaaagtc ttcaacgctc atgttatcgc
      661 cgtagacatc aataataata aattggaact cgccaaagaa gttggtgcag atgtgacgat
      721 caacggactt gaagtagaag atgttcctgg atatattaaa gaaattactg gtggcggtgt
      781 acactctact gttgtaacag ctgtttccaa agtagccttc aaccaagcta ttgacagtgt
      841 tcgtgctggt ggctatgtgg tggctgtcgg acttccatct gaatacatgg acctcagtat
      901 cgtgaagact gtgctagacg gtatcaaagt ggtcggctca ctagttggta ctcgtaaaga
      961 tttagaagag gccttccact tcggtgctat gggactagtt gttccagtcg ttcaaaaacg
     1021 ccctgtcaaa gatgccgaag cagtcttcaa tgaaatggtt gccggtacta tccaaggtcg
     1081 tatggtctta gacttctgcc actcacacta atgcgttctc cactattttc aagaagtgat
     1141 tatccaatcg cttctttttt gctttctaaa ataaaaagaa gcagctttag actactgctt
     1201 ctccaattct tctaaaatac ttgcaaaatc tgttttaagt gcttccaagc ttgtttcaac
     1261 ttgacttctt gtctggttat ttttgaccac aacctgtcca gattcgattt cgctaccacc
     1321 cagggtaatg attgccttgg ctccaaagac atctgctgac ttaaactggg ccttgagctt
     1381 gcggtccaga tagtcacgtt ctgcacggaa accttgcttg cgaatggctt gaacaagatc
     1441 aagcgctccg ccattggcct cctgacccaa aaccgcgatg taaacatcta actgggtgtc
     1501 gagagggagc tcgatgcctt gcttttcaag gactaggatg aggcgctcaa tgcccatgcc
     1561 aaagccaaaa gctggcgttt ctggtccacc aaagtaggtc accaagccat cataacggcc
     1621 accggcacag atggtcaggt cattgccacc tacctcggtc ataaactcga aaatagtatg
     1681 gttatagtag tccaaaccac gcaccatgtt ggtgtcaatc acataagcaa tgcctaagct
     1741 gtccaacata gtcttaaccg cttcaaagtg agcagtactt tcttcatcta gatagtccaa
     1801 gatggacggt gcattttcca cagcaacctt gtcttctttt tccttggaat ccaacacacg
     1861 caaaggattt tcttctagac ggcgctggct atccttggaa agctggtcct tgagcggcgt
     1921 cagatagtca atcaaagcct ggcgataagc agcacggctc tcgggattgc ccagactgtt
     1981 gaggtggagg cgaatgtcct tgatccccaa ctcttcaaag aagtgatagg ccatagcaat
     2041 ggtttctacg tctgtcgctg ggttattaga accaaaacac tccacaccaa tctggtggaa
     2101 ctgacggaga cgacctgcct gcggacgctc atagcggaac attggaccca tgtaatagaa
     2161 cttagctggc ttttgcactt ctggagcaaa gagcttattt tctacataag aacgaacaac
     2221 gggtgccgtc ccttctggac ggagggtgat atgacgctct cccttgtcat agaaatcgta
     2281 catctccttg gtcacaatgt ctgtcgtatc cccaaccgaa cggctgatca cctcgtaatg
     2341 ctcgaagatc ggtgtgcgaa tctcagcgta attgtactgt ttgaaaatgc tgcgggtaaa
     2401 gttttccacg tactgccatt tggcagaatc ctgcggtagt aaatcctgtg ttccctttgg
     2461 tttttgtagt ttcataataa agtgtataga gccaacttat atcgctcgaa gttgtgcttc
     2521 ttcctttcca aattaagtca taagggctca aactctacca gtcgagcctt gcagtcctcc
     2581 ttattttacc ataaatgctc tgctattttc caaaaaagtg aaaaaaatct gtcaagtaac
     2641 tttactttac aaaaaacttt tgttatactg ttaaagttgg cgaaagtcat acgcactatt
     2701 ttttgattta taaggggctg agccctgaca tttaatggag gaagaaagaa atggcagtac
     2761 ctgcacgtcg cacttcaaaa gcgaagaaaa acaaacgtcg tactcactat aaagtagcag
     2821 ctccaactgt gaaatttgat gaaactactg gagattactc acgttcacac cgtgtatctt
     2881 tgaaaggata ctacaaagga cgacgtaaaa ttgccaaggc tgcttcagct gaataataga
     2941 agggagatac catgcgcgta aatattacac ttgaacacaa agaatctggt gagcgtttgt
     3001 accttacttc aaaaaacaaa cgtaacactc cagaccgtct tcaattgaaa aaatactcac
     3061 caaaattgcg taagcacgta atttttactg aggtaaaata agacaacata cagaaagcct
     3121 atgaaatcaa cgtttcaaag gctttttctt ttgtttttaa caagataact gaattttaac
     3181 tgattaaaat aaatcaaata attatttctg ctaaatctag catagcctgt tcaatcttat
     3241 gttttctttt ttgtattaca tgaagataga ttttcactat ctccttacca tcctcagaat
     3301 gtcctacaaa ttctcgaata acattcaaac tagtattgat aaaacaaaat atagaaccaa
     3361 ctccttcata tcattgcaag cgataaattt caatcttgtc tttgtgtctt gaccttacaa
     3421 gaaaagcacc tcaaaaatta gagttaacct ctaacttttg gggtgcagtt cagattttgg
     3481 aacgttttct atttacaact gttctaaatt ttcttagtcc atggtagata agccttattg
     3541 gcgttccaaa aaccataata ttggtgtgaa atcattcctc ttctctttgc ctgtgttgtt
     3601 tgaaaagata caaacttgat gcaagggtag ctagagcacc gcctagccat actagggtgt
     3661 tgctcgtttc acccgtattt ggtaaagtct cattttgtat tcctaccttt ggagccattg
     3721 aaactccaga ttccgttttc ttaacttcgg attgaagcac ctccgaatga ataatattgg
     3781 gtttattaac ctcaggttga cccttttctg gttggattcc ggcgggtttc tcaacttctg
     3841 ggtgcgcaac ttccggagat tttggcgtct taacgtctgg aatttttgag gttggtggtg
     3901 ttactttcgg aacctcaact tctggggctt ttggggtcgg tggaattact ttcggaacct
     3961 caacgcccgg agtttttggt ggcagaggcg cctattt
//
LOCUS       DASFSW010000153         3994 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.154, whole genome shotgun sequence.
ACCESSION   DASFSW010000153 DASFSW010000000
VERSION     DASFSW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.154"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            173..811
                     /locus_tag="U1099_001888"
     CDS             173..811
                     /locus_tag="U1099_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM4868171.1"
                     /translation="MLNKVKARFLIGVGGLIAVSFMVMIGYTIGSQSVSKQTENQIRA
                     EANKLVSKKKQEERATVLSDELVKEFLTQYYTKVRLGENNSRIKPYMTDSAFSEEEAF
                     QNKAINQVYKDYMLNYRFESASIYVNTESNVALAEVTYQVTYVSDLSEQQQRTTQTET
                     KTIMLSYSKVSDKLLVNQLTIWNGKLEDMKEATDGANSSIPTIQGTTTSENN"
     gene            850..1926
                     /locus_tag="U1099_001889"
     CDS             850..1926
                     /locus_tag="U1099_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM4868172.1"
                     /translation="MTRIKAVKQKAILNVAESLGYSFRRLSGHIYEHPDHDSFRIFAD
                     TNTFKWFSRDIQGDVIDLVQLVAGVSFKEAVSYLETGDFELTKVIEEVYQPFQYYLRE
                     EPFQQARTYLNEVRGLSDETINTFGRQGLLAQATYHSESVLVFKSFDHNGILQAASLQ
                     GLVKNEEKHDRGYLKKIMKGSHGHVGISFDIGNPKRLIFCESVIDMMSYYQLHQKQLS
                     DVRLVSMEGLKLSVIAYQTLRLAAEEQGKLAFLDTVKPSRLSHYLQAIQETTTFFQTH
                     SNVITMAVDNDEAGREFYQKLSDKGLPIFQDLPPLQRLETKSDWNDIVKQQSELSLSD
                     FIQTAQAQVNKNHPPPKWKHALEL"
     gene            1980..2207
                     /locus_tag="U1099_001890"
     CDS             1980..2207
                     /locus_tag="U1099_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868173.1"
                     /translation="MSVIERLAEKVARQEEKVSRETEKLEHYRNQLQTAMYSTFIKRQ
                     QSSQLSFHEALEQAFGKETTLQPNHRNEDTE"
     gene            2204..2593
                     /locus_tag="U1099_001891"
     CDS             2204..2593
                     /locus_tag="U1099_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEM4868174.1"
                     /translation="MSKTWNFDQPLDDVKPTSSHEERAKIAALFHKQDEKPIEEVDYV
                     AAFKQQQKESESKDVQTLASTVKQSQPKKVNITSDYKQHLADTIAQNNKDISACQKQI
                     EELHQLIDEKKIQNKKLQAISVAIDDL"
     gene            2643..2933
                     /locus_tag="U1099_001892"
     CDS             2643..2933
                     /locus_tag="U1099_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868175.1"
                     /translation="MKFFQKKKQNQDQFKRLIHRLSEMSDSELTKVEQLLDVIFDTNF
                     KPDRNVELSQSIITDEEQILDKSIQEAKNKLNTEQLEKRIEEFKQSKKPKNG"
     gene            3003..3469
                     /locus_tag="U1099_001893"
                     /pseudo
     CDS             3003..3469
                     /locus_tag="U1099_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3469..>3994
                     /locus_tag="U1099_001894"
     CDS             3469..>3994
                     /locus_tag="U1099_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM4868176.1"
                     /translation="MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAG
                     KTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQEKYGKDSVDYTKGFAGKMVEQLV
                     DELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
                     LYAIDPTQARATPKE"
BASE COUNT         1396 a          722 c          821 g         1055 t
ORIGIN      
        1 caggattttt tattgctttg aatctatttt tagtttttag tattattatg aagtggtgga
       61 gaaatagaat agtttaacac ggaggattac caatgggtag tcctttttct tgtgataaaa
      121 tctccaagct aatcttgata tgatgtgcct atcactaata aaggagtaag ctatgttaaa
      181 taaagtgaaa gctcgatttc ttattggagt aggtggctta attgcagtca gttttatggt
      241 catgattggc tacacgattg gctcacaatc cgtttcaaaa caaacagaga accaaatacg
      301 agcagaagcc aataaacttg tgtcaaagaa aaagcaggag gaaagagcga cagtcctatc
      361 agatgagctt gtcaaagaat ttctcactca atattataca aaggtccggc taggagaaaa
      421 taacagtcgt atcaaaccct acatgacgga ttcggcattt tcagaagagg aagcatttca
      481 gaataaagcg atcaatcaag tttataaaga ttatatgctt aactaccgat ttgaatcagc
      541 cagtatctat gtcaacacag aaagcaatgt tgcacttgcg gaagttacct atcaagtgac
      601 ctatgtttct gatttgagtg agcaacaaca gcgaaccact cagacagaaa ccaagacgat
      661 tatgttatcc tactccaaag tttccgacaa gctcctagtt aatcagttga ccatttggaa
      721 tgggaaactg gaggacatga aagaagcaac agatggagcc aattccagca taccaacgat
      781 ccaaggaact acaacaagcg agaacaacta gcaggagaca gtcttctgct tttttgatag
      841 ggagacaaga tgacacgaat taaagcagta aaacaaaagg ccattttaaa tgtggctgaa
      901 agtctgggtt attccttcag acgtttatca ggacacattt atgaacaccc agaccatgat
      961 tcctttcgga tttttgcgga taccaatact ttcaaatggt tttcaagaga tatacaaggg
     1021 gatgtaattg acttggttca attagtggca ggtgtttctt tcaaagaggc tgtgtcttat
     1081 cttgaaactg gagattttga actgaccaaa gtaatagaag aggtttatca accgtttcaa
     1141 tattatttgc gtgaagaacc ctttcagcaa gcacgtactt acttgaatga agttcgtggc
     1201 ctaagtgatg agactataaa tacctttggt agacaaggat tgcttgctca agctacttat
     1261 cattcggagt cagtattagt gtttaaaagc tttgaccata atgggatctt acaggccgca
     1321 agccttcaag gtctcgtcaa aaatgaagaa aaacacgatc gaggttatct caaaaaaatc
     1381 atgaaaggat ctcatggcca tgtcggtatt agtttcgata ttgggaatcc taagcgactc
     1441 attttttgtg aatcagttat cgatatgatg agttattatc aacttcacca aaagcagcta
     1501 tcagatgttc gcctggtgtc aatggaaggc ctaaaacttt cagtgattgc ttatcagact
     1561 ttgcgtctag cagcagagga acaggggaaa ttggcatttc tagatacagt aaaaccaagt
     1621 agacttagcc attatcttca ggcaatacaa gagacgacaa ccttttttca aactcattca
     1681 aacgtcataa caatggctgt tgataacgat gaagcaggaa gagaatttta tcagaaactg
     1741 tcagataaag gacttccgat ttttcaagat ttaccaccat tgcaaagact tgaaacaaag
     1801 tcagattgga atgatattgt gaaacagcag agtgaactat ctttaagtga ttttatccaa
     1861 acagcccaag cacaagtcaa taagaatcat cctccaccta aatggaaaca tgcattggaa
     1921 ttgtgataac aaaatcaagt ccgctatcat aatctagaaa gtgacaagga ggacacccta
     1981 tgagcgtgat tgaacgtctg gctgaaaaag tagctagaca agaagaaaaa gtctcacgtg
     2041 agactgagaa attggaacac tatcgaaacc aactacaaac agctatgtac agtaccttta
     2101 tcaaacggca acaatcaagt cagttgtcat ttcatgaagc actagagcaa gcctttggta
     2161 aagaaaccac actacaacca aatcacagaa atgaggatac agaatgagta aaacatggaa
     2221 ttttgatcag ccactagacg atgtgaaacc aacatcgtcc catgaagaac gagctaaaat
     2281 cgcagcactt tttcataaac aggatgaaaa accaattgaa gaagtagatt atgtggctgc
     2341 ttttaaacag caacaaaagg agtcagaatc taaagatgta caaacgctcg catcaaccgt
     2401 taaacaaagt cagccgaaga aagtaaatat cacaagtgac tataaacaac acttggctga
     2461 taccattgca caaaacaaca aggatatttc cgcctgtcag aaacaaattg aagaacttca
     2521 tcaattgatt gatgaaaaga aaatccaaaa taaaaagttg caggctattt cagtagccat
     2581 tgatgattta taagtcaggt agtcctatgc gggctacctt tttacaattg aaggagagga
     2641 taatgaaatt ttttcaaaag aaaaaacaga accaggatca atttaaacga ctgattcatc
     2701 gactatcaga gatgtcagat agtgaactaa ccaaagtaga acaactccta gatgtgattt
     2761 ttgatacaaa ttttaagcct gataggaacg ttgaattatc tcaaagcatt ataaccgatg
     2821 aggaacaaat tcttgataag tcaatccaag aagcgaaaaa taaactgaat actgagcagt
     2881 tggagaagcg aatagaggag tttaagcaat cgaagaaacc taaaaacgga taatgcacca
     2941 aatatggtgc gttatccgtt ttttatggta taatgaaagc aaatatgagg aggtctttat
     3001 ctatgatcgg agacaatata aaatcactac gccgaacaca cgatttaaca cagccagaat
     3061 ttgcgaaaat ggttgggatt tcacgcaata gcctgagtcg ctacgaaaat ggtaccagca
     3121 ctgtttcaac tgaactcatc gaccgtattt gtcagaaatt taacgtttct tatgtcgata
     3181 ttgtaggaga ggacaagatg ttaacacctg tcgaagatta ccaaaagtag aagtgataaa
     3241 agaacgtgga gcagccattt tatcacagct ttatagatac caggatagtc aagatattgt
     3301 ttttgatgat gaatctaatc cttggatttt gatgagtgat gacttggccg aattgatcaa
     3361 tacgaaaatt taccttgtcg ctactttcga tgaaatagag cgttacaatg gctatttaga
     3421 tggtatagag cggatgttag acatggtgca tcatcgggtg gtggcttaat gagactggaa
     3481 gaatttagtg aggctgagtt tcagaaggcc ttgcagcgaa ccattcgggc cttaacccga
     3541 ggaaagacga ttccagatca accgaaagct atcttgcttg gtggacaaag cggagcaggt
     3601 aagacgacta ttcatcgtat caagcaaaag gaatttcaag gtaatatcat tattatcgat
     3661 ggtgacagct accgttctca gcatcccaat tacttagccc tgcaagaaaa gtatggcaag
     3721 gatagcgtgg actataccaa gggatttgca ggaaaaatgg tagaacagtt ggttgatgaa
     3781 ctcagtacgc aaggctacca tttgctgatt gagggaacct tgcgaactac tcaagttcct
     3841 cgtcagactg ctcaattatt atcttctaaa ggttaccaag tttccttagc agtgattggt
     3901 accaaaccag agctttctta tctcagcacc ttgattcgtt acgaagagct ttatgccatc
     3961 gatccgactc aggctagagc aacaccaaaa gaac
//
LOCUS       DASFSW010000154         3985 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.155, whole genome shotgun sequence.
ACCESSION   DASFSW010000154 DASFSW010000000
VERSION     DASFSW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.155"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(167..397)
                     /locus_tag="U1099_001895"
     CDS             complement(167..397)
                     /locus_tag="U1099_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEM4868177.1"
                     /translation="MESHLVRIINRLEAMANDGGTLKRNFERDGIVVAEVAYSYDEEN
                     GSVFTLRDVAARETYAFDSIDLIAMEIYELLY"
     gene            621..2849
                     /locus_tag="U1099_001896"
     CDS             621..2849
                     /locus_tag="U1099_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEM4868178.1"
                     /translation="MLCKNCNINDATIHLYNNLNGKQQQVDLCHNCYQIMKTDPNNAI
                     LRGLGDLTNPNNMDPFSEFFNHLGGYPGNTPAGKNREQTPPTQAGEHNGRGGQTPPPQ
                     QPQQPNGLLEEFGINVTEIARRGDIDPVIGRDQEITRVIEILNRRTKNNPVLIGEPGV
                     GKTAVVEGLAQKIVDGDVPQKLRDKEVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRN
                     RREIILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGEMQLVGATTLNEYRIIEKDA
                     ALERRMQPVKVEEPSVEETITILKGIQNKYQDYHHVKYSDAAIEAAAVLSNRYIQDRF
                     LPDKAIDLLDEAGSKMNLTLNFIDPKEIDQRLIDAENRKAQATRDEDYEKAAYFRDQI
                     AKYKEMQKATISEEDIPLITEKEIEAIVEQKTNIPVGDLKEKEQSQLVNLASDLKAHV
                     IGQDEAVDKIAKAIRRNRVGLGAPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSADS
                     MIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEVEKAHPDV
                     MHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKVEASVGFGAAMEGRTQSVLG
                     QLSNFFTPEFMNRFDGIIEFQPLSKENLLEIVSLMLDDVNKRLSHNGISLHVTDKVKE
                     KLVDLGYDPKMGARPLRRTIQDQIEDAITDFYLEHPAEKDLRAVMSSKGTIQIKAQTK
                     TK"
     gene            3035..3496
                     /locus_tag="U1099_001897"
     CDS             3035..3496
                     /locus_tag="U1099_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM4868179.1"
                     /translation="MEKIAVFGEKKAGVSYQSRFGVYAVIPDEKKEQIILVQAPNGAW
                     FLPGGEIEKGENHLIALERELMEELGFTAEIGKYFGQADEYFYSSHRDTHFYNPAYIY
                     EVTSYHQIGQPLEDFNHIAWFPVNEAIDKLKRGSHKWGIEQWKLQENSFNN"
     gene            3551..3859
                     /locus_tag="U1099_001898"
     CDS             3551..3859
                     /locus_tag="U1099_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEM4868180.1"
                     /translation="MKIINTTNSHPNLVQSQLANTDAFLVETYSAGNTDVIFTQAPRH
                     YELLISNKYRAVQQNEIEQIRDFFLHRKIDERTINKAAIQTIHTDRLIEMSIPIIAEV
                     "
BASE COUNT         1296 a          885 c          752 g         1052 t
ORIGIN      
        1 ttaaaataag cccaatactc aggcaaaaaa gaaaaattca ttcatcttac tccaattctg
       61 tcatttagag tctattatac catttttgaa tatttatgca atacttttgt ttataatatt
      121 caatttacac aagaaaaaag cacccttagg tgcctctcac ttcttcttag tacaatagct
      181 catagatttc cattgcaatc aagtcgatgc tgtcaaacgc gtacgtttca cgagcagcaa
      241 catcacgcaa ggtaaatact gaaccgtttt cctcgtcata gctataagcc acttctgcaa
      301 caacaatacc atcacgttcg aaattacgtt tcaacgtacc gccgtcatta gccatagctt
      361 ccagacgatt gataattctc accaaatgtg attccatatt cgttctccta atccttttta
      421 taggaatatt ttaccacata ttgccaaaaa agtcttattt tttccctatt ttcttcctat
      481 cgccctattt ttcatagaaa gtttttccaa gcgtagcagt ttcttgattt ttctatgaat
      541 cttgttataa taaagctaga actgaccttt tttgactatt taacaatagg tcacattttt
      601 tcacagaaag aggtagaatt atgctctgca aaaattgtaa tatcaacgac gcaacgatcc
      661 atctctacaa caacttaaac ggtaaacagc agcaggtaga cctctgtcac aactgctacc
      721 aaatcatgaa aacagaccct aataatgcca tcctacgtgg gttgggagat ttaacaaatc
      781 ctaacaacat ggatcccttt agtgaatttt tcaatcacct aggtggctat cctggcaata
      841 cccctgctgg aaaaaatcgc gaacagactc ctcctactca agccggtgag cataatggac
      901 gtggtggaca gacccctcct ccgcaacaac cccaacaacc aaatgggctc cttgaagaat
      961 ttggtatcaa cgttaccgaa attgcccgtc gtggggatat tgatccagta atcggtcgcg
     1021 atcaagaaat tacccgtgtc atcgaaattc tcaaccgtcg tacaaaaaac aaccctgttc
     1081 ttatcggtga gcctggtgta ggtaaaacag cggttgttga aggcttggct caaaaaattg
     1141 tcgatggcga tgtcccacag aaattacgag acaaggaagt cattcgtctg gatgttgtca
     1201 gcctcgtaca aggaacaggt atccgtggac aatttgaaga acgcatgcaa aaactcatgg
     1261 aagaaatccg taatcgtcgc gaaatcattc tttttattga cgagattcac gaaattgtcg
     1321 gtgctggctc tgcaggcgat ggtaacatgg atgcaggcaa tatcctcaaa ccagccctcg
     1381 cacgtggtga aatgcaacta gtcggtgcaa caacgctcaa cgagtaccgt attatcgaaa
     1441 aagatgccgc cctagaacgc cgtatgcaac ctgtaaaagt tgaagaacca agtgtcgaag
     1501 aaaccattac cattctcaaa ggtatccaaa ataaatacca ggattaccat catgttaaat
     1561 actccgatgc tgctatcgaa gctgccgcag tactgtccaa ccgctacatt caagaccgtt
     1621 tcctaccaga caaagctatt gaccttctgg atgaagctgg ttcaaaaatg aatttaaccc
     1681 tcaactttat tgatccaaag gaaattgacc aacgcctgat tgatgccgaa aatcggaaag
     1741 cacaagctac acgggatgag gactatgaaa aagcggccta tttccgtgac caaattgcta
     1801 agtacaagga gatgcaaaag gcaactatca gcgaggaaga tattccactc attactgaaa
     1861 aagaaattga ggccatcgtt gagcaaaaaa caaatatccc agttggtgat ttaaaagaaa
     1921 aggaacagtc ccagcttgtg aacctcgcta gtgacttaaa agcccatgtt atcggacaag
     1981 atgaagcagt tgacaagata gctaaagcca tccgccgtaa tcgtgtcggt ctcggtgctc
     2041 caaatcgtcc cattggatcc ttcctctttg tcggaccaac aggtgtcggt aaaaccgaat
     2101 tgtctaaaca attagctatc gaactcttcg gttcggcaga cagcatgatc cgcttcgaca
     2161 tgtccgaata catggaaaaa cacgctgtcg ctaaactcgt cggagctcct ccaggctatg
     2221 taggctatga ggaggctgga caattgacag aaaaagtccg tcgtaatcct tactcactca
     2281 tcctccttga cgaagttgaa aaagcccacc cagatgtcat gcacatgttc cttcaggtct
     2341 tagacgatgg tcgtctgaca gatggacaag gtcggacagt cagcttcaaa gatactatta
     2401 tcatcatgac atcaaatgct ggtactggaa aagttgaagc aagtgtcggt tttggtgcgg
     2461 ctatggaagg gcggactcaa tcagtccttg gccaacttag caatttcttc acacctgaat
     2521 ttatgaaccg atttgacggt attattgagt tccaaccact gagcaaggaa aacctacttg
     2581 aaatcgtcag cctcatgcta gacgatgtaa acaaacgcct ttctcacaat ggcatcagcc
     2641 tccatgtaac agataaagtt aaggagaaat tagtggacct tggctacgat ccaaagatgg
     2701 gagctcgtcc actgcgcaga accatccaag atcagattga agatgctatc accgacttct
     2761 acttagaaca tccagcagaa aaagacctac gtgctgtcat gtcaagtaaa ggtacaattc
     2821 aaatcaaggc acaaacaaag acaaaataag aaaatagagc tgctccctac gagtagccta
     2881 ttttactgtt ttgctactct atttttctaa attcttcccc atctccatcc tataaaaaaa
     2941 ctttactttg taaagcctat cacaaggaaa aataataaaa aagtggtata atgtatacaa
     3001 ctcattccaa taatgaaaga ggatagaatt gcctatggag aaaattgctg tatttggaga
     3061 aaagaaggct ggcgtcagct accagagtcg ctttggtgtt tacgcagtta tcccagatga
     3121 aaaaaaagaa cagattatac tcgttcaagc accaaacgga gcttggtttc ttcctggtgg
     3181 agaaattgaa aagggagaaa atcaccttat tgctttggag cgcgaattga tggaagagct
     3241 tggattcacc gctgaaatcg gaaaatactt tggtcaggct gacgaatact tctactctag
     3301 ccaccgcgat acccatttct ataatccagc ctacatctat gaagtgacta gctaccatca
     3361 aataggacaa ccactagaag atttcaacca tattgcttgg ttcccagtca atgaagccat
     3421 cgataaactc aaacgtggta gtcacaaatg gggcattgaa caatggaaat tgcaagaaaa
     3481 ttcattcaat aactagtaaa aacactctaa tcgtgctata atgtagccaa agagatagag
     3541 gaggttccac atgaaaatca ttaacacgac aaatagtcat cccaatcttg tccaaagcca
     3601 gttggctaat actgatgctt tcctagtaga gacttattct gcaggtaata cagacgttat
     3661 tttcacacag gcaccccgtc attatgaact cttgattagt aataaatacc gtgcagtaca
     3721 acaaaatgaa atcgagcaaa ttcgtgattt cttccttcat cggaaaattg atgaacgaac
     3781 tattaacaag gctgccattc aaacgattca cacagaccgt ttgattgaaa tgtctattcc
     3841 cattatcgca gaagtgtaag aagccagatg gcttctttca gattgaagaa aaagtccatt
     3901 ttggacaatt ttcttcaatc ttttttcttt atattgaaaa gaaaaactct cagaaacgct
     3961 gaaatatcaa tgtttccaag agttt
//
LOCUS       DASFSW010000155         3975 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.156, whole genome shotgun sequence.
ACCESSION   DASFSW010000155 DASFSW010000000
VERSION     DASFSW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.156"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1644)
                     /locus_tag="U1099_001899"
     CDS             complement(<1..1644)
                     /locus_tag="U1099_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4868181.1"
                     /translation="MGETMKKKIYWKDMRQSLLSSKGRFLSIFSLMMLGALALTGLKV
                     TAPNMEKTAQAYIASHRTMDLAVISGLGLSQADLDELETIEGATLEAGYFKDVVTNEG
                     QTAIRLFSAPQTLSTYKLVEGEMPSQKGQIALSASFKNRYKLDDTVQVTESDKDGKIL
                     TQTSFTLVGFVASAEIWDNETMGMAASGDGQLGGYGLVSQDTFKSEVYTIARIRYDDL
                     VDLPYYSQAYQDRLDQHQEDVEKLLANNDEQRLAAIQSDGQTDISKGEEEIAQAQSQL
                     EQAESELETGEEQLATGRREFARGRAKVVQTEAELRAAQAQLLKNKFELEESKKELDG
                     RKAQLEQTASFLDGYQMELETSAQQLEEAKQELDSQNAQLSQTASQISTGRASWFQAQ
                     QELDLEIANHLQEGQTLSDYPDLLARQEGLDVEKSRLDQMESAHEQANQAYLQGYDYY
                     QTKQNEYNSNVAQYQTWNQEYQAGLARYQAGLSQYEQGIAAYNKGVEDYEWGLSQLES
                     SNQLLRQEEFRLEEADKELSQAQSQFSEKKATADQEISQA"
     gene            complement(1644..2345)
                     /locus_tag="U1099_001900"
     CDS             complement(1644..2345)
                     /locus_tag="U1099_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868182.1"
                     /translation="MSYIQVKNSSKYYQMGDATIVANDGVSFEIEQGELVIILGSSGA
                     GKSTLLNILGGMDSNDEGEVWIDGVDIAKLSSHELTNYRRDDVGFVFQFYNLVANLTA
                     KENVELAAEIVKDALDAEEVLEQVGLGHRINNFPAQLSGGEQQRVAIARAVAKKPKIL
                     LCDEPTGALDYQTGKQVLQILQDMSRTQGTTVIIVTHNSSLAPIADRVIRMRDARVHS
                     VELHAEPQDIASLEY"
     gene            complement(2434..2769)
                     /locus_tag="U1099_001901"
     CDS             complement(2434..2769)
                     /locus_tag="U1099_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEM4868183.1"
                     /translation="MEKTQIIQDVLALIQDLNRAFQADKVGTADEQRFQHNLTETTRI
                     LSEAESVTNTELIAIEKFYRSTSFLVGLGDLRLSETSRQAWRAFDRYYYETIREELKL
                     YGGSAIAQV"
     gene            complement(2864..3349)
                     /gene="tpx"
                     /locus_tag="U1099_001902"
     CDS             complement(2864..3349)
                     /gene="tpx"
                     /locus_tag="U1099_001902"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEM4868184.1"
                     /translation="MTTFIGKPVTLVGPQLRVGDVAPDFVLMANDLSLKSLKDFGKQT
                     KVISVVPSIDTGICDTQTRRFNQELADRDNTVVITVSADLPFAQKRWCGAAGLEDAIT
                     LSDYYDHAFGKSYGVLMREWNLLARAVFVLNADNKITYVEYLDNVNEHPDYDAALEAV
                     K"
     gene            complement(3428..>3975)
                     /locus_tag="U1099_001903"
     CDS             complement(3428..>3975)
                     /locus_tag="U1099_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEM4868185.1"
                     /translation="KETSRQAVQALQTIGLEVVMLTGDNEKTAKAIAKEVGIEQVISQ
                     VLPDDKANQVKHLQEQGKTVAMVGDGINDAPALAQAHVGLAIGSGTDIAIESADIVLM
                     HSDILDVVKAVKLSQATMRTIKQNLFWAFAYNVIGIPVAMGLLHVFGGPLLNPMFAGA
                     AMALSSVSVVLNALRLKTYKL"
BASE COUNT         1005 a          985 c          801 g         1184 t
ORIGIN      
        1 agcttggcta atttcttgat cggccgtggc tttcttctca gaaaattggg attgagcttg
       61 acttaactct ttatcagcct cctccaaacg gaattcttcc tgacggagca gttgatttga
      121 agactctaac tgacttagtc cccattcata gtcttctact cctttattgt aggctgcaat
      181 gccttgttca tactgactca aacctgcttg ataacgggcc aaacctgcct gatattcttg
      241 attccaagtt tggtactggg ctacattgct attgtattca ttttgtttgg tttgatagta
      301 atcataacct tgtaaatagg cttggttggc ctgctcatgt gcagattcca tttggtctaa
      361 tcggcttttc tcaacatcca aaccttcctg tctagccaac aagtcaggat agtctgagag
      421 ggtttggcct tcttgcaagt gattggcaat ttcaaggtct aactcttgct gagcttgaaa
      481 ccaactagct cgccctgtgg aaatctgaga tgcggtctga ctcagttggg cattttggga
      541 atccaattct tgcttggcct cttctaactg ctgggctgac gtttctaatt ccatctgata
      601 gccgtctaga aacgaagcag tctgttctaa ttgagcctta cgcccgtcca attccttttt
      661 actttcttcc aattcaaact tattttttaa tagttgggct tgggcagctc gtaattcagc
      721 ttccgtttgg acaaccttag ccctaccacg cgcaaactct ctacgacctg tcgctaattg
      781 ctcctcccct gtttccagtt ctgactctgc ctgttcaagc tgagattgag cctgagcaat
      841 ttcctcttcc ccttttgaaa tatctgtctg tccatctgac tgaatagctg ccaatcgttg
      901 ttcatcattg ttagccaata acttttctac gtcttcttgg tgctggtcca acctgtcttg
      961 ataggcctga ctgtaatatg gtaaatcaac caaatcatca tagcgaatac gggcaatagt
     1021 gtaaacttct gatttgaagg tatcctgact aacaaggcca tagccaccta actgcccatc
     1081 accacttgca gccatgccca tggtttcatt gtcccaaatt tctgctgatg cgacaaagcc
     1141 cacaagagta aaacttgtct gtgtcaagat ttttccatcc ttatcagact ccgtcacttg
     1201 aacagtatcg tccaatttgt aacgattttt gaaagaagct gatagggcta tttgcccctt
     1261 ttgactgggc atttcccctt ctactagctt ataggtcgat agggtctggg gagctgaaaa
     1321 cagccgaatt gcagtctgac cttcattagt aaccacatct ttaaagtagc ccgcttcaag
     1381 agtagcacct tctatcgttt ctaattcgtc aaggtcagct tgactaagtc ccagacctga
     1441 aattactgcc aaatccatcg ttcgatgact ggctatatag gcttgggccg tcttttccat
     1501 attgggagct gttactttca gacctgtcaa agctagagct cctagcatca tcaggctaaa
     1561 tatggacaaa aaacgcccct tagaagaaag cagagattgt ctcatatcct tccagtagat
     1621 tttctttttc atagtttcac ccactaatat tctaggcttg caatatcctg tggttctgcg
     1681 tgtaactcaa cagagtgcac acgggcgtcc cgcatccgaa tgaccctatc tgcaatagga
     1741 gccagagagc tattgtgggt cacgataatc accgttgtcc cttgtgtgcg ggacatatct
     1801 tgtaggattt gtaagacctg cttacctgtt tggtagtcca gagcgccagt tggttcatcg
     1861 caaagcaaaa ttttaggctt ttttgcaacc gcacgggcaa tggccacacg ttgttgttca
     1921 ccgcctgaaa gctgagcagg gaaattattg attcggtgac cgagtccgac ctgttccaag
     1981 acttcctcgg catccaaggc atccttgacg atttctgctg ccagttccac attttccttg
     2041 gcggtcagat tggcaaccaa gttataaaat tgaaatacaa aacctacatc atctctacga
     2101 taattggtca attcatgact tgaaagcttg gcaatatcca caccgtcaat ccaaacctca
     2161 ccctcatcgt tgctatccat gccacctaag atattgagca gagtggattt gcccgcacca
     2221 gatgaaccca gaatgatgac cagctcaccc tgctcaatct caaaggacac accatcattg
     2281 gccacaatgg tcgcatcccc catttggtag tattttgacg aattctttac ttggatatag
     2341 gacatgatac acctcgcttc ttctcttcct aacaactgta ttcttactac ttattatagc
     2401 acaaaagagg gttttcaccc tctccttcat cttttacact tgggcaatag cagagccgcc
     2461 atagagtttg agttcttctc tgatcgtttc gtagtaatat cggtcaaatg cccgccaagc
     2521 ctgcctgctg gtttcactca atcgcaggtc gcccagtcca accaaaaaac tggtcgaacg
     2581 ataaaatttc tcaatcgcaa tcagttctgt attggtaaca gactcggcct ccgacaatat
     2641 gcgtgtcgtc tctgtcagat tatgttggaa ccgttgttca tcagctgttc ctactttatc
     2701 tgcttgaaag gcccgattca agtcttgaat gagggccaaa acatcttgaa taatctgtgt
     2761 cttttccata aaagaccctc cttgtttttg ataaatttag tatatcaaat tagacaaaaa
     2821 tattccatca aaaaacgatg cctgttccgg caccgttata tctttattta acagcctcca
     2881 aagccgcatc atagtctgga tgctcgttga cattatccaa gtattctacg taggtgattt
     2941 tattgtcggc attgaggaca aagaccgcac gcgccagcaa attccactcc cgcatgagca
     3001 caccgtagga tttaccaaag gcatggtcat agtaatcaga cagggtgatg gcatcttcca
     3061 aacctgcagc gccacaccag cgtttttggg caaaggggag gtctgctgaa accgtaataa
     3121 caaccgtatt gtcacggtca gccaattctt gattgaagcg acgagtttgg gtatcgcaaa
     3181 tccctgtatc aatagaaggc acaacactga tgaccttagt ctgcttgcca aagtctttca
     3241 ggcttttcaa gctcaagtca ttggccatga ggacaaaatc aggggctaca tctcccactc
     3301 gtaactgcgg acctaccaag gtcaccggtt ttccaataaa ggttgtcata aacatctccc
     3361 ttgcacttaa tatatacatt gtaagcgttt atgtaaaaaa ataccagttt tttgactggt
     3421 atttttgcta tagtttgtag gttttcaatc gcagagcgtt caagacgact gacactgagc
     3481 tgagggccat ggctgcacct gcaaacatag gattgagcag aggtccacca aatacatgta
     3541 acagacccat agcaactgga ataccgatca cattgtaagc aaaggcccag aagagatttt
     3601 gtttgatggt ccgcatggtt gcctggctga gtttgacagc cttaaccaca tccaaaatat
     3661 cactatgcat gaggacaatg tcggcagact caatggcaat atcagtccct gaaccaatgg
     3721 ctagacccac atgggcttga gctagagctg gggcatcgtt gatgccatcc cccaccatag
     3781 cgactgtctt gccctgttct tgtaagtgct tgacttggtt agccttgtca tctggtagga
     3841 cctgactgat cacctgttca atgccgactt ccttggcaat ggctttggca gttttttcat
     3901 tatctcctgt cagcatgaca acctctagtc ctatggtttg cagagcctgg acagcctgac
     3961 ggctggtctc tttta
//
LOCUS       DASFSW010000156         3967 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.157, whole genome shotgun sequence.
ACCESSION   DASFSW010000156 DASFSW010000000
VERSION     DASFSW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.157"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(75..1229)
                     /locus_tag="U1099_001904"
     CDS             complement(75..1229)
                     /locus_tag="U1099_001904"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEM4868186.1"
                     /translation="MNIGIDKIGFAAPDYVLDLADLAQARNVDPNKFKIGLLQSEMAV
                     APVTQDIISLGAKAAEAILTEEDKQTIDMVIVGTESSVDQSKAAAVTIHGLLGIQPFA
                     RSIEMKEACYGATAGLSLAKSHIAQFPESKVLVIASDIAKYGVASGGEPTQGAGAVAM
                     LVTANPQILVLNNDNVCQTRDIYDFWRPNYDKYPRVDGKFSTEQYTDCLTTTFDYYQQ
                     KTGKTLNDFAAMCLHIPFSKQGLKGLQAIAQDEETLSRLTERFQEAIVYNKVVGNIYT
                     GSIFLSLLSLLENSRALETGDQILFYSYGSGAVCEIFSGQLVEDYRDHLQENRLEQLN
                     QRTKLSVKEYEQVFFEEITLDETGSSLNLPEDQSPFALIKVDNHKRIYRK"
     gene            complement(1258..2442)
                     /locus_tag="U1099_001905"
     CDS             complement(1258..2442)
                     /locus_tag="U1099_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEM4868187.1"
                     /translation="MKKVAIVSAYRSAIGSFGGSLKDIEIADLGAQVLETALANKNIP
                     ADSVDEVIFGNVLSAGQGQNIARQIAIRAGIPQTASAYAVNKVCGSGLKSVLLAAQSI
                     MLGDNDVVVAGGIEIMSQAPYLSKTSRFGSKFGHISLEDSMLTDGLTDAFNDYHMGIT
                     AENVAERYHVSRAEQDAFTYSSQEKAAKAIAEGRFVDEIVPIRLKNRKGETIFATDEY
                     PRLTPIEKLATLRPSFKKEGTVTAANASGINDGCAVLVLMSDEKASELNIQPLAYIEA
                     YATSGLDPALMGLGPITASQKALQKLNKTVEDIDLFELNEAFAAQSIPVVKQLGIDPA
                     KVNVNGGAIALGHPIGASGSRILVTLIHELIKQEKELGLCSLCIGGGQGISLIVSNAQ
                     TN"
     gene            2668..3360
                     /locus_tag="U1099_001906"
     CDS             2668..3360
                     /locus_tag="U1099_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEM4868188.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGLNKAEAA
                     AEFGDEQVHIWRRSYDTLPPEMAKDHEHSAHTDRRYAHLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFELDE
                     NLNIVKEYYLEA"
     gene            3466..3849
                     /locus_tag="U1099_001907"
     CDS             3466..3849
                     /locus_tag="U1099_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM4868189.1"
                     /translation="MASKKMLHACLRVENLEASQKFYEEAFGFKETRRKDYPEHKFTL
                     VYLALEGDGFEIELTYNYDHGPYIVGDGFSHLALSSDDLEGDNAKHKELGYPTTDIKS
                     LPGSPGRYYFVTAPDGYRVEVIRAD"
BASE COUNT         1136 a          769 c          889 g         1173 t
ORIGIN      
        1 gctaactgtg aaaagtgtgc tggaaattcg atcagcctat cattcattat tccaatgaat
       61 gataggtaaa atgcttattt acgatagata cgtttgtgat tgtccacttt gataagggca
      121 aagggacttt ggtcttctgg taggttaagg gatgaaccgg tttcatcaag ggtaatttct
      181 tcaaagaaga cttgttcata ttccttaacg ctgagttttg tacgctgatt gagttgttct
      241 aaacgatttt cttgtaagtg gtcgcgataa tcttcaacga gttgaccgct gaaaatttca
      301 cagacagcgc cgctaccata gctataaaag aggatttgat cccctgtctc taacgctctg
      361 ctattttcca aaagggaaag aagagagagg aagatagagc cagtgtagat gttgccgacg
      421 actttattat agacgatagc ttcctggaaa cgttctgtca agcgactaag agtttcttca
      481 tcctgagcaa tagcttgcaa tcctttaaga ccttgtttgg agaaaggaat atgtaagcac
      541 atggcagcaa aatcgttgag ggttttgcct gttttttgtt gatagtagtc gaaagttgtc
      601 gtcaagcaat cggtgtactg ttcagttgaa aatttaccat caacacgtgg atatttgtcg
      661 tagttaggac gccagaagtc gtagatgtca cgtgtctggc agacattatc attgttgagg
      721 accaaaattt gtgggtttgc ggttaccaac atggcaactg ccccagcacc ttgggtaggt
      781 tcaccgccag aagcgactcc atactttgca atatcactag caatgactag gacttttgat
      841 tctgggaatt gagcgatatg gcttttggca agacttagtc cagccgttgc accgtaacaa
      901 gcctccttca tctcgatgga gcgagcgaag ggctgaatac cgagtaatcc atgaatggtg
      961 acggcggcag ccttactttg atctacgcta gattctgtgc cgacaataac catgtcaatg
     1021 gtttgtttat cttcttctgt cagaatggct tcggcagcct ttgccccaag actgataata
     1081 tcttgagtga caggagctac agccatttca gactgaagaa gaccaatctt aaatttattt
     1141 gggtccacat tgcgggcttg ggctaaatca gccaaatcta aaacataatc gggtgccgca
     1201 aatccgattt tatcaatacc gatgttcata atatatcctt tgtgtacaaa tgataggtta
     1261 atttgtttgc gcattggaaa cgattaggga gatgccttga ccgcctccga tacaaagtga
     1321 gcaaagacca agctcttttt cttgcttgat taattcgtga atcagagtga ctaggattcg
     1381 actaccacta gctccgattg gatgtccaag ggctatagca ccaccattga cattgacttt
     1441 tgcaggatca atgccgagtt gtttgacaac tggaattgat tgggctgcga atgcttcatt
     1501 gagttcgaat agatcgatat cctcgactgt tttattcagt ttttgtagcg ctttttggct
     1561 agcagtaata ggtccaagcc ccatcaatgc aggatctaaa ccgcttgttg cataggcctc
     1621 aatataggcg agtggctgga tatttagctc actggctttt tcatcagaca tgagtaccaa
     1681 gacagcacag ccgtcgttga tgccagaagc attggctgcg gtgacggttc cttccttttt
     1741 aaaagatggc cgaagtgttg ccaatttttc aattggcgtc agacgagggt actcatctgt
     1801 ggcaaaaata gtttcgcctt tccgattttt taatctaata ggaacaattt cgtcaacaaa
     1861 tcttccctca gcaatagctt ttgcagcctt ctcttggcta ctgtatgtaa aagcatcttg
     1921 ctctgcgcga ctgacatgat agcgttcagc aacattttct gcagtaatgc ccatgtgata
     1981 gtcgttgaag gcatctgtca aaccatctgt taacatggaa tcttccagac taatatgacc
     2041 aaatttacta ccaaaccggc tagttttgga tagataaggt gcctggctca taatttcaat
     2101 gcctccagcc acaactacat cattgtcacc tagcatgatt gactgggcag ctaggaggac
     2161 tgatttgaga ccggaaccac agactttatt gactgcatag gcagaggctg tctgaggaat
     2221 gcctgcacga atagcaattt gacgtgcaat attttgtcct tgtcctgctg aaagaacatt
     2281 tccgaagata acttcatcaa cgctgtctgc agggatgttt ttattagcta aggctgtttc
     2341 gaggacttgc gcccctaaat cagcgatttc aatatctttc agactacctc cgaaactgcc
     2401 gatggcagaa cgataggcag acacgatagc tacttttttc atgataactc ctatagtggt
     2461 atcgttttga actgtcgtgt tcagccttac tattatacca ttattttgta ttttttctaa
     2521 gaagaaagat aggtctagag actgaattta catgaaagag taatgaaagc gtacacagat
     2581 tgtggaaagg ctggcatttt tttacctttt cgtgtaaaat agagaagtat aagaggggaa
     2641 cctcaaaatt taaaggagaa tccataaatg gtaaaattgg tttttgctcg ccatggtgag
     2701 tctgaatgga acaaagctaa ccttttcact ggttgggctg atgttgattt gtctgaaaaa
     2761 ggtacacaac aagcaatcga tgcaggtaaa ttgatcaaag aagcaggtat cgaatttgac
     2821 cttgctttca catctgtatt gaaacgtgct atcaagacta caaacttggc tcttgaagct
     2881 gcagatcaat tgtgggtacc agttgaaaaa tcatggcgtt tgaacgaacg tcactacggt
     2941 ggtttgactg gcttgaacaa agctgaagca gctgctgaat ttggtgatga acaagttcac
     3001 atctggcgtc gttcttatga tactttgcca ccagaaatgg ctaaagatca tgagcattca
     3061 gcacacactg accgtcgcta tgcacacctt gatgattcag ttattccaga tgcagaaaac
     3121 ttgaaagtaa cgcttgagcg tgcccttcca ttctgggaag ataaaattgc tccagcattg
     3181 aaagacggta aaaacgtatt cgtgggtgca cacggtaact caatccgtgc ccttgtaaaa
     3241 cacatcaaac aattgtctga tgacgaaatc atggatgtgg aaattccaaa cttcccacca
     3301 cttgtattcg aattggatga aaacttgaac attgtgaaag agtactactt ggaagcataa
     3361 ttcatttaag gcctgagttc gctcaggctt ttttagtttt gaatatcgga tttttcatag
     3421 agagtatggt atactggtgg tattaaatgg taggaggaat ttattatggc aagtaaaaaa
     3481 atgttgcacg cttgtttgcg tgttgagaac ttagaagcat ctcaaaaatt ttatgaagaa
     3541 gcatttggat ttaaggaaac ccgtcgtaag gactatccag agcacaagtt taccttggtt
     3601 tacttagcac ttgaaggaga tggctttgag attgaattga cctacaatta tgatcatggt
     3661 ccctatatcg taggcgacgg tttctcgcat ctagctcttt cttcagatga tttagaagga
     3721 gacaatgcta agcataagga gctgggctat ccaacgacag acatcaagag cttgccagga
     3781 agtccaggtc gttattattt tgtgacagct ccagatggct accgtgtgga agtcattcgt
     3841 gcagattaat ttagaaaaga ttgcctaggc agtctttttc ttttcaaaaa aatcagaaaa
     3901 tggcagaagg gagggattag gaagcaaatc aattatttat acttaatgaa aatcaaaagt
     3961 agcctag
//
LOCUS       DASFSW010000157         3832 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.158, whole genome shotgun sequence.
ACCESSION   DASFSW010000157 DASFSW010000000
VERSION     DASFSW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.158"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..510
                     /locus_tag="U1099_001908"
     CDS             <1..510
                     /locus_tag="U1099_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEM4868190.1"
                     /translation="GLIPRFEKLIATIRSREISAAIILQAKSQLKAIYKDNADTIIGN
                     CDTELFLGGKEGTTLKELAENLGKETIELYNTSETRSNQKSFGLNYQKLGKELMSRDE
                     LKVMDGGKCILEIRGTRPFYSDKYDITKHKHYKLLEDFDKRNKFDIEAYLKNRGQAKL
                     TENTSIIQL"
     gene            586..894
                     /locus_tag="U1099_001909"
     CDS             586..894
                     /locus_tag="U1099_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM4868191.1"
                     /translation="MEHEMININAHLVKDVIFSSFETEDGIVPVVNFSLVKKYGKGKE
                     YTNCSAYGDKVEIAKEFEKGDLIHVFGYFKENRKGDKVYKNFIVKSMNKIEIEQMEEE
                     "
     gene            894..1109
                     /locus_tag="U1099_001910"
     CDS             894..1109
                     /locus_tag="U1099_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HEM4868192.1"
                     /translation="MDFFTQGVDVLKTLVIAIGGGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANLIQ"
     gene            1118..1981
                     /locus_tag="U1099_001911"
     CDS             1118..1981
                     /locus_tag="U1099_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868193.1"
                     /translation="MFDIFGKLEEMIQEILLDMIKGTLTSMFTDLNHQLDFVSGQVGQ
                     SPSQFNGEVFSFIKHINDSVVLPIAGLVITAVLCLELIQVVMQKNNMAEVDTFEFFKY
                     VIKMWVAVYLVSHAFEFAMAAFDVGQHMIGQATGVIHSETSISSEQITAMVDSLKEKN
                     IGELVGIVFELQVVKLTIQIISLIILIITYGRMFEIYAYSSVAALPFATLGNRDWGQL
                     GHNYIRGLFALALQGLFLVICFGIYAVLLRGVKMTDIHMSVFSILGYTVLLGFMMLKT
                     GSLARSIMNAH"
     gene            1994..2362
                     /locus_tag="U1099_001912"
     CDS             1994..2362
                     /locus_tag="U1099_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEM4868194.1"
                     /translation="MAYVQIPKDLSKVKTKIAFNLTKRQLIGFSVAGLASLPVYLSIR
                     ESVGNDLAMLVFIVLAFPFLFLTFYEKDGLTSEIYLKYIFLQQHYQPCVRYKRTLYKK
                     MHKEEVHETRKKKKTGTRVS"
     gene            2319..>3832
                     /locus_tag="U1099_001913"
     CDS             2319..>3832
                     /locus_tag="U1099_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEM4868195.1"
                     /translation="MRPERKRKQAQEFLEAKEVQLKKIQKETLKQDNNTLLGSIKSFF
                     ELGDKCLTAEDTIPIEKVYENGIFQLTDGYYTKMIAFEDINYQLALEEQRDFIFNQFA
                     AFLNSFDNSTRLQLCFTNQLGRLDELADAITIPSKDDGLEAIRCEFRVMLQNQLEKGN
                     NGLRQTKYIVFGVKEDTYRQAKVVLSRLEIDLQSHLKGMGIRSIVLNGFERLIVLHHI
                     LNPTEQVSELPEDLGNSDCRSSIVPDKFHFQKKYFSMDNVLCQTNHVQILASELSDRF
                     LTELLSLEENMVLSLHIQALDQVEAIKLVKRKFTDVQRMTMEEQKKAYRSGYDLDIIP
                     SDLMTYGTDLKHLLNDLQSRDEKMFLVSFILLNQGLTKAKLDNAVSQLATICSRHNCM
                     LKCLDYQQEQGLISALPLGMNCIKIKRQLTTSSTAVFIPFTTEELFMTSSTSIYYGLN
                     AISHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREISNAILVTDDDILICDPEG"
BASE COUNT         1274 a          563 c          790 g         1205 t
ORIGIN      
        1 ggtttaattc cgagatttga aaagttaatt gctacaattc gttctcgtga aatttctgcg
       61 gcaatcatcc ttcaagcgaa atctcaattg aaagccatat ataaggacaa tgctgacaca
      121 attattggaa actgtgatac cgagttattt cttggaggaa aagaaggtac tacgctaaaa
      181 gaattggctg aaaacttagg taaagaaact attgaactct acaatacaag tgaaaccaga
      241 tccaatcaga aatcatttgg tcttaattac cagaaactcg gaaaggagtt aatgtccaga
      301 gatgagttaa aagtgatgga tggaggcaaa tgtatcctgg agattcgtgg gacacggcca
      361 ttttattctg ataagtatga tattactaag cataagcatt acaaactttt agaggatttt
      421 gataagagaa acaagtttga tattgaggcc tatctcaaga atagaggcca agccaaactg
      481 actgagaata ctagtattat acagttgtag gaggtgcctg atgaggatta gggaaccacc
      541 aatattctat atatttattt agaatatcat agaaagagga aaattatgga acatgaaatg
      601 attaatatta atgctcatct tgtcaaagat gtcatttttt ctagctttga aactgaggac
      661 ggtatcgtac cagttgtaaa tttctcacta gtaaaaaaat acggaaaggg taaggaatac
      721 actaattgtt ctgcctatgg cgacaaggta gaaattgcaa aagaatttga aaaaggtgac
      781 ctcattcatg tgtttggtta ctttaaggaa aatcgaaaag gtgataaagt ctacaagaat
      841 tttatagtaa aatcgatgaa taaaattgaa atagaacaaa tggaggaaga gtaatggatt
      901 tttttacaca aggcgttgat gtcttaaaaa cgttggtaat tgctattggt ggtggtttag
      961 gtgcctgggg tgttatcaac ctcatggaag gttatggtaa cgataaccct ggagcaaagt
     1021 cacaaggtat taagcaacta atggctggtg gaggtattgt gttaattggt ttaaaactaa
     1081 tcccgctact tgctaactta atccagtagg aggggctatg tttgatattt ttggaaaact
     1141 agaagaaatg attcaagaaa tccttttgga tatgataaaa ggaacattga caagtatgtt
     1201 tactgacttg aatcatcaat tagattttgt atctggccaa gtaggtcaat cgcctagcca
     1261 gttcaatgga gaagtgtttt cctttatcaa acacattaat gatagtgtcg tactaccaat
     1321 cgctggattg gttattacag ctgttttatg tttagagtta atccaagtag tgatgcaaaa
     1381 gaacaatatg gcagaagttg atacgtttga atttttcaaa tatgtcatca agatgtgggt
     1441 tgcagtgtat ttagtctccc atgcttttga atttgcgatg gcagcctttg atgtaggtca
     1501 acacatgata ggacaagcaa caggagtcat tcatagtgag acatctattt catctgagca
     1561 gattactgcc atggttgaca gtttgaaaga gaagaacatt ggggaattag taggaatagt
     1621 ctttgaactt caagtagtaa aactaactat tcaaataatt tcattgatta ttctcattat
     1681 cacatatgga aggatgtttg agatatatgc ctatagttca gttgccgctt tgccatttgc
     1741 tacacttggc aatagagatt ggggtcaatt gggacataac tacattcgtg ggctgttcgc
     1801 attggcttta caaggattgt tcctggtcat ctgttttgga atttatgctg tattactaag
     1861 aggtgtaaaa atgacggata ttcatatgtc cgtcttttca attttaggtt atacagtatt
     1921 acttggattc atgatgttga aaactggttc ccttgcaagg tcaatcatga atgcccatta
     1981 gagaggagat aatatggcat atgttcaaat tccaaaggat ttatccaagg taaaaacaaa
     2041 aatagctttt aatttaacca agcgtcagtt aattggtttt tcggtggcag gcttagcaag
     2101 tctgcctgtt tatttatcta ttagggaaag tgttggtaac gatttggcta tgctcgtctt
     2161 tattgtactc gctttccctt ttttattctt aacgttttat gaaaaggatg gattaactag
     2221 tgaaatctat cttaagtata ttttcttaca acaacactat cagccatgtg tgagatataa
     2281 gagaacactc tacaaaaaga tgcacaagga ggaggtacat gagaccagaa agaaaaagaa
     2341 aacaggcaca agagtttctt gaagcaaagg aagtccaatt aaaaaagatt caaaaggaaa
     2401 ctctaaaaca ggataataac acgttacttg gttctattaa atcatttttt gaattaggag
     2461 ataaatgttt gactgctgaa gatacaatac caattgaaaa agtatatgaa aatggaattt
     2521 ttcaattaac tgatggttac tatacaaaaa tgattgcctt tgaagatatt aactatcaat
     2581 tagcattgga agagcaacga gatttcattt tcaatcagtt tgcggcattt ttaaatagtt
     2641 ttgataattc gaccagattg caactttgtt ttactaacca acttggaaga ttggatgaat
     2701 tagcagatgc aattacaatc ccatctaaag atgatggatt agaggctatt cgttgcgaat
     2761 ttagagttat gcttcaaaat caattggaga aggggaataa cggcttaagg caaacaaaat
     2821 atattgtttt tggtgtaaaa gaagatactt atcgtcaagc aaaggttgtt ttatccaggt
     2881 tggaaattga tctacaatct catttgaaag ggatggggat tcgttctatt gtcctaaatg
     2941 gttttgaacg attgattgta cttcatcata ttttgaaccc gacagagcaa gtttctgaat
     3001 tgccagagga tttgggcaat agtgactgtc gctcttcgat tgtgccagat aaatttcact
     3061 ttcagaagaa gtatttttca atggataatg ttttgtgtca gaccaatcat gtgcagattt
     3121 tagcatcaga attatcggat aggtttttaa ctgaattact ttctcttgag gagaatatgg
     3181 ttctatcgct acatatacaa gcattagatc aagttgaagc gatcaagcta gttaaacgta
     3241 agtttaccga tgtgcaacgg atgacgatgg aggagcagaa aaaggcctat cgttctggtt
     3301 atgatttaga tattattcct tccgatttga tgacctatgg tactgatttg aaacacctat
     3361 tgaatgattt gcagagtcgt gatgaaaaga tgtttctcgt atcgtttatc cttcttaatc
     3421 aaggattaac aaaagcaaaa ctggataatg cagtctctca attagcaact atttgtagcc
     3481 gacataattg tatgctgaaa tgtctggatt accaacagga gcaaggatta atatcagctt
     3541 taccgttagg aatgaattgt atcaaaatta agcggcaatt aacgacttca agtacggccg
     3601 tcttcatacc ctttacgact gaagagcttt ttatgacttc aagtacatct atctattatg
     3661 gattaaatgc tataagtcat aacttgatta tggcagatag gaagaaacta aagaacccca
     3721 atggcttaat cttagggaca ccaggttctg gaaaatcttt ctcagccaag cgtgaaattt
     3781 ccaatgccat actagtaaca gacgatgata ttcttatctg tgatccagaa gg
//
LOCUS       DASFSW010000158         3802 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.159, whole genome shotgun sequence.
ACCESSION   DASFSW010000158 DASFSW010000000
VERSION     DASFSW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.159"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            52..1380
                     /locus_tag="U1099_001914"
     CDS             52..1380
                     /locus_tag="U1099_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM4868196.1"
                     /translation="MNRLMHFLEHKLAPFGTKVGNQRHLKAVRDGFMMAMPLVLVGSF
                     FLLLISWPDQASGGFSVVGFLEQIGLLGILNRLNQSTMAIISLIATFGIAYRLSESYE
                     TDGPSAGVIALSSFILLAPRFSTIVFNESGEQINQVFAGAIPFGSLNASALFVAIAIG
                     LVSAEIYRFFIQRGITIKMPKNVPDVVGKSFAALLPALTTLIFWACVFQGLEAAGVEG
                     GLGAVLGLIVGKPLGLIAGSLGGMVLVVLVNSLFWFTGVNGGQVLNAFVDPVWLQFTE
                     ANQVAAAAGEKLPHIITLPFKDLFVFMGGGGATVGLAICLLVFSKSRTFKSLGRISFI
                     PSLFNINTAILFSFPTVLNPIMMIPFILTPTVNAIITYFAMATGLVPMTTGVVLPWTM
                     PPILGGFLATGGSVQGAILQIILIGVSFIIYYPFFKVADNKNLELEASGE"
     gene            1385..3271
                     /locus_tag="U1099_001915"
     CDS             1385..3271
                     /locus_tag="U1099_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEM4868197.1"
                     /translation="MKLTIFDKVQNKTSQFAIDELTYYLKAIFDFELGRTTVEEDANI
                     LLSKEYFDNNDTIGIEMKNGNAILSGNSDISILIAVYRLLSEFGAVFTRPGRKHELIP
                     SLSISDWNEKELSIHETASYKHRGVCIEGADSYQNIKEFLDWLPKINMNSFFIQFENP
                     YSFLKRWYEHEFNPYADKEDFNTEIAQQMSDLLDEELSKRGLIHHRVGHGWTGEVLGY
                     SSKYGWESGLILPEEKKPLVAELNGKRELFNTAPILTSLCFSNPDVGERMCELIVNYA
                     KERKDVDYLHVWLSDARNNICECEKCKEEIPSDQYVRILNQLDRKLSEEGLNTKICFL
                     LYHELLFAPKHEVLENPERFTMMFAPITRTFEMSYADVDYENNVPLPNEYTRNNIVLP
                     NSLEENLSYLFSWQKVFNGDSFVYDYPLGRAHYGDLGYMKISKVIFRDVVYLKKLGLN
                     GYISCQELRAGFPHNFPNFVMGQMLWNRNLEYDELKALYFSSLYGSEWKSVVTYLEKL
                     SDYSSCDYFNSIGDRQNAELSMKYNISSKLAFNFINILEENVAKNYGIKRDAWRQLGY
                     HREYVIKLANALYLMASGDYLDGQIQWRDFLDYIRNHESDFQPYLDVYRIIEVAKNYA
                     GFKL"
     gene            3391..>3802
                     /locus_tag="U1099_001916"
     CDS             3391..>3802
                     /locus_tag="U1099_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEM4868198.1"
                     /translation="MNKKIFLNSAIILLSVTCFTGSVDALDNSRVIAIPSEIVNSENA
                     YNITLKAKTDGVESDLGGSIVSENGVLSGSNGTKINLKITTNPGYRFVGYQSFLENGN
                     STDGLLPIENDSFEISDKTGNVTVIAIFESVPVDE"
BASE COUNT         1203 a          555 c          758 g         1286 t
ORIGIN      
        1 gcttacatga tatctttatt ttacaaaata aaattaatgg aggataagaa aatgaataga
       61 ttaatgcatt ttcttgaaca taaacttgct ccttttggaa ctaaggtagg aaatcaacgt
      121 catttgaaag cagttcgtga cggttttatg atggctatgc ctcttgtatt agttggctcg
      181 ttcttcctgt tgctaatcag ttggccggat caggcttctg ggggattttc agtagtaggc
      241 tttttagagc aaattggttt gttgggaatt ttaaatcggt taaatcaatc aacaatggcg
      301 atcatctctc tcatagctac attcggtatt gcatatcgat tatccgaatc atatgagaca
      361 gatggacctt ctgcaggtgt tattgcctta tcttctttta ttttacttgc accaagattc
      421 tcaacaatag ttttcaacga atctggtgaa caaattaatc aagtatttgc tggagcaata
      481 ccatttggaa gtttgaacgc aagtgcatta tttgttgcta ttgctattgg attagtttca
      541 gctgaaattt atcgcttctt tattcaaaga ggtattacaa ttaaaatgcc aaaaaatgtc
      601 cctgacgttg tgggtaagtc gtttgctgca ttgcttccag cattaacaac attgattttc
      661 tgggcgtgcg tcttccaggg attagaagct gccggtgttg aaggtggact tggagcggtt
      721 ttaggattaa ttgtaggtaa accactagga cttattgcag gaagtctagg tggaatggtg
      781 cttgttgtgc ttgtaaattc acttttctgg tttactggcg ttaatggtgg acaagtgtta
      841 aatgcttttg ttgatccggt ttggttacag ttcacagaag caaatcaagt tgcagcagct
      901 gcgggagaaa aattaccaca tattattact ttgccattca aagatttgtt cgtatttatg
      961 ggcggtggtg gcgctacagt aggattagcg atttgcttac ttgtctttag taaaagtcgt
     1021 acatttaaga gtttaggacg aatctccttt attccttctc tctttaatat taatactgct
     1081 attctgttca gttttccaac cgttttgaat cctattatga tgattccttt tattctcacg
     1141 ccaacagtaa atgcaataat tacctatttc gctatggcaa ctggattagt tccgatgaca
     1201 acaggggttg ttcttccttg gacaatgcct ccaattcttg gtgggttcct tgctactggc
     1261 ggtagtgttc aaggagcaat tttacaaatt attttaatag gagtctcatt cattatttat
     1321 tatccatttt tcaaagtggc cgataataag aatttagagc ttgaagcatc aggagagtga
     1381 tcatatgaaa ttaacgattt ttgataaagt acagaataaa acttctcaat ttgctataga
     1441 tgaacttaca tattacttga aagccatttt tgattttgag ttaggaagga caactgttga
     1501 ggaagatgca aatattctac tctcaaaaga atattttgac aataatgata cgattgggat
     1561 tgagatgaag aatggaaatg cgatattatc aggtaattct gatatctcta ttcttattgc
     1621 ggtatatcgc ttacttagtg aatttggagc tgtattcact agaccaggta gaaaacatga
     1681 actaattccc tcgttatcga tatctgactg gaatgagaaa gagctctcta ttcacgagac
     1741 tgccagttat aaacatcgag gagtatgtat agaaggtgca gatagttacc agaatataaa
     1801 agaatttttg gattggttac caaaaattaa tatgaatagc ttttttattc agtttgagaa
     1861 tccgtattca tttttaaagc gctggtatga acatgaattt aatccgtatg cggataaaga
     1921 agatttcaat acggaaattg cacaacaaat gagcgacctt ttagatgaag aattatcaaa
     1981 acgaggttta atacatcatc gtgttggaca tggttggact ggtgaagtac ttggatattc
     2041 atctaagtac ggatgggaat ctgggttaat actacctgaa gaaaagaaac ccctagtcgc
     2101 tgaattaaat ggaaaacgtg agttatttaa tacagcgcca atactaacta gtctatgttt
     2161 ctcaaatcca gatgttggtg aaagaatgtg tgaactaatt gtaaactatg caaaggaaag
     2221 gaaagatgta gactacttac atgtttggtt atccgatgca cggaacaata tttgtgaatg
     2281 tgaaaaatgt aaagaagaaa taccctcaga tcagtatgta cgaattttga atcaattaga
     2341 tcggaaatta agtgaagagg ggcttaatac aaagatttgt tttttacttt atcatgaatt
     2401 gctttttgca ccaaagcatg aagttttaga gaatcctgaa cgtttcacaa tgatgtttgc
     2461 gcctattacc agaactttcg aaatgagtta tgctgatgta gactatgaaa acaatgtccc
     2521 acttccaaat gagtatacaa gaaataatat tgttcttcct aactcacttg aagaaaatct
     2581 ctcatattta tttagttggc aaaaagtgtt taatggagat agttttgtct atgattaccc
     2641 acttggtcgg gcacattatg gtgatttagg atatatgaag atcagtaaag taatctttag
     2701 agatgtcgta tatcttaaaa aattgggttt aaatggttat attagttgcc aagagttacg
     2761 cgcaggtttc cctcataact tccctaactt tgtgatgggt caaatgttgt ggaacagaaa
     2821 tttagagtac gatgaattga aagcgctcta tttcagttcc ttatatggtt ctgaatggaa
     2881 aagtgttgta acgtacttag aaaaattatc agattactct tcatgtgatt attttaattc
     2941 aataggtgat cgccaaaatg ctgaattatc aatgaaatac aatatctcaa gtaaattagc
     3001 atttaatttt ataaatatct tggaagagaa tgtggctaaa aattacggaa ttaaacgtga
     3061 tgcttggcga caattaggtt atcaccgaga atatgttatc aaattggcca acgcccttta
     3121 tctaatggcc tctggagact atctcgatgg ccaaatccaa tggagagact ttttagatta
     3181 tattagaaat catgaatcag attttcaacc atatcttgat gtttaccgta tcattgaagt
     3241 tgctaaaaat tatgctggat tcaaattgta gtcctatgga ttagattaga aaaatagcca
     3301 attcactatt tatttttaag tgaattggct tgatgtctat tattagaagg ataaataaaa
     3361 atatttgatg ttattttttg agagggttac atgaataaaa aaattttttt aaactcagcg
     3421 attatattac tctctgttac ttgttttact ggttctgttg atgctttaga taatagtaga
     3481 gtgatagcga taccaagcga aatcgtaaac agcgaaaacg catacaacat tactttaaaa
     3541 gcgaaaactg atggagttga aagtgatctt ggaggaagta ttgtttctga aaatggtgtc
     3601 ttatcaggtt ctaatggcac taaaataaat ttgaagataa ccacaaatcc tggttatcga
     3661 tttgtaggtt atcaatcatt tttagaaaat ggaaatagta cggatggatt gctacctatc
     3721 gaaaatgata gttttgaaat ttcagataaa acaggcaatg tgaccgtaat agcaatattt
     3781 gagtcagttc cagttgatga at
//
LOCUS       DASFSW010000159         3771 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.160, whole genome shotgun sequence.
ACCESSION   DASFSW010000159 DASFSW010000000
VERSION     DASFSW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.160"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            115..810
                     /locus_tag="U1099_001917"
     CDS             115..810
                     /locus_tag="U1099_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEM4868199.1"
                     /translation="MKLLITSGGTSEAIDQVRAITNHASGNLGKIIAEQALKRGHEVT
                     LVTTRQAVKPDSQKNLTIIEITNVDSLKETLEPLVKSHQALIHSMAVSDYTPVYMTGL
                     DELRATEDSSSLLKKQNTESKISSKDDYQVLFLKKTPKVISYVKKWNPAITLFGFKLL
                     VNVPKEELFAVARQSIERNGADYILANDLKDIGENQHIAYLVDKTREIQAQTKDEIAQ
                     LILNTLEKGEQNG"
     gene            803..1351
                     /gene="coaC"
                     /locus_tag="U1099_001918"
     CDS             803..1351
                     /gene="coaC"
                     /locus_tag="U1099_001918"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEM4868200.1"
                     /translation="MANITLAVTGSISAYKAADLTSQLTKLGHQVTVLMSRSAMDFIT
                     PLTLQSLSKNLVHTDVMIEENPTLIKHIDIAKETDVFLVAPASANTIAKLANGMADNI
                     ITATALALPIGTKKLLAPAMNTNMYLNPATQKNIKTLIEYGFEEIKPREALLACGDFG
                     TGALAEVTDILEKVSNTLDEKK"
     gene            1338..1907
                     /locus_tag="U1099_001919"
     CDS             1338..1907
                     /locus_tag="U1099_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM4868201.1"
                     /translation="MKKNKSTQIATIAIFFAVMIVINILSSIIFNILPVPIKPTIVHI
                     PVIIASIIYGPRVGACLGALMGLMSMTHNTIILLPTSYLFSPFVENGNVYSIIIAVVP
                     RILVGITPYFVYKWIKNRKGLFIAGAIGSMTNTIFVLGGIFFLFSSVFDGNIQAMLAT
                     VLGTNSIAEMIISSVLTAALVPTLQKIQK"
     gene            1998..3716
                     /locus_tag="U1099_001920"
     CDS             1998..3716
                     /locus_tag="U1099_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEM4868202.1"
                     /translation="MTYQETYQTWLDFADLPDYLREELVAMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINVFVVRQATEGLAKLVESKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAAHGIKSYVFESLRPTPELSFAVRHYNAIAGIMVTASHNPKEFNGYKVYGEDG
                     GQMPPADADALTNFIRAIDNPFAVELADLEASKENGLITVLGEETDVKYLEELNDLNI
                     NPELIAEYGKDMKIVYTPLHGTGEMLARRALAQAGFESVQVVEAQATADPDFSTVASP
                     NPESQAAFALAEELGREVGADVLLATDPDADRVGVEVRQADGSYWNLSGNQIGAIIAK
                     YILEAHKQAGTLPANAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLMVAEIAAYYRSRGMTLADGIDEIFKE
                     YGYFAEKTISVTLSGKDGAEQIKAIMAKFRDNSPAQFNATDIAVFEDFALQTKTDKDG
                     NIEKLTTPPSDVLKYTLADDSWFAVRPSGTEPKIKFYIATVGETLAEAEEKISNIEKE
                     INEFVG"
BASE COUNT         1172 a          790 c          732 g         1077 t
ORIGIN      
        1 acaggccctt atattttttt gtattagcag catcattttc cagtgtcaca gattgattag
       61 caaatcgatt agtgcaaact ttacgaaaaa tagaaattct agtaaaatat gaacatgaaa
      121 ctattgatta catcaggcgg aacaagtgaa gctatcgatc aagtccgcgc tattacaaac
      181 catgcttctg gaaatcttgg aaaaatcatt gccgaacaag ccttgaaacg cggtcatgaa
      241 gttactcttg tgactacaag acaggctgtt aaaccagatt ctcagaagaa tttaaccatt
      301 atcgaaatca caaatgttga tagtttgaaa gaaacattag aacctttagt caaatcacat
      361 caggccctga ttcatagcat ggctgtatcc gattatacac ctgtttatat gactggttta
      421 gatgaactaa gagcaactga agacagttct agtctactaa aaaaacagaa tacagaaagt
      481 aagatttcat ccaaggatga ctaccaagtt cttttcttga aaaaaactcc caaggtcatt
      541 tcctatgtaa aaaaatggaa tccagctatc acactctttg gcttcaaact attggttaat
      601 gttcctaagg aggaattatt tgccgtagct cgccaaagca ttgaacgaaa tggcgctgat
      661 tatatacttg ctaatgactt gaaggatatt ggagaaaatc aacacattgc ctatttggtt
      721 gataaaacaa gggagattca agcacagaca aaagatgaaa ttgctcagct cattttaaat
      781 accctagaaa aaggagaaca aaatggctaa tatcacactt gccgttactg gctctatttc
      841 agcctacaaa gcagctgacc taactagtca attgaccaag ctcggtcatc aggttacagt
      901 cctcatgagt cgctctgcta tggacttcat cacacctttg actctccaat ccttgtcaaa
      961 aaatctggtt cacacagatg tcatgattga agaaaatcct actcttatca agcatatcga
     1021 tattgccaaa gaaacagacg tatttttagt tgcccctgct tctgccaata cgatagctaa
     1081 attagctaat ggtatggctg acaacattat tacggcaaca gctctggctc tgccaattgg
     1141 tactaaaaaa ctgctggcac ctgctatgaa tactaacatg tacctcaatc ctgctaccca
     1201 gaaaaatatc aaaaccctca tagagtatgg ttttgaggaa atcaaaccgc gtgaagccct
     1261 gcttgcttgt ggagattttg gaactggggc cttggctgag gtaactgata ttttagagaa
     1321 agtgagtaac acccttgatg aaaaaaaata aatctactca aattgctaca attgcaatct
     1381 tctttgctgt catgattgtc atcaatatat tgagttcaat tatttttaat atattgcctg
     1441 ttccaataaa acccaccatc gtacatatcc cagtcattat tgcttctatt atatacgggc
     1501 cacgtgttgg ggcttgtttg ggggctctca tgggcttaat gagtatgacc cataatacta
     1561 ttatcttact accaaccagc tatcttttct ccccatttgt tgaaaatggt aatgtttatt
     1621 caatcatcat tgcagttgtc ccacgtatat tagtcgggat tacaccttat ttcgtctaca
     1681 aatggataaa aaatcgtaaa ggattattta ttgctggtgc aattggttca atgaccaata
     1741 ctatttttgt acttggaggt attttcttcc tcttctcctc tgtctttgat ggtaatattc
     1801 aagcaatgtt agcaaccgtt ttaggtacca attcaattgc agaaatgatc atttcttctg
     1861 tattaactgc agcgcttgtt ccaactttac agaaaattca aaaataaata tcagaaaatc
     1921 atgtgaaagc gtgatttttc ttttcaaaca tggtataatg aaagcgttta atagaaaaat
     1981 ttttaaggag atatgcgatg acttatcaag aaacctatca aacatggctc gactttgcgg
     2041 accttccaga ttacttgcgt gaagaattag tcgcaatgga tgaaaaaaca aaagaagatg
     2101 ccttctatac aaatcttgaa tttggtacag ctggtatgcg tggctatatt ggcgctggta
     2161 ccaaccgcat caacgtattc gtagtccgtc aagctactga aggtttggca aaattggtag
     2221 aatcaaaagg tgaagaagct aaaaaacgtg gtgttgccat cgcttacgat tcacgtcact
     2281 tctcgccaga atttgctttt gaatcagctc aagttttggc agcgcatggt atcaaatctt
     2341 atgtatttga aagccttcgt ccaactcctg agttgtcatt cgctgtgcgc cattacaatg
     2401 ccatcgcagg gattatggtg actgcaagcc acaacccgaa agaattcaac ggctacaagg
     2461 tttacggtga agacggcgga caaatgccac ctgctgatgc agatgcattg accaacttta
     2521 tccgtgccat cgacaatcca tttgcagttg aattggctga ccttgaagcc agcaaggaaa
     2581 atggtttgat tacggttctt ggcgaagaaa ctgacgttaa atatcttgaa gaactcaatg
     2641 acctcaacat caaccctgaa ttgattgctg aatacggtaa agacatgaag attgtctata
     2701 caccgcttca tggtacgggt gaaatgttgg cgcgtcgtgc ccttgcacag gctggttttg
     2761 aatctgtcca agttgttgaa gcacaagcaa ctgctgatcc tgacttctct actgtcgctt
     2821 caccaaaccc agagagccaa gcagcctttg cccttgcaga agaactcggc cgtgaagtcg
     2881 gggcagatgt ccttcttgca actgaccctg acgctgaccg tgttggtgtt gaagttcgtc
     2941 aagctgacgg ttcttactgg aacctgtctg gtaaccaaat cggcgctatc attgctaagt
     3001 acatccttga agcccacaag caagcaggta cacttcctgc aaacgctgcc cttgctaagt
     3061 caatcgtatc tactgagttg gtaactaaga ttgctgaaag ctatggcgct accatgttca
     3121 acgtcttgac tggtttcaaa ttcatcgctg agaaaatcca agagttcgaa gaaaaacaca
     3181 accatactta catgtttggt ttcgaagaaa gctttggcta tttgattaaa ccatttgtac
     3241 gtgacaagga tgctatccag gctgtgctta tggttgctga aattgctgcc tactaccgtt
     3301 cacgtggcat gaccttagct gacggtatcg atgaaatctt caaagaatac ggctactttg
     3361 ctgagaaaac aatctctgtt accctttctg gtaaggatgg tgcagaacaa atcaaggcaa
     3421 tcatggctaa gttccgtgat aactcaccag cacaattcaa cgctactgac atcgcggtct
     3481 ttgaagactt tgcccttcaa actaagacag acaaagatgg aaacattgaa aaactcacta
     3541 ctcctccatc agatgtcttg aaatacacct tggcagatga ttcttggttc gctgttcgtc
     3601 cttcgggaac agaaccaaaa atcaaattct acatcgcaac cgttggtgaa acacttgctg
     3661 aagcagaaga aaaaatctcc aacatcgaaa aagaaatcaa cgaatttgtt gggtaagagt
     3721 cacataaatt agaaagtaca aaccacccgt gaaaacgggt agtttgtaca c
//
LOCUS       DASFSW010000160         3588 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.161, whole genome shotgun sequence.
ACCESSION   DASFSW010000160 DASFSW010000000
VERSION     DASFSW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.161"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(202..1557)
                     /locus_tag="U1099_001921"
     CDS             complement(202..1557)
                     /locus_tag="U1099_001921"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEM4868203.1"
                     /translation="MKVIKFGGSSLASAEQLEKVFNIVQSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYKEYVNGKDVTASQEWIINRYQAMVDELGFTANSMKKIADSIISLAS
                     LPIDDNEFLYDAFLAAGEDNNAKLIAEYFTHRGLPARYVHPKKAGIIVSSEPGNARIL
                     PSSYDKIEELRDTDEVLIIPGFFGVTVDNQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIKNPHSIKELTYREMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPTHPGTRIVHKHTEQTVPVVGIAADDGFVSINLSKYLMNREVGFGRKVLQILEDL
                     NIRWEHMPTGIDDLSIVVRERELTPIKEEEILRQLNTKMEVDKAEIEHGLSIIMIVGE
                     NMKSHVGVTATATTALSKQNVNLAMISQGASEVSVMFVVKTEEKKRALHALYEAFFNE
                     Q"
     gene            1734..2375
                     /locus_tag="U1099_001922"
     CDS             1734..2375
                     /locus_tag="U1099_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM4868204.1"
                     /translation="MVKAIIFDMDGVLFDTETFYFQRRADFLATKGLSVDHLDPSIFV
                     GGRASQIWQRILGDDYDNWDVPALEEEYRVYKEKRPTPYAERIFPEVKAVLERLTIQG
                     IPVVLASNTDRSEIERALLDAGIRFHFSHVFSAMDCEAPKPDPTVYMKAASATGVAKS
                     DILVFEDSAKGIEAAKKAGLTVWAIRDQHYGIDQSKADKLVDNLEEAFDLLRF"
     gene            2814..>3588
                     /locus_tag="U1099_001923"
     CDS             2814..>3588
                     /locus_tag="U1099_001923"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEM4868205.1"
                     /translation="MAYQTIRYEVIDSVAILTLNRPEVANGFNIPMCEEILEAIRLAE
                     GDASVKILQIQAQGSIFSVGGDLVEMKRAVDDDDIASLVKIAELVNDISFAMKKLSKV
                     VIMVTDGAVAGAAANMAVAADFVIASSKTKFIQAFVGVGLAPDAGGLFLLSRAIGANR
                     ASHLAMTGEGLSADKALEYGIVYKLAEPEKLEKTVAQVIKKLLRGSVNSYAAIKQLTW
                     ESQFKDWESYRRLELDLQETLAYKEDFKEGVRAHAERRRP"
BASE COUNT         1086 a          637 c          790 g         1075 t
ORIGIN      
        1 aaataaaaaa atagcttaga attgcatcgt cagaaacgtt gattctaagc tatttttgtt
       61 tattcaaatt ttaattgcta ctcaatgaaa attaaaatca gactagctcc aatggagctt
      121 gatactcatc aaatcaagtc aacaacgtct ggttttgatt ttcgaagagt atgaagaggg
      181 aactgaccga cctagttgtg attattgctc gttaaaaaaa gcttcataga gtgcgtgcag
      241 agcgcgtttc ttttcttcgg ttttgacaac gaacataaca gaaacttcac tggctccctg
      301 tgaaatcatt gccaagttga cattttgttt agagagggca gtggtagcgg tagctgttac
      361 accgacatgg ctcttcatat tttcaccaac aatcatgata atagataaac catgttcgat
      421 ttcggccttg tcaacttcca ttttcgtatt gagttgacga aggatctctt cttctttaat
      481 aggagtcaat tcacgctcac ggactacgat ggataaatcg tcaataccgg tcggcatgtg
      541 ctcccagcgg atattgagat cttcgagaat ttgcaacacc ttgcgaccaa aaccaacttc
      601 gcggttcatc aagtatttag acaagttaat gctgacaaaa ccatcgtctg cagcaatacc
      661 gacaacagga actgtttgct cggtatgttt gtgtacaatt cgggtaccag ggtgagttgg
      721 gttattggtg tttttgataa ccaatggaat ccgcccgcga taggcaggaa ggagggcttc
      781 atcgtggagg acggagaaac ctgcataagc taattctcgc atttcacgat aggtcaattc
      841 tttgattgaa tgtggatttt taatgatacc aggatgggca gcaaaaatac catcgacatc
      901 tgtaaagttt tcatagaggt cagccttgac tccggcagca atgatggaac ctgtaatatc
      961 cgatccacct cgtgagaagg tacaaatctg gttatcaaca gttacaccga agaatcctgg
     1021 gataatgagt acttcatctg tatcacgaag ctcttcgatt ttgtcataac ttgaaggcaa
     1081 aatgcgagca tttcctggtt cggagctgac aataatacca gcttttttag gatggacata
     1141 gcgtgcgggc aatcctctgt gggtaaaata ttcggcaatc aatttggcat tattatcttc
     1201 accagctgct aagaatgcgt cgtaaaggaa ttcgttatca tcaattggta ggctggccaa
     1261 agagatgatg ctgtctgcga ttttcttcat gctgttagct gtgaaaccaa gttcgtcaac
     1321 cattgcctga taacggttga tgatccattc ttgactggca gtcacatctt ttccgttaac
     1381 atattcttta taatatttaa tcaatgcatc agtaacttta gtatcctcgg cattgcgttt
     1441 tccaggtgca gacaccacaa caaagcgacg ttctgaatca gattgaacaa tattgaaaac
     1501 tttttctaac tgttcagctg aagcaagtga gcttccccca aatttaataa ctttcattct
     1561 catctccttg gatatttttt tctattatag cagaatgata tgaaattgtc agtatgttca
     1621 gacaaatctt gccaagaatt ttatcgaaaa acgaacaaga gacaagtatg aaaatttcag
     1681 aaaaacattt ttttctttca actgtgttac aattaaagta ataggaggcg agtatggtaa
     1741 aagcaattat tttcgacatg gatggtgtac tttttgatac agaaacattc tattttcaac
     1801 gtcgagcgga ttttttagcg acaaaaggtt tatcggttga tcatttggat ccatctatat
     1861 ttgtcggtgg aagggctagt caaatttggc aacggatttt aggggatgat tatgataact
     1921 gggatgtccc tgccttggaa gaggaatatc gtgtatataa agaaaagcga ccaaccccgt
     1981 atgcagagag gatttttcct gaagttaaag cggtcttgga gagactaact atccaaggta
     2041 ttcctgtagt tttggcttca aataccgacc gttcagaaat cgaacgtgct ctactagatg
     2101 ctggtatacg ctttcacttt agtcatgttt tctcagcgat ggattgtgag gccccaaaac
     2161 cagatcccac agtttatatg aaagcagcta gtgcaacagg agtagctaaa tcggatattt
     2221 tagtatttga agatagtgcg aaggggattg aagcggctaa aaaagcaggt ttgacagttt
     2281 gggctattcg agaccagcat tatggaattg atcagtcgaa agcagacaaa ttggtagaca
     2341 atcttgaaga ggcctttgac ttattgcgat tttaagaaat gttatatttt agtccaagta
     2401 ttagttctcg gtgaaaaaat ggtatttcta gctcagaatc cagttagatt ttccaaaagt
     2461 attaacgtga aaaaatgaaa gttgtaaatt aaacttagag cctatattta cagttcagac
     2521 ttgcatattg aggttgtttt ttcaaaatac agtcagtatt tcccgcaaaa actactttga
     2581 tgagtgaaaa agccaaggcg ctttgcgtgt gaatataggt tcttagacac gttattttct
     2641 agcatatcta aattttatat tgcgatgata gcccccatct ttttcggacg tcacacttta
     2701 cttgctagaa aaaaagtgac aattataaaa actgtgttac aatggaaaac aagaatttga
     2761 agtttgatat tcaaatcttt taattatcaa actattttga aaggagcaag agaatggcct
     2821 accaaacaat tcgttatgaa gtaattgatt ccgtagcgat tctaacttta aacagaccgg
     2881 aagtggcaaa tggttttaat attcctatgt gtgaagaaat cttagaagca atccgccttg
     2941 cagaagggga tgcctccgtc aaaattttac aaattcaagc acagggctcc attttctccg
     3001 tgggtggaga tttagttgag atgaagcgtg cagttgatga tgatgatatt gcttctttgg
     3061 taaaaatagc agagctggtg aatgatattt cgtttgcgat gaagaaattg tctaaagtag
     3121 tcattatggt gacagatggg gcagtggctg gagccgcggc taatatggca gtggcagctg
     3181 attttgttat tgcctccagc aagacaaaat tcatccaagc ctttgtaggt gttggacttg
     3241 cgccggatgc gggggggctc tttctactga gtcgtgctat tggggctaat cgtgctagtc
     3301 acttggcgat gacaggagag ggcttatcag cggataaggc tttagaatat gggattgttt
     3361 ataaattggc agagccagaa aaactagaaa agacggttgc ccaggtcata aaaaaactct
     3421 tgcgaggctc agtaaactct tatgcagcaa tcaaacaact gacatgggaa agtcaattta
     3481 aagactggga gtcttatcgc cgattggagc tggatttgca agaaacattg gcctataaag
     3541 aggattttaa agagggagtt cgagcgcatg cagaacgacg tagaccag
//
LOCUS       DASFSW010000161         3546 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.162, whole genome shotgun sequence.
ACCESSION   DASFSW010000161 DASFSW010000000
VERSION     DASFSW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.162"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..873
                     /locus_tag="U1099_001924"
     CDS             <1..873
                     /locus_tag="U1099_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4868206.1"
                     /translation="IGQAFGGESIDIAPDSTTFLNVLDLSDENMDEDPVKVKSEFLLS
                     WIGKLLDRKMDGREKSLIDRVTRLTYKHFDTPSLVEWVFVLAQQPEQEAKDLALDMEL
                     YVEGSLDIFSHRTNIKTDSHFLIYNVKKLGDELKQIALMVIFDQIWNRVVKNQKLGKK
                     TWIYFDEMQLLLLDKYASDFFFKLWSRVRKYGAIPTGITQNVETLLLDANGRRIIANS
                     EFMILLKQAKSDREELVHMLGLSKELEKYLVNPEKGAGLIKAGSTVVPFKNKIPQHTK
                     LFDIMSTDPEKMRT"
     gene            875..>3546
                     /locus_tag="U1099_001925"
     CDS             875..>3546
                     /locus_tag="U1099_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEM4868207.1"
                     /translation="MKEDKKLVKQARQNFRSNLKSARMHYRKEVRTSKQTVPKKGRFR
                     KSAKNSLFQEKKTELKGNLLSSQKEAEEKFIKEITYVSPRLLKVKEIKNYRLPQAQER
                     LRTARKHLSEVKLSEKQKAVNPKFTFQKENPSPKSRFQFHQEKSFDRLSAEKDVSSAK
                     REVKQLKKVQKTKKNSTKVKVGLGLVASESLDLIAQEDDLDGLRSMKDISLKARRYGR
                     FTFQAGKVVVKSGQTGVRFTKTKFSHGKERFQNFKKGKGFTRQKPLKPRRRYQTFLKH
                     VRKQSVAGIKGIVQAIKGSVTFFSVLAGNPLTWIVSGILLILLLMMSFFMSVSGSSVI
                     QQDEMELTKAYTHMTWEDAEHTRTNEKGIAYYTKIDEIMVYMNHQYQDYKLDDFMETG
                     GVTYKAFLSQVWTDLNGGDSIKSMSDLYKEPTYKLSDEDQEELKELTEGGNYLALQEL
                     DNPFQGQTDEDSLSMTYRYGYEVIDEKPTLHHHIILEAKEGQVIVAPMDGKVSLDGEN
                     VVLTSGKGVNKTKLTLFGIHSGRVSEHQHVLAGDIIGQTKDGTGLKVTYQKVDDDTDK
                     LVYVNPAFYFPKVIQVQTTILPTIGQFGGDEFERAKAIYDYLKSKGATNQAIAAILGN
                     WSVESSINPKRAEGDYLSPPVGATDSSWDDEGWLSLNGPTIYNGRYPNILKRGLGLGQ
                     WTDTADGSRRHTLLLEYAKGKHQKWYDLELQLDFMLHGDSPYYTNWLKDFFKNTGSPA
                     SLAQLFLIYWEGNSGDKLLERQTRASEWYYQIEKGFSQPNGGTAQSDPKALEAVRGDL
                     FENSIPGGGDGMGYAFGQCTWGVAARINQLGLKLKGKNGEKIPIISTMGNGQDWVRTA
                     ASLGGEIGTSPQEGAILSFAGGGHGTPTEYGHVA"
BASE COUNT         1152 a          672 c          826 g          896 t
ORIGIN      
        1 atcggtcaag cctttggtgg ggagagtatt gatattgccc cagattctac caccttctta
       61 aatgtcttag atctatctga tgagaatatg gatgaggatc cggttaaggt caagtccgaa
      121 tttctcttgt cttggattgg aaagctcttg gaccgtaaaa tggatggtcg tgaaaagtcc
      181 ttgattgacc gtgtgacaag actcacttat aagcattttg acacaccgtc cttggtcgaa
      241 tgggtctttg tcttagctca acaacctgaa caggaagcta aggacttggc actggatatg
      301 gaactctatg tggaagggtc gctggacata ttttcacatc ggaccaatat taaaacagac
      361 agtcatttcc tgatttacaa tgtcaaaaag ttaggcgatg aattaaaaca aattgccctc
      421 atggttatct ttgaccaaat ttggaatcgg gtggtcaaaa accaaaagct agggaagaag
      481 acctggattt actttgatga aatgcagcta ctcttattgg acaaatatgc ctctgatttc
      541 ttctttaaac tttggagtcg tgtccgtaag tatggggcga taccgacagg cattactcag
      601 aatgtagaaa ctcttctttt ggatgctaat ggcagacgca tcattgccaa tagcgagttc
      661 atgatacttt taaaacaggc taagagtgat cgtgaagagt tggtgcatat gcttggactt
      721 tccaaggaac tggagaaata cttagtcaat cctgaaaaag gggcaggctt aattaaggcc
      781 ggttccaccg ttgtcccatt caagaataag attccacaac acaccaagct ctttgacatc
      841 atgagtacgg accctgaaaa aatgaggaca tgagatgaaa gaggataaaa agctagtcaa
      901 acaggctagg caaaactttc gaagcaactt aaaatcagcc cgtatgcatt atcggaaaga
      961 agttaggacc tcaaaacaga ctgttcctaa aaaaggaaga tttcgaaaat cagccaaaaa
     1021 ttctctgttt caggaaaaga aaacagagtt aaaaggaaat ctactcagta gccaaaagga
     1081 agcagaagag aagttcataa aggaaattac ctatgtttct cctagactgt tgaaggtaaa
     1141 ggaaatcaaa aactatcgac ttccgcaagc tcaagaacgt ttgcggacgg caagaaaaca
     1201 tctgtcagaa gtaaaactaa gtgagaagca aaaggcagtt aatcccaagt ttactttcca
     1261 aaaagaaaat ccttccccga agtctcgctt tcagtttcac caagaaaaat catttgatcg
     1321 actaagtgca gaaaaagacg taagttctgc caagcgtgag gttaaacaac tcaagaaagt
     1381 ccaaaagact aagaaaaact ctaccaaagt caaagttgga ttaggcttag ttgcatctga
     1441 atcccttgac ctgatagcac aggaggatga tttagatggt ctaagatcca tgaaggatat
     1501 tagtttgaaa gccagacgct atggaaggtt tacctttcaa gcaggtaagg tggtagtaaa
     1561 aagtggacag acaggtgtga ggtttaccaa aacaaaattt tctcatggaa aggaacgatt
     1621 ccagaacttc aaaaagggaa aaggattcac acgccagaaa cctcttaaac caagaagacg
     1681 ttaccagacc tttttaaagc atgtgagaaa acaaagtgtc gcaggtatca aaggaatcgt
     1741 ccaagccatt aagggaagtg tgactttctt ttctgtcctt gcgggaaatc ctttgacttg
     1801 gattgtctca ggcattctct tgatactcct tttgatgatg agctttttca tgagtgtttc
     1861 aggtagtagt gtcattcaac aagatgagat ggagttgacc aaggcctata cccacatgac
     1921 gtgggaagat gcggaacata cgagaaccaa tgaaaaaggg attgcctact acaccaagat
     1981 tgatgagatt atggtttaca tgaatcatca ataccaagac tacaagcttg acgatttcat
     2041 ggaaacaggt ggtgtgacct acaaagcgtt tctcagtcaa gtgtggacag acttaaatgg
     2101 tggagattct attaaatcca tgtctgactt atataaagaa cctacttaca agctgtctga
     2161 tgaggaccag gaagagttaa aggagttgac agaaggaggc aactatctag cccttcaaga
     2221 attggacaat ccctttcagg gacagactga cgaggatagc ttaagcatga cctaccgata
     2281 tgggtatgag gtcattgatg agaaaccaac gctgcatcac catatcatct tagaagcgaa
     2341 agaaggtcaa gtcattgtag ctccgatgga tggcaaggta tctcttgatg gagagaacgt
     2401 tgttttgaca tctggtaagg gagtgaataa gactaaacta accttgtttg gcattcattc
     2461 aggccgagtg agtgaacatc aacatgtctt ggcaggagat attattggtc agaccaaaga
     2521 cggaacgggt ctaaaagtca cctatcaaaa ggttgatgat gatacggata agttggtcta
     2581 tgtcaatcca gctttctact ttccaaaagt aatccaggtt cagaccacca ttcttccaac
     2641 tatcggtcag tttggcggcg atgagttcga gagagccaag gcaatttatg actatcttaa
     2701 aagcaaaggt gcgacaaatc aagctatagc agctatttta ggaaattggt cggtagaatc
     2761 ttccattaat ccaaaacgag ctgaaggcga ctatttatct ccgccagttg gtgcgactga
     2821 tagttcgtgg gatgatgagg gctggctctc acttaatggt ccaactatat acaatggacg
     2881 ttacccaaat attctcaaac gtggtttagg cttaggccaa tggacagata ccgcggatgg
     2941 gtcacgcaga catactttat tgttggaata tgccaaagga aaacatcaaa agtggtatga
     3001 cttagagtta caactggatt tcatgttgca tggggacagt ccttattaca ccaactggtt
     3061 aaaagatttc ttcaaaaata caggtagtcc agctagtctt gcccaactct tcctcattta
     3121 ctgggaagga aattcagggg ataagctact tgagcgtcag acacgggcaa gtgagtggta
     3181 ttaccaaatt gaaaaaggct ttagtcaacc caacggtggg acagcacaaa gtgatccaaa
     3241 agcacttgaa gctgtacgag gagacctttt tgaaaactct attccaggag gtggtgacgg
     3301 gatgggttac gctttcggcc aatgtacttg gggagtcgca gcccgtatca accaactggg
     3361 gctaaaactc aaaggtaaaa acggtgagaa gattccaatt atcagtacca tgggcaatgg
     3421 ccaagattgg gtacgaacag ccgcaagtct cggtggggag atagggacaa gtccacaaga
     3481 aggagctatc ctttcctttg cgggaggagg acatggcaca ccaacagaat acggacatgt
     3541 ggcttt
//
LOCUS       DASFSW010000162         3530 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.163, whole genome shotgun sequence.
ACCESSION   DASFSW010000162 DASFSW010000000
VERSION     DASFSW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.163"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..2065)
                     /locus_tag="U1099_001926"
     CDS             complement(<1..2065)
                     /locus_tag="U1099_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEM4868208.1"
                     /translation="MKKKTTLFALTGIILSCHLFLAGCQKTENTAINHQDVQSSNISK
                     VDENTIVVSEIKSNGYMRLYGEESYFFEGPIPYAAHFLKDTLPDSDYKLNKEDIQYQL
                     EKGYIIKVNEQYFYYPKDKDANVISISEAKKLTKNQKKPTSQAKKGVAGVDYPTDDGF
                     LFENEGQILSKTDEGLVLEHNGHSHFIFYKDLKESKWSYLVPKEYIENSQSSSHSNAK
                     QAKLSQTFDDGYVFDPKDIVAEDANGYTVRHGDHYHYIWKSSLQYGQGNTGETIGKKG
                     QITVLDNHSLHTPTTRNNFVPLKSTTEKGHTSTQSNSKKAFPGIDYPTSDGFIFDGSG
                     VQGQTALGLLIGHGTHTHLLPYSHLIGSPWESYIPTQYLETARAEYHSSTSTQVDVEI
                     PLSPQEGVKPNSSVEEEREAKKSYLAENMHVSKEKIKVIETDAGPAFVYPHGDHSHTI
                     LIEKVEVGKPIEDPHGDPHAHDKIGMATLKQLGFDDEIIEDILHATADTPFPSNETDS
                     EKMREWLKTVKYLNIGQRKDPLKRAGLDLMPNVEVLGIGFTPIDDVKPILQFKKLKQL
                     WMTSTGVKDYQFLKEIPTLEGIDLSQNGVSDLGFLEEFPNLKVVSAAGNDIEDITILA
                     KLKALESLNLDHNKVTDLSPLADLSQLTAVSLDNNRITDLSALQNKKKLTRLYISQNP
                     QLDIST"
     gene            2450..3202
                     /locus_tag="U1099_001927"
     CDS             2450..3202
                     /locus_tag="U1099_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868209.1"
                     /translation="MENTSTQTFEELVAEQKKKRNRVLRIIITLFTTIIVFFACLALF
                     KYNSGNISGTWRNSQAEQDIVETSIKELQNQLPGIDKTWIDATYTMTVVDKKAVFTAT
                     TSFDIPKIAKLTLDQAYKVMLEEAQKEASSLGISVEEYLTTALGADYEKQLKSFLPTQ
                     AEAEKEFHKYQDEYAKELGATYNQETGTISATFFEGEVDQLFHTVTITKINDAAIKQL
                     TTYDISVKDIISYSRKGNTTIINGNEEFTLEK"
BASE COUNT         1137 a          668 c          595 g         1130 t
ORIGIN      
        1 gagttgatat atccaattga ggattctgtg atatatacaa acgggttaac ttcttcttgt
       61 tttgaagcgc agataaatct gtgatacgat tattatctaa actaacagct gtcaattgtg
      121 acaaatctgc taatggggat aaatccgtca ctttattatg atctaaattt aaggattcca
      181 aagcttttaa tttagctaga atagtgatat cttcaatgtc atttcctgca gcagaaacta
      241 cttttaagtt tggaaattct tccaaaaagc ccaaatcaga aacaccattt tgtgataagt
      301 caattccctc taatgttgga atttctttca aaaactgata atccttaacg cctgtgctag
      361 tcatccataa ttgtttcaat tttttaaatt gcaagatagg ttttacatca tcaattggcg
      421 taaaaccaat ccctaacact tcaacatttg gcatcaagtc cagaccggct ctctttagcg
      481 gatcttttcg ctgaccaata tttagatatt ttaccgtttt taaccactct ctcatcttct
      541 cggaatctgt ttcattagat gggaaaggag tatcggccgt agcatgcaaa atatcttcaa
      601 taatctcatc atcaaaacct aattgtttta aagtagccat tccaatttta tcatgagcat
      661 gtgggtcacc gtgagggtct tcgattggtt ttccaacttc aactttctca atcaagattg
      721 tatgagaatg gtcaccatga gggtaaacga aagcaggtcc tgcatctgtt tctatcacct
      781 tgattttttc ttttgaaaca tgcatgtttt cggctaggta gctcttttta gcttctcgtt
      841 cttcttctac acttgagtta ggtttcacac cttcttgtgg tgacaacgga atttctacat
      901 caacttgagt ggaagttgac gaatgatatt ctgctctagc agtctctaaa tactgagtag
      961 gaatataaga ctcccacgga cttcctatca aatgactgta cggaagtaaa tgagtatgcg
     1021 taccatggcc aatgagtaac cctaatgctg tttgaccttg aactccacta ccatcaaaaa
     1081 tgaaaccgtc gcttgttgga taatcaatac ctgggaaagc tttttttgaa ttagactgag
     1141 tggaagtatg ccctttttct gtggtagact tgagtgggac gaaattattt cttgtggtcg
     1201 gagtatgcaa cgaatgatta tcaagaacag taatctgtcc cttttttcca atggtctccc
     1261 cagtatttcc ttgtccgtat tgcaaacttg atttccaaat ataatgataa tgatctccat
     1321 gacgtaccgt atagccattc gcatcttcag ccactatatc cttcggatca aatacataac
     1381 catcatcaaa agtttgtgat agcttcgctt gctttgcgtt ggaatgcgag ctggattggc
     1441 tattctcaat gtactctttg ggtaccagat aggaccattt gctttctttt aaatccttat
     1501 agaaaataaa atgtgaatga ccattatgtt ctaagaccag tccctcatcc gtttttgaaa
     1561 gaatttgacc ttcattttca aaaagaaatc catcatccgt aggataatca acaccagcta
     1621 cccctttttt agcttgagaa gtaggtttct tttgattttt ggtgagtttc ttagcttcag
     1681 aaatagaaat tacattagca tctttatcct tgggatagta gaagtactgc tcatttactt
     1741 ttataatata acctttctct agctgatact gaatatcttc tttattcaat ttataatcag
     1801 aatctggaag ggtatctttt aaaaagtgtg ccgcatacgg gatcggacct tcaaaaaagt
     1861 atgactcttc tccatataga cgcatatagc cattagattt aatttcagac actacaatcg
     1921 tattttcatc tacttttgag atattgctac tttgaacatc ttgatgattt attgcagtat
     1981 tctctgtttt ttggcaaccc gccaaaaaaa gatgacaaga taggataatg cctgtcaagg
     2041 caaataaggt tgttttcttt ttcatttttg tcctcctaaa ctcattaacc agttaacaat
     2101 taactagtat acattttatt tttgattttt gcaagaaaaa acaactgaca atgattgttc
     2161 gtgaataaat tttccattcc acgacaaacg catgaaaaat ttgttttata aaccatattt
     2221 tcacgactgc tgtcagtttt tagtagaaaa aatgtaatat aatagccttg gaaatggggg
     2281 tatccttgtt aaagtgcaag ttttacaaaa acctttaaaa aaatgcatac aacaagaaaa
     2341 tagttacata cctttttctt catgcggatt gcatgtttcc atccctacga tcattgacaa
     2401 agcaaacaaa ctatagtata ttaaaaatag ggaaataaaa ggagaatata tggaaaatac
     2461 atctactcaa actttcgaag aactcgttgc ggaacagaaa aagaaacgta atcgcgtcct
     2521 tagaattatt atcacactct ttaccactat tatcgtattt tttgcctgtc ttgcattatt
     2581 caaatacaac agtggcaaca ttagcggtac gtggcgtaat tcccaagctg aacaagatat
     2641 tgtcgaaacc agtatcaagg aattgcaaaa tcaacttcca ggtatcgata aaacgtggat
     2701 tgatgcaact tatacgatga cagtcgtaga taagaaagct gtctttaccg ctacaacaag
     2761 ctttgatatt cctaaaatcg caaaattaac cttggatcaa gcctataagg tgatgttaga
     2821 ggaagcacaa aaagaagcaa gttctctggg aatttccgta gaagaatact taacaaccgc
     2881 tctcggagca gactatgaaa agcaattgaa atcatttcta cctacacaag ctgaggcgga
     2941 aaaagaattt cataagtacc aagacgaata tgctaaagaa ctaggtgcca catacaatca
     3001 agaaactggt actatctctg ctaccttttt cgagggagaa gtcgatcagc tcttccatac
     3061 cgtgaccatt acaaaaatta atgacgcagc aatcaaacag ctgactacat acgatatttc
     3121 cgtaaaagat attatttcct attctcgcaa gggaaatacc accattatta acggaaatga
     3181 agaatttact ttagaaaaat aataaaaaaa gctgggaaag tcatccctga tggttccgac
     3241 ctcacccaca gaataatgaa aataaatatg aagccctcac tagatgtata atcttagtga
     3301 gggctttgtg cttgacgagt taatgatgtt agtcatcgcc agaaaaacta acccactcta
     3361 ttgattagct ctaaaaaata tgcaatagac atgagtataa gaactagcca ataaaaacac
     3421 agatagattc aagctgattt tgatgaaata cagccgaatt gtctgtgctt ttatttttgt
     3481 ctctactata gataggacaa cattgcggcg atatttgaaa gaacttgtac
//
LOCUS       DASFSW010000163         3523 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.164, whole genome shotgun sequence.
ACCESSION   DASFSW010000163 DASFSW010000000
VERSION     DASFSW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.164"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(26..1459)
                     /locus_tag="U1099_001928"
     CDS             complement(26..1459)
                     /locus_tag="U1099_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEM4868210.1"
                     /translation="MNQNLSHYQFSPESQYLDRKSARKKPSELLKHLIAFANADGGQL
                     VIGIEDEKQENIITGFKDGRAYPIDDFKKIDREMRETPLDLSFEEIPVINHKGEEDFI
                     LVISVELSSNRVIAAPNDEVYLRQGDETVKLSYEQRTQLSYDKGQRFFEDEVVPDASL
                     EDIDDSLVQDFKNRFDISERSVEEILKARRFLINGKLTKAAILLFGKYPSAFFPQARV
                     RFQRFDGTDMGTGSSFNVIKEVTFDDALPILIIKVRDFIRTQLREFQYLDDSGQFKIL
                     PEYPEFAWFEGVVNAVTHRDYSVYGDHIRVLMFDDRLEIHSPGKLPNIVTVDNIKHER
                     FSRNPRIARTLTEFGWVREMNEGVKRIYSEMESAFLHEPKFSEPGNKVVLTLENNIVS
                     RHLRTRDSLEKQFTDFGDLNADEQLLVHYMYNSGEKMTTAKAIELTGRSRSFVVKMLH
                     HLRDLEIITWFGSSKNDRNQYYLLIDK"
     gene            complement(1504..2661)
                     /locus_tag="U1099_001929"
     CDS             complement(1504..2661)
                     /locus_tag="U1099_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM4868211.1"
                     /translation="MAQEFNGDRLRESRYFRHYSISQLAENVGVSKQMISKYEKNLSK
                     PSSEVLQKIIFELKFPLSFYQTKDKFITTDLGTFYRSKLTSSQTEKKSSEMLKKALAL
                     LANFFEDYVDFPKLAEFESHENASPEEVANQIRKNWGLGDSPISSVLRLLETNGFHIA
                     IINSKSEKVDAFGSFVELKMETDSERYYCILIDQDNNNFFRQQFSLAHELGHWALHAR
                     TVYPQELSNIEYRQMEQEANRFASSFLLPASAFSEDLKGHEEDLAYYLKLKSKWNVSI
                     ASMIYRAKDLGLLNSEQYTRLQKRMSARGWRREEPEDASIPVSRPILAKQAYKLLTDA
                     GLFEKQSIVQQFNLKYGYPLPIEIFSELMDIPKETLLQERNNIVHLKSRTL"
     gene            complement(2661..3371)
                     /locus_tag="U1099_001930"
     CDS             complement(2661..3371)
                     /locus_tag="U1099_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868212.1"
                     /translation="MKYSYDIEREVIIKCLEAMSFTTRHLEQDYKVAISETSQNGIYQ
                     ATWARRSNMLKDLFDNSVVWKVLHIKRGIWQFDPILNIETGELIAFFSKNNFRTIRNR
                     YFKKGTSTHYSLSLLLKNERLYPEQEVEQLSLFDADSSEVKRREADLVRMLGEYCEHV
                     TKIRFLIVDYVGQEAVSASLEDYTPQFLSVSSVDISHLLPSSAGIIRTEAIRDEQVND
                     LVDEPQLVTLKPIVEGIL"
BASE COUNT         1095 a          747 c          546 g         1135 t
ORIGIN      
        1 attactaata gtaccctgac tgtaatcatt tatcaattaa aagataatat tgattgcggt
       61 catttttact tgatccaaac caagtgatga tttctaaatc acgaagatga tgcaacattt
      121 taacaacaaa cgagcgactt cttcccgtca gctcaatggc ttttgcggtg gtcatcttct
      181 caccagaatt gtacatatag tgaaccagca attgttcatc agcattgagg tctccaaaat
      241 cagtaaactg tttctccaaa ctatctcgag tccgtaggtg tcggctgaca atattatttt
      301 caagcgtcag gacaacctta ttcccaggtt ctgagaattt aggttcatgg agaaaagcag
      361 attccatctc agaatagata cgctttactc cttcattcat ctcacgtacc caaccgaact
      421 ctgtcaaggt tctggcaatg cgagggtttc gtgaaaaacg ttcatgcttg atattgtcaa
      481 cggtgacgat attgggtaat ttccctgggc tatgaatctc tagtcgatca tcaaacataa
      541 gcacacgaat gtgatcgcca tagacggaat aatcacggtg cgttacagca ttgacaacac
      601 cttcaaacca agcaaattca ggatactcag gtaaaatttt gaattgccca ctgtcatcca
      661 aatactgaaa ttcccgaagc tgggtgcgaa taaaatctct aaccttgata attaagatcg
      721 gtagcgcatc atcaaaggtc acttccttga taacattaaa actacttcca gttcccatat
      781 ctgtaccatc aaatcgctgg aatcggacac gcgcttgagg gaagaatgct gacggatatt
      841 tgccaaagag taaaattgct gccttggtta atttcccatt gattagaaat cgacgtgctt
      901 taaggatttc ttccacagaa cgctctgata tatcaaaacg atttttaaaa tcctgaacca
      961 agctatcatc aatatcttcc agactggcgt ctggcaccac ttcatcttca aagaaacgtt
     1021 gtcccttgtc atagctgagc tgagtgcgtt gctcgtagct caacttgact gtttcatcac
     1081 cctgacgaag ataaacctca tcatttggcg cggcaatcac tcggtttgac gacaattcaa
     1141 ctgagataac taaaatgaaa tcttcttcac ccttatgatt gattacaggg atttcctcaa
     1201 aagataaatc caaaggagtc tcacgcatct cacgatcaat ctttttgaaa tcgtctatgg
     1261 gataagccct gccgtccttg aaaccagtga taatattttc ctgtttctcg tcctcaatac
     1321 caataactag ttgaccgcca tctgcattgg caaaagcaat caagtgtttg agaagttcag
     1381 acggtttctt tctggctgat tttcgatcta ggtattggct ttctggagaa aattgatagt
     1441 gagataagtt ttgattcata tagttctcct taaatatata aaaaatactt aacaattcat
     1501 gatttacaaa gtcctagatt ttaaatgtac aatattattt ctctcttgta acaacgtctc
     1561 ctttggaatg tccataagct ctgaaaatat ctctattgga agaggatagc cgtacttgag
     1621 attaaattgc tggactatgg attgtttttc aaataagccc gcatccgtca gtaatttata
     1681 tgcctgttta gcaaggatcg gtcgtgatac tggaatactt gcatcctctg gctcttctct
     1741 tcgccatcct ctggcactca ttcttttttg tagacgagta tattgttccg agtttaacaa
     1801 acccaaatcc tttgcacgat aaatcatact ggctatagat acattccact tagacttcaa
     1861 tttcaaataa tatgctaaat cttcttcatg tccttttaaa tcttcagaaa aagcagaggc
     1921 tggtaataaa aaactcgaag caaatctatt agcttcttgc tccatttgtc tatattcaat
     1981 atttgaaagc tcctgtggat atactgttct tgcatgcaaa gcccaatgcc ctaactcatg
     2041 agccaagcta aattgttgcc tgaaaaaatt attattatct tggtcaatta aaatacaata
     2101 atatctctca gaatctgttt ccattttcaa ttcaacaaaa cttccaaaag catccacttt
     2161 ctcagacttt gaattaatta tagcaatatg gaatccattc gtttctagta acctaagaac
     2221 actggaaatt gggctatcac ctaatcccca attctttctt atctgatttg caacttcctc
     2281 tggtgaagca ttttcatgac tttcaaattc tgctaatttc gggaaatcaa cataatcttc
     2341 aaaaaaattc gccaagagag ccagtgcttt ttttagcatc tcactagact tcttctcagt
     2401 ttgtgaggat gttaacttac ttctataaaa agtacctaaa tcagtagtta taaatttatc
     2461 ttttgtttga tagaaggaca gcggaaattt cagttcaaaa attatttttt gcaatacctc
     2521 agaactcggc tttgataaat tcttttcgta tttagaaatc atctgtttag aaactccaac
     2581 gttttccgcc aattggctaa tggaataatg tctaaaatat ctcgattctc gaagtctatc
     2641 tccattaaat tcttgagcca ttataatatt ccttctacta ttggttttaa tgtaacgagt
     2701 tgaggctcat ctactaaatc attcacctgt tcatcacgta tggcctcagt tctaattata
     2761 ccagctgaac ttggaagtaa gtgcgaaata tccactgaac taactgataa aaattgtgga
     2821 gtgtagtctt ctaacgaagc actcacagct tcttgaccta cataatcaac aatcaaaaat
     2881 ctaatcttag tcacatgttc acaatactca ccaagcatcc taaccaaatc agcttctctt
     2941 cttttcactt cagagctatc cgcatcaaaa agagaaagct gttctacttc ttgttcaggg
     3001 taaagtctct catttttcaa gagtaaactt aaggaataat gagtagaggt tcctttttta
     3061 aaatatcgat tacgaattgt cctaaaatta tttttggaga agaaggctat tagttcacct
     3121 gtctcaatat tcagtatagg gtcaaattgc caaatccccc tcttaatatg caaaactttc
     3181 caaacaacac tattatcaaa taagtcttta agcatattcg atcttcgtgc ccaagtagcc
     3241 tgataaattc cattttgact agtctctgag atagcaactt tataatcctg ctccagatgc
     3301 ctagttgtaa aactcatcgc ttccaaacac ttgatgatta cttctctttc aatatcataa
     3361 ctatatttca tttattcacc tcaattcgtt tgttatttaa cttcattata acagaccaag
     3421 gagacaaaaa caagaaaaac taccaaaaaa gtcaaccaaa gtgtgtagaa ttaaataatc
     3481 ccatctcctc agcgatgcta atatctttct ccttacacat act
//
LOCUS       DASFSW010000164         3515 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.165, whole genome shotgun sequence.
ACCESSION   DASFSW010000164 DASFSW010000000
VERSION     DASFSW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.165"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1057
                     /locus_tag="U1099_001931"
     CDS             <1..1057
                     /locus_tag="U1099_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868213.1"
                     /translation="QFNAIAKAPQGHGHAAEYANNVIDKIKYPFQKVEQVGRDNVKNG
                     ADRIVGNQKIQTKYYAKASGTINAAFDKANGGEYRYKGMQLEVPKDQYNESIQLMKKK
                     IKAGKVPGHSEPDDAYLIIRKGNVTYNEAKLVAKGGNIVSFSQQIGKILTKKIADQTV
                     KNVAANTVARQAAGVISFGITVGPDVFDLLQGRISSQQLLKNSLVAGTGLLGGVAGGA
                     VAGAFLGPVGSFVGAVAGGTLAAVGSKVVLDNYIEDDRIEMFAILKEEFIDIVMAMAL
                     SEEEFKEIQNNIFDKKLGERLKDMFQKKQSVKNRMFTRESIIEREVERVISKRTVLAE
                     EDILEAVQIAEKEFAVI"
     gene            1101..2189
                     /locus_tag="U1099_001932"
     CDS             1101..2189
                     /locus_tag="U1099_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEM4868214.1"
                     /translation="MISGIIAEYNPFHTGHKYLLEQAEGLKIVVMSGNFMQRGEPAIV
                     DKWTRAQMALEHGADLVVEMPFLVSVQSADHFAKGAISILHKLGVEKLVFGTEEMLDY
                     QKIADIYVDKSEEMENFVKNLPDNFSYPQKTQAMWQEFAGLTFTGDTPNHILALAYAK
                     AVAGTGIQLSPVQRQGAGFHSEEVETSYASATAIRKGADQLDLVRGFLPSVSLFEEAT
                     KVSWRNYFPLLRYQIATHPDLSQVFQVNEELASRIRSAIGSVATVEELVDAVATKRYT
                     KARVRRVLTYILINAVETPLPEAVHVLGFSTRGQAYLKQVKERVDLVTRIGKEPWDSL
                     TQQADAVYQLGADAMAEQTYGRVPVRVE"
     gene            2191..2571
                     /locus_tag="U1099_001933"
     CDS             2191..2571
                     /locus_tag="U1099_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEM4868215.1"
                     /translation="MKKEMSQLEAYKAEAKERWGQTATYAEFEVRYDASKDHLFAQEM
                     QAIFEAFGKMQSLGAVHPDVQAQVANLQAYITENFYTCTKEILQNIGLMYVEDERFSA
                     NIDRAGGLGTAAFVSQAIAIYCQE"
     gene            2631..>3515
                     /locus_tag="U1099_001934"
     CDS             2631..>3515
                     /locus_tag="U1099_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868216.1"
                     /translation="MKEKICKKLKELAKKPVRRIDWSGERWFVRRYKQGLALAWIVYF
                     LLREELVKEILKPVSNYLMNFSCIQWFYVNKLTVLLVFTALLFLAKSIFSILRWCEVI
                     RYFPLGKKKYPEYDFPFQEELVSFLNRKEERGRNIFWLDGSWGSGKTHFIRTFFENQL
                     YKPREIYYISCFGIRTREQAEKALIEEIEQHSTFGSLDHIPVVSSLVKWSYKILGLDL
                     MKKYSLIIFDDLERVAYSEKQKDKPEDFNDLLGFIDHLANHRNQKVLVLMNKEDMGNT
                     YQKIVEEKFKPVVTNIPSQ"
BASE COUNT         1068 a          615 c          899 g          933 t
ORIGIN      
        1 tcaatttaat gctattgcaa aagctcctca aggacatggg catgcagctg aatatgctaa
       61 taatgtaata gacaagataa agtatccttt ccaaaaagta gagcaagtag ggagagataa
      121 tgttaaaaat ggggcagata gaattgttgg aaatcaaaaa attcaaacaa aatactatgc
      181 aaaagctagt ggaacgataa atgcagcatt tgataaggct aatggaggag aataccgtta
      241 taaggggatg cagttagaag ttcctaaaga ccagtataat gaatcaattc aactcatgaa
      301 gaaaaaaata aaagctggaa aagttccggg tcatagtgag cctgatgatg cctatctgat
      361 tattagaaaa gggaatgtta cttataatga ggcaaaactg gttgccaagg gcggaaatat
      421 tgtttcattt tctcaacaaa ttggaaaaat tcttactaaa aaaatcgctg atcaaacagt
      481 taagaatgtc gcagccaata ctgttgctag acaagcagca ggagttattt cattcggaat
      541 tactgtaggt ccggacgtat ttgatctact gcaaggtaga atatctagcc aacaattatt
      601 aaaaaatagc ctagtagcag gaacaggctt actaggtgga gttgcaggtg gtgcggttgc
      661 tggagcattt ttaggtccag ttggatcatt tgtaggtgca gtagctggtg gaacattggc
      721 tgctgttggt tctaaggttg ttttagacaa ctatatagaa gatgacagaa ttgagatgtt
      781 tgcaattcta aaagaagaat ttattgatat tgttatggct atggcgcttt cagaagaaga
      841 gtttaaagaa attcaaaata atatcttcga taagaaatta ggagaacgac ttaaagacat
      901 gttccagaaa aagcaatctg ttaaaaatag gatgtttact agagagtcaa taattgaacg
      961 agaggttgaa agagttatct caaaacgcac ggttttagca gaagaggata tacttgaagc
     1021 agtacagatt gcagaaaaag agtttgctgt aatttaggta ttaggttgta gatggttgaa
     1081 taaaatctag gaggtagggc atgatttcag ggataatagc agaatacaat ccctttcaca
     1141 cggggcataa atacctgctg gaacaggccg aggggttgaa aattgtggtt atgtcaggca
     1201 acttcatgca acggggtgag ccagccattg tggataagtg gacacgggct cagatggctt
     1261 tggaacacgg ggcagatctt gttgtcgaga tgcccttttt ggtgtcggtc cagtcggctg
     1321 accattttgc caaaggagca atcagcattt tacacaagct aggtgtggaa aaactggttt
     1381 tcggtacgga agaaatgctg gattaccaga aaattgcgga tatctatgtg gataagtcgg
     1441 aagagatgga aaattttgtg aaaaacttgc cagataattt ctcttatcca cagaagaccc
     1501 aggctatgtg gcaggagttt gctgggctga ctttcacagg tgacacgccc aaccatattc
     1561 tggctctagc ttacgctaag gcagtggctg gaacaggcat tcagctcagc cctgttcaac
     1621 ggcagggagc tggttttcat tcggaagagg tggaaacgtc ctacgcctcg gcgacagcta
     1681 tccgcaaggg tgctgaccag ctggacttgg ttcgcggctt tttaccgtct gtcagtcttt
     1741 tcgaagaggc gaccaaggta agctggagaa actattttcc gctgctccgc taccagattg
     1801 cgacccaccc agatctcagt caggtctttc aggtcaacga agaactagcc agtcgtatcc
     1861 gctctgccat tgggagcgta gcgaccgtgg aggagttagt ggatgccgtt gcgaccaagc
     1921 gctacaccaa ggcgcgggtg cggcgggtgc tgacctacat cctgatcaat gcggtggaaa
     1981 ctcccttgcc agaagcggtc catgtcctag gcttttcaac tcgaggccag gcctatctca
     2041 agcaggtcaa ggagcgggtg gacctggtga cgcggatcgg caaagagccc tgggacagcc
     2101 tgacccagca ggcggatgcg gtctatcaac tgggcgctga cgcaatggca gagcagacct
     2161 atgggcgcgt gccggtgagg gtggagtgag atgaagaaag agatgagtca actagaagcc
     2221 tacaaggcgg aagcaaagga acgctgggga cagaccgcga cctatgccga gtttgaagta
     2281 cgctatgatg ctagcaagga ccacttgttt gcccaagaga tgcaggctat ttttgaagct
     2341 tttggaaaaa tgcagagctt gggagccgtt catccagatg tgcaggctca ggtagcgaac
     2401 ttgcaggcct atatcacgga aaatttctac acctgcacaa aggaaatctt gcaaaatata
     2461 ggtcttatgt atgttgagga cgagcgattt tcagccaaca ttgaccgagc aggtggacta
     2521 ggaacagcag cctttgtcag tcaagctatt gcgatttatt gccaagaata aacgaaagaa
     2581 gtctggtgag aactaggctt tttttggtat actaaactga ggaggaagtt atgaaagaaa
     2641 aaatttgtaa gaagttaaaa gaactcgcca aaaagcctgt acgacgaata gattggagcg
     2701 gagagagatg gtttgtccga agatacaagc aaggtttagc tttggcgtgg attgtctatt
     2761 ttcttttgcg agaagagttg gtgaaggaaa ttcttaaacc tgttagtaac tatttaatga
     2821 atttttcctg tatacagtgg ttttatgtaa ataagctgac tgttttgtta gtatttaccg
     2881 ctctgctttt ccttgcgaag agtatctttt ccatcttgcg atggtgcgag gttatccgct
     2941 attttccact gggtaagaaa aaataccctg aatatgactt cccttttcaa gaggaactgg
     3001 tttctttctt gaatcgaaag gaagaacgag gtaggaatat cttttggtta gatggttcgt
     3061 gggggagtgg gaaaacccac tttattcgca cattctttga aaatcaactt tacaaaccgc
     3121 gtgagattta ttatatctcc tgttttggca ttcggaccag ggaacaggcg gagaaggcat
     3181 tgattgaaga gattgagcaa cattcgacct ttggaagttt agaccatatt cccgttgtta
     3241 gtagtttagt caaatggtct tataagattt tgggcttaga cttgatgaaa aaatattctc
     3301 ttattatctt tgatgatttg gaacgtgtag cttatagtga aaagcaaaaa gataaacctg
     3361 aagactttaa cgatcttctt ggtttcatag accaccttgc caaccaccgt aatcaaaaag
     3421 tacttgtctt gatgaataag gaggatatgg ggaatactta tcaaaagatt gttgaagaga
     3481 aatttaagcc ggttgtgaca aatattccaa gtcag
//
LOCUS       DASFSW010000165         3509 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.166, whole genome shotgun sequence.
ACCESSION   DASFSW010000165 DASFSW010000000
VERSION     DASFSW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.166"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            145..1023
                     /locus_tag="U1099_001935"
     CDS             145..1023
                     /locus_tag="U1099_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868217.1"
                     /translation="MIEVRALDKVIKGRTILSDISFEIKSGECVALIGPNGAGKTTLM
                     SCLLGDRKATKGQVLLDGLAPQAQSNKEKVAVLPQENAIPTDLKVKELLRFFQAIYKD
                     SLTDVEIDSLLCFSSEQKNQLAGKLSGGQKRLLAFVICLIGKPKLLFLDEPTAGMDTS
                     TRQRFWEIVHELKEKGVTIFYTSHYIEEVEHTAERILVLHQGRLLRDTTPFAMRSEEQ
                     EKEVTLPIAFAAVVESLAEINEISYKQDTVSFKTRAIEQVWASLQEAGCRISDLEVQN
                     KTLLNSLFDKTREEES"
     gene            1020..1751
                     /locus_tag="U1099_001936"
     CDS             1020..1751
                     /locus_tag="U1099_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4868218.1"
                     /translation="MKALVQVEAVKLSRSLGVFLLSIGMPVIFFLIFSSTVQFDDATM
                     QKAFIQSYMLTMTGFSMSGFALFTFPMMLAEDRNNSWLTFIQHSPLPIWQYYLSKLVR
                     VLLCFVSSIAVVFAVGGLVKGVEMTAQEWVISALLLLVTSIVFLAIGLLLVQIQSEQT
                     MSVVANLLYFVLAIMGGSWMPVSLFPDWVQRICKLMPSYHVNQLVTTYAQEGDFLWKS
                     LLIVLGYAIIFLGIALVMNRKTEQQ"
     gene            1760..2854
                     /locus_tag="U1099_001937"
     CDS             1760..2854
                     /locus_tag="U1099_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEM4868219.1"
                     /translation="MIFEKRKVPLMFFVGMIFLYFPLYYAQYTPNPTAVVLATILFAL
                     VYCSLFYTDHKLYSMLAWFYLIGYIAVMSFWGNLQFSLFIFNLSNMLGWYYKENRPTY
                     RTVSYGLLLLAIILWALFGPVPFEMRAFALVIHFFGLALLIFSWIETKRAALQQRLQE
                     QNASINLLLAENERNRIGQDLHDTLGHVFAMLSVKAELVQTLLDHDQIDQAKKEVADL
                     QTLAKDSMQEVRQIVQSLKQHTVAEELQILQQMLDLAGVDLVVLGGEIAEQLSLPVQN
                     KLALVLRELANNLLKHSQASKCRLVFEKDQQELVVYYEDNGVGFAQLTGQELHTIKDR
                     LVTVKGSLEFISLAKPTRLSIRIPFEEVVE"
     gene            2851..3453
                     /locus_tag="U1099_001938"
     CDS             2851..3453
                     /locus_tag="U1099_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4868220.1"
                     /translation="MRILVAEDQAMLRDALCQLLGFQDAVEAVLQAENGSQAIALLEK
                     EPVDVVLLDIEMPEKTGLDVLEWVRGQALPVKVVIMTTFKRPGYFERAVKADVDAYVL
                     KERSIADLMRTIETVLAGRKEYSPELMDILLTQRSPLTNLESQLLKLVAEGLSNKEIA
                     CQLHLSDGTVRNYMTSILSKLGAENRTAAVRIAQEKGWLV"
BASE COUNT          857 a          771 c          902 g          979 t
ORIGIN      
        1 acttcgtttc ctaggctact tttgattttc gttgagtata atatgacatt tgtcacatga
       61 gtccatgaca aaggcttcta gtggggcttc ccttttctag ttatactatg tatgtaagat
      121 gttcaagaag taggaggtag aaaaatgata gaagtacgag cactagacaa ggttatcaaa
      181 ggacggacca ttttgtctga tatttccttt gaaatcaagt caggagaatg tgtggctttg
      241 attggtccaa acggggctgg aaaaacgacg cttatgtctt gtttgttggg tgatagaaag
      301 gcgacgaaag gccaggtttt gttggacggt ctggctccac aggctcagtc gaataaggaa
      361 aaggtggcag tcttgcctca agaaaatgct atcccgacag acctgaaggt caaggaatta
      421 ctccgctttt tccaagctat ttacaaggat agtctgacag atgtggaaat cgacagtctg
      481 ctctgctttt cctcagagca aaaaaatcaa ctggccggca agctatcggg tggtcagaag
      541 cggctcttgg ctttcgtcat ctgtctgatt ggcaagccca agttgctttt cttggacgag
      601 ccgacggcgg gcatggatac ttcgacccgc cagcgttttt gggagattgt ccatgagttg
      661 aaggaaaagg gtgtgactat tttttatacc agccactata tcgaggaagt ggagcatacg
      721 gcggagcgga ttttggtctt gcaccagggg cggctactaa gagataccac gccatttgct
      781 atgcggagtg aggagcaaga aaaagaagtg acattgccga ttgcttttgc ggcggtggtg
      841 gagagcttgg ctgaaatcaa tgaaatcagc tacaaacaag atacggttag ctttaagacc
      901 cgagctattg agcaggtttg ggctagcttg caggaggcag gttgtcggat ttcggatcta
      961 gaggttcaga acaaaacctt gctcaacagc ctatttgaca agacgaggga ggaagaatca
     1021 tgaaagcatt ggtgcaagta gaagcggtca aattatctag aagtttggga gtttttctcc
     1081 tatctatcgg catgcccgtc atctttttcc tgattttctc atcgacggtc cagtttgatg
     1141 atgcgaccat gcaaaaggcc tttatccagt cctacatgct gaccatgaca ggcttttcca
     1201 tgtcgggctt tgcccttttc acctttccca tgatgctggc agaggataga aacaatagtt
     1261 ggttgacctt tatccagcat tcgcctctgc caatctggca atactatctt tccaaactcg
     1321 tgcgggtctt gctctgcttt gtgtcttcta ttgccgttgt ttttgcggtg ggtggtttag
     1381 ttaagggtgt agagatgaca gctcaagaat gggtaatttc ggctctctta cttttagtaa
     1441 cctctatcgt atttctagct attggcttgc ttttggtgca aattcagtcg gaacaaacca
     1501 tgtcggtggt ggccaacttg ctttattttg ttctggccat catgggtggc tcttggatgc
     1561 ctgtttccct ctttcctgac tgggtacagc ggatctgcaa actcatgccg agctaccatg
     1621 tcaaccagct ggtgaccacc tatgctcaag agggagattt tctctggaaa tccttgctaa
     1681 ttgtcctagg atatgccata attttcttag gaattgctct agttatgaat agaaaaactg
     1741 agcaacagtg aggtgaacta tgatttttga aaaaagaaag gtaccgctca tgttcttcgt
     1801 gggcatgatc tttctctatt ttccacttta ttacgcccag tatactccca atccgacggc
     1861 agttgtccta gcgactattc tctttgcctt ggtttattgc tccctctttt ataccgacca
     1921 caagctctat tccatgctgg cttggtttta cctgattggc tatatcgccg tcatgagttt
     1981 ttggggcaat ctgcaatttt ccctcttcat ctttaacctg tccaatatgt tgggctggta
     2041 ttataaagag aatcgaccga cctatcggac ggtgtcctac gggcttttgt tgctggctat
     2101 tatcctatgg gctctctttg gccctgttcc ttttgagatg agagcctttg cacttgttat
     2161 ccactttttc ggcttggcct tgctgatttt cagctggatt gagaccaaac gggcagccct
     2221 tcagcaacgc ctacaggagc agaatgcttc aatcaatctc cttctggctg agaatgaacg
     2281 caaccgtatc ggtcaggact tgcacgatac actgggccat gtctttgcca tgctgtctgt
     2341 caaggccgag ctggtccaga cacttttaga ccatgatcag attgaccagg ccaagaagga
     2401 ggtggcagac cttcagacct tggccaagga ctccatgcag gaggtccgcc agattgtcca
     2461 gtccctcaag cagcacaccg tggcagagga actccagatt ttacagcaga tgttggacct
     2521 ggcaggagtg gacttagttg tcttgggcgg ggaaatagca gagcagctca gcttgcccgt
     2581 ccagaacaag ctggctctgg tgctgagaga gttggccaat aacctgctca agcacagcca
     2641 ggctagcaag tgccgtttgg tttttgaaaa agaccagcaa gaattggttg tatactacga
     2701 agacaacggt gtgggctttg cccagttgac tggtcaggaa ttacatacta taaaggacag
     2761 gttggtaacg gtgaaaggaa gtttggaatt tatctctcta gccaagccca cccgcctgtc
     2821 catccgaatt ccgtttgagg aggtggtaga atgagaatct tagtcgcaga agatcaagca
     2881 atgctgcggg acgccctctg tcaactcttg ggctttcaag atgcggttga ggctgtttta
     2941 caggcagaaa acggcagtca ggccattgct cttttggaaa aagagccagt agatgtggtg
     3001 ctactagata tagaaatgcc tgaaaagacg ggcctggatg tcttggaatg ggtgcggggg
     3061 caagccttgc ctgtcaaggt cgtcatcatg accaccttca agcgaccggg ctatttcgag
     3121 cgggcggtca aggcagatgt ggatgcctat gtgctcaagg aacgctccat agcagacctc
     3181 atgcggacca ttgaaacggt cttggcgggg cgaaaggaat attcaccaga gttgatggac
     3241 attctcctaa cccaacgcag tcccttaacc aacttggaaa gtcagctgct caagctggtg
     3301 gcagaggggc tgtccaataa ggaaatcgcc tgccagctcc acctgtctga cgggacagtc
     3361 cgcaactata tgacctcaat cctgtccaaa ttaggagctg aaaatcgtac ggctgctgtc
     3421 agaattgcgc aggaaaaggg ttggttagta taaacgaatg agattgggca tttgtccaat
     3481 ctttttatag acctgatttt tgactgagt
//
LOCUS       DASFSW010000166         3483 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.167, whole genome shotgun sequence.
ACCESSION   DASFSW010000166 DASFSW010000000
VERSION     DASFSW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.167"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(90..722)
                     /locus_tag="U1099_001939"
     CDS             complement(90..722)
                     /locus_tag="U1099_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEM4868221.1"
                     /translation="MKEFKTIREDGIVEEEIKKSRFICFMKRVTSEEEARDFINKIKK
                     EHYKATHNCSAFVLGENMEIKRSSDDGEPSGTAGVPMLTVLENHELTNVATVVTRYFG
                     GIKLGAGGLIRAYAGVVANAVKEIGVVEVKEQEGISISMSYTQYQEFANWRAEQGLEE
                     FDAQFTTEVSTMIFVDKEFLEQTLASLTDFYHGKVSAEKANSRIVEVPVY"
     gene            779..2071
                     /locus_tag="U1099_001940"
     CDS             779..2071
                     /locus_tag="U1099_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM4868222.1"
                     /translation="MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTF
                     KEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDFPITKCLKWKGQLTDWQQRISDG
                     LVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
                     DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQA
                     ADNAVKEAGVQVFLTATSTDELDKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDD
                     TLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKKTFPEETIGFVSSQT
                     ENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVG
                     RSIERPTGLLQFYHEGSTGAIEKAIAEIKQMNKEAGYV"
     gene            2064..2729
                     /locus_tag="U1099_001941"
     CDS             2064..2729
                     /locus_tag="U1099_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEM4868223.1"
                     /translation="MSNCLLCGQAMKNKTRFSDLIFFSKEKSGICEECFSTFEEIAEQ
                     HCPHCYKNGESESCKDCQYWQNQGKPVVHTALYQYNQAMAHYFSRYKFQGDYVLRNIF
                     AKKLRIALSQFPDYTIVPIPISQKRLSERGFNQVEGLLDATNIPYQSLLGKYESQKQS
                     SKNRAERLEAKQMFYLLDEKEVPEKILLFDDIYTTGATIQLAVELFMKIGRKEIKTFS
                     LTR"
     gene            2806..3348
                     /gene="raiA"
                     /locus_tag="U1099_001942"
     CDS             2806..3348
                     /gene="raiA"
                     /locus_tag="U1099_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEM4868224.1"
                     /translation="MIKFSIRGENLEVTEALRTYVEEKVAKIEKYFNEEQELNAKVNL
                     KVYRDKRAKVEVTIPVGAVTLRAEDISQEMYGSIDLVVDKIERQIRRNKTKIERKHRQ
                     KVATGQVFTDELVEQTGEEVKVVRTKQVDLKPMDMEEAVLQLELLGHDFFIYTDANDG
                     TTNVLYRREDGDLGLLEVRQ"
BASE COUNT         1190 a          639 c          695 g          959 t
ORIGIN      
        1 gtccagaggt cggggaatag tcggggagtt gtaactataa acagctatat aataagtaaa
       61 aaaaaaaact atctcgccga tagctttttt taatagacag gtacctcaac gattcgagag
      121 ttagcttttt ccgcggatac ttttccgtgg taaaagtcag tgagacttgc taaggtttgt
      181 tcaaggaatt ctttatctac aaaaatcatg gttgaaactt cagtagtaaa ctgggcatca
      241 aattcttcca gaccttgctc agctcgccaa ttggcaaact cttggtattg ggtataggac
      301 atgctaatgg aaatcccttc ctgctcctta acctccacaa ccccgatttc cttgactgca
      361 ttagccacga ctccagcgta agcacgaata agtccgcctg ccccgagttt gatacctcca
      421 aaatagcggg taaccaccgt tgcgacattg gtcagttcgt gattttctaa aaccgtcagc
      481 attggcacac cagcagtacc agacggctcc ccatcatctg aggaacgctt gatttccata
      541 ttttctccaa ggacaaaagc agagcagtta tgagtagcct tgtaatgttc tttcttgatt
      601 ttgttgataa agtcgcgagc ttcctcctcg ctggtcactc gtttcataaa gcagataaat
      661 cgtgactttt taatttcttc ctcaacaata ccatcttctc taattgtttt aaattctttc
      721 ataaccaaat tgtactaatt tttttaaaaa ctgacaagaa attccgaata aataagcgat
      781 gaaagaatta gaaaattatt atggaagatt atttaccaaa taccaattga cagcaaaaga
      841 aagagaaata gcagaaaaag tgccaagtat tacaaaaaag aataactgct ttcgctgtgg
      901 aacaactttt aaagaagaaa acaaattgcc aaacgatgct tattactgtc gagcctgctt
      961 gcttctaggc agagtacggt cagacgaaaa actctatcat tttcctcaga aagattttcc
     1021 aatcactaag tgtttaaagt ggaaaggtca actaactgat tggcaacaaa gaatttcaga
     1081 tggactagtt gcaaacgtgg aaaataatcg tgcgacattg gttcatgcag taacaggagc
     1141 aggtaagaca gaaatgatct accacaccgt tgcctcagtg attgataaag gcggagcggt
     1201 ttgcctagcc agtcctcgaa ttgatgtttg tatcgaactc tataaacgtc tgcaaaatga
     1261 cttttcagtt ccaattagtt tactacatgg agagtctgaa ccctatttcc gaaccccatt
     1321 agttgtagca accacacatc agttattaaa attttatcag gcctttgatt tggttttgat
     1381 tgatgaagta gacgcctttc cctatgcaga taatcccatg ctctatcaag cagcagacaa
     1441 tgcggtcaag gaagccggtg ttcaagtttt tctgacagcg acttcaacag atgaattgga
     1501 taaaaaagtc agaacaggta aattaagtcg tcttagtttg ccaaggcgct ttcatggcaa
     1561 cccacttgtt gtcccgcaaa aagtctggtt tagtaaattc gatgataccc taaagaaaaa
     1621 tagactagtc ccaaagttga aaaaagcgat tgaagaacag agaaagtcgg gctttccctt
     1681 actcattttt gtcccagaaa tctccaaagg tcaagaattt accaagataa tgaaaaaaac
     1741 attcccagaa gaaacaattg gctttgtatc cagtcaaaca gaaaatcgcc ttgaaatagt
     1801 tgaagggttt cgcaagagag aaatcacagt cttaatctcg actactattc ttgaacgtgg
     1861 ggtgaccttc ccatgtgtag acgtctttgt tgttcaagct aatcattacc tctacacagc
     1921 gtcaagtctt gttcagattg caggccgggt cggaaggagt atagaacgtc cgactggttt
     1981 acttcagttt tatcatgagg gaagtacagg agccattgaa aaggcaatcg ctgaaattaa
     2041 acagatgaac aaggaggctg gttatgtcta attgtctatt gtgcggtcaa gctatgaaaa
     2101 ataaaacaag attttcagat ctcatcttct ttagtaaaga aaaatcgggc atctgtgaag
     2161 aatgtttttc gacttttgag gaaatagcag agcaacactg tccacattgt tataaaaatg
     2221 gagaatcgga aagttgcaag gattgtcagt actggcagaa tcaagggaaa ccagttgtcc
     2281 acacagcttt gtaccaatat aatcaagcga tggcacatta ttttagtcgc tacaaatttc
     2341 aaggagacta tgttttaaga aatatttttg ctaaaaagct tagaatagcg ttaagccaat
     2401 ttcctgacta cacaattgtt cccataccaa ttagtcaaaa acgactatca gaacgcggtt
     2461 ttaatcaagt ggaaggactt ttggatgcta caaatattcc ttatcagtcc cttcttggaa
     2521 agtatgaaag ccaaaaacaa tcatctaaaa atcgtgcaga gagactagaa gcaaaacaga
     2581 tgttctatct attagatgaa aaagaagtgc cagaaaaaat attattattc gacgatatat
     2641 atacaacagg agccacaatc caacttgctg tagaactttt catgaaaatt ggtaggaaag
     2701 aaatcaaaac attttcacta acacgataag aaaacggttg caatattcct ataatgtggt
     2761 ataataatag tgaagaaagg cgaaagccaa gaaagaggta ctaatatgat taaattcagt
     2821 attcgtggag aaaaccttga agttacagaa gcacttcgca cctatgtaga agagaaagtg
     2881 gctaagattg agaaatattt caatgaagaa caagagttga atgctaaagt aaatctaaaa
     2941 gtttaccgtg acaagcgcgc aaaagtagaa gtgacaattc cagttggagc agttacactt
     3001 cgcgcagaag atatttcaca agaaatgtat ggctctatcg atcttgtagt agataagatt
     3061 gaacgccaaa ttcgtcgtaa caaaaccaag attgaacgaa aacatcgtca aaaagtggca
     3121 actggtcaag tctttacaga tgaacttgtt gagcaaacag gtgaggaagt aaaagtggtt
     3181 cgtactaagc aagtagactt gaaaccaatg gatatggaag aagcagtcct ccaattggag
     3241 ttgctcggac acgatttctt tatctataca gatgctaatg acggtacaac aaatgtattg
     3301 tatagacgcg aagatggaga tttgggtctt ctagaagtac gtcaataaag ataataaaac
     3361 agttgcagtt tttgcagctg ttttattatt ttcaaaaaac cggttgacaa ttgccaaagt
     3421 agatgataga ataattgagt tgtctcttga agggctagtt aatagaggaa caaaaaaagt
     3481 ttc
//
LOCUS       DASFSW010000167         3461 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.168, whole genome shotgun sequence.
ACCESSION   DASFSW010000167 DASFSW010000000
VERSION     DASFSW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.168"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..508
                     /locus_tag="U1099_001943"
     CDS             <1..508
                     /locus_tag="U1099_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecF"
                     /protein_id="HEM4868225.1"
                     /translation="LDELKRVLKEHVANERLFLEFNINSKETSQHLDILYKEVSVLSL
                     GQKVVAMLDFLLAYSDYSKDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILA
                     THNATIVTNSMTDQVVIMESDGAHAWIESQGYVSEKFIKNHIINQLEGGRDSFKHKMS
                     IYETALSE"
     gene            846..1208
                     /locus_tag="U1099_001944"
     CDS             846..1208
                     /locus_tag="U1099_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868226.1"
                     /translation="MTESYRTIRKEINLTLEEFEQIQLLMRKENAEQFSPFVRQKLLD
                     IVNNKDDIRDWFILWQSQKIEQISRDILQVATLAERGHQVTSEHLRIILTCVQELMTE
                     VEKAVPLSSEFRDKYMGG"
     gene            1211..1576
                     /gene="mobC"
                     /locus_tag="U1099_001945"
     CDS             1211..1576
                     /gene="mobC"
                     /locus_tag="U1099_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEM4868227.1"
                     /translation="MENRYRTNLKKVFLTDQELANLNNHISQSSCQNFSAYARKVLLN
                     PNMTFLTIDTSNYQDLVFELRRIGNNINQIAKTVNQTKLLTVEQRDNLKTGIGQLIAE
                     VDKDFYVKCQKLREFYGSH"
     gene            1563..3446
                     /locus_tag="U1099_001946"
     CDS             1563..3446
                     /locus_tag="U1099_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM4868228.1"
                     /translation="MVVTKHFAVHGNKYRKSLIKYILNPAKTDQLKLVSDFGMSNYLD
                     FPNYEEIVEMYQANFVNNDRLYDSRNDRQQIKQNKIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIKELTGGNFRFIVTTHTDRLHVHNHILINSVDLNSHKKLKWDYAQERNLRMIS
                     DRLSKEAGAKIITPNRYSHEKFVTYRKSNHKFELKQRLYFLMENSKDFDDFLSKAEAL
                     NVQIDFSRKYARFLMTDMPMKRVIRGKQLDKRQPYTEEYFREHFAKRAIEQCLDFLLP
                     RVRDLSQLLEFARELNLAMSLKQKNVAFTLTKNSQSITVNNQKVSSKNLYDVQFFETY
                     FEKRGEVPDIDQSQLISDFEGYCEEQDKEKLAYEDLQEAYQAFKEKRDQVQEFDVVLA
                     DHQIDKLVKDGLFIRMNYGVKKDGLVFIPNRHLDIKETESGKHYHAFIRETAQFFIYN
                     KEASELNRYMRGRELIRQLTNDSQTISKRKRPTIDTLKKKIEEINLLIELGTENKSYQ
                     DIKDEIIKDIAQLDLTITEIQEHIDHLNKVAEVLLNLDNNDIENRRLAKYDYAKMNLT
                     AAIKLEQVEKEIEIYQIKLDKSIDDYEYLIRRLEKFVKILNEIDSSKDELKFEHSEKN
                     VKE"
BASE COUNT         1212 a          577 c          688 g          984 t
ORIGIN      
        1 actagatgag ttaaagagag tgctaaaaga acacgtagcc aatgaaagac tttttttgga
       61 gtttaacatt aactcaaaag aaacatcaca acatcttgac attctttata aagaagttag
      121 tgtattaagt ttgggtcaaa aagttgtagc gatgttagat tttttactag catatagtga
      181 ttattcaaag gatttcagac cattaattat tgatcaacct gaagataatt tggataaccg
      241 ctatatatac agacatttag ttcaacagtt tagagacgtg aaagctcaac gacaaattat
      301 tttagcaacc cataatgcga caattgtaac aaattccatg accgatcaag tggtaattat
      361 ggaatcagat ggggctcatg cttggattga atcacaggga tatgttagtg aaaaatttat
      421 aaaaaaccat attatcaatc aattggaagg tggaagagac tcatttaaac ataagatgtc
      481 catatatgag acagctcttt ctgaataata taaggaaatg aaaagtaggt tagaaatatc
      541 ctactttttg ttttgtgata ccttttccaa atcacctcta ttatcctaat atcattccta
      601 aaaaaatcaa tccatgattt ttcactgggg gtttgggggc gaagccacca agttatctta
      661 tcatcggcta tcaaaactca aaggttttgg tcggctggcg ataagatttt tgggatattg
      721 tggacacaat atctgagctc gcaaaagccg aaaagggaag atttgaggct tttagggagc
      781 ccccgtatca gatatggtat cggtgctaac ccgctagagg gaaatgtgag aaggagtgta
      841 tcatcatgac agaaagttat cggacaattc gtaaggaaat caacttgacg cttgaggaat
      901 ttgagcagat tcaactactg atgagaaagg agaatgcgga gcagttttct ccctttgtta
      961 gacaaaaact attggacatt gtcaataaca aggatgacat cagagattgg tttattctgt
     1021 ggcaatctca aaaaatagaa caaatcagta gagatatttt acaggtagca actctagcgg
     1081 aacgaggcca tcaggtcacg tcagaacact taagaattat attgacttgt gttcaagaac
     1141 tgatgacaga agtagagaag gcagtaccac tcagttcaga atttcgagat aagtatatgg
     1201 gaggttagat atggaaaatc gttatcgaac caatctcaaa aaagtctttt taacagacca
     1261 ggaacttgcg aacctgaaca atcatattag ccaaagttct tgtcaaaatt tttctgctta
     1321 tgccagaaag gttttgctta atccaaatat gaccttttta acaattgata ccagtaacta
     1381 ccaagacttg gtttttgaat tacgtcgaat tggcaataac attaaccaga tagctaaaac
     1441 agtcaatcag acaaaacttc tgacagtaga gcagcgagat aatttgaaaa cgggcatagg
     1501 tcagctgatt gcggaagttg ataaggactt ctatgtcaag tgtcaaaagt tgagggaatt
     1561 ttatggtagt cactaaacat tttgcggttc atggcaacaa ataccgtaaa agtcttatca
     1621 agtatattct aaatccagct aagactgacc aactcaaatt ggtttcagat tttggaatga
     1681 gcaattacct cgacttccct aattatgaag aaatagttga gatgtatcag gcaaactttg
     1741 tcaataatga taggctttat gattcgagaa atgaccggca acaaatcaag caaaataaaa
     1801 ttcatgctca tcatcttatc cagtcattct cacctgagga taatcttaca cctgaagaaa
     1861 tcaatcgtat tggttatgag acgataaagg agttgactgg cggaaacttt cgctttattg
     1921 tgacaaccca tacggatagg ttgcatgttc ataaccatat cctcatcaat tctgttgacc
     1981 tcaattctca taagaaactc aaatgggatt atgctcagga gagaaattta cggatgattt
     2041 ctgaccgtct ttccaaagaa gcaggtgcca aaattatcac accgaaccgt tattctcatg
     2101 agaagtttgt gacctatcgc aaaagtaatc acaaatttga attgaagcaa cgtctttatt
     2161 ttctgatgga gaatagtaag gattttgacg actttttatc caaggcagaa gcattgaatg
     2221 ttcagattga tttttcaaga aaatacgctc gttttttgat gacggatatg cccatgaagc
     2281 gagttattcg aggcaagcaa ctagataaac gtcaacccta taccgaggaa tactttcgag
     2341 agcactttgc caagcgagcg attgagcaat gtttagattt tctcttaccc agagttcgtg
     2401 acctttcaca acttttggag tttgcaagag aactcaacct tgctatgtct ctaaaacaaa
     2461 agaatgtggc gtttactcta acaaaaaata gtcagagcat tacagtcaat aatcaaaaag
     2521 tcagttccaa aaatctctac gatgttcaat ttttcgaaac ttactttgag aaacgtggag
     2581 aagttcctga tattgaccag tcgcaactta tttccgactt tgagggatat tgtgaggaac
     2641 aagataagga aaagctagct tatgaagact tgcaagaggc ttatcaggca tttaaggaga
     2701 aaagagacca ggttcaagag tttgatgtcg tcttagctga ccatcagatt gataagttag
     2761 tcaaagatgg tctctttatc agaatgaact acggcgttaa aaaagatggc ttggtcttta
     2821 tcccaaaccg tcatttagat attaaagaga cagagagtgg taaacactac catgccttta
     2881 tccgtgagac cgcacaattt ttcatttaca ataaggaggc gtccgaactc aatcgctata
     2941 tgcgagggcg tgaactcatt cgtcagttaa ccaatgatag ccagactatc tctaaaagaa
     3001 aaagaccgac cattgacaca cttaagaaaa agattgaaga gattaatctt cttattgagt
     3061 tgggtacgga gaataagtct tatcaagata ttaaagacga gattattaag gatattgctc
     3121 aattagattt gaccattaca gagattcagg aacacataga tcatctcaac aaggttgcgg
     3181 aagtcttgct taacctggac aataacgata tagagaaccg ccgcttggcc aaatatgact
     3241 atgccaagat gaatctaact gcggctataa aactggaaca agttgagaaa gaaatcgaaa
     3301 tctatcaaat taagttagat aagtcaatag atgattatga gtatttaata aggaggttgg
     3361 aaaagtttgt caaaatttta aatgaaatag actcaagcaa ggatgaacta aaatttgaac
     3421 acagtgaaaa aaatgtgaaa gaatagcatt tttcggattt a
//
LOCUS       DASFSW010000168         3456 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.169, whole genome shotgun sequence.
ACCESSION   DASFSW010000168 DASFSW010000000
VERSION     DASFSW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.169"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            62..760
                     /locus_tag="U1099_001947"
     CDS             62..760
                     /locus_tag="U1099_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4868229.1"
                     /translation="MFSGSRLKELRMEKQYSQSELANLLKINRASYNKWESGKSVPNQ
                     KNLSALARILDVPTTYFESEYKIVNTYLQLSTENQGKVDEYAEGLLQAQESHDNVIPL
                     FAVEVLSDVSLSAGFGESLFDEYETETVYAEEEQNGYDIAAWIKGDSMEPIYLDGEVA
                     LIRASGFDYDGAVYALSWNDSVYIKKLYREENGFRMVSLNDNYPDKWIPYEDNPRIIG
                     LVVSHFMPVIGAQA"
     gene            896..2311
                     /locus_tag="U1099_001948"
     CDS             896..2311
                     /locus_tag="U1099_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM4868230.1"
                     /translation="MGYIDYSREPQSDIAFVDMKSFYASVECVDRGLNPLHTSLCVMS
                     RADNAGGLILASSPMFKKVFGKNNVGRAYDLPFDISTRKFDYYNAKRQGLDISPPYVS
                     YIEHWAKRTLIVPPRMDRYIEKNIDIQHIFQDYAAPEDILPYSIDEGFIDLTSSLNYF
                     VSDKQMDRKAKLDVVSAKLQHDIWQKTGIYSTIGMSNANPLLAKLALDNEAKRTPTMR
                     ANWSYEDVETKVWSIPKLTDFWGIGSRTEKRLHKLGITSIKELANSNPDRLKKEFGKV
                     GVQLWFHANGVDESNVHQPYKPKSHGLGNSQVLPRDYVKQRDIEIVLSEMAEQVAIRM
                     RREHQKATIVSIFVTYSKTEMKQPINAQMKIEPTNHTRLLTDTVLTLFRKKYTSGAVR
                     GIAVNYSGFVDEGYALISLFDDIEATEKEENLQRTIDHIRDQFGFLAVQKGTALLDSS
                     RNIARSKLIGGHSAGGLEGLQ"
     gene            2308..2676
                     /locus_tag="U1099_001949"
     CDS             2308..2676
                     /locus_tag="U1099_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868231.1"
                     /translation="MIDRSYLPFQSARDYQDVNMQKWMGFFLSEHTSALSDDDKQVSY
                     LYDLSLNQKLMLLSQSYANQLTIQVTRKQKNGYQTIIGTIPTLSTNEVILKTNDGHIA
                     ITLEDIMAITLVEEVYHESA"
     gene            2663..2962
                     /locus_tag="U1099_001950"
     CDS             2663..2962
                     /locus_tag="U1099_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868232.1"
                     /translation="MNQLDFYKNELQMDYFSENYRKFESDFYRYSALDTPLTFLTDDI
                     MLAMAKSAKNYFKLNKENAKDNRDHYFIFDIENLNDSKLTKKYVYQRTTSIRQKD"
     gene            complement(2989..3276)
                     /locus_tag="U1099_001951"
     CDS             complement(2989..3276)
                     /locus_tag="U1099_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868233.1"
                     /translation="MRLFKKQTHNQDSFKRLIHRLSGMSEADLHKVNQLLDVVFDATR
                     QTIREEKKFPETMPKEPILDETITEAKNKLKSEQLEKRIEQFRQAKKPKNE"
     gene            complement(3321..>3456)
                     /locus_tag="U1099_001952"
     CDS             complement(3321..>3456)
                     /locus_tag="U1099_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEM4868234.1"
                     /translation="DVMAKNNEDILQSQKKIEELHQLIDDKNKQNKKLQAISVAIDDL
                     "
BASE COUNT         1095 a          667 c          643 g         1051 t
ORIGIN      
        1 ttgcatcttg aaaatatttt gatataattg ttgcataata aagcaacagg ggttttgatt
       61 tatgttttct ggtagccgtc ttaaagaact tagaatggaa aagcagtatt ctcaatcaga
      121 gttagctaat ctgttaaaaa tcaatagagc ttcatacaat aaatgggaat ctggtaaatc
      181 tgttcctaat caaaaaaatc tttctgccct tgcaagaata cttgatgttc caacaaccta
      241 ttttgaatcg gaatacaaga ttgtgaatac ctatctccaa ctctctacag aaaatcaagg
      301 aaaggttgat gagtatgcag aaggattgct tcaagcgcaa gaatcacatg ataatgttat
      361 tccattgttt gctgtggaag tattatctga tgtttcactt tctgctggtt ttggggaatc
      421 gctctttgac gaatacgaga cagagaccgt ctatgctgag gaagaacaga atggttatga
      481 tattgccgcc tggattaaag gagattctat ggaacccatc tatcttgatg gtgaagtagc
      541 ccttattcgt gctagtggtt ttgattacga tggtgcagtc tacgccctat cttggaatga
      601 ttctgtctat attaaaaaac tttatcgaga agaaaatggc tttagaatgg tgtcactcaa
      661 cgataactac cctgataaat ggattcctta tgaagacaat cctcgtatca tagggcttgt
      721 tgtgagtcac tttatgccag ttataggagc ccaagcatga cagttaatgt taaagaaatg
      781 atttatctca gggacaatcg tatctatttt acaccctatc tcaaagagta tgatattact
      841 gatcatattc aagaactcat ggaacaatta gaagatctaa aaagaggtta agatgatggg
      901 ctatatcgat tactcaagag aacctcagtc tgatatagct ttcgttgaca tgaaatcgtt
      961 ctatgcttca gttgaatgtg tggatagagg attgaatccc ctccatacct ctctttgtgt
     1021 catgagtcgt gctgataacg ctggagggtt aattttagcc tcttctccta tgttcaaaaa
     1081 agtttttgga aaaaataatg ttggacgtgc ctacgattta ccttttgaca tcagcacaag
     1141 aaaatttgac tactataatg ctaaacgtca aggcttagac atatcaccac cctatgtttc
     1201 ctatattgaa cattgggcta agcgaacgct tattgttcca ccacgtatgg atcgctatat
     1261 cgaaaagaat attgatattc agcacatttt ccaagattat gcggctcctg aagatattct
     1321 cccctactcc attgatgaag ggtttattga tttaacctcc tcgctcaatt actttgtaag
     1381 tgataaacag atggatagaa aggcaaaatt agacgtggtc tctgccaaac tacaacatga
     1441 catctggcaa aagactggta tctattctac cattggtatg agtaacgcta acccattatt
     1501 ggccaaactt gctctagaca atgaagccaa acgaacacct actatgcgtg ccaattggtc
     1561 ctacgaagat gtagaaacaa aagtctggtc aatccctaaa ctaactgatt tttggggaat
     1621 tggttctaga accgagaaac gccttcacaa actagggatt acctctatca aggaactagc
     1681 caatagcaat cctgatcgat tgaaaaaaga atttggaaag gttggagtcc agttgtggtt
     1741 tcatgctaac ggtgttgacg agagtaatgt tcatcaaccc tataaaccaa agtcacacgg
     1801 gcttggcaac tctcaggtgc tacctcgtga ctatgtcaag cagcgtgata ttgagattgt
     1861 tcttagtgaa atggctgaac aagttgccat ccgaatgagg cgagaacatc agaaagctac
     1921 tattgtttcc atctttgtga cctactctaa aacagaaatg aaacagccaa tcaacgctca
     1981 aatgaagata gaacccacta atcatacacg actgcttacc gataccgtat taacgctttt
     2041 tcgtaaaaaa tacacgtcag gagccgttcg gggtattgca gtcaattact caggatttgt
     2101 cgatgaaggc tatgccttaa tctcattatt tgatgatatt gaagcaaccg aaaaagaaga
     2161 aaacttacag cgaactattg accatattag agaccaattc ggttttttag cggttcaaaa
     2221 aggaactgcc ctcttagaca gttccaggaa tattgcaaga agtaaactaa taggaggcca
     2281 ctcagcaggt ggcttggagg gattacaatg attgaccgat catacttacc atttcaatcc
     2341 gcaagagact atcaagacgt taacatgcaa aagtggatgg ggttcttttt atcggaacat
     2401 acctctgcct tatcagatga tgataagcaa gtcagctacc tttatgacct atcgctgaat
     2461 caaaaattaa tgttactcag tcagagttac gctaatcaat tgaccataca ggtgactaga
     2521 aaacagaaaa atggctatca aactattatc ggaaccattc cgactctttc aacaaatgag
     2581 gtcattctaa aaaccaatga cggacatatc gctatcactt tagaagatat tatggctatc
     2641 acattagttg aggaggtcta ccatgaatca gcttgacttt tataaaaacg aattacagat
     2701 ggactacttc agtgaaaatt accgtaaatt tgaaagtgac ttctatcgct actcagcact
     2761 tgatacacca ctgacttttc taacagatga tatcatgttg gcaatggcga aatctgctaa
     2821 aaactatttc aagctcaata aagaaaatgc taaagataat cgcgatcatt atttcatctt
     2881 tgacatagaa aacctaaatg atagtaaatt gacaaaaaaa tatgtgtatc agcgaacaac
     2941 aagcatcaga caaaaagatt aggccttcat cacaatgcct aatctttttt actcattttt
     3001 aggtttcttc gcctgtctaa actgctctat ccgtttttca agttgctcac tcttcagttt
     3061 attcttagct tcagtaatgg tctcatctaa tataggctcc tttggcatag tttcaggaaa
     3121 ctttttttct tcacggatag tttgccttgt agcatcaaaa acgacatcca ataattggtt
     3181 gaccttatgt aagtcggctt cagacattcc tgataagcga tgaatgagac gcttaaaact
     3241 atcttgatta tgcgtttgct ttttgaataa cctcatcttt tctccccata aaaagcagcc
     3301 cttgtgagga ctgctcatac ttacaagtca tcaatggcta ctgaaatggc ttgtaatttc
     3361 ttattttgct tatttttgtc atcaatcaac tggtgtagct cttcgatttt cttctgactt
     3421 tgaagaatgt cctcattatt cttagccatc acatcg
//
LOCUS       DASFSW010000169         3450 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.170, whole genome shotgun sequence.
ACCESSION   DASFSW010000169 DASFSW010000000
VERSION     DASFSW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.170"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..186)
                     /locus_tag="U1099_001953"
     CDS             complement(<1..186)
                     /locus_tag="U1099_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HEM4868235.1"
                     /translation="MYIEMIDETGQVSAQMQEQITELLQFAAEKIGKQNKEMAVTFVD
                     NKRVHEVNLEYRGIDRPT"
     gene            286..1098
                     /locus_tag="U1099_001954"
     CDS             286..1098
                     /locus_tag="U1099_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM4868236.1"
                     /translation="MLQTPKHITAIIESHLDQMTRLEKQIASYFTQLDPSSADLSQEN
                     TIQQLHISPSALTRFAKKCGFSGYREFVFAFQNNQQYLDKHFEKLQRSLTKKVLVDYE
                     EILTATNKLVDEEKLEEIAKLIDSSKRVYFYGIGSSGLVAMEIKSRFMRLGVVCDAIT
                     DENNLIWTTNILDDSCLVIGLSLSGQTEEVMEHLQLAAQKGIPTALLTTKSFPHPTFD
                     QIIPVASVRHLNYGNRISPQIPLLIMLDIIYAYFLAIDKEKKENIFKQTIRE"
     gene            complement(1202..2710)
                     /locus_tag="U1099_001955"
     CDS             complement(1202..2710)
                     /locus_tag="U1099_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM4868237.1"
                     /translation="MNFKKFSQLGQAFMLPISILPVAGLLLGIGGALSNSNAVAQFPV
                     LDQAWLQAVFTIMSTAGSAVFANLALIFAIGVAVGLADADKGTAGLSAGVAYLVYTAT
                     ISGFLTLFADKGASIDTGVLGALAIGVTVATLHNKYRKIELPAFLGFFGGSRFIPIIS
                     SFAAIAIGSIFYIIWPPIQNLLVDFGKAIAEMGSFGTFLYGFGLRLTGAFGLHHTIYP
                     MFWYTSLGGSEVVAGETIQGAQNIFFAQLADANHTGLFTYGTRFFAGRFATMIFGLPA
                     SCLAMYHAIPKENRKKVGSFYSSSALTSLLTGITEPIEFSFLFVAPWLYVVHAFLDGL
                     SFLVADLLQIRIGNSFSGGLIDYLIFGVFQGNDKTNWIYVIPVGLLWAAVYYLVFKFL
                     VTKFHVLVPGMESSDQASHSEKVLSNGSSLRDNAIAIIDALGGADNIENVTACATRLR
                     VSLRNSGLVDKGVIQSIGATAVLDVKGGIQAIFGGKAILYSQEINQILGLEE"
     gene            complement(2738..3439)
                     /locus_tag="U1099_001956"
     CDS             complement(2738..3439)
                     /locus_tag="U1099_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEM4868238.1"
                     /translation="MGKISKEALKEQIKDGIIISCQALPGEPLYREEGGIMPLLVKAA
                     QEAGAVGIRANSVRDIKEIKEVTTLPIIGIIKRDYPPQEPFITATMREVDELAALDIE
                     VIALDCTKRERYDGLDIVDFINQIKEKYPEQLFMADISTFEEGLTAYEAGIDFIGTTL
                     SGYTSYSRQEEGPDIELVDRLCRAGIDVIAEGKIHYPDQVKIIHDLGVAGIVVGGAIT
                     RPKEIAERFISALHK"
BASE COUNT         1095 a          800 c          579 g          976 t
ORIGIN      
        1 tgtcggacgg tcaattcctc gatactccaa gttaacctca tggacacgct tattgtccac
       61 aaaggtcaca gccatttcct tattctgctt gccaattttt tcggcagcaa actggagcaa
      121 ttccgtaatc tgctcctgca tttgagcaga aacctgccct gtttcatcaa tcatttcaat
      181 atacataatc ttacattcct tttacgtttc cattatatca taaaacctat caatgagggg
      241 gttttagtgg tataatgtcc tcagaagcta tcagaggaat ggaaaatgtt acaaacaccg
      301 aaacacatca ctgccatcat tgagtctcat ctggatcaga tgactagact tgaaaagcag
      361 attgccagtt attttactca gttagaccca tctagcgccg atcttagcca agaaaacact
      421 atacagcaac tccacatttc tccatctgct cttacccgtt ttgccaaaaa atgtggattt
      481 tccggttatc gagaatttgt ctttgccttt cagaataacc aacaatactt ggataaacat
      541 tttgaaaaat tacagcggag cctaacaaaa aaagtcttgg tagactatga ggaaatccta
      601 actgctacca ataaactggt agatgaggaa aaattggaag aaattgccaa actgattgac
      661 agtagcaagc gtgtctattt ttacggaatc ggcagttctg gtttggttgc aatggaaatc
      721 aaatcccgct ttatgcgact gggtgtagtc tgtgatgcaa ttactgatga aaacaatctc
      781 atctggacta ccaatattct ggatgatagt tgcctggtta tcggtctttc tctgtctggt
      841 cagacagaag aggtcatgga acatctccag ctggctgctc aaaaggggat tcctaccgcc
      901 ctgttaacaa ctaagagttt tccccatccg acttttgacc aaatcattcc agtcgcctct
      961 gtccgccacc ttaattacgg caatcgtatc tcaccccaaa tccccctgct catcatgcta
     1021 gatattatct acgcctattt tttagctatt gacaaggaga aaaaagagaa tattttcaag
     1081 caaaccatta gagaataatt catccgtctt tctatttcac aacaatctta ataaaacaaa
     1141 aagcattcaa aaatacccga taggtacagt ttgaatgctt ttaattcgct taaatataac
     1201 tctattcttc aagcccaaga atttgattga tttcttggct atacagaata gccttacctc
     1261 cgaaaatagc ctgtattcct ccttttacat ccaaaacagc cgttgcacct atagactgaa
     1321 taactccctt atccacaaga ccactatttc tgagcgatac tcttaatcga gttgcacagg
     1381 cagttacatt ctcaatatta tctgcaccac caagagcatc gatgatagca attgcattat
     1441 cacgaagact agaaccgttt gataaaactt tttcagaatg tgatgcttgg tcactacttt
     1501 ccataccagg aactagcacg tgaaatttgg ttactaaaaa tttgaaaact agataataaa
     1561 ctgctgccca aagcaagcca acaggaatca cgtaaatcca attggttttg tcatttcctt
     1621 ggaaaacacc aaaaatcaag taatcaataa gcccaccaga aaatgagttc ccaatacgaa
     1681 tttgcaggag atctgcgact aagaatgata gtccatctaa aaatgcgtga acaacatata
     1741 gccatggagc aacaaacaag aaagaaaact cgattggctc ggtaattcct gttaataggg
     1801 aagttaatgc actacttgaa taaaaactgc caaccttctt acgattttcc ttaggaattg
     1861 cgtgatacat agccaaacaa gatgctggta gaccaaaaat catcgttgca aatcgaccag
     1921 caaaaaatcg agttccataa gtaaataagc ctgtgtgatt cgcatctgct aattgagcaa
     1981 agaaaatatt ttgcgcacct tggattgtct cacctgctac cacttcactt ccgcctaatg
     2041 aagtatacca aaacattgga tagatggtat ggtgcaagcc aaatgcacct gtcaaacgta
     2101 atccaaaacc atataagaaa gttccaaaac ttcccatctc tgctattgcc ttaccaaaat
     2161 caactaacaa attctgaatc ggtggccaaa ttatataaaa aatggagcca atcgcaattg
     2221 ctgcaaatga tgaaataatt ggaataaagc gagagccccc gaagaaacca agaaaggctg
     2281 gtaattcaat ttttcgatat ttattgtgta aagttgcaac agtgacacca atcgctaaag
     2341 cgcccaaaac tccagtatca atgcttgctc ccttatctgc aaaaagagtt aaaaatccac
     2401 taatagtagc cgtataaacc aagtatgcaa ccccagcaga taggccagca gtccccttat
     2461 cagcatctgc caaacctaca gcaacaccaa ttgcaaaaat caatgctaaa ttcgcaaata
     2521 ccgcactacc tgccgtactc atgatagtga atacagcttg caaccacgct tgatccaata
     2581 cagggaattg tgctacagca ttactatttg ataaggcgcc tccaattccc agtaagagac
     2641 cagcaacagg caaaattgag attggcagca taaaggcctg ccctagctga gaaaacttct
     2701 taaaattcat actccattcc tttctataac ctatttatta tttatgtaaa gcactaataa
     2761 accgctctgc gatttccttt ggacgtgtga ttgcaccgcc tacaacaatt cctgccacac
     2821 ctaaatcatg aataattttt acttggtcag gatagtgtat ttttccttcc gctatcacat
     2881 cgatacctgc tcggcacaag cggtcaacca attcaatatc tggtccctcc tcctgcctac
     2941 tataggatgt ataccccgaa agtgttgtcc caatgaaatc gatgcctgct tcataagcag
     3001 ttaatccttc ctcaaaggtt gaaatatctg ccataaaaag ttgttcgggg tatttttcct
     3061 tgatttgatt aatgaaatca acaatatcga gtccatcata acgctcacgc ttggtacaat
     3121 caagagcaat gacttcaata tctagagctg ctaactcgtc tacttctcgc atcgtagccg
     3181 taataaaggg ctcttgtgga ggatagtctc gcttgataat tccgataatt ggtaaagtag
     3241 ttacttcttt tatctcctta atatctcgta cactattggc ccgaataccg acagcccctg
     3301 cttcttgggc agccttgact agtaaaggca taataccgcc ttcttcccta tacagaggtt
     3361 cgcctggcaa agcctgacag gaaatgataa ttccatcttt tatctgttct tttagagctt
     3421 ccttactgat cttgcccatt aatttttcct
//
LOCUS       DASFSW010000170         3427 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.171, whole genome shotgun sequence.
ACCESSION   DASFSW010000170 DASFSW010000000
VERSION     DASFSW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.171"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            221..625
                     /locus_tag="U1099_001957"
     CDS             221..625
                     /locus_tag="U1099_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4868239.1"
                     /translation="MKYYIHVRGSKIPVTETVYKSYWQLVNHEKYLNRKDRKFGVLPF
                     SSFEVDGLLLANVLPDTTVDVERFIENKLLIEQLNEALLTLSDKEFKIIDALYFREHT
                     IREVAIEHEMSATSLFRMRNTILDNLRKFFEK"
     gene            1130..2872
                     /locus_tag="U1099_001958"
                     /pseudo
     CDS             1130..2872
                     /locus_tag="U1099_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2996..3249)
                     /locus_tag="U1099_001959"
                     /pseudo
     CDS             complement(2996..3249)
                     /locus_tag="U1099_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         1259 a          488 c          704 g          976 t
ORIGIN      
        1 attgttacct actaaacaga aacttattaa aatatagatc agcaagttca gaagagcttg
       61 ctgttttttg gtacatataa ttttttttgg aacaagagca ccatattttc actcttatag
      121 tgaggagaac tccttgattt gataaaagtg agaaatagct atggttgaga taaggagccc
      181 tccttttatg actaagaaaa aatcataaac ggaggctgaa atgaaatatt atattcacgt
      241 tagaggtagt aaaatccccg taacagaaac ggtttataaa agctactggc aattggtcaa
      301 tcacgagaag tatctgaata gaaaagatag gaaatttgga gtattacctt tttcatcatt
      361 tgaagtagat gggttactgc tagcaaatgt actgcctgat acaactgttg atgttgaacg
      421 ttttatagaa aacaagctcc ttattgagca actaaatgag gctttattaa ccttatctga
      481 taaagagttt aaaatcattg atgctctgta ttttagagag cataccatca gagaagtagc
      541 aatagagcat gagatgtctg cgactagtct atttcggatg agaaatacaa ttttggacaa
      601 cctacgaaaa ttttttgaaa aataaggaaa caggagacta aaaaattcac tctttatagt
      661 gagaggaatt tttagtctct ttttgccctt tgacaattga ataattctag gtgggacttt
      721 atttatttgt tgagcaatag aatgtaatgc gccaggaaat gagcggaaat catttattca
      781 aaaataggat ggtgacctat ctgccatgac acaaataatg ataatgatac ttctgaccgt
      841 tcaggctgcc aacgtaaaag gacagcgggt gaaattccca tgattgatat aaccagtcat
      901 acccacagag aataactaag agtaacatgt tatcaggagg gtaagttttg gaacaatcag
      961 tagtaaaata tagtatgcaa ctttcaatgc ttaatttttt atattccaga aatatgttga
     1021 ctaaaaagga atatgaaaaa attaaaaata gactgcgcat taagtacaca aattagctga
     1081 ccttttgatt tgaacgcggt aatatagaat caaaaggagg aatttcagta tgaaaaaaaa
     1141 taatgttgaa ataataaaag ctgattcttt agttcgtcgt agaggcgaca atttcgaaag
     1201 agagtcaaaa agggttgctg cttattgccg agttagttct gatagcgaag atcagaagaa
     1261 cagctatgaa tctcaggtga gacattataa agattatatc tctcagcggt cggattggga
     1321 actggcaggt atctatgcag atgaggggat ttcaggaaca caagtaggaa agagacaaga
     1381 ttttcaacga ttaataaatg attgtgtaaa tggagaaata gattacattg tcacaaaggc
     1441 aattgctaga tttgccagga ataccctaga tactttgaaa tatgtcagaa tgttaaaaga
     1501 tatgcaaatt ggagtatatt ttgaggaaga aaatatagat accctaacaa tggatggaga
     1561 attgcttttg actattctaa gctccgttgc acagcaagaa gtagaaaata catctgctca
     1621 tgtgaaaaaa ggattaaaaa tgaagatgca acgtggtgaa ttgatagggt ttcaaggatg
     1681 cttggggtat gactatgatg tagaaaccaa gcaactttca atcaataaaa aagaagccaa
     1741 aatagttcgc tatatctttg aaaggtactt agaaggtatt ggaggaaaag taattgcaag
     1801 ggaacttgat gaactaggtt acaaatcgcc gagaggatta gatcattgga atgatacaac
     1861 agtcttagga atcataaaga atgaaaaata taaaggcgat attctaatgg gcaaaacttt
     1921 tacagtggat ccaataagca agagacgttt aagtaatttt ggtgaagaag ataagtacta
     1981 catcaaagat aatcatgaac ctataatatc taaagaagac tttgagaaag ctcaggagat
     2041 aagacttcgt cgagcaggaa ataagaagac tgctgcaaat gtaaatggca agcgtgaacg
     2101 ctattcaaaa atgtatgctt ttagtagcat gttagaatgt ggtttttgtg gttcaatatt
     2161 atctagaaga agttggcact gccgttcaga ttatcgcaag gttgtgtggc actgtgttac
     2221 atcgataaag aaaggaaaga aattttgtaa gcatagtaaa ggattagaag agctcgctat
     2281 tgagggggct ttcatggaag cttacagaaa actttatcat tcaaatgaaa acttaatgac
     2341 agacttactt gaaacgattg aatctgaatt gaatgacaat agccttaata aggaactaaa
     2401 aaggattaca aacaaacttc gaacattact aaaaaaagag gaaaatcttg taaatctaag
     2461 gctcgaaggg aaaattagcg ataccatata caatgaaaag tacaatgaaa tttcctcaga
     2521 aaaagagttt ctagcagaag agaaggtaaa tattgaaaca accttaaaat cagaaatcga
     2581 tgtaaagaaa agactaactg agtttaagca cttgctatct tcacagaaaa tgcttacgga
     2641 gtttgatcgt gcagtttttg aaagtattgt tgagaaaatt attgtaggtg gagttaatag
     2701 cgacggagaa attgatcctg caatgttaac cataatattc aaaacaggtg aaactcaaaa
     2761 caaggatggt aaacaattta aaagtaaacg taaaaatgct aaactagaaa cagataaatt
     2821 gtgtcctcaa aacagtgacg aggataaaaa attgtattct caaggaacag actacacaca
     2881 tagaagttgt gggattatta gaaaaacgca attagattgt ccagtatgct ctcctgtttt
     2941 ttggcaagca gcttcaaagg gccccaggac ctttgaactc tcaaatacgc atcatgttga
     3001 cagttgccac acctacacca aaatcaaatg ccaacaagcg ttgagtcggg taatagcgta
     3061 agtagcgcaa ggtcatgata agctgctctt ccatacttag acggcgtggg cgtcctcctt
     3121 ttcggtgttg ctcttgataa gcgtcagtga gacaatcaag catcagatga aacgtcgctt
     3181 ttttacacct atcaacaatt tgaaattctc tgagtttaat tttaagactt tttcgtatgt
     3241 tgtttccata cctttagtat accgcctttg agttaccgaa caagtctatt gctaaacttg
     3301 atgaaggttg tattgtctgt tataatattg gatattgcaa gctttaagtg aaaggtttta
     3361 gggaagagat tatcacaact cattctttat tagttatgag acgatggtat taatgatagt
     3421 ctagtaa
//
LOCUS       DASFSW010000171         3395 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.172, whole genome shotgun sequence.
ACCESSION   DASFSW010000171 DASFSW010000000
VERSION     DASFSW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.172"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(81..695)
                     /locus_tag="U1099_001960"
     CDS             complement(81..695)
                     /locus_tag="U1099_001960"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM4868240.1"
                     /translation="MSVIERLTKAAHLIDMDDIIREGHPTLRQVAEEVAFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMKLRGGVGLAAPQIDVSKRIIAVLVPNPEDEEGNPPAQ
                     AYSLQEVMYNPKIVAHSVQEAAMEGGEGCLSVDREVQGYVVRHARVTVDYMDKNGEKH
                     RIKLKGFNAIVVQHEIDHLNGIMFYDRIDPEHPFAVKEGMLVIE"
     gene            825..1988
                     /locus_tag="U1099_001961"
     CDS             825..1988
                     /locus_tag="U1099_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM4868241.1"
                     /translation="MKRILEKASLLALSTMLVSTFAVSPAIPQMIEHFAQQGIAAAQV
                     ENLITVTSFAIMVTLLANGLVVRFLSERNIIIAGLLLMAIGGALPMFLTAYPLILLAR
                     ILLGLGIGLINARAINIIGNFFTGQERVQMMGLRGSAEVLGSAGLTLLVGWLTQFGWQ
                     PAFLVYLFALVILALFLLFVPKEELLARMEVKVEENAPKVKLDKTMWMMGIYLAFLAF
                     FVINVNTFLTIRIPQIVLAKGIGTAQQASLILSLMQVMGIVAGTVFSSLVGRLKGWLL
                     AVSYVVFGLAVVGIAFADNLWVLGLGGMVSGFFYSIILTIVFSQVTDRAPKALLNAVM
                     TIVLMGCNIGGATSAILPTYLEKLNPTPTGAFGIYAIGCALISAGLIYKQLKK"
     gene            2025..2195
                     /locus_tag="U1099_001962"
                     /pseudo
     CDS             2025..2195
                     /locus_tag="U1099_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            2202..2651
                     /locus_tag="U1099_001963"
     CDS             2202..2651
                     /locus_tag="U1099_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4868242.1"
                     /translation="MDYFYIMPLPQDLALEIANQWRYQPPLDAYTISANPETYAEMIS
                     PEARGDRFFAVIRNAALMGYFCIDQDGETVDIRLGMKPSLTGQGNGRAFYQAIEDYLV
                     EHVQPASIKLTVASSHQVAQKLFHALGFTEIEKQSEYIKMEKKLVCD"
     gene            2642..3370
                     /locus_tag="U1099_001964"
     CDS             2642..3370
                     /locus_tag="U1099_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM4868243.1"
                     /translation="MRLDKFLVDCGVGSRTEVKKLLKNKQVTVNGQVETSPKQQIDEK
                     SDQIAVAGQVLSHETFVYYVLNKPKGVISATEDDRHRTVLDLLDNTARQKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVMLSPKKHVDKLYRAQVDGIMTQEDVERFAAGIELKDLTC
                     QPAQLTILSTDEAKQTSLVDITIREGKFHQVKRMVQACGKTVTDLQRLTMGPLRLDPK
                     LALGEYRRLTAAELKQLEVFGVEL"
BASE COUNT          866 a          735 c          807 g          987 t
ORIGIN      
        1 attgagatcg atccgaaagg gtcgtttttt cgtactcaca taaaaaactg gctgaaaatt
       61 tttcagtcag tttcttatgt ttattcaatt accaacatgc cttctttaac ggcaaatggg
      121 tgttctgggt caatgcggtc gtagaacatg ataccgttga ggtggtcgat ttcgtgttgg
      181 accacgatgg cattaaagcc tttgagtttg atacggtgct tttctccgtt tttgtccatg
      241 taatcaacag tcacgcgcgc atggcggaca acgtaacctt ggacttcgcg gtcaacggat
      301 aggcagccct cgcccccttc catagcagct tcttggacag aatgtgccac gattttaggg
      361 ttgtacatga cctcttggag ggagtaggct tgggcaggtg gattgccttc ctcatcttct
      421 ggatttggga ctaagacagc aatgatgcgt ttggaaacgt caatctgagg tgcagctaga
      481 ccaactccgc cacggagttt cattttttca gccatgacag gatcctgcga atgtttgagg
      541 aactgcatca ttttttcgcc taaaataatt tcctgatcag atagtggaaa tgcaacttct
      601 tcggcaactt ggcgtagggt tggatgccct tcacggatga tgtcatccat gtcaatcaaa
      661 tgggcagctt ttgtcaatcg ttcaataaca gacatgattc tctcctttga atatagttaa
      721 gataagtata gcatgaaatg ggagagaata aaagtcggac ttttctatcc attcgctttc
      781 aaatctgtta tactagtaaa agacgaacgt tgaaagggag aaaaatgaaa agaattttag
      841 aaaaagccag tttgttggcc ctatccacca tgctggtttc gacatttgct gtctcgccag
      901 ctattccaca gatgattgag cattttgccc agcagggaat tgcagcggcc caggtggaaa
      961 acttaattac ggtgacctcc tttgctatta tggttactct attggcaaat ggtctagttg
     1021 tccgtttctt gtctgaacga aacattatca ttgcagggct tttgcttatg gccatcggtg
     1081 gagctttgcc catgtttcta actgcttatc ctctgatttt attggctcgc atcttgcttg
     1141 gtttggggat tggcttaatc aatgcgcgtg ccattaacat catcggtaat tttttcaccg
     1201 gtcaggagcg ggtacagatg atgggcttgc gtggttcagc agaggttttg ggaagtgcag
     1261 ggctcaccct ccttgtcggt tggttgaccc agtttggttg gcaacctgcc ttcttggtct
     1321 acctttttgc cttggtcatc ttggctctct tccttctctt tgttcccaag gaggaattgc
     1381 tggctcgcat ggaggtaaag gttgaggaga acgcgcccaa ggtcaaactg gacaagacca
     1441 tgtggatgat gggaatttat ctggcattct tagccttctt tgttatcaac gtcaatacct
     1501 ttctgaccat tcgtattcct cagattgttt tagccaaggg catcggtact gctcagcaag
     1561 ccagtctcat tctcagtctt atgcaggtca tggggattgt ggcagggacg gtctttagta
     1621 gcctggttgg tcgtttgaag ggttggcttt tagctgtttc ctacgtggtc tttggactcg
     1681 cggttgtcgg cattgccttt gcggataatc tctgggtgct gggactcggt ggcatggtgt
     1741 caggattttt ctacagcatc attctaacca ttgtctttag tcaggtaaca gaccgtgcac
     1801 ccaaggcctt gctcaatgcg gtcatgacaa tcgtccttat gggctgcaat atcggcggtg
     1861 cgacatctgc tatcttaccg acttatctgg aaaaactcaa cccaacccca acaggtgcct
     1921 ttggtattta cgccatcggc tgtgccttga taagcgcagg cttgatttat aaacaactga
     1981 agaagtagga gcggatttcc gctccttttt ggtataatat tcttatgtta gaaaataacc
     2041 gcttaaatga attaatacta cgttttcccg aggatgttca gttgattttt aaggacatga
     2101 ttccctccta tcagttgggc tatgtggatt atgtttatca gacagatagt tacgccaccc
     2161 aaaaccgtcg gattaaaaat ttggcaaaaa atttccgaag aatggattac ttttacatca
     2221 tgcccctgcc ccaggatctg gcattggaaa ttgctaacca atggcgctat caaccacctt
     2281 tggatgccta tacgattagt gcaaaccccg agacttatgc tgagatgatc tcaccagagg
     2341 cgcgtgggga ccgctttttt gcagtcatcc gcaatgccgc cctcatgggc tatttctgta
     2401 ttgaccaaga tggcgagacc gttgacattc gtttgggtat gaaaccttct ttgacaggtc
     2461 agggaaatgg cagagctttc tatcaggcta ttgaagacta tttagtagag catgttcagc
     2521 cagctagtat caaactgaca gttgctagct ctcatcaagt tgctcaaaaa ctctttcatg
     2581 ctcttggttt tacagaaata gaaaagcaat cagaatatat caaaatggaa aagaaactcg
     2641 tatgcgatta gataaatttt tagtggattg cggtgttggc agtcggacag aagtcaagaa
     2701 actcctgaaa aacaagcaag tcacagtcaa cggccaagta gaaacctccc caaaacagca
     2761 gattgatgaa aagtcagatc agattgcagt agcaggtcag gtcttgagtc atgaaacctt
     2821 tgtttattat gtattgaaca aacccaaggg agttatttca gcaaccgagg atgaccgtca
     2881 tcggactgtc ttggacctgc tagataatac agctcgccag aaggaagttt ttccagttgg
     2941 acgcttggac attgataccc atggcttgct cctcttaacc aacaacggtc agctggctca
     3001 tgtcatgctt tctccgaaaa aacatgttga taaactctac cgtgcacagg tggacgggat
     3061 catgacccaa gaggatgtcg aacgatttgc ggcaggaatt gagctgaaag acttaacctg
     3121 tcaaccagcc cagttgacca tcctctctac agatgaagct aagcagacct cgctcgttga
     3181 catcaccatc cgagaaggga aatttcacca ggtcaaacgc atggtgcagg cctgtggaaa
     3241 gacggtgaca gacttgcaac gattgaccat ggggcctctt cgcttagatc caaaattagc
     3301 gcttggagaa taccgcaggt tgactgctgc cgaattaaag caattagaag tctttggtgt
     3361 ggaattatag ataaatagaa gagacttgct cccga
//
LOCUS       DASFSW010000172         3382 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.173, whole genome shotgun sequence.
ACCESSION   DASFSW010000172 DASFSW010000000
VERSION     DASFSW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.173"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..415)
                     /locus_tag="U1099_001965"
     CDS             complement(<1..415)
                     /locus_tag="U1099_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEM4868244.1"
                     /translation="MEIARKNFIIVAAMISFISVTLLGLLYYAMPIYYNQVKKQELRH
                     DYMTVAKQLDGMPEAKIISEIDDFDIKTPNIIISLFSSDRKIIYPDPNDEMAKKHENE
                     YLENGDFDEIGSWGAEITSAEGVTYYLLFNYGFHSL"
     gene            complement(417..1067)
                     /locus_tag="U1099_001966"
     CDS             complement(417..1067)
                     /locus_tag="U1099_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4868245.1"
                     /translation="MSRILVVEDDIVISQVVCEFLKEHGYQVESVFDGKVALERFQEE
                     QFDLIVLDIMIPSMTGLEVLKEIRKTSQIPILMLTAMGDEYTQLISFNQIISDYVVKP
                     FSPTILVKRIENILRGKGETDSIEIGTILIQPTSGAVYMEEEEVQLTKKEYEVLLYLA
                     KRRGKIVSRDNLMMGIWGYTELDSRVLDNHIKNIRKKLPSLPLKTIVGRGYQIEGT"
     gene            1299..2000
                     /locus_tag="U1099_001967"
     CDS             1299..2000
                     /locus_tag="U1099_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4868246.1"
                     /translation="MWNPKKLTVLKWFDILILTIILFGDGIINSTLQYIALQNQTTTL
                     QENLTFSTMDNYKALALQLVWLTIAIFYLLLRNFDFSIWKKHIFITPWVPLQAVALFI
                     FSALCLDIYNLVSYQFLASNTPSMFQLFPNIDLSLILYSLLNGFYEEIFFLNLCLLVN
                     PKYAKWAFLYSLIIRCSFHTYQGLISALGLGLILGTIFYLLYQKIKPKNLLPFFLAHA
                     AADIIGLTVLSYILY"
     gene            complement(2467..>3382)
                     /locus_tag="U1099_001968"
     CDS             complement(2467..>3382)
                     /locus_tag="U1099_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4868247.1"
                     /translation="PKVSIKKNSSTFWLSWITSSKTTITWNLDKEDLIKAYQTNQPLS
                     LTYQLQLKDKKKKDSQKLVSTEISHEINDKVGKKVYSSLKVSLNHKTKKVSVPVPIEP
                     QVPDKPIDMPKVENETPKGTPETPMQEKPNVPEATLPMQPESPKETGKPGNNVEPESL
                     KPIEDSTDDSANKPDSTIPKVTDTPDLSRPMKPESTEEIDKAENSDTPDSSKTIKDSD
                     NSAPISTIKDKVNAETVANQVELAMNQKSRLSENMEKVSSVNRSNEKSHTLPSTGQAD
                     SPIYTLTALSLLVGAQVLYRTKRKQESE"
BASE COUNT         1025 a          653 c          497 g         1207 t
ORIGIN      
        1 ataacgagtg aaatccataa ttaaataaca gataatatgt aactccttct gcagatgtta
       61 tctcggctcc ccaagaacca atttcatcaa aatccccatt ttctaaatac tcgttctcat
      121 gctttttagc catttcatca ttggggtctg gataaataat ttttctatca ctcgaaaaaa
      181 gactaataat aatattcgga gtttttatgt cgaaatcatc aatctcagat ataatcttag
      241 cttctggcat cccatccaat tgtttagcca cagtcatata atcgtgtctc aattcctgtt
      301 tcttaacctg attataatat ataggcatag cataatacaa taaccctaac aatgttacag
      361 agatgaaaga aatcatcgca gcaacaataa taaaattttt tctggcaatc tccatattaa
      421 gtaccctcta tttgataacc acgaccaact attgtcttca aaggaagtga cggcaatttc
      481 ttacggatat tcttgatatg attatctaag acacgactat ccaattccgt atatccccat
      541 attcccatca tcaagttatc acgactaaca attttcccac gtcgtttagc taaatatagt
      601 aaaacttcat attctttttt cgtcaattga acttcttctt cttccatata aactgccccg
      661 ctagttggtt gaataagaat tgttcctatc tcaatgctat ctgtctctcc ttttcctctc
      721 aaaatattct caatccgttt taccaatata gttggtgaaa atggtttaac aacataatca
      781 cttataatct gattaaaact aataagctgt gtatattcgt cacccatggc tgtcagcatc
      841 aaaattggaa tctgagaagt tttgcgaatt tccttcagta cttccaaacc tgtcatagat
      901 ggaatcatga tatctaaaac aattaaatcg aattgttctt cttgaaacct ttctaaagca
      961 acctttccat caaaaacaga ttctacctga taaccatgtt cttttaaaaa ctcacaaaca
     1021 acttgactaa taactatgtc atcctctaca accaaaatcc ttgacatttt tatacctcct
     1081 cgtaaagttc tattataaaa tattattata cttcaattat ataccgattc gtacaatggt
     1141 gcaactctat catagataaa tagagcacca tgttctgcta tttcagcttt cttttgactt
     1201 cttttgttta aaacagataa aaaacagacg aaaccgagag catatataga tttgtttgat
     1261 aaactgaact aaacaaaaat gaaaggaact ctcccattat gtggaatcca aaaaaattaa
     1321 ccgtattaaa atggtttgat attctcattc taacaattat tttatttgga gatggaatca
     1381 tcaactctac tctccaatac atagccttac aaaatcaaac tacaactctt caagaaaatt
     1441 tgacattttc aacaatggat aattataagg ctttggctct acagttagtt tggctcacaa
     1501 tagccatctt ctatctatta ctccgaaatt ttgatttctc aatttggaaa aaacatattt
     1561 ttattactcc ttgggttcca ttacaagctg ttgccttatt cattttttct gcattatgct
     1621 tggatatcta taatctagta tcctatcagt ttctagcttc caacactcca tccatgtttc
     1681 aactatttcc taacattgat ttatcactga tcttgtactc attgttgaac ggattttatg
     1741 aagaaatttt cttccttaac ttgtgcttac tggttaaccc taaatatgca aaatgggcat
     1801 ttttatattc gttaattatt cgatgtagtt tccataccta tcaaggttta attagtgcac
     1861 tcggtttagg cttaattcta ggaactattt tctatctact ttatcaaaaa ataaaaccta
     1921 aaaatttatt accatttttc ctagcacatg ctgcagctga tattatcggt ttaactgttc
     1981 tatcatatat tctatactag ttatctaaca gataatttga acacaagcta tcctcatatc
     2041 accaaagact gagtggaccc ctactagtca tgaccacccg tgaaaacggg tggtttattt
     2101 tgtgtaattt agagaaaaga gtgccaaaaa acgcataaaa tcgtgttgct aagaacgttg
     2161 attttatgcg ttttattata tgacttatct cgatattttt attgaaattt atgtttatgc
     2221 ccaactatac ttctcagaat caatgtccat atctcatctc agcctagtat agattgacag
     2281 atttattcta cattcccagt tcagcctctg cagctattgc gaatggctcg ttttatattc
     2341 aatctccaaa gatttcctaa aacttggatc tgtacggagg gtaaaacaat tcgatacact
     2401 attataggca taatctctac tctattaacc atatgtatat acaagtagaa attacacgac
     2461 caacatttac tccgattctt gcttacgttt tgtacgataa agtacctgag ctcctacaag
     2521 aagagagagg gcagttaacg tatagattgg agaatcagct tgtccagtag atggaagggt
     2581 atgtgatttc tcgtttgaac gattcacgct agaaactttc tccatatttt cagaaagacg
     2641 tgatttctga ttcatagcaa gctctacctg atttgccacc gtttcggcat tcactttatc
     2701 tttgatagta gaaattggag cagagttatc tgaatcttta atagtcttag aagaatctgg
     2761 agtatcacta ttttctgctt tatcaatctc ttcagttgac tctggtttca ttgggcgaga
     2821 caaatctggg gtatccgtta cttttggaat tgttgagtct ggtttatttg ctgaatcatc
     2881 ggttgagtct tcgataggct taagtgattc tggctcaaca ttattgcctg gttttccggt
     2941 ctctttaggt gactctggtt gcattggaag agttgcttct ggaacatttg gtttttcctg
     3001 cataggtgtt tcaggagtcc ccttaggcgt ttcattctct acttttggca tatcaattgg
     3061 cttgtcagga acttgtggct caataggaac aggaaccgaa actttcttag ttttatggtt
     3121 aagagacact tttagggatg agtatacttt cttcccaact ttatcattaa tctcatggct
     3181 aatctctgta cttacaagtt tttgggaatc tttcttcttt ttatctttca gttgtaactg
     3241 ataagttaaa gaaagtggtt gattagtctg ataagccttg attaagtctt ctttatctaa
     3301 gttccaagta atagttgttt tcgagctagt aatccatgac agccagaacg ttgaagaatt
     3361 ctttttaata cttactttag gg
//
LOCUS       DASFSW010000173         3363 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.174, whole genome shotgun sequence.
ACCESSION   DASFSW010000173 DASFSW010000000
VERSION     DASFSW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.174"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..329
                     /locus_tag="U1099_001969"
     CDS             <1..329
                     /locus_tag="U1099_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868248.1"
                     /translation="KQLPPHLHKVFRLLPLGMDLPITGTDIEKLTGLDVRTIREHIRQ
                     LIVDYGIPVCGGRDNKQGGYYIPQNETERLAGVLPLQRQYDQEHKRIHALLTADLQDW
                     RKYRDV"
     gene            322..603
                     /locus_tag="U1099_001970"
     CDS             322..603
                     /locus_tag="U1099_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM4868249.1"
                     /translation="MFSLSRESEQDLTNGLLDMVGKYLEAREMVKPRVTGLITAQQVK
                     DELDIEYKTLKRWEDNGLRRYQPPIEDTKKIFYRISDILIFLGVENGKV"
     gene            590..736
                     /locus_tag="U1099_001971"
     CDS             590..736
                     /locus_tag="U1099_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868250.1"
                     /translation="MARYSIHPADSGGHYHDIKLYKDRRPTFEQVKEQQRLKKLKKKR
                     RKGR"
     gene            738..1616
                     /locus_tag="U1099_001972"
     CDS             738..1616
                     /locus_tag="U1099_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEM4868251.1"
                     /translation="MAIYEARGFQNNLVYPFDRLEPFEYIAQFRPMKVPEGVDIEQFK
                     RTQAPYCISGKVTADKKGTCRRNNNSLVYRDLIFLDYDELEASVDFPRIVSEALSDYS
                     YIIYPTIKHTAEKPRYRLVVKPSHKMKETTYRATVQEIADIIGLPFDMASLTWSQLQG
                     LPVTTGDPEEYQKIIHRGSDYPIQAVLDKQPTKRVVTTTYTPRPNGQRSITMRVIDTL
                     FNGFGDEGGRNVAVTRFVGLLVGKWVNCDISTAWELTQIANSVTADPLPVNELEATFE
                     SIVKTEIRKRGLGISN"
     gene            1670..3106
                     /locus_tag="U1099_001973"
     CDS             1670..3106
                     /locus_tag="U1099_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM4868252.1"
                     /translation="MKELYNTLYELGAIWREEHQYTVNEGKKNEKIVIPIPEVSTVAK
                     YLRQVCHFAFISVGDSSDKSPLYLYHYDKGIYTDSTDLFNKLCAKFDSRLKPRAWADI
                     RAFIRTTTRISKPFSDHTRIPVANGVFNLKTKQLEPFSPDFVITSKIRTAYNGAARKP
                     ILDGWFDFEQWLDIIACGDSEIYALLWQIMNEAINPNRTRGKLAILLGDGNNGKGTFQ
                     SLLMNLIGADKVATLRPDQFEGHNLASLSGKVCNIGDDISNRYIDEVSDLMSIVTGDT
                     VTINPKHQQPFELSLKLFCLFSANELPRVRNKSQGWYRRLCIVPFKADFNGQKERPEI
                     KNIFLKDTELLEWVLFKILNMPAFDKFIEPEAVAKEIDSYKKENDYLYAFVTDDYTER
                     ELHLIERVPLKWIKEEYRTFLAENDLSAHIPYSFGKDLVRILRVHTGGKYSLRQGRLK
                     KKEAEVFPYPLTIAEYTEYPLRLVEKDE"
BASE COUNT         1093 a          644 c          794 g          832 t
ORIGIN      
        1 aaaaacaact accaccacac ttacacaaag ttttcaggct cctaccgctt ggaatggact
       61 tacctatcac ggggacagat attgagaagc tgacaggctt ggatgttcga accattcggg
      121 agcatatccg ccagctaata gttgactatg gcatacccgt ttgtggcggg cgtgataaca
      181 agcaaggggg ctactatatc ccccagaatg aaactgagcg acttgctgga gtgctaccgc
      241 tccaacgtca atacgaccag gaacataagc gtattcacgc gctactgact gctgatttac
      301 aagactggag gaagtataga gatgtttagc ctaagcagag agagcgagca agatctaact
      361 aatggcttgc tggatatggt tggaaagtac ctggaggcgc gtgagatggt caagccaagg
      421 gttacagggc taattacagc ccagcaagtc aaagatgaac tagacataga atacaagact
      481 ttgaagcggt gggaggataa cgggctgaga cgataccaac caccgataga ggacactaag
      541 aaaatatttt ataggattag tgatattttg atatttttgg gggtggagaa tggcaaggta
      601 tagcatacac ccagcggata gtggcggaca ctatcacgat attaaattat acaaagaccg
      661 ccgaccgact tttgagcagg tcaaggaaca gcaaagactt aaaaagctga agaagaaacg
      721 tagaaagggg cgataatgtg gcaatttacg aggctagggg ctttcagaat aatctagtct
      781 atccgtttga taggttggaa ccgtttgagt atatagcaca gtttagacct atgaaagtac
      841 ctgagggtgt ggatattgag caattcaaac gcacacaagc cccctactgt atcagtggaa
      901 aagtcacagc agataaaaaa gggacttgca gacgaaacaa caacagccta gtctatcgtg
      961 atttgatttt cttggactat gacgagctag aagcaagcgt agactttccc agaatcgttt
     1021 ctgaggcact ttctgactac tcctatatca tttacccgac tatcaaacac acggctgaga
     1081 agccccgtta caggcttgta gtgaaaccta gtcacaagat gaaagaaacg acctacaggg
     1141 caacagtgca agagatagcg gacataatcg gactaccctt tgatatggct agtttaacct
     1201 ggtcacaatt acagggttta cccgtgacga ctggagatcc agaagaatat cagaaaatca
     1261 ttcatagggg gagcgattac cctattcaag cggtattgga taaacagccg acaaaaaggg
     1321 ttgtaactac tacttacacc ccacgaccaa acggtcagcg atctattact atgagggtta
     1381 ttgatacgct tttcaatggc tttggagatg aaggcggtag gaatgtggca gttacccgat
     1441 tcgtgggctt attggttggc aaatgggtta attgtgacat ctcaacagcg tgggagctta
     1501 cccagatagc aaacagcgtg acggctgatc cgttgccagt taacgagcta gaagccacgt
     1561 ttgaaagcat agtaaaaaca gaaatcagaa agagagggtt aggcatatcg aactagaaca
     1621 attacaggca gaaatggagc aggtcagcac catttcagag cctacaagca tgaaagaact
     1681 ttataacaca ttatacgaac ttggcgcaat ctggagagaa gaacatcagt acacagtcaa
     1741 cgagggcaag aagaatgaaa aaatagtaat tccaattcca gaagttagta ccgtagctaa
     1801 gtatttgcga caggtttgtc actttgcttt tataagcgtt ggggatagct cggacaagtc
     1861 accgctatac ctgtatcact acgacaaggg aatatatacc gatagcacgg acttattcaa
     1921 caaactatgt gctaagtttg acagcaggtt aaaacctaga gcatgggcag acattcgggc
     1981 gttcattcgg acaactaccc gaatttccaa acctttcagc gaccacacac gcatacccgt
     2041 agcaaacggg gtgtttaatc tcaagacaaa gcaattagaa ccatttagcc ctgattttgt
     2101 tatcacaagt aagattagaa cggcatacaa tggagcagca agaaaaccaa tactagatgg
     2161 ctggtttgac tttgaacaat ggctagacat tattgcatgc ggtgacagtg aaatatacgc
     2221 tttattgtgg cagatcatga atgaagctat caacccaaat aggacacggg gaaaacttgc
     2281 aattctatta ggtgacggta acaatggtaa gggcacattt cagagtctac tgatgaacct
     2341 gataggagcg gacaaggtgg caaccttacg ccccgaccaa ttcgaggggc acaacctagc
     2401 cagtctatcg ggtaaagttt gtaatatcgg tgatgatatt tctaacagat acatcgatga
     2461 agtttctgat ttgatgagta tagtcacagg cgacaccgtg acaattaacc cgaaacacca
     2521 acagccgttt gaactgagct taaaactatt ctgtttgttt tctgctaacg agttgccaag
     2581 agttcggaac aagtcacaag gctggtatag acggctttgt attgtaccct ttaaggctga
     2641 ttttaatggc cagaaagaac ggccagagat taaaaacata ttcttgaaag atacagagct
     2701 attagagtgg gtactattta aaatcttgaa tatgccagcg tttgacaagt ttattgaacc
     2761 cgaagcagtg gccaaagaga ttgacagcta caagaaagaa aatgactact tatacgcgtt
     2821 tgtaacggac gactacaccg aacgggaatt acatttgatt gagcgtgtgc cgttgaagtg
     2881 gataaaagaa gaatatcgga cttttttagc tgagaatgac ctatcggcac atattccgta
     2941 cagtttcggg aaagaccttg taagaatctt gagagtacac acaggcggta agtattcact
     3001 cagacaaggc agattgaaga aaaaagaagc tgaagttttt ccctatccgc tgaccattgc
     3061 agagtacacc gaataccctc tacggttggt tgaaaaagat gagtagattt tgtagtcgtt
     3121 gtagtcgttg tgtagccgtt gtttttttgg agaacgacta cagaatttat tcaatcatat
     3181 caagggttta cgtgttgttt gtagtcgttg tagtcgttgt tttttaagta tgtatatagg
     3241 aaagtaaaaa aaagagctat atataaaaaa taaaactttt tttgaaaaga acgactacat
     3301 tcactacaaa gtggctaaac ccttacggag caagggtttg acagtgtagt tattcttttt
     3361 tta
//
LOCUS       DASFSW010000174         3336 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.175, whole genome shotgun sequence.
ACCESSION   DASFSW010000174 DASFSW010000000
VERSION     DASFSW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.175"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(1015..1863)
                     /locus_tag="U1099_001974"
     CDS             complement(1015..1863)
                     /locus_tag="U1099_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEM4868253.1"
                     /translation="MTVIDGKALGVKLQAALAEKTARLKEEKGVVPGLVVILVGENPA
                     SQVYVRNKERSALAAGFKSEVVRVPDTISESDLLDLIERYNQDDEWHGILVQLPLPAH
                     ISEEKVLLAIDPDKDVDGFHPTNMGKFWSGHPVMIPSTPAGIMEMFKEYQIELEGKSA
                     LVIGRSNIVGKPMAQLLLDANATVTIAHSRTKNLPELARQADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDENGKLIGDVKYDEVSEVASYITPVPGGVGPMTITMLMEQTYEA
                     CVRSAK"
     gene            complement(1993..2727)
                     /locus_tag="U1099_001975"
     CDS             complement(1993..2727)
                     /locus_tag="U1099_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM4868254.1"
                     /translation="MSNAIISIKDLHKYFGKNEVLKGIDLDIQQGQVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGVDITDKSNDIFKMREKMGMVFQQFNLFPNMTVLDNI
                     TLSPIKTKGIAKDEAEKKAKELLEKVGLPDKTNAYPQSLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDGGII
                     VEDGTPEEVFEHTKEERTKDFLSKVL"
     gene            complement(2720..>3336)
                     /locus_tag="U1099_001976"
     CDS             complement(2720..>3336)
                     /locus_tag="U1099_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM4868255.1"
                     /translation="FSVFFGSILGVLLAFAQRSKYKVLAWTANIYVWIFRGTPMIVQI
                     MIAFAMTHFVAPTFQLGILTVDLTRLIPGIIVISMNSGAYVSETVRAGINAVPKGQLE
                     AAYSLGIRPKQAMRYVIMPQAIKNILPALGNEFVTIVKDSSLLSTIGVMELWNGAQTV
                     QSTSYIPLTPLVFAASYYLIMTTVLTVIIQSFEKKLNKGGQFHV"
BASE COUNT         1067 a          754 c          546 g          969 t
ORIGIN      
        1 tagtagtaat ttgcagattg taacttattt aaaattgaac atcctccaac tttagatttt
       61 tagaaacgca tacactgctc ctatctagcg ataaatcgga gaagaaatat ttaacgaatg
      121 atagagaagt tctttaaata taacgtagaa cctgtatttg ataacaaatg tttctaacta
      181 aatactattc acaaacctct atcactctta tgtgggtata tgagttttat cctattatta
      241 ctcagataag gctaggagta aaaccgttcc attagcagtt tcactgaaaa cgtcccacaa
      301 tcacctaaaa cattataaac acaaaatgaa agagagaaag aactaaacag ataatactca
      361 atgaaaatca gactaggcga cgcagatgca gatagaactg aagttcatca aatcaagtca
      421 acaacgtctg attttgattt tcgaagagta taggcaataa gtcttcattc gcagttgttg
      481 agctgaaata atctgtctcc aaacgttttt actacactgg gatgttgttc aaactttgag
      541 aaatagtact gttttttata cagattcatt tttgcataca aaaaaacctg atcacacgat
      601 caggcttttc aggagattat gaaaaatatt taggattggg tatagtataa ccaatattac
      661 taaaagtcac atccgtctta agactgattt attgaatatg accaataaaa taagtagtgt
      721 cgtttaaaaa tagactgatt catacgggga ctgcactcat tactgttagc taccaaaact
      781 acaagcataa tttaagaacc tgtattcaca gttcaacgct tctattctaa ggctagtttt
      841 taagctactc atcattagtt tttttcaaaa tacaatcaat atcccccaaa aaaattacct
      901 tgattagcta aatctgtctc ttaatatgag tacttcgctt gtaaatacag gttctaagaa
      961 cagggcttta ccaaagcagg catggatata tttattttta ttaaactctc tatcttattt
     1021 tgcgcttctg acacaagctt cataggtctg ttccatcaac atggtaatag tcatcggacc
     1081 aacaccacct ggaactgggg taatataact tgccacttct gatacctcat cgtatttgac
     1141 atcaccgatt agcttgccat tttcatcacg attcatacca acatcaatca ccacggcacc
     1201 tggtttaaca aattccttgg tcacaaaatg accacgacca atggcaacaa caagaatatc
     1261 ggcctgacga gcaagctctg gcaagttttt ggtgcgagaa tgcgcaatcg ttacagtagc
     1321 attggcatcc aatagcagct gtgccattgg tttaccaaca atattagaac gaccaataac
     1381 taaagctgac ttcccttcca gctcaatctg atattccttg aacatttcca taattcctgc
     1441 tggggtagat ggaatcataa caggatgacc tgaccagaat ttccccatat tagtcggatg
     1501 aaaaccatcc acatccttat ctgggtcaat ggccaacaag accttctcct cgctgatgtg
     1561 ggctggtaag ggcaattgca ccaaaatacc atgccattca tcatcctgat tatagcgctc
     1621 aatcaaatcc aacaagtctg actcagaaat cgtatctgga acgcgaacaa cctcactctt
     1681 gaatccagca gctagagctg accgctcctt attccgtaca tagacctgac tagcaggatt
     1741 ttcgccaact aaaataacta caagacctgg taccaccccc ttttcttctt ttaatcgagc
     1801 agttttctcc gccaaagcag cctgcaattt tacacctagt gctttcccat caatgactgt
     1861 catacctaca ctccttttgt tttttctatt ataccctaaa attctccgac tgactatact
     1921 tgtacaaagt agactatagc atatagcaaa aaactccgaa caatgtcgga gtcgtactta
     1981 tttgttcatt tattacaaga ccttagacaa gaaatccttg gtccgttctt ccttggtatg
     2041 ttcaaagact tcttcaggcg ttccatcctc cacgatgata ccgccatcca taaagataac
     2101 cctgtcagct acctcacgcg caaatcccat ctcatgagtc acgataacca tggtcatccc
     2161 cgacttggcc aaatcctgca ttacagcaag aacttcacca accatttcag ggtctagtgc
     2221 agaggtcggt tcatcaaaaa gcaggacatc tgggtccatg gccagaccac gtgcaatagc
     2281 gatccgttgt tgctgaccac ctgaaaggct ttgtggatag gcatttgtct tatctggcaa
     2341 tcctaccttt tcaagtaatt ccttagcctt cttctcagcc tcatcctttg caattccctt
     2401 tgtcttaata ggtgataaag taatgttatc taataccgtc atatttggaa aaaggttgaa
     2461 ctgttgaaaa accattccca tcttttcacg catcttgaaa atatcatttg acttgtcagt
     2521 aatatcaaca ccttcaaatg taacagttcc cttggttggc acttctaaga ggttcattgt
     2581 acgtaagaaa gtcgatttcc ctgaccctga tggaccgata ataacgacaa cctgaccttg
     2641 ttgaatatct aaatcaattc cttttagaac ctcattcttt ccgaagtact tatgtaaatc
     2701 cttgatagaa ataatcgcat tagacatgga actgccctcc cttgttcaac tttttctcaa
     2761 acgactgaat aatcactgtt agcaccgttg tcataattag ataatagctc gctgcaaata
     2821 caagtggtgt taaaggaata taagaagtgg attgaactgt ctgagcaccg ttccataatt
     2881 ccatcacacc gatggttgac aaaagtgaac tatccttaac aatagtcacg aattcatttc
     2941 ccaaggctgg aagaatattc ttgatagcct gcggcatgat aacgtaacgc atcgcctgct
     3001 ttggtctaat accaagcgaa taagctgctt ccagctgtcc ttttggtaca gcattaatac
     3061 ctgcacgaac agtttcagat acataggcac ccgagttcat cgaaataaca atgatacctg
     3121 gaatcaagcg cgtcaaatca accgtcaaaa ttcctagctg aaaagttggt gctacaaaat
     3181 gtgtcatcgc aaaagcaatc ataatctgga caatcatcgg tgtcccacgg aaaatccaga
     3241 catagatatt ggcagtccaa gctaaaactt tatacttact acgctgagca aaggcaagta
     3301 acacacctaa aatactacca aaaaatactg agaaaa
//
LOCUS       DASFSW010000175         3325 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.176, whole genome shotgun sequence.
ACCESSION   DASFSW010000175 DASFSW010000000
VERSION     DASFSW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.176"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..805
                     /gene="dprA"
                     /locus_tag="U1099_001977"
     CDS             <1..805
                     /gene="dprA"
                     /locus_tag="U1099_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEM4868256.1"
                     /translation="LTNLNVNKVLAYQEKHGKKLTLRNVAVVSECKNPVLFMENYKNL
                     DTKACREEFNKYPSFSILDDIYPLALKQIYNPPVLLFYQGDLELLEKSKLAVVGTRNA
                     SKNGVQSVQKIIKELNNRFVIVSGLARGIDTAAHIASLKNGGQTIAVIGTGLDKIYPK
                     ENRELQTYIGKHHLVLTEYGPGTDPLKYHFPERNRIIAGLVEGLVVCEAKMRSGSLIT
                     CERALEEGREVYAIPGSIMDGKSDGCHHLIQEGAKCVTSGLDIISEYQF"
     gene            898..2991
                     /gene="topA"
                     /locus_tag="U1099_001978"
     CDS             898..2991
                     /gene="topA"
                     /locus_tag="U1099_001978"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEM4868257.1"
                     /translation="MATKTVSKKKTTTKKNLVIVESPAKAKTIEKYLGKNYKVVASVG
                     HIRDLKKSSMSIDFDNNYAPEYINIRGKGPLINSLKKEAKNAKQVFLASDPDREGEAI
                     SWHLAHILGLDANDKNRVVFNEITKDAVKNAFQEPRAINLDLVDAQQARRVLDRIVGY
                     SISPILWKKVKKSLSAGRVQSVALKLIIDRENEISNFKPEEYWTIDATFKKGSKKFQA
                     SFYGIDGKKTKIENNEQVKEILARLDGDDFLVEQVERKERKRNAPLPYTTSTMQMDAA
                     NKINFRTRKTMMVAQQLYEGVSLGTGGTQGLITYMRTDSTRISPVAQAQAADFITERF
                     GATYSKHGNKVKNASGAQDAHEAIRPSNVNLTPESIAKYLDKDQLRLYTLIWNRFVAS
                     QMAVAVFDTMSVRLGQNGIVFAANGSQVKFDGYMAVYNDADKNKMLPDMEQGDTVVRA
                     STNPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVKLAAKRFEPT
                     ELGEIVNKLIVEFFPDIVNVKFTADMEQKLDDVEIGKEQWQKVIDGFYQPFKVELSKA
                     EEQMEKIQIKDEPAGFDCDVCGHEMVIKLGRFGKFYACSNFPDCRNTKQITKEIGVTC
                     PVCQKGQVIERKSKRNRLFYGCDRYPECEFTSWDKPVGRSCPKCDHYLVEKKVRGGGK
                     QVVCPNGDYEEDKIK"
BASE COUNT         1105 a          603 c          713 g          904 t
ORIGIN      
        1 tttgaccaat cttaatgtga acaaagtttt ggcctatcaa gaaaaacacg gtaaaaaatt
       61 gaccttgcga aatgtcgctg tagtgtctga atgtaaaaat ccagttctat ttatggaaaa
      121 ttataagaat ctagatacaa aagcttgtcg cgaggaattt aataagtatc catcattttc
      181 aattttagat gacatctatc ctttggcgct caaacagatt tacaatccac ctgtactttt
      241 gttttatcaa ggggatttag aattgctaga aaaatcaaag ctggcagttg ttggtacacg
      301 aaatgccagt aaaaatggcg ttcagtctgt tcagaagatt atcaaagaac tcaacaatcg
      361 ctttgtcatt gtcagtggtt tggcaagagg aattgataca gctgcccata ttgctagcct
      421 gaaaaatggt ggacaaacca ttgccgttat tggaacaggt ttggataaga tatatcccaa
      481 agaaaataga gaattacaga cctacatcgg aaaacatcat ttggttctga ctgaatatgg
      541 accaggtaca gaccctctca agtaccattt cccagaacgc aatcgcataa tagctggttt
      601 ggtggaagga ttggtagtct gtgaagctaa gatgcggtct ggtagcctca ttacctgtga
      661 acgtgccttg gaagaaggaa gagaagtcta tgctattcca ggttccatta tggacggaaa
      721 atcagatggt tgtcatcatc tgattcaaga gggagcaaag tgcgttacat ccggcttgga
      781 catcatttca gagtatcaat tttaaaacaa gtcttcctat aggaaaataa atatcttgac
      841 actgactgaa aaatagtata ttctttgtga ggttaaaaat atttgtaagg tataagaatg
      901 gctacaaaaa cagttagtaa gaaaaaaaca acaaccaaga aaaatctcgt tattgtagag
      961 tcgcctgcta aggcaaaaac aatcgaaaaa tatcttggta aaaattataa ggtcgttgct
     1021 tcagtcgggc atattcgaga tttgaagaaa tctagtatgt ctattgattt tgataacaac
     1081 tatgcacctg agtacatcaa catacgtgga aaaggaccac tcatcaattc tttgaaaaaa
     1141 gaagcaaaaa atgctaagca agtctttctc gcgagtgacc cggaccgcga aggagaagca
     1201 atttcttggc atttggctca cattttggga ttggacgcaa acgataaaaa ccgtgttgtc
     1261 tttaatgaaa ttacaaaaga tgcagtaaaa aatgcctttc aggagccacg tgctattaac
     1321 ctagacttgg tagatgccca gcaagctcgt cgcgtcttag accgtattgt agggtactcc
     1381 atatcaccta ttttatggaa gaaagtcaaa aaaagtctgt cagcaggacg tgttcaatcg
     1441 gttgctctca aattaataat cgaccgtgaa aatgaaatca gcaatttcaa accagaagaa
     1501 tattggacaa ttgatgcaac cttcaaaaaa gggagcaaga aattccaggc ttcattctac
     1561 ggtatagatg gaaagaaaac taaaattgaa aacaatgagc aggttaaaga aattctcgcc
     1621 cgacttgatg gagatgattt cttagtagaa caggttgagc gtaaggaacg caaacgaaat
     1681 gcacctctac cttacacaac ttctactatg cagatggatg cggctaataa aatcaatttc
     1741 cgtactcgta aaaccatgat ggttgcccaa caactttatg agggtgtcag tcttggcaca
     1801 ggtggtaccc aaggtttgat tacctatatg cgtacggatt caacacgtat tagccctgtt
     1861 gcacaagcac aagctgctga ctttatcacg gagcgttttg gtgctaccta ttcaaagcat
     1921 ggtaataaag taaaaaatgc cagtggtgca caagatgccc acgaagccat tcgtccatca
     1981 aatgttaact taacacctga atcaattgca aaatatctgg acaaggacca gttgcgacta
     2041 tatacactga tttggaatcg ttttgtcgca agtcagatgg ctgtagcagt ttttgatacc
     2101 atgagtgttc gtttgggtca aaatggtatt gtctttgctg ctaatggtag ccaagtaaaa
     2161 ttcgatggtt atatggctgt ctataacgac gctgataaaa ataaaatgtt gccagatatg
     2221 gaacaaggtg acacggtggt gcgcgcgtca acaaatccag aacaacactt tactcagcca
     2281 ccagctcgct actctgaagc aaccttaatt aaaacattag aagagaatgg tgtaggtcgc
     2341 ccgtcaacct atgcaccaac gattgaaacc attcaaaaac gctattatgt taagcttgcg
     2401 gctaagcgat ttgaaccaac tgaactcgga gaaatcgtta ataagctgat tgttgaattt
     2461 ttcccagata ttgtcaatgt caaatttaca gccgatatgg agcaaaaatt ggatgatgta
     2521 gaaatcggga aagagcagtg gcagaaggtc attgacggtt tctatcagcc attcaaggta
     2581 gaattatcta aggcagaaga acagatggaa aaaatccaaa ttaaggatga accagctggc
     2641 tttgactgtg atgtttgtgg acatgaaatg gttattaaat taggtcgctt tggaaaattc
     2701 tatgcatgca gcaatttccc agactgtcgc aataccaagc aaatcactaa agaaatcggt
     2761 gtgacttgtc cagtttgcca aaaagggcaa gtgattgaac gcaagagtaa acgcaatcgt
     2821 cttttctatg gttgcgatcg ctacccagag tgtgaattta cttcttggga caagccagtc
     2881 gggcgttctt gtccgaaatg cgaccactat ctcgttgaga aaaaagttcg tggtggtggt
     2941 aaacaagttg tctgtccaaa tggtgactac gaagaagaca agattaaata agattaaact
     3001 gctattctca agggaatggc agttgttttt tagagtcatg tttagtttat cttagcttac
     3061 ttcgacaagc gaggaaactt cgtctcctta ttttcaactt taaacgaggc tggtcaaaaa
     3121 gtccaaagtc acttctcagt gcagtggttg attgggttta acaggccagt ggagtgaaac
     3181 tgttcagtga actgtttcag cctgagctta gaaatgaaaa aatcgaggta aaaaggttgg
     3241 agataagatt tgcgaagcaa atcacagcaa tttgtgtttc acattccaag tctgacctct
     3301 acaactaaaa cgaactaaac ggcaa
//
LOCUS       DASFSW010000176         3306 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.177, whole genome shotgun sequence.
ACCESSION   DASFSW010000176 DASFSW010000000
VERSION     DASFSW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.177"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            170..874
                     /locus_tag="U1099_001979"
     CDS             170..874
                     /locus_tag="U1099_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEM4868258.1"
                     /translation="MEKMRKVQFFLLIESMIFTMAAFDVVANEASRTLLLFSALLLVI
                     WYFLGRKVNSVLLVSSLSLLFSVFVLNPFFIIGIMLLVVYMLINFFSRYEKRNQYTHI
                     VFSDYSLQVQKEKNRWFGNHDHSQDRFGFEDINIVRLFGNDVVDLDKTVLVGRDNIVV
                     IRKTFGRTKIIVPIDVEVALSATSIYGRVSFLGESYWDLRNESIAVNSSDYQESHKRV
                     KVVTNNILGDVEVVRV"
     gene            871..1887
                     /locus_tag="U1099_001980"
     CDS             871..1887
                     /locus_tag="U1099_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM4868259.1"
                     /translation="MRKRTYLLLAWFACLIVFVVIASTLPLLNYSLLDVTLWISTEQL
                     VFTLILLVIVLTLFLAVMVQTVSLASTQVMRTKLQAILKNKSIRTDTESDQLLLQLSD
                     KVRNLTRQVQLVDNQDLVKKEEIVEGERKRIARDLHDTVSQELFATSMILSGLSSNLS
                     TLPQETLQEQLMLVKDMIESAQRDLRILLLHLRPSELESKTLVEGFELILREVSDKSN
                     IIVHFQHEVEELPKLIEEHLFRIAQEIISNTLRHAKAKHLDVYLLQKETELQLKMTDD
                     GIGFQQGADDELSYGLQNIRERVEDMAGTIKIRTAPNKGVAIDIRIPLLKGKEDEYDS
                     SDAG"
     gene            1859..2500
                     /locus_tag="U1099_001981"
     CDS             1859..2500
                     /locus_tag="U1099_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4868260.1"
                     /translation="MNTIRVMLVDDHEMVRLGLKSYLNLQPDVEVVAEASDGEEGLAK
                     ALEVKPDVVVMDLVMPKMTGVEATLALLKEWPQAQIVILTSYLDNEKIYPVLEAGARG
                     YMLKTSSADEILAAIRKVALGEYAIETEVEKKVEHHKRHPDLHDDLTAREREILTLLA
                     KGYDNQRIADESFISLKTVKTHVSNILSKLAVSDRTQAVVYAFQHGLVAQEDQ"
     gene            2550..>3306
                     /locus_tag="U1099_001982"
     CDS             2550..>3306
                     /locus_tag="U1099_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM4868261.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRVKNTGYIPESIQQVFNSLKE
                     KGILTGIASGRTPYGLVPEIKALKPDFFAMINGSYVEDAKGQVVYHQPMPQNIVESVL
                     NWAKEIGIEYGMLGSQKGTLSARTDRISQVIDLIYEGLEINPTFYKENDIYQLLTFEK
                     DGHEVELPEELQAELRSVRWDAISSDIVLKGSSKATGVAKVVEKLGLKPENVLVFGDG
                     LNDIELFDYAGISIAMGHSHPELQKHADYITKKV"
BASE COUNT         1016 a          584 c          729 g          977 t
ORIGIN      
        1 ttgatagtat agacaatatt tagttttata gccctttagt ttctctttga ttacttcatc
       61 aacttgcttt gaactaaaag taaactaaaa ctatatatca gtcagtccta tatttcctac
      121 tttggatgga gttcttttac ttattttttg ttacaatagt atagaaagga tggagaagat
      181 gcggaaagtt caattttttc tactaataga aagtatgatt tttacgatgg cagcctttga
      241 cgtggtagcc aatgaggcca gtcggaccct gctacttttc tctgccttat tactcgtcat
      301 ttggtacttc ctaggaagaa aagtcaacag tgttttactg gtcagctcct tatctctatt
      361 attttcggtc tttgttttaa atcccttctt cattattggg attatgctac tagtcgtgta
      421 catgctgatt aatttctttt cacgttatga aaagagaaac caatataccc acattgtttt
      481 tagtgattat tctctacagg ttcaaaaaga gaaaaataga tggtttggca accatgacca
      541 ctcacaagat aggtttggtt ttgaagatat taacattgta cgcttgtttg gcaatgatgt
      601 agttgatttg gataagactg tattagttgg cagagataat attgttgtaa ttcgtaaaac
      661 gtttggacgg acaaaaatta ttgttccaat tgatgttgag gtcgcacttt cagcaactag
      721 tatctatgga cgagtaagtt tcctgggtga atcatactgg gatttgcgga atgaaagtat
      781 agcggtcaat agttccgatt atcaagaatc acacaaacgt gtaaaagtcg ttactaataa
      841 tatactggga gatgtggagg tggttcgcgt atgagaaaac gtacctacct actcctcgcc
      901 tggtttgcct gtcttattgt ttttgtggtg attgcttcca ccctcccact tttaaattat
      961 tcattgttag atgttacgct ctggatttca acggagcagc tcgtgtttac tctcattcta
     1021 ctagttattg ttttgaccct ttttctagct gtaatggtac aaactgtcag cctagcctcc
     1081 actcaggtta tgcggacgaa gttacaagct atcttgaaga ataaaagtat tcgaacagat
     1141 accgaaagtg atcagctctt gctccagttg tctgataagg tcagaaacct cacgcgtcag
     1201 gtgcagctgg ttgacaatca agatttagtc aagaaagaag agattgttga gggagaacgg
     1261 aaacggattg ccagggattt gcatgatacg gttagtcaag agttatttgc aacgagcatg
     1321 attttgtcag gattatcgtc caatctatcc actcttccgc aggaaacttt gcaggaacag
     1381 ctgatgctcg tgaaagacat gattgagtct gcccaaagag acctacgcat cctattatta
     1441 catctacgcc caagtgaact tgaaagtaaa accttggttg aaggatttga gctgattttg
     1501 cgtgaagtta gcgacaaaag taatattatc gttcatttcc aacatgaagt agaggagtta
     1561 ccgaaactaa ttgaagaaca tctcttccgc attgcgcaag aaattatcag taataccttg
     1621 cgacatgcca aagccaagca tttggatgtt tacctcttgc agaaggaaac agaacttcag
     1681 ttgaaaatga cagacgatgg tatcggcttc caacaagggg cagatgatga attaagttac
     1741 ggtcttcaaa acatacgtga gcgggttgaa gacatggctg gaacgataaa aattcgcact
     1801 gctccaaata agggagtggc gattgatatt cgtatcccac tattgaaagg aaaagaagat
     1861 gaatacgatt cgagtgatgc tggttgacga ccatgagatg gttcgtctag gattgaaaag
     1921 ttacttaaat ttacagccag atgttgaagt agttgcagaa gctagtgatg gcgaggaagg
     1981 tttggctaaa gcattagagg ttaaacccga tgttgtagtg atggacttgg ttatgcctaa
     2041 gatgactggc gtagaagcaa ccctggcctt gctgaaggaa tggccgcaag cgcagattgt
     2101 catcctgacc tcctacctag ataatgagaa aatctatcct gtgctggaag caggagctcg
     2161 tggctatatg ttgaaaacgt caagtgctga cgaaatctta gccgctatcc gcaaggtagc
     2221 tcttggagaa tatgctattg agacagaagt agagaaaaaa gtagagcacc ataaacgcca
     2281 cccagattta catgatgact tgacagctcg tgaacgcgaa atcttgacgc tgttagccaa
     2341 gggatatgat aatcaacgaa ttgcagatga gtctttcatt tccctaaaaa ctgttaagac
     2401 ccatgtttcc aatatcttat ccaaactagc tgttagcgat cgtactcaag cagttgttta
     2461 tgctttccag catggtttgg tggcgcaaga ggaccaatag tggtataatg aaaattagtt
     2521 aattttcaac caaaggatag tggaattata tggatgcaaa actcagatat aaggcaaaaa
     2581 aaataaaaat agtctttttt gacattgacg acacgctacg tgtaaagaat acaggctaca
     2641 ttcctgaatc cattcagcag gttttcaact cactgaaaga aaagggaatt ttgacaggaa
     2701 ttgcgtcagg acgaacgcct tatggtttgg tgccagaaat caaggcctta aagccagact
     2761 ttttcgctat gattaacggc tcctacgtag aagatgccaa aggtcaggta gtgtaccatc
     2821 agccaatgcc ccaaaatata gtagaatctg tattaaattg ggctaaagaa attggaatcg
     2881 aatatggtat gcttggtagt caaaaaggga cactttctgc acgaactgac cgcattagtc
     2941 aagtaattga cttgatttat gaaggtttgg aaataaaccc aactttttat aaagaaaatg
     3001 acatctatca attgttaact ttcgagaaag atggtcacga agtagaactt ccagaagagt
     3061 tgcaagcgga attgcgtagt gttcgttggg atgctatttc atcggacatt gtgttaaaag
     3121 gctcttccaa agcaactggt gttgctaagg ttgttgaaaa actaggcctg aaacctgaaa
     3181 atgtcctagt gttcggagat ggactcaacg atattgagtt attcgattat gcaggaatta
     3241 gtatcgctat ggggcattcg catccagaac tgcaaaagca tgcagattat atcacaaaaa
     3301 aagtag
//
LOCUS       DASFSW010000177         3201 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.178, whole genome shotgun sequence.
ACCESSION   DASFSW010000177 DASFSW010000000
VERSION     DASFSW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.178"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            38..412
                     /locus_tag="U1099_001983"
     CDS             38..412
                     /locus_tag="U1099_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HEM4868262.1"
                     /translation="MAKTIFEEMGGKYERQGDYLIPCLTVPAEEEQPIGIWGQRHLDY
                     LKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQAQGITEQLKAENALEW
                     TGCLNNIRACAREIVEKEIIFA"
     gene            779..2698
                     /gene="tet(O)"
                     /locus_tag="U1099_001984"
     CDS             779..2698
                     /gene="tet(O)"
                     /locus_tag="U1099_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HEM4868263.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYKERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            2750..2923
                     /locus_tag="U1099_001985"
     CDS             2750..2923
                     /locus_tag="U1099_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEM4868264.1"
                     /translation="MMKCEWILCPVCGSKTRNKIRKDTVLENYPLYCPKCRQESLIKV
                     DNLKITVIKEPDA"
BASE COUNT         1054 a          536 c          729 g          882 t
ORIGIN      
        1 acattcaaaa taaaaaagaa tgtggaggta acagacaatg gcaaagacaa tttttgagga
       61 aatgggcggc aaatacgaaa ggcaaggcga ttatttaata ccgtgcttaa ctgtacccgc
      121 cgaagaagaa cagccgatag gcatctgggg acagcggcat ttggattatc tgaagcatca
      181 ctgcaaagtt acatacacca atcttcttac aagcggcaga cttaacgctt accttgccga
      241 cattgacaga caggcacagg aacgctttga aaggctcata gagggtatga aacaggcaca
      301 gggcataacg gaacagctaa aggcagaaaa cgccttagaa tggacaggat gcctcaataa
      361 cataagggct tgtgcgaggg agattgtgga aaaggaaatt atttttgcat aaacagatga
      421 ttagtggcag ggggaaatcc tgccgctttt tctgctttag tttgtcagct tgacaaataa
      481 agggttaagg aatataatta gattcagtat tatacaagga gttaataaat atgcggcaag
      541 gtattcttaa ataaactgtc aatttgatag tgggaacaaa aagtagcagt cccgtttcac
      601 ttttaatatg gggcttagtt ttttgtaccc agtttaagaa tacttttatc atgtaatttt
      661 atatgcccga aaacatataa gtgttttggg gctattggag ttatttaccc agtgatagga
      721 gtatttatca ctgggtattt ttatgccctt ttttgggtgt tgataggagg aaaatcacat
      781 gaaaataatt aacttaggca ttctggctca cgttgacgca ggaaagacaa cattaacgga
      841 gagtttattg tataccagtg gtgcaattgc agaaccaggg agcgtagata aaggcacaac
      901 aaggacagat acaatgaatt tggagcgtca aaggggaatc actatccaga cagcagtgac
      961 atcttttcag tgggaggatg taaaagtcaa cattatagat acgccaggcc atatggattt
     1021 tttggcggaa gtataccgtt ctttatccgt attagacgga gcagtattat tagtttctgc
     1081 aaaggatggc atacaggcac agacccgtat actgtttcat gcactacaga caatgaagat
     1141 tccgacaatt tttttcatca ataaaattga ccaagagggg attgatttgc caatggtata
     1201 tcaagaaatg aaagcaaagc tttcttcgga aattatagtg aagcaaaagg ttgggcagca
     1261 tccccatata aatgtaacgg acaatgacga tatggaacag tgggatgcgg taattatggg
     1321 aaacgatgaa ctattagaga aatatatgtc agggaaaccg tttaaaatgt cagaactgga
     1381 acaggaagaa aacaggagat tccaaaacgg aacgttattt cccgtttatc acggaagtgc
     1441 taaaaacaat ctggggattc ggcagcttat agaagtgatt gccagtaagt tttattcatc
     1501 aacgcctgaa ggtcaatctg aactatgcgg gcaggttttt aagattgaat attcagagaa
     1561 aaggcggcgt tttgtttatg tgcgtatata tagcggaaca ttgcatttga gggatgttat
     1621 taaaatatct gaaaaagaga aaataaaaat cacagagatg tgtgttccga caaacggtga
     1681 attatattca tccgatacag cctgctctgg tgatattgta attttaccaa atgatgtttt
     1741 gcagctaaac agtattttgg ggaacgaaat gctgttgccg cagagaaaat ttattgaaaa
     1801 tcctctccct atgctccaaa caacgattgc agtaaagaaa tctgaacagc gggaaatatt
     1861 gcttggggca cttacagaaa tttcagatgg cgaccctctt ttaaaatatt atgtggatac
     1921 tacaacgcat gagattatac tttctttttt ggggaatgtg cagatggaag tcatttgtgc
     1981 catccttgag gaaaaatacc atgtggaggc agaaataaaa gagcctactg ttatatataa
     2041 ggaaagaccg cttagaaaag cagaatatac catccacata gaagtcccgc caaatccttt
     2101 ctgggcttct gtcgggttgt ccatagagcc gctccctatt ggaagcggag tgcagtatga
     2161 aagcagagtt tcacttggat atttaaacca atcgttccaa aatgcggtta tggagggggt
     2221 tctttatggc tgcgagcagg ggctgtatgg atggaaagtg acagactgta aaatctgttt
     2281 tgaatatgga ttgtattata gtcctgtaag tacccccgca gactttcggc tgctttcccc
     2341 tatcgtattg gagcaggctt taaaaaaagc agggacagaa ctattagagc catatctcca
     2401 ctttgaaatt tatgcaccgc aggaatatct ctcacgggcg tatcatgatg ccccaaggta
     2461 ttgtgcagat attgtaagta ctcaggtaaa gaatgacgag gtcattctga aaggagaaat
     2521 ccctgccaga tgtattcaag aatacaggaa cgatttaact tatttcacaa atgggcaggg
     2581 agtctgcttg acagagttaa aaggatacca gccagctatt ggtaaattta tttgccaacc
     2641 ccgccgcccg aatagccgta tagataaggt tcggcatatg ttccacaagt tagcttaaca
     2701 gcttgcaaaa gtcatataaa atgagatttg aaaggattag agactaatta tgatgaaatg
     2761 cgaatggata ttgtgtcctg tttgtgggag caaaacccgt aataaaatta ggaaggacac
     2821 tgttttggag aattatcccc tttattgtcc aaaatgcaga caagaaagct tgattaaagt
     2881 tgacaacttg aagataactg tcatcaaaga gccagacgct taagacgcag agccgatgaa
     2941 attgtggaac aattcacgaa tcatcggctc tttttgtttc gtatttgaaa gaacaaactc
     3001 accaaataaa aaaaacgata ttcgggtggg ttattttgtt ataccctaaa ttaccctctg
     3061 aatttgtttt taaatttgga gggatttttt tatgttcttt tttcgggcgg ttattcatct
     3121 gctcatacga agtaaaattt atcaatacat agcatatcag agaatggcag gaaaccagtt
     3181 aaaaaaattt ctgccagtgc a
//
LOCUS       DASFSW010000178         3188 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.179, whole genome shotgun sequence.
ACCESSION   DASFSW010000178 DASFSW010000000
VERSION     DASFSW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.179"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..960)
                     /locus_tag="U1099_001986"
     CDS             complement(<1..960)
                     /locus_tag="U1099_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM4868265.1"
                     /translation="MATITKRGNSYRATVSLYKKGEYKRETKTFSNRKDAELWTLEME
                     LEKGRGKNIAERSTLFPDFYRNWVHTVKKTDVREATFINYKRTLVIVDDLFDGIQLKH
                     LDDLVMQKKIDQYAETHSKKTVKELVLKIRGSLKYAYARGLISNDFGHLLKSKGQEQP
                     KSITEFKKLRQYCLSHTENEFNVLVALALETVARRGELLGIKKEDIFEYGIKILRSIS
                     PTNDDTRLKTKYSKRDISINEDVYQAVTKLAQTKEGYIFDWNGFKQASQLQKLLKQLG
                     LTKTTFHGLRDTHASFLFSKDISLDYISRHLGHNSILTTQQYYL"
     gene            complement(1329..2084)
                     /locus_tag="U1099_001987"
     CDS             complement(1329..2084)
                     /locus_tag="U1099_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868266.1"
                     /translation="MALGNVEKDTEGWIELINQYLQYCIEIGLSPYTQATYKVALTKV
                     LGVSSTNFIATQPRTRANRMNNRVLHKDYRLSNKNNDYWHKVVTSTGLRKSELIHVTG
                     DALQRGRDGRWYLNLAGHKHHTKGRRDRWSPIMATSQEEEEWLVAIFQRAGEKKVFHV
                     PKDLILDDFDGKKVPTALKSHKYPTEYAERVYRSVAREISKIRNRKEVIHLRKELVGI
                     SLNRKACKIVIKTLGHNRPEEFPRSYAYILLKR"
     gene            2363..3130
                     /locus_tag="U1099_001988"
     CDS             2363..3130
                     /locus_tag="U1099_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM4868267.1"
                     /translation="MNKRERLEEITRLVNKKGTVRISEIVELLNVTDMTVRRDFIELE
                     EQGVLTKIHGGARSNKAFQYREYSHSEKHIQNKEAKQEIAERAAQLIEDGDTVFLGPG
                     TTVAFLAEAIKNSRLTVITNCMPVFTILSQKKTEEFQVFLLGGEYRQVTESFVGEITN
                     TSLEKMRFSKIFFSGNGLKGTDVMTSTLAEAYTQNIAIKHSLEKYLLLDASKIGKDDF
                     TSFCDLHDVTALVTDMNAKDENYQLLSKHIEIITTKL"
BASE COUNT          978 a          555 c          601 g         1054 t
ORIGIN      
        1 tagataatac tgttgtgttg ttaagataga gttatgacct agatgacgag aaatatagtc
       61 taggctaata tcttttgaaa agagaaatga ggcatgcgtg tcacgaagtc catgaaatgt
      121 cgtttttgtt aatcccaact gttttaacag cttttgaagt tgactagctt gcttgaatcc
      181 attccaatca aagatatagc cttcttttgt ttgtgcaagt tttgtcacag cttgataaac
      241 atcttcgttg atggaaatat cacgcttaga atatttggtt ttaagccggg tatcgtcatt
      301 agttggacta attgatcgaa gaattttgat accatattca aaaatatctt cttttttaat
      361 tcctaaaagt tctccgcgcc ttgctactgt ctcaagggcg agagcaacaa gaacattaaa
      421 ttcattctca gtatgactta ggcagtattg tctgagtttt ttaaattctg tgatagattt
      481 aggttgttct tgtccttttg atttcaaaag atgaccaaag tcattactaa tcaaaccacg
      541 agcatacgca tattttaatg aaccacggat tttaagaaca agttctttta ctgttttttt
      601 agagtgggtt tcagcgtatt ggtcgatttt cttttgcatg acgagatcgt caaggtgttt
      661 aagttgaata ccatcaaaaa ggtcatcaac tattacaaga gttcgtttat agttaataaa
      721 ggtggcttcg cgaacgtcag ttttcttgac cgtatggacc caatttctat aaaaatcagg
      781 aaataaggta gatcgttcgg caatgttctt gccacgacct ttttcaagtt ccatttctaa
      841 tgtccaaagc tcagcatctt ttctgttgct aaaggtttta gtttctcgtt tatattctcc
      901 tttcttataa agtgatacag ttgcacggta agagttacca cgttttgtta ttgttgccat
      961 tttatcagcc tttctataaa aaatgattga taaaatagac tagtcacata tagggatata
     1021 tgaaaaatct aattttatca atcattatcc gtcaatcgca gtggctttca ataaatttaa
     1081 ccagttttga cttcaaatag cgctcttgtt ttcccaccat aaagcattga aagtctgggt
     1141 gacttctgat gtacttatca aatgatttag gatctattcc aagtagttcc gctgcttgtt
     1201 gtctatttaa cagaagagga gaattatttt gaggtaacat atagaattcc tttcattttc
     1261 tagatacaag tattatgcca catgcgtaat gaattgaggc tagatttttg aaaattgttc
     1321 aatgttacct aacgtttcaa taaaatataa gcgtaggaac gaggaaattc ctctggtcta
     1381 ttgtgtccca gagtctttat cacaatttta caagcttttc tgttgagtga gatgccaact
     1441 aactctttac gcagatggat aacttcttta cgatttctaa tcttggagat ttcgcgtgct
     1501 acactgcgat aaacacgctc tgcatactct gtaggatatt tgtgtgactt taatgctgtt
     1561 ggaactttct ttccatcaaa atcgtcgagg attaaatctt tgggaacatg aaagactttc
     1621 ttttctcctg ctcgttggaa aatagcgact aaccactctt cctcctcttg actagtggcc
     1681 atgattggag accagcgatc tctgcgtcct tttgtgtgat gcttatggcc ggctaaattt
     1741 aaataccaac ggccatcacg gccgcgttgc agagcatcgc cagtgacgtg gattaattca
     1801 cttttgcgta agcctgtcga ggtaactact ttatgccagt agtcgttatt tttgttggat
     1861 aaacgatagt ctttatgtaa tacacgattg ttcatacgat tcgcacgagt acgtggttgt
     1921 gtagcgatga aattagttga ggaaactccg agaactttgg tcaatgctac tttgtaggta
     1981 gcttgtgtat atggacttaa accaatctct atacaatatt gtaagtattg attgataagt
     2041 tctatccaac cttccgtatc tttttcaaca ttacctaatg ccattaactg ttctggcgaa
     2101 atgttaattg aaacccattt tgcaaaacaa tgccatgaac ctgaatagtt atggagtgta
     2161 cgatgagtgt ggattaactc aatgctttct tttttccttg cagtcggttc taacgaaccg
     2221 atgttttgcg aaccgcgcaa aacctgcttg cgaagattgc tcgcataata aagttgtaat
     2281 tatcatttaa aataatgttc aaaattacaa cacattcttg tttatttttg atataatgta
     2341 aaaaaacaac aaaggattaa acatgaataa acgagaacga ctagaagaaa ttacacgatt
     2401 ggtaaacaaa aaaggcacag tccgaatttc tgagattgtt gaattgctaa atgttactga
     2461 tatgactgtt cgacgtgatt ttattgaact ggaagagcaa ggtgtcttaa caaaaattca
     2521 cggcggtgct agaagtaata aggcttttca atatcgggaa tactcccatt ctgagaaaca
     2581 cattcagaat aaggaagcca agcaagagat agcggaaagg gctgcacaac tgatagaaga
     2641 tggagacacg gtatttttag gtccaggaac aacagtggcc tttttggctg aggccattaa
     2701 aaattctcgc ctaacagtca tcacaaactg tatgcctgtt tttaccatcc tatctcaaaa
     2761 gaagacagag gagtttcaag tctttcttct ggggggagaa tatcgtcaag tgacagaatc
     2821 ttttgtagga gaaattacca atacaagttt agaaaaaatg cgattcagca agattttctt
     2881 ttcaggtaac ggacttaagg gaactgatgt catgacctct accctagcgg aagcctatac
     2941 gcaaaatatt gccattaaac attctttaga aaaatatcta ctgctagatg cctcgaaaat
     3001 tggaaaagat gattttactt ccttttgtga tttgcatgat gtaactgctc tagtgaccga
     3061 tatgaatgca aaggatgaaa attaccaatt attaagtaag cacatcgaaa ttatcactac
     3121 aaaattataa agttatcaaa accaacagaa aagtgttggt tttttgtttg tacacgaaaa
     3181 aacaaaca
//
LOCUS       DASFSW010000179         3179 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.180, whole genome shotgun sequence.
ACCESSION   DASFSW010000179 DASFSW010000000
VERSION     DASFSW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.180"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1289
                     /locus_tag="U1099_001989"
     CDS             <1..1289
                     /locus_tag="U1099_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEM4868268.1"
                     /translation="LRLAIPFLLISIFVMPVTKVPDEATHAFMSWNILFDSPTSRDTE
                     AMNELRAADFAYSEPEIHAVDSTEYSSLFTKIRDFSKDKLSIDFSLKSLMSTPQLIGL
                     VVGKLIYPSYGMMVTIGRLFNTIVYIIGIYYLIKKIKYGKMTLFFVSLLPMMIQQAGS
                     LSYDVVNYLAVVAFFVFYVNLLVDKVLTTRKFINLILLALLLYLTKANNILLLALLFF
                     VDFEFEGILSKFNLILKWIQKRRLPILILGLGLVLLVSYLFLHNRGGVVHFVKVMINS
                     LFINNRNDHLNGILTVGIFGYFGWFVTQLPLWLIFIDIFIFTLLLFNDGNLKISKTEG
                     LVSLFVLPVQVAIIVAGMYFAWTPTAIGPNAMISQGAQGRYFTPFLVYLFPACLMLKE
                     QVSVSVKQKYLIRLLTGTVVINFIMYLFLIVDYYWL"
     gene            1312..2301
                     /locus_tag="U1099_001990"
     CDS             1312..2301
                     /locus_tag="U1099_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM4868269.1"
                     /translation="MNILFTLNDAFVPQVAACMGSIMRTLNEDDTCHFYLFSDGISQQ
                     NKEKLHQFVTDGGNKLTVVELENLESYFDFEVDTNGWASVVLARLLVDKLLPKEVDRI
                     IYLDGDTLVLENIRELWEVDLEGKVLGMCPEPTASSERREGLNLGTYTYHNAGVLLID
                     LKRWRSKSIGTIIFDYYKEKNGELFANDQDALNGALKEEIKTLSITYNYFNIFDVYPY
                     RTLEKLSRPSTFISKEEFVKIRKQPRIVHFLGEERPWRIGNKHRFREDYVSALNQTPW
                     RGTQFESGWQFYFFCFNLFNMVMKPFPMLRYKIITVLIPVFMKYRKIRLQKGV"
     gene            2304..>3179
                     /locus_tag="U1099_001991"
     CDS             2304..>3179
                     /locus_tag="U1099_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4868270.1"
                     /translation="MKFSVIIPAYNVADYLEECVYSVLNQTYEEFEILLVDDGSTDGI
                     TSNICDKLAAKDDRVKVFHQTNGGQSIARNTGIKNASGDYILFLDGDDFWTDVHFLDE
                     INEELHSHEEVVDAVIYPFSYWYGNADIRVRDFPQKLPRHEIITDSVLLVETGALIAP
                     VWNKCVRRELFADSLMFPDGLMYEDGIWCADLLKIITCCCVIENSNYMYRQNRDGSFT
                     NKITQKKVYHAFRGIEENLKNFKQLSNEKQEALLIYLSNSYISILPFVFPYLNNSDIK
                     MFVKKFRYLLKYSQKV"
BASE COUNT         1015 a          425 c          619 g         1120 t
ORIGIN      
        1 ttttaaggtt agcaatcccc tttttgttga tttcaatatt tgttatgcct gtgacaaagg
       61 ttcctgatga agcaacacat gcttttatgt cgtggaatat attatttgat tctccgacaa
      121 gtagagatac tgaagcaatg aatgaattac gggcagcaga ttttgcgtat tcagaaccgg
      181 agattcatgc agttgattct acagaatatt caagcctttt taccaaaatt cgtgattttt
      241 caaaggacaa attatctatc gatttttctc tgaaatcgtt aatgagtaca cctcagctaa
      301 ttggtctagt tgtaggtaaa ttgatttatc cgtcatatgg tatgatggtc actatagggc
      361 gcctttttaa tacaatagtc tatattatag gtatctacta tttgataaaa aaaataaagt
      421 atgggaagat gaccctgttt tttgtctcac ttttacctat gatgattcag caggctggct
      481 cactgtctta tgatgtggtg aactatctgg ctgttgttgc attttttgtt ttttatgtta
      541 acttattggt agacaaggta ctgaccacca ggaaatttat taacttaata ttactagcct
      601 tgctactata tttaacaaaa gcaaataata ttttactttt agcactattg ttttttgttg
      661 attttgaatt tgaaggcatc ttatctaagt ttaatttgat tttaaaatgg attcaaaaga
      721 gaagattacc aattcttatt ttgggattag gactagtttt acttgttagc tatctattcc
      781 tacataatag aggtggtgtc gtgcactttg tgaaagtgat gataaacagt ttatttatca
      841 ataacagaaa cgatcatctt aatggtattt taacagttgg tatttttggt tattttggct
      901 ggtttgtcac tcaactacct ctctggttaa ttttcataga tattttcatt tttacgctat
      961 tattatttaa tgatggaaat ttaaagataa gtaaaacaga aggtcttgtg tcgttatttg
     1021 tactccctgt tcaggtagct atcattgtag cgggcatgta ttttgcatgg acaccgacag
     1081 ctattggacc aaatgcaatg atttctcaag gggcacaagg aagatacttt actccgtttt
     1141 tagtctatct atttccggca tgtttaatgt taaaagagca ggtaagtgta tcggtaaaac
     1201 agaagtattt gatacggttg ctcactggca cagtagtcat taactttatc atgtatctat
     1261 ttttgatagt ggattattat tggttgtaag aagatagtgg aagagggaaa cgtgaatata
     1321 ttatttacat tgaatgatgc ttttgtcccg caagttgcag cttgtatggg ctcaatcatg
     1381 cgaacattaa acgaagacga tacatgccat ttttatcttt tctcagatgg tataagtcaa
     1441 cagaataaag aaaaacttca tcaatttgtt accgacggag gaaataaatt gacagtcgtt
     1501 gaattggaaa acttggaatc atattttgat tttgaagttg acacaaatgg ttgggcttcg
     1561 gttgtacttg caagattact tgtagataaa ttactaccca aagaagttga tagaatcatt
     1621 tatttagatg gagatacttt agttttagag aatattcggg agctatggga agtagatttg
     1681 gaggggaaag tccttggaat gtgcccagaa ccgacagctt cgtctgagag aagagaaggc
     1741 ttaaatttgg gtacgtatac atatcataat gcgggagttc ttctaatcga tttgaaacgg
     1801 tggcgttcaa aaagtatagg aacaattatt tttgattatt ataaagaaaa aaatggagag
     1861 ctttttgcta atgatcaaga tgcactgaat ggagcattga aagaagagat aaaaacactc
     1921 tcaatcactt ataattattt taatattttt gatgtttatc catatcgtac gttggaaaaa
     1981 ttaagtaggc cttcaacgtt tatttcaaaa gaagaatttg taaaaattcg taagcaacca
     2041 cgtattgttc attttttagg tgaagaacgt ccatggagaa tcggaaataa acatcgattt
     2101 agagaggact atgtttctgc attaaatcag acgccatgga gaggtactca atttgagagt
     2161 ggttggcagt tttacttttt ctgttttaat ctttttaaca tggttatgaa gccttttcca
     2221 atgttgcgtt ataaaattat tacagtctta attcctgtat tcatgaaata taggaaaatt
     2281 agattacaaa aaggagtcta ggtgtgaagt tttccgttat tatcccagcg tacaatgttg
     2341 ctgattatct ggaagaatgt gtgtatagtg tactgaatca gacttatgaa gaatttgaaa
     2401 tattgttggt agatgacggt tctacagatg gtataacctc caatatatgc gataaattag
     2461 cagctaaaga tgatagggtg aaggtatttc atcaaactaa tggtgggcaa tcaattgcga
     2521 gaaatacagg aataaaaaat gctagtggtg attatatttt atttctagat ggtgatgatt
     2581 tttggactga tgtgcatttt ttagatgaaa tcaatgaaga actacactct cacgaggaag
     2641 tggtagatgc agtaatttat ccattttcat actggtatgg taatgctgat ataagagttc
     2701 gtgatttccc tcaaaaatta cctagacatg aaattattac tgattctgtt ctacttgtag
     2761 aaacaggcgc attgattgct cctgtttgga acaagtgtgt ccgcagagaa ctatttgcag
     2821 attctttgat gtttccagat gggttaatgt acgaagatgg tatttggtgt gcagacctat
     2881 taaaaataat aacttgttgt tgtgtcattg aaaattctaa ttatatgtat agacaaaata
     2941 gagatggaag ttttactaac aaaattacac aaaaaaaagt ctatcatgcc tttagaggaa
     3001 ttgaagaaaa tttaaagaat tttaaacaac tatcgaatga gaaacaagaa gcacttttga
     3061 tttatttatc aaattcttat atttccatct tgccatttgt atttccatat ctgaataatt
     3121 ctgatataaa aatgtttgtg aaaaaattta gatatttgct aaaatattca cagaaggta
//
LOCUS       DASFSW010000180         3152 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.181, whole genome shotgun sequence.
ACCESSION   DASFSW010000180 DASFSW010000000
VERSION     DASFSW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.181"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            94..516
                     /gene="lacA"
                     /locus_tag="U1099_001992"
     CDS             94..516
                     /gene="lacA"
                     /locus_tag="U1099_001992"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEM4868271.1"
                     /translation="MTIIIGADPAGSKLKDVIKDYLLEKSYDFKDVSDGSDFVDTTLA
                     VVAEVNQQEGNLGIVIDAYGVGPFMTATKVKGMIAAEVSDERSAYMTRGHNNSRIITI
                     GSEIVGEGIAKNIVKGFVEGEYDGGRHQIRVDMLNKMC"
     gene            543..1058
                     /gene="lacB"
                     /locus_tag="U1099_001993"
     CDS             543..1058
                     /gene="lacB"
                     /locus_tag="U1099_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEM4868272.1"
                     /translation="MKIAIGCDHIVTYEKIAVVDYLKNQGYEVLDFGTYDNVRTHYPI
                     FGKKVGEAVASGQADLGICICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGKITGELLMCDIIEAFIKAEYKPTEANKRLIEKIAAVETLNQEQANPEFFTEF
                     LEKWDRGEYQD"
     gene            1068..2000
                     /locus_tag="U1099_001994"
     CDS             1068..2000
                     /locus_tag="U1099_001994"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEM4868273.1"
                     /translation="MILTITLNPSVDIAYQLDTFHLDTVNRVEKVQKTAGGKGLNVTR
                     VLKQIGEDVVATGFIGGEIGSYVKKQLTRNDIKNSFVEIGSETRNCIAVLHDGKQTEI
                     LEQGPTIQEHEALNFIEHLEIILNNVDVVVISGSLPKGLASNYYVEIVELCKKCGVAV
                     VLDCSGEALKNVLESQQKPTVIKPNTEELSQLIGKEVTDDIQELKSVLSGQLFQGIDW
                     IVVSLGAQGAFAKYKDKFYRVRIPKIKVVNPVGSGDSTVAGIAAGLVHALPEAELLKN
                     ANVLGMLNAQEEQTGYVNLVHSESLYSQIEVEEV"
     gene            2002..2979
                     /gene="lacD"
                     /locus_tag="U1099_001995"
                     /pseudo
     CDS             2002..2979
                     /gene="lacD"
                     /locus_tag="U1099_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775145.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
BASE COUNT         1017 a          487 c          733 g          915 t
ORIGIN      
        1 ataaaaagca aaaacgcttt ttctaagttt tgcaaaacaa agattttctg ccaaatatgg
       61 ctacaacttt ctatttttgt taggagatag gttatgacaa tcattatcgg tgctgatcca
      121 gcgggttcaa aactaaaaga cgttattaaa gattatttgc tagaaaaatc atatgacttt
      181 aaagatgtca gtgatggaag tgattttgtt gatacaactt tggcagtagt tgcagaagtt
      241 aatcaacagg aaggaaatct tggcattgtg attgatgctt atggtgttgg tccatttatg
      301 accgcaacta aagtcaaagg catgattgct gcagaagttt cagacgaacg ttctgcctat
      361 atgacaagag gacacaacaa ttcgcgtatt attacaatcg gtagtgagat tgtaggagaa
      421 ggaattgcta aaaatatcgt taaaggattt gttgaaggtg agtatgatgg tggccgtcac
      481 caaattcgtg tcgatatgct aaataaaatg tgctaattgt tgtaaacaaa ggagaataag
      541 agatgaaaat tgcaatcggt tgtgaccaca ttgtaaccta tgaaaaaatc gctgtggtag
      601 attatttgaa aaatcaagga tatgaagtgt tggactttgg gacttatgac aatgttcgta
      661 ctcattaccc aatctttggt aaaaaagttg gcgaagctgt tgcaagcgga caagcagatt
      721 tgggtatttg tatctgtgga acaggtgttg gaatcaacaa tgctgtaaac aaggttcctg
      781 gtgttcgatc tgccctggtt cgtgatatga cctccgcgct ttacgcgaag gaagagttaa
      841 acgccaacgt cattggtttt ggtgggaaaa ttacaggtga gttgctcatg tgcgacatca
      901 ttgaagcctt cattaaagca gaatacaaac caactgaagc aaacaaacgt ttgattgaaa
      961 agattgctgc agttgaaacg ctcaatcaag agcaggctaa tccagagttc tttactgaat
     1021 ttcttgaaaa gtgggatcgt ggagaatacc aggactagga ggctcacatg attttaacaa
     1081 tcactttaaa tccgtcagtt gatattgcct atcaattgga tacctttcat ttggatacgg
     1141 tcaatcgagt agaaaaagtt caaaaaacag ctggtggtaa ggggctcaat gttacacgag
     1201 ttttgaaaca aatcggtgaa gatgttgtag caacaggctt tattggcggt gagattggaa
     1261 gttatgtaaa gaaacaactc actcgaaatg atattaaaaa ttcctttgtg gaaattggga
     1321 gcgaaacacg taactgtatt gcggttcttc atgatggcaa acagacggag attttagaac
     1381 aaggaccaac tattcaagaa catgaagcct tgaattttat cgaacacctt gaaattattc
     1441 tgaacaatgt agatgttgtt gttatttcag gaagtttacc taagggactt gctagcaatt
     1501 attatgttga gattgtagaa ctttgtaaaa aatgcggtgt agctgttgtt ctggattgtt
     1561 ctggtgaggc gctgaaaaac gttttagaaa gccagcagaa gccgactgtg attaagccga
     1621 atacagagga gttatcacaa ctgattggta aagaagttac tgatgatatt caagagttga
     1681 aatcagtttt gtctggtcaa cttttccaag gaattgattg gatagtcgtc tcattaggag
     1741 cacagggggc ttttgccaag tacaaagaca agttctatcg tgtcagaatt ccaaaaatca
     1801 aagtcgtcaa tccagtagga tcaggagatt caactgttgc aggtattgca gcaggtttgg
     1861 tacatgcttt acctgaagca gaattattaa aaaatgctaa tgttttgggc atgttaaatg
     1921 cccaggaaga acagacaggt tatgttaatt tagtacattc agaatcattg tattctcaaa
     1981 tcgaagtcga ggaggtttaa gatggtagta acagcagata aaagaaaata tatggaaaaa
     2041 gtgagaaatg aagcaggtat tatttcagct ttagcttttg accaacgtgg agccctgaaa
     2101 aaaatgatgg caaaacatca gacagaagaa cctactgtgg aacagatgga agaactaaag
     2161 agactagttt cggaagaatt aacacagttt tcttcctcaa ttctgttaga tcctgaattt
     2221 ggtttgccag catcacgcgc tcgtaacgag cagtgcggtt tgctcctagc ttatgaaaaa
     2281 actggctatg ataccagctc tactagtcgt ttaccagatt gcctagtaga gtggtctgta
     2341 aaacgactaa aagaagaagg ggcagatgcg attaagtttc tactctacta tgacgttgat
     2401 ggtgatccat acgtcaatct tcaaaaacag gcctatattg aacgcttggg ttcagaatgt
     2461 caggctgaag gtttaccatt cttcttggaa attttgagct atgatgaaac gattgaagac
     2521 aatgctagtg tggaatttgc aaaagtcaaa cctcgataag taaatgaggc catgagagta
     2581 ttttcggatg aacgctttgg agtggatgtt ctcaaggtag aagtacctgt gaacatgaat
     2641 tttgtagaag gctttggaac aggggaagtt gtctatacaa aagaagaagc agccgaatat
     2701 ttccgtcagc aagaagcgtc aactaactta ccatatattt atctaagtgc aggtgtttcc
     2761 gctaaacttt tccaagatac actagtgttt gcacatgaag caggtgctaa atttaacggt
     2821 gttctctgcg gtcgtgcgac gtgggcgggt gttgtgccag tctatattga acagggtgaa
     2881 gaagctgcgc gtgaatggtt acgtaccgtt ggtcgtgaaa atatcgaggg acttgatgca
     2941 gtcttgtctc aaacagcaac ttcttggcta gaaaaataat tgaaaaaata accattttct
     3001 caaaagaaag ggtttacatt ttctgagaat atgctataat aatctcatca gcaagaatag
     3061 agtggattgt aactaattcg attaggcaag actacaaatg gatttgagag taggatattc
     3121 tcggtttgtt tgtagtcttt ttttgcttaa aa
//
LOCUS       DASFSW010000181         3142 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.182, whole genome shotgun sequence.
ACCESSION   DASFSW010000181 DASFSW010000000
VERSION     DASFSW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.182"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(42..401)
                     /gene="rsfS"
                     /locus_tag="U1099_001996"
     CDS             complement(42..401)
                     /gene="rsfS"
                     /locus_tag="U1099_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEM4868274.1"
                     /translation="MKELDLVKVVVQAADDKRAEDLVVIDVQGVTSLTDYFVIASSMN
                     SRQLEAIAENIREKVAEVGIKSSRVEGDSNGGWVLLDLGGLVVHIFSEEMRDLYNLEK
                     LWHEGSFLEVADFLEEE"
     gene            complement(403..912)
                     /locus_tag="U1099_001997"
     CDS             complement(403..912)
                     /locus_tag="U1099_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM4868275.1"
                     /translation="MKTALIVIDIQSMPVEEQPYAIEERLALWQDSLAQARQAGLEVI
                     HVRHHDQELVKGTADWEIHSIVAPLASEKIFDKTFNSAFKETRLHAYLQEKEIERLII
                     MGMATNFCIDTTIKVAFELGYKVAVIQDGTTTGYSGKLDAEDLIDHYQNIWSWNFAQV
                     DKLENIIRG"
     gene            complement(922..1364)
                     /locus_tag="U1099_001998"
                     /pseudo
     CDS             complement(922..1364)
                     /locus_tag="U1099_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735474.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1367..1954)
                     /gene="yqeK"
                     /locus_tag="U1099_001999"
     CDS             complement(1367..1954)
                     /gene="yqeK"
                     /locus_tag="U1099_001999"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEM4868276.1"
                     /translation="MIAADLTFDRQALLEKIRVAMKPARFQHVLGVEEAALALADRYG
                     CDPKKASLAALLHDYAKEVEDQVFLDLIAKYDLDKALLNWDNNIWHGVVGAYKIAEDF
                     GLEDQEIFQAIQRHTIGAGQMTLLDKVLYVADYIEPNRDFPGVDEARRIAKESLDKAV
                     AYETAQTISYLAKKGIPIYPQTLETYNGYVVWLKE"
     gene            complement(1951..2583)
                     /locus_tag="U1099_002000"
     CDS             complement(1951..2583)
                     /locus_tag="U1099_002000"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEM4868277.1"
                     /translation="MAIELLTPFTKVELEVEKKETNRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLKLDEVLLMPEFIPPHVDKKETIDEYHRYSMLKMAIAGIEGLGIETIELERR
                     GVSYTYDTMKLLKEKNPNTDYYFIIGADMVDYLPKWHRIDELVQLVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRSFIKQGRVPNFMVPHEVLDYIEEKGLYQ"
     gene            complement(2596..2907)
                     /gene="yhbY"
                     /locus_tag="U1099_002001"
     CDS             complement(2596..2907)
                     /gene="yhbY"
                     /locus_tag="U1099_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEM4868278.1"
                     /translation="MMLTSKQRAFLNSQAHSLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKQVKE
                     I"
BASE COUNT          839 a          794 c          598 g          911 t
ORIGIN      
        1 ttttatggtg accagctgaa acaattcccc aaactgtttc actattcctc ttctaaaaag
       61 tctgcgactt ccaggaagct gccttcatgc cataattttt caaggttgta gaggtcacgc
      121 atttcttctg agaagatatg aacgactagg cctccaaggt ccaaaaggac ccaaccaccg
      181 ttactatcgc cttctacacg gctactcttg attccaactt ccgctacctt ttcacggatg
      241 ttttccgcaa tagcctccaa ctggcgactg ttcatggaac tggcaatcac aaagtagtct
      301 gtcaggctgg tcacgccttg aacatcaatt acaacaaggt cctcagctcg cttgtcatca
      361 gcagcctgaa ccacaacttt tactaaatct agttctttca tcttatcctc taattatatt
      421 ttctaattta tccacctggg caaaattcca tgaccagatg ttttggtagt ggtcaatcaa
      481 gtcttcggca tccaatttgc ctgaataacc tgtcgtcgtt ccgtcctgga tcacagctac
      541 cttgtagcct agttcaaagg ctactttaat cgtcgtatca atgcaaaaat tggtcgccat
      601 gcccatgatg atgagtcgct caatttcctt ttcctgcaaa taagcatgaa gcctcgtttc
      661 cttgaaggca ctgttaaagg tcttatcgaa aatcttctca ctagctaagg gtgcaacaat
      721 tgagtgaatc tcccagtcag cagtcccctt gaccaattcc tgatcatgat gtcggacatg
      781 gatgacttct agccccgctt gacgggcttg agcaagacta tcttgccaaa gagccagtcg
      841 ttcttcaatt gcatagggtt gctcctccac aggcatactc tgaatgtcaa tgactatcaa
      901 ggctgttttc atatccactc cctaaggcgt ttccaaacgg aagaccaatt ccctttcttc
      961 ttcactatcc aagacaaaat catttttctc caagagatga gccatcttct gattgccctt
     1021 aacataagct gcaatcaaat ggtcgcagac aggatgtgat tgagccattg ctattacaac
     1081 ctccaaagca gcttgaccat agccccttcc ttgaaactct tggcaatcat aaaccgccag
     1141 agcatatact gctggatgtc ctcatcaatg tcaatcaagg caaaaccgac cacctgttga
     1201 cccgcccaaa cagcgtaagg gaagacatcc ccgttttctc tgtaaagcca agcgtcagcg
     1261 agtgaacgca catttggagc cacgaagccc ttgtcctcct cggctactgt caagtccaag
     1321 actgcttgat aggagctttc atccacaggt tttaatgcaa tcatccctac tccttcaacc
     1381 aaacaacata gccattatag gtttccaagg tttgtggata aatgggaatt cccttcttgg
     1441 ctaagtaaga aatggtttgg gcagtttcat aggctactgc cttgtccaag gactcttttg
     1501 cgatacgacg cgcctcgtcc accccaggaa aatcccgatt gggttcgata tagtccgcca
     1561 catagagaac cttgtccagc aaggtcatct gcccagcacc aatggtgtgt cgttgaatgg
     1621 cttggaagat ttcttggtct tccaaaccaa aatcttctgc gattttatag gccccaacca
     1681 caccgtgcca gatgttatta tcccagttga gcagggcctt gtccaaatca tacttggcaa
     1741 tcaagtccaa aaaaacttgg tcttccactt ccttggcata gtcatgcaag agtgctgcca
     1801 gactggcttt tttcggatca cagccgtatc gatctgccaa ggcaagcgcc gcctcttcca
     1861 cacccaagac atgttggaag cgcgctggct tcatggctac acggattttt tccaaaagag
     1921 cttgacggtc aaaggtcaaa tctgctgcaa tcattggtaa agtccttttt cttcgatata
     1981 gtccagaact tcatgaggca ccataaaatt cggcacccgt ccttgcttga taaagctccg
     2041 taccatgctg gaggaaatgt ccatcaaggg cacatctacc cagataaccg gataggacgt
     2101 ccctgccttg taacgaggac gctggacacc aacaaactgc accaactgga ccaattcatc
     2161 aatcctgtgc cattttggaa ggtagtctac catatctgca ccgatgatga aataataatc
     2221 cgtattagga tttttttcct tcagcaattt cattgtgtcg taggtatagc tgacaccacg
     2281 gcgttctagt tcaatggttt caatccccaa gccctcaata cctgcaatag ccatcttgag
     2341 catggaatag cgatggtatt cgtcaatggt ttctttttta tccacatgag gcggaatgaa
     2401 ttctggcatg agcaagactt catccagctt caactgttga cggacctggt ctgccacaat
     2461 caaatgagca ttatggacag ggttgaaatt ccctcccaga atccccactt gtttgcgatt
     2521 ggtttctttc ttttcaacct ctaattcaac cttggtaaag ggtgttaaga gttcaatagc
     2581 cataggctct ccttcttaga tttctttgac ctgtttagaa attttacggt tctctttctt
     2641 gctagattgt ttgaataaaa tcaagatgcg accgattttt tggaccgtat ccacaccgat
     2701 ttcttcttcc aagatttctg ctacttcgtg aatgttttca tccgtatttt gtagcaaggt
     2761 caccttaatc aattcacgcg catccaacgc ctgacgaaca ctggttttaa tctggtcatt
     2821 tagaccattt ttcccaatct gaataatggg tttgagacta tgtgcctgac tgttcaaaaa
     2881 ggcacgttgt tttgaagtta gcataattta ttatacaaag ggcgttgtaa cagactggga
     2941 gactgttaca atcgggaaat aaagaaaaca aagtttttcc ttattgcgcc gatatactta
     3001 gcccgtgttc aaatcagaag acttgaactt cctttctctt taattgtaaa aactgaaatc
     3061 aaatacctct tatactcaat gaaaattaaa atcagactag gcgacgcaga tgcagataga
     3121 accctactac tttcacgctt tc
//
LOCUS       DASFSW010000182         3122 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.183, whole genome shotgun sequence.
ACCESSION   DASFSW010000182 DASFSW010000000
VERSION     DASFSW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.183"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..107
                     /locus_tag="U1099_002002"
     CDS             <1..107
                     /locus_tag="U1099_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEM4868279.1"
                     /translation="LQSQKKIEELHQLIDDKNKQNKKLQAISAAIDDL"
     gene            151..435
                     /locus_tag="U1099_002003"
     CDS             151..435
                     /locus_tag="U1099_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868280.1"
                     /translation="MKLFKKQTHNQDSFKRLIHRLSGMSEADLHKVNQLLDVVFDSTS
                     QTTNDVKPPKDLQKEPTLDETITEAKNKLKTEQLEKRIEQFRQAKKPKSE"
     gene            505..981
                     /locus_tag="U1099_002004"
     CDS             505..981
                     /locus_tag="U1099_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4868281.1"
                     /translation="MIGDNIKSLRKTHDLTQPEFAKIVGISRNSLSRYENGTSSLSTE
                     LIDRICQKFNVSYVDIVGEEKMLTPVEDYQLTLKIEVIKERGAAILSELYRYQDSQGI
                     TFDDETSPWILMSDDLSDLINTKIYLVNTFDEVERYNGYLDGIERMLELANHQVVA"
     gene            981..1775
                     /locus_tag="U1099_002005"
     CDS             981..1775
                     /locus_tag="U1099_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM4868282.1"
                     /translation="MRLEEFSKDDFDLALKRTIRSLTRGKTTSVTPKAILLGGQSGAG
                     KTTIHRIKQKEFQGNIIIIDGDSYRSLHPNYLTLQEEYGKDSVDYTKGFAGKMVEQLV
                     DELSKQGYHLLIEGTLRTTEVPRKTARLLKARGYQVSLALIATKPELSYLSTLIRYEE
                     VYVVNPNQARATPKEHHDGIVNHLVDNLRELENDHLFDYIQIYQRDRSCVYDSETDDG
                     SAAEVLQECLFGKWSKVEEEMMKMGQELLMEFLEMKKGRIYENKKN"
     gene            1756..1959
                     /locus_tag="U1099_002006"
                     /pseudo
     CDS             1756..1959
                     /locus_tag="U1099_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            2050..2994
                     /locus_tag="U1099_002007"
     CDS             2050..2994
                     /locus_tag="U1099_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM4868283.1"
                     /translation="MTYTHLTTNELVMIEAYYKEGIAVSHICQSLKRSRQTIHKVIAY
                     LKAGHTAYDYYKDYKANKKRCGRRQTQLTQSEQDFIQRHLELNWSLDVVKGTYPDKIP
                     CSMRTLYRLADRGIFKKVDLPWKGKRKPNGIAEKRGKQAFRRDIRERSEIYPDFDTEF
                     GHLEGDTIVGKHHKSAVVTLVEKQSKAIITLQTNGRKASDIEQSLNRWLSQFPKHLFK
                     SVTFDCGKEFSNWKTIANQHDIDIFFADPGCPGQRGLNEHSNGLLRRHGLPKQMDFNG
                     IPQKYLSAITDKRNRIPRKSLNYRTPYQVFLSYVKCLA"
BASE COUNT         1111 a          515 c          620 g          876 t
ORIGIN      
        1 ttcttcaaag tcagaagaaa atcgaagagt tgcaccagtt gattgatgac aaaaataagc
       61 aaaataagaa attacaagcc atttcagcag ctattgatga cttgtaagtg aggcagtcct
      121 tacaagggct gctttttata aggaaataag atgaagttat ttaaaaagca aacgcataat
      181 caagatagtt ttaagcgtct tattcatcgt ttatcaggaa tgtctgaagc cgacttacat
      241 aaggttaacc aattactaga tgtcgttttt gattcaacca gccaaacgac caatgacgta
      301 aaacctccta aagatttaca aaaggagcct accctagatg agactataac tgaagctaag
      361 aataaattga agactgagca actcgaaaaa cggatagagc aatttaggca ggcgaagaaa
      421 ccaaaaagcg aataacgcac catttttggt gcgttattcg ctttttgtgt tataataaaa
      481 gtaactaata ggaggacatt agcaatgatt ggagataata ttaaatcgtt acgtaaaacc
      541 catgatttaa cacaaccaga attcgcaaag attgttggaa tttctcgaaa tagtttgagc
      601 cgttatgaaa atgggacaag ctcgttatca acagagctca tagaccgtat ctgtcagaaa
      661 tttaatgtat catatgttga tattgtagga gaagaaaaaa tgttaacacc agtggaagat
      721 tatcaattaa ccttaaaaat tgaagttatc aaagagcgtg gcgcagcgat tttgtccgaa
      781 ctatatcgct atcaagatag tcaaggaatt acttttgatg atgagactag cccttggatt
      841 ttgatgagtg atgatttgtc tgatcttatc aataccaaga tttaccttgt caatactttt
      901 gatgaagtgg agcgttacaa tggttattta gatggtattg agcgaatgtt ggagcttgct
      961 aatcatcagg tggtggctta atgcggttag aagaatttag caaagatgac tttgacttag
     1021 ccttaaaacg aactattcgt tccttaactc gtggtaaaac gacatcagtt accccaaaag
     1081 ccatcttact tggtgggcaa agtggagcag gaaaaacaac gatacaccgt atcaagcaga
     1141 aagagttcca aggtaatatt atcattattg acggtgatag ctaccgctct ttacacccga
     1201 attatctaac tttacaagaa gagtacggga aggatagcgt ggattatacc aaaggttttg
     1261 caggaaaaat ggtggaacag cttgtggatg aactcagtaa acaaggttat catttgttaa
     1321 tcgaaggaac tttgagaacg acagaagtcc cacgaaaaac agcacggcta ttgaaagcga
     1381 gaggttatca agtctcatta gctttgatag caaccaagcc tgaattgtct tatctcagca
     1441 ccttaatccg ctacgaagaa gtctatgtgg ttaatccaaa ccaagctaga gcaactccta
     1501 aagaacatca tgatggaatt gtcaatcact tggttgataa cttacgagag ctagaaaacg
     1561 accatctctt tgattatatt caaatttatc aaagagatag aagttgtgtt tatgattcgg
     1621 aaactgatga cggttcagca gcagaagtgc tacaagaatg cctcttcgga aaatggagca
     1681 aggtggagga agagatgatg aagatgggac aggaattgtt gatggagttt ttggaaatga
     1741 aaaaaggtag aatttatgaa aataaaaaaa attaatgttc aaaattatcg tttattgaag
     1801 gatttttctt tagagttgaa gagtgaattg tcattaattg tgggaaaaaa taattgcggt
     1861 aaaacctcag ttttatctgt attggaaaag ataatcaata aaagttctag tttaacctgg
     1921 gaagatataa atttgtatca tagaaaagta atttttgaaa cctaatttgt aaaattaagc
     1981 tagacagaaa aaagccattt atgttaggct cagataaaca ccacatttgt ctgaaaggaa
     2041 ctaacataaa tgacctatac ccatcttacc acaaatgaac ttgtcatgat agaggcttat
     2101 tacaaagaag gaatagccgt tagccatatt tgtcaatccc tcaaaagatc aagacaaacg
     2161 attcataagg tcattgctta tctcaaggct ggccacactg cttatgatta ctacaaggac
     2221 tacaaagcta acaagaaacg ttgtggtcgt cgtcagaccc aactcactca aagtgaacaa
     2281 gattttattc aaaggcattt agaacttaat tggagtttgg atgttgtcaa aggaacttat
     2341 cccgataaaa tcccttgttc tatgagaact ctttatcgcc tagctgaccg tggtatcttc
     2401 aagaaagttg atttaccatg gaagggaaaa cgcaaaccaa atggtatagc cgaaaaacgt
     2461 ggcaaacaag cttttaggag ggatatccgt gagcgttcag aaatctatcc agattttgat
     2521 acagaatttg gacatcttga aggagataca atcgttggta aacatcacaa aagtgctgtt
     2581 gtcaccttgg tggagaagca atctaaagct attatcacac ttcaaaccaa tggtcgaaaa
     2641 gctagcgaca ttgagcagtc actaaataga tggctatcac aattcccaaa acatctcttt
     2701 aaatctgtca cttttgattg tgggaaagaa ttctctaatt ggaagacaat agccaaccaa
     2761 cacgacattg atattttctt tgcggatcca ggttgccctg gtcaacgtgg gttaaatgaa
     2821 cattcaaatg gtttattaag acgtcatgga ctaccaaagc aaatggattt caatggtatt
     2881 cctcagaaat atttatcagc tatcactgat aaacgaaata gaattcctag gaaatcttta
     2941 aattatcgaa caccatatca ggtttttctg agttacgtaa aatgtctagc ttaatttgac
     3001 aatttgggaa aatataaaaa aggttgcgta tacccctgac tcggaattag aacctatttt
     3061 aggaataaac cttcaaattt ggattcagta ctcagaagaa gattcttatc aaaacatcca
     3121 gc
//
LOCUS       DASFSW010000183         3077 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.184, whole genome shotgun sequence.
ACCESSION   DASFSW010000183 DASFSW010000000
VERSION     DASFSW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.184"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            30..659
                     /locus_tag="U1099_002008"
     CDS             30..659
                     /locus_tag="U1099_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEM4868284.1"
                     /translation="MIKEFCSENHVAVVKALSLGAQRVELCDNLAVGGTTPSYGVIKH
                     VCQLAHENEATVMTMIRPRGGDFCYDEAEVEMMVEDCKAAIELGADGLVYGLLTEENW
                     LDEPALERLFAVSQDCQIVFHMAFDQIPRERQFEALDWLVEHGVTRILTRGSLTGSAL
                     NNVEWLQEIVAYAQGKIEILIGGGLTVDNVPTLLEALPINQVHGTRLFW"
     gene            866..1240
                     /locus_tag="U1099_002009"
     CDS             866..1240
                     /locus_tag="U1099_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868285.1"
                     /translation="MNPLRKELRTVAVEVSDLALDYAVRLAQSLNSTLRYHNYDSLIA
                     IAKTKGVEPKGKDCQSFSEYRQRYSLYDAKKLIYRALAWRLFDDSHADYGHALTILGL
                     DEDESGVEQIGFAFSKFTLDID"
     gene            1424..1951
                     /locus_tag="U1099_002010"
     CDS             1424..1951
                     /locus_tag="U1099_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEM4868286.1"
                     /translation="MSLENYMPDFALEKAYDVTVESLKKHGIKVVFVDLDNTLIAWNN
                     PDGTPEMRQWLHDLQDAGIPVVVVSNNKYERVKRAVEKFGIEFEAFALKPFTFGINRA
                     LKRFDVQPYEVIMIGDQLMTDIRAAKRAGLKSVLVKPLIKTDSINTQINRWRERRTMK
                     KIIAKYGAIDYKREM"
     gene            1952..3058
                     /gene="yqeH"
                     /locus_tag="U1099_002011"
     CDS             1952..3058
                     /gene="yqeH"
                     /locus_tag="U1099_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEM4868287.1"
                     /translation="MEELFCIGCGAQIQTLDKAVAGFTPQSALEKGLETGQLYCQRCF
                     RLRHYNEISDVNISDDDFLKLLHSVGESDALVVNVIDIFDFNGSVIPGLPRFISGNDV
                     LLVGNKQDILPKSVKTGKVTQWLTERAHEIGMRPVDVVLTSAQNKQAIKDLIEKIEQH
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYISPRKEIKPKTYQLNPEQTLFLAGLGRFDFVAGERQ
                     GFTAFFDNELQLHRTKLQGASEFYQKHAGSLLVPPTSKELKEFPELVRHEFTINEKTD
                     VVFSGLGWIRVNEKAKIAAWAPKGVDVVIRKAII"
BASE COUNT          874 a          566 c          749 g          888 t
ORIGIN      
        1 taaagaaaaa agacttgaaa ggaataaaga tgattaaaga attttgttcc gaaaatcatg
       61 tagctgtggt gaaagctctt tctctgggtg ctcaaagagt tgaactgtgt gataatttag
      121 cggttggcgg aacgacgccg agttatggag tgatcaaaca tgtttgtcaa ctggctcatg
      181 agaatgaagc cacagtcatg accatgattc gccctcgcgg tggagatttt tgctacgatg
      241 aagcagaggt ggaaatgatg gttgaagatt gtaaagcagc gattgaactg ggggcagatg
      301 gtctggttta tggtttgtta accgaggaaa attggttgga cgaaccagcc ttggaaagac
      361 tgtttgctgt ttcgcaggac tgtcagattg tctttcacat ggcttttgac caaattcctc
      421 gcgaacgcca attcgaggct cttgattggc tggtagaaca tggagtgaca cggattttga
      481 cgcgtgggag tctgacaggg tctgccttga acaatgtgga atggttacag gaaattgtcg
      541 cctatgcaca aggtaaaatt gagattttga ttggtggagg attgactgtg gacaatgtgc
      601 caacgttatt agaagcatta ccaatcaatc aagtacatgg aacgagatta ttttggtaag
      661 gtagctttga aagtgaaata aattacagag taataatcga agtttattca gaaatgttta
      721 gaagttaagg tttgtttaag cattggaaga tacaatatat ttattctttt tcatataatc
      781 tccacggaag cagtcgacat gttcggctgt tttcattttt gtttgttaaa gtccggtata
      841 cagactagaa actgctgcgt ctctattgaa tcccctacgt aaagaattga gaactgtggc
      901 tgttgaggtg agtgatttag ctttggacta tgctgttcgt ttagcccaat ccttgaatag
      961 tactttgcgt tatcataatt atgatagctt gattgccatt gctaaaacca aaggagtaga
     1021 accgaaaggt aaggattgtc aatcattttc agagtacaga cagcgttaca gtctctatga
     1081 tgctaagaag ttaatttatc gcgctctcgc gtggcgtcta ttcgatgata gtcatgcaga
     1141 ttatggacat gctcttacca ttttggggtt ggatgaggat gagtcaggag tagagcaaat
     1201 cggttttgct ttttcaaaat ttactcttga tattgattga ttgctaaccc atatgatttt
     1261 cattccgaaa gattggattt tggaagaagg tcagatttaa ttcacaacta ccattacatt
     1321 acctctagaa aacactcggt tgatccgagt gttttctgct atcgattcgc atttttctta
     1381 gaaaaatggt ataattatct gataaaaaac tttggagacg acagtgagtt tagaaaatta
     1441 catgccggat tttgccttgg aaaaggctta tgatgtgacc gtcgaaagct tgaaaaaaca
     1501 tggcataaaa gtagtgtttg ttgacttgga taataccttg attgcttgga ataatcccga
     1561 tggtacgcca gagatgcgcc agtggttaca tgatttgcag gacgcaggta ttcctgttgt
     1621 ggtggtgtct aacaataaat acgaacgtgt caaacgggcg gttgaaaaat ttgggattga
     1681 atttgaagcc ttcgctctca agcctttcac ctttgggatt aaccgtgctt tgaaacgctt
     1741 tgatgtccag ccgtatgagg taattatgat tggcgatcag ctgatgacgg atattcgggc
     1801 tgctaagcga gctggtctca agtctgtctt ggtcaaaccc ttgataaaga cggactctat
     1861 caatacgcag attaaccgtt ggcgtgagcg acggactatg aaaaaaatca tcgctaaata
     1921 tggagcgata gactacaaga gggagatgta agtggaagaa ttattttgta tcggctgtgg
     1981 tgcccagatt cagacgctag acaaggctgt cgctggtttt acacctcaat cagccctaga
     2041 aaaaggcctg gaaacagggc aactctactg ccaacgttgt ttccgtttgc gccattacaa
     2101 tgagatttca gatgtcaata tctcagacga tgatttcttg aaactcctcc atagcgtagg
     2161 agaaagcgat gccttggtgg ttaatgtcat tgatattttt gatttcaatg ggtcagtcat
     2221 tccaggttta cctcgcttta tttctggaaa tgacgtgctt ttggtcggca acaagcagga
     2281 cattttaccc aagtctgtca agacaggcaa ggttactcag tggttgaccg agcgggcaca
     2341 tgaaatcggc atgcgaccgg ttgatgtggt cttgacttca gcccaaaata agcaggctat
     2401 caaggatttg attgaaaaga ttgaacaaca ccgcaaggga cgggatgtct atgtggtcgg
     2461 cgtgaccaat gtcggcaagt caaccttgat taacgctatt attcaagaaa tcacagggga
     2521 caaggatgtc attacgactt cacgtttccc tggaacgacg ctggataaga ttgaaatccc
     2581 tctggatgat ggttcataca tctatgacac gccaggaatt atccaccgtc accagatggc
     2641 ccattatttg acggctaaaa acctcaagta catcagccct cgcaaggaaa tcaagccaaa
     2701 gacctaccag ctcaatccag aacaaacctt gttcttggct ggtctgggac gatttgactt
     2761 tgtggctggt gaacgccaag gctttacggc cttctttgat aatgagctcc aactccaccg
     2821 caccaagttg cagggagcta gcgagtttta tcagaaacat gcgggctcac ttttagtgcc
     2881 accaaccagc aaggaattga aagaatttcc tgaactggtc cgtcatgaat ttaccatcaa
     2941 cgagaagacg gatgtggtct tctcaggact tggttggatt cgtgtcaatg aaaaagccaa
     3001 gattgctgcc tgggctccta agggtgtgga tgtggtcatt cgcaaggcga ttatctaata
     3061 ctcttcgaaa attaaaa
//
LOCUS       DASFSW010000184         3034 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.185, whole genome shotgun sequence.
ACCESSION   DASFSW010000184 DASFSW010000000
VERSION     DASFSW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.185"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>3034
                     /locus_tag="U1099_002012"
     CDS             <1..>3034
                     /locus_tag="U1099_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEM4868288.1"
                     /translation="FPEDLTDFYPKTTRDKVETNVAAIRLVKALESENRQATPSEQEL
                     LAKYVGWGGLANEFFDEYNPKFSKEREALKTLVTDKEYSDMKQSSLTAYYTDPHLIRQ
                     MWEKLERDGFTGGKILDPSMGTGNFFAAMPKHLRENSELYGVELDTITGAIAKHLHPN
                     VHIEVKGFETVNFNENSFDLVLSNVPFANIRIADSHYDKPYMIHDYFVKKSLDLVHDG
                     GQVAIISSTGTMDKRTENILQDIRETTDFLGGVRLPDTAFKAIAGTNVTTDMLFFQKH
                     MDKGYVADDLAFSGSIRYDKDDRIWLNPYFDGDYNSQVLGSYEVRNFNGGTLSVRGTS
                     DNLLADVQTALKQVKAPRTVDPSDILITPDVVRRQVIDTSIPSDIRESLDRYSFGYKD
                     SMVYYRDNKGIRVGTKTEEISYYVDEEGAFKGWDTKHSQKQIDRFNDLEVTDSTALDV
                     YVTEEATKRGQFKGYFKKTVFYEAPLSEKEVARIKGMVDIRNAYQEVIAIQRYYDYDR
                     DEFNHLLGHLNRTYDTFVKRFGYVNSAVNRNLFDSDDKYSLLASLEDESLDPSGKTVI
                     YTKSLAFEKALVRPEKEVMAVSSALDALNSSLADGRGVDLDYMMSIYQTDSKASLIEE
                     LGDAIIPDPERYLNDREVVYVSRQDFLSGDVVTKLEVVDLLIKEDNSDFPWVYYQGLF
                     EDVKPPRVTLADIDYRIGSRWIPLAVYGKFAQETFMGQTFDLTDQEVATVLEVSPIDG
                     TMSYQSKFAFRYSTATDRSLGVPGSRYDSGRKIFENLLNSNQPTITKQVEDGDKKKHV
                     TDVEKTTVLRAKETQLQELFQDFVASYPEVQQMIEETYNSLYNRTVSKVYDGSHLTID
                     GLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYV
                     VPSSLTAQFGQEIMKFFPTKNVYVTTKKDFAKSKRKQFVSRIITGDYDAIVIGDSQFE
                     KIPMSHEKQVTYIQDKLQQLRDIKQGSDSDYTVKEAERS"
BASE COUNT          891 a          589 c          707 g          847 t
ORIGIN      
        1 acttcccaga agatctaaca gacttttacc ctaagacaac aagagataag gttgaaacca
       61 acgtggctgc cattcgtctt gtaaaagctc ttgaatctga aaaccgtcaa gccacaccta
      121 gtgaacaaga actccttgcc aaatacgtgg gctggggagg acttgctaat gaattttttg
      181 acgagtataa cccaaagttt tctaaggaac gtgaggcatt aaaaacgcta gtcaccgata
      241 aagagtattc agacatgaaa caatcttctc tgactgccta ttacacagac cctcacttga
      301 ttcgtcagat gtgggagaag ttagaacgtg atggctttac aggtggtaaa atcctagacc
      361 cgtctatggg aacggggaat ttctttgcgg caatgcctaa acacctgaga gaaaatagtg
      421 agttgtatgg tgtagagtta gacacgatta caggggcaat tgctaaacac ctccatccca
      481 acgtccacat tgaggtgaaa ggctttgaaa cagtcaattt taatgagaat agttttgatt
      541 tggttctttc aaatgtgcct tttgccaata tcaggattgc ggacagtcac tatgataaac
      601 cctatatgat tcatgattac tttgttaaaa aatcattaga tttggtgcat gatggtgggc
      661 aagtggctat catctcctcc acaggaacca tggacaaacg gacagaaaac attctccaag
      721 atattcgtga gacgactgat tttcttggtg gtgtgcgttt gccagatact gcctttaagg
      781 ccattgcagg caccaatgtc acaacggaca tgttgttctt ccaaaagcat atggataagg
      841 gatatgtggc agatgattta gctttttcag gttctattcg ctatgacaag gatgaccgta
      901 tttggctgaa tccatatttt gacggagact acaacagtca ggttcttggg agctatgagg
      961 ttagaaactt taacggtggg acactttctg tgagaggaac ttctgataat ttgctagcag
     1021 atgttcagac tgctcttaaa caggttaaag cgcctcgaac tgttgaccct tctgacatcc
     1081 ttatcactcc tgatgtggtg agaagacaag ttattgatac ctcgattcct tctgacatca
     1141 gagaaagctt agatcgttat agttttggtt ataaggattc gatggtttac taccgagata
     1201 ataagggaat ccgtgttggg actaagactg aggagattag ctactatgtg gacgaggagg
     1261 gggcctttaa gggttgggat accaagcatt cgcaaaaaca gattgaccgc tttaatgacc
     1321 tcgaagtcac cgatagcacc gctcttgatg tgtatgtgac cgaagaagcc accaaacgtg
     1381 ggcagtttaa gggctatttc aaaaagacgg ttttctatga agcgcctttg tcagagaaag
     1441 aagtggcacg gattaaaggg atggttgata ttcggaacgc ctaccaagag gtgattgcca
     1501 ttcagcgcta ctatgattac gacagagatg agtttaacca cttgttagga catctcaatc
     1561 gcacctatga tacttttgtg aaacgctttg gttatgtcaa tagtgctgtg aatcgtaacc
     1621 tttttgatag tgatgataag tattcgctcc ttgcgagttt agaagatgaa agtcttgacc
     1681 cgagtgggaa gacagttatc tataccaaat ctcttgcttt tgaaaaagcc ttggttcgtc
     1741 ctgaaaagga agtgatggca gtttcttctg cccttgacgc tctcaattct agtctagcag
     1801 atggtcgtgg ggtagatttg gattacatga tgtctattta tcagacggat tctaaagcgt
     1861 ctttgattga agaattaggt gacgctatta ttccagaccc tgaacgctat ctaaacgata
     1921 gggaagtggt ctatgtgtct cggcaggatt tcttatcagg agatgtggtg actaagttag
     1981 aggttgtgga cttactcatc aaagaagaca actctgattt tccttgggtc tattatcaag
     2041 gtctctttga agacgtgaaa ccaccacgtg tgaccttagc tgatattgat tatcggattg
     2101 gctcacgttg gattcctcta gccgtttatg ggaaatttgc ccaagaaacc tttatgggac
     2161 aaacctttga cctgactgac caagaagtag caactgttct tgaagtcagt ccgattgatg
     2221 ggacgatgtc ttaccaatcc aagtttgcct ttaggtattc tacagcaacg gatagaagtt
     2281 taggtgtgcc tgggtctcgg tatgatagtg gtcgtaaaat ctttgaaaac ctgcttaact
     2341 ccaatcaacc aaccatcacc aaacaagtgg aagatgggga taagaaaaag catgtgacgg
     2401 acgtggagaa gacaacggtt cttcgtgcta aggaaacaca acttcaagag ctttttcaag
     2461 actttgtggc cagttaccca gaggtacaac agatgattga agagacctat aatagccttt
     2521 ataatcgtac agtttcaaaa gtctatgatg gcagtcattt aaccattgat gggcttgccc
     2581 agaatatttc cttacgccct caccagaaga atgctattca acgcattgtg gaggaaaaaa
     2641 gagcgcttct agctcatgaa gttggttcag gtaagacctt aaccatgctt ggagcaggct
     2701 tcaaattgaa ggagctcggt atggtgcata aacctcttta tgtggtgcca tcaagtctga
     2761 ccgctcagtt tggccaagaa atcatgaagt tcttcccaac caagaatgtc tatgtgacga
     2821 ccaagaaaga ttttgcgaaa tccaaacgca agcaatttgt gtcacggatt atcacagggg
     2881 actacgatgc cattgtcatt ggggattccc agtttgaaaa aatccctatg agccatgaaa
     2941 agcaggtgac ttatatccaa gataagttac aacaactccg tgacatcaag caaggcagtg
     3001 atagtgacta taccgttaaa gaagcagagc gttc
//
LOCUS       DASFSW010000185         2990 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.186, whole genome shotgun sequence.
ACCESSION   DASFSW010000185 DASFSW010000000
VERSION     DASFSW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.186"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            14..526
                     /locus_tag="U1099_002013"
     CDS             14..526
                     /locus_tag="U1099_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEM4868289.1"
                     /translation="MIVLITGASHTGKTVLAQKLLEKYHYPYLSIDHLKMGLIRSGNT
                     KLSVEEDDKLTAYLWPIVREMIKTAIENEQNLIVEGIYIPFDWATDFAIAYLDHIRYY
                     CLVMSENYIQKHFGSIKKHARLIENRIDDEWCTMESVLEENTRFFRRAKKYDANILWI
                     DESYEIEIDL"
     gene            873..1649
                     /gene="rpsB"
                     /locus_tag="U1099_002014"
     CDS             873..1649
                     /gene="rpsB"
                     /locus_tag="U1099_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEM4868290.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFIRDAAANDAVILFVGTKKQAAEAVKDEAIRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEINRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIADMPRIPDV
                     MFVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKMA
                     DAIIEGNQGEDSVAAVEAELAAEPASTESIEELVEVVEGK"
     gene            1902..2942
                     /locus_tag="U1099_002015"
     CDS             1902..2942
                     /locus_tag="U1099_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEM4868291.1"
                     /translation="MAEITAALVKELREKSGAGVMDAKKALVETEGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVVEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPADNEAALKLAMPSGETLEEAYVNATATIGEKISFRRFALVEKTDAQAFGAYQHNG
                     GRIGVISVVEGGDETLAKQISMHIAAMKPTVLSYTELDEQFVKDELAQINHKIEQDNE
                     SRAMVDKPALPLLKYGSKAQLTDEVIAAAEEAIKAELAAEGKPEKIWDKIIPGKMDRF
                     LLDNTQVDQAYTLLAQVYIMDDSKTVEAYLNSVNASVVEFARFEVGEGIEKASNDFEA
                     EVAATMAAALGK"
BASE COUNT         1027 a          539 c          656 g          768 t
ORIGIN      
        1 ataggagaaa atcatgattg ttttaataac gggagcatct cacactggga agactgtgct
       61 ggctcaaaaa ttacttgaaa aatatcacta tccttattta tcaatagacc atttgaaaat
      121 ggggttaatt cgtagcggca atactaaact ttcagtcgaa gaagatgata aacttacagc
      181 atatctatgg cctattgttc gtgaaatgat taaaactgcc atagaaaatg agcaaaacct
      241 tattgttgaa gggatctata tcccttttga ttgggcaacc gactttgcaa tagcctacct
      301 tgaccatatc agatattact gtcttgtgat gagtgaaaac tatattcaga aacactttgg
      361 aagcataaag aaacacgcac gccttataga aaatcgtata gatgatgaat ggtgtacgat
      421 ggaaagtgtt ttagaagaaa atactcgatt ttttagaagg gcgaaaaaat atgatgcaaa
      481 catcctatgg attgatgaga gttacgagat agagattgat ttatgataga gagacattgt
      541 atcacagttt atctaaggga tacaactaag aggaagtaac atcagatagg cgttacttcc
      601 tttttatttc taagaaatat aaagtaaaat ggatgaagtt aaaaatcata aaaaagtagt
      661 aacataaaga gagacatatg ccaatctatg agcttggcat aatatagaga tttgtgtact
      721 gaaatgcttg attttcacct aaaactttgg tatacttact aagttgtcta aacaacaaat
      781 actcatctcg gaccaattgt ggtcttgttg ccgaaaggtt gggctgcaag tcgaagttcg
      841 ggagaggaga aaaaacaaaa ggagaaatac tcatggcagt aatttcaatg aaacaacttc
      901 ttgaggctgg tgtacacttc ggtcaccaaa ctcgtcgctg gaatcctaag atggctaaat
      961 acatcttcac agagcgtaac ggtatccacg ttatcgactt gcaacaaact gtaaaattgg
     1021 ctgaccaagc ttacgaattc atccgtgacg ctgcagcaaa cgacgcagtt atcttgttcg
     1081 taggtactaa aaaacaggct gctgaagctg ttaaagacga agctatccgc gctggtcaat
     1141 acttcatcaa ccaccgttgg ttgggtggaa ctcttacaaa ctggggtaca atccaaaaac
     1201 gtatcgctcg tttgaaagaa atcaaccgta tggaagaaga tggaactttc gaagtgcttc
     1261 ctaagaaaga agtagcattg ttgaacaaac aacgtgctcg tcttgaaaaa ttcttgggcg
     1321 gtatcgctga catgccacgc attccagacg taatgttcgt agttgaccca cataaagagc
     1381 aaatcgctgt taaagaagct aaaaaattgg gtatcccagt tgtagcgatg gttgacacaa
     1441 acactgatcc agatgatatt gatgttatca tcccagctaa cgatgacgct atccgcgctg
     1501 ttaaattgat cacagctaaa atggctgacg ctatcatcga aggcaaccaa ggtgaagaca
     1561 gcgtagcagc agttgaagct gaattggcag ctgagccagc atctacagaa tcaatcgaag
     1621 aattggttga agttgtagaa ggaaaataag taacgaaggc gtttggaaat ctgtatgaaa
     1681 ataggaaact gacgcagtat gcttgcatac aaggaagttt atctttttca ctaagatttt
     1741 agcctgagta caattgaata agtaatgaag gcgaattaaa atcaatacgg ttttagctcg
     1801 cgtataattc tacatcatta tttaacaaca aaaaaacaat cctagagggg cagggctgag
     1861 cccgctcctc tattttataa atacaaacaa ggagaataga catggcagaa attacagcag
     1921 ctctcgttaa agaattgcgt gaaaaatcag gtgctggcgt tatggacgca aaaaaagcat
     1981 tggttgaaac tgaaggtgat atcgaaaaag cgattgaatt gcttcgtgaa aaaggtatgg
     2041 ccaaggcagc taagaaagct gaccgtgtag cagctgaagg tttgacaggt gtttacgttg
     2101 atggtaacgt agcagcagtt gttgaagtga acgctgaaac agacttcgtt gcgaaaaacg
     2161 ctcaattcgt tgagttggta aacactactg ctaaagtaat tgcagaaggc aaaccagctg
     2221 acaacgaagc agctcttaaa ttggctatgc cttcaggtga aacccttgaa gaagcatacg
     2281 taaacgcaac tgcaactatc ggtgagaaaa tctcattccg tcgttttgct ttggttgaaa
     2341 aaacagatgc tcaagcattt ggtgcatacc aacacaacgg cggccgtatc ggtgttatct
     2401 cagttgttga aggtggcgac gaaactcttg caaaacaaat ctcaatgcac atcgctgcga
     2461 tgaaaccaac tgttctttct tacactgaat tggatgagca attcgttaaa gatgagttgg
     2521 cacaaatcaa ccacaaaatc gaacaagaca acgaaagccg tgcaatggtt gacaaaccag
     2581 cattgccatt gttgaaatac ggttcaaaag ctcaattgac tgacgaagtg attgcagcag
     2641 ctgaagaagc tatcaaagca gaattggcag cagaaggcaa accagaaaaa atctgggaca
     2701 aaatcatccc aggtaaaatg gatcgcttct tgcttgacaa cacccaagtt gaccaagctt
     2761 acacacttct tgcacaagtt tacatcatgg acgacagcaa aacagttgaa gcttacttga
     2821 actcagtaaa tgcttcagtt gtagaatttg ctcgtttcga agtaggtgaa ggtatcgaga
     2881 aagcttcaaa cgactttgaa gcagaagttg cagcgacaat ggcagcagct cttggtaaat
     2941 agtagatagt taaaaaagct accttagaag gtagcttttt ctgtgggctt
//
LOCUS       DASFSW010000186         2972 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.187, whole genome shotgun sequence.
ACCESSION   DASFSW010000186 DASFSW010000000
VERSION     DASFSW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.187"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            23..196
                     /locus_tag="U1099_002016"
     CDS             23..196
                     /locus_tag="U1099_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868292.1"
                     /translation="MKIKLFKKLPKESLKDFEERVNEFMATVEVVDVKITMAPAGHSD
                     SFGTVTHILVLYK"
     gene            217..792
                     /locus_tag="U1099_002017"
     CDS             217..792
                     /locus_tag="U1099_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868293.1"
                     /translation="MTLKTFSDTPNTFTFNYTFKDHDTAQVAGHALMGYMTGTFEQPA
                     IEVHYKSDKVGGDYNRLAVEYVADTELTETFQRICDSFQDYYNNPDELTGEDLDDYEQ
                     VQEIEQEYIRQRVEQLKQSESFDSLLEKVAGLELELMELADSVLDDDYPDMAVNGVWE
                     NMTAVEDEARKLLKELDTEDNYCALWKYSAE"
     gene            840..1241
                     /locus_tag="U1099_002018"
     CDS             840..1241
                     /locus_tag="U1099_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868294.1"
                     /translation="MIKNKGGRPTKMTQGTVKKLEEAFLRGLSDEEACLYANISKPTL
                     YDYCKKNPQFSDRKELLKQRLKTRAKLNISNAIEDGDVVISKWYLERKDDEFKTKTKL
                     EHDGNVSVSPHNPFEDLTVEELRALIAEDEG"
     gene            2076..2495
                     /locus_tag="U1099_002019"
     CDS             2076..2495
                     /locus_tag="U1099_002019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868295.1"
                     /translation="MDLKQLAKFEKLVQSKKREIEALRAGIVNTPDYQSQRVTRSKKN
                     TTEDQVIKRIEKIEQLETELAQLYADYSGLSARIDAITDPVASLIMRLKYINGYSWLM
                     IKRTLPYYSQSSLYDYHKKGLEELEQTTSKKKTERVS"
     gene            2506..2796
                     /locus_tag="U1099_002020"
     CDS             2506..2796
                     /locus_tag="U1099_002020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868296.1"
                     /translation="MAVLHYDMIQVDENWIVKLFEFDPEDYKGRIYDWQRKAPEEVNE
                     IIKAVNAIARPRHRAILIMSYISPEKIRSAEQAQRLGIKSSTYYLAKNIALP"
BASE COUNT         1022 a          531 c          694 g          725 t
ORIGIN      
        1 ttatcttaaa gtgagggaaa acatgaaaat taaactattt aaaaaattgc ccaaagaaag
       61 cctgaaagat tttgaagagc gagtaaatga gttcatggca accgttgaag tggttgacgt
      121 aaaaatcact atggcaccgg cggggcattc tgacagcttt ggcacagtaa cacatatact
      181 agtcttatac aaataacaga aacggagaat gataacatga cattaaaaac attttcagac
      241 acaccaaaca catttacatt taactacacc ttcaaagacc acgacaccgc acaagttgca
      301 gggcacgcgc ttatgggcta catgacagga acatttgaac aaccagctat cgaagtgcat
      361 tataagagtg acaaagtggg cggagattac aaccgtttgg cggtggaata tgtggcagat
      421 actgaactta ctgaaacatt ccagcggatt tgtgacagtt tccaagatta ctacaacaat
      481 cctgatgagt taacaggcga agacttggac gactacgaac aagtacagga aatcgaacaa
      541 gagtatatcc gtcagcgcgt ggaacaactc aagcagtcag agagctttga cagcttactt
      601 gaaaaagtgg ctgggttaga gcttgaactg atggagctag ctgacagcgt gctagacgat
      661 gattatcctg atatggcagt gaatggggta tgggagaata tgacggccgt agaggatgaa
      721 gcaagaaagt tactgaagga gcttgatacc gaagataact attgcgcttt gtggaagtat
      781 tcagcagaat agcagagagg cggttgcctc tttttgtgct aaaattagag aggaataaca
      841 tgataaaaaa taaaggtggc agacctacaa aaatgacaca aggaacggta aagaaattag
      901 aggaggcatt tttaagaggt ttaagcgacg aagaagcctg cttatatgcg aatatctcaa
      961 agccgacact atacgattat tgcaagaaaa atccacagtt ttctgaccga aaagagctac
     1021 ttaagcaacg actgaaaaca cgcgcaaagt tgaacatatc taacgccata gaggatggag
     1081 acgtggttat ttccaaatgg taccttgagc ggaaagatga tgaatttaaa accaagacaa
     1141 aactagaaca tgatggcaat gtgtctgtat cgcctcacaa tccatttgaa gatttaaccg
     1201 tagaggaatt acgggcattg attgctgaag atgaaggata aaccaatagt tttgggtttt
     1261 tacagtatag gggtattttt gaatggaatt attgtatata gagacattta ccagagctat
     1321 gaaaaagaaa ggaataaccc agggtaaggc tgaattagac ggttacccct tgactattga
     1381 cttagacaac ctttttattg ttatgggtag agatcgtttt gaattggcca gaataccagg
     1441 aacaaaaggc ggttataggt atttctttct ttgccctgat tgcgggaggc gttgcaggaa
     1501 actatataag cggtgtctat attttagttg tgggacgtgt cagcagatcc ataagcagac
     1561 actaaaccgc agtaagacag attgccaata ttattgggaa cgtgcactaa gggaggctag
     1621 gaaggtccaa ccagggtggg cccctgaacg tggcggatat atgtttgatg gctttcctag
     1681 ccgtccaaag agaatgaagc gtgatagata ttataaacat tatcaaaggt ttttgaacta
     1741 tgttagaaaa ggggatagct attggctgag agggttatct aggtgaagta ctgtgtcaat
     1801 tttcaaaacg aacaaatact acaaacgttc gctttacaaa acaaataaag gcggaacggt
     1861 tgggagctaa aaagctagta ctatcaagca ttcacagggt tgaaagggtg caaaaatacg
     1921 gaacgttttt tgatttttca aaacgacgaa tatccgaacg ctttataatt ctaaaagacg
     1981 gtggcgcgtg attacacaaa aaaaagccaa tgcgctcgca ctgactttgt gagtaaaaac
     2041 ctaaaactat tatatcacag ttggaggtgc gtagcatgga tctaaaacag ttggctaagt
     2101 ttgaaaagct ggtacagtca aagaaacgag agatagaagc cttgagggct ggcattgtca
     2161 atacaccaga ctatcaaagt cagagggtaa cacgatccaa gaaaaacact acagaagacc
     2221 aggttataaa acggattgag aagattgagc aactagaaac tgaactagca cagttgtatg
     2281 ctgattacag tggactatcc gcccgcatcg atgcaatcac cgacccagtt gcaagcctga
     2341 taatgcggtt gaaatacata aacggctatt cttggttaat gattaagcgg acattgccat
     2401 actactccca gagtagtcta tacgattatc acaagaaagg actggaagag cttgagcaaa
     2461 caactagcaa aaagaaaact gaaagagttt catagatggt gcagggtggc agttctgcat
     2521 tatgacatga tacaggtaga tgagaattgg attgtgaaac tatttgagtt tgaccccgaa
     2581 gactacaagg gcaggatata tgactggcaa cgtaaagccc ctgaagaagt gaatgagatt
     2641 ataaaggcag tcaatgcaat agctagacca agacatcgag ccatacttat catgagttat
     2701 atatcacccg aaaagatacg atcagcagag caggcacaac gacttggcat taagtcatct
     2761 acttactact tggctaagaa tatagcctta ccgtagtgga gaactactgc agtatctggg
     2821 cgggtaaccc tgcctaccct acttaccgcc cttcacgccg tcattgcgca aattttttcg
     2881 cgggaaatat gctattcttc aattttgcct attttgagca aatagctacc acgcgccata
     2941 gtctatttca caaaaatggt aattttgaac gg
//
LOCUS       DASFSW010000187         2941 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.188, whole genome shotgun sequence.
ACCESSION   DASFSW010000187 DASFSW010000000
VERSION     DASFSW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.188"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..282
                     /locus_tag="U1099_002021"
     CDS             <1..282
                     /locus_tag="U1099_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868297.1"
                     /translation="QNVETLLLDANGRRIIANSEFMILLKQAKSDREELVHLLGLSKE
                     LEKYLVNPEKGAGLIKAGSTVVSFRNKIPLQTQLFDIMSTDPEKMRGES"
     gene            279..>2941
                     /locus_tag="U1099_002022"
     CDS             279..>2941
                     /locus_tag="U1099_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEM4868298.1"
                     /translation="MTREQQKVKVARKTFQSNLKASRIHYRREKKGLKRSLPKRRFIM
                     RRAEKAETREQRQALKQTYQEEKDLATDTFKEAIAYVSPRWLKAREIKKYRLPQARQR
                     LAVARKHLAEVKMAEKEEAVTSKVTDQKKPTESKTSRFQFQKEKPLERLQADKEVKSA
                     KRDVKQLKKAHQFKNPSTKVKRGLRYVASESLDVVAQVDDLEGIRTLKESVIKGRRYG
                     RFTYQSGKLLVKSGQTGLRFTKTKAAHSRERYQNFKKGKGFTRQKPLKPKRRYHTFLK
                     QARRHSVSGLRGVIQAIKNSLTFFSSIALNPMTWVVSGLLFFLLLMMSFVIGISGTTL
                     IQQDESELTKAYTHMTWEDAENTRTNPTGITYYTKIDDVMGFMNLKYQDYALEEVMEE
                     GDKTYQAYLSQLWQDLNGGDSLKTMLELSKEPAYKLSDDDREDLKELSEEGTYLALQE
                     LDNPFQDQTEDDALTMTVRYGYEVIDDKPTLHHHIILEAKENQVIVAPMDGKVSLDGE
                     NIIITSGKGLNKSQLTLFNIHTGRVSDGQKVQAGEVIGQTKDGAGLKVTYQKVDDDSE
                     KLVYVNPAFYFPKVIQLQTTILPTIGQFGGDEFARAKAIYEYLKSQGATNQAIAAILG
                     NWSVESSINPKRAEGDYLSPPVGATDSSWDDEGWLSLNGPAIYNGRYPNILRRGLGLG
                     QWTDTADGSRRHTLLLEYTKRKNQKWYDLGLQLDFMLNGDNPYYTKWLKDFFNNSGSP
                     ASLAQVFLIYWEGNSGDKLLERQTRATEWYYQIEKGFSQPNGGTAQSDPKALEAVRGD
                     LYDNSIPGGGDGMGYAYGQCTWGVAARINQLGLKLKGRNGEKIPIISTMGNGQDWVAT
                     AARLGGDTGKLPKAGAIISFAGGGHGSPTEYG"
BASE COUNT          935 a          583 c          715 g          708 t
ORIGIN      
        1 caaaacgtag aaaccttact tcttgatgcc aacgggagac gtattatcgc taatagcgag
       61 ttcatgatac ttctaaaaca agctaaaagt gacagagaag agttggtcca tctcttgggc
      121 ttatctaagg agcttgagaa atatttggtt aacccagaaa agggagctgg attgattaag
      181 gcaggctcaa cggttgtttc ctttagaaat aaaatcccac ttcaaaccca actctttgac
      241 atcatgagta ccgatccaga aaagatgagg ggtgaatcat gacaagagag caacagaagg
      301 tcaaggttgc ccgtaaaacc tttcagagta atttaaaggc cagccgtatc cattatcgca
      361 gggagaagaa ggggttaaaa cgctcacttc ctaagcgacg ctttatcatg cgccgagctg
      421 agaaagctga aacaagggaa caacgtcagg ctctgaagca aacctatcag gaggagaagg
      481 acttagcaac agataccttt aaggaagcga ttgcctatgt gtctcccaga tggctaaagg
      541 ctagggaaat taagaagtat cggcttcctc aagccagaca gcgcctagca gtagcaagaa
      601 aacacttggc agaggtcaag atggccgaaa aagaggaggc tgttacctct aaggttaccg
      661 atcagaaaaa gccaacagaa tcaaaaacat cacggtttca gtttcaaaaa gaaaaacctc
      721 ttgaacgcct tcaggcagac aaagaggtca aatcagcgaa gcgtgacgtg aaacaactga
      781 aaaaagcaca tcagtttaag aacccctcga ctaaggtcaa acgagggtta cgctatgtcg
      841 cttcagaatc acttgacgta gtcgctcaag ttgatgactt ggagggaatc agaaccctaa
      901 aagagtctgt gattaaagga agacgctatg ggagatttac ttatcagtca gggaaactcc
      961 ttgtcaaaag tgggcagact ggtcttcggt ttactaagac gaaagccgct catagtaggg
     1021 agcggtatca gaactttaaa aagggtaaag ggttcacccg acagaagcct ttaaagccca
     1081 aaaggcgata tcacaccttt ctcaagcaag ccagaaggca cagtgtgtca ggactcagag
     1141 gggttattca agcgattaag aatagcctga ccttcttttc atcgattgcc cttaatccta
     1201 tgacttgggt cgtgtcaggt ctcttgtttt tcttgctttt gatgatgagt tttgtgatag
     1261 gaatttcagg aactactctc attcaacagg atgagagtga actgacaaaa gcctatacgc
     1321 acatgacgtg ggaagatgcg gaaaacaccc gaaccaatcc aactggcatt acctactaca
     1381 ccaagattga tgacgtcatg ggctttatga atctcaagta tcaagattat gctttagaag
     1441 aagtcatgga agaaggggat aaaacttatc aagcctatct cagtcagctt tggcaggact
     1501 taaacggtgg agattcttta aaaaccatgc ttgagttaag taaggaacct gcctataagc
     1561 tctctgatga cgatagagaa gacttaaagg aattaagtga ggaaggcacc taccttgccc
     1621 tccaagaatt ggacaatcct tttcaagatc agactgaaga cgatgcgctt accatgacag
     1681 tacgctatgg ctatgaggtg attgatgaca agccaacgct tcatcatcat atcattctag
     1741 aagctaaaga aaatcaagtg attgtggctc ccatggatgg taaggtttcc ctagatggcg
     1801 agaacattat catcacgtca ggtaagggac taaacaagtc tcaattgacc ttgtttaata
     1861 tccatactgg tcgagtgagc gatggccaaa aggtacaagc aggagaggtg attggtcaaa
     1921 caaaggatgg tgctggtctt aaagtgacct atcaaaaggt tgacgatgat tcagagaaac
     1981 tggtttacgt caatccagcc ttttactttc ctaaagtcat tcaactccaa acgaccattc
     2041 tacctaccat tggtcaattt ggaggcgatg agtttgcgag agccaaagct atctatgagt
     2101 atttgaagag tcaaggggcg accaatcaag ccattgcggc tattttagga aactggtcag
     2161 tagaatcttc aatcaatccc aaacgagctg aaggtgacta cttatcgcct cctgttgggg
     2221 caacggatag ttcttgggac gatgagggat ggttgtctct aaatggtcca gctatctata
     2281 atggcaggta cccgaatatt ttaagacgtg gcttaggctt ggggcagtgg acagatacag
     2341 cagatgggtc acgcagacat accttattat tagagtatac caaacgaaaa aaccagaagt
     2401 ggtatgactt aggcttacaa ctggatttta tgctcaatgg ggacaatcct tactatacca
     2461 aatggttaaa agactttttc aataattcag gtagcccagc cagtcttgcc caagtctttc
     2521 tcatttattg ggagggaaat agtggtgata agctcctaga acgtcaaacg agagctacag
     2581 agtggtatta ccaaattgag aaaggcttta gccaaccaaa tggtggaacc gctcagagtg
     2641 atccaaaagc acttgaggct gttcgaggag atctttatga caactctatt ccaggtggag
     2701 gtgacggtat gggttatgcc tatgggcaat gtacgtgggg agtggccgct cgtatcaatc
     2761 aattgggctt gaaactcaaa ggacgaaatg gtgagaaaat cccaatcatt agtaccatgg
     2821 ggaatggtca agattgggtg gcaacagcag cacgtcttgg tggagataca ggtaagctac
     2881 caaaagcagg agcgattatt tcttttgcgg gaggaggtca tggatcgcca acagaatacg
     2941 g
//
LOCUS       DASFSW010000188         2904 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.189, whole genome shotgun sequence.
ACCESSION   DASFSW010000188 DASFSW010000000
VERSION     DASFSW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.189"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(72..1238)
                     /locus_tag="U1099_002023"
     CDS             complement(72..1238)
                     /locus_tag="U1099_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEM4868299.1"
                     /translation="MNYLVISPFYPENFQPFTIELAKKEGVTVLGIGQEPYDQLPEEL
                     RNALTEYFRVEDLENIDEVKRAAAFLIYKHGPIDRVESHNEYWLENDAALREQFNIFG
                     AKPKHLKKTKFKSEMKKYFKKAGVPVVPGVVVKSERDFGRALKNVGFPMIAKPDNGVG
                     ASGTFKIENKEDFDRFVETWDGATIYFLEKFVNSSIITTYDGLLDHEGNVVFETGLTY
                     VHTPLELMLSRKDNAYFIEKELDPQLVKYGRAIIKAFGMRERFFHIEFFKDGDDYIAI
                     EYNNRMAGGFTVEAYNYAHSIDLFRDYANVATGGTVEERRFEPQYCLVATRRDTTEYV
                     HSADDIHQKFAGKIKTVKRMPDAFAELQGNDAYLLVTESKKELDEMIAYIGLTK"
     gene            complement(1257..2003)
                     /locus_tag="U1099_002024"
     CDS             complement(1257..2003)
                     /locus_tag="U1099_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEM4868300.1"
                     /translation="MHVEFLSHWSGNLGREMNLNRYGHAGIPVVVFASSGGSYNEYAD
                     FGMIEACRGSIEAGQVQFFTLTSYDSESWLADWKSIHDKAEAHRAYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFNNNHDYGHDD
                     AVYQNSPSDYIWNQNDGWFIDKYRQADIIVCTGLGDWEQDGLDSFYNLKRAFEEKNIP
                     AWFDTWGTDVAHDWVWWRKQMPFFLHSIGL"
     gene            complement(2034..2861)
                     /locus_tag="U1099_002025"
     CDS             complement(2034..2861)
                     /locus_tag="U1099_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM4868301.1"
                     /translation="MNKSYFYLDMRTHELDVPFTGRKRRVRVLLPKGYAENTEETYPV
                     VYFHDGQNVLYSKEAFSGHSWKVIPTIKRNPDIAKMIVVAIDNDGADRMHEYAAWKFS
                     EMGIPGVQFGGKGTLYAEFVMDVVKPFIDKEYRTKSDKVHTAMIGSSLGGNITQFMGL
                     AYQNQIGCLGVFSSANWLHQDAFDRYIERQQLDSEQRVYIYVGTEEADDTDKTLMAGN
                     IKQAYINSSLTYYRQLIAGGVDLDNIALEVVSGAVHNEEAWALYLPACLRFLSEHWS"
BASE COUNT          850 a          651 c          540 g          863 t
ORIGIN      
        1 ttctgaacta aaaacttaca ggtttgtaag aaaagaaaaa agacaccatt ttaggcatct
       61 ttcaattcat tctattttgt taaaccaatg taggcaatca tttcatccaa ctctttcttg
      121 ctttccgtta caagtagata ggcatcattt ccctgaagtt ccgcaaaggc atctggcatg
      181 cgtttgacgg ttttaatctt accagcgaat ttctgatgaa tgtcatctgc tgagtgaaca
      241 tattcggtcg tatcacggcg agttgcgacc aagcagtatt gtggttcaaa tctacgttcc
      301 tcaactgtac cacctgttgc gacattggca taatcacgga aaagatcaat ggaatgggcg
      361 tagttatagg cttcaacagt aaagccaccc gccatgcggt tgttgtattc aatggcaatg
      421 tagtcgtccc catccttgaa aaattcaata tggaaaaaac gctctctcat accaaaggcc
      481 ttgataatcg cgcgaccata ttttactaat tgaggatcca actccttctc aatgaagtag
      541 gcattgtctt ttctagacaa catcagttcc agcggagtat gaacataagt caaacctgtt
      601 tcaaagacaa cattgccttc gtggtccaac aaaccatcat aggttgtgat aatgcttgag
      661 ttgacaaatt tttcgaggaa ataaatagtt gctccatccc atgtctccac aaaccgatca
      721 aaatcttctt tattttcgat tttaaaggta ccggatgccc caacaccgtt gtctggttta
      781 gcaatcattg ggaaaccaac attcttcaaa gctctgccaa aatcacgttc cgatttaaca
      841 accacacctg gaacgactgg aacacctgct tttttgaaat attttttcat ttcagactta
      901 aatttagtct ttttcaggtg tttgggtttg gctccaaaaa tgttgaattg ttcacggagg
      961 gctgcatcgt tttcaagcca atattcattg tgggattcta cacggtcaat tggaccatgc
     1021 ttgtaaataa gaaaggccgc tgcacgcttg acttcatcga tattttccaa atcttccaca
     1081 cgaaagtatt ctgtaagagc gttacgaagt tcttcaggca attgatcgta aggttcttgc
     1141 ccaataccta gaaccgtaac gccttctttt ttagccaatt caatggtgaa cggttgaaaa
     1201 ttttctggat aaaaaggtga aataacgaga taattcatag gtagtccttt ctaatcttaa
     1261 agtccgatag aatgaaggaa gaatggcatt tgctttctcc accacaccca gtcgtgggcc
     1321 acatctgttc cccaagtatc aaaccaagct gggatattct tctcttcaaa ggcacgtttt
     1381 aggttgtaga atgaatccag accgtcttgc tcccaatcgc ccaaacctgt gcagacgata
     1441 atatctgctt gacggtattt gtcaataaac caaccatcat tctggttcca gatgtaatca
     1501 gacggtgagt tttgatagac cgcatcatca tgaccataat catggttgtt gttgaagaat
     1561 cgagcatcat agacacctga aagtgcaata actttcgtga agacatctgg atgctggagg
     1621 aagaaattga gcgcgtggta ggcccccatt gagcaaccag ttgtcatcat accatcgaac
     1681 cagccagttt tatgtttgat aaagggaatc gcttcttcaa tcacataccg ttcataggcg
     1741 cggtgagcct ctgccttatc atgaatggat ttccagtctg ccaaccagct ttcactatcg
     1801 tagctagtta gagtaaaaaa ttggacctga ccagcttcaa tagatccacg acaagcttca
     1861 atcataccaa aatcagcata ttcattgtaa gaaccacctg atgaagcaaa tacgacaaca
     1921 ggaatccctg cgtgaccata acgatttaga ttcatttctc ggccaagatt tccagaccaa
     1981 tgacttaaaa attcaacgtg catagtaaag cctcctattt gtatattttt gttttatgac
     2041 caatgttcgc ttaggaatcg taggcaagct ggcaaataga gcgcccaagc ctcttcatta
     2101 tggaccgcac ccgaaacgac ttccaaggcg atattgtcca aatccactcc gccagcgatc
     2161 aattgacgat agtaagtcaa tgaagaattg atataggcct gcttgatatt tcctgccatc
     2221 aatgttttat ctgtatcatc tgcttcttct gtcccaacgt agatgtagac tcgttgctct
     2281 gagtctaatt gttgacgttc aatataacgg tcgaaagcat cttgatgaag ccaattggcc
     2341 gatgaaaaga cacccagaca gccaatctgg ttctgataag ccaagcccat aaattgtgtg
     2401 atattgcctc ccaaggatga gccaatcatt gcagtgtgaa ctttatcaga ttttgtccgg
     2461 tattctttat cgataaaggg tttgacgaca tccatcacaa attcagcata caaggttccc
     2521 ttaccaccga actgcacccc aggaatcccc atctcactaa acttccaagc agcgtattca
     2581 tgcatgcgat cagcaccatc gttatcaata gctactacga tcatttttgc aatgtctgga
     2641 ttacgcttaa tagttgggat gactttccat gaatgacctg aaaaagcttc cttactgtag
     2701 agtacatttt gcccatcgtg gaaataaaca acgggatagg tctcttctgt attttcagca
     2761 tatcctttcg gcaataaaac acggactcga cgttttcttc ctgtaaatgg tacatctaat
     2821 tcatgagttc tcatgtccaa ataaaagtaa gacttattca tatctatctc ttccttatgt
     2881 attttaagta ttatatcata gttt
//
LOCUS       DASFSW010000189         2903 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.190, whole genome shotgun sequence.
ACCESSION   DASFSW010000189 DASFSW010000000
VERSION     DASFSW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.190"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(13..207)
                     /locus_tag="U1099_002026"
     CDS             complement(13..207)
                     /locus_tag="U1099_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEM4868302.1"
                     /translation="MKVKLIPERCIACGLCQTYSSLFDYDDDGIVVFSDESQFEKTIE
                     PNPDILKAIKSCPTKAISAD"
     gene            258..734
                     /locus_tag="U1099_002027"
     CDS             258..734
                     /locus_tag="U1099_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM4868303.1"
                     /translation="MSQEPWNEEVYHTETTSRKERLSKGVADTTVFTVLAIIFFVIVL
                     IIGIMAVYLSIGGGSKTDSTKEFYTPSTTTVVAESSSSAAETQESTSTDTTETETSSS
                     PAEATDGSTLTVQAGEGVGQLAARGGISIAELERLNPEKMTTGSWIAHPGDVVRIR"
     gene            744..1421
                     /locus_tag="U1099_002028"
     CDS             744..1421
                     /locus_tag="U1099_002028"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEM4868304.1"
                     /translation="MKSIQIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYL
                     ALTNGIEVTDQNRIVDLLAQYPIRFGRDENGQQLVFVGDEDVTLPIRDNQVTNNVSAV
                     AALPLVREELVRLQQDIAQAGGIVMDGRDIGTVVLPQAELKIFLVASVEERALRRFKE
                     NTERGIETDLESLKEEIAARDYKDSNREVSPLKAADDAITFDTTGVSIEGVVKFISEK
                     AKEILDR"
     misc_feature    1448..1576
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1602..2123
                     /gene="infC"
                     /locus_tag="U1099_002029"
     CDS             1602..2123
                     /gene="infC"
                     /locus_tag="U1099_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEM4868305.1"
                     /translation="MAKQDLFINDEIRVREVRLIGLEGEQLGIKPLNEAQAIADSANV
                     DLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGDFET
                     KLRHARKFLEKGNKVKVSIRFKGRMITHKEVGAKVLAEFAEATQDIAIIEQRAKMDGR
                     QMFMQLAPASDKK"
     gene            2150..2350
                     /gene="rpmI"
                     /locus_tag="U1099_002030"
     CDS             2150..2350
                     /gene="rpmI"
                     /locus_tag="U1099_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEM4868306.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKAGMVHAGDFKRIKSMLTGLK"
     gene            2394..2753
                     /gene="rplT"
                     /locus_tag="U1099_002031"
     CDS             2394..2753
                     /gene="rplT"
                     /locus_tag="U1099_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEM4868307.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHLLFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLAK"
BASE COUNT          926 a          527 c          646 g          804 t
ORIGIN      
        1 aaaaaagcca aatcaatccg ctgaaatggc ttttgtagga catgatttga tggctttaag
       61 aatatctgga ttgggttcaa tcgttttctc aaattgtgat tcgtctgaaa aaacaacgat
      121 accgtcgtca tcataatcga atagagatga gtaggtttgg cagagcccgc aagcaatgca
      181 ccgttcagga ataagtttta ctttcatatt agtattgtaa tcatatttta ataaaaatac
      241 aaggagtttt ctcaataatg tcacaagagc catggaatga agaagtttac cacacagaaa
      301 caactagtcg taaagaacgt ctgagtaagg gggtagcgga tacaacagta tttacagttt
      361 tggcaattat tttctttgtt atcgtactga ttattggaat tatggctgtc tatctctcaa
      421 tcggtggcgg tagtaaaaca gattcaacaa aagaatttta taccccaagc actacaacag
      481 tcgtagcaga gagctcttca tcggctgctg agacacaaga atctacttcg actgacacga
      541 cagaaacaga aacaagttct agcccagcgg aagcaacgga tggctctacc cttactgttc
      601 aagcaggtga aggtgtagga caactggctg ctcgtggtgg gatttcaatt gcagagctag
      661 agcgtttgaa tccagagaaa atgactacag ggtcttggat tgcgcatcct ggggatgtag
      721 ttcgtattag ataaagagga gttatgaaat caattcaaat tgcaattgac ggtccagctt
      781 caagtggaaa gtcaacggtt gctaaaatta ttgctaaaaa tttgggatat acttacttag
      841 atacaggagc tatgtatcgc tcggctacct atctagcctt gacaaacggc atagaggtca
      901 cagatcaaaa tagaatcgtg gacttgcttg cccagtatcc cattcgattt gggcgtgatg
      961 aaaacggtca gcaacttgtt tttgtcggtg atgaggatgt tactttgcct attcgagata
     1021 atcaagtaac aaataatgta tcagcggtag cagcgcttcc tctagtacgg gaagaattgg
     1081 tccgcctgca acaggacatt gcgcaagctg gtgggattgt tatggatggt cgtgatattg
     1141 gtacggttgt attgccgcaa gcagaactca aaatattcct tgttgcctct gttgaagagc
     1201 gtgcccttcg tcgatttaag gaaaatacgg aacgtggcat tgagacagac ttggaatcct
     1261 tgaaagagga gattgcggct cgtgactata aagattccaa tcgagaagtt tctcctttaa
     1321 aggcagcaga tgatgctatt acctttgata caacaggagt atctatcgaa ggtgtagtga
     1381 aatttatttc cgaaaaagcg aaagaaattc ttgacaggta gctttagaca tgttagaata
     1441 gtcgtattga gaaaagcaga agtgagaact tctcgccttg cgactaacgt tgtctggctc
     1501 tacggattaa gatttcttcg gaaagaataa tggtgtagtg cggacttgtt tagcaagtcc
     1561 gtttcgtttt tctcgaaaaa tataaaatga ggtgaaaagc catagcaaag caagatttgt
     1621 tcatcaatga tgaaatccga gtgcgtgaag ttcgtcttat cggtcttgag ggtgaacagt
     1681 tgggaatcaa accactcaat gaggctcagg ccattgctga tagcgccaac gtggatttag
     1741 tattgattca accccaagct aaaccaccag tagcgaaaat tatggactac ggtaagttca
     1801 aatttgaata tcagaagaaa cagaaagaac aacgcaaaaa acaaagtgtt gtcactgtaa
     1861 aagaagtgcg tctaagtcca gttattgata aaggggattt cgagacaaaa cttcgtcatg
     1921 cccgtaaatt tcttgaaaaa ggaaataaag ttaaggtttc gatccgtttt aagggtcgta
     1981 tgattaccca caaagaagtt ggagcgaaag tattagctga gttcgctgaa gcaacccaag
     2041 atattgccat tatcgaacaa cgcgctaaga tggacggacg tcaaatgttt atgcaattgg
     2101 ccccagcttc agataaaaaa taagcttttt aagcaaaagg agaattaaca tgccaaaaca
     2161 aaaaactcac cgcgcatcag ctaaacgttt caaacgtaca ggttctggtg gattgaaacg
     2221 cttccgcgct tatacttcac accgtttcca cggtaaaact aaaaaacaac gtcgtcacct
     2281 tcgtaaagca ggtatggtac atgctggtga cttcaagcgt atcaaatcaa tgcttacagg
     2341 tttgaaataa gccgacttac tagaatttag attatcattt ggaggaaaat taaatggcac
     2401 gtgttaaagg tggcgttgtt tctcgtaaac gccgtaaacg tattttaaaa ttagctaaag
     2461 gttactacgg agctaaacat ctcttgttcc gcactgcgaa agaacaagtc atgaactctt
     2521 actactatgc ataccgtgac cgccgtcaga aaaaacgtga tttccgtaaa ttgtggatca
     2581 ctcgtatcaa tgcggctgct cgtatgaacg gtttgtcata ctcacaattg atgcacggtt
     2641 tgaaattggc cgaaatcgaa gtaaaccgca aaatgcttgc tgacttggca gtgaacgatg
     2701 cagcagcttt cacagctctt gcagatgctg ctaaagcaaa acttgctaaa taagtataaa
     2761 aatgtaaaga acttggtagt gatgaaccag gttctttatt tttggaaagt agctcactac
     2821 ttgctaaatt tcgatatgag tgatatactt aactagtaaa taaatcgacg gttttaactt
     2881 tatcgtcatt tagtttttct ttt
//
LOCUS       DASFSW010000190         2893 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.191, whole genome shotgun sequence.
ACCESSION   DASFSW010000190 DASFSW010000000
VERSION     DASFSW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.191"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            34..1161
                     /locus_tag="U1099_002032"
     CDS             34..1161
                     /locus_tag="U1099_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4868308.1"
                     /translation="MYRASVYLGIDAITGKKVKTNVTGRTKKEVKNKTQQAIATFKTD
                     GATRYQNATITSYKELAELWWNSYKHTVKPNTRGNIRGLLKNHVIPLFGAYKLDKLTT
                     PLLQSIVIKLADKANTGEAGAYLHYDKIHALNKRILQYGVVMQVLPYNPAREVILPRN
                     AKKATRKKVKHFNDEQLKQFLGYLDGLDLTNYRNLYEATLYKFLLATGCRINEVLALH
                     WSDIDLNNATVSITKTLNRYGSINSPKSNASIRDINIDSQTVAMMKEYRRRQIQEAWT
                     LGRSETVVFSDFIHDYPEDKTLGNRLTTRLRNIGLPNIGFHGFRHTHASLLLNSGIPY
                     KELQHRLGHSRISITMDIYSHLSKENAKNAVAFYEKALGNL"
     gene            1299..1682
                     /gene="yaaA"
                     /locus_tag="U1099_002033"
     CDS             1299..1682
                     /gene="yaaA"
                     /locus_tag="U1099_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEM4868309.1"
                     /translation="MEFKLFDDYITLQALLKTTGILHSGGAIKGFLEENTILFNGEDE
                     KRRGKKIRVGDVITLPDHNTSITIVAPSAEEIKQHQEDQAEKERVAAIVKKLNQENKK
                     NKKQVKTPKKATKNTERKPVRFPGM"
     gene            1685..2779
                     /gene="recF"
                     /locus_tag="U1099_002034"
     CDS             1685..2779
                     /gene="recF"
                     /locus_tag="U1099_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEM4868310.1"
                     /translation="MWLERLELQHFRNYNQLDIEFHKGLNVFLGENAQGKTNILESIY
                     VLALTRSHRTRTDKDLLQFQEKELSISGLLHRTSGKVPLDIHLTDKGRVTKVNHLKQA
                     KLSNYIGHMNVVFFAPEDLQLIKGAPALRRKFIDVELGQIKPLYLSDLSNYNHVLKQR
                     NTYLKSTDKIDENFLSVLDQQLAEYGSRVIQHRIDFLKKLEEFGNRKVQEISGNREEL
                     TIEYQTSIKLTDDVNLIDKFLTELERCRKRDLFKKNTGVGPHRDDVAFFINGMNAHYG
                     SQGQHRSLVLSLKLAEIELMKEVTREYPILLLDDVMSELDNNRQIKLLETITDTIQTF
                     ITTTSLDHLHKLPDSLKIFHIESGKVTESE"
BASE COUNT         1015 a          606 c          564 g          708 t
ORIGIN      
        1 ataactgaag taaaaaagaa aaacggtgct actgtgtacc gtgccagtgt ttatctagga
       61 atagatgcca tcactggtaa gaaagtcaag actaatgtca cgggtaggac caagaaggag
      121 gttaaaaaca aaactcagca agctattgca acttttaaaa cagacggggc aacacgctac
      181 caaaatgcca ccataaccag ttacaaagag ttggcagaat tgtggtggaa tagctataag
      241 cacacagtaa aaccaaatac ccgtggaaac atcaggggct tactgaaaaa tcatgttata
      301 ccactttttg gagcctataa gctcgataaa ctgacgaccc cactcctcca aagcatcgtc
      361 atcaaattag cagacaaagc aaatacaggg gaagctggtg cctatctgca ctatgacaaa
      421 atccacgcgc tgaacaaacg tatattgcaa tacggtgttg ttatgcaagt tctgccatac
      481 aatccagctc gggaagtcat attgcccagg aacgcaaaaa aagccactcg gaagaaggta
      541 aagcatttca acgacgagca gcttaaacag tttcttggct acttggacgg cttagactta
      601 accaattaca gaaatctgta tgaagccacc ctatacaagt tcttgctggc cactgggtgc
      661 cgtatcaatg aggtgctggc actgcactgg tcagatattg acttgaacaa cgccacggtc
      721 agcatcacga agacactaaa ccgctatggg tcaatcaatt cccccaagtc aaatgccagc
      781 atacgcgata taaacatcga tagccagaca gtagccatga tgaaagaata cagacggcga
      841 cagatacaag aggcttggac cctcggacgc tctgaaacgg ttgtattttc cgactttatc
      901 catgactatc ctgaggataa gaccctgggg aacaggttga ccacacgcct gaggaatatc
      961 ggactgccta acatcggctt tcacggtttc cgccacaccc acgctagtct actgcttaac
     1021 tctggaatac cctacaagga actccagcac cgccttggtc attctagaat atcaataacc
     1081 atggacatct atagccacct ctcaaaagag aatgcaaaaa acgctgttgc attttacgaa
     1141 aaagctctcg ggaatctttg agggtgaaca aaaaggtgaa caaattcagt ttacagtact
     1201 ttacaagact gtaaaacccc ttgatacaac tggggtttca gcccctaaaa tcgaaattat
     1261 accattttgt gatataatat tctgtaacga ggtgacttat ggaatttaaa ttatttgatg
     1321 actacatcac attacaagca ttgttaaaaa cgactggtat tctccattca ggaggtgcca
     1381 ttaaaggttt cttagaagaa aataccattc ttttcaatgg agaggatgaa aaaagacgcg
     1441 gtaaaaaaat ccgtgtcgga gatgttatca cactcccaga ccacaacacc agcatcacaa
     1501 tcgtcgctcc tagtgcagag gaaatcaaac agcaccaaga ggatcaagct gaaaaagaac
     1561 gtgtggctgc aatcgttaaa aagttaaatc aagaaaataa aaagaataag aaacaggtaa
     1621 aaacgcctaa aaaagcaact aaaaatactg aaagaaaacc tgttcgtttc cctggtatgt
     1681 agacatgtgg ctagaacgat tagagttaca acattttcgc aattacaacc aattagatat
     1741 tgagttccac aaagggctca atgtcttttt aggcgaaaac gcccaaggaa aaaccaatat
     1801 tctagaatct atctatgttt tagccttaac acgaagtcat cgaacacgga cagataagga
     1861 cctactacag tttcaagaaa aggaattatc tatttctggt ttactccatc gcacaagcgg
     1921 taaagttccc cttgacattc acttgacaga caaggggcga gtgaccaagg tcaaccatct
     1981 caaacaagcc aagctttcca actatatcgg ccacatgaat gttgttttct tcgcccctga
     2041 agatttacag ctgataaaag gtgcgcctgc tttgagaaga aagttcatcg atgtcgagct
     2101 cgggcaaatc aagccactct atctctctga cctatcgaat tataaccacg ttttaaaaca
     2161 aagaaatact tacctcaaat ctacagataa gattgatgag aacttcctat ccgtgttaga
     2221 tcagcaactt gctgaatatg gtagtcgtgt tatccaacat cgtattgact ttctaaagaa
     2281 attagaggaa ttcggaaata gaaaagttca agaaatatct ggcaatcggg aagaattgac
     2341 aatcgaatac caaacctcaa tcaaattgac agatgatgtc aatttaattg acaaattctt
     2401 gacggaattg gaaaggtgtc gcaaacgtga tttatttaag aaaaatacag gagttggacc
     2461 acatcgagat gatgtagctt tctttataaa tggaatgaat gcccattatg gtagccaagg
     2521 tcagcaccgc agtctcgttc tgtcactcaa actggctgaa attgagctga tgaaggaggt
     2581 cacacgagaa tacccaattc tactcttaga tgatgttatg agtgagctag ataataatcg
     2641 tcaaatcaaa ctactagaaa ctattacaga tacgattcaa acatttatta caacgacatc
     2701 tttagaccat ttacataagc tacctgatag tttaaaaatc ttccatattg agtctggtaa
     2761 ggttactgag tcagaataag attgtttaca ctgttgtcaa taaagtttac aaaaaagtaa
     2821 aaccctgcca agagggtctt gcagcacgta gacaaactcc taaaaatata aaaaattgaa
     2881 gaagtttgcg aaa
//
LOCUS       DASFSW010000191         2875 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.192, whole genome shotgun sequence.
ACCESSION   DASFSW010000191 DASFSW010000000
VERSION     DASFSW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.192"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            16..807
                     /locus_tag="U1099_002035"
     CDS             16..807
                     /locus_tag="U1099_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM4868311.1"
                     /translation="MKNEHGEQAVVMGLGITFQKKKGDLIVKDKIENIFSLKNDEAKE
                     NFLTLLKDIPLDFISATYTVINHLVETYHYPVQEYLYVTLTDHIYCAYQAVLKNTYQE
                     SRLPNISAEYPLEYKMAREALAMFREKLLNDFPNDEIGRIALHLINAKGETIHPTSEE
                     HDISKKIIAEVEQILIENGIKRSKENSNFYDRFMIHLSYFLNYLDRSHQSNESIASLE
                     QYIKLQNPRAHQVGSQIFEMITSLVDEPVNDSERFYIVLHIQRLL"
     gene            839..1156
                     /locus_tag="U1099_002036"
     CDS             839..1156
                     /locus_tag="U1099_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM4868312.1"
                     /translation="MNREEITLLGFEIVAFAGDARSRLMEALAAAQKGDYAKAEELVE
                     AANACIVEAHHAQTSLLQKEAAGEDLAFSVTLMHGQDHLMTTLLLKDMMGHMIELYKR
                     GDK"
     gene            1156..2835
                     /locus_tag="U1099_002037"
     CDS             1156..2835
                     /locus_tag="U1099_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEM4868313.1"
                     /translation="MNKLIEFIEKGKPFFEKISRNPYLRAIRDGFIAAMPVILFSTLF
                     LMVAFVPNIFGFTWSDEAVAAIMKPYGYTMGIVAVLVAGTTAKSLTDAFNRQLPKTNQ
                     INFISTMIASISGFLLLASDGIEGGFANGYMGTKGLLTAFLAAFITVNIYKVCVKNNV
                     TIRMPDEVPPNVSQAFKNVIPYALSIFVLYGIDLVTRQFLGTNVAEAILKLFEPLFTA
                     ADGYVGITIIFGAYALFWFVGIHGPSIVEPAIAAITYANIETNFQLLQAGQHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPLV
                     LNPVFFVPFILAPIANVWIFKFFVDTLKMNSFSVNLPWTTPGPLGIVMGTNFAPLAFA
                     LAILLVVVDVLIYYPFLKVYDEQILAEEQSGKVENSLKEKVAANFNTAKADAILEKAA
                     VETEISEQTNVLVLCAGGGTSGLLANALTKAAKEYGVPVTATAGSYGAHREILPEYQL
                     VILAPQVASNYDDMKQETDALGIKLAKTEGAQYIKLTRDGQGALDFVKQQF"
BASE COUNT          871 a          513 c          599 g          892 t
ORIGIN      
        1 aataacgctg ccctggtgaa aaatgaacat ggggaacagg cagtcgtgat gggattaggt
       61 ataacctttc aaaagaagaa aggggattta attgttaagg acaagataga aaatatcttt
      121 tcgctaaaaa atgatgaagc caaagaaaat tttctaacct tattaaaaga tattccctta
      181 gattttattt cggcaaccta cacggttatt aatcatctgg tagaaactta tcattaccct
      241 gtacaagaat atctatatgt gacactgaca gatcatatct attgtgcata tcaggcggtt
      301 ttaaagaata cctatcaaga aagtaggtta cctaatattt cagccgaata tccattggaa
      361 tataaaatgg cgcgtgaagc cttagctatg tttcgtgaaa agttgctaaa tgattttcca
      421 aatgatgaga ttggtagaat tgcacttcac ttaatcaatg caaagggtga aaccattcat
      481 cccactagtg aagaacatga catcagtaag aaaattattg ctgaggttga acaaattttg
      541 atagagaatg ggattaaacg ttcaaaagaa aatagcaatt tttatgatcg ctttatgatt
      601 catttgtcct attttctgaa ttacttagat agatcccatc agtcaaatga atcgattgct
      661 agtttggaac agtacatcaa gctccagaat ccaagagccc atcaggttgg aagtcagatt
      721 tttgagatga ttacatcatt ggttgatgag ccagtaaatg atagtgaaag gttctatatt
      781 gtactacata ttcaacggtt attgtaaatc aattcaattt taaaaggagg ttcatattat
      841 gaacagagaa gaaattactt tattaggttt tgagattgtt gcattcgctg gtgatgctcg
      901 ctctcgtttg atggaggcac tggctgctgc tcaaaaaggt gattacgcaa aagcagaaga
      961 actagttgaa gctgccaatg cttgtattgt tgaagcccac catgctcaga caagtttgtt
     1021 gcagaaagaa gcggctggtg aggatttagc ctttagtgtg acgctcatgc atggtcaaga
     1081 ccatctcatg acgacattat tattgaaaga tatgatgggt catatgattg aactttacaa
     1141 acgaggtgat aagtaatgaa taaattgatt gaattcattg agaaagggaa gcctttcttt
     1201 gaaaagattt cgagaaaccc ttatttaagg gctatccgtg atggttttat cgctgccatg
     1261 ccggttatct tgttctcaac tctctttcta atggtagcat ttgtacctaa tatctttgga
     1321 tttacttggt ctgatgaagc agttgcggct atcatgaaac cttacggtta tacaatgggt
     1381 attgtagctg tcttagttgc aggaacgacc gcaaaatctt tgacagatgc gtttaaccgt
     1441 caattgccaa aaaccaatca gattaacttc atttcaacca tgattgcctc aatttcaggt
     1501 ttcttattac tggcttctga tggtattgaa ggtggatttg ccaatggtta catgggaact
     1561 aagggacttt tgacagcctt tctggcagcc ttcattacgg ttaatatcta taaggtctgt
     1621 gtgaaaaaca atgttaccat tcgtatgcca gacgaagttc caccgaacgt atcccaggca
     1681 tttaaaaatg tgattccata tgcattgtct atctttgtct tgtatggaat tgatcttgtg
     1741 acacgtcaat tccttggaac aaacgttgct gaggcgatcc tgaaattatt tgagcctcta
     1801 tttacagccg cagatggtta tgttggtatt acaattatct ttggtgccta tgctttgttc
     1861 tggtttgtag ggattcatgg tccatctatt gtggaaccag caattgcagc cattacttat
     1921 gccaatattg aaaccaactt ccagcttcta caggcgggtc aacatgcgga caaaatcctg
     1981 acttcaggta ctcaaatgtt tatcgtaaca atgggtggta caggtgctac cttggttgtg
     2041 ccattcatgt ttatgtggtt gactaagtct aaacgaaata aagcaattgg acgtgcttca
     2101 gttgttccaa ctttctttgg tgtaaatgaa ccgatcttgt tcggtgcacc acttgtactc
     2161 aacccagtat tctttgttcc attcatctta gctccaattg ctaacgtatg gattttcaaa
     2221 ttctttgttg atacgctaaa aatgaacagt ttcagcgtta acttgccatg gacaactcca
     2281 ggaccattgg ggattgtaat ggggacaaac tttgctccac ttgcctttgc actagctatc
     2341 ttattggtag ttgtcgatgt acttatctat tatccattct tgaaagttta cgatgagcag
     2401 attctcgctg aagaacaatc agggaaagtt gaaaatagtt tgaaagagaa agtggcggct
     2461 aactttaata ctgccaaagc ggatgctatt cttgaaaaag ctgcagtcga aaccgaaatt
     2521 tctgaacaaa caaatgtttt ggtactttgt gcaggaggtg ggacaagcgg cttgcttgcc
     2581 aatgctttaa caaaagctgc aaaagaatac ggtgttccag tcacagcaac agcaggaagc
     2641 tatggggcgc atcgtgagat tttgccagag tatcaattag tcattctagc accacaagtg
     2701 gcatcaaact acgatgatat gaaacaagaa accgatgcat tgggtattaa acttgccaaa
     2761 actgaagggg ctcaatacat taaactcact cgtgatggtc aaggtgctct tgactttgtc
     2821 aaacaacagt tttagaacct atattcacag ttcagcactt ccatctaagg ctagt
//
LOCUS       DASFSW010000192         2822 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.193, whole genome shotgun sequence.
ACCESSION   DASFSW010000192 DASFSW010000000
VERSION     DASFSW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.193"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..855
                     /locus_tag="U1099_002038"
     CDS             <1..855
                     /locus_tag="U1099_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868314.1"
                     /translation="SIDIAPDSTTFLNVLDLSDDNMDEDPIKVKSEFLLSWIGKLLDR
                     KMDGREKSLIDRVTRLTYKHFATPSLVEWVFVLSKQPEQEAKDLALDMELYVEGSLDI
                     FSHRTNIKTDSHFLIYNVKKLGDELKQIALMVIFDQIWNRVVKNQKLGKKTWIYFDEM
                     QLLLLDKYASDFFFKLWSRVRKYGAIPTGITQNVETLLLDANGRRIIANSEFMILLKQ
                     AKSDREELVHLLGLSKELEKYLVNPEKGSGLIKAGSTVVPFRNKIPLQTQLFDIMSTD
                     PEKMRGES"
     gene            852..>2822
                     /locus_tag="U1099_002039"
     CDS             852..>2822
                     /locus_tag="U1099_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEM4868315.1"
                     /translation="MTREQQKVKVARKTFQSSLKASRIHYRREKKGLKRSLPKRRFIM
                     RRAEKAETREQRQTLKQTYQEEKDLATDTFKEAIAYVSPRWLKRKEIKKYRLPQARQR
                     LVVARKHLAEVKKAEKEEAVTSKFTDQKKPTESKTSRFQFRKEKPLERLQAEKEVKSA
                     KRDVKQLKKAHQFKNPSTKVKRGLRYVASESLDVVAQDDDLEGIRTLKESAIKGRRYG
                     RFTYQSGKLLVKSGQTGLRFTKTKVAHSRERYQNFKKGKGVTRQKPLKPRRRYQTFLK
                     QARKQSVSGITKVIQAIKNSLTFFSSIALNPMTWVVSGLLFFLLLMMSFVIGISGTTL
                     IQQDESELTKAYTHMTWEDAENTRTNPTGITYYTKIDDVMGFMNLKYQDYALEEIMEE
                     GDKTYHAYLSQLWQDLNGGDSLKSMLELTKEPAYKLSDEDREDLKELSEEGTYLALQE
                     LDNPFQGQTEDDALTMTVRYGYEVIDDKPTLHHHIILEAKENQVIVAPMDGKVSLDGE
                     NIIITSGKGLNKSQLTLFNIHTGRVSDGQKVQAGEVIGQTKDGAGLKMTYQKVDDDSE
                     KLVYVNPAFYFPKVIQLQTTILPTIGQFGGDEFARAKAIYEYLKSQGATNQAIAAILG
                     NWSVESSINPKRAEGDYLSPPVGATDSSWDDEG"
BASE COUNT          914 a          572 c          637 g          699 t
ORIGIN      
        1 agcattgata ttgcgcctga ctctacgact ttcttaaacg ttttagactt atccgatgac
       61 aatatggatg aagacccaat taaggtcaaa tctgaatttc tactttcttg gattgggaaa
      121 ctcttagacc ggaaaatgga tggtcgagaa aagtccctaa ttgaccgtgt gacacggtta
      181 acctataagc attttgccac accatctctc gtggaatggg tctttgttct ctctaaacaa
      241 cctgaacaag aagccaaaga cttagccctt gatatggagc tttatgtgga agggtcactc
      301 gatattttct cacaccgaac caacattaaa actgacagcc atttcctcat ttacaatgtc
      361 aaaaaattag gagatgaatt aaagcagatt gccctcatgg tcatttttga ccaaatttgg
      421 aaccgtgtgg ttaaaaacca aaaacttggc aagaagacct ggatttactt tgatgaaatg
      481 cagcttctgt tgttggataa gtacgcttct gatttcttct ttaaattatg gagtcgtgtc
      541 cgtaagtatg gggccattcc aacaggcatt acccagaacg tggaaactct tttattagat
      601 gctaatggca gacgcattat cgctaatagc gagttcatga tacttctaaa acaagctaaa
      661 agtgaccgag aagagttggt acacctctta gggttatcta aagagcttga gaaatatttg
      721 gttaacccag aaaagggttc aggattaatt aaggcaggct caacggttgt tccctttagg
      781 aataaaatcc cacttcaaac ccaactcttt gacatcatga gcaccgatcc agaaaagatg
      841 aggggtgaat catgacaaga gaacaacaga aggtcaaggt agcccgtaaa acctttcaga
      901 gtagtttaaa ggctagtcgt atccattacc gtagggagaa gaaggggtta aaacgctcac
      961 ttcctaaacg acgctttatc atgcgccgag ctgagaaagc tgaaacaagg gaacaacgtc
     1021 agactctgaa gcaaacctat caggaggaga aggacctagc aacagatacc tttaaggaag
     1081 cgattgccta tgtgtcaccc agatggctaa agcgtaagga aattaagaag tatcggcttc
     1141 ctcaagccag acagcgccta gtagtagctc gaaaacactt ggcagaagtc aagaaggcag
     1201 aaaaagagga ggctgttacc tctaagttta ccgatcagaa aaagccaaca gaatcaaaaa
     1261 cgtcacggtt tcagtttcga aaagaaaaac ctcttgaacg ccttcaggca gaaaaagagg
     1321 tgaaatcagc taagcgtgac gtgaaacaac tgaaaaaagc acatcagttt aagaacccct
     1381 cgactaaggt caaacgaggg ttacgctatg tcgcttcaga atcacttgat gtagtcgctc
     1441 aagatgatga cttagaggga atcagaaccc taaaagagtc tgcgattaaa ggaagacgct
     1501 atgggagatt tacttatcag tcaggaaaac tccttgtcaa aagtggtcag acaggtcttc
     1561 ggtttactaa gacgaaagtc gctcatagta gggagcggta tcagaacttt aaaaagggga
     1621 aaggggtcac ccgacagaag cccttaaaac caagaagacg ttaccagacc tttctcaaac
     1681 aagctagaaa acagagtgtg tcaggaataa caaaggttat tcaagcgatt aagaatagcc
     1741 tgaccttctt ttcatcgatt gcccttaatc ctatgacttg ggtcgtgtca ggcctcttgt
     1801 ttttcttgct tttgatgatg agttttgtga taggaatttc aggaactact ctcattcaac
     1861 aggatgagag tgaactgaca aaagcctata cgcacatgac gtgggaagat gcggaaaaca
     1921 cacgaaccaa ccccactggc attacctact acaccaagat tgatgacgtt atgggcttta
     1981 tgaatctcaa gtatcaagat tatgctttag aagaaatcat ggaagaaggg gataagacct
     2041 atcacgccta tctcagtcaa ctttggcagg acttaaacgg tggggactct ttaaaatcca
     2101 tgcttgagtt gaccaaggag cctgcctata agctttctga tgaggataga gaagacttaa
     2161 aggaattaag tgaggaagga acctaccttg ccctccaaga attggataat ccctttcagg
     2221 gacagactga agacgatgcg ctaaccatga cggtccgcta tggctatgag gtgattgatg
     2281 acaagccaac gcttcatcac catatcattc tagaagctaa agaaaatcaa gtgattgtgg
     2341 ctcccatgga tggtaaggtt tccctagatg gcgagaacat tatcatcacg tcaggtaagg
     2401 gactaaacaa gtctcaattg accttgttta atatccatac tggtagagtg agcgatggcc
     2461 aaaaagtcca agcaggggaa gtgattggtc aaactaagga tggtgctggt ctaaaaatga
     2521 cctatcaaaa ggttgacgat gattcagaga aactggttta cgtcaatcca gccttttact
     2581 ttcctaaagt cattcaactc caaacgacca ttctcccgac tattggtcaa tttggaggcg
     2641 atgagtttgc gagagccaaa gctatctatg agtatttgaa aagccaagga gcgaccaatc
     2701 aagccattgc ggcaatttta ggaaactggt cagtagaatc ctctatcaat cccaaacgag
     2761 ctgaaggcga ctacttatcg cctcctgttg gggcaacgga tagttcttgg gacgatgagg
     2821 gc
//
LOCUS       DASFSW010000193         2808 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.194, whole genome shotgun sequence.
ACCESSION   DASFSW010000193 DASFSW010000000
VERSION     DASFSW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.194"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            135..1508
                     /gene="dnaA"
                     /locus_tag="U1099_002040"
     CDS             135..1508
                     /gene="dnaA"
                     /locus_tag="U1099_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEM4868316.1"
                     /translation="MNQEQLFWQRFIELAKVNFKPSIYDFYVADAKLLGINQQVANIF
                     LNRPFKKDFWEKNFEELMIAASFEIYGEPLAIQYQFTEDEQEIRNTTNTRSSIVHQVQ
                     TLEPATPQETFKPVHSGIKSQYTFANFVQGDNNHWAKAAALAVSDNLGELYNPLFIFG
                     GPGLGKTHILNAIGNKVLADNPQARIKYVSSETFINEFLEHLRLNDMESFKKTYRNLD
                     LLLIDDIQSLRNKATTQEEFFHTFNALHEKNKQIVLTSDRNPDHLDNLEERLVTRFKW
                     GLTSEITPPDFETRIAILRNKCENLPYNFTNETLSYLAGQFDSNVRDLEGALKDIHLI
                     ATMRQLSEISVEVAAEAIRSRKQTNPQNMVIPIEKIQTEVGNFYGVSLKELKGSKRVQ
                     HIVHARQVAMFLAREMTDNSLPKIGKEFGNRDHTTVMHAYNKIKTLLLDDENLEIEIT
                     SIKNKLR"
     gene            1662..2798
                     /gene="dnaN"
                     /locus_tag="U1099_002041"
     CDS             1662..2798
                     /gene="dnaN"
                     /locus_tag="U1099_002041"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEM4868317.1"
                     /translation="MIQFSINKNIFLQALSITKRAISTKNAIPILSTVKITVTSEGIT
                     LTGSNGQISIEHFISIQDENAGLLISSPGSILLEAGFFINVVSSMPDLVLDFNEIEQK
                     QIVLTSGKSEITLKGKEAEQYPRLQEVPTSKPLVLETKVLKQTINETAFAASTQESRP
                     ILTGVHFVLTENKNLKTVATDSHRMSQRKLVLDTSGDDFNVVIPSRSLREFTAVFTDD
                     IETVEVFFSNNQILFRSEHISFYTRLLEGTYPDTDRLIPTEFKTTAIFDTANLRHSME
                     RARLLSNATQNGTVKLEIANNVVSAHVNSPEVGRVNEELDTVEVSGEDLVISFNPTYL
                     IEALKATTSEQVKISFISSVRPFTLIPNNEGEDFIQLVTPVRTN"
BASE COUNT          958 a          515 c          479 g          856 t
ORIGIN      
        1 gcaaaggttc tgacttttat tcacatcttg tggaaaactt ttcttaacag tgtggatttt
       61 taaaattatc tgtggaaaac ttttgttttt tatggtacac tattctaacg aatataatgt
      121 gaaaggggga aaatatgaac caagaacaac ttttttggca acgatttatt gaattggcaa
      181 aggtaaattt taagccatct atttatgatt tttatgtcgc tgatgcaaaa ttactcggaa
      241 tcaaccagca agttgccaat attttcttaa atcgtccatt taaaaaagat ttctgggaaa
      301 aaaacttcga agagttaatg attgccgcta gttttgaaat ctacggagag cctcttgcca
      361 tccaatatca atttacagag gatgaacagg agattaggaa tactacaaac acaagaagtt
      421 caatagttca ccaggtacag acacttgagc cggctactcc tcaagaaact tttaaaccgg
      481 ttcattctgg tataaaatcc cagtacacct ttgctaattt tgtacaagga gacaataatc
      541 actgggcaaa ggctgcagct ttagctgtat ctgataacct aggtgagctc tacaatccat
      601 tattcatttt tggtggtcct ggtcttggaa aaactcatat tttaaatgcg attggaaata
      661 aggttctagc cgataatccc caggcaagga taaaatatgt ctcatcggaa acattcatca
      721 atgaattttt agaacacctc cgtctcaatg atatggaaag tttcaaaaaa acctatcgca
      781 atctggactt acttctaatt gatgacattc agtctctccg taataaagca acaacacagg
      841 aagaattttt ccatactttt aatgcgcttc atgaaaaaaa taagcagatt gtactcacaa
      901 gcgaccgtaa tcccgatcac ttagacaatt tggaagaaag actagtaaca cgtttcaaat
      961 gggggttaac cagtgaaatc actccacctg attttgaaac acgtatcgca attttacgta
     1021 acaagtgcga gaacctgcct tacaacttta caaatgagac gctatcctat ctagctgggc
     1081 aatttgattc gaacgtacgt gaccttgaag gtgccttaaa agatatccat ttgatagcca
     1141 ctatgcgtca actgtctgag ataagtgtcg aggttgctgc tgaggctatt cgatcaagaa
     1201 aacaaacaaa tccacaaaat atggttattc ctattgagaa aatccaaacc gaagtgggaa
     1261 atttctacgg tgtcagcttg aaagaattaa aaggttctaa gcgtgttcaa catatcgttc
     1321 acgcgcgaca agttgctatg tttttagcac gtgaaatgac agacaattcc cttccaaaaa
     1381 ttgggaaaga atttggtaat cgagaccata caaccgttat gcatgcatac aataaaataa
     1441 aaactctcct cttggatgat gagaatttag aaatagagat taccagtata aaaaataaac
     1501 ttcgttaacc tgtgtataac ttttttaaaa aactctgttt tttccacaag ttgtgaacaa
     1561 gttaatttcc gcagttttat tggtctttca tcacttttcc acagaataca cagagactac
     1621 tattactatt aaccttatag ataataaata aaggagaatc catgattcaa ttttctatta
     1681 ataaaaatat atttctacaa gcacttagta ttactaaacg ggcaatcagt accaaaaatg
     1741 ctattccaat tctttcaaca gtaaaaatta cagtaactag tgaaggaatc actttaactg
     1801 gttcaaatgg acaaatctcg attgaacatt ttatttctat tcaagatgaa aatgcaggac
     1861 ttttgatcag ttctccaggc tccattctct tagaagctgg tttctttatt aatgtcgtat
     1921 ccagcatgcc ggatttagtc cttgacttca atgaaattga acaaaagcaa atcgttttga
     1981 caagtggtaa gtctgaaatc acattaaagg gaaaagaagc agaacagtat cctcgtttac
     2041 aggaagttcc aacttcaaaa ccattggtgt tagaaaccaa agtattaaaa caaacaatta
     2101 atgaaacagc atttgcagct tctacacaag aaagtcgtcc tattcttacg ggtgttcatt
     2161 ttgttttaac agaaaataaa aatctaaaaa ctgttgcaac agattcacac cgtatgagcc
     2221 aacggaaatt ggtccttgat acctctggtg atgattttaa tgttgtcatt ccaagtcgtt
     2281 ctctccgtga atttactgca gtttttacag atgatattga aacagtagaa gtcttctttt
     2341 caaataatca aatccttttt agaagcgagc atattagctt ctatacacgc ttattagaag
     2401 gtacctaccc tgataccgac cgcttaattc caactgagtt taaaacaact gcaatttttg
     2461 atactgcaaa tcttcgtcac tcgatggagc gtgctcgtct tctttcaaat gcaacccaaa
     2521 atggtacagt aaaactagaa attgctaata atgttgtatc ggctcatgta aattctccag
     2581 aagttggacg tgtgaatgag gaattagata ctgtagaagt atcaggtgaa gatttagtaa
     2641 tcagctttaa cccaacttac ttgatagaag cattgaaagc cacaactagt gaacaagtga
     2701 aaattagctt tatctcttct gtccgtccat ttacattgat tccaaataat gaaggggaag
     2761 attttattca attggttaca ccagttcgta ccaactaaat agtattaa
//
LOCUS       DASFSW010000194         2808 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.195, whole genome shotgun sequence.
ACCESSION   DASFSW010000194 DASFSW010000000
VERSION     DASFSW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.195"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(42..668)
                     /locus_tag="U1099_002042"
     CDS             complement(42..668)
                     /locus_tag="U1099_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEM4868318.1"
                     /translation="MELVYMDGRKEPYTLSSIVAECANISHHAVQEHIRKQKDRLERF
                     GKVSFQMRPLPSGQQAKDYILNEQQATLLITFLKNTEQVANFKENLVKAFFEMRDELA
                     QFRYQRALEKPKRKALHEAIETWQEAPKHAHSTVTNLLLKGTTGMNKRQLMAHRGGHN
                     GIDSLTSQELIRYQALEDMAIAMINLGMTYQDIKNMVFRPLKNAPQGA"
     gene            complement(681..1427)
                     /locus_tag="U1099_002043"
     CDS             complement(681..1427)
                     /locus_tag="U1099_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEM4868319.1"
                     /translation="MRRNYSKVIEEMRTIHGLNLVAIGQRIGTDPRTVGKWAQGKHQP
                     NKDSRKKINNLYREVKQSMTTQTTIEPFEDFYDWSKMKKKKPTTEVIATQQFGNKSFE
                     IYGNKDNPLFIAVEVAEMIEVQNTTDLLKRIDEDEKLTYVISRAGQKREVNMLTEFGL
                     YEVLFQSRKPKAKEFKKVVKNILKEIRVNGYYMQGELIQDQPTQSAIELNSDMAYIKN
                     RLAELQSMTTMADIKIGLAKTYRIAELMED"
     gene            complement(1446..1673)
                     /locus_tag="U1099_002044"
     CDS             complement(1446..1673)
                     /locus_tag="U1099_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4868320.1"
                     /translation="MTQWTLKACRVNAGYTLRQVAKKVGKNFQTISKYEKDSTIIPFE
                     LLKDLSKLYRVKLDDIFLGDSTKKIELTPEE"
     gene            1827..2540
                     /locus_tag="U1099_002045"
     CDS             1827..2540
                     /locus_tag="U1099_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM4868321.1"
                     /translation="MAKNSPQDIKNREYFSANLNRIMKEKGVRQIDIHNDLDIPKSTI
                     TGYVKGRSLPTSGNLQKLADYLNVKKSDLDSRFSNNDVYKEFLLMLEKQLEEKKYLVN
                     SVSKEKIFQKVKERERRDKELIERVKGMNRSVKPEEFLSTITESLEEYNPKNDYGLLM
                     DLALKTSSILFWLDIVFQKRTSEFYIVFEDKNFNENYYTHFYEKINVLFEEIKAAALE
                     EARKDKNDFEDYKLTWGLD"
BASE COUNT          765 a          536 c          522 g          985 t
ORIGIN      
        1 ggctttgctg attggtctcg gtaagccttt ttttgttaat ctcacgcgcc ctgtggtgcg
       61 ttttttagtg gtctgaatac catattttta atatcttggt atgtcatgcc taagttgatc
      121 atagcaatag ccatgtcttc taatgcttgg tatctgataa gctcttggct ggttaggctg
      181 tcaataccat tatgtccgcc acggtgtgcc attagctggc gtttgttcat tccagtagtt
      241 ccctttagca aaaggtttgt aaccgtgctg tgcgcgtgtt ttggggcttc ctgccatgtt
      301 tcaatagctt catgtagtgc cttacgctta ggcttctcta gtgccctctg atatcggaat
      361 tgtgccagct cgtcccgcat ttcaaagaat gctttgacta ggttctcctt aaagtttgca
      421 acttgctcgg tattctttag aaaagtgatt agcaaagttg cctgttgctc gtttaagata
      481 taatcttttg cctgttgccc actcggtaaa ggtcgcattt gaaatgagac ctttccaaat
      541 cgttcaagcc tatctttctg ctttctgata tgttcctgta ctgcgtgatg gctgatgttg
      601 gcgcattctg ctacaatact gctcaatgta tacggctctt tccgtccgtc catgtaaact
      661 aattccatgc ttgtcccttt ctagtcttcc ataagttcag caattcggta tgttttggcc
      721 agtccaattt taatatctgc catggtggtc atgctttgca actctgcaag tcggttcttg
      781 atgtaagcca tgtcagaatt tagctcgata gctgactggg ttggttggtc ttgaattagt
      841 tcaccttgca tgtagtagcc gtttacacga atttctttca agatgttttt gactactttt
      901 ttaaactctt tggcttttgg tttgcgtgat tgaaaaagta cttcataaag tccaaactca
      961 gttaacatgt ttacttctcg tttttgacct gccctagata ttacataggt cagcttctca
     1021 tcttcatcaa tcctttttaa taagtcggtt gtattttgta cttcaatcat ttcagccact
     1081 tcaacagcaa taaaaagtgg gttgtcttta tttccataaa tttcaaatga cttatttccg
     1141 aattgttgag tagcgataac ctcggtagtc ggtttctttt tcttcatttt tgaccagtcg
     1201 taaaaatctt cgaatggttc aattgtggtt tgtgttgtca tactctgttt tacctctctg
     1261 tatagattat tgattttctt tctgctgtcc ttgttgggtt ggtgtttgcc ctgtgcccac
     1321 ttgccaactg ttcgggggtc tgttccgata cgctgaccaa tagcaaccaa gttcagcccg
     1381 tgaatggttc gcatttcttc aatgacttta ctatagttcc ttctcattgc ttgcctgctc
     1441 ctttcttatt cctctggcgt tagttctatt tttttcgtac tgtcgcctaa aaaaatatca
     1501 tctaacttaa cacgatacaa cttggacaag tctttcaaca actcaaaagg gattatagta
     1561 ctatcttttt cgtactttga gatagtttga aaattcttgc ctactttttt agctacctgt
     1621 cgcaaggtat aacctgcatt tactcgacac gccttcagcg tccattgcgt catactcttt
     1681 tagctccttc caaaagtgat aaaacaatag tactattatt ttcgtactta gtcaagtgtt
     1741 tttttacaaa agttttattt ttttcgtacc ttgtcatttt ttatgttata atcaaaatag
     1801 ataaatgttg aaacggaggg gattacatgg caaagaatag cccacaggat ataaaaaata
     1861 gagaatattt ttctgctaac ctcaatagaa ttatgaaaga aaaaggagtt cggcaaatag
     1921 atattcataa tgatctagat ataccaaaaa gtacaatcac agggtacgtt aaaggacgtt
     1981 cgttgccaac gtcaggaaat ttacaaaaac ttgctgatta tctcaatgta aaaaagtcgg
     2041 accttgattc tcgcttctca aacaatgatg tctataaaga atttctgtta atgcttgaaa
     2101 agcaattgga agaaaaaaaa taccttgtaa atagcgtgtc aaaagaaaaa atttttcaaa
     2161 aggtaaaaga aagagaacgt agagacaaag aattaattga acgcgttaaa ggtatgaatc
     2221 gctctgtgaa acctgaagag tttcttagca ctataactga atcgttggaa gaatataacc
     2281 ccaaaaatga ctatggttta cttatggacc ttgctttgaa aacgagtagc atactttttt
     2341 ggcttgatat agtatttcaa aaaagaacga gtgagtttta tatagttttt gaagataaaa
     2401 acttcaacga aaattactat actcattttt atgaaaaaat taatgtttta tttgaagaga
     2461 taaaggctgc tgctttagaa gaagctagaa aagataaaaa tgattttgaa gattataagt
     2521 taacgtgggg attagactaa ccgcccacgc tccctacacg ccccctcacc acgttcaaac
     2581 acttctcggg tatatttacc ctgccaagct attccacgcg cccaggggct ttctagtgga
     2641 ctgtgtggag tgaaaaaaag tccgcattaa aaaaagtcag tttttatcag tagatgaaaa
     2701 agtaaggttt tgtaaggttg tatattttag caacctcaat aaacctcaac ttctatatta
     2761 gttgtatatt agcatttgtt agcaatctgc gcgtgctttc ctcgtgct
//
LOCUS       DASFSW010000195         2742 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.196, whole genome shotgun sequence.
ACCESSION   DASFSW010000195 DASFSW010000000
VERSION     DASFSW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.196"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            280..2613
                     /locus_tag="U1099_002046"
     CDS             280..2613
                     /locus_tag="U1099_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEM4868322.1"
                     /translation="MNNKIIETLEFHKVRQKIEPYLLTEQGFEELRQLEPMVEVQRIQ
                     QAFDELTDIAQIFVENPYFSLSATSDIGPAMRRLELDTDLNIAELLAVKKVLEVSKSL
                     LDFYGNLENVSLSQLDKLFEKIELFPHLQGSLQSINDAGFVEDFASEKLARIRRKIRE
                     AEDQVRQVMQDILKTKGDMLSDSILASRNGRNVLPVKNTYRNKIAGVVHDISASGSTV
                     YIEPRAVVTLNEEISHLRAEERHELNRILQELSDMLRPHSGVIRNNAWLIGHIDFVRA
                     KHLFAREHQAVVPKLSEKQDIALLNVRHPLIAKPVPNDLYFGSQLTAIVITGPNTGGK
                     TIMLKTLGLTHLMAQSGLPILADKGSRVAIFKEIFADIGDEQSIEQSLSTFSSHMTHT
                     VQILAEADQDSLILFDELGAGTDPQEGASLAMAILDDLRLRGIKTMATTHYPELKAYG
                     IETSGIENASMEFDTNSLRPTYKFMQGVPGRSNAFEIARRLGLSDIIIQSAQSWTDTD
                     SDVNRIIEKLESQTVESRQRLDKIRDVEQENYKMNRALRKLYDELNRERENELNKARL
                     EAKEIVDMALAESEDILKNLHAAASLKPHQIIEAKAELKKLAPEVVDLSKNKVLKKAK
                     IQREAKVGDDIIVTAYGQRGTLTNQLKDGRWEAQVGLIKMTLAKDEFELVKAEKAEQP
                     KKRQVHTVKRANVRGPKARLDLRGKRYEEAMMELDEFIDQALLNNLAQVDIVHGIGTG
                     VIREGVTKYLRRNKQVKEFGYAPQNAGGSGCTIVTFK"
BASE COUNT          783 a          573 c          669 g          717 t
ORIGIN      
        1 tctgagaagc gaggatggac tttctgccca gcctcagcct gttcccttga catataaaag
       61 gaacagagtt ctatctgcat cagagcgaaa cgaactacgt tcgcctattt ccaacctcta
      121 aaggttccca cgaacccttg gagcaagtct gattttgatt ttcattgagt attagttgac
      181 tgttataact ttaatgtaag agattgggaa agactatatt tttatctttt attgttttga
      241 taatgagata aagctaagga aaagactaag taggaacata tgaataataa aattattgaa
      301 acccttgaat ttcacaaggt aagacaaaaa attgagccct atctcttgac ggaacagggc
      361 tttgaagaat tacgacagtt ggagcccatg gtggaagtcc aacgtatcca acaggccttc
      421 gacgagttga cagacatagc gcagattttt gtggaaaatc cctatttcag tctgtcagct
      481 actagtgaca ttggtccagc catgcgtcgt ttggaattgg atacagacct caatatcgct
      541 gaattattag ctgttaaaaa ggtcttggaa gtttctaaat ctctcttgga tttttatgga
      601 aatctggaaa atgtcagtct tagccagctg gataaactct ttgagaagat tgagcttttt
      661 ccacatttac agggctccct ccagtccatc aatgatgctg gttttgtaga ggattttgcc
      721 tcagaaaagc tggctcgtat tagaagaaaa atccgtgaag ctgaagatca agttcgtcag
      781 gtcatgcagg atattttgaa gaccaagggc gacatgctgt ccgatagtat tttggcaagc
      841 cgtaatggac gcaatgtcct tcctgttaaa aatacctatc gcaataagat tgcgggtgta
      901 gtccatgata tctccgcttc tggctcgacg gtttatattg agccgcgggc agtggtgacc
      961 ttgaacgaag aaatcagtca cctacgtgca gaagaacgcc atgagctcaa ccgtatcctg
     1021 caggaattat cggatatgct ccgtccgcat agtggtgtga tccgtaacaa tgcctggctt
     1081 atcgggcata ttgatttcgt ccgtgctaag catctctttg cacgtgaaca tcaagcagtt
     1141 gtacccaaac tatctgagaa gcaggatatt gctcttctca acgttcgaca tccgctcatt
     1201 gctaagcctg taccaaatga cctttatttc ggtagtcagt tgacggctat cgtgataaca
     1261 ggtcccaaca caggtggtaa gaccatcatg cttaagacct tgggattgac ccatctgatg
     1321 gcccagtctg gtttgcctat cttggctgac aagggcagtc gggtagctat cttcaaagaa
     1381 atttttgcgg acattggaga cgaacagtcc atcgagcaga gtttatcgac cttctccagt
     1441 cacatgaccc acacggtaca gattttggcg gaggcggatc aagattccct cattctattt
     1501 gatgagttgg gagctgggac agatccgcag gagggtgcgt ccttggcaat ggctattttg
     1561 gatgaccttc gtctgcgggg gatcaagacc atggcgacca cccactaccc tgagcttaag
     1621 gcctacggga tagaaacctc tggtattgaa aatgccagca tggagtttga taccaatagc
     1681 ctgcgtccga cctataagtt tatgcagggt gttcctggtc gctccaatgc ctttgaaatt
     1741 gctcgccgtc ttggtctatc tgatattatt atccagtcag cccaatcttg gacggataca
     1801 gatagcgatg tgaaccgcat tatcgagaaa ttggaaagtc agacggttga aagtcgtcag
     1861 cgtctggata agattcgtga tgtggagcaa gaaaattaca agatgaaccg agccctccgc
     1921 aagctctatg acgagctcaa tcgtgagagg gaaaatgagc tcaacaaggc acgcttggaa
     1981 gccaaggaaa ttgtagatat ggcattggca gaaagcgagg atattctcaa aaatctccat
     2041 gctgcagcta gtcttaagcc ccaccagatt atcgaagcca aggcagagct gaaaaagttg
     2101 gcgcctgaag tggtggatct gtctaaaaac aaggttctga agaaagctaa aattcagagg
     2161 gaagccaagg tgggcgatga cattatcgtt acagcttatg gacaacgcgg gaccttgacc
     2221 aatcagctca aggacgggcg ttgggaagct caggttggct tgattaagat gaccttggcc
     2281 aaagatgagt ttgagttagt caaggcggag aaggcagagc agcctaagaa acgccaggtt
     2341 cacacagtca aacgtgccaa tgtacgagga ccaaaagccc gcttagatct ccgtggcaaa
     2401 cgctacgaag aggctatgat ggagctggat gaatttatcg atcaggccct tctcaataac
     2461 ctagcccaag tcgatattgt ccacggtata ggtacaggtg tcatccgaga aggggtgacc
     2521 aagtacctac gccgaaacaa gcaggtcaag gagttcggct atgccccaca aaatgcaggt
     2581 ggatcaggct gtactattgt gacgtttaaa taaggaatta gcaaggatag attcagtctg
     2641 ttcttgcttt tatatttctg ttatacaggg aataggatag gtcaaaatac agttcaagta
     2701 cttactaggt tatgctatac tatacttatg aggcaagaaa ta
//
LOCUS       DASFSW010000196         2655 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.197, whole genome shotgun sequence.
ACCESSION   DASFSW010000196 DASFSW010000000
VERSION     DASFSW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.197"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..329
                     /locus_tag="U1099_002047"
     CDS             <1..329
                     /locus_tag="U1099_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEM4868323.1"
                     /translation="KLMQKIEHDQMVEIVKSEEVREIVEEQLKYLDNEALSEDGLIKT
                     YEIDDSSIIHSPMGGISFNVFLNSDRKLRVVFELEKDSNTGKIEYSGGGYSSEVKKLV
                     GEINND"
     gene            322..507
                     /locus_tag="U1099_002048"
                     /pseudo
     CDS             322..507
                     /locus_tag="U1099_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
     gene            611..988
                     /locus_tag="U1099_002049"
     CDS             611..988
                     /locus_tag="U1099_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEM4868324.1"
                     /translation="MKKVMKVIIGILLVIGIGIGGFVYMQHKEHEKMVAIATSEEARA
                     VYEKHMKANDPKALTADGIIKTYEIDTETLEYNPMGGMIVRVYVNGDEDFGFHFNLLQ
                     NEDGKFKSGSYVIKSKLADLLGK"
     gene            1035..1292
                     /locus_tag="U1099_002050"
     CDS             1035..1292
                     /locus_tag="U1099_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868325.1"
                     /translation="MTEYNNLGKGDAVITQYDDIVGRVTLDFDNENGNGEQVYAVVKD
                     PNLPEDQVTEVMVLFRGSTGSNEVLTKPADVWNDWGESILS"
     gene            1387..1776
                     /locus_tag="U1099_002051"
     CDS             1387..1776
                     /locus_tag="U1099_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEM4868326.1"
                     /translation="MKIVVKILVGILIAIAIGIGGFKLMQKIEHDQMVEIVKGEEVRK
                     MVEERLISYDAKALTEEGIIRSYSIDSASIRRNPMGGINFSVYLNNDDTLYINYRIEK
                     IRDDIVKLVGGGKSSDLTRLLGEEANE"
     gene            1769..>2655
                     /locus_tag="U1099_002052"
     CDS             1769..>2655
                     /locus_tag="U1099_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEM4868327.1"
                     /translation="MNNSKTNRDLTDFDRQQVASEEYTPYLKGNEVVITNFFEEELTI
                     GTVRKVLKDATGLDGYVVEEPDGNVIVLFQGSKGPGKEGAAADWMDNDLPMTHNIISN
                     KSEVTPQLQSASRILNQVLKDYPNAQITVYGHSLGSMNAQYALAAVSDIDRIAGAYIY
                     NGPNVYPALTEAGKARVNALKYRIHNYIDQKDFVPIGYSGKDAPGYKSTAGTSEGAIG
                     IVYRVDSKTNLNPIDQHVWGGYQWNADGSLKVKEGSSKLEQHYSNALHHVSSEMYHYA
                     TLKATLSRGGFSSRETIYL"
BASE COUNT          894 a          406 c          632 g          723 t
ORIGIN      
        1 ttaagctgat gcaaaaaatt gagcacgacc agatggtgga gattgtgaag agtgaggaag
       61 tgagggagat tgtagaagaa caacttaaat atctagataa tgaagctctt tcagaggatg
      121 ggctgataaa aacatatgaa atcgatgatt cttctatcat tcatagtccg atgggaggga
      181 taagttttaa tgttttcttg aattcagata gaaagttaag agtggtgttc gaattagaaa
      241 aggattctaa tacaggaaaa attgaatact ccgggggagg gtattcttct gaagtgaaaa
      301 aattagttgg agaaataaac aatgactgaa aagtatactg atcgtgatcg acagaaaatt
      361 gccaacaaag agtattcagc ttacaaattt ggagatgagg taaatatcgg gaccctagaa
      421 caccctagat acatcggcac agtccgtcag gtactcaaag atgcaacagg tttggatggt
      481 tatgttgttg aagagcctga cggcaacgag tgttgtgacg gtgacggtaa cacagtttca
      541 gctgcatcta tgttatacta tgaggtaaga aagcatctta cttgatagga tttcataaag
      601 gaggtctagg atgaaaaaag taatgaaagt tattatcgga attttgttag taatcgggat
      661 tggaatagga ggatttgtat atatgcaaca caaggaacat gagaaaatgg tcgctatcgc
      721 tactagcgag gaggctagag ctgtttacga aaaacacatg aaagcgaatg acccaaaagc
      781 attaacggct gatggaatta taaaaactta tgaaattgac actgaaacac tagagtataa
      841 tcccatggga ggaatgattg taagagttta tgtaaatgga gatgaagact ttggctttca
      901 ttttaacctt ttacaaaatg aagatggaaa gtttaagtct ggaagttatg ttatcaaatc
      961 aaaattggca gatttattag ggaagtagtt catggtaaaa aaaatatgtt aatgaagata
     1021 atcttcagat tgcaatgacg gaatacaata atttaggtaa gggagatgct gtaattacac
     1081 aatatgatga catagtaggt cgtgtcaccc tcgacttcga caatgagaat ggcaatggag
     1141 aacaggttta tgctgttgtg aaagatccaa acctgcccga agaccaggtt acggaagtaa
     1201 tggtactctt tcgaggctcg acaggatcaa atgaagtgtt gacaaaacct gcagatgtgt
     1261 ggaatgactg gggtgaatcg atactctcat agatttgcgc gtctttatat ataaacatcg
     1321 catttatgct atactaggag gtaagaaatg ctcttacttg ataggatttc ataaaggagg
     1381 tttggaatga aaatagttgt taaaatactt gttggcatct taattgccat cgcaatagga
     1441 ataggaggat ttaagctgat gcaaaaaatt gagcacgacc agatggtaga gattgtgaag
     1501 ggtgaggaag tgaggaaaat ggttgaagag aggttaattt cttatgatgc caaagcgtta
     1561 accgaagaag gaataataag gtcctactcc attgatagtg cttctattcg tcgcaatcca
     1621 atgggaggga taaatttttc cgtatatttg aataatgatg atactctcta tataaattat
     1681 aggatcgaaa aaataaggga tgatattgtt aaactggtgg gaggtggaaa gtcatccgat
     1741 ttaactaggt tattaggtga agaggcaaat gaataattca aaaacaaata gagatttaac
     1801 tgattttgat agacagcagg tggcatctga agaatatact ccatatttaa aaggcaatga
     1861 agttgtaatt acaaattttt ttgaagagga actaaccatc ggcacagtcc gtaaggtact
     1921 caaagatgca acaggtttgg acggttatgt tgttgaagag cctgacggca atgtgatagt
     1981 cttgttccaa ggttctaaag gtccagggaa ggaaggcgct gcagcggatt ggatggataa
     2041 tgatttgccg atgacgcaca atattatatc taataagtca gaagtgacgc cccaacttca
     2101 gtctgcttcc agaattctga accaggttct gaaggactac cctaatgctc aaattacagt
     2161 ctacggtcac tcgttaggct caatgaatgc tcaatatgct ctggccgctg tttcagatat
     2221 tgaccgcatt gctggggctt atatctataa cggcccaaat gtttatcctg ctttgacgga
     2281 agcaggaaaa gccagagtga atgctctgaa ataccgtatt cataattaca ttgatcaaaa
     2341 ggatttcgtt cctattggct attcagggaa agatgctccc ggctataaat caactgcagg
     2401 aacgagtgag ggagcaatcg gcatagtcta tcgtgtggac agcaagacta atctcaatcc
     2461 tattgatcaa cacgtgtggg gtggttatca atggaacgcc gacggcagcc taaaagtaaa
     2521 ggaaggttct tcaaaactag aacaacatta ttccaatgct ctccaccatg tctcgtcgga
     2581 aatgtaccac tatgctactt taaaagcaac cctatcccgt ggtggcttca gttcgcgtga
     2641 aaccatctat ctgga
//
LOCUS       DASFSW010000197         2581 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.198, whole genome shotgun sequence.
ACCESSION   DASFSW010000197 DASFSW010000000
VERSION     DASFSW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.198"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..626
                     /locus_tag="U1099_002053"
     CDS             <1..626
                     /locus_tag="U1099_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM4868328.1"
                     /translation="PSAQRSYTVYIDPGHGGRDSGASYGGVHEKNLALSVSNKLRENL
                     LQYGINVLMTRTGDYDVDFKTERSRMTNASNADLFISIHFNATGAGVSNATGIETYWY
                     QYNPEYQPKINKEMHNNPTRLAESEILANKVQESLIKETGAVNRGVRRETFAVLRETA
                     IPAILVELGFMDNPSELQVIKQDSYHTRLAKALAQGVMNWYGAVEGK"
     gene            704..1132
                     /locus_tag="U1099_002054"
     CDS             704..1132
                     /locus_tag="U1099_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM4868329.1"
                     /translation="MEQSSKANSILSSVMLSQAILESAWGTSYLATHGNNLFGIKADA
                     AWTGATIEIITNEYRDGEKKQEKHLFRKYNSWNESVADYAKFFTSTPWRIKNYQSFRE
                     ATDYQQAILALRQSGYATDPKYGEKLRSIIENYKLYLLDN"
     gene            complement(1192..>2581)
                     /locus_tag="U1099_002055"
     CDS             complement(1192..>2581)
                     /locus_tag="U1099_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM4868330.1"
                     /translation="PSRTFYSSYDFKWPKRFSRILLGYITLITIVPMINKTVVSNANI
                     SLNYWRMYVTYGQFGLPLSLASFYEDSKITKPEGYSVPKLNEVLEKYPPETEKQTIRP
                     NIIFIQNESQSDFSNLQGLNMEPDPLSNQHALTDNTIHGTLNVSVFGGGTANTEYEVL
                     TSNPISLLSSNLFPYQQIITQERPSFATYLKNKNYDTVALHPQSGNNYNRNAVYPLLG
                     FNKSYFLDSEPAISSLAPLTIDRGWPSDQFLFNGIKELYTQKGDQPLFSFVVTMQGHG
                     GYPSTEEIYPREVSINGSTSEYLAETEFLTSMKRTDEAFADLITFFSTYKEPTVIVMY
                     GDHQPSLTQEFYAQFMDENNPAAKYSTPLVIWSNFDIRERESTTISPNYLVPYLMDIL
                     SESDYALPRSPYQQFLSDMQIEAPIITSWGNIDNNGQQIEDLSTLPLYQTYLQLEYNS
                     AIDKQPLTDLYE"
BASE COUNT          803 a          427 c          558 g          793 t
ORIGIN      
        1 atccgtcggc acagcgatcc tacacagtct atattgaccc agggcacgga gggcgagact
       61 cgggtgcttc ttacggtggc gtacatgaaa aaaatcttgc attgtctgtt tcaaataagt
      121 tgcgtgaaaa tctcttgcaa tatggaatca atgttttgat gacccgtaca ggtgattatg
      181 atgtagactt caaaacagag aggtcacgaa tgacgaatgc aagcaacgca gacttattta
      241 ttagtatcca ttttaatgcg acgggtgcag gagtttcgaa tgcgactggt atcgaaacat
      301 actggtatca atataatcca gaataccaac ctaaaattaa taaagaaatg cataataacc
      361 ctactcgttt agctgaaagt gagattttag ccaacaaggt ccaggagagt ttaattaaag
      421 aaacaggtgc agttaaccgt ggtgtaagaa gagaaacttt tgcagtcctt cgcgagacag
      481 caattcctgc cattctagtg gagctaggat ttatggataa tccttcggag ttgcaagtta
      541 tcaaacaaga ttcttaccac actcgccttg cgaaagcatt ggcacaaggt gtaatgaatt
      601 ggtatggtgc tgttgaggga aagtagaaat tgcaggtaca ggtattgaaa caggaaaact
      661 ttctaatgat caaaaaagtt tcatagccaa tctagtacct gcaattgaac aaagttcaaa
      721 agctaattct atcctttcga gcgtcatgtt atctcaagcc atcctcgaaa gtgcatgggg
      781 aacgtcatac ttggcgactc atgggaataa tctttttggc atcaaagcgg atgctgcgtg
      841 gacaggtgct acaattgaaa tcattaccaa tgaataccgt gatggtgaga agaagcaaga
      901 aaagcacttg tttagaaaat ataattcatg gaatgaaagt gtagcagact atgctaagtt
      961 ctttacctct actccgtgga gaataaaaaa ttaccagagt ttcagagagg caactgatta
     1021 tcaacaagcg attcttgcgc taagacaatc aggatatgct actgatccga aatatggtga
     1081 aaaattacga tcaattatcg agaattacaa gctttatctt ttagataatt aagttttttg
     1141 ggtgattgat gataaaatag ccttgctcaa aaccgagcaa ggctattttt atcactcata
     1201 taaatctgtt aaaggttgtt tgtcaatggc actattgtat tctagttgta agtaggtttg
     1261 gtaaagtggt agggttgata ggtcttcaat ttgttggcca ttattatcga tatttcccca
     1321 gctagtaatg ataggtgctt cgatttgcat atcactcaaa aattgttggt aaggtgatcg
     1381 aggaagggca tagtcggatt cggataaaat atccatcaaa tatggaacga gatagtttgg
     1441 gctgattgtt gtagattcgc gctccctaat atcaaaattc gaccaaatca ctaaaggggt
     1501 agaatattta gcggcaggat tattttcatc cataaattgt gcgtaaaatt cctgagtcaa
     1561 actaggttgg tgatcgccgt acatgacgat gactgttggc tctttgtaag tgctaaagaa
     1621 agtaattaaa tctgcaaatg cttcatctgt tcttttcatg ctggtcaaaa attctgtttc
     1681 agctaagtat tcagatgttg aaccattaat gctgacttcg cgaggataaa tttcttccgt
     1741 acttggatag cctccatgtc cttgcatggt aacaacaaag ctaaatagag gctggtctcc
     1801 tttttgggta taaagttctt ttattccgtt gaacaagaat tgatcagatg gccagccacg
     1861 atcgattgtt agtggtgcta agctactaat agcaggttcc gaatctaaaa aataagattt
     1921 attaaacccc agtagaggat acactgcatt gcggttgtaa ttatttccgg attgaggatg
     1981 tagagctaca gtgtcgtaat ttttattttt tagataagtt gcgaaacttg gacgttcttg
     2041 tgtgataatc tgctgatatg ggaataaatt agaagataat aaactgattg ggttacttgt
     2101 cagcacttca tactcggtat ttgcagtacc accgccaaag actgagacat tgagggtgcc
     2161 atgaatagta ttgtctgtta atgcatgttg gtttgatagc ggatcaggct ccatatttaa
     2221 gccctgtaag tttgaaaaat cactctgtga ttcattctgt ataaagataa tattaggtct
     2281 gattgtttgt ttttctgttt caggagggta cttctcaaga acttcattaa gtttgggaac
     2341 tgaatatcct tcaggcttag ttattttact atcttcatag aaagaggcga gtgagagtgg
     2401 tagcccaaat tgaccatagg tgacgtacat tctccagtag tttaatgata tatttgcatt
     2461 agagacgaca gttttattaa tcattggaac gattgttatt aaagttatat agcccaataa
     2521 aattctgctg aaacgtttcg gccatttgaa atcataacta gaataaaacg ttctagaagg
     2581 t
//
LOCUS       DASFSW010000198         2572 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.199, whole genome shotgun sequence.
ACCESSION   DASFSW010000198 DASFSW010000000
VERSION     DASFSW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.199"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            43..912
                     /locus_tag="U1099_002056"
     CDS             43..912
                     /locus_tag="U1099_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEM4868331.1"
                     /translation="MELKQAILASEGRTVMAENVPSRSSFLSGVSNAEMERAAGADLI
                     MFNALDLFHPLIAEIPEDVQENPIVWIKKALGRAIGVNLEPVDLEANMVETRAEISIG
                     RIVSPKTLKKATELGFDFICLTGNPGTGVSNQSIFSAISMAKEYFSGLIIAGKMHGAG
                     VDEPVMNLEIAKQFIHSGIDILLVPAPYTVPNFVAEELKEIVDYVRSYNKDQTIENKV
                     LVLTANGTSQDSSDTDTIKKIALTSKACGADIQHIGDSFNGIALPENIFALGQAIRGK
                     RHQLTMLARSIIR"
     gene            944..2197
                     /locus_tag="U1099_002057"
     CDS             944..2197
                     /locus_tag="U1099_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM4868332.1"
                     /translation="MNVQSVQKFAAKISNQRHLIALRDGMATAMPLIIVGSVFMLIAD
                     FPIPAFSAWLSDIGISEFLWKATDATFGIAGLVVTFAVAYHLAKRYDVDRLSAGVLSI
                     ASFVLLTPVLSSDAGNGFPLKYLGTAGLFVGIIVALISTEIFSFFVKRNIVLKMPDTV
                     PPNVAKAFSAIIPGLFIISLWLMILILLKATGIDNIHALIADTVAKPLGLVTQTLPGI
                     LVVIFIQCFFWMFGVHGAQVTGPILEPLLLQNSEANRLAYQAGEELPHIIIYEFLYNF
                     VFSGGAGCVMALAILTFFLSRSQENKALGKLSIAPVSFQVAEPILFGFPTILNIKMAI
                     PFVLAPVVTTIVTYFAMDWGLVAKPIGAVVSWTTPPVIAGFLASGGRISGAVIQIVTI
                     LINMAIYYPFFRMDDKAKLEREQGK"
BASE COUNT          744 a          428 c          566 g          834 t
ORIGIN      
        1 ttacttgatt attcagcatc tgattttttg tcggcctccc ctatggaact gaaacaagca
       61 atactggcgt cagagggacg aacagtgatg gctgaaaatg ttccgagcag atcgtcattt
      121 ctgagtggtg tttctaatgc agaaatggaa agagcagcag gagcagattt gataatgttc
      181 aatgcattgg acttgtttca tccgcttata gcggaaatcc ctgaggatgt tcaagaaaat
      241 ccaattgttt ggataaaaaa agctctcggg cgggcgatag gtgtcaatct tgaacctgtt
      301 gatttggaag caaatatggt tgaaacacgt gctgaaattt caataggtag aattgtttcg
      361 ccgaaaacat taaaaaaagc tacagaactt ggcttcgact ttatttgctt gacaggaaac
      421 ccaggtacag gggtcagtaa ccagtccata ttttctgcaa tatctatggc aaaagaatat
      481 ttttctggtt tgattatcgc agggaaaatg catggagctg gagttgatga gcctgtgatg
      541 aatttagaga ttgctaagca gtttatacat tcgggaattg atattctgct agtaccagcg
      601 ccctatacag taccgaattt tgttgcggag gagttaaagg aaatagtaga ctatgttagg
      661 tcatataata aggaccagac tatagagaat aaagttttag ttttgacagc taatggtact
      721 agtcaggact cgtcggatac cgatacaatt aagaaaattg cattgacctc taaagcttgt
      781 ggtgcagata ttcaacatat tggggattct tttaatggaa tagcacttcc tgaaaatatt
      841 tttgctttag gtcaagcgat tagagggaag cgtcaccagt taacaatgtt ggctcgttct
      901 attattcgat aaaatatagt aataatatat taaggagact attatgaatg ttcaatcggt
      961 tcaaaagttt gctgcaaaaa ttagcaacca aagacattta attgccttgc gtgacgggat
     1021 ggctactgca atgcctctca ttattgttgg ctcagttttt atgttaattg cagattttcc
     1081 aattcctgcg ttttcagcct ggctttctga tatagggatt tcagaatttt tatggaaagc
     1141 aactgacgct acttttggga tagctggctt agtagtaaca tttgctgtcg cttatcatct
     1201 agctaaacgg tacgatgttg atagattatc tgcaggagtg ctttctatag cgtcatttgt
     1261 gcttttaacc ccagttttat catccgatgc aggaaatggt tttcctttga aatatttagg
     1321 aactgctggc ttatttgtag gcatcattgt tgccttaata tccactgaga tattcagttt
     1381 ctttgttaaa agaaacattg ttttgaaaat gcctgatact gtaccaccga atgttgcgaa
     1441 ggcattttca gccattattc caggtttgtt tattatttcg ctttggttga tgatactgat
     1501 tcttctaaaa gcaacaggaa ttgataatat tcatgctttg atcgctgata ctgttgcgaa
     1561 accacttggt ctggtgacac agacactgcc gggaatattg gttgtgatct ttattcagtg
     1621 tttcttttgg atgtttggtg ttcacggggc tcaagtcact ggcccgattt tggaaccact
     1681 tttattacaa aattcagaag ctaaccgtct agcctatcaa gcaggtgaag agttgccaca
     1741 tatcattatt tatgaatttc tttataattt tgttttttca ggtggagcag gctgtgtgat
     1801 ggcattggcg attttaacgt ttttcctttc tcgaagtcaa gaaaataaag ctttaggaaa
     1861 attatcaatt gcgccagtta gtttccaagt ggcagaaccg attctatttg gttttccaac
     1921 aattttgaat ataaaaatgg ctatcccatt tgtcttagca ccagttgtaa caaccattgt
     1981 cacttacttt gcaatggatt ggggactggt tgcaaaacca attggagcgg ttgtttcgtg
     2041 gacaacgcca ccagtaattg cgggtttcct agcctcaggt ggtagaatta gtggagcggt
     2101 tattcaaatc gttacgattt tgattaacat ggccatttac tatccattct tccgaatgga
     2161 tgataaggcg aaattggaac gcgaacaagg aaagtagttt gcttacttcc tgacataaca
     2221 aagcgataga aagaaaagcc cttcgcaagg actatttggt tcaggtgcga aggacttttt
     2281 ttattaagga tgtggaatat atgaaaaatc aaattaagtg ttgactgcaa ggcttaaaga
     2341 gagttcggat ttttcagaaa atttccattt ttctgttgac aagtgaaaat aagtagggta
     2401 tcattgtaaa taattaaaat actgaaagga aaaagtcaaa tgaaacctgt cactacaaaa
     2461 ttctttgctt tgttgttgcg acggtcgggc tagtgctgaa agaatgcatt agtcctgttt
     2521 ggcttaccaa gcgggcactg ttgacaacat ctcgcaggta atagtcctcg ag
//
LOCUS       DASFSW010000199         2536 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.200, whole genome shotgun sequence.
ACCESSION   DASFSW010000199 DASFSW010000000
VERSION     DASFSW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.200"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..2502
                     /locus_tag="U1099_002058"
     CDS             <1..2502
                     /locus_tag="U1099_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEM4868333.1"
                     /translation="KQGKTLLYVAGVSLICAVALVTYQASNYREQSNHQKTTSIETSQ
                     SKQSKEIPSDSKKEVPGIDKATDDGFLLTDESQIEEKTDLGIIVNHGDHKHFFFYSDL
                     KNTKWAYLIPENYQEKNHSSQPKRSQLSSGSKQIEDEYVFDPKDIVAEDANGYTVRHG
                     DHYHYILKSSLGGTTHRQLTSDSRQSPTLPIVHQQEGIPGIDFQTSDGFLFDGQNISG
                     VTETGILVRHGKHLHLIHFETLKRSKWSYLVNQYKPNVESDKKAQPEVENTEYQTKLD
                     YLAKELNLEPSRFKKVIVDGQIGLEYPHGDHTHIVLLKEIDTTKPFESPEDRILKQKD
                     GETLEQRKERLIKQYMERFKVKREDITIDGNYMSVRHGDHAHVYKIDPSLPDDPERDV
                     KTETVNLAIEKQLVYGPFYTEGSTENLTRNGVHQKYHPEGLQDIKNFILVTFSTNSDF
                     GDFVVNGKKTKRVYYLVRKDLNWEDLNIAYPQTLQQKGRVFNGWNATLPKSGKMAREH
                     QSFYADFDNVYRKPTKNIYTPSDDVSNIDLSDYVPVKYSAIANGRLKLNGEIRAGFIY
                     FVKSDLTWKQAKEQGLVVPEPVPSKDYEFIEFRTVITGGEKDTDYVSATTSLAAFGTT
                     APRIGPYIAENMDNPTDINDPSRHPNYYWHDPKNYVALAFRAGDGGQLQTHLGTSKTV
                     VYLVRKGLTLNQAGIFPPSLKSDTGYKRDYTKPVIPNVAWDTPILEDTVYDIHFDKVE
                     SDSKDGTVEPGDTWLPGNPLPNTPDVPNGEEDADSWLDDLLTPSPEATDIETVTEAET
                     TTEDTKATESSTAAPIDIPKKSSTEEEPSEDFIFP"
BASE COUNT          873 a          419 c          539 g          705 t
ORIGIN      
        1 aaacaaggta agactttgct ttatgtagca ggagtatcgc ttatttgtgc tgtagcattg
       61 gttacttatc aagcgtctaa ctatagagag caatcgaacc atcaaaaaac aacaagcata
      121 gaaacatcgc aatcaaagca atcaaaagag attccttcag attcgaaaaa ggaagttcca
      181 gggattgata aggcgacgga tgatggtttt ttactcactg atgagtcaca aattgaggaa
      241 aaaactgatt taggaattat tgtcaatcat ggcgatcata agcacttttt cttttattca
      301 gatttaaaga atacaaaatg ggcttattta attcctgaaa actatcaaga aaaaaatcat
      361 tcttctcaac ctaagagaag tcaattatcg tcaggctcga aacagatcga agatgaatac
      421 gtttttgatc ctaaagatat tgtcgcagag gatgccaatg gctatactgt tcggcatggg
      481 gatcattatc actatatttt aaaatcaagc ttaggtggca cgacacacag acaattgacc
      541 tctgatagtc gtcaatcgcc aactcttccc atcgttcacc aacaggaagg gattccgggg
      601 attgattttc aaacgagtga cggtttcttg tttgatgggc aaaatatcag cggtgtaacg
      661 gaaaccggga ttctagtaag gcatgggaaa catttgcatc ttattcattt tgaaacattg
      721 aaaaggagta aatggtctta tcttgtgaat cagtataaac caaatgttga atcggataag
      781 aaggcacaac ctgaggtaga aaatactgag tatcaaacca aattggacta cttagcaaaa
      841 gaactgaatt tagagccatc acgttttaag aaagtcatcg ttgacggtca aattggatta
      901 gagtatccgc atggagatca cacacatatt gttttattaa aggaaattga tacaacgaag
      961 ccgtttgaaa gtccggaaga tcgtattctt aaacaaaagg atggagaaac tcttgaacag
     1021 agaaaagaac gtctcattaa acagtacatg gagcgtttta aagtaaaacg tgaggacatt
     1081 acaattgatg ggaactatat gagtgtacgc catggagacc atgcgcacgt ttataaaatt
     1141 gatcctagtt tgccagatga tcctgagcgt gatgttaaga ccgaaacggt aaatcttgct
     1201 attgagaaac aattagtata tggacctttt tatacagaag gttcaacaga aaatttaaca
     1261 cgtaatggtg tccatcaaaa gtatcatccg gaaggtcttc aggatattaa aaatttcatt
     1321 ctagtaacat tcagtacaaa tagtgacttt ggtgattttg tcgttaatgg taaaaaaacg
     1381 aagagagttt attatttggt tcgtaaagat ttaaattggg aggatttgaa catcgcctat
     1441 ccgcagaccc ttcaacaaaa gggacgcgtc tttaatggtt ggaatgctac tttgccaaag
     1501 tctggcaaga tggcaagaga acatcagagt ttttatgctg attttgataa tgtttatcgt
     1561 aagccgacaa aaaatattta cactccaagt gatgatgttt caaatattga tttatcagac
     1621 tatgtacctg ttaaatatag tgcgatagcc aatggtcgtt taaaattaaa tggtgagatt
     1681 cgagcagggt tcatatactt tgtgaaatct gacttaactt ggaaacaagc taaggagcag
     1741 gggcttgttg taccagaacc ggttccaagc aaagattatg aattcatcga gtttaggaca
     1801 gttattaccg gtggagaaaa agatacagat tatgttagtg ccacaacgag cttggcagca
     1861 tttgggacaa cagctcctcg tatagggcca tatattgccg aaaatatgga taatccaacg
     1921 gacatcaatg atccaagtag acatccaaat tactattggc atgatccaaa gaattatgta
     1981 gctcttgcat ttcgagcagg tgacggcggt caacttcaga cacatttggg aactagtaaa
     2041 acagttgttt accttgttag aaaaggatta acactgaacc aagctggaat ttttccgcca
     2101 tctttgaaga gtgatacagg ttataaacga gattatacaa aacctgttat tccaaatgta
     2161 gcatgggata cacctatttt ggaagatact gtctacgata ttcatttcga taaagttgaa
     2221 agtgatagca aagatgggac agttgagcca ggtgatactt ggttgcctgg gaatccattg
     2281 ccgaatactc cagatgtccc taatggagaa gaggacgcag atagctggtt agatgatttg
     2341 ttgacaccta gtccagaagc gacagatata gaaactgtaa cagaagctga aactacaaca
     2401 gaagatacaa aagcaactga aagtagtact gctgctccca tagatatccc taaaaaatca
     2461 tcaacagagg aagagcctag tgaggatttt atatttcctt gatgaaatag atatgagtga
     2521 tttgaaatag tggtct
//
LOCUS       DASFSW010000200         2514 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.201, whole genome shotgun sequence.
ACCESSION   DASFSW010000200 DASFSW010000000
VERSION     DASFSW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.201"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            6..401
                     /locus_tag="U1099_002059"
     CDS             6..401
                     /locus_tag="U1099_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868334.1"
                     /translation="MRGRLTEIKGEDTILSTRLSVEAIGVTLQEICGEWKTTPEQITS
                     NSGALARFDDSADIQIVISNKAGLFTSSIYGVQVYVDDLGDKREVQLVALGNSGLSKI
                     LYGSQVVKMSESLKRRDMIAERIMRYSAQ"
     gene            423..1064
                     /locus_tag="U1099_002060"
     CDS             423..1064
                     /locus_tag="U1099_002060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868335.1"
                     /translation="MVGALGFLYLKTYNGAKNILTQETTINALIGGETLGMDYFLERY
                     ITISLSENPNLFGEDRNSFLKLLYAFKDFLSESNTELKIEKYKQLCQDYATDEYKGHA
                     FLVFLKDIFKIFGTEHSAFYAYILRAYVVFMLKQYSNLDITQDSEAEITYILSEIITN
                     WEHICTYEDEISKVATVWALLAFYVDIDQRYINEIGQSIFSFLTEEQLKLLND"
     gene            1076..1243
                     /locus_tag="U1099_002061"
     CDS             1076..1243
                     /locus_tag="U1099_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868336.1"
                     /translation="MANTLKSKVVRGELVEVVEGSGFLGGSDYQLVVGGQIKAQSKDL
                     NYILSEFDRIW"
     gene            1598..2446
                     /locus_tag="U1099_002062"
     CDS             1598..2446
                     /locus_tag="U1099_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEM4868337.1"
                     /translation="MNDFITKYLSQFNIEAIVTAGLNKVLSLVLLFVAFYIIKKIAKA
                     SVKKVLVPSLKVSTQDIGRQKTISRLVESILNYLLYFILIYCILSILGLPVSSLLAGA
                     GIAGVAVGLGAQGFLSDLVNGFFILIERQFDVGDVVKLTNGPITISGTIVSMGIRTTQ
                     VRDADGTLHFIPNRNILVVSNQSRGDMRAQIDIPLKFNTDLEQVYRVIEEVNLREISN
                     FDQITGVTVLGPQNAISGQFVFRVNLFVANGQQNKIYHQFFGFYQDALRQAGIDLPSS
                     GPTVLK"
BASE COUNT          790 a          390 c          514 g          820 t
ORIGIN      
        1 ttgctattcg aggtaggctt acagagatta aaggtgagga tactatcttg tctacacgat
       61 tatcagttga ggctattggt gtgacccttc aagagatttg cggtgagtgg aaaactacac
      121 cagaacaaat cacgagtaat agcggagcct tagctcgatt cgatgattca gcagatatac
      181 aaattgttat aagtaacaaa gcaggattat tcacatctag tatatatggt gtgcaggttt
      241 atgttgacga tttaggcgat aaacgagaag tacaacttgt agctcttgga aattcaggac
      301 tttctaaaat tctatatggc tcacaagttg tgaaaatgtc tgaaagtctc aaacggcgtg
      361 acatgattgc tgaacggatt atgcggtatt cggctcaata aaagtgagag gaggcgtgtt
      421 gtatggtagg agcattaggt tttctatatc taaaaaccta caatggagca aaaaatatat
      481 tgactcaaga aacaacaata aatgctttaa ttggtggtga aactttagga atggattatt
      541 ttctggagag atatattacc attagtctat ctgaaaaccc caatctattt ggagaagacc
      601 gcaattcttt tttgaaacta ttgtatgctt tcaaagattt tttgtcggag tcaaatactg
      661 aattaaaaat tgaaaaatac aaacaattat gtcaggacta tgcgactgac gaatataaag
      721 gtcatgcctt cttggtgttc ctgaaagata ttttcaaaat tttcggaaca gaacattcgg
      781 ctttttatgc gtacatacta agagcgtatg ttgtatttat gctgaaacaa tacagtaatt
      841 tagatattac tcaagatagt gaggctgaaa taacatatat acttagcgaa attattacaa
      901 actgggaaca tatatgtaca tatgaggatg aaatttcaaa agtggcaaca gtatgggctt
      961 tattggcttt ttatgtagat attgaccaac gttatataaa tgaaattgga caaagtatct
     1021 tttcattctt aacggaagaa cagcttaaat tattaaacga ttaggagaaa ttaaaatggc
     1081 aaatacatta aaatcaaaag ttgtacgcgg tgaacttgtt gaagttgttg aaggttctgg
     1141 ttttttagga ggttcggact atcaattggt tgttggcgga caaattaaag cccaatcaaa
     1201 agatttgaac tatatcttaa gtgagtttga tagaatttgg taattatatt tacctaagta
     1261 acgacaaagc aaaagacagt cataattcga ctgtctttta agttttcata agatatatga
     1321 actcggattg tttagattgc tgagtattag gttctcattg tcgttttttg taatattagg
     1381 tatgtgaact ttagttatat cgattgatag catatcagag gaattgaaat ctatcaatat
     1441 aaagccgaac aaaaaaagga cagcatagca tttgccgtcc ttttgattac tcgccgtaag
     1501 gttttatact ttcctaataa ttattttaat aggtttgcat atatcaccct tggctgtctt
     1561 tttgttcttt tagggtgttt tctgctataa ttatctcatg aatgatttta ttaccaagta
     1621 tttgtctcaa tttaatattg aggccattgt gacagcaggt ctaaataagg ttttatcgct
     1681 tgttttactc tttgttgcat tttacattat caagaagata gccaaagctt ctgtgaaaaa
     1741 ggttttggtt ccctctctaa aggtatctac acaagacatt ggtcgtcaga agaccatcag
     1801 tcgtctggtg gaaagcatct taaattacct actctacttt atccttatct actgtatttt
     1861 gagtatcctc ggtttaccgg tatccagtct tcttgcagga gccgggattg ctggggtagc
     1921 tgttgggctg ggcgcacaag gttttctctc agacttggtc aatggattct ttatcttaat
     1981 tgaacgacag tttgacgtcg gagatgtggt taagttaact aacgggccca ttaccatttc
     2041 aggaaccatc gttagcatgg gaatccgaac aacacaagta cgtgatgcag atgggacatt
     2101 gcattttatt cccaatcgca acattctagt ggtgtccaat cagtcaagag gggacatgcg
     2161 tgctcaaatc gatattccgc tcaaatttaa taccgatttg gagcaagtct atcgcgtaat
     2221 agaagaggtc aatttgagag aaatcagcaa ttttgaccag attacaggtg tgactgtatt
     2281 aggtccccaa aacgctattt cgggacagtt tgtatttcgt gttaatcttt tcgtagccaa
     2341 cgggcaacag aacaagatct atcatcaatt ctttggtttt tatcaagatg ctctgcgtca
     2401 agcaggaata gatttacctt caagtgggcc aactgtttta aaataaggag tttattatgc
     2461 aaacaaaaga ttttgtcgtt gaaaatgggc agatgtatta taagaagaaa ccct
//
LOCUS       DASFSW010000201         2505 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.202, whole genome shotgun sequence.
ACCESSION   DASFSW010000201 DASFSW010000000
VERSION     DASFSW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.202"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            26..784
                     /locus_tag="U1099_002063"
     CDS             26..784
                     /locus_tag="U1099_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010019037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HEM4868338.1"
                     /translation="MPLIVKKNNEEIFISLALEMVELIKENNEKNEKTVIICPVGPVG
                     QYPHFVRLVNQFKIDLSNVWFINMDEYLLEDLHWIDVVHPLSFRGFMQKNVYSQIHPT
                     LIMSEEQRIFPDPLDIDSISNKIKELGKIDLCIGGIGLNGHIAFNEPDSTLTVQEFLK
                     LGSRVLPISVETKVMNGLTVLKGAVEEVPNYCVTIGMSEIFQAKKIRLAVFRDWHHAV
                     IRKTVCSKPDTGFPSTLMQIHTNCSIYLNESLAN"
     gene            945..1124
                     /locus_tag="U1099_002064"
     CDS             945..1124
                     /locus_tag="U1099_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM4868339.1"
                     /translation="MKQASYPIQGVTCASCAMTVEKALNKLDGIQNASVNLATEKTNI
                     QYDPAHLSLSDIDSC"
     gene            1273..2020
                     /locus_tag="U1099_002065"
                     /pseudo
     CDS             1273..2020
                     /locus_tag="U1099_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            2077..>2505
                     /locus_tag="U1099_002066"
     CDS             2077..>2505
                     /locus_tag="U1099_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4868340.1"
                     /translation="MFSLSITISVLVIACPCALGLATPTAIMVGTGKGAENGLLFKSG
                     QTIKTLQGVNTIVFDKTGTITEGKPQVTDIHLLSTKNREQVLQLAASSEQYSEHPLAQ
                     ALLRSAEEESIPLLASQDFQALSGRGLSVTIAEQTIYLGNE"
BASE COUNT          779 a          493 c          513 g          720 t
ORIGIN      
        1 aagtgaatta gaaaagcata gtagaattcc attgattgta aaaaagaata atgaggaaat
       61 ttttatttca cttgctctag aaatggtaga attaataaag gaaaataacg aaaaaaatga
      121 gaaaactgtc ataatttgtc ctgttggacc agttggtcag tatccgcatt ttgttagatt
      181 agtgaatcaa tttaagattg atttgtctaa tgtttggttt attaacatgg atgaatattt
      241 gttggaagat ttgcattgga tagacgtagt acaccctctt agttttcgag gatttatgca
      301 gaaaaatgtc tacagtcaaa tacatccaac tcttattatg tcagaagaac aaagaatttt
      361 tccagatcct cttgatatag actcgatttc aaataaaatc aaagaattag gtaaaatcga
      421 cttatgtatt ggtggaattg gcctaaatgg gcacattgca ttcaatgaac cggattcaac
      481 attaacagtt caagaatttt taaaattagg gtcgcgagtg ttacctattt ctgtcgagac
      541 aaaggttatg aatggtttga cggtattaaa aggagctgta gaagaagtgc ctaattattg
      601 tgtaacaata ggaatgagcg agatttttca agcgaaaaaa attcgacttg cagtttttcg
      661 tgattggcat catgcggtca tacgtaaaac agtttgttct aagccagata ctggatttcc
      721 atcaactcta atgcaaatcc atacaaattg ttctatctat ctaaatgaat cgttagcaaa
      781 ttaacctatg ttcaaacaca tacagaatat atataagttt attacccttt tgatcagata
      841 cctaatgaga gaagattctc tcattttttt aatcaaaatt gtttacaaat gtagtcggat
      901 gttgtataat ttgtttacaa atgtagtcag aaaaggagta atctatgaaa caagcaagct
      961 atcccattca aggcgtgacc tgcgcttcct gtgctatgac agtcgagaaa gccttgaata
     1021 agctagatgg aatacaaaac gccagtgtga atctagcaac ggaaaaaaca aacattcagt
     1081 atgatccagc ccatctgagc ttatcagata tcgacagctg ttgaaaaagc aggttataaa
     1141 gcggtactgc caaaaacaca agagtcagaa gctcaaccaa gcggaaaaga acaacacaaa
     1201 aatgatttat ggcacagatt tatctggtca accattttca cgcttccact actctacatt
     1261 gcaatgggac ctatgttacc aggtggggga ttgccattgc cagctatctt acaccagccg
     1321 ctagtttttg ccctagttca gctcattctt gtattaccag tcctatatgt tggtcgtgca
     1381 ttttatcaaa aagggtttaa aaccctgttt gccggtcatc ccaatatgga tagtttgatt
     1441 gccgttggga caagtgctgc cttgtttcaa gggatcatta tgactgacga ttttgattgc
     1501 taccaaccaa gtagccaccc aacatggaca tcctgagctc tattttgaat cggcagctgt
     1561 tattttaacc cttatcactc ttggaaaata catggaggca ttatcaaaag agcgtacttc
     1621 agaagctatc agtaaactga tgaatctcgc ccctaaaact gcacgcgttc tctgtgaagg
     1681 tcaagaagtg accctcccct tagaccaagt caaagtagga gatgtccttc aagtccgccc
     1741 tggtgaacag attccagttg atgggataat agtagaaggc caatcgacaa ttgatgaatc
     1801 catgctgaca ggggaaagtt tgcctgttaa aaaaacatcg gggatacagt cgttggtgcg
     1861 actctcaatc aacatggtgc tttccaaatg gaagcgacaa aagttggagc agataccaca
     1921 ttgtcccaaa ttattcgttt ggtggaagaa gcacaggggt caaaagctcc gattgcgcag
     1981 ttggctgacc gcgtttcagc agtctttgtt ccaattgtaa tgggattagc cgtcctttct
     2041 ggccttgctt ggtacttctt gggacaagag ccctagatgt tctcactttc cattaccatc
     2101 tccgtccttg tcattgcctg cccgtgtgcc ttgggccttg ccacaccaac cgctatcatg
     2161 gttgggactg gaaaaggggc agaaaatggt cttttgttca agtctggtca aactattaaa
     2221 accctacaag gtgtgaacac cattgtcttc gacaagactg ggaccattac tgagggcaag
     2281 ccacaagtga cagacatcca ccttttgtct actaaaaatc gtgagcaagt tctccaactg
     2341 gcagcaagca gtgaacaata ctccgagcat ccattggctc aagccctctt acgttcagca
     2401 gaagaggaat caattccctt acttgccagc caagattttc aggcactttc tggtcgtggt
     2461 ctttcggtaa caatagcaga gcagaccatc tatctgggaa atgaa
//
LOCUS       DASFSW010000202         2463 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.203, whole genome shotgun sequence.
ACCESSION   DASFSW010000202 DASFSW010000000
VERSION     DASFSW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.203"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..439)
                     /locus_tag="U1099_002067"
     CDS             complement(<1..439)
                     /locus_tag="U1099_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM4868341.1"
                     /translation="MKRILFISPTTSMDNGAEKSIYYLMKHLIQLGHTVINVTHAIGG
                     EPQDKHEQLYKMIGVKNYYLPTVKWWWPDAPGGEILNREEATSYYRQNVQDIAEIIDQ
                     NEIDLVISNTVNVFQGAIAASCQKVPHFWLIHEFPKNEFAYYAD"
     gene            complement(439..2421)
                     /locus_tag="U1099_002068"
     CDS             complement(439..2421)
                     /locus_tag="U1099_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM4868342.1"
                     /translation="MFYILELTKNIAKFFWRIPKYIKRTLLALQRDKQRRNNYSDIKN
                     RYLIYTIYEHQSSLQDYKVIFLEALAKISRDVLIVVNGKLPQADINRLAQYGKVLERE
                     NEGYDVAAFRHGIIHTGKEALQQYNQLILVNDTNIGPFRDLEEVFSEFNSDQLDFWGI
                     SMGEEQLDFTGYNPYGKIPKHLQSYFVVIENSLLRYEGFYDYWEKLSDTDSRNKAIGK
                     HETVFAKYFYDRGFKYDALIKDTKDSALYIHPLKLLKQGCPLVKYSAFRNYDREQYFW
                     HGLERESEIPDLMEYIAKETDYPIEVVSSILEDFKTRENQSYILIIDGVENIIPQCTR
                     YRVLNKAEQLRELGYTVRVINNSLVQLQDAQFASHIIIYRAPFNDMLKEICRAAHIKN
                     RPVYFDIDDLVFDTKFTDELEFTQGLSKREKKGYDTSVLAYKKMLSLCDYAITSTSKL
                     KDELEQYKNKVILNRNVMSKELVERSLQVKKHSNDNKVKIGYFSGSITHNENFDLISQ
                     ALLHLLQKYPQVELHIVGYLDIPKPFQKFKKQIVSHEYVDWRKLPILISQVDINLAPL
                     VTTTFNEAKSEIKWIEAAAVKVVTVASNLGAFEEMIQDGVTGVLADDNEWESKLERLI
                     LEQDLREQIAENAFEFVMNHCTTANRINDFLKEELV"
BASE COUNT          779 a          487 c          346 g          851 t
ORIGIN      
        1 tatcagcata ataagcaaac tcatttttag gaaattcatg aattagccag aaatggggaa
       61 ccttttgaca tgaggcagca attgctcctt gaaaaacatt aacagtattt gaaataacta
      121 agtctatctc attttgatca ataatttcag ctatgtcttg aacattctgg cgatagtagc
      181 ttgtagcctc ttctctatta aggatttctc cacctggtgc atccggccac caccacttga
      241 ctgttggcaa atagtaattc ttaaccccta tcattttata aagttgctca tgcttatctt
      301 gtggttcccc tcctattgca tgagtaacat ttataactgt gtgtcccagt tgaataaggt
      361 gcttcattag ataataaata gacttctccg caccattatc catactagtc gtaggagaaa
      421 taaacaaaat acgtttcatt agactaactc ttctttcaaa aaatcattaa ttctatttgc
      481 tgtagtgcaa tgattcatta caaactcaaa agcattttct gctatttgtt ctctcaagtc
      541 ttgttcaaga atcaatctct ccaatttact ttcccattcg ttatcatctg ctagaactcc
      601 agtcactcca tcttgaatca tctcttcaaa tgctccaaga ttacttgcaa ctgtcacaac
      661 tttaactgct gccgcttcta tccatttaat ttccgacttt gcttcattaa aggttgtggt
      721 tacaagcggc gccaagttaa tatcaacttg tgaaattaaa attggaagct ttctccaatc
      781 aacatactca tgactcacaa tctgtttttt gaatttttgg aatggtttcg gaatgtctaa
      841 atatcctact atatgtaatt ccacttgcgg gtacttttga agtagatgta gcaatgcttg
      901 actaattaaa tcaaaatttt cgttgtgtgt aattgaccct gagaaatatc ctattttaac
      961 tttattgtcg ttcgaatgtt tcttaacttg taaactacgc tcaactaatt ctttagacat
     1021 gacgttgcga ttcaagataa ccttgttttt atattgttca agctcgtctt tcaatttcga
     1081 agtcgatgtg atagcatagt cgcagagtga aagcattttt ttgtaagcta aaacacttgt
     1141 atcatagcct tttttctctc tcttgctcaa accttgggta aattcaagtt catctgtaaa
     1201 ttttgtatca aaaactaaat catcaatatc aaaataaaca ggtctgttct ttatgtgtgc
     1261 tgccctacat atttctttca gcatatcatt gaatggagca cgataaataa taatatgact
     1321 agcaaactgt gcatcctgaa gttgtacaag agaattattt atcactcgaa ctgtataacc
     1381 aagctctcgc aattgctcag ccttatttaa cacacgataa cgtgtacatt gtggaataat
     1441 attttcgaca ccatcaataa tcaaaatata agattgattt tctctagttt taaaatcttc
     1501 taatatagaa gacaccactt caatcggata atctgtttcc ttagcaatat actccatcaa
     1561 atccggaatc tcggattctc tttctaaacc atgccaaaaa tattgttctc tgtcataatt
     1621 tctaaaggcc gaatacttaa ctaatggaca accttgcttt aataatttaa gcggatgaat
     1681 atacaatgca ctatctttcg tgtctttaat caatgcatca tacttaaagc ctcgatcata
     1741 aaaatatttt gcaaacacag tttcatgttt tccaattgcc ttattacgag aatccgtatc
     1801 agagagcttt tcccaataat cataaaatcc ttcataccta agcaagctat tttcaataac
     1861 aacaaaatat gattgtaagt gtttaggaat tttaccataa gggttataac cagtaaagtc
     1921 tagttgctcc tcacccatgg aaattcccca aaagtctagc tgatcggaat taaactcaga
     1981 gaaaacctct tccaaatctc taaaaggacc aatattagta tcattaacca agatgagctg
     2041 attatactgt tgtaacgcct cttttccagt atgtattatc ccatgtcgga aagccgcaac
     2101 gtcatacccc tcattttctc tttccaaaac cttaccatat tgtgctaaac gatttatatc
     2161 agcctgtgga agtttaccat ttacaacaat taaaacatct cgagaaattt tagccaatgc
     2221 ctctaaaaaa attactttat agtcctgtaa agacgattga tgttcataaa ttgtgtaaat
     2281 caagtaacga tttttgatat ctgaataatt atttctacgt tgcttatcac gttgcaaagc
     2341 tagtagagtc cgctttatat attttggaat tctccagaaa aactttgcaa tatttttggt
     2401 aagctctaat atataaaaca atagcttgta tttacctgat aagataaata atcgcgttgt
     2461 gaa
//
LOCUS       DASFSW010000203         2460 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.204, whole genome shotgun sequence.
ACCESSION   DASFSW010000203 DASFSW010000000
VERSION     DASFSW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.204"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            106..1512
                     /gene="lacG"
                     /locus_tag="U1099_002069"
     CDS             106..1512
                     /gene="lacG"
                     /locus_tag="U1099_002069"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM4868343.1"
                     /translation="MVKTLPKDFIFGGATAAYQAEGATQTDGKGRVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLELSEQFGVNGIRISIAWSRIFPTGFGAVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPETLHSNGDFLNRENIEHFVNYAAFCFKEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHAKAVKLYKDAGYSGEIGVVHAL
                     PTKYPYDPSNPDDVRAAELEDIIHNKFILDATYLGYYSEKTLEGVRHILKVNGGELDL
                     RDEDFAALDAAKDLNDFLGINYYMSDWMRAHDGETEIIHNGKGEKGSSKYQIKGVGRR
                     ESPVDIPKTDWDWIIYPQGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDGTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            1577..>2460
                     /locus_tag="U1099_002070"
     CDS             1577..>2460
                     /locus_tag="U1099_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEM4868344.1"
                     /translation="MLELKNENLTVQFSELGGQIISIKDKDGIEYLWQGDPTYWSGQA
                     PVLFPICGSLRNDWAIYEPAERPTFTGTIPRHGLVRKMLFKNVKNTENSLEFSISSNE
                     ETVKNYPFEFELSINYSLFGKTIRTEYRMKNLEGHRKLPYFIGGHPGFNCPLLDGESY
                     EDYYLEFEKVESCTVPRSFPETGLLDLRDRRPFLENQKTLDLSYKLFEHDAITLDQLA
                     SRKVTLKSKNHQKKLSIAFDDFPYLVIWSTTNQSPFIALEPWSGLSTSLEESDIFNAK
                     RNISYVAPKEVDTKYFDI"
BASE COUNT          807 a          418 c          513 g          722 t
ORIGIN      
        1 tacaagcact tcacttgtga atacaggttc ttaatcaaaa ggggagggag ttatcatctc
       61 caactcctac ccctttattt atttttgata gaagaaggta ggcatatggt taaaacatta
      121 ccaaaagatt ttatatttgg tggagcaact gctgcatatc aagcagaggg tgctacacag
      181 acggatggaa aaggtcgtgt tgcatgggac aagtatctag aagataatta ctggtacact
      241 gcggaaccgg cttctgattt ttataatcgt tatccagttg atttagaact aagtgaacaa
      301 tttggggtaa acggcattcg tatttcaatt gcgtggtctc gtattttccc aacaggcttt
      361 ggagcagtga atccaaaggg agtagagtat taccataaat tatttgcgga atgccataaa
      421 cgtcatgtag aaccatttgt aacgcttcac cattttgata caccagaaac gctacattct
      481 aatggtgact ttctaaatcg agaaaatatt gagcacttcg tcaattacgc agcgttttgc
      541 ttcaaagaat tccccgaagt taattactgg acaaccttta atgaaatcgg tccaattggt
      601 gatgggcaat atctagttgg gaagttccca ccagggattc agtatgactt agctaaggta
      661 ttccaatctc accacaatat gatggtttca catgccaagg cagttaagct ctacaaggat
      721 gctggttata gtggagaaat tggtgttgta cacgcattgc caacgaaata tccttatgat
      781 ccaagcaacc ctgacgatgt acgtgcagcg gaactagaag atattattca taataaattt
      841 atcctagacg caacatactt gggttactat tctgaaaaaa cgcttgaagg cgttcgacat
      901 attctgaaag taaatggtgg ggaattagac cttcgtgatg aagacttcgc tgccttagat
      961 gccgcaaaag atttgaacga cttcttgggc atcaattact atatgagtga ttggatgaga
     1021 gctcatgatg gagaaactga gattatccat aatggtaaag gggaaaaggg aagttctaag
     1081 tatcaaatca aaggagtcgg tcgtagagag tctccagttg atattccaaa aacggactgg
     1141 gattggatta tctatccgca aggcctttat gatcaaatca tgcgtgtaaa aaatgactat
     1201 ccaaactata agaaaatata catcactgaa aacggcctag gctataaaga tgagtttgta
     1261 gatggtacag tctatgatga tggtcgaatc gactacgtga aaaagcattt agaggtgatt
     1321 tcagatgcaa tctcagatgg tgccaatgtt aaaggttatt tcatctggtc cttgatggat
     1381 gtattttctt ggtcaaatgg atacgagaaa cgatatggtt tgttctatgt tgactttgag
     1441 acccaagaac gttatccaaa gaaaagtgcg tattggtata aaaaagtagc ggaaacacag
     1501 gttattgaat aaaactaata caaggatgcc tagctcaagt tagcatcctt gttttcatat
     1561 aagggaggca gtttttatgc tcgaattaaa aaatgagaac ttaacagtac aattttctga
     1621 attaggtggg caaattattt ctattaaaga caaggatggt atcgagtatc tttggcaagg
     1681 agatccgacc tattggagcg gtcaagcacc agttctattt cctatttgtg gaagtttacg
     1741 aaatgattgg gctatctatg aacctgcaga aagaccgaca tttacaggca caattccaag
     1801 gcatggactt gtgagaaaaa tgctgttcaa aaatgtaaaa aatactgaaa actctttaga
     1861 attttctatt tcatcaaacg aagaaaccgt aaaaaattac ccttttgaat ttgaactttc
     1921 tattaactac tcactatttg gcaaaacaat tcgaactgaa tatcggatga aaaaccttga
     1981 gggacataga aagctacctt actttattgg gggtcaccca ggttttaatt gtcccttgtt
     2041 ggatggtgag agttatgaag actattattt agaatttgaa aaagtagaat cttgtacagt
     2101 acctagaagt tttccagaaa ctggcttact tgatctgcga gatcgccggc cattcttgga
     2161 aaatcagaag accttggact tgtcctataa actgtttgag catgatgcca taacattgga
     2221 tcaattagca tcacgaaagg taaccttgaa atcaaaaaat catcagaaga aactttcgat
     2281 tgcatttgat gattttccat atttagtgat ttggtcaact acaaatcaga gtccctttat
     2341 cgcattagaa ccatggagcg gactctctac atcactggaa gaatctgaca tattcaatgc
     2401 caaacgaaat attagttacg ttgctccaaa agaagttgat acaaaatatt ttgatattat
//
LOCUS       DASFSW010000204         2450 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.205, whole genome shotgun sequence.
ACCESSION   DASFSW010000204 DASFSW010000000
VERSION     DASFSW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.205"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            253..1014
                     /locus_tag="U1099_002071"
     CDS             253..1014
                     /locus_tag="U1099_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM4868345.1"
                     /translation="MIKKERHERILDMLNIEGVITVKDMMKELNISDMTARRDLDALA
                     EAGFLTRIHGGAQRLYKDEEPHEKTHIEKKVLQTKEKKLIAQKANSIIKDGETIFIGP
                     GTTLEHLAVELKTRNIRVITNSLPVFLILNKSKTVDLLLIGGEYREITGAFVGSMATT
                     NLKTLRFSKAFVSANAVSDNAIATYSDVEGEIQQLALDNAVEKILLVDSTKFNRYDFF
                     NFYTLEQIDTIITDNQISQDQLNEFGQLTTIIQAN"
     gene            1030..1690
                     /locus_tag="U1099_002072"
                     /pseudo
     CDS             1030..1690
                     /locus_tag="U1099_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(1734..>2450)
                     /locus_tag="U1099_002073"
     CDS             complement(1734..>2450)
                     /locus_tag="U1099_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HEM4868346.1"
                     /translation="HEFEVEIAGHQIEKLVDEGVYIKVSFGVKQSGLIFIPNYQLDIL
                     EEENQTKYKVYIRETTSYFVYNQEHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERL
                     QEKITEINLLIELTETDKKYQDVKDDLVAEIAVLDIKLNQTNEKIATLNKMAEVLINL
                     KSEDPNSRKLARYDFSKLNLTESITVEQVTEEIRVLQEELGHYLDEYEGLARKLEIFV
                     KILNTNKQTEHEFQGDISLE"
BASE COUNT          802 a          413 c          366 g          869 t
ORIGIN      
        1 ttcctaaaac atctaaaatg gctaatttta ctgatgttcc acctaaatca acgcctatta
       61 tttctttcgt cataacttat tcctcctcag ttacactttc aatgacgttt acaaacatat
      121 aatataatca tgttttgtta tattgtcaac ttttttgtgt ttattgtgtt ttattttgtt
      181 tgtttttatc tttttgttat gataaaatac taacagatag ttattttata agtacagaaa
      241 ggaaatatag ttatgataaa gaaagaaaga cacgaacgta tcttagatat gttaaatatt
      301 gaaggtgtta ttactgtcaa agatatgatg aaagagctaa atatctctga tatgactgca
      361 agaagagatt tagatgcact tgctgaagca ggattcctga ctcgtataca tggaggagca
      421 caacgactct ataaagatga agaaccgcac gaaaaaactc atattgaaaa aaaggtactt
      481 caaactaaag agaagaaact tattgcacaa aaagccaatt caatcattaa agatggtgaa
      541 accattttta ttggtcctgg aacaactcta gaacatctag cagtcgagtt aaagactcgt
      601 aatattcgtg taattacaaa tagtcttcct gttttcctta tattgaataa gagtaaaact
      661 gttgacttat tattgattgg cggagagtac agagaaatta caggtgcttt cgttggttca
      721 atggctacaa ccaacttaaa aaccttacgt ttttctaaag catttgtaag tgctaacgcc
      781 gtatcagata acgctatcgc aacatacagt gatgttgaag gtgaaattca acaattagca
      841 ctggacaatg ctgttgaaaa aatcttgttg gttgatagta ccaaattcaa tcgttatgat
      901 ttctttaatt tctatactct agaacaaatt gataccatca tcaccgataa tcaaatatcc
      961 caagaccaat taaatgaatt tggccaacta acaactatta ttcaagctaa ttaaactaac
     1021 ttatgactta taaaaaatat tgtttttgat cttgatgata ccttgtatga tcatcttttg
     1081 ctattcaaaa atagtatcat acaatgtttt cctgaacttg atatttctga aattgaactt
     1141 atctataaac gattcaggta ttggtctgat attgcttatc caaaatacac taataagcaa
     1201 atttcaattg aagaactacg tatttttctt tgtaaacaga taataagtga atttggattt
     1261 tttagtattt ctgatgattt agcactctct ttccaaaaaa catacgaaaa agaactcagt
     1321 tcaattacct tgtttcctga attaaaagag attcttgaat actgttctgt aaaaaagatt
     1381 cctattggaa tcataacaaa tggtccagtt aaacaaaatt atcacaatta gacgttctaa
     1441 aatactttga taaagagaaa attatcatta gcaaagcaac aggtttccaa aaacctcaaa
     1501 tttcaatttt ctccccaatc aaacacttta tattagtgat aactttaaaa atgatattga
     1561 aggaagctta aatgctggtt ggaaatccat ttggtttaat cacaggaaac gcaaactacc
     1621 tattgatact aaaattcatg aagttcaaac tgctaaagac ttaaaagaac tagtctaccg
     1681 actgctttaa gttcaatata atattttttg accagtcaat agactggttt tgtttattct
     1741 agggaaatat ctccttgaaa ttcgtgttct gtctgtttat tagtattgag aatcttgaca
     1801 aatatttcca acttcctagc taatccttcg tactcatcaa gataatgtcc tagttcttct
     1861 tgcaacactc ttatttcctc ggttacttgt tctactgtaa ttgattctgt caaatttagt
     1921 tttgaaaaat cataccttgc taattttcga ctattcggat cttcactctt taagttgata
     1981 agcacttccg ccatcttatt caaggtggcg attttttcat tggtttgatt tagtttgata
     2041 tctagcactg ctatttctgc aaccaggtcg tctttaacgt cctggtattt tttgtctgtc
     2101 tcagttagct caatcaagag gttaatctca gtaatctttt cttgaagtct ttcaacagtc
     2161 ggtcgtctgt atggtattac tcgactatcg ttggttagct gtctaatcaa ggttcgtcct
     2221 ttgatatact gattcttatc cgaatgttct tggttataga caaagtatga ggttgtctca
     2281 cggatataga ctttatattt tgtctgattc tcttcttcaa gtatatctaa ttgatagttg
     2341 ggaatgaaaa taagaccgct ctgctttaca ccaaaagaca ccttgatata aacgccctca
     2401 tcaactaact tctctatttg gtgtcctgca atttccactt caaattcgtg
//
LOCUS       DASFSW010000205         2449 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.206, whole genome shotgun sequence.
ACCESSION   DASFSW010000205 DASFSW010000000
VERSION     DASFSW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.206"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1579)
                     /locus_tag="U1099_002074"
     CDS             complement(<1..1579)
                     /locus_tag="U1099_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868347.1"
                     /translation="MTQEQLLEMLRARLPRDQILLESFLRYQAVHFDEDWDSLIQNFT
                     TQRGIVTSPVQVVRFETEVSAFVEASPFDGVTDLSSYTQTFGQAGLKKLPQLNNDEKA
                     LVIEVALFNLATRFQLLDQEGAYQSISVAYLLDKSKAANLVNVYRVANNLSDRISRDI
                     EQFLLTYEPELESTQETLEEKKEVVEKIVIPESRQEITFREEGLVMIASLDEDELSQL
                     DLRTGQTEHLSAYEHLNLSQKFEILSHFDQVRNSRPKLPNLRLGEFDHEMEMTPIYED
                     NELLTYLEADGTAYDLQRPLTPQEEVILTEMGQTILAENIEKLTRLGIDLADVDEQQR
                     GILIDAAGRFHLKNADLALLGGYPKATVTQLALATELLQMGLTHDKTEFFLTSQLDLE
                     EARPIAYAFLHEDLTLEEARTFERDKLSQSDLSFRDWREQLSQTEPEIIMPQEKLPNP
                     IVEEALQRYPIDSRVTYKGQEFQVMAIEDSGVNNLIRIELQNDFTDVIEQNPVLFLRT
                     LEDITQALHVPSVEEKEE"
     gene            complement(1629..2180)
                     /locus_tag="U1099_002075"
     CDS             complement(1629..2180)
                     /locus_tag="U1099_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868348.1"
                     /translation="MAWNYDTISKTLSDMALENYEDMVKAFLAMELSIKNKSLLDTLY
                     QDFMGIDDLSLVSEDLRLRADAYQEQLQEEVTDLLDKLYRTGEGASFIMEVIASNNIS
                     DSLAQYEVLNEEDYSSLTLETLQDIIQKELSLTSQDYFGDVTYLALQKDLLDQKSHFL
                     QQYVTTLMDKLPQEKDQSALVLD"
     gene            complement(2164..2355)
                     /locus_tag="U1099_002076"
     CDS             complement(2164..2355)
                     /locus_tag="U1099_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEM4868349.1"
                     /translation="MEPKNIYTMDSDQDGLTDAQELALGTNPFSSDSDSDGLTDLEEV
                     QQGLNPIQQRKESSYGLEL"
     gene            complement(2356..>2449)
                     /locus_tag="U1099_002077"
     CDS             complement(2356..>2449)
                     /locus_tag="U1099_002077"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4868350.1"
                     /translation="GESQSLLALVGGGLLLGFAYGLSKRKMEDN"
BASE COUNT          652 a          529 c          491 g          777 t
ORIGIN      
        1 cttcctcttt ttcttcaaca gaaggaacat gaagagcctg agtgatgtct tctagtgtcc
       61 gtaaaaaaag aactggattt tgttcaatca catctgtgaa atcattttgt aattcaatcc
      121 gaattaggtt attaacgcct gagtcttcta tggccattac ttgaaactct tgccccttat
      181 aagttactct agaatcaatc gggtagcgtt gaagggcttc ctctacgata ggatttggta
      241 gtttttcttg aggcattatg atttcaggtt ctgtttgcga tagctgttct cgccagtctc
      301 taaaggatag gtctgattgg gacagtttgt ctctttcaaa ggttcttgct tcttcaagag
      361 tgaggtcttc atgcaagaag gcgtaggcaa tgggtcttgc ttcttctaag tctagctgac
      421 tagtcaagaa aaattctgtc ttgtcatgcg ttaatcccat ctgaagcaat tctgtcgcaa
      481 gggctaattg agtaacggtt gctttaggat agccccctaa taaggctagg tccgcattct
      541 tcaaatgaaa acgccctgca gcatctatca gtatcccacg ttgttgctca tcaacgtcag
      601 ctaagtctat ccctaaacga gtcagttttt cgatattttc agccaaaatg gtttgaccca
      661 tttcagttaa gatcacttct tcttgaggtg ttagaggacg ttgtaagtca taggctgtcc
      721 catcagcttc taaataggtt aatagctcat tgtcctcata gattggcgtc atttccattt
      781 catggtcaaa ctccccaagt ctcaggtttg gtagctttgg acggctattt cgcacttgat
      841 caaagtgact tagtatctca aatttttggg acaagtttag atgttcataa gccgataaat
      901 gttctgtttg ccctgttcgt aaatcgagtt gcgataactc gtcctcatca agacttgcaa
      961 tcatgacaag tccttcttca cgaaaggtga tctcttgacg agactctggt atgacaatct
     1021 tctcaacaac ctcctttttt tcctccaacg tttcctgtgt actttctaat tcaggttcat
     1081 aagtgagaag aaactgttca atatcacggc taatacggtc agacaaatta ttggccactc
     1141 gatagacatt aaccaaatta gcagctttac ttttatccag aagataagct accgaaatgg
     1201 actgataggc gccttcttgg tctaagagct gaaagcgagt ggctaggtta aagagggcta
     1261 cttcaatcac caaggctttc tcatcattgt ttaactgagg taacttcttt aatccagctt
     1321 gaccaaaggt ctgtgtgtag ctgcttagat ccgtcactcc atcaaagggg cttgcctcta
     1381 caaaagcaga aacttctgtt tcaaagcgaa ccacttgaac aggtgaggtg actattcctc
     1441 tttgggttgt aaaattttgg ataaggctat cccaatcctc atcaaaatgg actgcttgat
     1501 agcgtaaaaa ggattccaaa agaatctggt cacgtggcaa tcttgcccgc aacatttcaa
     1561 gtaattgttc ttgagtcata tattcctcca attaaaaagg ggctgagagg tattctcagc
     1621 ccactgcttt agtctaggac caaggcgctt tggtcttttt cttgtggtaa cttatccatt
     1681 aaagttgtca catactgttg taagaaatgg cttttctggt ctaacaaatc tttctgcaag
     1741 gcgagatagg tcacatctcc aaaataatct tgactcgtta aagacaactc cttttggata
     1801 atatcctgca aggtctctaa ggtgagagat gagtaatcct cctcattcaa aacctcatac
     1861 tgagcaaggg aatcagaaat gttgttggac gcaatcactt ccataatgaa actagctccc
     1921 tcacctgttc gatagagttt atccagaagg tccgtcactt cttcttgaag ctgttcctga
     1981 taagcgtctg ctcttaggcg caggtcttca ctgactaatg acaagtcatc aatgcccata
     2041 aagtcttgat agagcgtatc aagaagtgac ttgtttttga tactcaactc catggctaaa
     2101 aaggctttaa ccatgtcttc atagttttct agtgccatat cagagagagt ttttgaaatc
     2161 gtgtcataat tccaagccat aactgctctc cttacgctgt tgaattggat tcaagccttg
     2221 ttggacttct tctaggtctg tcagtccgtc actatccgaa tcactgctaa aaggattggt
     2281 gcctagagct aactcttgag catccgttaa gccatcttgg tctgaatcca ttgtgtaaat
     2341 gtttttgggt tccatttagt tgtcctccat tttgcgttta gaaagtccgt aagcaaagcc
     2401 aagaagaagt cctccaccga caagcgccaa gagggattga ctctctcct
//
LOCUS       DASFSW010000206         2447 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.207, whole genome shotgun sequence.
ACCESSION   DASFSW010000206 DASFSW010000000
VERSION     DASFSW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.207"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            6..821
                     /locus_tag="U1099_002078"
     CDS             6..821
                     /locus_tag="U1099_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM4868351.1"
                     /translation="MAKTKIYFWLKIDKKFFDNIFIKRLKTIPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFENLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRQNKERLDNVQKSLTNSNSCPTEIEIEKDIKKDIDINIELE
                     VDNKKTATDTNIYDYYQQRIGSLDGYQYEKLKDYLDIDKLEPELVKRAIDRGADNSKR
                     NFGYINKILKNWAQNGIRTIAQQDEEQRQFVDSKANKQKSNDWVPDPNYPPPY"
     gene            834..1292
                     /locus_tag="U1099_002079"
     CDS             834..1292
                     /locus_tag="U1099_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868352.1"
                     /translation="MKEQEFFEQAEKELEELNQHRADFMTMDFKELNNADYINFLNIG
                     NRIFSEDTTLNVYELYKHPDTRAKCFATIAKIAYHVNNMFQTEERMRTMIDSLELHFQ
                     NMVKKLVHQTDSDKLAELLLEIKKDNPNMTAEQESQFIRDIAVSGLLAMQ"
     gene            1370..1918
                     /locus_tag="U1099_002080"
     CDS             1370..1918
                     /locus_tag="U1099_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868353.1"
                     /translation="MTLNTFSDTANTFTFNYTFKDHDTAQVAGHALMGYMTGTFEQPG
                     IEVYYDNDKVGGDYNRLAVEYVADTELTETFRRICDSFQGYYNDPEAETDVEDQYRLE
                     RVEQLKQSETFDSLLEKVVIYELELLDYAERLLSDDPIPTDTEMAYMTLNLIGGKGVG
                     LFKSLDEDNEYSGLAYYNAEAE"
     gene            1968..2359
                     /locus_tag="U1099_002081"
                     /pseudo
     CDS             1968..2359
                     /locus_tag="U1099_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          906 a          413 c          542 g          586 t
ORIGIN      
        1 tgagaatggc aaaaactaaa atatattttt ggttgaagat tgataaaaaa ttttttgaca
       61 atattttcat caagagacta aagactattc caggcggtta cactatgaca gtaatctata
      121 tccgcctaat gcttgaaagt cttgaaagtg actgcattct ctactacgaa ggttattttg
      181 aaaatctcaa ggaagaattg gctttgaagt tggatgtgtc ggaagatgat atcgatatga
      241 ccatggcata ctttacaaaa tgcggtctga tacagattga tgaagataaa aatgcagagt
      301 taccacaggc caaagctatg gttatgagtg aaacaaactg ggctagctac aaacgggaac
      361 aacgacaaaa caaagagaga ttggacaatg tccaaaagtc tttgactaat tccaactcgt
      421 gtccaacaga gatagagata gagaaagata taaagaaaga tatagatata aatatagagt
      481 tagaagtaga taataaaaaa actgctactg acacaaatat ttatgattat tatcaacaaa
      541 gaattggttc cttggacggt tatcagtatg agaaactaaa agattactta gatattgata
      601 agcttgaacc tgaacttgtc aagagggcaa ttgatagagg agctgacaac tcaaaaagga
      661 actttggcta catcaataag attctaaaaa attgggcaca gaatggcatt cggacaatag
      721 ctcagcaaga cgaggagcag agacagtttg tcgatagtaa agcaaataaa caaaaatcaa
      781 acgattgggt gccagacccg aactatccac caccatacta gaaagaggcg caaatgaaag
      841 aacaagaatt ttttgagcaa gcagaaaagg agttagagga attaaaccag cacagagccg
      901 acttcatgac tatggatttt aaagaactca ataatgcaga ctatataaac tttttgaaca
      961 ttggaaaccg gattttttcc gaagatacca ctttgaacgt gtacgaacta tataagcatc
     1021 cagacacgag agcaaaatgc tttgcgacca ttgccaaaat tgcatatcac gttaacaaca
     1081 tgtttcagac agaagaacgt atgcgtacca tgattgatag tcttgaactg cattttcaaa
     1141 acatggttaa gaaactggta catcagacag atagtgacaa gctggctgag ctactactgg
     1201 aaatcaagaa agacaatccg aatatgacag cagaacagga aagccagttt atacgagata
     1261 ttgcagttag tggactatta gcaatgcagt aggaggcgac accgtgaccg atgataccga
     1321 aataaggaaa cttttccaaa tttatcaaac aactagggag aataaagaca tgacattaaa
     1381 cacattttca gacacagcaa acacatttac atttaactac acattcaaag accacgacac
     1441 cgcacaagtt gcagggcacg cgcttatggg ctacatgaca ggaacatttg aacaacctgg
     1501 gattgaagtg tattatgata atgataaagt gggcggagat tacaaccgtt tggcggtgga
     1561 atatgtggca gatactgaac ttactgaaac cttcaggcgg atttgtgaca gtttccaagg
     1621 ctactacaac gatcctgaag cagaaaccga cgttgaagac cagtaccgcc tggagcgcgt
     1681 ggaacaactc aagcagtcag aaacttttga tagcttgctt gaaaaggtgg tgatttatga
     1741 gctggagttg ctggactatg cggaacgttt gctaagtgat gaccctattc cgacagatac
     1801 ggaaatggct tatatgacac taaacttaat tggcggtaaa ggtgtagggc tattcaagtc
     1861 actggacgaa gataacgaat acagcgggct tgcttattac aacgctgagg cagaatagca
     1921 gagagaggca accgcctctt tttgtgctaa aattagagag gaataacatg atgaaaaata
     1981 aaggcggtag acctacaaaa atgacacaag gaacggtaaa gaaattagag gaagcatttc
     2041 taagagggct aagcgatgaa gaagcttgtt tgtatgcaaa ccaaccctgt atgattattg
     2101 caagaaaaac ccacagttta ctgaccgaaa agaactactt aagcaacgtg ttaaaacacg
     2161 agctaaacta aatatatcaa aagcgattga agacgggaat gtggacttgt caaaatggta
     2221 cctagaacgg aaagatgccg aatttaagac taagacaaaa cttgaacatg atggtatggt
     2281 atccgttgcg cctcataatc catttgaaga tttgacggtt gaagagttac gagcaatcat
     2341 tgctgaagat gcgggataaa tactattggt tgagagggtt atctaggtga agtacttcgg
     2401 caatttctaa aacgacgaat agacgaacgt atggaagcgc gtgatta
//
LOCUS       DASFSW010000207         2421 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.208, whole genome shotgun sequence.
ACCESSION   DASFSW010000207 DASFSW010000000
VERSION     DASFSW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.208"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            79..270
                     /locus_tag="U1099_002082"
     CDS             79..270
                     /locus_tag="U1099_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEM4868354.1"
                     /translation="MEPKNIYTRDSDQDGLTDAQELALGTNPFSSDTDSDGLSDLEEV
                     QQGLNPIQQRKERSYGLEL"
     gene            254..805
                     /locus_tag="U1099_002083"
     CDS             254..805
                     /locus_tag="U1099_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868355.1"
                     /translation="MAWNYDTISRTLSEMARENYEDMVKAFLAMELSIKNKSLLDTLY
                     QDFMGIDDLSLVSDDLRLRADGYQEQLQEEVTDLLDKLYRTGEGASFIMEVIASNNIS
                     ESLAQYEVLNEEDYSSLTLETLQDIIQKELSLTSQDYFGDVTYLALQKDLLDQKSHFL
                     QHYVTTLMDKLPQEKDQRDLVLD"
     gene            856..>2421
                     /locus_tag="U1099_002084"
     CDS             856..>2421
                     /locus_tag="U1099_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868356.1"
                     /translation="MTQEQLLEMLRARLPRDQILLESFLRYQAVHFDEDWDSLIQNFT
                     TQRGVVTSPVQVVRFETEVSAFVKASPFNEATDLSSYTQTFGQAGLKKLPQLNSDEKA
                     LVVEVALFNLATRFQLLDQEGAYQSISVASLLDKGKAANLVNVYRVANNLSDRISRDI
                     EQFLLTYEPELISTQETVEEREGAAEEKIVPELPQDITFREEGGIVIASLEEDLSQLD
                     LRTGQTEHLPAYEHLNLSQKFEILSHFDQVRNSRPKLPNLRRGEFDHEMEMTPIYEDN
                     ELLTYLEADGTVYDLQRPLTPQEEVILTEMGQTILAENTEKLTNLGIDLADVDEQQRG
                     ILIDAAGRFHLKNADLALLGGYPKATVTQLALATELLQMGLSHDKVEFFLTSQLDLED
                     ARPIAYAFLHEELTLEEARTFESAKQSQPDISFREWREHLSQTEPEIIVTQEKLPNPI
                     VEEALKRYPIASIVTYKGQEFQVMAIEDSGVNNLIRVELQNDFTDVIEQNPVLFLRTL
                     EDITQALHVPSVEE"
BASE COUNT          753 a          488 c          533 g          647 t
ORIGIN      
        1 cttttggcgc tcgttggtgg aggccttctt ttaggcctag cttacggact ttctaaacgc
       61 aaaatggagg acaactaaat ggaacctaaa aacatttaca caagagattc agaccaagat
      121 ggtctaacgg atgctcaaga gttagcttta gggaccaatc cttttagcag tgatacggat
      181 agtgacggac tgtcagacct tgaagaagtc caacaaggct tgaatccaat tcaacagcgt
      241 aaggagagaa gctatggctt ggaattatga tacaatttct agaacccttt ctgagatggc
      301 acgagaaaac tatgaagaca tggttaaggc ctttttagcc atggaattga gtatcaaaaa
      361 taagtcactt cttgatacgc tctatcaaga ctttatgggc attgatgact tatctttagt
      421 tagtgatgac ctgcgcctaa gagcagacgg ttatcaggaa cagcttcaag aagaagtgac
      481 ggacctcttg gataaactct atcgaacagg tgagggagct agtttcatta tggaagtgat
      541 tgcgtccaac aacatttctg agtcccttgc tcagtatgag gttttgaatg aggaggatta
      601 ctcgtctctc accttagaga ccttgcagga cattatccaa aaggagttgt ctttaacgag
      661 tcaagattat tttggagatg tgacctatct cgccttgcag aaggatttgt tagaccagaa
      721 aagccatttc ttacaacatt atgtgacaac tttgatggat aagttgccac aagaaaaaga
      781 ccaaagagac ttggtcctag actaaagcag tgggctgaga atacctctca gctccttttt
      841 taattggagg aacatatgac tcaagaacaa ttacttgaga tgttgcgggc gagattgcca
      901 cgtgaccaga ttcttttgga atccttttta cgctatcaag cagtccattt tgatgaggat
      961 tgggatagcc ttatccaaaa ttttacaact caaagaggag tagtcacctc acctgttcaa
     1021 gtggttcgct ttgaaacaga ggtttctgct tttgtcaagg cgagcccgtt taatgaagcg
     1081 acagacctaa gcagctacac acagaccttt ggtcaagctg gattaaagaa gttacctcaa
     1141 ttaaacagtg atgagaaagc cttggtggtt gaggtagccc tctttaacct agccactcgc
     1201 tttcagctct tagatcaaga aggcgcctat cagtccattt cggtagcttc tcttttggat
     1261 aaaggcaaag ctgctaattt ggtcaatgtc tatcgagtgg ccaataattt gtctgaccgt
     1321 attagccgtg atattgaaca gtttcttctc acttatgaac ctgaactaat aagcacccag
     1381 gaaacggtag aggaaagaga gggtgcagct gaagaaaaaa tagttccaga acttcctcaa
     1441 gacatcacct ttcgtgaaga agggggtatc gtcattgcaa gtcttgagga agatttatca
     1501 caactagatt tacgaacagg gcaaacagaa catttaccag cttatgaaca tctaaacttg
     1561 tcccaaaaat ttgagatatt aagtcacttt gaccaagtgc gaaatagccg tccaaaacta
     1621 ccaaacttga gacgtgggga gtttgaccat gaaatggaaa tgacaccaat ctatgaggac
     1681 aatgagctct taacctattt agaagctgat gggacagtct atgacttgca acgtcctcta
     1741 acacctcaag aagaagtaat cttaactgaa atgggtcaaa ccattttggc tgaaaatacc
     1801 gaaaaattga ccaatttagg gatagactta gctgacgttg acgagcagca acgtgggata
     1861 ctgatagatg ctgcagggcg ttttcatttg aagaatgcgg acctagcctt attagggggc
     1921 tatcctaaag caaccgttac tcaattagcc cttgcgacag aattgcttca gatgggattg
     1981 tcacacgata aagtagaatt tttcttgacc agtcagctag acttagaaga cgcaaggccc
     2041 attgcctacg ccttcttgca tgaagaactc actcttgaag aagctagaac ctttgaaagc
     2101 gccaaacagt cccaaccaga tatatctttt agagaatggc gagaacacct atcgcaaaca
     2161 gaacctgaaa tcattgtgac tcaagaaaaa ctaccaaatc ctatcgtaga agaagccctt
     2221 aaacgctatc cgattgcttc gattgtgact tataaggggc aagagtttca ggtaatggcc
     2281 attgaggact caggcgttaa taacctgata cgggttgaac tacaaaatga tttcacagat
     2341 gtgattgagc aaaatccagt tcttttctta cggacactag aggacatcac tcaggctctt
     2401 catgttcctt ctgtcgaaga a
//
LOCUS       DASFSW010000208         2418 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.209, whole genome shotgun sequence.
ACCESSION   DASFSW010000208 DASFSW010000000
VERSION     DASFSW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.209"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            250..843
                     /locus_tag="U1099_002085"
     CDS             250..843
                     /locus_tag="U1099_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868357.1"
                     /translation="MNGDLLKLAAKNFEPLLNKKITIELGRKGQKTVLDILFSKDHFF
                     HLAGLHKLNDIHFSHKKSSLVFDDILDDRINSDLLESSLYYDKKGVRSRLEILSYLYA
                     GFTKPNLVVRKAKNFPIKGSKLRWSYLVEFYIDDIRLGEFFIDNYRSGHSNEFIGVSI
                     FEKSEKDYTVNQTKFTILSIYETDIVSGNVVVLFTRM"
     gene            1085..>2418
                     /locus_tag="U1099_002086"
     CDS             1085..>2418
                     /locus_tag="U1099_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan-binding protein"
                     /protein_id="HEM4868358.1"
                     /translation="MTKQCHHHFLVNQEKAEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGQVAQADEVVTPSQGKTVQLTENPATNLPEAQPTPVVEQTNSLALTGQSDGAITVT
                     VPHDVVTQAVNQATAEGVTTIQDKPMDLGNTTSASDTSKQLDKAEVDAARQAETITQV
                     TNTYKADKATYEQDKTRVEQGNAALAASHKEATQVGKALNSSVDSTASKIKGQDQSAN
                     VTVTTQTVTSGDGSTVSGYQDYTSAVAAIDKQNKDNLSEYESKKKDADAFVAKNLVTQ
                     KENEAGLAKAKADNEAIDKRNQAGQKAVDDENKAGQAAVDAYNKDQQKLVTDREAEIA
                     SITKRNKEKEEAAKTENDAIDAYNAKEMERYKRDLAEISKGEEGYISQALAQALNLNN
                     GEPQARHSADTRNPNRIVAKGDAMLGGYSNILDSTGFFVYDTFKTGEILSFT"
BASE COUNT          802 a          415 c          511 g          690 t
ORIGIN      
        1 aataaatttt ttggaagaat acatttttag ataaatcttt atatgatgtt ttaattgaca
       61 ttaggaagta gttaggttat actatatgtg tagataacaa gagtatttac taagcgttaa
      121 gcattattgg tcggttggtt tgactggtag tgtttgcttt agtagggttg tgtgtgaaca
      181 cctcgagcca acaatgaaaa cactcgatga aaggattcgt atgaatcctt ttttcgtaga
      241 aagattacta tgaatggtga cttattaaaa ttagcagcaa aaaattttga acctttatta
      301 aataaaaaaa ttacaattga acttggtaga aaaggacaga aaacggtttt agatattctg
      361 ttttcaaaag accatttctt tcacttagct ggacttcata aactaaacga tatacatttt
      421 agccataaaa aatcttctct agtttttgat gatattttag acgaccggat taactctgac
      481 ttattagaat cttcgttgta ttatgataaa aaaggggtta ggtcacgttt agaaattttg
      541 tcgtatttat atgctggctt tacaaagcct aatcttgtcg ttagaaaagc aaagaatttc
      601 cccatcaaag gtagtaaatt aagatggagt tatttggtgg aattttacat tgatgacatc
      661 cgtctaggtg agttttttat tgataactat cgaagcggtc attctaatga atttattggt
      721 gttagtattt ttgaaaaatc tgaaaaagat tatactgtga atcaaacaaa gtttacaata
      781 ctttcaattt atgaaacaga tattgtgtca ggaaatgtag tggttctttt cactagaatg
      841 tagttctctt agttatatat aatatcatgt tgttatcgaa ttggggcgtg ctaatcacat
      901 ctgggagggt ctgatgggtc aggccccctt ttttataagc aattttcaga ggttatcggt
      961 agtcctaccg atagcttttt tctttgtcct tctttgatgt gataccaaaa tcaagacgtc
     1021 ttttttatga ttaggagaga gtgacagatt gttactactt atcaaaagaa aagaaggact
     1081 ttttatgacc aaacaatgtc atcatcactt tttagtcaat caggaaaaag cagaaaagca
     1141 tgtcttccgt aaaagtaaga agtaccgtac gctctgttcg gtagctcttg gaaccatggt
     1201 gacagctgtt gtcgcttggg gtggccaagt agcacaagct gacgaagtgg taacaccctc
     1261 gcaaggtaaa accgttcagc taacagaaaa tcctgctacc aacttaccag aagctcagcc
     1321 aactccagtt gttgaacaga cgaatagtct tgcgttaact ggtcaatcag atggggctat
     1381 cacggttacg gttcctcatg atgttgtgac acaggctgtc aatcaagcaa ctgctgaagg
     1441 ggttacgacc attcaggata agcctatgga tttgggaaac accacctctg cgagtgatac
     1501 cagcaagcaa ttagataaag cggaagtgga tgctgctagg caggctgaaa ccatcactca
     1561 ggtgaccaat acctataaag cggataaagc gacttatgaa caagataaaa cacgtgttga
     1621 acaagggaat gctgcgttgg ctgctagtca taaagaagcc actcaagtag gaaaggcctt
     1681 gaatagttca gtggatagca cagcttcaaa aatcaagggc caagatcagt cggctaatgt
     1741 gaccgttaca actcaaactg taacatcagg agatggttca acggtttcag gctatcagga
     1801 ttatacctct gcggtagctg caattgataa gcaaaataag gacaatctat ctgaatacga
     1861 atcaaagaaa aaagatgcag atgcctttgt cgcaaagaac ttagtgactc aaaaggaaaa
     1921 tgaagcgggt cttgctaaag caaaagctga taatgaagca attgataaac gcaatcaagc
     1981 cggacaaaaa gcagtggatg atgaaaacaa ggcaggtcaa gctgctgttg atgcctataa
     2041 taaagaccaa cagaaattag tcacagaccg tgaagctgag attgcttcta tcacgaaacg
     2101 caataaggaa aaagaagaag ccgccaagac agaaaatgac gccattgatg cctacaacgc
     2161 caaagaaatg gagcgctata aacgtgattt agctgagatt tcaaaaggtg aggaaggcta
     2221 catctcacaa gctcttgctc aggctctcaa tttgaataat ggagaaccac aagcccgtca
     2281 ttccgcggat acaagaaacc caaatcgtat cgttgctaag ggtgacgcta tgcttggtgg
     2341 gtattctaac atcctagatt cgacaggatt ctttgtctat gacaccttta aaacaggaga
     2401 aatcctttcc tttaccta
//
LOCUS       DASFSW010000209         2345 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.210, whole genome shotgun sequence.
ACCESSION   DASFSW010000209 DASFSW010000000
VERSION     DASFSW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.210"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..360
                     /locus_tag="U1099_002087"
     CDS             <1..360
                     /locus_tag="U1099_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4868359.1"
                     /translation="KEQKPKADEPKAEQPQMEAPKKDSEAPKSDKVETDKPMPETKQP
                     DMKQPKADKPEAEKAQMPRTEGMKPESKASMMPKAEAPKATLPNTGEASSAIGWLGGA
                     LATLATGLYLFKNKKEE"
     gene            796..>2345
                     /locus_tag="U1099_002088"
     CDS             796..>2345
                     /locus_tag="U1099_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEM4868360.1"
                     /translation="MSKQKVVTNLLLSTAVLGGFLICHVSSVSAETGNLQEIPTESAT
                     VSSENQTATAVDGISPTDNQLPTSEEGDEQAPEEKLVSSDSTREESATQVATDPDAPS
                     QSLSVSGAEISTADSNIQQAEEKLIQESNYHRFDTHQGEQIRKAVSIEKISADNDEIK
                     WKVTFDRKNWTFSGEGSGYYFILLKGLQLTKIIDSQSEEDIFNKFPKDVNSEANSGGQ
                     PYRFFSREKNANNDERSFESQWGWSVGRVGGELEKWKEESSSVYFLQVQRDSVAKEKA
                     TYELTAQVTNREMQTFPVLAVVKNFKERIFFSRDEITSAAGLRLKVSESAKPNNEGIT
                     IIGDSSPESDRTPEPRGDRTSDIVVPKADVPKVEQPKPKDPEVQLPAPKAPESVPPKV
                     MPPAPKVPDVKTPKEAPLPPKTPEVEVPKVMPPTPKVPDVKTPKVEYPDTDAPVIESP
                     KAPETERPEVLPPTPKVPEVEVPKVIPPTSKVPEAELPKVEQPKPKVPEVHLPAPKSP
                     ETEPPKVMS"
BASE COUNT          835 a          446 c          512 g          552 t
ORIGIN      
        1 aaggaacaaa aaccgaaagc cgacgagcca aaggcagagc aaccacaaat ggaagctcct
       61 aagaaagatt cggaagcacc aaaatctgat aaagtagaaa ctgacaaacc aatgccagaa
      121 actaaacaac cagatatgaa gcaaccgaag gcagataaac cagaagcaga gaaagctcaa
      181 atgccacgga cagagggtat gaagcctgaa tctaaggctt caatgatgcc aaaagcagag
      241 gcaccaaaag caactttgcc aaatacaggc gaagcaagta gcgcaatagg ctggctaggt
      301 ggtgctttag ccactcttgc cacaggcttg tatctattca aaaacaaaaa agaagaatag
      361 tcattattga ataagatatg agaacttccc tcttctaagt tcataaaaga ctccagatca
      421 tcgctgtaga agccttggtt tggagtcttt ttgttaaata ttcttagaaa ataacaaaat
      481 agataaatat tagataccct ctagaaaaaa tttagtttag gatgttatac taacgctaaa
      541 acaataatat caatttttga aaatactaga ttggaataaa aatctaatcg ccatatttta
      601 gaacctgtac ttacaagtaa agagctttta tgtaagagat agtttttcgc taatcaagtt
      661 aattttttga gggaatgctg gctgtatttt gaaaaaaacg aacgatgagt agcttaaaaa
      721 tgccttagat gggagtgcta acctgtaaat acaggttctt agtaaattga agtaaataga
      781 gtaaaggagc acattatgag caaacaaaaa gttgtgacaa atctattgct tagtacagct
      841 gtactaggtg gttttctaat atgtcacgta tcatctgtaa gcgcagaaac aggcaatctt
      901 caagaaatac ctactgaatc tgctacggtt agctcagaaa atcaaactgc gaccgcagtc
      961 gatggaattt cacctacgga taaccaatta cctacgtcag aagaaggtga tgaacaggct
     1021 ccagaagaaa aactagtttc tagcgattct acgagagaag aaagtgctac gcaggtagcg
     1081 acggatccgg atgcgccatc tcaatcatta agtgtgtcag gggcggaaat ttcaacggca
     1141 gattcaaata ttcaacaagc cgaagagaaa ctgattcaag aatcgaatta tcatagattt
     1201 gacactcatc aaggtgaaca gattaggaag gcagtatcta tagaaaaaat atcggcagat
     1261 aatgacgaaa ttaagtggaa agtaactttt gatagaaaaa attggacctt tagtggagag
     1321 gggagtggct attactttat tcttctaaaa ggcttacaat taaccaaaat tattgatagc
     1381 cagtctgaag aggatatttt caataagttc cctaaggatg tcaatagtga ggcaaattct
     1441 ggcgggcagc cgtatcgctt ttttagtaga gaaaaaaatg ctaataatga tgagcgtagt
     1501 tttgaaagtc agtggggctg gtctgttgga cgtgtaggag gagagctaga aaaatggaag
     1561 gaagaaagtt ccagcgtcta ttttctacaa gtacagcgag attccgttgc taaagaaaaa
     1621 gcgacatatg aactgacggc acaggtgaca aatcgagaaa tgcagacttt cccggtttta
     1681 gctgtggtga agaattttaa agagcgaata tttttctcca gagatgagat tacttctgct
     1741 gctggtttga gattaaaggt atcggaaagt gctaagccaa ataatgaagg gattactatt
     1801 atcggagatt catctccaga aagcgacagg actcctgaac cgcggggaga taggacttcg
     1861 gatatagttg taccaaaggc agatgttcca aaagttgagc aaccaaaacc aaaggaccct
     1921 gaagttcaac tgcctgctcc taaggctccg gaatctgtgc caccaaaagt aatgcctcca
     1981 gcacccaaag ttccagacgt taagacgccc aaagaggcgc ctctgccacc aaaaactccg
     2041 gaagttgagg ttccaaaagt aatgcctccg acaccgaagg ttccagatgt taagacgcca
     2101 aaagttgagt accctgatac agatgctcct gtgattgagt caccaaaagc tcctgaaact
     2161 gaacgaccag aagtcttacc accgacgcca aaagtcccag aagttgaggt tccgaaagta
     2221 attccaccga cctcaaaagt tccagaagct gagctaccga aagttgagca gccaaaaccg
     2281 aaggttccag aagttcacct tcctgctcct aagtctccgg aaactgagcc acccaaagta
     2341 atgtc
//
LOCUS       DASFSW010000210         2312 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.211, whole genome shotgun sequence.
ACCESSION   DASFSW010000210 DASFSW010000000
VERSION     DASFSW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.211"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(258..1100)
                     /locus_tag="U1099_002089"
     CDS             complement(258..1100)
                     /locus_tag="U1099_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM4868361.1"
                     /translation="MTRYTFSNGVTVPKIGFGTWQIPDGEVAYGAVSYALEAGYRHID
                     TAQIYGNEASVGRAIADSGLAREDIFLTTKVWNDKIGYEDTLASVEESMQKLQVDYLD
                     LLLIHWPNPKASRETIGWKKRNAQVWKALEELYRAGKVKVIGVSNFMEHHLEALLETA
                     EIIPMANQIMLAPGTPQSELVAYCKAKGILLEAYSPFGTGSLFQSQEAADLAKEAGCS
                     VAQLALAWSLDKGFLPLPKSTSPENIKANLEIDGLAISPTAVAKLDKLEGVKGRLDPD
                     QAEF"
     gene            complement(1216..1731)
                     /locus_tag="U1099_002090"
     CDS             complement(1216..1731)
                     /locus_tag="U1099_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEM4868362.1"
                     /translation="MNTSKWTARDMAEIALVAAIYVALTLTPPLNAISFGAVQFRLSE
                     MLNFLAFYNRKYIIAVTIGCMISNLIGFGVIDLFVGGFSTLIFVTLGVILFEKYKKDY
                     LFGGLFNKAFFYFSFFFAVTMFTIALELKVLYDLPFFLTWLTTAAGELLSLLVGAVVI
                     DKLSKKINFEK"
     regulatory      complement(1729..1831)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            1860..2297
                     /locus_tag="U1099_002091"
     CDS             1860..2297
                     /locus_tag="U1099_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEM4868363.1"
                     /translation="MKKLFYQLINNEVILYLIAGVAATLVYMVTRMGLFLVIPSILLV
                     TLLANAVAILFAFWTNDRFVFKQAREGWPQRLVKFVSARIATLVMDMALAYLLVQAYP
                     QLIGQFVNHDLTLVNAISTLFSQVLVIVLNYVLSKLFVFKDKK"
BASE COUNT          743 a          489 c          379 g          701 t
ORIGIN      
        1 tatctatata tgcttatttt tataactttc ttgtgatata ttttgttgtg ataatatttt
       61 ttgtctttac ccaaaataca gatttaagtt gaaacgatgt gatgtttatt acgcaaaaaa
      121 cacgatgccg cgagaaaaat ctactttttt acataaaata atatctaata tatatttcga
      181 aatacatact acttccatta taattagtct agaaacaaaa ataaggctgg acatatccaa
      241 ccttatattc atatttttta aaattcagcc tgatccgggt caagtcgacc ttttactcct
      301 tctagtttat ccaactttgc aactgctgtc ggactaatag ccaagccatc aatttccaaa
      361 ttcgccttaa tattttctgg actagttgat ttcgggagag ggagaaagcc tttatctaaa
      421 gaccaagcta gtgccagctg agcaacacta caaccagctt ctttagccaa atcggctgcc
      481 tcttggcttt ggaaaaggct tcctgtacca aatgggctat aggcctccaa gagaatccct
      541 ttggctttgc agtaagcaac taattctgac tgaggagttc ctggagctaa cataatctga
      601 ttagccatcg ggataatctc agccgtttcc agcaaagctt ctaggtgatg ttccataaag
      661 ttagaaactc caattacttt cacttttcca gcacgataga gctcttctaa tgccttccaa
      721 acctgggcat tgcgtttttt ccatccaatt gtctcacgac tagcctttgg atttggccaa
      781 tggatcagta acaaatccag ataatccacc tgaagttttt gcatggattc ttctactgaa
      841 gcaagggtat cttcatagcc aatcttatca ttccaaacct tagttgttaa aaaaatgtcc
      901 tcacgagcta ggccactatc agctatagcc cgaccaacac tagcttcatt gccataaatt
      961 tgagcagtat caatatgacg ataaccagct tccaatgcat aagaaaccgc accgtacgcc
     1021 acttcaccat cgggtatctg ccaagttcca aaaccaattt taggaacagt tactccattt
     1081 gaaaacgtat atcttgtcat aaaagacctc ctatttcttc tatccccagt ataaactaaa
     1141 ttgtcaatca atacaaaata atgcgaccta aaaataaaag acacccgaaa gtgtctgctc
     1201 acatttattg tatacttact tttcaaaatt tattttcttg gatagtttat cgataacaac
     1261 agctccaact aatagagaaa gcaattctcc tgctgctgtt gtcaaccaag ttagaaagaa
     1321 tggtaaatca tataagactt ttaattctaa agcaatggta aacatagtta ccgcaaaaaa
     1381 gaatgagaaa taaaagaaag ccttgttaaa taatccaccg aatagatagt ccttcttgta
     1441 tttttcaaac aggataactc ccaacgtaac gaaaattaat gtcgagaatc cacccacaaa
     1501 gaggtcaatc acaccaaaac caatcaagtt ggaaatcatg cagccaatgg tcacagcgat
     1561 aatatactta cgattataaa aagccaagaa attgagcatt tcagacaaac gaaactgaac
     1621 agccccgaag gaaatagcat tcaatggcgg tgtcaaggta agagcgacat aaatcgccgc
     1681 aacaagagca atttctgcca tatctctggc agtccattta gatgtattca taaaattctc
     1741 ctttagcaaa aaagcttaaa atatgcttgg gtaaagtgag caagtcccaa gggtcattga
     1801 atttgtcaac aacgttgatt agtatagcat atttttgcta aaaatgatag actagtccta
     1861 tgaaaaaact attttatcaa ctcatcaata acgaagttat tctctatttg attgcaggtg
     1921 tggcagcaac ccttgtctat atggtcactc gaatggggct atttttagtc attccttcca
     1981 ttctattggt tactctttta gccaatgcag tagctatcct ctttgccttt tggaccaacg
     2041 accgtttcgt tttcaaacaa gccagagagg gttggcctca gcgattggtc aagtttgtat
     2101 ctgcccgcat cgcgaccttg gtcatggata tggctctagc ctatttatta gtacaagcct
     2161 acccacaatt gattgggcag tttgtcaacc atgatttgac acttgtcaac gccatttcca
     2221 ctctcttctc tcaagtcttg gtcattgtcc taaactacgt tttaagtaaa ttatttgtat
     2281 ttaaagataa gaaataaggt tgggcaaaaa ga
//
LOCUS       DASFSW010000211         2306 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.212, whole genome shotgun sequence.
ACCESSION   DASFSW010000211 DASFSW010000000
VERSION     DASFSW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.212"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(101..658)
                     /locus_tag="U1099_002092"
     CDS             complement(101..658)
                     /locus_tag="U1099_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4868364.1"
                     /translation="MEIRLAHPNEMEAIVDILEDARAFLATSGINQWQGEYPNKENIF
                     DDILSGRGYVGIVDGKVAAYAAVHRGQEAEYEAIYEGKWQHNHPVYTTFHRIAAADEF
                     RGQGIIQTFLQGLIEGQKGPDFRCDTHEQNKTMQHILKKLGYVYCGKVPIDGERLAYQ
                     KIKQKSERSLYQEISEDDRWLLGNN"
     gene            complement(658..1749)
                     /gene="serC"
                     /locus_tag="U1099_002093"
     CDS             complement(658..1749)
                     /gene="serC"
                     /locus_tag="U1099_002093"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEM4868365.1"
                     /translation="MTIYNFSAGPAVLPKPVLERAQAEFLDYNGSGMSVLEMSHRSKD
                     FDDIIKGAEATLRELMAIPDNYKVIFLQGGASLEFTMIPLNFAQGKKAYYLVGGSWGK
                     KAYTEAVKLSKTIAFEPILLGSTEDITYAELPTFDKNDIDPTAAYVHLTTNNTIEGTT
                     VYGIPDTNGVPVIADMSSNILAARYNVEDFAMIYAGAQKNIGPAGVTVVIVREDFLND
                     QPMLSSMLDYRIQAENDSLYNTPPAYSIYISKLVFEWVKEIGGVDEMEKINREKSGLL
                     YDYIDQSNFYTNPVRKKEERSVANIPFVSPSEELDAKFVKEATTAGFKNIKGHRSVGG
                     MRASLYNAFPHQGVVELIEFMKKFAAENA"
     gene            complement(2219..>2306)
                     /locus_tag="U1099_002094"
     CDS             complement(2219..>2306)
                     /locus_tag="U1099_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEM4868366.1"
                     /translation="KPNQAIKEVAKRYQLKKQEVYDIYHGLG"
BASE COUNT          659 a          482 c          427 g          738 t
ORIGIN      
        1 ctggactgtt aaacccaatc aaccactgtg ctgagatgtt gactcgaacc ttgagaagtg
       61 atatgaagct ttttcccggt ccttttcttt ctttatttca ttaattattt ccgagtaacc
      121 aacggtcgtc ttcactgatt tcctggtaaa gactccgctc gctcttttgc ttgatttttt
      181 gataggctag acgttctcca tctattggga ctttaccaca atagacatag cctaattttt
      241 ttagaatgtg ctgcattgtc ttattctgct cgtgggtatc acagcggaaa tccggtcctt
      301 tttgaccttc aattaaacct tgcaaaaaag tttgtatgat tccttgacca cgaaattcgt
      361 cagctgctgc aattctatga aatgttgtat atacaggatg attgtgctgc cattttcctt
      421 catatatggc ctcatattct gcttcttgac cacgatgaac ggcggcatag gctgccacct
      481 taccgtcaac tataccaacg taacctctgc cagaaagaat gtcatcaaag atattctctt
      541 tattaggata ctctccctgc cattgattga taccagaggt tgctagaaat gcacgagcat
      601 cttcaagaat atcgacaata gcctccatct catttggatg tgctaatcta atttccatta
      661 agcattctcc gctgcaaatt ttttcatgaa ttcaatcaat tcaaccacac cttgatgtgg
      721 gaaagcatta tagagcgacg cccgcatccc acctactgaa cggtgacctt tgatgttttt
      781 aaaaccagct gttgttgctt ctttgacaaa cttggcatct aattcttcac ttggtgagac
      841 aaaaggaatg ttcgctactg aacgctcttc ttttttgcga acagggtttg tatagaaatt
      901 agactggtcg atatagtcat aaagcaaacc agatttttca cggttgattt tttccatctc
      961 atcaacacca ccaatttctt ttacccattc aaagacgagt ttagaaatat agatagaata
     1021 agctggtggc gtattataaa gagagtcatt ttctgcttga atacggtagt ccaacatgct
     1081 tgaaagcatt ggttgatcat tgaggaagtc ctcacgaaca atcaccaccg tcacaccagc
     1141 aggaccaata tttttctgag cgccagcata aatcatggca aaatcttcaa cattatagcg
     1201 agctgctaag atgtttgaag acatgtcggc aatgactggc acaccattgg tgtcaggaat
     1261 accataaact gttgttcctt caatagtgtt atttgttgtc aaatgcacgt aagcagcagt
     1321 tggatcaata tcatttttat cgaaggttgg caattctgca taggtaatat cttccgttga
     1381 tccaagcaaa attggttcaa aggcaattgt tttagacagc ttcacagcct cagtataggc
     1441 ttttttaccc catgagccac ctacaagata gtaggctttc ttaccttgag cgaagttgag
     1501 aggaatcatt gtgaattcca aagaagctcc accttgcaag aaaataacct tgtaattatc
     1561 tggaatggcc atcaattcac gaagcgttgc ctcagcacct ttaataatat catcgaagtc
     1621 cttggagcga tgcgacatct ccaaaacgct cattcccgaa ccattgtagt ccaagaattc
     1681 agcttgggcg cgttcaagta ctggttttgg caatactgca ggacctgcag agaagttgta
     1741 gattgtcata aaatccctcc attaagttag tctgttcatt atatcacaaa tattcagaca
     1801 attccataaa atatgattat tttgtaaaaa cattcggatt tcttatattt attctacttt
     1861 ctaaagaacc tcttactttt agccataaac gaggcgcttg cctcacaggg attcatcagg
     1921 tttcaatgaa aattttatga ccagatttga ctgtttgggg agtgtcgcca attgctatct
     1981 gcatattaat actcaatgaa aatcaaaagt agcttagctg ggatatagtt gaaggctgga
     2041 agtatagaga actgtgttcg cgtcattaag ccgaagatag aactctgttc ctttctcatt
     2101 tcaaggtaac agactgaaaa gatccactgg agcttttcac tcatcaaggc tcttgacaac
     2161 ggataatttt gattttcgaa gagtataagc ttgcataaac atacaaaaaa actaggtctt
     2221 aacctagtcc atgatatata tcgtatactt cttgtttttt gagttggtag cgtttggcca
     2281 cttctttgat agcctgatta ggtttt
//
LOCUS       DASFSW010000212         2304 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.213, whole genome shotgun sequence.
ACCESSION   DASFSW010000212 DASFSW010000000
VERSION     DASFSW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.213"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1060
                     /locus_tag="U1099_002095"
     CDS             <1..1060
                     /locus_tag="U1099_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEM4868367.1"
                     /translation="FVACTRENSAKSGITGEVFEHNGATCFNIDVPNIGSAKAILYDI
                     MALKKSIEIAKDRNDTSPIFYILACRIGPFIYLFKKQIESIGGQLFVNPDGHEWLREK
                     WSYPVRQYWKFSESLMLKYADLLICDSKNIEKYIHEDYRKYAPETSYIAYGTDLDKSR
                     LSPTDSVVREWYKEKEISENDYYLVVGRFVPENNYEVMIREFMKSYSRKDFVLITNVE
                     HNSFYEKLKKETGFDKDKRIKFVGTVYNQELLKYIRENAFAYFHGHEVGGTNPSLLEA
                     LSSTKLNLLLDVGFNREVGEEGAKYWNKDNLHRVIDSCEQLSQEQINDMDSLSTKQVK
                     ERFSWDFIVDEYEKLFKG"
     gene            1064..2221
                     /locus_tag="U1099_002096"
     CDS             1064..2221
                     /locus_tag="U1099_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM4868368.1"
                     /translation="MKKILYLHAGAELYGADKVLLELIKGLDKNEFEAHVILPNDGVL
                     VPALREVGAQVEVINYPILRRKYFNPKGIFDYFISYHHYSKQIAQYAIENKVDIIHNN
                     TTAVLEGIYLKRKLKLPLLWHVHEIIVKPKFISDSINFLMGRFADKIVTVSQAVANHI
                     KQSPHIKDDQISVIYNGVDNKVYYQSDARSVRERFDIDEEALVIGMVGRVNAWKGQGD
                     FLEAVAPILEQNPKAIAFIAGSAFEGEEWRVVELEKKISQLKVSSQVRRIDYYANTTE
                     LYNMFDIFVLPSTNPDPLPTVVLEAMACGKPVVGYRHGGVCEMVKEGVNGFLVTPNSP
                     LNLSKVILQLSENINLRKKIGNNSIERQKEHFSLKSYVKNFSKVYTSLKVY"
BASE COUNT          789 a          334 c          454 g          727 t
ORIGIN      
        1 ttttgttgca tgtacaagag aaaattcagc aaaatcaggt attacaggag aagtttttga
       61 acataatgga gcaacatgtt ttaatattga tgtgccaaat attggttcag caaaagccat
      121 tctttatgat attatggctc tcaagaaatc tattgaaatt gccaaagata gaaatgatac
      181 ctctccaatt ttctacattc ttgcttgtcg gattggtcct ttcatttatc tttttaagaa
      241 gcagattgaa tcaattggag gtcaactttt cgtaaaccca gacggtcatg aatggctacg
      301 tgaaaagtgg agttatcccg tccgacagta ttggaaattt tctgagagtt tgatgttaaa
      361 atacgctgat ttactaattt gtgatagcaa aaacattgaa aaatatattc atgaagatta
      421 tcgaaaatat gctcctgaaa catcttatat tgcttatgga acagacttag ataaatcacg
      481 cctttctccg acagatagtg tagtacgtga gtggtataag gagaaggaaa tttcagaaaa
      541 tgattactat ttggttgttg gacgatttgt gcctgaaaat aactatgaag taatgattcg
      601 agagtttatg aaatcatatt caagaaaaga ttttgttttg ataacgaatg tagagcataa
      661 ttccttttat gagaaattga aaaaagaaac agggttcgat aaagataagc gtataaagtt
      721 tgttggaaca gtctataatc aggagctgtt aaaatatatt cgtgaaaatg catttgctta
      781 ttttcatggt cacgaggttg gaggaacgaa cccatcttta cttgaagcac tttcttctac
      841 taaactaaat cttcttctag atgtgggctt taatagagaa gtaggggaag aaggagcgaa
      901 atactggaat aaagataatc ttcacagagt tattgacagt tgtgagcaat tatcacaaga
      961 acaaattaat gatatggata gtttatcaac aaaacaagtc aaagaaagat tttcttggga
     1021 ttttattgtt gatgagtatg agaagttgtt taaaggataa gttatgaaaa agattctata
     1081 tctccatgct ggagcagaat tatatggggc agataaggtt ctcttggaac ttataaaagg
     1141 cttagataag aatgaatttg aagcgcatgt tatcctacct aatgatggag tcctagtgcc
     1201 agcattaaga gaagttggtg cgcaagttga agttattaac tatccaattc tacgtaggaa
     1261 atattttaat ccaaaaggga tttttgacta cttcatatca tatcatcact attctaaaca
     1321 gattgctcaa tatgccatag aaaataaggt tgacataatt cacaataata ctaccgctgt
     1381 cttagaaggc atttatctga agcgaaaact caaattacct ttgttgtggc atgttcatga
     1441 gattattgtc aaacctaaat tcatctctga ttcgatcaat tttttaatgg ggcgttttgc
     1501 tgataagatt gtgacagttt cacaggctgt ggcaaaccat ataaaacaat cacctcatat
     1561 caaagatgac caaatcagtg taatctacaa tggggtagat aataaagtgt attatcagtc
     1621 cgatgctcgg tctgttcgag aaagatttga cattgacgaa gaggctcttg tcattggtat
     1681 ggtcggtcga gtcaatgcgt ggaaaggaca aggagatttt ttagaagcag ttgctcctat
     1741 actcgaacag aatccaaaag ctatcgcctt tatagcagga agtgcttttg aaggagaaga
     1801 gtggcgagta gtagaattag aaaagaagat ttctcaatta aaggtctctt ctcaagtcag
     1861 acgaattgat tattatgcaa ataccactga attatataat atgtttgata tttttgtact
     1921 tccaagtact aatccagacc ctctaccaac ggttgtacta gaagcaatgg catgcggtaa
     1981 acctgttgtc ggttaccgac atggtggtgt ttgtgagatg gtgaaagaag gtgttaacgg
     2041 tttcttagtc actccgaact caccgttaaa tttatcaaaa gtaattcttc agttatcgga
     2101 aaatataaat ctcagaaaaa aaattggtaa taattctata gaacgtcaaa aagaacattt
     2161 ttctttaaaa agctatgtaa aaaatttttc gaaagtctac acctccctca aagtatactg
     2221 attggctgaa gtgaatgctt tagtatagcg atttatcgta ttatcattcg ataaaacaaa
     2281 tgttcagaaa cagttataag ttat
//
LOCUS       DASFSW010000213         2302 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.214, whole genome shotgun sequence.
ACCESSION   DASFSW010000213 DASFSW010000000
VERSION     DASFSW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.214"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>2302)
                     /locus_tag="U1099_002097"
     CDS             complement(<1..>2302)
                     /locus_tag="U1099_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4868369.1"
                     /translation="YPLTVDVFSPNRAKTTSLTFEDYLPAGYAFDKEKTQAENRNYTL
                     SFDAAKNFVTLTAKDALLQEINQDLTKSYQLMAPKLYGSLQNDGATYSNSYQLLINKG
                     TPNAYTVTSNVVTVRTPGDGTTTSRIEPQKKNDNEDGVLINDTVVALGTTNHYRLTWD
                     LDQYKGDRTSKETIARGFFFVDDYPEEVLDLVDNGTGITTPDGKTVSGITVKAYASLS
                     EAPKDLQDKLAHAKISPKGAFQVFMPNDNQAFYDQYVKTGTSLNLLTKMTVRDSLYGQ
                     TKTYRNKAYQVDFGNGYETDEVTNTLVSPSPKKQNLNKDKVDINGKPMVVGSQNYYTL
                     SWDLDQYRGIKADKTQIAKGFYFVDDYPEEALLPDEASIQLVTTDGKAVSGVTVKTYT
                     SLSEAPKPLQVALSKRKIAPKGAFQVFMAEDPQAFYDSYVTQGQNITIVTPMTVRESM
                     LNSGKSYDNVAYQVDFGQAYETNTVTNHVPKVNPHKSNTNIKGVSIDGKTVLPNTINY
                     YKIVLDYSQYKDLVVTDDVLTKGFYMVDDYPEEALILNSDGVQVMDKAGNLVKGISVK
                     AYASLSEAPKVVQDAMAKRQFTPKGAIQVLSADDPKAFYEAYVKTGQTLVVPLPMTIK
                     NELTKTGGKYENTAYQIDFGLAYVTETVVNNVPQLDPQKDVVIDLSQKEKSLDGKEVA
                     LNQVFNYRLVGALIPGNRATPLIEYRFDDDYDESHDDYNGVYKAYTLVDVTLKDGTVL
                     PKGTEVTKYTLQQVDTSKGTVTISFDQ"
BASE COUNT          601 a          490 c          475 g          736 t
ORIGIN      
        1 ctgatcaaaa ctgatggtaa ccgttccttt tgacgtgtcg acctgttgta gcgtgtactt
       61 agtcacttct gttccttttg gtaaaaccgt tccatctttt agagtcacat ctactaaggt
      121 ataagccttg taaacaccat tatagtcgtc atggctttca tcgtaatcat cgtcaaagcg
      181 gtactcgatg agtggtgtcg cacgattacc aggaataagt gctcccacca agcggtagtt
      241 aaagacctga ttcaaagcaa cttcttttcc atcaagactt ttctcctttt gtgacaaatc
      301 aatcaccaca tctttttgtg ggtctagttg tggcacatta ttaaccacag tctctgtcac
      361 ataagccaaa ccaaagtcaa tctgataagc ggtgttttca tacttcccac cagtttttgt
      421 cagctcattc ttaatagtca ttggaagggg caccactaag gtttgaccag ttttaacata
      481 agcctcataa aatgcctttg gatcatcagc gcttaaaact tgaatggccc ctttaggtgt
      541 gaactggcgt ttggccatag cgtcttgaac cactttaggc gcttctgaca atgaggcata
      601 ggctttcacc gaaatccctt tgacaagatt tcctgcctta tccatcactt gaacgccatc
      661 tgagtttagg ataagcgctt cttcagggta atcatccacc atgtaaaaac cttttgtaag
      721 aacatcatct gtcactacca aatccttgta ctgactgtaa tccagaacaa ttttgtagta
      781 attaatggta tttggaagaa cggtcttccc atcaatagac acgcccttta tattggtatt
      841 ggacttatga gggtttactt ttggcacgtg attcgttacc gtattggtct cataggcttg
      901 tccaaagtct acttgataag ctacattgtc ataagacttt cctgaattaa gcatagactc
      961 acggacagtc ataggagtaa cgatggtgat attttgacct tgcgttacat aagaatcata
     1021 gaaggcttgc ggatcttctg ccataaagac ttggaaggct cctttaggcg caatcttgcg
     1081 ttttgaaaga gctacttgta ggggtttagg ggcttctgat aagctcgtat aagtttttac
     1141 cgtcacacct gacactgctt tgccgtcagt tgtcacaagt tgaatacttg cttcatctgg
     1201 taatagagct tcttcgggat aatcatccac aaagtagaaa ccttttgcaa tttgagtctt
     1261 atcagcctta atgcctcggt attggtccaa gtcccatgac aaggtatagt agttttgtga
     1321 acccactacc atcggttttc cgttaatgtc caccttatcc ttattcaagt tttgtttctt
     1381 aggtgatggg cttacaaggg tattcgtcac ctcatcggtt tcatagccat tgccaaaatc
     1441 cacttggtag gctttgtttc gataagtctt cgtctgacca taaagactat ctctgaccgt
     1501 cattttggtg agaaggttca aagaggttcc tgttttaaca tactggtcat aaaaagcctg
     1561 attgtcatta ggcataaaga cttggaaagc cccttttgga gaaatcttag catgagcgag
     1621 tttatcttga aggtcttttg gagcttctga cagcgacgca taagccttga cagtaatccc
     1681 tgatacggtt ttgccatcag gagtggtgat acctgttcca ttgtctacta aatccaacac
     1741 ttcttctggg tagtcatcta caaagaaaaa accacgagca attgtctctt tagaagttct
     1801 atcgccttta tattgatcta aatcccaggt caaacggtaa tgattagttg ttcctaaagc
     1861 cactaccgta tcgttaatga gcacaccatc ttcattgtca tttttcttct gcggctcaat
     1921 acggctagtg gttgttccat caccaggcgt acgaaccgtc acgacattgg aagtcactgt
     1981 ataggcattt ggggttccct tattgatgag gagctgataa ctgttggaat aggttgcccc
     2041 atcattttga aggctaccat aaagtttcgg agccataagt tggtaagact tggtgaggtc
     2101 ttgattgatc tcttgaagta aggcatcctt ggcggtcaag gtcacaaagt tcttagcagc
     2161 atcaaagctg agggtataat ttctattttc tgcctgcgtt ttttccttgt caaaggcata
     2221 gcctgctgga aggtaatctt caaatgtaag gctagtggtt ttggctcgat ttggagaaaa
     2281 aacgtctacg gttaaagggt aa
//
LOCUS       DASFSW010000214         2292 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.215, whole genome shotgun sequence.
ACCESSION   DASFSW010000214 DASFSW010000000
VERSION     DASFSW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.215"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(116..637)
                     /locus_tag="U1099_002098"
     CDS             complement(116..637)
                     /locus_tag="U1099_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868370.1"
                     /translation="MFISNTTKLKTGVKMRSHLGVRLSYEAKYWLESIQAVIQEKLDA
                     KINEQDIENLEHATKSYLKEVDNELGATSVTLILKASASSVLEEAFEKTKSLSLKDWH
                     KLDNEMKHSISSIPKDKDVGTLSVRFFLENSIITSLESYQKEFMTSEMVRQVRLSYVL
                     KLVIFAYYKKIMQ"
     gene            975..1757
                     /locus_tag="U1099_002099"
     CDS             975..1757
                     /locus_tag="U1099_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HEM4868371.1"
                     /translation="MKTLYHYTTVETLLLILNYKTLRFKSLLYVDDPLEPSTSDFGNL
                     GGFKYVSCWTDTPNSIPQWTMYSDNMTGVCIGISFDKETDVFLTEKFSLSESSEPIDM
                     VNALHPLKSGLLVTNNKYVPSLEQIRYTDDVSLITPRVVSSDDKFTTINLASNGIYKT
                     TDWSFQNEQRFSFQIFPLPIDLVLELMNANKGDLTGIINSFISVKPKEYFDLDLNPTI
                     FSNMTITFGKRCSAEDKLKVSKFLEDNKFHIPLFDSTVNIKP"
     gene            complement(1754..2033)
                     /locus_tag="U1099_002100"
                     /pseudo
     CDS             complement(1754..2033)
                     /locus_tag="U1099_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636196.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          715 a          353 c          369 g          855 t
ORIGIN      
        1 ctctagcctt cattagcatt ctcctttttg tgtttgtgtg taaatgtagt attggttata
       61 aacaaaggtg gttaagctac agttcctcaa tgttaccatg agaaactgta atcctctact
      121 gcattatttt tttatagtaa gcaaaaataa caagttttag aacatatgac aatctaactt
      181 gcctaaccat ttcagaagtc ataaattctt tttgataact ttccaatgat gtaataatag
      241 aattttccaa aaaaaatctt acagacaagg ttccaacatc cttatccttt ggaatagacg
      301 aaatcgaatg tttcatttca ttatctaatt tatgccaatc tttcaaactt aaagatttcg
      361 ttttttcaaa agcctcttct aaaacacttg atgcagaggc ttttaaaatc aaagttactg
      421 aagttgcccc taattcattg tcaacttctt tcaaataaga ttttgttgca tgttctaaat
      481 tttctatatc ttgttcattt attttagcgt ctaacttttc ctgaattact gcttgtattg
      541 attctaacca atacttcgct tcgtaagata gacgtactcc aaggtgtgag cgcattttta
      601 ctcctgtttt cagttttgtt gtattcgata taaacattct atcgtctata ctggcagaag
      661 tcaagcaaaa atactaaaaa aattccaaaa aatagcttca tataggtcca ttttgttgtt
      721 tgacaaagga atgaactctc aaaatgctta tttaataggc ttttgtggtt agaagctctt
      781 cgctatcaaa ctcaaaatcc agtgtccgca aggacgtgcc ggttcgaccc cggccgccgg
      841 tatagtttaa aagacaaggt ttctaagcct tgtctttttg ttttgaggtt ataaaatttt
      901 cagttgagtt actaaaaatt acttaatgtt ttgttgtata atatgttata taagcaagag
      961 gaggagccgt taatatgaag acactttatc attatacaac agttgaaaca ttgcttctga
     1021 tattaaatta taaaacattg agattcaaaa gtttacttta tgttgacgat ccattagaac
     1081 cttcaacttc agactttggt aatctaggtg gatttaagta tgtgtcttgt tggactgata
     1141 ctcctaatag tattccacaa tggacaatgt attctgataa tatgacagga gtgtgtatag
     1201 gaattagttt tgacaaagaa actgatgtct ttttaacgga aaaattttct ttatctgaat
     1261 cttctgaacc aattgatatg gtaaatgcat tacatccttt aaaatcaggc ttattagtta
     1321 caaataataa atatgtgcct tctttggaac aaataaggta tactgatgat gtttcattga
     1381 tcactcctcg agtagtttct tcagatgata aattcacaac tattaattta gcttcaaatg
     1441 gtatctataa gacaactgat tggagttttc aaaatgagca aagattttct tttcaaattt
     1501 ttccattacc aatagattta gttttggaat taatgaatgc aaataaagga gatttgactg
     1561 gaataattaa tagttttatt tctgttaaac ctaaagaata ctttgattta gatttaaatc
     1621 cgaccatatt cagtaatatg acaattacgt ttggtaaaag atgctctgct gaagataagt
     1681 tgaaagtatc aaaattttta gaggataata aattccatat tcctttattt gatagtactg
     1741 taaatataaa gccttaaatt aggtcatcta atttatcagc taaatctttt tgcttgtttg
     1801 gatacaagtg agaataggta tcgattgtag ttgttataga agcgtgtcct agcctttctt
     1861 tcacaactaa ataatcttcc cctcgattta tcaatagaga agcgtgtgag tgcttaaaat
     1921 cgtgtatgcg tatcttttta agagccgcat cacgatcaag aatttttttg tactgctttc
     1981 tattgaattt ttgtaatcgt aatgggatta ctttgaaaca cttgtaggct catgctatca
     2041 cttgtaaact gctctaatag atgtttttgt tgctgttacc aatattgcag ttcttgactt
     2101 aattttttat tgatgatatt tcttctagtt cctgctttag tttaagttgc actgatatgg
     2161 cttatgcctt tattaacgta tacagacttt gtgatatgaa ttgtattgct cgaaaggtcg
     2221 atatcttgcc gtgttagtgc gagtgcttct ctaagtctta gacctgtaaa gaataggact
     2281 gtaaagagta at
//
LOCUS       DASFSW010000215         2278 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.216, whole genome shotgun sequence.
ACCESSION   DASFSW010000215 DASFSW010000000
VERSION     DASFSW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.216"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            535..2208
                     /gene="ltrA"
                     /locus_tag="U1099_002101"
     CDS             535..2208
                     /gene="ltrA"
                     /locus_tag="U1099_002101"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM4868372.1"
                     /translation="MNDKHSTTAPAEKLSHKQLLAKQWKSIDWKRAEQEVNRLQIRIV
                     KATQAKHTNTVKRLQYLLTHSFYAKALAVRRVTTNKGKKTAGIDGELWTTPAQKMEAL
                     LSLTDKGYKASPLRRVYIDKKGKKKKRPLGIPTMYDRAMQALYALALEPVAETTADTK
                     SFGFRKGRSCQDACEYIFTALSRKASPQWILEGDIKGCFDNISHDWLLENIPMDKSIL
                     KQFLKAGFVFKGELFPTEDGTPQGGIISPILANMALDGLQQVLSDRFHTNRLGKIDLR
                     FKNSHKVNLVRYADDFIVTAATQEIALEAKELIREFLLGRGLELSEEKTLVTHINDGF
                     DLLGWNFRKYKGKLIVKPSKNSIQTVIGKFSETILKRGKAWEQEVLIMKLNQQIRGWT
                     NYHQSVCASEAFSYLDYHLYELLWRWAKRRHPKKGQWWVSTKYWHRRGNRSWVFASGD
                     KELIRVDHTAIVRHTKVRENANPYLDIDYFAQRTFNHGMKRLTGRFKLVWKKQDGRCH
                     HCGLPMELGEDREIFFKVPKSKGGVEEVDNMAYVHSYCQRLFIESRSKE"
BASE COUNT          749 a          429 c          531 g          569 t
ORIGIN      
        1 ttgtaaattg ctagtttagc tactctatcc atttggggta atcctaccac agcctgcttg
       61 actgggaacg gttatgttgg gaagcctgtg ggacaatatg gaaagtctga tagcctaaaa
      121 tcggatggac tgttaggata agaatactat ggtgaacaga cgtgaaccat cgcaagagtc
      181 gttacaggga ctaagtgaaa taaggctgat acacttacac caaaaggtac ggaggaggtc
      241 attggggctt tcataacctg atgacaaatg gacacattct tcccggctca aagatggcac
      301 ctaaggtatt cacaattatt tataacacgg aacttggtaa gccctatgta ttccttttaa
      361 tcgatagaag gtattgaact cgtaagaggg atagacgata cagagggtag aggaaaggat
      421 aaaaagcgaa tgctccaact gtaatggtag gagatagggg ttcaaacttt gccctgacct
      481 gaaagggtgc agacttatcc tatggtattt catgacaaaa atggaggtaa acctatgaat
      541 gataaacatt caacgacagc accagctgag aagttatcac acaagcaact gcttgcgaaa
      601 caatggaaaa gcattgattg gaagagagca gaacaagaag tgaataggct acaaatcagg
      661 attgtcaagg ctactcaagc caaacacaca aacacagtga aaagacttca atatttacta
      721 acccattcgt tctatgccaa ggcacttgcc gttcgtcgag taacgactaa caaaggcaag
      781 aaaacagctg gtatagacgg tgaactatgg acgacacctg ctcaaaagat ggaagctctc
      841 ctgtccctaa cggacaaagg ttacaaagcc agtccgttaa gacgggttta catcgacaaa
      901 aaggggaaaa agaagaaacg tccattaggg attccgacta tgtatgacag agccatgcag
      961 gcactctatg ctttagcact agagcctgtc gcagagacaa ctgcggatac taaatcattc
     1021 ggcttccgaa aaggaagaag ctgtcaagat gcttgtgaat atatcttcac agcactatca
     1081 cggaaagctt ctcctcagtg gattttagaa ggcgacatca aaggctgttt tgacaacatc
     1141 agtcatgact ggctcttaga gaacattccg atggataaat ctatcttaaa acaattcctt
     1201 aaagcaggtt ttgtgttcaa gggtgagtta ttcccaacgg aagacggcac gccacaaggc
     1261 ggtatcattt cgcctatcct tgccaatatg gcactcgatg gattacaaca agtcttatca
     1321 gatagatttc ataccaatcg tttaggaaaa attgaccttc gattcaagaa tagccataag
     1381 gtcaatcttg tccgttatgc ggatgatttt atcgtcacag ctgcgacaca agaaattgca
     1441 ttagaggcta aagaattgat tagagagttt ctgcttggac gaggtttaga gctgtcagag
     1501 gaaaagacat tggtaactca tatcaacgat ggtttcgacc tacttggttg gaattttcgg
     1561 aagtacaagg ggaaattgat tgtaaaacca tctaagaatt ctattcaaac tgttattggt
     1621 aaattctcag aaaccatcct caagcgagga aaagcatggg agcaagaagt cttaatcatg
     1681 aaactaaatc aacagataag aggatggaca aactaccacc aatccgtgtg tgctagtgaa
     1741 gccttctcct atctggacta tcatttatac gaattattgt ggcgatgggc aaaacgtcgt
     1801 catccaaaga aaggccagtg gtgggtttcg accaagtatt ggcatagaag aggcaatcga
     1861 agttgggtat tcgcttcagg cgataaagaa ctcattcgag ttgaccatac tgctatcgtc
     1921 agacatacaa aagtcagaga aaatgccaat ccttacttgg atatagacta ttttgctcaa
     1981 cgaaccttta atcatggtat gaaacggttg acaggtcgtt ttaaacttgt ttggaagaaa
     2041 caagacggac gctgtcatca ctgtggactc ccgatggaac ttggagaaga tagagaaatt
     2101 ttctttaaag ttccaaaatc aaagggaggt gtggaagaag ttgataatat ggcttatgtg
     2161 catagctact gtcagcgact atttattgag agccgctcga aagagtgatg aaatgcttga
     2221 gccgtatgag gggaaactct cacgtacggt tcttagagag gaaggtggta gtaatacc
//
LOCUS       DASFSW010000216         2240 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.217, whole genome shotgun sequence.
ACCESSION   DASFSW010000216 DASFSW010000000
VERSION     DASFSW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.217"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..258
                     /locus_tag="U1099_002102"
     CDS             <1..258
                     /locus_tag="U1099_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM4868373.1"
                     /translation="KDKWYEVTGIILDGKLYRLRIRRLTPRECFRLQGFPDWAFERAE
                     SVSSKSQLYKQAGNSVTVTVIEAIAREFRKIEEEEEHEIIT"
     gene            242..670
                     /locus_tag="U1099_002103"
     CDS             242..670
                     /locus_tag="U1099_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868374.1"
                     /translation="MKLLHSKSIRNCTELEEAIHQAEVERFSEMIASLPNYDCDIDVT
                     FEDDYHKEMNYPLAYESNLHRIFEFIETQDIKNGVDTYLTDENDLAFRAYGQHYMANG
                     QDGLLTTLITVKSFGEGRSPIDMSKVFTPPTQALEKELSV"
     gene            680..1063
                     /locus_tag="U1099_002104"
     CDS             680..1063
                     /locus_tag="U1099_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868375.1"
                     /translation="MLEIYLSRNTSRNQKLLSFCDSHGISYSSKEVSNLSREDLLGLF
                     TKSSDCFTLLSPSLQRFKGHREMKLSELVTVVLRKPDQNLRLPIVVCEDKVYPDMSLE
                     EARTFLPRSQKVVSFREHLFKDLVT"
     gene            1073..1306
                     /locus_tag="U1099_002105"
     CDS             1073..1306
                     /locus_tag="U1099_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM4868376.1"
                     /translation="MNDRFWENLEIIVMEKGLSWADLARQMFKGQYVYPSEFKRLYQT
                     FRHYKSHRLMPQGKWVEKIVVVLEIDYEDLFRR"
     gene            1309..1890
                     /locus_tag="U1099_002106"
     CDS             1309..1890
                     /locus_tag="U1099_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM4868377.1"
                     /translation="MKRLFVLYGAIHVNVLLVSLYLVGWLNGAWLPVLQVTFLALLLW
                     GWKRFKIPKRNLSFKERGLWVCGSLGVMVSIALIMSALFSVSEPNQETLVTVQDQIPV
                     LSFILFLLNASVVEEVFYREVLWGVLSQPVIQVLLTSSLFALAHHSSSLFTWVLYGSL
                     GFTLGVVRGQTDCLSSTLVHLSWNGIVFFLSLL"
     gene            1967..>2240
                     /locus_tag="U1099_002107"
     CDS             1967..>2240
                     /locus_tag="U1099_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868378.1"
                     /translation="MSSEQQERQAIQYAERSAMFTVKVLLKLLEWSARHALAQDSAYK
                     VGVQKLEELLQSPYAIESLNISKDILDKPVDVEKFKELVESENLPIA"
BASE COUNT          661 a          387 c          524 g          668 t
ORIGIN      
        1 aaggacaagt ggtatgaagt gactggaata atcctagatg gaaaactgta tcgtttgaga
       61 atcagacgcc taaccccaag agagtgtttt aggttacaag gatttcctga ctgggctttt
      121 gagagggcag aaagtgtttc aagtaaaagt cagttgtata aacaagcagg caatagcgtg
      181 accgtcactg ttatcgaggc cattgctaga gaatttagaa agattgaaga ggaagaagaa
      241 catgaaatta ttacatagta aaagtattag gaactgcacg gagctagaag aagccataca
      301 tcaagcagaa gtggagagat tttcggaaat gattgcttct ttacccaatt atgattgtga
      361 cattgatgtc acttttgaag atgactacca caaagaaatg aattatccgt tagcttatga
      421 gtccaactta caccgtattt ttgagtttat tgaaacccaa gacatcaaaa atggtgttga
      481 tacttatcta acggatgaga acgatctcgc ctttagggca tatggtcaac attatatggc
      541 aaatggacaa gatggtttat tgactacact gattacagta aagagttttg gtgaaggcag
      601 gtcgcctatt gatatgagta aggtgtttac cccaccaact caagcattag agaaggagct
      661 atctgtgtag gaggatacga tgttagaaat ctatttaagt cgaaatacca gtcggaatca
      721 aaaactcctt tctttttgtg actcgcatgg tatttcctac tccagtaaag aagtaagtaa
      781 cctgtcacgt gaagacttat tgggcttgtt taccaaaagc agtgattgtt ttacgctctt
      841 atccccctcg ctacaacgat ttaaaggtca tagagagatg aaattgagtg agttagtgac
      901 ggtggttcta cggaaacctg accaaaatct tcgtctccca attgtggttt gtgaggacaa
      961 agtgtatccc gatatgagtt tagaagaagc aaggaccttt cttccgagaa gtcaaaaagt
     1021 ggtttccttt cgtgaacatc tttttaagga tctggtgaca taaggaggag ttatgaacga
     1081 tcgtttttgg gaaaatttgg aaattatcgt tatggaaaaa ggactgtcat gggctgacct
     1141 tgctcgacag atgtttaagg gacaatacgt ttatcctagt gagtttaagc gcctctacca
     1201 aacctttcgt cactacaaat cccatcgtct catgccacaa ggtaaatggg tggagaaaat
     1261 agtggttgtt ttagagattg actatgaaga cttgtttagg agatagcgat gaaacgacta
     1321 tttgtgttgt acggagccat tcatgtcaat gtactcctag tgagtttgta cctagttggt
     1381 tggctaaatg gggcatggtt acctgtttta caagtgacat ttttagccct gctattgtgg
     1441 ggttggaaaa ggtttaagat accgaaaaga aacctatcct ttaaggagag agggctttgg
     1501 gtgtgtggta gtctaggtgt catggtaagt attgctctta ttatgagtgc tctattttca
     1561 gtaagcgaac caaaccaaga aacgttagtg actgttcaag accagattcc tgttctgtcc
     1621 tttatccttt ttctccttaa tgccagtgtt gtggaagaag tcttttatag agaggtgttg
     1681 tggggagtct tgtctcaacc agtgattcaa gtgttgttaa cgagttccct ctttgcctta
     1741 gctcatcact catccagtct attcacttgg gtgctttatg ggagtttagg tttcacactt
     1801 ggtgtggtta gagggcaaac agactgcttg agctcaactc tcgttcacct gtcttggaat
     1861 ggaatcgtct ttttcctatc tttattgtga taccaaaatc aagggtgctt tcgtatcatg
     1921 aaagcaccct attgttttga tggggaatca tataaaggag gaaatgatgt catccgaaca
     1981 acaggaacgc caagcgattc aatatgcaga acggagtgct atgtttacgg tgaaggtcct
     2041 tctcaaactc ttagagtggt cagccagaca cgccctagca caagactcgg cttataaagt
     2101 tggggttcaa aagttagaag aactccttca aagtccatat gccattgagt cgctgaatat
     2161 ctcaaaagac attttggata aaccagttga tgtggagaaa ttcaaggaat tggtggaatc
     2221 agaaaacctt cctattgcca
//
LOCUS       DASFSW010000217         2221 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.218, whole genome shotgun sequence.
ACCESSION   DASFSW010000217 DASFSW010000000
VERSION     DASFSW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.218"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            60..236
                     /locus_tag="U1099_002108"
     CDS             60..236
                     /locus_tag="U1099_002108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868379.1"
                     /translation="MTNQEYHSKAGKLLQTIREFYLRPGKGTAKECIKPSKGFSSKNL
                     EPYIFKEYFAKYLR"
     gene            562..921
                     /locus_tag="U1099_002109"
     CDS             562..921
                     /locus_tag="U1099_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868380.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDYKQLAAKEWFSLWQSQKFEQISRDVHEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VSQVQPLSREFREKYMG"
     gene            931..1296
                     /locus_tag="U1099_002110"
     CDS             931..1296
                     /locus_tag="U1099_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEM4868381.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSHCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQEQLQELSKGVSELITG
                     VEKEFQVEVKRLKEFHGSH"
     gene            1283..>2221
                     /locus_tag="U1099_002111"
     CDS             1283..>2221
                     /locus_tag="U1099_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM4868382.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYKEMVEMYNVNFTNNDKLYESRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETMMELTGGLFKFIVATHTDKDHTHNHILVNAIDSNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRYSYRDYQKYRQSSHKFELKQRLYFLMQQSKSFDDFLEKAVQLH
                     VHIDFSQKHSRFIMTDRAMTKPIRGRQLSKRDLYDEDFFRTHFAKQEIESRLEFLLNC
                     VNSLEELLTKAKELNLTIDLKQKNVVFTLEENGKQIS"
BASE COUNT          773 a          358 c          433 g          657 t
ORIGIN      
        1 cagataattt atattatgga aatatatttg ccttaacggt cgataatcgg ttagaagaga
       61 tgacaaatca agagtatcat tccaaagcag gtaaattact tcaaactatt agagaatttt
      121 atcttcgacc agggaaaggg actgcaaaag aatgtattaa accatctaaa ggtttttctt
      181 cgaagaattt agaaccgtat atttttaaag agtattttgc taaatattta agatagtttc
      241 cgagagctac ccagctctct tttttcttac tctcattttt catactgtga taacttttcc
      301 aaatcatcct tgttatccta atatcattcc taagaaaatc aaagtttgat tttctattgg
      361 gggtttgggg gcgaagccac caagttatct tatcgttggc tgtcaaaact ggaaggtttt
      421 ggtcggctgg cgataagatt tttgggatat tgtggacaca atatctgagc tcgcaaagac
      481 cgaacaagga ctttaagaaa tttcttgtat gacacacggg gtcggggttt acccgagaaa
      541 ggatgaaagt gagggaaaga gatgtctgaa cagtaccgtg acattagaaa agaggtaaat
      601 ctaaccgcag atgagctaaa acagattgaa aaaatgatgg aggttgataa ttatcgtcac
      661 ttttcaccat tcgtcaggga taaaattttg atgactgatt ataaacagtt agctgccaaa
      721 gaatggtttt ccctttggca gtctcaaaag tttgaacaga taagtcgaga tgtgcatgaa
      781 gttctagttg tcgcaagaga aaatcaccaa gtgactcaag aacacgtgtc aatcttattg
      841 acctgcgttc aagaattgat tgcggaagtc agtcaagtac aaccactcag tcgtgagttt
      901 cgtgaaaaat atatgggcta ggagggtgta atggtttatc gctatcgtac caatctcaaa
      961 aaagtatttt taacagattc agagttacat caattgaatg aacgaattgc taagagtcat
     1021 tgtcaaaatt tctcagtcta tgctagaaaa gttctgctta atcccaatat gtcctttgtc
     1081 acgattaaca ctgataccta tgagcagtta gtgtttgaat tgagacggat tggaaataac
     1141 attaatcaaa ttgcgcgtgc gatcaatcaa agccatctga tttcccagga acagttacaa
     1201 gaattaagta agggagttag tgaattaatt actggagtag agaaagaatt tcaagtcgaa
     1261 gtgaaaagat taaaggagtt tcatggtagt cactaaacac tttgcgacac atggaaaaaa
     1321 atatcgtagg cgtttaatta agtatattct caatcctgat aaaacggaca atttgaaatt
     1381 ggtatctgat tttggcatga gcaattattt agacttccct agctataaag aaatggtaga
     1441 aatgtacaat gtcaacttta ccaataacga caagttgtac gaatctagaa atgaccgaca
     1501 agaaaaacac caacaaaata ttcatgccca tcacctcatt caatcatttt ctcctgaaga
     1561 taatctgaca cctgaagaaa ttaaccgcat tggttacgag actatgatgg aattgacagg
     1621 aggtctcttt aagtttatcg tagcgactca tacagacaaa gatcataccc acaaccatat
     1681 cttagtcaat gccatagaca gtaattcaga taaaaaattg atatggaatt atgccttaga
     1741 acgaaattta cgtatgattt cagaccgtat ttctaaaatg gcgggggcaa aaattatcga
     1801 gaagcgttac tcctaccgtg actatcaaaa atataggcag tctagtcata aatttgaatt
     1861 gaaacaacgt ctgtattttt tgatgcaaca gtcaaagtcc tttgatgatt ttttagaaaa
     1921 agcggtgcag ttacatgttc atattgattt tagtcagaag catagtcgat tcataatgac
     1981 ggatagagca atgacaaaac caattcgagg acgccaactc agcaaacgag atttatatga
     2041 tgaagacttt ttccgtacac attttgctaa gcaagagatt gaaagtcgtt tagaattttt
     2101 gttgaactgt gtcaattctt tagaagagtt actaacaaaa gcaaaagaat tgaatctaac
     2161 cattgacttg aaacaaaaaa atgttgtatt tactcttgaa gaaaatggca agcagattag
     2221 t
//
LOCUS       DASFSW010000218         2197 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.219, whole genome shotgun sequence.
ACCESSION   DASFSW010000218 DASFSW010000000
VERSION     DASFSW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.219"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            33..677
                     /locus_tag="U1099_002112"
     CDS             33..677
                     /locus_tag="U1099_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEM4868383.1"
                     /translation="MTELLDIYRTLKSKTWVDLTHQINEKSPNFPALPALEKKALFTH
                     KDGFFVEQFTVVGQYGTHLDPPIHFVEGARYLDEIDLKDLLLPLYVIDKSAAVIENND
                     YEITKQDILDFEAEYGPIAPKSFVAFRSDLSKRWPSQDAIRNLDEDGVQRTPGWSHEA
                     LEYLIEERQVKAVGHETLDTDLGVSAAKHGGSLSEEYYLLSKDIYQLEVLANLD"
     gene            complement(821..2170)
                     /gene="gorA"
                     /locus_tag="U1099_002113"
     CDS             complement(821..2170)
                     /gene="gorA"
                     /locus_tag="U1099_002113"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEM4868384.1"
                     /translation="MKVFDIIAIGGGSGGIATMNRAAMYGAKAAVIEGSYLGGTCVNL
                     GCVPKKIMWYGSQVAEAIQHYGPEYGFTSQEVTFDFATLRKNREAYIERSRASYGNTF
                     NNNGVEVIQGFARFVDNQTVEVNGELIRAKHIVIATGAKPVVPAIPGSELGIVSDDVF
                     AWEELPSSVAVIGAGYIAVEMAGLLHGLGVQTDLFVRGNRPLRSFDTYIVDALMEEMQ
                     RTNLPLHTGKTPVSLEKTDDGQIQINFADGSNHIAQKVLWAIGRKPNIDKLNLKATSV
                     QLTSSGHISVNEYQETAVPGIYALGDVTGEKELTPVAIKAGRLLAERLFNNKPTAKMD
                     YTTIPTVVFSHPAIGTVGLTEDEAITQYGQDNVKVYTSAFTSMYTALENNRQMAKFKL
                     VTVGENEKVVGLHGIGYGVDEMIQGFSVAIKMGATKEEFDAVVAIHPTGSEEFVTMR"
BASE COUNT          636 a          464 c          462 g          635 t
ORIGIN      
        1 tagaataata aaaaaagaaa gtgagaattt ctatgacaga attacttgat atttaccgta
       61 ctcttaaatc taaaacttgg gtagacttga ctcaccaaat caatgaaaag agcccgaatt
      121 tccctgcgct accagctctc gaaaagaaag ccctatttac tcataaggat ggcttctttg
      181 tcgaacaatt tacggttgtg gggcagtacg gaacccatct tgatcctccg attcattttg
      241 tagaaggggc tcgttatctt gatgagattg atttgaaaga tttactcctg ccactctacg
      301 ttattgataa atcagctgct gttatagaaa ataatgacta tgagataact aagcaggata
      361 ttttagactt tgaggctgaa tatggtccga tagcgccaaa atcttttgtt gcctttcgtt
      421 cggatttgtc aaagcgctgg cccagtcaag atgccattcg taatctagac gaagatggag
      481 ttcaacgtac accaggatgg agtcatgaag ctctagaata tttgattgaa gaacgtcagg
      541 tgaaagcagt tggtcatgaa acattggata ccgatttagg agtatctgca gccaaacacg
      601 gaggaagttt atcagaagaa tactatctcc tatcaaaaga tatttatcag ttggaagtat
      661 tggcaaactt ggattaggta ccgccaacag gtgcattgat ttcaatcgct tttcctcatt
      721 gggagaaggc ttcgggttca ccagtgcgag caattgccat attaccataa aaaaaagtcc
      781 agacagtgct gaccttcatg tctggacttt ttcaattgag ttaacgcata gtaacaaatt
      841 cttcagaacc tgtcggatgg atggcgacaa cggcatcaaa ttcttctttt gtagctccca
      901 ttttgatagc aactgagaat ccttgaatca tttcatcgac accatagcca atgccgtgta
      961 gtccaacgac tttttcattt tctcccacag ttactaattt aaacttagcc atttggcgat
     1021 tgttttctag ggcagtgtac atagaagtaa aggctgaagt atagactttt acattgtcct
     1081 gaccgtattg ggtaatcgct tcgtcctcag ttagaccaac ggttccgata gcggggtgag
     1141 aaaagacgac agttgggatg gttgtgtaat ccatttttgc ggttggttta ttgttaaata
     1201 aacgttctgc caaaaggcga cctgccttga ttgcaacggg tgttaattct ttttcaccag
     1261 ttacatcacc gagggcatag atgcctggta cagctgtttc ttggtattca ttgacagaaa
     1321 tatgtcctga agaagtaagt tgaacactag ttgctttcag gttgagtttg tcaatatttg
     1381 gtttacgtcc aattgcccaa aggaccttct gagcaatatg gttactaccg tcagcaaagt
     1441 taatctggat ttgtccatca tcagtttttt caagactgac aggagttttc ccggtatgga
     1501 gtggaaggtt ggtacgttgc atttcttcca tcaaggcatc aacaatgtag gtatcaaagc
     1561 tacgaagagg gcgattacca cgaacaaata ggtcagtttg gacaccgagt ccatgtagga
     1621 gaccggccat ttccacagcg atataaccag caccaatcac agccacagaa gagggtaatt
     1681 cttcccatgc aaagacatcg tcagatacaa tccctagctc actaccagga atagcaggga
     1741 ctaccggttt ggcgccagtt gcgatgacaa tgtgttttgc acgaatcagt tcaccattga
     1801 cttcaaccgt ttgattatcc acaaaacgtg cgaaaccctg gataacttct actccgttat
     1861 tgttaaaagt attaccgtag gatgcgcgtg aacgctcaat ataggcttcg cgatttttac
     1921 gcaaggtggc aaaatcaaag gtcacttctt ggctagtaaa tccatattca ggtccataat
     1981 gctgaatggc ttctgctacc tgagagccgt accacataat ttttttgggt acacagccaa
     2041 ggttaacaca agtccctcct agatagcttc cctcaataac tgctgccttg gcaccgtaca
     2101 tagcagctcg gttcatggta gcgatgcctc cgctcccacc accgatagcg ataatatcaa
     2161 acactttcat aaatttcctc caataaaatc attaact
//
LOCUS       DASFSW010000219         2188 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.220, whole genome shotgun sequence.
ACCESSION   DASFSW010000219 DASFSW010000000
VERSION     DASFSW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.220"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1773
                     /locus_tag="U1099_002114"
     CDS             <1..1773
                     /locus_tag="U1099_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEM4868385.1"
                     /translation="ARDLLEATLELLEKSRHTELDPAAVAKELTGLIADDKVQQEGTK
                     IFDNSLYFAEHGIHKNLTRLMGKNGFKPFPRTDVEAAIAELETMSSLTYDDIQKEAIV
                     QAITNPLFILTGGPGTGKTTVINGIIAVYAILHKIDLTRNREECPVLLAAPTGRAARR
                     MNELTGLPSATIHRHLGLVEGQEESYRDDYLDADFIIVDEFSMVDTWLANQLFQNISS
                     QTQVLIVGDAEQLPSVSPGQVLADLLKIDKLPSITLERIYRQSDDSTIVTLASQIRQG
                     ALPSDFREKKADRSYFEAQNEQIPALIERIVGAAIKSGIPANEVQILAPMYRGAAGID
                     QLNTMTQALLNPLEEGELEFLHNEQAFRQGDRVIHLVNDAEANVFNGDLGYITDLLPA
                     KYTDSKQDEITINFDGSEVTYPRNEWYKITLAYAMSIHKSQGSEFQVVILPITRTSHR
                     MLQRNLVYTAITRSKSKLILLGEISAFDYAVKNAGTVRKTYLVPRFQGEMAEQESKES
                     LIKKDESKTATTTQPQPPTQPDRKEQVKVVKENNQQLSLLDQDQPKKTSPQPTEYILT
                     VDNLLTIDPMIGIEQADIEEFFKA"
     gene            complement(1812..>2188)
                     /locus_tag="U1099_002115"
     CDS             complement(1812..>2188)
                     /locus_tag="U1099_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEM4868386.1"
                     /translation="KETSRQAVQALQTMELEVVMLTGDNEKTAQAIAKEVGIEQVVSQ
                     VLPDDKANQVKFLQEQGKTVAMVGDYDHAFGKSYGVLMREWNLLARAVFVLNADNEIV
                     YVEYLDNVNEHPDYKAALEAVK"
BASE COUNT          610 a          620 c          465 g          493 t
ORIGIN      
        1 gccagagact tgctggaagc aactcttgaa ctgctggaaa aatcccgcca tacagagctg
       61 gaccccgctg ctgttgccaa agaattgacg ggactgattg cagacgacaa ggtacagcaa
      121 gaaggcacaa agatttttga caacagcctc tactttgccg aacatggtat ccataaaaac
      181 ttgacccgcc tgatgggaaa aaatggtttc aaacccttcc cacgcacaga cgttgaggct
      241 gctatagcag agctggaaac tatgtcttcc ctgacctacg atgacattca aaaagaagcc
      301 atcgtccaag ccattaccaa tccgcttttc attctgacag gcggaccagg gactggaaag
      361 acaaccgtta tcaacggtat tatcgcggtc tatgccatct tgcataagat tgacctgact
      421 cgcaaccgag aagaatgccc agtcctccta gccgctccaa ctggacgagc agccagacgg
      481 atgaatgaat tgacaggttt gccttccgcc accatccacc gccatctcgg tctggtagaa
      541 ggacaggaag aatcctaccg tgatgattat ttggatgccg actttatcat cgtggatgag
      601 ttttctatgg tagatacttg gttggcaaac cagctcttcc agaatatctc ctcccagacc
      661 caagttctga ttgtcggcga tgccgagcaa ttgccttctg tcagccccgg tcaagtactt
      721 gctgacctct tgaaaattga caagctgccc agcattactc tagaacgtat ctatcgtcaa
      781 tccgatgatt cgaccattgt taccctagcc agccagattc gacagggtgc tctgcctagc
      841 gattttcgtg agaaaaaggc tgaccgttcc tattttgaag cccagaatga acaaattcca
      901 gccctgattg agcgcattgt cggcgcagcc atcaagtcag gtattcctgc aaacgaggta
      961 cagatcctcg ctcccatgta ccgtggggcc gcaggcatcg accaactcaa cactatgacc
     1021 caggccctgc tcaatccact ggaagaggga gagttagaat tcctgcataa cgagcaagcc
     1081 ttccgccaag gcgaccgggt cattcattta gtcaacgatg ccgaggccaa tgtcttcaat
     1141 ggtgatttag gctacatcac cgacctcctt cctgccaagt acaccgactc caagcaggac
     1201 gaaattacca tcaacttcga cggtagcgag gtcacctatc cccgaaacga atggtacaag
     1261 attaccctgg cctatgccat gtccatccac aagtcccaag gcagcgagtt tcaggtagtc
     1321 atcctcccca tcacccgcac cagccaccgt atgctccagc gcaatctggt ctacaccgcc
     1381 atcacccgct ccaagagcaa gctcatcctc ctgggcgaaa tctccgcctt cgactacgcc
     1441 gtcaaaaacg ccggcaccgt ccgaaaaacc tatctggtcc cacgtttcca aggggaaatg
     1501 gcagagcagg aaagcaagga aagcctgatt aaaaaagacg aaagcaaaac agctacaacc
     1561 acgcaacccc agccccctac tcagccagac agaaaagaac aggttaaagt agtcaaagaa
     1621 aacaaccaac aactctccct tcttgatcaa gaccagccaa aaaaaacaag tccccaaccc
     1681 acagaataca ttttgactgt agacaaccta ctaaccatcg accccatgat tggcatagaa
     1741 caggcagata ttgaagaatt ttttaaagca taaaaaccag caagtcagta gatgaacacc
     1801 gttatattct tttatttgac agcctctaaa gctgctttat aatctgggtg ttcgttgaca
     1861 ttgtctaggt attctacgta gacgatctca ttgtcggcat taaggacaaa gaccgcacgc
     1921 gccagcaaat tccactcccg catgagcaca ccataggatt ttccaaaggc atggtcatag
     1981 tcccctacca tggcgactgt cttgccctgt tcttgtaaga acttgacttg gttggccttg
     2041 tcatctggca ggacctgact gaccacctgc tcaatgccga cttccttggc aatagcctgg
     2101 gcagtttttt cattatctcc tgtcagcatg acaacctcta gttccatggt ttgcagagcc
     2161 tggacagcct gacggctggt ctctttta
//
LOCUS       DASFSW010000220         2183 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.221, whole genome shotgun sequence.
ACCESSION   DASFSW010000220 DASFSW010000000
VERSION     DASFSW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.221"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            403..762
                     /locus_tag="U1099_002116"
     CDS             403..762
                     /locus_tag="U1099_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868387.1"
                     /translation="MGQEIKLIRKQFRITRQEEIQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKLEQISRDVHEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMR"
     gene            772..1137
                     /locus_tag="U1099_002117"
     CDS             772..1137
                     /locus_tag="U1099_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEM4868388.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSHCQNFSVYARKVLLN
                     PNMSFVTINTETYDQLVFELRRIGNNINQIARAINQSHLISQEQLQELSKGVSELITG
                     IEKEFQVELKRLKEFHGSH"
     gene            1124..>2183
                     /locus_tag="U1099_002118"
     CDS             1124..>2183
                     /locus_tag="U1099_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM4868389.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGISNYLD
                     FPSYEEMVDMYNVNFTNNDKLYESRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETMMELTGGRFRFIVATHTDKNHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRYSYRDYQKYRQSSHKFELKQRLYFLMQQSKSFDNFLEKAVQLH
                     VHIDFSQKHSRFMLIDRAMTKPIRGRQLSKRDLYDEEFFRTHFAKQEIESRLEFLLNC
                     VNSLEELLLKAKELNLTIDLKHKNVTFILEEDNQKISLGHKKISDKKLYDVNFFQDYF
                     KNKEVGDSEGLDNLREQYH"
BASE COUNT          771 a          346 c          420 g          646 t
ORIGIN      
        1 ttcatctatc aaaactattg ggatatttta agattaccta tttggtactt taagtgtaaa
       61 tacaaaaacc actgagagca ttcagctctc ttttttctta ccttctacag taatgtgata
      121 acttttccaa ataatccttg ttattcttat atcattccta agaaaatcaa gtcttgattt
      181 ttcactgggg gtttgggggc gaagccacca cattttctaa tcgtcggctg accaacctgt
      241 aaggtttggg gtcagctgac gattagattt tgggttattg tggacacaat aactgagctc
      301 gcaaagaccg aacaagaaat gagtaaggtc tttaggaaat gtactgacaa tgtgaggcag
      361 cctgacactg tcagacaagt ataatgttga aaggatagta ggatgggaca agaaattaag
      421 ttaatccgta agcaatttag aatcacgaga caagaagaaa tacagataaa agaaatgatg
      481 agggaacaaa aagtggatag tttctcagaa tttcttcgtc aaaatttgtt gaaaaagaat
      541 tatcaggata gaatttttga aagttggttt tccctttggc agtctcaaaa gttggaacaa
      601 attagtcgag atgtgcatga agttctagtt gtcgcaagag aaaatcacca agtgactcaa
      661 gaacacgttt caatcttgtt gacctgcgtt caagaattga ttgcggaagt aaatcaagta
      721 cagccactca gtcgtgagtt tcgtgaaaaa tacatgagat aggaggctac tatggtctat
      781 cgttatcgta ccaatctcaa aaaagtattt ctaacagatt ccgaattaca tcaattgaat
      841 gaacgaattg ctaagagtca ctgtcaaaat ttctcggtct atgccagaaa agtgttgctt
      901 aatcccaata tgtcttttgt cacaattaac actgaaactt atgaccagtt agtttttgaa
      961 ttgagacgga ttggaaataa cattaatcaa attgcgcgtg cgatcaatca aagtcatctg
     1021 atttcccagg aacagttaca agaattaagt aagggtgtta gtgaattaat tactggaata
     1081 gagaaagaat ttcaagtgga attgaaaaga ttgaaggagt ttcatggtag tcactaaaca
     1141 ttttgcgacg cacggaaaaa aatatcgcag gcgtttgatt aagtatatcc tcaatcccga
     1201 taaaacggac aatttgaaat tggtatctga ttttggcata agcaattact tagactttcc
     1261 cagctatgaa gaaatggttg acatgtacaa tgtcaatttt accaataatg acaagttgta
     1321 cgaatctaga aatgaccgac aagaaaaaca tcaacaaaat atccatgccc atcacctcat
     1381 ccaatcattt tctcccgagg ataatctgac acctgaagaa attaaccgca ttggttatga
     1441 gaccatgatg gaattaacag gaggccgttt tcgttttatc gtggcaactc atacagacaa
     1501 aaatcatgtt cataatcaca tcctaatcaa cgccattgat cgtaattcag ataaaaagtt
     1561 gatatggaat tatgccttag aacgaaacct tcgtatgatt tcagaccgca tttctaaaat
     1621 ggcaggggca aaaattatcg agaagcgtta ctcgtatcgt gactatcaaa aatataggca
     1681 gtctagtcat aaatttgaat taaagcaacg tctttatttt ttgatgcagc agtcaaagtc
     1741 ctttgataat tttttagaaa aagcggtgca gttacatgtt catattgatt ttagtcagaa
     1801 gcatagtcga ttcatgctta tagatagagc tatgacaaag ccaattcgag gacgccaact
     1861 cagtaaacga gatttatatg atgaagaatt ttttcgtaca cattttgcca agcaagagat
     1921 tgaaagtcga ttagaatttc tgttgaactg tgttaattca ttagaggagc tactattaaa
     1981 agcaaaagaa ttgaatctaa ccattgactt aaagcacaaa aacgtaactt ttatcctgga
     2041 agaagataat caaaagataa gtttgggtca taaaaaaata agtgataaga aattatatga
     2101 tgttaatttt tttcaagatt attttaaaaa taaggaagtc ggggattcag aaggattaga
     2161 taatttacgg gaacagtacc atg
//
LOCUS       DASFSW010000221         2173 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.222, whole genome shotgun sequence.
ACCESSION   DASFSW010000221 DASFSW010000000
VERSION     DASFSW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.222"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(42..2060)
                     /gene="recG"
                     /locus_tag="U1099_002119"
     CDS             complement(42..2060)
                     /gene="recG"
                     /locus_tag="U1099_002119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEM4868390.1"
                     /translation="MKRLCDELSVLPGIGPKSAEKFLKINIQNINDLLTYYPFRYEDF
                     ESKSIYDLQDGEKAVVVGEVVSPANVQYYGYKRNRLRFSMKQGEVVLAVSFFNQPYLA
                     DKIVLGQDIAVWGKWDKAKASLTGMKILAQVSDELQPVYHVAQGISQVNLVKAIKTAI
                     DQGYLHLLEENLPSVLRERYRLMNRREAVFAMHFPTNLEEYRQALRRMKFEELFYFQL
                     QLQMLKANNRDISNGLKIAYDADRLAMQIRQLPFVLTDAQSGALAEILSDMKSYGHMN
                     RLLQGDVGSGKTVVAGLAMFAAVTAGMQAAIMVPTEILAEQHFESLRQLFPELSIALL
                     TGGMKAAERRTALEAISSGQVDIIVGTHALIQESVTYHKLGLVVTDEQHRFGVKQRRL
                     FREKGDNPDVLMMTATPIPRTLAITAFGDMDVSIINQLPAGRKPIITRWVKHQQLPTV
                     LDWLERELEVGAQVYFISPLIEESEALDLKNAVDLQSDLQAHFGEQVTVDLLHGKMKN
                     DEKDAIMQAFKERKTNILVSTTVIEVGVNVPNATVMVIMDADRFGLSQLHQLRGRVGR
                     GHKQSYAVLVANPKTESGKERMKIMTETTDGFILAEADLKMRGSGEIFGTRQSGLPEF
                     QVANIIEDYPILEEARRVASQIVSVENWQEDPNWSILLANLKDREELD"
BASE COUNT          693 a          560 c          325 g          595 t
ORIGIN      
        1 gtcaaaagac caacacaggt gtgttggcct tttttatatg actagtccaa ttcctctcga
       61 tctttcaaat tagcaagcaa aatcgaccaa ttagggtcct cctgccaatt ttccacgcta
      121 acaatctgac tagcaactcg tcttgcttcc tctaaaattg gatagtcctc gataatattt
      181 gcaacctgaa attctggcaa acctgattga cgagtcccaa atatttctcc agacccacgc
      241 atttttaagt ctgcttcagc caaaataaaa ccatctgtcg tctctgtcat aattttcatg
      301 cgttccttac cagactctgt tttaggatta gcaactaaca ctgcataaga ctgcttatga
      361 ccacgtccta cacgccctct caactgatgt aattggctta aaccaaaacg atctgcatcc
      421 ataataacca tcaccgttgc attgggcaca ttgactccga cttcaataac agtggtagaa
      481 accaaaatat tcgtttttcg ctccttaaaa gcctgcatga tggcatcttt ctcgtcattc
      541 ttcatcttac cgtgtaacaa atccactgta acttgctctc caaaatgcgc ctgtaaatca
      601 gattgcagat ctacagcatt ctttaaatct agagcctcag attcttcaat tagcggagag
      661 ataaaataaa cctgagcacc cacttctaac tcccgctcca accaatctaa gaccgtgggc
      721 agctgttgat gtttgaccca gcgagtgata attggcttcc gaccagcagg taactgatta
      781 ataatcgata catccatatc tccaaaagct gtaatggcta atgtccgagg aataggtgta
      841 gcagtcatca taagcacatc cggattatca cccttctcac gaaacagacg gcgctgttta
      901 acaccaaaac gatgttgctc atccgtcaca accaaaccaa gcttatggta cgtcacactt
      961 tcctgtataa gagcatgagt tcctacaatg atgtctacct gaccactaga aatcgcctct
     1021 aaagcagtac gacgctccgc agccttcatt ccacctgtca aaagagcaat agaaagttct
     1081 ggaaaaagct ggcgcaaact ctcaaagtgt tgctcagcca aaatttctgt cggcaccata
     1141 attgcagcct gcatccccgc agtcacagca gcaaacatag ccaatccagc aactactgtc
     1201 ttccctgaac caacatcccc ttgtaacaaa cgattcatgt gcccatagga cttcatatct
     1261 gatagtattt cagccaaagc cccagactga gcatctgtta agacaaacgg caattgtctt
     1321 atttgcatag ccaatctatc agcatcatag gcaatcttca aaccattcga aatatcacga
     1381 ttgttggctt tcagcatttg caactgtaat tgaaaataaa ataattcctc aaacttcata
     1441 cgtcgcaaag cttgtctata ctcttctaaa tttgtaggaa aatgcatcgc aaaaaccgcc
     1501 tctcggcgat tcattaaccg atagcgctct cgtaaaactg atggcaagtt ctcctctaat
     1561 aaatgcaaat atccctgatc aatagctgtt ttaatagcct taaccaaatt tacttgcgaa
     1621 atcccttgcg ccacatgata gacaggttgg agctcatctg atacttgagc caaaattttc
     1681 attcctgtca aacttgcctt agccttatcc cactttcccc aaactgcaat atcctgcccc
     1741 aaaacaatct tatctgctaa ataaggctga ttaaaaaacg aaactgctaa gacaacctca
     1801 ccctgtttca ttgaaaaacg gagtctattt cttttgtaac cataatactg tacattagct
     1861 ggagatacca cctccccaac taccactgcc ttctccccat cctgcaaatc ataaatagac
     1921 ttactctcaa aatcttcata tctaaatgga tagtaagtca gcaaatcatt aatattctga
     1981 atattaattt ttaagaattt ttcagccgat tttggaccaa tccctggcaa aaccgataat
     2041 tcatcacata atcttttcat gtctctatta tatcaacaaa caatgaatag acaaagtaac
     2101 aagaaaagaa aaaagagcca aattaaagcc catacacctc aaaaaacaaa aaaaagagac
     2161 ctaagcagcc tcc
//
LOCUS       DASFSW010000222         2172 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.223, whole genome shotgun sequence.
ACCESSION   DASFSW010000222 DASFSW010000000
VERSION     DASFSW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.223"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>2172
                     /locus_tag="U1099_002120"
     CDS             <1..>2172
                     /locus_tag="U1099_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucan-binding protein"
                     /protein_id="HEM4868391.1"
                     /translation="KVVPKTFTPKTFTPEPPVSFKEKPLEKVTQPSLTLTKVTLPKDP
                     TPEPLPKAPQVPTVHYHDYRLTTTPEIVKEVINTDQVNLHDKTVAKDSTVIYPLTVDA
                     FSPNRAKTTSLTFEDYLPVGYAFDKEKTQAENGNYTLSFDAAKNFVTLTAKEALLQEI
                     NKDLTKTYQLVAPKLYGNLQNDGATYSNSYKLFINKGTPNAYTVTSNVVTVRTPGDGT
                     TTSRIEPQKRNENEDGVVINDTVVALGTTNHYRLTWDLDQYKGDRSSKETIARGFFFV
                     DDYPEEVLNLVDKGTSITTPEGKAVLGITVKAYASLSEAPKDIQDKLAHAKISPKGAF
                     QVFMPNDNQAFYDQYVKTGTSLNLLTKMTVKDSLYGQTKTYRNKAYQVDFGNGYETDE
                     VTNTLVSPAPKKQNLNKDKVDINGKPMVVGSQNHYTLSWDLDQYRGIKADKAQIAKGF
                     YFVDDYPEEALLPDEASIQLVTTDGKAVSGVTVKTYTNLSEAPKNLQAALSKRKIAPK
                     GAFQVFMAEDPQAFYDSYLTQGQNITIITPMTVREEMANTGKSYANVAYQVDFGQAYE
                     TNTVTNHVPKVTPHKTNTNKEGVSIDGKTVLPNTVNYYKIVLDYSQYKDLVVTEDVLA
                     KGFYMVDDYPEEALILNADGVQVMDKAGNLVNGISVKAYASLSEAPKVVQDAMGKRQF
                     TPKGAIQVLSADDPKAFYEAYVKTGQTLVVTLPMTIKNELT"
BASE COUNT          709 a          473 c          446 g          544 t
ORIGIN      
        1 taaggttgtg ccaaaaacct ttaccccaaa gacttttacc ccagagccac ctgtgagttt
       61 taaggaaaaa ccattagaga aagtcactca gcctagccta acacttacca aggtaacctt
      121 gcctaaagac cctacgccag agccgttacc aaaagcacca caagtgccaa cggttcatta
      181 ccacgactat cgtctgacaa cgactcctga aattgttaag gaagttatca atactgacca
      241 agtgaacctt catgacaaaa cggtcgcaaa agattcaacg gtcatttacc ctttaaccgt
      301 agacgctttt tctccaaatc gagccaaaac cacaagcctc acctttgaag attaccttcc
      361 agtaggctat gcctttgata aagaaaaaac gcaggcagaa aacggaaatt ataccctcag
      421 ctttgatgct gctaagaatt ttgtgacctt gaccgcaaag gaagccttgc ttcaagagat
      481 caacaaagac ctcaccaaga cttaccaact ggtggctccg aaactttatg gtaaccttca
      541 aaatgatggg gcaacctatt ccaacagtta taagctcttt atcaataagg gaaccccaaa
      601 tgcctataca gtgacttcta atgttgtgac ggtgcgtacg cctggtgatg gaacaaccac
      661 tagccgtatt gagcctcaaa aacgcaatga aaatgaagac ggtgtggtta ttaacgatac
      721 ggtagtggct ttaggaacga ctaaccatta ccgtttgacc tgggatttag atcaatataa
      781 aggtgatagg tcttctaaag agacgattgc tcgtggtttc ttctttgtag atgattaccc
      841 agaggaagtg ttgaatttag tagacaaggg aacaagtatc accactcctg aaggcaaagc
      901 cgtattaggg attactgtta aggcttatgc gtcgctgtca gaagctccta aagacattca
      961 agataaactc gctcatgcca agatttctcc taaaggggct ttccaagtct ttatgcctaa
     1021 tgataatcag gctttctatg accagtatgt caaaacagga acttctttaa accttctcac
     1081 caaaatgacg gtcaaagata gtctatatgg tcagacgaag acttatcgaa acaaggctta
     1141 ccaagtggat tttggcaatg gctatgaaac cgatgaggtg acgaataccc ttgttagccc
     1201 agcacctaaa aaacaaaacc taaataagga taaggtggac atcaatggaa aaccgatggt
     1261 ggtcggttca caaaaccact ataccttgtc atgggacttg gaccaatacc gtggcattaa
     1321 agctgataag gctcaaatcg caaaaggctt ttactttgtg gatgactatc ccgaagaagc
     1381 tctattacca gatgaagcaa gtatccaact tgtgacaact gacggcaaag cagtgtcagg
     1441 tgtgacggta aaaacttata cgaacttatc agaagctcct aaaaacctac aagcagctct
     1501 ttcaaaacgc aagattgcgc ctaaaggagc cttccaagtc tttatggcag aagatccgca
     1561 agccttttat gattcatatt taacgcaagg tcaaaacatc accatcatca cccctatgac
     1621 tgtccgtgag gagatggcaa atacaggcaa gtcttatgcc aatgtggctt atcaagtaga
     1681 ctttgggcaa gcctatgaaa ccaatacggt aacgaatcac gtgccaaaag taacaccaca
     1741 taaaactaat accaataaag aaggcgtttc tattgatggc aagaccgttc ttccaaatac
     1801 cgtcaattat tataagattg ttcttgatta cagtcagtac aaggatttgg tagtgacgga
     1861 ggatgttctt gccaaaggtt tttacatggt ggatgattac cctgaagaag cgcttatcct
     1921 aaacgcagat ggtgttcaag tgatggataa ggcaggaaat cttgtcaatg ggatttcggt
     1981 gaaagcctat gcctcattgt cagaagcacc taaagtggtt caagatgcta tgggcaaacg
     2041 tcagttcaca ccaaaaggag ccattcaagt tttaagcgct gatgatccaa aagcgttcta
     2101 tgaggcttat gttaaaactg gtcaaacctt agtggtgacc cttccaatga ctattaagaa
     2161 tgagctgaca aa
//
LOCUS       DASFSW010000223         2146 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.224, whole genome shotgun sequence.
ACCESSION   DASFSW010000223 DASFSW010000000
VERSION     DASFSW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.224"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..232
                     /locus_tag="U1099_002121"
     CDS             <1..232
                     /locus_tag="U1099_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868392.1"
                     /translation="KNDTSKHYVELKPYDKNKFGTEEPLLPVLGNVAKSGRCIKGLNK
                     SNISIYKVRTDILGYRHFIKQEGEHPQLKFKK"
     gene            399..1313
                     /gene="cas1"
                     /locus_tag="U1099_002122"
     CDS             399..1313
                     /gene="cas1"
                     /locus_tag="U1099_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEM4868393.1"
                     /translation="MTWRIVHIHQSEKMRLKLDNLVIKKQGDEFTIPLSDISIIVAEG
                     GDTVVTLRLLSALSKYNIALIVCDNHHLPTGIYHSQNGHFRAYKKLQAQLSWAPLQKD
                     KLWQIIIYFKITNQQDVLAMQEKSLSTIQLLSDYREHIELGDRTNREGHAAKVYFNEL
                     FGKQFVRLTQQETDAVNAGLNYGYTIFRAQMARIIAGYGLNALIGIFHKNEYNQFNLA
                     DDLMEPFRQIVDVWVYMNLREADFLTYQHRLELTNLLNAKIRYGKENCSVTVAMDKFV
                     KGFIRCIEDKDTNQFFCPVVSSLEMEKL"
     gene            1310..1633
                     /gene="cas2"
                     /locus_tag="U1099_002123"
     CDS             1310..1633
                     /gene="cas2"
                     /locus_tag="U1099_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEM4868394.1"
                     /translation="MRYDAMRLLCFFDLPMETTQEKRQYRLFRKELIANGFEMLQFSV
                     YYRTCPNRSFATKFYKKLQQSHLPAGNVRLLAVTEKQFSEMVLIVGGKTRQEEAISDR
                     KLVII"
     gene            1630..>2146
                     /locus_tag="U1099_002124"
     CDS             1630..>2146
                     /locus_tag="U1099_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868395.1"
                     /translation="MKWLITHHVLNNIPIHVGQFTQIVGQNYELKYYIWQILIWYFGG
                     KKYSEEDLILFNQEEPVISQDSEAIKRNAFKIISIAEVGDILEQISYKKGTIGFSYLS
                     LKMQNIEIIEELEILNYHLHKIAQKVNSSISLINDEIEYEVGTTDLLPEQILTKQLTP
                     HFKKSRDEIAIE"
BASE COUNT          765 a          305 c          436 g          640 t
ORIGIN      
        1 taaaaatgat acaagcaaac attatgttga attaaagcct tatgataaga ataagtttgg
       61 tacagaggag ccactgttac cagtgctagg aaatgttgcc aaaagcggac gatgtataaa
      121 aggtcttaac aaatcaaata tatctattta taaagtacgg acagatattt taggctatcg
      181 acactttata aaacaagagg gagaacaccc tcaattaaaa tttaaaaaat aattaaaaaa
      241 ggggttgtat tttttaagta aaaagtgtaa actttaactg taagggaagc cttacactgt
      301 tacttaaatc ttgcagagcc tacaaagata aggctttatg ccgaaatcaa gcaccccgtt
      361 tcgtacgggg tgcttttcgt tataaacagg agaacaccat gacttggaga attgtacata
      421 ttcatcaaag tgaaaaaatg cgattaaagc tcgacaactt agtcataaag aaacaagggg
      481 atgagtttac gattccatta agtgatattt ctataattgt tgcagaagga ggggatacag
      541 ttgttactct tcgcctatta agtgcactta gtaagtacaa tattgcttta atagtctgtg
      601 ataaccacca cttacctaca ggtatttatc actctcaaaa tggacatttt agagcttata
      661 aaaagttgca agctcagcta tcatgggcgc cgctacaaaa ggataaatta tggcaaatta
      721 tcatatattt taaaattacg aaccagcaag atgttttggc aatgcaggaa aaatctttgt
      781 ctaccattca gttattatct gattataggg aacatatcga acttggggat aggacgaata
      841 gggaggggca tgccgctaag gtgtatttca atgagctgtt tggcaaacag tttgttcgac
      901 taacacaaca agagactgat gcagtgaatg cggggttgaa ttatggctac acaattttta
      961 gagctcaaat ggcacgaatt atagcaggat atggtttaaa cgctttaata ggaatttttc
     1021 ataaaaatga atacaaccaa ttcaatttag cagatgattt aatggagccg tttcgacaaa
     1081 ttgttgatgt atgggtctat atgaatttga gggaagctga ctttttaaca taccaacatc
     1141 gtttggaatt aacaaatcta ttgaatgcca agattagata tggaaaagaa aattgcagtg
     1201 tcaccgtcgc aatggataag tttgtcaaag ggtttattcg ttgtatcgaa gataaagaca
     1261 caaatcaatt tttctgtcca gtagtttcaa gtctagaaat ggagaaatta tgaggtatga
     1321 tgctatgcgc ctgttatgct tttttgactt gccaatggaa acgactcaag agaaacgtca
     1381 gtatcgcttg tttcgaaaag agttgattgc taatggcttt gaaatgttac agttttctgt
     1441 ctattatcgt acgtgtccga atcggagttt tgcaacgaag ttttataaaa agttacaaca
     1501 aagtcatcta ccagctggta atgtgagatt attagcggtg acggagaaac aattttctga
     1561 gatggtccta attgtaggtg gtaagacaag acaggaagaa gcgataagtg ataggaagtt
     1621 ggtaatcata tgaagtggct aataacacac catgtattaa ataatattcc aattcatgtt
     1681 ggtcaattta ctcaaattgt cggtcaaaac tacgaattaa aatattatat ttggcaaata
     1741 ttgatatggt attttggtgg aaaaaagtac agtgaggaag atttaatact ttttaatcaa
     1801 gaggagcctg tgatttcaca ggacagtgaa gcgataaaaa gaaatgcttt taaaatcatt
     1861 tcaatagcag aggttggaga tatcctagag cagattagct ataaaaaggg aacgattgga
     1921 ttttcatatt tgtcattaaa aatgcagaac atagagatta ttgaagagct tgaaatatta
     1981 aattatcatt tacataagat tgctcaaaaa gtaaattcaa gcatttcgtt gataaatgat
     2041 gaaatcgagt atgaggttgg aacgaccgat ttacttccag agcaaatttt aacaaagcaa
     2101 cttactccgc atttcaaaaa gtcaagagat gagatcgcta ttgaat
//
LOCUS       DASFSW010000224         2105 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.225, whole genome shotgun sequence.
ACCESSION   DASFSW010000224 DASFSW010000000
VERSION     DASFSW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.225"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..519
                     /locus_tag="U1099_002125"
     CDS             <1..519
                     /locus_tag="U1099_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM4868396.1"
                     /translation="IHENKLQKNVKFLGNLDNPWEEVTDRDICVFPSSMETFGLVYVE
                     AILRGLPVILSDNLGHLTAFEIFSAGQIYKLGDIEELVTKLEQVIESFRNFSDRARLD
                     VERLRTLYTPEVAYKEILNSINHLEYTGHQNTNLSHIKELLTLNMHPTRLERYARLIS
                     NMATKIRHKLRL"
     gene            623..1339
                     /locus_tag="U1099_002126"
     CDS             623..1339
                     /locus_tag="U1099_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4868397.1"
                     /translation="MKVLMIIPAYNEEESILQTVQGIIDYRNSVNFQLDYVVINDGST
                     DSTKEILIQNKLNAVHLVQNLGIGGAVQTGYKYALDNDYDVAVQFDGDGQHDVRSLNS
                     LIQPILVGQADMVIGSRFVGETLSEFQTSFMRRFGIGVISNMIKLTTGNRIWDTTSGY
                     RLGNRKVIAQFAKRYPIKYPEPESTVHLLKQNFQVVEAPANMFERAGGVSSITPFKSI
                     RYMVEVCSSILIASLMKEGE"
     gene            1339..1695
                     /locus_tag="U1099_002127"
     CDS             1339..1695
                     /locus_tag="U1099_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEM4868398.1"
                     /translation="MTIQALAMFLASLGFLYFIFRNINKNKILFEHAFMWIVIGFGLI
                     VFALFDVIPIKLAYLFGFGLTSNFLLSVAIFVLLVIGFLHSMALSQQKQQIKNLIQEV
                     SMAKKRISELEEHHAE"
     gene            1685..>2105
                     /locus_tag="U1099_002128"
     CDS             1685..>2105
                     /locus_tag="U1099_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM4868399.1"
                     /translation="MQNNHTFVICAYGDSKYLEECIHSLKQQTLQSQLILYTSTPSEY
                     IEQICQKNAVVMHTAQGGGIGKDWNLALSFVTTPYATIAHQDDIYLPDYCEKIMSQMT
                     EETLIAYSDYQEVKEGVAISLTSNLKIKQLMLRTMAMF"
BASE COUNT          701 a          314 c          411 g          679 t
ORIGIN      
        1 atccatgaaa ataaattaca gaaaaatgtt aaatttttag gtaacttaga taatccttgg
       61 gaagaagtga ctgatagaga tatctgtgtt ttcccctcat ctatggaaac ttttggctta
      121 gtctatgttg aagcaatctt aagagggctt ccagtaattc tatcagataa tttaggacat
      181 ttgacggcat ttgaaatatt ctcagcaggt caaatttata aattgggcga tatagaagag
      241 ttggtgacta agttagagca agtgattgaa tcctttagaa atttttcaga tagggctcgg
      301 cttgatgtgg aacgtttacg tactctatat acaccggaag ttgcctataa agaaatactg
      361 aacagcatca atcacttaga atatactgga caccaaaata caaatttaag tcacataaaa
      421 gagttactga ctttaaatat gcatccaaca agattggagc gttatgcaag attgatcagc
      481 aatatggcga caaaaatcag acataaactt cgactttaat gaagaatagg atagctatgc
      541 taaaagatgt atattatagt ttcttcaaat gagtaagtag aatctaaaaa gttatataga
      601 attctagttt tagagagaaa aaatgaaagt attaatgatt atccctgcct acaatgaaga
      661 agaaagtatc cttcaaactg ttcagggaat tatagattat agaaatagtg tgaatttcca
      721 attggattat gttgtcatta atgatggttc aacagatagc acgaaagaaa ttttgattca
      781 aaataaatta aatgctgttc acctagtaca aaatttaggc attggtggag ctgttcagac
      841 gggatataaa tatgccttgg acaatgacta tgatgtagca gttcaatttg atggtgatgg
      901 tcagcatgac gttcgttcat taaatagctt aattcagcca atcttagttg gtcaggctga
      961 tatggtgatt ggctcacgtt ttgttggtga gaccttgtca gaatttcaga ctagttttat
     1021 gagacgattt gggataggtg ttatttcaaa tatgattaaa ttaacaactg gaaaccgaat
     1081 ttgggatact acatcaggtt atcgtttggg aaatagaaaa gtgattgcac aatttgcaaa
     1141 acgataccca attaaatatc cggaaccgga atcaactgtt catttactaa agcaaaattt
     1201 ccaagtagta gaagcaccgg ccaatatgtt tgaaagagcg ggaggggttt cttcgattac
     1261 tccatttaag tccatccgct acatggtaga agtttgctca tctattttga ttgcttcgct
     1321 tatgaaggag ggagaataat gaccatacaa gcattagcta tgtttcttgc ttcactaggt
     1381 tttttgtatt ttatttttag gaatattaat aaaaataaga tcttattcga acatgccttt
     1441 atgtggattg ttattggctt cggactgatt gtttttgcgc tctttgatgt tattcctatt
     1501 aaattggctt acttattcgg gtttggtctg acttccaatt tcttgttatc tgtggcaatt
     1561 tttgttctct tggtgattgg atttttgcat agtatggctt tatctcaaca aaagcagcag
     1621 attaaaaatt taattcaaga agtatcaatg gctaaaaaaa gaatctctga gttggaggag
     1681 catcatgcag aataaccata catttgttat ctgtgcttat ggagatagta agtatctaga
     1741 agaatgcatc cattcgttaa agcagcaaac acttcaatca caactcatcc tatacactag
     1801 tactccgagt gaatatatag aacaaatttg ccaaaagaat gcagtcgtaa tgcataccgc
     1861 acaaggagga ggtattggaa aagattggaa tttagcactc tcttttgtaa caacccctta
     1921 tgctacaatt gctcatcaag atgatattta tttaccagac tattgtgaaa aaataatgtc
     1981 tcagatgaca gaggaaacgt tgattgctta ttcagattac caggaagtta aggaaggtgt
     2041 ggctatttcc ttaacttcaa atttgaaaat caagcaactc atgttacgga caatggccat
     2101 gtttc
//
LOCUS       DASFSW010000225         2033 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.228, whole genome shotgun sequence.
ACCESSION   DASFSW010000225 DASFSW010000000
VERSION     DASFSW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.228"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(13..777)
                     /locus_tag="U1099_002129"
     CDS             complement(13..777)
                     /locus_tag="U1099_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEM4868400.1"
                     /translation="MEELRTLNISEIHPNPYQPRIHFDEKELLELAQSIKENGLIQPI
                     IVRKSSIIGYELLAGERRLRASQLAGLTTIPAVVKELTDDDLLYQAIIENLQRSNLNP
                     IEEAASYQKLISRGLTHDEVAQIMGKSRPYISNLLRLLNLSSQTKQAVEEGKISQGHA
                     RQLVSFSEEKQAEWVQLILSKDLSVRTLEKLIAANKKKHTKLKQRDQFLKEQEDSLSK
                     TLGTATKIIKKKNGSGEIRINFNDLDEFERIINNFK"
     gene            complement(838..2025)
                     /locus_tag="U1099_002130"
     CDS             complement(838..2025)
                     /locus_tag="U1099_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEM4868401.1"
                     /translation="MPYMKKYLKFAILFVIGFFGGLIGALSASFFQPRVQQANSAITS
                     VSNVQYNNETSTTKAVEKVQNAVVSVINYQKSANNSLGAIFGNIESSDELAVAGEGSG
                     VIYKKDGQYAYIVTNTHVINNAEKIDILLASGEKISGELVGSDTYSDIAVIKISADKV
                     TAVAEFADSDTIKVGETAIAIGSPLGSVYANTVTQGIISSLSRTVTSQSKDGQTISTN
                     AIQTDTAINPGNSGGPLINTQGQVIGITSSKITSSSANSSGVAVEGLGFAIPANDAVA
                     IINQLEKTGQVSRPALGVHMVNLTTLSTSQLEKAGLSNTELTSGVVIVSTQSGLPADG
                     KLETFDVITEIDGEAIQNKSDLQSALYKHQIGDTITVTYYRNNQKQTVDIKLTHSTEE
                     LSE"
BASE COUNT          573 a          387 c          360 g          713 t
ORIGIN      
        1 ttgtaaacaa gtctatttaa aattgttgat aattctttcg aattcatcga ggtcattaaa
       61 gttaatccga atttctccgc tcccgttttt cttcttgata atttttgtag ctgttccaag
      121 agttttactg agtgaatctt cctgttcttt taaaaattgg tcgcgttgtt taagcttagt
      181 gtgttttttc ttatttgcag ctattaattt ttcaagcgta cgcacactta aatcctttga
      241 taaaatgagt tgaacccatt cggcctgctt ttcttctgaa aatgacacca attgtcgcgc
      301 gtgcccttgt gaaatttttc cttcttctac agcttgttta gtctgagatg atagatttag
      361 taggcgcaat aaattactga tatatggtct tgattttccc atgatttgag caacttcatc
      421 atgtgttaac cctctactaa tcaatttttg ataagaggct gcttcttcta tcggatttaa
      481 gttagaacgc tgcagattct ctatgatagc ctgatagagt aaatcatcat cagttagttc
      541 ctttaccact gctggtattg tagtcagtcc agctaattga ctggctcgca accttctttc
      601 tccagctaat aattcgtatc cgataataga agattttctt acaataatcg gttgaattaa
      661 gccattttcc ttaatagatt gagcgagctc aagtagctcc ttttcatcaa aatgaattct
      721 tggctgatag ggattgggat ggatttctga aatatttagt gtacgtaatt cttccatata
      781 tccttattct agcaaaaaaa atgagacttt acaattgtgt aaagtctcat ttgtcaatta
      841 ttcgctaagt tcttctgtag aatgtgtcaa cttaatgtca acagtttgtt tctgattatt
      901 gcggtaataa gttacagtga ttgtatctcc aatttgatgt ttgtagagag cgctctggag
      961 gtcactctta ttttgaatag cttctccgtc aatctcagta ataacatcaa aagtttctaa
     1021 ttttccatct gcaggtagcc cactttgtgt agagacaatt actacaccgg atgttaattc
     1081 cgtatttgat aatccagctt tttctaattg actagttgac aaggtcgtca agttaaccat
     1141 atgaactcca agagcaggtc ggctaacttg tccagttttt tcaagctgat tgataatagc
     1201 tacggcatca tttgcaggaa tagcaaatcc caacccttct acagccacgc ctgagctatt
     1261 tgcagaactt gaggtaattt tgctagaggt aatgcctatc acttgtcctt gggtattgat
     1321 taacggtccg ccagagtttc cagggttgat agctgtatca gtttgaatag cgttagttga
     1381 gattgtttgt ccatcttttg attgtgaagt aactgtccga cttaagctag aaataatacc
     1441 ctgggtaact gtattggcgt agacgctacc tagaggacta ccaattgcga tagcagtttc
     1501 tccaacttta attgtatcgg aatcagcaaa ttcagcaaca gcagtgactt tatctgctga
     1561 tatttttata acagctatat cagaatatgt atcagaacca acaagttcac cgctaatttt
     1621 ttctccagat gctaaaagga tatcaatctt ttctgcgtta ttaataacat gcgtatttgt
     1681 cacaatatag gcatattgac catctttttt gtagataaca ccagatccct ccccagcaac
     1741 ggctagttcg tcagatgatt caatatttcc aaagatagca ccaagactat tgttggctga
     1801 cttttggtaa ttaataacag acacaacagc attttgtact ttctctacag cctttgtagt
     1861 agaagtttca ttattatatt gaacattgct gacactagtg atggcagaat ttgcttgttg
     1921 aacccgtggc tggaagaaag aggctgataa ggccccgata agacccccaa aaaatccaat
     1981 tacaaataaa atcgcaaatt tcaaatattt tttcatataa ggaatccttt cca
//
LOCUS       DASFSW010000226         2029 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.229, whole genome shotgun sequence.
ACCESSION   DASFSW010000226 DASFSW010000000
VERSION     DASFSW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.229"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            198..665
                     /locus_tag="U1099_002131"
     CDS             198..665
                     /locus_tag="U1099_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM4868402.1"
                     /translation="MTIRKARQSDISVLNELLQDILQVHHQVRPDIFKSAGQKFSEAE
                     LVALLENPDKPIFVFELEGQVVGHLFCELSTTTGDVLEPVKTLFIDDLCVANSARGQK
                     IGEQLYEFALSYAKEQGCHNLTLDVWADNAGAVRFYERQGMKPQKFRMEQIIN"
     gene            complement(687..1946)
                     /locus_tag="U1099_002132"
     CDS             complement(687..1946)
                     /locus_tag="U1099_002132"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEM4868403.1"
                     /translation="MSTFSGFYKKSRQERIDILRQNRSLSEDSLDILYKDENLPEAIA
                     GKMAENHLGTFSLPFSVLPELLVDGQTYSVPMVTEEPSVVAAASFGAKIIAKSGGFTT
                     TIHNRIMIGQVALYDISDHSRATQAILDHKESILETANQAHPSIVKRGGGARELTVES
                     KDEFLIVYLQVDVQEAMGANILNNMLEAIKDDLEELSKGQALMGILSNYATESLVTAQ
                     CHIAISSLATSSAIAQETAQKIALASKLAQVDPYRAATHNKGIFNGIDAVVIATGNDW
                     RAVEAGAHAYASRDGQYKGLSTWSIDGEHLVGSITLPLPIASVGGSIGLNPKVAVAFD
                     LLQQPKARQLASIIASVGLCQNFAALRALVTSGIQAGHMKLHAKSLALLAGAEEHEVD
                     QLAQLLRKEKHSNLETAQNLLAKMREH"
BASE COUNT          562 a          409 c          468 g          590 t
ORIGIN      
        1 gttttctaca ctacacattc tcgttttaag acaacaaaaa accagccttg gccagctgat
       61 tttttaggag atttatgaaa aattttagga tacacctata gtatactcga attttcaaat
      121 tcttcatcgg tctgtagacc tattttgtta aaaaatgaac atgtgctata ataataaaaa
      181 ctcaaaagga gagtattatg acaatccgta aagctagaca gtctgatatt tcggtattaa
      241 atgaattgct tcaagatatt ttacaggtgc atcaccaggt acgaccagat atttttaaaa
      301 gtgcaggtca aaaattttct gaggctgaat tagtagcact tttggaaaat ccagataagc
      361 ctatttttgt ctttgagtta gagggacaag tggttgggca tcttttttgt gaattatcta
      421 ccacaacagg cgatgtttta gaaccagtta aaaccttgtt tattgatgat ttgtgtgtag
      481 ccaattcggc gcgtggtcaa aaaattggtg agcaactgta tgaatttgct ctttcctatg
      541 ctaaggagca gggttgccac aatcttacct tggatgtatg ggctgacaat gcaggagctg
      601 tccgttttta tgaacgccaa ggaatgaaac cacaaaagtt ccgtatggag caaataataa
      661 attaaaaaag agggagtccc tcttttttaa tgttctctca tttttgctaa taagttttga
      721 gcagtttcta ggttgctgtg cttttctttc cgcaggagtt gggctagttg atctacttca
      781 tgctcctcgg cgccagcaag tagcgctagg gattttgcgt ggagcttcat gtgaccagcc
      841 tgaatccctg aagtaacaag agcacgcagg gcagcaaagt tctgacagag accgacagaa
      901 gcgatgatag aagctaattg gcgtgccttt ggttgttgga gaaggtcaaa agcaactgct
      961 acctttggat tgagcccgat agagccaccg acggaggcaa ttggaagagg tagggtgatg
     1021 gagccgacca agtgctcgcc atcaatggac caagtagaaa gccccttgta ttgcccatca
     1081 cggctagcat aagcatgggc cccggcttcg actgcccgcc agtcatttcc tgtagcgata
     1141 acgacagcat ctataccatt aaaaatcccc ttgttatgtg tcgcagcacg ataagggtcc
     1201 acttgtgcta gtttgcttgc cagggctatt ttttgagcag tttcttgagc aatggcagag
     1261 ctggtagcca gactagagat ggcaatatgg cattgggcgg taaccagtga ctccgttgca
     1321 tagttggaga ggattcccat caaagcctgt cccttgctga gctcttctaa gtcatcctta
     1381 atagcttcta acatgttatt gaggatattg gctcccatgg cttcttgtac atcaacctga
     1441 agatagacaa tcaaaaactc atccttactc tcaacagtca attctcttgc accgccccct
     1501 cgtttgacaa tggaagggtg tgcttgattg gcagtttcta aaatactctc cttatggtca
     1561 agaatcgcct gtgtcgctct gctatggtcc gaaatatcgt agagagcaac ttgtccaatc
     1621 atgatacggt tgtgaatagt cgttgtaaaa ccgccagatt tagcgatgat tttggctccg
     1681 aaagatgcag ctgcaaccac agaaggttct tcagtaacca tcggtacaga gtaagtttgt
     1741 ccatccacta gaagttctgg aagtacagaa aaaggtaagg aaaacgttcc tagatgattt
     1801 tctgccatct ttcccgcaat tgcttctggc aggttttcgt ctttgtatag aatgtctaag
     1861 ctatcttcgg atagggaacg attctgacgg agaatatcaa tgcgctcttg gcgtgatttt
     1921 ttataaaatc cggaaaaagt tgacataggt tcctctttct ataggaaagc gccccttggt
     1981 gagagccaaa gggcgccgtt ttggggagcg ggaaagaact tgactgata
//
LOCUS       DASFSW010000227         2022 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.230, whole genome shotgun sequence.
ACCESSION   DASFSW010000227 DASFSW010000000
VERSION     DASFSW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.230"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..321
                     /locus_tag="U1099_002133"
     CDS             <1..321
                     /locus_tag="U1099_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868404.1"
                     /translation="ARQDLINGKISTWNDGLIISTESVEINEETVRLVTERFPDLELQ
                     LENIEVGQQFSWKQNGIYYTFSCLSREDKGIIPLSEITDAVAMQCAEELVDNWLNKET
                     DSLK"
     gene            543..1301
                     /locus_tag="U1099_002134"
     CDS             543..1301
                     /locus_tag="U1099_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4868405.1"
                     /translation="MKSNKEQKYFWLTFSLIVALMIWWFLSNYDRTSRSFPNIILVWN
                     ALLIMIDKGFIFEDIFSSLTSVFFGFTSGFLLSVPNALFMSWYSPYRNIVYPWIQYIR
                     NIPPLAYVPLVVISAGVGLLPQVIVIAIATFLTMTVTIYQGIINIDPNLIKAARILGA
                     SDFEIFKRILVPASIPFIMTAVRLGTSISLTTIIAAESTGATSGMGMRIRSLNNSFDS
                     SGMLLYIILIGVIGLLLNKLVDLIERKLTGWQQK"
     gene            1286..>2022
                     /locus_tag="U1099_002135"
     CDS             1286..>2022
                     /locus_tag="U1099_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868406.1"
                     /translation="MATEVKKIKVKIEEVSKSFQSIHGEVVALNQINLEIFENEIVTV
                     VGPSGCGKSTLLNLVAGLEPVTSGKIYCDDNEIVGVGVERGVVFQQYALFPWMSVQQN
                     VMFGPEMQGKSKLEASEIANKYIKMVGLQEFSNHYPKELSGGMKQRVAIARAYAVNPT
                     ILLMDEPFGALDAQTRIQLQDELIEMLEHERKTCLFITHDIEEAILLGNRVIIMSSRP
                     GEIKEIVNVDLPFPRSQSIKFENDFID"
BASE COUNT          669 a          265 c          395 g          693 t
ORIGIN      
        1 gctcgtcaag acctaataaa tggaaaaatc tctacttgga atgatggatt aattatttct
       61 acagaatctg ttgaaataaa tgaagaaact gttagattgg tcactgagag atttcctgat
      121 ttagaattgc aattagagaa tatcgaggtg ggccaacagt tttcatggaa gcaaaatggt
      181 atttattata ctttctcttg tctttctagg gaagataagg gaattattcc actttctgaa
      241 attactgatg cagttgctat gcaatgtgcc gaggaacttg ttgataattg gttaaataaa
      301 gaaactgact ctttaaaata gtccataatt attgacaatc taaagttaac aaaaatttta
      361 tgttaatttc aaaggaattg ggaatattcg tactgaaaaa ctgatttcaa ttataatgta
      421 agtgtcttag ttaaaacggt aatttttgta gctatactaa ttaatagttt gcaaaatagg
      481 attattttaa tttattaggg ctaaacattt tttaaagtat ttgtaactat gggaggataa
      541 agatgaagag caacaaagaa caaaaatatt tttggttgac attctcattg attgttgcat
      601 tgatgatttg gtggttttta tcgaattatg atcgaacctc tcgtagtttt ccaaatatca
      661 ttttggtttg gaatgcttta ttaatcatga tagacaaagg gtttatcttt gaagatatat
      721 tttcaagtct aacttcagtt ttcttcgggt tcacgtcggg ctttctattg tcggtgccta
      781 atgcattgtt tatgtcctgg tattctccat atcgtaacat tgtatatcct tggatacaat
      841 atatcagaaa tatcccacca cttgcgtatg ttcctttagt agtaatttcg gctggggtcg
      901 ggctgttacc tcaagttatt gtaatagcga tagctacttt tctgactatg actgtaacaa
      961 tttatcaagg cattattaat attgatccaa atttaattaa agcagcaaga attttaggtg
     1021 catcagattt tgaaatattt aaaagaattt tggttccagc atccattcca tttattatga
     1081 cagctgtcag attaggtaca tcgatttctt taactacaat cattgcagct gaatctactg
     1141 gtgctacatc aggtatggga atgagaatac ggtctttaaa taactctttt gattcctcag
     1201 gaatgctttt gtatatcata ttgattggag taattggttt actgttaaat aagttagttg
     1261 atttgataga aaggaagtta actggatggc aacagaagta aaaaaaatta aggtcaaaat
     1321 tgaagaagtt tcaaaatcat ttcagtcaat tcatggtgag gttgttgcac taaatcaaat
     1381 aaatttagaa atctttgaga atgaaattgt tacagttgtt ggtccatcag gttgtggtaa
     1441 atctactcta ctaaacttgg ttgcaggttt ggaacctgta acttccggga aaatttattg
     1501 tgatgataat gaaattgttg gcgttggagt tgagcgtggt gttgtttttc aacaatatgc
     1561 actatttcct tggatgagtg tacaacagaa tgtaatgttc ggacctgaaa tgcaaggaaa
     1621 atcaaagtta gaagctagcg aaatagctaa caagtatatt aaaatggttg gattacaaga
     1681 attttccaat cattatccga aagaattatc gggaggaatg aagcaaagag ttgcaatagc
     1741 aagagcgtat gctgttaatc cgacgattct attaatggat gaaccatttg gagccttaga
     1801 tgcccaaacg aggattcaac tgcaagatga attgattgaa atgctggaac atgaacgaaa
     1861 aacatgtttg tttatcacac atgatattga ggaagcaata cttctaggaa atcgagtaat
     1921 tattatgagt tcaaggccgg gagaaattaa agaaattgtg aatgttgatt taccgtttcc
     1981 aagaagtcaa tccataaaat ttgaaaatga ttttatagat at
//
LOCUS       DASFSW010000228         1989 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.231, whole genome shotgun sequence.
ACCESSION   DASFSW010000228 DASFSW010000000
VERSION     DASFSW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.231"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(172..1551)
                     /locus_tag="U1099_002136"
     CDS             complement(172..1551)
                     /locus_tag="U1099_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEM4868407.1"
                     /translation="MAVIGDVVNISVKQFTRTKKKVLDENGEKVEIDAIVSDSPRNVL
                     LAMKSDNKLNDFLRHNEFTGEHEIVADVKLDAISMRKGQLPSAFESYLSVYLENHFKV
                     VFKTRALQDGIEAFFAEKTYNPVQEYMEHAYDSWDHKERLNQVFQTWLGAEDSIYVQK
                     IAEMFFVGAVSKVFNPWVKFDYTLDLVGGQGAGKTTFLQKIAVDWYTDSAKDFMDKDN
                     YEIMLKSLIVNDDEMVASRKTTFDELKAFVTKTDLTFRRSYGRRAEKFPKNFVIARTS
                     NKVEYLGDKTGERRFLPILVDAAKQFVKPFDMTDNDVLQLWGEAVAIYKKGFTLTFDE
                     DFEDELAVYKERFTYRDEAENQIYDYLDMLVPEEWESMSVVQQHQYTWAYFNNGVYRN
                     DAGFIYEGVKPQESVSSKQILKNVFDIDVAKGDKLARKIKLIMDNNQDWEYKVKKVNG
                     KAIRGYFRK"
     gene            complement(1535..>1989)
                     /locus_tag="U1099_002137"
     CDS             complement(1535..>1989)
                     /locus_tag="U1099_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEM4868408.1"
                     /translation="AHPNNYVLVAPSKTPKGEYAWDLEKSKEGGTMVTASRALVMAIK
                     QEYLKKNNRSELDDIYYQIRNGAGKRNRTTEVFEMIVKGFGEEGSRNDTAAKFAGTLL
                     ARSVDPNCVLELARIANNNSADPLSDRELSRTVDSMVQKHMRGGGSDW"
BASE COUNT          506 a          513 c          358 g          612 t
ORIGIN      
        1 ccaatattac caagggttta gggcggttac accacttttt tatttttttc taaaaaaaca
       61 cctttaaacc cttgatacga ttgagttttt caacggttac atcattggtg taaccttttg
      121 actaaaagtt acatcaaaac acgttttcgg ttacatcact ttttctgcat attatttccg
      181 gaaatatcct cgaattgctt tgccatttac cttcttaact ttgtactccc aatcctggtt
      241 gttgtccatg atcagcttaa tcttccgagc taatttatct cctttggcaa catcgatgtc
      301 aaaaacattc ttcaggatct gcttcgatga cacactttct tgcggtttga caccttcgta
      361 aataaaaccg gcatcattcc gatacacacc attgttgaaa taagcccatg tgtactgatg
      421 ctgttgaact acggacatgc tttcccattc ttccggaacc agcatatcca aataatcata
      481 gatttggttt tctgcctcat cgcgataagt gaagcgttct ttatagactg ccagctcatc
      541 ctcgaagtct tcgtcaaagg tcaacgtgaa gccttttttg taaattgcaa ctgcctcacc
      601 ccacaactgc aggacatcat tgtccgtcat gtcgaatggc ttgacgaatt gtttagctgc
      661 atccaccaag atcggtagga aacgacgctc accagtttta tcaccaagat actcgacctt
      721 gttgctggtc cgagcaatca cgaagttttt gggaaacttt tcagcacgac gaccatagga
      781 cctacggaat gtcaaatcgg tcttggtcac aaaagccttg agctcatcaa acgtcgtctt
      841 cctactagcc accatctcgt cgtcattgac aatcagggat tttagcatga tctcgtagtt
      901 gtccttgtcc atgaaatcct tagcagaatc cgtgtaccag tcaactgcga tcttttgcag
      961 gaatgttgtc ttaccagcac cctgcccacc gaccaggtct agagtgtagt caaactttac
     1021 ccatggattg aagaccttgg acacagcccc gacgaaaaac atctccgcta ttttctggac
     1081 atagatgctg tcctcagcac ctagccaggt ctggaagact tgattcagac gttccttgtg
     1141 gtcccaactg tcataggcat gttccatgta ttcctgaaca ggattatagg ttttttctgc
     1201 aaagaatgcc tcaataccat cttgcaaagc cctggtcttg aacacaacct tgaaatgatt
     1261 ttccaggtaa acactgagat aagattcaaa tgcggagggc agctgacctt tcctcatgct
     1321 gatagcatcc aatttgacat cggccacaat ctcatgttct ccagtaaatt cattgtgccg
     1381 gaggaagtca ttgagcttgt tatcactctt catggccaac agcacgtttc gaggactgtc
     1441 tgacacaatg gcatcaatct caaccttttc tccgttttca tccaagacct ttttcttcgt
     1501 cctagtaaac tgcttgacag agatatttac aacatcacca atcactgcca cctcccctca
     1561 tgtgtttctg taccatacta tctactgtcc gactaagctc tctgtcactc agcggatcag
     1621 cagagttatt attcgcaatc cgtgccaatt ccaaaacaca gtttggatca acacttctgg
     1681 ccaataatgt tccagcaaac ttggctgccg tatcgtttcg actaccttcc tcaccaaagc
     1741 ctttgacaat catttcaaag acctccgtgg tccgatttcg cttgcctgca ccattccgaa
     1801 tttgataata aatgtcatca agttcgcttc ggttgttctt ttttagatac tcctgcttaa
     1861 tggccatgac tagtgctcga ctagcagtga ccatggtccc gccttctttc gacttttcca
     1921 agtcccaggc atactcccct ttgggtgttt ttgatggagc gaccagaaca taattatttg
     1981 gatgtgctt
//
LOCUS       DASFSW010000229         1988 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.232, whole genome shotgun sequence.
ACCESSION   DASFSW010000229 DASFSW010000000
VERSION     DASFSW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.232"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..431)
                     /locus_tag="U1099_002138"
     CDS             complement(<1..431)
                     /locus_tag="U1099_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM4868409.1"
                     /translation="MKRITANHYQTSERYYKLPKLLFESERYKDMKLEVKVAYAVLKD
                     RLELSLSRGWIDEDGAIYLVYSNSKLMALLGCSKSKLLTIKKTLREYGLIDEVQQSSS
                     EKGRLANKIYLGELEHEPIPVSNSDGGGVQKTLGGYQNQPGP"
     gene            complement(428..601)
                     /locus_tag="U1099_002139"
     CDS             complement(428..601)
                     /locus_tag="U1099_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868410.1"
                     /translation="MQKNEQSSRQIVMCHLMAIMGIDIEKATWIVGEMEESGLIQFDD
                     MGNVGLLVLEGQS"
     gene            complement(861..1226)
                     /gene="rplL"
                     /locus_tag="U1099_002140"
     CDS             complement(861..1226)
                     /gene="rplL"
                     /locus_tag="U1099_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEM4868411.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAVAAA
                     GGAAEEAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPAMVKEGVATA
                     EAEEIKAKLEEAGASVTLK"
     gene            complement(1292..1786)
                     /locus_tag="U1099_002141"
     CDS             complement(1292..1786)
                     /locus_tag="U1099_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEM4868412.1"
                     /translation="MSEAIIAKKAELVNAVAEQMKAATSIVVVDARGLTVEQDTVLRR
                     NLRGAEVEYKVIKNSILRRAAEQAGLEGLAELFVGPSAVAFSNDAVAPAKTIYDFAKT
                     ADALEIKGGAVEGKVSSKEEIEALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAEK
                     EDAA"
     misc_feature    complement(1813..1947)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          568 a          458 c          336 g          626 t
ORIGIN      
        1 ggccccggct gattttgata cccccctagt gttttttgaa caccaccccc gtctgaattt
       61 gagactggga taggttcatg ttccaactcc cccaagtaaa tcttattggc caagcgtcct
      121 ttttcactgg aagattgttg gacttcatct atcaaaccat actctcgtaa ggttttctta
      181 atcgtcaata attttgactt ggaacagcca agtagtgcca tgagtttgga gttggaatag
      241 accaaataaa tagctccatc ttcatctatc caccctctac ttaatgacaa ttccaaacgg
      301 tcttttagaa cagcataagc aaccttaact tctagtttca tatccttgta gcgttcgctc
      361 tcaaataaga gtttagggag cttataataa cgctctgacg tttgatagtg atttgcggtg
      421 atacgtttca tgattgtccc tccagcacta acagaccaac attccccatg tcatcaaact
      481 ggattaagcc agactcttcc atctcaccaa ctatccatgt cgctttctca atatcgattc
      541 ccatgatagc catgagatga cacatcacaa tttgtcgtga tgactgttca ttcttttgca
      601 tgattttcct cctgaaaata aaaaaagcga gaacacttat tgaaatgttc tcgctcacat
      661 aaaaatatat ttctacaaat acaaatgttg aagaatagca attctgcaca gtatatttat
      721 ataaaaacaa aaatcctgcc aaagattttt tggcaggatt tttggcagga aaccaaatca
      781 aattatcagt ttctttaaat cgctataagc tctaaaagac tggtaaatcg ggcttccgct
      841 ctcaaattgc tatgtgcttc ttatttaaga gtaactgaag cgcctgcttc ttccaattta
      901 gctttgattt cttcagcttc tgcagttgca acgccttctt taaccatagc tggtgcaccg
      961 tcaacaagct ctttagcttc tttaagacca agaccagtga tttcacgtac aactttgata
     1021 acgccaactt ttttgtcgcc agcagatgtc aattcaacgt cgaatgaatc tttagcttct
     1081 tcagcagcac caccagctgc agcaacagct acaggagcag ccgcagttac accaaattct
     1141 tcttcgatag ctttaacaag gtcgttaagc tcaaggatag tagcttcttt aatttcagca
     1201 ataatgtttt caatgttcaa tgccattgtg attttcctcc aatttaggtt tttaaatagt
     1261 ttgtagcact aagcagctac actctgacgc attaagctgc gtcttctttt tctgcaacag
     1321 ctttgacagc gtatgcaacg ttgcgaactg gcgcttgaag tacagaaagc aacatagaaa
     1381 gcatgccttc gcggtttggc aatgtagcaa gtgcttcgat ttcttcttta gaagaaactt
     1441 taccttctac agcaccacct ttgatttcaa gagcatcagc agttttcgcg aaatcgtaaa
     1501 ttgttttagc tggagcaact gcatcgtttg agaatgcaac tgcagatgga cctacaaaca
     1561 attctgcaag accttcaaga ccagcttgct cagcagcacg acgcaagatt gagtttttga
     1621 taactttgta ctcaacttct gcaccacgca agttacgacg aagaactgta tcttgttcta
     1681 ctgtcaaacc acgagcgtct acaacaacga tagatgttgc agctttcatt tgctcagcaa
     1741 cggcatttac taattccgct tttttagcga taatagcttc actcattttt ttacctccgt
     1801 ttttattttg ggctaggcac aaaaaaatcg cacctaaacc tagacacgaa agtacaaata
     1861 cgtttatctc aataatcgtt ttgtgcctcg gtaggatgtt tatgagtcca gctcccctac
     1921 tgtcttaggt cagttttttc aaacctcaat tatcataaca cagtattttc aataatgcaa
     1981 gacttttc
//
LOCUS       DASFSW010000230         1935 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.233, whole genome shotgun sequence.
ACCESSION   DASFSW010000230 DASFSW010000000
VERSION     DASFSW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.233"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..239
                     /locus_tag="U1099_002142"
     CDS             <1..239
                     /locus_tag="U1099_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM4868413.1"
                     /translation="IDEEYVYEASFSQAIAKKSIIDKAKETILLIDSSKFRMSGFFKI
                     NNLKQYKTIISDKLPDESLLTSAEKYEVEWIANN"
     gene            413..766
                     /gene="mobC"
                     /locus_tag="U1099_002143"
     CDS             413..766
                     /gene="mobC"
                     /locus_tag="U1099_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEM4868414.1"
                     /translation="MEKRTRPNQLKIRLSDKELAQVREKFGQSRSKSMRHFVLKSILE
                     TSIYEIDMQPFREIQHLLSKTSTNINQIAKKVNTYSVVYKEDIVTIQNEVQHLSKELH
                     KLQNTLYNRTNQGEI"
     gene            768..>1935
                     /locus_tag="U1099_002144"
     CDS             768..>1935
                     /locus_tag="U1099_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM4868415.1"
                     /translation="MAVTKIHPIKSTLHFAIDYILDRTKTDSQTLLSTNMCFPTTAHL
                     QFLQTRKEHQVNGSVLARHLIQSFLPGEVDQETAHKIGLELCQKILKDEYEYVLTTHI
                     DKGHIHNHIIFNNVNFKNGKCYQSNKRTYHQIRSTSDRLCKKYKLSVIDEHYQTFRKK
                     YKTRGKSHYEWEQSQNGKGWKPYLQFLIDLNISRSNSWEDFLDRMKKSKCDIKFGKHI
                     SFKGKNASRFTRSKTIGPDYTEERLKERIASKPPHELIDTSTFKDQKGLEFWARKHNL
                     KSMAQGLVELRNLGIYTKDDLEMKIESLADKQISIQDEVKTIDEQMADLEDKMESLHI
                     VQQYQLIYQHYQNNKDNQQYIREYQKEIKAYKMAKNQLLAYDKPLNFRELLSQLD"
BASE COUNT          752 a          356 c          317 g          510 t
ORIGIN      
        1 gaattgatga agaatatgtt tatgaagctt ctttcagtca agccattgca aaaaaatcta
       61 ttattgacaa ggctaaagag acaatattac tgattgatag ttcgaaattt cggatgtcag
      121 gatttttcaa aattaacaac ctgaaacaat acaaaactat catttcagac aaattacctg
      181 acgaatcctt attaacctct gccgaaaagt atgaagtaga atggattgcc aataattaaa
      241 ttaaatagaa tcttagctta ccgctctaca atataaaact gttgtagcaa gcacagtaag
      301 tgggtaccca cttacgataa aattaacgaa gcagacgcct ttggctctgc ttttttcgtt
      361 atattttact tgttagggag aaccctaaga ccccgataga aaggaataga caatggaaaa
      421 acgtacccga ccaaaccaat taaaaattcg actttcagac aaggaacttg ctcaagtgcg
      481 agaaaaattt ggacaatctc ggtcaaaatc aatgcggcat tttgttctca agtctatcct
      541 ggaaacttca atctatgaaa ttgatatgca accttttaga gagatacaac atcttctatc
      601 caagacatca actaatatca accaaattgc aaagaaggtc aatacctact cagttgttta
      661 caaggaagac attgtgacaa tacaaaatga agtccagcac ttgagtaagg aactgcataa
      721 actacaaaac actctctaca ataggacaaa tcaaggggag atttgatatg gctgttacta
      781 aaattcaccc aattaagtct actctccatt ttgcaattga ctacatccta gataggacga
      841 aaacagatag ccaaacactt ctttccacaa atatgtgctt cccaactact gctcatttac
      901 agtttttaca gactcgaaaa gaacaccaag taaatggaag tgtcctggct cgacacctta
      961 ttcaatcctt tttaccagga gaagttgacc aagagacggc acataagatt ggcttggaac
     1021 tatgtcaaaa aatcttaaaa gatgaatacg agtatgtctt aactacacac attgacaagg
     1081 gccatattca caaccatatc atttttaata atgtcaattt caagaatgga aagtgttacc
     1141 aaagcaataa acgaacctat caccagattc ggtcaactag tgatcgactt tgcaagaaat
     1201 acaagctatc cgtcattgat gaacattatc agaccttccg taagaaatat aaaactagag
     1261 gaaaatctca ttatgagtgg gaacaaagtc aaaatggcaa gggttggaaa ccatacttgc
     1321 aatttcttat tgacctaaat atttcccgtt caaactcttg ggaagacttc ctagaccgta
     1381 tgaaaaaatc caaatgtgac atcaaatttg gtaaacatat tagtttcaaa ggcaagaatg
     1441 cttcacgatt tacacgatcc aaaacaattg gtcctgacta tacagaagaa agattaaaag
     1501 agagaattgc ttccaaacca cctcatgagc tgattgatac gtctacgttt aaagaccaga
     1561 aaggattaga gttctgggct agaaaacaca acttaaaatc aatggcacaa ggtttggtcg
     1621 aactaagaaa tttaggaatt tacacaaaag atgacttaga gatgaagatt gaatcattag
     1681 ctgataaaca aatttcaatc caagatgaag taaagactat tgatgaacaa atggctgatt
     1741 tggaagacaa aatggaaagt ctacacatag tacaacagta ccaactcatc taccaacatt
     1801 accaaaataa caaagataac caacaatata taagagaata tcagaaagaa ataaaggcat
     1861 ataaaatggc aaaaaatcaa ttattagctt acgataaacc tttaaatttt agagaattat
     1921 tgtcacagtt ggaca
//
LOCUS       DASFSW010000231         1932 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.234, whole genome shotgun sequence.
ACCESSION   DASFSW010000231 DASFSW010000000
VERSION     DASFSW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.234"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..389)
                     /locus_tag="U1099_002145"
     CDS             complement(<1..389)
                     /locus_tag="U1099_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868416.1"
                     /translation="MRKKLIATIAFCVVCLFLYSFVPQSFGTPTIFQTPYKTSVKSEF
                     EALFHKHGIDVSENDFWATSLNGRTPQDILNEKAKLLYIKFNLLEKEARKYGFNKEKL
                     DFKKVDWKLVSDRYIELKQIVIDEKIKT"
     gene            complement(604..>1932)
                     /locus_tag="U1099_002146"
     CDS             complement(604..>1932)
                     /locus_tag="U1099_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEM4868417.1"
                     /translation="TKSLRGLHVVAEDYGVVHDINIRNLYIHDINGNLSSKWNGGIFF
                     DVYGTTIPTKYDGILIENNYLERVDRSGIKLVGSTWANQNLKNNKNVPLNWYPSTNVV
                     VRGNRIEKAGGDSLTVRDTDGALIEYNISADARYQNTGYNAGIWPFQASNTVIQYNES
                     FRTHGVQDGQGLDLDHISNNSVMQYNYSHDNEGGFMLIMNWYEQTSPTVRYNISQNDK
                     DKIFELARGGAQGTAIYNNTIYSDSKLTGRAGVIDMPSTSGGTGVKDIFLFNNIFYFV
                     DGEKMLVEASDAGKYKDNIHFYNNAYVGVEVPNDPRAITEGIELNGIGTGPIDNKSMI
                     ANTGKYLTGQLDGYKIPFNSVLANSGVSKEDAITYFYEKLGIQQQIDFIENGSLNMSP
                     IEAFSLARQTNSIDAIARSYPKIDGVNYDIDFFGESILTDNLSIGAAQVK"
BASE COUNT          618 a          352 c          247 g          715 t
ORIGIN      
        1 gtttttattt tttcatcaat aactatttgt ttcaattcaa tatatcgatc tgatactaac
       61 ttccaatcta cttttttaaa atctaacttt tctttattaa aaccatattt ccgggcttct
      121 ttttctaata aattgaattt tatatatagt aattttgctt tttcatttag aatgtcttga
      181 ggtgttcgac catttaatga tgtagcccaa aaatcatttt ctgagacatc aataccatgt
      241 ttatgaaata atgcttcaaa ttcggatttc acagaggttt tataaggtgt ctgaaaaata
      301 gttggagtac cgaaagattg aggaacaaat gaatagagaa atagacaaac tacgcaaaat
      361 gcaatcgtgg ctatcaattt ttttctcata ttgttacctc cttatccaaa taatttattt
      421 atctcaatta ttattaatat catatctgaa atatttatta tgtaaattta tattacaatt
      481 tattgagaat cagactgtaa ttttgatttt cactttaaaa attcttagac taagtttctt
      541 cattgtgttt taacgaaaaa aatcattcta tttgacttaa tgaaaaataa ctagctgttt
      601 cactcattta acttgagcag cacctataga taaattgtct gttaaaatcg attctccaaa
      661 gaagtcaata tcatagttaa ctccatcaat ttttggatat gagcgagcga ttgcatctat
      721 actattcgtt tgacgagcta aagaaaaggc ttctattggg gacatattta acgacccatt
      781 ttcaataaaa tcaatttgtt gctgaatccc taatttttca tagaaataag taattgcatc
      841 ttcttttgat acgccactat ttgccaatac actgttgaaa ggaattttat acccgtctaa
      901 ttgaccagtt aaatatttcc cggtatttgc gatcattgat ttattatcaa ttggtcccgt
      961 accaatacca tttaattcta tcccttcagt gattgctcta ggatcattag gcacctcgac
     1021 accaacatag gcattattat aaaaatgaat attatcttta tatttacctg catcacttgc
     1081 ttcaacaagc attttctcac catctacaaa gtaaaaaata ttattaaata aaaagatatc
     1141 ttttactcct gttccacctg acgtacttgg catatcaatt acacctgctc gtccagttaa
     1201 tttcgaatca gagtagattg tattattgta aattgcagtt ccttgtgctc ctcctctagc
     1261 caattcaaaa attttgtctt tatcattctg gctgatatta taacgaactg taggagatgt
     1321 ttgttcgtac cagttcatga ttaacataaa cccaccttca ttatcatgac tgtagttata
     1381 ttgcatcact gaattattag aaatatgatc taaatctaaa ccctgaccat cttgaacacc
     1441 atgcgttcta aatgattcat tgtattgaat aactgtattc gaagcttgga aaggccatat
     1501 acctgcattg tacccagtgt tttggtatct agcatcagca gaaatattgt attcaatcaa
     1561 tgcaccatca gtatctctaa cagttagaga atcaccacct gccttctcaa tacgatttcc
     1621 tctaacaact acatttgtag atggatacca atttaacgga acatttttat tatttttcaa
     1681 attttggttt gcccatgttg atccaactaa ttttattccc gacctatcga ccctttctag
     1741 ataattattt tctattaata taccatcgta tttggtaggt atagttgtac cataaacatc
     1801 aaagaaaatt cctccattcc atttcgaact taaattacca tttatgtcat gtatatatag
     1861 atttcgaata tttatatcat gaacgacccc ataatcctca gcgaccacat gtagtccacg
     1921 aagtgatttt gt
//
LOCUS       DASFSW010000232         1918 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.235, whole genome shotgun sequence.
ACCESSION   DASFSW010000232 DASFSW010000000
VERSION     DASFSW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.235"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            70..738
                     /locus_tag="U1099_002147"
     CDS             70..738
                     /locus_tag="U1099_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEM4868418.1"
                     /translation="MTYSQDYLDDLLVRMAYHSSGIEGNTISLPETVSIILESTLPGK
                     HKSIREFYEIENHKQAFQALLIALDNGEELSLGLVKDIHALLMDRLQHDRGQFKSVQN
                     AIIGATFKTASPEETPFLMQQWVDNANYRLGLPLEIAELLEVLADVHIQFERIHPFSD
                     GNGRTGRLILMFQSMLKLGAPVLISKEDRALYMELLAEQDVTGLSQLLKRSLEFEQER
                     KKQF"
     gene            822..1736
                     /locus_tag="U1099_002148"
                     /pseudo
     CDS             822..1736
                     /locus_tag="U1099_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          588 a          336 c          432 g          562 t
ORIGIN      
        1 cagttggatc atctgaaact gttgaaaaaa gcattgttgc agcagatgtt tatatagaaa
       61 gaggtcagta tgacatattc gcaggattat ttagatgacc ttctggtacg aatggcctat
      121 cattccagcg ggattgaggg gaataccatt tctctgcctg agacggtttc gattattttg
      181 gaaagtacgc tacctggcaa gcacaagagt atccgtgaat tttacgagat tgaaaatcac
      241 aaacaagcct tccaagctct gctaattgcc ttagataatg gggaagagct gagtttggga
      301 ctggttaagg atattcatgc cttgttgatg gaccgcctcc agcatgatcg ggggcagttt
      361 aagtcggtac aaaacgctat tattggtgcg accttcaaga ctgcatcgcc agaggaaacg
      421 ccttttctca tgcaacagtg ggtggataat gccaactatc gcttgggttt gcctttggaa
      481 atagctgagc tgttagaggt gctggcagat gtgcatattc agttcgagcg tattcaccca
      541 tttagtgatg ggaacggccg gacaggacgg ctgattctga tgtttcaaag tatgttaaag
      601 ctgggagcgc ccgtactgat tagtaaagaa gatcgagctc tttatatgga gttgttagcg
      661 gaacaagatg tgactggatt atcccaatta ctcaaacgct ccttggagtt tgaacaagaa
      721 cgaaaaaaac agttttaagt cgagttttca gaaagcgact ttcaatcgga actattattt
      781 ttgcctgaaa atacaatgaa aaccttactt tcacaactac ttggaaacag cgtaagttgg
      841 gggaggtggc ggacttttcc ataaaaacaa actcactttc tagagataag ttatcatcgt
      901 atttttatga agttcagaac attcattatg gggatatttt aacaaaatat gatgctattt
      961 tagatgtatg taacaaagaa cttccatcaa ttattggaag tacgatttca gactttgcag
     1021 atgctttact tagtgaagga gatattgttt tcgcggatgc agcagaagac tcgactgttg
     1081 ggaaagctat tgaagttcga aattttaagg gtaagaatgt tgtttctggt ttacatacga
     1141 tagttgctag gccgaaagtt tcttatgccc cctactattt aggttatcta attaattcaa
     1201 ctgcatacca taatcaaatt ttacctttaa tgcaggggac aaaagtgagc tcaattagta
     1261 aggctaattt aaaatccacg acagtagtgt tccccaccct ccctgaacaa gaagccatcg
     1321 gcagcttctt ctccgaccta gatcagctta ttactcttca tcagcgaaaa ttagatgatg
     1381 ttaaagaatt gaagaaggct ttgttacaga aaatgtttcc gaaagggaat ggaaacgatt
     1441 ttcctgagct aagattccca gaatttacgg acgcttggaa acagcgtaag gcaatggaga
     1501 tttttaagtt tgtgtctgac aaagggtatg cagatttacc aattttgtca gcttctcaag
     1561 aacttgggat gattagacga gatgaaatag gaattgacat caaatatgac aaggaagcgg
     1621 tagcgaatta taagcgtgtt ttaccgggtc aatttgtaat tcatcttcga tcatttcaag
     1681 gaggatttgc atggtctgag atagagggac tgacatcacc tgcctataca atattggatt
     1741 ttaaggaaga aaactcgtca aaattttggc gaaatgtgtt aactagtccg aatttcataa
     1801 aaaaacttga aacagttaca tatggaataa gggatggacg gagcataagt tattctgatt
     1861 tttcgacatt aaattttgtc atccccacgc tccccgaaca agaagccatc ggcagctt
//
LOCUS       DASFSW010000233         1918 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.236, whole genome shotgun sequence.
ACCESSION   DASFSW010000233 DASFSW010000000
VERSION     DASFSW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.236"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            19..408
                     /locus_tag="U1099_002149"
                     /pseudo
     CDS             19..408
                     /locus_tag="U1099_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(409..795)
                     /locus_tag="U1099_002150"
     CDS             complement(409..795)
                     /locus_tag="U1099_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868419.1"
                     /translation="MKILWERLFKFVQQCFREKKTAKITMALSIIMAFGISYLLILPA
                     ITLETDSQESVLQSVYNQPTTVEQPVQEEQAESQLAQVQSAGSLFAEHENITVQVNYE
                     EHTFEQPVRLQVLPSEDSTKYIGLVR"
     gene            complement(814..1416)
                     /gene="lepB"
                     /locus_tag="U1099_002151"
     CDS             complement(814..1416)
                     /gene="lepB"
                     /locus_tag="U1099_002151"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM4868420.1"
                     /translation="MATNKNKDYVTISDLPSISIMSEELDKIRYKKRYLETLKNTIFT
                     LVTVVALAVLIAMLWLPVLHIYGSSMSPTLKAGDMVATVKTNTLSTGDMVAFYYNNKV
                     LVKRVVATSGQWVNIDEQGNVYANGKTLDEPYVKDKAYGQTDIKLPYQVPEGQYFVMG
                     DHRSVSIDSRNTAIGSIGEEQLVGKLTFRIWPFHKMGVIK"
     gene            complement(1406..1699)
                     /locus_tag="U1099_002152"
     CDS             complement(1406..1699)
                     /locus_tag="U1099_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEM4868421.1"
                     /translation="MQKTLEKLKRSELFELLVEQAKEIESYQAKLVDLEKRIEYREAV
                     LGEIGSIAEASLAVTRIFEEAQLAANIYLEQVQKVTHKQSDNCEIGRTDEDGD"
BASE COUNT          570 a          404 c          280 g          664 t
ORIGIN      
        1 tattaagaaa aagactagca gactcaaatc atgcctgatt tgactagcca tcgtgcttgt
       61 caattggctt tttcagatgg tcatacgcat gattttaccc tttttaagga gagtattggc
      121 tatagtttgc cccaaaatac ccttgctttc gtagatttag ggtatttagg catcttgaaa
      181 tttcacgaaa atacctttat tcctgctaag cattctaaac atcacctctt aaccgaagag
      241 gatagacggt tgaatcgaga gatagctaaa atccgtattg aagttgaaca ctttaatgcc
      301 aaatttaaaa cctttaaaat catggcacag ccctatcgta atcgtagaaa acggtttgaa
      361 ttaagaactg agttgatttg tggtatcatc aaccatgaga tgatgtagtt accgaacaag
      421 tccaatatat tttgtactat cttctgaggg taaaacctgt aaccgaactg gctgttcaaa
      481 tgtatgctcc tcatagttaa cttgtacagt tatattttca tgttcagcga ataagcttcc
      541 agcagattga acctgtgcta actgtgactc cgcctgttct tcttgaactg gctgttctac
      601 tgttgttggc tgattataga ctgattgcag aacactctcc tgactgtctg tctctaaagt
      661 aatggctggt aaaatcagca aatatgatat tccaaaagcc ataattattg acaacgccat
      721 agttattttt gcagtctttt tctctcgaaa gcattgctga acaaatttaa acaatctttc
      781 ccataatatt ttcatgtttt atatctcctt cctttatttg attactccca ttttatgaaa
      841 tggccatatc cgaaaggtta actttccaac cagttgctcc tcaccaatcg acccaatagc
      901 tgtatttcta gaatcaatag atacactgcg atgatctccc attacaaaat attgaccttc
      961 tggaacctgg tacggtagtt taatatccgt ttgaccatag gccttatctt taacataggg
     1021 ttcgtctaat gtttttccat ttgcataaac attcccctgc tcatctatat ttacccactg
     1081 accagaagtt gctactactc tttttaccaa aaccttatta ttgtagtaga atgcaaccat
     1141 atctccagtt gaaagtgtat ttgtcttcac agttgccacc atatcacctg ctttcagagt
     1201 aggagacatg gaacttccat aaatatgtag aactggcaac cataacatgg ctattaatac
     1261 tgcaagtgca acgacagtaa caagcgtgaa aatagtattt tttaatgtct ctaaataccg
     1321 ctttttatat cgtattttat ccagctcctc tgacattatt gatatactgg gtaaatctga
     1381 tatggtaaca taatccttat ttttattagt cgccatcctc atctgttctc cctatttcac
     1441 aattatcaga ttgtttatgt gtaacttttt gaacttgttc aagataaata tttgcagcca
     1501 attgtgcttc ttcaaaaatc ctagtaactg ctagagacgc ctccgctatc gaaccaattt
     1561 ctcccaaaac cgcttcgcga tactcaattc ttttttccaa atctactaat tttgcttgat
     1621 aagactctat ttcctttgcc tgctcaacca agagttcaaa taattcagaa cgcttcagtt
     1681 tttctaaggt cttttgcatt taacattgct tcttcctctc tcctttttgc ttatattgct
     1741 ttcgctacaa aatatatgct tatttacttc taaatatcac ctatcaatca aaacatatac
     1801 atttttgtga atataatatg atcaaattag cctgtacgtg ataccaaacc aatccttagt
     1861 ttgtcaacaa tgccaatttc ttaaaaaaca caaaaaaatc agaacttttt gcacacat
//
LOCUS       DASFSW010000234         1884 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.237, whole genome shotgun sequence.
ACCESSION   DASFSW010000234 DASFSW010000000
VERSION     DASFSW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.237"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            51..1640
                     /locus_tag="U1099_002153"
     CDS             51..1640
                     /locus_tag="U1099_002153"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEM4868422.1"
                     /translation="MSKTIQAITNQIWSMANELRGNMDASEYKNYILAFMFYRYLSEH
                     QERFLVEDEIISPAPGETVQDAYAREAVGNDLVEHLENTASHLGYAIEPEDTWARLVA
                     KIDNSEIVASDYQTIFDHFNANVELNRDAMEDFRGVFNDINLGDSRLGNSTVARAKSL
                     NSIVKLIDSIEYKNDEGKDILGEIYEYLIGQFAASAGKKGGEFYTPHQVSKILAKIVT
                     LGLEKSDTSFSVYDPTMGSGSLLLTVRNELPQGQHIKFYGQEMNTTTYNLARMNLMMH
                     QVSYSNMILNNADTLESDWPDGVDELGIDQPRSFDAVVANPPYSAKWDNRESKLKDPR
                     FMEYGKLAPASKADFAFILHSLYHLNNTGTMAIVLPHGVLFRGAAEGHIRKLIIEKNY
                     LDAVIGLPANLFYGTGIPTTILVFKKNRQTKDVFFIDASKEFEKGKNQNHLSDDMVEK
                     IVETYHNRQSVDKYAHLASIEEIVENDYNLNIPRYVDTFEEEEEIDLGQVTQQLEQDR
                     LEIRALEDKIRQQLKTLGVDF"
     gene            complement(1691..>1884)
                     /locus_tag="U1099_002154"
     CDS             complement(1691..>1884)
                     /locus_tag="U1099_002154"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="catalyzes a two-step reaction, first charging a
                     serine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEM4868423.1"
                     /translation="RAQIRYRDAADGKVKLLHTLNGSGLAVGRTVAAILENYQNENGS
                     VTIPEVLRPYMGGVEVIKP"
BASE COUNT          583 a          357 c          435 g          509 t
ORIGIN      
        1 aaatgagatt tcgagtaaaa aatgaaacaa catgaaagga ttacaaaaac atgtcaaaaa
       61 ctattcaggc cattactaat cagatttggt ccatggccaa tgaattgcga ggaaatatgg
      121 atgcttcgga atataagaac tatatcctag ccttcatgtt ctatcgctat ctatctgagc
      181 atcaagagcg tttcttggtg gaagatgaaa ttatcagtcc tgctccagga gaaactgttc
      241 aggatgccta tgcgcgagaa gcagttggga atgacttggt agagcatttg gaaaatacag
      301 cttctcacct tgggtatgcc atcgagcctg aagatacctg ggcaagactg gtagccaaaa
      361 ttgataacag tgagattgta gcgagtgact accagacgat tttcgatcat tttaacgcca
      421 atgtagaact caatcgggat gctatggaag atttcagagg cgtctttaat gacatcaatc
      481 taggggactc tcgtttgggc aattcaacgg ttgcgcgtgc aaaatccttg aacagtattg
      541 tgaagttgat tgatagcatt gagtataaaa atgacgaagg aaaggatatt ctgggagaaa
      601 tctatgagta tcttattgga cagtttgccg cttcggcagg taaaaaaggt ggagaatttt
      661 ataccccgca tcaagtgagt aagattttag ccaagattgt tactttaggt ttggaaaaat
      721 cagatactag cttctctgtt tacgatccaa ccatgggttc agggtctttg ctactaacag
      781 tgcgtaatga actacctcag ggccagcata tcaaattcta tggccaagaa atgaacacaa
      841 ctacttataa cttggcgcgg atgaacctga tgatgcatca ggtcagctac agcaatatga
      901 ttttgaataa tgctgatacc ttagaaagtg actggccgga cggggtcgat gaacttggaa
      961 tcgatcagcc acgtagtttt gatgcggtcg tcgcaaaccc accctactct gctaaatggg
     1021 acaatcgtga gtccaaattg aaggatccac ggtttatgga atacggtaag ctagcaccag
     1081 cctctaaagc ggactttgct tttatcttac acagtcttta tcatctgaat aatacaggga
     1141 ccatggccat tgttttacca cacggagtgc tctttagggg agcagcagaa ggtcatattc
     1201 gcaaactgat tattgagaaa aattacctgg atgcagttat tggcttgcca gctaacctgt
     1261 tttatggcac aggcattcca accactattc tggtcttcaa aaaaaatcgt cagaccaagg
     1321 atgtcttctt tatcgatgcg agcaaggagt ttgaaaaagg gaagaaccag aatcaccttt
     1381 ccgatgatat ggtagaaaaa atagtagaga cctatcacaa tcgccagtct gttgacaaat
     1441 atgctcattt ggcatcaata gaagaaattg tagaaaacga ctacaatcta aatattcctc
     1501 gttacgtcga tacttttgag gaagaggaag agattgatct cgggcaagta acgcagcaac
     1561 tagaacaaga tagactagag attcgggctc tagaagataa gattcgtcaa caattaaaga
     1621 ctttgggagt agatttttag ataaatgaaa agagcgagta ggatttttcc tcgctctttc
     1681 tttgtatgtc ttaaggttta atcacttcta ccccacccat gtatggacgg agaacttctg
     1741 gaatggtgac agaaccgttt tcattttgat agttttcaag gattgccgca acggtacgac
     1801 caactgctag accagaaccg ttgagggtgt ggagcaattt gaccttgccg tcagcagcat
     1861 cgcggtagcg gatttgggca cgac
//
LOCUS       DASFSW010000235         1847 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.238, whole genome shotgun sequence.
ACCESSION   DASFSW010000235 DASFSW010000000
VERSION     DASFSW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.238"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..257
                     /gene="ybeY"
                     /locus_tag="U1099_002155"
     CDS             <1..257
                     /gene="ybeY"
                     /locus_tag="U1099_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEM4868424.1"
                     /translation="LLDNPELAEMMEDFDAYIGELYISIDKAREQAEDYGHSYEREMG
                     FLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAYGLTRE"
     gene            238..639
                     /locus_tag="U1099_002156"
     CDS             238..639
                     /locus_tag="U1099_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEM4868425.1"
                     /translation="MDLQENNSKNRWKNRELIASLEFAVTGLLTAFKEERNMKKHLAS
                     AILVVLAGLIFQVSVTEWLFLLLSISLVIAFEIVNSAIENVVDLASDYHFSMLAKNAK
                     DMAAGAVLFVSGFALLTGLIIFVPKIWDFIF"
     gene            656..1555
                     /gene="era"
                     /locus_tag="U1099_002157"
     CDS             656..1555
                     /gene="era"
                     /locus_tag="U1099_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEM4868426.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNYVMGQKIAIMSDKAQTTRNKI
                     MGIYTTEEEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEKRGKGD
                     DMIMERLKQAKVPVILVVNKIDKVHPDQLLEQIDDFRQQMDFKEIVPISATQGNNVNR
                     LMEILKENLDEGFQYFPADQITDHPERFLVSEMIREKVLHLTREEIPHSVAVVIESMK
                     RDEFTDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
BASE COUNT          552 a          348 c          451 g          496 t
ORIGIN      
        1 acttactgga caatcctgaa ttggctgaaa tgatggaaga tttcgatgcc tatattggtg
       61 aattgtatat ttctatcgat aaggcgcgtg agcaagctga agattatggt catagctacg
      121 agcgcgaaat gggcttttta gctgtacacg gttttctgca tatcaatggc tatgatcatt
      181 atacgccaga agaagaagca gaaatgtttg gtttacaaga agaaattttg acggcctatg
      241 gacttacaag agaataattc aaaaaatcgc tggaaaaacc gagagctgat tgccagtcta
      301 gaatttgcag taacgggttt gttgacggct ttcaaggaag aacgcaatat gaagaagcac
      361 cttgcttctg ctattcttgt ggtgcttgcg ggacttattt tccaagtatc tgtgacagag
      421 tggctcttcc tcttgctcag tattagtctg gttatcgctt ttgaaattgt gaattcggct
      481 attgaaaatg tggtggattt ggcttcagac tatcattttt ctatgttggc gaaaaatgcc
      541 aaggacatgg cagcaggggc agtcttgttt gtgtcaggat ttgccttgct aacaggattg
      601 attatctttg tgccgaaaat ttgggacttt attttttaga atataaggag aatacatgac
      661 atttaagtca ggttttgtgg cgattttagg tcgtccaaac gttgggaaat caacctttct
      721 caactatgta atggggcaaa aaattgccat catgagcgac aaggctcaaa caacccgtaa
      781 caagattatg ggcatttata cgacagaaga agaacagatt gtcttcattg acacaccggg
      841 aattcacaag cctaagacag ctttgggcga ttttatggtg gagtctgctt atagcaccct
      901 tcgtgaagtg gacacggtcc tctttatggt gccagccgat gaaaaacgag gcaagggtga
      961 tgacatgatt atggaacggc tcaagcaggc caaggttcca gttattctcg ttgttaacaa
     1021 gatagacaag gttcatccag accagctttt ggaacagatt gatgatttcc gtcaacagat
     1081 ggacttcaag gaaattgtgc caatttctgc tacccaaggg aacaatgtca accgacttat
     1141 ggaaattctc aaggaaaacc tggacgaagg cttccagtat ttcccagcgg accaaatcac
     1201 tgaccaccca gaacgtttct tggtatctga gatgattcgg gagaaggttc tacacctgac
     1261 tcgtgaggaa attccgcact ctgtggccgt tgtcatcgag tccatgaaac gtgatgaatt
     1321 taccgacaag gtccacattc gtgctacgat tatggtagag cgtgatagcc agaaaggcat
     1381 catcatcggt aagcaaggag ccatgctcaa gaaaatcggc tccatggctc gtcgtgatat
     1441 tgaactcatg ctaggtgaca aggtcttcct cgaaacctgg gtcaaggtca agaaaaactg
     1501 gcgagacaaa aaactcgacc tcgccgactt tggttataat gagaaagagt attaaaaacg
     1561 tccgggggag tgaaacagtc taggaataga ctgtttcagc tggtcaccta gaaatgtgaa
     1621 agtgaccatt tgaaaacccg agcatagaaa ttggaaggcg agatagcctg gggataggct
     1681 gtctaagtca atcacttaga aatagttagc agaaaagccg aaaggctttt actgcgttat
     1741 ggagaaatct ttttagtaaa caagaaagga aagtcgatta ggtgttgtct tgaacacgcc
     1801 tacgactctt tgaaaaaaga taaccgtgac ctaggtgccg aagcacc
//
LOCUS       DASFSW010000236         1839 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.239, whole genome shotgun sequence.
ACCESSION   DASFSW010000236 DASFSW010000000
VERSION     DASFSW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.239"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            238..1314
                     /locus_tag="U1099_002158"
     CDS             238..1314
                     /locus_tag="U1099_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEM4868427.1"
                     /translation="MTVKPNITYLYGDKVYTNTNLDFWGNQWDMQKSISTGSKFQHKS
                     SGKATIGVIDSGITYDNPDIYSHIISVQNFTSDLETGIVDNQNVVDKIGHGTSVVGQI
                     SSSGEYLGIAPDMKIRMYRVFDEGNAQDQWILKALIQAAKDDVDVINLSLGEYLLRDS
                     TDEDDDTALINIYQRAINYAHNQGSVVVASVGDEGMDLQNQDELKNYLGSLRGKDYSQ
                     VDGTVQDIPAELDNVVTVGSVDDNDLVSSFSNQGNGDIDIYATGGGSQALSKVGYDKW
                     VADKLYEKEWILVPTLEGKYTYAYGTSISTPKVSAALGLIIEKYNLKDQPDEAIRILY
                     DNCFLSNNGNNGQIRLLNITNFVQ"
     gene            1355..>1839
                     /locus_tag="U1099_002159"
     CDS             1355..>1839
                     /locus_tag="U1099_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4868428.1"
                     /translation="MYKILVVDDDFEIVKLMRTILEMKNYNVTTYQEVRLPIKISDFD
                     GFDLILLDVMMPNVDGLQICKQIRKSVSSPIIFVSAKDSEDDIVSGLNLGGDDYITKP
                     FSVNQLVAKVAAHLKREERYRNAESNESVIRELFPLTFYLQEKLVCVNGEAISLTKRE
                     Y"
BASE COUNT          661 a          217 c          367 g          594 t
ORIGIN      
        1 agcggttctg tgtcatcagg agtaagtgct aatgaaaata aggaaattag ttataatgtt
       61 ttagttagtg aaaataaatc tattgaggat gtagaagatg ttttacaaag tcaaggaatt
      121 aaagttactg ataaaatacc tgaaataaat tttttaacta tttctaccga aaaaagtgaa
      181 gatacaataa aaaaagaaaa ctctaattat attgatgaca tagttataga taatactatg
      241 actgtaaagc ctaatattac ttatttgtat ggggacaagg tttatacaaa tactaattta
      301 gatttttggg gaaatcagtg ggatatgcaa aagagtatta gtactggaag taaatttcaa
      361 cataagtctt ctggtaaagc aacaattggt gtaatagatt ctggtattac atatgataat
      421 ccagatattt attcacacat tatttcagta caaaatttca catcagattt ggaaacagga
      481 atagttgata accaaaatgt tgtagataaa attggccatg ggacctctgt tgtgggacaa
      541 atttcatcaa gtggagaata tttagggata gcacctgaca tgaaaatacg aatgtatcgt
      601 gtttttgatg aagggaatgc tcaagaccaa tggattctca aagcgctcat tcaagcagca
      661 aaagatgatg ttgatgttat taaccttagt ttaggagaat atttgttaag agattccact
      721 gatgaagatg atgatacagc tttgatcaat atctatcaac gggcaattaa ttatgctcat
      781 aatcaaggtt ccgttgtcgt tgcttcagta ggtgatgaag ggatggattt acagaatcaa
      841 gatgaattaa aaaactatct tggtagttta agaggaaaag attatagtca agttgatgga
      901 actgtacaag atattccggc tgaattagac aatgttgtta cagttggttc agtagatgat
      961 aatgatcttg tatctagttt ctcaaatcaa gggaatggcg acattgatat ctatgcaaca
     1021 ggaggtggaa gtcaagcgct ttctaaagta ggatatgata aatgggtagc tgataaatta
     1081 tatgaaaaag aatggattct tgttccaacg cttgaaggaa agtatacata tgcatatggg
     1141 acttcaattt cgactcctaa agtctctgca gctttaggat tgattataga aaagtataat
     1201 ttgaaggacc aaccagatga agctattcga attttatatg ataattgttt tcttagtaat
     1261 aatggaaaca atggacaaat taggctacta aatattacaa attttgtgca ataatagtgt
     1321 aaaatagtag tatgagataa aagaggtacc tagtatgtat aagattttgg tagttgatga
     1381 tgattttgaa attgtaaaat tgatgcgaac aattttagaa atgaaaaact ataatgtgac
     1441 gacttatcaa gaagttaggc ttcctataaa aataagtgat tttgatggat ttgatttaat
     1501 attgcttgat gttatgatgc caaatgttga tggtctgcaa atctgtaaac agattcgtaa
     1561 atcagtatcc tctccgatta tttttgtcag tgcaaaagat tctgaagatg atattgtatc
     1621 tggcttaaat ctcggtggtg acgattatat cacaaagcca tttagtgtta atcaactagt
     1681 tgctaaggtt gcagcgcatt taaaacgaga agaacgttat agaaatgctg aatctaatga
     1741 atctgttatt cgagaattat ttccactaac attttacctt caagaaaaac tcgtgtgtgt
     1801 taatggtgaa gctatttctc ttactaaaag ggaatatga
//
LOCUS       DASFSW010000237         1829 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.240, whole genome shotgun sequence.
ACCESSION   DASFSW010000237 DASFSW010000000
VERSION     DASFSW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.240"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>1829
                     /locus_tag="U1099_002160"
     CDS             <1..>1829
                     /locus_tag="U1099_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEM4868429.1"
                     /translation="VRLVKSLESEHRQATPSEQELLAKYVGWGGLANEFFDEYNQKFS
                     KEREELKTLVTEKEYSDMKQSSLTAYYTDPLLIREMWNKLERDGFTGGRVLDPSMGTG
                     NFFAAMPKHLRENSELYGVELDTITGAIAKHLHPNSHIEVKGFETIAFNDNSFDLVLS
                     NVPFANIRIADSRYDKPYMIHDYFVKKSLDLVHDGGQVAIISSTGTMDKRTENILQDI
                     RETTVFLGGVRLPDSAFKAIAGTTVTTDMLFFQKHMDKGYVADDLAFSGSIRYDKDDR
                     IWLNPYFDGEYNSQVLGSYEVRNFNGGTLSVRGTSDHLLADVQTALKQVKAPRVVDNY
                     DIFITPDVIKRQVIDTSVPPEIRESLDQYSFGYKDSTVYYRDNKGIRVGTKTEEISYY
                     VDDEGTFKAWDTKHSQKQIDRFNALEVTDSTALDVYVTEEATKRGQFMGYFKKTVFYE
                     APLSEKEVARIKGMVDIRNAYQEVIAIQRYYDYDRDEFNHLLGHLNRTYDTFVKRFGY
                     VNSAVNRNLFDSDDKYSLLASLEDESLDSSGKTVIYTKSLAFEKALVRPEKEVTAVSS
                     ALDALNSSLADGRGVDLAYMMSIYQTDSKATLIEELGDAIIPDP"
BASE COUNT          529 a          339 c          433 g          528 t
ORIGIN      
        1 gttcgtttgg tgaaaagtct tgaatctgaa caccgtcaag ccacacctag tgaacaagaa
       61 ctccttgcta aatacgtggg ctggggaggc ttagctaacg agttctttga cgagtacaac
      121 caaaagtttt ctaaggaacg tgaggaatta aaaacgctag tcacagagaa agagtattct
      181 gacatgaaac agtcctctct gaccgcttac tacacagacc ctctcttgat tcgtgagatg
      241 tggaataaac tagaacgaga tggctttaca ggtggaagag tgctagatcc ttcgatggga
      301 acgggaaatt tctttgcggc aatgcctaaa cacctgagag aaaatagtga gttgtatggt
      361 gtagagctag acacgattac aggggctatt gccaaacacc ttcatccaaa tagccatatt
      421 gaagtaaagg gctttgagac cattgccttt aatgacaata gctttgattt ggttctctca
      481 aatgtgcctt ttgccaatat caggattgcg gatagtcgct atgacaaacc ctatatgatt
      541 catgattact ttgtcaaaaa atcattagat ttggtgcatg atggtgggca agtcgctatc
      601 atttcctcca caggaaccat ggataaacgg acagaaaata ttctccagga cattcgtgag
      661 acgactgttt ttcttggtgg tgtgcgttta ccagatagcg cctttaaggc cattgcagga
      721 acgactgtca caacagacat gttgttcttc cagaaacata tggataaggg atatgtggca
      781 gatgatttag ctttttcagg ttctattcgc tatgacaagg atgaccgtat ttggttgaat
      841 ccttactttg atggagaata caacagtcag gttcttggga gctatgaggt tagaaacttt
      901 aacggaggga cactttctgt gagaggaact tctgatcatt tgctagcaga tgttcagact
      961 gctcttaaac aggttaaagc ccctagagtg gtggataact atgatatctt catcactcct
     1021 gatgtgataa aaagacaagt cattgatacc tctgttccgc ctgaaattag agaaagtcta
     1081 gaccaatata gttttggtta taaagattct acggtttact accgagataa taagggaatc
     1141 cgtgttggga ctaagaccga ggagattagc tattatgtgg atgatgaggg gacctttaag
     1201 gcttgggaca ctaagcattc tcaaaagcaa attgaccgtt ttaatgcttt agaagtgact
     1261 gatagcaccg ctcttgatgt gtatgtgacc gaagaagcca ccaaacgtgg gcagtttatg
     1321 ggctatttca aaaagacggt tttctatgaa gctcccttgt cagagaaaga agtggcacgg
     1381 attaaaggga tggttgatat tcggaacgcc taccaagagg tgattgccat tcagcgctac
     1441 tatgattacg acagagatga gtttaaccac ttgttaggac atctcaatcg cacctatgat
     1501 acttttgtga aacgctttgg ctatgtgaat agtgctgtga accgtaacct ctttgatagt
     1561 gatgataagt attcccttct tgcgagttta gaagatgaaa gtcttgactc aagtgggaag
     1621 acagttatct ataccaagtc gcttgccttt gaaaaagccc tagttcgtcc tgaaaaggaa
     1681 gtgacggcag tttcttctgc ccttgatgct ctcaattcta gtctagcaga tggtcgtggg
     1741 gtcgatttgg cttatatgat gtctatttat cagacggatt ctaaagcgac tttgattgag
     1801 gaattaggtg acgctattat tccagaccc
//
LOCUS       DASFSW010000238         1825 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.241, whole genome shotgun sequence.
ACCESSION   DASFSW010000238 DASFSW010000000
VERSION     DASFSW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.241"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..585
                     /locus_tag="U1099_002161"
     CDS             <1..585
                     /locus_tag="U1099_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM4868430.1"
                     /translation="YWFHHGHLGLTKADMLYPRKGKRVKKQASPNFKPAGKSIEERPD
                     VINLRLENGHYQIDTVLLTRGKNHCLLVLTDRRSRHQIIRLIPNKTAESVNQALEGIL
                     REHRILSITVDNGTEFSRLAEVLPEEYIYYAHPYSSWERGTNENHNRLIRRWLPKGTK
                     KTTPKEVAFIEHWINNYPKKCLDYKSPSEFLMGG"
     gene            complement(944..1816)
                     /locus_tag="U1099_002162"
                     /pseudo
     CDS             complement(944..1816)
                     /locus_tag="U1099_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          569 a          300 c          366 g          590 t
ORIGIN      
        1 tattggtttc atcatggcca tttaggattg acgaaagcag atatgcttta ccctagaaaa
       61 gggaaaaggg tcaagaagca agctagtcct aactttaaac cagcaggaaa atctatcgaa
      121 gaacgtcctg acgttattaa tcttcgcttg gaaaacggtc attatcaaat tgatacagtt
      181 ctactaacca gagggaaaaa tcattgcctc ttagtcttaa ccgaccgacg aagcaggcac
      241 caaatcatac gattaattcc aaacaaaacc gccgaatcgg tcaatcaggc actggaggga
      301 atcttgaggg aacatcgtat cctgtctata actgtagata atggtacaga atttagtcgt
      361 ctggctgaag tgttacctga ggagtatatc tactatgcgc atccctactc ttcatgggag
      421 aggggaacga atgaaaatca caatcgattg attcggagat ggttacctaa aggaaccaag
      481 aaaacgactc caaaagaagt cgcttttatc gaacattgga tcaacaacta ccctaaaaaa
      541 tgcttggact acaagtcgcc gagtgaattt cttatgggtg gctaatttca acttgaaatt
      601 tggggtctaa gctacttata acatttgaaa ccttaattta taagtttgag ttgaggattg
      661 tggatttcaa gcagctgttt tcttctaaaa aaataacaaa atcgaaacgg tccatatcca
      721 ctttgagtgg tagttggcta tttttgattt taaatgagta tcacacccca tcgtcccatt
      781 gactgttccc caatctcgcc gctttgagaa gaaaggcaga aatgatattc tgatgaaaat
      841 tcgtctcgga aaagtggaag tcacattctt tcactctatc aaccaagaaa cattagaaac
      901 aatcttagat aaggtactat tttatgacca tccgactcag tgattaggtc aagtctactt
      961 ggtttgcggg aagtagtggt gtgatttggt taagaggatt atgctcactt agaatttcta
     1021 cctgcttgct ttgagagaca tgagtgtaga tttgtgttac attgatgtta ctgtggccca
     1081 gtaaatgttg tatttgtcga atatcgacgc cattatcaag caacatagtt gcaaaactat
     1141 gtcgaaacat atgtggagtg atatgttttt tcagttgtag cgagtgagaa atatttttca
     1201 gacttaagcg cacactttgt tctttaagat gatcatcgtt tttcacactg gtaaatagat
     1261 aactagaatg actacaatga tgcgctatat agtcttcaag taacgaaagt gtttcatcgt
     1321 ttccaatata gagtagtcgc actttctttc ctttttcgag tatttgaatg gttctatttt
     1381 ccaaattgat attatcaagt tttaaattac ataattcaga gatgcgtaga ccagttgata
     1441 aaagtagcga gataaggagt agatttcgca tagctttccg cttagaatgc ttatttttag
     1501 aattttctat ttgttggtgg gcgaaacaat atattttttg taaatctttc aaggaaatcg
     1561 ttttaggaag ctttttctct tccttgtagt ggagtcgaag ctgatggaag ggattttttg
     1621 aaattagttt ttcttcttcc agaaatttat agtatgattt aagactagca atcttacgtt
     1681 ttaatgtgct agtctttttt atatgttggt tgagaaaagt aagataggac ctaatatcat
     1741 tggtcctaaa gctataatac taatataaat tggatttgta tgcttggata gtatgagaac
     1801 taagtcgtct ttgaatagca caaaa
//
LOCUS       DASFSW010000239         1819 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.242, whole genome shotgun sequence.
ACCESSION   DASFSW010000239 DASFSW010000000
VERSION     DASFSW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.242"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1691
                     /locus_tag="U1099_002163"
     CDS             <1..1691
                     /locus_tag="U1099_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM4868431.1"
                     /translation="KAPYQVYTRSSLPGGDGYTTYSNATRSIAAVGNVFPVVLYLVAA
                     LVTFTTMARFVDEERTQSGLFKALGYTNRQIMAKFILYGLTAGLVGTIVGIIAGNLLL
                     SPLISNIITQTTVIGPAKLHFYPLWTGLALLLSLASSVLPAYLVARRELTEKPAQLLL
                     PKPPVTGSSIWLEKWPAIWSRLSFTHKVTARNIFRYKLRMLMTIFGVAGTVALLFGGL
                     GIRSSISGVVQRQFGELIHYDMLVVENSRATEEELDKLTHFLQSDQVRQSLPVAFEQL
                     NQTVEEDGQRKNLSISLYISDRRDFGNQVSLESSTGQPIKLSDRGIVLTEKLAQIYGV
                     SVGDKLSLTLEDKEVSVRVEAVADMYAGHFIYMTDSYYEQVTGKQKTANAYLVQLKDS
                     QLGHIQTLASQLLAMPAVRSLVQNTSLIDMLTTIAGSLQTIMTILVILSILLGLVILY
                     NLTIINMSERIRELSTIKVLGFHNKEVTMYIYRETIVLSLIGMLVGLVGGIYLHKLLL
                     AMIGSDSIRFNPSVGLEVYLIPILAISGILAVLGWYVNHHLRKVDMLEALKSVD"
BASE COUNT          466 a          407 c          425 g          521 t
ORIGIN      
        1 aaaaagcgcc ctatcaggtc tatactcgat caagcctacc aggtggagat ggctatacta
       61 cctatagcaa tgcgactagg tctattgctg cagtgggcaa tgtcttccca gttgttctct
      121 accttgtcgc agctctagtg acttttacca ccatggcccg ttttgtcgat gaagagcgaa
      181 cacagtcagg tctcttcaag gcccttggct atacgaatag acagattatg gctaagttca
      241 ttctctatgg tttgacggca ggtctggtag gaacaatagt cggtataata gcaggcaatc
      301 tcctcctgtc tccacttatt tccaacatta ttacgcagac aaccgtgatt ggaccagcta
      361 aacttcattt ttatcccctt tggacaggtt tagccttgct cctgtctttg gcttcctcgg
      421 tcctgccagc ttacttggtg gctcgtcggg agctgacaga aaaacctgct cagctacttt
      481 tacctaagcc acctgttaca ggctccagca tttggcttga aaaatggccg gctatctggt
      541 cccgcctgag cttcacccat aaggtgacag cccgcaatat cttccgctac aagcttcgta
      601 tgcttatgac catctttgga gtagcaggga ccgtcgccct tctttttgga ggactgggta
      661 tccgttcctc catctcagga gttgtccaac gacagtttgg ggagctgatc cattatgata
      721 tgttggtggt ggaaaacagc agagctactg aggaagagtt ggataagtta acacattttc
      781 ttcagtcaga tcaggttcgc caatccttgc cagtggcttt tgaacagctc aatcaaacgg
      841 tggaagaaga cggtcagaga aagaatttat cgattagtct ctacatttca gaccgtcggg
      901 attttggaaa tcaagtcagt ctggaaagtt cgacaggtca gcctattaaa ctgagtgaca
      961 gggggattgt cctcacagaa aaattggcgc aaatctacgg ggtctcagtt ggtgataagt
     1021 tatcactgac cttggaagat aaggaagttt cagtcagggt agaggctgtg gctgatatgt
     1081 acgcagggca ttttatctat atgacagata gctactacga acaagtgacc ggcaagcaga
     1141 agactgccaa tgcctatttg gttcagttga aagacagtca gttggggcat attcagactc
     1201 ttgcaagtca actcctagcg atgccagcag tcagaagttt ggttcaaaat acttctctta
     1261 tagatatgct taccacgatt gcaggctcgc ttcagaccat catgaccatt ctagtcatcc
     1321 tatccatctt gcttggcttg gttattcttt acaaccttac aattatcaat atgtctgaac
     1381 ggattcgtga attatccacc atcaaggtac tgggcttcca caataaagag gtgaccatgt
     1441 acatctatcg cgaaaccatt gtcctttcgc tgattggcat gttggttgga ctggttggtg
     1501 gtatttatct ccacaagctc cttcttgcca tgattggttc ggacagtatc cgcttcaatc
     1561 catctgtcgg actagaagtc tatctcattc ctatcttagc cattagtgga attttggctg
     1621 ttcttggttg gtacgtcaat caccatctga gaaaggtgga tatgctggaa gccttgaagt
     1681 cagttgatta aaacagtctg ggaatagact gttttaaccc gagccttgaa atcggagagc
     1741 gaggcacagt cttggtttgg aaggaatttt aaaaagagaa tgacagtctt gcgattcaaa
     1801 agaggctgag caaaaagcc
//
LOCUS       DASFSW010000240         1818 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.243, whole genome shotgun sequence.
ACCESSION   DASFSW010000240 DASFSW010000000
VERSION     DASFSW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.243"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            37..255
                     /locus_tag="U1099_002164"
     CDS             37..255
                     /locus_tag="U1099_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEM4868432.1"
                     /translation="MKRLYDVQQLLKRFGIIVYMGNRLYDIEMMQIELNRIYQAGVLD
                     RLEYMEAELVLRREHRLELEYQKSRENE"
     gene            255..641
                     /locus_tag="U1099_002165"
     CDS             255..641
                     /locus_tag="U1099_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM4868433.1"
                     /translation="METFWVFLVFLAVLGAWMGFNYWRLRRAATVIDNAEFAEKIHGG
                     QLIDLRDPSEFNRKHILGARNIPYQQLRQSTAALRKDRPVLIYENDRGQLVTPAALHL
                     KKQGFNEIYILSYGLNGWNGKVKTTK"
     gene            698..1420
                     /locus_tag="U1099_002166"
     CDS             698..1420
                     /locus_tag="U1099_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEM4868434.1"
                     /translation="MEKKFEKLSLAEIGIISFMGLILLISLGASWYKNAQSETSDTKT
                     VQQVPNQKKTVDTLFQDAENAVIVLEGTHTEEKLAEAQSAVNKVTDATKKAALQKRID
                     QVKELVGQQTDAQTQAKLKAELVRAEAAQKVADEKKVSETGTSQSTDLAEASETSQES
                     YVVPNQNIYGNQSVSSTSQYTSSVASTSTSASVPPTSEGAPVVETPTVSETPAETTDS
                     TTSLVESVETVPTADESSPDNP"
     gene            1719..>1818
                     /locus_tag="U1099_002167"
     CDS             1719..>1818
                     /locus_tag="U1099_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEM4868435.1"
                     /translation="MRIVSGSYGGRPLKTLDGKTTRPTSDKVRGAMF"
BASE COUNT          604 a          290 c          434 g          490 t
ORIGIN      
        1 attgaaaaac acctaaaatt ctgctacaat ttttttatga aaagacttta tgatgtgcaa
       61 caattactca aacggtttgg tataattgtc tatatgggca accgtctcta cgatattgaa
      121 atgatgcaga tagaattaaa tagaatctat caggcaggtg tcttagatcg gttggaatat
      181 atggaagcag agcttgtttt gaggcgtgaa catcgtttgg aattagagta tcagaaatca
      241 agggagaatg agtgatggaa acattttggg tatttttagt atttttggca gtattgggag
      301 cctggatggg ttttaattac tggagattgc gtcgtgcagc gactgtgatt gataatgcag
      361 aatttgcaga aaagatacac ggaggtcagt tgattgacct gcgagacccg agtgagttta
      421 atcgtaagca tatcttaggt gcgagaaata ttccttacca gcaattgcgc cagtctacag
      481 ctgctttgcg taaggacaga cctgtcttaa tctatgagaa tgatcgtggt cagttggtaa
      541 cgcctgcagc tcttcatctg aagaaacagg gctttaacga aatctatatt ttaagctatg
      601 gtctaaacgg atggaatggt aaggtaaaaa caactaagta gcattgtcag taggctagag
      661 tattttctta tgcaatgaga cggataaggg aaaaaatatg gaaaagaaat ttgaaaagtt
      721 gtcattggca gaaattggca ttatttcttt tatgggactt attttattga ttagcttagg
      781 cgctagctgg tataaaaatg ctcaaagcga aacaagtgat acaaagacag tacagcaagt
      841 gcccaatcag aaaaaaacgg ttgatacatt atttcaggat gctgagaatg ctgtcattgt
      901 attagaagga acacatactg aagagaaact tgcagaggca caatctgcag taaacaaggt
      961 tacggatgcg actaaaaaag cagcattaca gaaacggatt gaccaagtaa aagagctagt
     1021 gggacagcag acagatgctc aaacacaggc taagctaaaa gcggagttgg tcagggcgga
     1081 agcagcccaa aaagtagctg atgagaaaaa agtatctgaa acagggacta gtcagtcaac
     1141 ggaccttgct gaagctagtg agacctcaca agaatcctat gtagtgccta atcaaaatat
     1201 atatggaaat caaagtgtga gttcaacaag ccagtataca agcagcgtgg cgtcaactag
     1261 tacaagtgct tcagtccctc cgactagtga aggagcacct gtggtagaaa ctccaacagt
     1321 ttctgaaacc ccggcggaaa cgacggattc aacaactagc ctggttgagt cagtggaaac
     1381 agttccaaca gctgacgaga gttcgccgga taatccgtag gctttttagc tacggggtgt
     1441 attagcatac tggaaaaaat agctatttgt atttcaacat caattttttc ttgtaacgat
     1501 ctagtttaaa tacatacgat aagagtgagg aatttcgcgt aaaacagaat ttccttgctt
     1561 tttattttta tcattttatt gaataatgtg agaatgattt cctgctatag cggttgagag
     1621 tgtatttgat aggaggatga aaaagttggt gcaaaaagga aagcaggaag tgcacttggt
     1681 ttcagcaacg agcttcattt tttgctataa tataccttat gagaattgta tcgggaagct
     1741 atggtggaag accactgaaa acgttagatg gaaaaacaac cagaccgaca tcggataagg
     1801 tccgtggagc catgttca
//
LOCUS       DASFSW010000241         1782 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.244, whole genome shotgun sequence.
ACCESSION   DASFSW010000241 DASFSW010000000
VERSION     DASFSW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.244"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(105..419)
                     /locus_tag="U1099_002168"
     CDS             complement(105..419)
                     /locus_tag="U1099_002168"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEM4868436.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAKPTQEFTSV
                     KSTLRALEEIESGNVVIHPDPEAKREAVRRRAEEAKRLAEEEERKIKAQIAKEKEEGE
                     KI"
     gene            complement(443..1069)
                     /gene="gmk"
                     /locus_tag="U1099_002169"
     CDS             complement(443..1069)
                     /gene="gmk"
                     /locus_tag="U1099_002169"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEM4868437.1"
                     /translation="MKERGLLIVFSGPSGVGKGTVRKEIFESSDNKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRSREEFEDLIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDGVFIFLTPPDLEELQDRLIGRGTDSEEVIAQRIERAKEEIALM
                     REYDYAVVNDEVPLAAERVKRIIEAEHFRVDRVIGRYDEMIREQIAER"
     gene            complement(1217..>1782)
                     /locus_tag="U1099_002170"
     CDS             complement(1217..>1782)
                     /locus_tag="U1099_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEM4868438.1"
                     /translation="QNVLRHSIEVAKLAGVLAAELGENVNLARRAGFLHDVGKAIDRE
                     VDGSHVEIGTELAKKYKENPIVINAIASHHGDVEATSTIAVIVAAADALSAARPGSRR
                     ESMEAYIKRLQDLEEIANSFEGIKQSYAIQAGREIRIIVHPNKVTDDQITILAHDVRE
                     KIENNLDYPGNIKITVIRETRATDVAK"
BASE COUNT          484 a          445 c          306 g          547 t
ORIGIN      
        1 aatattaaaa gataaactaa atgacctgcc tcctttctgt caaaactttc atcattccga
       61 aagtgaaaag aaacccggat ggcaatccca accgagtctt gtttctaaat cttttcgcct
      121 tcttcttttt ctttagcaat ttgagctttg attttacgct cttcttcttc cgccaaacgt
      181 ttagcctctt cggcacggcg acgaacagcc tcacgtttcg cttctgggtc tgggtgaatg
      241 actacattac ctgattcaat ttcttccaag gcgcgtaggg tggatttcac tgaggtgaat
      301 tcttgagttg gtttggcacc tgcttccaat tcatgggcac gcttagcctc aagaataacc
      361 aaagaatact tagatggtac cttatccaac aaggtatcaa ttgacggctt caacatcata
      421 atgcgattct ctcttctttc ttttatcgtt ctgctatctg ttcacgaatc atttcatcgt
      481 agcgaccaat cactcggtct acacggaaat gttctgcttc gataatacgc ttgacacgct
      541 cagcagccaa cgggacttca tcgttcacaa ctgcataatc atactcacgc atcaaggcga
      601 tctcttcctt ggcacgttcg atacgttgag caataacttc ctcactatcc gttccgcgac
      661 caatcaagcg atcttgcaac tcctccaagt caggtggtgt caagaaaatg aacaccccat
      721 ctggaacctt tttcttcact tggagggcac cctgtacttc aatttctaaa aagacatcaa
      781 ttcccttatc aagagtttca ttgacatagg tcagaggtgt tccatagtaa tttccaacgt
      841 actctgcata ttccaacatt tgaccctggc gaatcaaatc ttcaaattcc tcacgactac
      901 ggaagaaata gtcaactcca tcaacttctc ccggtctctg tggacgtgtc gtcatggata
      961 cagaatattc aaatttatta tcagagcttt caaaaatttc ctttcgaact gttccttttc
     1021 caacaccaga tgggccagaa aagacaatga gtaagcctcg ctctttcatg atatctcctt
     1081 ttaaatcttt cattctagtg taacaaaatt tagcatgaat ttcaactgct tttaaatacg
     1141 attagaatat tttctattcc caaccctaaa cgaactttga gccagtagaa aaagagccct
     1201 tcggactctt ctacatttac ttagcaacat ctgtcgctct tgtttcacgg ataactgtga
     1261 ttttgatatt tcctggataa tccagattat tttcaatttt ctcacgaaca tcatgggcca
     1321 agatggtaat ctgatcatca gtgaccttat taggatgaac gataatccga atttcacgac
     1381 cagcctgaat agcataggat tgtttaatcc cttcaaaact gttggcgatt tcttccaagt
     1441 cttgcagacg tttgatgtag gcttccatag attcacgacg cgaacctgga cgagcagcgc
     1501 tcagagcatc tgccgcagcc acaatgactg caatagtact tgtcgcttct acatcgccat
     1561 ggtgactagc aatcgcattg ataacaattg gattctcttt atatttctta gccaattctg
     1621 taccgatttc aacgtggctt ccatccacct cacggtcaat agctttacca acgtcatgta
     1681 ggaaaccagc tcgccgtgcc aaattcacat tctcgcccaa ttcagcagca agtacacctg
     1741 ctaatttagc aacttcgatt gagtggcgaa gaacattttg tc
//
LOCUS       DASFSW010000242         1775 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.245, whole genome shotgun sequence.
ACCESSION   DASFSW010000242 DASFSW010000000
VERSION     DASFSW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.245"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1026)
                     /locus_tag="U1099_002171"
     CDS             complement(<1..1026)
                     /locus_tag="U1099_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3552 domain-containing protein"
                     /protein_id="HEM4868439.1"
                     /translation="MEIITVVVTLVSALIGLVLGYFAISLKMKSAKETAELTLLNAEQ
                     EASNVRGRAEEQAEVILKTAERDRQTLKKELLLEAKGEARKYREEIAEEFKSERQELK
                     QIESRLTERATSLDRKDDNLTNKEKTLEQKEQSLTDKAKHIDEREQEVVKLEEQKALE
                     LERVAALSQEEAKEIILTDTRDKLTHEIATRIKEAEREVKERSDKMAKDLLAQAMQRI
                     SGEYVAEQTITSVHLPDDAMKGRIIGREGRNIRTFESLTGIDVIIDDTPEVVVLSGFD
                     PIRREIARMTMETLLQDGRIHPARIEELVEKHRVEMDNRIREYGEAAAYEVGAPNLHP
                     DLIKIMGR"
     gene            1154..1636
                     /locus_tag="U1099_002172"
     CDS             1154..1636
                     /locus_tag="U1099_002172"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEM4868440.1"
                     /translation="MKKEVTVESFELDHTIVKAPYIRLISEEVGPKGDIITNFDIRLI
                     QPNENAMDTAGLHTIEHLLAKLIRQRIDGLIDCSPFGCRTGFHMIMWGKQDSEKIAQV
                     IKSSLEEIAEGITWEDVPGTTIESCGNYKDHSLHSAKEWAKLILSQGISTDAFERKPI
                     "
BASE COUNT          513 a          413 c          311 g          538 t
ORIGIN      
        1 acgtcccatg attttaatca agtctggatg aaggtttggt gccccaactt cataagcagc
       61 agcttcccca tactcacgaa tacggttatc catctctaca cggtgtttct caaccaactc
      121 ctcaatacga gctggatgaa tacgaccatc ttgaagcaag gtctccatgg tcatacgggc
      181 aatctcacgg cgaatcggat caaaacctga tagaacaacc acttctggtg tatcatcaat
      241 gataacatcg atacctgtga gactttcaaa ggtacgaata tttcgaccct cacgtccaat
      301 aatccgtccc ttcatcgcat catccggtaa atgaaccgat gtaatcgtct gctcagccac
      361 atattcacct gatatgcgtt gcatcgcttg ggctaacaag tcctttgcca tcttgtcaga
      421 acgctctttt acttcacgct ctgcttcctt gatacgagtc gcaatttcat gcgtcagttt
      481 atcgcgtgta tcggtaagga taatttcctt cgcctcttct tgactgaggg ccgccactcg
      541 ttctaactcc aaagcttttt gttcttcgag ttttacaact tcttgttcac gctcatcaat
      601 gtgcttagct ttatcggtca gactttgttc cttttgttcc aaagtctttt ccttgttggt
      661 caaattatcg tctttgcggt ccaagctggt cgcacgctct gtcaagcgtg attcgatttg
      721 cttcaactct tgacgttctg acttaaattc ttctgcaatt tcttctcggt acttacgagc
      781 ttctccctta gcttccaaga gtaattcttt tttcaaggtc tggcgatcgc gctcagctgt
      841 ttttagaata acctcagctt gctcttctgc tcgaccacga acattactag cttcttgttc
      901 tgcgtttaaa agtgtcaatt ctgcagtttc ttttgcagat ttcatcttca aagaaatggc
      961 aaagtaacct aatactaaac caatgagagc agaaacaaga gtcactacaa ctgtaatgat
     1021 ttccatattc tacctctttt ttataattcg attttcacaa aatctgtatc tattttatca
     1081 tatttgaaac gatttcacaa ttccaaattg tcaaatatgg tataataaat tcatcactta
     1141 tttggaggaa accatgaaaa aagaagtcac tgtcgaaagc ttcgagctag accacactat
     1201 tgtaaaagca ccttacatcc gcttaatttc tgaagaagtc ggaccaaaag gagacattat
     1261 tacaaacttt gatattcgcc ttatccaacc caacgaaaat gcaatggata cagctggtct
     1321 acataccatt gagcatctat tagctaaact aattcgccag cgcatcgatg ggctaatcga
     1381 ctgctctcct ttcgggtgtc ggacaggttt ccacatgatt atgtggggaa aacaagactc
     1441 tgaaaagatt gctcaagtca tcaagtcgag tttggaagaa attgcagaag gcatcacttg
     1501 ggaggatgtc ccaggaacaa ccattgaatc atgcggtaac tacaaagacc acagccttca
     1561 ttcagctaaa gaatgggcca aactaatcct ttctcaggga atctctacag atgcctttga
     1621 aagaaaacca atctaacaaa aaaacggata caccgctcag aacgtagaca aaatcctaaa
     1681 atataaaaaa ttgaagaagt ttacgaaaga tagcaagtca ttaaactctt agaaacattg
     1741 atatttcggc gtttctgaga gtttttaatt ttcaa
//
LOCUS       DASFSW010000243         1741 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.246, whole genome shotgun sequence.
ACCESSION   DASFSW010000243 DASFSW010000000
VERSION     DASFSW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.246"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..931
                     /locus_tag="U1099_002173"
     CDS             <1..931
                     /locus_tag="U1099_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM4868441.1"
                     /translation="QKVQAVTYKTALPHHKLFIQYFGSKPLKGITARDCESFRLHIIE
                     HYSENYAKNLWSRFKACMGYAERLGYISSMPCKSLDNPRGKHPDTPFWTYIEFQNFIK
                     SFDLQDYEELQRFTTIWLYYMTGVRVSEGLSLWWEDVDFEHKFLKVHTTLEKNENGNW
                     YRKDQTKTPAGERLIELDDVTISVLENWRKNQVANQDTDFIISRFGEPFCKSTICRII
                     KRKAKEVGVPVITGKGLRHSHASYLINVLQKDILYVARRMGHADKSTTLNTYSHWFNA
                     LDKTVSEEITQNVISAGLDSILCQNSCQTLSKG"
     gene            1192..>1741
                     /locus_tag="U1099_002174"
     CDS             1192..>1741
                     /locus_tag="U1099_002174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM4868442.1"
                     /translation="MNALLFDERPIVANPVLAREIGLNEAIVLQQINFWLEINKQNGK
                     NYHDGKYWTYNSIRAWHESDFSYLSYETVKRTFSKLEKAGYLFTGNYNKDPRDKTKWY
                     SLNYDKIFTLEEEVKQKRNNVASKVLEKADENDVTNALGQNDPMEKFKLIQCNNSERT
                     NGLVQTEPMQSVNMSQPLPNNTS"
BASE COUNT          618 a          271 c          340 g          512 t
ORIGIN      
        1 gcaaaaggta caagctgtaa cttataaaac tgctttgcca catcataagt tatttattca
       61 gtattttggc tctaaacctt taaaaggcat aacagctaga gattgtgaat cttttagatt
      121 gcacatcatt gagcattatt ctgaaaatta tgcaaagaat ttatggtcac gatttaaggc
      181 ttgtatggga tatgctgaga gattaggata tatttcatct atgccatgca agtcgttaga
      241 taaccctaga ggcaagcacc ctgatactcc attttggact tatattgagt ttcagaactt
      301 tattaaatca tttgatttac aagattacga agagttacaa agattcacta ctatttggtt
      361 atattatatg acaggtgttc gtgtaagtga aggtttatcg ttatggtggg aagatgtcga
      421 ttttgaacat aaattcttga aagttcatac aaccttagag aagaatgaaa atggaaattg
      481 gtatcgtaaa gaccaaacca aaacacctgc tggagaacgc ctcattgaac tggatgatgt
      541 gacaatatca gtattggaaa attggcgaaa aaatcaagtt gccaatcaag atacagattt
      601 cattatttca cgatttggtg aacccttttg taaatcaacg atctgtcgaa ttattaagag
      661 aaaagctaaa gaagttggag taccagtgat aacaggaaaa ggattgcggc acagtcatgc
      721 ttcctatctc ataaatgttt tgcagaagga cattttatat gtggccagac gaatgggaca
      781 tgctgataag tctacaactt tgaataccta cagtcactgg tttaatgcat tagataaaac
      841 agtatcagaa gaaatcaccc aaaatgtaat tagtgcagga ctagattcca ttttatgcca
      901 aaattcctgc caaactctga gtaaaggcta aaaagcctat tataccgcca acagagtaac
      961 gtgtgttatt gttactctta aataatatat tagagtatat ttggagtgag aaaccaagta
     1021 ataaggcttt taacgccttt atggtgattt aaattggttt ggaatcattt gattccccac
     1081 cacaaacccc accacgatta aacgtggtgg ggttttataa tattttagat tattgagcaa
     1141 tcagaaaatt ctggttgctt ttctttttga agaatgacgt aggaggtgtg tatgaacgca
     1201 ctgttgtttg atgagcgacc aatagtcgca aatcctgtct tggccagaga aattggattg
     1261 aacgaggcta tcgtccttca acaaattaac ttttggctag aaataaataa acagaatggg
     1321 aagaactacc atgatggaaa atactggact tataattcta ttcgggcttg gcatgagtcc
     1381 gatttttctt acctaagtta tgaaactgta aaacggacat tttcaaaact agaaaaagcc
     1441 ggctatttat ttacggggaa ttacaataaa gatccaaggg ataaaacaaa atggtattca
     1501 ttgaactatg acaaaatttt cacattagag gaggaggtaa agcaaaagag aaataacgta
     1561 gcaagtaaag ttctagaaaa ggcagatgag aatgatgtta ccaatgcatt gggtcaaaat
     1621 gacccaatgg aaaagttcaa attgatccaa tgcaataatt cagaaagaac taatggattg
     1681 gttcaaactg aaccaatgca aagtgtcaat atgagccaac cattaccaaa taatacttca
     1741 a
//
LOCUS       DASFSW010000244         1740 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.247, whole genome shotgun sequence.
ACCESSION   DASFSW010000244 DASFSW010000000
VERSION     DASFSW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.247"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>1740
                     /locus_tag="U1099_002175"
     CDS             <1..>1740
                     /locus_tag="U1099_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEM4868443.1"
                     /translation="FPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDG
                     TYSSERLASECKALKILNEWSETPKIYWCGKIWEHTFLGVEHMKGVPLNRWVTNNFPL
                     YEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQ
                     AVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQ
                     TRIEGKKLFESLGYIREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQ
                     DIQDFIIKLLRGFTLTYGQWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAK
                     KVKNFKFDDYFEIRTQVINEAINNLKKASFSGLFDGKIGTIWLIYEFGEIDRAVELFT
                     THFIEIFENSQNKNLYSGQAGILLVGLYFLSKGEIDNKLGEEILVRLREYTLNYIENP
                     ENFCKVGASDVQSNDPYENFGGLLYGHAGVAWLFGEAYKLTGESIYKDGLELAIDKEL
                     VAYKVDSNNSLQYSQGHRLLPYLATGSAGLLLLINRNKEILSSNYLKYLTSLERATDV
                     VFCVLPGLFNGFCG"
BASE COUNT          613 a          211 c          353 g          563 t
ORIGIN      
        1 ctttcccttt attatagata atgcaattag attttcaaac agtggtggga tatataatgc
       61 taggctaaaa gaagatggga aaaaaataat tttaaaagag gctagaccgt atacaggtct
      121 aggatttgat ggtacttatt cttcagagag attagcttcg gagtgtaaag cactaaaaat
      181 tctaaatgaa tggagtgaga cacctaaaat atactggtgt ggtaagatct gggagcacac
      241 cttcttagga gttgaacata tgaagggcgt tccactaaat agatgggtta ccaataattt
      301 tcctttatat gaagttgttg ataagacaaa agattatcta ttgagagttt caaagatagt
      361 agaaaaactc atcgatttaa cgaataagtt tcattctgaa aatgtatatc atcaagattt
      421 acatttagga aatatcttgg ttaaggatga agatgagatt agtataatag actgggaaca
      481 ggctgttttt tcgaatgatg aaaaagttgt acataaagtt gcagcccctg gttttagagc
      541 atggcgagaa acacttccca gtgagataga ttggtatggt attagacaaa tagctcatta
      601 tttatacatg ccactagtaa ctacatcgga tttgacatac aactatgtaa gtcaaactag
      661 gatagaagga aaaaaactat ttgagtcttt gggttatata agagaacata tagattatgt
      721 agaaagttta ttgtcatatc tagattctaa atgtccacag atcgagaata tttccaggaa
      781 aaaagtatta aaacccatgc atgagattag aactatcgag agtgagcaag atatacaaga
      841 ttttattata aaattattga gaggatttac gctaacttat ggacagtggc gtaaagaatt
      901 tcaatcaaga ttcttcccgg ttcattatta tggtctgaat tttaatcaag gaattgcgtt
      961 ttcagattta gctatccttt ggagttatca acaactagct aagaaagtaa aaaatttcaa
     1021 gtttgatgac tattttgaga ttcgaacaca ggttattaat gaggcaataa ataatttaaa
     1081 aaaagcgtcc ttttctggtc tttttgatgg taaaattggc acaatctggc tcatttatga
     1141 atttggggag atagatcggg cagttgaact ttttacaact cattttatag agatatttga
     1201 gaattcacaa aataaaaatt tatatagtgg acaggctgga attttactgg ttggactgta
     1261 ttttcttagt aaaggggaaa ttgataataa actaggagaa gaaatactag ttaggcttag
     1321 agaatatact ttaaattata tagaaaatcc agaaaatttt tgtaaagttg gcgctagtga
     1381 tgttcagagt aatgatcctt atgagaattt tggtggatta ctatatggtc atgctggggt
     1441 agcttggctc ttcggtgaag cttataaatt aactggtgaa agtatctata aagatggtct
     1501 agaattggct atagataaag aattagttgc ttataaagtg gatagtaata attcattgca
     1561 gtacagccaa ggtcatagat tactaccata tctagcaact ggaagtgcgg gcctactttt
     1621 acttattaat agaaataaag aaatcttgtc aagcaactac ttaaaatatt taacttcatt
     1681 agagagggca actgatgtag ttttttgtgt attaccaggt ttatttaacg gattttgtgg
//
LOCUS       DASFSW010000245         1713 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.248, whole genome shotgun sequence.
ACCESSION   DASFSW010000245 DASFSW010000000
VERSION     DASFSW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.248"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(3..1580)
                     /locus_tag="U1099_002176"
     CDS             complement(3..1580)
                     /locus_tag="U1099_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868444.1"
                     /translation="MKKRNFWLTLTCIFAVALLNVGFLYQYRYIIDAIAAGDRQAFVS
                     YFVLMGLTVLVMLVFEYIRQIANVSYLNQVGFQLQATFIGKIFDLPFRQFVEQGAGAY
                     ISQLNNDLETVKEDYYDAFFTIFQGACTFGIASLALLSLDGLTAIFLILISFLPVVIP
                     YLFKKRRRLNQEAISDSQQVYNTRVSDVFLSYLQVKNSHQRQQVLDGLNDRYQAVNDK
                     VNQATRTTVTMRLLVGFVFYLTTLSIIFIGGFQVFSGALTIGGLTAILTISEQLVDPI
                     NSIAAAFLDRHAVKKLKQEFELSTFGQETKGQELASAFQSIQLVKASYAVGEKQVFED
                     LTVAFERGKKYLILGESGSGKSSLALILTKNSQLQAGDIYVDKTSLSDLSYQAIQDKI
                     AYLPQEGSLFHDTVLYNLTMGREIPEDRLMFLIKTMNLDSRFPSYASLSEEISDDSGL
                     SGGQKQRLLLIRALLQDKDILLLDESLSALDQETYAVIENYLTSLADKTLIHISHRMS
                     EEVLSRYDGVVRIGESN"
BASE COUNT          542 a          398 c          329 g          444 t
ORIGIN      
        1 tattaattgc tctccccaat ccgtaccacc ccatcataac ggctaaggac ctcctcggac
       61 atgcggtggg aaatatgaat gagcgtctta tcagctagtg aggtaaggta attttcaatc
      121 actgcatagg tttcctgatc caaggcagac agactttcat ccagcaacaa gatatcctta
      181 tcttgcagca aggcacggat caagagcagg cgttgctttt gtccgccaga cagtccgcta
      241 tcatcactaa tttcctcgct caaagaagca taacttggga aacgactgtc aaggttcatg
      301 gtcttaatga gaaacatcag cctatcttct ggaatctccc gtcccatggt caagttgtat
      361 agaaccgtat catggaaaag ggatccctct tgtggcagat aggcaatctt gtcttgaatg
      421 gcttggtagg ataagtctga aagagaggtt ttatcaacgt aaatatcccc tgcttgcagt
      481 tggctattct tggtcaaaat aagagccaag gaagatttgc cagaaccact ttccccaaga
      541 attaaatact tcttgccacg ctcaaacgct acggtcaggt cttcaaagac ctgtttttca
      601 ccgacagcat aactagcctt gaccagttga atactctgga aagctgaagc tagttcctgt
      661 cccttcgttt cttgaccaaa agtagatagc tcaaactctt gctttaattt cttaacggca
      721 tgcctgtcca aaaaggctgc cgcaatgcta ttaatgggat ccaccaactg ctctgaaatg
      781 gtcagaatag ctgtcaagcc accgatggtc aaggcaccag aaaagacttg aaaaccaccg
      841 ataaagataa tcgaaagagt ggtcagataa aagacaaaac cgactaagag ccgcatggtt
      901 actgtcgttc gagtggcctg attgaccttg tcattgactg cttgatagcg atcgttcaga
      961 ccatctaaaa cttgctgtct ttggtggcta tttttcactt gcaaataact cagaaagaca
     1021 tctgaaactc ttgtattata gacctgctga ctgtccgaaa tcgcttcttg attgagcctt
     1081 ctccgctttt taaacaaata gggaatcaca accggcaaga atgagatcag aatcaagaaa
     1141 atagcggtca gaccatccaa gctaagcaag gccaaactgg caatgccaaa ggtacaagca
     1201 ccttgaaaaa tcgtaaagaa agcgtcgtaa taatcttctt tcaccgtctc caaatcatta
     1261 ttgagctggg aaatataggc ccctgccccc tgctcaacaa actgcctaaa aggtaaatca
     1321 aaaattttcc caataaaagt cgcctgcaat tggaaaccta cttgattgag atagctgaca
     1381 ttggcaatct gacgaatgta ttcaaagacc agcatgacaa ggacagttag tcccatcagc
     1441 acaaaatagg acacaaaggc ctgtctatct ccagctgcaa tagcatcaat aatatagcga
     1501 tattggtaca gaaagccaac attgagcagg gctactgcaa aaatacaagt taaggttagc
     1561 caaaaattcc tctttttcat ttctcctcca ctgtttgata gatttctaag caaagcaata
     1621 aaaaaatctg cattaaacac ctttattata ccactccttc ccatatttgt gtccattttc
     1681 acataaagaa aaagaagcta ggcataaagt cca
//
LOCUS       DASFSW010000246         1656 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.249, whole genome shotgun sequence.
ACCESSION   DASFSW010000246 DASFSW010000000
VERSION     DASFSW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.249"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            200..1047
                     /locus_tag="U1099_002177"
                     /pseudo
     CDS             200..1047
                     /locus_tag="U1099_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
     gene            1044..1574
                     /locus_tag="U1099_002178"
     CDS             1044..1574
                     /locus_tag="U1099_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM4868445.1"
                     /translation="MKQNQLRQLTEISLFAALICVVVFFIRIPFGSQFVHLGNALVVV
                     GVLIFGAKKGALAASLGLGIFDLLSGYASVAWITILESLIVCFVLHLFYEKFLQANDS
                     LSNIILVGIVAALTKIILNLLKYTLTGSLVGGLTPSASFLAALGRILGTYGTAITTII
                     AVPILYQVCKRLYKRK"
BASE COUNT          481 a          288 c          363 g          524 t
ORIGIN      
        1 acagaaatta tagagccaca atacattgaa aaggatgaca gtagcaaaga gttgttactg
       61 tttttcttat gtaataattt gagcctggtg ttgtaaggtt tcagctgtct gtccccatac
      121 agttgtggat atgtaagatt gaaaaatgta tgataatcct ttacaatttt gtttaagaaa
      181 aataatttag gaggttgtta tggttcagcg attattgctt gcgaacgact tacctggtgt
      241 tggtaaggtt gcactagcaa ctagtatccc aattgcggct gcttgtcagg tagaaacaat
      301 gcttttacct acagttcttt tatcctcaca cacaggaggt ttcccagatg ttgtcattga
      361 agatatgact gacttgaatc gagcctattt tagacagtgg aagaacttga atttggagtt
      421 ggcaggcatc ttgtctggct actgtagaaa tcctcagcaa ctaagtcaat tggctatgta
      481 tgctaaacaa acagagacac cattgattgt tgattcggtc atgggggatg gagggaagtt
      541 gtatacaggt ttcaccccta ctatgtagaa gccatgaaag agttgataca gtcggcaaag
      601 ctcattctcc ccaatgtaac agaggcagct tttttgacag ggagggatta tttgggagct
      661 tgctacgata gggtggctat tgaggactta ctggaagctt tagcagagca ggtatccgca
      721 gatattgtac tgacagggat ttcctttgat gatagacaaa taggtgtagc ctattttcag
      781 caggaaacaa gggaaataac tacctatatg tctaaaaaat atccagcaaa tttctttgga
      841 acaggtgaca ttctatctac cttgttggct gtagcatcca tccaacaaat caatctccat
      901 caagctatcc ctctagcttt agattttatc gataagagtt tagagcgtac ccttgctttg
      961 gatagagact tgaaatttgg gatttccttt gaaccttttc tagcagaatt acagcaggca
     1021 tttcaaacat taaaggagaa attatgaaac agaatcaatt gcgtcaatta acagaaatta
     1081 gcctatttgc agcccttatt tgtgtggttg tcttctttat tcgcattcct tttgggagtc
     1141 aatttgtcca tttaggcaat gctctggtgg tagtaggagt attgatcttt ggtgccaaaa
     1201 aaggggcgct ggcagcttca ctcggtctag gcatctttga tttactgtcg ggatatgcct
     1261 ccgttgcttg gattaccatt ctagaatcac tcattgtctg ttttgtccta cacttatttt
     1321 atgagaaatt cctacaggca aacgatagtc tatcaaatat tatcttggtt gggatagttg
     1381 cagccctcac aaaaattatc ttgaacctgt tgaagtacac attgacgggt agtttagtcg
     1441 gtggactaac gccatcggca tcatttttag ctgctttggg taggatttta ggaacctatg
     1501 gcactgcaat tacgaccatc attgctgtac ctattcttta tcaggtatgt aaaaggttat
     1561 ataagagaaa gtaatttata ttagtgacat tagttaaatt caatatgcaa aaaaaggtca
     1621 gaaggactac actgtaagtg gcgaaacaaa caggag
//
LOCUS       DASFSW010000247         1632 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.250, whole genome shotgun sequence.
ACCESSION   DASFSW010000247 DASFSW010000000
VERSION     DASFSW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.250"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(131..724)
                     /locus_tag="U1099_002179"
     CDS             complement(131..724)
                     /locus_tag="U1099_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM4868446.1"
                     /translation="MTQIKWRDCLLICFGAILYALVLNMLIIPHEFGEGGITGVTLLL
                     FYTTGIETSLSSFVLNGILIVLAYRFLSKLTVFYTFLAVATMSLTMHYTTFLQIKWLP
                     LIPSALLAGLVTGLSMALVIYGNGSTAGSDIIALLCNKYFKWKISTVILIFDVFVVTP
                     LAFKIGLVKTFWTLVMVVIISKSLDWFLQALKKNNHK"
     gene            complement(802..1185)
                     /locus_tag="U1099_002180"
     CDS             complement(802..1185)
                     /locus_tag="U1099_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEM4868447.1"
                     /translation="MGLFSGLLGNASQMDNDKIEQELEHVLLDNEQVEMAFSLVRDLI
                     VFTEYRLILVDKQGMTGKKVSYKSIPYRSISRFTVETSGHFDLDAELKIWISSAVEPA
                     EILQFKSDKSVIAIQKALASAVLGK"
     gene            complement(1273..>1632)
                     /locus_tag="U1099_002181"
     CDS             complement(1273..>1632)
                     /locus_tag="U1099_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
                     /protein_id="HEM4868448.1"
                     /translation="NLDVEGNQIKTLEIEGKQESVVRLNVADNQLKNLEGINDYKALE
                     DLNASKNDIETLAITEPNKTLKTIDVSENHIPKEELNLNDQKIPSAIAEHFPAVEGGS
                     IENNQPKEVDKEAKVGE"
BASE COUNT          522 a          351 c          242 g          517 t
ORIGIN      
        1 tgatgaactt cagttctatc tgcatctgcg tcgcttagtc tgtttttgat tttcattgag
       61 tattaattca ttcgactata agaaaagaga aaactagtta tacatgcaca actgggtttc
      121 tcttgtattt ttatttatga ttattctttt tcaatgcttg caagaaccag tccaaacttt
      181 tactgataat gacaaccatt accaatgtcc aaaatgtttt tacaagccca attttaaaag
      241 ccaaaggagt taccacaaat acatcaaaaa tgagaataac ggtggaaatc ttccatttga
      301 aatatttatt acacaacaat gcaataatat cacttcctgc cgtggacccg tttccataaa
      361 tgactagagc cattgataaa cctgtcacca atcccgccaa aagagcagaa ggaatcaggg
      421 gcaaccattt tatttgcaga aaagtcgtat aatgcatggt taaggacata gtagcaaccg
      481 ctaagaaagt atagaaaacc gttaacttac tcaaaaagcg ataggctaat acaatcaaaa
      541 tcccattcaa aacaaaactg gacagacttg tttcaatccc tgtcgtataa aacaaaagta
      601 gggtaacacc agtaatccca ccttcgccaa attcatgtgg aatgatgagc atattcaaaa
      661 caagggcata taggattgcc ccaaaacata tcaataagca gtctctccat tttatctgcg
      721 tcatattctc tcctcacaaa cagtccacct tagtatatca aaaaaagcag aaagcggata
      781 gccactttct gtactttttt cctattttcc taagacagct gaagccagtg ctttttgaat
      841 ggcaatgaca ctcttatcgc tcttaaactg aagaatttca gcaggttcaa cagccgatga
      901 aatccatatt ttcagctcag catctaagtc aaaatgacca gaggtttcta ccgtaaaacg
      961 tgaaatcgag cggtaaggaa tcgatttata agataccttt ttcccagtca ttccttgctt
     1021 atcgaccaaa atcaatcgat attctgtaaa aacaatcaga tcacgaacca gactaaatgc
     1081 catctcaacc tgttcattat ccagtagaac atgctccaat tcctgttcaa tcttatcatt
     1141 atccatttgc gaggcattac ctagtaaacc tgaaaatagt cccataatat gactcctttg
     1201 gtatttttct atagtataac acaaaaagct actagttgac tatcttaggt aggtctttac
     1261 caaacatgag aattactcgc ctacctttgc ttccttatca acttctttag gttgattgtt
     1321 ctcgatactg ccaccttcaa ctgctggaaa atgttctgca atcgcagatg ggatcttctg
     1381 atcatttaga tttaattctt ccttcggaat gtgattttca ctcacatcaa ttgttttcaa
     1441 agtcttattt ggctccgtga tggcaagtgt ttcaatatca ttttttgaag cattcaggtc
     1501 ttctaaggct ttgtaatcat tgattccttc taaattcttc aattgattat ctgcaacatt
     1561 taatcgaaca accgattctt gtttaccctc tatttccaaa gtcttaattt gattcccctc
     1621 tacatccaga tt
//
LOCUS       DASFSW010000248         1629 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.251, whole genome shotgun sequence.
ACCESSION   DASFSW010000248 DASFSW010000000
VERSION     DASFSW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.251"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            136..1392
                     /locus_tag="U1099_002182"
     CDS             136..1392
                     /locus_tag="U1099_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEM4868449.1"
                     /translation="MKKKILATIMLSTVVLSNANYVAVISANDVDSQIAAKNQQISEL
                     TAQQAEAQQQVDAIQGQVDAIVSEQAKLTEENTRLEAESQTLAADIERLSADIVSRDG
                     ALKEQARSAQVDGSASSYINTILDSKSIIDAVSRVNAMREIVSANNRMLEQQKADKEA
                     IVEKQKANQEAITTLAANRQKLEDDAQVLQVRQAELEAAKLNLAVQKATAEDEKNSLL
                     VQKAAAEEAARQAAARQAEYQAQQAALAQQQVASVSAPVVSTPVETTVTETFAAPTQT
                     VSQSTPTVSTPTTSTSSGSGSSAAANNARYDASSYPVGECTWGVKSQLSWVGPYWGDA
                     KQWLASARAEGFSTGSTPQVGAIAVWTGGYYGHVAVVTAVQSSTSIQVVESNYMGRRY
                     IGNHRGGYFNPTTTSEGAVYYIYPPY"
     gene            1495..>1629
                     /locus_tag="U1099_002183"
     CDS             1495..>1629
                     /locus_tag="U1099_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HEM4868450.1"
                     /translation="MAFTDLKLFALSSNQKLAEQVSKKIGIPLGKSSVRQFSDGEIQV
                     N"
BASE COUNT          518 a          277 c          383 g          451 t
ORIGIN      
        1 gtgttacaac cattatttga gcgtttattt ggaataacaa acaagacata gaaatgtaat
       61 aattgcgtaa tatacttacg cgattttttt gataaaatga gagagtcaga aagtatagag
      121 gagttatgtt cggatatgaa gaaaaaaatc ttggctacaa ttatgttaag tacagtcgtt
      181 ctatctaatg ctaattatgt agctgtgatt agtgcgaatg atgtagatag tcagattgca
      241 gcaaaaaatc aacagattag tgagttgaca gcacaacaag ctgaagctca acaacaagtt
      301 gatgctattc aaggacaagt tgatgcaatt gttagtgaac aggcgaaatt aacagaagaa
      361 aatactcgtt tggaagcaga atcgcagaca ttggcggcag atattgagcg tttgtcagct
      421 gatattgtgt cacgtgatgg tgctttaaag gagcaggcgc gtagtgctca agttgatggt
      481 tctgcttcaa gttacattaa tacaattttg gattcaaaat caatcattga tgctgtttct
      541 cgtgttaatg caatgcgtga gattgtttca gctaataacc gtatgttaga acaacaaaag
      601 gctgataagg aagcaattgt tgaaaaacaa aaggcaaatc aagaggcaat cactactttg
      661 gcagctaatc gccaaaaatt ggaagatgac gcccaagtat tgcaagtgcg tcaggctgaa
      721 ttggaagctg ctaagttaaa tttggctgtg cagaaagcta ctgctgaaga tgagaaaaat
      781 tcattgttgg tgcaaaaggc agctgcagag gaagcggctc gtcaagcggc agctcgtcaa
      841 gcggagtatc aagcacaaca ggcagctcta gcacagcaac aagtagcctc agtatcagca
      901 ccagtcgtgt caacgccggt agaaactaca gtgactgaaa cgtttgcagc ccctactcag
      961 acagtatcac aatctacacc aacggtaagc acacctacaa cgtctacatc atcaggttca
     1021 ggtagttcag cggcagcaaa taatgcgcgt tatgacgctt catcttaccc agttggtgag
     1081 tgtacttggg gagttaaatc tcaactttca tgggttggtc cttactgggg agatgctaaa
     1141 cagtggttag catcagcacg tgctgaaggt tttagtacag gttctactcc acaagtaggt
     1201 gcgattgctg tttggacagg tggttattat gggcacgtag ctgttgttac ggctgttcaa
     1261 tcttcaacaa gtatccaagt tgttgaatca aactacatgg gtcgtcgtta tattggtaac
     1321 catcgtggtg gttatttcaa tccaacaacg acttctgagg gagcagttta ctacatttac
     1381 cctccatatt aagaaatttt caaatagagc gtgtggattt gcgctctatt tttgtttgaa
     1441 aaatgaagga aaaagggtta aaatagtaaa ggtagaaata tataggaggc tgtcatggcg
     1501 tttactgact taaaattgtt cgctctttcg tcaaatcaga agttagctga acaggtgtcg
     1561 aaaaaaatag gaattcctct gggaaaatca agtgttcgtc aattttcaga tggtgagatt
     1621 caggtaaat
//
LOCUS       DASFSW010000249         1606 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.252, whole genome shotgun sequence.
ACCESSION   DASFSW010000249 DASFSW010000000
VERSION     DASFSW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.252"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            48..257
                     /locus_tag="U1099_002184"
     CDS             48..257
                     /locus_tag="U1099_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEM4868451.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDKALEEYIRKYLPDKL"
     gene            274..546
                     /locus_tag="U1099_002185"
     CDS             274..546
                     /locus_tag="U1099_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM4868452.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            548..706
                     /locus_tag="U1099_002186"
     CDS             548..706
                     /locus_tag="U1099_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM4868453.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGS
                     GKTSLRRR"
     gene            758..862
                     /locus_tag="U1099_002187"
                     /pseudo
     CDS             758..862
                     /locus_tag="U1099_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1282..>1606
                     /locus_tag="U1099_002188"
     CDS             1282..>1606
                     /locus_tag="U1099_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM4868454.1"
                     /translation="MNCLIGGRGTGKSTLIDMLQFVLSQDCDKQSKLEFLCNHANAFV
                     LYVLEDAEYIIEVSLPDVLQENRDNILQYYGQNRENRYGYPYNYNSDSIKEWTRSQYT
                     KVYKVE"
BASE COUNT          616 a          197 c          327 g          466 t
ORIGIN      
        1 ttgatgataa aataagagta agaagaaata gaaagaagtg agtggttgtg ggaaatttag
       61 gcgcacaaaa agcaaaacga aatgatacac caatcagtgc aaaaaaagat attatggggg
      121 ataagacggt tcgtgttcgt gctgacttgc accatattat aaaaatcgaa acagcaaaga
      181 atggcggaaa cgtaaaagaa gttatggata aagccttaga agaatatatt cggaaatatt
      241 tacctgacaa actttaaaag gagtgaaaat gaaatggcag ttacttatga aaaaacattt
      301 gaaatagaga tcattaacga attatcggca agcgtttata atcgagtatt aaactatgtt
      361 ttgaatcatg aattaaataa aaatgactct caattattgg aagtcaattt attaaaccaa
      421 ttaaagcttg caaaacgtgt aaatcttttt gattattctt tagaagaatt acaagccgtt
      481 catgagtatt ggcggtcaat gaatcgctat tcaaaacaag ttttgaataa agagaaagtg
      541 gcttaatatg gcaaatatag tcaattttac tgacaaacaa tttgaaaatc gcttaaatga
      601 taatttagaa gaattggttc aaggaaaaaa agcggttgaa tcgccaaccg cttttttact
      661 tggtgggcaa ccagggtcag ggaaaacgag tttgcgaaga agatgattga gcaggcagga
      721 acaaagtgat aaattcaaat tgtgagggaa aatatagatg aatcaaggaa gaattattgt
      781 gattacaggc tcaccgggga caggaaaaac aacaactgcg tcaattgtcg caaaggaatc
      841 agatatggat aaatcagtgc atatagttat aatattttga aaaaaggttc tcacttgtcg
      901 ggtgcatata agagacaagt ttttacaaaa gaaaatacac gatttattgg tgtaaaaaat
      961 ataaattctg ttgaaggatc gaaaaatcgt ttattgacag attttaattg tgaaccaaat
     1021 tttttattag ataatgattc acatgatatt gattcagtcg gaaagaataa tatgtggata
     1081 aaaggtggaa agatttcttt taaaatgttc caagaagctt tgcttgatta cactgtatca
     1141 gtaagtttat ttgaaccaaa ttttgaacaa aagtcttaca taaaaggttt atacattcaa
     1201 agtcgtggag gcgatagaag ttttcttaca ggtgataagt tagaaaaaga tagagacttc
     1261 tttttaacat tctcaccatc tatgaactgt ttaattggag ggagaggtac agggaaaagt
     1321 acactaatag atatgttaca atttgtactt tctcaggatt gcgacaagca gagtaaatta
     1381 gaattcctat gtaaccatgc aaatgctttt gtactctatg ttttagagga tgctgaatat
     1441 atcattgagg ttagtttgcc agatgtcttg caggaaaata gggacaatat cttacaatat
     1501 tatgggcaaa atcgtgagaa taggtatgga tatccttata actacaattc tgactctatc
     1561 aaagagtgga cacgctctca atatacgaaa gtatacaaag ttgagg
//
LOCUS       DASFSW010000250         1565 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.253, whole genome shotgun sequence.
ACCESSION   DASFSW010000250 DASFSW010000000
VERSION     DASFSW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.253"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>1565)
                     /locus_tag="U1099_002189"
     CDS             complement(<1..>1565)
                     /locus_tag="U1099_002189"
                     /inference="COORDINATES: protein motif:HMM:NF013727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEM4868455.1"
                     /translation="AIVSKLKALTERLSIQALPQPWLPPLPSRLFVEDLRDEAELTWS
                     GVNEELEVILGLLDKPSQQAQEPLSINLSQDGHLLMYGAPATGKTTFLQTTVLDLARH
                     YSPSELHIYLMDFGTSGLVPMGDLPHVADVFLLDQGEKIGKFIRLLEKEINRRKKLLT
                     EHRVGTLSMYRQLTGQVEPRIVIALDSFESMKDESFEAELLKLLTRISREGQAIGLHL
                     VVTASRQANLRATFYSNFKHQLTLKQHDKTEVRSVVGATGLADIEDIKGRALYKGEEV
                     YSLQLALPLRVEHDIELVDQMREEVSRQKAAWTGQVPEAIPMVPEELTREDFLSRASV
                     QKAIASGQIPLGLDLETVEAVSWDLTVGNFVYIYEQERHRNNFLRGFLNTLGQTDYSV
                     KMVSGEEFFTDNGTEVSFTPSSRLLMIDEIYDSVFENIEFDEYDEIVVVIWNELDVLV
                     QEDSQLLDKMLYILTNGPKVGVYSLIVTTPGLSSKIDVVSKHVKLMKEAMVETRLNDQ
                     KILSLNNVKYSEP"
BASE COUNT          458 a          409 c          295 g          403 t
ORIGIN      
        1 acggctctga gtacttaaca ttatttaagc ttagaatttt ttgatcattc aatcgtgttt
       61 ccaccatagc ttctttcatt aacttaacat gcttagaaac aacatcaatc ttagaagata
      121 ggccaggcgt agtaacaatt aaactgtata ctcctacttt aggaccatta gtcaatatat
      181 agagcatctt atctaacaac tgactatcct cttgtactaa aacatctaat tcattccaaa
      241 tgacaacaac tatttcatca tattcatcaa attcgatatt ttcaaaaaca gaatcatata
      301 tttcatcaat cattaataaa cgactcgaag gtgtaaacga aacttccgtc ccattatcag
      361 taaagaactc ttcgccacta accattttta cagaataatc tgtttgtccc agagtattta
      421 aaaaacctcg caaaaagtta tttctatgcc tctcttgttc ataaatatat acaaagttcc
      481 caacggtcaa atcccaactc acagcctcca ccgtttccag atccagacca agcggaatct
      541 gaccagaggc gatggctttc tggacgctgg cacgactgag gaagtcctcg cgagtcagct
      601 cttctggaac cattgggata gcttctggaa cctgacccgt ccaagcagcc ttctgacgac
      661 tcacttcctc acgcatctga tccaccagct ctatatcgtg ttccacacga aggggaagtg
      721 ccaactggag gctataaacc tcctcacctt tatagagggc ccgtccctta atatcctcta
      781 tatccgcaag accagtagcc ccgaccacac tacggacttc tgtcttatca tgttgcttca
      841 aggtcaactg gtgcttgaag ttagagtaga aggtcgcacg aaggttagcc tgacgactag
      901 ccgtcaccac aaggtgaaga ccaatggcct gaccctcacg agaaatacgg gtcaagagtt
      961 tgagtaactc tgcctcaaaa ctctcatcct tcatagactc gaagctatcc aaggcgatga
     1021 caatacgtgg ctccacctgt cctgtcaact gacggtacat ggacaaggta cccacacgat
     1081 gttccgtaag cagcttcttg cgacggttga tttccttctc caaaagacgg ataaacttac
     1141 cgatcttctc tccctgatcc aagaggaaga catccgccac atgtggtaaa tcccccattg
     1201 gaaccagacc actcgtccca aagtccatca gataaatatg gagttcagat gggctatagt
     1261 gacgagctaa atccaagacc gtagtctgta agaaggtagt cttaccggtc gctggagccc
     1321 catacataag caaatgacca tcctgagata ggttgataga cagcggttct tgggcctgct
     1381 ggctcggttt atccaagaga cctagaataa cctctaactc ctcattgact ccagaccaag
     1441 ttaactcagc ctcatctcgt aaatcttcca caaacaagcg cgaaggcaag ggaggcaacc
     1501 atggttgcgg caaggcctgg atggacaggc gctctgtcaa ggctttcagc ttacttacga
     1561 tggct
//
LOCUS       DASFSW010000251         1515 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.254, whole genome shotgun sequence.
ACCESSION   DASFSW010000251 DASFSW010000000
VERSION     DASFSW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.254"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(15..566)
                     /locus_tag="U1099_002190"
     CDS             complement(15..566)
                     /locus_tag="U1099_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM4868456.1"
                     /translation="MTKALISIDYTIDFVADEGKLTAGKSAQAISERIAQVTQEAFEN
                     GDYIFFAIDGHEEGDEFHPEAQLFPSHNIIGTQGRDLYGPLADFYQKHKGHARVRWMD
                     KRHYSAFSGTDLDVRLRERGVDTVVLTGVLSDICVLHTAIDAYNKGYRIEVVSSAIAA
                     LTEENHQFALNHLRHVLGATIID"
     gene            complement(568..1356)
                     /gene="codY"
                     /locus_tag="U1099_002191"
     CDS             complement(568..1356)
                     /gene="codY"
                     /locus_tag="U1099_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEM4868457.1"
                     /translation="MTTLLEKTRNVTSILKRSEEQLAEELPYNAIAEHLSAIIDCNSC
                     IINSEGEVLGCHMSYETNNDRVEEFFQNKQFPEGYVKAVAQVYDTQVNLPVESELTAI
                     PVESRSTYPNGLTTIAPIHVTGIRFGSLIIWRNDEQFHDDDLILVEIAATVVGIQLLN
                     FQREEDEKNIRRRAAVNMAVNTLSYSEMKAVAAILGELAGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLIPAIFDEIKKRDY"
BASE COUNT          483 a          373 c          236 g          423 t
ORIGIN      
        1 tccccactat actattagtc aataattgtc gcaccgagta catgacgcaa atggttaaga
       61 gcaaactgat gattctcctc cgtcaatgca gcaatggctg atgagaccac ctcaatacga
      121 taccccttat tataagcatc aatagctgta tgaagaacac aaatatcaga caaaacaccc
      181 gtcaagacaa ccgtatctac accacgctct cttagtcgaa catccaaatc cgtcccagaa
      241 aaagcagaat aatgacgttt atccatccaa cgaacacgcg catgcccttt atgtttttga
      301 taaaaatctg ctaaaggacc atacaaatca cgtccttgcg tcccaatgat attatgactt
      361 gggaaaagct gagcttcagg atgaaattca tccccctcct catgaccatc aatcgcaaag
      421 aaaatatagt ctccattttc gaaagcttct tgcgtaacct gagcaattcg ttcagaaata
      481 gcctgagctg attttccagc agtcaacttt ccttcatctg ccacaaaatc tattgtataa
      541 tcaatcgaaa tcaatgcttt tgtcatttta gtagtcacgt ttcttaattt catcaaaaat
      601 agctgggatg agaactttca aataagtccc cttcattccc aaagaacgac tttcaataat
      661 ccctgcactc tccaacttac gcagtgcatt cacaatcacc gagcgtgtaa taccgatacg
      721 atctgcaatc acagaagcag tcaattgccc ctcattgcca gccaattcac ccaaaatagc
      781 tgcaactgct ttcatttctg agtaagatag tgtatttacc gccatattaa ctgccgcacg
      841 acgacggata ttcttctcgt cttcttcccg ttggaaatta agtaactgaa taccaactac
      901 tgttgccgca atctcaacca aaatcaaatc atcatcgtga aactgctcat cattccgcca
      961 aataataagc gaaccaaaac gaatccccgt tacgtggata ggcgctatcg ttgtcagccc
     1021 gtttggataa gtcgagcgtg attcgacagg gatggcagtc aactcgctct cgacaggcaa
     1081 attaacctgc gtatcgtaaa cctgcgcaac tgcttttaca tatccttctg ggaattgttt
     1141 gttttggaaa aattcttcca cacgatcatt gtttgtctca taactcatgt ggcatcccaa
     1201 aacttcacct tcgctattaa taatacaaga attgcaatca ataatagctg atagatgttc
     1261 agcaatagca ttgtaaggca attcttctgc caattgctct tcggaacgct tcaaaataga
     1321 agtaacattc cgtgtcttct ctaataatgt tgtcatattt tcctctttac tctgtaattg
     1381 ctttcagtat agcaaaaaat acataaaatt tcaacaatta tcagaatatt taccgttgtt
     1441 agaaaaacac aaacaatttc atccaagcaa aaagaaatag gctgggcaaa aagcccagca
     1501 ccgcttctca aagtt
//
LOCUS       DASFSW010000252         1495 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.255, whole genome shotgun sequence.
ACCESSION   DASFSW010000252 DASFSW010000000
VERSION     DASFSW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.255"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            86..832
                     /locus_tag="U1099_002192"
     CDS             86..832
                     /locus_tag="U1099_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM4868458.1"
                     /translation="MATYETFAAVYDEIMDDSLYDKWTDFSLRNLPQDTKTILELACG
                     TGIQAVRFAQKGFEVTGLDLSYEMLELAQKKAEVAGVMMELAQADMMALEGVGDFDAV
                     TCYSDSLCYMADQEAVLRVFEGVHSVLNQGGTFLFDVHSIYQMEEVFAGYSYHENYED
                     FAFVWDTYEGEYAHSIVHELTFFVKDEDSQEERFIRRDEVHEERTYPIETYVELLKEA
                     GFASVEVFADFENQAPTETSQRWFFKCKKK"
     gene            845..>1495
                     /locus_tag="U1099_002193"
     CDS             845..>1495
                     /locus_tag="U1099_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868459.1"
                     /translation="MNVAIIILVAIFVILLLLLLVYVALFQIWLKSSRQIHEYDEDII
                     RDYLCKLAESFEYSSTQIPYGRARWFILGSKDFVCTVNDVENLEIFGYSPIRSKIDEE
                     FLEYGLLLTQDGFYFREQIENSNNDADVKYKISKKFFPLKGLWKITCKGKKILLFYPN
                     KIERLNFQFDDAQQKNIVANLNSIIKTGYTADLHENYLEKVIDRLQKNDFHTENFQN"
BASE COUNT          456 a          213 c          350 g          476 t
ORIGIN      
        1 attagaagtg atgaacttct tgaacacagt gtgagttaat gaagcagtcg attggctgct
       61 ttttatgaaa gggaaaatgg gaaatatggc aacttacgaa acctttgcgg cggtctatga
      121 tgaaatcatg gatgacagct tgtatgataa atggacagat tttagcttgc ggaacttgcc
      181 gcaagacacc aagacaattt tagaattggc ctgtgggaca gggattcagg ctgtgcgttt
      241 tgctcaaaag ggctttgaag tgacagggct tgacctatcc tatgagatgt tggaattggc
      301 tcagaaaaaa gcagaagttg ctggtgtcat gatggaattg gcccaggcag atatgatggc
      361 tctggaaggt gttggagatt tcgatgcggt gacctgttac tcggacagcc tttgctacat
      421 ggcggaccaa gaagcagttt tgcgggtctt tgaaggagtt cactctgttc tcaatcaagg
      481 tggtactttc ctttttgacg ttcactccat ctatcaaatg gaggaagtct ttgcaggcta
      541 tagctaccat gaaaactacg aggattttgc ctttgtctgg gacacctatg agggggagta
      601 tgcacattcg attgttcacg aattgacctt ctttgtcaaa gatgaagaca gtcaagagga
      661 gcggtttatt cgtcgggatg aagttcatga agagcggacc tatccgattg aaacctatgt
      721 ggaattattg aaagaagctg gctttgcctc tgtggaagtc tttgcggact ttgaaaatca
      781 agctccgaca gaaactagcc agcggtggtt tttcaagtgt aagaagaaat aggtggtatt
      841 ggatatgaat gtagctatca ttatcttggt tgcaatattt gttattcttt tattgttatt
      901 attagtttat gtagctttgt ttcaaatatg gttaaaatca agtcgtcaaa tacatgaata
      961 cgatgaagat attattcgtg attatttatg caaattagcc gagtcttttg aatattctag
     1021 tacgcagatt ccctacggtc gggctagatg gtttatattg gggagcaaag attttgtttg
     1081 tacagttaat gacgtagaga atttggagat ttttggctat agtcctatta ggagcaaaat
     1141 tgacgaggag tttttggaat atggtttgtt gttaacccag gatggatttt attttagaga
     1201 acaaatcgaa aatagcaata atgatgctga tgttaagtat aaaattagca aaaagttttt
     1261 ccctttaaaa ggtttatgga agattacttg taagggtaaa aaaattctac ttttttatcc
     1321 taataagatt gagagactaa attttcaatt tgatgacgct caacaaaaaa atatagttgc
     1381 aaatttaaac tcaattatca aaacaggtta tacggcagat ttacatgaaa actatttaga
     1441 aaaagttatt gatcgtttgc aaaaaaatga ttttcataca gagaattttc aaaat
//
LOCUS       DASFSW010000253         1493 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.256, whole genome shotgun sequence.
ACCESSION   DASFSW010000253 DASFSW010000000
VERSION     DASFSW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.256"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..1257)
                     /locus_tag="U1099_002194"
     CDS             complement(<1..1257)
                     /locus_tag="U1099_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEM4868460.1"
                     /translation="MKSRIVSPKTIFIWNLLGSISSAAISIFLLLLVTRLLTELEADI
                     FSFAYAVANLFVIIASFQVRDYQATDVSKKFSFSQYLATRLITITIMLLLALSYIFLS
                     KYEFQKSACIFLICLYRGSDALSDVFQGLFQQNARLDIAGKSLFLRNSIVILTFGFGL
                     FITNNLLLSLIYLVISSYLFVFFFDVTNLFQFTRIIKEEINLKAIKNILLECLPLFIN
                     AFLLVTIYNQPKYALNTFFERGVIGTGVQRDFNILFMPVFSMNILLILFRPMITQLAI
                     YRRAGDYNQFKQYQKRIVKMVVGLAVLVLVGGIVLGIPALNILYGTNLNKYWLSFIIT
                     MLGGIASTFATICDNMLTVLRKQKYLVISFAISCLLSILISNPLVEYYGILGAAIAFV
                     SSMWTWFLISFVIYLKLQKHLNWEVQE"
BASE COUNT          563 a          276 c          184 g          470 t
ORIGIN      
        1 ttcttgtact tcccaattca aatgtttttg caattttaga tatattacga aagaaattaa
       61 aaaccaagtc cacatcgaac taacaaaagc aatagctgcc cccaatattc cataatattc
      121 taccagtgga ttagaaatca atatggataa taaacaagaa attgcaaatg aaattacgag
      181 atacttttgc ttcctcaaaa cagtaagcat gttatcacaa atggttgcaa aggtactcgc
      241 aattccccca agcattgtta taataaagct gagccagtat ttattaagat tcgttccgta
      301 gagaatattt aatgctggaa tccccaatac tattccccca accaaaacta acacagccaa
      361 tccgacaacc attttaacga tacgcttctg atattgttta aattgattat agtccccagc
      421 tcttctatat atagccaact gagttatcat gggtctaaac aaaatcaaca aaatattcat
      481 tgaaaaaaca ggcataaata aaatattaaa atctctttga acacctgttc ctataacacc
      541 tctctcaaaa aaagtattga gagcatattt aggttgatta tagatagtaa caagtaaaaa
      601 tgcattaata aataacggca aacattctaa taatatattt ttaatggctt tcaaattaat
      661 ttcttctttg ataatcctcg taaattgaaa caaattcgta acatcaaaaa agaaaacaaa
      721 taaatatgag ctaattacta gatagattaa tgagagtagc aggttattgg ttataaacaa
      781 accaaatcca aatgtcaaaa tcacaataga gtttctcaaa aaaagtgatt tacctgcaat
      841 atctagccga gcattttgtt ggaacaatcc ttgaaaaaca tccgatagag catcacttcc
      901 acgatacaaa catattagga aaatacaagc agatttttga aattcatatt tagacagaaa
      961 aatatagctt aaggcaagta atagcataat agtgattgtt ataagccttg ttgcaagata
     1021 ctggctaaac gaaaactttt tcgaaacatc agtagcttga taatctctta cctgaaaact
     1081 agcaatgatt acaaaaagat ttgctactgc ataagcaaaa ctaaaaatat cagcctctaa
     1141 ttccgtcaac aaacgtgtta ctagtaacaa taaaaatatt gatattgctg ctgaagaaat
     1201 acttcctaat aaattccaaa taaatattgt ttttggtgat actattctgc tcttcacact
     1261 atcttcccct tgtttgtcat ccgttatccc taatttttta atagagattt ctttatcatc
     1321 ttaaacacgc ttacaattcc aactgatatt atctttagtg tccttctcag tccaatttgt
     1381 aaaaattcct tttcttcaat aattaaacgc aattctcttt cgctagaaaa actaatcttt
     1441 tccaatattg gactcacatt ttcagtattc tcgaccactg cataatcgtt tga
//
LOCUS       DASFSW010000254         1477 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.257, whole genome shotgun sequence.
ACCESSION   DASFSW010000254 DASFSW010000000
VERSION     DASFSW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.257"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            33..773
                     /locus_tag="U1099_002195"
     CDS             33..773
                     /locus_tag="U1099_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868461.1"
                     /translation="MDKDSILKVQGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEG"
     gene            776..>1477
                     /locus_tag="U1099_002196"
     CDS             776..>1477
                     /locus_tag="U1099_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEM4868462.1"
                     /translation="MLRLVYYQFIHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVVSSLINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQMSAVGCIITVV
                     LISLLAFLSGCFYTFKKIRKRKSSKIRHVLSILKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQMIFNIVILDI"
BASE COUNT          470 a          195 c          299 g          513 t
ORIGIN      
        1 cttttcaaaa ttaagtgatt gggggcaagc ctatggataa agattctata ttaaaagtgc
       61 aagggctgta tcatcaattt ttactaggaa acaataaaac gttgcaagtg ctgaaaaatg
      121 tttctctttc tgcttcgaga ggagaattta taagtattct aggaattagt ggttctggaa
      181 agtcaacttt attaaaatgt atttctagtt tgcttgaacc aacaagtggg gaagtaattt
      241 taaatggaat caacccctat aaaatcagaa atgcaaaatt gtcaagtata agacgtaacg
      301 aagtatcttt tatatttcaa gcatacaatt taataccttc cctgccagtt atagaaaata
      361 tagcgcttcc tttgcgatta tcacaaaaaa aattaactat taaaaatgta gaaaacttac
      421 tcaaaagaat gaagtttaat gctggcttaa acgattttgt tggaactctg tctggtggag
      481 aacaacagaa ggttgctata gctagagcgg tgattgctga tagtgatata atatttgctg
      541 atgagccaac tggggcttta gacagcgttt ctcgtgaagt aatttttgag ttattgagag
      601 agttagtagg ggcgggtaag tgtgtaatta tggtaacgca tgatatagaa ttggcctcga
      661 aaactgatcg tgcattaata ttgaaagatg gaaaaatttt caaagaactt catagaccta
      721 gcggggaaga gttgtataaa atcttagagg tacaatcaac tacggaggga tagagatgct
      781 cagattagtt tactatcaat ttatacataa taagaaacaa tggttaggcg tttcgccagt
      841 aatttttgtt tcgagtttag ttatgggatt ggctgttaat ggggtaatta atgttgagaa
      901 taattcgcag gtatttgtcg gacttccaga tcctaaacca atttttatgt ttccgatagt
      961 attcggagga gtaacattat tctttgtggt ctctagccta ataaatatgt tggttgaaat
     1021 ttttagagat gattatgaac ttctggaggt gttgggagca agtcgccttc aactatcatt
     1081 tcttgtgggg ggacagattt ttatcatttc ctcgattatt agttttattg catatctatg
     1141 ctctatattt gttacgtcaa attattatta ttttttacaa tactttttcg gggaaaatat
     1201 attacctgat atccaatttc aaatgagtgc tgttggatgt atcattacag tagtcttgat
     1261 ttcattatta gcctttctaa gtggttgctt ttatacgttc aaaaagatta gaaagaggaa
     1321 gagttctaaa ataaggcatg ttctttcaat attaaaaaga atactgctat tggctggttt
     1381 tagtgttatc tggttgcttt ctttgcaaca aattttccaa gatagcacaa ttttagcgaa
     1441 ggcccagatg atattcaata ttgtaatctt agatatt
//
LOCUS       DASFSW010000255         1464 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.258, whole genome shotgun sequence.
ACCESSION   DASFSW010000255 DASFSW010000000
VERSION     DASFSW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.258"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(179..>1464)
                     /gene="purB"
                     /locus_tag="U1099_002197"
     CDS             complement(179..>1464)
                     /gene="purB"
                     /locus_tag="U1099_002197"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEM4868463.1"
                     /translation="RYTRPEMGNIWSEENKYKAWLEVEILADEAWAELGEIPKEDVAL
                     IREKATFDIDRILEIEEETRHDVVAFTRAVSESLGEERKWVHYGLTSTDVVDTAYGYL
                     YKQANDIIRRDLENFTNIIADKAREHKYTIMMGRTHGVHAEPTTFGLKLATWYSEMKR
                     NMERFDVAAKGVEAGKISGAVGNFANIPPFVEEYVCGKLGIRPQEISTQVLPRDLHAE
                     YFSALALIATSIERMATEIRGLQKSEQREVEEYFAKGQKGSSAMPHKRNPIGSENMTG
                     LARVVRGHLVTAFENVALWHERDISHSSAERIITPDTTILINYMLNRFGNIVKNLTVF
                     PENMKRNMESTFGLIYSQRVMLSLIEKGMTREEAYDLVQPKTAQSWDNQVDFKPLLEA
                     DERVTAKLSQEEIDELFNPDYYAKRVDDIFERLGL"
BASE COUNT          374 a          370 c          312 g          408 t
ORIGIN      
        1 ttttgaataa gttgtgagct ggcttcccct acacttatct attcgtaaac ttttgcaaaa
       61 aataatttat ttttacttac gaatgattgt atagcggaga aagcttgttt tccccttcaa
      121 ctatttattc gcaagtaaat aaaaaaattc tctggaaaat ccaaagaatt ttgatttttt
      181 ataatccaag tcgttcaaag atgtcatcaa cccgcttagc atagtagtct gggttgaaga
      241 gttcatcgat ttcttcctgg ctaagtttgg cagtcacacg ctcatccgct tcgagaaggg
      301 gcttgaagtc cacttgatta tcccatgact gcgcagtttt tggttgcacc aaatcatagg
      361 cttcctcacg ggtcatgcct ttttcaatca aactgagcat gacacgctgg ctgtaaatca
      421 agccaaaggt tgactccata ttgcgtttca tattttcagg gaagaccgtc aagttcttaa
      481 cgatattgcc aaaacggttg agcatataat tgatgaggat agtcgtatcc ggcgtgataa
      541 tccgctccgc tgacgagtgg gaaatatcac gttcgtgcca gagagccaca ttctcaaaag
      601 ccgtcaccaa gtgaccacgc accacacgag ccagaccggt catattttca gagccgatcg
      661 ggttgcgttt atggggcata gcagagctgc ctttttggcc tttggcgaaa tactcttcga
      721 cttcacgttg ttcagatttt tgtagtccac ggatttctgt cgccatacgc tcaatagacg
      781 ttgcaatcaa ggctagggct gagaaatatt ctgcgtggag gtcacgaggt aggacctggg
      841 tagaaatttc ctgcggacga atgcccaatt taccacaaac atactcttcc acaaatggag
      901 gaatgttggc aaagtttcca accgctcctg aaattttacc agcctcaacg cccttagccg
      961 ccacatcaaa acgctccata ttgcgtttca tttcgctata ccaagtcgcc aacttcagac
     1021 cgaaggtcgt tggctccgca tggacaccgt gggtccgccc catcataatg gtgtacttgt
     1081 gctcacgcgc cttgtcggcg ataatgttgg taaagttttc aaggtcacga cggatgatgt
     1141 cgttggcctg cttatagagg tagccgtaag ccgtatccac cacgtcggtc gaagtcagac
     1201 cgtagtggac ccacttgcgt tcctcaccaa gactttccga aaccgcacgc gtaaaagcca
     1261 ccacatcgtg acgggtttcc tcttcaatct ctaaaatgcg gtcgatgtca aaagtcgcct
     1321 tctcacgaat caaggccaca tcttctttgg gaatctcacc caactcagcc caagcctcat
     1381 cagcgaggat ttccacctcc aaccaagcct tgtacttgtt ctcttcactc caaatattgc
     1441 ccatttcagg gcgtgtgtag cgtt
//
LOCUS       DASFSW010000256         1439 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.259, whole genome shotgun sequence.
ACCESSION   DASFSW010000256 DASFSW010000000
VERSION     DASFSW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.259"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            73..552
                     /gene="rlmH"
                     /locus_tag="U1099_002198"
     CDS             73..552
                     /gene="rlmH"
                     /locus_tag="U1099_002198"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEM4868464.1"
                     /translation="MKIKLITVGKLKEKYLKEGIAEYSKRLGRFTKLDMIELPDEKTP
                     DKASQAENEQILKKEADRIMSKIGERDFVIALAIEGKQFPSEEFSQKLSDIAVNGYSD
                     ITFIIGGSLGLDSCIKKRANLLMSFGQLTLPHQLMKLVLIEQIYRAFMIQQGSPYHK"
     gene            577..1260
                     /locus_tag="U1099_002199"
     CDS             577..1260
                     /locus_tag="U1099_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEM4868465.1"
                     /translation="MNQKEYRVFEGLRIACSLTFISGYLNAFTFVTQGGRFAGVQSGN
                     VISLAYFLAKGDLAQVVNFSIPILFFVFGQFFTYLARRYFEKQTWSWHFGSSVMMLVL
                     ILLTIILSPIMPASFTIASLAFVASIQVETFRRLRGAPYANVMMTGNVKNAAYLWFKG
                     VIEKDSELRKTGRNILLTIIGFMLGVIISTHLSFQFEEYALIGLILPVLYINYELWQE
                     KRPTRGRSK"
     gene            complement(1258..1331)
                     /locus_tag="U1099_002200"
     tRNA            complement(1258..1331)
                     /locus_tag="U1099_002200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1295..1297),aa:Arg,seq:tct)
BASE COUNT          468 a          220 c          272 g          479 t
ORIGIN      
        1 aaactgtagc catatcctaa ttttctaaaa atattttctg tcgtatgctt gtggatatgt
       61 tacaattatt ttatgaaaat aaaattgatt accgttggaa aattgaaaga aaagtacctc
      121 aaagaaggta ttgcagaata tagtaaacga ttgggacgat ttactaagtt ggatatgatt
      181 gagcttcctg atgaaaaaac accagataaa gccagtcagg cagagaatga acaaatatta
      241 aaaaaagaag ccgatagaat tatgtctaaa attggagagc gagattttgt cattgcctta
      301 gcgatagaag ggaaacaatt tccatcggaa gaatttagtc aaaagctatc tgatattgca
      361 gtaaatgggt attcagatat aacttttatc atcggtggta gtttgggtct cgattcttgt
      421 attaaaaaaa gagctaattt gttgatgagt tttggacagt tgacacttcc acatcaatta
      481 atgaaattag ttctcatcga gcagatttat cgtgcattta tgattcagca gggaagccca
      541 tatcataagt agtttcacgt gaaacaagga gtgaaaatga atcaaaaaga gtatcgtgtt
      601 tttgagggat tgagaattgc ttgttcatta acgtttatca gtggttattt aaatgccttt
      661 acttttgtga ctcagggtgg tcgctttgct ggcgtacaat ctggaaatgt tatttcccta
      721 gcttattttt tagctaaagg tgaccttgcg caggtagtta atttttccat tcccatttta
      781 ttttttgtat tcggacaatt ttttacctac ttagcaagaa ggtattttga aaaacaaaca
      841 tggtcttggc actttggtag tagtgtaatg atgttagttc ttattttact aactatcatt
      901 ctctcaccta taatgcctgc gtcttttaca attgctagtc tagccttcgt agcctctatt
      961 caagtagaaa catttagaag gttacgaggt gctccgtatg ccaatgtgat gatgacaggg
     1021 aatgtcaaaa atgctgctta tctctggttt aaaggagtta ttgaaaaaga ttcagaactt
     1081 agaaaaacag gtagaaatat cttattaact attatagggt ttatgctagg tgtcatcata
     1141 tctactcacc tatccttcca atttgaagaa tatgccctta tcggtctgat tttgccagtt
     1201 ttatatatta attatgaatt atggcaagaa aaaagaccta ctcgaggtag gtctaaatga
     1261 ttccagcagg attcgaacct gcgaccgttc gcttagaagg cgaatgctct atccagctga
     1321 gctatggaac ctcaactatt ttattttaca ttaaacaaag cactctgtca atacaataaa
     1381 aaatcaaaaa atgtattgac aaacaaagtc acatgtatta tactagttat tgttctaaa
//
LOCUS       DASFSW010000257         1437 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.260, whole genome shotgun sequence.
ACCESSION   DASFSW010000257 DASFSW010000000
VERSION     DASFSW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.260"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1372
                     /locus_tag="U1099_002201"
     CDS             <1..1372
                     /locus_tag="U1099_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEM4868466.1"
                     /translation="FLTDVNFLLTLEFLIAYFILFVAMIRYKSSLKWINIVLLFFTFL
                     ELGLHSHYQVQGISDEWHFPSRSNYEEKLTDIDSIVKSTKTTTDSFYRIERLLPQTGN
                     DSMKFNYNGISQFSSIRNRASSSALDKLGFRSDGTNLNLRYQNNTIIADSLFGVKYNL
                     ATTDPNKFGFTLNQSQSTINLYENSFNLGLALLTEGIYKDVNFTNLTLDNQTNFLNQL
                     TGLSQKYYHTLSDVISQNTVELSNRMTVNKVDNEDAAKATFLVNIPANSQVYLNLPNL
                     TFSNENQKKVVITVNNQSSEFTLDNAFSFFNVGSFTTDVQVQVDVYFPENNQVSFDKP
                     QFYRLDLLAFQQAISILQEKQVVTKTDGNKVTVDFVTDKEASLLLTLPYDKGWNATID
                     GKPIKIQKVQEGFMKVDVSPGQTKLVLTFVPNGFYLGLLISFGAVFVFFSYQFIGYYY
                     SKNREY"
BASE COUNT          478 a          261 c          215 g          483 t
ORIGIN      
        1 gtttctaacg gatgtaaatt tcctactcac actagaattt ctaatagcct acttcattct
       61 ttttgtagca atgattcgct ataaatcatc cttaaaatgg attaatattg ttttattgtt
      121 cttcactttc ttagaacttg gattacatag tcattatcaa gttcagggga tttctgatga
      181 atggcatttt cctagccgat caaactatga agaaaaattg acagatattg acagtattgt
      241 aaagtctaca aagactacaa ctgattcatt ttatcgtata gaacgtctat taccacaaac
      301 tggcaatgat agcatgaaat tcaattacaa cggtatttct caattctcct ccattcgaaa
      361 tcgtgcttcc agctcagcac tagataagct cggtttccgt tcagacggta ccaatttgaa
      421 tcttcgttat cagaataata caattattgc tgatagccta ttcggagtca aatataattt
      481 agctactacg gacccaaata agtttggttt cacactgaat cagagtcagt ctactattaa
      541 tctttacgaa aatagtttta atctagggtt agccctattg actgagggga tttacaaaga
      601 tgtcaatttt acaaatctga ctctcgataa tcaaacaaat ttcctcaatc aactaacagg
      661 gctatctcaa aaatactacc acactttatc agatgttatt tcacaaaata cagttgaact
      721 gagcaatcga atgactgtca acaaagttga caatgaggat gctgcaaaag caactttctt
      781 agtaaatata cctgcaaata gccaagtata cttaaatttg ccaaatttaa cattctcaaa
      841 tgaaaatcaa aaaaaggttg tcattactgt caataatcag tcaagtgagt ttacattaga
      901 taatgctttc tctttcttca atgtggggag ctttactaca gatgtacaag ttcaagtcga
      961 tgtatatttt cctgaaaata atcaagtttc ttttgataaa ccacaatttt atcgattgga
     1021 cttattagct ttccaacagg ctatttccat tctccaagaa aaacaagtag taacaaaaac
     1081 tgatggtaat aaagtaaccg ttgattttgt aaccgataaa gaagcctcac tcctccttac
     1141 tttaccctat gataaaggat ggaatgcaac cattgatggt aaacctatca aaatccaaaa
     1201 agtgcaagaa ggttttatga aagtggatgt aagtccaggt caaactaaac tggttttaac
     1261 ctttgtacca aatggtttct atctaggttt actgatttct tttggtgcag tttttgtatt
     1321 tttctcctat caattcattg gatactatta ttctaagaac cgagaatact aaactttctc
     1381 ggttttttct tgtaaaaagc aaaaaacctt gatttctcaa ggtttcctat gctttat
//
LOCUS       DASFSW010000258         1410 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.261, whole genome shotgun sequence.
ACCESSION   DASFSW010000258 DASFSW010000000
VERSION     DASFSW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.261"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            16..111
                     /locus_tag="U1099_002202"
     CDS             16..111
                     /locus_tag="U1099_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEM4868467.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKNSDYADK"
     gene            236..973
                     /gene="erm(B)"
                     /locus_tag="U1099_002203"
     CDS             236..973
                     /gene="erm(B)"
                     /locus_tag="U1099_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM4868468.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1119..1403
                     /locus_tag="U1099_002204"
     CDS             1119..1403
                     /locus_tag="U1099_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEM4868469.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
BASE COUNT          531 a          232 c          235 g          412 t
ORIGIN      
        1 aaggagggat tcgtcatgtt ggtattccaa atgcgttatc aaatgcgtta tgtagataaa
       61 acatctactg ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag
      121 attaattcct accagtgact aatcttatga ctttttaaac agataactaa aattacaaac
      181 aaatcgttta acttctgtat ttgtttatag atgtaatcac ttcaggagtg attaaatgaa
      241 caaaaatata aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat
      301 aaaacaattg aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca
      361 tttaacgacg aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca
      421 tctattcaac ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca
      481 agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc
      541 ttaccattta agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat
      601 ctatctgatt gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg
      661 gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt
      721 tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt
      781 tccagataaa tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata
      841 tcgtcaactg tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa
      901 tttaagtacc gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa
      961 cgggaggaaa taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag
     1021 ggaatgtaga taaattatta ggtatactac tgacagcttc caaggagcta aagaggtccc
     1081 tagcgcctac ggggaatttg tatcgataag gggtacaaat tcccactaag cgctcgggac
     1141 cccttgtagg aaaatgtcct aagtgtggca acaatattgt attaaaaaaa tcgttttatg
     1201 gttgttcaaa ttatcctgaa tgtaccttca ctttagctga acattttaga aagaaaaaac
     1261 tcaccaaaac aaatgtaaaa gaattactag agggaaaaga aaccctggta aaaggaatca
     1321 aaacgaaaga tagaaagtcc tacaatgccg ttgtaaaaat cggagaaaag ggatatattg
     1381 attttatctc tttttcaaaa taagcataaa
//
LOCUS       DASFSW010000259         1384 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.262, whole genome shotgun sequence.
ACCESSION   DASFSW010000259 DASFSW010000000
VERSION     DASFSW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.262"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            379..723
                     /locus_tag="U1099_002205"
     CDS             379..723
                     /locus_tag="U1099_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868470.1"
                     /translation="MKRIVTKRSYKVGLCAMALALLMSTAGGVTAGAQDYHVESSGEL
                     KPMSEGVFMKLESSSNFIENEGIVVKSISNQNTNPNNLNESWYWKFWKKVKLFFKRFR
                     EGFGIQRLFGFK"
     gene            833..1177
                     /locus_tag="U1099_002206"
     CDS             833..1177
                     /locus_tag="U1099_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868471.1"
                     /translation="MKQANSKLAALTLITTLAVTSVIGTTVHAEEKVTADQTVAGKHK
                     PITDEQLQKAIGDRNPNNIKIQAKFENTNFKNQGITFIGKAEIPSKSSENSSWFWNLW
                     TRFTTFVKGIFS"
BASE COUNT          465 a          198 c          232 g          489 t
ORIGIN      
        1 tttgcaaatc cttcatacct tcattaatct ttcttaaatt tttaattttc tataatatta
       61 ccctcttaaa aatctttttc atctatacat tcataacttt aataatataa ctcaaaatag
      121 tatgccctct gtgaaaatag agggctttaa attttttagg gatattcttt ttattacttc
      181 gcttattcga gacaaggttc tcttgaacgg agcgtatcct atttcctgat ttttatatta
      241 atatttttat tttttggaat ttaatggttt tttatctaat ctttctctaa agttggaatt
      301 tacgttattt ttttgtgcta tactggcact acattgtcta gaaaagggcg aatattgtat
      361 aaaggagaca tttcaaaaat gaaacgtatt gtaacaaaac gtagttataa agttggtctg
      421 tgtgctatgg ctcttgctct gttgatgagc actgcaggtg gcgtaactgc aggtgcacaa
      481 gactatcatg tcgaatcatc aggcgaatta aaaccaatga gcgaaggagt gtttatgaaa
      541 ttggaatctt catctaattt cattgaaaat gaaggaatcg tggtgaaatc tattagtaat
      601 caaaatacta atccaaataa tttgaacgag tcttggtatt ggaaattttg gaagaaagtc
      661 aaactgtttt tcaaaagatt tcgtgaaggt tttggtatac aaagattatt tggatttaaa
      721 tagtcaataa ttaaagtgtt attcaaatgt tcataatctt gtaatattta gcgcaattaa
      781 tttataaacc ttcatattaa aaaaactaat actttgtaaa aggagacatt tcatgaaaca
      841 agctaattca aaactagcag ctttaacact cattacaact cttgcagtta cctctgttat
      901 tgggacaacg gtgcatgctg aggagaaagt aactgctgat cagactgtag caggaaaaca
      961 caaaccgatt acggatgagc agcttcaaaa agcgattgga gatagaaatc caaataatat
     1021 taagatacaa gctaaatttg aaaataccaa tttcaaaaat caaggaataa cttttattgg
     1081 aaaagctgaa attccatcta aatcttctga aaactcttca tggttttgga atctttggac
     1141 tcgttttaca acttttgtca aaggtatttt tagctaagat ttctattttt cgttgacttc
     1201 atatggatag agaacagtgg ttcatgtgag cctttagtca aatgaaaagc gtataaaata
     1261 ttggagagat ctaggcatct aaactagaga ttgtgcaaga aattatgtaa tagacctcac
     1321 attttttgcg aatgagatgt tgacacgaat tccatatttc ttgagtctcg gttgtaattt
     1381 ttct
//
LOCUS       DASFSW010000260         1383 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.263, whole genome shotgun sequence.
ACCESSION   DASFSW010000260 DASFSW010000000
VERSION     DASFSW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.263"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..374)
                     /gene="mreD"
                     /locus_tag="U1099_002207"
     CDS             complement(<1..374)
                     /gene="mreD"
                     /locus_tag="U1099_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEM4868472.1"
                     /translation="MRNKMIEIFMFPILFFILLLDGQISTLVTNWSVGLFTISSHLVL
                     MLAIFYANYVSLGFSLFIFTLLGLVYDISYLNLIGIATTTLPLVLYCIYFFFQGAVSK
                     RGINILILLVAIFQFEFISYSFA"
     gene            complement(364..1200)
                     /gene="mreC"
                     /locus_tag="U1099_002208"
     CDS             complement(364..1200)
                     /gene="mreC"
                     /locus_tag="U1099_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEM4868473.1"
                     /translation="MNKFSKLVVVVSIFLLLSFSLLFVTFSKGLQVPYLNNIVRVVVT
                     PIQSVISVPTRFFSEQKDVLTDLMNAYEENKQLKEAIMSLEGMAAENTSLKEENASLR
                     SSLGVVSDFPEKQLIPGSVLVRTPSSWSEHISINIGETRGVTSNALVVANGGLVGIVS
                     SLSSDSAVVTLFTNSDEFTKLPVKISVDSKEIYGILSGYDADTNSFIINQLNSADEIA
                     VGSNVVTSDLAGATPANVQIGKVLSVKSNSNSLNREVYVEPTASFSNIYSVLVVGQTN
                     AQ"
     gene            complement(1260..1347)
                     /locus_tag="U1099_002209"
     tRNA            complement(1260..1347)
                     /locus_tag="U1099_002209"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1311..1313),aa:Ser,seq:gct)
BASE COUNT          525 a          284 c          197 g          377 t
ORIGIN      
        1 gcaaatgaat aactaataaa ttcaaattga aaaatagcta ctagtaaaat caaaatatta
       61 attcctcgct tgctgacagc accttgaaag aagaaataga tgcaatataa aactaaagga
      121 agagttgtag tagcaatacc aatcagatta agataactaa tatcatatac caaacctagc
      181 aaagtaaata tgaataatga aaaaccaaga gatacatagt tagcataaaa aatagctaac
      241 ataagtacca aatgacttga aatagtaaat aatccgaccg accaatttgt taccaacgtt
      301 gaaatctgtc catcaagtaa taaaataaag aacaagatgg gaaacatgaa tatttctatc
      361 attttattgc gcatttgttt gacctaccac taaaaccgaa taaatgtttg aaaaactagc
      421 tgtcggctca acatatactt ctctatttaa actgttacta tttgatttaa ccgataaaac
      481 tttaccaatt tggacatttg ccggagtcgc accagccaaa tcactcgtta caacattgct
      541 acccactgca atttcatcag ctgagttcaa ctgattaatg ataaaactat ttgtatctgc
      601 atcatagccc gataaaatac cataaatttc tttagaatca acagaaattt tcacaggcaa
      661 cttcgtgaat tcatccgaat tcgtaaataa ggtaacaacc gccgaatctg aactcaatga
      721 actaacgatg ccaactaatc caccattagc aaccacaagt gcattagatg tcacacccct
      781 agtctcacca atattaattg aaatatgctc cgaccacgat gaaggagtcc tcactaaaac
      841 tgatcctgga ataagttgtt tttcaggaaa atcagatacc acacctaaac tgctacgaag
      901 cgatgcattc tcttctttca agctagtatt ttcagcagcc atcccttcaa ggctcataat
      961 agcttccttt aattgtttat tttcctcgta agcgttcatc aaatctgtca agacatcttt
     1021 ctgctcagaa aaaaatctag taggtactga aataaccgat tgaattggcg ttacaacaac
     1081 cctaacgata ttatttaaat atggtacctg taatccctta gagaaagtca caaacaaaag
     1141 agaaaatgac agtaataaaa agatagaaac aactactact aattttgaaa atttattcat
     1201 aatggatcct cactaagata caataaaacg agaatatcaa gcgataatct cgttcactac
     1261 ggagaataag ggattcgaac ccttgcgcca gttacccgac ctaacgattt agcaaaccgt
     1321 cctcttcagc ctcttgagta attctccatc aatataatat gggcacgagt ggaatcgaac
     1381 cac
//
LOCUS       DASFSW010000261         1305 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.264, whole genome shotgun sequence.
ACCESSION   DASFSW010000261 DASFSW010000000
VERSION     DASFSW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.264"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            131..511
                     /locus_tag="U1099_002210"
     CDS             131..511
                     /locus_tag="U1099_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868474.1"
                     /translation="MSFINQFKSQSKTVLAAVAISAATLGFAGGMSISKSSTGVSTQS
                     QASLAQNSSAAMNGQGAPQGMPPGKGGPSQGMPPGQGTPPHDATSGATQSNSSTSSDE
                     VNALKKKNQEIKAQISGDSSSSNQ"
     gene            705..>1305
                     /locus_tag="U1099_002211"
     CDS             705..>1305
                     /locus_tag="U1099_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM4868475.1"
                     /translation="MIKILLVEDDLSLSNSVFDFLDDFADVMQVFDGEEGVYEAETGV
                     YDLILLDLMLPEKDGFQVLKELREKGVTTPVLITTAKESLEDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRAGKFNENTLTFGDVTVDLSTNMTMVNGEEVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRIWGFDSDTTISVVEVYVSKIRKK"
BASE COUNT          427 a          196 c          269 g          413 t
ORIGIN      
        1 gacttttata aaaaatgctg aatttcttta gcttgtttta agttttcaat actatactat
       61 tatcatcagg tgaagaggag ttatttaaca ggtctcattc tgataatatt ctattgaaaa
      121 ggggaatttc atgtcattta ttaatcaatt taagtcacag tcaaaaacag tactagctgc
      181 tgttgctatt tcagctgcta ccttgggctt tgcaggggga atgagtattt cgaaaagctc
      241 aacaggagtt tcaacacaaa gtcaggcaag tttggcgcaa aatagttctg cagcaatgaa
      301 tggacaagga gctccccaag gcatgccacc aggtaaagga ggtcctagcc aaggtatgcc
      361 acctggacaa ggtacacccc ctcatgatgc aacaagtgga gcaactcagt caaactcgag
      421 tacttcttct gatgaagtga atgctttaaa aaagaagaat caagaaatta aagctcaaat
      481 ttcaggagat agtagttctt ctaatcaata aatattgaga tttcgatttt tacttattcc
      541 atgatataat gaactcgtat caataaatag ttagataaag actaccttat cattcatttt
      601 tcagatggtc atagtagtac tttatctaac catttctctt aaagaccgtg acctgtccca
      661 ggtctttggt ttgtttatta aaccgaaacg taaggaagtg tatgatgatt aaaatattgt
      721 tagtggaaga tgatttaagt ctatcaaatt ctgttttcga tttcttagat gattttgcgg
      781 atgttatgca ggtttttgat ggtgaagagg gcgtttatga ggcggaaaca ggcgtttatg
      841 atttgattct tttggatttg atgttgcctg aaaaagatgg cttccaagtc ttgaaagaat
      901 tacgtgaaaa aggtgttacg acacctgttc tgattacaac agccaaggaa agtctagagg
      961 ataaaggcca tggttttgaa ttgggggctg atgactattt gacaaaaccg ttttatttag
     1021 aagaattgaa gatgcgaatt caagctctat taaaacgagc gggtaagttt aatgaaaaca
     1081 cactaacctt tggagatgtg acagttgacc tatctacaaa tatgacgatg gtaaatggcg
     1141 aagaagttga actgctagga aaagaatttg atctactagt ttatttcttg caaaatcaaa
     1201 atgttatttt gccaaaaaca caaatttttg accgtatttg gggctttgac agtgatacta
     1261 caatttcagt agtagaagta tatgtttcta aaattaggaa aaagt
//
LOCUS       DASFSW010000262         1299 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.265, whole genome shotgun sequence.
ACCESSION   DASFSW010000262 DASFSW010000000
VERSION     DASFSW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.265"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(13..336)
                     /locus_tag="U1099_002212"
     CDS             complement(13..336)
                     /locus_tag="U1099_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868476.1"
                     /translation="MKRFLEVIFEQITVFLEHGKAPKSIEPDSKEEIKNNLELALYKK
                     AAVHVIYGDRSFTGDIVKYDTDCQRIIMKNFKRQVTSIIKIEDIKKVTLVPDSIKTSQ
                     QQTKG"
     gene            428..643
                     /locus_tag="U1099_002213"
     CDS             428..643
                     /locus_tag="U1099_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEM4868477.1"
                     /translation="MAEFTFEIEEKLLVLSENDKGWTKELNRVSFNGAPAKYDIRTWS
                     PDHTKMGKGITLSNEEFQVLLDAFANK"
     gene            complement(655..1194)
                     /locus_tag="U1099_002214"
     CDS             complement(655..1194)
                     /locus_tag="U1099_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEM4868478.1"
                     /translation="MKTTSIKSLVYIAIGTALISTLSQISLSIGPVPFTLQTLAIGLV
                     ACLYKPKEAITSVSLYLVLGAIGLPVFSGFSGGFAALTGPTSGFLWGFLLYTIITSAL
                     TKSDSSPVTIFLACLLGTSICFLLGCFVFKLVLGASWTDSIGWTVLPFILPDLAKITL
                     VMICHRLLQPVIKKEAYFA"
BASE COUNT          435 a          216 c          269 g          379 t
ORIGIN      
        1 aaggctgaaa tttcagcctt ttgtttgttg ttgagaagtc ttgatgctat ctggaaccaa
       61 ggtgactttt ttgatgtcct cgatttttat aatgctggta acctgacgtt tgaaattttt
      121 catgataatc cgttggcaat cggtatcata ttttacaatg tcacctgtga aactacgatc
      181 gccatagatg acatggactg cagctttttt atataaagcc agctccaagt tattcttgat
      241 ttcttccttg ctatctggct caatagactt aggagctttg ccatgttcta aaaagacggt
      301 gatctgctcg aaaataactt ctaaaaacct cttcatggca taatcctttc aaacttgata
      361 taattatatt atctaatttt tataaaaatg acaagctttt tcgaatagat aagtgaggag
      421 gtaacaaatg gcagaattta catttgaaat tgaagaaaaa ttattggtct tatctgaaaa
      481 tgataaagga tggaccaagg agcttaatcg agtatctttt aatggcgcac cagccaagta
      541 tgacatccgt acctggagtc ctgaccatac caagatgggg aaaggaatca cacttagtaa
      601 tgaggaattt caagttctac tcgatgcgtt tgccaataaa taaacaaagg aagcctaagc
      661 aaaataggct tcttttttga taacaggttg caataaacga tgacaaatca tgactagtgt
      721 aattttagct aaatcaggaa gaatgaaagg cagaacagtc catccgatag agtcagtcca
      781 actggcacct aaaactagct taaatacaaa acagccgaga aggaaacaga tacttgttcc
      841 aagtaggcag gctagaaaaa ttgtgactgg tgaggagtct gatttagtga gggcagaagt
      901 gatgatagtg tagagtagaa acccccatag aaagcccgag gtcggtcctg tgagtgcagc
      961 aaagccacca gaaaaaccag aaaaaacagg taaaccgata gcaccaagaa ctagatataa
     1021 gctgacactt gtaattgcct ccttgggttt gtatagacaa gctactaagc cgatagctag
     1081 ggtttgtaat gtaaatggta caggtccgat agacagactg atttgtgaaa gggtggaaat
     1141 taaggctgtt ccaatagcga tataaacaag tgatttgata gaagttgttt tcatttgtta
     1201 tcctcttaaa attttatggt taacaaaaat tatagtgaga tttgataaga aagtcaatgc
     1261 tctctcatcg gctgtaacaa ctctataggt gatttttat
//
LOCUS       DASFSW010000263         1294 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.266, whole genome shotgun sequence.
ACCESSION   DASFSW010000263 DASFSW010000000
VERSION     DASFSW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.266"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            37..>1294
                     /locus_tag="U1099_002215"
     CDS             37..>1294
                     /locus_tag="U1099_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEM4868479.1"
                     /translation="MGKFGVMYTRSKEYGRPSKLFYKSFDSFTEEELFEENECSFRLK
                     IVHIDSNNCFVKSVDFQKGRIFLYSFDRTGFVRHSYTETIAPIPRDIALFSTEKAQYF
                     LGLTSTEEKKDQLILRETFSGDRVSIILPYQDRARRVHCIGRFILLDCSNAASSVFYL
                     ATFKNNSLRELSVNKITFDEKTTLYENSFSDRVLLFLERRTFYEKILSFDLIENTLKT
                     EFERPIIKSNNTKYFSKVIWTKKDSYDVSIPISLFWKSEDTDELPRRKKCILSVYGAY
                     GKNDNSDLDEIMLSIIDAGFIYAIVHVRGGGYLGGEWYRSGKALNKWNSIRDFIEGVN
                     YLRENDVIDSKRLGLITSSAGGIIAGAVLNEEKNLLQSILLFSPFINPYDTLQNPNDP
                     LSKTEIAEWGDIRAPEVRAYIKSYSPM"
BASE COUNT          436 a          178 c          261 g          419 t
ORIGIN      
        1 atcattgaag atatttcagt tgaaagttcg ttctacttgg gtaaattcgg tgttatgtat
       61 actcgctcca aagagtacgg gaggccaagt aagttattct ataaatcttt tgatagcttt
      121 acagaagagg aactgtttga agaaaacgaa tgctcttttc gattaaaaat agtccacatt
      181 gatagcaata attgttttgt aaagtcagta gactttcaga aaggcaggat ttttctatac
      241 agtttcgacc gaacaggttt tgttagacac tcttacacag aaacgatagc cccaatacct
      301 agagatatcg ctctatttag tacagaaaaa gcacagtact ttcttggact cacttcaact
      361 gaagaaaaga aagatcaact aattctaagg gaaacttttt cgggagacag agtttctatt
      421 attctaccat atcaagatag ggctagaaga gttcactgta taggacgttt tatcctttta
      481 gactgttcaa acgcagcgag ttccgtcttt tatcttgcta catttaaaaa taatagtttg
      541 agggagttga gtgttaataa gataacattt gatgaaaaga caacacttta tgaaaattca
      601 ttttccgatc gtgttcttct atttttagag aggagaactt tttacgaaaa gatacttagt
      661 tttgatttaa tagaaaatac attaaaaact gaatttgaaa gacccatcat aaaatcgaat
      721 aatactaaat atttttctaa ggtgatttgg actaaaaaag acagttatga tgtaagcata
      781 ccgatttctt tgttttggaa aagtgaagat acggatgagc ttccaagaag aaaaaaatgt
      841 attctaagtg tctatggggc ttatggtaag aatgataatt ctgatttaga tgaaattatg
      901 ttatctataa tagacgctgg ttttatttat gctattgttc atgtaagagg tggaggctat
      961 ttgggaggtg aatggtatcg ctcaggaaag gcgttaaaca aatggaattc tatcagagat
     1021 tttattgagg gggtaaatta tttaagggaa aatgatgtaa ttgacagtaa acgattaggt
     1081 ttgataactt caagtgcagg tggaataatt gctggtgcgg tgttgaacga ggaaaaaaat
     1141 ttattgcaaa gcattctctt attttcgcca tttataaatc cttatgatac acttcagaat
     1201 ccaaatgatc ctctttctaa gaccgaaata gcagagtggg gggatattag agcccctgaa
     1261 gtaagggctt atataaagtc ttattcgcct atgc
//
LOCUS       DASFSW010000264         1268 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.268, whole genome shotgun sequence.
ACCESSION   DASFSW010000264 DASFSW010000000
VERSION     DASFSW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.268"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..991
                     /locus_tag="U1099_002216"
     CDS             <1..991
                     /locus_tag="U1099_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM4868480.1"
                     /translation="AIFMMIQSIRSGKLLVGNFVSLIQAITRVNTYSQSMIQNTYIIY
                     NTSLFMEQLFKFFDMEVSNISIQNSRISRIEKRIDKIKVNNLSFIYPGSVKKTLEDIN
                     VEFNKGELVAIVGKNGSGKSTLVKLLSGLYQPSEGQIYYNNESSDVLDLSYYQNNVSV
                     LFQDFVKFELSVRENIGLSDVNSISDSKIKKHIDNLKLNFLTAENNFNLNQRLGTWFN
                     DSRQISGGQWQKVALARTFFKNASIYILDEPSSALDPVSEKEIFDEFVTRSRDKIALF
                     VSHNLMAASRADRIIVMQDGRIIDEGKHDDLISKSKYYRELYYSEKYEEESDG"
     gene            984..>1268
                     /locus_tag="U1099_002217"
     CDS             984..>1268
                     /locus_tag="U1099_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEM4868481.1"
                     /translation="MDENKVIIDLSEKVFAKFDEQLKRYAEQPNYDLLTLSSGLPGLI
                     LLSSELTSLTSERKYSARTGKYVNFMVKQMRNYGVLSDSLFSGVSGIGISI"
BASE COUNT          455 a          160 c          234 g          419 t
ORIGIN      
        1 agctattttt atgatgatac agtcaattcg aagcggtaaa cttttagtag gtaattttgt
       61 aagccttatt caagcaataa ctcgtgtaaa tacctactca caatctatga tccaaaatac
      121 ttatatcatc tataacacaa gtttattcat ggaacaatta tttaaatttt ttgatatgga
      181 agtaagtaat atttcaattc agaatagtag aatctcaaga attgaaaagc gcattgacaa
      241 aataaaagtc aataatctct cttttatcta tccaggaagt gtcaaaaaaa ctttagagga
      301 tattaatgtt gagtttaata agggtgaact tgtagcaatt gtggggaaga atggttctgg
      361 taagagtacc ttagtaaaat tactatcggg attataccaa ccttcagagg gtcaaatata
      421 ttataataac gaatctagtg atgtcctaga cttgagttat tatcaaaata atgtttctgt
      481 tctttttcag gactttgtga agtttgaatt atctgtgaga gaaaatattg gcctaagtga
      541 tgtaaacagt atatctgact ccaaaataaa gaaacatatt gataacttaa aattaaattt
      601 cttaaccgca gaaaacaatt tcaaccttaa tcaacgttta gggacctggt ttaacgatag
      661 tagacaaatt tcaggaggac aatggcaaaa agtagcattg gctaggactt tcttcaagaa
      721 tgcaagcata tacattttgg atgaaccaag ttcagcgctt gatcctgttt cagagaaaga
      781 aatatttgat gaatttgtaa ctcgttctag agataagatt gctttgtttg tgtcacataa
      841 tctaatggct gcgagtagag cagatcgaat tatagttatg caagatggac gaattattga
      901 tgaaggaaaa catgacgacc ttataagtaa atctaaatat tatagggaac tttactattc
      961 agaaaagtac gaggaggagt ctgatggatg aaaataaagt tattattgat ttatctgaga
     1021 aagtatttgc taaatttgat gagcagttaa aaagatatgc tgaacaaccg aattatgatt
     1081 tattgacctt gtcttctggt ttacctggat tgatattatt atcttcagaa ttgacaagtt
     1141 taactagtga aagaaaatac tccgctagaa cgggtaaata tgttaacttt atggttaaac
     1201 aaatgagaaa ttatggtgtt ttatcggatt ctttattttc aggagtttct ggaattggaa
     1261 taagtata
//
LOCUS       DASFSW010000265         1266 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.269, whole genome shotgun sequence.
ACCESSION   DASFSW010000265 DASFSW010000000
VERSION     DASFSW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.269"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            51..1160
                     /locus_tag="U1099_002218"
     CDS             51..1160
                     /locus_tag="U1099_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEM4868482.1"
                     /translation="MKKQVIIIGGGIVGSTVAFYLSQEEQVNLTLIDSGIGTATRAAA
                     GIICPWMAQKKNKDWYKLTSDGAVFYRQLVADLEKSGATEIPFKQTGTIGLKSKPELL
                     DKIQKIAEERRVDTPTIGEITPLIGSEINQYLPLLKPDYFGIHLEGGGRIDGGRLIDI
                     LQEQFLKNGGTRIYGQAKLLDNHTVEVDNQVLTADHIILATGAWLPHILEPLGYQVDV
                     RPQKGQLFELDTEFDTDNWPVCMPYGEIDILPFENGKIIIGATHEDDMGYDLELDPEK
                     IQAMHDKIAEFMPDLANYPVARTRIGTRAYTSTYSPFYGNIEDMPNVWVASGLGSTGL
                     TNGPIIGWQIAKEILNQETNFDRTPYSPNRYILKK"
BASE COUNT          411 a          254 c          250 g          351 t
ORIGIN      
        1 tcatgttgaa aaaaataaat aaacttccga atttgtgata taattaactt atgaaaaaac
       61 aagttattat tataggcgga ggaattgttg gctctactgt agccttttat ctctcccaag
      121 aagaacaagt caatcttact ctcattgatt caggtattgg aacagccaca cgagctgccg
      181 caggtatcat ctgcccatgg atggctcaga aaaaaaataa agactggtat aagctgactt
      241 ccgatggtgc tgttttctac cgtcaattgg tagccgattt ggaaaaatcg ggtgctactg
      301 aaattccatt caaacagact ggaaccatcg gcctaaaaag taagcctgaa ctacttgata
      361 aaatacaaaa aattgccgag gaacgtcgcg tagatactcc gactattggc gaaattactc
      421 cgcttatagg ctcggaaatt aaccagtacc tgccactact taagccagac tattttggta
      481 tccatttaga aggtggcggt cgtattgatg gtgggcgttt gattgatatt cttcaggaac
      541 aatttttgaa aaatggtggt acacgtatct acggacaagc aaaactgctc gacaaccata
      601 ctgtcgaagt agataatcaa gtgcttactg ctgaccatat tatcttagcc acaggggcct
      661 ggttgccaca tattcttgaa cctcttggtt atcaagtgga tgttcgacca caaaaaggac
      721 aattgttcga attagataca gaattcgata ccgacaattg gccagtttgt atgccttacg
      781 gagagattga catcctacca tttgaaaatg ggaaaatcat tattggtgca actcatgaag
      841 atgacatggg ctacgattta gaactagacc ctgaaaaaat ccaagccatg catgataaaa
      901 tagctgaatt catgcctgat ttggcaaact atcctgtagc cagaactcgc attggaacgc
      961 gcgcctatac ttcaacttat tcaccattct acggaaatat tgaggatatg cctaatgtct
     1021 gggtagctag tggtctgggc tccaccggtc tgacaaacgg tccaattatc ggttggcaaa
     1081 ttgctaaaga aattctcaat caagaaacaa actttgaccg tacaccatat tctccaaata
     1141 gatatattct caaaaaataa gatacgatac gtttagattc tactaactct ttaaatgtag
     1201 atactaagac tagttgttat ctaagcaata aatcatgacc acccgtcaaa cgggtggttt
     1261 gaacaa
//
LOCUS       DASFSW010000266         1256 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.270, whole genome shotgun sequence.
ACCESSION   DASFSW010000266 DASFSW010000000
VERSION     DASFSW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.270"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            31..1053
                     /locus_tag="U1099_002219"
     CDS             31..1053
                     /locus_tag="U1099_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEM4868483.1"
                     /translation="MLEHSIDIQLKHPDDSFSLFGSNERHLRLMEQELGVVIHARTEK
                     VQVIGQEEHVEQARLVIQSLLVLVSRGLVINTPDVVTAISMAKNDEIEKFVALYEEEI
                     IKDNYGKPIRVKTLGQKLYVDSVKRHDIVFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IVLTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTTRLMEREIIEIAP
                     LAYMRGRTLEDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDISQIDLPRKVKSG
                     LIDATEKLSKIPQIDFVHFSAKDVVRHPVVAQIINAYEQEALAADGRASFETIGEPSR
                     KYNGEE"
BASE COUNT          373 a          253 c          309 g          321 t
ORIGIN      
        1 tagagaaaag cacaggaaaa ggagcaactc ttgctagaac attcaattga tattcaactc
       61 aagcatccag atgattcgtt tagcctattt ggttcgaatg aacgccatct gagattgatg
      121 gaacaggaac taggtgtggt tattcatgcc cgtacggaaa aagtgcaggt gattggacag
      181 gaagaacatg tagaacaggc acgtcttgtt atccagtctc tccttgtctt ggtgagtcgt
      241 ggtttggtta tcaatacacc agatgtggtc acagctattt ccatggcaaa aaatgatgag
      301 attgagaaat tcgttgccct ctatgaagaa gagattatca aggacaacta tggcaaaccc
      361 attcgcgtaa agactcttgg acaaaaactc tacgtggaca gcgtaaagcg tcatgacatc
      421 gtgtttggaa ttggaccagc tggtacaggg aagaccttct tggcagttac tctagctgta
      481 acagccctca agcggggaca ggtcaagcgg attgtcctga cacgtcctgc ggtcgaggca
      541 ggcgaaagtt taggcttttt accaggggac ttaaaagaaa aagtagaccc atatttgcgt
      601 cctgtctatg atgccctcta ccagattttg ggcaaggagc agaccacgcg cctaatggag
      661 cgcgagatta tcgagattgc accgttggcc tatatgcggg ggcggacctt ggaagatgcc
      721 tttgtcatct tggatgaggc gcaaaatacc accattatgc agatgaagat gttcctgacc
      781 cgtctgggct ttaattccaa gatgattgtc aatggggata ttagtcagat tgacctgcca
      841 cgcaaggtta agtcaggcct gattgatgcg acagagaaac taagcaaaat tccgcagatt
      901 gatttcgtac atttttcagc caaggatgtg gttcgtcatc cagttgttgc ccagattatc
      961 aatgcctatg agcaggaggc tctggctgcg gatggtcgag caagttttga gacgattggg
     1021 gagcctagtc ggaaatacaa tggcgaagag tagttgtttc attggctgat atataaaaga
     1081 attgtgatgc tagttacatt taatatacaa taaaaagccc aaatggacga tactgtaagc
     1141 gatgaaacaa acaggaactt agtccaaata agcgacttac agcatatcgt aaaatcacat
     1201 cgtacagcaa atatcaacta tttgtcacaa gttataccaa tcctattgcc cgaccc
//
LOCUS       DASFSW010000267         1221 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.271, whole genome shotgun sequence.
ACCESSION   DASFSW010000267 DASFSW010000000
VERSION     DASFSW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.271"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..1134
                     /locus_tag="U1099_002220"
     CDS             <1..1134
                     /locus_tag="U1099_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEM4868484.1"
                     /translation="LMAVSFMLENNFALSRRGAVYFTLINFVLVYLFNVIIKQFKDSF
                     LFSTTYQKKTILITTAELWENMQVLFESDILFQKNLVALVILGTEIDKINLPLPLYYS
                     VEEAIEFSTREVVDYVFINLPSEYFDLKQLVSDFELLGIDVGVDINSFGFTVLKNKKI
                     QMLGDHSIVTFSTNFYKPSHILMKRLLDILGAVVGLIICGIVSILLIPIIRRDGGPAI
                     FAQKRVGQNGRIFTFYKFRSMFVDAEVRKKELMAQNQMQGGMFKMDNDPRITPIGHFI
                     RKTSLDELPQFYNVLIGDMSLVGTRPPTVDEFEKYTPSQKRRLSFKPGITGLWQVSGR
                     SDITDFNEVVRLDLTYIDNWTIWSDIKILLKTVKVVLLREGSK"
BASE COUNT          407 a          171 c          243 g          400 t
ORIGIN      
        1 cttatggcag tttcatttat gttagagaat aatttcgcac tttcaagacg tggtgccgtg
       61 tatttcacat taataaactt cgttttggta tacctattta acgtaattat taagcagttt
      121 aaggatagct ttctattttc gacaacctat caaaaaaaga cgattctaat tacaacggct
      181 gaactatggg aaaatatgca agttttattt gaatcagata tactatttca aaaaaatctt
      241 gttgcattgg taattttagg tacagaaata gataaaatta atttaccatt accgctctat
      301 tattctgttg aagaagctat agagttttca acaagggaag tggtcgacta cgtctttata
      361 aatttaccaa gtgaatattt tgacttaaag caattagttt cagactttga gttgttaggt
      421 attgatgtag gcgttgatat taattcattc ggttttactg tgttgaagaa taaaaaaatc
      481 caaatgctag gtgaccatag catcgtcact ttttccacaa atttttataa gcctagtcac
      541 atcttgatga aacgactttt agatatactt ggagcagtag tcgggttaat tatttgtggt
      601 atagtttcta ttttgttaat tccaattatt cgtagagatg gtggaccagc catttttgct
      661 cagaaacgag ttggacagaa tggacgcata tttacattct acaagtttcg ttcgatgttt
      721 gttgatgccg aggtacgtaa gaaagaatta atggctcaaa accagatgca aggtgggatg
      781 ttcaaaatgg acaacgatcc tagaattact ccaattggac acttcatacg aaaaacaagt
      841 ttagatgagt taccacaatt ttataatgtt ctaattggag atatgagtct agtcggtacc
      901 cgtccaccta cagttgatga atttgaaaaa tatactccta gtcaaaagag aagattgagt
      961 tttaaaccag ggattacagg tctttggcaa gtgagcggaa gaagtgatat cacagatttt
     1021 aatgaagtcg ttaggctgga cctaacatac attgataatt ggaccatctg gtcagacatt
     1081 aagattttat tgaagacagt gaaagttgta ttgttgagag agggaagtaa gtaagactcc
     1141 tttaaaacaa agaatagtaa gtagtagggg atatgagaac agtttatatt attggttcaa
     1201 aaggaatacc agcaaagtat g
//
LOCUS       DASFSW010000268         1200 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.272, whole genome shotgun sequence.
ACCESSION   DASFSW010000268 DASFSW010000000
VERSION     DASFSW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.272"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            210..>1200
                     /locus_tag="U1099_002221"
     CDS             210..>1200
                     /locus_tag="U1099_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM4868485.1"
                     /translation="MDVLYQSCAGIDVHQANIVVCILHGPLTSTRPKREMATFDTTTK
                     GLRACHDFLSQFHVEAVGMESTGVYWRPVWHALCDDFKLILAQPAHMKAIPGQKTDKK
                     DAHWIAKLTRIGLLPRSFVPDEAIQELRELTRQRKHYVESRNRETNRIHKILQSGGIK
                     LTTYIEDIMGLSGRNLLQLLVDGTPITPRIVHQSVYTSLKKKVPQLLEALDGYFSDHH
                     RFMLKQSLEIYDFYQKQIELLEERMNVYLSQYENHVEILDSIPGIDVITASVIISEVG
                     VDMSQFPTAGHLASWAGLCPGNNESAGKKRSTKIRHGNSYLKKCLCQAAFAIKK"
BASE COUNT          356 a          246 c          259 g          339 t
ORIGIN      
        1 cagaataatg aaaacgaaca taaagccctc actaggtgta taatcttagt gagggctttg
       61 tggttgacga gttactgatg ttagtcatcg cgggaaaaac taacccactc tactgacgag
      121 caccccaaaa tatgcgatag gcatgagtat aaaaatacga ctaaaattcg gtagataagc
      181 tctctaattt ttagaaggag agaattatca tggatgttct ctatcaatct tgtgcaggta
      241 ttgatgtcca tcaagccaat attgtcgtct gtatcctaca tggaccactc acctcaactc
      301 gtccaaagcg tgagatggct acatttgata caacgactaa aggcctacgt gcttgccacg
      361 attttctcag tcaatttcat gtggaagctg ttggtatgga aagcacaggt gtttattggc
      421 gacctgtctg gcatgctcta tgtgatgact tcaagttgat actcgctcaa ccagcccaca
      481 tgaaggctat tccaggtcag aaaaccgaca agaaggatgc tcactggatt gccaaattaa
      541 cacggattgg tctattgcct cggagtttcg tccccgatga agctattcaa gaattgaggg
      601 aattgaccag acagcgaaaa cattatgtgg aaagtcgcaa tcgcgaaacc aaccgtatcc
      661 ataaaattct tcagtcaggc ggtatcaagc taacaaccta tattgaagat attatggggc
      721 tatctggtcg caatctcctt cagctactgg ttgatgggac gcctattaca cctcgcattg
      781 tccatcaatc tgtttatacc agcttgaaga agaaagtgcc gcagcttctg gaagccttgg
      841 atggctattt ttctgaccat caccgcttta tgttaaagca gtccttggag atttatgatt
      901 tttatcagaa gcagattgag ttgttggaag agcgaatgaa tgtctatcta tcacaatatg
      961 aaaaccatgt agaaatattg gattccatcc caggcattga tgtcattaca gcttctgtta
     1021 tcatttctga ggttggtgtt gacatgagtc aatttccaac tgctggacat ctagcttctt
     1081 gggcaggact ttgtccaggt aataatgaga gtgctggtaa aaaacgaagt accaagattc
     1141 gacacggaaa ttcatatttg aagaaatgtt tatgccaagc cgctttcgct atcaaaaaac
//
LOCUS       DASFSW010000269         1197 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.273, whole genome shotgun sequence.
ACCESSION   DASFSW010000269 DASFSW010000000
VERSION     DASFSW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.273"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..732
                     /locus_tag="U1099_002222"
     CDS             <1..732
                     /locus_tag="U1099_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HEM4868486.1"
                     /translation="HEFEVELTEHQIEKLVDEGIYIKVSFGIKQSGLVFIPNYQLDII
                     EEDNQKKYNVYICETTSYFVYNKEYSDKNQYIKGRTLIRQLTNDSRMIPYRRSTVEKL
                     QEKISEINLLIELTETDKKYQDIKDDLVTKIAELDIKLNQTNKKIATLNKMAEVLINL
                     KSEEHESRKLARYDFSKLNMTESTSLEQINAELLRLQKELSIFLYEYEDVVRRLETFR
                     KILNRQGLNWRNGHDREVDKYEIID"
BASE COUNT          474 a          142 c          219 g          362 t
ORIGIN      
        1 cacgaatttg aagtggaact tacagagcac caaatagaga agctagttga tgaggggatt
       61 tatatcaagg tgtcttttgg tattaagcag agtggccttg ttttcattcc caattatcaa
      121 ctagatatta ttgaagaaga caatcagaaa aaatataacg tttatatttg tgagacaact
      181 tcatactttg tctataacaa agaatactcg gataagaatc agtacatcaa agggcgaacc
      241 ttgattagac agttgactaa tgatagtcgg atgatacctt acagaagatc aacagttgaa
      301 aaacttcaag aaaagatttc tgagattaac ctcttgattg agttaactga gacagataag
      361 aaatatcaag acattaaaga tgacttggtt accaaaatag cagagctaga tatcaaacta
      421 aatcaaacta ataaaaaaat cgccacctta aataagatgg cggaagtgct tatcaatttg
      481 aagagtgaag aacatgagag tagaaaatta gcaaggtatg acttttcaaa actgaatatg
      541 acagaatcaa catctttaga acaaatcaat gcggaacttt tgaggttaca aaaggagtta
      601 agtatctttc tttatgagta tgaggatgtt gtaagaagat tagaaacatt tagaaagata
      661 ctaaataggc aagggttaaa ttggagaaac ggtcatgata gagaagttga caaatatgaa
      721 ataattgatt aatttcttta tatagcatat agcttccaac aaatacataa agtaaatttt
      781 gttttagata gattgtcttc atcataaatt tattagataa gaggaggatt gttaaataat
      841 aatggagaaa aatttttgat gagtttgctg tatatatagt cacgccaaac gcatgaagaa
      901 aattttgttt ttagaaatcg aaaaacatac tctaaaatca atcaacagtt gatagagctc
      961 gtaatgttct cgtagttctt gagaatagcc gattgaatcg attagaaaat cagattttaa
     1021 tatcaataat gattcttttc aagatgttaa aaaagggtct gagcatgatt tcaatattcg
     1081 ttataatagt aagacatttt ttcggtaaaa cagataaaag ctgaatcagt tagttcagtg
     1141 aagatttctt caaattattc agaaaataat ttattggttt taagagaaga aaaatga
//
LOCUS       DASFSW010000270         1175 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.274, whole genome shotgun sequence.
ACCESSION   DASFSW010000270 DASFSW010000000
VERSION     DASFSW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.274"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            241..378
                     /locus_tag="U1099_002223"
     CDS             241..378
                     /locus_tag="U1099_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEM4868487.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWER
                     C"
     gene            668..>1175
                     /locus_tag="U1099_002224"
     CDS             668..>1175
                     /locus_tag="U1099_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868488.1"
                     /translation="MNNDEWIYQYPIGKFVERQGWKIHVSSEYNSSHELLQDVAKICH
                     EMGIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPQFKIPKGIVP
                     PGFLNKWLD"
BASE COUNT          396 a          153 c          241 g          385 t
ORIGIN      
        1 aagggcctca actattccga ataaattatt cagaatagtt gagaaaccca taatcttaga
       61 tataaggtca tctcccgtat ctattataat tcaggaggtt ctaatttatg aaaatctcac
      121 gagaaaacgt cattttctat gtaaaacatt gtataattgt ttgcatttta ctgttggaga
      181 gagtataatg agagtggata aagtgcttaa tcccaaaaat ttaaacagga ggaactagct
      241 atggcagaag aagtattaaa tcttcaatta gtatctgttc aagttgacga aacagatgaa
      301 gttgatggaa tgcgatttag tacattcagt acaaaccgtt gcggtaactg gagtgctttt
      361 agttgggaaa gatgttaact catttaaatt gtaaacttta gagattctct ggagttatgg
      421 atgtcgttcc aatatgtgca cgttggaatg ttagtgctta tatgggggaa atccaaagga
      481 ttctcccttt ttatttcaat aagtctctct agctatagat atattaagtt tgatagaatt
      541 tcttggagat tttgttatgg attataattt caatttggag catcctttct ttttcactaa
      601 taatgattat agtacagata ctagcataaa gtaccaagta agtctgcctt ttaattggca
      661 cgaagtaatg aataatgatg agtggattta tcaatatcct attggaaaat ttgttgaaag
      721 acaagggtgg aaaatacacg ttagttcaga gtataatagt tcacacgaat tgttacaaga
      781 tgtagcaaaa atttgccatg aaatgggaat tccatttaaa catcttagta cagaagacaa
      841 atttatcatg cgaaacggga aattggttag tcgaggattt tctggtaaat ttattacatg
      901 ttatccaaat caaaatgaat tagagtcggt tttgcaaagg ttagaaagtg ccttaaagca
      961 atataatggg ccatatattt taagcgataa acgttgggat gaagcaccga tttatctgag
     1021 gtatggtgtg tttaggccgt caagggatga tgaaaagaag gttgccattg atgaattaat
     1081 tgttggcgat gaagttgtta aagatgaacg cttaccacaa tttaaaattc caaaaggtat
     1141 agtgccccct ggctttttga ataaatggct tgata
//
LOCUS       DASFSW010000271         1175 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.275, whole genome shotgun sequence.
ACCESSION   DASFSW010000271 DASFSW010000000
VERSION     DASFSW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.275"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            42..>1175
                     /locus_tag="U1099_002225"
     CDS             42..>1175
                     /locus_tag="U1099_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEM4868489.1"
                     /translation="MKKIFTKTSIYYLLSFLIPLTIISIVLAFQGIWWGSDTTILASD
                     GFHQYVIFNQTLRNTLHGDGSLFYTFSSGLGLNFYALSSYYLGSFLSPIVFFFDLQSM
                     PDAIYLVTIVKFGLTGLSTYFSLKGIHKNLKEEWALLLATSFSLMSFSTSQLEINNWL
                     DVFILLPLVLLGLHRLLKKQGPILYYITLTCLFIQNYYFGYMVAIFLTLWTLVQLSWI
                     DSQRIKRFIKFTIVSILSALSSMFMLLPTYLDLKTHGETFTKIVNLKTEDSWYLDFFA
                     KNLVGSFDTTKFGSIPMISVGLVPLILALLFFTLKEIKPTIKLSYALFFTFIISSFYL
                     QPLNLFWQGMHAPNMFLYRYAWALSITVIYLAAETLVRLRQVSI"
BASE COUNT          343 a          233 c          168 g          431 t
ORIGIN      
        1 gaccggtcaa gttctttccc acttcctttt cttttaaatc tatgaaaaaa atatttacaa
       61 aaacatccat ttattatctc ttatctttcc ttattccgct gactattatt tctatcgtct
      121 tagcatttca aggaatctgg tggggaagtg atacaacaat attggccagt gatggtttcc
      181 atcagtatgt tatttttaat caaacattac gaaatacttt acacggagac ggctctttat
      241 tttacacatt ttcaagtggt ttaggactca atttttatgc tctatcctcc tattacctag
      301 gttcattcct atctccaatt gtctttttct ttgatttaca atccatgcct gacgctatct
      361 accttgtcac tattgtcaaa tttggattga ctgggttgtc aacttatttc agtctaaagg
      421 gtatccataa aaatttgaaa gaagaatggg cactattact cgctactagt ttttctctga
      481 tgagttttag tacaagtcaa ttagaaataa acaactggct agacgttttt atacttctcc
      541 cactcgttct tcttggattg catcgattat taaaaaaaca gggtccgatt ctctactaca
      601 taacattaac atgcttgttt atccaaaact attacttcgg ttatatggtg gctattttcc
      661 taacattatg gacattggtt caattatcat ggatagatag tcagagaatc aaaagattta
      721 tcaagtttac aatagtgtca attttatctg ctctaagcag catgttcatg ttgttaccaa
      781 cctacttgga tttaaaaact catggtgaga catttacaaa aattgtcaac ctaaaaaccg
      841 aagactcttg gtatctggac ttctttgcaa aaaacttagt tggtagcttt gatacaacaa
      901 aatttggttc tattcccatg atctctgttg gtctcgttcc attaatactc gctttactat
      961 tctttacatt aaaagaaata aaaccaacta tcaaactctc ctatgctcta ttctttacat
     1021 tcattatttc aagtttctac ctacaaccac tcaatctttt ttggcaaggt atgcatgcgc
     1081 caaacatgtt cctctatcgt tatgcatggg ctctatcaat aaccgttatc tatttagcag
     1141 cagagacatt ggtacgacta cgtcaagtaa gtatt
//
LOCUS       DASFSW010000272         1163 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.276, whole genome shotgun sequence.
ACCESSION   DASFSW010000272 DASFSW010000000
VERSION     DASFSW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.276"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..890)
                     /locus_tag="U1099_002226"
     CDS             complement(<1..890)
                     /locus_tag="U1099_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEM4868490.1"
                     /translation="MVKKYIDDLSLQTAMTEYNSSMVKGNEVQDQYDRSIGYVTLDFD
                     NENGNGEQVYAVVKNPNLPKDQVLEVTVLFRGSTGPNEVLSKPADVWNDWVENDLFLG
                     LRVFAQDHPNYTQNHDHASGQLKASSEALNTIMATYPNAKVNLYGHSLGSMDVQYAIA
                     NLSDTDINRLESAYIYNGPNIYGILDDQQKARVDSVKGLIHNYADPKDPVSMVGRDLD
                     KGSLDSVGMVHFVESKDIDLGNQHMTYGYQLDSSGNIKVLQTSSTEGINGTIIEMSRF
                     QQMKQTLSRGGFSSRETIYL"
     gene            complement(897..>1163)
                     /locus_tag="U1099_002227"
     CDS             complement(897..>1163)
                     /locus_tag="U1099_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEM4868491.1"
                     /translation="SEEARKVYEDKIRREESVAFTNQGVIRTYKIDEASLEYNPMGGL
                     MVDVFINDDREAYISFNLIDNGDGTYSSAYYIISAKFNHLLEME"
BASE COUNT          316 a          259 c          215 g          373 t
ORIGIN      
        1 tccagataga tggtttcacg cgaactgaag ccaccacggg atagggtttg tttcatctgt
       61 tggaagcgac tcatttcaat gatggtccca tttatccctt ccgtggaaga agtctgtagg
      121 accttaatat tgccagaaga gtctaactga tagccatagg tcatgtgctg attgccaaga
      181 tcgatatcct tactttccac gaagtggacc atcccaacgg aatctaaact tcctttgtct
      241 aggtcacgtc cgaccatact gacaggatcc tttggatctg cataattatg aatcagccct
      301 ttgacggagt cgacccgtgc tttctgttgg tcgtctagga tgccgtatat attagggcca
      361 ttatagatat acgcgctctc taagcggtta atatcggtgt cagataaatt agcaatggcg
      421 tactggacat ccatcgaacc tagggagtgg ccgtacagat tgacttttgc atttgggtag
      481 gtagccatta ttgtatttag agcttctgag gatgctttta attgtccact cgcatggtca
      541 tgattctggg tatagttcgg atgatcttga gcaaagacgc gcaaacctaa aaaaagatcg
      601 ttttcaaccc agtcattcca cacatctgca ggttttgaca acacttcatt tggtccagtc
      661 gaacctcgaa agagaaccgt aacttccaac acttggtctt tgggcaaatt tggatttttc
      721 acgacagcat aaacctgttc accattgcca ttctcattgt cgaagtcgag ggtgacataa
      781 ccaatacttc gatcgtattg atcttggact tcattacctt taaccatact agagttatat
      841 tcagtcatag cggtttggag actgagatca tctatatatt ttttaaccat ttttctttat
      901 tccatttcta gtagatgatt aaatttagct gaaataatat aatacgccga actatatgtc
      961 ccatctccgt tatcaatcaa attaaaacta atatatgctt ctctatcgtc atttataaat
     1021 acgtcaacca taagtcctcc cattggatta tattctaaac tagcctcatc aattttataa
     1081 gttcgaatga ccccttgatt agtaaaggca actgactcct ctcttctaat tttatcttca
     1141 taaaccttcc tcgcctcctc gct
//
LOCUS       DASFSW010000273         1157 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.277, whole genome shotgun sequence.
ACCESSION   DASFSW010000273 DASFSW010000000
VERSION     DASFSW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.277"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..500
                     /locus_tag="U1099_002228"
     CDS             <1..500
                     /locus_tag="U1099_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HEM4868492.1"
                     /translation="TLLDDKKLDFSEKLYFLEKHYIFSFKELGEIFDNLSIDKDSIKT
                     LKRRDFYLLTYLSSKNLWNNFSEWEDNVWYVIDEMSDHEFISFWILQGIIFNGMDYYA
                     FDYKPADKKYTIWIKKYSFDRINEIEDYEDTVIKKIQSRLDLLEIKGYRFEKRIDERY
                     KRDYD"
     gene            679..>1157
                     /locus_tag="U1099_002229"
     CDS             679..>1157
                     /locus_tag="U1099_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HEM4868493.1"
                     /translation="MEKHRVRQHIVPQAYLKKFAQKNVNNSGANISVYQKANNRQFNC
                     AVKNVAFEKNYYDVTNKENTKYWEEHFATHIEPLYGNELTNIISSIMLSNSNENVLNP
                     SQKFSLAMMIVFQIIRVPKFLKGRFQNGEVIFDETIRELIQLMPMEFISKNIEVLED"
BASE COUNT          447 a          137 c          206 g          367 t
ORIGIN      
        1 atactttatt ggatgataag aaattagatt tttcagaaaa actatatttt ttggaaaaac
       61 attatatttt ttcattcaag gagctaggag aaatatttga taatctatcc attgataagg
      121 actcgatcaa aacactaaaa cgtagggatt tctatttgct aacttactta tccagcaaaa
      181 atctctggaa taatttttcc gaatgggaag acaatgtttg gtatgttatt gatgaaatga
      241 gtgatcacga gtttatttct ttctggatat tacaagggat tatatttaat ggcatggatt
      301 attatgcttt tgactataag ccagctgata agaaatatac tatctggata aaaaaatata
      361 gttttgatag gataaatgaa attgaagatt atgaagacac tgttataaaa aagattcaat
      421 cacggcttga tttgctagaa atcaaggggt atcggtttga aaagagaata gatgaaagat
      481 acaaacgtga ctatgattaa tatatgattt gttatgaaga cggtctcaca ttttttctaa
      541 aataataggc tgaaatattc attcgctgaa cagaatcttt tgatttatcc actggtaaaa
      601 gacggaattt agttggaatg ggctaatcaa gtaaaaaaca gaatttcatt ataaaacttt
      661 ttaaggaaga ttaagtggat ggaaaagcat agagttagac aacacatagt tcctcaagca
      721 tatttaaaaa aatttgcaca aaagaatgtc aacaatagcg gagcaaatat atctgtctat
      781 caaaaagcaa ataatagaca gtttaattgt gcggtcaaaa atgttgcttt cgaaaaaaat
      841 tactatgacg taaccaataa agaaaataca aaatattggg aagagcattt tgcaacacac
      901 attgagccct tatacggcaa tgagctaaca aatataattt ctagcattat gctttctaat
      961 tcgaacgaga acgtgttaaa tccaagtcag aagttttctt tagcaatgat gattgtattt
     1021 caaataatca gagtaccaaa atttttgaaa ggaagatttc aaaacggaga agtgattttt
     1081 gatgaaacaa ttagggagtt gatacaacta atgcctatgg agtttatttc taaaaatata
     1141 gaggtattag aggattt
//
LOCUS       DASFSW010000274         1138 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.278, whole genome shotgun sequence.
ACCESSION   DASFSW010000274 DASFSW010000000
VERSION     DASFSW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.278"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            1..267
                     /locus_tag="U1099_002230"
                     /pseudo
     CDS             1..267
                     /locus_tag="U1099_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428216.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
     gene            789..>1138
                     /locus_tag="U1099_002231"
     CDS             789..>1138
                     /locus_tag="U1099_002231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868494.1"
                     /translation="MIALLERYQWFSKTSYNSLYGNVDFSNPLERYVELFVAHSRKFG
                     WLVLTPVFLYRIFFSGGENLYRNDLIRTVSILSFPIIGLVGLYFVIALGASQIKGYYI
                     SKYMEDYRILSGYS"
BASE COUNT          353 a          140 c          216 g          429 t
ORIGIN      
        1 tagaagtgaa aatgtagact taattattat caataggggt tttattgttc ttcaggatgg
       61 aaaagaagtt tattttgatt atgggcagtt cgaatcccac agggattggt tactccagag
      121 gaaacttact ttttcaatca agaaaacatt gtagctatag tatttcgtgg ttatgtcaac
      181 attgatgagc aatctttttc aagtcagtgt gatacattga tttcagaatc tggttatgaa
      241 aaaggaaaag ttctatcata taattagtaa tttgaatcct cagtaaattg tttgctgggg
      301 attatttatt gtataatagg aaagtatgat aatctgataa ggatgagtaa acataaaaaa
      361 acagtatgga atgcctcttt agaagagggc acggggttgg taacggatcg gtacattcag
      421 acttcaatgg aggacataga gaaaaatggt tatggaaaga aaataatagg ttttttgact
      481 gttaatctct tcatgtattt aataacattt ataggtagat atgttgattc acaaatcata
      541 ttaactctat ttaaaagtac ggaggtatta gtaagtattc ctttctttct agggctactt
      601 gttacacttt tgtatttcat ttccttgaaa aaatccatca aaaacgagtc ttgtcttact
      661 attattttaa taagtatatg tttttgctta catttctatt ctattgccaa atagaaatat
      721 tactagttat tttttcgaca atgatacttg gtgagttctt gtcaatattc atctattcgg
      781 tggttttcat gattgccctt cttgaaagat atcaatggtt tagtaagact tcgtacaata
      841 gtttgtatgg taatgtagat ttcagtaacc cactagaaag atatgtggag ctttttgttg
      901 ctcattctag aaaatttggt tggctcgttc ttactccagt ttttctgtat aggatatttt
      961 tttccggcgg agaaaatttg tatagaaatg atttaataag aacggttagc atcctttcgt
     1021 ttcctattat tggattggtt ggtttatatt ttgttattgc acttggtgca agtcagataa
     1081 aaggctatta tatcagtaaa tatatggaag attatcgtat tctctcagga tatagcat
//
LOCUS       DASFSW010000275         1124 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.279, whole genome shotgun sequence.
ACCESSION   DASFSW010000275 DASFSW010000000
VERSION     DASFSW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.279"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            13..978
                     /locus_tag="U1099_002232"
     CDS             13..978
                     /locus_tag="U1099_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM4868495.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLVAPPKYTYKKEINRKVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKAYFT
                     MALDFISERVGEQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKTILGNQKSLSEWQ
                     TAYHERMSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYGEIVSALSDINAFNA
                     GKKRDKALELVAAWLPEVEKFSKEIGRQQAYIDSLKEQIGQEADYAGRMRDEKYEQEL
                     KVQKANQRIFELQRTNEQMGRLLSKIPPEVLEELQRTGRNKSRER"
     gene            986..>1124
                     /locus_tag="U1099_002233"
     CDS             986..>1124
                     /locus_tag="U1099_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEM4868496.1"
                     /translation="MKKQDFKVLKTKDLYPFPDNPFHVVEDETLSELAESIKEFGIVT
                     PI"
BASE COUNT          359 a          248 c          301 g          216 t
ORIGIN      
        1 agatgatgga ttatgcccta tgcaatcctg cgtttccaga aacgcaaagc gggcggcgtt
       61 gcggcttgcg aacgccacaa cgagcggaag aaagaagcct acaaaagcaa cccagatatc
      121 gacatggaac gctctaaaaa taactatcat cttgtcgcac cgccaaagta cacctataag
      181 aaagagatta accgaaaggt agccgaagca gggtgcagga caaggaaaga cagcgtgatg
      241 atggtggaaa cgctcatcac ggcttcacca gaatttatga accagttacc gcccgaagaa
      301 cagaaagcgt attttacgat ggctctggat ttcatttcgg agcgtgtcgg ggagcagaat
      361 atcctctccg cagtcgtcca catggacgag agaacgcccc atatgcacct ctgctttgtg
      421 ccgattacac cagacaataa gctgtccgcc aaaacaattt taggcaatca aaagagcctg
      481 tcggagtggc agaccgccta ccatgagcgg atgtcctcac gttggaatca gcttgagaga
      541 ggtcaatctt cgatggaaac caagcggaaa cacgtcccca catggctcta taagttgggc
      601 ggcaggcttg ataagcagta tggggaaatc gtgtctgccc tgtccgacat caacgccttt
      661 aacgcaggga aaaagcgtga caaggctctg gaactggttg ccgcatggct gcccgaagtg
      721 gagaaattct ctaaggaaat cggcagacag caggcgtata tcgacagctt aaaggagcaa
      781 atcgggcagg aagccgacta tgcagggcgt atgcgtgatg aaaagtacga gcaggaatta
      841 aaggtgcaga aagccaacca gaggatattt gaattgcaga gaaccaacga gcagatgggg
      901 cggctgctct caaagatacc gccagaagtg ttggaagaat tacagagaac aggcagaaac
      961 aaatcaagag aaaggtaaaa agtgaatgaa gaaacaggat tttaaggtgt taaagaccaa
     1021 agatttatac ccgtttcccg acaatccgtt tcatgttgtg gaggatgaaa cgctgtcaga
     1081 gttagcggaa agcatcaagg aatttggcat tgtcacgccg ataa
//
LOCUS       DASFSW010000276         1076 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.280, whole genome shotgun sequence.
ACCESSION   DASFSW010000276 DASFSW010000000
VERSION     DASFSW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.280"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            36..1064
                     /locus_tag="U1099_002234"
     CDS             36..1064
                     /locus_tag="U1099_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM4868497.1"
                     /translation="MIKFVKNKYFNWIISLITLIILVACSSNQTLIRDNSANIDKIRI
                     AYMPNFASLHDIITGINSGIFKEEGLEVELIEFADGPTIIASLESGSIDIGNIGPGAH
                     KLAIEGRAEVVAFSQLGNADELIARSDRGIDSIKDLRGKKIGTASGTSAETILKLALL
                     DANLSEKDVEIIDMDASAIVTAMLSGSIDVAATWSPNTTLIKRELKEKVVVLADNSRY
                     RDQSPAIASYVTFPGYSEKNNEKISKFLKGLYRAMDYRAEHMKEVSKWVATQIGSDIT
                     AVQDQLQDGEWLTSRQMIQLINTGILQQYYEKQQKNLIVSGIVDISRNINEYVLIDQM
                     SSILKEIK"
BASE COUNT          379 a          139 c          219 g          339 t
ORIGIN      
        1 agtgacacac ttatactctt ttttgagagg gaaaaatgat taaatttgtt aagaataagt
       61 atttcaattg gattattagt ctaatcactt taattatttt ggttgcatgt agttctaatc
      121 aaactttaat aagagacaat tcagccaata tagataaaat aagaattgca tatatgccaa
      181 attttgcaag tcttcatgac attattactg gaataaattc aggtatcttt aaagaggagg
      241 ggcttgaagt cgaattgatt gaatttgctg atggtccaac tatcattgca tctttagagt
      301 caggttcaat tgatattggt aatattggtc cgggtgccca taaattggcg attgaaggta
      361 gagcagaagt tgttgcattt agtcaacttg ggaatgctga cgaattgatt gctcggtcgg
      421 atagaggaat tgattcaata aaggatttga ggggaaagaa aattggaaca gcatctggga
      481 catcagcaga aacaattcta aagcttgctt tgttagatgc aaatttgtct gaaaaagatg
      541 tagaaattat tgatatggat gcctccgcta ttgttacagc gatgttgtcg ggatcaatag
      601 atgtagctgc aacttggtca cctaatacaa ctttaataaa gcgagaatta aaggaaaaag
      661 ttgttgtatt agctgataat tcacgttata gagaccaatc tccagctatt gcttcatatg
      721 taacttttcc ggggtattct gaaaaaaaca atgaaaaaat ttccaagttt ttaaaagggt
      781 tatatcgtgc aatggattat cgagctgaac atatgaagga agtttcaaaa tgggttgcta
      841 cccaaatcgg ttcagatatc acagctgttc aagatcaact acaagatgga gagtggttaa
      901 caagtagaca aatgattcaa ttgattaaca ctgggatact tcaacagtat tacgagaaac
      961 aacaaaaaaa tttaatagta tctggaatag tagatatctc taggaacatt aatgaatatg
     1021 ttctaattga tcaaatgagt agtattttaa aggagattaa ataattcttg tgaaat
//
LOCUS       DASFSW010000277         1063 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.281, whole genome shotgun sequence.
ACCESSION   DASFSW010000277 DASFSW010000000
VERSION     DASFSW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.281"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            7..213
                     /locus_tag="U1099_002235"
     CDS             7..213
                     /locus_tag="U1099_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM4868498.1"
                     /translation="MESQDKVIESLTKTIEIMTNELTLLREQVEYLRQKLYGKSSEKV
                     VYQPGQLSLFGEESLPEEEADLPS"
     gene            167..919
                     /locus_tag="U1099_002236"
     CDS             167..919
                     /locus_tag="U1099_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM4868499.1"
                     /translation="MGRKASLKKKLTYPVETETITYKRKKAKGVRQAIFSQFTPEIVH
                     HELQGKDCTCPDCHGQLKEIGSTVQRQELVFIPAQLKRIGHVQHAYKCQACSQKNLSD
                     KIIKAPVPKAPLAHSLGSASIIAHTIHQKFNLKIPNYRQEEDWHKLGLPISRKEIANW
                     HIKSSQYYFEPIYNLLHEKLLEQPILHADGTSYKVLENDSQLTFYWTFLSGKHEKKGI
                     TLYHHDKRRSGLVVEEFLGDYAGYVHCDMLRQ"
BASE COUNT          309 a          225 c          253 g          276 t
ORIGIN      
        1 aagtccatgg agtcacaaga taaagtgatt gaaagtctaa caaaaactat tgaaatcatg
       61 accaacgagc taaccctcct tcgtgaacag gttgagtatc tgagacagaa actttacgga
      121 aaatcatcag agaaggtcgt gtatcaacct ggtcagttga gcttgtttgg ggaggaaagc
      181 ctccctgaag aagaagctga cttacccagt tgaaacagaa acgattactt ataaacgcaa
      241 gaaagctaag ggagttcgtc aggctatttt tagtcaattc actccagaga ttgttcatca
      301 cgaattgcag ggcaaagact gcacttgtcc agactgtcat ggtcagttga aggagattgg
      361 ttctactgtt caacgacaag agttggtctt cattcccgca caattaaagc ggattggcca
      421 tgttcaacac gcatacaagt gtcaggcatg tagtcagaag aatctaagcg ataagattat
      481 caaggctccc gttcctaagg cacctttggc acatagcctg ggttcggctt ccatcatcgc
      541 ccacaccatt caccagaaat tcaatctgaa gatacccaat taccgtcagg aagaggactg
      601 gcataagctt ggtctgccca tcagtcggaa ggaaatagct aactggcaca tcaagtctag
      661 tcagtattat ttcgagccga tttataacct gttgcacgag aaattgttgg agcaacctat
      721 tctccatgcg gatgggacct cctacaaggt cttagaaaat gatagccagt tgaccttcta
      781 ctggaccttc ttgtctggga aacatgagaa aaagggaatc acgctctatc atcacgacaa
      841 gcgtcggagt ggcttggttg tggaggaatt tcttggggac tatgcgggct atgtacattg
      901 tgatatgtta cggcagtaac ttaggacttt agtcctttaa ttctgcctat gcgatagcag
      961 tccaaggttt aggagccaag cgacgttaag cttggtgaac tgctaaccgc ttaagtgctt
     1021 atcgtcaatt agacaaggct cagctggttg gttgttgggc tca
//
LOCUS       DASFSW010000278         1030 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.282, whole genome shotgun sequence.
ACCESSION   DASFSW010000278 DASFSW010000000
VERSION     DASFSW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.282"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>1030)
                     /locus_tag="U1099_002237"
     CDS             complement(<1..>1030)
                     /locus_tag="U1099_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM4868500.1"
                     /translation="SFLLYTHLIQNELGSTKNDGTVEIIAEQPNQVIDSIQINGRYIH
                     SSEIIENQWGINDADLIPNFSSLGEENSLVIKSSSSVRTLSFEYFVSENPTIVKIKVG
                     GQLVESIDTSTGDKYKNLAFIELPYTLRITKDNQFWYLHLIVLALGLTCFILNGATWR
                     IKRRDIHILTILLIVQYIFTTFTFPRLYRNELVLFNSNFNKMETQQLLVALTFLIFFG
                     LLGYKQLRGHISKAFKTISLSVIYLLVPIFSLFIIENSYSQFSTLSSNSLWNNLIIIG
                     VLYLILFFTTNLRFASLLILSASVFIGISNQLLIDSRGAPLLFYNLFQIIDGLNVASS
                     VAININNRM"
BASE COUNT          357 a          137 c          190 g          346 t
ORIGIN      
        1 cattcgattg ttgatattaa ttgctacgct agaagctaca tttaatccgt ctatgatttg
       61 aaaaagatta taaaataata gaggagcacc acggctgtca atcaaaagtt ggttacttat
      121 accaataaat actgaagcgg atagaattaa taggctggca aatcgtaagt tcgttgtaaa
      181 gaacagtatt aggtagagga ctccgataat gattagattg ttccaaagac tgtttgagga
      241 aagagtagag aattgtgagt agctattttc aataataaat aatgaaaaaa tgggaactag
      301 aagataaatc actgatagac taatcgtttt gaaggcttta gagatatgac ctcggagttg
      361 tttgtatccc aaaagaccaa aaaatatcag aaaagttagt gcgactaata gttgttgagt
      421 ttccatttta ttaaagttcg aattaaatag cactaattca ttgcgatata aacgtggaaa
      481 tgtaaaagta gtgaatatat attgaacgat taataatatt gttaagatat ggatatctct
      541 tcttttgatt ctccatgttg ctccatttaa gataaagcat gttagaccta aagcaaggac
      601 tattaagtgg aggtaccaaa attgattatc tttagttatc cgtaaagtat agggaagctc
      661 aataaaggct aaatttttat atttatctcc agttgatgtg tcaatggatt caaccaattg
      721 tccaccgact ttaattttta caatagtcgg attttcacta acaaagtatt caaatgataa
      781 ggttcttaca gaagaggaag atttaattac gagtgaattt tcctcaccta gagaagagaa
      841 attagggatt aaatcagcat cattgattcc ccattggttt tctataattt ctgatgaatg
      901 aatatagcgt ccatttattt ggatgctgtc gattacttgg tttggttgtt ctgctataat
      961 ttctacagtt ccatcatttt tcgtagaacc taattcgttt tgtattagat gtgtatacag
     1021 taaaaaactt
//
LOCUS       DASFSW010000279         1027 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.283, whole genome shotgun sequence.
ACCESSION   DASFSW010000279 DASFSW010000000
VERSION     DASFSW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.283"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..963
                     /locus_tag="U1099_002238"
     CDS             <1..963
                     /locus_tag="U1099_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM4868501.1"
                     /translation="QTLVTFYPFILLLIIVLAVSVAFVYSRLSTRRIARISETTRRMQ
                     SLESGISCAVAGTDEITILAQDINSLYSKLLSSIDELRTENERAMAREKEKSDFLRIT
                     SHELKTPIASMLGLVEGMIYNVGPFKDHDTYLKKCKEILQEQSELVHSILEATNLDMA
                     LKESREQIRLDQLIDSSLTSYQSLAEVKGYQFEVELSPVLIEANPVYFLKAIKNILDN
                     AFRYSCKEAIIRVVLRQNQLIIENQVERVLSDEELDQVFRPFYRPDYSRDKKDGGIGI
                     GLFIVQKILEKHGFPYTFYASDTQTMRFMIHLQSQSTANSNELI"
BASE COUNT          336 a          158 c          207 g          326 t
ORIGIN      
        1 caaacattgg taacttttta tccattcata ttgctgttga ttattgttct ggcagtttcg
       61 gtagcttttg tttacagtag actgtccact cgtagaatag caagaatttc agaaacaaca
      121 agacgaatgc aatcgttaga gtctggaatc agttgtgctg ttgcaggaac tgatgaaata
      181 acaatattgg ctcaggatat taacagcctg tatagcaagt tattgtcaag tatcgatgaa
      241 ttaagaacgg aaaatgaacg tgctatggca cgtgagaagg agaagtctga tttcctccgt
      301 atcacatcac acgaattaaa aactccaata gctagtatgt tggggttagt cgaaggcatg
      361 atttataatg tggggccatt taaggaccat gatacatatt taaaaaaatg caaggaaatc
      421 ttgcaggaac aatctgagtt agttcattct attttagaag cgacaaatct tgatatggct
      481 ttaaaggaaa gcagagaaca aatccgatta gatcagttga ttgattcctc tttaacaagt
      541 tatcagagtt tggcagaggt taagggctat caatttgaag tagaactatc accggttctc
      601 attgaggcaa atcctgttta tttcttaaaa gctattaaga atattttaga taatgccttt
      661 cggtactcat gtaaagaggc tattattcgt gtagtactac gccaaaatca attaataatc
      721 gaaaatcagg ttgaacgtgt tttaagtgac gaggaattgg atcaagtctt tcgtccattc
      781 tatcgtccag actacagtcg tgataagaaa gatggtggca tagggattgg tttgtttatt
      841 gtacagaaaa tattagaaaa acacggcttt ccgtacactt tttatgcttc tgatacacaa
      901 actatgcgtt ttatgattca tttgcaatca caaagtacag cgaatagtaa tgaattgata
      961 taggtaaaat atagacaata tttagttttt cttttattac ttcgacgtaa gaggaaactc
     1021 cgtttcc
//
LOCUS       DASFSW010000280         1012 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.284, whole genome shotgun sequence.
ACCESSION   DASFSW010000280 DASFSW010000000
VERSION     DASFSW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.284"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..863)
                     /locus_tag="U1099_002239"
     CDS             complement(<1..863)
                     /locus_tag="U1099_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM4868502.1"
                     /translation="MMKRYSINKRILRTVLEIVIGTVANVIMLLVLSYVLIFTGQLNT
                     YGTDYHISISDSKSANGIKNQMDYSVFDYVLFDSDNGELVSGNYQKQDLQHYKEVFDT
                     GSSVSEGSILFTKYENSDFILVIRRPTMPEFVNRNFRHISYNLVSYLFFIVLELLLIS
                     ISVIRLLREFTKNFRLIEKISLNMGIPGMDIGRKETAILECNNILLTLYQKDEELTKL
                     LEMEKKDKEDLSFQVAALAHDIKTPLTVLKGNIELLEMTNLDEKQLDFMSSMNHSVGI
                     FEKYFNSMVNYS"
     gene            complement(841..>1012)
                     /locus_tag="U1099_002240"
     CDS             complement(841..>1012)
                     /locus_tag="U1099_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM4868503.1"
                     /translation="REEIYECVYGDEADALFRSISEYIYQIRVKFGPYGINPIKTIRG
                     MGYKWNDEKVFN"
BASE COUNT          352 a          187 c          118 g          355 t
ORIGIN      
        1 gagtaattaa ccattgaatt aaaatacttt tcaaatatac caacactatg attcattgag
       61 gacataaaat ctaattgttt ttcatctaga ttagtcatct caagcaactc gatattcccc
      121 tttaaaacag ttaatggtgt tttaatatca tgtgctaatg cagcaacttg aaatgacaaa
      181 tcttctttat cttttttctc catttctaat aactttgtca actcttcgtc tttttgataa
      241 agcgtcaata aaatgttatt acattctaag attgcagttt ctttacgtcc aatatccatt
      301 cctggtattc ccatattcag agatattttt tcaattagac gaaaattttt tgtaaattct
      361 cttaagagcc taataacaga gatagagatg agtaataatt ctagcactat aaaaaacaaa
      421 taagagacta aattatagga aatatgtcga aagtttcgat ttacaaattc gggcattgtt
      481 ggtcttctaa taaccaaaat aaaatctgag ttttcatatt ttgtaaacaa tatagaaccc
      541 tcgctaaccg aacttccagt atcaaaaact tctttataat gttgtaaatc ctgtttttga
      601 taatttccag agaccaattc gccattatcg ctatcaaaca atacataatc aaagacagaa
      661 taatccattt gattcttaat accgttagca gatttactat cactaattga aatatgatag
      721 tctgttccat atgtgttcaa ctgacctgta aaaataagaa cgtagctcaa tacaagcaac
      781 ataataacat ttgcaactgt accaatcact atttcaagaa ctgtccttaa aatccgctta
      841 ttaattgaat accttttcat cattccactt atatcccatc ccccttattg ttttaatggg
      901 attaattcca tagggaccaa acttgactct aatttgataa atgtattccg aaattgaacg
      961 aaataatgca tctgcttcat caccatagac acattcatag atttcttctc tt
//
LOCUS       DASFSW010000281         1010 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.285, whole genome shotgun sequence.
ACCESSION   DASFSW010000281 DASFSW010000000
VERSION     DASFSW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.285"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>1010
                     /locus_tag="U1099_002241"
     CDS             <1..>1010
                     /locus_tag="U1099_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEM4868504.1"
                     /translation="SDGKMNLHAPGISSIKPILTKPIEDNATSGYEIGFTLQQIGEVK
                     PWNTFRIVFKEDHDGFIYALEFSGQAAFLKKLSSIDAPLQSGEKYAETSHILGSEEHK
                     VRLVVKGNSVTVSDNGIPLLSYNSPNNWNDAQASIVFSPVSNRSVSLDDIIVRRTQTL
                     RPFRVVSRLDGQEVSGVLPGSIKGTTSQVFVGDSLNLEVIEKSGYQFIGFKDSAGNFL
                     DLTTFVVPNEESELVLYADFESVDVVIREPKTFYIDSLGGNDSNNGETENSAWKSFEQ
                     LRKKSFVAGDRILIKRGSRFVGESAFLTFKGSGSQNTPILISSYGEGELPLLEGQGKI
                     EN"
BASE COUNT          334 a          143 c          212 g          321 t
ORIGIN      
        1 tagtgatggg aaaatgaatc ttcacgctcc aggtatctct tcgatcaaac caattttaac
       61 aaaaccaatc gaagataatg caacttctgg atatgaaatt gggtttacat tacaacaaat
      121 tggtgaagta aagccatgga atacttttag aatagtattt aaagaagatc atgatgggtt
      181 catttatgct ttagaatttt caggacaagc tgctttttta aaaaaacttt ctagtataga
      241 tgcaccactt caatctggcg aaaaatatgc tgaaacaagt catattttag gaagtgaaga
      301 gcataaagta cgacttgtag tcaaaggtaa ttcagtaaca gttagtgata atggaatacc
      361 attattaagt tacaacagcc caaacaattg gaatgatgcc caagcatcta ttgttttttc
      421 accagtttca aatcggagtg tttctctaga tgatattatt gttcgaagaa ctcaaacctt
      481 aagacctttc cgggtcgttt ctcgattaga tggtcaggag gtatctggag ttctgcctgg
      541 ttcaattaaa ggaaccacaa gtcaagtttt tgttggagat tcacttaatt tagaggtcat
      601 tgaaaaatct gggtaccaat ttattggttt taaagatagt gctggtaatt ttcttgattt
      661 aactactttc gttgttccaa atgaggagtc agagttagtg ctgtatgccg attttgagtc
      721 agtggatgta gtgattcgtg aaccaaagac tttctacatt gatagtctag gaggaaatga
      781 ttctaataac ggggaaaccg aaaatagtgc atggaaatca tttgaacaat taaggaaaaa
      841 gtcgtttgta gctggagata gaatattaat aaaacgtgga agtcggtttg ttggagaaag
      901 tgcttttctt acatttaaag gttcgggtag tcaaaatact ccaattctaa tttcatccta
      961 tggtgaaggt gagttacctt tattagaagg gcaagggaaa attgaaaatg
//
LOCUS       DASFSW010000282         1009 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.286, whole genome shotgun sequence.
ACCESSION   DASFSW010000282 DASFSW010000000
VERSION     DASFSW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.286"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>1009
                     /locus_tag="U1099_002242"
     CDS             <1..>1009
                     /locus_tag="U1099_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM4868505.1"
                     /translation="DIDYRIGSRWIPLAVYGKFAQETFMGQTFDLTDQEVSTVLEVSP
                     IDGTMSYQSKFAFRYSTATDRSLGVPGSRYDSGRKIFENLLNSNQPTITKQVEDGDKK
                     KHVTDVEKTTVLRAKETELQELFQDFVASYPEVQQMIEETYNSLYNRTVSKVYDGSHL
                     TIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKP
                     LYVVPSSLTAQFGQEIMKFFPTKNVYVTTKKDFAKAKRKQFVSRIITGDYDAIVIGDS
                     QFEKIPMSHEKQVTYIQDKLQQLRDIKQGSDSDYTVKEAERSIKGLEHQLEELQKLER
                     DT"
BASE COUNT          318 a          202 c          226 g          263 t
ORIGIN      
        1 tgatattgat tatcggattg gctcacgttg gattcctcta gccgtttatg ggaaatttgc
       61 ccaagaaacc tttatgggac aaacttttga cctgactgac caagaagtat caactgttct
      121 tgaagtcagt ccaattgatg gcacaatgtc ctaccaatcc aagtttgcct ttaggtattc
      181 taccgcaacg gatagaagtt taggtgtgcc tgggtcacgc tatgatagtg gccgaaaaat
      241 ctttgaaaac ctgcttaatt ccaatcaacc aaccattacc aaacaagtgg aagatgggga
      301 taagaaaaag catgtgacgg acgtcgagaa gacaacggtt cttcgtgcta aggaaactga
      361 gcttcaagaa ctcttccaag actttgtggc aagttaccca gaggtgcaac agatgattga
      421 agaaacctat aatagccttt ataatcgtac agtttcaaaa gtctatgatg gcagtcattt
      481 aaccattgat gggcttgccc agaatatttc cttacgccct caccagaaga atgccattca
      541 acggattgtg gaagaaaaac gagcgcttct agctcatgaa gtgggttcag gtaagacttt
      601 aaccatgctt ggggcaggct tcaaactgaa agaactcgga atggtgcata aacctcttta
      661 tgtggtgccg tcaagtctga ccgctcagtt tggccaagaa attatgaagt tcttcccaac
      721 gaagaatgtc tatgtgacaa ccaagaaaga ttttgcgaaa gccaaacgta agcagtttgt
      781 ctcacggatt atcacagggg actacgatgc catcgtcatt ggggattccc agtttgagaa
      841 aattcccatg agtcatgaaa agcaagtgac ctatattcaa gataagttac aacaacttcg
      901 tgacatcaag caaggtagtg atagtgatta tactgttaaa gaagcagagc gttctattaa
      961 gggcttagag caccaattgg aagaacttca aaagttggaa cgtgatacc
//
LOCUS       DASFSW010000283         1007 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.287, whole genome shotgun sequence.
ACCESSION   DASFSW010000283 DASFSW010000000
VERSION     DASFSW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.287"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            140..952
                     /locus_tag="U1099_002243"
     CDS             140..952
                     /locus_tag="U1099_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM4868506.1"
                     /translation="MMKKYIMTGLVLLATIGLSACSGNATQEDTSLKDVQEKGKLIVA
                     LNPEFPPFEFRTLVDGKDTIVGADIELAKAIGQELGVEVEFSAMSFDNVLNNVQSGQS
                     DIAISGISATEERAKVYDFSIPYYTSTNKVIINKEDLAQYTSLDSLAEANIGAQKGSI
                     QEQIIKEQLPSSTVIALASNGELINQLKSQKVEAVIFEEPIAKAYVAKNDDLVILDTE
                     IKSSYSDAYAIALPKGSTALKEKVDSVITKLVESGQMDQFVQEAYELSIANN"
BASE COUNT          371 a          146 c          190 g          300 t
ORIGIN      
        1 acaaatgtat aaaaatgaca taaaatgttt ttatctttca aaaaaggaga aaaaatatgc
       61 attttactac aatgaaagtc cgatgtttta tataaaaaac ggtataagta tgcacaggat
      121 tattttaaag gagaatatga tgatgaaaaa atatattatg acaggcttgg ttttactggc
      181 aacaataggt ctaagtgctt gtagtggaaa tgctactcaa gaagatactt ccttgaagga
      241 tgttcaggaa aagggaaaac taattgttgc attaaatcca gaatttccac catttgagtt
      301 tagaacactg gttgatggaa aagatacaat cgttggagct gacattgagc ttgcaaaagc
      361 cattggtcaa gaattgggtg tagaagtcga attttcagcg atgagttttg ataatgtttt
      421 gaacaatgtc cagtcagggc aatcggatat tgccatttca ggaatttcag ccactgaaga
      481 gcgtgcaaaa gtgtatgatt tttccattcc ttactacacc tcaaccaata aagttattat
      541 aaataaagag gatcttgcac aatatacatc gcttgattct ctagctgagg caaatatagg
      601 agcacaaaaa ggttctattc aagaacaaat tattaaagaa cagcttccat cctccacggt
      661 tattgctcta gcttcgaacg gagagctgat taatcaatta aaatcacaga aggtagaagc
      721 agttattttt gaagagccga ttgcgaaagc ctatgttgcc aaaaatgatg atttagttat
      781 tttagatact gagataaaaa gtagctattc agacgcctat gctattgcct tgccaaaagg
      841 aagtacagct ttaaaggaaa aagttgactc tgtcattaca aaactagtgg aatctggaca
      901 gatggatcaa tttgttcaag aagcctatga attatccatt gctaacaatt aaattttata
      961 catttaaagt gaataaaaat aatattttct tgacaaatgt ataaaaa
//
LOCUS       DASFSW010000284         1005 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.288, whole genome shotgun sequence.
ACCESSION   DASFSW010000284 DASFSW010000000
VERSION     DASFSW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.288"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>1005
                     /locus_tag="U1099_002244"
     CDS             <1..>1005
                     /locus_tag="U1099_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4868507.1"
                     /translation="LYQGLFPNGLMQVTPDYFSQSYLLGDVNYQTVGLEDKGAIMETY
                     SDLINSLDDKTNFQLTIFNQRVNLDKFRKSVLYPLHEDGYDTYREELNRIMENNLEAG
                     ENNFSAVKLISFGKSDQNPKLAYRSLSQIGEYFKSGFSEIDANFTLLSGESRVNHLAD
                     MLRGENHLPFTYQDLVRSGQTTKHFIAPTSLSFKHKNHIEMDDRLLQIVYVRDYGMEL
                     GDKFLRELMQSDLEVMISLHAKGAAKSEAMTKLRTKKTLMESQKIGEQQKMARSGVYL
                     EKVSQVLESNIDEADELIKTMTQTGDKLFDTLFVIGVFAEDEDQLKHSLDIIKQVAGS
                     "
BASE COUNT          305 a          194 c          223 g          283 t
ORIGIN      
        1 ttctttacca aggccttttt cctaatggtc tcatgcaggt taccccagat tatttttccc
       61 agtcctatct tttaggggat gtcaattacc aaacggtggg acttgaagat aagggagcga
      121 ttatggaaac ctattcagac ttgattaaca gtttggatga caagaccaat ttccaactca
      181 ctatttttaa ccaaagggtc aacttggata aatttagaaa aagtgtctta tatcccttac
      241 atgaagatgg ctacgatact tatcgtgaag aactcaatcg tatcatggag aataacttag
      301 aagctggaga aaataacttt tcagcggtta agctcatttc ttttgggaag tcagaccaaa
      361 atccgaaact cgcttatcgc tccctgtctc agattgggga atatttcaag agtggttttt
      421 ctgaaataga tgcgaatttt actctcctca gtggagaaag ccgtgtgaac cacttggctg
      481 atatgttgcg aggggaaaat catttgccct ttacttatca agacctggtg cgttcaggac
      541 aaacaaccaa gcactttatt gccccaacca gtctgtcctt taagcacaaa aatcatattg
      601 aaatggatga tagactgctt cagattgtct atgttcgtga ctatgggatg gagttgggag
      661 ataagttctt acgagaactc atgcagtcgg atttagaagt gatgattagt cttcatgcca
      721 aaggagcagc caagtcagaa gccatgacca agctccgcac caagaagact ttgatggaat
      781 cgcaaaaaat tggggagcaa caaaagatgg cacgatctgg cgtttatctt gaaaaggtta
      841 gtcaggtcct agaaagtaat attgatgaag ccgatgagtt gattaaaacc atgacgcaaa
      901 caggcgacaa gctctttgac accctttttg tgattggggt ctttgcggag gacgaagacc
      961 agttgaagca ctcccttgat attatcaaac aagtggcagg ttcta
//
LOCUS       DASFSW010000285          997 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.289, whole genome shotgun sequence.
ACCESSION   DASFSW010000285 DASFSW010000000
VERSION     DASFSW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.289"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(452..>997)
                     /locus_tag="U1099_002245"
     CDS             complement(452..>997)
                     /locus_tag="U1099_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4868508.1"
                     /translation="IKYNELSRETVAERVMKTGREMAVRAYNVERKQKEKKFLKPIIF
                     VLDGFKGDEYIHPNPSNHLTILTEMYNVRGLLTDNHQIKFLKVNYRLIITPDFAKFLP
                     HEFIVVPDTLDIEQVKSQYVGTGVDLSKIISLKEYRKEIGFIGNLYALLGFVPNMLNR
                     IYLYIQRNGIANTIIKIKSRL"
BASE COUNT          358 a          169 c          118 g          352 t
ORIGIN      
        1 agttataaaa agagcgaata acaaatgatt tgaaatccgt ctaaaatcat caatatcttt
       61 tatcaaactt attggcaacg ttgctaaaac tgaaataatc atcattgaaa ttgataaata
      121 tattttagtt gttataggaa gaatttgctg acatattccg atagtaaata aaaatataat
      181 aagaaacaaa taccctaatt tataaactac aatatgtttg ttatttttta ataaatcatt
      241 aactatacat aaaatcaaaa ctaaataaga tagatactga atcacagaag ctttgcctat
      301 cgctagaaca acttgcgaat tactaataat tatgcccata aacgctatgg caacaaaaaa
      361 caaattatta attcttattc tcatttttcc aaacgttttt ctcctatctg cctgcattac
      421 ttttaaaatt acaaataaaa gtaaacaact ctcacaatct cgacttgatt tttataatag
      481 tgtttgcaat accgtttctc tgaatatata gataaattct attgagcata ttcggaacaa
      541 atcctaaaag cgcatacaaa ttaccaataa agcctatctc ttttcgatac tcttttaaag
      601 aaataatctt tgacaagtct acacctgtac caacatactg gcttttaact tgctctatat
      661 ccaaggtatc tggtacaaca ataaattcat gcggtaaaaa cttagcaaaa tctggagtta
      721 taattaatct ataattaact ttgagaaatt taatttggtg attatcggta agtaagcctc
      781 tgacgttata catttcggtt aagatcgtca aatgatttga tggattggga tgaatatact
      841 catctccttt aaacccatct aatacaaata taataggttt tagaaatttc ttttcttttt
      901 gttttcgctc cacattatat gcacgaacag ccatttctct tcccgttttc atcacgcgtt
      961 ctgctactgt ttctctggat agttcgttat attttat
//
LOCUS       DASFSW010000286          996 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.290, whole genome shotgun sequence.
ACCESSION   DASFSW010000286 DASFSW010000000
VERSION     DASFSW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.290"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            274..663
                     /locus_tag="U1099_002246"
     CDS             274..663
                     /locus_tag="U1099_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868509.1"
                     /translation="MKDNNRETIIHFEMSNQEYAPVKNAISEELKTVISKVYQLDHET
                     GWTLHYLTEIMLNHFHEDVARVQYGDLTPIECSLNSIASRVEKVRIELLKAGYEDKIE
                     VGNPMWYLKLALQDFEQMKKEMKKCKK"
     gene            651..854
                     /locus_tag="U1099_002247"
     CDS             651..854
                     /locus_tag="U1099_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868510.1"
                     /translation="MQEMTIETALTLIAIFTPLNLYLWFGVGLGTFRLDIEPKIKTEG
                     KYTKPIENERYGAYIQLAGKRYN"
BASE COUNT          367 a          194 c          214 g          221 t
ORIGIN      
        1 cccttaaaac tggagcagaa aagtattagg tgctggtatc gccattagcg aaagctaaac
       61 caccctaagc aaactacaca cagcaaggct attattttgg cacaggctta cacgaccaca
      121 gggcaacctg gtaagtctgg ggcggttatc cgctgggaat agtctaggct aggtatagaa
      181 atcgtatagt aaagaaacca agccttttac acacgggaaa agcccactag ggccattaca
      241 cagacacaat aaaagaaacg aggcaagaac tacatgaaag acaataacag agaaacgata
      301 atccattttg aaatgtctaa ccaagaatac gcacccgtaa agaatgcaat atcggaagag
      361 ctgaagacag tcatcagcaa agtgtaccaa ctagaccatg aaacagggtg gacattgcac
      421 tatctaaccg aaatcatgct gaaccacttt catgaggatg ttgcacgagt tcaatatggc
      481 gatttgacac cgattgagtg cagtttgaac agtatagcct cacgagttga aaaagttaga
      541 atagaactct taaaagctgg atatgaagac aagattgaag taggtaatcc tatgtggtac
      601 ttgaaactag ccttacaaga ttttgagcaa atgaaaaagg agatgaaaaa atgcaagaaa
      661 tgacaatcga aacagcttta actttgatag caatctttac accgctgaac ctctatctat
      721 ggtttggcgt tggtttgggc acttttcggc ttgatataga gcctaaaatc aagaccgagg
      781 gtaaatatac caaacctatt gaaaacgagc gctacggggc ttatatacag cttgcaggca
      841 aacgctataa ctaggagggg aaaccatgct gacatttaga gaacttgaac atatagcaga
      901 gaccattctc aaacacacaa caccagaaga aatgcagtgc tatcttgata tggaacacga
      961 tagtaaattg ctttggataa aatacaaaat cgcaag
//
LOCUS       DASFSW010000287          983 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.291, whole genome shotgun sequence.
ACCESSION   DASFSW010000287 DASFSW010000000
VERSION     DASFSW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.291"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(24..758)
                     /locus_tag="U1099_002248"
     CDS             complement(24..758)
                     /locus_tag="U1099_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
                     /protein_id="HEM4868511.1"
                     /translation="MKSYNAYQLKLAMAGLMVLDHLKIIPDFIPDQLALVFHLATRCV
                     AVFFAYMLVEGFLHTRNVKAYLLRLYGAAGLMATGNTLLNSLYQSKGIYISNNIFLTL
                     AVGLTLLICLQNFSEHTRAKQIIQVLLAGLLTIIGALFTEGGTVVLPFILITYLGRES
                     ATKRTIAYGFLALVLLAMSYQPYERLEMTIQMMLYNADWLFILVLPILSLYNGQAGPR
                     TVFSRYFFYIFYPLHLWILATIGYFL"
     gene            complement(771..908)
                     /locus_tag="U1099_002249"
     CDS             complement(771..908)
                     /locus_tag="U1099_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868512.1"
                     /translation="MSFKFLQAKQIFGKSQESKRLEEQDKQAEFLFELEHELFQRNWM
                     Y"
BASE COUNT          306 a          195 c          202 g          280 t
ORIGIN      
        1 aaaaaactta caagagtgta agtttagaga aaataaccaa tcgtggccag tatccacaaa
       61 tgaagcggat agaagatgta aaagaaatag cgtgaaaata ctgtgcgggg ccctgcttgt
      121 ccattataaa gactaagaat tggaaggact aaaataaata gccagtctgc attgtagagc
      181 atcatctgaa tggtcatctc aagcctttcg tagggctggt aggacatggc taataaaact
      241 agagccagaa aaccataggc aatggttcgc ttagtagcag actcacgacc caaataggtg
      301 atgaggatga atggcaagac caccgtgccc ccttctgtga acaaggcacc tatgattgtc
      361 aataaccctg ccagcaggac ctgaatgatt tgcttcgctc tcgtgtgctc actaaaattt
      421 tgaagacaaa tcagcagagt taggccgact gctaaggtca aaaagatatt attgctaatg
      481 taaatccctt tagactggta gagactgttt agcaaagtat ttccagtggc cattagtcct
      541 gctgcaccat acagtcgtaa cagataggct ttgacatttc tcgtatgaag aaacccttcc
      601 accaacatgt aggcaaagaa aaccgcgacg caccgtgttg ctaaatgaaa cactaaagcg
      661 agttgatcag ggataaaatc aggtatgatt ttcagatgat ccaaaaccat caagcctgcc
      721 atagcgagtt tcagttgata agcattatat gatttcacca gtcaaacctc ctagtacatc
      781 cagttgcgtt ggaacagttc gtgttctagc tcgaatagaa attcagcttg tttgtcttgt
      841 tcttctagtc gtttgctttc ttgtgattta ccaaaaattt gttttgcttg tagaaatttg
      901 aatgacatct gatttccttc tttctatttc ttatgagtct attatagaaa atatgtcgct
      961 catgttctct tacagttctg att
//
LOCUS       DASFSW010000288          950 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.292, whole genome shotgun sequence.
ACCESSION   DASFSW010000288 DASFSW010000000
VERSION     DASFSW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.292"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            23..877
                     /locus_tag="U1099_002250"
     CDS             23..877
                     /locus_tag="U1099_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM4868513.1"
                     /translation="MEKEELKILEELRRILNSKNEAIVILNNYFKGGVGKSKLSTMFA
                     YLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIVHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSTTIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
BASE COUNT          361 a          140 c          161 g          288 t
ORIGIN      
        1 aaaaaaagaa tggggcgtag ttatggagaa ggaagaactc aaaatacttg aagaattaag
       61 acgtatttta aacagtaaaa atgaagcgat tgttatctta aacaattatt ttaaaggtgg
      121 tgttggaaag tcaaaattat cgactatgtt tgcttacttg acagacaaat ttaatttaaa
      181 agttttaatg atcgataagg acttacaagc aacattgaca aaagacttag caaaaacatt
      241 taaggtagaa ttgccacgtg ttaattttta tgaaggactg aaaaatggaa acttggcttc
      301 ttctattgtt catttgactg ataatttaga cttgatccct ggcacgtttg atttgatgtt
      361 actgccaaaa ttaactcgct catggacttt tgaaaatgaa agtagattgc ttgctactct
      421 tttagcacct ttaaaaagtg actatgatct cattattatt gatactgtac caacgccaag
      481 cgtttataca aataatgcaa tcgtggcaag tgattacgtc atgatccctt tacaagcaga
      541 agaagaaagt acaaacaaca ttcaaaacta tatttcctat ttgattgatt tacaagaaca
      601 gtttaaccct ggactagata tgatcggttt tgttccttat ttagttgata cggacagcac
      661 aacgataaaa tcaaacctgg aagaactgta caagcaacat aaagaagata acttggtttt
      721 ccaaaatatt atcaagcgaa gtaataaagt aagtacctgg tctaaaaacg gcattacaga
      781 acacaaaggc tatgacaaaa aagttttatc tatgtatgag aacgtatttt ttgaaatgct
      841 tgagcgaatc attcaattag aaaacgaaaa agaatagaat cacaaatcac aagtgattaa
      901 tcacaaatca cttgtgattt gtgattccta atgatacaat attactatac
//
LOCUS       DASFSW010000289          940 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.293, whole genome shotgun sequence.
ACCESSION   DASFSW010000289 DASFSW010000000
VERSION     DASFSW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.293"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            6..836
                     /locus_tag="U1099_002251"
     CDS             6..836
                     /locus_tag="U1099_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEM4868514.1"
                     /translation="MVKINANGKEITLLSTNTDYVSLTDIAKYRNPDAPADIIKNWMR
                     SRNTIEFLGAWEKINNENFKLVEFDQFRSEAGLNGFVLSPQKWIKETNAIGITSQSGR
                     YGGTFAHSDIAFEFASWLSPEFKLYIIQDYQRLKQEEAYKNQLDWQVNRYISKLNYTI
                     QTDAIKENIVPTLQPYQISFAYSSEADLINVALFGMTAKEFKNAYPDREGNQRDNATI
                     EQLLVLNNLQSLNAEMIKQGLSQSNRLTELNRIAKEQLDVLYKNNQKALDSLKRLSDK
                     "
BASE COUNT          349 a          189 c          157 g          245 t
ORIGIN      
        1 tcataatggt taaaattaac gcaaatggta aagaaataac tttactttcc acaaatactg
       61 attatgtcag tctgacagat atcgctaaat atcgaaatcc agacgctcca gccgatatta
      121 ttaaaaactg gatgaggagt agaaatacta ttgaattcct aggagcttgg gaaaaaataa
      181 ataatgaaaa ttttaaactg gtcgaattcg accagtttag aagcgaggct ggattaaatg
      241 gcttcgtact atctccgcaa aaatggataa aagagacaaa tgccattggt ataacttccc
      301 aatctggccg atacggcggt acattcgccc actctgatat agcttttgaa tttgcttcct
      361 ggctttcacc agagtttaag ctttatatca ttcaagatta tcagcgccta aaacaagaag
      421 aagcctacaa aaaccagtta gattggcagg taaaccgcta tatttcaaaa cttaattaca
      481 ccatacaaac ggacgctatc aaagaaaaca ttgtgccaac tctccagccc tatcaaattt
      541 ctttcgccta ttcctctgaa gctgatttaa taaacgtcgc tttatttggt atgactgcaa
      601 aggaattcaa gaacgcctat cctgatagag aaggtaatca gcgagataat gccactattg
      661 aacagctatt agttctaaat aacttacaaa gtcttaacgc tgaaatgatt aaacaaggac
      721 tatcacaaag caaccgacta actgaactaa acagaattgc taaagaacag ctagacgtgc
      781 tatataaaaa caatcagaag gcccttgata gcctcaaaag attgagcgat aaataagccc
      841 atataagagt catagcacca ctttaaaacg taaccatata aattatcatc ttacccgaaa
      901 caaacgaaaa tagggctatt ctcgtaagcc tacgcatgat
//
LOCUS       DASFSW010000290          935 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.294, whole genome shotgun sequence.
ACCESSION   DASFSW010000290 DASFSW010000000
VERSION     DASFSW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.294"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..668)
                     /locus_tag="U1099_002252"
     CDS             complement(<1..668)
                     /locus_tag="U1099_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEM4868515.1"
                     /translation="MNNTLPSAFVFLASEKISSDSLFEGFNVDLESTANLVKSLADYN
                     PTVWSYMSAVTKGVMQPLGVAILAVILILEFSKMAKKIANSGGAMTFEAIAPMIVSYI
                     MVAVVITNTTVIVEAILAVASHIIEGVAGVVSHGGTTYETISGLKGSGIIGKLIVGFF
                     AILIWLVRLVSVMVVNLLITIRFIQLYLMIPFAPLTIPTFLSDDWRSVGIGYLKNIMV
                     YALQG"
     gene            complement(685..927)
                     /locus_tag="U1099_002253"
     CDS             complement(685..927)
                     /locus_tag="U1099_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868516.1"
                     /translation="MNQKLTGFVYGVDASSMFAQAMALLQKGMVATGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGVFITQITI"
BASE COUNT          294 a          218 c          193 g          230 t
ORIGIN      
        1 ccttgaaggg catagaccat gatatttttc aggtagccaa tcccaacgct tcgccaatca
       61 tcgctgagga aggtcggaat cgttaaaggt gcaaaaggaa tcatgagata gagttggata
      121 aagcgaatgg taatgaggag attcaccacc atcacactca ccaaacggac caaccatatc
      181 agaatggcaa aaaatcctac aatgagtttt ccaataattc ctgacccttt aagtccagaa
      241 atggtctcat aagtcgttcc cccatgagaa acgacaccag ctacgccttc aatgatgtga
      301 gaagcaacgg ctaagatggc ttctacaata acggtggtgt ttgtaatgac cacagccacc
      361 ataatataag agacaatcat aggagcaata gcttcaaagg tcatagcacc acctgagttg
      421 gcgattttct tagccatctt agaaaactca agaatgagaa tgactgctaa aatagccact
      481 cccaagggct gcatcactcc ttttgtgaca gctgacatat aagaccacac ggttgggtta
      541 taatccgcaa gggatttgac caagttggcc gtcgattcta agtccacgtt aaagccttca
      601 aaaaggctat cgctcgaaat tttctctgac gctagaaaga caaaagctga aggtaatgtg
      661 ttgttcatgt catacttagc cctcctaaat ggtaatttgg gtgatgaaga caccggcggc
      721 acctaccata acaccaccca caatttctaa gatggcattg cggacaccag gtccaccatc
      781 tttgatattg gttgataggt ttataatacc catgacgaca agaaaggctc ctgtcgcaac
      841 cattcctttt tgtaagagcg ccatggcttg tgcaaacatg gaactagcgt ccacgccata
      901 aacaaagcct gttaattttt ggttcatacg attcc
//
LOCUS       DASFSW010000291          930 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.295, whole genome shotgun sequence.
ACCESSION   DASFSW010000291 DASFSW010000000
VERSION     DASFSW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.295"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>930
                     /locus_tag="U1099_002254"
     CDS             <1..>930
                     /locus_tag="U1099_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4868517.1"
                     /translation="EISNFSDDFSKFESFMKKSMSKFFEMQINSRGYNPDIQKLTEEK
                     SDLLEMKDIHKLIKIRGIRANREVSNKENNHSLSKTSNLFYKSNNGDDIDNATKNLLQ
                     SAITEADEALTKAYSGDGEDDGVFTSIFERVKKFGGNDSESELEIHSSLSEKDILSNN
                     TTLYYRHDDSLLPETYNGLGYLNLYGMIFEIETLMADIKNNPADINLVYIEEPESHTH
                     PQLQYVFIKNIKGLLKEHDDELKASGYTSGIQTLITTHSSHIVSDCNFDDLIYFKHDN
                     GTAISKAFNSLKEEYEDDKLGYRFVKQYLTLNS"
BASE COUNT          356 a          112 c          169 g          293 t
ORIGIN      
        1 cgaaataagc aatttttcag atgatttttc aaaatttgag tcatttatga agaagagcat
       61 gagtaaattt tttgagatgc agattaactc acggggatat aatcctgata tacagaaatt
      121 gacagaagaa aagtctgatt tacttgaaat gaaagatatt cataaactta ttaaaataag
      181 agggattaga gctaatcgag aagtatcaaa taaagaaaat aatcattcat tatctaaaac
      241 atcaaatcta ttttataaat caaataatgg tgatgatatt gacaatgcta caaaaaattt
      301 acttcaatct gcaattacag aggcagatga agctttaacc aaggcatata gtggtgatgg
      361 tgaagacgat ggggttttca cctcgatctt tgaaagagta aaaaaatttg gtggaaatga
      421 tagtgaatca gagttggaaa ttcattcatc tctttctgag aaagacattt tgagtaataa
      481 tacaacacta tactataggc atgacgatag tttattacca gaaacataca atggtttggg
      541 atatttaaat ctatatggga tgatttttga aatagagacg ttgatggcag atatcaaaaa
      601 taatcctgct gatattaatt tagtttatat tgaggagcca gagtcgcata cacatccaca
      661 attgcaatat gtttttatca agaatattaa aggtttgctg aaggaacatg atgatgaact
      721 aaaagcaagt ggatatacta gtggtattca aactttaatt acaactcatt catcacacat
      781 agtgtctgat tgcaactttg atgatttaat ttattttaag catgataatg gcacagctat
      841 atcaaaggct tttaattcat tgaaagaaga atatgaagat gataaacttg ggtacagatt
      901 tgttaagcag tatttgacac tcaatagtag
//
LOCUS       DASFSW010000292          920 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.296, whole genome shotgun sequence.
ACCESSION   DASFSW010000292 DASFSW010000000
VERSION     DASFSW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.296"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..335
                     /locus_tag="U1099_002255"
     CDS             <1..335
                     /locus_tag="U1099_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4868518.1"
                     /translation="RNRVLAFGNPISCPAVTYNLKKLNDFKFNEEMKVSLDWFAWYQI
                     AQKNGSFTFVDESLMYHRIHEESETTNSIENNIRTKEDYEMYLLFWPKFIAKFLLSYY
                     VKSQETNN"
     gene            518..>920
                     /locus_tag="U1099_002256"
     CDS             518..>920
                     /locus_tag="U1099_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEM4868519.1"
                     /translation="MIKIFGKIRYHWQPDLSWAIIYWSLTFTPIFIGMTLLLEKLQVS
                     RLFILLVSLFAVLVVLGLHRYFEIKDEHLRIASANPFAVQKIEIATIEKIEVSYLAIR
                     IFSQEFPNGQVYHMRKWTKKYFINHLAIHSCF"
BASE COUNT          297 a          144 c          162 g          317 t
ORIGIN      
        1 ggcggaatag ggttttagcc tttggtaatc caatttcttg tccagctgtg acctataatt
       61 tgaagaaact gaatgatttc aagtttaatg aagaaatgaa agtttctctt gattggtttg
      121 cttggtatca gattgcacaa aaaaatggga gctttacctt tgtagatgag agtttgatgt
      181 atcatcgtat ccatgaagaa tcggagacga caaatagcat tgaaaacaat attcgtacaa
      241 aagaagatta tgaaatgtat ttgttattct ggccaaagtt tattgccaag tttctcttga
      301 gttactatgt taagagtcaa gagacgaata actgaaatat tttagctatc gatcattgcg
      361 ttcccaccaa ttcctactca aaatatttca aaagcatttc agaagtttta caaactaact
      421 aatattgtca tgtaaatgta ataaaaaatt tcaaaagata gaggttttca taggaaaatc
      481 tctttttttg tgctagaata atagatggag gtccgatatg atcaaaattt ttggtaaaat
      541 tcgttaccat tggcaaccgg atttatcatg ggccatcata tactggtcac ttaccttcac
      601 acctattttt attggtatga cacttttatt ggagaagttg caagtttcaa gattatttat
      661 tctacttgtc tcactctttg ctgtattagt agtgctcggc ttacatcgct atttcgagat
      721 aaaagatgag catttacgga ttgcttcggc taatcctttt gcagtacaga aaattgaaat
      781 tgctaccata gaaaaaattg aagtttctta tctagccatt cgcatatttt cacaagaatt
      841 tcccaatggg caggtgtatc acatgcgtaa atggacaaaa aaatacttta ttaaccattt
      901 agctattcat agttgcttca
//
LOCUS       DASFSW010000293          895 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.297, whole genome shotgun sequence.
ACCESSION   DASFSW010000293 DASFSW010000000
VERSION     DASFSW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.297"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(314..550)
                     /locus_tag="U1099_002257"
     CDS             complement(314..550)
                     /locus_tag="U1099_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868520.1"
                     /translation="MKIYKFMFNITYPALILCLYIIFQMKGEMSPWFDTLFLSGGFLV
                     IPLLLILLDYLSVENDKGGDNEDNQAESNGTGKV"
     gene            complement(610..>895)
                     /locus_tag="U1099_002258"
     CDS             complement(610..>895)
                     /locus_tag="U1099_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868521.1"
                     /translation="KMPNINGIKRVQSFNSDVSVNFLLDDESRDFLKENLPLTGVVIY
                     EPTLKKLAENIIILNRQKHRMSDESRISLMNKEIYQGYRETSFYTSIIEA"
BASE COUNT          324 a          156 c          126 g          289 t
ORIGIN      
        1 attatcagac gtaatatcaa aatgtaatgt agcactgcta ataactacat tgttttcttc
       61 aacatacaaa ttcaagctaa ccaacatatc gaatagagat tctctaacat acaatatatt
      121 tttaattttt gatataaaaa cagacttaaa aaatttttca atagaacaaa ttattccaat
      181 aaatatcaaa gaaagcactg atttaacagg gattgagtat ggagaaaaca aagtatttat
      241 caataaaata ccgagaaaaa attccattcc aactctgata caagaaattc ttcgagaagt
      301 tatgatgtaa gggttaaact ttacccgtac cgtttgattc agcttgatta tcttcattat
      361 cccctccctt atcattttca actgatagat aatctaataa tatcaatagg agtggtatga
      421 ctaaaaatcc tcctgataaa aataaggtat cgaaccatgg agacatctcg cccttcatct
      481 ggaatataat atacaagcaa agaattagag caggataagt gatgttgaac ataaacttat
      541 aaattttcat aataataatt tcctttcata aaaccaacgg gggaggagtt agctcccctt
      601 atatcatcac taagcttcaa ttattgaggt gtaaaaagaa gtttccctgt agccttggta
      661 aatctctttg ttcatcaagc taatcctaga ttcatcagac atacggtgtt tttgacgatt
      721 aagaataatg atgttttctg ctaacttctt caaggttggc tcatagatta ctactccagt
      781 aagtggcaaa ttttccttta aaaaatctct ggattcatca tctaagagaa agtttaccga
      841 tacatctgaa ttaaagcttt gtacccgctt aatcccattg atattcggca ttttc
//
LOCUS       DASFSW010000294          873 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.298, whole genome shotgun sequence.
ACCESSION   DASFSW010000294 DASFSW010000000
VERSION     DASFSW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.298"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(35..>873)
                     /locus_tag="U1099_002259"
     CDS             complement(35..>873)
                     /locus_tag="U1099_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEM4868522.1"
                     /translation="ISSVYLDVAPNMKKLPSISDLYPAENRVAIHPYFDTSYNRNSIY
                     KQLGIEQFYTLNSAKYPLAVTAEDYQGNFVSDKKTYDLILEQVRSGTNTFVSAITMQN
                     HVQWNSAEPASITASGEGFTAEENANLNSYVRLLSFTDQATRDFLDQLKTLDKKVTVV
                     FYGDHLPGLYPESAFVTDPSAQYKTDYFVWSNFETEDYHYDLVNSSDMNALMLETTNN
                     KVSPYYALLTEVLHKDRVGQAERDAKVAEELKLVQYDLSTGKGYLLKYKDFFKVATET
                     TE"
BASE COUNT          231 a          202 c          179 g          261 t
ORIGIN      
        1 ttgaccagtc gagttctttc ccactcccct atttttattc tgtcgtttca gtcgctactt
       61 tgaaaaaatc tttgtatttc aaaaggtagc cttttcctgt ggataggtca tattggacca
      121 gtttgagttc ttctgcaact ttcgcatctc gctcagcctg tcctactcgg tccttatgaa
      181 gtacttctgt caagagggca tagtaaggac tgaccttgtt attggtcgtc tccagcatca
      241 gtgcattcat gtcgctagaa ttgaccaaat cataatggta gtcttctgtt tcaaaattac
      301 tccacacgaa ataatccgtc ttgtactgcg cacttgggtc cgtcacgaaa gctgactctg
      361 gatacaaacc tggtaggtgg tcaccataaa aaacaacggt cactttctta tccaaggtct
      421 tgagctggtc tagaaaatcc ctggttgcct gatccgtaaa ggatagaaga cgaacatagc
      481 tattgaggtt ggcattctcc tcagccgtga aaccctcacc cgaagctgta atacttgcag
      541 gctcagcaga gttccactga acatggttct gcatggtaat ggccgagaca aatgtatttg
      601 ttcctgaacg tacctgttcc aaaatcaaat cataggtctt tttatcactg acaaaattcc
      661 cctgataatc ttcagccgtc actgcaagtg gatacttggc actattgagc gtatagaact
      721 gctcaatccc caactgctta tagatagaat tacgattgta actggtatcg aagtaggggt
      781 gaatagcgac acgattctct gctggataga ggtcactgat acttggcaac ttcttcatat
      841 ttggcgccac atccaaatag actgaagaaa tgg
//
LOCUS       DASFSW010000295          842 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.299, whole genome shotgun sequence.
ACCESSION   DASFSW010000295 DASFSW010000000
VERSION     DASFSW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.299"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..189)
                     /locus_tag="U1099_002260"
     CDS             complement(<1..189)
                     /locus_tag="U1099_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4868523.1"
                     /translation="MQKLQTVNAETLLYEPLEKPSFVVDSLIPTGLSLFCGSQKIGKS
                     WLMLKLCLCVSQGIPLWDM"
     gene            complement(344..818)
                     /locus_tag="U1099_002261"
                     /pseudo
     CDS             complement(344..818)
                     /locus_tag="U1099_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331637.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          196 a          230 c          177 g          239 t
ORIGIN      
        1 catatcccat aaagggattc cctgcgacac gcataagcat agcttcagca tgagccagct
       61 tttgcctatc ttctgtgagc cgcagaacag cgataagcct gtcgggataa ggctgtccac
      121 cacaaaggat ggtttctcaa gcggttcata aaggagcgtt tcggcgttga ctgtctgtaa
      181 cttctgcatg gctttcctcc tttcgggcgg tgtctttgtt ttcgttgcac ataggcgttg
      241 acctccttaa aaatagattt actcccacgg aaaaaagtga gagtatgtaa tgccgccaat
      301 cccacacaaa taaaaaacag atttcttttt cgggcggtgt cggtcacggt tggagatatt
      361 tcttcaattt ccgcctttac tttctccttt gcaatcgcaa cagatttctg tattgccgaa
      421 gcggtgcaat gctccatagc ggcgatttgg taaaaattga actcatactc gtagtagagc
      481 aggaaacgcc gcctttgtat ctctggaagg ctcccaaccg ccttatagag cgtttcattc
      541 cgttcttcct caaccatgcg ttcatcaagg ctcttaggca cacgcaacgc ccgtctgtaa
      601 agggtttcgt cccatacctc gttaaactcc ctgtgccgct cgtcccatta gaaaagattc
      661 ctgttcctgc gctccatctg ccgaaactcc ataaagaact gttccgacac ttccaactcg
      721 tgggatttgc cctgcccgtc cttaaagctg ataaaatacc ttgtgccgct ttccgtggat
      781 tcctcccgaa gcgtgtatgc cttaaccctg tatgccatcc tgttgtcctc ctgcaaaaaa
      841 tg
//
LOCUS       DASFSW010000296          839 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.300, whole genome shotgun sequence.
ACCESSION   DASFSW010000296 DASFSW010000000
VERSION     DASFSW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.300"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>839)
                     /locus_tag="U1099_002262"
     CDS             complement(<1..>839)
                     /locus_tag="U1099_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM4868524.1"
                     /translation="TGDFEQAKIVEETYQPFRYYLREEPFDQARTYLNDIRGLSDDTI
                     NAFGRQGLLAQAHYQTKTFQESVLVFKSYNHLGQLEGASLQGLVKDNQRHDRGYLKKI
                     MKDSHGYIGMSFDIGKPKRLIFCESVIDMMSYYQLHQKQLSDVRLVSMEGLKLSVIAY
                     QTLRLAAEEQGKLEFLDTVKPSRLTHYLHAIQETTTFFQTHPGLLTLAVDNDEAGRDF
                     CQKLSEKGLPTETDCPPLQELETKADWNDIVKYQNNYSLKDVIQSAKLQVIRSNPPPR
                     KNT"
BASE COUNT          233 a          166 c          161 g          279 t
ORIGIN      
        1 ggtatttttt ctaggcggag gattgcttcg gataacttgt aacttggccg attggatcac
       61 atcttttaga gaataattat tttgatattt tacaatatca ttccaatctg ctttagtttc
      121 cagttcctgt aatggtggac agtctgtttc agtgggaagt cctttctcag ataatttctg
      181 acaaaaatct ctccctgctt catcattgtc aacagccaag gttaataagc ctgggtgcgt
      241 ctgaaagaag gtagtcgtct cttgtatagc atgaaggtaa tgcgttaatc gacttggttt
      301 gacagtatct aaaaactcaa gtttgccttg ttcctcagct gctaatcgta aagtctgata
      361 cgcaatcaca gagagtttta agccttccat agaaaccaaa cgaacatctg ataactgctt
      421 ctgatggagt tgataataac tcatcatatc aatcaccgat tcacaaaaga taaggcgttt
      481 cggttttcca atatcaaagc tcatccctat atagccatga gagtctttca tgattttctt
      541 gaggtaacct cgatcatgtc tctgattatc tttgacaagc ccttgaagac ttgccccctc
      601 tagttggcca agatgattgt aactcttaaa gacaagaact gattcctgaa aggtctttgt
      661 ctggtaatga gcctgtgcta atagaccttg tctgccaaaa gcattgatag tgtcatcact
      721 taaaccacga atatcattta agtaagttcg tgcttggtca aatggttctt ctcttagata
      781 ataacgaaag ggttggtaag tctcttcgac gattttagct tgttcaaagt ctccagttt
//
LOCUS       DASFSW010000297          833 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.301, whole genome shotgun sequence.
ACCESSION   DASFSW010000297 DASFSW010000000
VERSION     DASFSW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.301"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            1..833
                     /locus_tag="U1099_002263"
                     /pseudo
     CDS             1..833
                     /locus_tag="U1099_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          286 a          161 c          188 g          198 t
ORIGIN      
        1 cgaggaactc tcccaaactg gatagcacaa tacaagaaaa acgggtatac tattcttgag
       61 aaaacaagag ggaggccacc aaagatgggg cgtaaaccaa agaaaacctg ggaagagatg
      121 acggagttag agcgacttca ggaggaaaat gaacggctta gaacggagaa tgtctatcta
      181 aaaaagttga gagcgcttcg tttaagggac gaagccagag aacaagaaag gctcaaacag
      241 ttagagagat ggtcgcagaa ggatttcgac tagatgtact actgacgatt gctcaattag
      301 ttccttctac ctatcattat caagtcaaac aactggacaa accagataaa gataaggact
      361 taaaagctga aattcaagcc atttatgatg aaaataaggg aaattatggc taccgtcgca
      421 ttcaccttga gctaagaaat cgtggatttt ctgtcaacca caagaaagta caacggctga
      481 tgaaagagtt aggtttgact gctcgtattc gtagacgcaa gagatacaag tcttacaagg
      541 gtgatgtggg caagaaagct cctaatctca ttgagagagc ctttgaaggg gctaagcctt
      601 ttgagaagtg ctatacagat gtgactgagt tcgctctgcc aaattgtgct gaaaaactct
      661 atctttctcc agttctggat ggctataata gcgaaattat tgattttacc ttatcccgct
      721 ctccaaacct catacaagtc aagaccatgc ttgagaaggt ctttcctgag gaaacttacc
      781 caaataccat tctccatagt gaccaaggtt ggcaatacca acatgaagtc tat
//
LOCUS       DASFSW010000298          800 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.302, whole genome shotgun sequence.
ACCESSION   DASFSW010000298 DASFSW010000000
VERSION     DASFSW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.302"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            14..>800
                     /locus_tag="U1099_002264"
     CDS             14..>800
                     /locus_tag="U1099_002264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868525.1"
                     /translation="MQDYKEWWEITKEYRELDLLNSQFSENFSEELSILEYRPLFYKF
                     FQNKQLKHWSKDGFEFLTSKICQLESQLGHEQIVNVFLNNLDLWISMYEQLLDIEERI
                     NLMELFNGSEELRAKLFAVGIYNDLLNSAYSNGLKLIIKFHDEILGKNQDQKTLTPQI
                     EYLRKRDYNLITDISDSDVRNAISHGGIRYNNTTLSFHFTQGSIVGQKEISTFQFITT
                     IKEIFDITSSMCLSWLYYWSQNISLDQLRNFNSLSKISIKFIDK"
BASE COUNT          291 a           95 c          137 g          277 t
ORIGIN      
        1 gttaggaggt ataatgcaag attacaagga atggtgggaa attactaaag aatatagaga
       61 attggacttg cttaattctc aattctctga aaatttttca gaagagttgt ctattttaga
      121 gtataggcca cttttttata aattttttca aaataaacaa ttaaaacatt ggagtaaaga
      181 tggttttgaa tttctgactt caaaaatttg tcaactggaa agccagttgg gacatgaaca
      241 aattgtgaat gtatttttaa ataatctaga tttgtggatt tctatgtatg aacaactgct
      301 tgacatagaa gagagaatta atctcatgga attattcaat ggtagtgaag aattacgggc
      361 taaactattt gcagttggta tatataatga tttgttgaat tcggcttata gtaatggtct
      421 taaattaatt atcaaatttc atgatgaaat tttaggtaaa aatcaagacc aaaaaacttt
      481 aactcctcaa attgaatacc ttaggaaacg tgactataat ttaataacag atatctcaga
      541 ttcggatgtt cgtaatgcta tatctcatgg aggaattaga tacaataata ctacgttgag
      601 tttccatttt acccagggga gtattgtagg gcaaaaagaa atttcaacat ttcaatttat
      661 cacgactatt aaagaaatat ttgatattac tagtagcatg tgtttatcgt ggctatatta
      721 ttggagtcag aatatctccc tagatcaatt acgaaatttc aatagtttat caaaaatatc
      781 tatcaagttt attgataaac
//
LOCUS       DASFSW010000299          797 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.303, whole genome shotgun sequence.
ACCESSION   DASFSW010000299 DASFSW010000000
VERSION     DASFSW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.303"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..646)
                     /locus_tag="U1099_002265"
     CDS             complement(<1..646)
                     /locus_tag="U1099_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868526.1"
                     /translation="MFSKLSDHDFKKGVFRTKFSQIVTPLDKESDWIHSKGPEYLWIG
                     LALQYGSRTEQMERMMLALTNLSKELDLEKILPLPAMSLILSLDIEEKKIFVESLNSA
                     FDLSIFSPLSIVLPDGEEVLSKNFYNRSHSFDQRLDVLVKVLDEISDQYSQLSTDVRY
                     FLLYYKMLQGKIKFVESQSHMVDGLTKYPYLDISDPEMKIIRSQIRSMEVALSMS"
BASE COUNT          268 a          137 c          102 g          290 t
ORIGIN      
        1 cagacattga taaagcaacc tccattgagc gaatctggga acgaataatt ttcatttctg
       61 gatctgatat atctaagtaa gggtatttag ttaatccgtc taccatgtgt gactgcgatt
      121 ctacaaattt aatttttcct tgtagcatct tgtaatacag taaaaaatat cttacatccg
      181 ttgacaactg actatattgg tcagatattt catctaatac tttaacaagt acatctagtc
      241 tttgatcaaa agaatgactc ctgttgtaaa aatttttact cagaacttct tcgccatccg
      301 gcaagactat tgaaagtgga gaaaatattg ataagtcaaa agctgaattt aatgattcaa
      361 caaaaatttt cttctcttca atatctaaac taagtattaa actcattgca ggtaaaggca
      421 agattttttc taaatctaat tctttagata aattagtcag agctaacatc attctttcca
      481 tttgctctgt tcggcttccg tattgcagag ctaatccaat ccaaagatat tctggacctt
      541 ttgaatgtat ccaatcagat tctttatcta atggagttac tatttgactg aatttagttc
      601 taaagacccc ttttttaaag tcatgatcac ttaacttact aaacataaga tacctcacaa
      661 tacaatgtta tcactattgt tatctattcc caaaatatac tcacttctaa tttttattat
      721 tcaatcttga ttacaaaaat atttgtttga taaataaact attataactt ggaatagtta
      781 gtgaatattc tactgta
//
LOCUS       DASFSW010000300          796 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.304, whole genome shotgun sequence.
ACCESSION   DASFSW010000300 DASFSW010000000
VERSION     DASFSW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.304"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            19..>796
                     /locus_tag="U1099_002266"
     CDS             19..>796
                     /locus_tag="U1099_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEM4868527.1"
                     /translation="MVSGKKVFIFGILGLALGYFCHRLVLLYDSLPNQPPLERLAYLL
                     GEGQNQVLNPLWNGNFTGKSILGFCFGFVTMGLVYLYVSTGQKVYREGAEYGSARFGN
                     NRERKAFLSKNPFNDTILSRNVRLTLLEKKAPQFDRNKNLVVIGGSGSGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLEHGYQVKVIDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAISSYLVDFY"
BASE COUNT          209 a          155 c          180 g          252 t
ORIGIN      
        1 aaaggaggtc attctctaat ggtctcaggg aaaaaagttt ttatttttgg tattttaggg
       61 cttgctcttg gctatttctg ccatcggcta gtcttgcttt acgatagtct acctaatcaa
      121 ccgcccttgg aacgtcttgc ctatctctta ggagaaggac agaatcaggt cttaaatccc
      181 ttgtggaatg ggaactttac aggtaaatca attttgggct tttgctttgg atttgtgacg
      241 atgggcctag tctatcttta tgtttcaact ggtcaaaagg tttaccgaga gggggctgaa
      301 tatggctctg ctcggtttgg aaacaatcgt gagcgtaaag cctttctcag taagaatccc
      361 tttaatgaca ccattttgtc acgaaacgtt aggctaaccc tgttagaaaa gaaagctccc
      421 cagtttgata gaaacaagaa ccttgtggtc attggtgggt ctggtagcgg gaaaaccttt
      481 cggtttgtca aacccaatct gattcagctc aactgttcca atattgtcgt tgaccctaag
      541 gatcacttag ctgaaaagac agggaagctc tttttagagc atggctatca ggtcaaggtc
      601 atagatcttg taaacatgac aaactcagat ggttttaatc cttttcgtta tgtggagacg
      661 gagaatgatt tgaaccgtat gcttacagtc tatttcaata ataccaaagg caatggcagt
      721 cgcagtgatc ccttttggga tgaagcttcc atgaccttag tcagagccat ttcctcttac
      781 ttggtggatt tttaca
//
LOCUS       DASFSW010000301          793 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.305, whole genome shotgun sequence.
ACCESSION   DASFSW010000301 DASFSW010000000
VERSION     DASFSW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.305"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..414)
                     /locus_tag="U1099_002267"
     CDS             complement(<1..414)
                     /locus_tag="U1099_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4868528.1"
                     /translation="MAYKAKAYTLREESTESGIRYFISFKDGQGEHHELEVSEQLFFE
                     FRQMERRNRNLLQWDERHREFSEVWDETLNRRALKLPKSIEEQMIEAERAELLCKAVD
                     RLPEIQRRRFLLYYEYEFNFYQIAAMEHCTASAIQK"
     gene            complement(498..>793)
                     /locus_tag="U1099_002268"
     CDS             complement(498..>793)
                     /locus_tag="U1099_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4868529.1"
                     /translation="NSNRRFYRWTNDVAEYLEDMADRERQAEVNTVAELLDEIESENL
                     YQVLVTVDGRTLKIVLLKMQGYSTKEIAPLVHLTTGAIYARLDHLRKKLRKIL"
BASE COUNT          177 a          233 c          159 g          224 t
ORIGIN      
        1 tttctgtatt gcagaagcgg tgcaatgctc catagcggcg atttggtaaa aattgaactc
       61 atactcgtag tagagcagga aacgtcgcct ttgtatctct ggcagtctgt caaccgcctt
      121 acagagcagt tccgcccgtt ctgcttcaat catttgttcc tcaatgctct taggcagctt
      181 taacgcccgc ctgttcagcg tttcgtccca tacttccgaa aactccctgt gccgctcgtc
      241 ccattggaga agattcctgt tcctgcgttc catctgccga aattcaaaga ataactgctc
      301 ggatacttcc agttcgtggt gttccccctg cccgtcctta aaactgataa aataccttat
      361 accgctttcc gtggattcct cccgaagcgt gtacgcctta gctttatatg ccatcccgtt
      421 ttcctcctgc caaaaataag cggggagatt ccactcccca cccagtagcc cgcagaataa
      481 tgggatgtat tagacgctta caaaattttc cgcagcttct tccgcagatg gtctaacctc
      541 gcatagatgg caccagtcgt caaatgcaca agtggggcaa tctcctttgt ggaatacccc
      601 tgcattttca gcaggacgat tttcaaggta cgcccgtcca ccgtgactaa tacttgatag
      661 agattttcgc tctcaatctc gtccagtaac tccgcaactg tattcacctc cgcttgccgc
      721 tccctgtctg ccatgtcctc aaggtattcc gcaacgtcat tcgtccatcg gtaaaaccgc
      781 ctgttggaat tga
//
LOCUS       DASFSW010000302          787 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.306, whole genome shotgun sequence.
ACCESSION   DASFSW010000302 DASFSW010000000
VERSION     DASFSW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.306"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>787
                     /locus_tag="U1099_002269"
     CDS             <1..>787
                     /locus_tag="U1099_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM4868530.1"
                     /translation="KSRIINDNLWVSTIHEFIWHIIGNFQKEIKECLVELINDDSQKL
                     FVSPKDVIEEESISLDYFDDVRVDYAEWYSMSITEQNKVQISHDHILIVAEKMFSKYK
                     KLCDVLADTANYIFVDEYQDTSPLIVKILLEHLQKSTKKNVVGFFGDSMQAIYDSGVG
                     DLNSYNLAKIQKTQNRRNPKKVIDLANKVRNDGLVQTPSNDDTAPNMDDGSVIAGTVK
                     FVYGNDINQLDVLRNSKLYKALEFDIAKKTKELRLTHKLNAEMA"
BASE COUNT          299 a          100 c          141 g          247 t
ORIGIN      
        1 aaatcacgaa taattaatga taatttatgg gtatcaacaa ttcatgagtt tatctggcat
       61 atcattggaa atttccaaaa agagattaaa gaatgtcttg ttgagttaat aaatgatgat
      121 tcacaaaagt tatttgtttc accaaaggat gtaattgaag aagaaagtat ttcacttgat
      181 tactttgatg atgtccgtgt tgactatgct gaatggtact ctatgagtat aacagagcaa
      241 aataaagtcc aaataagtca tgaccatatt ctaatagttg ccgaaaaaat gttttcaaaa
      301 tataagaagt tatgtgatgt tttagcggat actgcaaact atatttttgt tgacgaatat
      361 caagatacaa gtccactaat tgtgaaaata ttattagagc atcttcaaaa gagtactaag
      421 aaaaatgttg taggcttttt tggagattct atgcaagcta tttatgattc aggggtagga
      481 gacttaaatt cttataattt ggcaaagata caaaaaactc aaaatcgtcg taatcctaaa
      541 aaagttattg atttagccaa taaagttaga aatgatggtt tagttcaaac tccatccaac
      601 gatgatacgg ctcccaatat ggacgatggg agtgtcattg ctggaacagt gaaatttgtc
      661 tacgggaacg atattaatca acttgatgtt cttagaaata gtaaactata taaagcttta
      721 gaatttgata tagctaaaaa aacaaaagaa ttacgtctga cacataaact taatgcagaa
      781 atggcag
//
LOCUS       DASFSW010000303          772 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.307, whole genome shotgun sequence.
ACCESSION   DASFSW010000303 DASFSW010000000
VERSION     DASFSW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.307"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..625
                     /locus_tag="U1099_002270"
     CDS             <1..625
                     /locus_tag="U1099_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEM4868531.1"
                     /translation="LPTMMQKVDIATPIEQGSNIMPLLSQNISIIETGAHSQVFRKFF
                     DFLGIKVLIITDIDPVNKNEKNRFIACSAVNATSTSNTSLKSFFNISGDGIFSTVTQK
                     SFAEKITPDNRIRIAYQIPEDNNGYQPASFEDSFIGLNKEFIIKQKDGLIEYEALKNF
                     DDDEIEDFYTFARNKVNKKSAFASSLLYFEDEENTWKVPKYISEGLL"
BASE COUNT          302 a          102 c          126 g          242 t
ORIGIN      
        1 actgccaaca atgatgcaaa aagtggatat agcaactcca atagaacaag gttcaaatat
       61 aatgccatta ctttctcaaa acatttcaat cattgaaaca ggtgctcatt cacaagtgtt
      121 taggaaattt tttgactttt tagggataaa agtattgata attactgata ttgatcctgt
      181 aaataaaaat gaaaagaatc gttttatcgc ttgttcagca gtaaatgcta catctacgtc
      241 aaatacttct cttaaaagtt tcttcaatat ttcaggagat ggaatttttt caacagtaac
      301 tcaaaaaagt tttgctgaaa aaataacacc agataataga atccgtattg catatcaaat
      361 tcccgaagat aacaatggtt atcagcctgc tagttttgaa gattccttta tcggtttaaa
      421 taaagaattc attattaaac aaaaagatgg tcttattgaa tatgaagctt taaaaaactt
      481 tgatgacgat gaaatagaag atttctatac atttgctagg aataaagtga acaaaaagtc
      541 tgcctttgca tcaagtttat tatactttga agatgaagaa aatacttgga aagtacctaa
      601 atatatttcg gagggattat tatagttacg agaacagtag agcaagaagt agaaaaagta
      661 tttgaagcaa taaaacaagg tcaaaatttt attcttgaag gcggtgctgg aagtggtaaa
      721 acttattcgc taatttcaat tattgaaaaa atattgattg aagaacctaa aa
//
LOCUS       DASFSW010000304          752 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.308, whole genome shotgun sequence.
ACCESSION   DASFSW010000304 DASFSW010000000
VERSION     DASFSW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.308"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>752
                     /locus_tag="U1099_002271"
     CDS             <1..>752
                     /locus_tag="U1099_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM4868532.1"
                     /translation="PATITNMISYLVAENKVIETGDEVRDYIGSGRSRRLITVNSQYR
                     HYIGIEINANGIYLAVTDTIGNLIKNSNIKITEYDSKKINEIIIQLVTETLEKFSYLE
                     FGALGIAVPGHFDSKSGHIISNNVKWIYFDLAVIKESISIPIFLENNINCMAIGAYLF
                     HPESSPEQFLFIHIGPGLFCSFFDSEHILQNKNFYIGEIGHTVVDLNGPSCECGKRGC
                     LQTYISDTWLINNARFLFENVQGSIIKTLVEKP"
BASE COUNT          262 a          107 c          126 g          257 t
ORIGIN      
        1 ccggcaacta ttacgaatat gattagttat cttgtagctg aaaataaagt tattgagact
       61 ggcgatgagg ttcgagatta tattggatca ggtcgtagta gacgacttat tacagtaaat
      121 tctcaatata gacattatat tggaatagag attaatgcca atggaatcta tttagcagtc
      181 acagacacta ttggaaacct aatcaaaaac agcaatataa aaattacaga atatgattcc
      241 aaaaaaataa atgaaataat aatccaatta gtaaccgaaa ctctagaaaa gttttcctat
      301 cttgaatttg gagctctcgg tattgctgta ccaggccact ttgattcaaa atcaggacat
      361 attatttcaa ataatgtaaa gtggatttat tttgatcttg ccgtaattaa agaaagtatt
      421 tcaattccta tttttctaga gaacaatatt aattgcatgg caattggtgc atatcttttt
      481 catcccgaaa gttcgccaga gcagttttta tttattcata ttggacctgg attgttctgt
      541 tcattttttg atagcgaaca tattttacaa aacaaaaact tttatattgg agagattggt
      601 catacagtag ttgatttaaa cggaccttct tgtgaatgtg gaaaacgcgg atgccttcaa
      661 acttatattt ctgatacttg gttaattaat aatgcaagat ttttatttga aaatgttcaa
      721 ggttctatca taaaaacttt ggttgaaaaa cc
//
LOCUS       DASFSW010000305          729 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.309, whole genome shotgun sequence.
ACCESSION   DASFSW010000305 DASFSW010000000
VERSION     DASFSW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.309"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            121..588
                     /locus_tag="U1099_002272"
     CDS             121..588
                     /locus_tag="U1099_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEM4868533.1"
                     /translation="MSTNRLAWDEYFAAQALLIANRATCKRAKVGAVLVKDNKVIATG
                     YNGSVSGTEHCLDQECLMIDGHCARTLHAEVNAILQGAERGIPKGFTAYVTHFPCLNC
                     SKQLLQVGCKRVVYINEYRMDDYAQYLYKEKGCELVHLPLEVVKQAIADTEFI"
BASE COUNT          210 a          130 c          170 g          219 t
ORIGIN      
        1 acatagtcaa gtgtttatgc atccaaggta ttcattcaat gaatcaactt tttatcattt
       61 ttaaaagcac aaatagttgg tggtgagcag tgatttataa aaatatataa aggagaaatc
      121 atgtcaacaa atcgtttagc ttgggatgag tattttgcgg ctcaggcact tttgattgcc
      181 aatcgggcga cctgtaagag agccaaggtc ggtgcggttt tggttaagga caataaggtt
      241 atcgcaacag gctacaatgg ttctgtttca ggaaccgaac attgtttgga tcaggaatgc
      301 cttatgattg atggtcattg cgctagaacc ttgcatgcgg aagtcaatgc cattttacaa
      361 ggagcggagc ggggaattcc aaaaggtttt actgcctatg tgacccattt cccatgtctc
      421 aactgctcca agcaattgtt acaggttggt tgtaagcggg ttgtttatat caatgaatac
      481 cgcatggatg actatgctca gtacttgtat aaagaaaagg gctgtgagtt ggttcatttg
      541 cctctagagg tggttaaaca ggcaattgca gataccgaat ttatctaatg attttgtaga
      601 agagtgggag tgagaaagaa ctcgaccatt catagaagag ttcgtcaaca atttttcgtt
      661 ttaagttgtt gagctgaaac agtctatcct cagactgttt cactcccacc cccgcatagc
      721 tccaacagt
//
LOCUS       DASFSW010000306          729 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.310, whole genome shotgun sequence.
ACCESSION   DASFSW010000306 DASFSW010000000
VERSION     DASFSW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.310"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..568)
                     /locus_tag="U1099_002273"
     CDS             complement(<1..568)
                     /locus_tag="U1099_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4868534.1"
                     /translation="MEKVSIIVPIFNTEKYLRECLDSIISQSYTNLEILLIDDGSSDS
                     STDICLEYAEQDGRIKLFRLPNGGVSNARNYGIKNSTANYIMFVDSDDIVDGNIVESL
                     YTCLKENDSDLSGGLLATFDGNYQESELQKCQIDLEEIKEVRDLGNENFPNHYMSGIF
                     NSPCCKLYKNIYINKGFDTEQWLGEDLLF"
BASE COUNT          234 a          154 c           86 g          255 t
ORIGIN      
        1 taaataataa gtcctctcct aaccactgtt cagtgtcaaa acctttgttt atatatatat
       61 tcttataaag tttgcaacaa gggctattaa agataccgct catataatga tttggaaaat
      121 tttcatttcc taagtctcgc acctctttta tctcttccaa atcaatttga cacttttgca
      181 gctcagattc ttgataattt ccatcaaaag tagcaagtaa ccctcccgac aaatcactat
      241 cattctcttt taaacaggtg tataaggact caacaatgtt gccgtcaaca atatcatcag
      301 aatctacaaa cataatataa tttgctgtgc tatttttgat accgtaattc cttgcgtttg
      361 aaacaccacc atttggtaac cggaaaagtt ttattctacc atcttgctct gcgtattcca
      421 aacatatatc cgttgatgaa tctgaagaac cgtcatctat caaaagaatc tctagattag
      481 tatacgattg ggaaataatg ctatctaaac actctcttaa gtacttttcc gtattaaaaa
      541 taggtacaat aatgctgact ttttccatcc tctaattcct tttatgccgt aacttgcttt
      601 gctatcataa aaatcatcat gtaaaaaata ctatcttcta tcttgccacc tatagaacat
      661 atgctctacc aaattaatag caactcaaaa ctctattcct gccaatttat gtttgcagtt
      721 gtttgttaa
//
LOCUS       DASFSW010000307          716 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.311, whole genome shotgun sequence.
ACCESSION   DASFSW010000307 DASFSW010000000
VERSION     DASFSW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.311"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>716)
                     /locus_tag="U1099_002274"
     CDS             complement(<1..>716)
                     /locus_tag="U1099_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM4868535.1"
                     /translation="LYQGLFPNGLMQVTPDYFSQSYLLGDVNYQTVGLEDKGAIMETY
                     SDLINSLDDKTNFQLTIFNQRVNLDKFRKSVLYPLHEDGYDTYREELNRIMENNLEAG
                     ENNFSAVKLISFGKSDQNPKLAYRSLSQIGEYFKSGFSEIDAGFTLLSGESRVNHLTD
                     MLRGENHLSFTYQDLMRSGQTTKHFIAPTSLSFKHKNYVEIDDRLIQIVYVRDYGMEL
                     GDKFLRELMQSDLEVMISLH"
BASE COUNT          221 a          151 c          135 g          209 t
ORIGIN      
        1 atgaagacta atcatcactt ctaaatccga ctgcatgagt tctcgtaaga acttatcccc
       61 caactccatc ccatagtcac gaacatagac aatctgaatc agtctatcgt caatctctac
      121 ataattctta tgtttgaaag ataaactggt tggggcaata aagtgcttgg ttgtttgtcc
      181 tgaacgcatc aggtcttgat aagtaaagga caaatgattt tcccctcgca acatatcagt
      241 caagtgattc acacggcttt ctccactgag gagggtaaac ccagcatcta tttctgaaaa
      301 accactcttg aagtattccc caatctgaga cagggagcga taagcgagtt tcggattttg
      361 gtctgacttc ccaaaagaaa tgagcttaac cgctgaaaag ttattttcac cagcttcaag
      421 gttattctcc atgatacgat tgagttcttc acgataggta tcgtagccat cttcatgtag
      481 tggatataag acactttttc taaatttatc caagttgacc ctctggttaa aaatagtaag
      541 ttggaaattg gtcttgtcat ccaaactgtt aatcaagtcc gaataggttt ccataatggc
      601 tcccttatcc tccaaaccaa ccgtttggta attgacatct cctaaaagat aggactgaga
      661 aaaataatct ggggtgactt gcatgaggcc attaggaaaa aggccttggt aaagaa
//
LOCUS       DASFSW010000308          695 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.312, whole genome shotgun sequence.
ACCESSION   DASFSW010000308 DASFSW010000000
VERSION     DASFSW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.312"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(70..>695)
                     /locus_tag="U1099_002275"
     CDS             complement(70..>695)
                     /locus_tag="U1099_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM4868536.1"
                     /translation="KARLLIGLGGLAVTSFMVMMGYTIGSQSVTNHTQKQIQSEAQKL
                     LTKEKEKEKASVLSDELVKEFLTQYYTKEKLGENNNRIKPYMTDSAFKEEVARQEDSI
                     NQVYKDYMLDYRFESANIYVNAEENEAFAEVSYQVVYVSDVSDKAHKSTQTETRTLKL
                     SYAKLSDKLFVNQVTIWNGKLEDLKESSDEVNSSIPKIQGTTTSETN"
BASE COUNT          184 a          147 c          122 g          242 t
ORIGIN      
        1 cttttgtttc actttttcaa ttcttgtcat gccatctcct tatcaaaaaa agcagaagag
       61 taactcctgc tagttggtct cacttgtagt agtgccttga atctttggaa tgcttgaatt
      121 tacttcatca gaagactctt ttaagtcctc tagcttccca ttccaaatgg ttacctgatt
      181 gacaaagagt ttatctgaca atttggcata agatagttta agagttcttg tttctgtctg
      241 tgtgctttta tgagccttat cactgacatc tgacacgtaa acaacctgat aagacacttc
      301 cgcaaaagct tcattttctt ctgcattgac atagatattg gcagactcaa agcgataatc
      361 caacatatag tctttataga cttggttgat ggaatcttct tgacgagcta cttcttcctt
      421 aaaggcagaa tctgtcatat agggtttaat acggttgtta ttctcgccca gcttttcctt
      481 agtatagtat tgggtgagaa attccttgac taactcatca gagaggacac ttgccttctc
      541 tttttctttc tctttggtca gtaatttttg agcctcagat tggatttgtt tctgcgtgtg
      601 attggtaaca gactgagaac caatggtgta acccatcatc accataaagc tagtcacagc
      661 tagacctcct aaaccaatca atagccgagc cttca
//
LOCUS       DASFSW010000309          691 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.313, whole genome shotgun sequence.
ACCESSION   DASFSW010000309 DASFSW010000000
VERSION     DASFSW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.313"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            230..415
                     /locus_tag="U1099_002276"
     CDS             230..415
                     /locus_tag="U1099_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868537.1"
                     /translation="MSQPIVPLTILQSRRFEKKSRNDILMKIRLGKTEVIFFQSINPK
                     IMETILDKVLHYDHPTQ"
     gene            396..>691
                     /gene="tnpB"
                     /locus_tag="U1099_002277"
     CDS             396..>691
                     /gene="tnpB"
                     /locus_tag="U1099_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEM4868538.1"
                     /translation="MTIRLSDLGQVFLVCGKTDMRQGIDSLAYLVKSQFNLDPFSGQV
                     YLFCGGRKDRFKALYWDGQGFWMLYKRFENGKLTWPNNEEEVKALTSEQVDWLM"
BASE COUNT          209 a          146 c          147 g          189 t
ORIGIN      
        1 gaatgttagt gcttataagg gggggaacag aatcctctct tgattgaaga caagctagtc
       61 attaggctgg tttgtctttt ttaggctctt tgtcaactgt agtgggtaga ctaaaagcta
      121 acacctagag aggacgaaat tcgttcgtaa ccgcccaata acaaggtatc tatccaatca
      181 ctcattcctc cagtatactg ttataaaaaa acaaactgga ggatttatta tgtcacagcc
      241 catcgtccca ttgactattc tccaatctcg ccgctttgaa aagaaaagca gaaacgatat
      301 tctgatgaaa attcgtctcg gaaaaacgga agtcatattc ttccaatcaa tcaaccctaa
      361 aataatggaa acaatcttgg ataaggtgct gcattatgac catccgactc agtgatttag
      421 gccaagtctt cttggtttgc gggaaaacgg atatgcgtca agggattgac tctctcgcct
      481 accttgtcaa aagtcaattc aatttggatc ccttctccgg tcaggtctat ctcttctgcg
      541 gcggtcgaaa agaccgattc aaggctcttt actgggacgg acaaggattt tggatgcttt
      601 acaaacgttt tgaaaatggg aaattgacct ggccaaacaa tgaagaggag gtcaaggccc
      661 taacttccga acaagtagac tggctcatga a
//
LOCUS       DASFSW010000310          684 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.314, whole genome shotgun sequence.
ACCESSION   DASFSW010000310 DASFSW010000000
VERSION     DASFSW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.314"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            445..>684
                     /locus_tag="U1099_002278"
     CDS             445..>684
                     /locus_tag="U1099_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEM4868539.1"
                     /translation="MKTITHKELMKLGFAKTTARKIIRQAKNIAIQRFEVADKNSVNA
                     VKLSKSPFDNRRLDLAPLSIVEELLGFSLVEYEEKN"
BASE COUNT          256 a          106 c          134 g          188 t
ORIGIN      
        1 acgtcaagtt gctgaagagt taaaaactag cccatgcaga atctctagat taagagataa
       61 agtccttaat aagttaaaaa acatgcttga aaattcgcat aaaatgtgaa acagaccgag
      121 aactttttca ctttatatag tgaaagagtt ttctttatgt cctttgaaaa ttgaatagac
      181 caaggtggga ctttatttat ttgtcgagca gctgaataag atacgcgatg atatgagcga
      241 gaaactatct agttcaaaaa ttgactggaa accaatttgc catgacacaa ataatgttaa
      301 tgatacttct gaacgttcag gctctagtcg aaaaatggag caggtgagat tcctatgttg
      361 atttccaaat caacccacca atgtcgtgaa acttagttcg gagtaacatg cattcgaatc
      421 gtttcggata gatagaggta ttctatgaag acaataactc acaaagaatt aatgaaattg
      481 gggtttgcaa aaactacagc tagaaaaata atacgacaag caaaaaatat tgcaattcag
      541 agatttgaag tagcagacaa aaatagtgta aatgcggtaa aattaagtaa atccccattc
      601 gacaataggc gattagactt agcaccgtta tctattgttg aagaattact aggtttctcg
      661 ctcgtggaat acgaggagaa gaac
//
LOCUS       DASFSW010000311          682 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.315, whole genome shotgun sequence.
ACCESSION   DASFSW010000311 DASFSW010000000
VERSION     DASFSW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.315"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>682)
                     /locus_tag="U1099_002279"
     CDS             complement(<1..>682)
                     /locus_tag="U1099_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEM4868540.1"
                     /translation="NQPPLERLAYLLGEGQNQVLNPLWNGNFTGKSVLGFCFGFVTMG
                     LVYLYVSTGQKVYREGAEYGSARFGNSHERKAFLSKNPFNDTILSRNVRLTLLEKKAL
                     QFDRNKNLVVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKTGKLFLEHGYQV
                     KVLDLVNMTNSDGFNPFRYVETENDLNRMLTVYFNNTKGNGSRSDPFWDEASMTLVRA
                     ISSYLVDFY"
BASE COUNT          201 a          158 c          142 g          181 t
ORIGIN      
        1 tgtaaaaatc caccaagtaa gaggaaatag cacggactaa ggtcatggac gcctcatccc
       61 aaaagggatc actgcgactg ccattgcctt tggtattatt gaaatagacc gtaagcatac
      121 ggttcaaatc attctccgtt tccacataac gaaaaggatt aaagccatct gagtttgtca
      181 tgtttacaag gtctaagacc ttgacctgat agccatgctc taaaaagagc ttccctgtct
      241 tttcagctaa gtgatcctta gggtcaacga caatattgga acagttgagc tgaatcagat
      301 tgggtttcac aaagcgaaag gtctttcccg cccctgaccc accaatgacc acaaggttct
      361 tgtttcggtc aaactgtagg gctttctttt ctaacagggt tagcctaacg tttcgtgaca
      421 aaatagtgtc attgaaagga ttcttactga gaaaggcttt gcgttcgtga ctgtttccaa
      481 aacgggcaga gccatactcc gccccttctc ggtaaacctt ttgaccagtt gaaacataaa
      541 gatagactag gcccatcgtc acaaatccaa agcaaaagcc caaaactgat ttacctgtaa
      601 agttgccatt ccacaaggga tttaagacct gattctgtcc ctctcctaag agataggcaa
      661 ggcgttccaa gggtggttga tt
//
LOCUS       DASFSW010000312          679 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.316, whole genome shotgun sequence.
ACCESSION   DASFSW010000312 DASFSW010000000
VERSION     DASFSW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.316"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..674
                     /locus_tag="U1099_002280"
     CDS             <1..674
                     /locus_tag="U1099_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mac family protein"
                     /protein_id="HEM4868541.1"
                     /translation="STNVEAASVDNSEIHADTSLTAVSSVNLDNPVIEPVAIPLIGSK
                     RDTNAEVEVSSLSKREVRKPNTEGLISVQSKVIKKELLESSLVEAGSPLLEATIAQSS
                     NSNSTEIGMSYQNTVLLESNNTERQVSKAEIVIEHKETELVETVSSASEPVVLVENIS
                     QTSNNTIESGKNMGVQSQAGAKQILGIEQSSKVSTPTSRQIMGVGLLTLVLGSALGLL
                     KKRRK"
BASE COUNT          233 a          118 c          139 g          189 t
ORIGIN      
        1 aatcaactaa tgtcgaagct gcatctgtcg acaattctga aattcatgca gatacctctc
       61 taactgctgt ttcatcagtc aatctggata atccagtgat tgaaccagta gctatccccc
      121 ttatcggttc taagagggac acgaatgcag aagtggaagt ttcttcatta tcgaaaagag
      181 aggttagaaa accaaatact gaagggctaa tctctgttca atcaaaagtt attaagaaag
      241 aattgctaga atcaagctta gtagaagcag ggtctccatt gctagaagcc accattgctc
      301 agtcttcaaa ctcaaatagt actgagatag gtatgagcta tcagaatact gtgttattag
      361 agtctaataa tacagagcgt caggtgtcta aagcagaaat tgttatagaa cacaaggaga
      421 cagagttagt tgaaacggtt tcatctgctt ctgagcctgt agtgctcgta gaaaatatct
      481 cacaaacctc aaataatact attgaatctg gtaagaatat gggagttcaa tctcaagcag
      541 gtgcaaaaca aattttaggc atagaacaat cttcgaaagt aagtacacct acttcaagac
      601 agattatggg agtcggtcta ttgactcttg ttcttggtag tgctttaggt ttgttaaaga
      661 aaagacgtaa gtaaaagaa
//
LOCUS       DASFSW010000313          663 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.317, whole genome shotgun sequence.
ACCESSION   DASFSW010000313 DASFSW010000000
VERSION     DASFSW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.317"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(38..>663)
                     /locus_tag="U1099_002281"
     CDS             complement(38..>663)
                     /locus_tag="U1099_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM4868542.1"
                     /translation="KARLLIGLGGLAVTSFMVMMGYTIGSQSVTNHTQKQIQSEAQKL
                     LTKEKEKEKASVLSDELVKEFLTQYYTKEKLGENNNRIKPYMTDSAFKEEVARQEDSI
                     NQVYKDYMLDYRFESAQIYVNTEANEALAEVSYQVIYVSDISDKAQKSTQTETTSLKL
                     SYAKLSDKLLVNQVTIWNGKLGDLKESSDEVNSSIPKIQGTTTSETN"
BASE COUNT          175 a          143 c          126 g          219 t
ORIGIN      
        1 tcatctcctt atcaaaaaag cagaagatat actcctgcta gttggtctca cttgtcgtag
       61 tgccttgaat ctttggaatg cttgaattta cttcatcaga agattctttc aagtccccta
      121 gcttcccatt ccaaatggtt acctgattga caaggagttt atctgacaat ttggcataag
      181 ataatttaag agaggtcgtt tctgtctgag tgcttttctg agccttatcg ctgatatcag
      241 acacatagat aacttgataa gacacttccg caagagcttc atttgcttct gtattgacat
      301 agatttgagc agactcaaag cgataatcca acatatagtc tttatagacc tggttgatgg
      361 aatcttcttg acgagctacc tcttccttaa aggcagaatc tgtcatatag ggcttgatac
      421 ggttgttgtt ctcgcccagc ttttccttag tatagtattg ggtgaggaat tccttgacta
      481 gctcatcaga gagaacactc gccttctctt tttctttctc tttggtcagt aatttttgag
      541 cctcagactg gatttgtttc tgtgtgtgat tggttacaga ctgagaacca atggtgtaac
      601 ccatcatcac cataaagcta gttacagcta gaccacccaa accaatcaat agccgagcct
      661 tca
//
LOCUS       DASFSW010000314          647 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.318, whole genome shotgun sequence.
ACCESSION   DASFSW010000314 DASFSW010000000
VERSION     DASFSW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.318"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..500)
                     /locus_tag="U1099_002282"
     CDS             complement(<1..500)
                     /locus_tag="U1099_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868543.1"
                     /translation="MLDKLRKSQNEYLELLRRVRGDLDLGDVAYHLDKIRIFWFRKQK
                     LLEVCSQHLFNNLDTYFYTAVSKFNVDNLDKNILFALGSYQIFDDPILSYLEVVEKSN
                     ALHDMKDYITKLKERIIECIDDLIVLLEKEIPNFYVLPLRFSNSLINKEKIDIIPFIE
                     NFFIEG"
BASE COUNT          216 a          110 c           75 g          246 t
ORIGIN      
        1 ataccttcta taaagaaatt ttcaataaat ggtatgatat caattttttc tttgttgata
       61 agtgaattag aaaatcttaa tggtaataca taaaaatttg gaatttcttt ttctaataat
      121 actattaaat cgtcaatgca ctctataatc ctttctttta attttgtgat gtaatctttc
      181 atatcatgca atgcattact tttttctacc acctccaagt aggatagtat tggatcatca
      241 aatatttgat aacttcccaa tgcaaaaaga atgttcttat ctaagttatc aacgttaaat
      301 tttgatactg cagtgtaaaa atatgtatcc aagttattaa aaaggtgttg actacacact
      361 tctagtaatt tttgttttct aaaccaaaaa attctaattt tatctaaatg ataagctaca
      421 tctcctaagt ctaagtcgcc tctaactctc ctcagcaact ctaaatactc attttgtgat
      481 ttcctaagtt tatctagcat aatctatcac catctcttcg ctcacttgga ataggaaata
      541 cttcgttaat gacagataaa tgctttgtta atatcattac catgtcatga ctaatgtcag
      601 gtaattcctg tacatcatct atatcaggaa ttccatgaaa ttgatgc
//
LOCUS       DASFSW010000315          640 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.319, whole genome shotgun sequence.
ACCESSION   DASFSW010000315 DASFSW010000000
VERSION     DASFSW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.319"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>640
                     /locus_tag="U1099_002283"
     CDS             <1..>640
                     /locus_tag="U1099_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucan-binding protein"
                     /protein_id="HEM4868544.1"
                     /translation="QEKAEKHVFRKSKKYRTLCSVALGTMVTAVVAWGGQVAQADEVV
                     TPSLDKTVQLTENPATNLPEAQPTPVGEQTNSLASTGQSDGAITVTVPHDVVTQAVNQ
                     ATAEGVTTVQDQPMDLGNTTSASETGKQLDKAEADAAKQAEAITQVTNTYKADKATYE
                     QDKTRVEQGNAALAASHKEATLAGKALNSSVDSTASKIKDQDPSANVTVTTQT"
BASE COUNT          210 a          142 c          150 g          138 t
ORIGIN      
        1 atcaggaaaa agcagaaaaa cacgtcttcc gtaaaagtaa gaagtaccgt acgctttgtt
       61 cggtagcact tggaactatg gtgacggctg ttgtcgcttg gggtggccaa gtagcacaag
      121 ctgacgaagt ggtaacacct tcgctagata aaaccgttca gttgacagaa aatcctgcga
      181 caaatttacc agaagctcag ccaactccag ttggtgaaca aacgaatagt cttgcctcaa
      241 ctggtcaatc agatggggct attacagtca ccgttcctca tgatgttgtg acacaggctg
      301 tcaatcaagc aactgctgaa ggagttacta ctgttcaaga ccaacctatg gacttgggaa
      361 atacaacttc tgcgagtgag accggtaagc aattagataa agcggaagcg gatgctgcta
      421 agcaggctga agccatcact caggtgacca atacctataa agcagataaa gccacctacg
      481 aacaagataa aactcgtgtt gaacaaggga atgctgcgtt ggctgctagt cataaagaag
      541 ctactctagc aggaaaggcc ttgaatagtt cagtagatag cacagcttca aaaatcaaag
      601 accaagatcc gtcggctaat gtgaccgtca caactcaaac
//
LOCUS       DASFSW010000316          620 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.320, whole genome shotgun sequence.
ACCESSION   DASFSW010000316 DASFSW010000000
VERSION     DASFSW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.320"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>620
                     /locus_tag="U1099_002284"
     CDS             <1..>620
                     /locus_tag="U1099_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEM4868545.1"
                     /translation="GQWTDTADGSRRHTLLLEYAKQKNQKWYDLGLQLDFMLHGDNPY
                     YTNWLKDFFKNSGSPASLAQVFLIYWEGNSGDKLLERQTRATEWYYQIEKGFSQPNGG
                     TAQSDPKALEAVRGDLYDNSVPGGGDGMGYAYGQCTWGVAARINQLGLKLKGRNGEKI
                     PIISTMGNGQDWVATAARLGGDTGKLPKAGAIISFAGGGHGSPTEYG"
BASE COUNT          189 a          122 c          167 g          142 t
ORIGIN      
        1 gggcagtgga ctgatactgc agatggatca cgtagacata ccttattatt agagtatgcc
       61 aaacaaaaaa accagaagtg gtatgactta ggcttacaac tggatttcat gttgcatggg
      121 gacaatcctt actataccaa ctggttaaaa gactttttca aaaactcagg aagcccagcc
      181 agtcttgccc aagtctttct catttattgg gaaggaaata gtggtgataa gctcttagaa
      241 cgtcaaacga gagcaacgga gtggtattac caaattgaga aaggctttag ccaaccaaat
      301 ggtggaaccg cccagagtga tccaaaagcg ctggaagcgg ttcgaggtga cctttatgac
      361 aactccgttc caggtggagg tgacggtatg ggctatgcct atgggcaatg tacttgggga
      421 gttgccgctc ggattaacca attgggcttg aaactcaaag gccgaaatgg tgagaaaatc
      481 ccaatcatta gtaccatggg gaatggtcaa gattgggtgg caacagcagc acgtcttggt
      541 ggagatacag gaaagctacc aaaagcaggg gcaattatct cctttgcggg aggaggtcat
      601 ggatcgccaa cagaatacgg
//
LOCUS       DASFSW010000317          619 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.321, whole genome shotgun sequence.
ACCESSION   DASFSW010000317 DASFSW010000000
VERSION     DASFSW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.321"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..491
                     /locus_tag="U1099_002285"
     CDS             <1..491
                     /locus_tag="U1099_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868546.1"
                     /translation="LNAIMQEQTKPILLVLKNLDDYLTYDSFVRIAQYLEELSNKYPY
                     FNTILFPSQEGYLYLTEATLETVNIVSDRIEHYPAFTFLYTRYQQSYPSTSPLGEKEF
                     LNSLRKISSYLFSSDINRVVSLADIDLVTLKIVNSLYQYDIKMVYMYKGISKLEENYL
                     SS"
BASE COUNT          214 a          103 c           99 g          203 t
ORIGIN      
        1 tgttgaatgc aataatgcaa gaacaaacaa aaccaatttt actggtactt aaaaatttag
       61 atgattacct aacttacgat tcttttgtga gaatagcaca gtatttagag gaacttagta
      121 ataagtatcc ctatttcaac acaatcttgt ttccttctca agagggctat ttgtacttga
      181 ccgaggcaac gcttgagact gttaatattg tgtctgatag aattgaacac taccctgctt
      241 tcacgtttct ctatactcgt tatcagcagt catatccctc aactagccca ctaggtgaaa
      301 aagaattttt aaactctttg agaaaaatca gttcctattt attctcttca gacattaata
      361 gagtcgtgag cttagcagac atagacctag taactctaaa gatagttaat tcactttatc
      421 agtatgatat taagatggta tatatgtata aaggaatatc aaaattagag gaaaattacc
      481 tttcttccta aatttgacaa gtttttcgat aaatattata attacggaag agagtacaaa
      541 aaccatttga ggtttttgta ctctcaagat ttaagtaaca gtaaaactgg ccaaacgtgg
      601 gatcaaaacc atgttggtt
//
LOCUS       DASFSW010000318          602 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.322, whole genome shotgun sequence.
ACCESSION   DASFSW010000318 DASFSW010000000
VERSION     DASFSW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.322"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>602
                     /locus_tag="U1099_002286"
     CDS             <1..>602
                     /locus_tag="U1099_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM4868547.1"
                     /translation="SVYDESLEAYNQYRLDNKYLTKIIAEKNPDLIIPLDADEFLTAD
                     SNPRKLLEQLDLEKIHYVNWQWFVMTKKDDINESFIPRRMQYCFEKPVWHHSDGKPVT
                     KCIISAKYYKKMNLKLSMGHHTVFGNPNVRIEHHNDLKFAHYRAISQEQLIYKTICYT
                     IRDIATMENNIETAQRTNQMALIESGVDMWETAREASYSG"
BASE COUNT          220 a           89 c          111 g          182 t
ORIGIN      
        1 tatccgtata tgatgagtct ttagaggcat ataatcagta tcgacttgat aataaatatc
       61 taacgaaaat aattgctgaa aaaaatccag atttgataat acctttggat gcggatgaat
      121 ttttaacagc cgattcaaat ccacggaaac ttttggaaca actggactta gaaaagatac
      181 attatgtgaa ttggcaatgg tttgttatga ctaaaaaaga tgatattaat gaatcgttta
      241 taccacgtag aatgcaatat tgttttgaaa aacctgtttg gcatcattct gatggtaaac
      301 cagttactaa atgtataatt tccgctaagt attacaaaaa aatgaattta aagctatcga
      361 tgggacatca cactgttttt ggtaacccaa atgtaaggat agaacatcat aatgatttga
      421 aatttgcaca ttatcgagct attagccaag agcaattaat ttataaaaca atttgttaca
      481 ctattcgcga tattgctact atggagaaca atatcgaaac agctcaaaga acaaatcaga
      541 tggcgctcat tgaatctggc gtggatatgt gggaaacggc gagagaagcc tcttattcag
      601 gt
//
LOCUS       DASFSW010000319          600 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.323, whole genome shotgun sequence.
ACCESSION   DASFSW010000319 DASFSW010000000
VERSION     DASFSW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.323"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(61..>600)
                     /locus_tag="U1099_002287"
     CDS             complement(61..>600)
                     /locus_tag="U1099_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEM4868548.1"
                     /translation="EIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILG
                     NCDSLLYLGGNDEETFKFMSGLLGKQTIDVRSTSRSYGQTGSGSTSHQKIARDLMTPD
                     EVGNMKRDECLVRIAGVPVFKEKKFFPLKHKNWKYLADKESDERWWHYHIDPLKTEDV
                     PFEPSAHKVRDLSKETTLH"
BASE COUNT          142 a          141 c          123 g          194 t
ORIGIN      
        1 tagcgtccac gccataaaca aagcctgtta atttttggtt catacgattc ctcttttcta
       61 ttagtgtagt gtggtttctt tgcttaaatc cctaaccttg tgggctgagg gttcaaacgg
      121 aacgtcctct gttttgaggg ggtcaatgtg gtaatgccac caacgttcat ctgactcctt
      181 atccgctaag tatttccagt tcttgtgttt caaggggaag aactttttct ccttaaaaac
      241 aggaacccct gcaattctca ccaagcattc atcacgtttc atgtttccga cttcatcagg
      301 ggtcatgaga tcccttgcga ttttctgatg agacgttgac ccagaccctg tttggccata
      361 cgaccgactg gtacttctca catcaatggt ttgtttgcct aagagaccac tcataaactt
      421 gaaggtctct tcatcattgc ctccaagata aagcaagcta tcacagtttc ctagaatagt
      481 tttccaagcc tccttctcct tatagagccc ttgaagttgg gcaatgtttt gaagaatggg
      541 tacgagactc atgttccgtg agcgaactgt tgaggtttgt tccgcaaagt ctggaatttc
//
LOCUS       DASFSW010000320          589 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.324, whole genome shotgun sequence.
ACCESSION   DASFSW010000320 DASFSW010000000
VERSION     DASFSW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.324"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>589
                     /locus_tag="U1099_002288"
     CDS             <1..>589
                     /locus_tag="U1099_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISSsu6 family transposase"
                     /protein_id="HEM4868549.1"
                     /translation="VKKNHQISVAVNQKIAQLLIENQAMTHIAHRLSISTSSVMRKLN
                     EFKFETDWNTLPEVMSWDEYSFKKGKMSFIAQDFNSLNVITILDGRTQATIRNHFLRY
                     PRKVRNQVKVITMDMFSPYYQLAKQLFPNAKIVLDRFHIVQHLSRAMNRIRIQIMNQF
                     DRTSQEYRALKRYWKLIQQDSRKLSDKRFYRPMFR"
BASE COUNT          199 a          127 c          102 g          161 t
ORIGIN      
        1 ttgtcaagaa gaaccaccaa atctctgtcg cggttaacca gaaaatcgcc caattactca
       61 tcgaaaatca agcaatgaca catattgcac acaggctatc tatctcaacc tcatcagtta
      121 tgagaaagct taatgagttc aagtttgaaa cagattggaa taccttacct gaggtgatga
      181 gctgggatga atatagcttc aagaagggga aaatgagctt tatcgctcaa gatttcaact
      241 ccctaaatgt cattactatt ctggacggaa gaacacaagc aaccatccga aaccactttc
      301 tacgctatcc tagaaaggtt agaaatcaag tcaaagtcat taccatggat atgtttagtc
      361 cctattatca acttgctaaa cagctttttc cgaatgcaaa aattgtactc gaccgctttc
      421 atattgttca acaccttagt cgtgctatga accgtattcg tattcaaatt atgaatcaat
      481 tcgacagaac atcgcaggaa tacagagcct tgaaacgcta ctggaaattg attcaacagg
      541 atagtcgtaa actcagcgat aaacgatttt atcgccctat gtttcgaat
//
LOCUS       DASFSW010000321          577 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.325, whole genome shotgun sequence.
ACCESSION   DASFSW010000321 DASFSW010000000
VERSION     DASFSW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.325"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>577
                     /locus_tag="U1099_002289"
     CDS             <1..>577
                     /locus_tag="U1099_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868550.1"
                     /translation="QLTDCEVYSIVMDRSNAIDLNEIKGTVYSALKYYRDMLQNIEPF
                     NEKLYVLTSILTYSYKRLLELMNHDLQYTISGRSIVRSCIENYVMTKYLIAEEANHKN
                     IWEEYQYYGIGGYKLVSERFIENQPELTNPHINFEYLNLLVSEFQNKEFIDMDTRYFG
                     NGNIRQKFKQVGEDDLYKFYYDYDSQFEHGLW"
BASE COUNT          213 a           66 c          106 g          192 t
ORIGIN      
        1 caactaactg attgtgaggt atattcaatt gttatggata gaagtaatgc tatagatttg
       61 aatgagataa aaggtacagt atattcagca ttaaaatatt acagagacat gcttcaaaat
      121 atagagcctt ttaacgagaa gctttatgtt ttaactagta tcctaaccta ttcttataag
      181 cggttactag agttgatgaa tcatgatttg caatatacga tttcagggcg ttctatagta
      241 aggtcatgca ttgagaacta tgttatgaca aaatatttaa ttgctgagga agcaaaccat
      301 aaaaacattt gggaggaata ccaatattat ggtattggtg gttataaatt agtttccgaa
      361 cgctttatag agaatcaacc agagttaaca aatcctcata ttaattttga atatttaaat
      421 ttgctagtgt cggaatttca aaataaagaa tttatagata tggacactag atattttgga
      481 aatggcaata taagacaaaa atttaagcaa gtaggggaag acgatttata taaattttac
      541 tatgattatg attctcaatt tgaacatgga ctttggg
//
LOCUS       DASFSW010000322          577 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.326, whole genome shotgun sequence.
ACCESSION   DASFSW010000322 DASFSW010000000
VERSION     DASFSW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.326"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..187
                     /locus_tag="U1099_002290"
     CDS             <1..187
                     /locus_tag="U1099_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868551.1"
                     /translation="SGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAI
                     GLVSIMDNKLTILPQIQ"
     gene            217..>577
                     /locus_tag="U1099_002291"
     CDS             217..>577
                     /locus_tag="U1099_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEM4868552.1"
                     /translation="MNQSNEIFLNTIQGKTHYKRPISSAFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVISSLRS"
BASE COUNT          198 a           65 c          122 g          192 t
ORIGIN      
        1 ttcaggacaa aagaaattaa ttgaacaact ttatagatat ttgtgtgtaa ttgaagaagg
       61 atttgttatt gcgggagata atggtttaaa gataacaaca gatattgctt caggatttgc
      121 gggtgttgca attggattgg tatcaataat ggataataaa ttgacgattc ttcctcaaat
      181 acaatagaga gatgccgttg ttaaggggtt atacttgtga atcagagtaa tgaaatattt
      241 ttaaatacaa tacagggaaa aacacactat aagaggccta tctcttcagc atttcaggaa
      301 ttaaaaaaat ggaattcttt gatatttagg agagggcgat attggtacca atttgataat
      361 tggagtatac cgaatactgg tgtaataaag gaatggtatg tcgaagataa tgactggatt
      421 cctaaggtta gttcttccag tttagataat tcaaattcac ttaaaattgt ctttgatgtg
      481 gaaaaatata ctttaccaga tgatagtatc ttacaggatg ttaggatttc atctgatgag
      541 agttatatag ttttggttat atcctcgctg cgtagca
//
LOCUS       DASFSW010000323          572 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.327, whole genome shotgun sequence.
ACCESSION   DASFSW010000323 DASFSW010000000
VERSION     DASFSW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.327"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            5..>572
                     /locus_tag="U1099_002292"
     CDS             5..>572
                     /locus_tag="U1099_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM4868553.1"
                     /translation="MEVIIQNLRKSYKDKEVLKDVSFKIEEGTICGLLGVNGAGKSTI
                     MKILFGLERADSGEVIFNGKHKDIVEENRFDIGALIESPAIYLNLSAFDNLKTRALLH
                     DIPDERIYEVLQLVGLSDTGKKKAGHFSLGMKQRLGLGMAIITNPDLLILDEPTNGLD
                     PDGIKELLNLIRDLKKSGMTVLLSSHQLS"
BASE COUNT          204 a           72 c          121 g          175 t
ORIGIN      
        1 aaaaatggaa gttatcattc aaaatctaag aaaaagctat aaagacaaag aggttttaaa
       61 agacgtttca tttaaaattg aagaaggtac tatttgtggt cttctgggag taaatggggc
      121 tgggaaatcc accattatga aaattctttt tgggttagaa agagctgata gtggagaagt
      181 cattttcaat ggaaagcata aggatattgt agaagaaaat agatttgata ttggagcgtt
      241 gatagaatct cctgctatct atttgaatct ttctgcattt gacaatttaa aaacacgagc
      301 attgctacat gatattccag atgaaagaat ttatgaggta cttcagttgg ttggtttatc
      361 tgatactgga aaaaagaagg cagggcactt ttcattagga atgaagcaac gacttggttt
      421 aggaatggca attattacca atcctgattt attaatttta gatgagccaa ctaatggtct
      481 agatccagat gggataaaag aattgctaaa tttaattagg gatctaaaaa aatctggaat
      541 gacagtcctc ttgtccagcc atcaactaag tg
//
LOCUS       DASFSW010000324          563 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.328, whole genome shotgun sequence.
ACCESSION   DASFSW010000324 DASFSW010000000
VERSION     DASFSW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.328"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(7..321)
                     /locus_tag="U1099_002293"
     CDS             complement(7..321)
                     /locus_tag="U1099_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868554.1"
                     /translation="MKQLVTKRSYKIGLSAMALVLLMTTAGGATAHASTGTYSVETRG
                     DAEVYRLLETPAGSFTQGNVVLEPVNNSTTKPSETKSWFDSFWSSVKTFWEAICRGVG
                     WC"
BASE COUNT          200 a          118 c           80 g          165 t
ORIGIN      
        1 ttctacttaa caccatccaa ctccacgaca gattgcttcc caaaatgttt ttacactact
       61 ccaaaaagaa tcaaaccatg atttggtttc agatggttta gttgtagaat tattcacagg
      121 ttccaaaact acgttcccct gtgtaaaaga ccccgccgga gtttccaaca gcctatacac
      181 ttcggcatca cctcgagttt cgacactata ggtacccgta cttgcatgtg cagtggcacc
      241 acctgcagtg gtcattagca agactaaagc catagcactc aaaccaattt tataactacg
      301 ttttgttaca agttgtttca ttgttgaaat atctccttta tacgatattc gtctctacaa
      361 atgatagtat agcacaaaca gacaactcaa attccaactt tagaaaaagg ttagataaaa
      421 aactataaaa ctccaagata tgcatatatt aatacaagaa caaaaataca catgcaatat
      481 tatatttatg gttcaaaaaa cttgtatcga ataattgtgg taataaagaa aaaatgttta
      541 aaaccctcta ttttcataga ggg
//
LOCUS       DASFSW010000325          562 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.329, whole genome shotgun sequence.
ACCESSION   DASFSW010000325 DASFSW010000000
VERSION     DASFSW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.329"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
BASE COUNT          209 a          107 c           80 g          166 t
ORIGIN      
        1 ataatattca ttttccttga ataaaataag gacaagattt acaataagat tatgaatacc
       61 ttaatagaat atatcgatcc gattatcctc atagtactaa taatctctta tagcatctat
      121 cgcagatgcc atcctaataa tatggacact aaacaaacgc tcaaatgtcc aaaatgtaag
      181 tcaataaaac tgcaaactta cgttccaaca caaaaaagat attctcgtaa agccggtatg
      241 attggtcatg caatcaatgg catagaggga gctatgtttg caatgctcta tgaagctgat
      301 cggacttatt atcgttgtca acattgcagt taccgatttt ctagaaagga gaatcgtttt
      361 atttctaaca tcgaataacc tccctgattt ttagagaatt tcgctattga aaacgaagaa
      421 ttctcacccc ttacaaatcc cgttatatca acatttacga aaatgaaact tccatgaaaa
      481 tggactgaaa tcctctgatt tggataaaat cgacaccaat taacaccata aaatagacta
      541 gtcatcagta ggtcaaacta at
//
LOCUS       DASFSW010000326          554 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.330, whole genome shotgun sequence.
ACCESSION   DASFSW010000326 DASFSW010000000
VERSION     DASFSW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.330"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            1..527
                     /locus_tag="U1099_002294"
                     /pseudo
     CDS             1..527
                     /locus_tag="U1099_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          167 a           94 c          128 g          165 t
ORIGIN      
        1 tggaacaatt caatcaagaa cagttatatg gagtggttat tcaaaccatg acagctgcta
       61 gtcaacttcc tatggttaaa gtcaatcggg aagaattttt gaagactcgt tttaaagata
      121 gtccatatct agataaaatt attgcggatg gaccaacagc ggtcttcact acaaaagctt
      181 tgcgtaagga agctgaaaaa atcattaatg atatgactac gaaaacatct ttgatttctt
      241 tcgcagctgg tttaccgggg aatccattta cagcggtggc ggcaggtact gctgatatag
      301 ctcagtattt tggttttgcc ttgaatttgt cgcaacaaat cgcttatctt tttggtgaag
      361 atgaattatt tgttggtgaa gggaaggaaa tttcagaaga ggttcaaatc cgcattattg
      421 cctacattgg tgtcatgttt ggagcaggtg gttcagcggt tcttctcaat aaagtatcca
      481 agacagtcgg agctaatatc ggtaaaaaag tagctatgca ggctttgtag gctgggcaaa
      541 aagaccaacc tcgc
//
LOCUS       DASFSW010000327          545 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.331, whole genome shotgun sequence.
ACCESSION   DASFSW010000327 DASFSW010000000
VERSION     DASFSW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.331"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>545
                     /locus_tag="U1099_002295"
     CDS             <1..>545
                     /locus_tag="U1099_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEM4868555.1"
                     /translation="PLRGENSSASLERLGNVNPSGKGMNGEVYLSRGLAPTLTRGKGE
                     GTKIAIPVLTPDRLEKRQHGRRFKGNDDPMFTLTSQDRHGVVVAGTLPTSFIQTGRVY
                     DLSGLSPTLTTMQGGDKVPKILCREEAPHLKIRKATKLGYAKAIVGDSVNLAYPESNK
                     RRGRVGKGISNTLTTSDNMGVV"
BASE COUNT          169 a          112 c          133 g          131 t
ORIGIN      
        1 cctctcagag gagaaaacag ctcagctagt cttgaacgat taggcaatgt taatccgtca
       61 ggcaaaggca tgaatgggga agtctattta tcacgtgggc tagcaccaac cttaaccaga
      121 ggaaaaggtg agggaactaa aattgccatt cctgtcttaa cccctgatag gctagaaaaa
      181 cgccaacatg ggagacgctt taaagggaac gatgatccga tgtttacttt aactagccaa
      241 gatcggcatg gtgtagttgt cgcaggaact ttaccaactt cttttatcca aactggacgt
      301 gtctatgacc tttcagggct ttctccaacg ttgacaacca tgcaaggagg ggataaagtt
      361 cctaagattc tctgtcgtga agaagcgcca catttgaaaa ttaggaaagc gactaaacta
      421 gggtatgcca aggcaatagt gggagattct gtcaaccttg cttacccaga gtctaacaaa
      481 cgcagaggac gagtgggaaa ggggatttca aatactttaa ccacttctga caatatggga
      541 gtggt
//
LOCUS       DASFSW010000328          544 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.332, whole genome shotgun sequence.
ACCESSION   DASFSW010000328 DASFSW010000000
VERSION     DASFSW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.332"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            35..>544
                     /locus_tag="U1099_002296"
     CDS             35..>544
                     /locus_tag="U1099_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM4868556.1"
                     /translation="MSRDKRLKEITNILHSRKRIDVKQLEKLTFSSTSTLRRDLIYLE
                     EQGLIKRKHGEVVLNAFNTVELAHSIRETENRSEKKIIAELAKDFVGPGMCIYLDSST
                     TVYELCPFLSTIDNLIIFTNGLNTAQLLSELSNPTMKIFVTGGEVKHHSASVVNHSME
                     NSLLEHFSID"
BASE COUNT          196 a           85 c           98 g          165 t
ORIGIN      
        1 agaatatgtt attatgtact taagaggtgt tagtatgagt agagataaac gattaaaaga
       61 aataacaaat attcttcaca gtagaaagag aatagatgta aaacaacttg aaaaattaac
      121 tttctcaagt acatcaactc ttcgtagaga tttaatctat cttgaagaac aaggtctcat
      181 caaaagaaaa catggggaag tggtgctaaa cgcatttaat accgtcgaac ttgctcatag
      241 tatcagagag actgaaaata ggtcagaaaa gaaaatcatt gctgagcttg caaaagattt
      301 tgttggtcca gggatgtgta tttacttaga ttcaagtaca acggtttatg aattatgtcc
      361 gttcctttca acaattgaca atctaattat ctttacgaat ggactcaata ctgctcagtt
      421 gctttctgaa ctttctaatc caacaatgaa aatatttgtc actggcggtg aggtcaaaca
      481 ccattctgcc tcagtcgtaa accatagtat ggaaaatagt cttcttgaac attttagtat
      541 agac
//
LOCUS       DASFSW010000329          540 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.333, whole genome shotgun sequence.
ACCESSION   DASFSW010000329 DASFSW010000000
VERSION     DASFSW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.333"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..531)
                     /locus_tag="U1099_002297"
     CDS             complement(<1..531)
                     /locus_tag="U1099_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM4868557.1"
                     /translation="MKVSHGYVGMSFDIGKPKRLIFCESVIDMMSYYQLYQKQLSDVR
                     LVSMEGLKLSVIAYQTLRLAAEEQGKLEFLDTVKPSRLTHYLHAIQETTTFFQTHPGL
                     LTLAVDNDEAGRDFCQKLSDKGLPIEADLPPLQELETKADWNDIVKYQNNHSLKDVIQ
                     SAKLQVIRGKPPPRRTT"
BASE COUNT          145 a          111 c          103 g          181 t
ORIGIN      
        1 ggttgttctt ctaggcggag gtttgcctct gataacttgt aacttggccg attggatcac
       61 atcttttaga gaatgattat tttgatattt tacaatatca ttccaatctg ctttcgtttc
      121 cagttcctgt aatggtggta agtctgcttc aatgggaagt cctttgtcag ataatttctg
      181 acaaaagtct cgccctgctt catcattatc aacagctaag gttaataacc ctgggtgcgt
      241 ctgaaagaag gtagtcgtct cttgtatagc atgaaggtaa tgcgttaatc gacttggttt
      301 gaccgtatct aaaaactcaa gtttgccttg ttcctcagct gctaatcgta aggtctgata
      361 cgcaatcaca gagagtttta ggccttccat agaaaccaaa cgcacatctg ataactgctt
      421 ctgatagagt tgataatagc tcatcatatc aatcaccgat tcacaaaaga taaggcgttt
      481 cggttttcct atgtcaaaac tcattcccac atatccatga gagactttca tgattttctt
//
LOCUS       DASFSW010000330          534 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.334, whole genome shotgun sequence.
ACCESSION   DASFSW010000330 DASFSW010000000
VERSION     DASFSW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.334"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..332
                     /locus_tag="U1099_002298"
     CDS             <1..332
                     /locus_tag="U1099_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868558.1"
                     /translation="NRLSPHLYKVFKLLPLGMDLPITGADMERLTGLDVRTIREHIRQ
                     LIVDYGIPVCGGRDNKLGGYYIPQNEVERLAGVLPLQRQYDQEHKRIHALLTADLQDW
                     RKYRDEA"
     gene            322..>534
                     /locus_tag="U1099_002299"
     CDS             322..>534
                     /locus_tag="U1099_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM4868559.1"
                     /translation="MKLELTAQSEADLKTGILELIENYLEAREQTPPRLLGLITAQQV
                     KDELGIKDKTLKRWEDNGLRRYQPPLE"
BASE COUNT          176 a          124 c          130 g          104 t
ORIGIN      
        1 aaaatcgact atcaccacac ctatacaaag ttttcaagtt actaccgctt ggaatggact
       61 tgcctatcac aggggcggac atggaacgac tgacaggctt ggacgtccga accattaggg
      121 aacatatccg ccagctaata gttgactacg gtattcccgt ttgtggtgga cgagataaca
      181 agctaggggg ctactatatc ccccagaatg aagtagaacg acttgcagga gtgctaccgc
      241 ttcaacgaca atatgaccaa gaacacaagc gtattcacgc gctactgacc gcagacttgc
      301 aagactggag gaagtacaga gatgaagctt gaattaaccg cacagagtga agcagaccta
      361 aaaacgggca ttctggagct tatagagaac tatctggaag cgcgtgagca aacaccgcca
      421 agactgttag ggctaatcac cgcccaacag gttaaagatg aactaggcat aaaagataag
      481 accttgaaac gttgggaaga taatgggcta agacgttacc agcccccact agaa
//
LOCUS       DASFSW010000331          531 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.335, whole genome shotgun sequence.
ACCESSION   DASFSW010000331 DASFSW010000000
VERSION     DASFSW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.335"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..470)
                     /locus_tag="U1099_002300"
     CDS             complement(<1..470)
                     /locus_tag="U1099_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM4868560.1"
                     /translation="MYYGRDTEIEELKQHFKFSSNDKNSLYIINGLSGSGKTELIKEV
                     CQQVYSHDKEAFILYIDVTNDGFESNKFFENLLKGAYVPTQFRRNLCLSIPQKYSFSN
                     YLRKQKLTNLTLKHSFRLIRSLISFDTTYKKVIDYPWEEVVTEIKEGIFESDLY"
BASE COUNT          181 a           83 c           60 g          207 t
ORIGIN      
        1 aaatataaat cactttcaaa tattccctcc tttatttcag taacaacttc ttcccaagga
       61 taatcaatta cttttttata ggttgtatcg aatgaaatca aagatctgat taacctaaaa
      121 ctatgtttca gagttagatt tgttagtttt tgttttctta aataatttga aaatgaatat
      181 ttttgaggta ttgataagca tagatttctt ctgaattgag ttgggacata cgctcctttt
      241 agtagatttt caaaaaattt attactttca aacccgtcat ttgtaacatc aatgtagaga
      301 ataaacgctt ctttatcatg actgtaaact tgttgacaaa cctctttgat taactcggtt
      361 ttaccactac cacttaagcc attaataata tataaactat ttttatcatt actggaaaat
      421 ttaaaatgct gttttaattc ttctatttca gtatcgcgac cgtaatacat aaaacatctc
      481 ctaccgataa caatttatta tcattagtaa attgataaat tatcgtatat a
//
LOCUS       DASFSW010000332          530 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.336, whole genome shotgun sequence.
ACCESSION   DASFSW010000332 DASFSW010000000
VERSION     DASFSW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.336"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            57..>530
                     /locus_tag="U1099_002301"
     CDS             57..>530
                     /locus_tag="U1099_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEM4868561.1"
                     /translation="MEHKYTTNNFLVRNAIIGIHELLECDYNSFLETIRENKIFQEQL
                     FVASRSLYESLQKYYSGDSMKRKKINQLSESVYKYYKRSKERSTPFGLFSETSIGSFS
                     STEKLNLNGKTLKKVLLDSEWLIRLVFKIEKEYSRELAYKINPANYQFGDRVVQLF"
BASE COUNT          206 a           60 c           90 g          174 t
ORIGIN      
        1 atttccaaaa taatatttga agatttataa aaataattta gaagttggag ctgctaatgg
       61 aacataaata tactacaaat aattttttgg ttcgtaatgc aatcattgga atacatgaat
      121 tgttggaatg tgattataat tcatttttag aaactataag agagaataag atattccagg
      181 agcaactatt tgtagccagc aggagtttat atgaatcgct tcaaaaatat tatagtggtg
      241 attcaatgaa aaggaaaaaa atcaaccaat tatcagaatc tgtatataaa tattataagc
      301 gtagtaagga gcgctcaaca ccatttggtt tatttagtga aacttcaata ggttcatttt
      361 cttctacaga gaaattaaac ttgaatggaa aaacattaaa aaaagtactc ttggattcag
      421 agtggttaat acgtctagtt tttaagattg agaaggaata ttctcgagaa ttagcctata
      481 aaataaatcc agccaactat caatttggag atagagttgt tcaattgttt
//
LOCUS       DASFSW010000333          520 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.337, whole genome shotgun sequence.
ACCESSION   DASFSW010000333 DASFSW010000000
VERSION     DASFSW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.337"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(58..513)
                     /locus_tag="U1099_002302"
     CDS             complement(58..513)
                     /locus_tag="U1099_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEM4868562.1"
                     /translation="MADSKAVLNQAVADLSVAHSILHQVHWYMRGRGFMIWHPKMDEY
                     MEEIDGYLDEMSERLITLGGAPFSTLKEFSENSQLKEVPGDYNVTIEEQLVRVVEVFR
                     YLAALFQKGFDVSDEEGDSVTNDIFNVAKASIEKHIWMLQAELGQAPKL"
BASE COUNT          160 a          131 c           87 g          142 t
ORIGIN      
        1 ttcgaagagt attaaataat gcagcaaaaa aagagtcctt ctggaccctt catctattta
       61 caacttaggt gcttgaccaa gttctgcctg cagcatccaa atatgttttt caatactagc
      121 cttagccaca ttgaaaatgt cgttggttac gctgtcacct tcttcatcac tgacatcaaa
      181 ccccttttgg aaaagagcag ccagatagcg gaacacctcg accacacgca ccaattgctc
      241 ttcaatcgtt acattgtaat caccaggaac ttccttgagc tgactatttt cactaaactc
      301 tttaagggta gaaaatggtg ccccacctaa ggtgattaaa cgctcactca tctcatccaa
      361 ataaccatca atttcttcca tatattcatc catctttgga tgccaaatca taaagccacg
      421 accacgcata taccagtgaa cttgatggag gattgaatgg gctactgata aatcggccac
      481 cgcttgattc aaaacagcct tagaatcagc cagtgatgga
//
LOCUS       DASFSW010000334          517 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.338, whole genome shotgun sequence.
ACCESSION   DASFSW010000334 DASFSW010000000
VERSION     DASFSW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.338"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>517
                     /locus_tag="U1099_002303"
     CDS             <1..>517
                     /locus_tag="U1099_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEM4868563.1"
                     /translation="EFSLKNSTLTGFYVESKNQMSKTESKLYPLGCLNFGLAHGLAGV
                     GAMLSYSKLKGYSNEKSIAAIKKIIMLYEKHELKNYMWKEGLSDIELKKTEKSNLQYE
                     FIRDAWCYGSPGISLLYLYSSLALEDKKLKSKACNILKASIRRSNGLEQSILCHGFSG
                     AIEICLFFKKIY"
BASE COUNT          189 a           70 c           97 g          161 t
ORIGIN      
        1 gagttttcac taaaaaattc tactttaaca ggcttctatg tggaatcaaa gaatcaaatg
       61 tccaagacag agtctaagct ttatccgtta ggatgtctaa attttggttt agcacacggc
      121 cttgcgggtg ttggcgcaat gttgtcttat tcaaaattaa aaggctattc taatgaaaaa
      181 tctattgcag caatcaaaaa aattataatg ttatacgaaa aacacgagtt aaaaaattat
      241 atgtggaaag agggattaag cgatattgaa ctaaagaaaa cagaaaaaag caatttacag
      301 tatgaattta ttcgagatgc gtggtgctac ggtagcccag gaattagttt actatatctg
      361 tattcaagtt tggctctaga agataagaaa ctaaaatcta aagcttgtaa catcttgaaa
      421 gcatccatca ggagaagtaa tggtttggaa caatctattt tatgtcacgg attttctgga
      481 gcaattgaga tttgcctatt ttttaaaaaa atttata
//
LOCUS       DASFSW010000335          514 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.339, whole genome shotgun sequence.
ACCESSION   DASFSW010000335 DASFSW010000000
VERSION     DASFSW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.339"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..415)
                     /locus_tag="U1099_002304"
     CDS             complement(<1..415)
                     /locus_tag="U1099_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter permease"
                     /protein_id="HEM4868564.1"
                     /translation="MTNIFKSEWLKQKSSSDKKLLILIPLLAIFIAFILAGPLNLESF
                     SIYWWEGGFLFTLLGLLFLADYSAEENAGAFQNISLGRYTFTIYLAKILLKFKDVLIS
                     TFIFTIIFYGISFLFSGVISVDIGKDLICLLLIGVS"
BASE COUNT          206 a           97 c           63 g          148 t
ORIGIN      
        1 aagaaacacc aataagtaat aaacaaatca aatctttacc aatgtctact gatatcacac
       61 ctgaaaaaag aaatgagatg ccataaaata ttatcgtaaa aataaaggtt gaaatcaaaa
      121 catccttgaa cttcaacagt atcttcgcta aataaatagt aaacgtatat ctacccaagc
      181 taatattttg aaacgcacct gcattctcct ccgcgctata atcagctaaa aacaataacc
      241 caaggagagt gaaaaggaat cctccctccc accaataaat tgaaaaactt tctaaattga
      301 gaggtcctgc tagtatgaaa gctataaaaa ttgctagcaa aggaataagg attagtaatt
      361 ttttatcaga actagatttt tgcttcagcc actcagattt aaaaatatta gtcatttcca
      421 tcacctccat ggacaattct aaagaatagt ttttctaaat catttgaatt cttattaata
      481 tcatcgtaaa aaatcttgcc ctgattcagt ataa
//
LOCUS       DASFSW010000336          508 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.340, whole genome shotgun sequence.
ACCESSION   DASFSW010000336 DASFSW010000000
VERSION     DASFSW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.340"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(142..>508)
                     /locus_tag="U1099_002305"
     CDS             complement(142..>508)
                     /locus_tag="U1099_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM4868565.1"
                     /translation="IVNESHTRVIKGNRLNLDRALINILSNATRFCSEEGKVVLTISE
                     SSNFISFEIWNNGLPFTEESLKDGGKLFYTENKGRNDKHYGIGLSFAQKIAMRHQGQL
                     ILSNPQNGGAQVTLLIKKF"
BASE COUNT          160 a           88 c           72 g          188 t
ORIGIN      
        1 tttgttcaga aaaaaattga gcaaaaagtc gatatctctt tgattttttc aaattttcga
       61 aataaaaaat cgcataaaat caacgttttt aaatgaaaga tttcatgcgg ttttgtttat
      121 attaggcttt ttgaccagcc cctaaaattt tttaattaat aaagtgacct gtgcaccacc
      181 attttgtgga tttgagagta ttaattgacc ttgatgtctc atagctatct tttgagcaaa
      241 tgaaagtcct attccgtaat gcttatcatt tcttccttta ttctctgtgt aaaaaagttt
      301 tcctccatct ttaagagact cttctgtaaa tggtaaacca ttattccaaa tctcaaaact
      361 gataaagttg gaactttcag atatagtcag taccaccttg ccctcttctg aacaaaatct
      421 agtcgcatta gataagatat ttatcaaagc tcgatctaag ttgagacgat ttcctttaat
      481 cactctggta tgggattcat taactatt
//
LOCUS       DASFSW010000337          506 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.341, whole genome shotgun sequence.
ACCESSION   DASFSW010000337 DASFSW010000000
VERSION     DASFSW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.341"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>506
                     /locus_tag="U1099_002306"
     CDS             <1..>506
                     /locus_tag="U1099_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEM4868566.1"
                     /translation="LGNVNPSGKGMNGEVYLSRGIAPTLTRGKGEGTKIAIPVLTPDR
                     LEKRQHGRRFKGNDDPMFTLTSQDRHGVVVAGTLPTSFIQTGRVYDLSGLSPTLTTMQ
                     GGDKVPKILCREEAPHLKIREATKLGYAKAIVGDSVNLAYPESTKRRGRVGKGISNTL
                     TTSDNMGVV"
BASE COUNT          161 a          101 c          121 g          123 t
ORIGIN      
        1 ttaggtaatg ttaatccgtc aggcaaaggg atgaatggag aagtctattt atcacgtggg
       61 atagcaccaa ccttaacaag aggaaaagga gaaggaacta aaattgccat tccagtttta
      121 acccctgata ggctagaaaa acgccaacat gggagacgct ttaaagggaa cgatgatccg
      181 atgtttactt taactagcca agatcgacat ggtgtagttg tcgcaggaac tttaccaact
      241 tcttttatcc aaactggacg tgtctatgac ctttcagggc tttctccaac gttgacaacc
      301 atgcaaggag gggataaagt tcctaagatt ctctgtcgtg aagaagcgcc acatttgaaa
      361 attagggaag caaccaaact aggctatgcc aaggcaatag tgggagattc tgtcaacctt
      421 gcttacccag agtctaccaa acgcagaggt cgagtgggaa aggggatttc aaatacctta
      481 accacttctg acaatatggg agtggt
//
LOCUS       DASFSW010000338          505 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.342, whole genome shotgun sequence.
ACCESSION   DASFSW010000338 DASFSW010000000
VERSION     DASFSW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.342"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>505
                     /locus_tag="U1099_002307"
     CDS             <1..>505
                     /locus_tag="U1099_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEM4868567.1"
                     /translation="EQDPEKVELFLKELNNLSHLTPVYIDETGFETCFHREYGRSLKG
                     QLIKGKVSGRRYQRISLVAGLINGALIAPMTYKDTMTSGFFEAWFKTFLLPTLGKPSV
                     IIMDNAKFHRMSKLKDLCEEQGHRLLPLPPYSPEYNPIEKIWAHIKKHIRRVLTNCDT
                     FLEVLSS"
BASE COUNT          163 a           95 c          103 g          144 t
ORIGIN      
        1 atgaacaaga ccctgaaaaa gtagaactgt tccttaaaga attgaataac ttaagccact
       61 tgactcctgt ttatattgac gagacagggt ttgagacatg ttttcatcga gaatatggtc
      121 gctctttgaa aggtcagttg ataaaaggta aggtctctgg aagaagatac cagcggatat
      181 ctttagtggc aggtctcata aatggtgcgc ttatagcccc gatgacatac aaagatacta
      241 tgacgagtgg ctttttcgaa gcttggttca aaacattctt actacccact ttaggtaaac
      301 catctgttat catcatggac aatgcaaagt ttcataggat gagtaagcta aaagatttat
      361 gcgaggagca gggacataga cttttaccac ttcctcctta ctcaccggaa tataatccca
      421 ttgagaaaat atgggctcac atcaaaaaac acatcagaag agtattgaca aattgcgata
      481 cttttcttga ggtactttcg tcctg
//
LOCUS       DASFSW010000339          503 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.343, whole genome shotgun sequence.
ACCESSION   DASFSW010000339 DASFSW010000000
VERSION     DASFSW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.343"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..333)
                     /locus_tag="U1099_002308"
     CDS             complement(<1..333)
                     /locus_tag="U1099_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HEM4868568.1"
                     /translation="MKKKVFGLLALGSIILTACSFSSAGLSFDEGYQTFHYKGKDYAV
                     SKQEVAENNIQEVEVKFMEWPVVKEEGAVKKTVSLNNLYITTSKEWAIGVQDGYYKVD
                     LEDNIAESD"
     gene            complement(346..486)
                     /locus_tag="U1099_002309"
     CDS             complement(346..486)
                     /locus_tag="U1099_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868569.1"
                     /translation="MSFKFLQVKRMEEKAFTEKEKTELLEQAEFLEELALDLQTMRFM
                     AY"
BASE COUNT          128 a          122 c           74 g          179 t
ORIGIN      
        1 gtctgattca gcaatattat cttctaaatc caccttataa taaccatcct ggacaccgat
       61 ggcccattcc ttgctagtgg ttatatagag attattcaag gatactgtct ttttcactgc
      121 cccctcttct ttgacaacag gccattccat aaacttgact tctacttctt gaatattatt
      181 ctcggcaact tcctgtttgc taacggcata gtcttttccc ttgtaatgga aagtttgata
      241 gccctcatca aagctcaggc ctgctgaaga gaaagaacaa gcggttagga ttatgctgcc
      301 caaagccagc aagccaaata ctttcttttt catagtttcc tccctctagt atgccataaa
      361 tcgcattgtt tgcaaatcaa gtgccagctc ctctaaaaat tctgcctgtt ccaagagttc
      421 agtcttttct ttttctgtga atgccttttc ttccattctc tttacttgta aaaatttaaa
      481 tgacatatga tttccttctt tct
//
LOCUS       DASFSW010000340          499 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.344, whole genome shotgun sequence.
ACCESSION   DASFSW010000340 DASFSW010000000
VERSION     DASFSW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.344"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..227)
                     /locus_tag="U1099_002310"
     CDS             complement(<1..227)
                     /locus_tag="U1099_002310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEM4868570.1"
                     /translation="MAGKYIDDPHLQIGMSQYNKDMVKNSVVNPPNGSPVGTVTLDFD
                     NENGNGEQVYAVVKDPNLPKDQVLEVTVLFRG"
     gene            complement(230..>499)
                     /locus_tag="U1099_002311"
     CDS             complement(230..>499)
                     /locus_tag="U1099_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEM4868571.1"
                     /translation="ATSEEAKKVYEEYLRMEEPAALTKEGKIRTYEVELEELGYNPMG
                     GLMTKIYINNDKKKTMSFNLIDNEDGTYSTAYYILSGELSTFLEE"
BASE COUNT          128 a          117 c           78 g          176 t
ORIGIN      
        1 cctcgaaaga gaaccgtaac ttccaatact tggtctttgg gcaggtttgg atctttcaca
       61 acagcataaa cctgttcacc attgccattt tcattgtcga agtcgagggt gactgtgccg
      121 acaggagagc cattaggggg gttgactaca ctattcttca ccatatcttt gttatactga
      181 ctcatcccaa tttgaagatg aggatcatct atatattttc ctgccatagc tactcctcta
      241 aaaatgttga taattctcca gatagtatgt aataagctgt actatatgtc ccatcttcat
      301 tatcaattag attaaaactc atcgttttct ttttatcatt gttgatataa atctttgtca
      361 ttagaccacc catcggatta tatcccagtt cctccagctc aacctcgtat gttctaattt
      421 tgccttcctt ggtaagagct gctggttctt ccatccttaa atattcctca taaacctttt
      481 tcgcctcttc actagtagc
//
LOCUS       DASFSW010000341          493 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.345, whole genome shotgun sequence.
ACCESSION   DASFSW010000341 DASFSW010000000
VERSION     DASFSW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.345"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            287..>493
                     /locus_tag="U1099_002312"
     CDS             287..>493
                     /locus_tag="U1099_002312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868572.1"
                     /translation="MPDVPSGLPYGRSYALLSYALRLRIKERRHDGYSSCEGVSQKHR
                     HHRHDRHAGDKGDSYGNRQGASQNC"
BASE COUNT          113 a          141 c          130 g          109 t
ORIGIN      
        1 gcgatattgg gataccccct gttactgtca ttgacgctta aagccatgta aagttggcgg
       61 ctctgccctg cgggtgacag cggtgacggc agtgacagtg attttaggat accctgccgc
      121 tgtcatccct acgggagaac accccgcaaa cgcaaaatgc aggcgtggga ttcacccgcc
      181 cttttcgggc gtgtaaaatc acccctgcgg tcagaaaaat ctctccgatt tttccgactg
      241 cgtttacagg gtgtaacaca ctacactttg ccctgcaaag agccgtgtgc cagacgttcc
      301 ctctggactc ccttatggca ggtcttacgc cctgctatcc tacgccttgc ggcttcggat
      361 aaaagaacgg cggcatgacg gttacagctc ttgcgaggga gtatcccaaa aacaccgtca
      421 ccatcgtcat gaccgtcatg caggggataa gggtgacagt tacggcaatc ggcagggggc
      481 atcccaaaac tgc
//
LOCUS       DASFSW010000342          491 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.346, whole genome shotgun sequence.
ACCESSION   DASFSW010000342 DASFSW010000000
VERSION     DASFSW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.346"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>491
                     /locus_tag="U1099_002313"
     CDS             <1..>491
                     /locus_tag="U1099_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEM4868573.1"
                     /translation="YQLIDELCCDKYVYFNCIIEKLVESYGEEYRDIATSYIMSLIDS
                     HFLISNINSELIMNFKFEEFISKVKEIDKQNLYYFKLIAISNLIVEYSELEIGDGIEK
                     LKEIYKLMSSLVETPNFLQIDLYNDGQIQLGNENKTQIVEFAEFLVSNSDHVKRTYLD
                     DYK"
BASE COUNT          183 a           51 c           77 g          180 t
ORIGIN      
        1 ttatcaactt attgatgagc tttgttgtga taaatatgta tattttaact gtattataga
       61 aaaacttgtt gaatcctatg gtgaagagta tcgagatatt gcaacatcat atatcatgag
      121 tttgattgat agtcattttc taatttcaaa tattaattca gaattaataa tgaattttaa
      181 gtttgaagaa ttcatttcta aagtcaaaga aatcgataag caaaatcttt attattttaa
      241 actaatagca attagcaact taattgttga atattctgaa ttggaaattg gggatgggat
      301 tgaaaagctt aaagaaatct acaagttaat gtcatcgtta gttgaaaccc ctaattttct
      361 tcaaattgat ctttacaatg atggtcaaat ccaattaggt aatgaaaata aaactcagat
      421 agtagaattt gctgagtttc tagtaagtaa ttcagatcat gtaaagcgta cttatttaga
      481 tgattataaa g
//
LOCUS       DASFSW010000343          482 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.347, whole genome shotgun sequence.
ACCESSION   DASFSW010000343 DASFSW010000000
VERSION     DASFSW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.347"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>482)
                     /locus_tag="U1099_002314"
     CDS             complement(<1..>482)
                     /locus_tag="U1099_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEM4868574.1"
                     /translation="DSNLGIGSPYGYQHPKNDFWESSPSTVYFSEKEELEYLNNFEKA
                     LETGGNIQLYNEEDFFNQDKDKINLGFELFFYVNKVDGKSVLSLTNTGCSKNLGASSG
                     RFSILSDKLEHYHQTITQIVENNNHVSGYNSCEITFLPENLRHANVMRTTNVREKVLS
                     L"
BASE COUNT          156 a           78 c           69 g          179 t
ORIGIN      
        1 agtgataata ctttttctct gacatttgtt gtgcgcataa cattagcatg acgaagattc
       61 tctggtaaaa atgttatttc acaagagtta taccctgata cgtgattatt gttttcaaca
      121 atttgagtga tagtttgatg ataatgttcc aatttatctg atagaataga aaacctgcct
      181 gagctagctc ctaaattctt agaacatcct gtattagtta agcttagtac gcttttacca
      241 tctactttat taacataaaa gaacaactca aatcctagat tgattttatc tttgtcttga
      301 ttaaaaaaat cttcttcatt ataaagttga atatttccac ctgtttctaa ggctttttca
      361 aaattattga gatattctaa ttcttctttt tcactaaaat atacagtaga aggcgaagat
      421 tcccagaaat catttttggg gtgctgatat ccatatggag aaccaattcc taaattagaa
      481 tc
//
LOCUS       DASFSW010000344          480 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.348, whole genome shotgun sequence.
ACCESSION   DASFSW010000344 DASFSW010000000
VERSION     DASFSW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.348"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>480
                     /locus_tag="U1099_002315"
     CDS             <1..>480
                     /locus_tag="U1099_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucan-binding protein"
                     /protein_id="HEM4868575.1"
                     /translation="AKADNEAIDKRNQAGQKAVDDENKAGQAAVDAYNKDQQKLVTDR
                     EAEITEITKRNKEKEEAAKKENDAIDAYNTKEMERYKRDLAEISKGEEGYISQALAQA
                     LNLNNGEPQARHSADTRNPNRIVAKGDAMLGGYSKILDSIGFFVYDNFKTGETLSFT"
BASE COUNT          173 a           94 c          108 g          105 t
ORIGIN      
        1 ggcaaaggct gataatgaag caattgataa acgcaatcaa gccggacaaa aagcagtgga
       61 tgatgaaaac aaggcaggtc aagctgctgt tgatgcctat aataaagacc aacagaaatt
      121 agtcacagac cgtgaagctg agattacgga aatcacgaaa cgcaataagg aaaaagaaga
      181 agccgccaag aaagaaaatg acgccattga tgcctataac accaaggaaa tggagcgcta
      241 taaacgggat ttagctgaga tttcaaaagg agaggaaggc tatatctctc aagctcttgc
      301 tcaggcactc aacttgaata atggagaacc gcaagcacga cactcagctg ataccagaaa
      361 tcctaatcgc atcgttgcta agggtgacgc tatgcttggt gggtattcta aaatcctaga
      421 ttctattggt ttctttgtct atgataactt taaaacggga gaaacccttt cctttaccta
//
LOCUS       DASFSW010000345          465 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.349, whole genome shotgun sequence.
ACCESSION   DASFSW010000345 DASFSW010000000
VERSION     DASFSW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.349"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..337
                     /locus_tag="U1099_002316"
     CDS             <1..337
                     /locus_tag="U1099_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM4868576.1"
                     /translation="KKVVTNHVDEEGNPIAPQEDGTTPKRQISGYEYVRTVVDEEGNT
                     THIYRKLSNKPTTPEKETPAKPQAGKTASGKAQLPNTGEASSVAGALGTAMLVATLAF
                     ARKRRRNED"
BASE COUNT          158 a           95 c           97 g          115 t
ORIGIN      
        1 taagaaagtt gtgactaacc acgttgatga agaaggtaac cctattgctc cacaagagga
       61 tgggacaaca ccaaaacgtc aaatttcagg ttacgagtat gtgcgtactg tagttgatga
      121 agaaggtaac acgacacata tttatcgcaa actttctaat aaaccaacaa cacctgagaa
      181 ggaaactcct gcaaaacctc aagcaggtaa aaccgcttca ggtaaagctc aattgccaaa
      241 tactggtgag gcttcatctg tggcaggtgc gcttggtaca gcaatgcttg tcgcaacact
      301 tgcgtttgca agaaaacgtc gtcgtaacga agattagtca aaattcttta tacagacttt
      361 attcccccat atagaaagta taagaattgt acgtaacatg caggattgcc tttccgaaaa
      421 aatgaggctg tgcaaaaagt ccagagttac atcttagagt tcgct
//
LOCUS       DASFSW010000346          448 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.350, whole genome shotgun sequence.
ACCESSION   DASFSW010000346 DASFSW010000000
VERSION     DASFSW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.350"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..333
                     /locus_tag="U1099_002317"
     CDS             <1..333
                     /locus_tag="U1099_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM4868577.1"
                     /translation="TALEYSLKYETTFRTVLSDGDLVLSNNVAERAMKTLVMGRKNWL
                     FSQSFEGAKSTAIILSLLETAKRHELDTEKYMTYLLDNLPNEETLAKKEVLEAYLPWE
                     KKIKRACK"
BASE COUNT          145 a           83 c          108 g          112 t
ORIGIN      
        1 actgctttgg agtatagcct caaatacgaa accaccttcc gaaccgttct ctcggatggt
       61 gacttggtcc tgtctaacaa cgtggctgaa agggctatga agaccttggt gatgggcaga
      121 aaaaattggt tgttctccca aagttttgag ggagccaagt cgacagctat cattttgagt
      181 cttttggaaa cggccaagag gcacgaactt gatacagaga aatatatgac ctatcttcta
      241 gacaatctac ctaacgagga aacactcgct aaaaaagaag ttctagaggc ttatttgcca
      301 tgggagaaga aaattaagag agcatgtaaa tagaaaaagg ttccagagtc aacaggactt
      361 tggagccttt tcaatatacc ttattattgg gcggttacaa agtagcctag gaaacgaagc
      421 cgaagataga actggagttg gagttcat
//
LOCUS       DASFSW010000347          443 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.351, whole genome shotgun sequence.
ACCESSION   DASFSW010000347 DASFSW010000000
VERSION     DASFSW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.351"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            58..339
                     /locus_tag="U1099_002318"
     CDS             58..339
                     /locus_tag="U1099_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEM4868578.1"
                     /translation="MARKESMAERNFKMKKDIHPEYRTVVFMDTTTGYKFLSGSTKKS
                     NETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
BASE COUNT          159 a           83 c           84 g          117 t
ORIGIN      
        1 aggtaaaaag acttgccata agaacaaact tttggtaaac ttaaatggtt ataatctatg
       61 gctcggaaag agtccatggc agaaaggaat tttaaaatga aaaaagatat ccatccagaa
      121 tatcgcactg ttgtcttcat ggacacaact actggctaca agttccttag cggttcaact
      181 aagaaatcta acgaaacagt tgaattcgaa ggtgaaactt acccattgat ccgtgtggaa
      241 atttcatcag actcacaccc attctatact ggacgtcaaa agttcactca agcagatgga
      301 cgtgtggatc gcttcaacaa aaaatacggt ctcaaataaa aaaacagtcc ttaacggact
      361 gttttttcac gagcttgaaa ataaaagagc gagttatggt ccagtggacc attttttctt
      421 tgcctataga aataaaagag gtc
//
LOCUS       DASFSW010000348          437 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.352, whole genome shotgun sequence.
ACCESSION   DASFSW010000348 DASFSW010000000
VERSION     DASFSW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.352"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>437
                     /locus_tag="U1099_002319"
     CDS             <1..>437
                     /locus_tag="U1099_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM4868579.1"
                     /translation="ITGIKKKINENKIADKGKTFEELVEEAKIAVGKGGPLIIDVVNS
                     IPELLAFYDEIKTCSFEEVSNKSKVNKESLLAYKFNGLSSRYEAGTDRDRILKRLDLI
                     YELLELYKTGKHNEFLRITKFNITSSEDKISLSKVMTKISVDD"
BASE COUNT          174 a           44 c           88 g          131 t
ORIGIN      
        1 taattacagg tataaagaaa aaaatcaatg aaaataagat agctgataaa ggaaaaacgt
       61 ttgaagaact ggtagaagag gctaaaatag ctgtcggaaa gggaggtcca ttaataattg
      121 atgtggttaa ttccatccca gaacttttgg ctttttacga tgaaattaag acgtgctcgt
      181 ttgaagaagt tagtaataag agtaaagtta ataaagaatc gctgttagct tataaattta
      241 atggattaag tagtagatat gaagcgggca cagataggga taggattctt aaaaggttag
      301 atttaatcta tgagctatta gagctgtata aaacaggtaa gcataatgaa ttcttacgta
      361 ttactaaatt taatataact tcatcagaag ataaaattag tctatctaaa gttatgacaa
      421 aaatctctgt tgatgat
//
LOCUS       DASFSW010000349          417 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.353, whole genome shotgun sequence.
ACCESSION   DASFSW010000349 DASFSW010000000
VERSION     DASFSW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.353"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>417
                     /locus_tag="U1099_002320"
     CDS             <1..>417
                     /locus_tag="U1099_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecF"
                     /protein_id="HEM4868580.1"
                     /translation="RYSVNELVRTADGEKITEFISDLMLKNKNLPRPNYGLRTQTLES
                     FEAKLQELDKYRRVRKDSILKIIDDFNQTQVGKLRICYEQIDRWEVPDFESTLFKSNS
                     TLNFSFENYRISKRDVADILYLVYQELGIKGFVNVIL"
BASE COUNT          157 a           52 c           76 g          132 t
ORIGIN      
        1 agatattctg ttaatgagtt ggttagaaca gcggatggag aaaaaattac agaatttatt
       61 tccgacttaa tgctaaaaaa taaaaattta ccgagaccaa actatggttt aagaactcag
      121 actctagaat cattcgaagc taaactacag gaacttgata aatataggag agttcgtaag
      181 gattcaatac tcaaaataat tgatgatttc aatcaaactc aagttggtaa gttaagaatc
      241 tgttatgagc aaattgatag atgggaagta ccagattttg aaagcactct tttcaaaagt
      301 aattcaactc tcaatttctc atttgaaaat tatagaatta gtaagcggga tgttgcagat
      361 attctatacc ttgtttatca ggagttagga ataaaaggat ttgttaacgt tattttg
//
LOCUS       DASFSW010000350          416 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.354, whole genome shotgun sequence.
ACCESSION   DASFSW010000350 DASFSW010000000
VERSION     DASFSW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.354"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>416
                     /locus_tag="U1099_002321"
     CDS             <1..>416
                     /locus_tag="U1099_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868581.1"
                     /translation="GEARKLAIDMRLIDPVYTLSDNQKILQVVDNVERIYLEGAGEKA
                     TQMIFSDIGTPKSKEEGFDVYNELKALLVERGIPKEEIAFVHDANTDEKKNSLSRNVN
                     SGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPS"
BASE COUNT          134 a           75 c          100 g          107 t
ORIGIN      
        1 ggggaagcta gaaaactagc tattgatatg cggttgattg accctgttta taccttatca
       61 gataatcaga aaatccttca agtggttgat aatgtcgagc gaatctatct tgaaggagca
      121 ggagaaaagg ctactcagat gattttctct gatattggaa cacctaaaag taaggaagaa
      181 ggttttgatg tctacaatga actgaaagct ttgctggttg aacgagggat tccaaaagaa
      241 gaaattgcct ttgtccatga tgccaatact gatgagaaga aaaactcact ctcacgaaat
      301 gtcaatagtg gggaagtacg gattctcatg gcttctacgg aaaaaggggg aacaggatta
      361 aacgtccagt ctcgcatgaa agctgtccac catttagacg ttccctggag gccctc
//
LOCUS       DASFSW010000351          403 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.355, whole genome shotgun sequence.
ACCESSION   DASFSW010000351 DASFSW010000000
VERSION     DASFSW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.355"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>403)
                     /locus_tag="U1099_002322"
     CDS             complement(<1..>403)
                     /locus_tag="U1099_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868582.1"
                     /translation="FNGFKTLKSYSVTKEIISLSDKKLDKLEDSTFNLKRSEVLSKLV
                     AVLIAGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKVLGPIQSIAYSLNKINTT
                     KDLRKPFLKYLSGEKNFINAEYDNYGLYTSTI"
BASE COUNT          150 a           66 c           48 g          139 t
ORIGIN      
        1 tattgttgaa gtatacagtc cgtaattatc atattcagcg tttataaaat tcttctctcc
       61 acttaagtat tttaaaaacg gtttccttaa atctttggtt gtatttatct tatttaatga
      121 ataggcaatt gattgtatcg gccctaaaac tttatcgttt gctaagaaaa tacctatcag
      181 ttcactaaaa gaaaggcttt tatgataaat tacaaaataa caacctacaa ccaagggaac
      241 tagaaagcaa aaacctgcaa ttagtactgc aaccaatttt gaaagaacct ctgaacgttt
      301 caaattaaaa gtagaatctt ctagtttatc caacttttta tccgacaaac taattatttc
      361 tttagtaaca gaataagatt ttaatgtctt aaaaccatta aaa
//
LOCUS       DASFSW010000352          401 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.356, whole genome shotgun sequence.
ACCESSION   DASFSW010000352 DASFSW010000000
VERSION     DASFSW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.356"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>401
                     /locus_tag="U1099_002323"
     CDS             <1..>401
                     /locus_tag="U1099_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEM4868583.1"
                     /translation="EARKLAIDMRLIDPAYTLSDNQKILQVVDNVERIYRDGAEDKAT
                     QMIFSDIGTPKSKEEGFDVYNELKDLLVDRGIPKAEIAFVHDANTDEKKNSLSRKVNS
                     GEVRILMASTEKGGTGLNVQSRMKAVHHLDVP"
BASE COUNT          137 a           77 c           87 g          100 t
ORIGIN      
        1 gaagccagaa aactagccat tgatatgcga ttgattgacc ctgcttatac cttatcagac
       61 aatcagaaaa tccttcaagt ggtcgataat gtcgaacgaa tctaccgtga tggagctgaa
      121 gacaaagcca ctcagatgat tttctctgat attggaacac ctaaaagtaa ggaagaaggt
      181 tttgatgttt ataatgaact caaagaccta ctggttgatc gagggattcc aaaagcagaa
      241 attgcctttg tccatgatgc caatactgat gaaaagaaaa actcactgtc tagaaaggtc
      301 aatagcggag aagtacggat tctcatggct tcgactgaaa aaggtggaac gggtctaaac
      361 gtacaatcac gcatgaaagc tgttcaccac ttagatgttc c
//
LOCUS       DASFSW010000353          401 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.357, whole genome shotgun sequence.
ACCESSION   DASFSW010000353 DASFSW010000000
VERSION     DASFSW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.357"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>401
                     /locus_tag="U1099_002324"
     CDS             <1..>401
                     /locus_tag="U1099_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868584.1"
                     /translation="EARKLAIDMRLIDPAYTLSDNQKILQVVDNVERIYREGNLEKAT
                     QMIFSDIGTPKNKEEGFDVYNELKDLLMDRGIPKEAIAFVHDANTDDKKNSLSRKVNS
                     GEVRILMASTEKGGTGLNVQSRMKAVHHLDVP"
BASE COUNT          131 a           75 c           91 g          104 t
ORIGIN      
        1 gaggctagaa aactagctat tgacatgcgt cttattgatc cagcctatac cttatctgac
       61 aatcagaaga ttctgcaagt ggtggataac gtggaacgga tttaccgtga gggcaatctc
      121 gagaaagcta cccagatgat tttctcagat attggaactc ctaagaataa ggaagagggc
      181 tttgacgttt acaatgaatt gaaagaccta ttaatggata gagggattcc caaagaagca
      241 attgcctttg tccatgacgc caataccgat gataagaaaa attctctgtc acgcaaagtc
      301 aatagtgggg aagtacggat tctcatggct tctactgaaa aaggtggaac aggattaaac
      361 gtccaatcac gtatgaaagc tgttcatcat ttagacgttc c
//
LOCUS       DASFSW010000354          392 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.358, whole genome shotgun sequence.
ACCESSION   DASFSW010000354 DASFSW010000000
VERSION     DASFSW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.358"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(7..>392)
                     /locus_tag="U1099_002325"
     CDS             complement(7..>392)
                     /locus_tag="U1099_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4868585.1"
                     /translation="QGCLLEVVREQYTDFYRDKERWRYLQKLDTKNRLLSLDGFTDSE
                     GNPLDFITDEAVDIAETVVNAVMVDRLKAALPLLSDSEQELIQAIFFDGLSEREVGAR
                     LGITQSVVNKRKARILIKLRKIIEN"
BASE COUNT           90 a          104 c           77 g          121 t
ORIGIN      
        1 taaattttaa ttttctatta tctttcttag ttttattagg attctggctt tgcgtttgtt
       61 tacaacgctc tgggttatgc ccaacctcgc cccgacttca cgctcggaaa gtccgtcaaa
      121 aaagattgcc tgtatcagct cctgttcact atccgacagc aaaggcaggg cggctttcag
      181 cctgtccacc atgaccgcat tgacaacggt ttctgcaatg tccaccgctt catcagtgat
      241 aaagtccaga ggattcccct cgctgtccgt aaatccgtcg agagatagca gtctattctt
      301 tgtatctaat ttttgcagat aacgccaccg ttccttgtca cggtagaagt ctgtgtattg
      361 ctccctcacg acttcaagca gacagccttg aa
//
LOCUS       DASFSW010000355          378 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.359, whole genome shotgun sequence.
ACCESSION   DASFSW010000355 DASFSW010000000
VERSION     DASFSW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.359"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
BASE COUNT          155 a           49 c           60 g          114 t
ORIGIN      
        1 agagctaaac aaaagacagt aaaattaacg ccgaaaacag tagtaaaaaa tcatccccaa
       61 aaatcttcgg tagaaaaaag gaggcagaga tatttggata aagttttctc aaaaaatcca
      121 aatattcagg ttttagaata tattgattca aaaactcctg tccattataa atgttgtcaa
      181 tgccaatttg aatggaaggc aagaagtgat aaaatattca ttcgtcccta ttgtaagaat
      241 tgcaaatcaa tctccattga taaaaattag attttctagc aattcaatta ttttaattgt
      301 ttagataatt ttatgatgaa aaaataaaat cagtcccaag gctgatgaga tagttgatag
      361 gatgagttat actatagt
//
LOCUS       DASFSW010000356          378 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.360, whole genome shotgun sequence.
ACCESSION   DASFSW010000356 DASFSW010000000
VERSION     DASFSW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.360"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..337
                     /gene="mscL"
                     /locus_tag="U1099_002326"
     CDS             <1..337
                     /gene="mscL"
                     /locus_tag="U1099_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEM4868586.1"
                     /translation="RGNIVDLAVAVVIGGAFGAIIKSFVNDIITPLILNPALKAANVQ
                     NIADLAWNGVKYGSFLSAVINFLIIGTSMFFVVKAAEKAMPKKVEEKAPAGPTQEELL
                     AEIRDLLKK"
BASE COUNT          114 a           65 c           88 g          111 t
ORIGIN      
        1 ccgtgggaac attgttgacc ttgctgtagc agtggtaatt ggtggagcgt tcggcgcaat
       61 tatcaaatca tttgtaaatg atatcatcac tccattgatt ttgaacccag ctttgaaagc
      121 ggcaaatgta caaaatattg ctgaccttgc atggaatggt gtgaagtacg gtagtttctt
      181 gagcgctgtt attaacttcc taatcattgg cacttcaatg ttctttgttg ttaaagcagc
      241 tgagaaagca atgcctaaga aagttgaaga gaaagctcct gctggtccaa ctcaagaaga
      301 gttgcttgca gaaatccgcg atttgttgaa aaaataatca ataatatatt gggagtggga
      361 aagaactcga ccagtcta
//
LOCUS       DASFSW010000357          370 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.361, whole genome shotgun sequence.
ACCESSION   DASFSW010000357 DASFSW010000000
VERSION     DASFSW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.361"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            17..>370
                     /locus_tag="U1099_002327"
     CDS             17..>370
                     /locus_tag="U1099_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM4868587.1"
                     /translation="MYKFWQKTIQVLSILTLIGTAIFLFWLYKIGILNDQNVLSDLIK
                     SQGALGSLSFLAIQIIQVVFPIIPGGVTTVVGFLVFHFWWGFFLNYLGISIGSIILFW
                     LARRYGKKFCLLFMSE"
BASE COUNT           90 a           62 c           73 g          145 t
ORIGIN      
        1 atggtaaaat gaaagtgtgt ataaattttg gcaaaaaacc attcaagtat taagtatttt
       61 gacccttatc gggactgcta tttttctgtt ttggctctat aagattggca ttttgaacga
      121 ccaaaatgtc ttaagcgatt taatcaaaag ccaaggtgct ttaggtagcc tctccttttt
      181 agctattcag attattcaag ttgtatttcc gattattcca ggcggtgtta cgactgttgt
      241 gggtttcctc gtatttcatt tttggtgggg attctttctc aattatcttg gaatttcaat
      301 cggtagtatc atccttttct ggttggctcg tcgctatgga aagaaatttt gtctcctctt
      361 catgtctgaa
//
LOCUS       DASFSW010000358          368 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.362, whole genome shotgun sequence.
ACCESSION   DASFSW010000358 DASFSW010000000
VERSION     DASFSW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.362"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>368)
                     /locus_tag="U1099_002328"
     CDS             complement(<1..>368)
                     /locus_tag="U1099_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HEM4868588.1"
                     /translation="CAIFLYYFFGFLITHSDSYAHRVNGLINFFEYYRNDWFHLMFGA
                     ADLAYGDLTLDYAIRVRRVLGWNGTLEMPLLSIMLKNGFIGLVGYGIVLYKLYRNVRI
                     LKTDNIKTIGKSVFIIVVLS"
BASE COUNT          147 a           74 c           41 g          106 t
ORIGIN      
        1 cagataggac tacaatgata aatacagact ttcctattgt ttttatatta tctgttttta
       61 atattcttac attacgataa agtttatata aaacaatccc ataccctacc agaccgataa
      121 aaccattttt taacatgata ctcagtaagg gcatttcaag cgttccattc caacctaaaa
      181 cgcgtctaac ccttatagca tagtctaaag ttaaatcccc atatgccaaa tccgctgcac
      241 caaacattag atggaaccaa tcatttctat aatactcaaa aaaattaata agaccattaa
      301 cgcgatgagc gtaagaatca ctatgtgtta ttaaaaaacc aaagaaatag tataaaaaaa
      361 tagcacaa
//
LOCUS       DASFSW010000359          365 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.363, whole genome shotgun sequence.
ACCESSION   DASFSW010000359 DASFSW010000000
VERSION     DASFSW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.363"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
BASE COUNT          141 a           52 c           48 g          124 t
ORIGIN      
        1 gaaatttaac cacccgactg tcatgccatt tagtaacttc tcaattggat tttgctttct
       61 aatttgatat gaaaatttat taaactgata ctttatcgat tttattgtat ctttggattt
      121 atggatactt atcacaccag agtcatagat agccatacaa attagaaaat ataatcctac
      181 taaaatcgaa agaatcgttg ctatatacaa tgaattaata ttaaatattt gacgtagtac
      241 tatatcaatt gttccaccca atattacaat gataaatgga aagaaataaa aattggttag
      301 gagaaaagtt aaaccaaata taacaaaacc tataatccca ggtatgatta aggattttgc
      361 agttt
//
LOCUS       DASFSW010000360          363 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.364, whole genome shotgun sequence.
ACCESSION   DASFSW010000360 DASFSW010000000
VERSION     DASFSW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.364"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..230)
                     /locus_tag="U1099_002329"
     CDS             complement(<1..230)
                     /locus_tag="U1099_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM4868589.1"
                     /translation="MKFLDLFAGIGGFRLGLTRQGHECIGFCEVDKFARKSYKAIYET
                     KGEIEFHDIRQVTDQDFRQLRGQVDIICGGFP"
BASE COUNT          107 a           97 c           52 g          107 t
ORIGIN      
        1 caagggaatc caccacagat gatgtccact tgccctctaa gttgtctaaa gtcctgatct
       61 gtgacttgtc taatatcatg aaattctatt tctcctttgg tctcatagat ggccttgtaa
      121 gatttcctcg caaacttgtc aacctcacaa aagccaatac actcatggcc ctgacgggtt
      181 aaccccagtc gaaagcctcc aattcctgca aacaaatcta aaaacttcat gtctctcatt
      241 cctttctgac aaaactaaaa ggaccttact tcttgcgtaa cgtcctttct taacaatgat
      301 cttccatttc tggggcagtg ataagttcaa aaatgaacca aatcattcct agtccccaaa
      361 tac
//
LOCUS       DASFSW010000361          358 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.365, whole genome shotgun sequence.
ACCESSION   DASFSW010000361 DASFSW010000000
VERSION     DASFSW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.365"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            1..358
                     /locus_tag="U1099_002330"
                     /pseudo
     CDS             1..358
                     /locus_tag="U1099_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922520.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          130 a           45 c           59 g          124 t
ORIGIN      
        1 acgaatcttt gttttataga gtttgtcttc ggtataacta aaatttttga tatcagagaa
       61 aaaaatggta tttttaagat tctatctaag ttgagacata gtaccaaaga tagctctctt
      121 ttatcaattt tgaatactat tcaagatggt attgataaag aacaaaaaaa tattgatgag
      181 attaaattgc ttcgcgacaa actcttggct cacttagata aagagatggt cttttcaaca
      241 gaaagattag acattggcat tctatactat tattttgagg cgatagaaat taagtccata
      301 tatacagctt gtatcgagtt atataatact ttatatggag ataatcagca acaagtcg
//
LOCUS       DASFSW010000362          349 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.366, whole genome shotgun sequence.
ACCESSION   DASFSW010000362 DASFSW010000000
VERSION     DASFSW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.366"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            66..>349
                     /locus_tag="U1099_002331"
     CDS             66..>349
                     /locus_tag="U1099_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM4868590.1"
                     /translation="MRVVFGIDVSKVSSEVAILVNGEKVHNYTMSNDAIGFSRLLGDL
                     KTVHKPEIIFEATGVYSRRLQSFLDEHGYAYTRLNPLEAKKQLDSLRVRKT"
BASE COUNT          113 a           72 c           75 g           89 t
ORIGIN      
        1 agacgtcgag tattgaaaat gacatcacta aaacaaggaa tcattcaaga caaagaggta
       61 atatcatgcg agtagtattt gggattgacg tgagtaaagt aagttcagaa gtagccattc
      121 tagtcaacgg cgagaaggtt cataactaca ccatgtccaa tgatgccatt ggcttttctc
      181 ggctacttgg cgatttgaaa accgtccaca agccagaaat catctttgaa gcaacaggcg
      241 tctattctcg tcgtcttcaa tcttttctgg atgaacatgg ctacgcttat acacgactca
      301 atcccttaga agccaagaag caactggata gcttgcgtgt gagaaaaac
//
LOCUS       DASFSW010000363          346 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.367, whole genome shotgun sequence.
ACCESSION   DASFSW010000363 DASFSW010000000
VERSION     DASFSW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.367"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..254)
                     /locus_tag="U1099_002332"
     CDS             complement(<1..254)
                     /locus_tag="U1099_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM4868591.1"
                     /translation="MKFLDLFSGIGGFRLGLTRQGHECIGFCEIDKFARKSYKAIYET
                     EGEIEFHDIRQVTDQDFRQLRGQVDIICGGFPCQAFSLAG"
BASE COUNT           97 a           90 c           57 g          102 t
ORIGIN      
        1 ctgcctgcga gtgaaaatgc ctggcaaggg aatcccccac agatgatgtc cacttgccct
       61 ctaagttgcc taaagtcttg gtctgtgact tgtctaatat catgaaattc tatttctcct
      121 tcggtctcat agatggcctt gtaagatttc ctcgcaaact tatcaatctc acaaaagcca
      181 atacactcat ggccctgacg ggttaaaccc agtcgaaagc ctccaattcc tgaaaacaaa
      241 tctaaaaatt tcatgtctct cattcctttc tgacaaaact aaaaaggacc ttacttcttg
      301 cgtaacgtcc ttgcttaaca atgatcttcc atttctgggg cagtaa
//
LOCUS       DASFSW010000364          345 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.368, whole genome shotgun sequence.
ACCESSION   DASFSW010000364 DASFSW010000000
VERSION     DASFSW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.368"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>345
                     /locus_tag="U1099_002333"
     CDS             <1..>345
                     /locus_tag="U1099_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868592.1"
                     /translation="LTDEIQSGFTYVEKDIMIPPLFRKIPFQPILAYPNGHLSTTMIT
                     LSVEKNTGLNDSVIQNATLIPLEEIIAKLESSTNEDTQFIINSNVGSKLLFEAFSSSL
                     DSEQLFDILQNED"
BASE COUNT          115 a           56 c           50 g          124 t
ORIGIN      
        1 ttgacagatg aaattcaatc aggttttacc tatgttgaaa aagatattat gatacctcca
       61 ttgtttagaa agattccttt tcaacctatt ttagcttatc ctaatgggca cctctcaaca
      121 actatgatta ctctttctgt tgaaaaaaat acaggattaa atgatagtgt cattcaaaat
      181 gcaactttga tcccattaga ggaaattatt gctaagcttg aaagttctac taatgaggat
      241 actcaattta ttataaattc aaatgttggc agtaaacttc tatttgaagc attttcttct
      301 tcacttgact cagagcaatt gtttgatatt ctccagaatg aagat
//
LOCUS       DASFSW010000365          343 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.369, whole genome shotgun sequence.
ACCESSION   DASFSW010000365 DASFSW010000000
VERSION     DASFSW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.369"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>343
                     /locus_tag="U1099_002334"
     CDS             <1..>343
                     /locus_tag="U1099_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868593.1"
                     /translation="GQVSNIPHINLEELKNNSNLLLKEHSKVKKEKQEYDKLISEQIS
                     QIVTLKKPLLELILPHKNFHPFELGHREIRYYAAPIPIENTVYIDFISNTYGKSKSSF
                     FSDFAYVVSVRV"
BASE COUNT          135 a           51 c           51 g          106 t
ORIGIN      
        1 ggtcaagtta gtaatatacc tcatattaat ttggaggaat taaaaaataa tagtaatttg
       61 ttgctaaaag aacactccaa agtaaaaaaa gaaaaacaag aatatgataa attgataagc
      121 gagcagattt ctcaaattgt tacgttaaaa aaaccattgc ttgaacttat tttaccccac
      181 aaaaactttc atccattcga actaggtcat cgagaaataa gatactatgc ggcgccaatt
      241 cctatcgaaa acacggtata tatagatttt atttctaata catatggtaa aagtaaaagc
      301 tcattctttt ctgattttgc ttatgtcgta tcggtaagag taa
//
LOCUS       DASFSW010000366          341 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.370, whole genome shotgun sequence.
ACCESSION   DASFSW010000366 DASFSW010000000
VERSION     DASFSW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.370"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            92..341
                     /locus_tag="U1099_002335"
                     /pseudo
     CDS             92..341
                     /locus_tag="U1099_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521636.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT           86 a           83 c           89 g           83 t
ORIGIN      
        1 ctcacttttt tcgtgggagt aaatctattt ttaaggaggt caacgcctat gtgcaacaaa
       61 aacaagataa ctgcccgaaa ggaggaaacc catgcagaag ttacagacag tcaacgccga
      121 aacgctcctt tatgagccgc ttgagaagcc gtcctttgtg gtggacagcc ttatccccac
      181 aggcttgtcg ctgttctgcg gctcacagaa gataggcaag agctggctca tgctgaagct
      241 gtgcttatgc gtgtcgcagg gaatcccctt atgtatggga catgacgaca atggagggcg
      301 atgtgcttta cctctgcctt gaggacacgt tctgccgcat a
//
LOCUS       DASFSW010000367          340 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.371, whole genome shotgun sequence.
ACCESSION   DASFSW010000367 DASFSW010000000
VERSION     DASFSW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.371"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>340)
                     /locus_tag="U1099_002336"
     CDS             complement(<1..>340)
                     /locus_tag="U1099_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868594.1"
                     /translation="YIKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIV
                     YVGAPILIVLANIISIAFLSSHQEKIDIQQFEILGTSNYQMVKIKFGEAIFLTFVTSL
                     IAFLLNIKIIA"
BASE COUNT          134 a           50 c           45 g          111 t
ORIGIN      
        1 agctataatt ttgatgttaa gtaaaaatgc aattaatgaa gtaacaaatg tcaaaaatat
       61 agcctcacca aatttaatct taaccatctg gtaattagaa gtccctaaaa tttcaaattg
      121 ctgaatatca atcttttctt gatgcgatga caaaaatgca attgaaataa tatttgcaag
      181 tactatcaaa attggtgcac ctacatagac aataaatgcc acttttagct ctaaatcact
      241 gtcatcttga aattgagata gtatattctg agaaatcatt tgaaaactag aaattagtaa
      301 tatagctcct gtaattgcag cactgataga ttttatataa
//
LOCUS       DASFSW010000368          321 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.372, whole genome shotgun sequence.
ACCESSION   DASFSW010000368 DASFSW010000000
VERSION     DASFSW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.372"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
BASE COUNT          130 a           50 c           50 g           91 t
ORIGIN      
        1 aattaaaata agatttaaag taagagagcc tttcaacttt agcaactcac tttttagaat
       61 tttgttcatt agttatcctc cacttcaaca aactaaggaa actcacaaaa actaagattg
      121 aaagagatac tgatattaaa attgtcgata tttcaactcc acttaatgct gcctctaaat
      181 cacctgaggg ttttaaatgt aaaagagcat aagcaatttt atagtgataa gtataaggaa
      241 atatgaacca aaccgatgtc tgagctatta gaggagcaac taataagcaa ataagagaat
      301 ttgtagccaa caacaagtag g
//
LOCUS       DASFSW010000369          317 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.373, whole genome shotgun sequence.
ACCESSION   DASFSW010000369 DASFSW010000000
VERSION     DASFSW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.373"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            27..>317
                     /locus_tag="U1099_002337"
     CDS             27..>317
                     /locus_tag="U1099_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM4868595.1"
                     /translation="MAYSLDFRKKVLAYCEKTGSITEASAIFQVSRNTIYQWLKLKEK
                     TGELHHQVKGTKPRKVDRDKLKNYLETHPDAYLTEIASEFDCHPTAIHYALKA"
BASE COUNT          115 a           59 c           51 g           92 t
ORIGIN      
        1 cagtacattt atgatataat gtatttatgg catattcatt agattttcgt aaaaaagttc
       61 tcgcatactg tgagaaaacc ggcagtatta ctgaagcatc agctattttc caagtttcac
      121 gtaacactat ctatcaatgg ctaaaattaa aagagaaaac cggcgagctt catcaccaag
      181 ttaaaggaac caagccaaga aaagtggata gagataaatt aaagaattat cttgaaactc
      241 atccagatgc ttatttgact gaaatagctt ctgaatttga ctgtcatcca acagctattc
      301 attacgccct caaagct
//
LOCUS       DASFSW010000370          315 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.374, whole genome shotgun sequence.
ACCESSION   DASFSW010000370 DASFSW010000000
VERSION     DASFSW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.374"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(1..315)
                     /locus_tag="U1099_002338"
                     /pseudo
     CDS             complement(1..315)
                     /locus_tag="U1099_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
BASE COUNT          117 a           54 c           38 g          106 t
ORIGIN      
        1 tgttatcaac atacaatttt gtcggaagat tgttttcaag aataatctga tgaatatcct
       61 tcgacaacaa agcctccaac gttcaggagc aacaataatg tccttaaata ctattctagg
      121 aatatgagaa aatttttgat aaaccatctc ccaagggaat attccgaaat gatcttcttg
      181 agctatctca cataaaaacc gtaattcatt gctaaacatc atttggttat acatatttgt
      241 actataaaat tttaagagtt ccttagtttt aaaatttcta gcataaaaac tattcttaga
      301 atcaatacca atata
//
LOCUS       DASFSW010000371          311 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.375, whole genome shotgun sequence.
ACCESSION   DASFSW010000371 DASFSW010000000
VERSION     DASFSW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.375"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(134..301)
                     /locus_tag="U1099_002339"
     CDS             complement(134..301)
                     /locus_tag="U1099_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HEM4868596.1"
                     /translation="MNNEDFNLDLVTISKENNSGASPRVTSKSLCTPGCKTGILMTCA
                     IKTATCGCHFG"
BASE COUNT          111 a           55 c           46 g           99 t
ORIGIN      
        1 agacatattc gtctaaagag taatatccta aacaaatatt ctaatattta gagggacctt
       61 actcgaaaag atatagagtc cctttctttc ttagtcaaga tttgcaaaaa gatagttact
      121 aaacaaataa attttatcca aaatggcaac cacaagttgc agttttgatt gcacaggtca
      181 tcaaaatacc cgtcttacat ccaggagtac ataatgattt actagttacc cgaggtgaag
      241 ctcctgagtt gttttccttt gagattgtga caagatccaa attaaaatct tcattgttca
      301 taataaaatt c
//
LOCUS       DASFSW010000372          299 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.376, whole genome shotgun sequence.
ACCESSION   DASFSW010000372 DASFSW010000000
VERSION     DASFSW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.376"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            <1..>299
                     /locus_tag="U1099_002340"
     CDS             <1..>299
                     /locus_tag="U1099_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM4868597.1"
                     /translation="IFDSHLVEKESTTHISDIVVPVFAKDEIKEPRYIKEILPEVIPT
                     NVRQKIPFDEWLYFKLYMSADRQEEFLSDIMPQILKLVNLDDGMSFYIRYTDPKF"
BASE COUNT          104 a           41 c           55 g           99 t
ORIGIN      
        1 tatttttgat tcacaccttg tagagaagga atctacaact catatttctg atatagttgt
       61 tccagtattt gctaaggatg aaattaagga acctaggtac ataaaagaaa tattaccaga
      121 ggttattccg acaaatgtta gacagaaaat accatttgat gaatggctct actttaaatt
      181 gtatatgtcg gcagataggc aagaggaatt tctttcggat ataatgcctc aaattttgaa
      241 gttagtaaat ttagatgatg ggatgagctt ctatatcaga tatactgatc caaaatttc
//
LOCUS       DASFSW010000373          297 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.377, whole genome shotgun sequence.
ACCESSION   DASFSW010000373 DASFSW010000000
VERSION     DASFSW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.377"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            70..>297
                     /locus_tag="U1099_002341"
     CDS             70..>297
                     /locus_tag="U1099_002341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4868598.1"
                     /translation="MEYYLIVRGRKIFVDKKVYSGYWSLVNHQNYLRRREILFSVLPF
                     SSFEKENFRLEDVLPDLTVDIEKIVETKIMLE"
BASE COUNT          105 a           31 c           60 g          101 t
ORIGIN      
        1 ttcaagtatg agtaggacac ggagtcctcc aattgtactt aataagtttt tagcaattgg
       61 aggaatacta tggaatatta tttaattgta cgaggacgga agatttttgt agataaaaaa
      121 gtatatagtg gttactggag tctggtaaat catcaaaact atttacgaag acgtgaaata
      181 ttattttccg tacttccgtt ttcatcgttt gaaaaagaaa actttagatt agaagatgtt
      241 ttacctgatt tgactgtaga tattgagaaa attgtagaaa caaaaattat gttggag
//
LOCUS       DASFSW010000374          296 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.378, whole genome shotgun sequence.
ACCESSION   DASFSW010000374 DASFSW010000000
VERSION     DASFSW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.378"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..>296)
                     /locus_tag="U1099_002342"
     CDS             complement(<1..>296)
                     /locus_tag="U1099_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4868599.1"
                     /translation="QGCLLEVVREQYADFYRDRERWRYLQKLDARNSLLSLDGFTDSE
                     GNPLDFIADEAADIAETVVNAVMVDRLKAALPLLSDSEQELIQAIFFDGLSERE"
BASE COUNT           74 a           92 c           61 g           69 t
ORIGIN      
        1 ttcacgctcg gaaagtccgt caaaaaagat tgcctgtatc aactcctgct cactatccga
       61 caacaaaggc agggcggctt tcagcctgtc caccataaca gcattgacaa cggtttccgc
      121 aatgtccgcc gcttcatcag cgataaagtc cagaggattc ccctcgctgt ccgtaaatcc
      181 gtccagagat agcaggctgt tcctcgcatc taatttttgc aggtaacgcc accgctccct
      241 gtcacggtag aagtccgcat attgctccct tacgacttca agcagacaac cttgga
//
LOCUS       DASFSW010000375          292 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.379, whole genome shotgun sequence.
ACCESSION   DASFSW010000375 DASFSW010000000
VERSION     DASFSW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.379"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            complement(<1..105)
                     /locus_tag="U1099_002343"
     CDS             complement(<1..105)
                     /locus_tag="U1099_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM4868600.1"
                     /translation="MVKYAPRKVYIRESGGYVELSYTEFCRCRESDQTY"
BASE COUNT           94 a           66 c           55 g           77 t
ORIGIN      
        1 ataggtctgg tcggattccc tgcaacggca gaactccgtg taggataatt ccacatagcc
       61 gccactttct ctgatatata cctttcttgg tgcatatttc accataaata cctcccatct
      121 gaatttttga aatgtaaaaa atccagatgg agaggcggag aacgacaccg catatcagaa
      181 acagccctac ggcactttcc aacaaaaatc gacaaaagaa aaaccgcaaa ggctctgtga
      241 cctttacggt tataggaaat agtaattcta ttgtatggag attgtacttc ta
//
LOCUS       DASFSW010000376          290 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.denovo.380, whole genome shotgun sequence.
ACCESSION   DASFSW010000376 DASFSW010000000
VERSION     DASFSW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.denovo.380"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            36..104
                     /locus_tag="U1099_002344"
                     /pseudo
     CDS             36..104
                     /locus_tag="U1099_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          117 a           58 c           43 g           72 t
ORIGIN      
        1 tttattgagt atgaaaaaca acaaaactca caatttccaa caccaaatca gaaacaacta
       61 acaatctcat caaagtcatt aaaagaaatg cctttggatt caggaaaatg aaaactagaa
      121 aaacggattt tgatgctttg aacagtgaaa agctgaggag agctcttttc agctcatctc
      181 ctaaaaacaa gaaagagaga acgaatttcg tcctctctag gtgttagctt ttcatctacc
      241 cactacagtt gacaaagagc caaaaaaaaa gaacattgct actctcttac
//
LOCUS       DASFSW010000377         5223 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.guided.132, whole genome shotgun sequence.
ACCESSION   DASFSW010000377 DASFSW010000000
VERSION     DASFSW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.guided.132"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            147..1874
                     /locus_tag="U1099_002345"
     CDS             147..1874
                     /locus_tag="U1099_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868601.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDKAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKATQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGQGNFFI
                     NHQSVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEEGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            1874..3613
                     /locus_tag="U1099_002346"
     CDS             1874..3613
                     /locus_tag="U1099_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM4868602.1"
                     /translation="MTILGFLLKQIRRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     YGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
     gene            3667..4362
                     /locus_tag="U1099_002347"
     CDS             3667..4362
                     /locus_tag="U1099_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM4868603.1"
                     /translation="MKALIFDVDDTLYDQIQPFERALERHIEVAREQIEPLYLSFRRY
                     ADEVFEATAIGKMSLKDSHIYRMKHALADFGYQVSDATALAIQIDYDYFQGQIELSPV
                     FPEIFSWCQAQGIAMGIITNGPYRHQLRKIRTMGLVNWFELEHVLISGQVGITKPNPA
                     IFQLMEERLGMSGEDICYLGDSFENDVVGAKAANWKAIWFNHRKRVEPTAPYQADKVV
                     TDWDELVEVIQSF"
     gene            4516..5085
                     /locus_tag="U1099_002348"
     CDS             4516..5085
                     /locus_tag="U1099_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEM4868604.1"
                     /translation="MELPMTQAFFPIHTLETVSPELRENLATVKKNNGGYIPNLIGLL
                     ANSPTALETYQTVSGINRRSSLNPTEREVVQITAAVANGCGFCVAGHTAISIKQVKMP
                     DVILQALRQGTPIETDAKLDALARFTLAVIHEKGKVEQPLLEEFFQEGYTAENALDVV
                     LGVSLATLCNYANNLINTPINPELQAYAL"
BASE COUNT         1482 a          940 c         1216 g         1585 t
ORIGIN      
        1 ttgtcaactt gccttgatga actccagttc tatcttcgac atcgttttct aggctacttt
       61 tgattttcat tgagtataac gtctatcaat tgttgcttat tgaataaagc ggcaaatgat
      121 aggttataga tttgtaaagg agatatatgt ttcgattaat ttttgactat atcaagcgtc
      181 ataagtggct ctatctattg gttgctgtga ccttgattat ttatgacgcc acacttttgc
      241 taccaacgca aatcattcag cggatggtag atatactaac caagaatgaa ttaacacagg
      301 ccatattggt tcaggagatg accttattgt tattggtaac agtactgaac tatgcaacag
      361 ccttcatttg gcatctgaaa ttatttcagg cttctgtcaa tttcaagttt gatatgcagc
      421 aacgtgcctt taaaaaatta gtgaccatgc ggactccatt ttatgaaaaa ttccgctcag
      481 gagatgtcat gactcgtttt tcaacggatg tggatggcct gatggagatg gtgggttacg
      541 gtcttatgat tgtcgtctat gcaggcggta tgttggcttt tatcattccc acgatgttct
      601 ttattgattg gaaaatttcg ctagtagctt tactaccgat gctgttcatg acactatgta
      661 tattttttat cgggagaaaa caagataagg cgattgatgc caatagagaa gctgtagccc
      721 agctcaataa tgaagttttg gaggtcatcg aaggcattcg agtgactcgg gcttatagta
      781 aaaaagcaac tcaaaaggct caatttcaag ctcgtaccaa gcaattggct caaggtgggg
      841 atcgcatcac ttccttgcaa tcattgtata atccattagc cacagtctgt ttgggattgt
      901 ctactatttt cgtgcttctc atgggggcac aagccgtgaa agcagggcaa ctgaccttgg
      961 gacaagtaat tgcccttcaa ttgtatgtcg gttcgctttt ggagccgttt tggacactag
     1021 cagatttcat ccttgtttat cagactggta agacatcttt cgaaaaactg caagagctga
     1081 ttgagacggg tgatgatttg gaggctgatg gttctaagga aattgcagag ctgtctagta
     1141 tttccttcaa aaattatagc tttagttatc cacaggcgga aagagccagc ctacaggaca
     1201 tcaattggac actgaaagca gggcaaactg taggtatcgt tgggaaaact ggttcgggaa
     1261 aaacgacctt ggttcgccaa ttcttacgcc agtatccgat tggtcaggga aattttttca
     1321 tcaatcatca atctgtttta gactttaaac gttctagtat agaggaaaaa attggctatg
     1381 ttccccaaga acacattttg ttttcaaggt ctgttggaga aaatattgcc cttgggaaag
     1441 tagctagtag ctctgaggag attgaacaag ccattgccac agcagctttt agtcaagatt
     1501 taaaacggat gactgatggt cttgatacca tgattggtga gcgtggtgtg tctatttctg
     1561 gcggtcagaa acaacggatt tccattgccc gtgctttcct gcgagagcca gatttgctga
     1621 ttctagatga ttctttgtcg gcagtggatg ctaggacaga acgccagatt atccaaaata
     1681 ttcaaaaaga acgtgcaggc aaaaccaatg tcatcgtgac ccatcgtcta tctgctgtca
     1741 accatgcaga ctgggtacta gtcttggatg aggggcgtat cgttgaggaa gggcgtccag
     1801 ctgatttgct tgcccaaaga ggttggtact atgaacaata ccaacgacaa caaagtcagg
     1861 aaggaggaga gtaatgacta ttttaggttt tttattaaaa caaatccgtc gtgttaagtg
     1921 gctatttata gtagctgtcg gcttctatct attggcttcg accatggttc gattggctcc
     1981 tctactgatt cagcaggcga ttgatgggcc gattacggat ttgagcaagg gtttgccttt
     2041 tgatgaagct gttttcttaa accagtcagc tcaatacatg ggaatgatta ttctgggagc
     2101 tattggcttc tacttatcta tgcgtctact catgcattgc gccaatcgta ttgctgaaaa
     2161 tctacgtaat caagcttatg atgttatgca acggttgcct atttcttatt ttgatgataa
     2221 gcccgctgga aaaattgcaa ctagaattgt caatgataca gaaactctgc ggacacaatt
     2281 ttatgggacc ttagttaatg cctttaacaa tattgttcgt ttgctcttta catacggtgt
     2341 cctattttac atgaatagga gtcttggttg gctgatgtta ctcttgatac cactctatat
     2401 cggtattcaa tttgcttaca agaaaatgac agacaagccg atgaaggatt tttatgatgc
     2461 aaggagtgac gtcaatacgc aggtgaatga gaccatgaat ggtgcaagtt tgattcaact
     2521 ttttggacaa gaagagcgca tcatgaaaga atttgaagtg actgctgaca agatgcgtcg
     2581 ggcagataat aagattatct gggcccagtc tcttgccacg tggaatctga gtggttttct
     2641 tcaaaatttg gtcattgctg cgattttaac ggtagtcggc tatcagtttc ttgcgggaca
     2701 gtatggagtt acagctggga aactctttgt ttatgtcaac tatatcgaag gagtcttcat
     2761 tgctttggga gcgctggttc aacagttccc aaatatgctt cgctcgtttg aaacaggtaa
     2821 gcgtctaatg gcccttctag aagaagaggt ggaggacgac tgtgatcgta ttttggaagt
     2881 tgaacaaggg caagtcgtgt ttgaacacgt gaactttagc tatgaagaaa acagaccagt
     2941 tttgaaagat attaccattc gggcagaaaa aggagaaaca gttgctctgg ttgggcatac
     3001 tgggtctggt aagtcttcta ttatgaacct cctctatcgt ttctatgatc cacaggaggg
     3061 acgtgtactg attgatggga aaaacatccg tgattattct cgtgagagcc tgcgcagtca
     3121 catggggatt gttttgcaag atccctatct ctttacaggg acgattgcta gcaatgtttc
     3181 catgaatgaa gaggaagcag atagaacaag aatcatgcag gcgttagaaa aagttggtgc
     3241 agggcctatg ctatctcgct tagagaaagg gattgatgaa ccggttgttg agaagggggc
     3301 ggccttttca agtggggaac gtcagttgat tgcctttgcg cggacactct actctgatcc
     3361 taaaattctg atattggatg aagcaacttc tcatattgat acggagacgg aagaaattat
     3421 ccaacatgct atggaagtgg tgaaagaagg tcggacaacc tttatcattg cccaccgact
     3481 ttctaccatt caaaatgcag accagattct tgtcttagat caaggccgaa ttattgaaca
     3541 tgggaagcat gaagaattgg ttgcacgtgg tggagtatat gctcaaatgc atgagattca
     3601 agcaaaggtg taataagcag ttaaaaattc caagctttgg ctggaatttt ttgctataat
     3661 tgactcatga aagccttaat ttttgatgta gatgatacgc tttatgatca gattcaaccg
     3721 tttgaacgtg ctttggaaag acacattgaa gtagcaaggg agcaaattga accactctac
     3781 ctttcctttc gcagatatgc ggatgaagta tttgaagcga cagcaattgg aaagatgagt
     3841 ctgaaagaca gtcatattta tcggatgaaa catgctttgg cagattttgg gtatcaggtg
     3901 tctgatgcaa cagccttagc tattcaaata gattatgatt attttcaagg gcagatagag
     3961 ctcagtccag tgtttccaga aatattttca tggtgtcagg cgcaaggaat tgcaatgggg
     4021 attattacga atggacctta tagacatcag ttgagaaaga tacgcacgat ggggttagtt
     4081 aattggtttg agctagaaca tgtcctaatt tctggacaag ttggtatcac aaagccaaat
     4141 cctgctattt ttcaactaat ggaagaacgt ttggggatgt caggcgaaga tatttgttat
     4201 ctaggggatt cgtttgaaaa tgatgtggta ggagcgaaag ctgcgaattg gaaggcaatt
     4261 tggtttaatc atagaaagag agtagagcca acagctccgt atcaggcaga taaagtggtg
     4321 acagattggg atgaactggt cgaagttatc cagtctttct agtggtttct acaagggatt
     4381 atcaattttt tataggtgtg ataggtaaaa tatattagca ggatgcctgt ctttatgata
     4441 gtataaaatg caagcataga agtataggtg tgaatccaag cagattttta ttcctactct
     4501 atgaaatttt ttgaaatgga gttacctatg acacaagctt tctttcctat ccatactcta
     4561 gagactgtta gtccagaatt acgagaaaac cttgcgactg tcaagaaaaa taatggcggt
     4621 tatattccta atctcatcgg tcttttagcc aattctccta cagccctaga aacctaccaa
     4681 actgttagtg gtatcaatcg ccgtagtagt ctgaatccga cggagcgtga agtggtacaa
     4741 attacagctg ccgttgccaa tggctgtgga ttttgcgtgg caggtcatac ggctatttct
     4801 atcaagcagg ttaagatgcc tgatgttatt ctacaggctc tacgccaggg aactcccatc
     4861 gaaacggatg caaaattgga tgctctcgct cgatttactc tggctgttat ccatgaaaaa
     4921 ggcaaggttg aacagccctt gctagaggaa tttttccaag agggctacac ggctgaaaat
     4981 gccttggatg tggtgttagg agtcagtctt gcaactcttt gtaattatgc caacaatctc
     5041 atcaacactc ctatcaatcc agaattacag gcctatgctc tgtaggagtt ccagcagaat
     5101 atgtatcgca aactagttag gacgtttaca ttatcctaac tagttttttt gaattaacca
     5161 agtgtgattt tcctaatata catttgatac tcttcgaaaa tcaaaattat ccgttgtcaa
     5221 ctt
//
LOCUS       DASFSW010000378         2095 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.guided.226, whole genome shotgun sequence.
ACCESSION   DASFSW010000378 DASFSW010000000
VERSION     DASFSW010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.guided.226"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            6..1925
                     /gene="tet(O)"
                     /locus_tag="U1099_002349"
     CDS             6..1925
                     /gene="tet(O)"
                     /locus_tag="U1099_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HEM4868605.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPIKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYKERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            1977..>2095
                     /locus_tag="U1099_002350"
     CDS             1977..>2095
                     /locus_tag="U1099_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM4868606.1"
                     /translation="MMKCEWILCPVCGSKTRNKIRKDTVLENYPLYCPKCRQE"
BASE COUNT          690 a          359 c          484 g          562 t
ORIGIN      
        1 atcacatgaa aataattaac ttaggcattc tggctcacgt tgacgcagga aagacaacat
       61 taacggagag tttattgtat accagtggtg caattgcaga accagggagc gtagataaag
      121 gcacaacaag gacagataca atgaatttgg agcgtcaaag gggaatcact atccagacag
      181 cagtgacatc ttttcagtgg gaggatgtaa aagtcaacat tatagatacg ccaggccata
      241 tggatttttt ggcggaagta taccgttctt tatccgtatt agacggagca gtattattag
      301 tttctgcaaa ggatggcata caggcacaga cccgtatact gtttcatgca ctacagacaa
      361 tgaagattcc gacaattttt ttcatcaata aaattgacca agaggggatt gatttgccaa
      421 tggtatatca agaaatgaaa gcaaagcttt cttcggaaat tatagtgaag caaaaggttg
      481 ggcagcatcc ccatataaat gtaacggaca atgacgatat ggaacagtgg gatgcggtaa
      541 ttatgggaaa cgatgaacta ttagagaaat atatgtcagg gaaaccgatt aaaatgtcag
      601 aactggaaca ggaagaaaac aggagattcc aaaacggaac gttatttccc gtttatcacg
      661 gaagtgctaa aaacaatctg gggattcggc agcttataga agtgattgcc agtaagtttt
      721 attcatcaac gcctgaaggt caatctgaac tatgcgggca ggtttttaag attgaatatt
      781 cagagaaaag gcggcgtttt gtttatgtgc gtatatatag cggaacattg catttgaggg
      841 atgttattaa aatatctgaa aaagagaaaa taaaaatcac agagatgtgt gttccgacaa
      901 acggtgaatt atattcatcc gatacagcct gctctggtga tattgtaatt ttaccaaatg
      961 atgttttgca gctaaacagt attttgggga acgaaatgct gttgccgcag agaaaattta
     1021 ttgaaaatcc tctccctatg ctccaaacaa cgattgcagt aaagaaatct gaacagcggg
     1081 aaatattgct tggggcactt acagaaattt cagatggcga ccctctttta aaatattatg
     1141 tggatactac aacgcatgag attatacttt cttttttggg gaatgtgcag atggaagtca
     1201 tttgtgccat ccttgaggaa aaataccatg tggaggcaga aataaaagag cctactgtta
     1261 tatataagga aagaccgctt agaaaagcag aatataccat ccacatagaa gtcccgccaa
     1321 atcctttctg ggcttctgtc gggttgtcca tagagccgct ccctattgga agcggagtgc
     1381 agtatgaaag cagagtttca cttggatatt taaaccaatc gttccaaaat gcggttatgg
     1441 agggggttct ttatggctgc gagcaggggc tgtatggatg gaaagtgaca gactgtaaaa
     1501 tctgttttga atatggattg tattatagtc ctgtaagtac ccccgcagac tttcggctgc
     1561 tttcccctat cgtattggag caggctttaa aaaaagcagg gacagaacta ttagagccat
     1621 atctccactt tgaaatttat gcaccgcagg aatatctctc acgggcgtat catgatgccc
     1681 caaggtattg tgcagatatt gtaagtactc aggtaaagaa tgacgaggtc attctgaaag
     1741 gagaaatccc tgccagatgt attcaagaat acaggaacga tttaacttat ttcacaaatg
     1801 ggcagggagt ctgcttgaca gagttaaaag gataccagcc agctattggt aaatttattt
     1861 gccaaccccg ccgcccgaat agccgtatag ataaggttcg gcatatgttc cacaagttag
     1921 cttaacagct tgcaaaagtc atataaaatg agatttgaaa ggattagaga ctaattatga
     1981 tgaaatgcga atggatattg tgtcctgttt gtgggagcaa aacccgtaat aaaattagga
     2041 aggacactgt tttggagaat tatccccttt attgtccaaa atgcagacaa gaaag
//
LOCUS       DASFSW010000379         2095 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.guided.227, whole genome shotgun sequence.
ACCESSION   DASFSW010000379 DASFSW010000000
VERSION     DASFSW010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.guided.227"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            6..1925
                     /gene="tet(O)"
                     /locus_tag="U1099_002351"
     CDS             6..1925
                     /gene="tet(O)"
                     /locus_tag="U1099_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HEM4868607.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYKERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            1977..>2095
                     /locus_tag="U1099_002352"
     CDS             1977..>2095
                     /locus_tag="U1099_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM4868608.1"
                     /translation="MMKCEWILCPVCGSKTRNKIRKDTVLENYPLYCPKCRQE"
BASE COUNT          689 a          359 c          484 g          563 t
ORIGIN      
        1 atcacatgaa aataattaac ttaggcattc tggctcacgt tgacgcagga aagacaacat
       61 taacggagag tttattgtat accagtggtg caattgcaga accagggagc gtagataaag
      121 gcacaacaag gacagataca atgaatttgg agcgtcaaag gggaatcact atccagacag
      181 cagtgacatc ttttcagtgg gaggatgtaa aagtcaacat tatagatacg ccaggccata
      241 tggatttttt ggcggaagta taccgttctt tatccgtatt agacggagca gtattattag
      301 tttctgcaaa ggatggcata caggcacaga cccgtatact gtttcatgca ctacagacaa
      361 tgaagattcc gacaattttt ttcatcaata aaattgacca agaggggatt gatttgccaa
      421 tggtatatca agaaatgaaa gcaaagcttt cttcggaaat tatagtgaag caaaaggttg
      481 ggcagcatcc ccatataaat gtaacggaca atgacgatat ggaacagtgg gatgcggtaa
      541 ttatgggaaa cgatgaacta ttagagaaat atatgtcagg gaaaccgttt aaaatgtcag
      601 aactggaaca ggaagaaaac aggagattcc aaaacggaac gttatttccc gtttatcacg
      661 gaagtgctaa aaacaatctg gggattcggc agcttataga agtgattgcc agtaagtttt
      721 attcatcaac gcctgaaggt caatctgaac tatgcgggca ggtttttaag attgaatatt
      781 cagagaaaag gcggcgtttt gtttatgtgc gtatatatag cggaacattg catttgaggg
      841 atgttattaa aatatctgaa aaagagaaaa taaaaatcac agagatgtgt gttccgacaa
      901 acggtgaatt atattcatcc gatacagcct gctctggtga tattgtaatt ttaccaaatg
      961 atgttttgca gctaaacagt attttgggga acgaaatgct gttgccgcag agaaaattta
     1021 ttgaaaatcc tctccctatg ctccaaacaa cgattgcagt aaagaaatct gaacagcggg
     1081 aaatattgct tggggcactt acagaaattt cagatggcga ccctctttta aaatattatg
     1141 tggatactac aacgcatgag attatacttt cttttttggg gaatgtgcag atggaagtca
     1201 tttgtgccat ccttgaggaa aaataccatg tggaggcaga aataaaagag cctactgtta
     1261 tatataagga aagaccgctt agaaaagcag aatataccat ccacatagaa gtcccgccaa
     1321 atcctttctg ggcttctgtc gggttgtcca tagagccgct ccctattgga agcggagtgc
     1381 agtatgaaag cagagtttca cttggatatt taaaccaatc gttccaaaat gcggttatgg
     1441 agggggttct ttatggctgc gagcaggggc tgtatggatg gaaagtgaca gactgtaaaa
     1501 tctgttttga atatggattg tattatagtc ctgtaagtac ccccgcagac tttcggctgc
     1561 tttcccctat cgtattggag caggctttaa aaaaagcagg gacagaacta ttagagccat
     1621 atctccactt tgaaatttat gcaccgcagg aatatctctc acgggcgtat catgatgccc
     1681 caaggtattg tgcagatatt gtaagtactc aggtaaagaa tgacgaggtc attctgaaag
     1741 gagaaatccc tgccagatgt attcaagaat acaggaacga tttaacttat ttcacaaatg
     1801 ggcagggagt ctgcttgaca gagttaaaag gataccagcc agctattggt aaatttattt
     1861 gccaaccccg ccgcccgaat agccgtatag ataaggttcg gcatatgttc cacaagttag
     1921 cttaacagct tgcaaaagtc atataaaatg agatttgaaa ggattagaga ctaattatga
     1981 tgaaatgcga atggatattg tgtcctgttt gtgggagcaa aacccgtaat aaaattagga
     2041 aggacactgt tttggagaat tatccccttt attgtccaaa atgcagacaa gaaag
//
LOCUS       DASFSW010000380         1293 bp    DNA     linear   BCT 11-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate 224_43
            SAMN09459705-rid19047253.guided.267, whole genome shotgun sequence.
ACCESSION   DASFSW010000380 DASFSW010000000
VERSION     DASFSW010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09459705
            Sequence Read Archive: SRR7369618
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001847095.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001847095.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/12/2023 02:28:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,354
            CDSs (total)                      :: 2,321
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 22 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09459705-rid19047253.guided.267"
                     /isolate="224_43"
                     /isolation_source="lung"
                     /host="Sus scrofa"
                     /db_xref="taxon:1307"
                     /country="Australia"
                     /collection_date="2017"
     gene            94..831
                     /gene="erm(B)"
                     /locus_tag="U1099_002353"
     CDS             94..831
                     /gene="erm(B)"
                     /locus_tag="U1099_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM4868609.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            977..1261
                     /locus_tag="U1099_002354"
     CDS             977..1261
                     /locus_tag="U1099_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEM4868610.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
BASE COUNT          485 a          215 c          214 g          379 t
ORIGIN      
        1 tcttatgact ttttaaacag ataactaaaa ttacaaacaa atcgtttaac ttctgtattt
       61 gtttatagat gtaatcactt caggagtgat taaatgaaca aaaatataaa atattctcaa
      121 aactttttaa cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa
      181 accgataccg tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa
      241 ataagtaaac aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa
      301 aaattaaaac tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc
      361 cctaacaaac agaggtataa aattgttggg aatattcctt accatttaag cacacaaatt
      421 attaaaaaag tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga
      481 ttctacaagc gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc
      541 tcgattcagc aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac
      601 agtgtcttaa taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta
      661 tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat
      721 cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag
      781 caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag
      841 tcgcttttgt aaatttggaa agttacacgt tactaaaggg aatgtagata aattattagg
      901 tatactactg acagcttcca aggagctaaa gaggtcccta gcgcctacgg ggaatttgta
      961 tcgataaggg gtacaaattc ccactaagcg ctcgggaccc cttgtaggaa aatgtcctaa
     1021 gtgtggcaac aatattgtat taaaaaaatc gttttatggt tgttcaaatt atcctgaatg
     1081 taccttcact ttagctgaac attttagaaa gaaaaaactc accaaaacaa atgtaaaaga
     1141 attactagag ggaaaagaaa ccctggtaaa aggaatcaaa acgaaagata gaaagtccta
     1201 caatgccgtt gtaaaaatcg gagaaaaggg atatattgat tttatctctt tttcaaaata
     1261 agcataaaag ccctttaaag agggctttta tat
//
